LOCUS       DAWFAD010000001        39765 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWFAD010000001 DAWFAD010000000
VERSION     DAWFAD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39765
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.001"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(91..864)
                     /locus_tag="WGO49_000001"
     CDS             complement(91..864)
                     /locus_tag="WGO49_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ0305174.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKAGLQSGVYQVLLGDADVKSAAVRGKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
     gene            complement(963..1586)
                     /gene="rsmG"
                     /locus_tag="WGO49_000002"
     CDS             complement(963..1586)
                     /gene="rsmG"
                     /locus_tag="WGO49_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ0305175.1"
                     /translation="MERKERLRAGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTGHLLKDG
                     GYWAAMKGVYPQGEIGRLPQDVCVEKVQRLDVPGLDAERHIVILSKR"
     gene            complement(1682..2167)
                     /locus_tag="WGO49_000003"
     CDS             complement(1682..2167)
                     /locus_tag="WGO49_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305176.1"
                     /translation="MYVNEKYPYAALFAGLVFLTLPFALSVHDALAFAFGRAGLLVSV
                     SDGEFGWRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSAALGGLRGEAVSEYAFAVWLVSMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            2272..2347
                     /locus_tag="WGO49_000004"
     tRNA            2272..2347
                     /locus_tag="WGO49_000004"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2305..2307,aa:Thr,seq:cgt)
     gene            2421..3548
                     /locus_tag="WGO49_000005"
     CDS             2421..3548
                     /locus_tag="WGO49_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ0305177.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            3729..4286
                     /gene="frr"
                     /locus_tag="WGO49_000006"
     CDS             3729..4286
                     /gene="frr"
                     /locus_tag="WGO49_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ0305178.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            4342..5088
                     /locus_tag="WGO49_000007"
     CDS             4342..5088
                     /locus_tag="WGO49_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ0305179.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDET
                     ALDAAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            5092..5889
                     /locus_tag="WGO49_000008"
     CDS             5092..5889
                     /locus_tag="WGO49_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ0305180.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLVATLVFGVVAYAGGWMLPNLVWYVVLAFWLAIMPLWLRFKWRLN
                     GGWQVYAVGWLLIMPFWFALVSLRPHPDNALPLLAVMGLVWIADVFAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSKLLPGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            5894..7129
                     /locus_tag="WGO49_000009"
     CDS             5894..7129
                     /locus_tag="WGO49_000009"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ0305181.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            7164..8504
                     /gene="rseP"
                     /locus_tag="WGO49_000010"
     CDS             7164..8504
                     /gene="rseP"
                     /locus_tag="WGO49_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ0305182.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLNYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            8561..10954
                     /gene="bamA"
                     /locus_tag="WGO49_000011"
     CDS             8561..10954
                     /gene="bamA"
                     /locus_tag="WGO49_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ0305183.1"
                     /translation="MKLKQIASALMMLGISPLAFADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEVGIAGGYGRTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            11020..11520
                     /locus_tag="WGO49_000012"
     CDS             11020..11520
                     /locus_tag="WGO49_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ0305184.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            11553..12599
                     /gene="lpxD"
                     /locus_tag="WGO49_000013"
     CDS             11553..12599
                     /gene="lpxD"
                     /locus_tag="WGO49_000013"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ0305185.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPSATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            12633..13082
                     /gene="fabZ"
                     /locus_tag="WGO49_000014"
     CDS             12633..13082
                     /gene="fabZ"
                     /locus_tag="WGO49_000014"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ0305186.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVS
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            13289..13969
                     /locus_tag="WGO49_000015"
     CDS             13289..13969
                     /locus_tag="WGO49_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ0305187.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDATGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINKFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            14012..14788
                     /gene="lpxA"
                     /locus_tag="WGO49_000016"
     CDS             14012..14788
                     /gene="lpxA"
                     /locus_tag="WGO49_000016"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ0305188.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRHAETQAELAVFRDFFAQSARGIIR"
     gene            15146..16537
                     /locus_tag="WGO49_000017"
     CDS             15146..16537
                     /locus_tag="WGO49_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0305189.1"
                     /translation="MQVFLDNPKAFFETVSGWVWGPVMLMLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFVPPKKHKSGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMALA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            16616..17872
                     /locus_tag="WGO49_000018"
     CDS             16616..17872
                     /locus_tag="WGO49_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ0305190.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAKGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWRMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETETGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQALFWSGLRPMTPDSTPLIGRTRFENLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            18053..18862
                     /gene="zupT"
                     /locus_tag="WGO49_000019"
     CDS             18053..18862
                     /gene="zupT"
                     /locus_tag="WGO49_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ0305191.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            18959..21796
                     /locus_tag="WGO49_000020"
     CDS             18959..21796
                     /locus_tag="WGO49_000020"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ0305192.1"
                     /translation="MLDKYSPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLEKLLVKRTTGLRKPETAPKVEEASRKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVDRL
                     NQTIEQVTQAYETYRFDLAAETLYSFMWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGDIVQTAFEQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESTDDLADYLKYLPMMTRLIEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            21938..22129
                     /locus_tag="WGO49_000021"
     CDS             21938..22129
                     /locus_tag="WGO49_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305193.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(22126..23046)
                     /gene="oxyR"
                     /locus_tag="WGO49_000022"
     CDS             complement(22126..23046)
                     /gene="oxyR"
                     /locus_tag="WGO49_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ0305194.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(23050..23313)
                     /gene="minE"
                     /locus_tag="WGO49_000023"
     CDS             complement(23050..23313)
                     /gene="minE"
                     /locus_tag="WGO49_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ0305195.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            complement(23317..24132)
                     /gene="minD"
                     /locus_tag="WGO49_000024"
     CDS             complement(23317..24132)
                     /gene="minD"
                     /locus_tag="WGO49_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ0305196.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            complement(24160..24873)
                     /gene="minC"
                     /locus_tag="WGO49_000025"
     CDS             complement(24160..24873)
                     /gene="minC"
                     /locus_tag="WGO49_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ0305197.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            complement(25020..25388)
                     /gene="rplQ"
                     /locus_tag="WGO49_000026"
     CDS             complement(25020..25388)
                     /gene="rplQ"
                     /locus_tag="WGO49_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ0305198.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            complement(25412..26398)
                     /gene="rpoA"
                     /locus_tag="WGO49_000027"
     CDS             complement(25412..26398)
                     /gene="rpoA"
                     /locus_tag="WGO49_000027"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ0305199.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            complement(26424..27044)
                     /gene="rpsD"
                     /locus_tag="WGO49_000028"
     CDS             complement(26424..27044)
                     /gene="rpsD"
                     /locus_tag="WGO49_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ0305200.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            complement(27064..27459)
                     /gene="rpsK"
                     /locus_tag="WGO49_000029"
     CDS             complement(27064..27459)
                     /gene="rpsK"
                     /locus_tag="WGO49_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ0305201.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            complement(27479..27841)
                     /gene="rpsM"
                     /locus_tag="WGO49_000030"
     CDS             complement(27479..27841)
                     /gene="rpsM"
                     /locus_tag="WGO49_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ0305202.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            complement(27907..28020)
                     /gene="rpmJ"
                     /locus_tag="WGO49_000031"
     CDS             complement(27907..28020)
                     /gene="rpmJ"
                     /locus_tag="WGO49_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ0305203.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            complement(28041..28259)
                     /gene="infA"
                     /locus_tag="WGO49_000032"
     CDS             complement(28041..28259)
                     /gene="infA"
                     /locus_tag="WGO49_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ0305204.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            complement(28264..29574)
                     /gene="secY"
                     /locus_tag="WGO49_000033"
     CDS             complement(28264..29574)
                     /gene="secY"
                     /locus_tag="WGO49_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ0305205.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            complement(29586..30020)
                     /gene="rplO"
                     /locus_tag="WGO49_000034"
     CDS             complement(29586..30020)
                     /gene="rplO"
                     /locus_tag="WGO49_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ0305206.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            complement(30022..30207)
                     /gene="rpmD"
                     /locus_tag="WGO49_000035"
     CDS             complement(30022..30207)
                     /gene="rpmD"
                     /locus_tag="WGO49_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ0305207.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            complement(30200..30718)
                     /gene="rpsE"
                     /locus_tag="WGO49_000036"
     CDS             complement(30200..30718)
                     /gene="rpsE"
                     /locus_tag="WGO49_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ0305208.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            complement(30737..31090)
                     /gene="rplR"
                     /locus_tag="WGO49_000037"
     CDS             complement(30737..31090)
                     /gene="rplR"
                     /locus_tag="WGO49_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ0305209.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            complement(31104..31637)
                     /gene="rplF"
                     /locus_tag="WGO49_000038"
     CDS             complement(31104..31637)
                     /gene="rplF"
                     /locus_tag="WGO49_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ0305210.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            complement(31651..32043)
                     /gene="rpsH"
                     /locus_tag="WGO49_000039"
     CDS             complement(31651..32043)
                     /gene="rpsH"
                     /locus_tag="WGO49_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ0305211.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            complement(32059..32364)
                     /gene="rpsN"
                     /locus_tag="WGO49_000040"
     CDS             complement(32059..32364)
                     /gene="rpsN"
                     /locus_tag="WGO49_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ0305212.1"
                     /translation="MAKKALINRNLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            complement(32367..32906)
                     /gene="rplE"
                     /locus_tag="WGO49_000041"
     CDS             complement(32367..32906)
                     /gene="rplE"
                     /locus_tag="WGO49_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ0305213.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            complement(32916..33239)
                     /gene="rplX"
                     /locus_tag="WGO49_000042"
     CDS             complement(32916..33239)
                     /gene="rplX"
                     /locus_tag="WGO49_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ0305214.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            complement(33251..33619)
                     /gene="rplN"
                     /locus_tag="WGO49_000043"
     CDS             complement(33251..33619)
                     /gene="rplN"
                     /locus_tag="WGO49_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ0305215.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(33841..34104)
                     /gene="rpsQ"
                     /locus_tag="WGO49_000044"
     CDS             complement(33841..34104)
                     /gene="rpsQ"
                     /locus_tag="WGO49_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ0305216.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            complement(34104..34295)
                     /gene="rpmC"
                     /locus_tag="WGO49_000045"
     CDS             complement(34104..34295)
                     /gene="rpmC"
                     /locus_tag="WGO49_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ0305217.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            complement(34295..34711)
                     /gene="rplP"
                     /locus_tag="WGO49_000046"
     CDS             complement(34295..34711)
                     /gene="rplP"
                     /locus_tag="WGO49_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ0305218.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            complement(34695..35387)
                     /gene="rpsC"
                     /locus_tag="WGO49_000047"
     CDS             complement(34695..35387)
                     /gene="rpsC"
                     /locus_tag="WGO49_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ0305219.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            complement(35397..35726)
                     /gene="rplV"
                     /locus_tag="WGO49_000048"
     CDS             complement(35397..35726)
                     /gene="rplV"
                     /locus_tag="WGO49_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ0305220.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            complement(35735..36013)
                     /gene="rpsS"
                     /locus_tag="WGO49_000049"
     CDS             complement(35735..36013)
                     /gene="rpsS"
                     /locus_tag="WGO49_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ0305221.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            complement(36020..36853)
                     /gene="rplB"
                     /locus_tag="WGO49_000050"
     CDS             complement(36020..36853)
                     /gene="rplB"
                     /locus_tag="WGO49_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ0305222.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     NNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            complement(36859..37173)
                     /gene="rplW"
                     /locus_tag="WGO49_000051"
     CDS             complement(36859..37173)
                     /gene="rplW"
                     /locus_tag="WGO49_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ0305223.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANAAKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            complement(37170..37790)
                     /gene="rplD"
                     /locus_tag="WGO49_000052"
     CDS             complement(37170..37790)
                     /gene="rplD"
                     /locus_tag="WGO49_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ0305224.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            complement(37790..38434)
                     /gene="rplC"
                     /locus_tag="WGO49_000053"
     CDS             complement(37790..38434)
                     /gene="rplC"
                     /locus_tag="WGO49_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ0305225.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            complement(38888..39317)
                     /locus_tag="WGO49_000054"
                     /pseudo
     CDS             complement(38888..39317)
                     /locus_tag="WGO49_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(39438..39749)
                     /gene="rpsJ"
                     /locus_tag="WGO49_000055"
     CDS             complement(39438..39749)
                     /gene="rpsJ"
                     /locus_tag="WGO49_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ0305226.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLSAGVDVEIKV
                     Q"
BASE COUNT        10490 a        10887 c         8981 g         9407 t
ORIGIN      
        1 atgacgatat aagtattttt attttaatcc gccatattaa cgcacccggc caaacagcat
       61 aaaggcacgg gcagcccgat ttggattgac ctatttcccc gacaccctcg ccatcagctc
      121 gtccgccaag gcaagatacg ccttggtacc ctttgcctgc gcgtcgtaag ccatcaccgg
      181 cataccgtgg ctcggtgctt ccgcaaggcg gatattgcgc gggatgacgg tttcaaacag
      241 caaatccccg aaatggctgc gcaactgttc gctgacttcg gcaaccagcc tgctgcggct
      301 gtcgtacatc gtgcgcacga tgcccgtgat gtccaaatcg ggattgaccg cctgacggat
      361 tttacgcacg gtcgcaatca aatcggaaat cccctcaagc gcgtaatatt cgcacaacat
      421 cggcacaatc acgccgcccg ccgccaccaa gccgttaagc gtcaacagcg tcagcgaagg
      481 cggacagtcg atcaggataa agtcgtaatc ttccgccact gccttgagtg cgtttttcaa
      541 acgcacttcc cgggcgattt cctgcaccag ttcgatttcc gcgccggcaa gtgcgcggtt
      601 cgcacccaac acagcgtatc cgccctcttt gccgcgtacc gccgccgatt tcacgtccgc
      661 atcgcccaat aagacctgat aaacgccgga ctgcaaaccc gccttgtcga taccgctgcc
      721 cgtcgtcgca ttgccctgcg gatccaaatc gaccaccagc acgcgtttgc cgcgcgatgc
      781 cagcgaagcc gccaaattta ccgtcgtcgt cgttttgccc acaccgccct tctgattggc
      841 gatggcaagg atgttcgcac tcatgtcccg cccgtgtccc gtctgatgtt gaaaaaagaa
      901 aaacagtgcg gaattttacc tttatccgca caaaaaaggt attcatgccg tctgaagtgc
      961 gctcaacgct tgctcaggat gacgatatgg cgttccgcat ccaagcctgg cacgtcgagc
     1021 ctttggactt tttcaacgca cacatcctgc ggcaggcggc cgatttcccc ctgcggatac
     1081 acgcccttca tcgccgccca gtatccgccg tctttcaaca gatgccccgt ccacgacaca
     1141 aagtccgcca gttctgcaaa cgcacggctg gtaaccacat cggcacgcac gtccgaaacc
     1201 gcctccacgc gtccggatac cacgcgcaca ttgtccaacc ccaactcgat aaccgcctgc
     1261 tgtaaaaaag ccgttttctt cgtatttgca tccaaaaggg ttatctgcac atccggacgg
     1321 cacaccgccg ccggaatgcc gggctgaccg ccgcccgaac cgacatccag catcgtttgc
     1381 acaccctcga tatggggcag cagcgtcagg ctgtccaaaa gatgatggac aatcattttt
     1441 tcctcgtcgc gcagggcggt cagattgtag gttttgttcc actttttcaa caaatccaca
     1501 taggccaaaa gcctgtcctg cgccgtttcc gaaatatcca gccccatcgc ggcaatgcct
     1561 gcacgcaggc gttctttgcg ttccatatct gcttcctgtc catattcagg cgtaatgtta
     1621 gcggaaaaac atgccatctg aaaccgcgtg tcggggttca gacggcattt cccgttccaa
     1681 cctatttttt cctgtgaaac accaccggct gcaccggcgc gcgcgtcagg cgtttgggca
     1741 gcgtcagcat agacaccagc cacacggcaa acgcatattc cgacaccgcc tccccgcgca
     1801 gcccgcccaa ggcggcactg cccaacaaca gctcgaaaat ccacgcgacc gcagcagccg
     1861 cgcaagccgc cacgcccgac agaaacagcg cgtaacgcga atgccgccgc atcatctttt
     1921 tgtacgccag tttcgtcagg cccgccgaca caaccacatt caaaaacgca aacacaccga
     1981 acacaaacca aacagtgccg tcccaaccgc cacgccagcc gaattcgccg tccgacaccg
     2041 acaccagcag ccccgcccgt ccgaacgcaa atgccaaggc atcatgcacc gacagtgcga
     2101 acggcagcgt caaaaacacc agccccgcaa acaaagccgc atagggatat ttctcgttca
     2161 catacatcac cgactctcct cacgaagcaa accgcattat accgtgcaaa actttgccag
     2221 cagcccgaaa agttgcagaa acaaccgttc atatatatca taacgaaaaa agccggtgta
     2281 gctcagtcgg tagagcagcg cattcgtaac gcgaaggtcg ggggttcgat tcccttctcc
     2341 ggcaccaata ccaagcacag accctccctt cctcgggaag cctgtgcttt ttcacatttc
     2401 cgcttcagac ggcaaccgat atgaacacct cgcaacgcaa ccgcctcgtc agccgctggc
     2461 tcaactccta cgaacgctac cgccaccgcc gcctcataca cgccgtgcgg ctcggcgggg
     2521 ccgtcctgtt cgccaccgca ctcgcccggc tgctccacct ccaacacggc gaatggatag
     2581 gcatgaccgt cttcgtcgtc ctcggcatgc tccagtttca aggggcgatt tactccaagg
     2641 cggtggaacg tatgctcggc acggtcatcg ggctgggcgc gggtttgggc gttttatggc
     2701 tgaaccagca ttatttccac ggcaacctcc tcttctacct caccgtcggc acggcaagcg
     2761 cactggccgg ctgggcggcg gtcggcaaaa acggctacgt ccctatgctg gcggggctga
     2821 cgatgtgcat gctcatcggc gacaacggca gcgaatggtt cgacagcggc ctgatgcgcg
     2881 ccatgaacgt cctcatcggc gcggccatcg ccattgccgc cgccaaactg ctgccgctga
     2941 aatccacact gatgtggcgt ttcatgcttg ccgacaacct gaccgactgc agcaaaatga
     3001 ttgccgaaat cagcaacggc aggcgtatga cgcgcgaacg tttggagcag aatatggtta
     3061 aaatgcgcca aatcaacgca cgcatggtca aaagccgcag ccacctcgcc gccacatcgg
     3121 gcgaaagccg catcagcccc gccatgatgg aagccatgca gcacgcccac cgtaaaattg
     3181 tcaacaccac cgagctgctc ctgaccactg ccgccaagct gcaatctccc aaactcaacg
     3241 gcagcgaaat ccggctgctc gaccgccact tcacactgct ccaaaccgac ctgcaacaaa
     3301 ccgtcgccct tatcaacggc agacacgccc gccgcatccg catcgacacc gccatcaacc
     3361 ccgaactgga agccctcgcc gaacacctcc actaccaatg gcagggcttc ctctggctca
     3421 gcaccaatat gcgtcaggaa atttccgccc tcgtcatcct gctgcaacgc acccgccgca
     3481 aatggctgga tgcccacgaa cgccaacacc tgcgccaaag cctgcttgaa acacgggaac
     3541 acggctgacg gtcggacacg atgccgtctg aaacgcctgc cgcttcagac ggcatcccca
     3601 atccgcctgc cttgtcgtca tgcccgaata acggtactat tcgcagttaa cagttttcat
     3661 gccgtctgaa aggtttgaag cgcgtttcag acggctgccc gcccacctta tcccaccgaa
     3721 agaacaatat gatcaacgat attcaaaaaa cagccgaagg caaaatgcag cgttcggtcg
     3781 aagtactgaa agaaaatctg gcgaaagtgc gtaccggccg cgcgcatacc ggcctgctcg
     3841 accaagtgga agtcgaatac tggggcagca tggtccccgt cagccaagtt gccaacgtaa
     3901 cgcttctgga cgcgcgcacc atcggcgtga aaccgtttga gggcaatatg gcggccaaag
     3961 tcgagaaagc catccgcgat tcaaacttgg ggctgaaccc ggcagctgtc ggcgacctga
     4021 tccgcgtgcc gatgcccatg ctgaccgagg aacgccgcaa agacctgatt aaagtcgtac
     4081 gcggcgaagc ggaagaaggc cgcgtctcta tccgcaacgt gcgccgcgat gccaacgacc
     4141 acatcaaaaa actcctcaaa gacaaagaaa tttccgaaga cgaggcacgt cgcggcgaag
     4201 aagcggttca aaaactgacc gacaaataca ttaccgaagc cgacaaactc ctgactgcca
     4261 aagaagaaga tttgatggca atttaacctg cacggttcgg cgttcagacg gcatttgaat
     4321 gccgaaccgc gaaaggcaga catgaaaagc agcacgcagg ccgttttgga acacaccgcc
     4381 attcccaagc atatcgccgt gattatggac ggcaacggcc gttgggcgaa aaaacgtttc
     4441 ctcccgcgca taatgggaca caaacgcggt ttggacgcat tggaaaatat ggtgaagcat
     4501 tgcgccaaac tgggtgtgca atatctgacc gtgtttgcct tttcaaccga aaactggcgc
     4561 cgccccgaag acgaagtttc gttcctgatg gggctgtttt tacaggcttt gcaaaaacag
     4621 gtacgccgtc tgcacgaaaa caatatgcgc ctgaaaatac tgggcagccg cgaacgcttc
     4681 aaccggcaga ttctgcaagg catcgaagaa gcggaggcgt tgacggcaaa caataccggc
     4741 ctgaccctga gcattgccgc cgattacggc ggccgctggg atattttgca ggcggcaaac
     4801 aaactgattg ccgaaggcgt atccgagatt acagaagaca cgctggcgaa acacttgatg
     4861 ctgggcgatg caccggaacc ggatttgttc atccgcaccg gcggagaaac gcgcatcagc
     4921 aatttcctgc tctggcagat ggcgtatgca gaactgtatt tcaccgacat cctgtggcct
     4981 gatttcgacg aaaccgcctt agatgccgcc gtcgcttcgt tccaaaaacg cgaacggcgg
     5041 ttcggacgca cctccgagca actgcctatc gaacagcaaa ggaactaaat tatgctgaaa
     5101 caacgggtaa taaccgctat gtggctgctg ccgctgatgc tgggcatgct gttttacgcg
     5161 ccgcaatggt tgtgggctgc attttgcgga ctgattgccc tgattgcctt gtgggaatat
     5221 gcccgtatgg gcggcttgtg caaaatcaaa accaaccatt acctcgtcgc aaccttggtt
     5281 ttcggcgtgg ttgcctatgc gggcggctgg atgctgccta atttggtttg gtatgttgtt
     5341 ttggcatttt ggctcgccat catgccttta tggttgagat ttaaatggag gctcaacggc
     5401 ggttggcagg tttatgccgt cggctggctt ctgatcatgc cgttttggtt cgcgctcgta
     5461 tccctgcgcc cgcatcccga taatgccctg ccgctgctcg ccgtcatggg tttggtgtgg
     5521 attgccgatg ttttcgcgta tttcagcggc aaggcgttcg gcaaacacaa aatcgcaccg
     5581 gcaatcagcc ccggcaagag ctgggaaggc gcaatcggcg gcgcggtttg cgtggccgtg
     5641 tacatgaccg ccgtacgaag tgccggctgg ctggcattcg atacaggctg gttcgatacc
     5701 gtgttaatcg gtttggtgtt gaccgttgtc agcgtatgcg gcgacctttt ggaaagctgg
     5761 ctcaagcgcg cggcaggcat caaagacagc agcaagctac tgccaggaca cggcggcgtg
     5821 ttcgaccgta ccgacagcct gattgccgtt atcagcgtct atgcggcgat gatgtcggtt
     5881 ttaaattgat tctatgccgt ctgaaaacgc ttcagacggc atccggtata aagttatcct
     5941 cattatgaca ccacaagtcc tgaccatatt aggcagtacc ggcagcatag gcgaaagcac
     6001 gctggacgtt gtctcccgcc accccgaaaa attccgcgta ttcgcgctgg cagggcataa
     6061 gcaggtcgag aaattggcgg ctcaatgtca aacgttccgc cccgaatatg ccgtcgttgc
     6121 cgatgcggaa cacgccgctc ggcttgaagc cctgttgaaa cgcgacggca cggcgacgca
     6181 ggttttacac ggcgcgcagg cattggttga cgttgcgtct gccgacgaag tcagcggtgt
     6241 catgtgcgcc atcgtcgggg cagtggggct gccttccgcg ctcgcagcgg cgcaaaaagg
     6301 caaaaccatt tatctggcga acaaagagac gctggtggtt tccggcgcgt tgtttatgga
     6361 aaccgcccgt gcaaacggcg cggcagtgct gcccgtcgac agcgaacaca acgccgtttt
     6421 ccaagttttg ccgcgcgatt acgccggccg tctgaacgaa cacggcatcg cttcgattat
     6481 cctgaccgct tccggcggcc cgtttctgac cgccgattta aacacgttcg acagcattac
     6541 gcccgcccaa gcggtcaaac accccaattg gagcatgggg cgcaaaatct ccgtcgattc
     6601 cgccaccatg atgaacaaag gtttggagct gattgaagcg cattggctgt tcaactgtcc
     6661 gcctgacaag ctcgaagtcg tcatccatcc gcaatccgtg atacacagca tggtgcgcta
     6721 ccgcgacggc tccgtgttgg cacaactggg caatcccgat atgcgaacgc ccatcgccta
     6781 ttgtttgggc ttgcccgagc gcatcgattc gggtgtcggc gaccttgatt tcgacgcatt
     6841 gtccgcgctg accttccaaa agcccgactt tgaccgcttc ccctgcctga agctcgccta
     6901 tgaagccatg aacgcaggcg gagccgcgcc ctgcgtattg aacgccgcca acgaagccgc
     6961 cgtcgccgcc tttttggacg gacagattaa gtttaccgac attgccaaaa ccgtcgccca
     7021 ttgtctttca caagactttt cagacggcat aggcgacata ggggggctct tggcgcaaga
     7081 tgcccggaca cgcgcacaag cgcgggcatt tatcggcaca ctgcgctgat gccgtctgaa
     7141 caccgttatc aaaggaaaac catttgcaca cccttctagc ttttatcttc gccatcctga
     7201 ttttggtcag cctgcacgag ttcggacact acatcgttgc cagattgtgc ggcgtcaaag
     7261 tcgtacgctt ttccgtcggc ttcggcaaac cgtttttcac ccgaaagcgc ggcgacaccg
     7321 aatggtgcct cgccccgatt ccgttgggcg gttacgtcaa aatggtcgac acgcgcgaag
     7381 gcgaagtatc agaagccgat ttaccctacg cttttgacaa acaacacccc gccaaacgca
     7441 tcgccatcgt cgccgccggc ccgctgacca acctcgcact ggcggttttg ctgtacggac
     7501 tgagcttttc cttcggcgta accgaactgc gcccctacgt cggcacagtc gaacctgaca
     7561 ccattgccgc ccgcgccggc ttccaaagcg gcgacaaaat acaatccgtc aacggcacac
     7621 ccgttgcaga ttggggcagc gcgcaaaccg aaatcgtcct caacctcgaa gccggcaaag
     7681 tcgccgtcgg cgttcagacg gcatcgggcg cgcaaaccgt ccgcaccatc gatgccgcag
     7741 gcacgccgga agccggtaaa atcgcaaaaa accaaggcta catcggactg atgcccttta
     7801 aaatcacaac cgttgccggc ggcgtggaaa aaggcagccc cgccgaaaaa gcaggcctga
     7861 aaccgggcga caggctgact gccgccgacg gcaaacccat cgcctcatgg caggaatggg
     7921 caaacctgac ccgccaaagc cccggcaaaa aaatcaccct gaactacgaa cgcgccggac
     7981 aaacccatac cgccgacatc cgccccgata ctgtcgaaca gtccgaccac accctgatcg
     8041 ggcgcgtcgg cctccgtccg cagccggaca gggcgtggga cgcgcaaatc cgccgcagct
     8101 accgtccgtc tgttatccgc gcattcggca tgggctggga aaaaaccgtt tcccactcgt
     8161 ggacaaccct caaatttttc ggcaaactaa tcagcggcaa cgcctccgtc agccatattt
     8221 ccggtccgct gaccattgcc gatattgccg gacagtccgc cgaactcggc ttgcaaagtt
     8281 atttggaatt tttggcactg gtcagcatca gcctcggcgt gctgaacctg ctgcccgtcc
     8341 ccgttttgga cggcggccac ctcgtgtttt atactgccga atggatacgc ggcaaacctt
     8401 tgggcgaacg cgtccaaaac atcggtttgc gcttcgggct tgccctcatg atgctgatga
     8461 tggcggtcgc cttcttcaac gacgttaccc ggctgctcgg ttagatttta cgtttcggaa
     8521 tgccgtctga aaccgcattc cgcaccacaa ggaacttacg atgaaactga aacagattgc
     8581 ttccgcactg atgatgttgg gcatatcgcc tttggcattt gccgacttca ccatccaaga
     8641 catccgtgtc gaaggcttgc agcgtaccga gccgagcacc gtattcaact acctgcccgt
     8701 caaagtcggc gatacctaca acgacacaca cggcagtgcc atcatcaaaa gcctgtacgc
     8761 caccggtttc tttgacgacg tacgcgtcga aactgcggac gggcagctcc tgctgaccgt
     8821 tatcgaacgc cccaccatcg gctcgctcaa catcaccggc gcaaaaatgc tgcaaaacga
     8881 cgccattaag aaaaacctcg aatcgttcgg gctggcgcag tcgcaatact ttaatcaggc
     8941 gacactcaat caggcagtcg ccggcctgaa agaagaatac ctcgggcgcg gcaaactcaa
     9001 tatccaaatc acgcccaaag taaccaaact cgcccgcaac cgcgtcgaca tcgacatcac
     9061 gattgacgag ggcaaatccg ccaaaatcac cgacatcgaa tttgaaggca accaagtcta
     9121 ttccgaccgc aaactgatgc ggcagatgtc gctgaccgaa ggcggcattt ggacatggct
     9181 gacacgaagc aaccaattca acgagcagaa atttgcccaa gacatggaaa aagtaaccga
     9241 cttctaccag aacaacggct acttcgattt ccgtatcctc gataccgaca tccaaaccaa
     9301 cgaagacaaa accaagcaga ccatcaaaat caccgtccac gaaggcggac gtttccgttg
     9361 gggcaaagtc tccatcgaag gcgacaccaa cgaagtcccc aaagccgaac tggaaaaact
     9421 gctgaccatg aagcccggca aatggtacga acgccagcag atgaccgccg ttttgggtga
     9481 gattcagaac cgcatgggct cggcaggcta cgcatacagc gaaatcagcg tacagccgct
     9541 gccaaacgcc gaaaccaaaa ccgtcgattt cgtcctgcac atcgaaccgg gccggaaaat
     9601 ctacgtcaac gaaatccaca tcaccggcaa caacaaaacc cgcgacgaag tcgtgcgccg
     9661 cgaattgcgc caaatggaat ccgcgcctta cgacacctcc aagctgcaac gctccaaaga
     9721 gcgcgtcgag cttttgggct acttcgacaa cgtacagttt gatgccgtcc cgcttgccgg
     9781 cacacccgac aaagtcgatt tgaacatgag cctgaccgaa cgttccaccg gctcgctcga
     9841 cttgagcgcg ggctgggtac aggataccgg cctggtcatg tccgcaggcg tttcccaaga
     9901 caacctgttc ggtacgggca agtcggccgc cctgcgcgcc tcacgaagca aaaccacgct
     9961 caacggctcg ctgtcgttta ccgacccgta cttcacggca gacggggtca gcctgggcta
    10021 cgatgtttac ggaaaagcct tcgacccgcg caaagcatcg accagcatca aacaatataa
    10081 aaccaccacg gcaggcgcag gcatccgcat gagcgtgcct gttaccgaat acgaccgcgt
    10141 gaatttcggt ttggtggcag aacacctgac cgtcaacacc tacaacaaag cgcccaaaca
    10201 ctatgccgac tttatcaaga aatacggcaa aaccgacggc acagacggca gcttcaaagg
    10261 ctggctgtac aaaggtaccg tcggctgggg gcgcaacaaa accgacagcg cgttatggcc
    10321 gacgcgcggc tacctgacgg gcgtgaacgc cgaaatcgcc ctgcccggca gcaaactgca
    10381 atactactcc gccacccaca accaaacctg gttcttcccc ttaagcaaaa ccttcacgct
    10441 gatgctcggc ggcgaagtcg gcattgcggg cggctacggc agaaccaaag aaatcccctt
    10501 ctttgaaaac ttctacggcg gcggcctggg ttcggtgcgc ggatacgaaa gcggcacgct
    10561 cggtccgaaa gtgtatgacg aatacggcga aaaaatcagc tacggcggca acaaaaaagc
    10621 caacgtctcc gccgagctgc tcttcccgat gcctggcgcg aaagacgcgc gcaccgtccg
    10681 cctgagcctg tttgccgacg caggcagcgt gtgggacggc aaaacctacg acgacaacag
    10741 cagttccgcg accggcggca gggttcaaaa catttacggc gccggcaata cccataaatc
    10801 cacctttacc aacgaattgc gctattccgc cggcggcgcg gttacctggc tctcgccttt
    10861 aggcccgatg aaattcagct acgcctaccc gctgaagaaa aaaccggaag acgaaatcca
    10921 acgcttccaa ttccaactcg gcacgacgtt ctaatcccgc aaatgccgtc tgaagccctt
    10981 cagacggcat ttcgcggcaa cattcgaagg agttttacca tgacccgttt gacccgcgcg
    11041 tttgccgcgg ctctgatcgg tttgtgctgc accgcaggcg cgcacgccga caccttccaa
    11101 aaaatcggct ttatcaacac cgagcgcatc tacctcgaat ccaagcaggc gcgcaagatt
    11161 caaaaaacgc tggacagcga attttccgcc cgtcaggacg aattgcaaaa actgcaacgc
    11221 gaaggtctgg atttggaaag gcagcttgcc gaaggcaaac tcagaaacgc aaaaaaggcg
    11281 caagccgaag aaaaatggcg cgggctggtc gcagcgttcc gcaaaaaaca ggcgcagttt
    11341 gaagaagact acaacctccg ccgcaacgaa gagtttgcct ccctccagca aaacgccaac
    11401 cgcgtcatcg tcaaaatcgc caaacaggaa ggttacgatg tcattttgca gaacgtgatt
    11461 tacgtcaaca cccaatacga cgttaccgac agcgtcatta aagaaatgaa cgcccgctga
    11521 ccctttcaga cggcataccg aacaggaaaa ccatgattcc ggccacctac accctgtccc
    11581 aaatcaccgc gcggctcggc ggcgaatggc gcggcgagga catttccgtt accgccgtgc
    11641 gcccgctcgc agacgcgcag gcggaacaca tcagcttcct tgccaatccg aaatacaaag
    11701 ccgaagtcca cgacagcagc gcgggcgcgg tcatcgtttc cgccaaagcg gcagacggat
    11761 ttgaagggcg caacctgatt gtcgccgacg acccctatct ctatttcgcc aaagtcgccc
    11821 gcctgttttc acccgtcgtc aaagcgcgcg gcggcatcca tccgaccgcc gtcgtcgaac
    11881 cgagtgccac cgttcccgca agctgcgaaa tcggcgcgaa cgcctacatc ggcgcaaaca
    11941 ccgtgctcgg cgaaggctgc cgcatcttgg caaacgccgt cgtccaacac gattgcaaac
    12001 tgggcgacga agtcgtcctg catcccaacg ccgtcgttta ttacggctgc acactgggca
    12061 gacgcgtcga aatccacagc ggcgcggtca tcggcgcgga cggtttcgga ctcgcttttg
    12121 ccggcgattc atggtttaaa atcccgcaaa ccggcgcggt aacgctgggc gacgacgtag
    12181 aaatcggctc gaacaccaac atcgaccgcg gcgcgatgag cgacaccacc gtcggcaacg
    12241 gcaccaaaat cgacaaccaa gtccaaatcg gacacaactg caaaatcggt tcgcacaccg
    12301 tcatcgccgc caaaaccggc atctcaggca gcgtaaccat aggcagctac tgcatcatcg
    12361 gcggcggcgt cggtacggtc ggacacatcg aaatcgccga caaaaccacc atcggcggcg
    12421 gcacgtccgt cacccacagc attaccgaaa gcggcaaaca cctcgccggc atcttcccga
    12481 tgtccaccca taaagaatgg gcgcgcaacg ctgtttacat ccaccgctta agcgaaatga
    12541 acaaacggct caaaacactg gagcagcagc tttcagatgc cggtcaagac agcaaataac
    12601 caaaccgact ttattcaagg aatacgacag acatggacgt acaactcccc atcgaagcca
    12661 aagacatcca aaaactcatc ccccaccgct atccgtttct ccagctcgac cgcatcaccg
    12721 ccttcgagcc gatgaaaacc ctgaccgcga ttaaaaacgt cagcataaac gagccccagt
    12781 tccaaggcca ctttcccgac ctgcccgtga tgcccggcgt actcatcatc gaagcgatgg
    12841 cgcaagcctg cggcacgttg gcgattttga gcgaaggcgg gcgcaaagaa aacgaattct
    12901 tcttcttcgc cggcatagac gaagcccgtt tcaaacgcca agtcatcccc ggcgaccaac
    12961 tcgtctttga agtcgagctg ctgaccagcc ggcgcggcat cggcaaattc aacgccgttg
    13021 ccaaagtgga cgggcaagtc gccgtcgaag ccatcatcat gtgtgccaaa cgcgtggttt
    13081 gagcgttcgg aaaaaggtcg tctgaaagtt tcagacgacc taataaaaag aaaacccacc
    13141 ggtttaggtt tattcaaacc cagggacacg gtggggatta gttttaccta actgtatcaa
    13201 atatagtttg aaacgtcaat ttaaaaataa agaatcaggt gttagcgtgg attataagga
    13261 atgctgtagt attttttctg atttcagaat ggaacggtat aaaaatgccg ttggggaaga
    13321 caaagcagct gaattgtatt tattgaattt atctttatca agagaacttt ttcatgttgt
    13381 ttcaatattt gagattgttt taagaaacaa aattgacatt tgccttcagc aggcgtttaa
    13441 agacagaaac tggttatata acagcataca gccacagacg aatcccgcat taaaatatca
    13501 aggttgcttt ttaagaaatg gcacgaaaga atctgctgaa ttaatcaagg ttgcattatc
    13561 taaaatacaa aataatagcg ggggaaagtt tgaccacaac caattggttg caggactagg
    13621 attcggtttt tggcggtatc tgtttgcagg agggaaagat gcccaatttg atgctacagg
    13681 gaaagtattg atgaaagttt ttccaaaaaa gcccaaatct acaccaagtg tgcaatataa
    13741 ccaaaaatgg atttttcgag aactttccaa tattaacaaa ttccgtaatc gtttggcaca
    13801 tcacgagccg atttgtttta aaggtgcaat aaaagatacc aactacgcta gaaatattca
    13861 ccaatctatt tttgaactgc ttaattacat ggatgtagat actgcttcag tatttagcca
    13921 ttttagcgat caagtgattg cggtctgcga tgaaatcgac aaactgtaat tttctattat
    13981 ttgtcttaac aagcagcgga gtattcaaaa catgaccctc atccacccga ccgccgtcat
    14041 cgaccccaaa gccgaactcg actccagcgt caaagtcggc gcgtacaccg tcatcggccc
    14101 caacgtccaa atcggcgcga ataccgaaat cggcccgcac gccgtcatca acggccacac
    14161 cagcatcggc gaaaacaacc gcattttcca atttgccagc ctcggcgaaa tcccgcagga
    14221 caaaaaatac cgcgacgagc cgaccaagct gattatcggc aacggcaaca ccatccgcga
    14281 attcaccacc tttaatttag gcacggtaac cggcatcggc gaaacccgta tcggcgacga
    14341 caactggatt atggcgtact gccacctcgc gcacgactgc gtggtcggca accacaccat
    14401 cttcgccaac aacgcctcgc ttgccggaca cgttaccatc ggcgactacg tcgttttggg
    14461 cggctacacg ctggttttcc aattctgccg catcggcgac tacgccatga ccgcgtttgc
    14521 cgcaggcgta cacaaagacg tgccgcccta ctttatggca tcgggctacc gcgccgaacc
    14581 ggcggggctc aacagcgaag gcatgcgccg caacggtttt accgcagagc agatttccgc
    14641 cgtcaaagac gtgtacaaaa ccctctacca tcgcggcatt ccgtttgaag aagccaaggc
    14701 agacattctc cgccacgccg aaacccaagc cgagcttgcc gtattccgag actttttcgc
    14761 acaatcggca cgcggcatca tccgctgacc gtaccctttt gatgccgtct gaaaccccga
    14821 cagcgtttca gacggcatcg gtttttttaa agcggtatat tgtttgatcg atgcatcatt
    14881 ttgtagattt cagaagtatg gcagtcggca gcattgatga tttctgccgc ccgccacatc
    14941 cccattcgga catttcattg tgacctccgc cgctgctttt aaagaattgc aaacaatttc
    15001 cgcagcttga accggcctgt tttcggcgaa aaaaaccaaa agtatttttc aaaagaacat
    15061 attgtcttat tggatattct gtgctgaaat tactggacac tacaaaagtg gtgttttaaa
    15121 aataaaacag acaaaggaga aagccatgca agtgttttta gacaatccca aagccttttt
    15181 tgaaaccgtc agcggctggg tttggggacc tgttatgttg atgctgctgg tcggcacggg
    15241 tattttgctg actgttttat taaaaggttt gcagttcacg atgttgggtt atgcgctgaa
    15301 acaggcgttt gtgccgccaa agaagcataa aagcggcgaa ggccacgaag gcgatatttc
    15361 ccatttcgcg gcgttgatga ccgcgctgtc cgccaccatc ggtacgggta acatcgccgg
    15421 cgtggcgact gcggtggtaa ccggcggccc gggcgcggta ttttggatgt ggatgaccgc
    15481 cattttcggc atggccacca aatacggcga aggcgtgttg gcggtgaaat accgcgtcaa
    15541 caattccaaa ggcgaaatgt ccggcggccc gatgtattac atcgaaaaag gcttgggcaa
    15601 aaactggaaa tggatggcgt tggcgttcgc tttattcggc acattcgctt ccttcggtat
    15661 cggcagctcg gtgcagtcca actcggttgc acaggcggtg caaaccagct tcggtatcga
    15721 acctgcctat accggcatta cgttgaccgt tctgactgcc gttgtcgttt taggtggtat
    15781 taaaggcatc gccaaagccg cttctttcat cgtgcctgct atggcggtgt tttatgtgtt
    15841 gggcggtctt tccattatcg cgattaattc cgatgcactg atgcctgccg tcaagctaat
    15901 tttctccgat gcgttcagcg cgcaggcagt ggcgggcggc gctatcggta cggtcatccg
    15961 ctacggcgtg gcgcgcggcg tgttctccaa tgaggcgggt atgggttctg cgcctattgc
    16021 cgccgcagcc gcgaaaaccg accatcccgt ccgtcaggcc ttggtttcta tgaccggtac
    16081 gtttttggat accatcgtcg tttgctcgat taccggcatc gtgttggtaa tgggactgct
    16141 cggcgcgggc ggcgagtttg tcaaacctga agtgagcggc gcggcactga caaccgtcac
    16201 attccaaaaa atgctgcccg gcatcggcgg ctggattgtt accatcggcc tgattttctt
    16261 tgcctactca accattttgg gctggtgcta ttacggcgaa aaatgcgcgg tgtatgtttt
    16321 cggcgagaaa tttgccggtt tgtaccgcgt gggttatgtt tcttccgtta tgctgggtac
    16381 cgtgttgagc cttgatttgg tgtggctggc ttcggacaca ttcaacggct tgatggcatt
    16441 gcccaacctg attgcacttt tattgatggc gaaagtcatc gtcaatgaaa cgcgcgactt
    16501 caaacaaaaa atcacaaacg gcgaattgcc gcattaatat ccgaggccgt ctgaatccga
    16561 atattcagac ggcacggcat attaagtctg ttttgaaaaa gtcggaggga gaaacatgaa
    16621 agtgcttgtt ttaggtgcgg gtgttgccgg cgtgtcttcc gcgtggtatc tggcagaggc
    16681 cggacatgaa gtaacggtca tcgaccgcgc caagggtgtg gcgatggaaa ccagttttgc
    16741 caatgcaggc cagctttctt acggctatac cacgccttgg gctgcacccg gtattccgac
    16801 caaagcactg aaatggctgt ttaaaagcca tccgcctttg ctgtttcgcc ccgacggcag
    16861 cctgtatcaa atcgaatggc tgtggcggat gctgcaaaac tgcacggcag cgcactatca
    16921 aaccaataaa gagcgcatgg tcaggatttc cgaatatagc cgtgaaatgt tccgccgttt
    16981 tgaagcgcaa accggcatga attttgagga acgcaaaaaa gggacgttgc agattttccg
    17041 ccaaaccaaa gaagtcgaag cggcagaaca agacattgcc gttttggaac gctacggcgt
    17101 gccgtaccgc cgtctgaaac ccgaagaatg tgcagaattt gagcctgcgc tggcacgcgt
    17161 taccgccaaa attgccggcg gcctgcacct gcctgcagat gcgaccggcg actgccacct
    17221 cttcactgaa aacctataca aattgtgtca ggaaaagggc gtacagtttc atttcaacca
    17281 aaccatcagc cgcatcgacc ataacgggct gcgcatcaaa gccgttgaaa ccgaaacagg
    17341 gcggtttgaa gcagatgccg ttgtctgtgc gctcggctgc ttcagtagga ctgtgttggc
    17401 gcagttagac ctcgatctgc cgatttatcc cgtcaaaggc tattccttga ccctgccggt
    17461 caccaattca gacggcgcgc cggtgtccac cgttttggat gaaagctaca aagtcgccat
    17521 cacgcgcttt gacaacagaa tccgcgtcgg cggcatggca gaattgtcgg gctacgaaat
    17581 caaactgccc gaaaaacgcc gcgaaacctt ggctttggtc gtcaacgatt tgttcccgga
    17641 aggcggcgat ttgagccaag cattgttctg gagcggcctc agaccgatga cgcccgacag
    17701 cacgccgtta atcggccgca cccgctttga aaacttattc ttgaatacag gccacggcac
    17761 tttgggctgg accatgtcgc tgggttcggc aaaattaacc gccgatatcg tgagcggcaa
    17821 agacaccgaa atccgaagcg acgatttgag cctgtcgcgc tatcaggctt aagctggaaa
    17881 gcgataaggt ttagcgacat ttcaaattta aaggccgtct gaaaatcatt tttcagacgg
    17941 ccttttgttt caaaattcgg gtatccaatc aataaaataa ataataacta ttatcatacg
    18001 cattaaaaag tgttagtctg aacctgtccc agcttttagc gcaggttttc ccatgcccga
    18061 tttttcgatg tccaatttgg ccgttgcctt ttccattacg ttggctgccg gtttgtttac
    18121 cgtattaggc agcggcttgg tgatgttttc caaaacgccc aatccgcgcg tgttgtcgtt
    18181 tggtttggca tttgccggcg gtgcgatggt gtatgtttcc ctgacggaga ttttcagtaa
    18241 gtccagcgag gcgttcgctg aaatttatga taaagaccac gcgtttgcgg cggcgaccat
    18301 ggcatttttg gcagggatgg gcggcattgc gctgattgac cgtctggtgc cgaacccgca
    18361 tgaaacttta gacgcgcaag acccgtcgtt tcaagaaagc aaacgccgcc atatcgcgcg
    18421 agtcggcatg atggcggcgt ttgcgattac tgcgcacaat ttccccgaag gcttggcgac
    18481 gttttttgcc acattggaaa atccagcagt cgggatgcct ttggccttgg cgattgccat
    18541 ccataatatt ccggagggca tttccatcgc cgcgccggtt tattttgcca cccgcagccg
    18601 taagaaaacg gtgtgggcgt gtctgctatc cggcttggcc gagccgttgg gggcggcttt
    18661 gggctatttg gttttgcagc cgtttttgtc gcctgccgtg tttggttcgg tattcggcgt
    18721 gatagccggt gtgatggtgt ttttggcgtt ggacgagctg ctgccggctg ccaaacgcta
    18781 ttcagacggc catgaaaccg tttacggcct gacaatgggc atggcggtga ttgccgtcag
    18841 cctggtattg ttccattttt aaaacctgta tgccaaaggc cgtctgaaat gattcagacg
    18901 gcctttttgc gttataatcc gcacatcaaa aataacctta atttcacaaa gttccatcat
    18961 gttagacaaa tactcccccg ccgaaatcga atccaaacat tatcaaaact gggaagagca
    19021 gggctatttc caacctgata tggatttgac caaaccgtct ttttccatcc aactgccgcc
    19081 gcccaatgta accggcacgc tgcacatggg tcatgccttc aaccaaacca ttatggacgg
    19141 cctgacccgc tactaccgta tgaaaggctg caacaccgcc tggattcccg gcaccgacca
    19201 cgcgggcatc gccacgcaaa tcgtggtcga gcgtcagctt gccgcgcaaa acgtgtcccg
    19261 tcatgacttg ggtcgtgaaa aattcttgga aaaagtgtgg gaatggaaag aagtttccgg
    19321 cggcacgatt acgcagcaga tgcgccgtgt gggctgttct gccgactgga cgcgcgagta
    19381 tttcacaatg gacgatgtac gcgccgaaac cgtaaccgaa gtgttcgtac gcctgtatga
    19441 acagggcttg atttatcgcg gcaaacgctt ggtgaactgg gatccggtat tgggtaccgc
    19501 agtgtctgat ttggaagtgg aaagcgtgga agaacaaggc tctatgtggc acatccgcta
    19561 tccgctggcg gacaatcctg ccgaagccgt catcgtcgcc accacccgtc ccgaaacgct
    19621 gttgggcgac gttgccgttg ccgtcaatcc cgaagacgaa cgttataccc acttaatcgg
    19681 caaagaatta atcctgccgc tgaccggccg caccattccc gtgattgccg acgaatacgt
    19741 tgaaaaagac ttcggcaccg gctgcgtgaa aatcacgcct gcgcacgact tcaacgacta
    19801 cgaagtcggc aaacgccacg atacgcgctt ggttaatgtg ttcgacttgg aagccaaagt
    19861 attggcaaac gccgaagtgt tcaacttcaa aggcgaagcg caaccaagct ttgccctgcc
    19921 tgaaaaatac gcaggcttgg accgctttgc cgcgcgcaaa caaatggttg ccgatttgca
    19981 ggaacaaggt ttcttggtcg aaatcaaagc gcacacgctg atgacgccga aaggcgaccg
    20041 taccggttcc gtgattgagc cgatgctgac cagccaatgg tttgtcgcca tgtccgccac
    20101 gccaaacggc ggcgagcctg acagcgaatt caaaggctta agccttgctg acaaagccaa
    20161 aaaagccgtc gacagcggcg cggtgcgctt tatccccgaa aactgggtca acacctacaa
    20221 ccaatggatg aacaacatcc aagactggtg catctcgcgc caactgtggt gggggcatca
    20281 aatccctgca tggtacgaca acgaaggcaa tgtgtacgtt gcccgcaatc aggaagaagc
    20341 cgaaaaacaa gccggcaaaa caggcttgac ccgagaagaa gacgtattgg acacatggtt
    20401 ctcctccgca ctcgtgccgt tctccacgct cggctggccg tctgaaaccg acgaactcaa
    20461 agccttcctg ccgtccaacg tcttggttac cggctacgaa atcatcttct tctgggtggc
    20521 gcgcatgatt atgatgacca cccactttac cggcaaagta ccgtttaaag acgtgtacat
    20581 ccacggcatc gtgcgcgacc acgaaggcaa aaaaatgtcc aaatccgaag gcaacgtcat
    20641 tgaccctgtg gatttgatcg acggcatcga cttggaaaaa ctgctggtga aacgtaccac
    20701 cggtctgcgc aaacccgaaa ccgcgccgaa agtggaagaa gcctcgagaa aactcttccc
    20761 cgaaggcatt ccgagtatgg gcgcggacgc tttgcgcttc accatggcga gctacgccag
    20821 cttggggcgc agcgtgaact tcgacttcaa acgcgccgaa ggctaccgca acttctgcaa
    20881 caaaatctgg aacgccacca acttcgtctt gatgaacacc gaaaaccaag actgcggcta
    20941 cggcgcaacc gccactgagc cgcgcggcta ttccttcccc gatatgtgga ttgtagaccg
    21001 tctgaatcag accatcgagc aagtaacgca agcctacgaa acctaccgct ttgatttggc
    21061 agcggaaacc ctgtacagct tcatgtggaa cgactattgc gactggtatt tggaacttgc
    21121 caaagtgcag cttcaaaccg gctgcgccag ccgccaacgc gccacacgcc ataccttgtt
    21181 gcgcgtactc gaagccgccc tgcgcctgct gcacccgatt atcccgttca tcaccgaaga
    21241 attgtggcaa accgtcgctc ctatgtgcga cgcgaaaacc gccgacagca tcatgctcgc
    21301 ccgcttcccc gaagccgacg gcggcgatat tgttcagacg gcattcgagc aaatgaccgt
    21361 gttgcaggat ttgattggcg cagtccgcaa cctgcgcggc gaaatgggca tccagcccaa
    21421 tgtgaaagcc ccgctgtttg tcgaaagcac ggacgacttg gcggactacc tcaaatacct
    21481 gccgatgatg acccgcctga tcgaagcaca gcaagtcgcc gccctacccg aaagcgaaga
    21541 cgcgcccgtc gccgtctgca acggcgcgcg cctgatgttg aaagtcgaaa tcgacaaagc
    21601 tgcggaaacc gcccgtttga gcaaagaagc cgagaagctg caaaaagcct tggacaaact
    21661 caacgccaaa ctctccaaac ccggctacac cgaaaaagcc cctgcgcatc tggtggaaaa
    21721 agacaaagcc gatttggcgg aattggaaga caaaatggca aaagttcaaa atcagttggc
    21781 gaagttgaaa gactgatttg attgaataaa caaggccgtc tgaaaaatgt ttttcagacg
    21841 gcctctttta tagtggatta acaaaaatca ggacaaggcg acgaagccgc agacagtata
    21901 aatagtacgg caaggcgagg caacgccgta ctggtttttg ttaatccact atacatggca
    21961 ttgtatcggc actgatgaaa acattcacag tgatgcaaaa gtcgaatatc ttcaggacgg
    22021 cagtttccgt ggcgatgcta tactcaaaat cgatgatgac ggcaatatct tggcataccg
    22081 tgttgtcggc tcagggaaat ggcgttttga aaacaatgcc tgcgcctagt cgcggataaa
    22141 acttaccccg tgaagctgcg actgcataat cgccgccttc atcgccgaca acgccttcgg
    22201 acggacaaaa ttgcggcggt acgccaatac gacccgtcgg cttggcggcg taccctcaaa
    22261 cggaataatg ctgaacagca tatgatcgtt ctcggtcagt gcggttgccg gcaacacgct
    22321 gattgccaaa ccgctggcga ccatatggcg gattgtattg atcgagctgc cctgcaatgt
    22381 attggtcaac ccctgtatgc gttgtttcgc cgccaattcg gaacagcttg agagtacctg
    22441 atcccgcata cagttgcctt ccgtcagcag caaaacctgc tcctcaccca gcatccgggg
    22501 cgaaacggca tccagttcct caaatgaatg ccctttcggg acaatcacga aaaacggttc
    22561 gtcatacaag ggttcggtaa caatgcccgg ctcttgaaac ggttcggcaa cgataatcgc
    22621 atcaacgtcc ccgcgtttga gcgactcggt caaagtatgc gtgtaattct cttccaacat
    22681 caaaggcatt ttcggtgcag tacggcgcaa cgagacaatc agtttcggca gcaggtacgg
    22741 cgcaaccgta aaaatcagcc cgagtttgaa cgcaccctcc agctcgtttt gttcttcatt
    22801 tgccaaatgc ctgataagct ctgcctcttc caacacctta cgcgcctgtg caacgatacg
    22861 ttcccccgcc tcggtcgtaa taatatcgtt gctgctccgg tcaaacaaag agacggcaag
    22921 ctcttcttcc aatttcttaa tggcaataga caaagtgggc tgactgacaa aacaacgccg
    22981 cgccgccctg ccgaaatgac gttcttgggc gactgcgacg atgtaccgca attcggttaa
    23041 ggtcatgtcc tatacctttt tctgttccgg caaagtaatg ttcaactcaa gcacatccat
    23101 accatcctgt ttttcctggg aaatacggat attgtctaat gaaacattca catatttgga
    23161 caggacttcc atcaactctt tacgtaaagt cggcaggtaa tccggagcct gaccttcttg
    23221 ggcgcgctct tgggcaatga tgatttgaag gcggtcgcgg gcaacggttg ccgttttctg
    23281 ctttctgccg aataaaaatt cgattaatga catatcttat cctccaaaca gacgtttgaa
    23341 gaagcttttt ttctcagctt ccaagaaacg catttcacgg ttctcgccca aaagacgggc
    23401 aataacgtcc ttatatgcct cggaagccgc cacgctgtcc tgatggatga ccggttctcc
    23461 ggaattggat gcctgcaaga cgttttggga ttcaggaatc acacccagca aaggaatatg
    23521 cagaatatcg caaatatcct gtacagacag catttcgcct tttgccacac gttcgggaga
    23581 ataacgcgta atcaacagat gttctttaac cgaaccgcct tgctctgcct tatgggattt
    23641 gctttgcaaa attcccaaaa tcctgtcgga gtcacgcaca ctggaaacct caggattggt
    23701 cgttacaatg gcttcatcag caaaatacaa cgccatcaat gcaccctgct caataccggc
    23761 aggagagtcg caaataatat actcaaagcc cattttcttg ccggacagct cctgcatcac
    23821 tttttctacg ccctcgcgtg tcaaagcgtc tttatcccga gtctgggaag ccggcaaaat
    23881 aaacaggttt tcacaatttt tatctttaat caaagcttgg ttgagcgtcg cctccccctg
    23941 aatgacattg atcaggtcat aaacgacacg acgctcgcaa cccataatga ggtcgaggtt
    24001 acgcaaaccc acatcaaaat caattaccgc agttttatat ccgcgtaatg ccaaacctgt
    24061 cgcaatactg gcactggtag tcgttttacc gacaccgccc ttacctgaag ttactacaat
    24121 aatttttgcc acaatatttc ctttttaaat atcaaacaat tactctgagc caattgcact
    24181 gataaccaat cggttatcct gcaacaatat ctgtaccggc tgcttgtgca gatggttcgg
    24241 caaatcctgt tcaaaattac ggtaaatacc cgccacagaa accagttctg cctgcatgga
    24301 gtggataaat atgcgggcag aagtatcacc cttggcaccg gccaaagcac gccccctcat
    24361 cggcgcataa atatgtatat tgccatccgc aatcaattcc gccccctggc tgaccgcccc
    24421 cgtaacaatc aaatcgccat cttcggcata aacctgctga ccggtacgga caggggatgt
    24481 aatcaatact gttttccttg ctttctgacc atcctccgtg ttctgcacct caacctgacc
    24541 cagttcttta acattttccg aatgagacag acaaaacagc aaatgatact tcatagccgc
    24601 agcagcccaa cgttcattag aatgcttcag acccaaaatt tgcataccat gccttgaaaa
    24661 caacgaaacc aatgcagcaa gatccaaaga ctcgggataa tcaaactctt gaacatccag
    24721 cacaaatgga acaacaccag actcttgaaa cttcttgccc aatttgacca gcacatcttc
    24781 caaatcaaac aagtctgacg tatgcaaaga tatagacaat acgtccatct ttgtcgactt
    24841 tatatcgaaa gcattcatta tataaaccat catagaattt tttaatgatg taagtttacc
    24901 gcgcaaacct tagctattca attaatattt taaacatgat agccaatacc ctattaccta
    24961 tcagatattg caaaaataac gccacctgct tttcggcaga tggcgttata tgactaaaat
    25021 tactcagcag ccggtttgtc aaccaattca accagtgcca gaggtgcatt atcaccttta
    25081 cggaatccgt atttcaacac ccgaacataa ccaccgttac gagcagtaaa acgaggaccc
    25141 aaatcgccaa acagttttac tacaacatca cggtcgcgag tacggtcaaa tgccaaacgg
    25201 cggtttgcca atgacggctt tttacccaat gtaatcaacg gctctactac acggcgcaat
    25261 tccttggcct taggcagagt tgttacaata gtttcgtgag tcaataatga attcgccata
    25321 ttacgcagca ttgcagcacg atgactgctg gtacggttta atttgcgatt gccattacga
    25381 tgacgcatat caattatcct ttaattcttc attaaggctt ttccaagcct acaggtggcc
    25441 atgcttccaa cttagaaccc agtgtcaaac ctttagatgc caatacttcc ttaatctcat
    25501 tcaaagattt acgtcccaaa ttcggcgttt taagaagctc ggtttcagtg cgttgaatca
    25561 aatcgccaat ataataaata tcctcagctt tcaaacaatt agctgaacgt actgtcaatt
    25621 ccagatcatc caccggacgc aaaagaacag ggtcgatagg aggtgctttt tcttcaacct
    25681 cctccacagg cgtaccctgc aaatcagcaa aaatagacat ctgatcaatc aaaatacgtg
    25741 ccgcactgcg tacagcttcc tcaggatcaa tagaaccgtc ggtttcgata tccaaaacca
    25801 acttatcaag atccgtccgc tgctctacac gtgcaggttc aacctcaaag ctaacacggc
    25861 tgatgggcga aaagctcgca tccaactgga ttgcaccaat ctgacggttc tcatcacgaa
    25921 ctacctgacg acctgaaaca gattgataac cacgaccttg ctctacttta atttccatct
    25981 caatttgacc gttatcagcc aaatgacaaa tgacatgacc aggattcaga atttctacat
    26041 catgcggcaa ctcaatatca ccggcagata cgacacctga acctgatttc ttcaacacaa
    26101 gttgaacttg gctacgacca tggagtttaa acacaatacc tttaatattc agcaaaatgt
    26161 caacaacatc ttcctgaata ccatcaacag tagaatattc gtgtaataca ccggcaatag
    26221 ctacttcagt aggagcaaaa ccattcatgg atgacagtaa gatacggcgc aaagcattac
    26281 ctaaggtatg accgaaacca cgttcaaatg gctgcataga tacttttgca cgagttgcag
    26341 aaaaagtatt tacatcaatt tgacgaggtt tcaaaaattc ggttgtgcta ttctgcattt
    26401 aactgtccct cactgagcta gcattattta gagtagaact ctaccaccag ctgttcatta
    26461 atatcaccgg tcaattccga gcgatccggc atgtttttga acacaccctc aagtttatcc
    26521 gcatctacag aaacccaacc cggcaagccg atttgagttg ccaaacccaa tgcttcttga
    26581 atacgtacct gttttttggc tttttcacga actgagacaa catcaccagc tttcacttgg
    26641 aaagaaggaa tattgacaac ttgtccattc acaactatcg ccttatgaga aacaagctgt
    26701 cttgcttcag ctcgggtaga accgaaaccc atacgataaa cgacattatc caaacgagat
    26761 tccagcaact gcaacagcaa ctcgccggta gaacctttac gacgatcagc ttctgcgaaa
    26821 taacgacgga actgacgttc taatacgcca taaatacggc ggattttttg tttttcacgc
    26881 aactgcaaac catagtctga caaacgcggt ttttttgcac catgctgacc aggagcggaa
    26941 tcaattttac atttagaatc caaagagcgg cgcgcactct tcaaaaacaa atccgtacct
    27001 tcgcgacgtg ccaacttaca tttagggcca atataacgtg ccatgtttca aatcactcca
    27061 atattaaata cgacgttttt taggcggacg gcaaccgtta tgaggcaacg gggtaacgtc
    27121 agtaatgctg gtaatcttga aaccaagagc attcaaagca cgtacagagg attcgcgacc
    27181 tggacctgga cctttaatac gaacctctaa atttttaacg ccatactctt gggcaacttt
    27241 accagctgct tctgctgcaa cttgtgctgc aaatggtgta cttttacgag aacctttaaa
    27301 accagcgccg ccagaggtag cccaagacaa cgcattgcct tgacggtcag tgattgtaat
    27361 gatggtattg ttgaaagatg catgaacgtg cacaataccc tcactcacgg ttttacgtac
    27421 ttttttacgt acacgtgaag ctgtgtttgc tttagccatt aataaaattc cttaaaattt
    27481 atttcttacc agcaatcgct ttacgcggac ctttgcgggt acgcgcattt gtacgagtgc
    27541 gttgaccgcg gcatggtaag ccgcgacgat gacggaagcc acgatagcag cccatgtcca
    27601 tcaatcgctt gatactcata gttacctcac gacgcaaatc accttctact tcatacttgg
    27661 caacttggtc acgcaaagca tctaattgag tctcgtccaa atcttttgct ttagtatcag
    27721 gcgcaatatt tgcagcctca caaatcaatt tagcacgagt agcaccaata ccgtaaatag
    27781 cctgaagacc aattacgatg tgtgcgttat tagggatatt tacccctgca atacgagcca
    27841 tattttttcc tttttagggc aaagtgtgtc actatatcac agaatcacat taaaagaaat
    27901 attccattaa ccttgacgct gtttgtgacg gagatcagta caaattacac gaactacacg
    27961 atttcgacga ataatcttgc aatttcggca aattttctta acagatggtt gtacacgcat
    28021 tttattttcc ttttattggt ttatcttgct ctgaaaacga ttcgagccct agttagatca
    28081 taaggtgtca gctctactgt gaccttatct cccggagaaa tacgaatgta atgcatccgc
    28141 atcttcccag aaatatgacc caatacaata tggtcattct caagttttac tttaaatgtt
    28201 gcattaggta aagtttcaag aatttcacct tgcatttgga tagtatcttc tttcgccata
    28261 attctatttc cgagaaaatg atttcatttc tgaacgagtc attaacttat catactgttg
    28321 agtaagccta tacgaattta tttgtgtact aaaatccatc gttacaacaa ctagaatcaa
    28381 caaagacgtg ccacccaaat aaaaaggtac atttaaaacc gtagttaaga actctggaat
    28441 taaacaaata gttgtaatat aaagagctcc aaacaatgtc aaacgtaata caactttttc
    28501 taaataccta gaggtctgct caccaggtct aatcccagga acaaaagcac cactcttttt
    28561 taaattctct gccatttctt tagggctaaa aaccaaagcc gtataaaaat aacaaaagaa
    28621 aataactgtc gctgcaaata aagccatata cagcaattga ccgtgttgta acaatccagc
    28681 tattttgtgc aaaacactat ttgtatcagc cgaaccaaac caacctaaaa gagtagatgg
    28741 aaatagaata atactggaag caaaaattgg gggaataaca ccagccatat tcaacttgaa
    28801 aggcatatgc gtattttgac tacccgccct accattaaac tggcgttttg cataatgaat
    28861 aggaatcttc cgctgtgcac tttcaaagta tacaaccaaa taaattaata ataaggcacc
    28921 aaatacaata aacaacgccg taagcatgct catagaacct tggttcgtca gtgtaaccag
    28981 ctttgcaata cccgaaggaa tacctgaagc aatacctgcc gtaatgatta aagaaatacc
    29041 gttcccgata cccctttcag taatttgctc cccaagccac ataagaaaca tggttcccgt
    29101 taccaaagaa actaccgtgg aaacatgaaa ctcaaatgaa cttgttacaa caattccttg
    29161 ctgaaatacg aaagatgcaa cacctagact ttgaagaatt gctaacaaaa cagtaccata
    29221 cctagtatat ttcgtaatta cctttctacc agcctcccct tcttttttta aagccttcaa
    29281 tgatggcaaa atttcagaag cgagctgtac aataatagaa gctgaaatat atggcataat
    29341 tcctattgca aatatactaa agcgctctaa cgacccaccg gaaaacatat tcaatattcc
    29401 caggatgccg tttccagcgc tttcgtataa tttagctaaa gcaacagcat caactccagg
    29461 tacgggtata tgggcaccaa ttcgaaaaac aatcaatgct ccaaatagaa acaaaagacg
    29521 tttcttaaga tctccaaatt tggatgaacc tgatgacgtt tgttgattag ccacaatatt
    29581 aaaccttaca tttcaatctt accaccaaca gcctcgatag cagctctcgc acctttggta
    29641 actttaatac ccttcaaagc aactgcctta gaaatttcac cagaagcaat aactttaaca
    29701 ttagagactg tagatgcaat cagacccgct tgctttaaga ccaaaatatc aatctcatta
    29761 acagcaattg attccagttc acttaaacga agctgtgcat tagctgatgc tgttaaagat
    29821 ttaaaacctc ttttagggag gcgtcgttgc aagggcattt gaccaccctc gaaacccacc
    29881 ttatgaaacc caccagaccg actcttttga cctttatgac cacgaccacc cgttttgcca
    29941 agaccactac caataccgcg tccaacacga cgaccagcat gcgtagcacc aacagcaggt
    30001 tgaattgtat tcaaaaacat atcaagactc cactttcaac aagtagctga ttttattaat
    30061 cataccacgg ttttcagggg tatctaaaac ctctaccgta tgctcgcgac gacgcaaacc
    30121 taaaccgcgt gcacatgcac gatgagattc aattgtacca atcaggcttt taaccaatgt
    30181 aaccctaatc tttttttgtt cagccatggt taactcccaa aatgtcttcc actgtcaagc
    30241 cacgtttggc tgcgatatca gcaggagtat gcaacttaga caaaccatct aatgttgcac
    30301 gtacgatatt atatgggtta gtagatccgt gcactttggc ggagatatta tgaatgccca
    30361 tagcatcaaa aaccaaacgc ataggtccac cggcttttac gccactaccc tctttagcag
    30421 gctgcataaa tactttagta gcaccatgtc gaccaataac ctcatgatga atcgtaccat
    30481 tttttaatgg taccttaatc atagagcgtc gtgcttgatc catcgccttt tggactgcaa
    30541 caggcacttc ttttgactta cctttaccca taccaatacg accatctcca tcaccaacaa
    30601 cagttagcgc agagaaagcc ataatgcgac cacctttaac tactttggtt acacggttaa
    30661 ctgcgaccat cttttcaatc agaccgtcac cgcgttcttc aatttcatgt tttgccatct
    30721 gaaagtctcc aaatatttag aagcttaaac cattttcacg agcagcttca gccaaagcct
    30781 tcacacgacc gtgatattgg aaacctgaac gatcaaaagc aaccttttct acacctgctg
    30841 ctttagcctt ctcagcgata cgtttaccaa ctattgcagc tgcttcaaca ttgcttccag
    30901 atttcagact accgcgcacc tcagcttcca atgtagaggc ttgagccaat actttatcac
    30961 cttcagcact aattacttga gcataaatat gattattgct tcggaacaca cataatctta
    31021 ccattttcaa gtccgcaata cgagcacggg ttttgcgtgc acgacggagt cgggttgtat
    31081 gtttatccat tagtgaacct caattatttt ttcttggctt ctttcattac cactacttct
    31141 cctacatagc gaacaccttt acctttataa ggctcaggag cacggaacgc acgaatctca
    31201 gcagcaactt gaccaacaac ttgtttatcc gagccggtta aaacaatctc tgtttggcta
    31261 ggagtttgaa cggagacacc ttcaggcatt tcatatacga tcggatgaga aaaacccaaa
    31321 gacagattca agattttacc ttgtgcttga gcacgataac ccacacctat caattgcaat
    31381 cttttctcaa aaccttctga aacaccttta accatattgc tgactaatgc gcgagcagta
    31441 ccagacattg catttgcttg tttactgctg ttattcgcaa caaaagccaa tttgccatca
    31501 ttaaattcaa tggctacatc agaatgcaaa ggaaaagaca attcaccgtt cttaccctta
    31561 ataactaatg cctctgttcc aaattttact tctacaccag cgggaacagt cactgggttt
    31621 tttgcgacac gtgacatttc ttttcctcca ctaggctaca atgcataaca actcaccacc
    31681 aacaccttga gaacgtgctt tacgatcagt cattacacct ttagaagtac taacaatagc
    31741 aatacccaag ccattcataa cacttggaat ctcactagac gctttataaa tacgcaaacc
    31801 ggggcgagat acacgcttga tttgttcaat tacaggacga cctgcatagt attttaattg
    31861 aatttccaat atagacttta cgtcagatga aactgcgaag tcctcaatat atccttcttc
    31921 tttcaatacc tttgcaatag cacactttaa ttttgaagaa ggcattgcaa ccgctgcttt
    31981 attagcacgt tgcgcattgc ggatacgagt caacatatcg gaaataggat catgcatact
    32041 cattcttaat tactcctatt accagctggc tttaacaaca cccggaattt cgccacgcat
    32101 ggcgatttca cggattttaa tacgacccaa accaaattta cggaaagtac cacgagggcg
    32161 acctgtcaaa gcacaacgac gacgttgacg cacaggtgcc gcattacgag gaatggattg
    32221 aaacctcaaa cgagcctcaa aacgctcttc ctcagttgca ttcgaatcat tgattaccgc
    32281 tttaattgcc gcgcgtttag ccgcatattt tttagccaaa gcttgacgtt tcagattacg
    32341 attaataagt gctttcttag ccatgattat cctttgaacg gaaatttaaa caatgacaat
    32401 aaagctttcg cttcctcatc ggtttttgct gtagtagtaa tagtaatatt caaaccacgc
    32461 aaagcatcaa ttttatcgta ttcaatttcc ggaaaaataa tttgctcacg aacacccata
    32521 ttgtaattgc cacggccatc aaatgattta ccgctcacac cacggaagtc acgtacgcga
    32581 ggtaatgcaa tagtaatcaa acgatccaag aattcaaaca tttgatcacg acgcaatgtt
    32641 actttgcaac caaccggata gttatcacgg attttaaaac ctgcgataga tttacgggca
    32701 acagtaacaa ccggtttttg accggcaatt ttctctaaat cggaaacagc atgttccata
    32761 acttttttgt cagcaacagc ctcacccaca cccatattca aggtgatttt ttcaatacgc
    32821 gggacttcca ttactgattt gtaaccaaat tgtttaacca attcaggaac aactgtctct
    32881 ttataaaact ctctcaaccg agccatgtta tctccttatg ccccaatgat agagccattt
    32941 gatttgaaga aacgaacgcg tttaactttg ccttcatttt caatcagctt aataccaaca
    33001 cggtccgctt tattagtttc cggattcagg attgcgatat tagaaatatc caaaggcatt
    33061 tctttagtaa taataccgcc ctcaatgcca cgcattggat taggtttttg atggcgtttt
    33121 acaacattaa cgccctcaac aacaacttta tcacccaaca ctcgaactac ttgaccctgc
    33181 ttacctttat ccttaccagc aattactaca accctatcgc ctttaatgat tttattcatc
    33241 gtgccattcc ttataatact tcaggtgcca atgaaacgat tttcataaat cgctcagtac
    33301 gcaattcacg ggttaccgga ccaaagatac gagtacccaa aggttcaagt ttattattca
    33361 gtaacacggc ggcattgtta tcgaatttaa ttaacgcacc atcaggacga cgtacaccct
    33421 tagcagtacg aacaactacc gcattatata catcgccttt tttgacacgg ccacgcggag
    33481 ccgcatcttt aactgccact ttaataatat cgccaacaga agcgtagcga cgcttagatc
    33541 cgcccaatac cttgatacac attacgcgac gcgcaccaga gttatcagcc acatctaaga
    33601 tggtctgcat ttgaatcatg ttattacctt taaattaacc aacttaattt accactttca
    33661 gataacttac agcaaagccg ctgcttatta aatgaaacct tgcggttcaa gtaaaccagt
    33721 cttggacccc gaaagggaag aaacttccag aagaaactga aagacaggaa acgaagttta
    33781 cacgcaaatt aaatttgctg caatacttcg tttcccattc caagcacgtt gctttaattc
    33841 ttaaatagaa cgtgctttct caaccagctc actgacaacc caagatttag tttttgacaa
    33901 tggacgggat tccgatataa caaccacatc accaattcca tattgattat tttcatcatg
    33961 ggcatggatt ttagtagata accgaataat cttaccatac agcggatgtt ttactttacg
    34021 ctcaaccaat actgttacgg ttttatccat tttgtcgctt actactttgc cttgcaaagt
    34081 acgaacattt ttagtttcgc tcattactta gcaccttttt cagttaaaac ggttttaata
    34141 cgagcaatat cgcgacgtac acgtttcaat tcacttggtt tgcctaattg accggtagcg
    34201 ttttgcatac gtaagccaaa ctgagctttc aacaagtcca acaaatctgc attcaactgc
    34261 tcaacggatt tgtctttcaa ttcatttgct ttcattattg acccacctgt cttactacaa
    34321 aggttgtagg aataggcaat ttggcagcag ccaactcgaa tgcttcacga gccagttcct
    34381 ctggaacgcc atccatttca tacaacactt tacctggttt aatttcggca atgtaatatt
    34441 ccacgttacc tttaccgcca cccatacgaa cttgaatagg cttttcagta atcggtttat
    34501 cagggaatac acgaatccaa atacgaccac cacgtttgat atgacgggtc attgcacgac
    34561 gagcagcttc gatttgacgg gcagtcaaac gaccacgacc tacggctttc aagccgaact
    34621 caccgaaact taccttatta ccgcgagtag cgatgccagt attgcgaccc ttttgttgct
    34681 tacggtattt cagtctagtt ggctgcagca ttacgtctac ctgcctttct ttgtttactc
    34741 tcatgttcag gtttggaaga tttaatattg ccttccgtat aaacccaaac tttcagaccc
    34801 aatacaccat atgtggtgtg cgcttcgctg gttgcataat ctacatttgc acgtaaagta
    34861 tgcaatggca cgcgaccttc acgataccat tcgctacggg caatatccgc accattcaga
    34921 cggcctgaag tcataatctt aatgccttta gcaccagaac gcattgcatt ttgcattgct
    34981 cgtttcatag cacgacggaa ttgaacgcgc ttttccaact gctgggcaat accgtcagca
    35041 ataatttgag catccaactc aggacggcgg atctcttcaa tatttacatg aacaggtaca
    35101 cccatcaaga cttgcaagtc acgtttcaaa acctcgatat cctcaccttt tttaccgata
    35161 accacacccg gacgagcgga gtgaatggta atgcgtgcag attttgcagg gcgttcaata
    35221 accactcgac caaccgaagc attggccaat ttttgacgca aataattgcg aacatcgata
    35281 tcctgcttca aaacagtaga aaagtcggtg cttttagcaa accattttga agcccagtct
    35341 ttagttaccg ccaggcgaaa gcctgtaggg ttaatctttt gtcccatagc ttttccttag
    35401 ttacccactg tcacattgat atgacaagtt tgtttttcga tgcggttacc gcgacctttg
    35461 gcgcgagctt gaaaacgttt caagcttggg cctttgtcaa caaagatagt taccactttc
    35521 agttcatcaa tgtccgcacc gttattgtgc tcggcattag caatagctga ctccaatact
    35581 tttttaatca gctcggcacc ttttttagga ctgaaagcca aaatattcaa agcttgggca
    35641 acgtctttac cacgaatcaa atcagctacc aaacgagcct tttgagcaga gatacgggca
    35701 tttttatgtt gtgcatttac tctcatgatt caccttattt ctttttagcc tttttatcgg
    35761 ccaagtggcc tttaaaggta cgggtcaatg agaattcgcc taatttatga ccaaccatat
    35821 tgtcgctgat aaacacaggc acatgggtgc ggccgttgtg cacagcaatg gtcagaccga
    35881 taaaatcagg cagaatggta gaacgacgag accaggtttt aatcgggcgt ttgtcgttgc
    35941 ttgcgcgagc agcatctact tttttcagca aatgcaggtc tacatatggg ccttttttca
    36001 atgaacgagc catactaaat taacctttat ttgagtaacg gcgacgaaca atcatgttat
    36061 ccgtgcgttt gttattacga gtgcggtagc ctttagcagg agtaccccat gggctgaccg
    36121 gttcgcgggc ctcgcccgta cggccttcac caccaccatg cgggtgatcg acaggattca
    36181 tgacaacacc gcgtacggtc ggacgaatac cgcgccaacg gttggcaccg gctttaccga
    36241 tttttttcag gctttgctct tcgttaccaa cttcaccgat ggtcgcacgg caatttacgt
    36301 tgattttacg gacttcgcca gagcgcagac ggacttgagc gtacgcgcct tctttagcca
    36361 gcaataccgc agaagcaccg gcagaacgtg cgatttgagc acctttaccg ggtttcattt
    36421 cgatacagtg gatagtcgta ccaacgggaa tgttgcggat cggcagggtg ttgcctactt
    36481 tgatggcagc ttcagcgccg gaaactaata cagcaccggc ttgaataccg cgaggagcaa
    36541 tgatgtaacg acgctcgccg tctgcatagc acaacagtgc gatgaaggca gtacggttag
    36601 gatcgtattc gatacgctct actttagcag gaatgccgtc tttgttacgt ttaaagtcta
    36661 caacacggta atggtgttta tgaccgccgc ctttgtgacg ggtggtgata tgaccattat
    36721 tgttacggcc ggcagtagaa tttttctttt caagcaaggg tgcataaggt gcacctttgt
    36781 gcaaaccttc tgttaccacg cgaaccatgc cgcgacggcc tgcagaggtc ggcttcattt
    36841 taacgattgc cattttgttt attccttatc tgcagctgca gcagcggctt ccaaatccaa
    36901 ctcttgaccg gcagccaagc ttacataagc ttttttaaca tcgctgcggc ggcccaaagt
    36961 acgaccaaaa cgcttagttt tgcctttagt tgtaacggta gttacagaag caacttgaac
    37021 accgaacagc agctcgacag ccgctttgat ttcaggtttg gctgcatttg ccaaaacttt
    37081 aaacgtcatt tggttgcgtt tttcagccaa tacgttgctt ttttcagaaa cgataggtgc
    37141 caaaatcact tgagtcaaac gttgttgatt catacccatt gctcctctaa ttgtgcaact
    37201 gcatctttag tgatgattac ttttttgtaa cgcagcaagc tgtaaggatc aacttgttga
    37261 gcttccaaaa ccaacacgtt tggcaagttg cgtgaagcca agtaaacatt ctcgtcgagc
    37321 tgtttggtta caaacaacac ttgctccaga cccagatttt tcacttgttc ggcaaaaact
    37381 ttggttttag gagtttcggc agtcaacgcc tcaatcgcaa acaaacgctc gtcacgagtc
    37441 aattgggaca gaatagtcgc cataccggca cggtacattt tgcggtttac tttttgagtg
    37501 aagttttcgt cgggtttgtt cgggaacgcg cgaccacctt tacgccacag cggagaagaa
    37561 gtcataccgg aacgggcacg gccggtacct ttttgacgcc atggtttttt ggttgagtgt
    37621 tttacttcgg cacgggtttt ttgagcgcga ttgccggagc gggcgtttgc caaataggca
    37681 tttaccagct gatgaaccaa cgcttcattg tattcgcggg cgaacaaagc atcagaaaca
    37741 gacagactgc ctgaaacttg tcctttagcg tcaattactt tcaattccat tacgcaccta
    37801 ctttcacgct gggacgaact acaacatcgc tgttgaccgc acctggaaca gcacccttaa
    37861 ccaacagcag ttggcgttct gcatcaacac ggacaacttc caatttttga acagttgctt
    37921 tggtgttgcc gtattggccg gccatgcgtt taccggggaa cacgcgaccc gggtcttgcg
    37981 ccataccgat agagcctgga acgcggtgag agcgggagtt accgtgggaa gtacgttggg
    38041 caccgaagtt atgacgtttg atcgtaccgg agaaaccttt acctttagag gtaccggtta
    38101 catcgaccag ctggccgact tcaaacatag aaacggtgat ttcgtcaccg gctttcaatt
    38161 cagccagttt ttcttcagtc aaagcaaact caatcaaacc gcgaccggct tcaacacctg
    38221 cttttgcaaa gtgcccggct tcggctttat tgacacgatt ggcttttttc tgaccaaagg
    38281 taacttgaac ggcagtatag ccgtcagtat ctttggattt tacttgtgta acgcggttgg
    38341 cagacatatc caaaacggtt accggaacag aaacaccctg ttcgtcgaac acgcgggtca
    38401 taccaacttt gcgtccaacc agacctaaag tcatgattat tttcctttta aagtaaaggg
    38461 acaggctacg attggcctgt ctttcagaca aagttaaact tggcacaaat gtaccaagct
    38521 cggcattata tctgacaagt caaagaaata tcaacagaaa tatcagaaat atcttcaata
    38581 tgccgtctga agcgttttga attttcagac ggcatagggt cggactcttt taaagggtcg
    38641 gtatccggtc atcacagcgt atgcatcttt ctccgacaaa ccggaatatg gttgatgact
    38701 gcatccatta tttctttgag atggtagttt tcgtatgctg ggacctttgc aaaattcctt
    38761 tccctcccga cagccgaaac cccaacacag gttttcggct gttttcgccc caaatacctc
    38821 ctaattctac ccaaataccc ccttaatctt ccctggatac ccgataatca ggcatccggg
    38881 ctgcctttta ggcggcagcg ggcgcactta gcctgttggc cgctttcaac aggttcaaac
    38941 acatcgcttt caggtggctt tgcgcactca ctttgagcag cccgaaatag gctgcccgcg
    39001 catagcggaa tttacggtgc agcgtaccga agctttgttc gaccacataa cggggcttcg
    39061 acaaatatcg gttgcgtttg gtttgcgctt ccgacagcgg acggttgcgg caggctttgc
    39121 gcataatgcc gtccagcaac tgatgttctt tcagatgttg ccggttttcc gcactgtcat
    39181 agcctttatc ggcatagatg gtcgtacctt tgctcgatcg gctgccaatc aatcacttga
    39241 tccaacttca atagcgggaa acggtcgatg tgtttggcaa tcatggcttg tgcggtttgt
    39301 tgaaagaagg tgctcatgag aaatccccta aatgtcttgg tgggaattta ggagattttg
    39361 gggaattttg caaaggtctc ggcataacat aaaaaatccc caagcattga ttgcttgggg
    39421 attttcaata gtccgaatta ttggactttg atttctacat caacaccggc cgacaaatcc
    39481 agcttcatca gcgcatcggt agttttatcg gtccaatcca cgatgtccat caggcgcaag
    39541 tgggtgcgga tttccaattg ttcacgggaa gttttgttca cgtgcggaga acgcaaaatg
    39601 ttgaaacgct cgattttggt cggcaaagga atcgggcctt ttacaacggc accggtacgt
    39661 tttgcagttt caacgatttc ttgtgcagaa cggtcaatca gggcgtaatc ataagctttc
    39721 aggcggatac ggattttttg gtttgccatt tatcaatatc cttca
//
LOCUS       DAWFAD010000002        35837 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWFAD010000002 DAWFAD010000000
VERSION     DAWFAD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35837
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.002"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            81..992
                     /locus_tag="WGO49_000056"
     CDS             81..992
                     /locus_tag="WGO49_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0305227.1"
                     /translation="MLRLTALTLCTVLALGACSPQNSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDAKVPQNPERIAVYDLGMLDTLNKLGVKTGLSVDTNRLPYLEEYYKTTKPA
                     GTLFEPDYEALNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDEAIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKRQVVYLVPETYLAAGGA"
     gene            1064..1213
                     /locus_tag="WGO49_000057"
     CDS             1064..1213
                     /locus_tag="WGO49_000057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305228.1"
                     /translation="MPSETRMQTASCVSDGIARYGGFKQGFPLRRFGLPCLNLLRLNA
                     FPFCL"
     gene            1210..2178
                     /locus_tag="WGO49_000058"
     CDS             1210..2178
                     /locus_tag="WGO49_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0305229.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFIGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWLLLRKPAHAV"
     gene            2168..3142
                     /locus_tag="WGO49_000059"
     CDS             2168..3142
                     /locus_tag="WGO49_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ0305230.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCILFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELAVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCIGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLRHKK"
     gene            3187..3813
                     /locus_tag="WGO49_000060"
     CDS             3187..3813
                     /locus_tag="WGO49_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305231.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAAMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGIGMQGRFARRNLLSFGVGEINGTLTLRRKDNGKTVAVGLNAALQPFAPE
                     MRDIMPKAVSGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3896..4654
                     /locus_tag="WGO49_000061"
     CDS             3896..4654
                     /locus_tag="WGO49_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0305232.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLADTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDLSDRYLTELSGGQRQRAMIAMVFCQSTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPNDIFTAANIKTLFDMDVDVLDYEGKKLVIHHI"
     gene            4946..6163
                     /locus_tag="WGO49_000062"
     CDS             4946..6163
                     /locus_tag="WGO49_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HEZ0305233.1"
                     /translation="MKHFPSKVLTTAILATFCSGALAATNDDDVKKAATVAIAAAYNN
                     GQEINGFKAGETIYDIDEDGTITKKDATAADVEADDFKGLGLKKVVTNLTKTVNENKQ
                     NVDAKVKAAESEIEKLTTKLADTDAALADTDAALDATTNALNKLGENITTFAEETKTN
                     IVKIDEKLEAVADTVDKHAEAFNDIADSLDETNTKADEAVKTANEAKQTAEETKQNVD
                     AKVKAAETAAGKAEAAAGTANTAADKAEAVAAKVTDIKADIATNKDNIAKKANSADVY
                     TREESDSKFVRIDGLNATTEKLDTRLASAEKSITEHGTRLNGLDRTVSDLRKETRQGL
                     AEQAALSGLFQPYNVGRFNVTAAVGGYKSESAVAIGTGFRFTENFAAKAGVAVGTSSG
                     SSAAYHVGVNYEW"
     gene            complement(6229..6567)
                     /locus_tag="WGO49_000063"
     CDS             complement(6229..6567)
                     /locus_tag="WGO49_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ0305234.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERSDAAV"
     gene            6718..10680
                     /gene="purL"
                     /locus_tag="WGO49_000064"
     CDS             6718..10680
                     /gene="purL"
                     /locus_tag="WGO49_000064"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ0305235.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     NAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGDKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFELREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPIDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLHLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            10775..11533
                     /gene="gloB"
                     /locus_tag="WGO49_000065"
     CDS             10775..11533
                     /gene="gloB"
                     /locus_tag="WGO49_000065"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ0305236.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            11847..15066
                     /locus_tag="WGO49_000066"
                     /pseudo
     CDS             11847..15066
                     /locus_tag="WGO49_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254770.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            15142..16323
                     /locus_tag="WGO49_000067"
     CDS             15142..16323
                     /locus_tag="WGO49_000067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ0305237.1"
                     /translation="MKQTDPLTGILTRAQNLCAAQGYSVDICRQVAKAADTNDLTLFE
                     TELDTYIERVEMAESELDKARQGGDAQAVETARHAYLNALNRLSRQIHSLKTGVAGIR
                     MPNLAELISRSANTAVSEQAAYNTGRQQAGRRIDRHLTDPQQQNIWLETGTQQTDYHS
                     GTHRPYQQTTNYAHIGIQTGITDRLSVGTILTDERTNNRFDEGVSARNRSNGAHLFVK
                     GENGALFAAADLGYSNSRTRFTDYDGAAVRRHAWDAGINTGIKIDTGINLRPYAGIRI
                     NRSNGNRYVLDGAEINSPAQIQTTWHAGIRLDKTVELGQAKLTPAFSSDYYHTRQNSG
                     SALSVNDRTLLQQAAHGTLHTLQIDAGYKGWNAKLHAAYGKDSNTARHKQAGIKIGYN
                     W"
     gene            16555..18009
                     /gene="mgtE"
                     /locus_tag="WGO49_000068"
     CDS             16555..18009
                     /gene="mgtE"
                     /locus_tag="WGO49_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ0305238.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNIVWILVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(18095..19003)
                     /gene="hslO"
                     /locus_tag="WGO49_000069"
     CDS             complement(18095..19003)
                     /gene="hslO"
                     /locus_tag="WGO49_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ0305239.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENEWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGQGRLKMLVAEATSDRTVRATARWDEA
                     AEIGEDESLVDLLGEGGVFVLTLQPKDGEPWQGVVPLEGDGIAQMLMNYMKRSEQLDT
                     HIVLSASDEAAGGLLVQRLPEEVLDEEAWEHVSTLARTLTAGELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            19248..20003
                     /locus_tag="WGO49_000070"
     CDS             19248..20003
                     /locus_tag="WGO49_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0305240.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSISTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            20022..20240
                     /locus_tag="WGO49_000071"
     CDS             20022..20240
                     /locus_tag="WGO49_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305241.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            20385..20729
                     /locus_tag="WGO49_000072"
     CDS             20385..20729
                     /locus_tag="WGO49_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ0305242.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPDSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            20729..21421
                     /locus_tag="WGO49_000073"
     CDS             20729..21421
                     /locus_tag="WGO49_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ0305243.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVIVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            21488..22708
                     /gene="argJ"
                     /locus_tag="WGO49_000074"
     CDS             21488..22708
                     /gene="argJ"
                     /locus_tag="WGO49_000074"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ0305244.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLGFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVVD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQSAATVYTCD
                     LSHGYVSINADYRS"
     gene            22768..24177
                     /locus_tag="WGO49_000075"
     CDS             22768..24177
                     /locus_tag="WGO49_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0305245.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGVSIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLASGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLAAAKWLAT
                     VFSYWAGVPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            24366..25757
                     /gene="hrpA"
                     /locus_tag="WGO49_000076"
     CDS             24366..25757
                     /gene="hrpA"
                     /locus_tag="WGO49_000076"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ0305246.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     KRLAALPKPEFDNTLPVHEKLDEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     VAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIRETAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAV"
     gene            25858..26985
                     /locus_tag="WGO49_000077"
     CDS             25858..26985
                     /locus_tag="WGO49_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305247.1"
                     /translation="MKHLLIDFENVQPQNLDKLPTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            27163..28740
                     /gene="lpt3"
                     /locus_tag="WGO49_000078"
     CDS             27163..28740
                     /gene="lpt3"
                     /locus_tag="WGO49_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ0305248.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKATRLLIAVFFAFSIIANNVHYAVYQSWMTGINYWLMLKEITEVGG
                     AGASMLDKLWLPALWGMLEVMLFCSLAKFRRKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQSAPSKIGQGSVQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTQLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYLVPL
                     VLYSPDKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLNIRDGKAEYVYPQ"
     gene            28842..32105
                     /locus_tag="WGO49_000079"
     CDS             28842..32105
                     /locus_tag="WGO49_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEZ0305249.1"
                     /translation="MDARSNPANVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRLSEIKQLTSSENQGDQDLSAKRKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTKLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVPYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYNERLP
                     EMAWKDAQGSVWGNEDSVRIIESDKAERSSENERNEFRKNKRNGSRQNENHGNTVGWV
                     ENPTSAATAKTVGFDNPTYATQQPTPSPAREGRGEGKTVAAQTNFSATAANPLPQERE
                     QSAAASAVSNDLHPANPQQTAPSPVGEGWGEGKTVATQTNFSATTANPLPNPLPQEGE
                     QSAAASAVSNDPQPQKQPAPQKDRLKPLPLADIRTFQAWLKTAERENPRLLFLSRDDL
                     MQHAAAHITEEQFPKFWQTADGKFELSYRFEPHHPLDGVTLTLPLTVLNRISPAALEW
                     LVPGMLREKIQLLIKALPKQIRRICVPVPEFITQFLSQNPDRNAPILPQLAQAIAKTA
                     GDIRIFEQINQDEWAAFRLPEHCYFNLRIIDDGGQELAIGRDLIQIQQQLGQAAAVTF
                     RDNTQEFERDNVTTWDIGILPESIKFARGKQQLTGYLGLQKEKDGRIALRLFDTIEAA
                     EQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAF
                     IGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNSKLGKHPLTHLL
                     RQRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQM
                     WQEKTDSLIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLLKMWEDLN"
     gene            complement(32128..32550)
                     /locus_tag="WGO49_000080"
     CDS             complement(32128..32550)
                     /locus_tag="WGO49_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ0305250.1"
                     /translation="MTDIFTTSKRSFVMSKIHSKETKPEILVRKFLFSQGFRYRKNDK
                     RYAGKPDIVLPKYKTVVFIHGCFWYGHSCNKGHIPKSNTDFWLEKITKNCERDIKNET
                     ELEKIGFKVIVVWECELKNKAIGRERLNRLVREIKDAV"
     gene            complement(32554..34200)
                     /locus_tag="WGO49_000081"
     CDS             complement(32554..34200)
                     /locus_tag="WGO49_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HEZ0305251.1"
                     /translation="MSEEKLKMSFEPTVIEHLGVKMYSHTVPAIAELIANAYDACATE
                     VEVRLFDKPEHKIVIKDNGIGMSFDEINDFYLRIGRNRREEKQASPCGRIPTGKKGLG
                     KLALFGLGNKIEISTIQGNERVTFTLDYAEIKKSERIYQPEFQKESVEPNTENGTTIT
                     LTELTKKQGYPLDNYVEHLSRLFDFPAQDFKIKVSLNGSEPKIIDGNLKYDLVTPQFE
                     WEYQDLATNISSLSSKFEQYEYSGLIQGKFITTEKPLKNNMKGITLFANGRMVNMPEF
                     FTDSESSHFYSYLTGWLSIDFIDNDDEDLIATDRTSLDWKHPKTSKLRVFLGEIVRAI
                     GQDWRKRRKKEKDDEVKGESGVDIENWKNKLPEKEREPVEVILNRLEDSELTNKEQAE
                     VISALHSIIPEYPYYHWRHLHQDLHTACNDFYNEKKDYLSAAIEAVKVFENKVQKQTG
                     LHSIDGRELIEQAFGSKNSILLLTNNKTKAEQNLEDGLEQLACGTWTGFRNPVQHELR
                     ANLSPSIFNDKDALDLISLVSYLLRKVEQTKKRSKVVSSK"
     gene            complement(34214..35269)
                     /locus_tag="WGO49_000082"
     CDS             complement(34214..35269)
                     /locus_tag="WGO49_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0305252.1"
                     /translation="MKNSKLKAVDFFCGGGGMSYGMQSAGIQVLAGIDYEPSCKETYE
                     ANINAKFIQADVFELQPETLEKELGLKKNDDDLILIGCSPCQYWSVIQTDKRKSEKSK
                     SLLLEFQRFVEYFNPGYVVVENVPGILSRMKESRLDNFIKLLEEKGFTVHFGIHNTAD
                     YGIPQSRKRFTLIANRITKEKLEPVKYSGKRLTVRDVLGMENGFPKIIAGHQDETDFM
                     HSCAGLSDINLKRLALIPKNGGNRLAFAHIPELQLECFIGKDNSFKDTFGRLWWDKPA
                     PTITTKFFSISNGRFAHPEEDRALSLREGATLQSFPRNYVFKAGSRDKIARLIGNAVP
                     PMYAEKIGRAIVDNIEC"
     gene            complement(35669..>35837)
                     /locus_tag="WGO49_000083"
     CDS             complement(35669..>35837)
                     /locus_tag="WGO49_000083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305253.1"
                     /translation="VRESCTSNANPPLSELLKLINRQLTEKGLKIEKASAAVVDATII
                     QTAGSKQRQAR"
BASE COUNT         8989 a        10646 c         8521 g         7681 t
ORIGIN      
        1 ttatcattta tcctttctaa agccgttccg gtttgtccga ccggcggctt tgccccaata
       61 tccccatttt ggagacacct atgttgcgtt tgaccgcatt gaccctctgc accgtcctcg
      121 ccttgggcgc gtgttcgccg caaaattccg actctgcccc acaagccaaa gaacaggcag
      181 tttccgccgc acaatccgaa agcgcgtccg ttaccgtcaa aaccgcgcgc ggcgatgcaa
      241 aagtgccgca aaaccccgaa cgcatcgccg tttacgattt gggtatgctc gacactttaa
      301 acaaattggg cgtgaaaacc ggtttgtccg tcgacacaaa ccgcctgccg tatttagagg
      361 aatattacaa aacaacgaaa cctgccggaa ctttgttcga gccggattac gaagcactca
      421 acgcctacaa gccgcagctc atcatcatcg gcagccgcgc agccaaggcg tttgacaaat
      481 tgaacgaaat cgcgccgacc atcgaaatga ccgccgatac cgccaacctc aaagaaagtg
      541 ccaaagaacg tatcgacgcg ctggcgcaaa tcttcggcaa acaggcggaa gcggacaagc
      601 tgaaggcgga aatcgacgcg tcttttgaag ccgcgaaaac tgccgcgcaa ggcaaaggca
      661 aaggtttggt gattttggtc aacggcggca agatgtccgc cttcggcccg tcttcacgac
      721 tgggcggctg gctgcacaaa gacatcggcg ttcccgctgt tgacgaagcc atcaaagaag
      781 gcagccacgg tcagcctatc agctttgaat acctgaaaga gaaaaatccc gactggctgt
      841 ttgtccttga ccgcagcgcg gccatcggcg aagagggtca ggcggcgaaa gacgtgttga
      901 ataatccgct ggttgccgaa acaaccgcct ggaaaaaaag acaagtcgtt tacctcgttc
      961 ctgaaactta tttggcggcc ggcggcgcgt aagagctgct gaatgcaagc aaacaggttg
     1021 cggatgcttt taacgccgcg aaataatgaa acggcggcat tcgatgccgt ctgaaacacg
     1081 gatgcaaacc gcttcctgtg tttcagacgg cattgcccga tacggaggct tcaaacaagg
     1141 ctttccgctc cgacggttcg gactgccttg tttgaatctt ctacgcctta acgcttttcc
     1201 cttctgttta tgactgccaa acctttttcc ctcaacctga ccaacctcct gctgctggcg
     1261 gtattgtttg ccgtcagcct gtcggtgggc gttgccgatt tccgctggtc ggatgtgttt
     1321 tcgctatccg acagccagca ggtcatgttc atcagccgcc tgccgcgcac gtttgcgatt
     1381 gtgctgacgg gcgcgtctat ggcggtggcg ggcatgatta tgcagattct gatgcgtaac
     1441 cgttttgtcg agccttctat ggcgggtgcg ggtcagagtg cggctttggg tttgcttctg
     1501 atgtccctgc tgctgcctgc cgcgccgctg ccggtcaaaa tgtcggttgc cgccgttgcc
     1561 gcgctaatcg ggatgctggt gtttatgctg ctgatccgcc gcctgccgcc gacggcgcaa
     1621 ctgatggtgc cgctggtggg gattattttc ggcggcgtgg ttgaggcggt ggcgacgttt
     1681 atcgcgtatg aaaacgaaat gctgcaaatg ctcggcgtgt ggcagcaggg cgatttttcg
     1741 agcgtgctgc tggggcggta cgagctgctt tggattacgg gcggtttggc ggtgtttgcc
     1801 tatctgattg ccgaccggct gacgattttg gggctgggcg agacggtcag cgtgaatttg
     1861 ggtttgaacc ggacggcggt gttgtggtcg gggctgatta ttgtagcact gattacatcg
     1921 ctggtcattg taacggtcgg caatattccg tttatcgggc tggtcgtgcc gaacatcatc
     1981 agccgcctga taggcgacag gctgcgccaa agcctgcctg cggtggcctt gttgggcgcg
     2041 tctttggttt tattgtgcga cattatcgga cgcgtgattg tgtttccgtt tgaaattccg
     2101 gtctctacgg tttttggtgt gttgggtacg gctttgtttt tgtggctttt gttaaggaaa
     2161 cctgctcatg ccgtctgaaa aaaatatcgg ttttatggca ggaagcagcc gtccgttgtg
     2221 ggttgccttt gcgctgttgc tggtttcctg catcctgttt atgacgctca acgtcaaagg
     2281 cgattgggac tttgtactgc acctgcgtct gaccaaactt gccgcgctgc tgatggtcgc
     2341 ctatgcggtc ggcgtgtcca cgcaactctt ccaaacgctg accaacaatc cgattctgac
     2401 cccttcaatt ttgggtttcg attcgctgta tgtgtttttg cagaccttgc tggtgtttac
     2461 gttcggcggc gtgggctatg cttccctgcc gttgacgggc aaattcggct ttgaacttgc
     2521 cgttatgatg ggcggctcgc tgctgctgtt ttacacgctc atccgtcagg gcggacgcga
     2581 tttgccgcgt atgattttaa tcggcgtgat tttcgggatt ttgttccgca gcctgtcgtc
     2641 gctgctttcg cgcatgatcg accccgaaga atttacggcg gcgcaggcga atatgtttgc
     2701 cggattcaat accgtccaca gcgagctttt gggcataggc gcgctgattc tgctcgtcag
     2761 cgcggcggtc gtttggcgcg aacgctaccg cttggacgta caccttttgg ggcgcgacca
     2821 agccgtcaat ttgggcatca gctacacgcg caacaccttg tggatactgc tttggattgc
     2881 cgcattggtg gcgacggcga ccgccgtggt cggcccggta agctttttcg ggcttctcgc
     2941 cgcctcgctt gccaaccact tttccccgtc cgtgcgccat tccgtccgcc tgccgatgac
     3001 ggtttgtatc ggcggtatcc tcttggtcgg cggacagacc gtattcgaac acttcttggg
     3061 catgaaggcg gtattaagcg tggtggtcga atttgcgggc ggactcgttt tcctctatct
     3121 cgttttaaga cacaaaaaat gacggatgcc atctgaacgg cagcccgccc cgaaaggaca
     3181 aaccatatga cgcaagaacg tttacccgaa tttttcgacc gcgccccgac gctgaccgta
     3241 caagacccgc ttgccgcatt cctcggcgcg gccgaaaacg gcatcctcac ttaccgctac
     3301 gccgatgccg tgcgcctgtg cggacattcc tgcccgaccg tcgcgggcgc gtacctgatg
     3361 gttatcaaag gtctgaaagc cctttacgga gaagagctgc ccgaacgcgg cggcatcgaa
     3421 gccgccatgc agggagcgcg cgacgaaggc acggtcggcg taaccgcgtc cgtcgtccaa
     3481 cttctcaccg gcgcagcccc cgaaaccggc ttcggaggca tcggaatgca gggacgcttc
     3541 gcccgccgca acctcttatc ctttggtgta ggcgaaatca acggcacact gaccctgcgc
     3601 cgcaaagaca acggcaaaac cgtcgccgtc ggcctcaacg ccgccctgca acccttcgca
     3661 cccgaaatgc gcgacatcat gcccaaagcc gtcagcggca gcgcaagcgc agaagaactc
     3721 gaacgcttcg gacaactctg gcaggcacgc gttaaagcat ttttaaccga atcggcggac
     3781 gacccgcagt tcgtcatcgt ccgcgaagtg tgagcgttca gacggcattc cgaatttcaa
     3841 atgccgtctg aaccccgcca aacaacaaca aacctacgcc cgacaagcat ccgccatgat
     3901 taccatccgc aacgtcagct accgcatcgg cacacgcccc atcctcgaca acgtcagcct
     3961 cgacatcccc gaaggcggca ttaccgccct cgtcggcccc aacggtgcgg gcaaatccac
     4021 cctgttttcc tttatggcgc ggctgcgacc gcttgaaagc ggcagcatcg cctaccgagg
     4081 caaaaatctt gccgataccc ccaccgccga actcgccaaa accctgtcca tcctcaccca
     4141 agaaaacagc atcatgagcc gcatcaccgt gcgcgacctg ctgatgttcg gccgttaccc
     4201 ctaccatcaa ggcagaccga ctgccgaatg ccgccgtatc gttaacggtg caatcgaaga
     4261 attccacctg caagacctct ccgaccgcta cctgaccgag ctttccggcg gccaacgcca
     4321 acgcgccatg attgcgatgg tgttctgcca aagcaccgac tacgtccttt tggacgaacc
     4381 gctgaacaac ctcgatatgt atcacgcccg ctcgctcatg caaatcctgc gccggctgac
     4441 cgacgaacac aagcgcacca ccgtcgtcgt attgcacgac atcaaccagg cagcagccta
     4501 cgccgaccac gtcgtcgcca tgaaaaacgg ccaagtcgcc atgcagggca aacccaacga
     4561 tattttcacc gccgcaaaca tcaaaaccct attcgatatg gacgtcgacg tcctcgatta
     4621 cgaaggcaaa aaattggtta tccaccatat ctaaatccga caaaaaggcc gtctgaacat
     4681 tcagacggca acccatatcc tgacaaaatt aagacacgac accggcagaa ttgacatcag
     4741 cataatatgc acatattaac agatattaat gccgaactac ctaactgcaa gaattaaata
     4801 aataaataaa taaataaatt gcgacaatgt attgtatata tgcctccttt catatatact
     4861 ttaatatgta aacaaacttg gtggggataa aatacttaca aaagatttcc gccccatttt
     4921 ttatccactc acaaaggtaa tgagcatgaa acactttcca tccaaagtac tgaccacagc
     4981 catccttgcc actttctgta gcggcgcact ggcagccaca aacgacgacg atgttaaaaa
     5041 agctgccact gtggccattg ctgctgccta caacaatggc caagaaatca acggtttcaa
     5101 agctggagag accatctacg acattgatga agacggcaca attaccaaaa aagacgcaac
     5161 tgcagccgat gttgaagccg acgactttaa aggtctgggt ctgaaaaaag tcgtgactaa
     5221 cctgaccaaa accgtcaatg aaaacaaaca aaacgtcgat gccaaagtaa aagctgcaga
     5281 atctgaaata gaaaagttaa caaccaagtt agcagacact gatgccgctt tagcagatac
     5341 tgatgccgct ctggatgcaa ccaccaacgc cttgaataaa ttgggagaaa atataacgac
     5401 atttgctgaa gagactaaga caaatatcgt aaaaattgat gaaaaattag aagccgtggc
     5461 tgataccgtc gacaagcatg ccgaagcatt caacgatatc gccgattcat tggatgaaac
     5521 caacactaag gcagacgaag ccgtcaaaac cgccaatgaa gccaaacaga cggccgaaga
     5581 aaccaaacaa aacgtcgatg ccaaagtaaa agctgcagaa actgcagcag gcaaagccga
     5641 agctgccgct ggcacagcta atactgcagc cgacaaggcc gaagctgtcg ctgcaaaagt
     5701 taccgacatc aaagctgata tcgctacgaa caaagataat attgctaaaa aagcaaacag
     5761 tgccgacgtg tacaccagag aagagtctga cagcaaattt gtcagaattg atggtctgaa
     5821 cgctactacc gaaaaattgg acacacgctt ggcttctgcc gaaaaatcca ttaccgaaca
     5881 cggtacgcgc ctgaacggtt tggatagaac agtgtcagac ctgcgtaaag aaacccgcca
     5941 aggccttgca gaacaagccg cgctctccgg tctgttccaa ccttacaacg tgggtcggtt
     6001 caatgtaacg gctgcagtcg gcggctacaa atccgaatcg gcagtcgcca tcggtaccgg
     6061 cttccgcttt accgaaaact ttgccgccaa agcaggcgtg gcagtcggca cttcgtccgg
     6121 ttcttccgca gcctaccatg tcggcgtcaa ttacgagtgg taagcagcat ctcccgataa
     6181 agaaaccgca gccctgcaag gctgcggttt ttattttcta tccggccgtc aaaccgccgc
     6241 gtccgaacgt tcgcccgtgc ggatacggat tgcctcctcg accggcagca caaaaatctt
     6301 gccgtcgccg atttttcccg aacgcgccac ctcgacaatc acgtcaatcg cgcgttccac
     6361 agcatcatcc gccaacacca gctcgatttt gaccttgggc aggaaatcga cagcgtattc
     6421 cgcgccccgg taaatctccg tatgcccctt ctgcctgccg aaccctttga cctcgctgac
     6481 ggtcataccc gtaatgccga tttccgtcaa cgcctcgcgc acgtcgtcga gtttgaacgg
     6541 tttgacaatc gcctcgattt ttttcataaa atttcctttg aacaaacaat acaaacacat
     6601 ccgaaaaacg ggaacctccc gtcagattgt caacatttta agccaaaata cccaagcaat
     6661 acagccccgt tgcgcgtata atgacagatt ttccaaccgc atttgagagc cgaatccatg
     6721 tctgtcgttt tgcccttgcg cggcgttacc gccctttccg atttccgtgt cgaaaaactc
     6781 ttgcaaaaag ccgccgcact cggactgccc gaagtcaaat taagcagcga attttggtat
     6841 ttcgccggca gcgagaaagc attggatgcc gcgactgtcg aaaaactgca agccttgttg
     6901 gcggcgcaaa gcgttgaaca aacgccaaaa gcgcgcgagg gcttgcattt gtttttggta
     6961 acgccccgtt tgggtacgat ttcgccgtgg gcttccaagg cgaccaatat cgcggaaaac
     7021 tgcggtttgg caggcatcga gcgcatcgag cgcggcatgg cggtgtggct ggaaggtgcg
     7081 cttaccgatg aacagaaaca gcaatgggcg gcgttgctgc acgaccgcat gaccgaatcc
     7141 gtgctgcccg attttcagac ggcatccaaa ttattccacc atctcgaatc cgaaaccttt
     7201 tccaccgttg atgttttagg cggcggtaaa gaggctttgg tcaaagccaa taccgaaatg
     7261 ggtttggcac tctccgccga cgaaatcgat tatctggttg aaaactatca ggctttgcaa
     7321 cgcaatccgt ccgatgtgga gctgatgatg ttcgcgcagg caaacagcga acactgccgc
     7381 cacaaaatct tcaacgccga cttcatcctc aacggcgaaa aacaaccgaa atcccttttc
     7441 ggcatgatac gcgacacgca caacgcgcat cccgaaggca cggtcgtcgc ttataaagac
     7501 aattcgtccg tgatcgaagg cgcgaaaatc gagcgtttct atccgaatgc ggcggaaaac
     7561 caaggctacc gtttccacga ggaagacacg catatcatca tgaaagtgga aacgcacaac
     7621 cacccgaccg ccatcgcgcc gtttgcaggc gcggcaacgg gcgcgggcgg cgaaatccgc
     7681 gacgaaggcg caacgggcaa aggttcgcgt ccgaaagcag gcttgaccgg ctttaccgtg
     7741 tccaacctca acatccccgg cctcaaacag ccgtgggaac aagattacgg caagccggaa
     7801 catatttcct cgccgctgga catcatgatt gaaggcccga tcggcggcgc ggcgttcaac
     7861 aacgaattcg gccgccccaa cctcttgggc tacttccgca cttttgaaga aaaatttgac
     7921 ggtcaggttc gcggctacca caaaccgatt atgattgccg gcggcttggg cagcattcag
     7981 gcgcagcaga cgcataaaga cgaaatcccc gaaggcgcgt tgctgatcca actgggcggc
     8041 ccgggcatgc ttatcggctt gggcggcggc gcggcttctt cgatggatac cggcacgaac
     8101 gacgcttctt tagacttcaa ctccgtacaa cgcggcaacc ccgaaatcga acgccgcgcg
     8161 caggaagtca tcgaccgctg ctggcagctc ggcgacaaaa acccgattat ctccatccac
     8221 gacgtgggcg caggaggttt gtccaacgcc ttccccgaac tggtcaacga tgccggacgc
     8281 ggcgcggtat tcgagctgcg cgaagtgccg ttggaagaac acggtttaac gcctttgcaa
     8341 atctggtgca acgaatcgca agagcgttat gtgttgtcga ttttggaaaa agatttggat
     8401 accttccgcg ccatctgcga acgcgaacgc tgcccgtttg ccgtggtcgg tacagcgacc
     8461 gacgacggtc atttgaaagt gcgcgacgat ttgttcgcca acaaccccat cgatttgccg
     8521 ttgaacgtct tgctcggcaa accgcccaaa accacgcgta ccgacaaaac agttacgcca
     8581 tctaaaaaac cgtttcacgc cgacgatatc gacatcaccg aagccgccta ccgcgttttg
     8641 cacctgcctg ccgtagccgc caaaaacttc ctgattacca tcggcgaccg cagcgtcggc
     8701 ggcatgaccc accgcgacca aatggtcggc aaataccaaa cccccgtagc cgactgcgcc
     8761 gttaccatga tgggcttcaa tacctatcgc ggcgaagcaa tgtctatggg cgaaaaaccg
     8821 accgtcgccc tgtttgacgc gcctgcttcg ggcagaatgt gcgtcggcga agccatcacc
     8881 aacatcgcgg cggtcaacat cggcgacatc ggcaacatca agctctccgc caactggatg
     8941 gcggcgtgcg gcaacgaagg cgaagacgaa aaactctacc gcaccgtcga agcggtttcc
     9001 aaagcctgtc aggcattgga tttgagcatt cccgtgggca aagacagcct gtcgatgaaa
     9061 accgtatggc aggatggcga agagaaaaaa tccgtcgttt cgccgttgag cctgattatc
     9121 tccgcgttcg cgccggttca agacgtacgc aaaaccgtta cgcccgaatt gaaaaacgtc
     9181 gaggacagcg tattgctgtt tgtcgatttg ggcttcggca aagcgcgtat gggcggctct
     9241 gcattcggtc aggtatacaa caacatgagc ggcgacgcgc ccgatttgga cgacacaggc
     9301 cgtctgaaag cgttttacaa cgtgattcaa cagcttgtcg ccgaagacaa actcttggca
     9361 taccacgacc gcagcgacgg cggcttgttt gccgttttgg tagaaatggc gtttgcaggg
     9421 cggtgcggct tggatataga tttaaattta ttgcttgcac aaacatttat taccaaccat
     9481 accgctctgt ctcaatcatt gcggactgaa gaggtaaaag cgttggctga atggcaagaa
     9541 accattgccc gcacattatt taatgaagag ttgggtgctg ttatccaagt tagaaaacaa
     9601 gatgttgccg atattatcaa tttattctat caacaacagc tgcatcataa tgtctttgaa
     9661 atcggtacgt taactgatga gaacacgtta atcatccgcg acgggcaaac gcaccttatt
     9721 tctgacaacc taatcaaact gcaacaaacc tggcaagaaa ccagccatca aatccaacgc
     9781 ctgcgcgaca accctgcctg cgccgacagc gagtttgccc tgattggcga caacgaccgc
     9841 agcgcattgt ttgccaacct gaaattcgac gtgaacgaag acatcgccgc gccgtttatc
     9901 aacagcggcg cgaaacccaa aatcgccatc ctgcgcgaac agggcgtgaa cgggcaaatc
     9961 gaaatggccg ccgccttcac ccgcgccgga ttcgatgctt acgacgtgca tatgtccgac
    10021 ctgatggcag gccgcttccg ccttgccgac ttcaaaatgc tggcggcgtg cggcggcttc
    10081 agctacggcg acgtactcgg cgcgggcgaa ggctgggcga aatccatcct gttccaccct
    10141 gccttgcgcg accaatttgc cgccttcttt gccgatccgg acacgctgac attgggcgtg
    10201 tgcaacggct gccagatggt cagcaacctc gccgagatta tcccgggtgc agaggcatgg
    10261 cctaaattca aacgcaacct gagcgaacag tttgaagcac gcctgagcat ggttcacgtt
    10321 ccgaaatcag cgtcgctgat tctgaacgaa atgcaaggct ccagcctgcc tgtcgtggtc
    10381 agccacggcg aaggccgcgc cgacttcgcg cttcacggcg gcaatatttc cgccgatttg
    10441 ggcatcgcgc tgcaatatgt ggacggtcaa aaccaagtga cccaaaccta cccgctcaac
    10501 cccaacggct cgccgcaagg catcgccggc gttaccaacg ccgacggccg cgtgaccatc
    10561 atgatgccgc atccggaacg cgtgtaccgt gccgcacaaa tgagctggaa accagaagac
    10621 tggacggaat tgtccggctg gtaccgcctc tttgccggcg cacgtaaagc cttgggctaa
    10681 ccgccctact caaaccaatg ccgtctgaag aatatttcag acggcgttcc ggcataccat
    10741 cctttaaacg gtatccgtcc accgaggaac actcatgaaa atcacccccg tcaaagccct
    10801 aaccgacaac tacatctgga tgatacagca cggcaaccat gccgtctgcg tcgacccttc
    10861 cgaaccctcg cccgtcttgg aattcctcgt ccgcaaccgc ctcatgcttg cccaaacatg
    10921 ggtaactcac ccccaccccg accacgaggg cggtgcggcg gcactctggc gcggctacat
    10981 ggaatcgccc gtttacggcg aatccgacat cgaagcagca acccacaccg taaccgccgg
    11041 cacccaattc accttcggcg acggacaggt taccgtttgg gcaacacccg gccacacaga
    11101 ccgccacacc agctaccttc tcgaaacttc agacggcata cacgttttct gcggcgacac
    11161 cctgttttcc gccggctgcg gacgcgtgtt taccggcaca atcgaacagc tttacgacag
    11221 cttccaacgc ttcaaccgcc tgcctgaaaa caccctgttc tacccggcac acgaatacac
    11281 cgccgccaac ctggattttg ccgcccatat cgagccggac aacgccgaca ttcagacggc
    11341 actgaaagcg gcggcgcata cgcctaccct gcccgttacc ctcgcgcacg aacgccgcgt
    11401 caatccgttt ttgcgcgtcg acctgccgca cgtcagagac cgcgccgagg cattgagcgg
    11461 gaaaacgtta aacagcagcc tcgatacctt tgtcgcgctg cgtgaactta aaaaccaata
    11521 ccggacgaaa taaaacaacg ggaaaacgca gccattccta ggatttttat taaaatctta
    11581 aataaaatca tacaatcatc gccaatagac gaaaggacac cgttgcctta taatcaaaca
    11641 aaaacaaaat atataatata gtggattgaa tttaaatcag gacaaggcga cgaagccgca
    11701 gatagtacgg caaggcgagg caacgctgta ctggtttttg ttaatccact atattgttaa
    11761 tccactatat aaatccagca caaaacggga tcggtgattc ttgtccgcaa gaatcgttga
    11821 ttttctctat tacacggata atcatcatgc gcttcacaca caccacccca ttttgttccg
    11881 tattgtccac cctcggtctt tttgccgttt cccctgctta ctcatccatt gtccgcaacg
    11941 atgtcgatta ccaatatttt cgcgactttg ccgaaaataa aggcgcgttc gccgtaggtg
    12001 caagcaatat ttccatccaa gacaagcaag gcaaaatatt aggcagggtt ctcaacggca
    12061 tccccatgcc cgacttccga gtcagcaacc ggcaaaccgc catcgccacc ctgattcacc
    12121 cccaatacgt caacagtgtc aaacacaacg tcggctacgg ttccatacaa ttcggcaacg
    12181 acacccaaaa tccagaagaa caagcctata cctaccgcct cgtatcacgc aacccgcacc
    12241 cggactacga ctaccacctt ccccgcctca acaaactggt taccgaaatc tcacctgccg
    12301 cactcagcag cgtacccttg cttggaaacg gccagccaaa ggccaatgcc tacctcgata
    12361 ccgaccgctt cccctacttt gtacgactcg gctcaggcac gcaacaagtc cgcaaagcag
    12421 acggcacgcg tacacgaacc gccccggcat accaatacct gaccggcggc acgccgctga
    12481 aagtattggg gttccaaaac cacggcttac tcgtcggcgg cagcctgacc gaccaacccc
    12541 ttaacaccta cgcaatcgcc ggagacagcg gttcccccct gtttgccttc gacaagcatg
    12601 aaaaccgctg ggtgcttgcg ggcgtactca gcacctacgc cggcttcgat aatttcttca
    12661 acaaatacat cgtcacgcaa cccgaattca tccgttccac catccgccaa tacgaaaccc
    12721 ggctggatgt cgggctgacc accaacgaac tcatatggcg cgacaacggt aatggcgaca
    12781 gcaccctgca agggctcaac gaacgcatca ccctgcccat tgcaaaccct tcgcttgccc
    12841 cacaaaacga cagcaggcac atgccgtctg aagatgccgg caaaacgctc atcctatcca
    12901 gcaggttcga caacaaaaca ctgatgctgg cagacaatat caaccaaggc gcaggcgcat
    12961 tgcagttcga cagcaacttc accgtcgtcg gtaaaaacca cacatggcaa ggtgcaggcg
    13021 ttatcgtagc cgacggcaaa cgcgtcttct ggcaagtcag caaccccaaa ggcgaccggc
    13081 tctccaaact gggcgcaggc acgcttatcg ccaacggaca aggcatcaac cagggcgaca
    13141 tcagcatcgg ggaaggcact gtcgtactcg cccaaaaagc tgcttcagac ggcagcaaac
    13201 aagcattcaa ccaagtcggc atcaccagcg gcaggggtac ggccgtcctc gccgacagcc
    13261 agcaaatcaa atccgaaaac ctctatttcg gcttcagggg cggacggctc gacctcaacg
    13321 gcaacaacct tgcctttacc catatccgcc atgcggacgg cggcgcgcag atcgtcaacc
    13381 acaaccctga ccaagccgcg acactgacgc tgaccggcaa ccccgtcctc agtcctgagc
    13441 atgtcgagtg ggtgcaatgg ggcaaccgtc cgcaaggcaa cgcggcggtt tacgaataca
    13501 tcaacccgca ccgcaaccgt cggaccgact acttcatact caaacccggc ggcaacccgc
    13561 gcgaattttt cccgttaaat atggaaaact caacaagctg gcaatttatc ggcaacaaca
    13621 ggcaacaggc cgccaaacaa gtcgcccaag ccgaaaatgc ccgccccgac ctaattacct
    13681 tcggcggata cttgggtgag aacgcgcaaa cgggcaaagc cgcaccgagt tacagcaaaa
    13741 ccaatatcga atgggtgaat ggtccaaaca ccccacacaa gccctcgccc tatacgaata
    13801 cgccaacccg cacaggggca ggcgtaccga cgattaccgc ctcaaagaag gcggcaaccc
    13861 cggcgcctac ttcccatgga atgcctacag cacccaagac tgggaatttc tcggacatga
    13921 ccggcgaagc agccatagaa aaaaaccgcc atatcgcaaa tgccgccgta tacggccggc
    13981 ccgaataccg ttacaacggc gcactcaacc tgcactatcg tcccaaacgc accgacagca
    14041 cgctgttgct caacggcggc atgaacctta acggggaagt cttgattgag ggcggcaata
    14101 tgattgtgtc aggcaggccc gtaccccatg cctacgacca ccaggccaaa cgcgaacccg
    14161 ttcttgaaaa cgaatggacc gacggcagct tcaaggctgc acggttcacc ctacgaaacc
    14221 atgcccgact gacagcaggg cgcaataccg cgcatctgga cggcgacata accgcataca
    14281 atctgtccgg catcgacctc ggctttatcc aaggcaaaac accggaatgc taccgctcct
    14341 accatagcgg cagcacccac tgcacaccca acgccgtttt aaaagccgaa aactatcgtg
    14401 cactacctgt aacgcaagta cgcggcgaca ttacccttaa cgaccgttca gagctccgcc
    14461 tgggcaaagc acacctgtac ggcagcatcc gtgccggcaa agacaccgca gtccgcatgg
    14521 aagcagacag caactggaca ctttcccagt ccagccacac cggcgcactg acgcttgacg
    14581 gcgcacaaat taccctgaac cccgatttcg ccaataatac acacaacaac cgcttcaaca
    14641 cactgaccgt caacggcaca cttgacgggt tcggcacatt ccgattcctg accggcatcg
    14701 tccgaaaaca aaatgccccc ccccctcaaa ctggaagggg acagccgcgg cgcattccaa
    14761 atccacgtca aaaacaccgg acaagaacct caaacaaccg aatcgcttgc acttgtgagc
    14821 ctcaatccga aacacagcca ccaagcccga ttcaccctcc aaaacggcta tgccgatttg
    14881 ggtgcctacc gctacatcct ccgcaaaaac aacaacggat acagcctgta caacccgctc
    14941 aaagaggccg aacttcaaat tgaagccacg cgtgcggaac atgagcgcaa ccaacaggca
    15001 tacaaccaat tacaggcaac cgacatcagc agacaggttc aacatgactc tgacgcgacc
    15061 aggtaggcac tacaggcctg gcagaacagt caaaccgaac ttgcccgcat cgacagccaa
    15121 gtccaatatc tgtccgccca attgaaacag acagacccgc tgaccggcat tctgacgcgt
    15181 gcccaaaacc tgtgtgccgc acaaggatac agtgtcgata tctgccgtca ggttgccaaa
    15241 gccgccgaca cgaacgacct gacactcttc gaaaccgaac tggatacgta tatagaacgt
    15301 gtagaaatgg ccgaatccga acttgacaaa gcacggcaag gcggcgatgc gcaagccgtc
    15361 gaaacagccc ggcacgccta cctgaacgca ctcaaccgtc tgtcccgaca aatccacagt
    15421 ttgaaaaccg gcgttgccgg catccgtatg ccgaacctgg ccgaactgat cagccggtcg
    15481 gccaacaccg ccgtttccga acaggccgcc tacaataccg gccggcaaca ggcgggacgc
    15541 cgcatcgacc gccaccttac cgatccgcag cagcaaaaca tctggctgga aaccggtacg
    15601 caacaaaccg actaccatag cggcacacac cgtccctacc aacaaactac caactatgca
    15661 catatcggca tccaaaccgg catcaccgac cgtctcagtg tcggtacgat tttaaccgat
    15721 gagcgcacaa acaaccgttt tgatgaaggc gtatccgccc gaaaccgcag caacggcgca
    15781 catctgttcg tcaaagggga aaacggcgca ctctttgccg cggcagattt aggctacagc
    15841 aacagccgta cccgatttac cgattatgac ggggctgccg tccgccgcca cgcatgggat
    15901 gcaggcatca acaccggcat caaaatcgat accggcatca acctcagacc ctatgccggc
    15961 atccgtataa accgcagcaa cggcaaccgg tacgtactcg acggcgcaga gataaacagc
    16021 ccggcgcaaa tccaaaccac atggcatgcc ggcatccgtc tcgataaaac cgtcgaactg
    16081 ggtcaagcca agctgacccc cgctttcagc agcgattact accatacccg ccaaaacagc
    16141 ggttccgccc tcagcgtcaa cgaccgtacc ttactgcagc aagccgccca cggcacactg
    16201 cataccctgc aaatcgacgc cggatacaaa ggctggaacg ccaaacttca tgccgcttac
    16261 ggcaaagaca gcaacaccgc ccgccacaaa caggcaggaa tcaaaatagg ctacaactgg
    16321 taacaagccg ataaaaatgc cgtctggaac ccgcgtttca gacggcattt gcgttaaaaa
    16381 tagtaaaccg ttccaaaagg gagtagaata gtgccgtttc caaccctgcg cctgtaccgt
    16441 caggctttta ttatggacct tcccagttcg tttttactga acgccccttc cgattccaaa
    16501 cgacaataac catcccgccg gaatgcctcc ccgcacacga cgggcggagc atttatgagc
    16561 atcgaacaca ctcctccgac acacgacggc gaaaccggtc aaaaccatgc cgaacgccct
    16621 tccgctgatt tcgaccgtgt ccactccctc tgcgaaatcc tcgaacctgc ctttgaacaa
    16681 atcgaaaacg gcacaccgct cgaagacgcg ccgctgcgcg acaagctgac cgagctgacc
    16741 gtcctcttga gcgagctgca ccctgccgac gtggcggcag tgttggaatc gctgccgccg
    16801 cgcgaacgca atatcgtctg gattctggtc aaaccggaag acgacggcga agtcttgctg
    16861 gaagtgtccg atgcggtgcg cgaaacgctg atcgagtcga tggacaaaga cgaattgttg
    16921 gcggcggtcg atgatttgga cgcggacgaa ttggcggagc tggcagacga tttgccgcac
    16981 caagtggttt atgaagcctt gcagacgcgc gacgaggaag agcgcgcaca agtcaaagcg
    17041 gcaatgtcct acgaagacaa ccaagtcggt gcgattatgg acttcgagtt ggtcagcatc
    17101 cgcgccgatg tcgcctgtga agtggtgctg cgctatctgc gccgcttcga cagcctgccc
    17161 gaccataccg acaagatttt tgtggtcgat gaaaacgacg tactgcaagg cgtgctgccc
    17221 atccgcaaac ttttggtcgc cgatcccgaa gatttggtgg aaaacgtgat ggcgaaagat
    17281 gtcgtgcgtt tccgcgccga agacgacgtg gaagaagcag cgcaggcatt cgaacgctat
    17341 gacttggtta ccgcgcccgt cgtcgatgaa aacaaaaagc tcatcggcag ggtgaccgtt
    17401 gacgaaatgg tggacgtgat ccgcgaagaa tccgaagcgg atatgttgaa catggcaggt
    17461 ttgcaggaag aggaagacct gttcgccccc gtcttggatt ccgtcaaaaa ccgctggatg
    17521 tggctcgccg tcaacctctg caccgccttc ctcgccagcc gcgtcatcgg cgcgtttgaa
    17581 ggcagcatcg aaaaaatcgt cgcactcgcc gcgctgatgc ccatcgtcgc cggcatcggc
    17641 ggcaactcgg gcaaccagac gattaccatg attgtccgcg cgatggcgat ggggcaactg
    17701 acaggcatgc aggcaggcag gctgttgaaa aaagaagtcg gtgtcgcctt ggtcaacggc
    17761 atcatttggg gaacggtcat gggcgcagta tcttggctgc tttacggcag cctcggcatc
    17821 gggctggtta tgattgccgc gatgacgctc aacctcctgc tggcggcaac cgtcggcgta
    17881 ttaattcccg tggtaatgga aaagttcgga cgcgatcccg cactgggcag ctcggtgctg
    17941 attaccgccg ttaccgactc cggcggcttc ctgattttct tggggctcgc caccctattc
    18001 ctgctttaaa tgccgtctga acccgcgcaa aaatgccgtc tgaagcggaa gctgcttcag
    18061 acggcatttg actatttatc cttgttgcac aagattattg gacggtatgc cggggcagcc
    18121 ctttggcaac gccgaccaca tcctccccga acagcgcgtt gacatcggtt tcgtcaaaca
    18181 catatttgct gtggcagaaa tcgcaatcga cttcgatgct gccttgttcc accaccacgc
    18241 cgccgacttc ttccccgccc agcatcaaca gcatatcgct gactttgccg cgcgaacagg
    18301 tgcatgaaaa ttcaaacgtt tccggctcga acacgcgcgg cggcgtttcg tggaacaagc
    18361 ggtacaaaac atgttgcgca tccaattctg ccaactcgcc cgccgtcagc gtgcgcgcca
    18421 gcgtactgac gtgttcccat gcctcttcat ccaacacctc ttcaggcaga cgctgcacca
    18481 gcagaccgcc cgccgcttca tcgcttgcag acaggacgat gtgcgtatca agctgttcgg
    18541 aacgtttcat atagttcatc aacatttgcg cgataccgtc gccttccaaa ggcactacgc
    18601 cctgccaggg ttcgccgtct ttgggctgca gcgtcagcac gaatacgccg ccctcgccca
    18661 aaagatcgac caaactttca tcttcaccga tttctgcagc ctcgtcccac cgcgcggtcg
    18721 cacggacggt acggtcggaa gtcgcttccg caaccagcat tttcagccgc ccctgcccct
    18781 gaacctgcac aatcagcgtg ccttcgtttt tgaggttgcc cgacagcaga acaccggcag
    18841 ccaacagctc gcccaaggcg cggcggatgg cggcgggata gtttttctgt tttacaatgt
    18901 gctgccactc gttttccaga cggacgtgca gcccgcgcac gggcatatcg tcgaagataa
    18961 agcgggtacg cacatcggcg cggttgatgg cggtttgatt catgattttc tctgactgat
    19021 tgttcggatg gcggctatat ggttgcggtc ggcgcgaaaa caagacggac ggcggatgcg
    19081 cttcccaaat tatcaataaa ttatataaaa atcaacatat taactcaatc taacaagccg
    19141 ttttttgcca aacagccgtt tttttatata caatcaacaa gatattttcg actgatacag
    19201 cataacatcg cacggcggca cgatgcctcc tgcgcggaaa caccgatatg gattcttttt
    19261 tcaaaccggc agtttgggcg gttttgtggc tgatgtttgc cgtccgcccc gcccttgccg
    19321 acgagttgac caacctgctc agcagccgcg agcagattct cagacagttt gccgaagacg
    19381 aacagcccgt tttacccatc aaccgagccc ccgcccggcg ggcgggcaat gccgacgaac
    19441 tcatcggcag cgcgatgggg cttaacgaac agcccgtttt acccgtcaac cgagtccccg
    19501 cccggcgggc gggcaatgcc gacgaactca tcggcaacgc gatggggctt aacgaacagc
    19561 ccgttttacc cgtcaaccga gcccccgccc ggcgggcggg caatgccgac gaactcatcg
    19621 gcaacgcgat gggacttttg ggtattgcct accgctacgg cggcacatcg atttctaccg
    19681 gttttgactg cagcggcttc atgcagcaca tcttcaaacg cgccatgggc atcaacctgc
    19741 cgcgcacgtc ggcagaacag gcgcggatgg gtacgccggt tgcccgaagc gaattgcagc
    19801 ccggggatat ggtgtttttc cgcacgctcg gcggcagccg catttcccat gtcggacttt
    19861 atatcggcaa caaccgcttc atccacgcgc cgcgcacggg gaaaaatatc gaaatcacca
    19921 gcctgagcca caaatattgg agcggcaaat acgcgttcgc ccgccgggtc aagaaaaacg
    19981 acccgtcccg ctttctgaac tgatttccca aggaatacgc aatgagtatg cccgaaatgc
    20041 ccaaatggta cgacgatgac ggacagatcg tgtcctgtac cgaaaaggtc aaagtgatgt
    20101 ccgaaaatat ggccgagctg tatcagacgg cacaagacgc gtttgaagac gcgctgctga
    20161 tgggttgcgg cgaacgtcag ttgcgcgatt acctgctcgc gctgattgaa ggtttggaaa
    20221 atccctaccg caaagtctga acacgccccg gttgctgcgg cacggtttat ccgtgccgtt
    20281 tttgcgtttg tgcgcggctt cggcttttca gacggcatat ttgacgttat gattaaacag
    20341 ttaacaagat ttatcacaac gccgtcaaaa gacagacaca caacatgaac atcatccgcg
    20401 cgctcctcat catcctcggc tgcctcgccg ccggcgaaac cgccgttttc ctagcaggca
    20461 tcaaactgcc cgacagcatc gtcggcatgg gcgtgctgtt tgcgcttttg caggcaggtt
    20521 gggtcaaaac gtcttggctg caacagctta ccgacgcgct gatggcgaat ctgacgctgt
    20581 ttctcgtgcc gccctgcgtg gcggtcatca gctatttgga tttgattgcc gacgattggt
    20641 tttcgatact ggtttccgcc tccgccagca ctttgtgcgt actgctggtt acaggcaagg
    20701 ttcaccgctg gatacggagc attatctgat gaacgaaatc ctcaggcagc ccagcgttct
    20761 gcttttcctc acgcttgccg tgtacgcgct tgcgattatc gtgcgcacgc gcacgggcaa
    20821 tatcttctgc aaccccgtac tcgtcagcac tatcgtgctg attgcctacc tgaaaatcct
    20881 cgacatcgat tatgcggtgt accacaacgc cgcgcaattc attgattttt ggctgaaacc
    20941 cgccgtcatc gtgcttgccg tgccgctcta ccaaaaccgc cgtaaaatct tcaaccagtg
    21001 gctgcccgtc atcgtttcac agcttgcggg cagcgttacg ggcattgtta cggggatgta
    21061 ttttgccaaa tggctgggcg cggaacgcga agtcgtcctc tcgctcgcgt ccaaatctgt
    21121 taccaacccc atcgccattg aaatcacccg ctccatcggc ggcattcccg ccattaccgc
    21181 cgccaccgtc atcattgccg gtctggtcgg acagattgcc ggttacaaaa tgctgaagaa
    21241 cacagtcgtc atgccctcgt ccgtgggtat gtcgctcggc acggcttcgc acgcgatggg
    21301 gattgccgcc tcgctcgaac gcagccgccg catggcggca tacgcgggac tggggctgac
    21361 gttcaacggc gtactgaccg cgctgattgc gccgctgctc atccccgttt tgggattttg
    21421 aacccgtttc agacggcatt tcagcccatg ctgtctgaac gccgacacac tcgcaaggag
    21481 aaccgttatg gctgtcaacc tgactgaaaa aaccgccgaa caactgcccg acatcgacgg
    21541 cattgccctc tacaccgccc aagcaggcgt gaagaagccc gggcataccg acctgacact
    21601 gattgccgta gccgccggca gcaccgtcgg tgcagtcttc acgaccaacc gtttctgtgc
    21661 cgcgcccgtc cacatcgcca aatcgcacct tttcgacgaa gacggcgtgc gcgccctcat
    21721 catcaacacg ggcaacgcca acgcgggtac gggcgcacag ggcagaatcg atgctttggc
    21781 agtgtgtgcc gccgccgccc ggcaaatcgg ctgcaaaccg aaccaggtgc tgcccttctc
    21841 caccggcgtg attctcgaac cgctgcccgc agacaaaatc atcgccgcgc tgcccaaaat
    21901 gcagcctgcc ttctggaacg aagcggcacg cgccatcatg accaccgaca ccgtgcccaa
    21961 agccgcctcg cgcgaaggca aggtcggcga caaacacacc gtccgcgcca cgggcatcgc
    22021 caaaggctcg ggcatgattc atcccaatat ggcgaccatg ctcggtttca tcgccaccga
    22081 tgccaaagtt tcccaacccg tcctccaact gatgacgcag gaaatcgccg acgaaacctt
    22141 caacaccatc accgttgacg gcgacaccag caccaacgac agcttcgtca tcatcgccac
    22201 cggcaaaaac agccaaagcg aaatcgacaa catcgccgac ccgcgttacg cccaactcaa
    22261 agaattgttg tgcagcctcg cgctcgaact cgcccaagcc atcgtccgcg acggcgaagg
    22321 tgcgaccaag ttcatcaccg tccgcgtcga aaacgccaaa acccgcgacg aagcccgcca
    22381 agccgcctac gccgtggcac gttcgccgct ggtcaaaacc gcctttttcg cctccgaccc
    22441 caacctcggc aggctgctcg ccgccatcgg ttatgccggc gttgtcgacc tcgatactga
    22501 cctcgtggaa atgtatctcg acgatatttt ggttgccgaa cacggtggac gcgccgcaag
    22561 ctacaccgaa gcacaagggc aggcggtgat gtcgaaggcc gaaatcaccg tccgcatcaa
    22621 gctgcatcgc ggacaaagtg ccgccaccgt ctatacctgc gacctgtcgc acggatacgt
    22681 ttccatcaac gccgactacc gttcctgacc cgacacggct tcagacggca tacataaaat
    22741 gccgtctgaa ccgccggaca acatgccatg acctccacat tcccccgccg cctcgcccgc
    22801 aaaatccgcc aaacccgccg cctgtcgcgc aaaagcatcg cctttctgtt ccttttggca
    22861 ggttcggcac tcgtcgccct gaccgcgctg ttttttgccc atcttgccga ttttgcgctg
    22921 gaactgaacg ccaaactggt tcaacaatac ccgtggttcg cgtgggtcgc gcttcctttg
    22981 ggtttgccgc ttattgcgtg gctcacacgc aaattcgccc ccttcaccgc cggcagcggc
    23041 atcccgcagg tcatcgcctc actgtcgctg ccctacggcg cacagaaaac gcggctgatc
    23101 cgcctcgggc agacgctgct gaagattccg ctgacctttt tgggtatgct gttcggcgtg
    23161 tccatcggac gcgaaggtcc gtccgtgcag gtcggcgcgg cagtgatggg cgcgtggggc
    23221 gcgtggtgca agaaacacgg cttggcattc aaagggatgc aggaaaacga tttgatggcg
    23281 gcgggcgcgg cgggcggttt ggcagccgcg ttcaacgcgc cgctggcggg cgtgattttc
    23341 gccattgagg aactcgggcg cggcatcatg ttgcgctggg agaggcaaat tcttttgggc
    23401 gtgctcgcct ccggtttcat acaggtcgcc attcagggca acaacccgta tttttccggc
    23461 ttcaacggcg gcgtattgga acatatcttt ctgtgggtcg cactgtccgg cctggtttgc
    23521 ggcgcggcgg gcggcctgtt cggacgtttg ctctatcgcg gtgcggcggc gtttgcaccg
    23581 cgcaagatac gcggcttcat ccgcaaccgt ccgctgctgc tggcggcact gatggggctg
    23641 ctgctcgccc tgctcggcac gttctaccaa ggcaaaacct acggcaccgg ctaccacgaa
    23701 gccgcccaag ccctgcacgg catctacgaa gcccccttcg gactcgccgc cgccaaatgg
    23761 ctcgccaccg tattcagcta ttgggcaggc gttccgggcg gcattttcac tccctcgctg
    23821 accataggcg cggttttggg cgagcatatc gccgccatcg ccgacatatc gcagggtgca
    23881 aacatcatcg tcctcatctg catggcggca tttctggcgg gcgcgacaca atccccgatt
    23941 acttccgccg tcgtcgtcat ggaaatgacg ggcggacaaa gcctgctgtt ttggatgcta
    24001 attgcctgca ttttcgcctc gcaggtttcg cgccagtttt cgccgcgtcc gttctaccac
    24061 gcatcgggaa tgcgcttccg ccagcgcgtg cttcaagaaa ccgccgccca aaccggcaat
    24121 gcgcccgcaa gaccgcaaac agcaaacagc aaaacgggaa tgccgtctga aaattaaaac
    24181 gcccccgatc aaacgccggc agccgccttg atttgaatac cgttccgccg ccgcttgaaa
    24241 tttcagcaac aatgccgtct gaacgacaga atgcggtttt cagacggcat ttccccatcc
    24301 cgatattgcc taaacaaaac cgaagcgttt gctataattg cactttttac cgcacacgca
    24361 cgcccatgtt tcccgatttc tcccaaaccc tctccaaaga ccgccacttc ctgcaatccg
    24421 ccttcaaaaa tcccaacaaa tacggcggct tgtccaaagt cgaagaaaaa taccgaaaat
    24481 cgcacgaaat ctttttgaaa cgtttggcag ccttgcccaa acccgaattc gacaacaccc
    24541 tgcccgttca cgaaaaactt gacgaaatca aaaaaaccgt tgccgaacat caggtaacga
    24601 ttatttgcgg cgaaaccggt tcgggcaaaa ccacgcagtt gcccaagatt tgtttggaac
    24661 tcgggcgcgg ggtagcgggt ttgatcgggc atacccagcc gcgccgtttg gcggcgcgtt
    24721 cggtggcaga gcggattgcc gaagagctga aatccgaaat cggcagcgcg gtcggctata
    24781 aagtacgctt caccgaccac acctcgcgcg atgcctgcgt caagctgatg accgacggca
    24841 tcctgctggc ggaaacgcag accgaccgtt atctcgccgc ctacgacacg attatcatcg
    24901 acgaagcgca cgagcgcagc ctgaacatcg acttcctttt gggctatttg aaacaactcc
    24961 tgccgcgccg ccccgatttg aaagtcatca tcacctcggc aacgatagac gcagaacgct
    25021 tctcccgaca cttcaacggc gcgcccgttt tagaagtgag cggacggacg tatcccgtcg
    25081 aaatcctcta ccgaccgctg accggcaaag acgaagacga cgcagaagtg gagttgaccg
    25141 acgcgattgt cgatgcggcg gacgaattag cgcgacacgg cgaaggcgat attttggtat
    25201 tcctgccggg cgagcgcgaa atccgcgaaa ctgccgaagc cttacgcaaa tctacactgc
    25261 gccgcaacga cgaaatcctt cccctgttcg cacgcctgtc gcacgccgag cagcacaaaa
    25321 tcttccaccc ttcaggcgcg aaacgccgca tcgtattggc aaccaacgtc gccgaaacct
    25381 cgcttaccgt gccgggcatc aaatacgtca tcgacaccgg cctcgcgcgc gtcaaacgct
    25441 attccgcacg ggcaaaggtg gagcagcttc atgtcgaaaa aatctctcaa gccgccgccc
    25501 gccaacgctc cggccgctgc ggacgcgtct ccgcaggcgt gtgtatcaga ctgttttcag
    25561 aagaagattt caacagccgc cccgaattta ccgacccaga aatcgtccgc agcaacctcg
    25621 ccgccgtcat cctgcgcatg gcatcgctga acctgggcga tgtggcggca ttcccatttt
    25681 tagaaatgcc cgattcgcgg tatatcaatg acggttttca ggtattgctg gaattggggg
    25741 cggtagaggc cgtctgaaaa caagtaggat atacacttct acctgacaaa gaccaacaaa
    25801 cgataaaact acggcacatc tagatgccca acctacaaca ttgcacacgg taaaaacatg
    25861 aaacacttac tcatcgattt tgaaaacgtc cagccgcaaa acttagacaa attaccaacc
    25921 gaaaataccc atatttggct atttataggt gtattacaca aaatgttacc tattagtctg
    25981 gtgcaatccc tactacgttt cggcgaacgt gtccatcttg tccagttaca aaaaacgggg
    26041 aaaaacgcat tggattttta cctgtcctat tacctcggac aaattaccgc cacagacccc
    26101 aatgcccaaa tcggcatact ctcgcgtgat ggaggatacg atgttctggt cgaacatatt
    26161 ttgaaaaacc accaggcgaa gggtatcgtg cgcctagcca atatagatga agtacaacat
    26221 cagaaaatta ctaccgaacc gccgtcagca ttgctggaaa acactcctca gcctgaaacc
    26281 accctcaaac cacagcaacc attaacttcc tatttccaag cagccctaac tgcactgcgc
    26341 cgccccgacg ctttccgccc ctgccgcctg cataacctgc gacaaaatct gcgtaagcat
    26401 attttgagtg atttgtttaa agaaaaaacc gatgaagaat gcgaaataac cactgctaac
    26461 gttatcaata aactcaaagc acaaaacttc atcagcattg atgaacagga aaccgtttcc
    26521 taccatctca gtgataatga tttgttacaa agaatccaac gccatatttt aagccaacgt
    26581 cccaaaacct acgctgattt tcaagccgtc gtgcaaaacc gagcagatgc acttcactta
    26641 acagtcggta ccaacgacat tcaatccttt gcgcgacatt tgcgcgacca aaacctgatc
    26701 cgccaaaaca atgggaaaat tgaatatgca ccgtttactg aacctaaacc acagccaacg
    26761 cccaagcagc ctaaaaaaac cgcatgggaa cctgatgaaa ttatttggaa aaaagtgatt
    26821 gccgcgttat cgttaaagaa ccgtcctaat aaaaccaaaa ctttacgcaa tacaatccag
    26881 gcactcacaa aatccaatgc acaagaaact gacaaactgc tacaacattt acaagatacc
    26941 caagtcctac gtattgacgg tactaaaatt gtgtatatca agtaggatgg gcatttatgc
    27001 ctgacctaca aaaccaaaaa caacctacca cccttaatca actccacaaa cccttttcag
    27061 acaacctcgt tttttgaaaa acaatctgta aacagatagc tgctgaagaa taccgttgcc
    27121 gagccccaaa acccgtcctg caacttttat tgtgaacttc ccattatgaa aaaatccctt
    27181 ttcgttctct ttctgtattc gtccctactt actgccagcg aaattgctta tcgctttgta
    27241 ttcggaattg aaaccttacc ggctgcaaaa atggcagaaa cgtttgcgct gacatttgtg
    27301 attgctgcgc tgtatctgtt tgcgcgttat aaggcgacgc gtttgttgat tgcggtgttt
    27361 ttcgcgttca gcattattgc caacaatgtg cattacgcgg tttatcaaag ttggatgacg
    27421 ggcatcaatt attggctgat gctgaaagag attaccgaag ttggcggcgc gggggcgtcg
    27481 atgttggata agttgtggct gcctgcgttg tggggcatgt tggaagtcat gttgttttgc
    27541 agccttgcca agttccgccg taagacgcat ttttctgccg atatactgtt tgccttccta
    27601 atgctgatga ttttcgtgcg ttcgttcgac acgaaacaag agcacggtat ttcgcccaaa
    27661 ccgacataca gccgtatcaa agccaattat ttcagcttcg gttattttgt cggacgcgta
    27721 ttgccgtatc agttgtttga tttaagcaaa atccctgtgt tcaaacagtc tgcgccaagc
    27781 aaaatcgggc agggcagtgt tcaaaatatc gtcctgatta tgggcgaaag cgaaagcgcg
    27841 gcgcatttga aattgtttgg ctacgggcgc gaaacttcgc cgtttttgac ccagctttcg
    27901 caagccgatt ttaagccgat tgtgaaacaa agttattccg caggctttat gacggcagta
    27961 tccctgccca gtttctttaa cgtcataccg catgccaacg gcttggaaca aatcagcggc
    28021 ggcgatacca atatgttccg cctcgccaaa gagcagggct atgagacgta tttttacagt
    28081 gctcaggctg aaaaccaaat ggcaattttg aacttaatcg gtaagaaatg gatagaccat
    28141 ctgattcagc cgacgcagct tggctacggc aacggcgaca atatgcccga tgagaagctg
    28201 ctgccgctgt tcgacaaaat caatttgcag cagggcaggc attttatcgt gttgcaccaa
    28261 cgcggttcgc acgccccata cggcgcattg ttgcagcctc aagataaagt attcggcgaa
    28321 gccgatattg tggataagta cgacaacacc atccacaaaa ccgaccaaat gattcaaacc
    28381 gtattcgagc agctgcaaaa gcagcctgac ggcaactggc tgtttgccta tacctccgat
    28441 catggccagt atgttcgcca agatatctac aatcaaggca cggtgcagcc cgacagctat
    28501 ctcgtgccgc tggtgttgta cagcccggat aaggccgtgc aacaggctgc caaccaggct
    28561 tttgcgcctt gcgagattgc cttccatcag cagctttcaa cgttcctgat tcacacgttg
    28621 ggctacgata tgccggtttc aggttgtcgc gaaggctcgg taacgggcaa cctgattacg
    28681 ggtgatgcag gcagcttgaa cattcgcgac ggcaaggcgg aatatgttta tccgcaatga
    28741 gtggcgtaaa aaccaataaa gacaaattta gatgatgtcg gggaagatgc ccgaccgaca
    28801 agactatgca aaatatgaaa aaccaagtac gcggatcagg catggatgca cgatccaatc
    28861 cggccaatgt ttcagacggc ctgcaaaaca gttcgggtca tatcggtacc aacacgcgtt
    28921 accgcctgac caaactcggc gaacaaatgg cgcgcctgcc catcgacccg aaaatcgcgc
    28981 gcattttgtt ggcggcgaag aagcacgact gcatggcgga aatattggtg atcgcgtccg
    29041 cgctgtcgat tcaagacccg cgcgagcggc cgctggaagc gcgcgatgcc gccgccaagg
    29101 cgcacgagcg ttttaccgac aagcaatctg actttcttgc ctatctgaac atttgggaca
    29161 gcttccagcg cgagcgcgac aagggcttgt ccaacaagca gttggtgcag tggtgccgtc
    29221 aatatttcct gtcgcacctg cggatgcgcg agtggcgcga gctgcaccac cagcttgccc
    29281 aaaccgcgat tgaaatgggt ttgaccacca aggaagcggc tttcagacga ctttccgaaa
    29341 tcaagcagct tacgtcgtct gaaaaccaag gcgatcaaga cttatccgcc aaacgcaaac
    29401 aaaaacaact ggataagaaa caacaccgcg cccaaatccg cgccgccaaa gaagcgggct
    29461 acgaacaaat ccaccgcgcc ctgctcaccg gccttatcgc caacgtcggc atgaaatcgc
    29521 ccgacggcaa cgactatacc ggcgcgcgcg gcagccgttt ccaccttttc cccgcctccg
    29581 ccctgttcaa agccaaaccc aaatgggtga tggcggcaga actcgttgaa acgacaaaac
    29641 tttatgcgcg cgacgtcgcc gttatccagc ccgaatggat tgagcaggaa gccccgcacc
    29701 tcgtccgcta ccactatttc gagccgcact gggaacaaaa acgcggcgaa gtcgtcgcca
    29761 gcgaacgcgt gacgctttac ggcctgaccg tattgccgcg ccgccccgtg ccttacggca
    29821 aagttgcgcc cgaagaagca cgtgaaatct ttatccgcag cgcgttggtg gcgcaggaat
    29881 gcgatttgaa agcggatttt tttgtccaca ataaaaagct gattaaagaa attaccgaac
    29941 tcgaacacaa atcgcgcaag caagacgtat tggtcgacga cgaagccctg tttgcgtttt
    30001 ataacgaacg cctgcccgaa atggcttgga aagacgcgca aggcagcgtt tggggaaatg
    30061 aagattccgt acggattatt gaatctgaca aagccgagag gtcgtctgaa aatgagcgca
    30121 acgagtttcg taaaaacaag cgtaatgggt ctcgccaaaa tgaaaatcac ggcaacaccg
    30181 taggttgggt tgaaaaccca acatcagccg caactgcaaa aactgttggg tttgacaacc
    30241 caacctacgc tactcagcaa cccaccccct cccccgcgag ggagggtcgg ggagagggca
    30301 aaacagttgc cgcacaaacc aacttttccg caaccgcagc aaaccctctc ccgcaggaga
    30361 gggagcagag tgccgcagct tcagcggttt caaacgacct gcatcctgca aatccccaac
    30421 aaaccgcccc ctcccccgtg ggggagggct ggggagaggg caaaacagtt gccacccaaa
    30481 ccaacttctc cgcaaccaca gcaaaccctc tccctaaccc tctcccacag gagggggaac
    30541 agagtgccgc agcttcagcg gtttcaaacg accctcaacc ccaaaagcag ccagcacccc
    30601 aaaaagaccg tctgaaaccc ctccccctcg ccgacatccg caccttccaa gcctggctca
    30661 aaaccgccga acgcgagaac ccgcgcctgc tgttcctcag ccgcgacgat ttgatgcaac
    30721 acgccgccgc acacattacc gaagaacaat tccccaaatt ctggcaaacc gcagacggca
    30781 agttcgaact cagctaccgc ttcgagccgc accacccgct cgacggcgtt accctcaccc
    30841 tgccgcttac cgtgctcaac cgcatcagcc ccgccgccct cgaatggctc gtgcccggta
    30901 tgttgcgcga gaaaatccag ctgctgatta aagcactgcc caagcaaatc cgccgcatct
    30961 gcgtgcccgt gcccgaattc atcacccaat ttttaagcca aaaccccgac cgcaacgccc
    31021 ccatcctgcc ccaactcgcc caagccatcg ccaaaaccgc aggcgacatc cgcatattcg
    31081 agcaaatcaa ccaagacgaa tgggccgcgt tcaggctgcc cgaacactgc tatttcaacc
    31141 tccgcatcat cgacgacggc ggacaagaat tagccatagg gcgcgattta atccaaatcc
    31201 aacaacaact cggccaagcc gccgccgtta ccttccgcga caacacccaa gaattcgagc
    31261 gcgacaacgt gaccacatgg gacatcggca tcctgcccga atccatcaaa ttcgcacgcg
    31321 gcaaacaaca gcttaccggc tatctcggcc tgcaaaaaga aaaagacggc cgcatcgccc
    31381 tgcgcctgtt cgacacaata gaagccgccg aacaggcaca ccgtcaaggc gtgatagaac
    31441 tcatgaaact acaactaaaa gaacaagtta aagacctgaa caaaggcatc caaggcttca
    31501 cccaagccgc catgctgctc aaacacatca acgccgacac cctgcgcgac gacctcaccc
    31561 aagccgtctg cgaccgcgcc tttatcggcg aagacgagct gccgcgtaac gaaaaagcct
    31621 ttaaagagca aatcaaacgc gcccgcagcc gcctgcctgc cgtcaaagaa gccctcagcc
    31681 gctacctgca ggaaaccgca gccgcctacg ccgaactcaa cagcaaactc ggcaaacacc
    31741 cattgaccca cctcctacgc caacgcctgc aaaccctgct cgccgccggc ttcgccaccc
    31801 gcaccccgtg ggcacaatgg ccgcgcctcc ccatctacct caaagccatg accctgcgcc
    31861 tcgaaaaata cagcagcaac cccgcccgcg acgcagcccg cgaagccgat atccaagagc
    31921 tggaacaaat gtggcaggaa aaaacagaca gcctgattaa acaaggtctc cccatttcag
    31981 acggcctcgc cgcgtttaaa tggatgattg aagaattgag ggtgtcgctg ttcgcgcagg
    32041 aattgaagac accgtatccg gtgtcggtga agcggttgtt gaagatgtgg gaggatctta
    32101 attaaaggaa gatgccgtct gattttttca gacggcatct tttatttccc tgaccaaccg
    32161 atttaatcgc tccctaccaa tagctttatt ttttaattcg cactcccaaa cgacaatcac
    32221 tttgaaaccg atcttttcca gctcggtttc atttttaata tcacgttcgc aattttttgt
    32281 gattttttcc aaccaaaaat ccgtattgct tttcgggata tgtcctttat tacaagaatg
    32341 cccgtaccaa aaacagccgt gtataaacac gactgtttta tatttcggca aaacgatatc
    32401 cggtttgcct gcatagcgtt tatcgttttt ccgataacga aaaccttggg agaaaagaaa
    32461 tttccttacc aatatttccg gtttggtttc tttgctatgg atttttgaca tcacgaaaga
    32521 acgtttggat gtagtgaaaa tatcggtcat aaattacttg gaagatacca ccttcgatcg
    32581 tttttttgtc tgttccactt ttcttaacaa atacgaaacc aagcttatta aatccaaagc
    32641 atccttatca ttaaaaatac taggagataa atttgctctt aactcatgtt gtacaggatt
    32701 tctaaatcca gtccatgttc cgcatgcaag ctgttcaaga ccatcttcta aattttgttc
    32761 cgccttcgtt ttgttattcg ttaatagaag tatagaattt ttactcccga aagcttgttc
    32821 aatcaattct cttccgtcta tgctatgaag acctgtttgc ttctgaacct tattttcgaa
    32881 aactttaact gcctcgattg cagcacttaa ataatccttc ttttcattgt aaaaatcatt
    32941 acaggcagta tgcaaatcct gatgcaaatg ccgccaatga tagtaaggat attctggaat
    33001 aatactatgt aaggcagaga ttacttccgc ttgctcttta tttgtaagct ccgaatcttc
    33061 caaacgattc aaaataactt ctactggttc cctttccttt tctggcaatt tgtttttcca
    33121 attttcgata tcaaccccgc tttcaccttt cacttcgtca tctttttctt tttttcttct
    33181 ttttctccaa tcttgcccga tggctcgaac gatttcccca aggaaaaccc gtaactttga
    33241 agttttagga tgcttccaat ccaatgatgt cctatctgta gcaattaaat cttcatcatc
    33301 attatctata aaatcaatac ttaaccatcc tgtcagatag gaatagaaat ggctggattc
    33361 gctatcagtg aaaaactcgg gcatatttac cattctgccg ttggcaaaca aggtaatacc
    33421 tttcatatta ttctttaaag gtttttccgt tgtaatgaac ttaccttgta ttaatccgct
    33481 gtattcatac tgttcgaatt ttgaagataa cgatgaaata ttggttgcta gatcctggta
    33541 ttcccattcg aattgtgggg taacaagatc atattttaga tttccatcaa tgattttggg
    33601 ttccgagccg ttcaagctta ctttgatttt aaaatcctga gccggaaaat caaacaagcg
    33661 ggaaagatgc tccacataat tatctaacgg gtatccttgt tttttcgtca gttcggttaa
    33721 agttatggtt gttccgtttt cggtattggg ttcaacagat tctttctgaa actccggttg
    33781 ataaatacgc tcactttttt taatctctgc ataatccaaa gtaaaagtaa cccgttcgtt
    33841 tccttggata gtagaaattt caattttgtt gccaagcccg aataatgcca atttaccaag
    33901 gcctttttta cccgttggaa ttcttccgca cggggaggct tgtttttctt cccttctgtt
    33961 ccgaccgatt ctcaaataaa aatcattgat ttcatcgaag ctcattccta taccattatc
    34021 tttaataacg attttatgct ccggtttatc gaataaccta acttccactt ccgtagcaca
    34081 tgcatcgtag gcattcgcta tcaactcggc aatcgcagga acagtgtgcg aatacatctt
    34141 tacacccaaa tgttcgatta cggttggctc gaaactcatt ttcaattttt cttcagacat
    34201 gttttctcct tttctaacat tcgatattat caacaattgc cctgcctatt ttttccgcat
    34261 acatcggagg aacggcatta ccaatcaaac gggctatctt gtccctgctg cccgctttaa
    34321 agacatagtt acgagggaac gattgcaatg ttgcaccttc gcgcagtgat aacgccctat
    34381 cttcttcagg atgggcaaac ctcccgttgg aaatactaaa gaatttggtc gttatggtag
    34441 gtgcgggctt atcccaccat agcctaccaa acgtgtcttt aaaactattg tcttttccga
    34501 taaaacattc cagctgtaat tcgggaatat gcgcaaaagc caaacggttt cctccgtttt
    34561 taggtatcaa agccaatcgt ttcaagttga tatcagataa tcccgcacag ctatgcataa
    34621 aatccgtttc gtcttggtgt cctgcaataa ttttgggaaa gccattttcc attcccaaaa
    34681 catcgcgtac cgtaagccgt ttgcccgaat acttgactgg ttccagcttt tctttggtta
    34741 ttctgtttgc aattaacgta aatcttttac ggctttgggg aattccgtaa tcagccgtat
    34801 tgtgaatacc gaaatggacg gtaaatcctt tttcttccaa taactttatg aaattatcaa
    34861 gcctgctttc tttcattctg ctcagaattc ccggtacgtt ctctacaaca acatatccgg
    34921 gattgaaata ttctacaaac cgctgaaact ccaaaagcaa actcttggat ttttccgatt
    34981 ttcttttgtc tgtttgaata acactccaat attgacaagg actacaacct ataagaatca
    35041 aatcatcatc attctttttt aacccgagtt ctttttccaa ggtttcaggc tgcaattcaa
    35101 aaacatcagc ctgtataaat ttggcattta tattggcttc atatgtttct ttgcagctgg
    35161 gttcgtaatc tatcccggct aaaacctgaa taccggcgga ttgcattcca tagctcattc
    35221 cacctccgcc acagaaaaaa tcaaccgcct ttaacttact gtttttcatt tctatttaaa
    35281 taattagact agtgagaact aactttatat aacatataat ttattgctta tcaaaaataa
    35341 tttgagattt tattaaaatt catgcatcta aataagcaga acttaatttc ctccaaacga
    35401 gcggatgtct tcaaatgcac acttcacaga aaggtcgtct gaaacccatt ttcagacgac
    35461 ctttttccac ttcccaaaac aagaattccc accccctccc caaacgtcgc ccgaaccagt
    35521 aaactcccca tatttcaaca acttcttgga agccaaccat gccccgtatc caccctcctc
    35581 acctgttccc gccccatatc gccgaacgcg gccttttgta ttttcagcag ggcaaggttc
    35641 gcgatgtccg aaaaacttcc gccgggcgtt atcgggcctg gcgctgtttg ctgccggcgg
    35701 tctgaataat ggtggcgtca acgacggcag cggatgcttt ctctattttt aagccttttt
    35761 cggtcagttg gcggttaatc agtttgagca attcggacag gggcggattc gcatttgaag
    35821 tgcaactttc cctaaca
//
LOCUS       DAWFAD010000003        33739 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWFAD010000003 DAWFAD010000000
VERSION     DAWFAD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33739
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.003"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..428)
                     /locus_tag="WGO49_000084"
     CDS             complement(<1..428)
                     /locus_tag="WGO49_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305254.1"
                     /translation="MDQAKRTAYVKIINALFDGAVKVQPSQITETVVDMVDKMFDEIA
                     TCHERIRPMAVLADGIAGSIVKLAPNEIPLIFGNSSFGSYLKSKTRDEVFKVLKQSKV
                     NNAGKVLEAFYESWLKYTAKDRRLKVCIVTAQSRWRSRFE"
     gene            complement(499..4764)
                     /locus_tag="WGO49_000085"
     CDS             complement(499..4764)
                     /locus_tag="WGO49_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0305255.1"
                     /translation="MGGKSGGGQRTPYEAPNSLSSAQSLRIIDAVSEGVVSGFANGDD
                     APFKSVYFDDTPVQNSDGSYNFNGVTGYFQRGEQDQSYVPGFDASERTVAVSAAVKQN
                     QPMVRAVTDELVSRLRITVAVERNAQVEDNGDTVPADTLMRVELINSNGVQASNQVRF
                     NEKSSGTYYQDVEFDVVPQVPFNIRVFRISPDSKTDKVSNNTYFSSYVEIVDAKLSYP
                     HTAFAALSIDSAQFGNQIPRRNYLMKGRLVKVPSNYNPETRQYTGGTWDGSFKTAWTN
                     NPAWVFYDVLTQPRFSTLARRLNVADIDKWSLYQIGKYCDEPVDDGFGGKEPRFVCNA
                     YITDLMQAGEFLNNLASVFTGLPVWNGQQVSVVMDADADPVAQYTNANVKDGLFNYAG
                     AALKSIHTAVHVQYIDKYDGYRAKTEYVADNEAIARYGLNIKQITAFGCDSRGQAARF
                     GAWTLQTELRQQNAVTFEIGREGLKHLPYDIVQIMDNQYAGAELSGRVAAISGNVLTL
                     DRPVSDAVGALVFYSEGGSLKSAKITAAAGNKVTLEREVPLQAGDTWVLSGKVKPRLY
                     RAIGIKENTDAGTYTVTALLHDPKKYAAVDSFAHFDHEITTLHNTAPVLTDAAVNTDG
                     GTVVVTWDNLAANGQVLTYDIKIYRNNSLYRHIPDAQTAEIRLENLPNGNYRAEIRGR
                     NARGVLSAPLVKAWSIDYTVTGLRTTPKTLSVTVDWVLPQTVVSDLVFELWYSKTNNL
                     QTATKLASLAYPQNSYTLTGVGVADVYYFWVRIRDIAGNTGEFTAAVQGRADNNPAPI
                     VQQIQGAIGKSALSRELIDTLNRDMAAAAGLKVSAEAAERAKALQEEARARTAAIQAE
                     SSARTAELARKSSEIGTRIAATEAVNEQQAQQIQTVTSAQGNTAAGLEAEKKARTDGD
                     RAEAQARETLAVRLGNAESGISALRETVTRQDAARSSEINTLTAKLENVEVGGRNYAL
                     STGTAGKVLTASENNQTKTVTIDVSSALDLKQGDSLIISCDIELVNATSPHGKPYPRI
                     GAEFSVIYADNSVGYFAAWYGEAVSGTTKTLKQRIVAKHTVAKEVKALRSFIVQARYQ
                     TSESIKVSNVKLERGTVATDWSPAPEDSNADTSALTTLSAKVQQASSAVAEIGGKIQS
                     LYTLKTEAIAGGRRAVAGVAVGADGKTGSGEILLMADKVAYVDPKDKTVTPAFVTVIE
                     NGKAKQALNGDLFATGSILAKHIAANQTIEAPTLNGATVNAGLIKGGTIRGARIEAVD
                     LEAANIIGDVVAVRTFELKQSPLGYQELICQIPDSRKTRRTVIVPPVFAVAENGAVVS
                     LQLHMDGVIQREVTIQSPEKRIARNEKFSVWVPGPPPLYIRTPQEVPLSFSVPFHLPK
                     TGSISFQKGIDGAAHSLHILCRISGNFDYSPLGDLSGIVCLIV"
     gene            complement(4900..5622)
                     /locus_tag="WGO49_000086"
     CDS             complement(4900..5622)
                     /locus_tag="WGO49_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HEZ0305256.1"
                     /translation="MITVCLYGGLREYGRRFVLHVETPAEALHALFTQIKGLRQRIRD
                     GVYQVRFDGKDQSEETIGSVFRRPADGVLHIVPRVQGAGKNGGLIQTIVGVVLIVVGA
                     YTSWASGAQLIAVGIGMVAGGVAQMLTKQPKLDADGKGVEESKNSAFSNLANTTAQGR
                     PVPLAYGLCYCGSRVVSQGVQSRRIEPSSTAASSGAVLNLGLSKTFVQGVAATAPNGQ
                     KYRTDFSDDSVRARNYKAAFSG"
     gene            complement(5619..6374)
                     /locus_tag="WGO49_000087"
     CDS             complement(5619..6374)
                     /locus_tag="WGO49_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0305257.1"
                     /translation="MMDLDLLSEEARREMLACAEEAVPSEMCGVLVFSYEGYEFLPLS
                     NCAENPHETFEISADDWMAAERVGEIVAVVHSHPRGEPFLSGADRWMQVETGLPWILV
                     TQGRLKLFRPVPHLRGRVFEYGKTDCGALVRDAFMLMGLVFPDHPRGDMDEDAAAGFW
                     EKHLENCGFSRVSDGLCAGDVVLTATGGHASHAVLYLGNDWMLHHAYNQLSCRVPYTR
                     YWADVTHSVWRHPDFEPAMMQALENDFTHMEQA"
     gene            complement(6371..7072)
                     /locus_tag="WGO49_000088"
     CDS             complement(6371..7072)
                     /locus_tag="WGO49_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HEZ0305258.1"
                     /translation="MSGVMLQALSAAQQDVLVDLWQVDLRPLGGRVFYFCNQVNERGT
                     AVVWQGQAYEPYPIKADGFESTSQGAGNRPTLTVSNLLGLITGAADQFGQLVGVLVVR
                     RQTYAKFLDAVNFQSGRNPTADPMQEVVGKYLVERMTALNAETATFELAAPSESDGSV
                     IPARIMLANTCCWQYRGEGCGYTGRAVADRFDMPTDDASKDVCSKTLTGCRARFGATA
                     VLPFGGFPSSDKVTT"
     gene            complement(7153..7422)
                     /locus_tag="WGO49_000089"
     CDS             complement(7153..7422)
                     /locus_tag="WGO49_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0305259.1"
                     /translation="MTETFRWRVASDNKAVHKFDVRSVRFGDGYEQRQPKSLKPKLRS
                     WEIKIVGQKALMGEIKAFFDARRGVEPFNWRPPDGVPVLVKVSEY"
     gene            complement(7444..10662)
                     /locus_tag="WGO49_000090"
     CDS             complement(7444..10662)
                     /locus_tag="WGO49_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HEZ0305260.1"
                     /translation="MNNETKIYITAETGGVVSGVEKAKQSIKSLGDVASSQGRRISDG
                     LVHSGDGAEKSSTVVSAASKRTERSLASLENAIRRDIAVKIAGGKANREYYEELARQR
                     GIDIARLNPLLSQLDRHNTQTNRATQSVKQFNNALRQTPAQITDIVTQLAGGQSPFLI
                     MIQQGGQLRDMYGGFGGMLKGLATVISPMRLAVAGLGGGIAALGYAMYQGAEESCEYR
                     KALILAGDAAGITADRMQEIAVSVGAATGGYADARAAITALVSSGKVATDNYEQFARS
                     ITLQSQATGQSIDDLVEKYTEIAKDPLKAVVSLSATYRTMTADVYQQVKALQAQGREQ
                     DAVALVQRKFSEESEDMAKRVLGNLGLIERAWKDIKESASEAWDAVKSIGRDKTKLDE
                     IAALDSFISQIETSKKHPVTQLFWGEEGERKLQEAYARRARLRAEIDKEAAAAETLRQ
                     KQKQNQNRTEGIAELEAVSERYATREQQRQRELLKNDKARKKALDGITDAKKRQEIIN
                     KHAANAKLINESFAEKKHERQKEKTDKTSYPTVSNGLRIKPGAEAGGKALGGTYAFAH
                     AMQQMLGDRLFRFGAFRDRYHIGKNSLHNSGLAFDATPSPGMGMKQKQEVVAQMTRYL
                     ESLGFKNGQDFNLKLELAGQRNKNGTVATGDHWHFNWRSREAAARFAGGVEGQVKNMA
                     HLGLFKGVEKEPLSGYEKWHEDFNRRAAEAGAKRVLEILNGNRAIGEQLKLLSDPTFG
                     DWTLKQQADARELAEKADAQDSLTAASKKYSDIVKQMTDDSKEKLDDRLFEISLIGKT
                     REEIEKLTLARLWDRQIAKAREEGAPLESIDLLERGKAEGMSNLSQMQKARADSDNDW
                     RGGIESGLKSYIDSFGTMRQAMENATVQTFDKMGDALADFVATGKLDFRSLTVSILQD
                     LSKMLIKMAIVNAMKSALGYADGGIVGGGSTQFDALFSGGGYTGYGGKYEPAGIVHKG
                     EVVFSQRDVRNHGGVAAVERLRLNGYAGGGAVGLPSVLTGVRSAGAGSMQVNITINRD
                     GSTESDSSADTEMAKHLAAAIPGMVEQWYVKNVYRENGTYHK"
     gene            complement(10655..10927)
                     /locus_tag="WGO49_000091"
     CDS             complement(10655..10927)
                     /locus_tag="WGO49_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1799 domain-containing protein"
                     /protein_id="HEZ0305261.1"
                     /translation="MNVFGFSADDFSEKETTFGVWPCNWQAVQLFIGVSTQWRIGMSG
                     ATGLDYSAVAAVMECGNIKSKKRKALLEKVRLMELEVLSMWAGEHE"
     gene            complement(10975..11286)
                     /locus_tag="WGO49_000092"
     CDS             complement(10975..11286)
                     /locus_tag="WGO49_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HEZ0305262.1"
                     /translation="MAKLTLKPDATFRHTVKIPVPGAEPADVEFEFKARGRKAMKEFT
                     EKHKDGWTADTVLDCVQGWDLEEAFDRQNVEILLDSYPMAVFAIVNGYVEEVFNAREG
                     N"
     gene            complement(11350..11991)
                     /locus_tag="WGO49_000093"
     CDS             complement(11350..11991)
                     /locus_tag="WGO49_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEZ0305263.1"
                     /translation="MAVKLPNGATVHIATALAAEKKATVATNAAECVLTVAGHGFANG
                     DLVLFKSGWGKLNERVFQIGDVKADTFKLTGIDTSNADEFPAGSGIGAVQKITDWVQV
                     SQIVEFSTSGGEQQYVDFGFLEDDFDQQIPSTKSAMSMSIKIADDTSLSGYKAAAKCS
                     DKGGKWPLKVVLKGGGLICYNGYPSMNKTPELVRNQVMAVTLSYAISGEVNRY"
     gene            complement(12023..12442)
                     /locus_tag="WGO49_000094"
     CDS             complement(12023..12442)
                     /locus_tag="WGO49_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305264.1"
                     /translation="MTSRLTEIRNAATDVLKTKFQRVYSGRGFAPAQSQLPCVVVYVD
                     RRRTEQETFDFPPTYRHTVRLVTLVCVQAASGADELAEEMLSLIGQLFAEHSDLGVGD
                     LENLVPDLLNIDSDESGEAVTVYYQQGWQAIYFDTAV"
     gene            complement(12423..12725)
                     /locus_tag="WGO49_000095"
     CDS             complement(12423..12725)
                     /locus_tag="WGO49_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305265.1"
                     /translation="MFNEPLNVFTNPADFGETVMIGGKEVNAIFDREFITDSGFGVAV
                     ANADPQIIVTEDDLPEDVKSVVVTVRGKRYAVAETDFDGCGMVVVQLRAIHDKPTY"
     gene            complement(12718..12945)
                     /locus_tag="WGO49_000096"
     CDS             complement(12718..12945)
                     /locus_tag="WGO49_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305266.1"
                     /translation="MAKIKIIPTRSFFDGEQTFATGKKYSVDESAAAVYIREGWAAEA
                     PAKEAESPVADGGEAEDSGNASGEGGEDGSV"
     gene            complement(13003..14895)
                     /locus_tag="WGO49_000097"
     CDS             complement(13003..14895)
                     /locus_tag="WGO49_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEZ0305267.1"
                     /translation="MKKMKPDKAQMQQMSRFAVFQRESVDVEKRTVEVAFSSEESVRR
                     WFGDEVLSHAQGAVDLSRLNDGGAVLFNHDWDDQIGIIERAWIDADKRGRALVRFGNG
                     TKATEKFQDVQDGILRHISVGYRVEDMVLDNPDTDDEGYRYIVTRWMPYEISFVTVPA
                     DPTVGVGRSVEPFIENPVNPTPEKGNRNMDKNQTPTTVETSAAAIPAAAATDTNNTAE
                     RGMQNERARVSELLAIGRSYAAHGGIEAAEKVIKEGGSEAQLRAAIMANMQTKPTVTA
                     GEIGMTDKEQREFSLLRAMSAAATGKWDKAGLEREVSEELEKRHGRAAAGFFVPTDLI
                     ARAYSKGNAANGGNVIENDFREDLFIELLRNRLAVAQLGATVLDGLVGDITIPKHLTG
                     NTVQWVDENGSATESNATFGQMSLKPKTVTANTELSRKFILQSSLSAEQFARSELLKA
                     MMLGIDLAAINGKGTGNEPTGILNTAGIGAVEIGANGGAPEWKHIVALESAIAAANAD
                     IGDLAYITNARVRGLLKTKLKADGVSGYIWQDGATPLNGYRCAVSNQIPSNLTKGTAA
                     NKCSPLIFGNWSDLMIAHWGVLDVIVDPYTKSTTGAVRITTLQDVDIAVRHVESFAAI
                     KDIAAA"
     gene            complement(14876..16438)
                     /locus_tag="WGO49_000098"
     CDS             complement(14876..16438)
                     /locus_tag="WGO49_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEZ0305268.1"
                     /translation="MAKQSNAAPKKRGFFARLFGRREAVPKTARRNFAGARPVGSLAS
                     WQPQNWSADALARSDLDRLRTRARSLARDNDYMRKFLNMVESNVIGRDGFALQMRVLL
                     DNADKPDALANKAIEAAFSRWARRGVCDVTGQMSFTDLQRLLIRSVARDGEVLIRHIS
                     GFDNDYGYALQVLDIDRLDTGYNVPQQNGRNAVRMGVELNSYSRPVAYWLRTAHPGES
                     YGQTNTGNLRERVPAEQISHIFLHDRPEQRRGFPWVASAIIGLQNLSGYQEAAIIAAR
                     VGASKMGFFKQTEDADNFMPPIDGQEVDNGRGSIDLIDSVEPGTFHELPQGYDFTPFD
                     PDYPHANYDAFVKASLRGIASGLNVAYHSLANDLEGVNFSSIRSGTLEERDTWMTLQN
                     WFADAFLYDVFDRWIEAALLMGAIKMPSGKSLPAGKLDKFKACNWQGRRWSWVDPLKD
                     INAHKEAVALAVKSRRDICAEMGLDFEDVITQIEQENQMLAGKGIIADVKPAASAAEP
                     ESEDNPNEENEA"
     gene            complement(16447..16701)
                     /locus_tag="WGO49_000099"
     CDS             complement(16447..16701)
                     /locus_tag="WGO49_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305269.1"
                     /translation="MSGHDYAVELEEAREILNSLRKAYKEHAAGRGMTKRYKIKDREM
                     EFSSLADLLKQIRFWQQEIKRLEAAAGVSPRRSGRIITRF"
     gene            complement(16688..17074)
                     /locus_tag="WGO49_000100"
     CDS             complement(16688..17074)
                     /locus_tag="WGO49_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEZ0305270.1"
                     /translation="MEKQDNITPPHYRSRAVECIEFTERLNFCMGNAFKYVWRHREKN
                     GAEDLKKARWYLQRQLDSCAVMHLLEPEEYAELMDGLEKCELDDLMQHVLEEILYHAF
                     FESEDSLLAAMCGVSELLKGYGDERT"
     gene            complement(17077..17286)
                     /locus_tag="WGO49_000101"
     CDS             complement(17077..17286)
                     /locus_tag="WGO49_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305271.1"
                     /translation="MYDVFKTRAAELSGAFCSLDFWLCHKFVPLCHALCHFLTRRDID
                     TLIEEGLVEERFEQGVSKFYAKEAV"
     gene            complement(17303..20149)
                     /locus_tag="WGO49_000102"
     CDS             complement(17303..20149)
                     /locus_tag="WGO49_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0305272.1"
                     /translation="MNKKYATKAEINAAAHDRWREVLEACGIPSEILDKKHHPCPSCG
                     GSDRFRFTDGSGSSRGSGVWICNQCKPEGGSPFDLLMDVCGYSFNEARDKVASLLGLA
                     HGDLVDKAPKLLPPPAPVKEEERDLWKPIVPVPEYALNSMSFGNGFRKSDDLIFKSVF
                     RDSSSAVLGGVARFRKSDGGKIDLPYTFCEHKKTGEKMWRWRCWDNPRPLYGLDSLVA
                     DPSLPVLVVEGEKCKNAADAQGYNYAVLTWHGGCNNWDKSDWSAVVDRDVVLWPDCDS
                     LRQKLTKKDRDDGVDPESKPFLPRNEQGGMKAMLGIAEVLTKQNCRVWLVNIPAPGIW
                     PHGFDIADAIADGGRICDPSEVLSWMGAADWLVEYVPEAEMPSENSPAPIQDMEPESE
                     QLHESLEATGNQGGAGDVDDEYLAKLQKLKSEFGLVEGKDHGVNRKTGVTYTRKAMTV
                     HFGKDVVDAWYEWGRAPVLTMYEINRLKKDYALIQAVKDDDTQDMMERFIYLDGSASI
                     WDNKLWRVVPEKAARLSMTPDGFKIWVNSPSRIVKRFDRLVFEPGRVLSDDYINIFRG
                     MPIEPKFPIPKEDMPETNYELYRLFPEIKPILNLINHLCNGDYQQVEYILNWLAYPLQ
                     NVGAKMTTAVVMHGHIHGAGKSFFFEEIVKPMYGEYGATYGQEDLESNYTANRSGKMF
                     GIFEEVYTNQQKYNKTGSQKHMITGKTMRVERKFQDAYEEANHMNCVFLRNESHPFKI
                     EENDRRYFVVWPEKKLDKKLRDEVLDCIGNDGVRLFYSFLLCWNFYLTYGQQGAENEK
                     GYEVIKLEEPVRFDPNTPPPMTAAKQNVISYGRYAWQTFYSEWAGGGIKDLPFCCCIT
                     DDLWLVYKEWCRQNGERETGKTKFLQHIAEKMPRARRWWRTKNKDGDVSCQNWIFKPA
                     GWQAADGLPEMDALGNEVLRFRSAGWRYRGGEEV"
     gene            complement(20264..20611)
                     /locus_tag="WGO49_000103"
     CDS             complement(20264..20611)
                     /locus_tag="WGO49_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305273.1"
                     /translation="MNRTKTCKECGEIKPITAFPKNGFDRKTGLQKYAPRCRRCHNVY
                     QQDLLYKRREKEFEQKQAVMAALMDAEREKEEAAKARSIAQSLVLAKAACPILHIGLW
                     TEPLFIPQGMLQI"
     gene            21011..21727
                     /locus_tag="WGO49_000104"
     CDS             21011..21727
                     /locus_tag="WGO49_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0305274.1"
                     /translation="MTMHETTDRLFEIAKEQGVLKPADIAERLNISQQALKNWESRGI
                     AAKALPEVAKAFGVSETWLRTGEGSRTAPVLIDPDLPHEVKDIHRPMTWSSNDPLPDD
                     DYVFVPYLKESCFKGGVGTYEIPDYNGYRLPFGKSTLKRKGINPDNVFCCTLTGDSME
                     EKIAEDAAIAVDTGETAIRDGKIYAFAQDGMFRVKYLIRQPGNSVLIRSHNSGFYGDE
                     NAPLDSLTVIGRVFWWSVLD"
     gene            21830..22255
                     /locus_tag="WGO49_000105"
     CDS             21830..22255
                     /locus_tag="WGO49_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305275.1"
                     /translation="MNFLGVQGELKVHELHHRTATLCFEWLGEVSAPRRKENYKDLKP
                     NVLYDFDGSGKHFANPDARYLLPVGSSGLILKHIQIDDEDTLLRLWCARNIPMPHRLS
                     KIPMLRQYYLSKAWHEIYAINQHLRKTKLIVDVAYGPTD"
     gene            22486..22686
                     /locus_tag="WGO49_000106"
     CDS             22486..22686
                     /locus_tag="WGO49_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305276.1"
                     /translation="METITIPKENFDALLNLARTAVALNENFIPKINYGASTIPAAGF
                     AALNEFGIARSTVESLEILKIK"
     gene            22754..23113
                     /locus_tag="WGO49_000107"
     CDS             22754..23113
                     /locus_tag="WGO49_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305277.1"
                     /translation="METQEKKFVPASKQIPTVNSGWLDRELGEAMSEAVRACLAHGKQ
                     SEITVKLKIQPQNIQSGTVKISHDVATKLPKEKREGGIVFATPDGNIQADDPAQGKLK
                     LKQVPNTSSTLKMVRSN"
     gene            23138..23992
                     /locus_tag="WGO49_000108"
     CDS             23138..23992
                     /locus_tag="WGO49_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HEZ0305278.1"
                     /translation="METQENMIKTALQAAQKPFFEFAPNNTLLVFTPDQDGGWRYKSH
                     PELMQNPYRKCGKFLMHDTASLIKFVQKHKQDGTQIYIDADFKSGRIDVTAVINGHTQ
                     QAPGWLDFYAVYTPQHTTSASNWLNNNAHRMNQMEFSHFLTNNARNIVSKNPGNENSV
                     YPTAAEVLDFALNLEYTEKTTFKQGYREQDGRINFTFQSEDSGQTEKNLKMFERFGIE
                     FTPHQGGASYFVEALLKFRIDKNSGALVLWYELQQIDAVIEQAAKDISEAVQKAFPEI
                     DIYFGIIA"
     gene            24065..24280
                     /locus_tag="WGO49_000109"
     CDS             24065..24280
                     /locus_tag="WGO49_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305279.1"
                     /translation="MTITEEYSIDIRVTAEQGKNDYGYPTERYGCDIINTDGELLVGI
                     EPEYKTPFAAVRKALICLAKDNLKNAA"
     gene            24316..24576
                     /locus_tag="WGO49_000110"
     CDS             24316..24576
                     /locus_tag="WGO49_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305280.1"
                     /translation="MKYLIRTALLAVVAAGIYACQPQSEAAVQVKAENSLTAMRLAVA
                     DKQAEIDGLNAQIDAEIRQREAEELKDYRWIHGDAEVPELEK"
     gene            24573..24866
                     /locus_tag="WGO49_000111"
     CDS             24573..24866
                     /locus_tag="WGO49_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305281.1"
                     /translation="MNFQEENENIKNDIEINLLHSAEADSVEAMMDLAVYGLAAMVAK
                     HNKTRGEQLVLQAIAGHMRKLLAALPETESLVQTGKVYRRLEDLIFKTYLPEQ"
     gene            24869..25060
                     /locus_tag="WGO49_000112"
     CDS             24869..25060
                     /locus_tag="WGO49_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305282.1"
                     /translation="MNTNQDTIPFGGNLVICCSTGNGGDGRFSCLITDQIPDLNLVRS
                     GQAFPMFIYEYETREKRKA"
     gene            25060..25200
                     /locus_tag="WGO49_000113"
     CDS             25060..25200
                     /locus_tag="WGO49_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ0305283.1"
                     /translation="MDTLLKIIIALSFSGAEALAVWLFITAADAVLRRFPKPSHYRRV
                     KK"
     gene            25197..25406
                     /locus_tag="WGO49_000114"
     CDS             25197..25406
                     /locus_tag="WGO49_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEZ0305284.1"
                     /translation="MNINELGARIDRPTIRELIAYATCRNRPISNSTLLRMEKDGRIP
                     CRLKTPLTSPVWDTREVLEALGLQQ"
     gene            complement(25464..26381)
                     /locus_tag="WGO49_000115"
     CDS             complement(25464..26381)
                     /locus_tag="WGO49_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HEZ0305285.1"
                     /translation="MNVSVLNFGNTPVSFRQDGFLNATAIASHFGKLPKDYLKSEQTQ
                     QYISALAENLSVRRKILTEANQIVIVKRGGSEQGTWLHPKLAIHFARWLNPKFAVWCD
                     EQIEILLNGKISDGIKTVTPKPTRALPDGLTGEQIEAVKKLHNALTKSAPKEAQARIA
                     ITLWSAVKSKFGCSYKEVPAEQFPEVLSVMSRVAVENGVLYGEVLDREPLPAPQPALP
                     ISGNALYDLAVAVRYGAWAIQMGRDVSLPLKQLGCKQAVTMWTVWAETRSRLKAAANA
                     LEALNAHADAEHAAKIRPMLPEIRNLSSV"
     gene            complement(27249..27728)
                     /locus_tag="WGO49_000116"
     CDS             complement(27249..27728)
                     /locus_tag="WGO49_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ0305286.1"
                     /translation="MTDNLIQIATPILTVIGVFVAAYGIMRNTENAKKRATIDMIMAE
                     RNNAALQEAITIVNGLAKTDGCILATYTSDTPDKKKDREAILTVLNQREFVCAGVLGG
                     ALHEKMYKDFEYSMLLRDWDNLSSFIFEIRRIRSAPTAFQEFEAVARKWKKKPLKTK"
     gene            complement(28164..28544)
                     /locus_tag="WGO49_000117"
     CDS             complement(28164..28544)
                     /locus_tag="WGO49_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0305287.1"
                     /translation="MPLKFQPRERSVIMCDFRGYEEPEMVKKRPVVVIARNRHNGKLV
                     TVVPLSSTEPVPLADYHHKMSGNPLPDKPHIQCWAKCDMTATVGLARLDRYKPKGRDR
                     CIPIISEEDFQAIKTAVAKAFKLY"
     gene            complement(28699..29895)
                     /locus_tag="WGO49_000118"
     CDS             complement(28699..29895)
                     /locus_tag="WGO49_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HEZ0305288.1"
                     /translation="MAKIITPLSANQVKNAKPRDKLYKLSDGGGLALWVYPTGGRSWK
                     LSFVQDGRQQTISLGRYPDFSLADAREWREEVRRKRAHGENVVNKKVRADFAFEKVAR
                     DWFVRWSKGRSEKYAGQVMRNFERWVFPAIGNLDIRQVRTADVVGCLRVMEARGIVDT
                     LRKTKNSLKMVFAFAVGSGMMEINPVAQIGSGVFERAKTGNMAALSPSELPRLIDFLE
                     QRNEFAVYAGRVRIHPVTRLCIYWLLLTMTRIQEAALMEWSELDGEVWRIPAERKKER
                     RGHDVPLSRAMQWVLDQARALNVNGRFVFESVNFQGHINKESPRVAMQRAGLDTTAHG
                     LRLLARTYLREVLKVDNDVAEKLLAHSLGTRTQTAYNRSELWEERKDALERWGNDVLR
                     LAKNGK"
     gene            complement(30087..30176)
                     /locus_tag="WGO49_000119"
     tRNA            complement(30087..30176)
                     /locus_tag="WGO49_000119"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30140..30142),aa:Ser,seq:cga)
     gene            complement(30254..30344)
                     /locus_tag="WGO49_000120"
     tRNA            complement(30254..30344)
                     /locus_tag="WGO49_000120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30308..30310),aa:Ser,seq:tga)
     gene            complement(30427..31206)
                     /gene="yaaA"
                     /locus_tag="WGO49_000121"
     CDS             complement(30427..31206)
                     /gene="yaaA"
                     /locus_tag="WGO49_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ0305289.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESDILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLNTDQIQ
                     YLQGRVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRARLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHNITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            complement(31468..32655)
                     /gene="dapC"
                     /locus_tag="WGO49_000122"
     CDS             complement(31468..32655)
                     /gene="dapC"
                     /locus_tag="WGO49_000122"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ0305290.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWNDEQHVIDNRRLYQEKFEWVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAEDIVS
                     LYR"
     gene            complement(32731..33183)
                     /gene="dut"
                     /locus_tag="WGO49_000123"
     CDS             complement(32731..33183)
                     /gene="dut"
                     /locus_tag="WGO49_000123"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ0305291.1"
                     /translation="MNIEVEMKVLDERMADVVPVYATKGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVVVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            33326..>33739
                     /locus_tag="WGO49_000124"
     CDS             33326..>33739
                     /locus_tag="WGO49_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEZ0305292.1"
                     /translation="MTHISSPRNEFIRGIKESSPMLIGLLPWALILGMQGGQKGMSWL
                     EMLLMTGMNFAGGSEFATVNLWAEPLPILLIATVTFMINSRHILMGAALAPHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKAAGLPAFNMP"
BASE COUNT         8313 a         9447 c         7703 g         8276 t
ORIGIN      
        1 atttcaaagc gcgaacgcca acgggattgc gcggttacga tgcagacttt cagacggcgg
       61 tcttttgcag tgtactttaa ccagctttcg taaaatgcct caaggacttt accggcatta
      121 ttaaccttgc tttgcttcaa cactttaaac acttcatcac gggttttact tttcagatag
      181 cttccaaacg acgaattccc aaaaataagc ggaatttcgt ttggcgcaag tttgacaata
      241 cttcctgcaa taccgtctgc caacaccgcc atcggcctga tgcgctcatg gcaggtcgca
      301 atttcgtcaa acatcttgtc caccatatca acgacggttt cggtaatttg actgggctgc
      361 accttgacag ctccgtcgaa taaagcgtta ataattttaa cataggctgt acgttttgcc
      421 tgatccataa ataaacctcc ataaaaaagc ccaccctcag gcaggcagaa aaaagccgtc
      481 ctttcggacg gctaaaattt aaacaataag acagacaata cccgataaat cgcccaaggg
      541 tgaataatca aaattgccgc taatgcggca gaggatatgc aacgaatgtg ctgcaccatc
      601 gatacccttc tgaaaagata tactgcctgt ctttggtaga tggaatggga cggaaaaact
      661 aagaggcacc tcctgcggag tccttatata cagcggcgga gggccaggaa cccatacgga
      721 aaacttttcg ttacgcgcta tcctcttttc aggtgactga atcgtaacct cccgctgaat
      781 cacaccatcc atgtgcagtt gtagactgac aactgcacca ttttctgcaa cagcaaaaac
      841 cggcggcacg ataaccgtcc ggcgtgtttt tcggctatca gggatttggc atatcaattc
      901 ctgataacct aatggcgact gcttcagctc aaacgtcctg accgccacga catctccgat
      961 gatattagcg gcctccaaat ccacggcttc aattcgcgcg ccacgtatcg taccaccctt
     1021 aattagacct gcattgacgg ttgcaccatt cagcgttggg gcttcgatag tctggttggc
     1081 agcgatgtgc tttgccagta tcgaaccagt cgcgaacagg tcgccgttca aagcctgctt
     1141 cgccttgccg ttttcgataa cggtaacgaa agcgggcgta accgtcttat ctttcggatc
     1201 gacataagca actttatctg ccatcaggag gatttcgcca cttcccgtct ttccatcggc
     1261 tcccactgca acccctgcaa ctgcccgacg accgcctgct attgcctcag tcttcagcgt
     1321 gtacaaagac tgtattttcc ctcctatctc tgcaacagca gaactggcct gctgaacttt
     1381 agccgacaag gtagtcaaag cagacgtatc ggcgttactg tcctccggtg caggactcca
     1441 gtcagttgct accgtgccgc gctccagttt tacatttgaa accttgatgg attctgatgt
     1501 ctgataccgc gcctgaacga tgaagcttcg aagtgcttta acctctttgg caaccgtgtg
     1561 tttggcgaca atacgctgtt tcagcgtttt ggtcgtaccg ctgacggctt cgccgtacca
     1621 cgcagcaaaa taacctaccg agttgtccgc atagattacg gaaaattccg caccgattcg
     1681 cgggtatggt ttgccatgag gcgatgtggc gtttaccagt tcgatatcgc acgagataat
     1741 caaactatcg ccttgcttca gatccaaagc agacgaaacg tcgatagtga cggttttggt
     1801 ctgattattc tcgctagcgg tcaacacttt gccggctgtt cctgttgata aggcataatt
     1861 ccgaccgccg acttcaacat tctccagctt cgccgtcaac gtgttgatct cagacgacct
     1921 ggcagcatcc tgacgtgtta cggtttcccg caatgccgaa ataccgcttt ctgcattacc
     1981 aagtctgaca gccagtgttt cacgcgcctg cgcctcggct ctgtcgccat cggtacgggc
     2041 ttttttttcg gcttccagcc cggcggcggt attgccttgc gcagacgtaa cagtttggat
     2101 ttgctgcgcc tgctgctcat tgacagcttc tgttgcggca atacgcgtac cgatttcgga
     2161 tgactttctg gcaagctcgg cagttcgggc agatgattcc gcctgtattg ccgccgttcg
     2221 cgcccgtgct tcctcctgta aggctttagc acgttccgct gcctctgcag acactttcaa
     2281 ccccgcagcc gcagccatat cacgattcag cgtgtctatc agctccctgc ttaatgcaga
     2341 cttcccgatt gcaccctgaa tctgctgcac tatcggcgca ggattgttat ctgccctacc
     2401 ctgtacggca gcggtaaatt cccccgtatt tcctgcaata tcacgtatgc gcacccagaa
     2461 ataataaacg tcggcaacgc cgacgccggt taaggtataa ctgttttgcg gatacgccag
     2521 cgacgcgagt ttcgttgccg tctgaagatt gttggtcttg ctataccaca gctcgaaaac
     2581 caaatcggaa acaacggttt gcggcaatac ccaatccacg gtcacagaca gtgttttcgg
     2641 cgtcgtcctc aatccggtaa cggtatagtc gatactccaa gccttaacca acggtgcgga
     2701 cagtacaccc cgtgcattac ggccgcgtat ttccgcccga taattgccgt tcggcagatt
     2761 ttccaaacga atttccgctg tctgtgcatc agggatatgg cggtataggc tgttgttacg
     2821 gtaaatctta atatcgtaag ttaaaacctg accgtttgcg gccaggttgt cccatgtcac
     2881 cacaactgtc ccaccgtcgg tattgacagc cgcatcggtc aacacagggg cagtgttgtg
     2941 cagcgtggta atttcatggt caaaatgcgc aaaactatcc acggcagcat attttttcgg
     3001 gtcatgcagc aacgcggtaa cggtataagt acccgcatcc gtattctctt tgatgccgat
     3061 ggcacggtag aggcgcggct tgactttgcc ggataaaacc cacgtatcgc cagcctgcaa
     3121 cggcacctca cgctccaaag taaccttatt gcctgctgcc gcagttatct tcgctgattt
     3181 cagactaccg ccttcagaat aaaacaccaa ggctccgacc gcatcggata caggtcgatc
     3241 cagcgtcagc acattgcccg aaatggcggc aacacgtcca gacaactctg cacctgcgta
     3301 ttgattgtcc ataatctgca cgatgtcgta aggcagatgc ttcaacccct cacgcccgat
     3361 ttcaaacgtt acggcgttct gctgccgcaa ctcagtttgc agcgtccatg cgccgaaacg
     3421 tgccgcctgc ccgcgcgaat cgcagccgaa cgcagtaatc tgcttgatat tcaaaccata
     3481 gcgggcaatg gcttcgttgt cggctacata ttctgtttta gcgcgatagc catcgtattt
     3541 gtctatatac tgcacatgaa cagcagtatg aatggacttc aacgctgcac ccgcataatt
     3601 aaacaggccg tctttaacat tggcattggt atattgcgcc accggatcgg cgtcggcatc
     3661 catgacgacg gatacttgct gtccgttcca aaccggtaaa ccggtaaaga cgctggcaag
     3721 attgttcaaa aattcacctg cctgcatcag gtcggtaatg taggcgttgc agacgaaacg
     3781 cggctctttg ccgccgaagc cgtcatcgac cggctcgtcg caatatttcc cgatttggta
     3841 caggctccat ttgtctatgt ctgccacatt cagacgacgt gccagcgtgg aaaatctcgg
     3901 ctgggtcagc acatcgtaaa aaacccatgc cgggttgttt gtccatgcgg ttttgaaact
     3961 gccatcccat gttccacccg tatattggcg ggtttcagga ttgtaattgg acggcacttt
     4021 gaccaagcgg cctttcatca aataattgcg acgcggaatc tgattcccga actgcgccga
     4081 atcaatcgac aatgccgcaa acgcggtatg cggatagctc aatttcgcat caacgatttc
     4141 aacgtaggac gaaaaatagg tattgttgct gaccttgtcg gtcttggaat caggcgaaat
     4201 tcggaaaacg cggatattga acggcacttg cggcaccaca tcaaattcga catcctgata
     4261 atatgtcccg ctggattttt cattgaaacg cacctgattt gatgcctgca caccattgct
     4321 gtttatcaac tcaacgcgca ttaaggtatc agcaggcacg gtgtcgccgt tgtcttcaac
     4381 ctgtgcattc cgctcgacag cgaccgtaat ccgcaaacgg ctgaccaatt cgtcggtaac
     4441 ggcgcgaacc atcggctgat tctgcttcac cgcagccgaa acggctacgg tgcgctcgga
     4501 agcatcgaaa cccggaacat aggactggtc ttgctcgcca cgctggaaat aacccgtaac
     4561 tccattaaaa ttgtagctgc cgtccgaatt ttggacagga gtgtcatcaa aatacacgct
     4621 tttgaacggc gcatcatcgc cgttggcaaa ccccgaaacc acaccttcgg ataccgcatc
     4681 aataatccgc agcgactggg cgctggataa actgtttggg gcttcataag gtgtccgttg
     4741 tcctccgccc gatttgccgc ccatattgtt tcctttcctg attcaaaacc ttacttcacg
     4801 gtattcagaa taccgatcaa agcccaaaaa tacacaaaca aaaacagaca cgccgcgaag
     4861 tttgctcctt cgcggcgttt cgatttttca gacgacattt cagcccgaaa acgccgcctt
     4921 ataattgcgc gcccgcacgc tgtcgtctga aaagtcggta cggtatttct gcccgttcgg
     4981 cgcggttgct gctacgcctt gaacgaatgt cttggacaaa cccaaattca acaccgcacc
     5041 tgacgaagcc gccgtcgatg acggttcgat acggcgggac tgcacaccct gcgatacaac
     5101 acggctgccg cagtagcaaa gcccgtaagc caacggcaca gggcgaccct gtgccgttgt
     5161 attggcaagg ttggaaaaag cagaattctt actctcctcc acccccttgc cgtcggcatc
     5221 caatttgggc tgtttggtca acatttgggc gacaccgcca gccaccatac cgataccgac
     5281 ggcgataagc tgcgcaccac tggcccaact cgtatacgca ccgactacaa tcaaaaccac
     5341 tccgacaatg gtttggatca acccgccatt tttacctgca ccctgtacgc gcggaacaat
     5401 gtgcaataca ccgtcagcag gtcgtctgaa aacgctacct atcgtttctt ccgactggtc
     5461 tttaccgtca aaccgaacct gatacacccc gtcccgaatc cgctgacgca agcctttgat
     5521 ttgggtaaac agagcgtgca gggcttcggc tggcgtctcg acgtgcaaaa caaaacggcg
     5581 gccgtattcg cgaaggccgc cgtacagaca aacagtaatc atgcttgctc catatgggta
     5641 aaatcgttct caagtgcctg catcatggcc ggctcgaaat cgggatgccg ccaaacgctg
     5701 tgcgtaacat ccgcccaata gcgggtataa ggtacacggc agctcaattg attgtaggca
     5761 tggtgcagca tccagtcgtt gcccaaatac aggacggcat gggacgcatg cccgcccgtt
     5821 gccgtcaaga ccacatcgcc ggcgcaaagg ccgtctgaaa cacgggaaaa cccgcagttt
     5881 tccaaatgct tttcccaaaa gcccgccgct gcgtcctcat ccatatcacc gcgtggatgg
     5941 tcgggaaaaa ccagccccat cagcatgaag gcatcgcgca ccagcgcgcc gcaatccgtc
     6001 ttgccgtatt cgaatacacg cccgcgcaaa tgcggaacgg ggcggaacag tttcagacgg
     6061 ccttgtgtaa ccaaaatcca aggcaagccc gtttcgacct gcatccatct gtctgcaccc
     6121 gataaaaacg gctcgccgcg cggatgggaa tgcacgactg ccacaatctc gccgacgcgc
     6181 tccgccgcca tccagtcgtc agcggaaatc tcaaaggttt catgcggatt ttcggcgcaa
     6241 ttggacaaag gcagaaactc atagccttca taactgaaaa ccaaaacccc gcacatttca
     6301 gacggtacgg cttcttcagc acacgccagc atttcgcggc gggcttcttc acttaacaaa
     6361 tccaaatcca tcatgtcgtt accttatcac tagatgggaa accgccgaac ggcaaaactg
     6421 ccgtcgcgcc aaaccgcgcc ctacatcctg tcagtgtttt gctgcatacg tctttggacg
     6481 catcgtccgt gggcatatcg aaacggtcgg caaccgcacg ccccgtataa ccgcaccctt
     6541 cgccgcgata ctgccagcag caggtatttg ccagcataat acgggcggga atgaccgaac
     6601 catccgattc ggacggcgcg gcaagttcga aggttgccgt ttctgcattc aatgccgtca
     6661 tccgttcaac caaatatttg ccgacgactt cctgcatcgg gtctgcggtc ggattgcgcc
     6721 cggactggaa attgaccgca tctaaaaact tggcataggt ttgacggcgc acaaccagca
     6781 cgccgacgag ctgaccgaac tgatccgccg cgcccgtaat caaacccaag agattcgata
     6841 ccgtcagcgt cggacggtta cccgcgcctt gcgaagttga ttcaaagccg tcagccttaa
     6901 tcggataagg ctcatatgcc tgtccctgcc agacaaccgc cgtaccccgc tcgttgacct
     6961 gattgcagaa atagaaaacc cgaccgccca gcggccgcaa gtccacttgc cacaaatcga
     7021 ccaaaacatc ctgctgcgcg gcagacaagg cttgcagcat cacacccgac atggccttta
     7081 tacgcggatt catgaaagca cctcctcaaa cgtacagctc aattcgtagg ctttgccacc
     7141 cttcgccgtt tcctaatatt ccgatacctt gaccaataca ggcacgccgt cgggcggtct
     7201 ccaattaaac ggctcaacac cgcgccgggc atcaaaaaag gctttgattt ctcccatcaa
     7261 agccttttgt ccgactatct taatctccca gcttcgcagc ttcggtttca aagatttagg
     7321 ctgccgttgc tcgtagccgt cgccaaagcg gacggaacgg acatcgaatt tatgtacagc
     7381 cttattgtcc gaagcaacac gccatcggaa tgtttcagtc atgatcggtt cctttcagac
     7441 gacctattta tggtaagtac cgttttcacg gtacacattc ttgacatacc actgttccac
     7501 catgcccggt atggcagcgg caagatgctt cgccatctcc gtatcggcac tgctgtccga
     7561 ttcggtgcta ccgtcccgat ttatagtgat gttgacctgc atgcttccag ccccggccga
     7621 acggactccg gtcaaaacgg acggcagtcc tactgcgccg ccaccggcat aaccattcaa
     7681 ccgcagccgc tcgacagccg caacgccgcc gtgattacgt acgtcccgct gggaaaatac
     7741 gacttcgccc ttgtgaacaa tgccggcagg ctcatacttc ccgccgtagc cggtatagcc
     7801 gccgccggaa aacaacgcgt cgaactgcgt acttcctcca ccgacaatac cgccgtcggc
     7861 ataacccaac gccgacttca ttgcattgac aatcgccatt ttaatcagca tttttgacaa
     7921 atcctgcaaa atggaaacag tcaggcttcg aaaatcaagt ttgcctgtgg caacaaagtc
     7981 tgccagcgca tcccccattt tgtcgaaggt ttgtacggtg gcattttcca tcgcctgcct
     8041 catcgttccg aatgaatcga tgtagctttt caagccggac tcgataccgc cgcgccaatc
     8101 attgtcgctg tctgcacgcg ccttctgcat ttgagaaaga ttgctcatcc cttccgcctt
     8161 gccgcgctcc aataaatcga tactttccaa cggcgcacct tcttcacggg ctttcgctat
     8221 ctgtctgtcc cacagtctcg ccagcgtcag tttctcgatt tcttcacggg ttttgccgat
     8281 aagcgaaatt tcaaacagcc tgtcatccaa cttttctttc gaatcgtcgg tcatttgctt
     8341 cactatgtcg gaatattttt tggaggcggc cgtcaaacta tcctgcgcat cggccttctc
     8401 cgccagctcg cgggcatcgg cctgctgctt cagcgtccaa tcgccgaaag tcgggtcgga
     8461 caacaacttc aactgttcgc cgatggcgcg gttgccgttt aaaatttcca gcacccgctt
     8521 cgctcccgcc tccgccgccc gacggttaaa atcctcgtgc catttttcgt agccggaaag
     8581 aggttctttc tccactccct taaacaaccc gaggtgcgcc atatttttca cctggccctc
     8641 aacaccgccg gcaaatcgcg cagccgcctc tcgagaacgc cagttgaaat gccagtggtc
     8701 gccggttgct accgtaccgt ttttattgcg ctgtccggca agttccagtt taaggttaaa
     8761 atcctgcccg tttttaaacc ccaacgactc aagataacgg gtcatttggg cgacaacttc
     8821 ctgtttctgc ttcatcccca taccgggaga aggagtggca tcaaatgcca atccactgtt
     8881 atgcaggctg tttttcccaa tgtggtatcg atctcggaag gcgccgaaac ggaacagacg
     8941 gtctcccagc atctgctgca tggcatgcgc gaaggcatat gttccgccta gcgctttgcc
     9001 gcccgcttcg gctcccggtt taatcctcaa gccgttcgaa acagtggggt agcttgtttt
     9061 atccgttttt tctttctgtc gttcgtgttt tttctcggca aaactttcat taatcagctt
     9121 ggcatttgcc gcgtgcttat taatgatttc ttgccgcttt ttcgcgtcag tgatgccgtc
     9181 caacgccttt ttccgtgctt tgtcgttttt aagcagttcg cgctgccgtt gctgctctcg
     9241 agtggcataa cgttccgaaa cagcctccaa ttcggctata ccttcagttc gattttggtt
     9301 ctgcttctgt ttctgccgca aagtttcagc agcggctgcc tccttgtcga tttcggcacg
     9361 caaccgggca cgccgcgcat aggcttcctg cagcttacgc tcaccctctt caccccaaaa
     9421 caattgtgta accggatgtt tcttgctggt ttcgatttgg gaaataaacg aatctagcgc
     9481 ggcaatttca tccagcttgg tcttgtccct acctatggat ttaacagcat cccatgcctc
     9541 cgacgcactt tctttaatat ccttccacgc ccgctcaatc aacccaagat tacccagtac
     9601 gcgttttgcc atatcctccg actcttcgga gaatttccgc tgcaccaatg caaccgcgtc
     9661 ctgttcccga ccttgtgcct gcaaagcctt gacctgctgg taaacatcgg cagtcattgt
     9721 ccggtaggtg gctgataggg ataccacagc tttcagcggg tctttggcga tttcagtata
     9781 tttctcaacc aaatcgtcaa tactttgccc cgttgcctgc gactggaggg taatactccg
     9841 ggcgaactgt tcgtaattgt ccgttgcaac cttgccgctt gagaccaagg ccgtaattgc
     9901 agcacgcgca tcagcatagc cgcccgtcgc tgccccgacc gaaacagcga tttcctgcat
     9961 cctgtctgca gtaatccctg ctgcatctcc ggcaagtatc aaggctttac ggtattcgca
    10021 cgattcctct gccccctgat acatcgcata acccaatgcc gcaatgccgc cgcccaaccc
    10081 cgcgacagca aggcgcatag gggaaatgac cgtggccagt cccttaagca taccaccgaa
    10141 gccgccatac atatcacgca actgcccacc ctgttgaatc atgatcagaa acgggctttg
    10201 cccgcctgca agctgggtaa cgatgtcggt aatttgtgcc ggggtttgcc gcaatgcgtt
    10261 attgaactgc ttgacagact gcgtagcgcg attggtctgc gtattatgcc tgtccaattg
    10321 ggacagtagc ggattaagcc gtgcaatatc gataccgcgc tggcgcgcaa gctcttcgta
    10381 atactctcgg tttgccttac cgcctgcaat cttaacggct atatcgcggc ggatggcatt
    10441 ctccagagaa gccaaagacc gttccgtccg cttcgatgcc gccgacacta ccgtcgatga
    10501 tttctctgct ccgtcgccac tatgtaccaa tccgtcagaa atacgccgcc cctgggaaga
    10561 tgccacatcc cccaaagact tgatggattg ttttgccttt tctacgccgc tgacgacacc
    10621 gccagtttcg gcggtaatat aaatcttggt ttcgttattc atgttcgcct gcccacattg
    10681 acaacacttc aagctccatc aggcggactt tctccaacag tgctttccgt ttcttcgact
    10741 tgatattgcc gcattccata actgctgcga ctgcggaata atccaacccc gtcgcacccg
    10801 acattccgat acgccactgc gtcgatactc caataaacag ttgcaccgcc tgccagttgc
    10861 acggccatac gccaaaagtg gtctcctttt ctgaaaaatc gtccgccgaa aatccgaata
    10921 cattcaattc cgccgcgtca ggctgcttct cataaagcgc acgcgcggcg gcaatcagtt
    10981 tccctcgcgg gcattgaaaa cttcctcaac atagccgttg acgatggcaa acaccgccat
    11041 cggatagcta tccagcagga tttcgacatt ctgccggtcg aatgcttctt ccaaatccca
    11101 gccttgaaca caatctaaga cggtatctgc cgtccagccg tctttatgct tttcggtaaa
    11161 ttctttcatt gccttgcggc cgcgcgcctt aaattcaaat tcgacgtccg caggttctgc
    11221 accgggaaca gggattttta cggtatgtcg gaaagttgca tcaggcttca aagtgagttt
    11281 tgccatttca atactccaaa taaataaggt tgcccgagta actcggacaa ccttgatatg
    11341 ccgataaaat caataacggt ttacttcgcc ggaaatggcg taggacaacg ttaccgccat
    11401 tacttggtta cgaaccaatt cgggggtttt attcatgctg ggataaccgt tatagcaaat
    11461 cagcccacca cctttcaaaa ccactttcaa aggccacttg ccgcctttat cgctgcattt
    11521 tgcagccgcc ttgtagccgg acaacgaagt atcgtcagcg atcttaatcg acattgacat
    11581 agctgatttc gtggatggga tttgctggtc aaaatcgtcc tccaggaaac caaaatccac
    11641 atattgctgc tcgccgccac tggtcgaaaa ttctacaatt tgcgaaactt gcacccaatc
    11701 ggtaattttc tgaacagcac caataccgct gcctgccgga aactcatctg cattggaagt
    11761 atcaatgccg gtcagcttga atgtatcggc tttgacatcg ccaatctgga aaacgcgttc
    11821 attcaacttt ccccagccgc ttttaaacaa aaccaaatcg ccgttggcaa acccgtgtcc
    11881 tgctaccgtc aggacgcatt cggcagcatt agttgccacc gtagcttttt tctccgccgc
    11941 caaagcggtc gcaatgtgaa cggtcgcacc gttcggtaat ttcactgcca tactttaact
    12001 ccaaattaaa ggcaatctga aatcagactg ccgtatcaaa ataaattgcc tgccatcctt
    12061 gctgataata gacggtaacg gcctcgccgc tttcgtcgga atcgatattc agcaaatcag
    12121 gcaccagatt ttccaaatca ccaacaccca aatcggaatg ttcggcaaac aactgcccaa
    12181 tcaaagacag catctcttcc gccagttcgt ccgcaccgga agcagcctgc acgcaaacca
    12241 acgtaaccag ccgcaccgta tgccggtagg taggcggaaa atcaaacgtt tcctgttctg
    12301 ttcggcgacg gtcaacgtac accacaacac acggcaactg cgattgcgca ggcgcgaaac
    12361 cacgcccgga atacacgcgc tggaacttcg ttttcagcac atctgtcgcc gcattgcgga
    12421 tttcagtaag tcggcttgtc atgtatcgcc ctcaattgca caacaaccat tccgcagccg
    12481 tcaaaatcgg tttccgccac cgcataacgt ttgccacgca cggtaacaac caccgacttc
    12541 acgtcttccg gcaggtcgtc ttcagtaacg atgatttgcg ggtcggcatt ggcaacggca
    12601 acgccaaacc cgctatccgt tataaattca cggtcaaata tcgcgtttac ctctttaccg
    12661 ccaatcatca cggtttcacc gaaatcggca ggattggtaa acacgttcag cggctcgtta
    12721 aacactgccg tcctcgccgc cctcaccgga tgcgttaccg ctatcttcag cttcgccgcc
    12781 atcggcaaca ggggattcgg cctctttggc aggcgcttct gccgcccaac cttcgcggat
    12841 atagacagcc gccgcagatt cgtccaccga atattttttc cctgtggcaa acgtctgttc
    12901 accatcgaaa aaactacggg tcgggataat tttgattttt gccattttta atacctcaat
    12961 taataaggtc gtctgaaatt cagacgacct tgggtttcac aatcaagcag ccgcgatgtc
    13021 tttaatggcg gcaaaggatt cgacatgacg gacggcaata tccacatctt gcaacgtggt
    13081 gatgcgtacc gcgcccgtag tagacttggt gtacggatca acaatcacat ccaaaacgcc
    13141 ccagtgcgca atcatcaaat cagaccagtt accaaaaatc agcgggctgc atttgttggc
    13201 cgccgtgcct ttggtcaggt tggacggaat ttggtttgat accgcgcaac ggtagccgtt
    13261 caacggcgtt gcgccgtcct gccagatgta gccggacacg ccgtcggcct tcagcttggt
    13321 tttcagcaga ccgcggacgc gggcattggt gatgtatgcc aaatcgccga tgtcggcatt
    13381 ggcggcggca atggcacttt ccaaagcgac gatatgcttc cattcgggcg caccgccgtt
    13441 cgcaccgatt tccaccgcgc cgatgccggc ggtattcagg atgccggtcg gttcgttgcc
    13501 ggtacctttg ccgttgatgg ccgccaaatc gatacccagc atcatcgctt tcaataattc
    13561 gctgcgggcg aactgttcgg cagacagcga ggattgcaaa atgaatttgc ggctcaattc
    13621 agtattggcg gtaacggttt tcggtttcag gctcatttgt ccgaaagtgg cattcgattc
    13681 ggtcgcgctg ccgttttcat ccacccattg aacggtgttt ccggtcaggt gtttcggaat
    13741 agtgatgtcg ccgaccaagc cgtccagtac ggtggcgccc aactgggcaa cggcaagtcg
    13801 gttgcgcagc agttcgatga acaagtcttc gcggaagtcg ttttcgatga cgttgccgcc
    13861 gtttgccgca ttgcctttgc tgtaagcgcg ggcaatcaaa tcagtcggca caaagaagcc
    13921 tgccgctgcg cgaccatgtc gtttttccaa ctcttccgac acttcgcgtt ccaagcccgc
    13981 tttgtcccat ttgccggttg cggcggcaga catggcgcgg agaagggaaa attcacgctg
    14041 ttctttatca gtcatgccga tttcaccggc ggtaacggtc ggcttcgtct gcatgtttgc
    14101 catgatggcg gcgcgtaatt gtgcttcact gccgccctct ttaataacct tttcggctgc
    14161 ttcgataccg ccgtgggcgg cgtaactgcg accaatggcc agcagttcgg aaacgcgcgc
    14221 gcgttcgttc tgcataccgc gttcggcggt gttgttggta tcggttgctg cggcggcagg
    14281 gattgcagcg gcggaagttt ccaccgtagt gggcgtttga tttttatcca tgtttcgatt
    14341 tcctttttca ggggttgggt ttacagggtt ttcaataaat ggttccaccg acctgcctac
    14401 acctactgtc gggtctgcag gaacggttac aaaactgatt tcatacggca tccagcgggt
    14461 aacgatgtaa cggtagccct catcgtcagt atcgggattg tccaatacca tatcctccac
    14521 tcggtagccg acgctgatat ggcgtaggat gccgtcttgc acgtcttgga atttttccgt
    14581 tgccttcgta ccgttgccaa aacgtaccaa ggcacggccg cgcttgtcgg catcaatcca
    14641 agcacgttcg atgatgccga tttggtcgtc ccagtcatga ttgaacaaca ccgcgccgcc
    14701 gtcgttcagg cggcttaagt caacagcacc ctgcgcatga cttaatactt catcaccgaa
    14761 ccaacggcgt accgattctt cgctggaaaa cgccacttcg accgtccgtt tttcaacatc
    14821 aacactttcg cgctgaaata cggcaaagcg gctcatttgc tgcatttgcg ccttatcagg
    14881 cttcattttc ttcattcggg ttatcctccg attccggttc tgccgccgat gcggccggtt
    14941 ttacgtcggc aatgattcct tttcctgcca acatctggtt ttcctgttcg atttgggtaa
    15001 taacatcttc aaaatccaaa cccatttccg cgcaaatatc gcggcgggat ttgactgcca
    15061 gcgccaccgc ctctttatgc gcattaatat ctttcagcgg gtcaacccac gaccagcggc
    15121 gcccctgcca gttgcaggcc ttgaacttgt ccagcttgcc ggcaggcagc gatttgccgg
    15181 acggcatctt gattgcgccc attagcaacg ccgcctcaat ccagcgatcg aatacgtcat
    15241 acaagaacgc gtcggcaaac cagttttgca acgtcatcca tgtgtcgcgt tcctccagcg
    15301 tcccgctgcg gatactggaa aagttcacgc cctcaaggtc gttcgccaag ctgtgataag
    15361 ccacgttcaa accgctggcg ataccgcgca ggctggcttt gacgaatgcg tcgtaattgg
    15421 cgtgcgggta atccggatca aacggcgtaa agtcgtaacc ctgcggcagt tcgtgaaacg
    15481 tacccggctc gaccgaatcg attaaatcga tgctgccgcg cccgttatcg acctcttgcc
    15541 cgtcaatcgg cggcatgaag ttgtcggcgt cttccgtctg tttgaaaaag cccattttcg
    15601 atgcaccgac acgggcagcg ataatggccg cttcctgata gcccgacagg ttttgcaggc
    15661 cgatgatggc cgaagccacc cacggaaagc cgcgccgctg ctctgggcgg tcatgcagga
    15721 aaatatggct gatttgctct gccggcaccc gttcgcgtaa attgcccgta ttcgtctgtc
    15781 cgtatgattc gcccggatgt gccgtccgca accagtacgc tacaggacgg gaatagctgt
    15841 ttaactcaac acccatgcgc acggcgttac gcccgttttg ctgcggaacg ttgtatcccg
    15901 tatccaaacg gtcaatatcc agcacctgca acgcatagcc gtaatcattg tcaaaccctg
    15961 aaatatgacg aatcagcact tcgccgtccc gcgccacgct gcgaatcagc agccgttgca
    16021 ggtcggtaaa agacatctgg ccggtaacgt cgcacacgcc gcgccgcgcc cagcgggaaa
    16081 acgctgcttc aatggccttg ttcgccaagg catcgggctt gtcggcgtta tccagcaaaa
    16141 cacgcatttg cagggcaaaa ccgtcgcgcc cgataacgtt gctttcgacc atattcaaaa
    16201 acttgcgcat gtagtcgttg tcgcgtgcaa ggctgcgggc gcgagtgcgc aggcggtcaa
    16261 ggtctgaccg cgccaaagcg tccgccgacc agttttgcgg ctgccacgaa gccagcgacc
    16321 cgaccggacg cgcccctgca aagttgcggc gggctgtttt cgggacggct tcccgcctgc
    16381 cgaacaaacg cgcaaaaaaa ccgcgctttt tcggcgcggc atttgattgt tttgccatag
    16441 ctcatcctaa aatcgcgtga tgatacggcc tgaacggcgc ggggaaacac ccgccgccgc
    16501 ttccagccgc ttgatttcct gctgccagaa ccgtatctgc ttcaacaaat cggcaaggct
    16561 ggaaaactcc atttcgcggt ctttgatttt gtaccgcttg gtcatgcccc tgcctgccgc
    16621 atgttccttg taggcttttc gaaggctgtt cagaatctct cgcgcttctt caagttctac
    16681 cgcatagtca tgtccgctca tcgccgtatc ccttcaacag ttcggaaaca ccgcacattg
    16741 ccgcaagcag actgtcttca gattcaaaaa atgcatgata gagaatttct tccagtacat
    16801 gctgcatcag gtcatccaat tcgcattttt ccagaccgtc cattaactcc gcatattctt
    16861 cgggttccaa cagatgcatt acggcacaag aatcaagctg ccgctgcaaa taccatcgcg
    16921 cctttttcaa atcctccgcg ccgttctttt cccgatgacg ccagacatac ttaaacgcat
    16981 tacccatgca gaaattcagc cgttcggtaa attcaatgca ttccacggcg cggcttcgat
    17041 agtgcggcgg ggtgatgttg tcttgctttt ccattttcag acggcctctt ttgcataaaa
    17101 tttactgacg ccttgctcaa aacgctcttc caccaagcct tcctcaatca gcgtatcaat
    17161 atcgcggcgg gttaaaaaat ggcacagagc atggcacaat ggcacaaatt tatggcacaa
    17221 ccaaaaatcc aaactacaaa acgcgcccga caactctgcc gcacgcgttt tgaaaacatc
    17281 atacatatgt cagactcaat cgtcaaactt cttcaccgcc gcgatacctc caaccagcgc
    17341 tgcggaatcg taaaacctca ttgcccaggg catccatttc cggcaatccg tccgccgctt
    17401 gccagccagc cggcttaaaa atccagtttt gacacgatac gtcgccatct ttatttttcg
    17461 tccgccacca gcgccgcgcc cgcggcatct tctcagcaat gtgttggaga aactttgttt
    17521 taccagtttc ccgctcgccg ttttgcctgc accactcttt gtaaaccaac caaaggtcgt
    17581 ctgtaatgca acagcaaaac ggcaggtctt taataccacc gccagcccac tccgaataaa
    17641 aggtttgcca agcatatcgg ccataggaaa tgacgttttg tttcgcggca gtcatcggcg
    17701 gcggcgtgtt tggatcgaat cttacaggct cttccagttt gattacctcg tatccttttt
    17761 cgttttctgc tccctgttgc ccgtaagtca gataaaaatt ccaacacagc aggaaactgt
    17821 aaaacaaacg gacaccatcg ttgccgatac agtctaaaac ttcatcgcgc aattttttgt
    17881 ccaacttttt ctcaggccat acaacgaaat aacgccggtc gttttcctca attttgaaag
    17941 gatgagattc attacgcaaa aacacacaat tcatatggtt tgcttcttcg taagcatcct
    18001 ggaatttccg ctcgacgcgc atcgtcttgc cggtaatcat atgtttttgg ctgcctgttt
    18061 tattgtattt ttgctgattt gtgtaaacct cttcaaaaat accgaacatt ttgcctgatc
    18121 ggtttgccgt atagttgctt tccaagtcct cttgaccata tgttgcgccg tattcgccgt
    18181 acatcggctt cacgatttcc tcgaaaaaga aagatttacc cgcaccgtgt atatgaccgt
    18241 gcatgacaac ggcggtcgtc atcttcgcgc ctacgttttg taacggataa gccagccaat
    18301 tcaaaatgta ttcgacctgt tggtaatccc cattacataa gtgattaatc aaattcagaa
    18361 tcggcttgat ttcagggaat aatctataca attcgtagtt tgtttcgggc atatcctctt
    18421 tgggaatcgg gaatttcggt tcaatcggca tgcctcggaa gatgttgata tagtcgtcag
    18481 acaacacccg accaggctca aagaccaatc ggtcgaaccg ttttacaatt cggctcgggc
    18541 tattgaccca aattttaaaa ccatcaggcg tcatcgacag acgagccgcc ttttcaggga
    18601 caacgcgcca tagtttgttg tcccaaatcg atgccgaacc atcaagataa atgaaacgct
    18661 ccatcatatc ttgcgtgtca tcatctttta ccgcctgaat caatgcgtag tcttttttca
    18721 gacggttgat ttcgtacata gtcagcaccg gcgcacggcc ccattcatac caagcatcga
    18781 caacgtcttt gccgaaatga acagtcattg cctttcgggt atatgtaacc cctgtttttc
    18841 tgttcacgcc gtggtcttta ccttcaacca gcccaaattc tgatttcagc ttttgcaatt
    18901 tggccaaata ttcgtcatcc acatcccccg cacccccttg attgcctgtt gcttcgagag
    18961 attcatgcaa ctgctcagat tccggctcca tatcttgaat gggtgcggga gagttttcag
    19021 acggcatttc cgcttccggc acatactcaa ccaaccaatc cgccgcgccc atccagctca
    19081 acacttcgga agggtcgcag atgcgcccgc cgtctgcaat cgcatcggca atatcgaaac
    19141 catgaggcca tatccctgga gcaggaatat tgaccaacca aacacggcaa ttctgctttg
    19201 tcagtacctc agcaatacca agcatcgcct tcattccgcc ctgctcattg cgcggcagaa
    19261 acggtttgga ttcagggtca acaccatcat cgcgatcttt ctttgttagc ttttggcgca
    19321 gcgaatcgca atcaggccac aagacgacat cacgatcaac gaccgccgac caatccgact
    19381 tatcccagtt attacaaccg ccatgccacg tcaggacggc atagttataa ccctgagcat
    19441 ctgccgcgtt cttacatttc tccccctcga caaccaaaac cggtaatgat gggtcagcaa
    19501 ccaaagaatc aagaccgtac aatgggcgcg ggttatccca acaacgccaa cgccacatct
    19561 tttcgcctgt tttcttatgc tcgcaaaacg tataaggcag atcgattttt ccgccgtctg
    19621 attttctaaa tcgggcaaca ccgcccaaaa ccgcgctgga gctatcgcgg aaaacagatt
    19681 taaaaatcag gtcgtctgat tttctaaatc cattcccaaa gctcatagaa ttcagcgcat
    19741 attcaggcac aggaacgatt ggcttccaca aatcacgctc ctcctccttt actggagcag
    19801 gaggaggtaa cagcttcgga gccttatcta ccaaatcccc atgcgccaac ccaagcaagg
    19861 aggcaacttt gtctctagcc tcgttaaaac tatacccgca aacatccatc aacaaatcaa
    19921 acggactgcc gccttccggc ttgcattgat tgcaaatcca aacaccactc ccgcgacttg
    19981 aaccgctacc gtccgtaaac ctgaatcggt cggagccgcc gcatgacgga caggggtgat
    20041 gttttttatc taaaatttcg gacggtatgc cgcacgcttc taaaacctca cgccaccggt
    20101 catgcgcggc ggcattaatt tcggcttttg ttgcatattt tttattcatt ttcagtcatc
    20161 ctttacgttt tttaggcgta aaaaataccc acccacgcac acaagaatca ctctggagag
    20221 ggcgggctaa acactgattg cgcgattaat cagtacaggg taattaaatc tgtaacatcc
    20281 cttgagggat aaataacggc tcagtccaca agccgatgtg cagtatcgga caggctgctt
    20341 tcgccaacac gaggctttgt gcgatgcttc gcgccttagc tgcctcctct ttttcgcgct
    20401 cggcgtccat caatgccgcc atgactgcct gcttttgctc aaattctttt tcacggcgct
    20461 tgtacagcaa atcttgctga tagacgttgt ggcatcggcg gcatcggggc gcgtattttt
    20521 gcagcccggt ttttctgtca aagccgtttt tcggaaacgc cgttatcggc ttaatctctc
    20581 cgcactcctt acacgttttt gttcgattca tcgagaactc caatattaaa aattatcgtc
    20641 atcaatgcgg cttccccagc ccacagcgag ttttccacga caggcttttt ttatttgggt
    20701 tggtcgtctg gtttctttga gttcaaaaac aggtcgggat acttaagttt tattcgtgca
    20761 ggtatcccgc gcttcgtcca attgaaaacg cattgagggc tattccctgt tattcgacca
    20821 acttccgcgt aactgccgat tgattgcaac aggcgtttgt cttcattgac tcttttatcc
    20881 ataaataaac caaatgttta aatctaatgc taatattaaa cactatgttt agataaaaat
    20941 caagtcttgt gtaacaacat tttgtttaaa tatgggagaa taattaagcc aaccgcgaat
    21001 aggattaaaa atgacaatgc acgagacaac tgacagactt tttgagatag ccaaagagca
    21061 gggagtttta aagccggctg acatagcaga gcgtctgaat atcagccaac aggctttgaa
    21121 aaactgggaa agtcgtggca tagcggcaaa ggcgctgcct gaagtagcaa aagcattcgg
    21181 tgtatctgaa acatggctga gaacaggtga aggcagccga accgcgcccg tccttattga
    21241 ccccgaccta ccccacgaag tcaaagacat ccaccgcccg atgacgtgga gcagcaacga
    21301 cccgctgccc gacgatgatt atgttttcgt cccctacctc aaagagagct gcttcaaagg
    21361 cggagtaggc acgtatgaaa tccccgatta caacggctac cgcctgccgt tcggcaaatc
    21421 cacgcttaaa cgcaaaggca tcaatcccga caacgtgttt tgctgcaccc tgaccggcga
    21481 cagtatggag gaaaaaatcg cagaagacgc ggcaattgcc gtagatacgg gcgaaaccgc
    21541 catacgcgac ggcaaaatct acgccttcgc ccaggacggt atgttccgcg tgaagtacct
    21601 gatacggcag cccggcaaca gcgttctgat acgcagccac aacagcggtt tctatggcga
    21661 cgaaaacgcc cccttggaca gcctgaccgt tatcggcagg gtattttggt ggagcgtgct
    21721 ggattgaaaa atgttgctat tacgacgaga taatattgat agggctttca agattgtcaa
    21781 aaataggcgt tttgattccc cttggtggcc tggtgagtac gatgccggta tgaatttcct
    21841 tggcgtacaa ggagagttga aggttcacga gctgcatcac agaacagcaa ccctgtgctt
    21901 tgagtggctt ggcgaagtat ctgcgccgcg cagaaaagag aactacaaag acctcaagcc
    21961 aaatgtattg tatgactttg atggttccgg taagcatttt gccaaccctg acgcaaggta
    22021 tctattgccc gtcggttcaa gcggcttaat cttaaaacat atccaaatcg atgacgaaga
    22081 cactctgttg cggctatggt gcgcacgaaa tatcccaatg ccacaccggc tatctaaaat
    22141 cccgatgctt cggcagtatt atctaagcaa ggcatggcac gaaatttatg ctatcaacca
    22201 acacctaagg aaaacgaagc tgatagttga tgtggcatac ggccccacag attaaacaac
    22261 ccgcccgcat tatgcgggct tttttcatgc cccgccgaac ctgaaaacaa cacaaaaccg
    22321 acaaagccgc cttaataggc ggtttttttg cgcctgcgaa atctcaaaaa taaattcttt
    22381 taataatcaa aatgtttata aatcttaaac agattgttgc atattattaa acgctatgtt
    22441 taaatacacc catcgaaaca aacaacagcc acatggagta ccgacatgga aaccatcacc
    22501 atccctaaag aaaatttcga cgctttgcta aatctcgcaa gaaccgccgt cgccctgaac
    22561 gaaaatttta ttccaaaaat aaattacgga gcatccacca taccagcagc cggctttgcc
    22621 gcacttaacg agtttggaat agctcgctcc accgtggaat ctttggaaat tctcaaaatt
    22681 aaataacctc ctcggtattg tcatcccgtg ataccggcaa cctaactaac ccctctttaa
    22741 aggaaaatca aaaatggaaa cccaagaaaa gaaatttgtt cctgcctcca agcaaatacc
    22801 gaccgtaaat agcggctggt tagaccgtga attaggcgaa gccatgagtg aagcggtgcg
    22861 tgcctgcctt gctcacggta aacagtcaga aattactgtc aagctcaaaa tccaacccca
    22921 aaacatccaa agcggaacgg taaaaatcag ccacgatgta gcaaccaaac tgcccaaaga
    22981 aaaacgcgaa ggcggcatcg tctttgctac acctgacggc aacatccaag ccgacgatcc
    23041 ggcacaagga aaattgaaac tcaaacaagt tcccaatacg tcaagcacgt tgaaaatggt
    23101 cagatccaac taactcaaac ccaaaaggaa atctaaaatg gaaacccaag aaaacatgat
    23161 taaaaccgcc ttacaagcag ctcaaaaacc tttttttgag tttgcaccga acaatactct
    23221 gcttgtattc acaccagacc aagacggtgg ctggagatac aaatcacacc ccgaattgat
    23281 gcaaaacccg taccgcaagt gcggcaaatt cctcatgcac gataccgcca gtcttattaa
    23341 gtttgtacaa aaacacaaac aggacggcac acaaatctac attgatgccg atttcaaatc
    23401 agggcgtatt gatgtcaccg ccgtcatcaa cggccatacg caacaagctc ctggctggct
    23461 cgatttttat gccgtataca ccccgcaaca cactacatcg gcatccaact ggctgaacaa
    23521 caatgcgcac cgcatgaatc agatggagtt ttcccatttc ctgaccaaca atgcccgcaa
    23581 catcgtatcc aaaaatcccg gtaacgaaaa ttccgtttac ccgaccgccg ccgaagtatt
    23641 ggatttcgcg ctcaatctcg agtacaccga aaaaaccacc ttcaaacaag gctatcgtga
    23701 acaggacggc agaatcaact ttaccttcca atccgaagac tcagggcaaa cagagaaaaa
    23761 cctcaaaatg tttgaacgtt tcggcatcga gttcaccccg catcaaggcg gtgcatccta
    23821 ctttgttgaa gccctgttga aattccgcat cgacaaaaac agcggcgcac tcgtcctgtg
    23881 gtacgaattg cagcaaatcg atgctgtaat cgaacaagcg gcaaaagaca tttccgaagc
    23941 cgtacaaaaa gccttccctg aaatcgacat ctatttcggc ataatcgcct aaaaaaaaaa
    24001 ggtcgtctga aattgtgaac cttcagacga tctccacaaa aacccaactc cgaaagaccc
    24061 caaaatgacc atcacagaag aatactccat cgacatccgc gtaaccgcag aacaggggaa
    24121 aaacgactac ggctacccca ccgaacgcta cggatgcgac atcatcaaca cagacgggga
    24181 gctgcttgtc ggcatagagc cagagtacaa aacccccttt gccgccgtca gaaaggcgct
    24241 catctgtctg gcaaaagaca acctcaaaaa cgctgcctga tattaagccg ttacccccaa
    24301 taacgaggaa tcaaaatgaa atacttgatc cgcaccgcct tactcgcagt cgtagccgcc
    24361 ggcatctacg cctgccaacc gcaatcagaa gccgcagtgc aagtcaaggc tgaaaacagc
    24421 ctgaccgcta tgcgcttagc cgtcgccgac aaacaggcag agattgacgg gttgaacgcc
    24481 caaatcgacg ccgaaatcag acaacgcgaa gccgaagaat tgaaagacta ccgatggata
    24541 cacggcgacg cggaagtgcc ggagctggaa aaatgaactt tcaggaagaa aacgagaaca
    24601 tcaagaatga catagagatt aatctcttgc attccgctga agccgacagc gtggaggcaa
    24661 tgatggactt ggcggtttac gggctggcgg cgatggttgc caaacacaac aaaacgcgcg
    24721 gcgagcagct tgtgttacag gcgattgccg gtcatatgcg gaagcttttg gccgcgcttc
    24781 ccgaaaccga gtcattggtg cagaccggga aggtttaccg ccgacttgaa gatttgattt
    24841 ttaaaacgta tttgccggag cagtaaagat gaacacgaat caagatacga ttccatttgg
    24901 cggcaacctt gtgatttgct gttcgacggg aaacggcggg gacgggcggt tttcttgtct
    24961 gattaccgat cagataccgg atttaaatct tgtcagaagc gggcaggcgt tcccgatgtt
    25021 catttatgag tacgaaacta gagaaaaaag gaaagcctga tggacaccct attaaaaatc
    25081 atcatcgcgc tgtcattttc cggcgctgag gcattggcgg tatggctttt catcacggcc
    25141 gccgatgccg tattacgccg cttcccgaaa ccgagtcatt acaggagagt aaaaaaatga
    25201 acatcaacga actgggcgca aggatagacc gcccgaccat ccgcgaactg attgcctacg
    25261 caacctgccg caaccgcccc atttcaaact cgacactgct gcgtatggaa aaagacggac
    25321 ggataccgtg ccgtctgaaa accccactca catcccccgt atgggacacc cgcgaagttt
    25381 tggaagccct ggggttacag caataaaaaa acgcccgatt agggcgtttt cacattcaga
    25441 cggcttttgt attccctact gcatcaaacc gacgacaggt tgcggatttc gggcagcatc
    25501 gggcggattt ttgccgcgtg ttccgcgtcg gcgtgtgcgt ttaaggcttc gagggcgttt
    25561 gcggcggctt tgaggcggct gcgtgtttcc gcccagaccg tccacatcgt taccgcctgt
    25621 ttgcagccga gctgcttcag cggcagggaa acgtctctgc ccatttggat ggcccacgcg
    25681 ccgtatctga cggcaacggc gaggtcgtac agggcgttgc cgctgatggg cagggcaggt
    25741 tgcggtgcgg gtaacggttc gcggtcgagg acttcgccgt acagcacgcc gttttcaact
    25801 gccacgcggc tcatcacgct taaaacttcg gggaactgtt cggcaggtac ttctttgtag
    25861 ctgcatccga acttgctttt gacggcagac caaagggtaa tggcgatacg cgcctgcgct
    25921 tctttgggtg cggatttggt cagggcgttg tgcagttttt tgacggcttc gatttgttcg
    25981 ccggtcaagc cgtccggaag ggcgcgggtg ggtttggggg taactgtttt tatgccgtct
    26041 gaaattttgc cgttaagtaa aatttcaatc tgctcatcgc accaaaccgc aaatttcgga
    26101 ttaagccaac gggcaaagtg aatagcgagt ttgggatgca gccatgtgcc ttgctcactg
    26161 ccaccacgct tcacgataac tatttgattt gcttccgtta ggatttttct cctaacgctt
    26221 aaattctcag caagtgcaga gatatattgt tgagtttgtt cactttttag gtagtcttta
    26281 ggtaacttgc caaagtgaga tgcaatggcg gttgcattta aaaaaccgtc ttgacggaaa
    26341 gatacagggg tgttaccaaa attgagaaca gatacgttca tgatagtttc ctagtttagt
    26401 ttcgaaagac ccaaatgggt ggtcgggagg ttcgaaaacc tactagaaaa ggccggcgtt
    26461 attccccttg cgggtattgt attccgcacc ctcccgacca taggaaacta gttcggatat
    26521 atcgaaacaa acaaccaaac aagaaaactt atggacgtaa aaaattcacg ctgacggggt
    26581 gaatgccgtt ctagtagagg ttttcgacgc ctcgtgtttc gtaatatatc caatctttct
    26641 attttctgca agcataaaaa agccgccatg tggcggatag ggtcgcggtc ttcaacagca
    26701 tgtaggattt aggaatgccc aaattgggcg caggggtgtt gaaaacacgc aaacagacgc
    26761 gccgccagcc ttacgggtag gcgcaccccc acataggagt aaatcgattt gatacgtata
    26821 gacgtaaaaa attccactct atcgtgttgg atatacgctg tttgtcaggt gttttcaagc
    26881 accgtgggaa atattatata gagttatgga aatgtgtcaa gagaattagc ccaaatgggc
    26941 ggacgcgcgg ttaagaaccc acataggagg gcggacttat tcccctttcg ggtcttgtat
    27001 tcgtcgccca cgcggtcata gaaacttcct gctatcgaaa caaacaacaa ggaaagaaaa
    27061 ctatagacat gaaaaaatca cattgacgga gtgattgccg ctgtagtgtg gtttctgacg
    27121 ccacgaacag gaatataaaa caaaacccct gcacatgcaa ggggtttccc aaaaaaccgt
    27181 aggcggcaaa ctgaaaggcc gtctgaattt cagacggcct atgttgcggc ggatgttaag
    27241 ctattaagct atttggtttt cagaggcttt ttcttccatt ttcgggctac ggcttcaaat
    27301 tcttgaaagg ccgtcggtgc gctcctgata cggcgtattt caaaaatgaa gctgcttagg
    27361 ttgtcccagt cacgtaacag catggagtat tcgaaatctt tatacatttt ctcgtgcagt
    27421 gctccgccca atacgcccgc acagacaaat tcgcgctggt ttaaaactgt cagtatggct
    27481 tcacggtctt tcttcttgtc cggggtatcc gatgtatagg tggcgagtat gcatccgtct
    27541 gtttttgcca gcccgtttac tatggttatg gcttcttgaa gggcggcatt gttacgttcg
    27601 gccatgatca tgtcgatggt ggcgcgcttt ttggcgtttt ctgtattcct catgatgccg
    27661 taagcggcaa caaaaacgcc gataacagtc aatatcggcg ttgctatctg tattaggttg
    27721 tcagtcattg ccgtcccagc cgtctgaaag gcggaaatca cggctttccg aataaatatg
    27781 tcccattgtc atccccttaa gctgatgttg aaaataaatc cttgcgtcgg aatatacggc
    27841 attgcttgac tattttcaag attctgtacc tcgtgaaccg gactatacaa aaaaaccgcc
    27901 ttcgtgtaaa gcggttctta aatcgcccgt taaggcggac acaacgatag ccaaactgga
    27961 acaggaggcg gcaaaggcgg gtttgtccaa aacagccttg attgaaaggc tgattgggga
    28021 tttgtaggcg tttgcagata cgacaagggg cgcattccgc gccccatcac acgcacggac
    28081 ggcttgtcat cgcctgtcgc ccgtgcgcgg ctgcccatat ttcagaacag tcttgcaagc
    28141 ccctttaagg gaacggtttt attctagtac agtttgaatg ccttggcaac ggctgtttta
    28201 atcgcctgaa aatcctcttc actgattatt ggaatgcagc ggtcgcgccc tttgggtttg
    28261 tatcggtcta atcgtgccaa tccgactgtt gccgtcatgt cgcattttgc ccaacattgg
    28321 atgtgcggct tgtccggtaa ggggtttcca ctcattttgt ggtggtagtc cgccaaaggg
    28381 acaggttctg tgctgcttaa gggtacgacc gttaccagtt tgccgttgtg cctgtttcgc
    28441 gctatgacga cgacagggcg tttcttgacc atttccggtt cttcataacc gcgaaagtcg
    28501 cacatgataa ccgaacgttc ccttggttgg aattttaaag gcattagccg ctcctgacga
    28561 tgacaggctg ctattatagc agttcttaca caaaaaaccg ccttcgtgta aagcggttgc
    28621 aaaaaagcct tccaataaaa atgcggaaac ggttttttat ttgcgtttcc gcataataca
    28681 aacacacaca caaaaaaatc attttccgtt tttggcaagc ctcaaaacat cattccccca
    28741 ccgttccagc gcatccttac gctcctccca aagctccgag cggttgtaag ccgtttgcgt
    28801 cctcgtcccc aacgaatggg caagcagctt ttccgccaca tcattatcca ccttcagaac
    28861 ctcgcgcaaa taagtacgcg caagcaagcg caaaccgtgc gccgtcgtat ccagccccgc
    28921 ccgctgcatc gccacgcgcg gactttcctt attgatatgc ccttgaaaat tcacactttc
    28981 aaacacaaac cgcccgttca cattcaacgc ccgcgcctga tccaacaccc actgcatcgc
    29041 ccgcgacagc ggcacatcat gcccccgccg ctcctttttc cgttcggcgg ggatacgcca
    29101 aacctccccg tccaactccg accactccat caacgccgcc tcctgaatcc gcgtcattgt
    29161 caacagcagc caatagatac aaagccgcgt tacaggatgg atacgcaccc tgcccgcata
    29221 aaccgcaaat tcattgcgct gctccaaaaa atcaatcagg cgcggcaatt cagacggact
    29281 caacgccgcc atattccccg ttttcgcccg ttcaaacaca cccgaaccga tttgcgcgac
    29341 agggttgatt tccatcattc ccgaaccgac cgcaaacgca aacaccatct tcagactgtt
    29401 tttcgttttg cgcaacgtat caacgatacc gcgcgcctcc atcacacgca gacagccgac
    29461 cacatccgcc gtcctgactt gacgaatatc aagattgccg atagccggaa aaacccaccg
    29521 ctcaaaattc cgcataacct gtccggcata cttttcagac cgccccttcg accaacgcac
    29581 aaaccaatca cgcgccacct tctcaaaagc aaaatccgcc cgcaccttct tattgacgac
    29641 attttccccg tgcgcccgtt ttcggcgcac ctcctcccgc cattcccgcg catcggccag
    29701 cgaaaaatca ggataccgcc ccagcgaaat tgtctgctgc cttccatcct gcacaaacga
    29761 cagcttccaa ctccgcccgc ccgtcgggta gacccacaaa gccagcccgc ccccgtccga
    29821 caacttatac agcttatcgc gcggcttcgc atttttaacc tgatttgccg acagcggcgt
    29881 aatgattttc gccattatgg taatttccca tttatccaaa aattaccata cacattacca
    29941 taaaaaaaat gcaataagaa gatgccgcaa gattgcaaat gatttacttt gacgcgtttt
    30001 gatagggcaa accgatgatt tttatatggt aattgacaca acttgaacaa aaaagaaccg
    30061 ccctgaatca gggcggtttt gttttctggc ggaaacggtg ggattcgaac ccacggagga
    30121 tttgcaccct cagcggattt cgagtccgct gcattcagcc tctctgccac gtttccgata
    30181 atgaagtaaa accaaataaa aatgccgatg ctttatgcta cttcattttt atttggtttt
    30241 tgacctcaag gattggcgga agcggtgaga ttcgaactca cggagggcta tcaaccctcg
    30301 acggttttca agaccgttgc attaaaccgc tctgccacgc ttccatcttc ttgaagaaga
    30361 cgaataataa tgaagtttat acattttgcc aagcactttt ttcggaaaat acttaattta
    30421 ttgttttcac tttatttgtt ccgaacgcat aaacacccat tcactttcat tggaagccgc
    30481 gtcattgaat gcgtagcctt cgtaattaaa atttttcagc atttcaggat cggtaatatt
    30541 gtgttctgcc gcatagcgca ccattaatcc gcgcgcgcgc ttggcataga aactgatgat
    30601 tttatattta ccgttttttt cgtctttaaa tatcggggta atcagccgcg cccgaagtgt
    30661 tttcgtgttg acggacttga aatattcctg tgaggcaagg ttgacaagcg tattgctgcc
    30721 tgcttgggca agcgtgtcat ttaaaaggtt ggtaatgatg tctccccaaa actcatacaa
    30781 attcttcccg cgcaaattgg caaatgccgt ccccatttcc aaacggtaag gctgtatcag
    30841 gtccagtgga cgcagaaggc cgtacaggcc ggacagcagg cgtacgcgcc cttgcagata
    30901 ttgtatttga tcggtattca atgtgtttgc atccatacct tcgtaaacat cgccgttgaa
    30961 cataaagacc gcctgtttgg cgttttccgg cgtaaacggc gtgttccatt ctgcattgcg
    31021 ctgcgcgttt aagagggcaa ttttgtcgga aacgtgcatc agttcggcaa tctgttgcgg
    31081 cgcaagctcg cgcaactgct gcattagaat gtcggactct gccagcaggt cgggttgggt
    31141 aaactcgctg acaggggcag ggtctttttc attaaggttc ttcgcagggg aaaggacaaa
    31201 aaacataatc tgctcggtat tgtgtcaaac acggggattt taagtttaaa cgtgtttgcg
    31261 ggcaagccga ttaaaagtga agtatagtgg attaaattta aaccagtaca gcgttgcctc
    31321 gccttgccgt actatctgta ctgtcttcgg cttcgtcgcc ttgtcctgat ttttgttaat
    31381 ccactataaa taccgttaca gcaaattcaa ccgtagaata ttgaaaaatg ccgtctgaac
    31441 aaaagttcag acggcatttc cgattcctta ccgatacagt gaaactatgt cttccgcagc
    31501 tttgacacaa gttgcgacat cggcaaccaa agcgatacgg acataacctt ccccgggatt
    31561 gccctgttcg gtatcccgtg ccaaaaagcg tccgggtaat acttggatag cggctttttg
    31621 ccataaattg cgtgcaaatg ccaaatcgtc gccatcgggg actttcaacc agatgtaaaa
    31681 cgaggcatcc ggtaatttga cgtcaaatac ctgttgcagg ataggaataa cccattcaaa
    31741 cttttcctga tacaggcggc ggttgtcaat aacgtgctgt tcgtcattcc aggcggcaat
    31801 actggcacgc tgtacgggaa tgctcattgc gctgccgtga taggttctgt aaagcagaaa
    31861 atttttaagc agttcggcat cgccggcgac aaaaccggaa cgcaggcctg gaacattgga
    31921 ccgcttggac aaactggtga acataagcag tttttgcctg cttcgcccca actgtgcagc
    31981 ggcctgcagg cagcccaaag gcttgttgcc gtcgaaatag atttcggaat agcattcgtc
    32041 cgaggcaata atgaaaccat atttgtcctg caaatcaaaa acttctttcc atccgtccaa
    32101 atccagcacg ctgccgctgg ggttgttggg cgagcagacg aacaccagtt tggtgcgttt
    32161 ccaaacctct tcggaaatac tacgccaatc ggggttgaaa gacggcgcgg ggcaattggc
    32221 aaaatggatt tcaccgccgc ccaaaagtgt cgcaccttca taaatctgat aaaagggatt
    32281 cgggctgacg attacgggtt tgaggccgtc tgaaacaggg ttcaacacgg tttggacaaa
    32341 agaaaacaac gcctccctac ttcccaaaac cggcagaatt tcggtgtcgg aattcagcgt
    32401 caacccatca taacggcgtt ttaaccagtt cgcacacgcc tgacgcagtt caggcaaacc
    32461 ggccgtcagc ggatattttt ccaactcgtg caatgaggcg gtcagcgcat ccgtaatgac
    32521 tttcggtgtc ggatgtttcg gttcgccgat atgcagtggg acggcttcca gaccttcggg
    32581 ggcggaaatg ccctgcatcg cttcacgcag tcgggcaaag ggatagggtt tgagttggtt
    32641 gagtaaggta ttcatgattt tctccggttg tcggtatatc ggaagatatg ccgtctgaac
    32701 ctgacgtgcc cttcagacgg cattttatat tcaatgcaaa cccgtactgc cgaagccgcc
    32761 ctcaccccgg ctgcttccga caaattcctc gacacgtttg aaacgcgcct gcacgattgg
    32821 cacgacaacc atctgcgcga tacgctcaaa cggtttgaca gtaaacggtt cgctgcttct
    32881 gttccataac gacaccttca attccccttg ataatcggag tcaatcaaac cgaccaaatt
    32941 gcccaaaaca atgccgtgtt tatgccccaa gccggaacgg ggcagcaaaa cggcggcata
    33001 tgcgggattc gccaaataaa ttgccaaacc cgtcggcaca agaaacgttt cacccggctg
    33061 caaaacgact tcctcatcca aacaggcgcg caaatctaaa cctgcagaac ccttcgttgc
    33121 atagacaggg acaacatccg ccatccgttc gtccaatact ttcatttcta cttcaatatt
    33181 catcgtcttg ctcctgcata cggccggttt caaagatggg agattataca caagccgtgt
    33241 ttacgctgaa acacagcaaa taggttttgt tttgaaatac tccgtctgtg tataatgccc
    33301 gaccttctta catatcaata ttaatatgac acacataagc tcgccccgta acgaatttat
    33361 acgcggcatc aaagaaagtt cgcccatgct gatcgggctt ttgccttggg cattaatact
    33421 cggtatgcag ggtggacaaa aaggcatgag ctggctggaa atgttgttga tgaccggtat
    33481 gaacttcgcc ggcggctccg agtttgccac ggtcaacctg tgggcggaac ctctgccgat
    33541 actgcttatc gccaccgtaa cctttatgat taattctcgg catatcctga tgggggcggc
    33601 acttgccccg cacctgaaag aaataccgct gaaaaaagcc gtgcccgcac tgttttttat
    33661 gtgtgatgaa agctgggcga tggcattctc cgaaatccaa aaacggaaag cagccggttt
    33721 gcccgcattc aatatgcct
//
LOCUS       DAWFAD010000004        32086 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWFAD010000004 DAWFAD010000000
VERSION     DAWFAD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32086
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.004"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(226..549)
                     /locus_tag="WGO49_000125"
     CDS             complement(226..549)
                     /locus_tag="WGO49_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ0305293.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
     gene            complement(629..1285)
                     /locus_tag="WGO49_000126"
     CDS             complement(629..1285)
                     /locus_tag="WGO49_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ0305294.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            complement(1387..1890)
                     /locus_tag="WGO49_000127"
     CDS             complement(1387..1890)
                     /locus_tag="WGO49_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ0305295.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAV"
     gene            2074..2847
                     /locus_tag="WGO49_000128"
     CDS             2074..2847
                     /locus_tag="WGO49_000128"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ0305296.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            2854..3204
                     /gene="secG"
                     /locus_tag="WGO49_000129"
     CDS             2854..3204
                     /gene="secG"
                     /locus_tag="WGO49_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ0305297.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKYGLDFSNVQQTQQAPKPI
                     SNTEPSAPVPQQQK"
     gene            3218..3303
                     /locus_tag="WGO49_000130"
     tRNA            3218..3303
                     /locus_tag="WGO49_000130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3252..3254,aa:Leu,seq:gag)
     gene            4104..4472
                     /locus_tag="WGO49_000131"
     CDS             4104..4472
                     /locus_tag="WGO49_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0305298.1"
                     /translation="MRIFKNQWIVKFAKKHKISDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            4469..4780
                     /locus_tag="WGO49_000132"
     CDS             4469..4780
                     /locus_tag="WGO49_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ0305299.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(5550..6104)
                     /locus_tag="WGO49_000133"
     CDS             complement(5550..6104)
                     /locus_tag="WGO49_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305300.1"
                     /translation="MIIGGIGGARTQTGLRFEERTDLRTLFEEIPGYDLRKTYDNAGY
                     EVWFNGELKAYCFKKYEFYRFLKRPGYNINWKDHLSKRLLPDNGLFIIIRDTLFIIEI
                     KFQQTPGSVDEKLQTCDFKRKQYTKLVHSLGWRVEYVYVLNDWFTKPEYKDVLDYIIS
                     VNCHYQFNTIPLRWFGLPDGETNE"
     gene            complement(6101..6910)
                     /locus_tag="WGO49_000134"
     CDS             complement(6101..6910)
                     /locus_tag="WGO49_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0305301.1"
                     /translation="MIDFDKPAEEDAIYQSRLKKIVSDDLVLYNENSLNVMRKILEKY
                     PNGCFDMIFSDPPYFLSNDGFSCQNGQMVSVNKGNWDKSKGMAADLEFYEEWLRLCYA
                     LLKPNGTIWVCGTFHNIYLIGYLMQTIGYHILNNITWEKPNPPPNLSCRFFTHSTETI
                     LWAKKNKKAKHTFHYEMMKAQNDGKQMKCVWTFPPPNKAEKTFGKHPTQKPLPLLERC
                     ILSASNIGDLIFDPFMGSGTTGVAALKHGRKFCGCELEEDFFELAKKRLEK"
     gene            complement(6903..7715)
                     /locus_tag="WGO49_000135"
     CDS             complement(6903..7715)
                     /locus_tag="WGO49_000135"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEZ0305302.1"
                     /translation="MASKPFLKWAGGKHKLVPFIEHNLPTPTRKRLIEPFCGSAALSL
                     ALDFEHYLLNDINADLIGLFRILKEEKSGFIDYARSFFISENNSDSRFYELREQFNFS
                     KNLHERSALFIYLNRHAFNGLCRYNSKGVFNVPFGRYKSPYFPQQEMEGFIQKSDRVE
                     LMCGDFQMILSQTDNTDTVYCDPPYAPLSPTASFTTYAKESFNLDNQIRLAQSAQQIA
                     PDSQGVLISNHDTEFTRDIYSFAILKTIEVQRNIAAKGSSRKKVGELLAIYD"
     gene            complement(7953..10583)
                     /locus_tag="WGO49_000136"
     CDS             complement(7953..10583)
                     /locus_tag="WGO49_000136"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ0305303.1"
                     /translation="MQEHYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKLLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYADKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVDPAAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPISAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGEAVKAFAGSQDGLSKELKDLRHKLHATTAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGGEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            10850..11365
                     /locus_tag="WGO49_000137"
     CDS             10850..11365
                     /locus_tag="WGO49_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0305304.1"
                     /translation="MKIKQIVKPGLAVLAAGVLSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNNRGTFPTYDELDLMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GIDPENTSGVEGITTCQYKIIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            11372..11815
                     /locus_tag="WGO49_000138"
     CDS             11372..11815
                     /locus_tag="WGO49_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ0305305.1"
                     /translation="MPFRLFLGILCGLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            11984..14041
                     /gene="ppk1"
                     /locus_tag="WGO49_000139"
     CDS             11984..14041
                     /gene="ppk1"
                     /locus_tag="WGO49_000139"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ0305306.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELARESIHFYRRRNWTGTQKKWIEDYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPIAAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            14066..14335
                     /locus_tag="WGO49_000140"
     CDS             14066..14335
                     /locus_tag="WGO49_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305307.1"
                     /translation="MPSETFPFQTAWFYSNLNRADWNQKHTFAIPAQALPLYAPNPNP
                     PHSRRHCLSDSRTKPRIRLHGSGHISNTGIVLRKTVKSASASQYI"
     gene            complement(14304..15407)
                     /locus_tag="WGO49_000141"
     CDS             complement(14304..15407)
                     /locus_tag="WGO49_000141"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ0305308.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGADDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQSGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(15487..17043)
                     /gene="dnaA"
                     /locus_tag="WGO49_000142"
     CDS             complement(15487..17043)
                     /gene="dnaA"
                     /locus_tag="WGO49_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ0305309.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     GSEEMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRHTAEPASRPESEV
                     LAKARTDAQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFLWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            17336..17470
                     /gene="rpmH"
                     /locus_tag="WGO49_000143"
     CDS             17336..17470
                     /gene="rpmH"
                     /locus_tag="WGO49_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ0305310.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            17473..17838
                     /locus_tag="WGO49_000144"
     CDS             17473..17838
                     /locus_tag="WGO49_000144"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ0305311.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRSTAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            17903..18124
                     /gene="yidD"
                     /locus_tag="WGO49_000145"
     CDS             17903..18124
                     /gene="yidD"
                     /locus_tag="WGO49_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ0305312.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPLGGHGHDPVP"
     gene            18296..19933
                     /gene="yidC"
                     /locus_tag="WGO49_000146"
     CDS             18296..19933
                     /gene="yidC"
                     /locus_tag="WGO49_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ0305313.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGRQSVCAAGECNIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(20014..20724)
                     /locus_tag="WGO49_000147"
     CDS             complement(20014..20724)
                     /locus_tag="WGO49_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ0305314.1"
                     /translation="MSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTARA
                     HLKHLGVTTPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVADPGAN
                     LVALAHKHGFEVRPLVGPSSLLLALMASGANGQSFAFKGYLPSEKNERIQAFRALEQR
                     SRQCGETQIFIETPYRNDALLADAVENLHPETRLCTATDLTLPTQEIISQTIVQWRKR
                     KEMPNLKKRPTIFVMYAG"
     gene            complement(20786..21376)
                     /gene="maf"
                     /locus_tag="WGO49_000148"
     CDS             complement(20786..21376)
                     /gene="maf"
                     /locus_tag="WGO49_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ0305315.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLTGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAIVLLNTVTGRMRRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            21524..22027
                     /locus_tag="WGO49_000149"
     CDS             21524..22027
                     /locus_tag="WGO49_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ0305316.1"
                     /translation="MSDPNLIDLEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            22061..22240
                     /gene="rpmF"
                     /locus_tag="WGO49_000150"
     CDS             22061..22240
                     /gene="rpmF"
                     /locus_tag="WGO49_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ0305317.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            22415..22942
                     /locus_tag="WGO49_000151"
     CDS             22415..22942
                     /locus_tag="WGO49_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ0305318.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            23030..24085
                     /gene="plsX"
                     /locus_tag="WGO49_000152"
     CDS             23030..24085
                     /gene="plsX"
                     /locus_tag="WGO49_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ0305319.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            24221..24472
                     /locus_tag="WGO49_000153"
     CDS             24221..24472
                     /locus_tag="WGO49_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0305320.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            24499..24915
                     /locus_tag="WGO49_000154"
     CDS             24499..24915
                     /locus_tag="WGO49_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305321.1"
                     /translation="MKTSTVVFGGFFMADNGERIQIPVLENPDIREINHFFSVSNFEK
                     KTGVLVFRIIPEPEFGNTELTVYFEKGYYLPMIQTILEGGDIEVKNLKTENYSGNTME
                     IWGDSYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            24935..25138
                     /locus_tag="WGO49_000155"
     CDS             24935..25138
                     /locus_tag="WGO49_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305322.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            25165..26127
                     /locus_tag="WGO49_000156"
     CDS             25165..26127
                     /locus_tag="WGO49_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ0305323.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSAADKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            26174..26545
                     /locus_tag="WGO49_000157"
     CDS             26174..26545
                     /locus_tag="WGO49_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEZ0305324.1"
                     /translation="MKRQAFFKPIACAAFLSAISLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLILIDYFVPPELFVKLGQHL"
     gene            26571..27497
                     /gene="fabD"
                     /locus_tag="WGO49_000158"
     CDS             26571..27497
                     /gene="fabD"
                     /locus_tag="WGO49_000158"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ0305325.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAEAAQGEVVEAVNFN
                     SPGQIVIAGNAAAVGRAMAAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIEAH"
     gene            27636..29501
                     /gene="msbA"
                     /locus_tag="WGO49_000159"
     CDS             27636..29501
                     /gene="msbA"
                     /locus_tag="WGO49_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ0305326.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDGFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            29601..31166
                     /gene="guaA"
                     /locus_tag="WGO49_000160"
     CDS             29601..31166
                     /gene="guaA"
                     /locus_tag="WGO49_000160"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ0305327.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPILGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDDTPNTLDVWMSHGDKVSKLPTGFTVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPSWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMEKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            31228..31974
                     /gene="fabG"
                     /locus_tag="WGO49_000161"
     CDS             31228..31974
                     /gene="fabG"
                     /locus_tag="WGO49_000161"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ0305328.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         8077 a         9325 c         7727 g         6957 t
ORIGIN      
        1 gcctgcacat tgaaaatgcc gcccaagcaa accttcagtt tgccagcctc gtcctagccc
       61 tctcccacgg ggagagggga ttggggtgca ggctgccttt aaggttcagg caaattttaa
      121 cttcgttgat accgcgcttt agcttcgcag aagctgcact ttcagacgac cttttggaac
      181 accacaggta cacgcattta aggaatgccg tctgaaatgc ctgcctcaat aacgcatcat
      241 gttgccgtca atctcggccg cccatgcatc gatgccgccc tgaaggttgt acaggttttc
      301 aaaacccgcg tccgccaaat acatcgccgt atgcaggctg cggataccgt ggtggcaata
      361 caccacaagc ggcacatcat ccggcagctc gttctgccgc agcggaatca gattcatcgg
      421 gatatgcagc gcatttggca gcgaacaaac cgccgtttct tcatccgtac gcacgtccaa
      481 caaacaaaac atccgccctt cgtccatcca cgccttcaat tccgccgacc caagttgcac
      541 aatatccatc gcacccccca aaaaaaaacc aagcaaaatg ccgtctgaag ccccaaaccc
      601 gctttcagac ggcatcgcaa tccgaacatc aaaactgaaa accgccaaac ggatcggcat
      661 ccttatcatc caaatgcgcc accaaggtat cgaacagcac cttctcttca aacacatcgc
      721 ccttgcgcgt aatcaaaagc gcgcgctgaa caggcttgcg gcctacgata accaccatgc
      781 gtccgccatc tttcaactgt tctttcaaca cttcaggcac aaggtttacc gcaccgccga
      841 cataaaccgc atcaaacggc gcacctgcgg aaagttcggt caacccgttg ttttgcacat
      901 aatcgatatt gtccaaaccc aagccgtcca acaccgcttt ggcgcggttt tgctgttcgg
      961 catcgatgtc gtccgacacc acacgaccag ccaattttgc caacagcgcg gtcgcatagc
     1021 ccgaacccgt gccgatttcc aaaaccgtat cgtttttcgt cagcttcaag ccctgcgcca
     1081 gccgcgccac gactttcggc tcgagcatct tatgaccgtt ggcaagcggc agcgccatat
     1141 ccgcatacgc caaaccctgc aagtcctcat cgacaaaaag ctcgcgcgga atctccgcca
     1201 aagcgtccaa aacgtcaaaa tccaatacat cccaaggacg gatttgctgt tcgaccatat
     1261 tgaaccgcgc tttttcaaaa tccatttagt gctccgtcaa ataattgttc caacaatagc
     1321 aggcattata aaaccacact cccgccgcgc caacttcggc acgcgccgcc cgcccctttc
     1381 cctcactcaa accgcctgac gcgaagcctc aaaccgcttc tccaaaatct tcgccagctc
     1441 gcccaaatac aaagcatctg tctcatcaaa ctgcgccaaa tgttcgctgt ccgcgtccaa
     1501 cacgccgata cagcggccgt ctgaaaacag cggcacgaca atctccgaac gcgacaaaga
     1561 cgaacaggca atatggtcgg gatgcgcgtc cacatccccg acgaccaccg tcccgccctt
     1621 cgcccaagcc tgaccgcaca ccccgcgacc gaacggaatc cgcgtacacg ccaaaggccc
     1681 ctgaaacggt gccaaaacca attcgtccga acgcgtatcg accaaataaa aacccaccca
     1741 aaaccaaccg aacgcctcct tcaaaaccgc cgccgtgttc gccaaattcg ccacccaatc
     1801 cgtctcgtcc gccaccacag actcaatctg cggcaacacc tcccgataaa gcgcggcctt
     1861 gtccgaagcc gaaaaatgaa gcgcgtgcat cacatctcct atagttgcat acatatcagg
     1921 cggccattat aaaacaacct gcccgaaaca acattccaaa ccgcccccgc agccgcccgc
     1981 cccaagttgc gaacccgccg catatccact aaacttcaca ttgcaacgcg ccacacgcgg
     2041 cagacagaaa aaacacgaca cggagcaaaa aagatgtatc accaaatcgg aatgtgggat
     2101 caaaaatggg tcatcggcaa ctggaaaatg aacggccggc tccagaacaa caattcgctc
     2161 atgcaccgct tccgcatcct ccccaccgcc gaacgcgtcc tcatcggact cgccgccccg
     2221 accgtttacc tgctgcaact gcacaacgcc atgcaaatcg tcttaaacaa ccgcatcctc
     2281 acctgcgccc aagacgtgag ccgcttcccc gacaacggcg cgtacaccgg cgaagtgtcc
     2341 gccgaaatgc tcgccgacac cggcacagac atcgtcctca tcggacactc cgaacgcagc
     2401 ctttatttcg gcgaaaaaaa cgaaatccaa cgccgcaaaa tggaaaacgt cctcaacgtc
     2461 ggactcatcc cgttattgtg cgtcggcgaa agcctcgaag agcgcgaagc cggcaaagaa
     2521 cacgaagtca tcgcccatca gctttccatc ctgcaagggc tggataccaa aaacatcgcc
     2581 gtcgcctacg aacccgtctg ggcgatcggc accggcaaag tcgccaccgt cgaacagatt
     2641 gccgatatgc acgcattcat ctacaaagaa atcttgtctt tgtgcggaag cgatgttaaa
     2701 atccgcgtcc tttacggcgg aagtgtgaaa gcggacaacg cggccgacat cttcgcagta
     2761 ccttatgtgg acggcgcact cgtcggcggc gcgtcattgt cgtacgactc ctttaccgcc
     2821 atcatcagtg ccgcacaaaa tgcgtagaaa aatatggaag ccttcaaaac cctaatttgg
     2881 attgttaata taatttccgc tttggccgtc atcgtgttag tattgctcca acacggcaaa
     2941 ggcgcggatg ccggcgcgac cttcggatcg ggaagcggca gcgcgcaagg cgtattcggc
     3001 tctgccggca acgctaactt cctcagccgt tcgaccgccg ttgcagcaac atttttcttt
     3061 gcaacctgca tggctatggt gtatattcac acccacacga caaaatacgg tttggacttc
     3121 agcaacgtac aacaaactca gcaagcaccc aaacccataa gcaataccga accttctgcc
     3181 cctgttcctc agcagcagaa ataacagttt ttcaaatgcc gacatggtga aattggtaga
     3241 cacgctatct tgagggggta gtggccgtag gctgtgcgag ttcaaatctc gctgtcggca
     3301 ccaacacaca aaacgcttag aaattctaag cgtttttttc gtttaatcct gtctaatgcc
     3361 gtcaaccaat cccaatcaat acaaggattt caggctgatt gttatcctgc cgtccccctt
     3421 cctgacagtg caatcccgtc caatccgccc taattgaact aacctgattt tttacagcat
     3481 ccgaaaatac cgcgagaaaa taccgtaaaa ataaagctga acgaccacca aatcaggaat
     3541 gccaagcggg aaatagcttg cggggaatac tgacgcaccg tagggaatga gggggaaacc
     3601 gtccaagatg cagagcggtt tttttttggg tttttggaaa aaacctatac tatagtggat
     3661 taaatttaaa ccggtacggc gttgcctcgc cttgccgtac cggtttaaat ttaattcact
     3721 ataaaataag aaaaagataa aaaattggta acaaatgcgg taacaaatgg taacgaatcg
     3781 gtaacaactt ttggggtttt ccggtttttc accgtcttgg cagtgggagc gtagcggaat
     3841 gaaaagccaa aacgcacgga accgcgccta ttttgagcag gaatggcggt taaaccgctt
     3901 ggttatatac ggggaatagg aagacagcga aacgcgctaa aaacgcaatt tgagccatta
     3961 aaagcgattg attaaaaaat aatcaggtta gccgccagtg tttcaggcgg cataaacgga
     4021 gaaattgcag ggtataaaaa ggcggcttgc cgtgttgtct gtctctggta taattccaag
     4081 tatcactaat caacggctac acaatgcgga tattcaaaaa ccaatggata gtgaaatttg
     4141 ccaagaaaca taaaatcagt gattccgagc tgctggaagc ggtagagcgg gcggataacg
     4201 ggctgataga cgcagattta ggcggcggtg tgattaagca gcgcatagca aggcaaggac
     4261 aaggcagaag cggcggctat cgcagcctga tattgttcaa acaggcagat agggcatttt
     4321 tcgtttacgc ctttgccaag aacgacaggg aaaacatttc ggataaagaa ctggacgttt
     4381 accgaaaagc cgccgcatat tatctgaaat acacgcgggc agagctggcg gctttgaaag
     4441 aagacggcat tatcacggag atagaatcat gaaatacaaa agcgaggcat tagccgccat
     4501 tcatgaaatg atggaagggg cttacaacat cggcgcaatc gacaaaaaga ctatgcgcgg
     4561 ctttgacaag tcctgcctga ccgaaatcaa accgttgagc ggcggagaca tcaaggcaat
     4621 cagggagaag gaggcactat cgcaagccgc tttcgccatc tatctcaacg tgggaaaaaa
     4681 tcacgtttcg gcttgggagc ggggcgttaa aaagccgagc ggcgcggcgt tgaagctgct
     4741 gaccatcgtc aaaaacaagg gtatcgaagc cattgcgtag ccgacttggc aaacggcaaa
     4801 atcagcaagt tcacaataga cacgctgctg aatatgcctg ccaaaacagg caagaccgcc
     4861 gaactgaata tcagggcgta gccgcataaa tgcccgaccg catcaaacca agccgaaacg
     4921 gcgacggtgc agacgacata gcccgacagc aaggcacggc gcagacgggc gcgaaacccg
     4981 aaacatcccc gaccgcgagg cacggggatt ttttgagccc gttgcagggg gattggattt
     5041 aagcggcgcg ggcttgaaag caaaacgggc ggggcgcaga actgtttaaa tgccgtctga
     5101 atctcaaacg atttcagacg gcattttgaa acaacggctc aaattctcga tccccttccc
     5161 ttaacgccga cgttttttat taacgcgccc cttatttctg acactttgct cataaaccgg
     5221 cataacggtc ggcaacaacc gttttagatt ttctatacgg gcattgttta cagcatcagc
     5281 ataagacacc gtactctgga ataccgccta aaccctgcgg atagttttgg atgtggtatc
     5341 gacattgtcg gcagacttgt ctaactcgac cgttttcaaa taatctttga cagatgctgc
     5401 attcagtgtc gcggaatccg cgtattttga acaaaaggtc gggctacttt atcacaatag
     5461 cagaaagatg gaaaacggcg gggattggtg aaaggtcgtc tgaaatgata ttcagacgac
     5521 cttgaacggc tgtgttttaa atatggtatt tattcatttg tttcaccatc aggcagtcca
     5581 aaccacctta agggaatcgt gttaaattgg taatggcagt taacactgat aatataatct
     5641 aaaacatctt tatattccgg ctttgtaaac caatcattca aaacatagac atattcaacc
     5701 cgccaaccta aagagtgaac caattttgta tattgttttc tcttaaaatc gcaagtttgt
     5761 aatttctcat ctactgaacc gggagtttgt tggaatttga tctcaataat aaataaggta
     5821 tcacggatga tgataaataa gccattgtcg ggtagtaatc ttttagacag atggtctttc
     5881 caattaatat tgtatcccgg tcttttcaaa aatcggtaaa actcatattt tttaaaacaa
     5941 taagccttca attctccatt gaaccaaact tcataacccg cattatcata tgtttttctt
     6001 aagtcgtacc cgggaatttc ttcaaacaac gttcgtaagt ctgtacgttc ttcgaatctg
     6061 agtcctgttt gtgtccttgc accaccgatt ccgccaataa tcatttttct aacctttttt
     6121 ttgctaattc aaaaaaatct tcttccagtt cgcaaccaca aaatttccga ccatgtttta
     6181 aggcggcaac gcctgttgtg ccgctgccca tgaaagggtc aaaaattaaa tctccgatgt
     6241 ttgaagctga gagtatgcag cgttcaagca aggggagtgg tttttgtgtc ggatgtttgc
     6301 cgaatgtttt ttcggctttg tttggaggag gaaatgtcca aacacatttc atctgtttgc
     6361 catcattttg tgctttcatc atttcataat gaaacgtgtg tttggctttt ttatttttct
     6421 ttgcccataa gattgtttct gtcgaatggg taaaaaaacg gcaggacaaa ttagggggag
     6481 gattgggttt ttcccaagta atattgttca aaatatggta tccgatagtt tgcatcaggt
     6541 agccgattaa ataaatatta tggaatgtgc cgcaaaccca aattgtgccg tttggtttta
     6601 ataaggcgta acacagtcgg agccattctt cgtagaattc caaatctgcc gccattcctt
     6661 tggatttatc ccagttgccc ttattgacgg aaaccatttg tccgttttga caactgaagc
     6721 cgtcattgga aagaaagtaa ggcgggtctg agaaaatcat atcaaaacag ccgtttggat
     6781 atttttccaa tatcttccgc atgacgttaa gggaattttc gttgtagagg acaaggtcgt
     6841 ctgaaacgat ttttttcaaa cgactttgat aaatggcgtc ttcttcagcc ggtttgtcaa
     6901 aatcaatcat aaattgccaa taattctccg acttttttac ggctgctgcc ttttgcggca
     6961 atattgcgtt gtacttcaat tgttttcaga atggcaaaag aataaatatc ccttgtgaat
     7021 tcagtatcat gatttgaaat caaaacgcct tgggaatcgg gcgcaatttg ttgggctgac
     7081 tgggcaagac ggatttgatt gtctaaattg aaactttctt tggcataagt ggtaaatgaa
     7141 gcggttgggc ttaaaggcgc gtaaggggga tcgcaataaa ccgtatcggt attgtctgtc
     7201 tgagatagaa tcatttgaaa atctccgcac attaattcaa ctctgtcaga tttctgaatg
     7261 aaaccttcca tttcttgttg gggaaagtac ggcgatttgt agcgtccgaa cggcacattg
     7321 aatacccctt tgctgttgta acggcataaa ccgttaaaag catgacggtt caaataaata
     7381 aatagtgctg agcgttcgtg cagatttttg ctgaagttga attgttcccg caattcatag
     7441 aatcttgaat cgctattgtt ttcggaaata aagaaagagc gggcgtagtc gatgaatccg
     7501 gatttttctt cttttaggat acggaataag ccgattaagt cagcgttaat atcattgagc
     7561 agataatgtt cgaagtccaa tgcaagagac aaagcggcag aaccgcaaaa gggttcaatc
     7621 aggcgttttc gtgtaggagt gggtagatta tgttcgataa aggggacaag tttatgtttg
     7681 ccgcctgccc attttaggaa gggttttgaa gccattagga tgcagagtat aagcgtggtt
     7741 gattggcttg gaattgtgcc aaaacatcgt gaggaataca acggatttat attttgggga
     7801 cgtgtgtaga gtgagtctaa atccaataga acctttccat tccaatatgt tattggactt
     7861 atcaactcca tctgccttaa aaaggccgtc tgaaaccctg tttcccagtt ttcagacggc
     7921 ctttatccgt atggctaaac cttaagagcg gtttagacga cgatattcac caagcgtccg
     7981 ggaacgacga tgattttctt cgccggtttg ccttccatga atttcaccgc gccttcggtg
     8041 gcgagtgcgg cggcttcgag gtcggctttg gatgcgtcgg cggcaacagt gattttgccg
     8101 cgcagtttgc cgttgacttg aaccatcact tcgatttcgg atttgaccaa ggcggcttcg
     8161 tcgactgtcg gccagcctgc ttcccacagt ttcgcgccgt tcaattcgct ccacagggtt
     8221 tcgcagatgt gcggcacgat gggccacaac aggcgtacgg cggtttccaa tacttcttgg
     8281 gcgacggcgc gtccttgttc gccgccggtg tcggttttgt cgtattggtt gagcaattcc
     8341 atcacggcgg cgatggcggt gttgaactgc tggcggcggc cgtagtcgtc gctgactttg
     8401 gcagtggtcg cgtgcagttt gtggcgcagg tctttgagtt ctttagacaa accgtcttgg
     8461 ctgcctgcga acgctttgac cgcttcgcct tgcttcaagt attcgtaaac ggtacgccac
     8521 aggcggcgca ggaagcggtg tgcgccttcg acgccgctgt cgctccattc gagggactgt
     8581 tcgggcggtg cggcgaacat cataaacagg cgggcggtgt ccgcgccgta ggcgttaatc
     8641 agttcttgcg gatcgacgcc gttgtttttg gacttggaca ttttttccgt gccgctgatg
     8701 acgacgggca gcccgtcggc tttgaggacg gcggaaatgg ggcggccttt gtcgtcgaac
     8761 gtcagctcga catcggcggg gttgatccaa tctttgccgc ctttgtcgtt ttcgcggtag
     8821 taggtttcgc aaacgaccat gccttgcgtc agcaggcgtt caaacggttc gtcaacattg
     8881 actagacctt cgtcgcgcat cagtttggtg aagaaacgcg cgtacaagag gtgcaaaatc
     8941 gcgtgttcga tgccgccgat gtattggtcg accgcgcccc agtatttcgc ggcggcagga
     9001 tcgaccatgc cgtctgaaaa ttttggcgac atatagcgga agaaatacca gctcgattcc
     9061 atgaaggtgt ccatggtgtc ggtttcgcgt tttgccgcgc cgccgcagca tgggcaggca
     9121 gtttcgtaaa actcgggcat ttttgccagc ggcgaaccca tgccgtcggg tacgacgttt
     9181 tcaggcaaaa cgaccggcaa ttggtcggca gggacgggta cgtcgccgca ttgttcgcaa
     9241 tggacgatgg gaatcgggca gccccagtag cgttggcgcg aaatgcccca gtcgcgcagg
     9301 cggtattggg ttttcggttc gcccgcgcct tggctttgca gcttggcggc aacggcatcg
     9361 aatgccgtct gaaaatccaa gccgtccaag tcgccgctgt tgaccaatac gccgttttct
     9421 ttgtcggcat accattcttg ccatcggttt gcgtcgaatg cgttgtcgcc gacggcaatg
     9481 acttgttttt taggcagatt gtatttggcg gcgaactcaa aatcgcgttc gtcgtgcgcc
     9541 ggaaccgcca tcactgcgcc gtcgccgtag ccccacaata catagttggc aatccacact
     9601 tccagcttgt cgccgttgag cgggttgacg acgtagcgac cggtcggcac gccttttttc
     9661 tccatcgtcg ccatatcggc ttcggcaacc gaaccggctt tgcattcggc aataaatgcc
     9721 tgcaattcgg gtttgtcggc ggctgcggcg gctgccagcg gatgctcggc ggcaacggca
     9781 acataagtcg cacccatcag cgtgtcgggg cgggtggtat aaacttgcag caatttcgcg
     9841 taatcgcctt ccaagccttg tttgctgtcg tctgaaacgg cgaagcgcac ggtcataccg
     9901 cgcgatttgc cgatccagtt gcgctgcatg gttttgactt gttccggcca gtgttccagc
     9961 ttgtccaagt cgttgagcag ctcttcggcg taatccgtga ttttgaagta atacatcggg
    10021 atttcgcgtt tttcgatcaa cgcgcccgaa cgccagccgc gtccgtcgat gacttgctcg
    10081 ttggcaagga cggtttggtc gaccgggtcc cagttcaccg tgccgttttt gcgatagacg
    10141 atgccttttt caaacagctt ggtaaacagc cattgttccc agcggtagta ttcgggtttg
    10201 caggttgcgg tttcgcgcgc ccagtcaatc gcaaaaccca ggcttttgag ctgggttttc
    10261 atgtattcga tgttatcgta cgtccaagcg gcaggggcga cgttgttttt catcgccgcg
    10321 ttttccgccg gcatgccgaa cgcgtcccaa cccataggct gcatgacgtt gaagccgttt
    10381 aaaagtttga agcggctcaa tacatcgccg atggtgtagt tgcgcacatg ccccatgtgc
    10441 agcttgccgc tgggataggg gaacatggag aggcaataat atttgggttt ggaagcgtct
    10501 tcggagacgt tgaaaatacg ggcgtcgtcc cattttttct gcgccgcagg ctcgatggcg
    10561 gcgggttggt aatgttcttg catggtcatt ctgtttttcg cttgaaaacg ttaggaattt
    10621 aaaggaggaa ttataacagg ttgccgatgt cggcgaatcg gcaaattgcc ggcaggatgc
    10681 gtaaattcgc acgcgcatca ttccgtatgc cgtacaaata caccgcgttt actgatacgc
    10741 acgtttttta tgctaatatt acaaaccaaa atcaaatgtt taaaactctc ctgatgcggc
    10801 tcttccgaac aaaaggcaga cgggcatcgg gtaaaagagg attctgcata tgaaaatcaa
    10861 acaaatcgtc aaaccgggct tggcagtatt ggcggcgggc gttctgtctg cctgcgcaac
    10921 caaaagcaac gtcaaagccg acggaacgac cgacaatccg gttttcccga aaccctattc
    10981 cgtaacgctc gacaacaatc gcggtacatt cccgacctat gacgaattgg acttgatgcg
    11041 tcccggtctg accaaagacg acatctacaa aatcctgggt cgtccgcatt acgacgaagg
    11101 tatgtacggc gtgcgcgaat gggattatct gttccacttc cacaccccgg gcgtaggcat
    11161 cgaccctgaa aacacttccg gcgtagaagg cattaccacc tgtcaataca aaattatttt
    11221 cgataaagac aaatttgccc gcagcttcta ctggaacccc gtcttcccga aagatgccgc
    11281 ctgtccgccg cccgcaccca aagccgagcc gcaagtcatc atccgcgaaa tcgtgccggc
    11341 aaaaccgaaa cgtatccgcc aataatccga catgccgttc cgcctgtttt tagggatatt
    11401 atgcggcctg tcaatggttg cccccgtata tgcacagggg caaccggatg cggtcaacgc
    11461 ttatattcaa aagaaaaaag tcatcgtaaa cacatccaaa gcggaactct gttttgccga
    11521 tgacaggcag tgccaccccg tcctcatcgg tgttgccacg cccaagggga cgttcgggct
    11581 gacgctgaac agtaccgaca agcctggata cggcggcgaa gtcatcggtt tcaagcagga
    11641 gggtgatttt cttttcgccc tgcaccgcgt ttggaatcag ataccgtcgg aaaggcggaa
    11701 cgaacgcatc gcctccccgt ccgtgtccga caggattatg accaacggct gcatcaacgt
    11761 cagcgatgcg gtgtacgaaa aactgcgtca ttattttgtg ttggaagtga tttgaaacag
    11821 acggataccg cacgcgccgg tatctgtttt cacattgccc cgatgcctga aacagactgt
    11881 ccgccacgtc atgccgtctg aaaccggcgc agatgccgcc aagccttcag acggcattgc
    11941 ctgcccgctc cgaccgaaca acaaccatct ttgggagaac cttatgcccg aacaaaaccg
    12001 catcctctgc cgcgaactaa gcctgctggc attcaaccgc cgcgtgttgg cacaggcgga
    12061 agacaaaaac gtcccccttt tggaacgcct gcgcttcctg tgcatcgtgt cgtccaatct
    12121 tgacgagttt ttcgaagtcc gcatggcgtg gctcaagcgt gaaaacaagc tgcacccccg
    12181 gcgcaggctg gacaacggca aaatgccgtc tgaaaccatc gccgacgtta ccgaagcggc
    12241 gcgctccctg atacggcatc agtacgacct gttcaacaac gtccttcagc ccgagttggc
    12301 gcgggaaagc atccattttt accgccgccg caactggacg ggcacgcaga aaaaatggat
    12361 cgaagactat ttcgaccgcg aactgctgcc gatcctgacc cccatcggac tcgacccgtc
    12421 ccaccccttc ccgcgaccgc tgaacaaatc gctcaacttc gccgtcgaac tcgacggcac
    12481 ggacgcattc ggcaggcctt cggggatggc gattgtgcag gcaccgcgca tcctgccgcg
    12541 cgtcgttccc ctgccgtccg aactgtgtgg cggcggacac ggcttcgtct tcctgtcgtc
    12601 catcctccac gcccacgtcg gcaaactctt cccgggcatg aacgtcaaag gctgccacca
    12661 gttccgcctg acgcgcgaca gcgacttgac cgttgacgaa gaagacctgc aaaacctccg
    12721 cgccgccatc caaaacgaac tgcacgaccg cgaatacggc gacggcgtgc ggctcgaagt
    12781 cgccgacacc tgtcccgcct acatccgcga ctttctgctc gcgcaattca aactgaccgc
    12841 cgccgaactc tatcaggtca aaggcccggt caacctcgtg cgcctcaacg ccgtccccga
    12901 cctagtcaac cgccccgatt tgaaatttcc cacacacacg ccgggcagac tgaaagcctt
    12961 gggcaaaacc gcgtccatat tcgatttggt gcgccaatcg cccatcctgc tgcaccaccc
    13021 ctaccaatcg ttcgaccccg ttgtcgaaat gatgcgcgaa gctgccgccg accccgacgt
    13081 gcttgccgtc aaaatgacga tttaccgcac cggcacgcgt tccgaactcg tccgcgccct
    13141 gatgaaggcg gcactcgccg gcaaacaagt aaccgtcgtc gtcgaactga tggcgcgttt
    13201 tgacgaagcc aacaacgtca actgggcgaa gcagctcgaa gaggcgggcg cgcacgtcgt
    13261 gtacggcgtg ttcggctaca aagtccacgc caagatggca ctggtcatcc gccgcgaaga
    13321 cggcgtactc aagcgttacg cccacctcgg cacgggtaac taccaccaag gcacatcgcg
    13381 catctacacc gacttcggca tcattaccgc cgacgaacaa atcaccgccg atgtgaacac
    13441 attgtttatg gaaatcacag gcttgggcaa acccgggcgg ctgaacaaac tctaccaaag
    13501 cccgtttacc ctgcacaaaa tggttatcga ccgcatcgca cgcgaaaccg aacacgcaaa
    13561 agccggcaaa ccggcgcgga ttaccgccaa gatgaattcg ctcatcgaac cgaccgtcat
    13621 cgaagccctg tatcgggcaa gcgcggcagg cgtacaaatc gatttgattg tgcgcggtat
    13681 gtgcaccttg cgcccgggtg taaaaggctt gtccgaaaac atccgcgtcc gctccatcat
    13741 cggcaggcag ctcgaacacg cgcgcgtgta ttgcttccac aacaacggcg cggacgatac
    13801 ctttatctcc agcgcggatt ggatggggcg caacttcttc cgccgcatcg aaaccgccgc
    13861 gccgattgcc gcgcccgaac tcaaaaaacg cgtcatccgc gaaggtttgg aaatggcact
    13921 ggcagacaat acgcacgcgt ggctgatgca gcccgacggc ggctatatcc gcgccgcgcc
    13981 tgccgagggc gaaagcgaag ccgacctgca aaacgatttg tggacactgc tcggaggctg
    14041 acccgcaccg ccccaatcaa aaaccatgcc gtctgaaacc tttccgtttc agacggcatg
    14101 gttttacagc aatctaaaca gggcggactg gaatcaaaaa cacaccttcg ccattcccgc
    14161 acaagcactt cccctatacg ctcccaaccc caatccgccg cattccagac ggcattgcct
    14221 ttccgacagc cgaacaaaac cccgaatccg tctgcacggt tcggggcata tctccaatac
    14281 gggcatcgtg ctccggaaaa ccgtcaaatc cgcatcggca tcacaatata tttgaaattc
    14341 ggattgttcg gcacggtaaa cagcgtcgag cggttggcat cgccgaaggc aagctgcata
    14401 tcgtcggaat ggatgttgcg caacacgtcc atcagatagc cgatattgaa accgacttcg
    14461 agttcgccgc cctgataggc aatctcgatt tcttcgcgcg cttcttcctg ctcgttgttg
    14521 ctgcacacga cgctcaacag gccggattgc aggaacaggc gcgcgccacg gaatttttcg
    14581 ttggcaagaa tggacacacg ttccagcgcg cccaaaagtt cggcacggga caaaacgaaa
    14641 atcttgtcgt tgtccaaagg gatgacgcgg ttgaaatcgg ggaatttgcc gtcgataacc
    14701 ttgctgacga tggtcgtgcc gttgcattgg aaacgcacct gcttgtccag cagctcgatt
    14761 tgaatcggat cgtcggggtt gttcaacagt ttgaacagtt ccagcaccgt tttgcgcggc
    14821 aaaatcactt cggcgcgcgg caaatccgca tcaatcgcgc aggctgcata ggcaaggcgg
    14881 tgtccgtcgg tcgccacaag gcgcaactgg ctgccctcaa cctgcatcag cagaccgttg
    14941 agataatagc ggatgtcctg caccgccatg ctgtactgca cttgcgacag catggttttg
    15001 aaacgctcct gctccagcga gaaagtcgcg ctgatgtcct cgccgacatt catcatcgga
    15061 aaatcggcgg caggcagggt ctgcagggca aaacgcgatt tgcccgcctt cagcgtcaga
    15121 cggctgtcgt cccaatccag cgacaccagc gcaccggcag gcagcgcgcg caaaatgtcc
    15181 tgaaatttct tggcattggt ggtgatgcgg aagtcgtccg cgccgccctc gggacccgca
    15241 gtgtcgattt ggatttccaa atcggttgcc aagagtttgg tctgaccgcc ttttccctca
    15301 atcaggacgt tggacaggat gggcagggtg tggcggcgtt cgacgatgcc ggtaacggct
    15361 tgcagcggct tgagcaggct gtcgcgttcg gcttgtaaaa tcaacatgtt cgctccttta
    15421 aatcggttgg attttttacc cttcggacgg catgggcaat atcatgccgt ctgaaaacgt
    15481 tttccatcag ttttgaatca gaatcagcag cttttcataa tcctgagcca attccggatc
    15541 ttcttcgcgc agtttcgcca ctgccctgat gccgtgcata acggtcgtat ggtcgcgccc
    15601 accgaacgaa tcgccgatag acggcaggct caaagtggtc agttctttgg tcaggctcat
    15661 cgccacctgg cgcggacggg caatgttgcg cgtgcgtttt ttgccgagta cgtcgctgat
    15721 tttgatgcgg taatatttgg caacggcatc aataatgata tcggcggtaa tgaccttatg
    15781 cttctcggca ataatgtcct gcaaagcggt acgcgccaaa tcgatgtcga tgacgggacg
    15841 gttcataaag cggctgctcg ctccgacacg gttaaacgcg ccttcaagct cgcgcacgtt
    15901 ggaacggatc agattggcaa tgaacagcgc ggcttcgtct tcaatactga tgcccgccgc
    15961 ttccgccttt ttctgcaaaa tggcgatgcg catttccaat tcgggcggct cgagttccaa
    16021 agtcagtccc cataaaaaac gggatttgag gcggtcgtcc atgccttcga ttttggcggg
    16081 taaaacatcg caagtgagga tcagctgttt tttctcgttg tggaaatggt tgtacagata
    16141 gaaaaactct tccatcgtac ggtcttttcc tttaataaac tgaatatcgt cgataatcag
    16201 caggtcgtat tgcttgtatt gctgcttgaa tacgtcatag gtattgttgc gaaccgcctt
    16261 cataaagctg cggatatagt cgtccgaatg catatagcgc actttggcat cgggacggtt
    16321 tttcaaaagc tcgttgccga ccgcctgtac caggtgggtt ttgcccaaac ccgtgctgcc
    16381 gtacaggaaa aacgggttgt agctctgccc cgggctttcc gcaatcgcct gcgccgcagc
    16441 cgccgcaagg cggttgccct tgccttcgac caatgtgtcg aacgtatagt ccggcgacag
    16501 attggtctgc tcgtaacgtg cctcttccgc atcgcgctgc gcatccgtcc gcgctttcgc
    16561 aagcacctcc gattccggac gggaagcagg ttcggcagta tggcgcggct catgcgggag
    16621 gtttttcata cgttccgcca gaatatccgc cgccgttttc gacgcagcgg gtttgacagg
    16681 ctcttcagac ggcagctcgt ccaacagaac ctcctgcaca ggcatctcct ccgaccccgc
    16741 gtgcaaggac ggctcggcag gttcgacagc accttcaacc gccgccatct cataacgcac
    16801 gccttctcct ggtttgaata cgaaggcgga acggccagca gccaactctt ccctcaccgc
    16861 ttctattttc ccggcaaact ggctcttaag catattgcag gcaaactggt tcttgccgta
    16921 caccacccat acgccaccct cctcaccgac ggtaaggggc gcaatccatt gcgcaaactg
    16981 cccttgaggc aacatatcgt gaagacggcg gaggcacagc ggccaaaact ctgctaatgt
    17041 catggataag ctcgaatcgg taaaaatgaa atcgaaaaca aagaaaatat aatattttca
    17101 aaaagaaaac aaatctgttg aacacacatc ggttcaaaat gcagcgaacc gaattatacc
    17161 gactcgccga tattttatcc acaacccgtg caaaatttat ccacagaaag gcggcagaaa
    17221 tccgcaggca atcgggcaat cctcctgcaa agtttctata ttgattgaca aaagcggcaa
    17281 attggagtgt aattcacggt ttaattatct acccattcta tttttaggaa acatcatgaa
    17341 acgcacttat caaccttccg ttaccaaacg caaacgcacc cacggcttcc tggtgcgctc
    17401 caaaacacgc ggcggccgcg cagtattggc tgcacgccgc gccaaaggcc gcaaacgcct
    17461 ggcggtataa ttttggacta ccgcttcgga aggcagtacc gcttgttgaa aacggatgat
    17521 ttttcatccg tttttgcatt cagaaaccgc cgcagccgcg acctgctgca agtttcgcgt
    17581 tcaaacggca acgggctgga ccatccccgc atcggtctgg tggtcggcaa aaaaaccgcc
    17641 aaacgcgcca acgaacgaaa ttatatgaag cgcgtcatcc gcgactggtt cagattgaac
    17701 aaaaaccggc tgccgccgca ggatttcgtc gtgcgcgtcc accgtaaatt cgacaggtct
    17761 accgcaaaac aggcaagggc ggaactggca caactcatgt tcggcaaccc cgcaaccgga
    17821 tgcaggaaac aggcatgatc agaacggtac tctgcaggca aggttcagac agcaacgggt
    17881 ttcccataca aggaacatcc cgatgaactt cctattgtcc aaactcctgc tgggactgat
    17941 acggttctac caatattgca tcagcccgct gattccgccg cgctgccgct atacgccgac
    18001 ctgttcgcaa tacgccgtcg aagcagtcaa aaaatacggc gcattcaaag gcggccggct
    18061 cgccatcaag cgcattgcac gctgccaccc cctcggcgga cacggacacg accccgttcc
    18121 ctgacccgac gcaatattca aattgcacgc tttcctttta tttcccatcg gtttctatat
    18181 aatgccgtct gaagcttcgg gcaggcggca cgaccgtcgg gtatgaaacc cgcccttatt
    18241 ccccgtctat cggaacacgc aacctgcggc atttccgacc attcaggaaa ctcttatgga
    18301 ttttaaaaga ctcacggcgt ttttcgccat cgcgctggtg attatgatcg gctgggaaaa
    18361 gatgttcccc actccgaagc ccgtccccgc gccccaacag gcagcacaac aacaggccgt
    18421 aaccgcttcc gccgaagccg cgctcgcgcc cgcaacgccg attaccgtaa cgaccgacac
    18481 ggttcaagcc gtcattgatg aaaaaagcgg cgacctgcgc cggctgaccc tgctcaaata
    18541 caaagcaacc ggcgacgaaa ataaaccgtt catcctgttt ggcgacggca aagaatacac
    18601 ctacgtcgcc caatccgaac ttttggacgc gcagggcaac aacattctaa aaggcatcgg
    18661 ctttagcgca ccgaaaaaac agtacagctt ggaaggcgac aaagttgaag tccgcctgag
    18721 cgcgcctgaa acacgcggtc tgaaaatcga caaagtttat actttcacca aaggcagcta
    18781 tctggtcaac gtccgcttcg acatcgccaa cggcagcggt caaaccgcca acctgagcgc
    18841 ggactaccgc atcgtccgcg accacagcga acccgagggt caaggttact ttacccactc
    18901 ttacgtcggc cctgttgttt atacccctga aggcaacttc caaaaagtca gcttttccga
    18961 cttggacgac gatgccaaat ccggcaaatc cgaggccgaa tacatccgca aaaccccgac
    19021 cggctggctc ggcatgattg aacaccactt catgtccacc tggattctcc aacctaaagg
    19081 cagacaaagc gtttgcgccg caggcgagtg caacatcgac atcaaacgcc gcaacgacaa
    19141 gctgtacagc accagcgtca gcgtgccttt agccgccatc caaaacggcg cgaaagccga
    19201 agcctccatc aacctctacg ccggcccgca gaccacatcc gtcatcgcaa acatcgccga
    19261 caacctgcaa ctggccaaag actacggcaa agtacactgg ttcgcctccc cgctcttctg
    19321 gctcctgaac caactgcaca acatcatcgg caactggggc tgggcgatta tcgttttaac
    19381 catcatcgtc aaagccgtac tgtatccatt gaccaacgcc tcttaccgct ctatggcgaa
    19441 aatgcgtgcc gccgcaccca aactgcaagc catcaaagag aaatacggcg acgaccgtat
    19501 ggcgcaacaa caggcgatga tgcagcttta cacagacgag aaaatcaacc cgctgggcgg
    19561 ctgcctgcct atgctgttgc aaatccccgt cttcatcgga ttgtattggg cattgttcgc
    19621 ctccgtagaa ttgcgccagg caccttggct gggttggatt accgacctca gccgcgccga
    19681 cccctactac atcctgccca tcattatggc ggcaacgatg ttcgcccaaa cttatctgaa
    19741 cccgccgccg accgacccga tgcaggcgaa aatgatgaaa atcatgccgt tggttttctc
    19801 cgtcatgttc ttcttcttcc ctgccggtct ggtattgtac tgggtagtca acaacctcct
    19861 gaccatcgcc cagcaatggc acatcaaccg cagcatcgaa aaacaacgcg cccaaggcga
    19921 agtcgtttcc taaatgccgc agcatgaaaa atgccgtctg aaacctgttc agacggcatt
    19981 tttattgccc accccctatc ggggcggaaa tcttcaaccc gcatacatca caaaaatcgt
    20041 cgggcgtttt ttcagattgg gcatttcttt tctttttcgc cactgcacga ttgtttgact
    20101 gatgatttcc tgtgtcggca aggtcaaatc cgtagccgtg cataaacgcg tttcaggatg
    20161 caggttttcc accgcatcgg caagcagcgc atcattgcgg taaggcgttt caataaaaat
    20221 ctgcgtctcg ccgcactggc gcgaacgctg ttccaaagcc cgaaaagcct gaatccgctc
    20281 gtttttttca gacggcagat agcctttaaa cgcaaaactc tgcccgttcg cacccgaagc
    20341 catcaaagcc agcagcaggc tggaaggccc gaccagcgga cgcacttcaa aaccgtgttt
    20401 atgcgccaat gccaccaaat tcgcacccgg atcggccaca gccgggcaac ccgcctcact
    20461 gacaatgccc atactgcgcc cttcttgcaa aggtttcagc aattccggca aagtcttcaa
    20521 atccgtatgt tcattcaacg tttgcagatt cagctcgcgg ataggcgtag tcacgcccaa
    20581 atgtttcaaa tgcgcacgcg ccgttttttc cgcctccacg acaaaatccg tcagcccgac
    20641 aatcgcctgt tgttcatgcg gcaacaggca cggcgtgtca ggcgtaccca aaggcgtagg
    20701 aatcaaatac aaaacaggag acatcattcc ctcactcatc ggttaaaaat gccgtctgag
    20761 cctttcagac ggcataaacg ggcagttaca gaacctccac gccctcattt ttcaagaaat
    20821 cgaccagacg gaaaaccggc aaaccgatta aagcattcgg atcggtactc tcaatccttt
    20881 caatcagcaa tgcacccaaa tcctcactct tcagcgcaca cgaacaataa accgcatcag
    20941 gctcgcgctc caaatagcgg aggatatgca actcgtccaa ctgcctcatc acgaccaccg
    21001 tcttatcgat atgccgccgc atcctgcccg taaccgtatt caacagcacg atcgcgctgt
    21061 aaaactcaat ctccctgccg ctcaagtgca tcagcatctt ttgcgcattg gcaaggttca
    21121 tcggcttgcc ccactgcctg ccgtcgcacc acgccacctg gtccgcaccg acaatcaacg
    21181 cctctgggaa acgcccggtc aacgaccgcg ccttaccctc ggcaaggcgc aatgccgtct
    21241 gaggggcgga ttcccccaac atcggcgttt cgtcaaaatc gggggacgcc gcctgaaagg
    21301 caatgccgag cctttccatc tgttcgcggc ggaaaaccga actcgtaccc aaaatcaaag
    21361 gcagttccaa acccatccca tcctccttac cgttgaaaac acgcccgaag gggcagtaaa
    21421 atccagccat gcgccgaaac acggataccc gccttcggcg taccgcaaca tttttcttaa
    21481 aaatattgac gttagaacat ctaaattata tcatatcccg tttatgtcag accctaattt
    21541 gattgacttg gaaatttttg ccgccgaagg gcagaacctg caaggcagtt ttctgctgga
    21601 agaattggat gaacgcgtca gttcgcacga ttatcccgcc gacaggcaga ccaaaatatc
    21661 gtttacactg accggcggtc gcgaccggct gcaacgcctg ttcctcgacc tgaacgtcaa
    21721 agccgatatg cccctgattt gccagagatg tatcaaaccc atgccgttca tgcttgatga
    21781 aagcagccgt atcgtcctgt tttccaacga agagtccttg gacgaatcca tgcttgccga
    21841 cgaagaactc gaaggcatac tgattgaaaa agaactcgac gtgcgcacat tggtagaaga
    21901 ccaaatcctg atgtccctgc ccttttcgcc gcgacacgaa gactgcggcg acaatgggac
    21961 actggaagaa gtcaatcggg acaaacccaa cccctttgct gttttggcag gtttgaaaag
    22021 caattgatta ggacacagtt tatttatcta ggagcttgaa atggccgttc aacaaaacaa
    22081 aaaatcccct tccaaacgcg gtatgcaccg ttcgcacgac gcgctgaccg cgcctgcact
    22141 gtctgtcgac agcacaaccg gcgaagtaca ccgcccgcac cacatctccc ccaacggtat
    22201 gtaccgcggc cgcaaagtgg tcaaagccaa aggcgaataa tccctattcg actgactgaa
    22261 aaagccagaa cattgccatg caattactgg ctttttttgc attggacgca ccatccgtcc
    22321 aaactttcgc catacgtcaa cacacagggg caaagcgttc cgtataatac cccgtgaaaa
    22381 tattccaaaa gccccaacca ccaaggaaat tccgatgaaa cagaaaatct ggtacaccta
    22441 cgatgacatc caccgcgtca tcaaagcatt ggcagaaaaa atccggaacg ccgacatcaa
    22501 atacgatgcc atgattgcca tcggcggcgg cggctttatt ccggcacgta tgctgcgctg
    22561 ttttctggaa attccgattt atgccgtaac caccgcctat tacgacagcg acaacgaagg
    22621 acaggttacc gaagaagtca aaaaagtcca atggctagac cccgtccccg aagccttacg
    22681 gggtaaaaac gtccttgttg tcgacgaagt ggacgacagc cgcgtaacca tggaattttg
    22741 cctgacagag ctgctcaaag aagacttcgg caccatcggc gtcgccgtac tgcacgaaaa
    22801 aatcaaagcc aaagcaggta aaatccccga aggcattccc tatttcagcg gcatcaccgt
    22861 agaagactgg tggatcaact atccgtggga cgcactcgac atcgacgaac acaaccgcct
    22921 tgccgaggcc ggccgaggct gaccctttca gacggcatat tttccgaacc gatgccgtct
    22981 gaagcccgca cgacccctgc cgcagaccga aaacctaccg gagaacccta tgattacact
    23041 tgccgtagat gccatgggcg gcgaccaagg acttgccgtt accgtacccg gcgcaaccgc
    23101 attcctccaa gcacaccccg atgtccgcct gattatgacc ggcgacgaaa cgcaactgcg
    23161 ccaagccctg accgcggcag gcgcaccgat ggaacgcatc gacatctgcc ataccaccca
    23221 agttgtcggc atggatgaat cccctcagtc cgccctaaag aacaaaaaag actcctccat
    23281 gcgcgttgcc atcaaccaag ttaaagaagg caaagcccaa gccgccgtat ccgcaggcaa
    23341 cacgggcgca ctcatggcaa ccgcacgttt cgtcctcaag accatacccg gtatcgaacg
    23401 ccccgccatc gccaaattcc tcccgtccga caccgaccat gttaccctcg ccctcgacct
    23461 tggcgcgaac gtcgactgca cgcccgaaca gctcgcccaa tttgccgtta tcggcagcga
    23521 actcgtccac gcactccatc ctcaaaaagg acagccgcgc gtcgggctgg tcaacgtcgg
    23581 cacggaagac atcaaaggta cggacaccgt caaacaaacc tacaaactgc tgcaaaacag
    23641 caaactcaac tttatcggca acatcgaaag caacggcatc ctgtacggcg aagcagatgt
    23701 cgtcgtcgcc gacggctttg tcggcaacgt catgctcaaa accatcgaag gcgcggtcaa
    23761 attcatgagc ggagccatcc gccgcgaatt ccaaagcaac ctgttcaaca aacttgccgc
    23821 cgttgccgcc ctacccgccc tcaaagggct gaaaaacaaa ctcgatccgc gcaaattcaa
    23881 cggggccatc ctgctcgggc tgcgcggcat cgtcatcaaa agccacggcg gcacagacga
    23941 aaccggtttc cgctacgcac tcgaagaagc ctaccacgaa gccaagtccg ccggcctttc
    24001 caaaatcgaa caaggcgttg ccgaacaact cgccgcactc gaaactgcca aagccgtcca
    24061 aaacgaaaat gccgacggtc tgtaacacac acgatgccgt ctgaacgccc ccgccccttt
    24121 cagacggcat ccgcccgcac caaacctgcg ggcgcggacg gcgatgcgcc tgtccggcac
    24181 ttcccaaata cttccctgta aaataaggag tatttgaaaa atgaagacat tagaaaaacg
    24241 gatgaaagct ctagacaaac agatgatgaa gttcggaaaa tcccttgaag gcaggcttga
    24301 tgcccgtctg attgaatccg cattggatta tattcattat tcggaacgtt ttttggcttt
    24361 tgaaatcctg tgtacttata tcgaagattt cgatgtccgg ctgacggaac aagaatcccg
    24421 ggaaatttct tttatcaaca aggaatttga gatagaaagc acgtccgatt aaccaataaa
    24481 gccaatgggt tgataaacat gaaaacatcg acggtcgttt ttggcggatt ttttatggca
    24541 gacaacggag agcgaatcca aatccccgtt ttggaaaatc ctgacattag ggaaatcaat
    24601 cacttttttt ccgtatcaaa ttttgagaaa aaaaccggcg tccttgtttt cagaatcatc
    24661 cccgagccgg aatttggcaa taccgaatta actgtctatt ttgaaaaagg atattatttg
    24721 ccaatgattc aaacaatttt agaaggcggg gatattgaag tcaaaaacct taaaacggaa
    24781 aattatagtg gaaatacaat ggaaatttgg ggagattcct accctattga gcatatctct
    24841 aaaaatattt ctacaattca aaatataatt tctgaattta tgatacaaaa acaaacaccg
    24901 gcaccaataa tttgatggaa aaccaacaat caaaatgccg tctgaagcat gtttggtttt
    24961 cagacggcat ttccttcgct taaaacagcg tatcggcaac cccgccctgc ctgtccacgg
    25021 caatctgcat ctgaaaacca tctgtatccc aaaccacacc cccatccctg tttccgtcat
    25081 ttgcaccctg cccgtattgg gcaatcatcg gtttttcgct tacaatagcc gaatctgaac
    25141 caactatcca aaaaggccgt tcccatgcag tatgccaaaa tttccggcac gggcagctat
    25201 cttcccgcca accgcgtcag caatgacgac cttgcccaaa aagtggatac ctcggacgag
    25261 tggattaccg cgcgtacggg catcaagttc cgccatattg cagccgaaaa tgagaaaacc
    25321 agcgatcttg ccgccgaagc agcccgccgc gcactggatg cagccggatt agacagcggc
    25381 gaaatcgatt tgattatcgt ggcgacggca acgccggata tgcagtttcc ttctaccgcc
    25441 accatcgtgc agcaaaaatt gggcatcacc aacggctgtc ccgcgtttga cgtacaggca
    25501 gtgtgcgcag gctttatgta cgcgctgacc actgcaaacg cctacatcaa aagcggcatg
    25561 gcaaaaaacg cgctggtcat cggcgcggaa accttcagtc gcattgtaga ctggaacgac
    25621 cgcacaacct gcgtattgtt cggcgacggc gcgggcgcgg tggttttgag cgcggcggac
    25681 aaaccgggca tcatccacag caaactcaaa gccgacggca attatctgaa actcttaaac
    25741 gtccccgggc aaatcgcctg cggcaaagtt tccggttcgc cgtacatttc gatggacggt
    25801 cccggcgtgt tcaagtttgc cgtcaaaatg ctgtccaaaa tcgccgatga cgttatcgaa
    25861 gaagccggtt ataccgccgc tcaaatcgac tggatcgtgc cgcatcaggc aaaccgccgc
    25921 attatcgaat cgaccgcgaa acatttaggt ttgagtatgg acaaagtcgt cctgaccgtc
    25981 caagaccacg gcaacacatc cgccgcatcg attccgctgg ctttggatac gggcatccgc
    26041 agcggacaaa tcaaacgcgg tcaaaacctg ctgctcgaag gcatcggcgg cggtttcgcg
    26101 tggggcgcgg tattgctgca atattgaata ccatgccgtc tgaagcaggt tttcagacgg
    26161 tatttcccat atcatgaagc ggcaggcttt cttcaaacca attgcgtgtg cggcatttct
    26221 gtctgccatt tcgctgcgcc tccccgtatt gggcgcgtgt tacgcaatat tgtccctcta
    26281 tgcgtttgca ctttacggca tcgacaaacg gcgtgccgtg cggggaaaac gccgcattcc
    26341 cgaacaccgc ctgctgctgc ctgccttgtt cggcggttgg gcgggcgcat acttgggcag
    26401 caggatattc aggcataaaa cggcgaaaaa gcgttttgtt gtgctgttcc gtctgactgt
    26461 ttcaggtaat gtcctggcga ccctcatcct gattgattat ttcgttccgc ccgaactttt
    26521 tgtcaaactc gggcaacatc tctgacaatc caaccaaaca aaggaatacc atgtcttttg
    26581 cattcttctt tcccggacaa ggttctcaaa gcctcggcat gatgaacggc tttacagggc
    26641 aggccgtcgt caaaaacaca tttgacgaag catccgccat attgggtcaa gacttgtggg
    26701 cgatgattaa cggtagcgat gccgaaatca tcggtcaaac cgtcaatacc caacccatca
    26761 tgctcgccgc cggcgttgcc gtttaccgcg cctatttgga agcgggcggc aaaacgcctg
    26821 ccgccgttgc cggacacagc ctcggcgaat ataccgcact cgttgccgcc ggcgcattgg
    26881 attttgccga cgcggtcaaa ctcgtgcgcc tgcgcgccga actgatgcag tctgccgtac
    26941 cgcaaggcgt gggcgcaatg gcggcgattc tcggcttgga agatgagcag gtaaaacaaa
    27001 tttgcgccga agccgcccaa ggcgaagtgg tcgaagccgt caacttcaac tcacccggac
    27061 aaatcgtgat tgcaggcaat gccgccgccg tcggacgcgc catggctgcc gccaaagaag
    27121 ccggtgccaa acgcgccctg ccgctgcccg tgtccgtacc ttcccattgc agcctgatga
    27181 aacccgccgc cgacaaactc gccgaagccc tcaaaaccgt cgaaatcaag cagccgcaaa
    27241 tccgcgtcat ccacaacgcc gacgttgccg cctacgatga tgccgacaaa atcaaagacg
    27301 cgctcgtccg ccagctttac agccccgtac gctggacaga aaccgtcaac gcccttgttt
    27361 cagacggcat tgccgaatcc gccgaatgcg gcccgggcaa agtgttggcg ggcttggcaa
    27421 aacgcatcaa caaggccgcc gcgtgcagcg cactgaccga tgccggacag gttgccgcct
    27481 ttatcgaagc gcactgactt cgttccgcaa aaagcagcct gccctcttca ggctgctttt
    27541 cacgtccgaa cggcaaacag ccgcatattt gcgctataat tgatgacgac caaaccaccg
    27601 acagcggctg ccgttgcagt tcccgcccta ccgatatgat agaaaaactg actttcggac
    27661 tgtttaaaaa agaagacgcg cgcagcttta tgcgcctgat ggcgtacgtc cgcccctaca
    27721 aaatccgcat cgttgccgcc ctgattgcca ttttcggcgt tgccgccacc gaaagctacc
    27781 ttgccgcctt catcgccccc ctgattaacc acggcttttc cgcacctgcc gcgccgcccg
    27841 agctgtctgc cgccgccggc atcatttcca ccctgcaaaa ctggcgcgaa cagtttacct
    27901 atatggtttg ggggacggaa aacaaaatct ggaccgtccc cctcttcctc atcatcctcg
    27961 tcgtcatccg tggcatctgc cgctttacca gcacctatct gatgacttgg gtctccgtga
    28021 tgaccatcag caaaatccgc aaagatatgt ttgccaaaat gctgaccctt tcctcccgct
    28081 accatcagga aacgccgtcc ggcaccgtac tgatgaatat gctcaacctg accgaacagt
    28141 cggtcagtaa cgccagcgac atcttcaccg tcctcacgcg cgacacgatg atcgttaccg
    28201 gcctgaccat cgtcctgctt tacctcaact ggcagctcag cctcatcgtt gtcctgatgt
    28261 tccccctgct ctccctgctc tcgcgctact accgcgaccg tctgaagcac gtcatttccg
    28321 actcgcaaaa aagcataggc acgatgaaca acgtgattgc cgaaacccat cagggacacc
    28381 gcgtcgtcaa gctgttcaac gggcaggcgc aggcggcaaa ccggtttgac gcggtcaacc
    28441 gcaccatcgt ccgcctcagc aaaaaaatca cgcaggcaac ggcggcacat tccccgttca
    28501 gcgaactgat tgcctcgatt gccctcgccg ttgtcatctt catcgccctg tggcaaagcc
    28561 aaaacggcta caccaccatc ggcgaattta tggcattcat cgtcgcgatg ctgcaaatgt
    28621 acgcacccat caaaagcctt gccaacatca gcatccctat gcagacgatg ttcctcgccg
    28681 ccgacggcgt atgtgcattt ctcgacaccc cgcccgaaca ggacaaaggc acgctcgcac
    28741 cgcagcgtgt cgaagggcgc atcagcttcc gcaacgtcga tgtcgaatac cgttcggacg
    28801 gcatcaaagc cctcgacggc ttcaacctcg acatcagaca aggcgaacgc gtcgccctgg
    28861 tcggacgttc cggcagcggc aaatccaccg tcgtcaacct gctgccccgc tttgtcgaac
    28921 cgtctgccgg caacatctgc atagacggta tcgacatcgc cgacatcaaa ctcgactgcc
    28981 tgcgcgccca atttgccctc gtctcccaag acgtattcct gtttgacgac accctgtttg
    29041 aaaacgtccg atatagccgt cccgacgcgg gcgaagccga agtcctatcc gccctccaag
    29101 ccgctaacct gcaaagcctg atcgacgcat caccgctcgg actgcaccag cccatcggat
    29161 cgaacggcag caacttatcc ggcggacagc ggcaacgcgt tgccattgcc cgcgccattt
    29221 tgaaagacgc gccgatatta ttattggacg aagccaccag cgcattggac aacgaatccg
    29281 aacgcctcgt ccaacaggcg ctcgaacgcc tgatggaaaa ccgcaccggc atcatcgtcg
    29341 cccaccgcct gaccaccatc gaaggggccg accgcatcat cgtaatggac gacggcaaaa
    29401 tcatcgaaca aggcacacac gaacaactga tgtcccaaaa cggctactac acgatgttac
    29461 gcaatatctc aaacaaagat gccgccgtcc ggacggcata aacaaaatgc cgtctgaaat
    29521 ggtacaatcg ccccgaccct ttcagacggc atcatatccg ccgacccatc cgattatctt
    29581 caatcactgt aaaacccatt atgacccaag acaaaatcct catccttgac ttcggttcgc
    29641 aagttaccca gctcatcgcc cgccgcgtgc gcgaagccca cgtttactgc gagctgcatt
    29701 ccttcgatat gcctttggac gaaatcaaag ccttcaaccc caaaggcatc atcctttccg
    29761 gcggccctaa ttccgtttac gaatccgact atcaagccga taccggtatc tttgacttgg
    29821 gcattccgat tttgggcatt tgctacggca tgcaatttat ggcgcaccac ttgggtggtg
    29881 aagtgcaacc cggcaaccag cgcgaattcg gttacgcgca agtcaaaacc atcgacagcg
    29941 agctgacacg cggcattcaa gacgatacgc caaacacgct cgacgtatgg atgagccacg
    30001 gcgataaagt ttccaaactg cctaccggtt tcaccgtcat cggcgacacg ccgtcctgcc
    30061 cgattgccat gatggaaaac gccgaaaaac aattctacgg catccaattc caccccgaag
    30121 taacccacac caaacaaggc cgcgccctgt tgaaccgctt tgtcttggat atttgcggcg
    30181 cgcaacctag ctggacgatg ccgaactaca tcgaagaagc cgttgccaaa atccgcgaac
    30241 aggtcggcag cgacgaagtg attttgggtt tgtccggcgg cgtggactct tccgtagccg
    30301 ccgcgctgat tcaccgcgcc atcggagacc aactgacctg cgtgttcgtc gatcacggcc
    30361 tcttgcgctt gaacgaaggc aaaatggtga tggatatgtt cgcccgcaat ttgggtgtga
    30421 aagtgattca cgtcgatgcc gaagggcagt ttatggagaa actcgccggc gttaccgatc
    30481 ccgagaaaaa acgcaaaatc atcggtgcgg aatttatcga agtatttgat gctgaagaaa
    30541 aaaaactcac caacgccaaa tggctggcgc aaggcacgat ttaccctgac gtaatcgaat
    30601 ccgcaggcgc gaaaaccaaa aaagcccacg ccatcaaatc ccaccacaat gtcggcggcc
    30661 tgcctgaaaa catgaagctc aagctgcttg agccgttgcg cgatttgttc aaagacgaag
    30721 tgcgcgaact gggcgtggct ttgggtctgc cgcgcgaaat ggtgtaccgc catcccttcc
    30781 ccggtcccgg tttgggcgtg cgcatcttgg gcgaagtgaa aaaagaatac gccgacttat
    30841 tgcgtcaagc agacgatatt tttattcaag aattacgcaa caccaccgac gaaaacggca
    30901 cgtcttggta cgacctgacc agccaagcct tcgccgtgtt cctgcctgtg aaatccgtcg
    30961 gcgtaatggg cgacggacgc acttacgact acgtcgtcgc actgcgcgca gtcatcacca
    31021 gcgactttat gaccgcacat tgggctgagt tgccttactc actgctcgga cgcgtgtcca
    31081 accgcatcat caacgaagtc aaaggcatca accgcgtggt gtacgatgtc agcggcaaac
    31141 cgcctgccac catcgagtgg gaataaccag caaacagggc tgcaccgtcc ggcgcagtct
    31201 ttcgattatc ggaaaaaagg aaaaaatatg agcacacaag atttaagcgg caaaatcgct
    31261 ttggtaacag gcgcctcgcg cggtatcggt gcagcaattg ccgacacact ggcggcagcc
    31321 ggtgccaaag tcatcggtac ggcgaccagt gagagcggtg cggcggcgat tagcgagcgg
    31381 ttggcgcaat ggggcggcga aggccgcgta ttaaattccg ccgaacccga aaccgtcgaa
    31441 aacctgattg ccgacatcga aaaaacgttc ggcaaactcg atattctggt caacaacgcc
    31501 ggcatcaccc gcgacaacct cctgatgcgt atgaaagaag aagagtggga cgacatcatg
    31561 caggtcaacc tcaaatccgt ctttcgcgcc tccaaagccg tcttgcgcgg catgatgaag
    31621 cagcgcgccg gccgtatcat caacatcaca tccgtcgtcg gcgtgatggg caatgccggt
    31681 caaaccaact atgccgcggc aaaagcaggc ttaatcggtt tctccaaatc catggcgcgc
    31741 gaagtcggca gccggggcat taccgtcaac tgcgtcgccc ccggctttat cgataccgac
    31801 atgacccgcg ccctgccgga agaaacccgc caaaccttta ccgcccaaac cgccttgggc
    31861 agattcggcg acgcgcaaga cattgccgat gcagtcttgt tcttggcttc cgatcaggca
    31921 aaatacatta ccggtcaaac gctgcacgtc aacggcggca tgctgatgcc ctgacgggca
    31981 gccgtaccga caatgccgtc tgaagccctt tcagacggca tttgcattct caggcaaaat
    32041 gaacacacgc cacaccccgc cctgcccctg cggatcaggc acaagc
//
LOCUS       DAWFAD010000005        31560 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWFAD010000005 DAWFAD010000000
VERSION     DAWFAD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31560
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.005"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..132
                     /locus_tag="WGO49_000162"
     CDS             <1..132
                     /locus_tag="WGO49_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0305329.1"
                     /translation="LAPYSPELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
     gene            complement(114..392)
                     /locus_tag="WGO49_000163"
     CDS             complement(114..392)
                     /locus_tag="WGO49_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305330.1"
                     /translation="MRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVGKQLTDSVGLE
                     FDPYYRHKTICKPREIVLDGDRTKMGRSKSNEYGFRVTATFYSQLKSK"
     gene            complement(481..686)
                     /locus_tag="WGO49_000164"
                     /pseudo
     CDS             complement(481..686)
                     /locus_tag="WGO49_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697603.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1038..2093
                     /gene="aroG"
                     /locus_tag="WGO49_000165"
     CDS             1038..2093
                     /gene="aroG"
                     /locus_tag="WGO49_000165"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ0305331.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            2161..2649
                     /locus_tag="WGO49_000166"
     CDS             2161..2649
                     /locus_tag="WGO49_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ0305332.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPAIDRTHWKEAERTERRVSSKGTSYAFVHYL
                     RY"
     gene            complement(2999..3475)
                     /locus_tag="WGO49_000167"
     CDS             complement(2999..3475)
                     /locus_tag="WGO49_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ0305333.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(3487..3915)
                     /locus_tag="WGO49_000168"
     CDS             complement(3487..3915)
                     /locus_tag="WGO49_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ0305334.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSDMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLILLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            4027..4272
                     /locus_tag="WGO49_000169"
     CDS             4027..4272
                     /locus_tag="WGO49_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305335.1"
                     /translation="MLKSIEFNSHIRNRLAAYLKGRGMDFQTAMREEKGNKEIASIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            5465..7603
                     /locus_tag="WGO49_000170"
                     /pseudo
     CDS             5465..7603
                     /locus_tag="WGO49_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            7845..9311
                     /locus_tag="WGO49_000171"
     CDS             7845..9311
                     /locus_tag="WGO49_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0305336.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(9512..10480)
                     /locus_tag="WGO49_000172"
     CDS             complement(9512..10480)
                     /locus_tag="WGO49_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305337.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPCAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAYLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            10557..11849
                     /locus_tag="WGO49_000173"
     CDS             10557..11849
                     /locus_tag="WGO49_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ0305338.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLIYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            complement(12352..13038)
                     /locus_tag="WGO49_000174"
     CDS             complement(12352..13038)
                     /locus_tag="WGO49_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ0305339.1"
                     /translation="MYAFTAAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDEFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
     gene            complement(13113..13586)
                     /gene="queF"
                     /locus_tag="WGO49_000175"
     CDS             complement(13113..13586)
                     /gene="queF"
                     /locus_tag="WGO49_000175"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ0305340.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFETLARKRLFEHDSQ"
     regulatory      complement(13590..13634)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            13863..15035
                     /gene="farA"
                     /locus_tag="WGO49_000176"
     CDS             13863..15035
                     /gene="farA"
                     /locus_tag="WGO49_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ0305341.1"
                     /translation="MATHGDEVKIQNRQEADSAKAKRKRCLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELIQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     gene            15059..16585
                     /gene="farB"
                     /locus_tag="WGO49_000177"
     CDS             15059..16585
                     /gene="farB"
                     /locus_tag="WGO49_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ0305342.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVVFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVVALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            16835..17068
                     /gene="rpmB"
                     /locus_tag="WGO49_000178"
     CDS             16835..17068
                     /gene="rpmB"
                     /locus_tag="WGO49_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ0305343.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            17100..17255
                     /gene="rpmG"
                     /locus_tag="WGO49_000179"
     CDS             17100..17255
                     /gene="rpmG"
                     /locus_tag="WGO49_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ0305344.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(17534..17842)
                     /locus_tag="WGO49_000180"
     CDS             complement(17534..17842)
                     /locus_tag="WGO49_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305345.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHDYGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKELLADLDKELC
                     "
     gene            complement(17826..18128)
                     /locus_tag="WGO49_000181"
     CDS             complement(17826..18128)
                     /locus_tag="WGO49_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ0305346.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(18202..20565)
                     /locus_tag="WGO49_000182"
     CDS             complement(18202..20565)
                     /locus_tag="WGO49_000182"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ0305347.1"
                     /translation="MQFSYSWLKTQADTELSADKLEHLLTMSGLEVEEAETAAPAFAG
                     VVVAEVKSVEKHPDADRLNVTRVDAGTGELVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVPSNGMLCSTNELGLPDDGVNGLHILPEDAPVGINIREYLDLDDTLFT
                     LKITPNRADCLSVKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISVLVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGALSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRSRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGYPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGM
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(20620..21645)
                     /locus_tag="WGO49_000183"
     CDS             complement(20620..21645)
                     /locus_tag="WGO49_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0305348.1"
                     /translation="MVSAIDSLKKNVNYNYVNSSNTHTIQIVHVEKPEGPIKIKRGDT
                     ETSISKQAIWRLADALSTGEPVNVDRVFGASYNFRSALEALLAHTPEIYWCKLQRIQP
                     GLNKSEVVEGHKHIIWLPDRPHEKGVMKEYETDVVISEIPSNNVVYDALSLPSTHSEI
                     DIDVKRRHVQIQIALSAIGFQLGFRTWIARNDQGISYGDKKIADLDGIVQKIESEKLI
                     SSFDGAVQAAMNIDCIWFRNGKLMPAVMEVEHSTGVRSGLARMKQLKDLLPPYANTRW
                     VIVAPDEDRDKVFKEANVPMFKNLDTQYFPYSAVEELYSLCIRRKLTNKAVNEEFLDC
                     FMEKCVM"
     gene            complement(21647..22741)
                     /locus_tag="WGO49_000184"
     CDS             complement(21647..22741)
                     /locus_tag="WGO49_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003683097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0305349.1"
                     /translation="MIFSFFSGAGFLDLGFELSGFDIAFVNEVHPPFLEAYKYSRSRM
                     DIPKPKYGYFKGSIDECLYAEKAKDLAGWVKKEKQNGIIVGFIGGPPCPDFSIAGKNK
                     GKDGENGKLSQSYVDLICKNQPDFFVFENVKGLYRTAKHREFFNALKRQLSDFGYVCT
                     EKLINAIEYGVPQDRERIILVGFLSQHVDALQKFDWDAHISFPDALEKDWPTTEEVGR
                     VVSQPANIYPELTVQYWFNRNGVDTHPNASKHFQPRAGLEKFQTISEGDDKKKSYKRL
                     HRWRYSPTAAYGNNEVHIHPYLPRRISAAEALAIQSLPKEFELPDNMTLSNMFKTIGN
                     GVPFLAAKGIAMTLKSYLENHYERTKTDGC"
     gene            complement(22812..23258)
                     /locus_tag="WGO49_000185"
     CDS             complement(22812..23258)
                     /locus_tag="WGO49_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HEZ0305350.1"
                     /translation="MDRLTPEQRKKCMQSNKSKGTKPELALAKAMWALGLRYRKNSGS
                     IFGKPDFSFKKYKVAVFVDGEFWHGKDWEQRKAEIKGNREFWIAKIERNIRRDMEVTG
                     RLKVEGWAVLRFWSNDVVKNTTCCAEKVRQAVRDKQGLSKFQTREK"
     gene            complement(23582..24574)
                     /gene="pheS"
                     /locus_tag="WGO49_000186"
     CDS             complement(23582..24574)
                     /gene="pheS"
                     /locus_tag="WGO49_000186"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0305351.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSGEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFAK"
     gene            complement(24920..25279)
                     /gene="rplT"
                     /locus_tag="WGO49_000187"
     CDS             complement(24920..25279)
                     /gene="rplT"
                     /locus_tag="WGO49_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ0305352.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKSALAA"
     gene            complement(25292..25489)
                     /gene="rpmI"
                     /locus_tag="WGO49_000188"
     CDS             complement(25292..25489)
                     /gene="rpmI"
                     /locus_tag="WGO49_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ0305353.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(25636..26157)
                     /gene="infC"
                     /locus_tag="WGO49_000189"
     CDS             complement(25636..26157)
                     /gene="infC"
                     /locus_tag="WGO49_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ0305354.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(26175..28088)
                     /gene="thrS"
                     /locus_tag="WGO49_000190"
     CDS             complement(26175..28088)
                     /gene="thrS"
                     /locus_tag="WGO49_000190"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ0305355.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDACDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVAVKLSLRPEQRAGSDEVWDKAEQGLRDALTACGVE
                     WEELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(28141..28217)
                     /locus_tag="WGO49_000191"
     tRNA            complement(28141..28217)
                     /locus_tag="WGO49_000191"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28181..28183),aa:Val,seq:gac)
     gene            28396..29511
                     /gene="tgt"
                     /locus_tag="WGO49_000192"
     CDS             28396..29511
                     /gene="tgt"
                     /locus_tag="WGO49_000192"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ0305356.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQAKKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRTGEILGAQLNTIH
                     NLHFYQVIMAEMREAVEQGKFADWQARFHENRARGTD"
     gene            29521..30603
                     /locus_tag="WGO49_000193"
     CDS             29521..30603
                     /locus_tag="WGO49_000193"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ0305357.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAKRMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHHDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            30795..31196
                     /locus_tag="WGO49_000194"
     CDS             30795..31196
                     /locus_tag="WGO49_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0305358.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGESRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(31259..31549)
                     /locus_tag="WGO49_000195"
     CDS             complement(31259..31549)
                     /locus_tag="WGO49_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305359.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         7763 a         8060 c         8038 g         7699 t
ORIGIN      
        1 cttgcacctt attcacctga gctcaacccg attgagaaag tgtgggcgaa tattaagcgg
       61 tatctgcgaa ccgttttgtc tgattacgcc cgatttgacg atgcactact gtcctacttt
      121 gattttaatt gactatagaa cgttgcggtt acgcggaagc cgtactcgtt ggatttggag
      181 cggcccattt tggttctgtc gccgtccaag acaatctcac ggggtttgca gattgttttg
      241 tgacggtagt atggatcaaa ctcgagaccg acgctgtcgg tcaactgttt gcctacattc
      301 agaccgatac cgacactcca acctttggcg cttttgctga catcgcggga agcacacatc
      361 tgggtcgtca tcactttggt tttgccgcgc aaatctgcat atgcatccgc ccaaggggtt
      421 agggatcatc cgtcccccaa atcttggcgg atttcgccat ggactttcaa agcaaggttt
      481 tcatgcttgg taacggtgtt tttccttatc gccgatgatg gctttgcctt tgccgttaga
      541 ctcgggaata tcggctaccg taacggcgga cacggctgca agtgagagtg caagcagggt
      601 tttttcatgt ttttcttcct ataatgagga taaataaatg gaaaaagtgt gggaaatacc
      661 cgcattccca ttaaatcttt tttcaagcaa tgagttcttt ttgttttcaa cattttcctt
      721 gtgtatgaaa ttttgccgga tgtgcaggcg gaatcggcag cggggtgttc tgtaccggat
      781 tgtcgtggaa atgggaaaac ggatgttccg tgcggttttg tccgaatgaa tggcgggtat
      841 tgtttttatc aatctgtttc tttttctttg gaataaaatt cctaaaatat taaaaatatg
      901 aaatttaaaa tctatgaaaa aagatatatc agttattttg aaataaaata gctttgtagt
      961 aatatgttgc acttgtttgt gcaaggtaaa cgatgtaacc taagccgcgt ataaaaaccc
     1021 atcaggaaag atgcaagatg acacaccatt accccacaga cgatattaag attaaagaag
     1081 ttaaagagtt gttgccgccg attgcccatc tttacgagct gccgatttcc aaagaggctt
     1141 cgggcttggt tcaccgcacc cgtcaggaaa tttccgattt ggttcacggc agggacaagc
     1201 ggctgttggt tattatcggg ccgtgttcga ttcacgatcc gaaagcggcg ttggaatatg
     1261 cggagcgttt gttgaaactc cgcaagcagt atgaaaacga gcttttgatt gtgatgcgcg
     1321 tttatttcga gaagccgagg acgacggtgg gttggaaagg tttgattaac gacccgcatt
     1381 tggacggtac gtttgacatc aatttcggtt tgcgtcaggc gcgcagcctg ttgttgtcgc
     1441 tgaacaatat gggtatgcct gcctctaccg agtttttgga tatgattacg ccgcaatatt
     1501 atgcggactt gatttcttgg ggggcaatcg gtgcgcggac gaccgaaagc caagttcacc
     1561 gcgaattggc aagcgggctg tcctgccccg tcggctttaa aaacggtacg gacggcaatt
     1621 tgaagattgc catcgacgca atcggtgcgg cgagccattc gcatcatttc ttgtctgtaa
     1681 ccaaggccgg acattccgcc attgtccata ccggcggcaa tcccgactgt catgtcattt
     1741 tgcgcggcgg aaaagagccg aattatgatg cgggacacgt cagcgaggcg gcggaacaac
     1801 tgcgtgcggc aggggtaacc gataagctga tgatagattg cagccacgcc aacagccgca
     1861 aggattacac ccggcagatg gaagtggcac aagatattgc tgcccaattg gaacaggacg
     1921 ggagcaatat tatgggcgtg atggtggaaa gccatttggt cgaaggcaga caggataagc
     1981 cggaagtgta cggcaaaagc attaccgatg cgtgtatcgg ttggggcgcg actgaagaac
     2041 tgttggcatt gttggcaggt gcaaacaaaa aacgtatggc gcgcgccggt tgagattttt
     2101 gacacggaat gtcataaaat gccgtctgaa gcgttcagac ggcatttttg tggaggaaat
     2161 atgctcaaaa taaccctaat tgcggcgtgt gcggaaaacc tgtgcatcgg ggcgggcaat
     2221 gctatgcctt ggcacatccc cgaagatttc gcatttttca aagcctatac cttgggcaaa
     2281 cccgtcatta tggggcggaa aacgtgggaa tccctgcccg tcaaacccct gcccggacgg
     2341 aggaacatcg tcatcagccg gcaggcggat tattgcgcgg caggcgcgga aacggcggca
     2401 agtttggagg cggcattggc attgtgcgca ggcgcggaag aagccgtcat tatgggcggc
     2461 gcgcagatat acggacaagc gatgccattg gcgaccgatt tgcggataac cgaagtggat
     2521 ttgtctgtgg aaggagatgc atttttcccc gcaatagacc ggacgcattg gaaagaagca
     2581 gagcggacgg aacgccgtgt cagcagcaaa ggcacgagct atgcttttgt gcattatttg
     2641 agatattgaa atataaactc tctataaaat cccccgcaaa tgatgggctg aaatagaaaa
     2701 tattgttatt cccccgaaga tgggaatccg ggattttaaa gttagggtaa tttatccgaa
     2761 ataacaacaa tcttccatcg tcattcccgc aaaagcggga atccggaaac gaaaagctaa
     2821 agcaatttat cggaaaaaac cgaagtttaa agaaccggat tcccgcctgc gcgggaatga
     2881 cgagatttta ggttatgggg atttattggg aataatggaa caaagaaagc agaaataagg
     2941 atatagaggc tgtctttgga tttgcgatgg ttgtcggaga atgccgtctg aagccgtttc
     3001 agacggcatt tttccagctt gagaacggat gcctgctcaa ataagcattg gtaaacatac
     3061 cgtcggcagt gatttcccgt cccagccagt ccggacggtc aaaatcggca ttctcgtcgg
     3121 gcaactcgat ttccgcgacg accaaaggcg cattatcgcc aagaaaaaca tcgatttcaa
     3181 acaggctgcc gccccatctg accggataac gccatttttc cattttaaac gggcacatcg
     3241 tttccatcat cttttccgca tcggcaagcg ggatttcgta ttcaaactca ctgcggctga
     3301 tttccgaaat atagcctttc agcgtcagcc acgcccgttg tccggcaatg cggacacgga
     3361 cggtgcgttc tttttcaaca gacagataac cctgcctcaa cagcagcggt tcgtcggcgt
     3421 attgccgcca gttgtcgttt ccaatcaaaa aacggcgttc gatttctatc ggcataagat
     3481 gctccgtcaa aacggtttga acacgaccag atacagcgcg gcaaccatca gcagcacggg
     3541 gatttcgttg aacacgcggt accagcggtg tgaaaaagca ttgctgtaat cctgaaaacg
     3601 gcgcagcagc acgccgcaat acaactggta agccaagagt atcaagccca aacacagttt
     3661 gacgtgtacc cagccgctgc cccaccagcc ggcggcaaac ggtatcgccg cgccgaacac
     3721 gaccgcgccg aagcccaacg gcgacataaa acggtacagc cgcaccgcca tatccgacag
     3781 gcgcacatac tcgggattgc cgcgcggtgc atcaatcatc gccatattga cgaaaatcct
     3841 cggcaggtaa aacagccctg caaaccacga aatgacaaaa aacaagtgaa acagcttgaa
     3901 ccaagaaaac atcatcgccc acaccctgcc gaaaagcggt attgtacagg caaaccgctt
     3961 gggaaacgtg ataaaatcag gcggataaac aaatcgaata aatccttacc gcaaaacgga
     4021 ggcaaaatgc tcaaatccat cgaattcaat tcccacatcc gcaaccgcct tgcagcatat
     4081 ctgaaaggca ggggtatgga ttttcagacg gcgatgcggg aggaaaaagg caacaaagaa
     4141 atcgcctcca tcgtccacag cggtctgccc actctggtcc gcaaactgta ttccgaacaa
     4201 aaaatgcaga agtttttttg ggaaaagcgg gatttgattg ccgactacat cagccgccgg
     4261 atgcagggat aggcggctga aatctgtttt caggcaagtg aaaagacaat atggcagatt
     4321 gaaattatgc ttatcgtcat tcccgcccgc gcgggaatcc gacttgtttg gtttcggtta
     4381 tttttcgttt cgtaactttt gagccgtcat tcccgcgcag gcggtaatcc ggcttgttcg
     4441 gtttcggttc tttttctcgt ttcgggtgat ttctaaaccg tcattcccgc gcaggcggga
     4501 atctaggtct ttaaacttcg gttttttccg ataaattttt gccgcattaa aattctagat
     4561 tcccactttc gcgggaatga cggcggaggg tttttagttt tcccgaaaat gcacatcatc
     4621 caaaatcccg ttattcccac aaaacagaaa atcaaaaaca gtaacctgaa atcccgtcct
     4681 tccctcccgt gcgggaatcc gacttatctg gtttcggttc tttttctcgt ttcaggtgat
     4741 ttctaaaccg tcattcccgc gcaggcggga atccagacct tcaaaccccg gacatccccg
     4801 ataaattctt gcggcattga aattctagat tcccgctttc acgggaatga cggcggaggg
     4861 tttttagttt tcccgataaa tgcacatcat ccaaaatccc gttattccca caaaacagaa
     4921 aatcaaaaac aacaatctga aattccgtcc ttcccgcgca ggcggtaatc tgaacacgtc
     4981 cgtagtgaaa cctatatccc gtcattcgca cgaaagtggg aatccaggat gcagggaaaa
     5041 ccgttttatc cgataagttt ccgcaccgaa aggtctagat tcccgctttc gcgggaatga
     5101 cggcggaggg tttttagttt tctcgataaa tgcacatcat ccaaaatccc gttatttcca
     5161 caaaacagaa aatcaaaaac agtaacctga aatcccgtca ttcccgcgca ggcgggaatc
     5221 cggcttgttc ggtttcggtt ctttttcttg tttcgggtga tttctaaacc gtcattcccg
     5281 cgcaggcggg aatccagacc tttaaacccc gaccatcctt gataaattct tgcggcatta
     5341 aaattctaga ttcccgcttt cgcgggaatg acggcggagg gttttttgct tttcctgatt
     5401 tttcattgcg atgtagtata atgtagtata taatcattat aattttaaca cttgacaaag
     5461 gaaaatttct catgacactg aaagcaagca agcaagcaag caagcaagca agcaagcaag
     5521 caagcaagca agcaagcaag caagcaagca agcaagcggt cgggttaatc tattaacatt
     5581 atctgtttta tcgctgtttt gcacgccata tgtttgaggt tcggatgcgt acgatcccgt
     5641 caaagaagcc gagattaaaa acaaatttat tttagaagcg gcggaagaca gaaattccca
     5701 cgtttggcgc ggcccgtgca gcatatcttt tgattgcttc ggtatgttca gagctcagct
     5761 tggttcaaat actcgttcta ccaaaatcgg cgacgatgcc gatttttcat tttcagacaa
     5821 gccgaaaccc ggcacttccc attatttttc cagcggtaaa accgatcaaa attcatccga
     5881 atatgggtat gacgaaatca atatccaagg taaaaactac aatagcggca tactcgccgt
     5941 cgataatatg cccgttgtta agaaatatat tacagatact tacggggata atttaaagga
     6001 tgcggttaag aagcaattac aggatttata caaaacaaga cccgaagctt gggaagaaaa
     6061 taaaaaacgg actgaggagg cgtatataga acagcttgga ccaaaattta gtatactcaa
     6121 acagaaaaac cccgatttaa ttaataaatt ggtagaagat tccgtactca ctcctcatag
     6181 taatacatca cagactagtc tcaacaacat cttcaataaa aaattacacg tcaaaatcga
     6241 aaacaaatcc cacgtcgccg gacaggtgtt ggaactgacc aagatgacgc tgaaagattc
     6301 cctttgggaa ccgcgccgcc attccgacat ccatacgctg gaaacttccg ataatgcccg
     6361 catccgccta aacacgaaag atgaaaaact gaccgtccat aaagcgtatc agggcggcgc
     6421 ggatttcctg ttcggctacg acgtgcggga gtcggacgaa cccgccctga cctttgaaca
     6481 aaacgtcagc ggaaaatcca atgtggtttt ggaacgccgg ccggaaaatc tgaaaacgct
     6541 cgacgggcgc aaactgattg cggcggaaaa ggcagaccct aattcgtttg cgtttaaaca
     6601 aaattaccgg cagggactgt acgaattatt gctcaagcaa tgcgaaggcg gattttgctt
     6661 gggcgtgcag cgtttggcta tccctgaggc ggaagcggtt ttatatgccc aacaggctta
     6721 tgcggcaaat actttgtttg ggttgcgtgc cgccgacagg ggcggcgacg tgtatgccgc
     6781 cgatccgtcc cgtcaaaaat tgtggctgcg cttcatcggc ggtcggtcgc atcaaaatat
     6841 acggggcggc gcggctgcgg acgggcggcg caaaggcgtg caaatcggcg gcgaggtgtt
     6901 tgtacggcaa aatgaaggca gccggctggc aatcggcgtg atgggcggca gggccggcca
     6961 gcacgcatca gtcaacggca aaggcggtgc ggcaggcagt tatttgcatg gttatggcgg
     7021 gggtgtttat gctgcgtggc atcagttgcg cgataaacaa acgggtgcgt atttggacgg
     7081 ctggttgcaa taccaacgtt tcaaacaccg catcaatgat gaaaaccgtg tggaacgcta
     7141 caaaaccaaa ggttggacgg cttctgtcga aggcggctac aacgcgcttg tggcggaagg
     7201 cgttgtcgga aaaggcaata atgtgcggtt ttacctgcaa ccgcaggctc agtttaccta
     7261 cttgggcgta aacggcggct ttaccgacag cgaggggacg gcggtcggac tgctcggcag
     7321 cggtcagtgg caaagccgcg ccggtattcg ggcaaaaacc cgttttgctt tgcgtaacgg
     7381 tgtcaatctt cagcctttcg ccgcttttaa tgttttgcac aggtcaaaat ctttcggcgt
     7441 ggaaatggac ggcgaaaaac agacgctggc aggcaggacg gcgctcgaag ggcggttcgg
     7501 cattgaagcc ggttggaaag gccatatgtc cgcacgcatc ggatacggca aaaggacgga
     7561 cggcgacaaa gaagccgcat tgtcgctcaa atggctgttt tgatgcgtcg ggaaatgttt
     7621 tgacgcacag gcggcacacc ggcacggccc cgcgcgccgc cccgcaaacc aatccgaacc
     7681 ctgccgcccc gaagggcggg gcatcatgat gaaaccggcg gaaaaccgcc ggttttttgc
     7741 cgccgtttga aacccgattc tggcttcaga cggcattgtc gcggcatcgg gcggcagggt
     7801 ttggaacagc ggcataaaaa actgatacaa tccgccgatt gataatggtt attttttatt
     7861 tttgtgggaa gacatttatg cctgcacgaa acagatggat gctgctgctg cctttattgg
     7921 caagcgcggc atatgccgaa gaaacaccgc gcgaaccgga tttgagaagc cgtcccgagt
     7981 tcaggcttca tgaagcggag gtcaaaccaa tcgacaggga gaaggtaccg gggcaggtgc
     8041 gggaaaaagg aaaagttttg cagattgacg gagaaaccct gctgaaaaat cccgaattgt
     8101 tgtcccgcgc gatgtattcc gcagtggtct caaacaatat tgccggtatc cgcgttattt
     8161 tgccgattta cctacaacag gcgcagcagg ataagatgtt ggcactttat gcacaaggga
     8221 ttttggcgca ggcagacggt agggtgaagg aggcgatttc ccattaccgg gaattgattg
     8281 ccgcccaacc cgacgcgccc gccgtccgta tgcgtttggc ggcggcattg tttgaaaaca
     8341 ggcagaacga ggcggcggca gaccagttcg accgcctgaa ggcggaaaac ctgccgccgc
     8401 agctgatgga gcaggtcgag ctgtaccgca aggcattgcg cgaacgcgat gcgtggaagg
     8461 taaatggcgg cttcagcgtc acccgcgaac acaatatcaa ccaagccccg aaacggcagc
     8521 agtacggcaa atggactttc ccgaaacagg tggacggcac ggcggtcaat taccggctcg
     8581 gcgcggagaa aaaatggtcg ctgaaaaacg gctggtacac gacggctggc ggcgacgtgt
     8641 ccggcagggt ttatccgggg aataagaaat tcaacgatat gacggcaggc gtttccggcg
     8701 gcatcggttt tgccgaccgg cgcaaagatg ccgggctggc agtgttccac gaacgccgca
     8761 cctacggcaa cgacgcttat tcttacacca acggcgcacg cctttatttc aaccgttggc
     8821 aaaccccgaa atggcaaacg ttgtcttcgg cggagtgggg gcgtttgaag aatacgcgcc
     8881 gggcgcgttc cgacaatacc catttgcaaa tttccaattc gctggtgttt taccggaatg
     8941 cgcgccaata ttggatgggc ggtttggatt tttaccgcga gcgcaacccc gccgaccggg
     9001 gcgacaattt caaccgttac ggcctgcgct ttgcctgggg gcaggaatgg ggcggcagcg
     9061 gcctgtcttc gctgttgcgc ctcggcgcgg cgaaacggca ttatgaaaaa cccggctttt
     9121 tcagcggttt taaaggggaa aggcgcaggg ataaagaatt gaacacatcc ttgagccttt
     9181 ggcaccgggc attgcatttc aaaggcatca cgccgcgcct gacgttgtcg caccgcgaaa
     9241 cgcggagtaa cgatgtgttc aacgaatacg agaaaaatcg ggcgtttgtc gagtttaata
     9301 aaacgttctg attgctgttc cttttcggag gaaaccctgc cggcggcggt atcacggcgg
     9361 gcatcggcgg ctttcgggcg gtgctttgcg tgccgccgcg tgtgcggaaa cgcattccgg
     9421 tttttccggc ataacggcga tgcgaggtaa aatgccgtct gaaacccgat tcgggcttca
     9481 gacggcattg tcgcggttgc ggcgggcggg ttcaccagat tccgtcaaag gttttcgcgc
     9541 cgcgccaaaa tttccacctg tcggcgggtt tgaaggtcag cgtaccgccg tgttgtccgt
     9601 ccgtggcgat gtccagccgt ttgattttgc cgatgcggac ggcttcgtaa agcggtgcga
     9661 accagcgttc ttcccactgc tgcaatattg ccgcataccg ctccctgtcc cctgtcaggg
     9721 cggtcaggcg caaatcgtcc ataaacagga tatggtgcgt gtcgggtagg tatgccgccg
     9781 tttcttcata ggcgcggaag ttgtcgggca atgcgcggcg gtcggggtgg aaacggctcc
     9841 aaaccgtatc ggcgaaaagc gtgccgcctt gactgcttcc gtgcagactg tcccaaagcc
     9901 ataagccgtt caactcgggc agcccgcgtt tcttgcggtt atggttgacg gggtgcgccg
     9961 ccagccacat ttggatttcg gtttggacgc gcagccattc caacgcgtct tccccgtccg
    10021 gctgatcgtc cgcgcccaac aatccgccca agtccaaaac gggcttcgcg ccccagcggt
    10081 acgcgcaagg aagggaaacc agccataatt cgggcaggac gggaacgaaa cgccatggaa
    10141 tgtcgccgta aaacgccgac aggtcgcggc agagccgttc cgcttcatcc gtaccgacgt
    10201 tcagatattc cgccgttagc acatttgcct gatgcatccc catcttttgc cagacgggcg
    10261 tggcgagcgc gacggtttca gacggcatat tcaggctttg cgccgcgcgt tccgccagtc
    10321 tgccgcacca caaataacgc gcgtaaaatg ccgaagccgt gcagctttgg cggtgcagcg
    10381 agccgtattg caggattttg ttgaaggcgg gcaggcatag aggtattcgg atttcgtctt
    10441 catccaaatt gagcgagggg agggcgaggg tgagtttcat cgtttgatgt ctgacaaatg
    10501 cggatcgggc ggatattata gaggattcgg cgcaaacgcc gtcaaaaagg aacaatatgg
    10561 ctgtcttccc actttcggca aaacatcgga aatacgcgct gcgcgcgctt gccgtttcga
    10621 ttattttggt gtcggcggca tacattgctt tgacagagag gacggaacgc gtcagaccgc
    10681 agcgcgtgga acaaaatctg ccgccgctgt cttggggcgg cagcggcgtt cagacggcat
    10741 attgggtgca ggaggcggtg cagccgggcg actcgctggc ggacgtgctg gcgcgttcgg
    10801 gtatggcgcg ggacgagatt gcccgaatca cggaaaaata tggcggcgaa gccgatttgc
    10861 ggcatttgcg tgccgaccag tcggttcatg ttttggtcgg cggcgacggc ggcgcgcgcg
    10921 aagtacagtt ttttaccgac gaagacggcg agcgcaatct ggtcgctttg gaaaagaaag
    10981 gcggcatatg gcggcggtcg gcttctgagg cggatatgaa ggttttgccg acgctgcgtt
    11041 cggtcgtggt caaaacgtcg gcgcgcggtt cgctggcgcg ggcggaagtg cccgtcgaaa
    11101 tccgcgaatc cttaagcggg attttcgccg gccgcttcag ccttgacggt ttgaaggaag
    11161 gcgatgccgt gcgcctgatt tacgacagcc tgtatttcca cgggcagcag gtggcggcgg
    11221 gcgatatttt ggcggctgaa gtcgttaagg gcggcacaag gcatcaggcg ttctattacc
    11281 gttcggacaa ggaaggcgga gggggcggca attattatga tgaagacggc agggtgttgc
    11341 aggaaaaagg cggcttcaac atcgagccgc tggtctatac gcgcatttct tcgccgttcg
    11401 gctaccgtat gcaccccatc ctgcacactt ggcggctgca cacgggcatc gattatgccg
    11461 caccgcaggg aacgccggtc agggcttccg ccgacggcgt gattaccttt aaaggccgga
    11521 agggcggata cggcaacgcg gtgatgatac gccacgccaa cggtgtggaa acgctgtacg
    11581 cgcacttgag cgcgttttcg caggcacaag gcaatgtgcg cggcggcgag gtcatcggtt
    11641 ttgtcggttc gaccgggcgt tcgacggggc cgcacctgca ttacgaggcg cgcatcaatg
    11701 ggcagcccgt taatcctgtt tcggtcgcat tgccgacacc cgaattgacg caggcggaca
    11761 aggcggcgtt tgccgcgcag aaacagaaag cggacgcgct gcttgcgcgc ttgcgcggca
    11821 taccggttac cgtgtcgcaa tcggattgaa gtttgaaccg gcgacgaaaa caatgccgtc
    11881 tgaaaacctg caaacaggtt ttcagacggc atttatagtg gattaacaaa aaccagtacg
    11941 tcgttgcctc gccttagctc aaagagaacg attctctaag atgctgaagc accaagtgaa
    12001 tcggtttcgt actatttgta ctgtctgcgg cttcgtcgct ttgtcctgat ttttgttaat
    12061 ccaccataca gtcgattaaa ataaaactaa gttaaggaag cactgccgtc attcccgtac
    12121 cgtcgggaat ccggacacca cggcacggaa acccatccgc cgtcattccc gcgaaagcgg
    12181 gaatctagaa atacaatgcg gtaggagttt atcggaaatg actgaaaccc aacgtaccgg
    12241 attcccgctt tcgcgggaat gacgaagtgg gcgggaatcc ggatttatcc gttccgacag
    12301 tgtttgcaaa taaaagaaaa cccaaccgtc ccgattcccg gcagggctgt tttacggatt
    12361 ttgcagcgag ggcgcggggc ggtcttgcgc ctgtttggtt tgcagggttg tcagtttttt
    12421 cgtcagcaga ttcagaatca cgccgtaggc gggcaggaaa aacagaccgc agacggtaag
    12481 tttgaacagg taatcgacaa aagcgatgcc ctgccagttt gccgccataa attcatcgct
    12541 gctggcgtaa aaggcaacgg caaaaaacac taacgtgtcc agtgcattgc cgatgacggt
    12601 tgatgcggtc ggtgcaatcc accacgcttt cagacgacgt aatttgttga acacaaaaat
    12661 atcaaggatt tgtccgagcg cgtaggcggc aaagctggcc aaggcgatgc gtccgacaaa
    12721 ggtgttgaat tcggatagtg cgcccaagcc tgtccaactg ccgttgtgga acaaaacgga
    12781 aaagacgtag gaaagcaaaa gggcggggaa catcacccaa aagataatcc gccgtgccaa
    12841 gtgcgaaccg aaaatgcgga cggtcaggtc ggtggcgagg aagatgaagg gaaaggaaaa
    12901 cgcaccccaa gtggtgtgga tgccgaaaat ttggaagggg aactgcacca gatagttgct
    12961 ggcggcgatg atgaggatat ggaaaagcac cagccagaag agtgccttct gttgctgtgc
    13021 ggcagtaaat gcgtacataa aaatctttcg gaaaggcgtt cagacggcat atcgtatcga
    13081 aggaatgccg tctgaaatat gggaaggatg gtttattggc tgtcgtgttc gaacaagcgt
    13141 ttacgcgcca atgtttcgaa ctcggtgcct gctttgccgt aattggcgaa aggatgaatg
    13201 gcgatgccgc cgcgcggtgt gaactcgccg aatacttcga tgtatttcgg atccatcagg
    13261 gcaatgaggt ctttcatgat gatgttgacg cagtcttcat gaaaatcgcc gtggttgcgg
    13321 aagctgaaga ggtagagttt cagggatttg ctttccacca ttttgatgtg cggaatgtag
    13381 cggatgtaga tggtggcgaa gtcgggctgc ccggtcatgg ggcagaggct ggtgaactct
    13441 gggcagacga atttgacgaa atagtcgttg tcgggatgtt tgttgtcgaa tgcttcgaga
    13501 atttcaggcg cgtagccggt cggatatcgg gttttttgat tacccaaaag cgagatgcct
    13561 tgcagctctt cggtattgcg ggacataggg tttccttagt tttttagtgt gggaggtttt
    13621 cgaaccacgg ggcgacgatt gtaatataag cggcggtatc tgtgtagcgt ccccgcccgg
    13681 tacggcgcgc ttatccgatt atttttgaac ataaattaat tttgcggaac gcgggtgtgg
    13741 cggtttattt gaatattcat tctaaatcat taacttgatt gggttattgc cagggtatag
    13801 aatccgagct gcctttgatc ggctttattt atatcgtatt aaaaatattc ggaattggaa
    13861 taatggcaac acacggagac gaagtgaaaa ttcaaaacag acaagaagcc gatagcgcaa
    13921 aagccaaacg caaacgctgc ctgacggcat tgacgctgct gttcgcgctt gccgccgcag
    13981 ccgccgggtc ggcgtttttt ttatggtggc agcacgaaga ggaaacggaa gacgcttatg
    14041 ttgccggacg cgtggttcag gttacgccgc aaaagggcgg tacggtgcgg aaggttttgc
    14101 acgacgatac ggatgccgtg aaaaaaggcg acgtgctggc ggtattggac gacgataatg
    14161 atgtgctggc ttacgagcgg gcaaaaaacg agctgattca ggcggtgcgg caaaaccgcc
    14221 ggcaaaatgc cgccacttcg caggcggggg cgcaggttgc cttgcgccgg gcggatttgg
    14281 cacgcgcgca ggatgatttg cgccgccggt ctgctttggc ggaatcgggc gcggtgtccg
    14341 ccgaagagct ggcacatgcc cgtgcggcag tgtctcaggc tcaggcggca gttaaggcgg
    14401 ctttggcgga agaatcttcg gcgcgtgcgg ctttgggcgg tcaggtttct ttgcgcgaac
    14461 agccggcggt tcagacggca atcggcaggt tgaaagatgc gtggttgaac cttcagcgga
    14521 cgcaaatccg cgcgccggcg gacggtcagg tggcgaagcg ttcggtgcag gtcgggcagc
    14581 aggtggcggc aggcgcgccg ctgatggcgg tggtgccgct gtcggatgtg tgggtggatg
    14641 ctaattttaa agagacgcag ttgcggcata tgaaaatcgg acagcctgcc gagctggtgt
    14701 ccgatttgta cggcaaacaa attgtttatc gcggcagggt ggcaggtttt tcggcaggta
    14761 cgggcagcgc gttttcgctg attccggcgc aaaacgcaac gggcaactgg attaaagtgg
    14821 tgcagcgcgt ccccgtccgt atcgtgctga accgcgaaga tgtggacagg catccgttgc
    14881 gtatcggttt gtcgatgacg gttaaagtgg atacttccgc cgcaggcgcg cctgtttcaa
    14941 aaacgccggg tgcggcattg ccggaaatgg aaagtaccga ctggtcggaa gtcgatcgga
    15001 cggtcgatga aatcctcggg caatccgcgc cctgatgccg tctgaaacgg aggacacaat
    15061 ggattatcca ccgcttaagg gtgcggcatt ggcgtgggtt acgctgtctt tggggcttgc
    15121 cgtatttatg gaagttttag atacgactat cgccaatgtc gccgttcccg tcatcgccgg
    15181 caacctcggt gcggcaacca cgcaggggac gtgggtcatt acttcttttg ctgtggcaaa
    15241 cgccgtttcc gtgccgttaa cgggcttttt ggcaaaacgt atcggcgagg tcaaattgtt
    15301 taccgccgcc gctgtcggtt tcgtcatcac atcgtggctg tgcggtattg cccccaacct
    15361 tcagtcgctg gttgttttcc gcatcttgca gggctttatc gccgggccgc tgattcccct
    15421 gtcgcaaagc ctgttaatgg catcctatcc gcccgcaaaa cggacgctgg cactggcatt
    15481 gtgggcaatg accgtcgttg tcgcccctgt tctcgggccg atactcggcg gttggatttc
    15541 cggaaactgg cattggggtt ggattttctt cattaatatc cctatcggta tcatatcggc
    15601 atggattaca tggaaacatt tgaaatatcg ggaaacggaa accgttaaaa tgccgaccga
    15661 ctatgtcggg cttacattga tggtagtcgg tatcggcgcg ttacagatga tgctggacag
    15721 gggtaaggaa ctcgactggt tcgcctctgg agaaatcatt accttgggcg tagtcgcact
    15781 ggtgtgtttg tcgtatttta ttgtttggga attgggagaa aaatatccga ttgtcgattt
    15841 atcgctgttt aaagatcgga attttaccgt cggcgtaatt gccacatcat tgggttttat
    15901 ggtgtatatg gggacgctga ccttgctgcc gttagtgttg cagaccaacc tgggctatac
    15961 ctccacgtgg gcggggcttg ccgccgcacc cgtcggcatc ctgcccgtct tcctgtctcc
    16021 gttaatcggc aggttcggca ataaaatcga tatgcgcctg ctcgtaaccg ccagcttcct
    16081 gacctttgcc tttactttct attggcgcac cgatttttat gccgacatgg atatcggcaa
    16141 cgtcatctgg ccgcagtttt ggcagggggc aggcgttgcc atgttcttcc tgccgctgac
    16201 caccatcaca ctgtcgcaca tgaagggcgg acagattgcc gccgcaggca gcctgtcgaa
    16261 tttcttgcgc gtgctgatgg gcggtgtcgg cgtatccgtc gtcagcaccc tgtgggaacg
    16321 gcgcgaagcg ttgcaccata cacgctttgc cgaacacatc acgccctatt ccgcaacatt
    16381 gcacgaaacg gccgctcatt tgtcccagca cggcgtttcc gacattcaaa ccctaggcat
    16441 catcaacaat accattaccc agcagggttt tattatcggc tcgaacgaaa tctttatggc
    16501 gggcagcttg ttattcatta tcatgatacc cgtcatatgg ctggcaaaac cgccgttcca
    16561 caacggcggc ggcggtggac attgagggat ttgaaaactt gaaatgccgt ctgaaaatac
    16621 tggaaatatg ttcggacggc attttgaatg cagcagttcc cgaaatccgc tataatcgcg
    16681 ccccatctgt ttcgcacctg caaacgttcc acagatgcga caatcggaag gattatccgc
    16741 gcaaaacagc cgtttttctc taaaacactt gaactaacac tgtttttcgt ggtataaatc
    16801 gcgttttact attttagaag tttggagact gattatggca cgagtttgca aagtgaccgg
    16861 caaacgcccg atgtccggca acaacgtatc gcacgccaac aacaaaacca aacgccgttt
    16921 tttgcccaac ttgcaatcac gtcgtttttg ggtagaaagt gaaaaccgct gggttcgcct
    16981 gcgcgtttcc aacgctgcac tgcgtaccat cgacaaagta ggcattgatg tcgtattggc
    17041 tgatttgcgt gctcgcggcg aagcttaatt taaacactat ttaattaagg attactgcaa
    17101 tgcgcgataa aatcaaactg gaatccagtg caggtactgg tcacttctac accactacca
    17161 aaaacaaacg cactatgccc ggcaaactgg aaatcaaaaa attcgatccg gttgcccgca
    17221 aacacgtagt gtacaaagaa accaaactga aataatttca gtttgaaagc aaagcctccg
    17281 actgttcgga ggctttttta tttttatcgt gtttcttttc cgcttgaaac atctgctgta
    17341 tgcgaatctg ctgcaaaccg tctgccaagg atagagaacg attctctaag gtgctgaagc
    17401 accaagtgaa tcggttccgt actatttgta ctgtctgcag cttcgtcgcc ttgtcctgat
    17461 ttttgttaat ccactataat tattttttag cgtgtaaaac aaaaccggct gcatacctgc
    17521 aaccggcctc aaatcagcac aattccttat ccaaatccgc caacagctct ttcagaccgt
    17581 ctccgatttc ctgaagcgta accggacggt tgccgtccga acccgactcc gcaccttctg
    17641 catacggtgc aaacgtgctc tgcaatttca acagtttcac cacatccaaa cgggtgtagc
    17701 gttcgccgcc gtaaccgaca accacaccat aatcatgctg ccattgcgca aaaccatagg
    17761 ggctgatttg caacagtccg cacaactcgt ccagcgtgaa atagcgtttt gcgggaacct
    17821 tcaaatcaac gttgtttgtc atagtagtgt tccaccatgc ttttgagttt ctggctggca
    17881 tggaaagtta ccacgcggcg ggcggtaatc ggcacttcct cgccggtttt aggattacga
    17941 cccgggcgtt gcggcttgtc gcgcaactgg aaatttccga aaccggaaat tttgatttct
    18001 tcgccgcttg ccaaagtgct gcggatttct tcaaaaaaga gttcgacgat ttctttggca
    18061 tcgtttttgg tgacgttgct gactttgtct accaaaatat cggccagttc tgctttagtg
    18121 agagtcatat gattaccttc tgttgtaaca gttgtggatt attaccaatt tttatttaaa
    18181 attcaagcga atatttattt tttaactgcg aagccgcgcc cctgccgccg ttgccgcgcc
    18241 aatcagtttt ccgataagcg gctcgaccgc ctcatccgtc agcgtgtttt ccatatcctg
    18301 caaaatcact ttgaccgcca cgctcttcat cccttcgggc agtcccatgc cgcgatacac
    18361 gtcaaacacg ctgatttcct gtaccaactt gtttgccgcg cctttcaaga caagcagcaa
    18421 atcatcatgg ctcatagctt ccggcatcac aaacgccaaa tcgcggcgca ccggctggaa
    18481 tttcgatacg gcccgatagc gcgttttccc gcattccaac acggccgcca tatcgatttc
    18541 aaataccagc ggcgcttgcg gcaggtcgta tttttgcagc catttcggat gcagttcgcc
    18601 gacaaagccg atgactttgc cgtctgaaac gatattggcg gcgcgtccgg gatgcagggc
    18661 gggatatccg gttttaacga actcgaccgc tttgtttttc aacagatttt ccacatctgc
    18721 cttgatgtcg taaaaatccg cattgcgcgt tttcccgccc cattgttcgg gcattgccgc
    18781 gccgtaccac aagccgccga tgcgttcgtt ttggacaaac cggccgtctg aacctttgcc
    18841 gaacacgcgg gcgatttcaa acacgcgcac gcggttttgt ttgcggttca ggttgttttg
    18901 caaaatttcc accaagccgc cgatgagtgt ggaacgcatc acggcatatt gcgccgccag
    18961 cgggttttgc aggcggatgg ggtcggcgtt ggcggcaaaa tcttgttccc actgctcgtc
    19021 aacgaaggca tagctgacca cttcgcggta gccacgcgcc gccatttcgt tgtaaacggc
    19081 aaaacgcgag cgacgtgttt cgggcagttc cagcattttc agacggcctg acgtgtaatc
    19141 gtcggggatg ttttcatagc cgtaaacgcg tccgatttct tcaatcaaat cagcctcaat
    19201 ttcgatgtca aaacggaagc tcggcgcggt aacgcggaag ccttccgccg ttttctcggg
    19261 ctgcagaccc aagtgttgca aaatggtttc cacctgttcg gcaggaatgt ccacgcccaa
    19321 cacggttttc agacggtcca aacgcaatcc aacctgcttc gcttcaggca attcgccttg
    19381 cgcttccacc atctcgcctg ccgcaccacc gcaaatctgc aacaccaatt cggtagcacg
    19441 ttcaatggca tccgcctgca aacggtaatc cacgccgcgc tcgaagcgga acgacgaatc
    19501 cgaaccgaaa ccgtattggc gcgatttgcc ggcgatgatt tcgggcgcaa accaagccgc
    19561 ttccagcacg atattttgcg tgccgtctga aaccgcgctc gccgcgccgc ccattaagcc
    19621 cgccaaactc aacgcgcctt tttcgtctgc cacgaccagc gtattttcag acagggaaac
    19681 ggttttctcg ttcaggcatt ccagcgtttc cccttcgcgc gcgcggcgga tgtgcaggct
    19741 gccggaaagt ttgtcggcat cgaaaacgtg catcggctgg ccgatttcca gcattacata
    19801 attgccgatg tccaccagca cggaaatact gcggatgccg ctgcgctcca aacgttgttt
    19861 catccaatcg ggagtagccg ctttcgcatt gacattttca ataacgcggc tgataaaacg
    19921 gccgcaatcg gcaggcgcgt taatctgcac gggctgtttt cgactgcccg tgatcggcgc
    19981 ggtatggatt tcgggctgcc tgaacgcgca ccccgtcaat gcggacactt cgcgcgcaat
    20041 gcccttaaca ctcaagcaat cagcgcggtt aggcgtaatt ttcaacgtaa acagcgtatc
    20101 gtccaaatcc aagtattcac ggatattgat accgacaggc gcgtcttcag gcaaaatgtg
    20161 caggccgttc acaccatcat caggcaaacc tagttcattg gtcgaacaca gcataccgtt
    20221 tgatggcacg ccgcgcattt tggtcggctt gattttgaaa ttacccggca aaacggcacc
    20281 cggcaacgaa cacggcactt tgatgcccgc tttcacattc ggcgcaccgc acacgatctg
    20341 aaccaactct cccgtacccg catcaactcg ggtaacgttc aaacggtctg catccggatg
    20401 tttttcaacg gatttcactt cggcaacaac cacgcccgca aaggcaggtg cggcagtttc
    20461 agcctcttcc acttccaagc cggacatcgt taacagatgt tccagcttat cggcggaaag
    20521 ttcggtatcg gcttgggttt tcagccatga gtaggagaat tgcatatttt tcctaaggat
    20581 taaatttgat tggacaactt aagggtaacc gagtgtaaat tacattacac atttttccat
    20641 aaagcagtct aaaaattctt cattgacagc tttatttgtc aatttccggc gaatgcacaa
    20701 ggaatacaac tcttcaactg cggaataagg aaaatattgg gtatctaaat ttttaaacat
    20761 cggaacattc gcttctttaa aaactttatc cctatcttca tcaggagcaa cgataaccca
    20821 gcgcgtatta gcatatggtg gcaacaggtc tttcaactgc ttcatccgtg caaggccgga
    20881 acgtacaccc gtactatgtt ccacttccat caccgccggc atcagttttc cattcctgaa
    20941 ccagatgcaa tcaatattca ttgccgcctg aaccgcgccg tcaaacgaac tgatcagttt
    21001 ctcgctttct atcttttgta cgataccatc caaatcagca attttcttat ccccataact
    21061 aataccttgg tcgtttctcg caatccacgt cctgaaacca agttgaaaac ctattgctga
    21121 aagggcaatc tggatttgca cgtggcggcg ttttacatct atatctattt ctgaatgggt
    21181 tgaaggaagg gataaagcat cgtaaaccac attgttggag gggatttccg aaataacgac
    21241 atctgtctca tattccttca ttacgccttt ttcatgtggc ctatcgggaa gccaaataat
    21301 gtgcttgtgc ccttccacca cctcgctttt attcagaccc ggttgtatcc gctgcaattt
    21361 gcaccagtaa atttcaggtg tatgggcaag caatgcctcc agtgcggaac gaaagttata
    21421 acttgcgcca aacaccctgt ccacattgac aggctccccc gtggacaacg catctgccaa
    21481 ccgccaaatt gcttgtttcg atatcgaagt ttccgtatct ccccttttaa tcttaatcgg
    21541 accttccggt ttttcaacat ggacgatttg tatggtatgc gtatttgagc tgttcacata
    21601 gttataattg acattttttt tcaagctatc aatcgcactg accaaattag cagccgtcag
    21661 tttttgtccg ctcatagtga ttctccaaat aagattttaa tgtcatcgct atcccttttg
    21721 ctgccaaaaa cggtacaccg ttgcctatag ttttgaacat attgctcaat gtcatgttat
    21781 caggcagctc aaactccttc ggcaaagact gtattgccaa tgcctccgca gcggaaatcc
    21841 ttcttggcag atagggatga atgtggactt cgttgtttcc gtatgcagca gtcggagaat
    21901 aacgccagcg gtgtaggcgt ttataggact tctttttatc atccccttcg cttatggttt
    21961 gaaatttttc taaacccgct cttggttgga agtgtttgct cgcattcggg tgtgtatcca
    22021 ccccattgcg attgaaccaa tactgaacgg tcaactcagg gtatatgttt gcaggctgac
    22081 tgaccactct tcctacttct tctgtcgtag gccaatcttt ttccaaggca tcagggaatg
    22141 aaatatgagc atcccaatca aacttctgca aagcatcaac atgctgactc aaaaaaccca
    22201 ctaggataat cctttccctg tcttgcggta cgccgtattc gatggcattg atgagttttt
    22261 cagtgcatac atatccaaaa tcagaaagtt gccgtttcaa cgcattgaaa aactccctat
    22321 gcttggcagt cctatatagt cctttcacat tctcaaatac aaaaaaatca ggctggtttt
    22381 tacaaattaa atccacataa ctttgtgata acttaccatt ctccccatcc ttacctttat
    22441 ttttccctgc aatagaaaaa tccggacatg ggggacctcc aataaatccg acaataatgc
    22501 cattttgttt ttccttttta acccagccag ccaaatcttt tgctttctca gcataaagac
    22561 attcgtcaat actaccctta aaatatccgt atttaggctt cgggatatcc attctgcttc
    22621 gagagtattt atatgcttcc aaaaatgggg gatggacttc attaacgaag gcaatatcaa
    22681 atcctgacaa ctcgaagccc aaatccaaaa agcccgcacc agagaaaaaa gaaaaaatca
    22741 aagttttttt tctttcctgc cgtatgctta tatccaaatt gcagctttta tccgtcaact
    22801 ggaaggggag attatttttc tctcgtttga aattttgaca atccctgttt gtcgcggacg
    22861 gcttgtctga ctttttcggc acagcaggtt gtatttttaa cgacatcatt actccaaaaa
    22921 cgtaaaaccg cccagccctc gaccttcaga cggcctgtta cttccatatc ccttcggata
    22981 ttgcgttcta tcttggcaat ccaaaattcg cggttgccct ttatttccgc ctttctctgt
    23041 tcccaatcct tgccgtgcca aaactcgcca tcaacaaaca cggcaacttt atatttttta
    23101 aatgaaaaat caggcttccc aaaaatgctt ccgctatttt tccgatacct gagccccaaa
    23161 gcccacatcg ccttcgccaa cgcaagctcg ggcttcgtcc ccttgctttt gttggactgc
    23221 atacattttt tgcgctgttc gggggttaat ctgtccattt cgtgaggcct gaaacgcgtt
    23281 tatacgaaag cgcattcatt ttacgcggca tcgttgcgga ttccggcagt tttgcacccc
    23341 ttgcaaggaa acaacaaaac ccaatcgcgc aggaagccac ctgtttcgtc aggttttccc
    23401 tgcaaagccg caaggttcgc agccggacgg actcaaaatt accaagcatc ccatccgcac
    23461 aaatcttcag aacgcatcaa ttcatctgcc gaaatcccgc cgaaatcttg ccgcaggctt
    23521 tttcagcccg tccggccgga caatatgcgg gttgcactcc gcttgcaggc tgcccgcacc
    23581 attatttcgc aaactgcttc aaaaaattca aatcgttatc aaaaaacaag cgcaaatcgt
    23641 tcacgttgta acgcagcatc gcgaaacggt cgagaccgat accgaaagcg aaaccggtgt
    23701 atttttcggg atcgatattc acatttctca acacgttggg atgcaccatg ccgcagccgc
    23761 cgacttccag ccatttgccg ttttcgccca taatgtcgat ttcgccagac ggttcggtga
    23821 acgggaagaa agacgggcgg aaacgtactt gcaaatcatc gcgttcaaag aagcgacgga
    23881 taaaatccgt gaacactgct tttaagtcgg caaaagttac accctcttct acccacaaac
    23941 cttccgcctg atggaacata ggcgagtgcg tggcatcgct gtccacacgg taaacgcggc
    24001 cgggggcgat aatgcggatg ggcggctctt ttttatcgag catatagcgg atttgaatcg
    24061 gggaagtgtg cgtacgcaaa acatcaccgt tttcaacgta aaacgtatcc tgcatcgcac
    24121 gggcaggatg gtttgcaggg atgttcaggg cttggaaatt gtgaaaatcg tcttcgattt
    24181 caggcccgtc cgccacttcg aaacccattc cgtgaaagag ttcgaccaca cgttgcaagg
    24241 tcagggttac gggatgcagg ctgccgcctt cctgagcgcg tccgggcagg gtaatgtcga
    24301 gggcttcggc ggcaagtcgg gcttgcagct tggcttcgtt gagggcatcg cgtttggcat
    24361 taaaagccgt ctgaaaccgg tttttgcatt cattgatatg cgcacctatg gttttgcgct
    24421 cttcaggcga catttgcccc aaagttttca gaagtccggt caactcgccg gttttgccaa
    24481 gataacgggc tttgatttgt tctagagcgt tgaagtcttg cgcagcttct actgcggcaa
    24541 tgccttctgc aacgatgcgg tttacatttt ccataatatc aagtctgtca attaatgata
    24601 tgccacctga aacaacgagg ccgcctgtat agcctgacat atcagactct tcagacgacc
    24661 ttattttctt cacggtcaat ccattggata aattttctgt atagtggatt aaatttaaac
    24721 cagtacggcg ttgcctcgcc ttgccgtact atccgtactg tctgcggctt cgtcgctttg
    24781 tcctgattta aatttaatcc actatatttt aacgcaaatt tatttaaaaa aatagacttt
    24841 aaagaaagaa aatcaatcac atagaatttc tgcaaacaaa gaaaaaagga agccgcagct
    24901 tccttttcaa ttttttggat taagcagcca aagcagattt ggctttttca accaattgtg
    24961 caaaagcggc tttatcgaac acggccaaat cagccaatac tttgcggtcg atttcaatag
    25021 aggcgcgttt cagaccgttc ataaatttgc tgtaagacaa cccgttttca cgcgcacctg
    25081 cattgatacg gacaatccac aattgacgga attggcgttt gcgttggcgg cggtcgcggt
    25141 atgcgtattg gccggctttc attaccgcct gcttggcaac gcggtaaacg tttttacgac
    25201 gaccgcggta gcctttggct aacgcgaaga ttttttggtg acgggcacgg gcggtaacac
    25261 cgcgttttac gcgtggcata ttctaaactc cttaagcgta gggtaacatt ttagcaacag
    25321 aagccaaatc gcgatcattt accatagagg taccgcgcag ttggcgtttg tttttggtgg
    25381 tctttttagt caagatgtgg cgtttgaacg catgagcgcg tttcacaccg ccgttaccca
    25441 gtactttaaa gcgttttttc gcgctagact tggttttcat tttaggcatg ggaaaactcc
    25501 attcgttatc ggataaggca ttagggggtt ttaaaacatc ggtttcaaac acttgaaccc
    25561 acaatgccac ggttttttgc cgcaattaaa aacttactcc gaagcggcga tcggagtaag
    25621 cggagaatta tagctttatt ttttcttcgg cgcaatcatc atcaccattt ggcgaccttc
    25681 cattttggga aaggactcga tttgcgccac ttcagccaaa tcttctttta cacgttccaa
    25741 aagttgcgcg ccgagttgct ggtgagccat ttcacggccg cgaaaacgca atgtcacttt
    25801 gactttatcg ccgtcggcaa ggaagcggtt aatgttgcgc atcttgattt gataatcgcc
    25861 ctcatccgta cccggacgga atttgatttc cttgatttgc acctgctttt gatttttctt
    25921 ggcttcgtcg cgcttcttgg cttgttggta tttgtattta ccgtaatcca tcagtttgca
    25981 cacaggcggt ttagcagttg gggaaatctc taccaaatcg acatcctgcc cttcggccat
    26041 agccaaagct tcacgaactg aaacgacacc aagctgttcg cctgactcac tgattaaacg
    26101 cacttctttg gcggtaattt cgccgttgat tcgtgcttcg cgttcttgag cgatgatgaa
    26161 tactcctata aaaattaatg attgacgagg gcatcagtga tttcttgctg caattgcgca
    26221 atgaaatcat ccaaatccaa agaacccaaa tcttctgctt tgcggcgtac cgccactttg
    26281 ttttcctgct tttctttatc gccgacaacg atttgataag gaaaacggta ttggctgttg
    26341 tcgcggattt tgtaaccgat tttttcgtta cgcaaatcca actcggcgcg gaatcctgcc
    26401 gcctgcaatt tggcagccac ttcacggcag taatccgctt gattttcggt gatattcata
    26461 atcaccattt gaaccggcgc cagccataac gggaaagagc ctgcatggtt ctcaatcaga
    26521 atgccgataa accgctccaa agaacccaaa atggcgcggt gcaacattac agggcgagcg
    26581 cggtcattgt tttcggttac atattctgca tccaagcgtt ccggtaagac gaaatctagt
    26641 tgcaatgtac cgcattgcca agaacgaccc aaggcatctt tgacgtgata ttcgatttta
    26701 ggcccgtaaa acgcaccttc gccaggcaac tcttcccact ctacgccaca agcggtcaat
    26761 gcatcacgca agccttgttc ggccttatcc caaacttcgt cagaacctgc acgttgttca
    26821 gggcggaggg acaacttcac agcaacatca tggaaaccga actgtttgta gatgcgaacc
    26881 aacaattcat tgaatgcgcg cgcttcgctg acgatttggt cttcggtaca gaaaatatgc
    26941 gcatcatctt gcacaaaacc gcgaacacgc atcagaccat gcaatgcacc gcttggttca
    27001 ttgcggtggc aagaaccgaa ctcggccaaa cgcatcggca aatcgcgata tgagcgcaaa
    27061 ccgttattaa aaatttgcac atgacccgga cagttcatcg gtttcaccgc gtattcgcgt
    27121 ttttctgaac tggtcacgaa catattgtct ttgtagttgt cccaatggcc ggatttttcc
    27181 caaaaggttt tatccataat ttgaggcgtt ttgacctctt tataaccggc ggcgttcagc
    27241 tctttacgca tatgctgttc aatcacttgc cacaaagccc agcctttagg atgccaaaac
    27301 accatgcccg gcgcttcgtc ttgcagatgg aacagatcca attgcttgcc aagtttgcgg
    27361 tggtcgcgct tttcggcttc ttcgatacgt tgaatatagg cttttaattc gtcttttgtc
    27421 gcccaagccg taccgtatat gcgttgcagc atttcattat tgctgtcgcc gcgccagtat
    27481 gcgcccgcca acttggtcag tttgaagttt ttcaggaaac gggtattcgg aacgtgcggt
    27541 ccgcggcaca tatcgacata ttcctgatga tgatacatcc ccatcgcttc cacttcgggc
    27601 atatcgtcaa tcaggcgcag tttgtattct tcgccgcgct cttgaaaaat tttaatcgcc
    27661 tccgcacgcg gagtcatgat tttgaccaca tcatagtctt gggcaatcaa ttctttcata
    27721 cgcgcttcaa tggcggcaac atcttccggt gtaaacggtt tttccgtggc gatgtcgtaa
    27781 taaaagccct cttcaatgac ggggccgata accatttttg cattaggata gagttgcttg
    27841 acggcatgcc cgacaagatg ggcgcaggaa tggcggatga tttcgatgcc ttcctgatct
    27901 ttcggagtaa tgatttgaac agcagaattt tcaacaatcg ggtcgcacgc atcgaccaat
    27961 ttgccgttta ccctgcctgc caccgccgcc ctcgccaaac cgacaccgat agacgcagca
    28021 atttgagcca cggtaacggg ggattcgtat tggcggactg agccgtccgg caaggtaatg
    28081 ttcaacatca aacgctccga aagattaaaa aataacggca taaatgccgt tatgggaatt
    28141 tggtaggcac gattggattc gaaccaacga cccccaccat gtcaaggtgg tgctctaacc
    28201 aactgagcta cgtgccttca aagaattttt gcattttatc gggtcgtttt aatttttgca
    28261 agaggtatgt gcatttcttt atgcaacatt gtggcaagat ttggggatgt cgtctgaaaa
    28321 accgtgggaa ttcatttata atgccgtctt ttaaaacata tttcgacccc tgtcgcccaa
    28381 ctgacattca atcttatgct taaatttacc ttacacaaaa aagacggata cgcccgacgc
    28441 ggcacgctgg agttgaacca cggcaaaatc gaaacgccgg tatttatgcc tgtcggtact
    28501 tacggctcgg ttaaggcgat gaacccgcaa aacctgcacg acatcaaggc gcaaattatt
    28561 ttgggcaaca cttatcattt gtggctgcgt ccgggcttgg aggtcgtcga acagtttgga
    28621 gggcttcacg gctttatcgg ctgggacaaa ccgattctga ccgactcggg cggttttcag
    28681 gttttttctt tgtcggatat gcgcaagctg acggaagaag gctgtacgtt caaaagcccg
    28741 atcaacggcg acaagctgtt tttatcgcct gaaatttcca tgaaaatcca aacggttttg
    28801 aactcggaca tcgcgatgca gctggacgaa tgcacgccgg gcgaagcaac gcgcgaacag
    28861 gcgaaaaaat cgctgcaaat gagcctgcgc tgggcggaac ggagcaaaaa agcctttgaa
    28921 gatttgaaaa acccgaacgc gctgttcggc atcgtgcaag gcgcgatgta tgaggatttg
    28981 cgcgaagaat cgctgcgcgg tttggaacag tttgattttc ccggccttgc cgtcggcggt
    29041 ttgtccgtcg gcgaacccaa gcccgaaatg taccgtatgc tgcgcgccgt cggtccgatt
    29101 ctgccggaac acaagccgca ttacctgatg ggcgtaggca cgccggaaga cctcgtgtac
    29161 ggcgtggcac acggcatcga tatgttcgac tgcgtgatgc ccacccgcaa tgcacgcaac
    29221 ggctggctgt ttacccgttt cggcgatttg aaaatcaaaa acgccaaaca caagctcgac
    29281 aagcgtccga tagacgaaag ctgcacctgc tacgcctgcc aaaacttcag ccgcgcctac
    29341 ctgcaccatc tgcaccgcac cggcgaaatc ttgggcgcac agttgaacac catccacaat
    29401 ctgcatttct accaagtcat tatggcggaa atgcgcgaag ccgtcgaaca aggcaaattt
    29461 gccgactggc aggcgcgatt ccacgaaaac cgcgcacgcg gcacagactg attccctgca
    29521 atgccgtctg aagccgcttc agacggcatt ttgccgcttt accctacccg cactattttt
    29581 cagaaaccga tcatgcttcc attctttcca gaaccctccc tttcctatac gcaacaaaac
    29641 cgtaccgccg ttttgctgtt gaacctcggc acgcccgacg cgccgaccgc gcaggcggta
    29701 cgaccttatc tgaagtcctt tctgaccgac cggcgcgtcg tcgaactgcc caaatggctg
    29761 tggtatccca ttctgcatgg tctggtactg acgctccgcc ccaaaaaaag cgcgcacgcc
    29821 tatgaaaaaa tctggtttaa ggaaggttcg cccttagaag tttacacagc acgccaagct
    29881 gccgcgcttg ccaaacgtat gcccgacctc atcgtccgcc acgccatgac ctacggcaat
    29941 ccttcggtgg cagacgtatt gtcggaactg aaggcgcaag gcgcgggcag actgctggtc
    30001 atcccgatgt acccgcaata tgccgcttcc agcagcggag cggcagtaga taaggtgtgc
    30061 gaacaattac tgctgcaacg gaaccaaatg agcgtccgca ccgtttcgcg cttttatgac
    30121 gataccggct acattgatgc gatgaaaaac cacatcctcc gatattgggc ggaacacggg
    30181 cgcgggaaaa aactgatgtt gagttttcac ggcgtaccgc agaagcacca cgacctcggc
    30241 gacccctatc ccgacgagtg ccgccacacc gccaaactgc ttgccgaagc actggaactg
    30301 accgaagacc aatatgtcgt atcgttccaa agccaattcg gcagggcaaa atgggttaca
    30361 ccgagcacgc aggatttgtt cggcaaactg cccaaacagg gggtaaccga gctggacgta
    30421 ttttgcccgg gctttttggc agactgtttg gaaaccatgg aagaaatcgc cctgatgggg
    30481 cgggaacagt tttacgaagc aggcggcaaa agctaccgct acatcccctg cctcaacgac
    30541 aaccccgact ggatagacgc actcgtcgca cttgccgaag aaaaccttgg cggctggcgt
    30601 tgaaactgtt tgacccatgt ctaaaatgcc gtctgaacgc cgtgttcaga cggcattttc
    30661 ccgctacttt agcgtatgat ggcatctatg ccggacaaca acatcaagtg caaccttact
    30721 accgattcgg caaaaaaatc tttcacccgc tacgcaaaat caacaacaag gtttcaaaca
    30781 ttaaggaaac accaatgaac acaacccgac tgccgaccgc cctcgtcttg ggctgcctct
    30841 gcgccgccgc ttctgccgcc gacaacagca tcatgacaaa agggcaaaaa gtgtacgaat
    30901 ccaactgcgt cgcctgccac ggcaaaaagg gcgaaagccg cggaaccatg tttccgccgc
    30961 tctaccgctc cgacttcatc atgaaaaaac cgcaggtgct gctgcacagc atggtcaaag
    31021 gcatcaacgg tacaatcaaa gtcaacggca aaacctacaa cggattcatg cccgcaaccg
    31081 ccatcagcga tgcggacatt gccgccgtcg ccacttatat catgaacgcc tttgacaacg
    31141 gcggcggaag cgttaccgaa aaagacgtaa aacaggcaaa aggcaaaaaa aactaaacag
    31201 acaaaatgcc gtctgaaacc ggcaatccgg cttcagacgg cattcaaatc aaacctttct
    31261 aacgcggtac tttcagcccc atagacccga tattgccgtc cccgtcaacc tccttcgcgg
    31321 tcagcataaa gccgccgatg tcaatacggc cgcactcgac cggctcctga ctgtcggaac
    31381 gcttatcgaa aagctcggca aggctcaaac cctcttcgcc cgcttccagc ctcaaaccgt
    31441 aagcaagtgc caaatcaccc gaacgtgctg ccggcaaaac gacaaactca ccgaagaaat
    31501 caaaatactc acggacgcta atacccgtct cggtaaagta tttcgccatt ttatagtgaa
//
LOCUS       DAWFAD010000006        28466 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWFAD010000006 DAWFAD010000000
VERSION     DAWFAD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 28466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.006"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            24..1106
                     /locus_tag="WGO49_000196"
     CDS             24..1106
                     /locus_tag="WGO49_000196"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ0305360.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
     gene            1221..1493
                     /locus_tag="WGO49_000197"
     CDS             1221..1493
                     /locus_tag="WGO49_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ0305361.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWFGKACQNGDQDGCDIYQRLKAGY"
     gene            1583..2920
                     /locus_tag="WGO49_000198"
     CDS             1583..2920
                     /locus_tag="WGO49_000198"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ0305362.1"
                     /translation="MTLENKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATKDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGKKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            3046..4371
                     /locus_tag="WGO49_000199"
     CDS             3046..4371
                     /locus_tag="WGO49_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ0305363.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGLLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            4375..5442
                     /gene="murG"
                     /locus_tag="WGO49_000200"
     CDS             4375..5442
                     /gene="murG"
                     /locus_tag="WGO49_000200"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ0305364.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPVTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDDARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            5596..7005
                     /locus_tag="WGO49_000201"
     CDS             5596..7005
                     /locus_tag="WGO49_000201"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ0305365.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            7118..8032
                     /locus_tag="WGO49_000202"
     CDS             7118..8032
                     /locus_tag="WGO49_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ0305366.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRNDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAESCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            8022..8750
                     /locus_tag="WGO49_000203"
     CDS             8022..8750
                     /locus_tag="WGO49_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ0305367.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            8836..10080
                     /gene="ftsA"
                     /locus_tag="WGO49_000204"
     CDS             8836..10080
                     /gene="ftsA"
                     /locus_tag="WGO49_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ0305368.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            10199..11377
                     /gene="ftsZ"
                     /locus_tag="WGO49_000205"
     CDS             10199..11377
                     /gene="ftsZ"
                     /locus_tag="WGO49_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ0305369.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            11516..12826
                     /locus_tag="WGO49_000206"
     CDS             11516..12826
                     /locus_tag="WGO49_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ0305370.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            13452..14327
                     /gene="prpB"
                     /locus_tag="WGO49_000207"
     CDS             13452..14327
                     /gene="prpB"
                     /locus_tag="WGO49_000207"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ0305371.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            14413..15567
                     /gene="prpC"
                     /locus_tag="WGO49_000208"
     CDS             14413..15567
                     /gene="prpC"
                     /locus_tag="WGO49_000208"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ0305372.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            15660..16382
                     /locus_tag="WGO49_000209"
     CDS             15660..16382
                     /locus_tag="WGO49_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEZ0305373.1"
                     /translation="MDKDKGSYAGIPVQNTKELVWQKIQDIPISECGEPLVSVGNTGR
                     IRVNPVYFSQGIEGAINSVLLRRSVADRLTQVVSLLPEVYGLEIFDGWRPVIVQRALR
                     ENMKQEIEKRHPEYDEVQVREALDCFAADPDRAGMPLPHLTGGSVDVGLFDIKSGKSL
                     DMGSEFDATGHLSYSDALEKEGEYFSSARNLRRILIHAMKEAGFSNLPTEWWHFDYGN
                     QNWAYFTQQSNAVFGAVEEQDT"
     gene            16408..17613
                     /locus_tag="WGO49_000210"
     CDS             16408..17613
                     /locus_tag="WGO49_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HEZ0305374.1"
                     /translation="MQTLISADNHLLLWAFVMLAAAAAIFIEQNSRLAGKVPGAVLAL
                     LIALIASNLGVIPTDAPVFDAVWSYIVPLAIPLLLFQLDLHALFKESGRMLFIFLISS
                     LGTVIGTLVSFALLKNYIPELDKVAGMISASYTGGGVNFAAMSAKLNPAKDVIAATIV
                     ADNLMMACYFFILIGISSSYWVRKVWGSPYQDKLEQEYHDPSVNKAAEYWKPKSISLQ
                     SIALSLAASILLVAVSFSLSSFLQNHFQSVDGVALKMLTGLISDKYLLLTTFTLAVVL
                     FFRKGIQKLDGSQELGTYCIYLFFVVIGVPASIPLIIKTAPLLFVFTLIIAVLNLLLT
                     LVLGKLFKFSIEEIVLACNANIGGPTTAAALAISKGWRDLVGAILLIGTVGYIIGNYI
                     GSFVYALLS"
     gene            17716..20322
                     /gene="acnD"
                     /locus_tag="WGO49_000211"
     CDS             17716..20322
                     /gene="acnD"
                     /locus_tag="WGO49_000211"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ0305375.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRKAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATVDLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLSGMRPLAILPDNITTDHLSPSNAILASSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVRNEDGSVRQGSLARVEPEGQTMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPGTNRH
                     TLQLDGTETYDVVGERKPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            20675..21844
                     /gene="prpF"
                     /locus_tag="WGO49_000212"
     CDS             20675..21844
                     /gene="prpF"
                     /locus_tag="WGO49_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ0305376.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            22259..23458
                     /locus_tag="WGO49_000213"
     CDS             22259..23458
                     /locus_tag="WGO49_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0305377.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSIVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELAGIL"
     gene            23565..24185
                     /locus_tag="WGO49_000214"
     CDS             23565..24185
                     /locus_tag="WGO49_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ0305378.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRRGCGDTAVFGL"
     gene            complement(24312..24755)
                     /locus_tag="WGO49_000215"
     CDS             complement(24312..24755)
                     /locus_tag="WGO49_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ0305379.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLATPPDRINIGSVVRHLEPMQLVECMGSNNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            24929..25297
                     /locus_tag="WGO49_000216"
     CDS             24929..25297
                     /locus_tag="WGO49_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0305380.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEETKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            25342..26496
                     /locus_tag="WGO49_000217"
     CDS             25342..26496
                     /locus_tag="WGO49_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0305381.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(26553..27425)
                     /locus_tag="WGO49_000218"
     CDS             complement(26553..27425)
                     /locus_tag="WGO49_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ0305382.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            27510..28322
                     /locus_tag="WGO49_000219"
     CDS             27510..28322
                     /locus_tag="WGO49_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ0305383.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTISAGRAVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRTRAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         6565 a         7236 c         7906 g         6759 t
ORIGIN      
        1 acaaaacaaa aaaaggaagc cccatgtttt tatggctcgc acatttcagc aactggttaa
       61 ccggtctgaa tatttttcaa tacaccacat tccgcgccgt catggcggcg ttgaccgcct
      121 tagcgttttc cctgatgttc ggcccgtgga cgatacgcag gctgaccgcg ctcaaatgcg
      181 ggcaggcagt gcgtaccgac ggtccgcaaa cccacctcgt caaaaacggc acgccgacga
      241 tgggcggttc gctgattctg accgccatta ccgtgtccac cctgttgtgg ggcaactggg
      301 caaacccgta tatctggatt ctcttgggcg tattgctcgc cacgggcgca ctcggttttt
      361 acgacgactg gcgcaaagtc gtctataaag accccaacgg cgtgtccgcc aaattcaaaa
      421 tggtgtggca gtcaagcgtt gccattatcg ccagtttggc attgttttac cttgccgcca
      481 attccgccaa caatattttg attgtcccgt tcttcaaaca aatcgccctg ccgctgggcg
      541 tggtcggctt tttggtgttg tcttacctga ccatcgtcgg cacatccaat gccgtcaacc
      601 tcaccgacgg cttggacggc cttgcgacct tccccgtcgt cctcgttgcc gccggcctcg
      661 ccatcttcgc ctatgccagc ggccactcac aatttgccca atacctgcaa ttaccttacg
      721 ttgccggcgc aaacgaagtg gtgattttct gtaccgccat gtgcggcgcg tgcctcggtt
      781 tcttgtggtt taacgcctat cccgcgcaag tctttatggg cgatgtcggt gcattggcat
      841 tgggtgccgc gctcggtacc gtcgccgtca tcgtccgcca agagtttgtc ctcgtcatta
      901 tgggcggatt atttgtcgta gaagccgtat ccgttatgct tcaggttggc tggtataaga
      961 aaaccaaaaa acgcatcttc ctgatggcgc ccatccatca ccactacgaa caaaaaggct
     1021 ggaaagaaac ccaagtcgtc gtccgctttt ggattattac catcgtcttg gtgttgatcg
     1081 gtttgagtac cctcaaaatc cgctgaacct atgccgtctg aacacctttc agacggcatt
     1141 tgaacgcgca ataaacctgc ggcgacaatc cgcccagccc tatcgttaac ggcggcttat
     1201 actaaaacag aagtaaaacc atgaaacaga cagtcaaatg gcttgccgcc gccctgattg
     1261 ccttgggctt gaaccaagcg gtgtgggcgg atgacgtatc ggattttcgg gaaaacttgc
     1321 aggcggcagc acagggaaat gcagcagccc aatacaattt gggcgcaatg tattacaaag
     1381 gacgcggcgt gcgccaagac cttgtgctcg ctcaagaatg gtttggtaag gcttgtcaaa
     1441 acggagacca agacggctgc gacatttacc aacgcctgaa ggcaggttat tgaacagccc
     1501 gcgatgccgt ctgaaagcgg cttgggcagg ggcggacatc cccccacctg cccaatatga
     1561 tttgttttag gacaaaacca aaatgacgct tgaaaacaaa aaaatcctcg tcgccggact
     1621 cggcggtacg ggtatttcca tgattgccta tctgcgcaaa aacggcgcgg aggtggccgc
     1681 ttatgatgcg gagttgaagc ccgagcgcgt gtcgcaaatc ggtaagatgt ttgacgggct
     1741 ggtgttttac acaggccgtc tgaaagatgc gctggacaac ggtttcgata ttttggcact
     1801 cagccccggc atcagcgagc gtcagccgga tattgaggcg ttcaagcaaa acggcggacg
     1861 cgtgttgggc gacatcgaat tgctggcgga cattgtgaac cgccggggcg acaaggtaat
     1921 tgcgattacc ggcagcaacg gcaaaaccac ggtaacgagc ctggtcggct atctctgtat
     1981 caagtgcggg ctggataccg ttatcgcggg caatatcggc acgccggttt tggaggcgga
     2041 atggcagcgc gaaggcaaaa aggcggacgt gtgggtgttg gagctttcca gcttccaact
     2101 ggaaaacacc gaaagcctgc gcccgacggc ggcgacggta ctgaatattt ccgaagacca
     2161 tctcgaccgc tacgacgact tgctcgacta cgcgcatacc aaagccaaga ttttccgtgg
     2221 cgacggtgtg caggttttga atgcggacga tgcgttctgc cgtgccatga aacgtgccgg
     2281 gcgcgaggta aaatggtttt cgttggaaca cgaagccgat ttttggttgg aacgtgagac
     2341 aggccgcctg aaacaaggca atgaagattt gattgccacg aaagacattc cgttgcaagg
     2401 cttgcacaac gccgccaacg tcatggctgc cgtggctttg tgcgaagccg tcggtttgcc
     2461 gcgcgaagca ttgcttgaac acgtcaaaac cttccaaggc ctgccgcacc gcgtggaaaa
     2521 aatcggcaag aaaaacggcg tggtattcat tgatgacagc aaaggcacga atgtcggcgc
     2581 gaccgccgcc gcgattgccg gtttgcaaaa tccgctcttc gtgattttag gcggcatggg
     2641 caaagggcag gacttcacgc ctctgcgcga cgcgcttgcc ggcaaggcaa aaggcgtgtt
     2701 cctgatcggc gttgatgcgc cgcaaatccg ccgcgatttg gacggctgcg gtctgaatat
     2761 gaccgactgc gccactttgg aagaagccgt tcagacggca tatgcccaag ccgaagcgtg
     2821 cgatatcgtg ctgctcagcc cggcttgtgc gagttttgat atgtttaaag gctacgcgca
     2881 ccgttcggaa gtgtttatcg aagcgtttaa ggctttgtga tgccgtctga aatgcaaacg
     2941 ccgtcattgt tgggcggcaa gtaaagattt agaataccga tttgggatgt atcgtatgtt
     3001 cggacggcat tgtctgccgt ctgaaatttt tgccctttgc ggcaggtgca aacagactgg
     3061 caggtggttt ttttgaagat ttcggaagta ttggtaaaag tgggcgacgg tgtccacact
     3121 ctgctgctcg acaggccgat tgtgcgcgac ggcaggaaat tcgacgcgcc gcttttgtgg
     3181 atggtggtgc tgatgacggc gttcagcctg ctgatgattt attcggcttc tgtgtatttg
     3241 gcatcaaaag aaggcggcga tcagtttttc tatttgacca gacaggcggg gttcgtcgtt
     3301 gccggcttga tagcgagcgg tttgttatgg tttctttgca ggatgaggac atggcggcgg
     3361 cttgtgccgt ggatttttgc cctatccggc ctgttgctgg tagtcgtatt gattgccggg
     3421 cgcgaaatca atggcgcgac ccgttggata cctttgggtc cgttgaattt ccagccgacc
     3481 gagctgttca agctggcggt catcctttat ttggcaagcc tgttcacgcg ccgtgaagaa
     3541 gtgttgcgca gcatggaaag tttgggttgg cagtcgattt ggcgggggac ggccaatctg
     3601 atcatgtccg ccaccaatcc gcaggcacgt cgtgaaacat tagaaatgta cggccgtttc
     3661 cgggcgatca tcctgccgat tatgctggtg gcgttcggtt tggtgctgat aatggtacag
     3721 ccggatttcg gttcgtttgt cgtcattacc gtcattgccg ttggaatgct gtttttggca
     3781 ggattgccgt ggaaatattt tttcgtcctg gtaggcagcg tcttgggcgg gatggtgctg
     3841 atgattaccg ccgctcccta ccgtgtgcag cgggtagtgg catttttgga cccgtggaaa
     3901 gacccgcagg gtgccggcta ccagcttacc cactctctga tggcaatcgg gcgcggagag
     3961 tggttcggta tgggtttggg tgcgagtttg agcaaacgcg gctttctgcc ggaagcgcat
     4021 accgatttta tttttgccat catcgccgaa gaattcggtt tcttcggtat gtgcgtgctg
     4081 atattctgtt acggctggct ggtggtgcgg gcgttttcca tcggcaagca gtcgcgcgat
     4141 ttgggtttga ctttcaacgc ctatatcgct tcgggtatcg gcatttggat cggtatccaa
     4201 agtttcttca atatcggtgt gaacatcggt gctttgccga ccaaaggtct gacgctgccg
     4261 ttgatgtcct atggcggttc gtcagtcttt ttcatgctga tcagcatgat gctgctgttg
     4321 cgtatagatt atgaaaaccg ccggaaaatg cgcggttatc gggtggagta aatcatgggc
     4381 ggtaaaacct ttatgctgat ggcgggcgga acgggcggac atattttccc cgcgctggcg
     4441 gtggcggatt cattgcgcgc gcgcggccat catgtgattt ggctgggcag caaggattcg
     4501 atggaagagc gtatcgtgcc gcaatacggc atacgcttgg aaacgctggc gattaaaggc
     4561 gtgcgcggca acggcatcaa acgcaaactg atgctgccgg ttactttgta tcaaaccgtc
     4621 cgcgaagcgc agcggattat ccgcaaacac cgtgtcgagt gcgtcatcgg cttcggcggc
     4681 ttcgttacct ttcccggcgg tctggcagcg aaactcttgg gcgtgccgat tgtgattcac
     4741 gagcaaaacg ccgtggcagg cttgtccaac cgccacctgt cgcgctgggc gaagcgggtg
     4801 ttgtacgctt ttccgaaagc gttcagccac gaaggcggct tggtcggcaa ccccgtccgc
     4861 gccgatatta gcaacctgcc cgtgcctgcc gaacgcttcc aagggcgtga aggccgtctg
     4921 aaaattttgg tggtcggcgg cagtttgggc gcggacgttt tgaacaaaac cgtaccgcag
     4981 gcattggctt tgctgcccga cgatgcgcgt ccgcagatgt accaccaatc gggacggggc
     5041 aagctgggca gcttgcaggc ggattacgac gcgctgggcg tgaaagccga atgcgtggaa
     5101 tttattaccg acatggtgtc cgcctaccgc gatgccgatt tggtgatttg ccgtgccggc
     5161 gcgctgacga ttgccgagtt gacggcggcg ggattgggtg cgttgctcgt tccgtacccc
     5221 cacgcggttg acgatcacca aaccgccaac gcgcgtttta tggtgcaggc agaagcagga
     5281 ctgctgttgc cgcaaaccca gttgacggcg gaaaaactcg ccgaaatcct cggcagcctg
     5341 aaccgcgaaa aatgtctcaa atgggcagaa aacgcccgta ctttggcatt gccgcacagc
     5401 gcggacgacg ttgccgaagc cgcgattgcg tgtgcggcgt aaactgccga accatgccgt
     5461 ctgaaaagcc gttcagacgg catggatgtt ttttatttca atccgctata tatttgtcag
     5521 aaaactatgg cgcgcaaacg gtcagccctt taaaataacg cctttacgca tcgaaatcca
     5581 ccggaacgca acattatgat gaaaaatcga gtgaccaaca tccattttgt cggtatcggc
     5641 ggcgtcggca tgagcggtat cgccgaagtc ttgcacaatt tgggttttaa agtttccggt
     5701 tcggatcagg cgcgaaatgc cgctaccgag catttgggca gcctgggcat tcaagtttat
     5761 cccggccata ccgcagaaca cgttaacggt gcggatgtcg tcgttacctc taccgccgtc
     5821 aaaaaagaaa atcccgaagt tgtcgctgcg ttggagcagc aaattcccgt tattccgcgc
     5881 gccctgatgt tggcggagtt gatgcgcttc cgtgacggca tcgccattgc cggcacgcac
     5941 ggcaaaacca cgaccaccag cctgaccgcc tccatcctcg gcgcggcagg acttgacccg
     6001 actttcgtta tcggcggcaa actcaacgcc gcaggcacca acgcccgctt gggcaaaggc
     6061 gaatacatcg ttgccgaagc cgacgagtcg gatgcatcct ttctgcacct gacaccgatt
     6121 atgtccgtcg ttaccaatat cgacgaagac catatggata cctacgggca cagcgtcgaa
     6181 aaactgcatc aggcgtttat cgatttcatc caccgtatgc ccttctacgg caaagccttt
     6241 ttgtgtattg acagcgaaca cgtccgcgcg attttgccca aagtgagcaa accttatgct
     6301 acttacggtt tggacgatac cgccgacatc tacgccaccg acatcgaaaa cgtcggcgcg
     6361 caaatgaaat tcaccgtcca tgttcaaatg aaaggacatg agcaggggtc gtttgaagtc
     6421 gtgctgaata tgcccggcag acacaacgtg ctgaacgcat tggcagccat cggcgtggcg
     6481 ctggaagtcg gcgcatcggt tgaagcgatc caaaaaggct tgctcggctt tgaaggtgtc
     6541 ggccgccgct tccaaaaata cggcgacatc aagttgccaa acggcgggac cgcgctcttg
     6601 gtggacgact acggacacca ccccgtcgaa atggcggcga cccttgccgc cgcacgcggc
     6661 gcgtatccgg aaaaacgttt ggtactcgcc ttccagccgc accgctatac ccgcacgcgc
     6721 gatttgtttg aagactttac caaagtcctc aataccgttg acgcgctggt gctgaccgaa
     6781 gtttatgccg ccggtgaaga gccgattgcc gccgccgatt cccgcgctct tgcccgcgcc
     6841 atccgcgtgt tgggcaaact cgagccgatt tactgcgaaa acgttgccga tctgcccgaa
     6901 atgctgttga acgttttgca ggacggcgac atcgtgttga atatgggtgc gggaagcatc
     6961 aaccgcgtcc ccgccgcgct gctggaattg tcgaaacaga tttgaggcac acccgcctga
     7021 cagacggaac atcatataaa ggtcgtctga aaccgcaaat caggtttcag acgacctctg
     7081 gcaacaagca taaagcaatc aggaaagaac aaaaacaatg cagaattttg gcaaagtggc
     7141 cgtattgatg ggcggttttt ccagcgaacg agaaatctcg ctggacagcg gcaccgccat
     7201 tttgaatgct ttaaaaagca aaggcataga cgcatatgcc ttcgatccta aagaaacccc
     7261 attgtctgaa ttgaaggcac aaggttttca gacggcattc aacatccttc acggtactta
     7321 cggcgaagac ggggcggttc agggtgcatt ggaactgttg ggcattccct ataccggcag
     7381 cggtgtcgcc gcatccgcca tcggcatgga caaataccgc tgcaaactga tttggcaggc
     7441 attgggactg cccgttcccg agttcgccgt cctgcacgac gacactgatt tcgatgccgt
     7501 cgaagaaaaa ttgggcctgc cgatgtttgt gaaaccggcg gccgaaggca gcagcgtagg
     7561 cgtggtaaaa gtcaaaggaa aaggccgtct gaaaagcgtt tacgaagaat tgaaacacct
     7621 tcagggcgaa atcattgccg aacggtttat cggcggcggc gaatattcct gccccgtctt
     7681 gaacggcaaa ggcctgcccg gcatacacat catccccgcg accgagtttt atgactacga
     7741 agccaagtac aaccgcaacg acaccattta tcaatgtcct tcggaagatc tgaccgaagc
     7801 cgaagaaagc ctgatgcgcg aactggcggt tcgcggcgcg caggcaatcg gtgcggaaag
     7861 ctgcgtgcgc gtcgatttcc tcaaagatac cgacggcaaa ctctatctgt tggaaatcaa
     7921 caccctgccc ggtatgacga gccatagttt agtaccgaaa tccgctgccg ttacgggcgt
     7981 gggttttgcc gatttatgta ttgaaatttt gaagaccgca catgtgggat aatgccgaag
     8041 cgatggaacg gctgacgcgc tggctgcttg tcatgatggc gatgctgctt gctgcgtccg
     8101 ggctggtttg gttttacaat tcgaatcatc tgcccgtcaa gcaggtgtcg ctgaagggca
     8161 acctggttta ttccgataag aaggcattgg gcagtttggc gaaagaatac atccatggga
     8221 atattttgag gacggacatc aatggcgcac aggaggccta ccgccggtat ccgtggattg
     8281 cgtcggtcat ggtgcgccgc cgttttcccg acacggttga ggtcgtcctg accgagcgca
     8341 agccggtcgc gcgttggggc gaccatgcct tggtggacgg cgaaggcaat gtttttgaag
     8401 cccgcttgga cagacccgga atgccggtat tcagaggcgc ggaaggaacg tctgccgaaa
     8461 tgctccgccg ttatgacgaa ttttcgactg ttttggcaaa acagggtttg ggcatcaaag
     8521 agatgaccta tacggcacgt tcggcgtgga ttgtcgtttt ggacaacggc atcaccgtca
     8581 ggctcggacg ggaaaacgag atgaaacgcc tccggctttt taccgaagcg tggcagcatc
     8641 tgttgcgtaa aaataaaaat cggttatcct atgtggatat gaggtataag gacggatttt
     8701 cagtccgcta tgcttccgac ggtttacccg aaaaagaatc cgaagaatag tgggaacagg
     8761 tatcggacag attacgaccg tgccgtctga aacggtgcga cgcaaatttc aatcagtttt
     8821 aagagcagac gaacaatgga acagcagcaa agatacatca gcgtactgga tatcggtacg
     8881 tctaaagtcc tcgcactgat cggggaagtt caagatgacg acaaaatcaa catcgtcggt
     8941 ttggggcagg ctccttcacg gggcttgcgc gcgggcatgg taaccaatat cgatgccacc
     9001 gtccaagcca tcaggcaggc ggtcaatgat gccgagctga tggcggatac caaaattacc
     9061 cacgttacca caggtatcgc aggcaaccac atccgcagtc tcaattcgca aggtgtggtt
     9121 aaaattaaag acggggaagt cacgcaggca gacatcgacc gcgccattga aacggcaaag
     9181 gcaatcaata tcccgcccga tcaaaaaatt ctcgatgccg tggttcaaga ctacattatc
     9241 gacacccaac ttggcgtgag ggagcccatc ggtatgagcg gtgtgcgtct ggatacgcgg
     9301 gtgcacatca ttaccggtgc aagtacggca gtgcagaatg tccaaaaatg tatcgagcgg
     9361 tgcggtttga aaagcgatca gatcatgctt cagccgttgg caagcgggca ggcggtgctg
     9421 actgaagatg aaaaagacct cggcgtatgc gtcatcgaca ttggtggcgg aacgaccgat
     9481 attgccgttt atatgaacgg tgccatccgc catacgtccg tcattccggc cggtggtaat
     9541 ctgattacca aagatttgtc caaatcgttg agaacacctc tcgatgccgc cgagtacatt
     9601 aaaatccatt atggcgtggc atcatgcgat acggaaggct tgggtgagat gattgaagtt
     9661 ccgggcgtgg gtgaccggac atcgcgtcag gtttccagta aggttttggc agcaatcatc
     9721 agcgcacgta ttcaggagat ttttggcgta gtgctgggcg agctgcaaaa atcgggtttc
     9781 cccaaagaag tgctgaatgc gggtatcgtt ctgaccggcg gtgtgtccat gatgaccggg
     9841 attgtggaat ttgccgaaaa aatcttcgat ttgcctgtac gcaccggtgc gccccaagaa
     9901 atgggcggtt tgtccgaccg cgtccgcaca ccgcgctttt ctaccgccat cgggctgctt
     9961 catgcagcat gcaagctgga aggaaacttg ccgcagccgg aaaacggtgc agtgcaagag
    10021 agggaagggg gcggcggttt gttggcaaga ttgaaacggt ggattgaaaa cagcttctga
    10081 acaggtggat tgccgtttga caggtgagaa gtattttgcc agcagcaaga tacttcttat
    10141 ataatgaata ataatttatt taaaccgtcc tctgaatggg gcgagcagga gtttttgaat
    10201 ggaatttgtt tacgacgtgg cagaatcggc agtcagccct gcggtgatta aagtaatcgg
    10261 cttgggcggc ggcggttgca atgcaatcaa taacatggtt gccaacaatg tgcgcggtgt
    10321 ggagtttatc agtgccaata cggatgcgca gtctttggca aaaaaccatg cggcgaagag
    10381 aatccagttg ggtacgaatc tgacacgcgg tttgggcgcg ggcgcgaatc ccgatatcgg
    10441 ccgtgcggca gcccaggaag accgggaagc cattgaagaa gccattcgcg gtgcgaatat
    10501 gctgtttatc acgaccggta tgggcggcgg taccggtacc ggttccgcgc cggttgttgc
    10561 tgagattgcc aagtctttgg gcattctgac cgttgccgtg gttacccgac cgttcgcata
    10621 tgaaggtaag cgcgtccatg tcgcacaggc agggttggaa cagttgaaag aacacgtcga
    10681 ttcgctgatc atcatcccga acgacaaact gatgactgca ttgggtgaag acgtaacgat
    10741 gcgcgaagcc ttccgtgccg ccgacaatgt attgcgcgat gcggtcgcag gcatttccga
    10801 agtggtaact tgcccgagcg aaatcatcaa cctcgacttt gccgacgtga aaaccgtgat
    10861 gagcaaccgc ggtatcgcta tgatgggttc gggttatgcc caaggtatcg accgtgcgcg
    10921 tatggcgacc gaccaggcca tttccagtcc gctgctggac gatgtaacct tggacggagc
    10981 gcgcggtgtg ctggtcaata ttacgactgc tccgggttgc ttgaaaatgt ccgagttgtc
    11041 cgaagtcatg aaaatcgtca accaaagcgc gcatcccgat ttggaatgca aattcggtgc
    11101 ggctgaagac gagaccatga gcgaagatgc catccggatt accattatcg ctaccggtct
    11161 gaaagaaaaa ggcgcggtcg attttgttcc ggcaagggag gtagaagcgg ttgctccgtc
    11221 caaacaggag caaagccaca atgtcgaagg tatgatccgc accaatcgcg gtatccgcac
    11281 gatgaacctt accgctgcgg atttcgacaa tcagtccgta cttgacgact ttgaaatccc
    11341 tgcgattttg cgtcgtcaac acaattcaga caaataatgt gctgtttgcc cgtaaacctg
    11401 ctgcctcccg aatcggtttg tccggtttgg gaggtatgtt tttcaagatg ttgcaatttc
    11461 gtacggtttg cggtcggcgg attcagattt ttccacttga tacagacttt cagatatgga
    11521 cacttcaaaa caaacactgt tggacgggat ttttaagctg aaggcaaacg gtacgacggt
    11581 gcgtaccgag ttgatggcgg gtttgacaac ttttttgacg atgtgctaca tcgttatcgt
    11641 caaccctctg attttgggcg agaccggcat ggatatgggg gcggtattcg tcgctacctg
    11701 tatcgcgtct gccatcggct gttttgttat gggttttgtc ggcaactatc cgattgcact
    11761 cgcaccgggg atggggctga atgcctattt cacctttgcc gtcgttaagg gtatgggcgt
    11821 gccttggcag gttgcgttgg gtgcggtgtt catctccggt ctgattttta tcctgttcag
    11881 cttttttaaa gtcagggaaa tgctggtcaa cgcactgcct atgggtttga aaatgtcgat
    11941 tgctgccggt atcggtttgt ttttggcact gatttccctg aaaggcgcag gcattatcgt
    12001 tgccaatccg gcaaccttgg tcggtttggg cgatattcat cagccgtccg cgttgttggc
    12061 actgttcggt tttgctatgg tggtcgtatt gggacatttc cgcgttcaag gcgcaatcat
    12121 catcaccatc ttgaccatta ccgtcattgc cagcctgatg ggtttgaatg aatttcacgg
    12181 catcatcggc gaagtaccga gcattgcgcc gacttttatg cagatggatt ttgaaggcct
    12241 gtttaccgtc agcatggtca gtgtgatttt cgtcttcttc ttggtcgatc tatttgacag
    12301 taccggaacg ctggtcggca tatcccaccg tgccgggctg ctggtggacg gtaagctgcc
    12361 ccgcctgaaa cgcgcactgc ttgcagactc taccgccatt gtggcaggtg cggctttggg
    12421 tacttcttcc accacgcctt atgtggaaag cgcggcgggc gtatcggcag gcggacggac
    12481 cggcctgacg gcggttacag tcggcgtatt gatgctcgcc tgcctgatgt tttcaccttt
    12541 ggcgaaaagt gttcccgctt ttgccaccgc gcccgccctg ctttatgtcg gcacgcagat
    12601 gctccgcagt gcgagggata ttgattggga cgatatgacg gaagccgcac ccgcattcct
    12661 gaccattgtc ttcatgccgt ttacctattc gattgcagac ggcatcgcct tcggcttcat
    12721 cagctatgcc gtggttaaac ttttatgccg ccgcaccaaa gacgttccgc ctatggtatg
    12781 gattgttgcc gtattgtggg cactgaaatt ctggtatttg ggctgattga ttcgatatta
    12841 aaaatgccgt ctgaaaggtt ttcagacggc attttgtttg ccgatatatt taatttttat
    12901 taaattatat aaaaatcaaa tacataataa aatacatcgg attgcttaaa aataatacat
    12961 tgtttttatg tataaaatat tttataagtt ttcaggattt tgattatcga aaatttttct
    13021 tgatttcctg acaattttat tgaaacaaat aattcaaaat taatctagtt taatcatgga
    13081 attaaaataa aatattaaaa ttatgtaatg agtctcctta aaaatgtttg acattttcag
    13141 tcttgtgttt tagattatcg aaaaataaaa ctacataaca ctacaaagga acattactat
    13201 gaaaccaatt cagatgtttt ccccttttct gaataatccc cttgttttct tcttgtctgc
    13261 ggttttgccg cataattccg aacggtctgc tgtttttctt tgattcgttt taaatatcaa
    13321 taagataatt tttcccatat atttttaatg attggattgg gatgcccgac gcgtcggatg
    13381 gctgtgtttt gccgtccgaa tgtgatggaa gcctgtccat actgaaaaaa agtctataaa
    13441 ggagaaatat gatgagtcaa cactctgccg gagcacgttt ccgccaagcc gtgaaagaat
    13501 cgaatccgct tgccgtcgcc ggttgcgtca atgcttattt tgcacgattg gccacccaaa
    13561 gcggtttcaa agccatctat ctgtccggcg gcggcgtggc agcctgttct tgcggtatcc
    13621 ctgatttggg cattaccaca atggaagatg tgctgatcga cgcacgacgc attacggaca
    13681 acgtggatac gcctctgctg gtggacatcg atgtgggttg gggcggtgca ttcaatattg
    13741 cccgtaccat tcgcaacttt gaacgcgccg gtgttgcagc ggttcacatc gaagatcagg
    13801 tagcgcaaaa acgctgcggc caccgtccga acaaagccat tgtatctaaa gatgaaatgg
    13861 tcgaccgtat caaagctgcc gtagatgcgc gcgttgatga gaacttcgtg attatggcgc
    13921 gtaccgatgc gctggcggta gaaggtttgg atgccgctat cgaacgcgcc caagcttgtg
    13981 tcgaagccgg tgcggacatg attttccctg aagccatgac cgatttgaac atgtaccgcc
    14041 aatttgcaga tgcggtgaaa gtgcccgtgt tggcgaacat taccgagttt ggttccactc
    14101 cgctttatac ccaaagcgag ctggctgaaa acggcgtgtc gctggtgctg tatccgctgt
    14161 catcgttccg tgcagcaagc aaagccgctc tgaatgttta cgaagcgatt atgcgcgatg
    14221 gcactcaggc ggcggtggtg gacagtatgc aaacccgtgc cgagctgtac gagcatctga
    14281 actatcatgc cttcgagcaa aaactggata aattgtttca aaaatgattt accgctttca
    14341 gacggtcttt caacaaatcc gcatcggtcg tctgaaaacc cgaaacccat aaaaacacaa
    14401 aggagaaata ccatgactga aactactcaa accccgacct tcaaacctaa gaaatccgtt
    14461 gcgctttcag gcgttgcggc cggtaatacc gctttgtgta ccgttggccg taccggcaac
    14521 gatttgagct atcgcggtta cgacatcttg gatttggcac aaaaatgcga gtttgaagaa
    14581 gtcgcccacc tgctgattca cggtcatctg cccaacaaat tcgagctggc cgcttataaa
    14641 accaagctca aatccatgcg cggcctgcct atccgtgtga ttaaagtttt ggaaagcctg
    14701 cctgcacata cccatccgat ggacgtaatg cgtaccggcg tatccatgct gggctgcgtt
    14761 catcctgaac gtgaaagcca tccggaaagt gaagcgcgcg acatcgccga caaactgatc
    14821 gccagcctcg gcagcatcct cttgtactgg tatcaatatt cgcacaacgg caaacgcatt
    14881 gaggttgaaa gcgacgaaga gaccatcggc ggtcatttcc tgcaactgtt gcacggcaaa
    14941 cgcccaagcg aatcacacat caaagccatg cacgtttcac tgattctgta tgccgaacac
    15001 gagttcaacg cttctacctt taccgcccgc gtgatcgccg gtacaggctc tgatatgtac
    15061 tccagcatta ccggagcaat cggcgcgttg aaaggtccga aacacggcgg cgcgaacgaa
    15121 gtggcttacg atattcaaaa acgctaccgc aatgccgacg aagctgaagc cgacatccgc
    15181 gaacgcatcg gccgcaaaga aatcgtgatc ggtttcggtc atccggtgta taccatttcc
    15241 gacccccgca acgttgtcat taaagaagtg gcacgcggtt tgagcaaaga aaccggcgat
    15301 atgcgcctct ttgacattgc cgaacgtttg gaaagcgtga tgtgggaaga gaaaaaaatg
    15361 ttcccgaatc tggactggtt ctctgccgtt tcctaccaaa aattgggcgt accgaccgct
    15421 atgttcacac cgctgttcgt aatttcccgt acaaccggtt ggagcgcaca cgttcttgag
    15481 caacgcaaag acggcaaaat catccgtccg agcgcaaact acacgggccc tgaagatttg
    15541 gcgtttgtgg agattgaaga acgctgatga ttttcagacg accttgtttg tacaaggtcg
    15601 tctgaaaaaa gaaatttaat gaatttattg gcacactaat tattaatgcg gaacatttga
    15661 tggataagga caaggggtct tatgcgggta ttcctgtaca aaataccaaa gagttagttt
    15721 ggcagaaaat acaagacata cctatttctg aatgtgggga accgttggtc agtgttggta
    15781 ataccggacg cattcgtgtc aatcctgttt atttttctca gggtattgaa ggggcgataa
    15841 atagtgtttt attgcgtaga agtgttgctg ataggctgac tcaggttgtc tctttgctgc
    15901 cggaagtgta cgggttggaa atttttgatg gttggcgtcc tgtcatagtg caaagagcat
    15961 tacgagaaaa tatgaagcaa gaaattgaaa aacgccatcc ggaatatgat gaagttcaag
    16021 ttagagaagc actggactgt tttgctgctg atccagatcg ggcaggaatg cctctgcccc
    16081 atttgacagg cggatctgtt gatgtcggat tgtttgatat aaaaagtggc aaaagtttgg
    16141 atatgggaag tgagtttgat gcaactggac atttatctta tagcgatgct ttagagaaag
    16201 aaggtgagta tttctcctct gctcgtaatt tacgccggat tttgattcat gcgatgaaag
    16261 aggcaggatt tagtaatttg ccaaccgaat ggtggcattt cgattatggc aaccaaaatt
    16321 gggcttattt tacccaacaa agcaacgcag tttttggggc ggtagaagag caggatactt
    16381 aaattaaaat aaaaattggg aggaaaaatg caaactttaa tttcagcaga taatcatttg
    16441 ctgctgtggg cttttgttat gttagctgcc gctgctgcga tttttattga gcaaaacagt
    16501 cgcttggctg ggaaagtccc tggtgcagta ttagctttat tgattgcgtt gattgcttct
    16561 aatttgggtg ttattccgac ggatgctcca gtatttgatg ctgtatggtc ttatattgta
    16621 cctcttgcta ttccgttgtt attgtttcaa ttagatcttc atgctctatt taaggaaagt
    16681 ggacgtatgc tgtttatttt cctgattagt tcactaggga cggtcattgg aacgcttgtc
    16741 tcatttgcac tattgaaaaa ctatattcct gaactagata aggtagctgg catgatttca
    16801 gcatcttata cgggaggtgg tgttaatttt gcagctatgt cagctaagct taaccctgct
    16861 aaagatgtta ttgcagcaac aattgttgct gataatttga tgatggcttg ttactttttt
    16921 attttgattg gtatctcttc ctcatattgg gtcagaaaag tttggggaag tccatatcag
    16981 gataaattgg agcaggaata tcatgatccg agtgtgaata aggctgccga gtattggaaa
    17041 cctaaatcca tttcgctaca gagtatagcc ttatctttgg ctgcctcgat tttgttggtc
    17101 gcagtttcat tctcattatc cagttttttg caaaatcatt ttcaaagtgt tgatggtgtt
    17161 gcattaaaaa tgctgacagg tttaattagt gacaaatatt tgctgttaac gacatttaca
    17221 ttggcagttg ttttattttt ccgtaaaggc attcaaaaac tagatggcag tcaagaatta
    17281 gggacatatt gtatttatct gttttttgtt gtgattggtg ttcctgcctc tataccgtta
    17341 attattaaaa cagcaccatt actctttgtt tttactttga ttattgcggt attgaatttg
    17401 ttactgacgt tagtgttagg caagctgttc aaattcagta ttgaagaaat tgtattggct
    17461 tgtaatgcca atattggagg gccgactact gcagcagcct tagccatcag taaaggttgg
    17521 cgtgatttag tcggtgcaat tttgttaatt ggtactgtag gctacattat cggtaactat
    17581 ataggttcgt ttgtatatgc cctattgagc tgaagactaa tctaacaatc catgaacatt
    17641 ttcagacgac ccccttccaa aaacgtcggc tgaaacctca aaccacaaga aaaacagatc
    17701 cccaggagaa ccgacatggc tgccaaccaa cgttaccgca aaccgctgcc cggtacggat
    17761 ttggaatact acgacgcgcg tgcggcgtgt gaggacatca agcccggctc ttacgacaag
    17821 ctgccttata caagccgtat cttggctgag aacttggtca accgcgcgga caaagtcgat
    17881 ttgccgacgc tgcaaagctg gctgggccag ttgatagaag ggaagcagga aatcgacttt
    17941 ccgtggtatc ccgcgcgggt ggtgtgccac gatattctgg ggcagaccgc gctggtggat
    18001 ttggcgggct tgcgcgatgc gattgccgaa aaaggcggcg atcctgccaa agtgaatccg
    18061 gtggtgcaaa cccagcttat cgtcgaccac tctctggcgg tggagtgcgg cggttacgat
    18121 cctgatgcct tccgcaaaaa ccgcgaaatc gaagatcgac gcaacgaaga ccgtttccac
    18181 ttcatcaact ggacaaaaac cgcgtttgaa aatgtggacg tgattccggc gggcaacggc
    18241 atcatgcacc aaatcaatct agaaaaaatg tcgccggttg ttcaagttaa aaacggcgtg
    18301 gctttccccg atacctgcgt cggcacggat tcgcacacgc cgcacgtcga tgcgctgggc
    18361 gtgatttccg tgggcgtggg cggattggaa gcggaaaccg taatgctggg acgcgcgtcc
    18421 atgatgcgcc tgcccgatat tgtcggcgtt gagctgaacg gcaaacggaa ggcgggcatt
    18481 acggcgacgg atattgtgtt ggcactgacc gagtttctgc gcaaagaacg cgtggtcggg
    18541 gcgtttgtcg aattcttcgg cgagggcgcg agaagcctgt ctatcggcga ccgcgcgacc
    18601 atttccaaca tgacgccgga gttcggcgcg actgccgcga tgttcgctat tgatgagcaa
    18661 accattgatt atttgaaact gaccggacgc gacgacgcgc aggtgaaatt ggtggaaacc
    18721 tacgccaaaa ccgcaggctt gtgggcagat gccttgaaaa ccgccgttta tcctcgcgtt
    18781 ttgaaatttg atttgagcag cgtaacgcgc aatatggcag gtccaagtaa cccgcatgcc
    18841 cgttttgcca ccgtcgattt ggcggcgaaa gggctggcga agccttacga agagccttca
    18901 gacggacaaa tgcccgacgg cgcggtcatc atcgccgcga ttaccagttg caccaacact
    18961 tccaacccgc gcaacgtcgt tgccgccgcg ctcttggcgc gcaacgccaa ccgcttcggg
    19021 ctgaaacgca aaccgtgggt caaaacctcg tttgcccccg gttcgaaagt ggcggaaatt
    19081 tatttgaaag aagcaggcct gctgcccgaa atggaaaaac tcggcttcgg tatcgtcgcc
    19141 ttcgcctgca ccacctgcaa cggcatgagt ggcgcgctgg atccgaaaat ccagaaagaa
    19201 atcatcgacc gcgatttgta cgccaccgcc gtattatcag gcaaccgcaa cttcgacggc
    19261 cgtgtccatc cgtatgcgaa acaggctttc ctcgcttcgc ctccgttggt cgttgcctac
    19321 gcgctggcag gcagtatccg tttcgatatt gaaaacgacg tactcggcgt tgcagacggc
    19381 aaggaaatcc gcctgaaaga catttggcct gccgatgaag aaatcgatgc cgtcgttgcc
    19441 gaatatgtga aaccgcagca gttccgcgat gtgtatgtac cgatgttcga caccggcaca
    19501 gcgcaaaaag cacctagtcc gctgtacgat tggcgtccaa tgtccaccta tatccgccgc
    19561 ccaccttact gggaaggcgc attggcaggg gagcgcacat tgagcggtat gcgtccgctg
    19621 gcgattttgc ccgacaacat caccaccgac cacctctcgc catccaatgc gattttggca
    19681 agcagtgccg caggcgaata tttggcgaaa atgggtttgc ctgaagaaga cttcaactct
    19741 tacgcaaccc accgcggcga ccacttgacc gcccaacgcg caacctttgc caatccgaaa
    19801 ctgtttaacg aaatggtgag aaacgaagat ggcagtgtac gccaaggttc gctggcacgc
    19861 gttgaacccg aaggccaaac catgcgcatg tgggaagcca tcgaaaccta tatgaaccgc
    19921 aaacagccgc tcatcatcat tgccggtgcg gactacggtc aaggctcaag ccgcgactgg
    19981 gctgcaaaag gcgtacgcct cgccggtgtg gaagcgattg ttgccgaagg cttcgagcgt
    20041 atccaccgta ccaacttgat cggtatgggc gtattgccgc tgcagttcaa accgggtacc
    20101 aaccgccaca ccttgcaact ggacggtacg gaaacctacg acgtggtcgg tgaacgcaaa
    20161 ccgcgctgcg atctgaccct cgtgattcac cgtaaaaacg gcgaaaccgt cgaagttccc
    20221 gttacctgcc gcctcgatac tgcagaagaa gtattggtat atgaagccgg cggcgtgttg
    20281 caacggtttg cacaggattt tttggaaggg aacgcggctt agaggtcgtc tgaaaagcaa
    20341 gacgtagcgt gggtcgggtt caacattttg ctcattcacg taattctcga tatggcaggc
    20401 atctactgta aatcgtcatt cccgcgcagg cgggaatcca gaaagtggaa ttgaggaaac
    20461 cttatttatc cgatgagttt ctgtgcggac aaatttggat tcccgcctgc gcgggaatga
    20521 cggggtttaa taatctgccg tatcacaaca cagtagccgt agattgtggc gaaccccgac
    20581 agtttgcgga atcaaacggc tttggtcgga gtggcagcct aatccactat aaaaatcgtg
    20641 ggcagagccc acgctacata aggagaatct agaaatgccg caaattaaaa ttcccgccgt
    20701 ttactaccgt ggcggtacat caaaaggcgt gtttttcaaa cgttccgacc tgcccgaggc
    20761 ggcgcgggaa gcgggcagcg cacgcgacaa aatcctcttg cgcgtactcg gcagcccgga
    20821 tccctacggc aagcagatag acggtttggg caacgccagc tcgtccacca gcaaggcggt
    20881 gattttggac aagtccgaac gcgccgatca cgatgtcgat taccttttcg ggcaagtttc
    20941 catcgacaaa ccttttgtcg attggagtgg caactgcggc aacctcaccg ccgccgtggg
    21001 cgcatttgcc atcgagcaag gcttggtcga taaaggcaag attccttcag acggcatctg
    21061 cacagtcaaa atctggcaga aaaacatcgg caaaaccatt attgcccatg taccgatgca
    21121 aaacggcgca gttttggaaa caggcgattt tgagctcgac ggcgtaacgt tcccggcagc
    21181 cgaagtacaa atcgaatttc ttgatccagc cgacggcgaa ggcagtatgt tcccaaccgg
    21241 caatttggtc gatgaaattg atgtgccgaa tataggccgt ttgaaagcca cgctcatcaa
    21301 cgcgggcatt ccgaccgttt tcctgaatgc cgccgacttg ggctacacgg gcaaagagtt
    21361 gcaagacgac atcaacaacg atgccgcagc tttggaaaaa ttcgagaaaa tccgcgctta
    21421 cggtgcgctg aaaatgggtc taatcagcga cgtatccgaa gctgccgccc gcgcgcacac
    21481 gccgaaagtc gccttcgtcg cgcccgccgc cgattacacc gcctccagtg gcaaaaccgt
    21541 gaatgccgcc gacatcgatt tgctggtacg cgccctgagc atgggcaaat tgcaccacgc
    21601 gatgatgggt accgcctctg ttgccattgc gaccgccgcc gccgtgcccg gtacgctggt
    21661 caaccttgcc gcaggcggcg gaacgcgtaa agaagtgcgc ttcgggcatc cttccggcac
    21721 attgcgcgtc ggtgcagccg ccgaatgtca ggacggacaa tggacggcca ccaaagcggt
    21781 tatgagccgc agcgcacgcg tgatgatgga aggttgggtc agggtgccgg aagattgttt
    21841 ttaaattgac gtagcatggg tttgcccgcg agccataaac aggtcgtctg aaaaacaagt
    21901 aaacatcaaa tcactgacca ttcctttccc ttgccctgtg gcggaaggcg gcaaatcaca
    21961 aggaagaaca cggaaacccc gataaaagac agcttcccgt attaccgtca ttcccgcgca
    22021 ggcgggaatc cagacctgtc aatatggagg attggcaggg gaaaacaggt ttcgtgagtt
    22081 ctacattctg gattcccgcc acagcctgtc ctcgcgtagg cggggacgga ataacgatag
    22141 aaaatgcggc atatgctttg cccaaagagg ccgtctgaaa cacactccgc ccaacgccca
    22201 tcctttttca gacgacccca caccaaaaaa acaaccacaa actacaagga gaaacatcat
    22261 gtccgaccaa ctcattcttg ttctgaactg cggcagttca tcgctcaaag gcgccgttat
    22321 cgaccgcaaa agcggcagca tcgtcctaag ctgcctcggc gaacgcctga ccacgcccga
    22381 agccgtcatt acgttcagca aagacggcaa caaacgccaa gttcccctga gcggccgaaa
    22441 ttgccacgcc ggcgcggtgg gtatgctttt gaacgaactg gaaaaacacg gtctgcacga
    22501 ccgcatcaaa gccatcggcc accgcatcgc ccacggcggc gaaaaataca gcgagtctgt
    22561 tttgatcgac caggccgtaa tggacgaact caatgcctgc attccgcttg cgccgctgca
    22621 caaccccgcc aacatcagcg gcatcctcgc cgcgcaggaa catttccccg gtctgcccaa
    22681 tgtcggcgtg atggatactt cgttccacca aaccatgccg gagcgtgcct acacttatgc
    22741 cgtgccgcgc gagttgcgta aaaaatacgc tttccgccgc tacggtttcc acggcaccag
    22801 tatgcgttac gttgcccctg aagccgcacg catcttgggc aaacctctgg aagacatccg
    22861 catgattatt gcccacttag gcaacggcgc atccattacc gccatcaaaa acggcaaatc
    22921 cgtcgatacc agtatgggtt tcacgccgat cgaaggtttg gtaatgggta cacgttgcgg
    22981 cgacatcgat ccgggcgtat acagctatct gacttcccac gccgggatgg atgttgccca
    23041 agtggatgaa atgctgaaca aaaaatcagg tttgctcggt atttccgaac tttccaacga
    23101 ctgccgcacc ctcgaaatcg ccgccgacga aggccacgaa ggcgcgcgcc tcgccctcga
    23161 agtcatgacc taccgcctcg ccaaatacat cgcttcgatg gctgtggcct gcggcggcgt
    23221 tgacgcactc gtgttcaccg gcggtattgg cgaaaactca cgtaatatcc gagccaaaac
    23281 cgtttcctat cttgatttct tgggtctgca catcgacact aaagccaata tggacaaacg
    23341 ctacggcaat tcgggtatta tcagcccgac cgattcttct ccggctgttt tggttgtccc
    23401 gaccaatgaa gaactgatga ttgcctgcga cactgccgaa cttgccggca tcttgtagcc
    23461 aaaaaaggga cgagtccgca caatgccgtc tgaaattttc agacggcatt ttgtttggat
    23521 tttgggcgta caatacggaa aacatgacga taaggacaca aaccatggca cagtttttcg
    23581 ctattcatcc cgacaatccc caagaacgcc tcatcaagca ggcggttgaa atcgtcaatc
    23641 aaggcggcgt ggtggtttat ccgaccgatt cgtgttacgc cttgggttgc aaactcggcg
    23701 ataaggcggc gatggaacgc atactctcca tccgcaaaat cgatttgaaa caccacctga
    23761 ccctgatgtg cgccgatttg agcgagttgg gcacgtacgc caaaattgac aacgtacagt
    23821 ttcgtcagct taaagccgcc acgcccgggc cttatacctt tatcttgcag gcgacgaagg
    23881 aagtgccggc gcgcacgctg cacccgaaac gcaaaaccat cgggctgcgt attcccgata
    23941 atgccattgc acaagccctg ctgggggaat tgggcgagcc gcttttaagc tgcaccctga
    24001 tgctgcccga agacggcgaa ccattgaccg atccttatga aatccgcgag cgtttggaac
    24061 acgccgtcga tttggtgatt gacggcggct ggtgcggaac cgagccgacc accgtcgtcg
    24121 atatgaccga cggcacggaa ctggtgcgcc ggggttgtgg cgatacggcg gtgttcggtt
    24181 tgtagggaaa ccgatgccgt ctgaagcatc ggctgttcag acggcattgc gcgccttgcc
    24241 ggcggcagtc cgaaatgccg gcgcgtatcg cgctcggtcg gaatatccgt ttgaaacggc
    24301 attttgatgc attactgcac cgcaatcgga attctcggtt cgtagagcag gtcgtaggtc
    24361 ggcttgttga gcaggtcttg gagcgtgaaa ccgtccagat acgtgaaaaa cgacttcatt
    24421 gcaccgccga gtatgcctgt cagacggcag gacggtgtaa tcaggcattc gttgttcgaa
    24481 cccatacatt cgaccagctg catcggttcg aggtggcgga cgaccgagcc gatgttgatg
    24541 cggtcgggcg gtgtggcaag ccgcagaccg ccgccttttc cgcgcacgct gtggaggaag
    24601 ccgcctttga ccagcgcggt aacgaccttc atcagatggc ttttggaaat gccgtaggtt
    24661 acggcgatgg tactgatgtt gaccagcgca tcgtcgttga tggcagtgta gataaggacg
    24721 cgcagcccgt agtccgtatg ttgtgtcaaa tacatgattt tctcggtatg gattgttatt
    24781 cttatcggta cggtttaagg ttcacggaca ataccttaat ggttgaaacc ctgtccgtcg
    24841 gggcggtaga atgcagcctg tctgcggcgg tatgccgtct gaaacatccg cgctaccgtt
    24901 tgagaatttg ttattgtaac tcaaaatcat gaaaccgttg aaacgacatc ccgcccttat
    24961 cgggctttcg cgtgaccacc accattcgct ttccctgtgc gtgcgtctgt tgcggacgcc
    25021 ggaagaaagg catcgggacg aactcgaacc gcatttttcc gaattggaaa cccattttcg
    25081 cgaagaagaa accaagtttg ccccaatttg gcagaatgtc gcccccgaat tgaaacaacg
    25141 tttcgagaaa gaccacgccc gactgcggca gatgatggca agccccgaat acggtaacgc
    25201 ggcgtggaat accgcttttg ccacaaccct gcgcgaccac gcgcgctttg aagaacgcga
    25261 gctgtttccc gccgccgaac cgtttttgcc ggcatgattc cgttttgcgg caaatatatt
    25321 aatgataaac aaggaacaca catgaaattt accaagcacc ccgtctgggc aatggcgttc
    25381 cgcccgtttt attcgctggc ggctctgtac ggcgcattgt ccgtattgct gtggggtttc
    25441 ggctacacgg gaacgcacga gctgtccggt ttctattggc acgcgcatga gatgatttgg
    25501 ggttatgccg gactggtcgt cgtcgccttc ctgctgaccg ccgtcgccac ttggacgggg
    25561 cagccgccca cgcggggcgg cgttctggtc ggcttgacta tcttttggct ggctgcgcgg
    25621 attgccgcct ttatcccggg ttggggtgcg tcggcaagcg gcatactcgg tacgctgttt
    25681 ttctggtacg gcgcggtgtg catggctttg cccgttatcc gttcacagaa tcaacgcaac
    25741 tatgttgccg tgttcgcgct gttcgtcttg ggcggcacgc atgcggcgtt ccacgtccag
    25801 ctgcacaacg gcaacctagg cggactcttg agcggattac agtcgggctt ggtgatggtg
    25861 tcgggtttta tcggtctgat tggtacgcgg attatttcgt tttttacgtc caaacggttg
    25921 aatgtgccgc agattcccag tccgaaatgg gtggcgcagg cttcgctgtg gctgcccatg
    25981 ctgaccgcca tgctgatggc gcacggcgtg atgccttggc tgtcggcggc tttcgcgttt
    26041 gcggcaggcg tgatctttac cgtgcaggtg taccgctggt ggtataagcc tgtgttgaaa
    26101 gagccgatgc tgtggattct gtttgccggc tatctgttta ccggattggg gctgattgcg
    26161 gtcggcgcgt cttatttcaa acccgctttc ctcaatctgg gtgtgcatct gatcggggtc
    26221 ggcggtatcg gcgtgctgac tttgggcatg atggcgcgta ccgcgctcgg tcatacgggc
    26281 aatccgattt atccgccgcc caaagccgtt cccgttgcgt tttggctgat gatggcggca
    26341 accgccgtcc gtatggttgc cgtattttct tccggcactg cctacacgca cagcatacgc
    26401 acctcttcgg ttttgtttgc actcgcgctt ttggtgtatg cgtggaagta tattccttgg
    26461 ctgattcgtc cgcgttcgga cggcaggccc ggttgagaca aaccgccgca gatttcgggt
    26521 ctgggcggtt tgcttttcag acggcagggc ggtcagttgc cgtccagcca gcggtcgcgt
    26581 gtggttttgg cttcttcaaa atagcggtac agggcttcgc ggtcgtcggt ggtcaggatg
    26641 tttgccaaaa cgtccaactg tttgcccaag ccttgaacca gttgcagcag gctgtctttg
    26701 ttggcaaggc agatgtccgc ccacacggcg ggatggccgg aggcgatgcg ggtgaagtcc
    26761 cgaaagcccg tggcggcgaa tttcagatat tcctgtccgt cggggtggtc gagaatctgg
    26821 tggacatagg cgaaggcggt caggtggggc atatgggaga cggcggcgaa aaccgcgtcg
    26881 tggcgttgcg cgtccatcgt ataaatttcc gcaccgaccg cgtgccacag ttcttctacc
    26941 aaggcaatgc cgtctgaatg ttcgccgccg tgtggcgtga tgatgagttt tctgtggcgg
    27001 aacagcccga actgcgcggc ttgcgcaccg cttctgtccg aaccggcaat cgggtgggcg
    27061 gcgatgcagt ggtgcagacg gtcgggcaga cagcggcgga aggcttcgat gaccgaagat
    27121 ttggtgctgc cgacatcgga aatccaagtg tgttccggca aaacggggcg cagcgcggtc
    27181 aaaatggcgg gaacggtggc gacgggcgtg gcaatcagta ccaagtccgc accgccgatg
    27241 ctgtccgcgt cgatggcaac ggaagcccgg tcaatcacgc cgcgttccaa tgcacgttcg
    27301 aggttgtcgc ggtcggtgtc gataccggta acggtgcgga cgagtccctg ccttttgagg
    27361 tcgagaacga acgaaccgcc gatcagccct acaccgatga gggcaatatg gttcaaaatg
    27421 ggcatttgtg taaacggttt tcgcaaagta ccgtcatggt agcctatcgg cggaatatgc
    27481 cgcaaggtcg gcaggaaaaa ggagaagaaa tggacaaaat cagagttgcc gccgtgcaga
    27541 tggtgtcggg cgtgtcgccg gaaaccaacg tcgctgccat gaaacgcctg gtcgcacggg
    27601 cggcggagca gggggcggat tgggtgctgc tgcccgaata ttgggtgctg atgggcgcaa
    27661 acgataccga caaactcgcg cttgccgagc ctttgggcgg cggacgcttt cagacggcat
    27721 tgagcgaaac ggcgaaagaa tgcggcgtgg tgctgttcgg cggaaccgtg ccgctgcaaa
    27781 gccccgaggc gggcaaggtc atgaatactt tattggtgta cgggcgcgac ggcaaacgga
    27841 cggggctgta ccacaaaatg cacctcttcg gcttttccgg tttgggcgaa cgctatgccg
    27901 aagccgatac catcagcgca gggcgggcag tgccgcactt gtcggcagag ggcgtgccgg
    27961 tggcggcagg tatttgttac gatgtccgct ttcccgaatt tttccgccgc cagctgccgt
    28021 ttgacgtatt gatgcttccg gcggcattta cccatacgac gggcaaggcg cactgggagc
    28081 tgcttttgcg tacgcgtgcc gtcgaaaacc aatgttatgt tgtggcggcg gcacagggcg
    28141 gattgcacga aaacggcagg cgcacgttcg gacacagtat gattgtcgat ccgtggggcg
    28201 acgtgttgga cgtattgccc gaaggcgaag gcatcgttac ggcagacatt gatgccaacc
    28261 gcctgaacag cgtccgcaac cgcctgcccg ccctgaaata ccgggttttg gatgccgtct
    28321 gaaggttcag acggcatcgg tgccggggaa tcagcgggct gtaggatggg cttcagcctg
    28381 ccatttccat caatcccaca tttctaccgt tttcatcgaa tccatcgaat ccgccccttt
    28441 cgacgaccca gtcctacgca actgaa
//
LOCUS       DAWFAD010000007        26646 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWFAD010000007 DAWFAD010000000
VERSION     DAWFAD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26646
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.007"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1075..2523
                     /gene="gnd"
                     /locus_tag="WGO49_000220"
     CDS             1075..2523
                     /gene="gnd"
                     /locus_tag="WGO49_000220"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ0305384.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDEFLNG
                     AAKGTGIIGAYSLQDLVDKLEKPRKIMMMVRAGSVVDDFIEQLLPLLEKGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGMGVSGGEEGARHGPSIMPGGDERAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSYDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2586..3857
                     /gene="waaA"
                     /locus_tag="WGO49_000221"
     CDS             2586..3857
                     /gene="waaA"
                     /locus_tag="WGO49_000221"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ0305385.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRGERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGVLMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRSLIRPAVASLTGCLVQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGDRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTAFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKTLSYEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3898..4347
                     /locus_tag="WGO49_000222"
     CDS             3898..4347
                     /locus_tag="WGO49_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ0305386.1"
                     /translation="MSIYALAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLPILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHAVVFTHMLLIVFLAKAMFYISW"
     gene            4380..5354
                     /locus_tag="WGO49_000223"
     CDS             4380..5354
                     /locus_tag="WGO49_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0305387.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVRQFEWILYLFGAFLLYTG
                     IHMMKPEGDEEEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5423..5498
                     /locus_tag="WGO49_000224"
     tRNA            5423..5498
                     /locus_tag="WGO49_000224"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5456..5458,aa:Lys,seq:ttt)
     gene            5718..6971
                     /gene="murA"
                     /locus_tag="WGO49_000225"
     CDS             5718..6971
                     /gene="murA"
                     /locus_tag="WGO49_000225"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ0305388.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVERLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            7052..8230
                     /locus_tag="WGO49_000226"
     CDS             7052..8230
                     /locus_tag="WGO49_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ0305389.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKNVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFTADAEAV
                     VKDIADVAEDDMILDIGPKSAAELADLLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(8297..8545)
                     /locus_tag="WGO49_000227"
     CDS             complement(8297..8545)
                     /locus_tag="WGO49_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0305390.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8636..9553)
                     /gene="ftsX"
                     /locus_tag="WGO49_000228"
     CDS             complement(8636..9553)
                     /gene="ftsX"
                     /locus_tag="WGO49_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ0305391.1"
                     /translation="MSIIHYASLHIESARTALKELLRQPFGTLLTLLMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLARDKRIANVRFIGKEDG
                     LAELQSNLDQNLVSMLDGNPLPDAFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSTVRPLVDAIFKPYGLNIGWRFFYPEEIA
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9550..10200)
                     /gene="ftsE"
                     /locus_tag="WGO49_000229"
     CDS             complement(9550..10200)
                     /gene="ftsE"
                     /locus_tag="WGO49_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ0305392.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKVHFNNQDLGTLTDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(10394..10876)
                     /locus_tag="WGO49_000230"
     CDS             complement(10394..10876)
                     /locus_tag="WGO49_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0305393.1"
                     /translation="MKYLNLAAIALAATFAAHTASADELAGWKDGTAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            11000..11353
                     /gene="arsC"
                     /locus_tag="WGO49_000231"
     CDS             11000..11353
                     /gene="arsC"
                     /locus_tag="WGO49_000231"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ0305394.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPDLDNDALLRAIAEHPALLERPIVLANG
                     KAAVGRPLENIEAIL"
     gene            11350..12006
                     /locus_tag="WGO49_000232"
     CDS             11350..12006
                     /locus_tag="WGO49_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ0305395.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIDSVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            12122..13516
                     /locus_tag="WGO49_000233"
     CDS             12122..13516
                     /locus_tag="WGO49_000233"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ0305396.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWADL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGANLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITTNEKLAEMVKPRLA
                     LRDIHETSKPALEDVLALVKDRAQDLNALADECLYFYKKQVPAEADVAKHWDDEASAR
                     MLRFAERLEGLEDWNTEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13537..13932
                     /locus_tag="WGO49_000234"
     CDS             13537..13932
                     /locus_tag="WGO49_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305397.1"
                     /translation="MPSDVGIRLQTAFKRKLKMKKIFYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            13936..14427
                     /locus_tag="WGO49_000235"
     CDS             13936..14427
                     /locus_tag="WGO49_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0305398.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDSGILLGSATVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14550..17144)
                     /gene="mutS"
                     /locus_tag="WGO49_000236"
     CDS             complement(14550..17144)
                     /gene="mutS"
                     /locus_tag="WGO49_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ0305399.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRNRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLASLRDSLFELAQIDLSANGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAAVNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17588..18592
                     /gene="gap"
                     /locus_tag="WGO49_000237"
     CDS             17588..18592
                     /gene="gap"
                     /locus_tag="WGO49_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0305400.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPSGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(18662..18781)
                     /locus_tag="WGO49_000238"
                     /pseudo
     CDS             complement(18662..18781)
                     /locus_tag="WGO49_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(18850..19530)
                     /gene="tsaA"
                     /locus_tag="WGO49_000239"
     CDS             complement(18850..19530)
                     /gene="tsaA"
                     /locus_tag="WGO49_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ0305401.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLNRIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEIRFQIEENRATV
                     IDLSPTPL"
     gene            19645..20280
                     /locus_tag="WGO49_000240"
     CDS             19645..20280
                     /locus_tag="WGO49_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ0305402.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGNWQVIVNEAACRGIAPDTIETAWQEMSSAGAVILKNAEEIKKYINNQ"
     gene            20318..21286
                     /gene="waaC"
                     /locus_tag="WGO49_000241"
     CDS             20318..21286
                     /gene="waaC"
                     /locus_tag="WGO49_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ0305403.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEAFNFVLDSQGLIKSACF
                     AKMAKSPIYGLDKNSAREGLAALAYAKTYAVPKGKNAVWRNRELFAQVFGYAMPETQV
                     FGLTVPEVGRLKNLAQPYYAALHATSRDSKLWPMENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQTLMDCVAADKG"
     gene            21512..22261
                     /locus_tag="WGO49_000242"
     CDS             21512..22261
                     /locus_tag="WGO49_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ0305404.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIECGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            22272..23207
                     /locus_tag="WGO49_000243"
     CDS             22272..23207
                     /locus_tag="WGO49_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ0305405.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAVDYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEVLKN"
     gene            23270..23881
                     /locus_tag="WGO49_000244"
     CDS             23270..23881
                     /locus_tag="WGO49_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ0305406.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFTYPIRTLADVKKYLDLLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILERDLQLDCRLTVSLSLVNYEACVAAWKNTRQIEVNTEKPFE"
     gene            complement(23933..24250)
                     /locus_tag="WGO49_000245"
     CDS             complement(23933..24250)
                     /locus_tag="WGO49_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ0305407.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLSTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            24408..25679
                     /gene="purD"
                     /locus_tag="WGO49_000246"
     CDS             24408..25679
                     /gene="purD"
                     /locus_tag="WGO49_000246"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ0305408.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDIISGLDAANQIGKVFHAGTTAKEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            25713..26315
                     /locus_tag="WGO49_000247"
     CDS             25713..26315
                     /locus_tag="WGO49_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ0305409.1"
                     /translation="MTDVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(26387..>26646)
                     /locus_tag="WGO49_000248"
     CDS             complement(26387..>26646)
                     /locus_tag="WGO49_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0305410.1"
                     /translation="SLSSCDKLDVSGFIHYRINHSKEFADRQNHINGIENFWNQAKRV
                     LRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
BASE COUNT         6419 a         6124 c         7475 g         6628 t
ORIGIN      
        1 acgtaaaatc taaagaaacc gtgctgtaaa cggcagactg atgccgtcat tcccacgaaa
       61 gtgggaatcc agaacgtaaa atctaaagaa accgttttcc cgataagttt ccgtaccgac
      121 aaggctggat tcccgcctgc gcgggaatga cggcggagcg gtttctgttg ctcccgataa
      181 attcctaaaa cttaaatttt catcattccc tcaaaaacag aaaaccaaaa acagaaacct
      241 aaaatcccgt cattcccacg aaagtgggaa tctagaacgt aaaatctcaa gaaaccgttt
      301 tcccgataag tttccgtacc gacaaggctg gattcccgcc tgcgcgggaa tgacggcaga
      361 gcggtttctg ttttttccga taaatgccgc aatctcaaat cccgtcattc ccactttcgt
      421 gggaatgacg gaatgtaggt tcgtgggaat gacgcggtac aggtttccgt tacgtatccc
      481 catccgaaag tttatttata cacaagcaat ccaaagttat ctgactgaaa tattgtgaga
      541 tttacccgca aatctcattt tttccaccac ttaatacaca aattcggatg cgtactctgc
      601 caatatgcag aaaaaagata tttgtatcga cacattctca ttcctacaag ttccaaacac
      661 acttccctct caaatctcca caacattccc gttcttaaag gcatcggcag ccattcggta
      721 aagaaaagcg gaatgccatc tgcacaacaa gcaaactgtc cacaaaaact attcttcatg
      781 aaaaacaatc cacaggtatg cacaaaaggg aatccgcctt accgacaccc ttttaaaacg
      841 gaaatcccaa tgatttttaa aataaaaaaa agttacccac agaaaatttc tcctatcaat
      901 ataatcatcg tttttttatt taatttttat attttattta taagccttca gggaaacgca
      961 cggtaaagcg taaaggcatc ttttcaaaaa attaaaaagt taaggattgt taaacaagtg
     1021 taaatcaggt agagttgcgg tcgataaatt aaatctataa cggagcttcc tgcaatgaaa
     1081 ggcgatattg gcgtaatcgg tttggcggtt atggggcaaa acctgatttt gaatatgaac
     1141 gattgcggat ttaaggttgt cgcctacaac cgaacaatcg gtaaggtgga cgaattttta
     1201 aacggcgcgg caaaagggac gggcattatc ggcgcgtatt ccctgcaaga cttggtcgat
     1261 aagctggaaa aaccgagaaa aatcatgatg atggttcgcg caggttcggt ggttgacgac
     1321 tttatcgaac aactgcttcc gttgttggaa aaaggcgaca tcattatcga cggtggcaat
     1381 gccaattacc ccgacacgac acggcggaca cattaccttg ccgaaaaagg gattttgttt
     1441 gtcggcatgg gggtctccgg cggagaggaa ggtgcgcggc acggcccgtc tatcatgccg
     1501 ggcggagatg agcgcgcatg ggaagctgtt aaaccgattt tccaggcgat tgctgcgaaa
     1561 acccctcaag gtgagccgtg ttgcgattgg gtcggcaggg atggtgcggg acatttcgtc
     1621 aaaatggtgc ataacggcat cgaatacggc gatatgcagt tgatttgcga agcgtaccag
     1681 ttcatgaaag acggtttggg actgtcctac gacgaaatgc accgcgtgtt tgccgagtgg
     1741 aacaaaaccg agctggattc ttatctgatt gaaatcacag cggcaatttt gggctataag
     1801 gacgaaggcg gcgaaccgct ggtcgagaaa atcctcgata cggcagggca aaaaggcacg
     1861 ggcaaatgga cgggcatcaa tgctttggat ttgggtattc cgttgacgct gatttccgaa
     1921 gcggtattcg cccgctgcgt ttcgtcgttc aaagaacagc gcgtgcagac cggcaagctg
     1981 ttcgcccgaa ccgttacgcc cgtcgaaggc ggcaaacaag aatgggttga ggcgttgaga
     2041 caggccctgc ttgcatccaa aatcatttcc tacgcacaag gctttatgct gatccgcgaa
     2101 gcgggcgaaa gctacggctg ggatttggac tacggcaaca ctgcgctgct gtggcgcgag
     2161 gggtgcatca ttcgcagcgc attcttaagc aatatccgcg acgcgtatga aaacaatccc
     2221 gatttggtgt tcttgggtgc ggatccgtat ttcaaaaata ttttggaaaa ctgcctgccg
     2281 gcgtggcgca aggtggttgc taaggcggtc gaatgcggca tccctatgcc ttgcatggct
     2341 tcggcgatta cgttcttgga cggctacacg accgaacgtc tgcctgccaa tctcttgcag
     2401 gcacagcgcg actacttcgg cgcgcacact tatgaacgca ccgacaaacc gcgcggcgag
     2461 tttttccata ccaactggac gggcaagggc ggagataccg cttcgacaac ctacgatatt
     2521 tgattccgtt gccgaaatgc cgtccgaacc gttcggacgg catttgtttt gcaggaaata
     2581 gaaagatgtt ccaatggctt tatgatgtat tgtggctgct tgcgccgata tggatacggc
     2641 gttatttgga caaacgctcc ggaagtgccc cggcatatcg ggcgcatcgg ggcgagcgtt
     2701 tcggcaagcc gtatccgaat cccgttaccg gcgcggtttg gatacacgcc gtttcggtcg
     2761 gggaaacgcg tgccgcccaa cctttgatac gcgagttgcg gcagcgtttt cccgatgcgc
     2821 cgctgctgat gacgcagatg accccgacgg gacgggaaac cgcgcaagtt ctgtttcccg
     2881 atgcgcaatg ccgctatctt ccgtatgaca aaaaaacgtg ggtacggcag tttttgcgcg
     2941 aacaccgccc gatgttcggc gtattgatgg aaacggaaat ctggcccaac ctaatgaagg
     3001 aatgccggcg cgcgggtgtg ccgctgtttt tggcgaatgc gcggctgtcg gaaaaatcgt
     3061 tgaacggtta tctgaaagtc cgcagcctga tccgtcctgc tgtcgcttcg ctgacgggct
     3121 gtctggtaca gacggaggcg gatgcggcgc ggttggcgaa attgggcgcg gcatccgtgc
     3181 aggtgtgcgg caataccaaa tacgacctga tgccgtcgga acagatgaaa acgctggcgg
     3241 ggcagtttga aaaacgcatc ggagaccggc cggttgccgt gtgcggcagc acgcgcgttt
     3301 atcggggtga agacgaggcg gagaaactgc tggcggcgtg gcaacaatat cgcggcgatg
     3361 ccctgctggt cgtcgtgccg cgccatcccg agcattttca gacggcattt gaaacggcaa
     3421 aacgcttcgg gtttaaggtt cagcggcgca gcgacggttt gccggtcgaa cccgatacgc
     3481 aggtgtggat aggcgacagt atgggcgagc tgtatgcgta ttacctgtgc gccgatgttg
     3541 cttttgtcgg cggcagtctg gtcgattcgg gctgtcagaa catcatcgaa ccgctttcct
     3601 gcggcgttcc gacgatattc ggcttttcga cctacaattt ttccgaagcc tgccggcacg
     3661 ccttggcatc gggtgcggcg gttcaagtcg aatcggcgga cgcgtggcgg gaagccgttg
     3721 aaaaaacctt atcgtacgag gggggcggaa tgcagatgca ggcgcgcgtg gacggcttta
     3781 tcgcacaaca tcgcggagcg ggcgcgagaa tcgccgaggc ggtgcgggaa gcggtatgcg
     3841 gacatcgggg gcgatagtga tacaatccgc atccgagttt tccggttgga gtaccgtatg
     3901 agtatttacg cacttgcaca catcgtccat ctgtattgcg ccattgcctt tgtcggcggc
     3961 gtgttttttg aagtgctggt tttgtccgtc ctgcatacgg gacgggtgtc gtgcgaggcg
     4021 cggcgcgaag tggaaaaggc aatgtcttac cgcgccgtca gggtgatgcc gtttgtggtc
     4081 ggactgctgt tcgccagcgg cgtcgtgatg gcggcaaacc gctaccttcc tatattgggc
     4141 gaaccgtttg ccacttcctt cggtacgatg ctgacgctga aaatcctgtt ggcgttcagc
     4201 gtgttggcgc acttcgccat cgccgtcgtc aaaatggcgc gttccacact gacggtcggc
     4261 tggtcgaaat acatacacgc cgtcgtcttt acccatatgc tgctgattgt ctttttggca
     4321 aaagcgatgt tttatatcag ctggtaacac aactacattt caaatcagga gattgaacca
     4381 tgacagaata tcccggcatc gggtcgccgt tgttttacgg cgtttttttt gcggcagtgc
     4441 tggtcatgat tgccttggat atgttttcgc tgaagaaaaa cggcagccat aaagtcggcg
     4501 tcaaagaagc cttggcatgg agcggcttgt gggttgccgt atcctgcctg ttcgcgggct
     4561 ggctgtattt cgaactcgcc ggcaatctcg gctacggcgc ggcagtcgca aaagaaaaag
     4621 tattggaatt ctttaccggc tacattttgg aaaaatcgtt ggcagtcgac aatattttcg
     4681 tgttcctgat gatattcggc tacttcaaag tcgcaccaca gtttcagcac cgcgtgctgc
     4741 tgtacggcgt attgggcgca ttggtattgc gcactgtcat gattttcgtc ggcgcggcac
     4801 tggttcggca gttcgagtgg attctgtatc tgttcggcgc gttcctgctc tataccggca
     4861 tacacatgat gaagcccgaa ggcgatgagg aagaggattt ggcaaacagc aggctgctga
     4921 atgccgtcaa gaaagtcgtt ccggtcggca cggaatttca cggcgagaaa ttttttaccg
     4981 tcgaaaacgg caaaaaaatc gccacgccgc tgtttttggt gctagtcatg attgaattga
     5041 gcgatgtcgt gtttgccgtg gacagcatcc ccgccgtctt tgccgttacc accgatccgt
     5101 ttatcgtgct gacctccaat attttcgcca ttttgggttt gcgggcgatg tatttcctgc
     5161 tggcggatgt ggcggaacgc tttatcttcc tgaaatacgg cttggcattc gtgttgggtt
     5221 ttatcggtgt gaaaatgctg gtaatgcatt gggtgcatat cccgatttcc gtttcactgt
     5281 cggtcgtgtt cggcgcgttg ggtgcatcga tactgacctc gttaatttat acgaaaaaac
     5341 agcctgataa ataaaatgcc gtctcaacgc cgacaaaatg gttgacagca tcaggcttga
     5401 tgtatagaat ttcgagcttg tcgggtcgtt agctcagtcg gtagagcagc ggacttttaa
     5461 tccgttggtc gagcgttcga atcgctcacg acccaccaga taaaacaaaa aaagctgagt
     5521 tttcaaaact cggctttttt tgttttccga caatccttgt tcggaaagcg cggcatttcg
     5581 tcaaattctt tggtgcggct tccgtatttt tcccgaaaat cccttataat tccgcctctg
     5641 caaccttcat cgggtgatgc tttatgcgtt gtcccgagaa ttgaaatatt acaagtaccc
     5701 accaggaaag aacaaacgtg gacaaactga aaatctccgc aaacggccca ctcaacgggg
     5761 aaataacggt ctcgggcgcg aaaaacgcgg cattgccgct gatgtgcgcg ggtttgctga
     5821 catcgggtac gttgcgcctg aaaaacgtcc ctatgctggc agatgtgaaa accacgcaaa
     5881 agctgcttca ggggatgggc gcgcgcgtcc tgaccgacaa tatcagcgaa tttgaaatta
     5941 acggcggcac ggtaaacaat acctgcgccc cttacgaatt ggtacggacg atgcgcgcct
     6001 cgattttggt gctgggcccg acgctggcgc gtttcggcga ggcgcaagtc agcctgccgg
     6061 gcggctgcgc catcggttcg cgccccgtcg atcagcattt gaagggcttg gaagcgatgg
     6121 gggcggaaat cgtcatcgaa cacggttacg tcaaggccaa aggcaaactc aaaggtacgc
     6181 gcgtggcgat ggatgtcgtt accgtcggcg gcacggaaaa cctgctgatg gcggcgactc
     6241 tggcggaagg tacgacggtt ttggaaaact gcgccatcga gcccgaggtg gtcgatttgg
     6301 cggaatgcct ggttaaaatg ggcgcgaaaa tcagcggcat cggaacatcg accatgatcg
     6361 tggaaggtgt ggacgagctg cacggctgcg aacacagcgt cgtccccgac cggatcgagg
     6421 cggggacgtt cctgtgcgcg gtggcgataa ccggtggcag ggtggttttg cggaatgccg
     6481 cgccgaaaac gatggaagtg gtgttggaca aactggttga ggcaggtgcg gtgattgagg
     6541 cgggcgacga ttggatcgcc atcgatatgc ggcagcgtcc gaaggcggtg gacatccgca
     6601 cggtcgtcca ccccggcttc cccacggata tgcaggcgca gtttatggca ttgaatgccg
     6661 tggcggaggg aagctgccgc gtggtggaaa cgatttttga aaaccgcttt atgcacgtcc
     6721 ccgagttgaa ccggatgggg gcgaacatta caaccgaggg caatacggca tttgtgcagg
     6781 gtgtggaacg gctttccggc gcagtcgtca aggcgacgga tttgcgtgcg tccgcaagcc
     6841 tcgttatcgc cggtttggcg gcgcgcggcg aaaccgtggt cgaacagatt taccacttgg
     6901 atcgcggtta tgaaaatatt gaaaaaaaac tcggcagcgt cggcgcgaaa atcgagcgcg
     6961 tatccggctg atcgggttgc gatgccgtct gaagccttca gacggcatca attgtttctg
     7021 tatcgcaaac actgattgaa aaggactgat tatggcattt ttaaaactga ccgaacaaaa
     7081 cgtgcagggc aaaaccgtcc tcatccgcgc cgatatgaac gtgccgttca aagacggcaa
     7141 aatcagcgac gacacccgta tccgcgcctc gctcgcgtcc atccaatact gcatggacaa
     7201 cggcgcatcc gttatcgtga tgacccactt gggccgcccg accgaaggcg agttccaccc
     7261 cgaagacgac gttgcgcccg ttgccgcgca tttgggcggc ctgttgggca aaaacgtgaa
     7321 agtattgaac gactggcgcg aaaacaaacc ggctttgaac gcgggcgatg tcgtgatgct
     7381 gcaaaacgtg cgtatcaaca aaggcgagaa gaaaaacgat ttggaactgg gcaaagccta
     7441 cgcttctttg tgcgacgtgt tcgtcaatga cgcgttcggc accgcgcacc gcgcccaagc
     7501 ctcgaccgaa gccgtcgccc aagccgctcc cgttgcctgc gccggcgtat tgatggcggg
     7561 cgaactcgac gctttgggca aagccttgaa acagcccgcg cgcccgatgg ttgccatcgt
     7621 tgccggcagc aaagtgtcca ccaagctgac catcctcgaa tcgctggcgg acaaagtcga
     7681 ccaactcatc gtcggcggcg gcattgccaa caccttcctg ttggcggaag gcaaagccat
     7741 cggcaaatct ttggcggaac acgatttggt ggaagaatcc aaaaaaatta tggcaaaaat
     7801 ggcggccaaa ggcggttctg tgccgctgcc gaccgatgtc gtcgttgcca aagcctttac
     7861 cgccgatgcc gaagcggtcg tgaaagacat tgccgacgtt gccgaagacg atatgatttt
     7921 ggacatcggc ccgaaatccg ccgccgagct tgccgatttg ctcaaagctg ccggtacggt
     7981 tgtatggaac ggccctgtcg gcgtgtttga gttcgatcaa ttcgcaggcg gcacgaaagc
     8041 tcttgccgaa gccattgctc aaagcaaagc attctcgatt gcaggcggtg gcgacacgct
     8101 ggcggcgatt gctaaattcg gcgttaccga ccaaatcggc tatatttcta ccggcggagg
     8161 cgcgtttctt gagttcttgg aaggtaaaga gttgcctgcc gtagccgctt tggaaaaacg
     8221 cggcgcgtaa gcggtttgat ataaaaaatg ccgtctgaaa cgttgtgctg tttctgacgg
     8281 catttttgtg tggcgattat tgtgctttgg ctgcccattc cgccggagtg gcggcaaccg
     8341 aaatggaaag tgcgtgcagt tcgttacttt ccagttgggc tttgagtccg tctttaatca
     8401 ggcggtggcg cgcgaggcgt gccttgcctt caaattctga tgaaacgatg acggcgaaaa
     8461 aatggtgtcc gtcgccttct acttcgatat gttcgcattt tgccacgcct tcaatcatgg
     8521 cttttacttg ttccgaggtc agcatggtag gttccttgtt ggaaaaatgc gccattatac
     8581 tggcaaattg aaatgccgtc tgaaaacggg ttcggacggc atttttgacg gtgttttatt
     8641 ttttggcttt gaagccgagc aggtgctggg tggtggcaag ccacgcgccg aatacgccca
     8701 acgcgatgac gaagccgaac accagcgcga tttcttcagg ataaaagaaa cgccagccga
     8761 tattgagccc gtagggtttg aagatggcat cgaccaaggg gcgcactgtg gaaagcagcc
     8821 aaccgcaaag ccccaggctg acggcggcgg aaaggatgct ctgccacatt gcctgataaa
     8881 ggaaggggcg gcggatgaac gatgcgggcg cgcccaagag tttggtgatt tcgatttctt
     8941 ctttgcggct gaggatttgc aggcggatgg tgttgtgtgc gacaaggacg aacgccatac
     9001 ccagcgtcag ggaaagaaac cataaaattt tacggataaa ttcgttgatt tggtacagcg
     9061 tttgcaccca ttcggtatcc atagacgcgg attcgaccat aggcagtttg gtaatgtccc
     9121 ggtagattgc ctgcatttgc gcgggcgttg tcgcggggtc gggggtaacg ataaatgcgt
     9181 cgggcagcgg gttgccgtcg agcatcgaaa ccaaattttg gtcgaggttg gactgtaatt
     9241 ccgccaagcc gtcttccttg ccgatgaagc ggacgttggc gatccgtttg tcgcgcgcca
     9301 gcaggctgcg gacggtatcg ctgtcgcttt gtgcggcggc ggtttccata tagacggtga
     9361 tttgcggcga ctcgttgagt ttgcccaaca cgctttgccc gctttgaatg cccaaataca
     9421 taaacagcgg cagggtcatc gcgacggcga gcatcaggag ggtgagcagc gtgccgaagg
     9481 gttggcgcag aagctctttg agcgcggtgc gggcggattc gatgtgcagc gaggcgtagt
     9541 ggatgatgct catgcgagtc gtcctttcga gaggcgcagg atgcggtgtc cgtagtccgc
     9601 catcagggtt tcgtcatggg cggcaacgat gacggttgtt cccgcttcgt ggaaggtttt
     9661 gaacaattcc atgatgtcga gcgcgtaggc gcggtcgagg ttggcggagg gttcgtcggc
     9721 aatcagcagg ccgggctggt gaacgacggc gcgggcgatg cacaggcgtt gttgttcacc
     9781 gccggagagg gttacgggat cgtccaattc tcgtcctttc aggccgactt tttcgatggc
     9841 gatgcgggcg cgttcttcgg ctttgcgcgg cggatagccg ataatccgaa gcggcaggat
     9901 gacgttttgc aggacgttgc ggtcgtagag gattttgtgg tcttggaaca cgatgccgat
     9961 gtgttggcgc ataaagccga tttggttgtc ggtcagtgtg ccgaggtctt ggttgttgaa
    10021 gtggactttg cccctgcttg gtttggtaat gcccgaaatc agcttgagga cggtggattt
    10081 gcctgaaccg gagtgtcctg cgataaaaat catctcgcct ttgttgattt ggaagctgac
    10141 gtttttcagg gcttcaaaac cgccgggata ggttttggaa acttgttcga aacggatcat
    10201 aggaggtcct gtaaggaagg gcggcatatc gtttcgggcg gttgcgtccg cagggatatg
    10261 ccgtctgaac aataggtaag acagtgccga ttataaagat tccgcctgct tttagatagt
    10321 ggataagttt ggcggatatg aaggtcgggc gggaaaaatg ccgtctgaag cggcttcaga
    10381 cggcatccgg cgtttaacgg cattttgaat gggcgagttt gacggcttcg gtcaggcttt
    10441 tttcgttcac ttccccggta atggtctgcc tgtatccgca tttcggtgct tcgacgacgg
    10501 taaagggcag tacgccgaca gtgtttccgt aggttttcat aaagtttcgg ctgttcgccc
    10561 cggtgtaacg ccaaatcggg taggaaacag gagtttgttt gaggaagttg ccgatattgt
    10621 cggatgtgtc gagcgcgatg ccgaccatat cgacgctgcc ttttttctgc gctttgtacc
    10681 atttggacat ggcgggcatc tcttttcggc acgggccgca ccaagtcgcc cacaggttga
    10741 cgatgcgtac gggggctttg agcgattgca ggctttgcgc ggtaccgtct ttccatccgg
    10801 caagttcgtc tgccgaggcg gtatgtgcgg caaatgtggc ggcaagggcg attgcggcaa
    10861 gattcaagta tttcatcgtt ttgtcctttg tatgttgatt cgggtattgt aaaccgtttg
    10921 gtacggttgg gggcgggata ttgagtaaaa ccctgcgtaa gccgataata aggggattgt
    10981 tgagaagcaa ggaaatgtca tgcctgaaat caaaattttc cacaatccgc gttgcagcaa
    11041 atcgcgtgcc gccttgtccc tattggaaga acgcggcatt gctgccgaag tggtcaaata
    11101 tttggatacg ccgcccgact tgtccgaatt gaaggatatt ttcaacaaat tgggcttggc
    11161 atcggcgcgc gggatgatgc gtgtgaaaga tgatttgtac aaggaattgg gtttggacaa
    11221 ccccgatttg gacaatgacg cgctgctgcg tgccatcgcc gagcatcccg ccctgttgga
    11281 gcgtccgatt gttttggcga acggtaaggc ggctgtcggc aggcctctgg aaaatatcga
    11341 agcgatattg tgaacaaacg gctgttttgc agccgcaacg gcttgcgcta ttacctgttg
    11401 ggcggtttct gtttgagtgt ttttccgctt ttgctggtgt ttgcctcatc cgtttgggcg
    11461 gtttatcgga cgggcgggca ggttttgccg ccgtatgtcc gggcggatgc cgcgctggtg
    11521 ttgggcgccg ccgcgtggga caaacgccct tccccggttt tccgcgaacg catcaaccac
    11581 gccatcgcgc tttatcagag ccgcagggta ggcaaaatcg tgtttacggg agggcgaacc
    11641 aaaaaaggct atatgaccga agccgaggtc gggcggcggt acgcgctcaa acagggggtt
    11701 ccggcgcgca acatcctgtt tgaaaacact tcgcgcaaca cttatgaaaa cctgaacaac
    11761 atccgtccgg ttttgcgtgc caacggtatt gacagcgtgg tgattgtcag cgatccctac
    11821 catttggcgc gggcggcgga aatggcggag gatttgggtg tccgcgttta tatgtctgcc
    11881 accccgacca cgcggtttga tgcgggaaat aagaaaaaaa tctttatgct gcaagaggga
    11941 tatgcgttat ccctctaccg tttggaaaaa tggggcagcc gctttttggg atggctgtct
    12001 gattgaagcg gtagaaaatg ccgtctgaaa cttatccgca ggcagggttc ggacggcatt
    12061 tttgatgccc aagcacggta aaaccagtaa aataaacgct ttcaaagatt tcggagccat
    12121 catgaccgtc aaaacccgtt tcgcccccag ccccaccggc tacctgcaca ttggcggcgt
    12181 tcgcaccgcc ttgttttcgt gggcgtttgc ccgccatcat aaaggcgaat ttttattgcg
    12241 catcgaagac accgacttgg cgcgttctac cgccgaatcc gtcaacatca tcctcgacgg
    12301 catgaaatgg gtcggcctga actacgacaa cgccgacaat gtcgtgtacc aaacccgccg
    12361 tttcgaccgc tacaaagaag tcattgccga acttttagaa aaaggccatg cctactactg
    12421 ctattgcagc aaagaagagc tggaagccat gcgcgagaaa gccgaaaaag aaggcacggc
    12481 gacttacgac cgccgctggc ggccggaagc gggcaaaacc ctgcccgaaa tccctgccgg
    12541 cgtgcaaccc gtcgtccgct tcaaaacgcc tttggatggc gtaaccaagt gggcggactt
    12601 ggtcaaaggc gaaatttcca tccccaacga agccctcgac gacctgatta tcgcacgcgc
    12661 cgacggcaca ccgacctaca acttctgcgt cgtggtggac gactacgaca tgggcgttac
    12721 ccacgttatc cggggcgacg accatgtgaa caacaccccg aaacaaatca acatcttaaa
    12781 agccatcggc gcaaacctgc ccgaatacgg acacctgccc atgattctca acgaacaagg
    12841 caaaaaaatc tccaaacgca gcggcgatac cgtcgccatt accgatttcg gcgcaatggg
    12901 catcctgccc gaagccatgc tcaactatct ggcgcgcttg ggctgggcgc acggcgacga
    12961 cgagttcttt acgatggaac aattcatcga atggtttgat ttgaaagacg tttccccgtc
    13021 tccaagccgt atggacttga aaaaactcta ctggatcaac ggcgaacaca tcaaaatcac
    13081 caccaacgaa aaactggccg aaatggtgaa accgcgcctc gccctgcgcg atatccatga
    13141 aacaagcaag cctgctttgg aagacgtgtt ggcattggtc aaagaccgcg cccaagattt
    13201 gaacgccttg gcagacgaat gcctgtattt ctacaaaaaa caagtccctg ccgaagccga
    13261 tgttgccaaa cactgggacg acgaagcctc cgcccgtatg ctgcgctttg ccgaacgcct
    13321 cgagggtttg gaagactgga ataccgaagc catccacgac ctgttcaaac ctttctgtga
    13381 cgaagaaggt attaaaatgg gcaaactcgg catgcctttg cgcctcgctg tctgcggcac
    13441 ggcgaagacc ccaagcgtcg atgccgtgtt ggcattaatc ggcaaagaag aagtgttgaa
    13501 acgcatccgc gcttaagtta tccacaggcg tgggtgatgc cgtctgatgt tggaatacgg
    13561 cttcagacgg catttaaacg gaagttaaaa atgaaaaaaa tattttattt tctgatggtt
    13621 gttttttcta caagcgtatg ggccggggat gcggaagaca atctgctcag catccaatcc
    13681 ggttatcgtg ccttactgca aaaacaaaac aatctggacg gaaaaatcat cgggatgcag
    13741 tcggatttgg aagatgcgcg cagacggttg caggcggctc aggcagacat cgcccgtttg
    13801 gaagcggaaa ttcctgcagc aatggcgcaa aaagcccggc aggctgaaga gctgaggcaa
    13861 atcggagtgc gtttggacca tgcttggaat gcggtttacg gcgcaggggg aacgaaagcg
    13921 tcggggaatt gaccgatgaa ttccctcttt gtggacaata ctgttttcat tacacggctg
    13981 aaagccgggc atatcggcag gttggttcag gcgttgtttg aggagtggca cggatttgaa
    14041 ccgtggtctt ctgtggataa gattcatgcc tattacggca ggtgtttgaa ggatgacgaa
    14101 ctgccgctgg catttgcggc tgtggatgat tccggaatcc tgttgggttc ggctacggtc
    14161 aagcggcatg atatggaaag ttttccacag tatgaatatt ggttgggtga tgtctttgtt
    14221 ttacctgaat atcgcggaaa aggcattggc aggaggctgg tcgcccattg cataggcgca
    14281 gcgcgttcgc tggggataaa gttcttgtat ctttatacgc ctgatgtgca aatattttat
    14341 gaatcattcg gctgggcggt tgtcgggcga catttccata acggtgaatg ggttacggtt
    14401 atgcgtttgg atatggataa ggtttaagcc gaatggacaa atgccgtctg agctgttcgg
    14461 acggcatttt tatgtttgga aacttcttgt cagataatgt ttatccacag atttttccat
    14521 atggtttgat gctgttccgc caacggaaat taagatacgg atttgcacaa atctttcaga
    14581 cggtacagtt ctgacaatgc ttcgcgcggg gtcaggctgt cgggatcgag tttttccaag
    14641 gctgctgcca atataccttc aaaatgtttt tcctctgctt tgtccacaaa gttgcccaca
    14701 ttcggttcat ctcctttttc agacggcatg gtactgaaaa tatccagttg gggacggttt
    14761 gcggcggctt ggttttccag tccgttcaaa tgcttttggg cggatttcaa tgcgcgtaca
    14821 ggcaggccgg cgagtttggc aacggcgatg ccgtagcttt tgccggcggg acccggttgg
    14881 atttggtgca ggaaaacgat gtcctgtccc tgttcgagcg cggaaaggtg catattgacg
    14941 gcggcggcgt gggcttcggg caggtaggtc agctcgaaat agtgggtagc aaacaggctg
    15001 aaggatttgt ttttttgcag caggtgttcg gcaacggcgt gcgcgagggc gaggccgtcg
    15061 aaagtggaag taccacgtcc gacttcgtcc attaaaacaa ggctttgttc ggtggcgtga
    15121 tgcaggatgt aggcggtttc gctcatttcg accatgaaag tggagcggtt ggaggcgagg
    15181 tcgtccgatg cgccgatgcg ggtgaagatt tgatcgatgg gcccgattgt ggcggcatcg
    15241 gcaggcacaa aacagccggt gtgtgccaat aaaacaatca gcgcgacttg gcgcatgtag
    15301 gtggatttgc cgcccatatt ggggccggtg agcagcatga ggcggtgttt gtggtcaagg
    15361 tcggtgtggt tggcggtgaa gtggcgtacc tgctgttcga caacgggatg gcggccgttt
    15421 tcgatgtgga taaccggata gtcggcaaac tcggggcgga cgaagttccg ctcttttgcc
    15481 aaggctgaaa atgtggacaa cacgtccagc gcggcggcgg ctttggcggc tttttgaagc
    15541 tgcggcaatg ccgtctgaag gtttttcaat acgccgtcaa agagttgttt ttctaaggcg
    15601 agggcttgct cttgagcagt cagcactttg tcttcaaagg ctttcaactc cggcgtgatg
    15661 aagcgttcgg cgtttttgag ggtttgccgg cgttggtagt cggcaggtgc ttgttcggct
    15721 tgggttttgg acaattcaat gtaaaagccg tgaacgcggt tgaactcgac tttgagtgtg
    15781 gacaaaccgg tacgttcgcg ttccttggct tccaaatcca gcaaaaattc gtcgccatgg
    15841 ttttgaatgc ggcgcaattc gtccagttcg ggatgaaaac cgtggttgat gacattgccg
    15901 tctttcagcc agacggaagg ttcgggcaaa atggcttggc ggagctgttc ggctgtggat
    15961 aggttttcag ggaaaacggc tttgagggtt tccaacaggc tgctgccgtt tgcggacaag
    16021 tcgatttgtg ccaactcgaa cagactgtcg cgtaaagatg ccaaatcgcg cgggcgggcg
    16081 ttgccgacgg cgatgcgggc agcaatgcgt tcaatgtccg caatgttttt cagacggcat
    16141 tggaggggtt tgtattggct ttccagcgcg gcaacggctt cttggcgcgc tcggatgtgg
    16201 gcgcggttgc gtaaagggtg gtgcagccag agagccaaga ggcggctgcc catatgggta
    16261 gcgcaaaggt cgagcgtgga catcagggtc ggcgattttt tgccggagag ggtttgcgtg
    16321 atttcgagat tgcggcgcgt ggcggcatcc ataccgatat attggctgtc ggtttcgagc
    16381 gacaggccgt ccaaatgttg cggcatcagg ttttgcgtca gacggatata gttcaacagt
    16441 gcacctgccg cgccaatcgc aacggcgtgt tctttgccgt ccaaaccgaa gccgcgcaaa
    16501 tcctggcagc cgaaatattc cgtcagcagt ttttcccccg cgtcggcggc aaactgccac
    16561 gcgttcaggc gcgtaacacc cgatgccgtc tgaagttgcg gtgcgttttt actgtcaggc
    16621 aacagaattt ccgccgcctg caggcgcgcc agttcgtcgt ccaatttatc cacagttgtc
    16681 agcttggttt tgaattcgcc gctttgcagc gatgcccacg ccaaaccgat gtattttttg
    16741 tcgggggaca cggcaacgat gcggttggtt tccttgtctt ccagcaatgc ggaatcggtc
    16801 agcgtgccgg gcgttacgat gcgcacgact ttgcgctcca ccggtccttt gcctgcgccg
    16861 acttcgccga cctgttcgca aatcgccacg cttttgccca acttgaccag gcgcgccaga
    16921 tattgttcgg cggcgtgaaa cggcacgcct gccattttga ccggctcgcc atccacctgt
    16981 ccgcgcgtgg tcagggtaat atccaaaagt tttgccgctt ctaccgcatc gtcgaaaaac
    17041 atctcgtaaa aatcgcccat acggtaaaac accagtttgt cggtatgttg cgctttgatg
    17101 ccgaggtatt gctgcatcat tggggaaacg gcggatttgg acatggggct gccttatcat
    17161 taggaaaacg gtattgtagc aaaaggtttt gtcttcgacg gaatgagact gaccgtaagc
    17221 ctgtcaataa ggttgtggag acaactagga aaacagcaaa gttatccaca gaaatattat
    17281 taagtcaaaa aacatccacg cttatctgtc ttatacagca agtcttaaac tcaaacttat
    17341 ttgacaaaaa ggtaatgaaa atattggata tttgctactt acgcacaagt aggttggcaa
    17401 tcatcaaaat catcattaaa attttaaatt caaatattga tggtaaaagt tttggaaatc
    17461 tgtgcaaaca gtaaaattca tctatatcaa ataagcatca agtagttaga tgtaatagtg
    17521 gaatgtaact ttatttcaga atacaataca ggtattgaaa tttcgtttca cagcttggag
    17581 tatatacatg agcattaaag tagcgattaa cggtttcggc cgcatcggcc gcctcgcatt
    17641 gcgtcaaatt gaaaaagccc acgacatcga agtcgtcgcc gtcaacgacc tgacccctgc
    17701 cgaaatgctg ctgcaccttt tcaaatacga cagcacgcaa ggccgtttcc aaggtactgc
    17761 cgaattaaaa gacgatgcca tcgtggtaaa cggcaaagaa atcaaagttt tcgccaatcc
    17821 gaatcccgaa gaattgcctt ggggcgagtt gggtgtggat gtcgtcctcg aatgtaccgg
    17881 cttctttacc aataaaacca aagccgaagc ccacatccgt gccggcgcgc gcaaagtcgt
    17941 gatttccgca cctagcggca atgatgtgaa aaccgtcgta tatggcgtaa accaagacat
    18001 tttggacggc agcgaaaccg ttatctccgc tgcttcctgc accaccaact gccttgcccc
    18061 gatggcggca gtcctgcaaa aggaattcgg cgtagtcgaa ggcctgatga ccaccatcca
    18121 cgcctacacc ggcgaccaaa acacccttga cgcgccgcac cgcaaaggcg atttgcgccg
    18181 cgcccgcgcc gccgcgctca acatcgtgcc caacagcacc ggtgccgcca aggccatcgg
    18241 tctggttatc cctgaattga acggcaaact cgacggctcc gcccaacgcg tccctgttgc
    18301 caccggctcg ctgaccgaat tggtttccgt cctcgaacgc cctgtaacca aagaagaaat
    18361 caacgccgcg atgaaagccg ccgccagcga atcttacggc tacaacgaag atcaaattgt
    18421 ttcttccgac gttgtcggca tcgaatacgg ttcgctcttc gatgcgaccc aaacccgcgt
    18481 gatgaccgtg ggcggcaaac aattggtgaa aactgttgcc tggtacgaca acgaaatgtc
    18541 ctacacctgc caactcgtcc gcactttgga atacttcgca ggcaaaatct aaggcttgaa
    18601 caaacctgtg gataagtaaa tgccgtctga acacattgcg ttcagacggc attgtttatc
    18661 tgtatagtgg attaaattta aaccagtacg gcgttacctt gccttgccgt actatttgta
    18721 ctgtctgcgg cttcgttgcc ttgtcctgat ttaaattcaa tccgctataa cttgatattg
    18781 cggcttatgg cgaacaaaca acagccgttt gttcaactgc tatgcggcaa aaccggattt
    18841 ttgcccgatt taaagcgggg ttggggaaag atcaataacg gttgcacggt tttcctcgat
    18901 ttgaaatctg atttcgtaat ccgcaatatt catcacataa atccgttcgg gaatattctg
    18961 ataggcgggg cgcggatctt gggcaatgct ttggctgata aggtttttgg tgtttgcaga
    19021 taaattttcc gcgccgatgt tttcctgcca aacgacttcc aactctacgg gtttgccgct
    19081 gacgaaacca gatgcggcat cgggtttgga ttcgacaaag gggatataag gcttgatgtc
    19141 cacaatcggc gtgccgtcga gcaggtctgc gccgctgcaa taaaggcgga cgggtttgcc
    19201 ggtttcgatg cggttgagtt ttaagagtga tagtccgaga tggttggggc ggtgggggct
    19261 gcgcgtggcg aatacgccca ttttttgttt gccgccgagc cgtggtgggc gcaccatttg
    19321 tgcccagcct tcatccaata cgccgtgaaa aataaaactt atccacacat aatcgaaatc
    19381 ttccagcccg cgcacgctgt ctgcggtaaa ttcgggattc agctcgatgc atacttctgc
    19441 ggcggagact aaaccgggct ggcgggcgat gccgaatttc tgtttgtagg gcgatcgggc
    19501 ggtgccgatg ggggtaatgg tataagtcat aaggttgtgg ataagttttt ttggaaaaac
    19561 cttatcttat cacaatcggt tttaccgttt ttgggcagta atttgttata atacagggca
    19621 ttttaatcgc gttgaaataa tgagatgatt gtttccattg atgttgatgc acaaaaaacg
    19681 tttacgccac tgtgtcctga cgaactgccc gtgaacgagg ggcatttgat tgtcgaggag
    19741 ttgaatgctc aagccgcttt ggcggatttg cgcgtgatga cgaaagatgc gcatcatatg
    19801 gtagcgaaat ggcttgtgga taaccctgtt gatatgttga agccgacagg tttgcctgat
    19861 gcggatttga cttgggtggc tcatgcgatg gtcggtacgc gcggctatga attattggac
    19921 ggactgcctt ctgctaaaga atatgattat tgcgtttgga aaggtgttga tcctgaattg
    19981 catccttacg gcgcgtgttt tcacgatatt gaggaaaaac taagcacagg gctgattgaa
    20041 tggctgcgtt gtcaaaatac ggatacggtt attatcggtg ggttagctac ggattattgt
    20101 gttaaaacaa cggttttgca gttacttaaa ggtggtaatt ggcaggttat cgtcaatgaa
    20161 gcggcttgtc ggggcattgc gccggatacc attgaaacgg catggcagga aatgagttct
    20221 gccggtgcag tcatcttaaa aaacgctgaa gaaattaaaa aatatattaa taatcaataa
    20281 gttaaaattg tttcacgtga aacatacttc ttaaaatatg aaaattttgc ttgtccgttt
    20341 gtctagtatg ggcgatttaa ttcacacttt gcccgcaatt gaggatttgg cgcgacaatg
    20401 ccctgatgta gaactccatt ggctgtgtga agctggattt gcagatattg cgcgcctgca
    20461 tccgtttgtg aaaaaaatcc atgtgatgaa atggcggcaa tggcgcaaac atctctttca
    20521 ggctgaaact tggcgggaaa tgggtcatct gaaacagact ttgcggcagg aagcatttaa
    20581 ttttgtattg gacagtcaag gtctgattaa aagcgcgtgt ttcgctaaaa tggcgaaatc
    20641 ccctatttat ggtttggata aaaatagcgc gcgcgaggga ctggctgcgc tggcgtatgc
    20701 gaaaacatac gctgtaccga aagggaaaaa tgctgtttgg cgcaatcgtg agctgttcgc
    20761 ccaagtattt gggtatgcga tgcctgaaac gcaggtattt ggcttgactg ttcctgaggt
    20821 aggccgtctg aaaaatttag cgcagccgta ttatgcggct ttgcatgcga ccagtcggga
    20881 cagtaagtta tggcctatgg aaaactggcg gtcgttgctg caaaagttga atgaagaaca
    20941 gcaatgcaat atttacctgc cttggggaaa tgaagatgaa aaaacgcgtg ccaaacagat
    21001 tgcagacgga ctgccgtttg ctattgtgtg cgacaaaata aatttattgc aggcagcgta
    21061 tctgctgaaa tacgcggtcg gaattgtcgg cgtggatacc ggtttgctgc atttggcaaa
    21121 tgccttggaa aaacctgtgg tcggcattta taccgatacc gatccgatta aaacaggcgt
    21181 tcaggtttcg ccaattgcaa aaaatttggg caatatcggg cagattccga ccgcagattt
    21241 ggtttatcaa acgttgatgg attgtgttgc agcagacaaa ggctaaaggg ttgtctgaaa
    21301 ctgatatagc acctgatatt tttcagtttg ttaagcataa gaatgtatat ctaccagcct
    21361 gagtaaatgc tttaaaaatg gtataatcgg caagcattat ccgaaagatt tttgaatgaa
    21421 ttcgttttta aggaaaacgg atttggtttt atagggaaga aggtttggtt tcgctggatg
    21481 gtgaaagcag cgttcttttt aggagggttc aatgaaagca ctggtcgcag taaagcgcgt
    21541 agtggactac aacgtcaaag ttcgtgtaaa agccgatggt tcggatgtgg atatcggcaa
    21601 tgtcaaaatg tcgatgaatc cgtttgacga aatcgctgtg gaagaagctg tccgtttgaa
    21661 agaagccgga aaagtaagcg aaatcgtagc ggtttctttg ggtgagaaaa aatgcgaaga
    21721 aaccttgcgt acagctttgg cgatgggtgc cgaccgtgcc attcatgttg aaaccgatac
    21781 taaactggag tctctggccg ttgctaagtt gctgaaagcc gttgcggaca aagaaaatcc
    21841 gcaaattttc tttttgggca aacaagcgat tgatgatgat gccaatcaag tggcgcaaat
    21901 gctggcagct ttgctgaatg cggcgcaagg tacgtttgct tcaaaagtac acattgaatg
    21961 tggtgaagta cagattgtgc gcgaaattga tggcggagaa gaaaccatag cattaaaact
    22021 gcctgctgtt atcagtgctg atttgcgttt gaacgagccg cgctttgtca aactccccaa
    22081 tattatggcg gcaaagaaaa aacctttgga aaaactgact cctgacgatt tggttgccga
    22141 catttcacct cgtttgaaaa cggtgaaatt tgccgaacct aaggcgcgtc aggcaggcgt
    22201 aaaagtagca agcgttgccg aattggttga aaaattgaaa aacgaagcca aagtgatttg
    22261 aggaggctga aatgagtgta ttgattattg tcgaacacga caacaaacag ttgaatccta
    22321 ccactttgca tgctgttacc gctgccgcta agctgggtaa aatcgattta ttggttgccg
    22381 gaagcggtgc atctgctgtg gtagagttcg caaaacaagt ggcgggtgtg gaaaaagttt
    22441 tggttgcaga tgctgcccat tatgccgaag gtttggctga agagttggct ccgctggttg
    22501 ttaaattggc agtggattac tgctatgttg cagcaacggc aactacattc ggtaaaaacc
    22561 ttttgcctcg cgtagcagcc ttattagacg taccgcaaat ttctgatttg accgaaattg
    22621 tggataacac gacttttgtg cgccccattt atgcgggtaa tgcatttgaa accgtgcaag
    22681 ctgattcaga aaaattggtg ctgaccttcc gtgcgacggt ttttgacgca gtggcggcgc
    22741 aaggtggtaa tgctgaggta attaatgttg aagcaacccc tgcccaaaac ctgagtcgtt
    22801 ttgttaaccg tcagctttcc cattccgatc gtcctgaact gactcaggca aaagtgattg
    22861 tttccggtgg tcgtgcgttg ggtagtgcgg aaaaattcaa tgaagtgctg acaccgttgg
    22921 cggatgtttt aggtgcggca atcggtgcat cccgtgcagc agttgatgcc gaatatgcgc
    22981 caaacgatgc tcaagtcgga caaaccggta aagtggttgc gccgcaactc tattttgcaa
    23041 tcggtatttc aggtgcaatt caacacgttg ccggtatgca agacagtaag gtgattgttg
    23101 caatcaataa agatgctgat gcgccgattt tcaatgtagc cgattacgga ttggttggcg
    23161 atttatttga aatcgttcca caattaacag aagtattgaa aaattgatgt ttcacgtgaa
    23221 actttcagac gaccttgtta ttaatgaggt cgtctgaaaa catttcagca tgattacgac
    23281 ttataaaacc attacttcct cgacgcaggc tgagtttaaa gataaaggca gccgttttat
    23341 tgcatttacc tatccgattc ggacattggc tgatgtgaaa aaatacctcg atctgttaaa
    23401 ggaagagcat cataaagcac gacactggtg ctatgcctat cgtttgggtg tggatggtgt
    23461 gcaatttcgt gccaacgatg atggagagcc atcaggaagt gctggacgac caattttggg
    23521 acagattgat tcggtgggta ttaccgacgt tttggttgtg gtcgtccgct atttcggtgg
    23581 tactttgtta ggtgttccag gtctgataca tgcgtacaaa gaggcgacgg ctcaagcgtt
    23641 ggcagttgca gaagtagttg aaaagaatat tgaaaaaact gtttggctga aatgtgaata
    23701 tccgtttttg aatgaagcga tacgcattgc caaacaatat caggcggata tattggagcg
    23761 ggatttgcag ttggattgcc gattgacggt cagtttgtcg ttggtaaatt atgaggcctg
    23821 tgttgcggct tggaagaata ctcggcagat tgaagtaaat acggaaaagc cttttgaatg
    23881 aaaggtcgtc tgaaatgctt tgtgatggta tttcagacga cctcttcgct gactaatctt
    23941 cttgtgtata tttgtctaag gcatcgacgc tggagcgcaa atataaccaa gccaattgat
    24001 ggaattcgcc cagcgcgttc catagcgcat cttcactcat aatgctgtat tcgccttccg
    24061 tactcaaatc cagttctacc ccgtcttcaa tagcctgatg acacgcctga tactcgtggg
    24121 tatagaaatt gtccatgtcg cgaattaaag tgacgacata tttccaacgg cggatgtctt
    24181 cttccagttg cggtagtaaa tgacaccatt cgcggtattt gctttggatt tcatcaatac
    24241 gtttttgcat ggttgcttgt ccttttttaa tttatggata agttgaatag gttgcagagt
    24301 ggaatgaagc ggtcttttgt ttgattctac taaaatttct tcagatggga taaaagtccc
    24361 tttgtatcga gttttcgtta aaagtacata tttgtcaagg agatggtatg aaactgctgg
    24421 ttatcggtaa tggcggtcgc gaacacgcgc tggcttggaa attggcgcag tcgcctaaag
    24481 tggaaacagt atttgttgcg cctggtaatg ccggtacagc gattgaaccc aagttgcaaa
    24541 acatcgactt gactgcgcat caggatttga ttgaattctg tcgtaaagaa aatattgttt
    24601 ttaccgtcgt cggccctgaa gcgcctttgg cggcaggtat tgtggatgat ttccgtgctg
    24661 caggactaaa aatattcggt ccgacacaat atgcggcgca gttggaaagt tccaaagatt
    24721 tcgccaaagc atttatggcg aaatacaata ttccgaccgc gcaatatcaa acctttgaaa
    24781 acgccgatgc cgcacatgat tacgtcaatc agaaaggtgc gcctatcgtt atcaaagccg
    24841 atggtttggc ggcaggtaaa ggcgtgattg tggcgatgac tttggatgaa gcgcatgctg
    24901 cgattgatga tatgctgctg gacaacaaaa tgggcaatgc cggcgcgcgt gttgtaattg
    24961 aagatttcct gcaaggcgaa gaagcgagtt ttatcgtcat ggttgatggc aataatgtac
    25021 tgcctatggc aaccagccaa gaccacaagc gtcttttaga cggcgacaaa ggtctgaata
    25081 cgggcggtat gggtgcgtac agccctgcgc ctgtggtaac gcctgttgtg tacgaacgcg
    25141 cgatgaatga aattattttg ccgaccgtag cagggatgaa agcggagggg catgagttca
    25201 ccggtttcct gtacgcaggt ttgatgattg atcaaagcgg tgcaccctat acgattgagt
    25261 ttaactgccg tttcggcgat cctgaaaccc agccgattat gagccgactg aacagcgact
    25321 tatcggattt ggttgaagcg gcaatagacg gcaaactcga tagcgtaacc gcggaatgga
    25381 gcccgcaaac tgccgtgggc gtggtactgg cggcgcaaaa ttaccccgaa acgcccaaaa
    25441 aaggcgatat tatttccggt ctggatgctg ccaaccaaat cggcaaagtt ttccatgcag
    25501 gtacaacggc aaaagagaaa ggtgacgtat tgaccaacgg cggacgcgta ttgtgtgttg
    25561 tgggattggg cgacaatgtg gcgcaagcga aagctaaggc atatggcgcg ttggaaaaaa
    25621 tcagtttcga cgggatgcaa taccgtaaag acattgccga taaagcgatt aaccgttaac
    25681 tagaaagagg atgatgccgt ctgaagccaa gtatgactga tgtgcttcag acggtatttt
    25741 atattatgtt atttccgaga attatcgcag catcagcgca acgtaagtta agcgtttatt
    25801 tgaaaaaagg cggtttggtc gcttatccga ccgaatcttg ttatggcttg gggtgtcttc
    25861 cgacattagc caaagcactc ggtaagttgg cgcatttgaa aaaaagaccg caacacaagg
    25921 gtatgattgt catcgggaat caatttgagc aattgcagcc tttacttcaa atgccgtctg
    25981 aaaatctgca ggatatgctt agaaaagaat ggcccgcccc taaaacgttc ctattatccg
    26041 ccaaatcctg tgtcttacct gagttgcgcg ggaaacagcg cagcaagttg gcggtcaggg
    26101 ttcccgccca tgtaggtgcg cgccgcttgt gtcaggcttt gcaaacgcct ttggtctcga
    26161 cttcgtgcaa ccgcgcaggc aaacgcgcgt gtcggacgga gagggaagtc agaaggcaat
    26221 tcggacgcga tgtgtggatt gttggtggga gaatcggcag gcagaaatcg ccgagtcaga
    26281 ttattgacgg ggaaacaggt aaacgtttgc gttagatatg ggtatttgaa tgaaaacttt
    26341 ttttgcatac acaaagaaaa aattttgggg ctgtactaga ttagccctaa atcccacacc
    26401 aatcccgcag gattttaagc tgttgagacg gtgtgccgaa gttaaatcga aattcgcatt
    26461 ctttcaagaa cagcgggaaa gatttgcgat cgattccatt gtattttcgc aagacgcgtt
    26521 ttgcctgatt ccaaaagttc tcaatgccgt taatgtggtt ttgacggtct gcaaactcct
    26581 tggaatggtt aatgcggtaa tggataaaac cgctcacgtc caacttgtcg cagctgctca
    26641 gactat
//
LOCUS       DAWFAD010000008        26524 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWFAD010000008 DAWFAD010000000
VERSION     DAWFAD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.008"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            123..2150
                     /gene="uvrB"
                     /locus_tag="WGO49_000249"
     CDS             123..2150
                     /gene="uvrB"
                     /locus_tag="WGO49_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ0305411.1"
                     /translation="MEVIRYPDSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IDFFTKENRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDGGKGRRQGKNKVKV
                     SEIHNEEDAVKEIAKLEKAMQQAARDLQFEEAAVLRDRIRGIKEGLLFGSE"
     gene            2262..2549
                     /locus_tag="WGO49_000250"
     CDS             2262..2549
                     /locus_tag="WGO49_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305412.1"
                     /translation="MTYSFRVENINDISDIPENHLEIVRAALPSNRSNALSREPDGAQ
                     AVFVYEIDGEFRAKLFDRIKDTDSYLQATEDNYSNDPRSIGVVYVLEQQQG"
     gene            complement(2730..3446)
                     /gene="trmB"
                     /locus_tag="WGO49_000251"
     CDS             complement(2730..3446)
                     /gene="trmB"
                     /locus_tag="WGO49_000251"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ0305413.1"
                     /translation="MTDTAENQTQNNWQAEHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVIENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYARQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRMG"
     gene            complement(3568..4632)
                     /locus_tag="WGO49_000252"
     CDS             complement(3568..4632)
                     /locus_tag="WGO49_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0305414.1"
                     /translation="MYLLYASRHFEEALEWAKTSAKNNNPHGQYLLAQYCRYGTPPDF
                     ETAHLLYRKAAAQGLPEAHWQLGLQYRFGQGTKVDTAQAVNHLRAAAQQGYIPAYTPL
                     AELILPTAPDEAVYRFQQAAQENDPDAHAALADIYLQGKYQERNHKLALHHAEAAAAE
                     RHPEGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLSAYQKLISDSALNHPDQYG
                     GIKDSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMY
                     YFGQGIAADYGKARKWFEQAAAQKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAIN
                     NGYGQKSVLQELLQQWQNAV"
     gene            complement(4697..4972)
                     /locus_tag="WGO49_000253"
     CDS             complement(4697..4972)
                     /locus_tag="WGO49_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305415.1"
                     /translation="MNNPDLPYRQTLECLSQKQYNFTEVRRLLTEAALAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPP"
     gene            complement(5089..6975)
                     /gene="uvrC"
                     /locus_tag="WGO49_000254"
     CDS             complement(5089..6975)
                     /gene="uvrC"
                     /locus_tag="WGO49_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ0305416.1"
                     /translation="MPSEAVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSESEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPDPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPHSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(7030..9192)
                     /locus_tag="WGO49_000255"
     CDS             complement(7030..9192)
                     /locus_tag="WGO49_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ0305417.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VVFDDSKTSVADIAKIIEKTGYGAKEKTEDTLPQAEAEHHIGWRLWLLSAINIPFLVG
                     MAGMMIGRHDWMIPPLWQFALASIVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAHGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRDGEWKQLPIDQVQIGDLIRANHGERIAADGVIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRAAQLGSQTLLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPAVVGIALLTFIVTWLIKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDKDDLYRI
                     AAAIEQNAAHPLARAIVSATQARGLEIPAAQNAQTVVGAGIAAEVEGAGLVKAGKAEF
                     AELTLPEFSDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAQQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPVIAGAAMAASSVSVLSNALRLKRVKID"
     gene            9331..10281
                     /gene="trxB"
                     /locus_tag="WGO49_000256"
     CDS             9331..10281
                     /gene="trxB"
                     /locus_tag="WGO49_000256"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ0305418.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            10436..10804
                     /gene="rpsF"
                     /locus_tag="WGO49_000257"
     CDS             10436..10804
                     /gene="rpsF"
                     /locus_tag="WGO49_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ0305419.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            10805..11107
                     /gene="priB"
                     /locus_tag="WGO49_000258"
     CDS             10805..11107
                     /gene="priB"
                     /locus_tag="WGO49_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ0305420.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHESWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            11114..11344
                     /locus_tag="WGO49_000259"
     CDS             11114..11344
                     /locus_tag="WGO49_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ0305421.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            11361..11813
                     /locus_tag="WGO49_000260"
     CDS             11361..11813
                     /locus_tag="WGO49_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ0305422.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEVRRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            11827..11934
                     /locus_tag="WGO49_000261"
     CDS             11827..11934
                     /locus_tag="WGO49_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ0305423.1"
                     /translation="MPSETLPIVGMWFQTVFVLFSVYSAKVGMVVFIVD"
     gene            complement(12601..13407)
                     /locus_tag="WGO49_000262"
     CDS             complement(12601..13407)
                     /locus_tag="WGO49_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0305424.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKRAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGRQTASRPLPKLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(13408..14154)
                     /gene="pssA"
                     /locus_tag="WGO49_000263"
     CDS             complement(13408..14154)
                     /gene="pssA"
                     /locus_tag="WGO49_000263"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ0305425.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            14737..15219
                     /locus_tag="WGO49_000264"
     CDS             14737..15219
                     /locus_tag="WGO49_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ0305426.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(15271..16482)
                     /locus_tag="WGO49_000265"
     CDS             complement(15271..16482)
                     /locus_tag="WGO49_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ0305427.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            16705..19143
                     /locus_tag="WGO49_000266"
     CDS             16705..19143
                     /locus_tag="WGO49_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ0305428.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPSPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDSPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            19352..20662
                     /locus_tag="WGO49_000267"
     CDS             19352..20662
                     /locus_tag="WGO49_000267"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ0305429.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NSDRVIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEEYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDIFKEQAERRVSLGLILAKLVDENKLEPTEEQVKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            20758..21372
                     /gene="clpP"
                     /locus_tag="WGO49_000268"
     CDS             20758..21372
                     /gene="clpP"
                     /locus_tag="WGO49_000268"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ0305430.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(21429..22694)
                     /gene="ispG"
                     /locus_tag="WGO49_000269"
                     /gene_synonym="gcpE"
     CDS             complement(21429..22694)
                     /gene="ispG"
                     /locus_tag="WGO49_000269"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ0305431.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKITLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(22710..23471)
                     /gene="pilW"
                     /locus_tag="WGO49_000270"
     CDS             complement(22710..23471)
                     /gene="pilW"
                     /locus_tag="WGO49_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ0305432.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLIGQ"
     gene            complement(23474..24568)
                     /gene="rlmN"
                     /locus_tag="WGO49_000271"
     CDS             complement(23474..24568)
                     /gene="rlmN"
                     /locus_tag="WGO49_000271"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ0305433.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(24711..25136)
                     /gene="ndk"
                     /locus_tag="WGO49_000272"
     CDS             complement(24711..25136)
                     /gene="ndk"
                     /locus_tag="WGO49_000272"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ0305434.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(25195..26346)
                     /locus_tag="WGO49_000273"
     CDS             complement(25195..26346)
                     /locus_tag="WGO49_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ0305435.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
BASE COUNT         6292 a         6628 c         7222 g         6382 t
ORIGIN      
        1 tgggtagcga ttgggtttca gacggcatac attcaggatt tcaccgcctt atgccgtctg
       61 aaagacgttt ccgacccatc cctactacac gctttgtccg caaacgcatc acaggcaaga
      121 ttatggaagt catccgatac cccgattccc ctttcaaact ccaccaaccc ttcccgcccg
      181 caggcgacca gcctaccgct attgccggct tgctcgaagg gctttcagac ggccttgcct
      241 accaaaccct gctcggcgta accggttcgg gcaaaaccta caccatggcg aacgtcatcg
      301 cccaaagcgg ccgacccgcc atcatcatgg cgcacaacaa aacccttgcc gcccagcttt
      361 atgccgaaat gcgcgagttt ttccctgaaa acgcggtgga atatttcgtc tcctactacg
      421 actattacca acccgaagcc tatgtgccca gccgcgacct gttcattgaa aaagacagcg
      481 cgatcaacga acatatcgag cagatgcgcc tttccgccac caagaacctg atgacgcgcg
      541 acgacgtgat tatcgtcgcc accgtgtccg ccatctacgg tatcggcgac ccgaccgagt
      601 atcagcaaat ggtgttgtcc gttaaagaag gcgacaccat tgagcagcgc gacatcatcg
      661 ccacgctcgt ttccatgcag tacgagcgcg gcgatttgga ctttaaacgc ggcagcttcc
      721 gcgtgcgtgg cgacgtgatt gacgtgtacc ccgccgaaag ctccgaaaac gccttgcgca
      781 tcagcctgtt tgacgacgaa atcgaccgcc tcgatatgtt cgacccgctt tcaggcagcc
      841 ttatccagcg cgtcggccgc tacaccgtct tcccgtccag ccattacgtt accccgcgcg
      901 ataccgtctt gcgcgcctgc gagtccatca aagaagaatt acgtgaacgc attgatttct
      961 ttaccaaaga aaaccgtccc gtcgaacaac aacgcatcga acagcgcacc cgctttgacc
     1021 tcgaaatgct ctacgaaatg ggcttctgca aaggcatcga aaattactcc cggcacttct
     1081 ccggcaaaaa agaaggcgaa ccgccgccca cgctgatgga ctacctgcct gacaacgcca
     1141 tcatgttcat cgacgaaagc cacgttaccg tcacccaaat cggcggcatg tacaaaggcg
     1201 acgcatcgcg caagcaaaac ctcgtcgact acggcttccg cctgccttcc gcccgcgaca
     1261 accgcccgct caaattccat gaatttgaaa aagtcatgcc gcaaactgtc ttcgtttccg
     1321 ccactccagc caaatacgaa gaagaacacg ccggacaagt ggtcgaacaa gtcgtccgcc
     1381 ccacagggct ggtcgacccc caaatcatca tccgccccgt cgccacccaa gtcgacgact
     1441 taatgagcga aatcaacgac cgcatccaaa aaggcgaacg cgttctcgtt accaccctca
     1501 ccaaacgcat ggcggagcaa ctcaccgact attacagcga actcggcatc aaagtgcgct
     1561 acctgcacag cgacattgat accgtagagc gcgttgaaat cattagagat ttacggctcg
     1621 gcctgtttga cgtactcgtc ggcatcaacc tcttgcgcga aggcctcgat atccccgaag
     1681 tctccctcgt cgccatcctc gacgccgaca aagaaggctt cctgcgctca caccgcagcc
     1741 tgattcaaac cataggccgc gccgcgcgca acgtgaacgg cgtcgcgatt ctgtacgccg
     1801 acaaaatcac cgactctatg aaagccgcca tcgacgaaac cgaacgccgc cgtgaaaaac
     1861 aaattaaatt caacgaagaa cacggcatcg tgccgcaaca gattaaaaaa caggtcaaag
     1921 acatcattga cggcgtgtac cacgaagaag acggtggtaa aggccgtaga caaggcaaaa
     1981 acaaggttaa agtcagcgaa attcacaacg aagaagacgc ggttaaagaa atcgccaaac
     2041 tggaaaaagc catgcagcag gcagctaggg atttgcagtt tgaagaggcg gctgtactga
     2101 gggataggat acgggggatt aaggaagggt tgttgtttgg gagtgagtaa taaaagcact
     2161 tgaaagttta gttgatgacc acaatgttct taaaagatct ggagaaacaa aagatgtgga
     2221 gaaacatcgt aacttgatca attacattct gaggactgaa aatgacatat tcttttcgtg
     2281 tagaaaatat aaatgatata tctgatattc ctgaaaatca tttggaaata gttagagctg
     2341 ctctgcctag taatagaagt aatgccttat ctcgtgagcc agatggggca caagctgttt
     2401 ttgtttatga aattgatggt gaatttagag ctaagttatt tgaccgcata aaagatacag
     2461 acagttattt acaagcaaca gaggataatt actctaatga ccctcgcagt ataggagtag
     2521 tatacgtttt ggagcaacag caagggtagc ctgagggccg cacaaactct caagctgacg
     2581 tgcagtatgt gtgatacgca cgcatgcgtt ttttcgttca aaccattgtt ttaaaccttg
     2641 tatcgaaaca tcactcgctg ctttatcttg aaaaaatgcc gtctgtaagc aaacatgctt
     2701 caaacagcat ttttttatcc ggcggtttgt tagcccatcc gtttgaatac caagtcccaa
     2761 acgccgtgcc cgaggcgttt gccgcgcgct tcgaatttgg tttcggggcg gtagtccggg
     2821 gtgggggcgt agtctgccgc cgtattttgc aggctgtcga agctactgag gacttcaagc
     2881 atctgccgtg catattcttc ccagtctgtc gccaggtgga tatagccgcc ggttttgagt
     2941 ttgggcagca gtttggcgat gaagggggct tgtatcagac ggcgtttgtg gtggcgtttt
     3001 ttgtgccacg ggtcggggaa gaatatgtgg atgccgtcga gcgagccgtc ttgcagcata
     3061 ttttcgataa cttctacggc atcgtgccgc atcacgcgga tgttttccaa atggttttcg
     3121 ttgatgagtt tgagcagatt gcctacgccg ggaccgtgta cgtcgatggc gaggaagtcg
     3181 gtttcgggca ggcggcgggc gatttctgcg gttgccgtcc ccataccgaa gcctatttcg
     3241 aggattttgg gtcggttgct tccgaaattg gcatcaagat cggccggtgt tgcttggtaa
     3301 tcgatgccga agctgtccca taaggtatcg atggcgcgtt gctgtgcggc ggtcatatgg
     3361 ctttggcgga ggacgaagct gcggatgctg cggggatgtt cggcttgcca gttgttttgt
     3421 gtttggtttt cggcggtgtc tgtcataatg cttccgtgcg cttgtgcaat caggatgccg
     3481 tctgaatgtg aaaggcctgt tcggacggcg ggaagggaaa ggggcgatta tacctgtttc
     3541 gtttcggcag ggtaggtgta acgctgttca gacggcattt tgccattgtt gcagcagttc
     3601 ttgcaggacg cttttttgcc cgtatccgtt gtttatggct tcttgcaggt agcggcaggc
     3661 tttttctttg tccggcccga caccgtgtcc gctgtaatgg atgcaggcga ggttgtagaa
     3721 cgccatactg tctttttgcg cggcggcttg ttcaaaccat ttgcgggctt tgccgtagtc
     3781 ggcggctatg ccctgtccga agtagtacat actgcccaga ttggtttggg ctttgctgtg
     3841 tccgagttct gcggcttctg tgtagagttt gagcgcggct gcgtattcgg gcgcacattg
     3901 taatccgtaa tgcagggctt gggctttttg ataaagctgt tctgcccttt gccgcctgat
     3961 ggcggaatct ttgatgccgc cgtattggtc gggatggttt aacgcgctgt cggatatgag
     4021 tttctgatag gcggaaaggc tgccggcttc ggctgcctgc cggtaataat gccgggcttt
     4081 ttccgtatcg ggggctatgc ccaaaccgta gcggcagatg tcgcccagta tccgcaaacc
     4141 ttcgggatgg cgttctgctg cggctgcttc ggcatgatgc agggcaagtt tgtggtttct
     4201 ttcctgatat ttgccttgta ggtagatgtc ggccagtgcg gcatgggcat cggggtcatt
     4261 ttcctgtgcc gcctgttgga accggtaaac ggcttcatcg ggagccgtag gtaggatgag
     4321 ttcggcaagc ggggtgtagg cgggaatgta tccttgttgc gcggcggcgc gcaaatgatt
     4381 gacggcctgt gccgtgtcga ctttcgtccc ttgcccgaaa cggtattgca gcccgagctg
     4441 ccaatgtgct tccggcaagc cttgtgccgc cgcttttcgg tagagcaggt gcgccgtttc
     4501 aaaatccggc ggtgtgccgt accggcagta ttgggcgagc agatattgtc cgtgggggtt
     4561 gttgtttttt gcgctggttt ttgcccattc gagtgcttct tcaaaatgcc gggacgcgta
     4621 cagcaggtac atcaggcgga cttgcgcttc gggcactcct gcttctgccg gaggcctgta
     4681 aagcgatatc agggtttcag gggggcgcgc cttccaattc ttggatatat gccagattgt
     4741 aacgcgcgta gggatgcccc tgttcggcgg cgatgcggag catttccata ccttgttcgc
     4801 ggtcttggta ggggctgtcc gcatccatca ggtgttttgc caactcgaat gcggcggcgg
     4861 gatgacctgc caaggccgct tctgtcagca gtcggcggac ttcggtaaag ttatattgtt
     4921 tttgagaaag gcattctaag gtctgccgat agggtaagtc gggattgttc atatcagatc
     4981 gttttccgtt ttgtcgaatc atcgttatat gttatgaagt taggtttata tcggcacgcg
     5041 ggcctgctgt atcatatttt tacagacggt ttttgttttg gcggcatact aatgcagatg
     5101 attataaatg gtttccgcca atgccttgct gatgccttcc actttttcca agtcctcgcg
     5161 gctggcggca atcacgccgc gcaggccgcc gaagcgggtg agtagggctt ggcggcgttt
     5221 gctgcccacg ccggggatgt cgctgaggga cgaggtaacg cgtgctttgt cgcgtttttt
     5281 gcggtggcct gtaatggcaa agcggtgtga ttcgtcgcgc acggtttgca gaaggtgcag
     5341 ggcggggctg tgcgggggca ggcggaagac ttcgccggta aaaggcagta tgagctcctc
     5401 cataccggct ttgcgctccg ggcctttggc gataccgacc aaaggaatgt gcagcccgag
     5461 ttcttcccat accgatacgg ctacgccgat ttgccctttg ccgccgtcaa ttaacacggc
     5521 atccggccat ttgacggtct cgccgttggc ttcggcctcc tgcattttgc cgtaacggcg
     5581 cgtcaacact tcgcgcatgg cggcgtagtc gtcgccgggt ttggcggtcg tgatgttgta
     5641 gcggcggtat tgcgaaggct ggatgttttg ctcatcgtac acaacgcagg acgcaatagt
     5701 ggcttcgcct tgtgtgtggc tgatgtcgaa acattcgagg cggttgaggc cgtctgaatc
     5761 cataccgagg attttggcga gttcgtcgat gcggtgttgc tggctgcttt gttgcaggcg
     5821 gcgttgtgca atcgccattt gtgcgttttg ttccgccatt ttcaaccaga ctttgcgttc
     5881 gcctatggtt ttggtgacaa attgcatctg cttgccgtgt tcgccttcca aagcctcttt
     5941 cagcgcatcg ggaacggaaa agttgctaat aatgatgtcg ggtttgcttt tgcccaaata
     6001 atgttgggcg acgaaggctt cggcgtaatc ttgtccgttt ggatcgggat cgtttttggt
     6061 gtcggggaaa aagcttttgt cgccgacgtg ccgcccaccg cgtatgctga cccagtgtac
     6121 gcagacgatg ccgtctgaaa ccgccagcgc gagcaaatcg atatcgttgg ggttgttcgg
     6181 gtttttgctg tcgatgaact ggttgctctg cataatgccg agtgcttgga tttggtcgcg
     6241 gtagcgggcg gcttcttcaa attgcagatt cgcggcggca gtttgcattt tgtgttgcag
     6301 ggtgcgggtc agttcgtcgg ttttgccgtt gagaaaagtg gcggcttgac gcacgctgtc
     6361 gcggtagtct tcttcgctga tgtgtccgac gcagggcgcg gtacagcgtt tgatttggta
     6421 cagcaggcaa gggcggtcgc ggtgttcgaa cacgctgtct tcgcaggtac gcagcataaa
     6481 gactttttgc aggacttgga tgctgtcgcg cacggcgttg ctgttgggat acgggccgaa
     6541 gtattggttg ggctttttca gcgtgccgcg gtaatacgcc atttgcggat attgatggcc
     6601 gctgagcatc agataaggat agcttttgtc gtcgcggaaa agaatattgt atttgggcga
     6661 cagggctttg atgaagttgt tttcgagaat cagggcttcg gattcggagc gggtgatggt
     6721 ggtttcgatg tggcgaacct gttttaccat caatgcgatg cgcggcgaat ggtcgttttt
     6781 ttggaaatag ccggacacgc gccgcttgag gttgaccgct ttgccgacgt ataagacttt
     6841 gccgccttcg tcaaaaaaac ggtacacgcc cggcagcttg ggcaggtttt tgaggaagag
     6901 cgggatgtcg aattgttccg gagaacggtt ttctgtattc atatcaatca tttgaaaaac
     6961 ggcttcagac ggcatatcgg cagcggcagc cttatccgtt cggaaaagct gtcgggtggt
     7021 tatatgcggt caatcgattt ttacccgttt caggcgcaag gcattgctca acacggaaac
     7081 cgagcttgcc gccatggccg cgcctgcgat gacgggattt aaaaagccaa gcgcggcgag
     7141 cggaatgccc aagatattgt agaagaaggc gaaaaacagg ttttgcttga tgtttttcaa
     7201 agtggcttgc gacaccagca gggcatcggc gagctgatta accgaatgct gcatcagcgt
     7261 ggcggacgcg gtgtgttcgg caacgtccgc accgcctttc atggcgaagc tgacgtttgc
     7321 ggcggcgagc gcgggcgcgt cgttgatgcc gtcgccgacc atcgccacgg ttttgccggc
     7381 ggctttgagt ttctgcactt cggcggcttt gtcgcgcgga ctcatattgc cgaaggcgtg
     7441 agcgataccc agttgttggg cgacgtattc gaccgtgcct tggttgtcgc cgctcatgat
     7501 gtaaacatcg atattgtgtt ttttcagacg gcctatggct tcggcggtat cggctttcaa
     7561 cgcgtcggca agtgcgaatg caccgatagg tttgttatcg actgagactg caacaatgct
     7621 tgcaatgtcc caaacgccgt ctgagaactc gggcaaggtc agttcggcaa attcggcttt
     7681 gcccgctttc accaatcccg caccttccac ttcggcggca atgcctgcgc cgacaacggt
     7741 ttgcgcgttt tgtgcggcgg gaatctccaa accgcgcgct tgggtggcgg aaacgatggc
     7801 tcgggcaagc ggatgggcgg cgttttgttc gatggcagcg gcgatgcggt acaaatcgtc
     7861 tttgtcaaag ccgctgtcgg gaacacaata aacggcggca acctgcggcc tgccttcggt
     7921 cagcgtgcca gttttgtcca acacaacggc atcgacgtgg gcggcttctt ccattgccgc
     7981 tgcgtcttta aaccaaatgc cgtgtttcac tgctttaccc atgccgacca taatcgcggc
     8041 gggtgtagcc agaccgagcg cacacgggca ggcaatcacc aaaacggcga cggcgtgcat
     8101 cagcgcaacc gtccaatcgc ccttaatcag ccaagtaaca ataaaagtca acaacgcgat
     8161 gcccacgacg gcaggcacga acaccgccgc cgccttgtcc gccacgcgcg caatcggtgc
     8221 tttactgcct tgcgcttcag agagtgcgtt catcatgtcg ccgagcaggg tttggctgcc
     8281 gagctgcgcg gcgcggtaca ccacgctgcc ttcggtcatc agcgcgcccg ccaacacttt
     8341 gccgcccgcc tttttctctt cgggattgga ttcgccggta agatggctct cgtccgccca
     8401 gccgctgccg ctttcgataa cgccgtcggc ggcaatgcgt tcgccgtggt tggcgcggat
     8461 aaggtcgccg atttgcactt ggtcgatggg aagctgtttc cattcgccat cgcgctgcac
     8521 gttgacttgg gtcggcgtga gtttgagcag caagcccaag ctgttcaggc tggatttttt
     8581 ggtacggtgt tccaaaaatt tgcccagcga cacaaaacca atcaccatca cgcccgcttc
     8641 aaaatacacg tgcgccattc cgtgtgccgc gtgcggactg aaaaacaaca tataaacgga
     8701 atacaggtaa atcgacaccg tgccgatagt aaccagcacg tccatattcg ccagcccgcc
     8761 tttaatgctc gcccacgcgc ttttgtaaaa cgggattgcc agccaaagtt gcacaatgct
     8821 tgccagcgca aactgccaca acggcggaat catccaatcg tgcctaccga tcatcatccc
     8881 cgccataccg acaaggaacg gaatattgat ggcggacagc agccacagcc gccagccgat
     8941 atggtgttct gcttcagctt gcggcaatgt atcttccgtt ttttccttcg cgccgtaacc
     9001 ggttttctca atgattttgg caatgtcggc tactgaggtt ttgctgtcgt caaacactac
     9061 ctgcgcctct tcgctggcga agtttacccc cgccgattcg acaaaatctt ttttgttcaa
     9121 cactttttca atgcgcgaag cgcaggcctg gcaggtcatg ccttcgattt ggaaacggat
     9181 tttttgttgc ataagcgtga ttcctgcttt gatattcaga cggcagttaa ggctgcggga
     9241 tgccgtctga acgccggtat ggtcgaaatt tctcaaacgc gccgataatt cgagtagaat
     9301 ccgaaaactt gacacagcaa aggaaaaaca atgtcccaac accgcaaact gattattttg
     9361 ggttccggcc ccgccggata caccgccgcc gtctatgccg cgcgcgccaa tttaaacccc
     9421 gtcattatta caggtatcgc gcaaggcggg caactgatga ccaccaccga agtggacaac
     9481 tggcctgccg atgccgacgg cgtgcaaggg ccggaattga tggcgcggtt tctcgcccac
     9541 gccgaacgtt tcggaacgga aatcattttt gaccaaatca acgccgtcga tctgcaaaaa
     9601 cgcccgttta cactcaaagg cgatatgggc gagtacactt gcgatgccct gattgtcgca
     9661 acaggcgcgt ccgccaaata cctcggtttg ccgagtgagg aagcgtttgc aggaaaaggc
     9721 gtttccgctt gtgccacctg cgacggtttc ttctacaaaa accaagatgt tgccgtagtt
     9781 ggcggcggca atacggcagt tgaggaagcg ctctaccttg ccaatatcgc caaaaccgtt
     9841 accctgatcc accgccgcag cgagttccgt gccgaaaaaa tcatgatcga caaactgatg
     9901 aaacgcgtgg aagagggcaa aatcatcctc aagctggaaa gcaacctgca agaagtactg
     9961 ggcgacgatc ggggcgtaaa cggcgcatta ttaaaaaaca acgacggttc tgagcaacaa
    10021 attgccgtca gcggcatttt tatcgccatc gggcacaagc cgaataccga tattttcaaa
    10081 ggtcagttgg aaatggacga agccggttac ctgaaaacca aaggcggcac ggcggacaat
    10141 gtcggcgcaa ccaatatcga aggcgtatgg gcggcgggcg acgtaaaaga ccatacctac
    10201 cgtcaggcga ttaccagcgc ggcttccggc tgtcaggcgg ctttggacgc ggaacgctgg
    10261 ctgggcagcc aaaacatttg aatgccgtct gaaaccgaaa ttttcggcgg aatgcaaatt
    10321 tttcgcacca aatccttgat tccgtcggca aaccccaata taatgcctgc tctttgaacc
    10381 ttgccttctg ctttcgcatg gcagaaggct gttttaccat ccataaggag ataacatgcg
    10441 tcattacgag atcgtgttta tcgttcatcc tgatcaaagc gagcaagtgc ctgctatggt
    10501 tgaacgttac aaaaccatga ttgccgaagc aaacggtaag attcaccgtt tggaagattg
    10561 gggtcgccgc caactggctt acccgattaa caaaatccat aaagcccatt acgttttgat
    10621 gaacatcgaa accactcccg aagtggttga agagctggaa accgcattcc gcttcaatga
    10681 tgccgtattg cgccatctaa ccatcaaaac caaacacgcc gttactgaag catcccctat
    10741 gttgggtggt gaaaaggcta agaacctgtt gagcggtgcg tctgaagaag cggtcgccca
    10801 ataattggga ttcaataatc ttgtttcgct tgccgcgtta attgaaaagg ttttccctat
    10861 tcgatatacg cctgccggaa tccctgtttt agatattatt ttaaagcacg aatcgtggca
    10921 ggaggaaaac gggcagcaat gccttgtcca attggaaatt ccggcacgga ttttaggcag
    10981 gcaggcggaa gagtggcagt atcggcaagg tgtatatgtt cacgtcgaag gttttttagc
    11041 tcaaaaaagc agacgttccc ttatgccgat gctcaggata caaaatattc aagaatataa
    11101 aggttaaacg acaatggctc gtcaatcatt caaacgtaga aaattctgcc gtttcacggc
    11161 tgaaaaaatc caagaagtcg attacaaaca agttgatttg ctgaaagact ttatctctga
    11221 aaacggtaaa atcattcctg cacgcatcac aggaacgaag gcattctacc aacgccaatt
    11281 ggctgttgcc gtaaaacgcg cacgcttcct ggctctgctg ccttacaccg accaacacaa
    11341 ataattttgg agattgaatc atgcaaatta ttctgttaga aaaaatcggc ggtctgggca
    11401 acttgggcga tatcgtaacc gttaaaaacg gctacgcccg caactttcta attcccgcag
    11461 gtaaggcaaa acgtgcgacc gaagcgaata tgaaagagtt tgaagtacgc cgcgccgaat
    11521 tggaagccaa acaggctgaa attttggcag atgcccgagt ccgtcaggaa aaattggacg
    11581 gtcaaaccgt taccgttgct caaaaagccg gtgtggacgg tcgcctgttc ggttccgtta
    11641 ccaatgccga cattgctgct gcaatcgttg ctgccggcat cgaagccgtg aaagcaaatg
    11701 tacgtctgcc gaacggtcct ctgaaagccg ttggcgagta cgaagtggaa gtggctttgc
    11761 acactgatgc cgttgctaaa attaccgttg ccgtcgttgc cgcaaccgag taattgaaat
    11821 tatcagatgc cgtctgaaac cctgccgatt gtcggaatgt ggtttcagac ggtatttgtt
    11881 ttgttttccg tatattctgc aaaagtcggg atggttgttt ttatagtgga ttaaatttaa
    11941 accagtacag cgtacctggc ttcaatatac tcaataggat aggtctcttc ctaaccgaaa
    12001 aaataattgt cttttccctt gcaaataatt gaaatcaaca gattactaaa acacagccaa
    12061 aatattcaat tgacaaaaac ttttctgatt ttgagaaatc tgtgaaaatc aggggattat
    12121 ttattttgaa aatggtatcg ggcataaaat ttggctgtgt ttcaatatgg tgctgataac
    12181 atactttgtt tcaaaatgtt caataggata acttcctgat tgaccgaaaa ataatggcct
    12241 tttcccttgc aagcagttga aatcaacaga ttgtcaaaat acatcctaaa aatttaataa
    12301 cttatatata tgattgcaaa ggttgcggct tgtatgccgg tcattatggc ggaaataccg
    12361 tttgggaggc agccgcatcg cgtggaatcg gcacggcagg cgatgcgccg atgccttcgg
    12421 gcgtgaaggc ggggcaatcg gcagtttgga aaaaggcaat ggaatctgtg ggcagaagaa
    12481 gttaagggta aaagtcgggc gcacggtcag gttgtctgtc cttttgtcgg aacaggagac
    12541 tgggcagctt tgtcctgatt aattgcttgt gcaaacggta aaaaggtttt ttcgtgtgtt
    12601 ttaaagcagg ttgtacagca tttttccggc aatcaaaagc agcataatgc cgaaggattt
    12661 tttgagtttg gcagaagaaa gtttgtgggc ggttttgaca ccgagcgggg caaaggcaat
    12721 ggttgccgcg ctgaggacgg cgacggcggg caggtaaagg aagcccagtg acccttcggg
    12781 caatcctgca atattcaggc cgttgagcag atacgatatt gcgccggaga gtgcaatcgg
    12841 ccaggcaagg ccggatgatg tgccgatggc tttatgggcg gggaagccgc agtggattaa
    12901 gaaggggacg gaaagtgaac cgccgcctat gccgacccag ctcgacattg tgccgaacag
    12961 tgtggaaacc gcagtcagtc cgggcagctt gggcagcggg cgggatgccg tctgacgacc
    13021 ggtatgcagt gttttgaatg cgacggcggt taaaaacagg atgaagaaaa tttgaagccc
    13081 gaacgcgggg atatattttg cggagagtgc gcccgtgaat acgccgaata tcatacccgg
    13141 catcatcgca aatacggttt tccagtcgac cgcccgtttt ttgtgctgcc ccaacatact
    13201 ggaaaaggcg gtgaagacca tgacggcgaa ggatgtgccg acggcgaggt gttgcgcgta
    13261 aggatgttgt gccaaaccct gcaaatcaag cacccataaa acgacaggga caatcagcgt
    13321 gccgccgcct acgccgaaca gaccggcaat aaaacctgcc gcactgccta cggcaagcag
    13381 gattaagata atgtcccaat gccacattta atccgccttt ctgtattttt tccaaaatcg
    13441 gcgtgccgcc ataatgtagc cggacaggct gtatccgaga aagaacagga agaggacgag
    13501 cgacggttcc caagtgacca gaagcagcag taagacggca agcagcattc cgacaaaggg
    13561 gacttgtctg cggatgttga tttctttaaa actccaaaaa gggatttgga caatcatcga
    13621 caggccggca aacagtgtga tgcccaatgc ccaccagtgg acggcgggga atttttcgac
    13681 gctgtggttg acccaaatca gcccgacaat cagcgcggcg gcagtcggac tgggcacgcc
    13741 gataaaccag cgtttgtcca ccttgccgat gagtgtgttg aacagggcga ggcgcagggc
    13801 ggcgcaggcg cagtagatga aggcgacgga ataaccgatt ttgccgaact gccaaagctg
    13861 ccatttgtag gcaatcagag cgggagcaac gccgaagctg accatatcgg caaggctgtc
    13921 gagctgctcc ccgaacgcgc tttggctgtt ggtcagccgc gccacgcgcc cgtccatacc
    13981 gtccagcaac atagagatga ataccgcgat ggcggcggtt tcataacgtc cgtgcatgga
    14041 ttgggtgatt gcataaaatg cggaaaacag cgcggcgatg gtaaaggagt tgggcagcag
    14101 gtagatactg ttttgacgca gggagcgttg cggggtgttg gtcggggttt ccatagctga
    14161 agtgtctcac ggacgtggag aatcgatagg ggattataac ccaaaccatt atcgaacaat
    14221 acgcccttta ggatgaagcc ctcggaggcg gtttgttttt ctgatggcgg atgctttgcg
    14281 gggcggtttg actctgcctg ccaccgggtg tgttgtttcg gatttttgcc ggatggatat
    14341 tgttgccggg catttctggg cttgccggaa ggtatcgggg ggagggcatc ggaatatgcg
    14401 gcgcaccgtt caaaccgggg cggcgtttcc ctattttcag ggggttgcag gtttcaacaa
    14461 attgaaagat attttttgca tttccgaccg tatgctgatt ttgaagcggg ataaattata
    14521 ggacaaggtt tttcgcccgc cgcaaaggtt ggccgcaatg ccgtctgaaa acaacggctt
    14581 gccgctaagg cggcggtatg cggatttgcg ctgccgaaat cggtttgcca tacccggcgg
    14641 tgcaatctgc ggggagggat aagccgataa ggcgggactt atcctgtttt ttgtttataa
    14701 tgcaacagaa tttggacgaa ccgtcgggaa tttatcatga tgaaaaagat actggccgta
    14761 tcggcactat gcctgatgac tgcggcggca cgggctgccg atacttacgg ctatctcgcc
    14821 gtttggcaga atccgcagaa tgcggacgat gttttgcagg ttaaaaccac aaaagaagat
    14881 tcgacgaaaa gcgaagcgtt tgccgagttg gaagccttct gcaaaggtca ggacacgctt
    14941 gcgggcattg ccgaagacga gccgaccgga tgccggtcgg tcgtgtcgct gaacaatacc
    15001 tgtgtcgcgc tggcataccc gaaagccttg ggcgcgatgc gcgttgaaaa cgccgtcgtg
    15061 attacttctc cgcgttttac gagcgttcat caggtcgcac tcaaccagtg catcaaaaaa
    15121 tacggcgtac agggacaatg cggcttggaa acagtgtatt gcacatcttc ttcttattac
    15181 ggcggaactg tgcgctcttt gattcaaaat ctcaaataaa acggaaaatg ccgtctgaaa
    15241 gatgttcaga cggcatttct atatcgacgg tcaggattct ttcggatcgg gcagcaggct
    15301 gttcaacata atggcaagta cggcgcacaa gcccacgccg gcaaagctga agctgcccaa
    15361 tttgagcgtc atgccgccga tgcccgtggt cagtaccgag ctgacgatga ccaggttttt
    15421 cggcagcatc aaatcgactt tggcatcaat caacgttttc acgcccaaag aagcaatcgt
    15481 gccgaacagc agcagcataa tgccgcccat tactggcatc ggaatggaag ccaaaaacgc
    15541 attgaatttg ccgaaaaacg ccatgcagac ggcaaaaacc gccgcccaag tcatgatgac
    15601 ggggttgctg tttttggtaa tcatcaccgc acccgttact tcgccgtagg tcgtaaccgg
    15661 cgggccgccg atcagacccg caacgcatac gcccaaaccg tcgcctgcaa gggttttgtc
    15721 caagcccggg tctttcgtat agtctttccc cgtcacattg ccgattgcca tgatgccgcc
    15781 gatgtgttcg atggcggggg cgacggcaac gggcagcata aacagtgcag cctgccagtt
    15841 gacctgaggc gtttcaaaat ggggaacggc gaaccagggc gcgtgtgcaa tgcttgccgt
    15901 gtccaccagt cccatcagca gtgccaaaac ataacccgaa gcgacaccga tcaagatggg
    15961 aatcagcttc atcatcctgc tgccgaaaac cgatacgatg gcggtaacgg caaaggtaaa
    16021 gccggaaaga atcagcgaat cggtatagtc gatgacctgt ttgccgtccg cctgacccat
    16081 tgccatgctg cttgccgcca cggcaacaga cagaccgatg accatgatga cggggccgat
    16141 gacgaccgga ggcagcagtt tgtgtaccgc tgccagtccg cgccaacgga tcagcgcggc
    16201 aaacacaaaa tacataaagc cggcggcaaa cagtccgaac atggtggaag gcagccccca
    16261 ttcgccgacg gagtagataa tcggtgcgat aaaggcaaac gaagaaccaa gaaaaatcgg
    16321 cactttgcgt ttggttgtga tttggaacag cagcgttccc aagcctgcgc ccaaaagcgc
    16381 aagagccgga ttcagaccgg tcagcagggg aaccagcacc attgcgccga atgccacaaa
    16441 taaaatctgc gcgccggaaa cggcaagttt cagttggttc ataccatctt ctttcaatcg
    16501 gttcaaacgg cacggattat actggtttgc cgagggtctg cgtaaaaata catatggcgg
    16561 gcaacagata tgccgtctga aaggcaatgt ctgtctgttg caggatgaaa gcaggagaaa
    16621 tggagtataa tccgacactt tccatattca atccgcaagc cgtccggctt ccttatttca
    16681 gacggcattt tccggtaata cggcatgaca gaaaaatccc ataaaaaaac agcaaaaggc
    16741 agggcgggaa gcccgtcccc aacgtccgca cgcaacaaga aagccgacaa cggcgcacgg
    16801 ggcaataaag tttccgagcg gctcaaggcg gtcaaagagt tgcagaaaac cgaaaccaaa
    16861 aaggcgcgtc ccgaacatgt cgtcaacctt atcggcgacg cactgtggct gatgggtttg
    16921 gcggcaacct tgtatttggc gatttccctg attagtttcg atatgggcga tccgtcttgg
    16981 tcgcacagtt cgccggttgt ggaagatgtc gccaattggg gcggactgtt cggcgcgtat
    17041 gttgccgatg tcggctatta tcttttcggc tggtcgttct ggtggtggat agcggctgcc
    17101 tgcgtcatgc tgtataaaaa ttttcgtctg cacgcaaaac agacggaaaa cgaggcatac
    17161 aaccacaaaa tcgctgccgc cgcgctgttt gtcctgacgg tcttcagccc cgtcttggag
    17221 tattttgtgc tgggcggaaa atatgccgac tccctgcctg tcggagcagg cggtatggtc
    17281 ggcatacgcg tcggcgcagt gtttgcgtgg ctgctgggga aatcgggcag cctgctgatt
    17341 atcttggttg ttctgctgtt gtcgttgtcc ctgctggtgc agatttcatg gctggaattt
    17401 ttgaacggtg cgggcagggc ggttcaaaac cgcctgagtg ccttatccgg caaggtcatg
    17461 gctttgggaa aacgccggcc gaataccaaa acagacggtg tcgataccca aaatacacgg
    17521 cgcatggtaa aagaagccaa gaatattacg gccaaacccg ttgccttgcc cgaaggcagc
    17581 agcagcaacc gcaaatccgt cgcggtttcc gtcgcgccgt cgcccaaaat tcaggtttct
    17641 ctgtttgaag atgacgaacc tcggcaggcg ggcgaatacc acaagcctac attgaaccta
    17701 ttgcggattc ctgacagcga acccgtcagc atcaatcccg ccgaattgga gcgcactgcc
    17761 gaactgatcg aatccaaact ggcagaattc ggcatcggcg tacaagtcgt atccgccaca
    17821 tccggtcccg tcatcacgcg ctacgaaatc gaacccgcgc aaggtgttaa aggcagccaa
    17881 attgttgcct tgtcgaaaga tttggcacgc tctatgtcgc tgcaatccgt gcgtatcgtc
    17941 gaaaccatcg caggtaaaaa cacgatgggc atcgagttgc ccaacgacaa acgccaagac
    18001 gtgatgttga gcgaaatctt gtcctcgccc gtgtttgccg aagccaaatc caagctgacc
    18061 gtcgcgctgg gcaaagacat tgccggtacc cccgttgtcg gcgacttggc gaaaatgccg
    18121 caccttttgg tcgccggtat gactggttcg ggcaagtccg tcggcgtgaa cggcatgatt
    18181 atgtctatgc ttttcaaagc tacgcccgac gaagtccgct ttatcatgat agacccgaaa
    18241 atgcttgagt tgagcattta cgacggcatt ccgcacctgc tctgtcccgt cgtgaccgat
    18301 atgcgcgaag cggggcaggc gttgaactgg tgcgtcgccg aaatggaaaa acgctaccgc
    18361 ctgctttccc acgccggtgt acgcaatctg gaaggcttca accaaaaagt cgaagccgca
    18421 aaagcggcag gcaagccgct gctcaatccg ttcagtctga accccgacag ccccgagcct
    18481 ttggaaaaac tgccgatgat tgtggtcgtt atcgacgaac ttgccgacct gatgatgacc
    18541 gaacgcaaag ccgtcgagca gcaaatcgcc cgtctcgccc aaaaagcgcg cgccgccggt
    18601 atccatatga ttgtcgccac ccaacgtccc agtgtcgatg tcgttactgg cctgattaaa
    18661 gccaacatcc cgacgcgtat ggcgtttacc gtgcaaagca aaatcgacag ccgtaccatc
    18721 ctcgaccaaa tgggcgcgga cgaactgctc aaatatggcg attcgctgtt cctccagccc
    18781 ggcagtgccg aaccgacccg cctgcaaggc gcgtttgttt cagacgacga agtacatcaa
    18841 gtcgtcaact acgtcaaatc gcaagcccca gccgactata ttgaaggtct gctcagcggc
    18901 gaggccgcgc tggaaactgc caatatcgtt aatccgaatg cagacagcga cgaattgttc
    18961 gatcaggcag tcgcctatgt tttggaaagc aaaaaaacct ccatttcgtc tttgcagcgg
    19021 cagttgcgta tcggctataa ccgcgcggca aacctgatgg aggcactgga aaatgcgggt
    19081 gtcgtttctc ccaccgacct caacggcagc cgtaaaattt tggcgcacaa ggaccatttg
    19141 tagctcgtat tgcaaaatgc cgtctgaacg gcggaattgg cgtttcagac ggcatattat
    19201 gtttcaggcg gaacattgtg atatacttgc cagctaaaat ctcccctttg cggcaatgcg
    19261 gttcaaatat ccgtaccgtt gcgctgtttg cttccccatg cagggaagaa agtttaccat
    19321 tttatcaaca caacaaattt aagggcttat gatgagcgta actgttgaaa ctttagaaaa
    19381 tctggaacgc aaagtagtgt tgtccctgcc ttggtccgaa atcaacgcag aaaccgataa
    19441 aaaactgaaa caaacccagc gccgtgcaaa aatcgacggt ttccgcccgg gtaaagcacc
    19501 tttaaaaatg attgcccaaa tgtacggtgc gagcgcgcaa aacgacgtga tcaacgagct
    19561 ggtgcaacgc cgcttccacg atgttgccgt tgcccaagag ttgaaagtgg caggcttccc
    19621 ccgttttgaa ggcgttgaag aacaagacga taaagaatct ttcaaagttg ccgccatttt
    19681 tgaagtgttc cccgaagtcg ttatcggcga tttgtctgca caagaagttg aaaaagtaac
    19741 cgcttccgtc ggtgatgccg aagtggacca aaccgtagaa atcctgcgca aacaacgcac
    19801 ccgcttcaac catgtcgaac gcgaagcccg aaacagcgac cgcgtcatca ttgactttga
    19861 aggcaaaatc gacggcgaac cttttgcagg cggcgcatcc aaaaactacg ccttcgtatt
    19921 gggcgcaggt caaatgctgc ctgaatttga agccggcgta gtcggcatga aggctggcga
    19981 aagtaaagac gttaccgtca acttccctga agaataccac ggcaaagacg ttgccggtaa
    20041 aactgccgtg ttcaccatta cgctgaacaa cgtttccgaa gcgactctgc ctgaagtcga
    20101 tgcagatttt gcaaaagcct tgggtattgc ggatggcgac gttgccaaaa tgcgcgaaga
    20161 agtgcagaaa aacgtaagcc gcgaagtgga acgccgcgta aacgaacaaa ccaaagaatc
    20221 cgtaatgaac gcgctgctca aagccgtaga gctgaaagca cctgttgctt tggtcaatga
    20281 agaagccgca cgcttggcaa acgaaatgaa acaaaatttt gttaaccaag gtatggctga
    20341 tgctgccaac ttggatctgc ctttggatat attcaaagaa caagccgaac gccgcgtatc
    20401 tttgggtctg attttggcca aactggttga tgaaaacaaa ctggaaccga ctgaggagca
    20461 agtcaaagcc gttgtcgcca acttcgcaga aagctacgaa gatcctcaag aagtaatcga
    20521 ctggtactac gcagagcctt cccgcctgca agccccgact tctttggcga tagaaagcaa
    20581 cgtcgttgat ttcgttttgg gtaaagccaa agtaaacgaa aaagctttgt cttttgacga
    20641 agtgatgggc gcgcaagcct gattatcctg aaaatgccgt cttagggcag tttttgaaag
    20701 caccgaagcc gcttttaaag cccgctttgg tgcttttaca tcatgaaagg agacgaaatg
    20761 tcttttgata actatcttgt ccctaccgtt atcgagcaga gcggtcgcgg tgagcgtgca
    20821 ttcgatatct attctcggct tttgaaagag cgcatcgtat tcttggtcgg cccggtaacc
    20881 gacgagtccg ccaatctggt ggttgcccaa ctgttgtttt tggaaagtga gaatccggat
    20941 aaggatattt tcttctatat taactcgcca ggcggttcgg taacggccgg tatgtcgatt
    21001 tacgacacca tgaattttat caagcccgat gtatcgactt tgtgcttggg gcaggcagca
    21061 agtatgggcg cgttcttatt gtcggcaggc gaaaaaggca aacgtttcgc cctgcccaac
    21121 agccggatta tgattcacca gcctttaatc agcggcggct tgggcggtca ggcatccgat
    21181 attgaaattc acgcacgcga actgttgaaa atcaaagaaa aactcaaccg cctgatggcg
    21241 aaacattgcg gccgcgattt ggcagatttg gagcgcgaca ccgaccgtga taatttcatg
    21301 tccgccgaag aggctaaaga atatggtctg attgatcaaa tcttggagaa ccgtgcttct
    21361 ttacagtttt aataagaaat ctgagagaaa caaatgtcgt ctgaaaacag ttcaggacgg
    21421 catttgtctt atagggactg tatagggatg attttgttct ggttgcgttt ggagccgttt
    21481 tcaccatagt tggttttgac atattcctct acaatagcta ggaattcagt tgcaatatta
    21541 tcacctttca gtgttatttt gcgctccccg tccacataaa ccggcgcgac gggtgtttct
    21601 cccgtaccgg gaaggctgat gccgatgtcg gccaatttgc tttctccggg cccattgaca
    21661 acgcagccca ttacggcaac gttcagggat tcaaccccag gataaagggt acgccatata
    21721 gacatttttt ggcgcaggta attttgaaca tcttgtgcca gctcttgaaa tacggtactg
    21781 gtggtacgcc cgcagccggg gcaggcggta accatcggcg taaacgaacg caatcccata
    21841 gtttgtaaaa tctcttgccc gacgacgacc tcctgagtac gcgggctgcc aggttccgga
    21901 gtcagtgaaa tgcggatggt gtcgccgatt ccttcttgaa gcaagacgga taatgccgcc
    21961 gttgatgcga caatgccttt gctgcccata ccggcttcgg tcaaacccaa atgcagcgga
    22021 taggcgcaac ggctgcccag ttcgcggtaa acctgaatca aatcctgaac cgcgctgact
    22081 ttgcacgaca ggatgatttt gtcttcgggc agtcccaata gaacggcttt ttcggcagat
    22141 tccaaagcgg agacaatcag tgcttccttc gtcacttctt cgggcggttt cggcgcggaa
    22201 gaagcgaggt tggcatccat catacgtttg gcgaggcttt gatccaaaga accccagttt
    22261 acgccgatgc ggacggcttt atcgttttca gcagcagtcc gaatcataaa ggcaaatttt
    22321 tcatcgcctt ttacgccttt gccgacattg ccgggattga tgcggtattt ggacaatgct
    22381 ttgccgcatt cgggaaattc cgccaacagg cgttcgccgt tgaagtggaa atcgccgata
    22441 agtggtgtgg catagcccat atcgtccaag cggcggcgga tttcggcaac tttggacgcg
    22501 gcttcggggc tgttgacggt aatacgcacc atttcggatc cggcatcgct caattcctta
    22561 atctgcaatg cggtggcttt tgcatcggca gtgtcggtgt tggtcataga ttggataacg
    22621 acgggtgctt ctgaaccgac ggtaatatga tcgatgcgga cttgatgcgt cttgcggcgt
    22681 tggagtgtgt tcatatggtt tgaatctgtt tattgaccga tgaggacggt ttgcaattct
    22741 tccgagtagg ggaaattcgc ctgcaattgt gcttcatatt cgtatgccgc ctgtgcgttg
    22801 ccgagggctt tggcaatttt ccagcctagc agcaaatcat cggcctgaag gacttctacc
    22861 ctgctttggt attttttaaa gtagtaatcg gcatcgccca actgcccggc cagcattttg
    22921 gtgcgcgcca gttctttaaa tgcgggtggg aactgcggct gggcggcgag ggaacgtttc
    22981 aaataggctt ccgccaatcc gaattgcccc tgttttgcgc tgcatatgcc tttattcagg
    23041 ttggcaatat aaggggtcgg gtaggtgggg tcggccaggg ctttgtcgaa atatgccata
    23101 gattcggcag ggcggttgag cctgccgcac aggaaccagc cgtagttgtt gttgatttcg
    23161 gcactgtcgg gtttgatgga gagggcttgc cggaaacttt cctgcgcctt gtcgttaact
    23221 ttcaggtatt gatagatttc ggcacggacc agccaggcaa gctcgttttt agggtccgat
    23281 ttcagggcgt cttcaatact tgccgtcgcc tgacggtagt cctgaccgcg catatattcc
    23341 attgccaact gggttttgat attggaaacc tgattggctt tttctgcccg cgaggggcgg
    23401 taggaagtgc tgcacgcgcc caaggcaagg acaagtaata aagagattcg tttggatggc
    23461 ttaaaaggca taattacccc tgttgtccga ttaaaatctg ctgccatttt tgttggcggc
    23521 gcgttttatc ctgaacctgc cccgccaact gtccgcaggc ggcatcgatg tcgtcgccgc
    23581 gcgtttttcg tacggtaacg acaaatcctg cctgctgcaa aatatcgcgg aacacacgga
    23641 tgttctcatt gctggagcgt tcgtatccag agtttggaaa gggattgaac ggaatcagat
    23701 tgaacttgca gggaacatct ttgaccagtt cgatcagttc gcgcgcatgt tgcgccttat
    23761 cgtttattcc gtccaacatg acgtattcga aagtgatgaa atccctgggt gctttgacta
    23821 gatagcgttg gcatgcagcc atcaattctt tcaagggata ttttttgttc aacggtacga
    23881 tttggttgcg gacttcgtca ttggaagcgt ggagggaaac cgccaaagcc accggcatga
    23941 catcgcgcaa cctgtccatt tggggaacca tacccgaagt ggaaacggtt acgcggcggc
    24001 ggctcaaacc gtagccgtgg tcgtccagca tgatgcttaa ggcggtaacg acattgtcga
    24061 agttcgccat cggctcgccc atacccatca tcactacatt ggaaattacg cgttcgtttt
    24121 tcggtgtaac acccatcgct ttgtttgccc accacaattg cccgatgatt tcggcagcag
    24181 tcaaattgcg gttgaagccc tgccggccgg tcgaacaaaa tgtacattcc aaagcgcagc
    24241 cgacttgtga ggaaatgcag agcgtgccgc gatccgattc ggggatgaag acggtttcca
    24301 ccccgttgcc cgtaccgaca tccaaaagcc attttcgagt gccgtctgaa gatttttgag
    24361 acatcatcag cttgggaatt tcgatgcctg cctgttcgtt cagtttatgg cgcaacgatt
    24421 ttgccaaatc ggtcatttcg ttaaaatttt gcgcgccgga ttggtgcatc caacgcataa
    24481 cctgtttggc acggaagggt ttttcgccca tatcggcaaa atgtcgggtc agcccttgaa
    24541 ggtcgtagtt gagcagattg gttttcatgt gtttgtgttt ttcttaaatt aaggcttcaa
    24601 ataaaaaatt gcagggcagg caagtttgat ttgaagcctt ttgcgacaga gttttcagac
    24661 ggcatggata tatttatgcc gtctgaagag gagttgtgcg gtgtattgta tcaacggggg
    24721 cagatttcgg tttggctgaa aaagtaggca atttccaaag cggcattttc cacgctgtcg
    24781 gaaccgtgta cggcattaat gctgaccgaa gtggcaaagt ccgcgcgtat cgtgccttcg
    24841 gcggcttcgg aaggattagt tgcacccatc agttcgcggt ttttcaggac ggcgttttca
    24901 ccctctaata cctgaatcat aaccggaccg ccggtcataa attcaaccaa tccggcgtag
    24961 aaggggcggt ctttatgaac cgcataaaat tcttgcgcct ctttgagagt aagctgcttc
    25021 attttggcgg caacgatttt cagaccgttc tcctcaaagc ggctgtatat tttgccgata
    25081 acatttttgc cgacggcatc gggtttgatg atggagatgg tacgttcaat cgccatgcta
    25141 tatccttatt tttacttaag aagaatcaaa tcggtattct atcaaaaaaa taacttatcc
    25201 gcctgaagcc ttgggagata gggtcaggtg cggctgttcc aaataaactt cggactgcat
    25261 ttcgaccatc cggctggcgg tgcgggtaaa ttcttcggca aaatcgcctt ccgtatagat
    25321 atggttgaca tctaccgcgc cggtggcaca cagtttgacc cggaaatcgt agagtacgtc
    25381 aatcagccaa gtcagccgcc gcgcctccgc cttttcttgc ggtgagagtt gttccaaacc
    25441 tgaaataaaa accatttcat aatgttcggc caaatacaga tagtcggact gtgagcgggg
    25501 gccgaagcac agtgcgcgga aatcaaacca tatggcacgg ccggactcgg ctttgtgggg
    25561 aatctcccga ccatggatgg tgctgatgcc ggggttcaaa tcggtaatgc ctgtcatttc
    25621 tttgaacagt tttgccagtt ttgcctcatt ttcttcattg gcaggcgtaa agaaaatctc
    25681 ggcggggcgg agggtacgca gtcggtagtc ttcaccgccg tcaacgttta agacggtcag
    25741 gctggactcg atgagcgcga ttgtgggaag aaaactgctc cggttttgac cttgcgggta
    25801 gagttcggaa ggcgcgtagt ttgaagtcgc caccaaaaca acgccctcgt taagcaggtt
    25861 ttccagcaga cggcctaaaa tcattgcatc cgcaatatcg ctgacatgaa attcgtcaaa
    25921 acataatacg cgggtttctt tggcaatctc ggcggcaacg gatttcaacg ggttgctttc
    25981 gcttttcagg gttttcagcc tctggtggat ttctgccata aaggcatgaa agtggacgcg
    26041 gcgtttgcgg cggtaaggga ggcagccgaa aaaagcgtcc atcagaaagc ttttgccgcg
    26101 tccgaccccg ccatagaaat aaagcccttt ggggacttgc ggggaacgca aactcctgcc
    26161 taaaaaacgg tttcttttgc gtttgaacat catcaattcg gtccaaagcc gatcgaggtg
    26221 ttcgatggcg gctgcctgcg cgtcgtcgcg gatgaagttg ggcagttgtg aggcagcctg
    26281 ataccaggtc agcgggctgt ggttttcaaa cggcggggct ttaaaaagtt ggtctctatt
    26341 actcatattt aaccttgtta tacttcttgc ggcggagaaa gaaaatgctt accgtcggta
    26401 ttataaatga tttccttcaa acttggcatt ttccgtggaa aagaataatg acgcgcacaa
    26461 gggggtataa gcaaaggcgg cgtttcaata tggtgcagat atgagctcaa atgcctttta
    26521 tagt
//
LOCUS       DAWFAD010000009        26280 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWFAD010000009 DAWFAD010000000
VERSION     DAWFAD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.009"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            182..538
                     /locus_tag="WGO49_000274"
     CDS             182..538
                     /locus_tag="WGO49_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ0305436.1"
                     /translation="MLSAYFPVFVFILVGLAASVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            529..1011
                     /locus_tag="WGO49_000275"
     CDS             529..1011
                     /locus_tag="WGO49_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ0305437.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            1024..1617
                     /locus_tag="WGO49_000276"
     CDS             1024..1617
                     /locus_tag="WGO49_000276"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ0305438.1"
                     /translation="MASIQNLYETVSRVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEAWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            1607..2863
                     /gene="nuoD"
                     /locus_tag="WGO49_000277"
     CDS             1607..2863
                     /gene="nuoD"
                     /locus_tag="WGO49_000277"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ0305439.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            2863..3336
                     /gene="nuoE"
                     /locus_tag="WGO49_000278"
     CDS             2863..3336
                     /gene="nuoE"
                     /locus_tag="WGO49_000278"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ0305440.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            3726..5027
                     /gene="nuoF"
                     /locus_tag="WGO49_000279"
     CDS             3726..5027
                     /gene="nuoF"
                     /locus_tag="WGO49_000279"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ0305441.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGKMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            5027..5464
                     /locus_tag="WGO49_000280"
     CDS             5027..5464
                     /locus_tag="WGO49_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305442.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICRKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINKL"
     gene            5754..8015
                     /locus_tag="WGO49_000281"
     CDS             5754..8015
                     /locus_tag="WGO49_000281"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ0305443.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIIQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAN
                     GLGVKNFATRLRQQDKRLSDDLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKKLSGNAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTADGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPLWKVLRVLGNLFDLQGFEYHDTAAI
                     LKDALDAESLPSKLDNRVSSTQKDFQTASSRLVRVGGVGIYHTDAIVRRSAPLQATVH
                     AAVPAARVNPNTLARLSLQDGQIAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            8018..9094
                     /gene="nuoH"
                     /locus_tag="WGO49_000282"
     CDS             8018..9094
                     /gene="nuoH"
                     /locus_tag="WGO49_000282"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ0305444.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVILGVWMISPLNLWK"
     gene            9175..9654
                     /gene="nuoI"
                     /locus_tag="WGO49_000283"
     CDS             9175..9654
                     /gene="nuoI"
                     /locus_tag="WGO49_000283"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ0305445.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            9666..10337
                     /locus_tag="WGO49_000284"
     CDS             9666..10337
                     /locus_tag="WGO49_000284"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ0305446.1"
                     /translation="MTFSAILFYILAAIVLYGALRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMEPADQVKVRADQGRMRLVKMEAVKPQTESAEESEVSDDLKPK
                     EEGKA"
     gene            10334..10639
                     /gene="nuoK"
                     /locus_tag="WGO49_000285"
     CDS             10334..10639
                     /gene="nuoK"
                     /locus_tag="WGO49_000285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ0305447.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLDELKG"
     gene            10673..11248
                     /locus_tag="WGO49_000286"
     CDS             10673..11248
                     /locus_tag="WGO49_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ0305448.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            11372..11713
                     /locus_tag="WGO49_000287"
     CDS             11372..11713
                     /locus_tag="WGO49_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305449.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVSPK"
     gene            11762..13786
                     /gene="nuoL"
                     /locus_tag="WGO49_000288"
     CDS             11762..13786
                     /gene="nuoL"
                     /locus_tag="WGO49_000288"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ0305450.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKIGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQTATIQ
                     LFPSVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMMIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPVTWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHGLGKNDNPHESPLVVTLPLILLAIPSVIIGYVAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQAFRPIYVLFESKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGVIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            13875..15371
                     /locus_tag="WGO49_000289"
     CDS             13875..15371
                     /locus_tag="WGO49_000289"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ0305451.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAILAVVVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            15381..16826
                     /gene="nuoN"
                     /locus_tag="WGO49_000290"
     CDS             15381..16826
                     /gene="nuoN"
                     /locus_tag="WGO49_000290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ0305452.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRAHPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            16938..17237
                     /locus_tag="WGO49_000291"
     CDS             16938..17237
                     /locus_tag="WGO49_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ0305453.1"
                     /translation="MTASMYILLLLALIFANAPFLTTRLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(17346..18242)
                     /locus_tag="WGO49_000292"
     CDS             complement(17346..18242)
                     /locus_tag="WGO49_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ0305454.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGEAVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIKYGEANALLTGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSRGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGATACPDLSDAELSVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(18226..18450)
                     /locus_tag="WGO49_000293"
     CDS             complement(18226..18450)
                     /locus_tag="WGO49_000293"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ0305455.1"
                     /translation="MKKITPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGPKELNLESDE"
     gene            complement(18561..19484)
                     /gene="rsgA"
                     /locus_tag="WGO49_000294"
     CDS             complement(18561..19484)
                     /gene="rsgA"
                     /locus_tag="WGO49_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ0305456.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSMRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENADLLRPVLQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETGAASPERLAFLQGITDELPG"
     gene            complement(19489..21345)
                     /locus_tag="WGO49_000295"
     CDS             complement(19489..21345)
                     /locus_tag="WGO49_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0305457.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALIFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPRALPLNVPQGAIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMNGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(21371..21508)
                     /locus_tag="WGO49_000296"
     CDS             complement(21371..21508)
                     /locus_tag="WGO49_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305458.1"
                     /translation="MPSEGGATFALTKPCVSASRCLPDGFSRKMALLSFLSDGIMFDA
                     V"
     gene            complement(21508..22092)
                     /gene="ruvA"
                     /locus_tag="WGO49_000297"
     CDS             complement(21508..22092)
                     /gene="ruvA"
                     /locus_tag="WGO49_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ0305459.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVSGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHAVTDGLFAAAPAADETEDIVGTLLA
                     LGYSEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(22174..22500)
                     /locus_tag="WGO49_000298"
     CDS             complement(22174..22500)
                     /locus_tag="WGO49_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0305460.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(22554..23273)
                     /locus_tag="WGO49_000299"
     CDS             complement(22554..23273)
                     /locus_tag="WGO49_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ0305461.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMVQTVLTAG"
     gene            complement(23270..23392)
                     /locus_tag="WGO49_000300"
     CDS             complement(23270..23392)
                     /locus_tag="WGO49_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0305462.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(23403..23867)
                     /gene="trmL"
                     /locus_tag="WGO49_000301"
     CDS             complement(23403..23867)
                     /gene="trmL"
                     /locus_tag="WGO49_000301"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ0305463.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(23932..24429)
                     /locus_tag="WGO49_000302"
     CDS             complement(23932..24429)
                     /locus_tag="WGO49_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0305464.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     SERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKSGANRICCWTLAR
                     TPMKK"
     gene            24485..25399
                     /gene="bioH"
                     /locus_tag="WGO49_000303"
     CDS             24485..25399
                     /gene="bioH"
                     /locus_tag="WGO49_000303"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ0305465.1"
                     /translation="MCLKHRGQLFAASVRYAAKSSRQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQRERKSMPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TAAEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            25401..26189
                     /locus_tag="WGO49_000304"
     CDS             25401..26189
                     /locus_tag="WGO49_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0305466.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWACALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
BASE COUNT         5682 a         6699 c         7395 g         6504 t
ORIGIN      
        1 cgaattacgg tgtaaaaatt tatatgacct tataaaatca aataagaatc gttatcataa
       61 catgattgta tttattgggt ttttttgggc gttttgccga tatttacctt ttaatggttt
      121 ttgaaattcg ctaaaatacg aaattattgt agaaattttg ttaacggatt tgggtgtaac
      181 catgttgtcc gcttactttc ccgtctttgt ctttatcctc gtcggcctcg cggccagcgt
      241 gctgtttatc ctgctcggca cgattttagg cccgaaacgc cactacgccg aaaaagacgc
      301 gccttacgaa tgcggttttg aagcctttga aaacgccagg atgaagttcg acgtgcgcta
      361 ttacctcgtc gccatcctct ttatcttgtt tgatttggag gtcgcgttca tgttgccgtg
      421 ggcagtcgtg ttcaaagatt tgggcgcgta cggcttctgg tctatgctgg tgtttatcgt
      481 cgttctgacg gtaggctttg tttacgaatg gaaaaaaggt gcgctggaat gggaatagaa
      541 ggcgttttga aaaaaggttt catcaccacc agcgcggata cggtgttgaa ctatatgcgt
      601 accggttcgt tgtggccggt tactttcggc ttggcctgct gcgccgtgga aatgatgcac
      661 gcgggtatgg cgcgttatga ccttgaccgt ttcggcatca ttttccgtcc gtccccacgt
      721 caggccgacc tgatgattgt ggcgggtacg ctgaccaaca aaatggcacc cgccctgcgc
      781 cgcgtgtatg accagctcgc cgagccgcgc tgggtattgt ctatgggctc gtgtgccaac
      841 ggcggcggct attatcacta ttcttattcc gttgtgcgcg gtgccgaccg cgtcgtgccg
      901 gtagacgttt atgtgccggg ttgtccgccg actgcggaag ccctgattta cggcctgatt
      961 cagctccaac aaaaaatcaa gcgcacttcc accatcgcgc gtgacgagta agaggaggaa
     1021 gatatggcaa gcattcaaaa cttatacgaa accgtcagcc gcgttttggg caatcaggca
     1081 ggcaaagtca tttcagcttt gggcgagatt accgtcgagt gtctgcccga acactatatt
     1141 tcagtcatga ccgcattgcg cgatcatgaa gagctgcatt tcgagcttct ggtcgatttg
     1201 tgcggtgtcg attacagcac ttacaaaaac gaagcatggc agggcaaacg ctttgccgtc
     1261 gtcagtcagc tgctttccgt taaaaacaat caacgcatcc gcgtacgcgt ctgggtttca
     1321 gacgacgact tccccgtagt tgaatccgta gtcgatattt acaacagcgc ggattggtac
     1381 gaacgcgaag ccttcgattt gtacggcatc atgttcaaca accacccgga cttgcgccgc
     1441 atcctgaccg attacggctt tgtcggacat ccgttccgca aagacttccc gatttccggc
     1501 tatgtggaaa tgcgttacga cgaagagcaa aaacgcgtga tttaccaacc cgttaccatc
     1561 gagccgcgcg agatcacgcc gcgtatcgtc cgtgaggaga actacggtgg ccaataaatt
     1621 aagaaactac accatcaact tcggcccgca acaccctgcg gcgcacggcg tattgcgtat
     1681 gattttggag ttggagggcg aaaccatcgt ccgtgccgat ccgcacatcg gcctcctgca
     1741 ccgaggtact gaaaaactgg cggaaaccaa aacctatctg caagccctgc cctatatgga
     1801 tcgcttggac tatgtttcca tgatggtcaa cgagcaggcg tattgcttgg cagtagaaaa
     1861 acttgccggt atcgatgtac ctatccgcgc ccaatatatc cgcgtgatgt ttgccgaagt
     1921 aacgcgcatc ctcaatcact tgatgggcat cggttcgcac gccttcgaca tcggcgcgat
     1981 gaccgccatc ctttacgcct tccgcgaccg cgaagagctg atggacttgt acgaagccgt
     2041 gtccggcgca cgtatgcacg cagcctactt ccgtcccggc ggcgtttacc gcgacctgcc
     2101 cgacttcatg cccaaatacg agagcagcaa attccgcaat gccaaagtat tgaagcagct
     2161 caacgaatcc cgtgaaggca ctatgctcga ctttatcgac gccttctgcg agcgtttccc
     2221 gaaaaacatc gacacactcg aaaccctctt gaccgacaac cgtatttgga aacagcgtac
     2281 cgtcggcatc ggcgtcgtct cccccgaacg tgccatgcaa aaaggcttta ccggcgtgat
     2341 gttgcgcggt tcgggcgtgg aatgggacgt gcgtaagaca cagccttacg aagtgtacga
     2401 caaaatggat ttcgacatcc ctgtcggcgt gaacggcgac tgctacgacc gctacctctg
     2461 ccgtatggaa gaaatgcgtc aatccgtacg catcatcaaa caatgttccg agtggttgcg
     2521 tgtcaatccg ggtccggtca ttaccacaaa ccacaaattc gctccgccca aacgtaccga
     2581 aatgaaaaca ggtatggaag acctgattca ccatttcaaa ctctttaccg agggtatgca
     2641 cgttcccgag ggcgagacct acaccgctgt cgaacatccg aaaggcgagt tcggcgttta
     2701 catcatttca gacggcgcaa acaaacccta ccgcctgaaa atccgcgcac ccggcttcgc
     2761 ccatctgcaa ggcatggacg aaatggcaaa aggccacatg ctcgccgacg tcgttgccat
     2821 catcggtacg caggacatcg tattcgggga ggttgaccga taatgttatc cgcagagtcc
     2881 ttaaaacaaa tcgacacaga actggcgaaa tatcccgccg accaacgccg ttccgccatc
     2941 atgggtgcgt tgcgtatcgc ccaaaccgaa aaaggctggc tcgcccccga aaccatcgct
     3001 tttgttgccg actatatcgg catcacgcct gcacaagcct acgaagtcgc cactttctac
     3061 aatatgtacg accttgagcc tgtcggcaaa tacaaactga ccgtttgtac caacctgccc
     3121 tgcgccctgc gcggcggtat ggctaccggc gaatacctca aacaaaaact cggtatcggc
     3181 tacggcgaaa ctacccctga cggcaagttt acccttgtcg aaggcgaatg catgggcgca
     3241 tgcggtgatg cccccgttat gctggtcaac aaccacagca tgtgcagctt tatgaccgaa
     3301 gaagcgattg agaagaaact ggcggagttg gagtaggtcg tctgaaacga cgatttaaac
     3361 gtaggtcgga tacttgtatc cgacagagtg ggtaaaaagg caaaatgtcg gatttaagaa
     3421 tccgccctac tgaaataccg aaatgccgtc attcccgcgc aggcgggaat ccaccggtaa
     3481 gattcggttt ctgaatttaa taagacattg cttaccattg aggatggatt ctcgcctgcg
     3541 cgggaatgac gacagacaag caagtggtcg agatccaaca aaaacgatta aaggtcgtct
     3601 gaaaatatcg atttgataaa ctagatttta tttcagacga cgttacaagc cggtacacaa
     3661 agacatctta aggtcgtctg aaacagcggc cgcaaccgat acgaaaacaa acaggcacac
     3721 caaaaatggc tatttaccaa tcaggcgtga tttttgacca agtggatacc gccaatcccg
     3781 attgctggac attggacgaa tacgtcaaac gtggcggtta taccgccctg cgtaaaatcc
     3841 tgtccgaaaa catctcgcaa accgatgtga ttgacgaagt caaaacctcc ggcttgcgcg
     3901 gtcgtggcgg tgcgggcttc ccgaccggtt tgaaatggag ctttatgccc cgttctttcc
     3961 ccggcgaaaa gtacgtcgtt tgcaacaccg acgaaggcga gccgggtaca tttaaagacc
     4021 gcgacattat tatgttcaac ccccatgccc tgattgaagg catgattatc gccggttacg
     4081 cgatgggcgc gaaagccggt tacaactata tccacggcga aatttttgaa ggctaccaac
     4141 gctttgaggc cgctttggag caggcgcgtg ccgcaggctt tttgggtaaa aatattttgg
     4201 gttcggattt tgaattcgaa ctcttcgccc accacggcta cggtgcatac atctgcggcg
     4261 aagaaaccgc attgcttgaa tcgctggaag gcaaaaaagg ccagccgcgc tttaaaccgc
     4321 cattccctgc ttcgttcggc ctgtacggca aaccgactac catcaacaat accgaaactt
     4381 tctcctccgt ttcattcatt atccgtgacg gcggacaggc atttgccgat aaaggtattc
     4441 cgaatgcagg cggtaccaaa ttattctgta tttccggcca tgtcgagcgt ccgggcaact
     4501 atgaagtgcc attgggcaca ccgtttgccg aagtcttgaa aatggcaggc ggtatgcgcg
     4561 gcggcaaaaa actcaaagcc gtcattcccg gcggttcgtc cgcgcccgta ttgcctgccg
     4621 acatcatgat gcagacaaat atggactacg actcgatttc caaagcaggc tcgatgctgg
     4681 gttctggcgc gattatcgtc atggacgaag acgtgtgcat ggtgaaagcc cttgagcgcc
     4741 tgagctactt ctactacgac gagtcttgcg gccaatgtac gccttgccga gaaggcaccg
     4801 gctggcttta ccgcatcgtc caccgcatcg tagaaggcaa aggtaaaatg gaagatttgg
     4861 atttgctgga ttctgtcggc aaccaaatgg caggccgcac catctgcgcc cttgccgatg
     4921 ccgccgtctt ccccgtccga agctttacca agcatttccg tgatgagttt gtgcattaca
     4981 tcgaacacgg cggaccgatg aaagagcata agtggggagg gtggtaatgg tggaagctaa
     5041 aatttttatt ctatacggtg cagccaacaa aggtaagagt acgacactca atacgctttt
     5101 taatcagatt tgtcggaaat tttctaaatt tctagtcttt tttgaaagac atggaaacgg
     5161 cttagatttt gttgcagtat ttgatcatga aggtcagagg attggttttt attcatctgg
     5221 tgataatgaa tacgaggtta ggagaaattt atacaaactt tattcgcata attgtgattt
     5281 tatttttggc acgtcaagga cacggggtgg tagttgcgat gcagtaggat gttatgcaga
     5341 gttattgcat ggcgatgtaa atataattaa ttggtgtgaa aagtttgagc ctacagatga
     5401 agacaatgag cgtgctgtta aagagttatt taagtcattt aaaaatataa taaataagtt
     5461 atagttttag ttggttttat attggttaaa agcaaaatgc taaaaattta actttgccgt
     5521 cattcccgcg taggcgggaa tccatagtgg aatttacaga acccgatatt tgaaaagcag
     5581 ttaccgaaaa cccaagaatg gattcccgcc tacgcgggaa tgacggcaag gtggcagtaa
     5641 acgttttaaa cagtattgat tgtcaatgaa actcaaaagg ccgtctgaaa cccatttttc
     5701 agacgatctc cataaaaaat tatttatcaa atacccgtaa ctaggaacga accatgttac
     5761 aaatcgaaat cgacggcaaa caagtatctg tggagcaggg cgcgacggtg attgaagccg
     5821 cgcacaagct cggtacttat attccgcatt tctgttacca caaaaaactt tccatcgccg
     5881 ccaactgccg tatgtgtctg gtgaacgtag aaaaagcccc aaaacccctg cctgcctgtg
     5941 ccacgccggt tacagacggc atgattgtgc gtacgcattc ggcaaaagcc cgagaggcgc
     6001 aggaaggcgt gatggagttc ctgctcatca accatccgct tgattgtccg acctgcgacc
     6061 aaggcgggga atgccagttg caggatttgg cggtgggcta cggaaaaacc accagccgct
     6121 acaccgaaga aaaacgttcc gtcgtcggca aagacatggg gcctttggtt tccgccgagg
     6181 aaatgagccg ctgtatccac tgcacccgct gcgttcgttt cactgaagaa atcgccggtt
     6241 tgcaagaaat tgcgatggtg aatcgcggcg aacactccga aatcatgccc tttatcggca
     6301 aaacggtgga aaccgagctg tcgggcaacg tcatcgattt gtgtcctgtc ggcgcattga
     6361 ccagcaaacc gttccgcttc aacgcgcgta cttgggaatt gaaccgccgc aaatccgttt
     6421 ccgcccatga cgctttgggc agcaacctga ttatacaaac caaagaccat accgtccgcc
     6481 gcgtgttgcc gctggaaaac gaagcgatta acgaatgttg gctgtctgac cgcgaccgtt
     6541 tcgcctacga aggcctgtat cacgaaagcc gtctgaaaaa cccgaaaatc aaacagggcg
     6601 gcgagtggat ggacgtggat tggaaaaccg cgttggaata tgtccgcagc gcgattgaat
     6661 gtatcgccaa agacggcaac caaaaccaag tcggcatttg ggccaatccg atgaatacgg
     6721 ttgaagagct gtatctggcc aaaaagcttg ccaatggttt gggtgttaaa aactttgcca
     6781 cccgtttgcg ccaacaagac aaacgtcttt cagacgacct taaaggtgca caatggttgg
     6841 gacaaagcat tgaatctttg gctgacaacg atgccgtatt ggtagtcggc gcgaacttgc
     6901 gcaaagaaca gccgctcctg accgcccgcc tgcgccgcgc cgccaaagac cgtatggcct
     6961 tgagcgtatt ggccggcagt aaagaagaat tgtttatgcc gcttctgtct caagaagccg
     7021 cacatcccga cgaatgggca ggccgtctga aaaaactgtc tggcaatgcg gaacacgcag
     7081 ttaccgccag cctgaaaaac gctgaaaaag cagcggtgat tttgggtgcg gaagtgcaaa
     7141 accatcctga ttacgccgcg atttatgccg ccgctcaaga gctggcagat gcgaccggcg
     7201 cagtgctggg tattttgccg caagccgcca acagcgttgg tgcggatgtc ttgggcgtga
     7261 actcgggcga gagcgttgcc gaaatggcaa acgcgccgaa acaggcggtc ttgctactca
     7321 acgtcgaacc ggaaatcgat acggcggacg gtgcgaaagc cgtggccgcg ttgaaacagg
     7381 cgaaaagcgt gatggcgttt acaccgtttg tcagcgaaac gctgctggat gtgtgcgacg
     7441 tgttgttgcc gattgcgccg tttaccgaaa catcgggcag cttcatcaat atggaaggcc
     7501 gtctgcaatc ctttcacggc gtggtacaag gcttcggcga ctcgcgtccg ctgtggaaag
     7561 tgttgcgcgt attgggcaac ctgtttgacc tgcaaggttt tgaataccac gataccgccg
     7621 cgattctgaa agacgcgctg gacgcggaaa gcctgccgtc caaactggac aaccgcgtgt
     7681 cttcgactca aaaagatttt cagacggcat caagccgcct cgtgcgcgtg ggcggagtgg
     7741 gcatctatca caccgatgcc atcgtgcgcc gttccgcacc gttgcaggca accgtccatg
     7801 ccgccgtgcc tgccgcgcgt gtgaatccaa acacattggc acgcttgagc ctgcaagacg
     7861 gacaaattgc tgtcgccaaa caaaacggcg caagcgtatc ggttgctgtc aaagccgatg
     7921 ccggtttgcc tgaaaacgtg gtgcatctgc cgctgcatac cgaaaatgcc gcgctgggtg
     7981 cgttgatgga cactattgaa ctggcgggag cttgatcatg caagaatggt tccaaaacct
     8041 ctttgccgca acgctcggtc tgggcgattt gggtatcacc gtgggcttgg tggtatccgt
     8101 catcgtcaaa atcgtgatta tcctgattcc gctgattctg accgtcgcct acctgactta
     8161 tttcgaacgt aaagtcatcg gcttcatgca gcttcgcgtc ggcccgaacg taaccggccc
     8221 gtggggcctg atccagccgt ttgccgatgt gttcaaactc ttgtttaaag aggtaacccg
     8281 tccgaagctg tcaaacaaag ccctgttcta catcggcccg attatgtcgc ttgctccgtc
     8341 tttcgcggca tgggcagtga ttccgttcaa cgaagaatgg gtgctgacca acatcaatat
     8401 cggtcttttg tacattctga tgattacctc gctgtcggtt tacggcgtaa tcatcgcggg
     8461 ctgggcttcc aactccaaat attcgttctt gggtgcaatg cgtgcttccg cacaaagcat
     8521 ttcctacgag attgccatga gtgccgcgct ggtgtgtgtc gtgatggtgt cgggcagcat
     8581 gaacttctcc gacatcgttg ccgcgcaggc aaaaggtatc gcgggcggtt ccgtattctc
     8641 gtggaactgg ctgccgctgt tcccaatctt catcgtctat ctgatttccg ccgttgccga
     8701 aaccaaccgc gcaccgtttg acgtggcaga gggcgagtcc gaaatcgttg ccggacacca
     8761 cgttgaatat tccggcttcg cattcgcgct gttcttcctt gccgaataca ttttcatgat
     8821 tctgattgcc gcgctgacat cgctgatgtt tctcggcggc tggttgtctc cgttcccgca
     8881 aagctggggc tttatcggta cgccttccgc attctggatg tttgtgaaaa tggcggcagt
     8941 gttgtactgg tacctgtgga tccgtgcaac cttcccgcgc taccgttacg accaaatcat
     9001 gcgtttgggc tggaaagtgc tgattccgat cggcttcgcc tacatcgtga ttttgggcgt
     9061 gtggatgatt tcaccgctga atttgtggaa ataagtttca gacggcatct tgaggccgtc
     9121 tgaacaaagc gattttgaat acctaacgaa atccctgttt tgagggaaca taatatggct
     9181 aacttagtaa aaacctttct gctcggcgag ttggtaaaag gcatgggcgt aacgctcaaa
     9241 aacttttttg cccgcaaaga cacaatttat ttccccgaag agaaaacgcc gcaatccgtg
     9301 cgtttccgtg gcctgcacgc gcagcggcgg tatccgaacg gcgaagagcg ctgtatcgcg
     9361 tgtaaattgt gcgaggcagt ttgtccggca atggcgatta acattgaatc ggaagaacgc
     9421 gaagacggca cccgccgcac caagcgttac gatatcgacc tgaccaagtg catcttctgc
     9481 ggtttctgcg aagaggcctg cccgaccgat gcgattgtgg aaacgcatat ttttgaatac
     9541 cacggcgaga aaaaaggcga cttgcacatg accaagccga tcctcttggc aatcggcgac
     9601 aaatacgaag ctgaaatcgc caaacgcaaa gccgctgatg cgccgtatcg ttaaggagca
     9661 gacgaatgac tttttccgcg attctgttct atatccttgc cgccatcgtc ctatacggcg
     9721 cgctgcgtac cgttacagcg aaaaaccctg tccacgccgc tttgcatctg gtgttgacct
     9781 tctgcgtgag cgcgatgctt tggatgctga tgcaggctga gtttttgggc gtgacgctgg
     9841 tggtggttta cgtcggcgcc gtgatggtgt tgttcctgtt cgtcgtgatg atgttgaaca
     9901 tcgacattga agaaatgcgt gccggtttct ggcggcacgc gcctgttgcc ggtgtggtcg
     9961 gcacattgtt ggcagttgcc ctgattctga ttctggtcaa tccgaaaacc gaccttgccg
    10021 catttgggct gatgaaagac attcccgccg attacaacaa tatccgcgat ttgggcagcc
    10081 gtatttatac cgattatctg ctgccgtttg aattggcggc ggtattgctg ctgttgggta
    10141 tggtggccgc gattgcgctg gttcaccgta aaacggttaa tccgaaacgt atggaacctg
    10201 ccgaccaagt taaagtacgc gccgaccaag gccgtatgcg cttggtgaaa atggaagccg
    10261 tgaaaccgca aaccgaatct gccgaagaaa gcgaagtttc agacgacctc aagccgaaag
    10321 aggagggcaa agcatgatta ccttgacgca ttatctggta ttgggtgcgc tcctgttcgg
    10381 tatcagcgcg atgggtatct tcatgaaccg caaaaacgtg ctggtattgt tgatgtccat
    10441 tgagctgatg cttttggcgg tgaacttcaa ctttatcgcc ttttcgcaac atttgggcga
    10501 tactgccgga caaattttcg tatttttcgt actgaccgtt gctgctgccg aatctgccat
    10561 cggtttggca attatggtgc tggtgtaccg caaccgacaa acgattaacg ttgccgattt
    10621 ggacgagttg aaagggtaaa ggtaggttgg gtcgagacct gacaagacac cgatgccgtc
    10681 tgaaaacccg ataggaaaaa cgatgaaatc catagacgaa caaagcctgc ataatgcccg
    10741 ccgcctgttt gaaagcggcg acatcgaccg tatcgaagtc ggtaccaccg cgggcctgca
    10801 acagattcac cgttacctgt tcggcggctt atatgatttt gcgggtcaaa tcagggaaga
    10861 caacatttcc aaaggcggtt ttcgttttgc caacgccatg tatttaaaag aggctttggt
    10921 taaaatcgag cagatgcccg agcggacttt tgaagaaatc atcgccaaat atgttgaaat
    10981 gaacattgcc catccgtttt tggagggtaa tggcagaagt acccgcatct ggctggattt
    11041 ggtgctgaaa aaaaacctga aaaaagtcgt gaactggcaa aatgtaagta aaaccctgta
    11101 tttgcaggca atggaacgca gccccgtcaa cgatttagaa ctgcgctttc tgttaaagga
    11161 caacctgact gacgatgtgg acaaccgtga aatcatcttt aaaggtatcg agcagtcgta
    11221 ttattacgaa gggtatgaaa aaggctgagg gtcgtctgaa aagcgatttc agactgtttc
    11281 agacgacctg attcggtagg tgatcagacg ggagcggatg agaaaagaaa ttctgggtaa
    11341 gaataatccg gtctgaaata ttggaagaag aatgatggat aaaaatcagt tagaacaaga
    11401 atttcataaa gccatgttaa atatttatca ggaggctttg aatttgccgc aaccttacaa
    11461 ggcgacacga tttttacaaa ttgtaaatga atttggtggt aaagaggcgg cggataaatt
    11521 attaagtacg ggggaaaaga agactcagac cggttttaca gagctgattt tgagtggtgg
    11581 cggagtccac gccttgaaat acagtatgga atatctggtg ttacaaaagc cgtggtgtga
    11641 tttatttact gaagagcaat tagctgtggc acgcaaacga ttggagcgtg ttggatttgt
    11701 ttctccgaag taattttgta cgaaacaaac atagattttt aaatcaatcg gattcaatca
    11761 aatgaacgac atgactttat atctcatcat tgcccttgtg cctttggcag gctcgctgat
    11821 cgcgggtttg ttcggcaaca aaatcggacg tgccggtgcg catacggtta cgatactcgg
    11881 cgtggcggtg tccgccgtgc tgtcggctta tgtgttgtgg ggctttattg acggcagccg
    11941 cgccaagttt gacgaaaacg tctatacctg gctgacaatg ggcggcttgg atttctccgt
    12001 cggctttttg gtcgatacga tgacggcgat gatgatggtc gtggtcaccg gcgtatcgct
    12061 gatggtacat atctatacca tcggttatat gcacgatgaa aaaatcggct accaacgctt
    12121 cttcagctat atttctttgt ttactttcag catgttgatg ctgattatga gcaacaactt
    12181 catccagctc ttcttcggtt gggaagcggt gggcttggtg tcgtatctct tgatcggttt
    12241 ctatttcaaa cgtccgagcg cgacatttgc caacctgaaa gcctttttga tcaaccgtgt
    12301 cggcgacttc ggctttttgc tcggtatcgg cttggtgctt gcctatttcg gcggcagcct
    12361 gcgctatcaa gatgtattcg cttatctgcc taatgttcag acggccacca ttcaattgtt
    12421 ccccagtgtg gaatggtctt tgattactgt aacctgtttg ctcctgtttg tcggtgcgat
    12481 gggtaaatcg gcacaattcc cgctgcacgt ctggctgcct gattcgatgg aaggcccgac
    12541 cccgatttct gcattgattc acgccgcaac catggttacc gccggtctgt ttatggtatc
    12601 gcgtatgtcg ccgatttatg agatgagtag cactgcgctg tctgtcatca tgatgattgg
    12661 tgcgattacc gccctgttta tgggtttctt gggcgtgatt caaaacgata tcaagcgcgt
    12721 ggttgcgtat tccaccctgt cgcaactggg ctatatgacc gtggctttgg gcgcgtccgc
    12781 ctattccgtg gcgatgttcc acgtcatgac ccacgccttc tttaaagcct tgctgttctt
    12841 ggcggcaggt agcgcgatta tcggtatgca ccacgaccaa gatatgcgcc atatgggcaa
    12901 cctgaaaaaa tatatgccgg ttacttggct gactatgctg atcggcaatt tgtcgctgat
    12961 cggtacgccg ttcttctccg gcttctattc taaagattcg attatcgaag cagcgaaata
    13021 cagcaccctg ccgggcagcg gctttgctta ttttgccgtc cttgccagcg tgttcgttac
    13081 cgcgttttac gcgttccgcc aatactttat ggtgttccac ggcgaagaga aatggcgcag
    13141 cctgcctgaa catcatgacg atcatcatgg tgaagaacat cacggcttgg gcaaaaacga
    13201 caatccgcac gaaagcccgc tggttgttac cctgccgctg attttgcttg ccatcccgtc
    13261 cgtcatcatc ggctatgttg ccatcgagcc tatgctttac ggcgatttct ttaaagacgt
    13321 gattttcgtc aataccgacg cgcatccgac catgcacatc atgaaggaag agttccacgg
    13381 cgcattggca atggtgtcgc acagcctgca ttcgcctgta ctctaccttg ctattgcagg
    13441 tgtcttaagc gcatggcttt tgtacgtcaa actgccgcac ctgcctgcga aaattgcaca
    13501 ggcgttccgt ccgatttacg ttttgtttga aagcaaatac tacctcgacg ccctgtattt
    13561 caacgttttc gccaaaggca cacgcgcatt gggtaccttc ttctggaaag tcggcgatac
    13621 cgccattatc gacaacggca tcgtcaacgg ctctgccaaa ctggtcggcg taattgccgc
    13681 gcaagtgcgt aaagcccaaa ccggctttat ctacacctac gccgccgcta tggtattcgg
    13741 cgtattggta ctgctcggca tgaccttctg gggattgttc agataaggat gaggtttcag
    13801 acggtctctt aaaacgtcgt ctgacaaacc aacccgtaca acgatatttt tattttaacc
    13861 acaggttaac cactatgttt tccaactacc tactcagctt ggcaatatgg atacccatcg
    13921 ccgcaggtgt gctggttttg tcgaccggta aggacagccg tgcgccgctg gcgcgtgtgc
    13981 tcgccttcat gggtgcgctt gccggtttct tggtaacgct gcccctgttt accggtttcg
    14041 accgtttgag cggcggctat caatttaccg agttccacga gtggattccg ctgctgaaaa
    14101 tcaactatgc attgggcgtg gacggtattt cagtgctctt tatcatcttg aatgcgttta
    14161 ttacgctgtt ggtggtgctg gcgggttggg aagttattca gaaacgcccg gcgcagtata
    14221 tggcggcatt cctgatgatg tcgggtttga ttaacggtgc gtttgccgcg caggatgcga
    14281 ttctgtttta tgtgttcttc gagggtatgc tgattccgct gtacctgatt atcggtgtat
    14341 ggggtggccc gcgccgcgtt tatgcgtcgg tcaagctgtt cctctatacg ctgatgggtt
    14401 cgcttttgat gctggttgcc atggtttacc tgtactatca gacaggcagc ttctctattg
    14461 tcgatttcca aaacatcaag cagattccat tgggcgtaca acagcttttg tttgtggcat
    14521 tcttcctgtc atttgccgta aaagtgccga tgttccctgt gcacacttgg ttgccggatg
    14581 cccacgttga agcgccgacc ggcggttcga tggtgttggc ggcaattacg ctgaaactgg
    14641 gtgcgtatgg tttcttgcgt tttatcctgc cgattatgcc ggatgcggca cgctattttg
    14701 cccctgtgat catcgtattg agcctgattg ccgtgattta tatcggtatg gtggctttgg
    14761 tgcaaaccga tatgaaaaaa ctggtggcgt attcgtctat cagccatatg ggttttgtaa
    14821 cgcttgggat gtttttgttt gttgacgggc agttggacga ctgggcgttg aaaggcgcaa
    14881 tcattcaaat gatttcgcac ggtttcgtgt cggcggcgat gtttatgtgt atcggcgtga
    14941 tgtacgaccg cctgcatacc cgcaatattg ctgactatgg cggcgtggtc aatgtgatgc
    15001 ctaagtttgc ggcgtttatg atgttgttcg gtatggccaa tgccggtctg cctgcaactt
    15061 ccggcttcgt gggcgagttt atggtgatta tgggcgcggt taaagtgaat ttctgggtgg
    15121 gcgcgttggc ggccatgacc ctgatttacg gtgcgtctta taccctgtgg atgtacaagc
    15181 gcgtgatttt cggcgcgatc cacaatccgc acgttgccga aatgaaagac atcaactgcc
    15241 gcgaatttgc gattctggcg attttggcgg ttgtggtatt gggtatgggt ctgtatccga
    15301 acgcatttat cgaagtggtg catcaggcgg caaacgattt gattgcccat gtggcacaaa
    15361 gcaagatttg aggtgtgtaa atgaactggt ctgatttgaa tttgatgccc gccatgcccg
    15421 aaatcgtgct gcttgcgctg ctggtgttat tgttgctggc ggacttgtgg gtcagtgatg
    15481 acaaacgccg gtggacgcat tacggcgcgt tggcaacggt ggcggttacg gctgtggtgc
    15541 agcttgccgt ttgggaacag ggcagcacgt cttcgttcaa cggcatgtat attgcagacg
    15601 gtatgtcgcg tttggcaaaa atggttttat atgccttgac gtttgccctg tttgtttatg
    15661 ccaagcccta caaccaagtg cgcggtattt tcaaaggcga gttttacacc ctgtcgctgt
    15721 ttgccctctt gggcatgagc gtgatggtga gcgcggggca ttttttaact gcctatatcg
    15781 gtttggaact cttgtcgctc gccctttacg ccctgattgc cctgcgccgc gattccggct
    15841 ttgccgccga agccgccttg aaatattttg ttctgggcgc gctggcatcc ggcctgctgc
    15901 tctacggtat ttctatggtt tacggcgcaa ccggttcgct ggaatttgcc ggcgtgctcg
    15961 cctcttcctt caatgaagaa gcaaacgaat ggctgttgaa actgggtctg gtgtttatcg
    16021 tcgtcgccgt tgcgttcaaa ctcggcgcgg tgccgttcca tatgtgggtg cccgacgtgt
    16081 atcacggcgc gcccacttct gttaccgccc tagtcggcac tgccccgaaa gtcgccgccg
    16141 tcgttttcgc tttccgcatc ctcgttaccg ggttgggaac cgtgcatcat gactggtctc
    16201 tgatgtttgc cctgcttgcc gccgcctcgc tgctggtcgg caaccttgcc gccatcatgc
    16261 agaccaatat caaacgtatg ctcgcctatt ccaccgtatc gcatatgggc tttatcttgt
    16321 tggcgtttat ggcaggcgcg gtcggctttg cggcgggtct ctattacgcc attacctacg
    16381 cgctgatggc ggcggcaggg ttcggcgtgt tgatggtgtt gtcggatggg gacaacgagt
    16441 gcgaaaacat cagcgatttg gcagggctga accaacaccg cgtatggctt gcctttttga
    16501 tgctgctggt tatgttctcc atggcaggca ttccgccgct gatgggtttt tacgccaaat
    16561 tcggcgtgat tatggcactc ttgaaacaag gctatgtttg gttgtccgta tttgccgtcg
    16621 tgatgtcgct gattggtgcg ttctactacc tgcgcgtggt caaagtcatg tattttgacg
    16681 aatccggccg cgcccatccc accgtcggcg gcaacaatgc cgcaaaattc cttttgagcg
    16741 tcaacgcgct gctgttggtg ctgtggggca ttatgccgca aaccgttatc gactggtgcg
    16801 ccaaggcgtt ggaaaacacg ctgtaagccg ccgcaacggc agccgtgtca gaggctgccg
    16861 tttttgttaa gatatgccgt tccgcaatgc agttcagacg gcatcgccgc cgacaatgcc
    16921 caaacagaaa gcccgccatg accgcatcca tgtacatcct tttgctgctt gccttgattt
    16981 ttgccaacgc ccccttcctc acgaccaggc tgttcggcat cgtaccgctc aagcgcaaac
    17041 atttcgggca ccacttgatc gagctggcgg caggtttcgc gctgaccgcc gttcttgcct
    17101 acatcctcga atcccgtgcg ggagcggtac acgatcaggg ttgggagttt tacgccaccg
    17161 tcgtctgcct gtacctgatt tttgcgtttc cctgtttcgt gtggcggtat ttttggcaca
    17221 cgcgcaacag ggaataggca ggcataggaa tgccgtctga aacctgttca gacggcattt
    17281 gtttcattca agtgcaggcc ggcatcgctg tgccggcacg tttcagccgg cgatatacgc
    17341 cggttttaat atttgcgggc gactgcaaat tctgccaact gccgcaggcg cagggctttg
    17401 tcgccgaagg gttcgagcag cgcgaccgct tcggcaacca gtttgtgtgc gtatgagcgc
    17461 gccgcttcca agcccatcag tttcacataa gtcggcttgt cgttgtctgc gtctttgccc
    17521 gccgttttgc ccaaagtcgc cgtgtccgct tcacaatcca acacatcgtc aatgacttgg
    17581 aacgccagcc ccagttttgc cgcgtaagcg tccaatacgg aaagttccgc atctgacaga
    17641 tcaggacacg ccgtcgcccc caataaaacc gccgcacgga ttagcgcacc cgttttcagg
    17701 ctgtgcatct gttccaaatc ggcttgaacc atttgtttgc cgacattcgc caaatcgatt
    17761 gcctgaccgc ccgccatacc cctgctgccg cccgctttcg ccaacaccga caacattgcc
    17821 aactggcgtg cggcgggcag ttctgtcgga cgacttaaca cgtcgaaggc ttgggtttgc
    17881 aaagcatcgc cggtcagcag tgcgtttgct tcgccatatt tgatgtggca ggtcggtttg
    17941 ccccggcgca ggctgtcgtt gtccatcgcc ggcatatcgt cgtgaaccaa agaatagacg
    18001 tggatcattt cgattgccgc cattgcctgt tctactgctt catgcacggc ttcgcctaat
    18061 tccgaagctg ccagaaccag catcggacgc agacgcttgc cgccgtccaa agccgcatag
    18121 cgcatcgctt cgtgcagcgt gtgcgggatt tcgttttcag acggcaaaaa gcgttccagc
    18181 agcagctctg tctgtgcctg cgccctctgt tgccacgctt tcaaatcatt cgtcggattc
    18241 aaggttcaac tccttcggcc cgtccgcatc caaaacctgt aatttctgtt cgacttgtgc
    18301 cagcttggtt tggcagtacc tgaccagctc gttgccttcc tgataggcgg caagcgcgtc
    18361 ttccaagggc atttcaccct gcatagactg cgtcagcgat tcaaggcgcg acaaggcttc
    18421 ttcaaacgat ttcggggtaa tttttttcat ggtgtcggga gtttctgtaa aaacggaatt
    18481 gtagcatttc tgaaaaccgg atgccgtctg aaacagcagg atttcagacg gcattgtttt
    18541 gtcctgcaaa cggcaaggcg ttacccgggc agttcgtcgg tgatgccctg caaaaaggcg
    18601 aggcgttcgg ggcttgccgc cccggtttcg gcggcggctt tgaaggcgca gccgggttcg
    18661 gcgcggtggg tgcagttgtg gaagcggcat tgcccgacaa ggtggcggaa atcggggaaa
    18721 tagcgcggca aatcggcggc ttggaggtgg tgtaaaccaa attcttgcaa acccggggag
    18781 tcgatgagtt gggtttcgcc gttcaaatca taaagccggg cgtgggtggt ggtgtgtttt
    18841 cccgagtcga gtgcggcgga aatgtcgccg gtgcgggcgg tttggctgcc taaaagggcg
    18901 ttggtcaggg tggatttgcc cataccgctc tgcccgagca ggatgttgct gtgcccttgc
    18961 aggacggggc gcaggaggtc ggcgttttcc agcgcacggg tttcgatgac gggataaccc
    19021 agcgtttcgt agaatttgag tttttcgcgc caaagggcgg tttcgggcag gtcggctttg
    19081 ttcaggacga tgacggcttc aataccggcg gcttcggcgg caagcagggc gcgttgcagc
    19141 agccgcatgc tcggactcgg gacggcggcg gtaacgataa ggagttgggt aacgttggcg
    19201 gcgatgagtt tggttttcca cgcgtcttgg cggtagagca ggctttggcg cggtaaaaaa
    19261 tcttcaatca caacttgttc ggcgttgacg gggctgatgc ggacacggtc gccgcaggcg
    19321 aaatcgacgc gttttttgcg cgtgctggct tcgtaggttg tgccgtcggg cgtgcgcacg
    19381 atgtagcggc ggccgtagct ggcggtaatt tgggcggtgt cgttcatggt ttctttgggg
    19441 ttgggtgtgg aaatgccgtc tgaaaacggg tgttcggacg gcatcggttc agtcgtgctg
    19501 ccactcgacg tgttcgttga ggaagccgcc gctctggtgc gcccagagtt tggcgtaaag
    19561 cccgcgtttt tcgaggagtt cggcgtgtgt gccttcttcg atgatgcggc ctttgtcgag
    19621 gacgacgagc ctgtccattg cggcgatggt ggaaaggcgg tgggcgatgg cgatgacggt
    19681 tttgccgttc atcattttgt cgaggctttc ttggatggcg gcttcgactt cggaatcgag
    19741 cgcgctggtg gcttcgtcca aaagaagaat cggtgcgtct ttgagcatca cgcgggcgat
    19801 ggcgatacgc tggcgttgac cgccggagag tttcacgccg cgttcgccga cgtgcgcgtc
    19861 atagccgcgc cgccctttgg catcggaaag ttcggggatg aagccggcgg cttcggcgcg
    19921 ttcggcggcg gaaaccattt cggcatcggt cgcgtcgggg cggccgtaaa taatgttgtc
    19981 gcgcacggaa cggtgcagca gcgaggtatc ttgcgtgacc aaaccgattt gggcgcgtaa
    20041 agattcttgg gtaacgccgc ttatgtcctg cccgtcgatc gaaaccgtgc cgctttgcgg
    20101 ttcgtagaag cgcaaaagca ggttgacgat ggtggatttg cccgcgccgc tgcgtccgat
    20161 caagccgact ttttcgcccg ggcggatggt gaggttgaag ccgttgagca gcggtttgcc
    20221 ggcttcgtag cagaaatcga tgtgttcaaa tttgattgcg ccttgcggca cgttcagcgg
    20281 cagtgcccgg ggcttgtcga ggatggtgtg cggtttggac agggttgcca tgccgtcgcc
    20341 gacggtgccg atgttttcaa acagccgcgc ggattcccac ataatgtatt gcgacaaacc
    20401 gttgacgcgc aacgccatgg cggtggctgt agcaaccgcg cccacgccga cctgcccgtt
    20461 gtgccagagc cagatgccca gtgcggcggt ggagagggtc agggaggtgt tgacgatgaa
    20521 gctgcacgaa tgcagcagcg tcgccagccg catttgggcg cgcaccgtaa ccataaattc
    20581 ttccatcgac tgcttggcat aggcggcttc acgcgcgccg tgggagaaga gtttgacggt
    20641 ggcgatattg gaataggcat cggtaatgcg gccggtcatc agcgagcggg catccgcctg
    20701 ccatgcggcg gtttgcccca atttgggaat cagcaggcgc atcaccgaag cgaaaccgac
    20761 aatccagccg ataaagggca gcagcagcca tgagtcgagc gaggcgagaa tcacgccgga
    20821 ggtaatgaaa tacaccgaca cataaacgac catatcggca accgtcatca ccgcgtcgcg
    20881 caacgccagc gcggtctgca tgactttggc ggacacgcgt ccggcaaatt cgtcctgata
    20941 aaaaccgagg ctttggttca gcatcaggcg gtggaagttc cagcgcaggc gcatggggaa
    21001 cacgccctga agggtttgca ggcgcacgtt ggacgcggca aacgcccaca cgaccgaaaa
    21061 caccatcatc gccgccattg ccgccagttc ccaacctttt tcggcaaaca attcggcggg
    21121 cgcgtatttg ccgagccact ccacgatttt gcccataaat tgaaaaatca gggcttccat
    21181 aatgccgatg ccggcggtca gcgcagccag ggcggctatc catttccgca cgccggccat
    21241 gctgctccag acaaaccgcc acaagccttt ttctggcgtt ttcggggcgg cttcgggata
    21301 agggtcgatt cgggactcga accaggaaaa gattttgttc aacattgttt tcgatttcgg
    21361 taaaacagtt tcagacggca tcaaacataa tgccgtctga aaggaaggac aataacgcca
    21421 ttttacggga aaagccgtcg ggaagacagc gcgaggcgga aacgcagggt ttcgtcaggg
    21481 caaacgtcgc gccgccttca gacggcatta tttcagcagg tttttcaaag caaggcgcac
    21541 gccttcgccc acgtccgtcc ctttcggaac gcctttgacc gccgcttttg cctcgcgttc
    21601 gctgtaaccg agggcgagga gcgtgccgac gatgtcttcc gtttcgtcgg cggcgggtgc
    21661 ggcggcaaac agcccgtccg ttaccgcatg cgcgaccagc ttgccgcgca gttccaaaac
    21721 catgcgttcg gcggtttttt tgccgattcc cggggcggag gagaggcgtt tgacatcttc
    21781 ttctgcaacc gcctgcgcca gctcgtctgc cgtcatcgcc gacaaaatgc ccaaagccgt
    21841 tttcgcgccg atgccgctga ccttaatcag ttggcggaat gttttgcgtt cttccgcagt
    21901 ggcaaaacca aataaaagat gtgcgtcttc ccgaatgata agctgggtaa acagttgtac
    21961 gctttcaccc acgggcggca ggttgtagaa ggtctgcatc gatacgtcgg cctcatagcc
    22021 gacaccgttg acatcgatga cgatttgcgg agggtttttt tcaaccagtt tgccggtcag
    22081 tctgctgatc atgtatgccg aatcctgaag tgtcgggtgc aaaatgccgt ctgaaaccgg
    22141 ttcgggcttc agacggcacg gattgtatca aattcagtcg tcgcggcggg aggaaatcac
    22201 gcggccggta cgggcatcga caacgacttt gtattcctgt ccgtttttga cgatttcgac
    22261 atcatagtgc ggacggccgt tgtcgtgttc gagatcgatg tcggtgattt tgccgccgac
    22321 acgcgccaac gctgcttttt cggcttgggc gcggctgatg attttgtctt gtttgctgtg
    22381 ttggtgtgcg gcgtgtccgt ggtcgtcatc gccgtgtccg tcgtggtggg cgagtgcggg
    22441 ggcggaaatg cccatcagtg cggttgcggc ggcggtcaag agaaggcgtt tgaagttcat
    22501 gttttgctcc tgcgaagtat ctggtggtgt ttgaaagacg taaaggcggg acatcaaccg
    22561 gcggtcaata ccgtctgaac cataccgcgc gcctgagctt cggcctcggc ggcgcgttcc
    22621 tgcgaggcaa acggacccat tttgacgacg tattcgtaac ggcgtttttc aatggcgagg
    22681 ttcgggcttg atgtcgaagc ggcgaagttt tgggcggctt ggttcagata ggcttgtgct
    22741 tcgtgttccg taccgaaaga tttcaagtcg ataaagatgt ctttgttttc ggcaaccggt
    22801 gcggatttgc ccgggacgat ttgttcgatt ttgacgtgcg ccgtcccttg gctgacaaag
    22861 cccaattttt gcgcggcggc tttggatacg tcgatgatgc ggttgccgtg gaaggggccg
    22921 cggtcgttga cgcggacgat gacgcttttg ccgtttttgg tattggttac gcgcacatag
    22981 ctggggatgg gcagggtttt gtgggcggcg gtaaaggcgt tcatatcgta tcgttctccg
    23041 ccggaagttt tgcgcccgtg aaacctgccg ccgtaccacg aggcgttgcc ggtttgcgtg
    23101 aattcggcga cttggttttt cggcgtgtag cgttttccgg cgactttgta gctgcggttg
    23161 gcggaggcgt gcagtttttc tgccttgacc actgcgtcgg cggatgccgt ctgaagggag
    23221 tgtgtgccga atgcggcggt gagaaggaaa agggtttttc gggttaaagt caaaacgtgt
    23281 tccgttcttg agttgaagac gaatgggcat catgcccgcc ggatacgttc cgaaccgccg
    23341 tacagtgcgg acggcggttc ggaatgtgtc cggataggtt ttcagacggc atgaacttgc
    23401 gttcaaacgc cgcctgcgta accgtgttgc cgccacgctt caaagagaat cacggcaacg
    23461 gtattggaaa ggttcatact ccggctgccg ggctgcatgg gcaggcggat tttttgcgcg
    23521 gcgggcaggc tgtcgaggat gtcggcaggc agtccgcgcg tttccggccc gaacagtaaa
    23581 acgtcgcctt tttgaaacgc ggtttcatcg gggcgcgccg tgcctttggt ggtcagggcg
    23641 aaaatgcgcc tgcctgcgag tgccttgagg cagtcgtcga agttttcgta caccgtcagg
    23701 ctggcgaatt cgtggtagtc gagcccggcg cgtttcattt tggcggaatc caatgggaag
    23761 ccgagcggtt tgacaaggtg caaatccgcg ccggtattgg cgcacaggcg gatgatgttg
    23821 cccgtgttcg gcgggatttc cggctggtat aaaacgatgg taaacataaa tatcaatcac
    23881 ttataggcgc gtaaccttgc cacaaggcgg atggggtgtc aaaaaattta gttatttttt
    23941 cattggcgtg cgtgccagcg tccagcagca gatgcggttc gcgcccgatt ttttcagcgt
    24001 ctttgccaat tcgtccagcg tcgcgccggt ggtaaagaca tcgtcgatta acagaatatt
    24061 acagttttcc ggtatcggtg tgcggatttc aaaggcgttt ttgatgtttc gccgccgttc
    24121 gctgcctttg agcgtgcttt gcggcgggcg gtggtgtcgg aatacggtgt gtcggggcag
    24181 tatctgccag ccgtagcgtt gtgccaacaa cccgacgatg ctttcacttt ggttgaaccc
    24241 gcgttgcagc agccgctccc tgcttagcgg tacgggcagg acgaaatcga aacattcgtc
    24301 tgcaagccgg tcgggcggat tctgcatcat caggtctgcc agcggctgca ccatgctcaa
    24361 atcagccaag tgcttcagcg cgtgtatcat attgctgacg ggcggttcgt aatgcagcga
    24421 agcccacatc cggtcgaatg cgggcggttt tttctgacag ccgccgcaca ccgcgccgcc
    24481 ttggatgtgc ctgaaacaca gggggcagct gtttgccgcg tcggtgcggt atgccgccaa
    24541 atcgtcgcgg cagccggcgc agatgccgtc tgaaacgccg gacgaaccgt ggcataatac
    24601 gcaacgcctg atagtgggcg cgtctgcgat gcgccgccaa cgagagagaa aatccatgcc
    24661 tgatgccgtc aaaaaagttt acctgataca cggttggggg gcgaaccgcc acatgttcga
    24721 cgatttgatg ccgcgcctgc ctgcaacgtg gccggtgtcc gccgtcgatt tgcccggaca
    24781 cggcgacgcg cccttcgcgc aacccttcga tattgaggca gccgccgacg ctgttgccgc
    24841 acaaatcgat acgcccgccg acattctcgg ctggtcgctc ggcggattgg tcgcgctgta
    24901 tctggcggcg cgccatcccg acaaagtccg ttcgctctgc ctgacggcga gtttcgcacg
    24961 gctgacggct gccgaagatt atcccgaagg gcttgccgcg cctgcactgg gcaaaatggt
    25021 cggcgcgttc cgttcggatt atgccaaaca tatcaaacag tttctacaac tccagcttct
    25081 gcacacgcct gatgcggacg gaatcatagg cagaatcctg cccgatttgg cacggtgcgg
    25141 cacgccctcc gccttacagg aagctttgga cgcggcggaa agggcggatg cgcggcattt
    25201 gttggacaag atagatgttc cggtactgct ggtgttcggc ggcaaagacg cgattacgcc
    25261 gccgcgtatg ggcgagtatc tgcaccggca tttgaaaggc agcaggctgg ttgtgatgga
    25321 aaaggcggca cacgcgccgt ttttgagcca tgcggaagcg tttgccgcgc tgtaccgcga
    25381 ctttgttgaa ggggtttgac atgaatccgc aggacaaatg ctggcaggtt caccgccatc
    25441 tcgccgaata tgccgaccaa cgcctgacac tcgtccgcaa cgcgcccaag tatatcctgc
    25501 ttgccggtgc ggatgcggac atcagccgca gcctgctggc gaaacgctat ccgcaggcgg
    25561 tatttgaaga atacgattcc cgtgcggact ttttggcggc tgccgctgcc gcccgcaaag
    25621 gcggtttttg gcaaaggttt acgggtaagg gcgtggtgca acactgccaa tccccgatcg
    25681 cgccgctgcc cgaagcgtgt gctgatatgt tgtggtcgaa tctcggactg ttggcggcgg
    25741 aacaaatcct tcctgtgctg cacaactggg cgtgcgcctt gaagacggac gggctgctgt
    25801 tttttacctg cttcgggcgc gacactttta ccgaactgaa atgccgtctg aaagaaaacg
    25861 gcattgaaag ccgcagcgtg atgtttcccg atatgcacga cttgggcgat atgcttgctg
    25921 aaaacggctt ttacgacccc gttaccgata cggcgaagct ggtgttggat tacaaaaagg
    25981 cggaaacgtt ttgggcggat atggacacgc tgggcgtttg gcgggcgatg gcgtggaacg
    26041 atgaaaacgc cgcgcgttcg tgtgtcggga caatatttga gcgggaaggc ggtttgggca
    26101 ttacgctgga aacggtgtac ggacacgccg tgaaaaaact gatgctgccg caaggggaga
    26161 acgtggtgca gttttttccg aagagatgat gtgcagatgc cgtctgaagc cgtttccagg
    26221 tttcagacgg catttgtctg tgaaaaccga cagaaataaa ggaaatgccg atgtatagtg
//
LOCUS       DAWFAD010000010        25999 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWFAD010000010 DAWFAD010000000
VERSION     DAWFAD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25999
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.010"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..761
                     /gene="mafB"
                     /locus_tag="WGO49_000305"
     CDS             <1..761
                     /gene="mafB"
                     /locus_tag="WGO49_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0305467.1"
                     /translation="GIGDILYGTRYAIDKAAMRNIAPLPAEGKFAVIGGLGSVAGFEK
                     NTREAVDRWIQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDFSAAYNTRT
                     TRKVTTETEGLNRIRQNQKNSNIHEKNYGRDNPNHINVLSGNSIQHILYGDEAGGGHL
                     FPGKPGKTTFPQHWSASKITHEISDIVTSPKTQWYAQTGTGGKYIAKGRPARWVSYET
                     RDGIRIRTVYEPATGKVVTAFPDRTSNPKYNPVK"
     gene            776..1006
                     /locus_tag="WGO49_000306"
     CDS             776..1006
                     /locus_tag="WGO49_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0305468.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            1155..1832
                     /locus_tag="WGO49_000307"
     CDS             1155..1832
                     /locus_tag="WGO49_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hint domain-/BECR-fold containing toxin"
                     /protein_id="HEZ0305469.1"
                     /translation="MVKTADGYKAIAHIQAGDRVLSKDEASGETGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLYSESGKTQTVRNTVV
                     KPKPLKAYNLTVADWHTYFVKGNRAETEGVWVHNECPPRKTPSTPIYGNDSEAYAAAK
                     ELGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPEKLNRKETRNGTFDK
                     NLNRIGD"
     gene            1838..2245
                     /locus_tag="WGO49_000308"
     CDS             1838..2245
                     /locus_tag="WGO49_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0305470.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRDIVIGIGTIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVEDWKFFYFPIQNSKGRLRRKV"
     gene            2267..2644
                     /locus_tag="WGO49_000309"
     CDS             2267..2644
                     /locus_tag="WGO49_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0305471.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRYVVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            2680..3165
                     /locus_tag="WGO49_000310"
     CDS             2680..3165
                     /locus_tag="WGO49_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pretoxin"
                     /protein_id="HEZ0305472.1"
                     /translation="MRLSDGIGNSQTLISNRIHSFYSGGKWIKTEDLKAGIRLLSESG
                     KTQTVRNIVVKPKPLKAYNLTVADWHTYFVKGNRAETEGVWVHNACPPKRTGSSKNEK
                     HGDDGRSQISAESRIAELKNKIIPGMPKNERLKIEQKIKNITKNANRKAKGEEHGRRG
                     R"
     gene            3146..3601
                     /locus_tag="WGO49_000311"
     CDS             3146..3601
                     /locus_tag="WGO49_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305473.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILETFMYEPPDEF
                     GTEYYESPDSNLLISVEKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            3608..3664
                     /locus_tag="WGO49_000312"
                     /pseudo
     CDS             3608..3664
                     /locus_tag="WGO49_000312"
                     /inference="COORDINATES: protein motif:HMM:NF026864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3670..4047
                     /locus_tag="WGO49_000313"
     CDS             3670..4047
                     /locus_tag="WGO49_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0305474.1"
                     /translation="MCEFKDFRRNIPCFEEYDENSFIGKWYDDGVWDDEEYWKLENAL
                     IEVRRKYPYPMDIPRDIVIGIGTIIEFLMVPNWKLFTIKSSPWLPKSVKIDERYERFR
                     VMLRYIFTEIDIVNVRFDYYNKK"
     gene            4131..4625
                     /locus_tag="WGO49_000314"
     CDS             4131..4625
                     /locus_tag="WGO49_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305475.1"
                     /translation="MENRFKKIVKDILSFAQFVSEAENKIPNQSSRYYDCWWELEILN
                     ASALEDWEISGKPEKWEKWTEKYQTEALQSVKHLFAEEVPENNIVCDISHFLHFADWA
                     EADIPNQSRLYAEYWWEMEMFKMSVLEDWERSGKPLDWKKWQRQYRSEAFELVKKLNR
                     SKQE"
     gene            4745..5878
                     /locus_tag="WGO49_000315"
     CDS             4745..5878
                     /locus_tag="WGO49_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305476.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEVPE
                     NPNAFVAKLARLFRNADRAVVIVKESMRTEESLAETVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLLQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHRENYETTGEYRVVWQPDGSVFDAAGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKQGDRLPDFPLNLENLEKEVRRYAEAAARRSGGRRDLSH"
     gene            complement(5965..6240)
                     /locus_tag="WGO49_000316"
     CDS             complement(5965..6240)
                     /locus_tag="WGO49_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305477.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLMCISVFWIVVFNL"
     gene            complement(6257..7024)
                     /locus_tag="WGO49_000317"
     CDS             complement(6257..7024)
                     /locus_tag="WGO49_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0305478.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNLEPMTGFE
                     HTVTFDFQGIKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPE
                     YEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNYHRYGENDYSTSVGSCINGFTVR
                     YYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(7436..7711)
                     /locus_tag="WGO49_000318"
     CDS             complement(7436..7711)
                     /locus_tag="WGO49_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0305479.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(7708..8991)
                     /locus_tag="WGO49_000319"
     CDS             complement(7708..8991)
                     /locus_tag="WGO49_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0305480.1"
                     /translation="MTVSKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(9145..10563)
                     /gene="glnA"
                     /locus_tag="WGO49_000320"
     CDS             complement(9145..10563)
                     /gene="glnA"
                     /locus_tag="WGO49_000320"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ0305481.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDVTVVITCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEGLGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(10837..11214)
                     /locus_tag="WGO49_000321"
     CDS             complement(10837..11214)
                     /locus_tag="WGO49_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305482.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVLVDSNTATFGDADSRTDNSA
                     WFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPSYAE
                     WNYVIPGTLGEAQYKFVCNGYPR"
     gene            11385..12194
                     /gene="aroE"
                     /locus_tag="WGO49_000322"
     CDS             11385..12194
                     /gene="aroE"
                     /locus_tag="WGO49_000322"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ0305483.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIPLKNGKLRGD
                     NTDGIGLTNDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            12196..12897
                     /gene="mtgA"
                     /locus_tag="WGO49_000323"
     CDS             12196..12897
                     /gene="mtgA"
                     /locus_tag="WGO49_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ0305484.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDAHFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(13100..13175)
                     /locus_tag="WGO49_000324"
     tRNA            complement(13100..13175)
                     /locus_tag="WGO49_000324"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13140..13142),aa:Phe,seq:gaa)
     gene            complement(13366..14100)
                     /gene="lptB"
                     /locus_tag="WGO49_000325"
     CDS             complement(13366..14100)
                     /gene="lptB"
                     /locus_tag="WGO49_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ0305485.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(14174..14704)
                     /gene="lptA"
                     /locus_tag="WGO49_000326"
     CDS             complement(14174..14704)
                     /gene="lptA"
                     /locus_tag="WGO49_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ0305486.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(14685..15266)
                     /gene="lptC"
                     /locus_tag="WGO49_000327"
     CDS             complement(14685..15266)
                     /gene="lptC"
                     /locus_tag="WGO49_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ0305487.1"
                     /translation="MKIRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(15263..15799)
                     /locus_tag="WGO49_000328"
     CDS             complement(15263..15799)
                     /locus_tag="WGO49_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0305488.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPMMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(15864..16838)
                     /locus_tag="WGO49_000329"
     CDS             complement(15864..16838)
                     /locus_tag="WGO49_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ0305489.1"
                     /translation="MAENGKYLDWAREVLHTEEEGLREIATELDENFVLAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            16935..17990
                     /gene="tal"
                     /locus_tag="WGO49_000330"
     CDS             16935..17990
                     /gene="tal"
                     /locus_tag="WGO49_000330"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ0305490.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVQAACDVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRVQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWAQYFGSPEFAALETQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGADEAQAQLAETAALGIDVETLAARLQED
                     GLKQFEEAFEKLLAPLV"
     gene            18144..18434
                     /locus_tag="WGO49_000331"
     CDS             18144..18434
                     /locus_tag="WGO49_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305491.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            18451..19338
                     /gene="gluQRS"
                     /locus_tag="WGO49_000332"
     CDS             18451..19338
                     /gene="gluQRS"
                     /locus_tag="WGO49_000332"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ0305492.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAAASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLQTAGLVYPCHCSRK
                     DWQAAAVHGADGFVYNGRCRNPQQRPALQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLKLPEAPETDRPAELLD
                     WAVAHWDMGKVPKHAITTP"
     gene            complement(19408..20418)
                     /gene="dusA"
                     /locus_tag="WGO49_000333"
     CDS             complement(19408..20418)
                     /gene="dusA"
                     /locus_tag="WGO49_000333"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ0305493.1"
                     /translation="MNDNTHTLPPRHLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQCLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(20449..20940)
                     /locus_tag="WGO49_000334"
     CDS             complement(20449..20940)
                     /locus_tag="WGO49_000334"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ0305494.1"
                     /translation="MRAVIQKTVGAKVDVVSEAGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(21014..21772)
                     /locus_tag="WGO49_000335"
     CDS             complement(21014..21772)
                     /locus_tag="WGO49_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0305495.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRNPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDALLEENGVKP"
     gene            complement(21843..22709)
                     /locus_tag="WGO49_000336"
     CDS             complement(21843..22709)
                     /locus_tag="WGO49_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0305496.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(22758..23036)
                     /locus_tag="WGO49_000337"
     CDS             complement(22758..23036)
                     /locus_tag="WGO49_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0305497.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(23036..23326)
                     /locus_tag="WGO49_000338"
     CDS             complement(23036..23326)
                     /locus_tag="WGO49_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ0305498.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(23329..23859)
                     /locus_tag="WGO49_000339"
     CDS             complement(23329..23859)
                     /locus_tag="WGO49_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ0305499.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            24167..>25999
                     /locus_tag="WGO49_000340"
     CDS             24167..>25999
                     /locus_tag="WGO49_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ0305500.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPS"
BASE COUNT         6801 a         6697 c         6312 g         6189 t
ORIGIN      
        1 tgggcatagg cgacatactg tacggaacgc gctatgccat agacaaagcc gcaatgcgca
       61 acatcgcccc cttgcccgcc gagggcaaat tcgccgtcat cggcggcttg ggcagcgtgg
      121 cgggctttga aaaaaatacg cgtgaagccg ttgaccggtg gatacaggaa aaccccaatg
      181 ccgccgaaac cgtcgaagcc ctggtcaacg tcctgccgtt tgccaaagtc aaaaacctga
      241 caaaggcggc aaaaccgggg aaggctgcgg ttagcgggga tttttctgct gcatacaata
      301 caagaacaac tagaaaagtt actacagaaa cagaggggtt aaatagaatc agacagaacc
      361 agaaaaatag taatatacat gagaaaaatt atggaagaga taatcctaat catattaatg
      421 ttttatctgg aaattctata caacatatac tgtatggaga tgaagcagga ggtgggcatc
      481 tttttcctgg caaacctggt aagacaacat tcccccaaca ttggtcagcc agtaaaataa
      541 ctcatgaaat tagtgatatc gttacatccc caaaaacgca atggtatgca cagactggaa
      601 caggcggcaa atatattgct aaaggaagac cagccaggtg ggtatcatat gaaacgagag
      661 atggaattcg tatcagaaca gtttatgaac ctgcaacagg aaaagtggta actgcattcc
      721 ccgatagaac ctctaatccc aaatataacc ctgtaaaata atgagtattt gaaagatgaa
      781 aacattagac gaacgaataa aaaatttagg aaaatctctt gaagatagaa ttgacgctaa
      841 tctaattgat gccgcactag agtacattac tttttcagaa cgtttgctag cttttgaaac
      901 gctatgtgat tatatagaag atttcaatat ccagcttacg gaaaaagaat cccaagaaat
      961 ctcttttatt aataaggaat ttggaattga aagtacatca gattaataaa tattaggtcg
     1021 gtttaaaaac ccaacaatca aaaagtcgtc tgaaactgtg tttaatttac agacgacctt
     1081 tccttcattt gaagcaggat attgagaact gagttcttca aaaatcctac acctgctcct
     1141 tccacggcag caccttggtc aaaacggcag acggctacaa agccattgcc catattcaag
     1201 ccggagaccg cgtcctttcc aaggacgagg caagcggaga aacgggatac aaacccgtta
     1261 ccgcccgata cggcaatccc tatcaagaaa ccgtttacat tgaaatttca gacggcatcg
     1321 gcaacagtca aaccctgatt tccaaccgca tccatccgtt ttattcggac ggcaaatgga
     1381 ttaaggcgga agatttaaaa gcgggaagca ggctgtattc cgaaagcggc aaaacccaaa
     1441 cagtccgcaa caccgttgtc aaaccaaaac cgctcaaagc ctacaatctg accgttgccg
     1501 attggcatac ttactttgtc aagggcaatc gggcggaaac ggaaggggtt tgggttcata
     1561 atgagtgtcc gccaagaaaa acaccatcta cccccatata tgggaatgat tcagaagcat
     1621 atgcagcagc aaaggaattg ggatatagaa aaataaaaga aagaacgaga aatgatgctg
     1681 ctatttttaa aaaaggaaag agttatatat cacgcgatgt agattcacat aatggaggtg
     1741 cttggaaaga ggcatcttca cccgaaaaac tgaacagaaa agaaacaaga aacggtacat
     1801 ttgataaaaa cctaaatcgt attggagatt aattattatg tgcgagttca aggattttag
     1861 aagaaacatc ccttgtttta aggagtatga cgaaaattca tttattggca aatggtatga
     1921 tgacggggtg tgggatgatg aagaatattg gaaattggag aatgatttaa ttgaggttag
     1981 aaaaaaatat ccttatccga tggatatacc aagggacatc gtgattggaa tcggtaccat
     2041 tattgatttt ttaatggttc aaaattggaa actttttgaa attaaagctt ctccttggtt
     2101 gcctaaaagt gtcaaaatta atgagcgtta tgaaaggttc agggtaatgc tccgttatat
     2161 ttttactgat ctagatgtag aggactggaa atttttttac tttccaatac aaaatagtaa
     2221 aggtagattg agacgaaaag tttaattata ttggagatac attattatgt gtgagttcaa
     2281 ggatattata agaaacgttc cttattttga ggggtatgac gaaaattcat ttattggcaa
     2341 atggtatgat gacggggtgt gggatgatga agaatattgg aagttggaga atgatttaat
     2401 cgaggttaga aaaaaatatc cttatccgat ggacatacca agatatgttg tcattggaat
     2461 cggtaccatt attgatttct taatggttcc aaattggaaa ctttttgaaa ttaaagcttc
     2521 cccttggcta cctgatagcg tgggaattaa cgaacgttat gaaaggctta aaacaatgct
     2581 ccgttatatt tttaccgaga aagacatagt caacgtgcga tttgattatt acaacaaaaa
     2641 atagcaaaac ccggcaatca aaatgccgtc tgaagcgata tccggctttc agacggcatc
     2701 ggcaacagcc aaaccctgat ttccaaccgc atccactcgt tttattcggg cggcaaatgg
     2761 attaagacgg aagatttgaa agcgggaatc aggctgttat ccgaaagcgg caaaacccaa
     2821 accgtccgca acatcgttgt caaaccaaaa ccgctcaaag cctacaatct gaccgttgcc
     2881 gattggcata cctactttgt caaaggcaat cgggcggaaa cggaaggagt ttgggttcat
     2941 aatgcgtgtc cgcccaaaag aacaggaagc tccaagaatg aaaaacatgg agacgacggt
     3001 cgaagtcaaa tatcagcgga atcaagaatt gctgaattaa aaaataaaat tattcccgga
     3061 atgcccaaaa atgaacgctt aaagattgag caaaaaatca aaaatattac aaaaaatgcc
     3121 aatcgaaaag caaaaggaga agagcatggt cgacgcggtc gttaaaactc ccgaattttt
     3181 accttttact tctgtaggaa tattccgttt tggtgctgat ataacacagt acaaaaacat
     3241 actggaaact tttatgtatg aacctcctga tgagtttgga acagaatatt atgaatctcc
     3301 cgactcaaat ttactcattt ccgtcgaaaa aaacaaaatt atttcgatat tttgttatca
     3361 ggagctatat tttatgggcg ttaatataat aggtctgaat tttgaagatt ttaaacagct
     3421 tttccatcac cctaaatcat acggtgttga taaatattat ttatcaaatg aatcttatcc
     3481 gacatatgtc tatgagtttg atgaaatagg agtacaagct tgggaagcaa aagggaaaat
     3541 tgttactatc attgccgggg gcaaagacaa ttattcgaca gaaccttact acgatgaata
     3601 acattacaga aaagaaacaa gaaacggtac atttgataaa aacctaaatc gtattggaga
     3661 ttaattatta tgtgcgagtt caaggatttt agaagaaaca tcccttgttt tgaagagtat
     3721 gacgaaaatt catttattgg caaatggtat gatgacgggg tgtgggatga tgaagaatat
     3781 tggaaattgg agaatgcttt aatcgaggtt aggagaaaat atccttatcc gatggatata
     3841 ccaagggata tcgtgattgg aatcggtacc attattgagt ttttaatggt tccaaattgg
     3901 aaacttttta caattaaatc ttccccttgg ttgcctaaaa gtgtcaaaat tgatgagcgt
     3961 tatgaaaggt tcagagtaat gctccgttat atttttaccg agatagacat agtcaacgtg
     4021 cgatttgatt attacaacaa aaaatagcaa aacccggcaa tcaaaatgcc gtctgaaacc
     4081 gtgtttgatt tttgacgaca ttttcctcat tggaggggcg gcaattattt atggaaaata
     4141 gatttaaaaa aatcgttaag gatattctta gttttgccca atttgtttcc gaagcggaaa
     4201 ataaaattcc taaccaatcc tctcgttatt acgattgctg gtgggaattg gaaatcttga
     4261 atgcttccgc attggaagat tgggaaatat cgggaaagcc tgaaaaatgg gagaaatgga
     4321 cagaaaaata tcagaccgaa gcgttacagt cagtcaagca tctatttgcc gaagaagttc
     4381 ctgaaaataa tatagtttgc gatatttctc attttttgca ttttgccgat tgggcagaag
     4441 ccgacattcc caatcaatca cgtttatatg ccgaatattg gtgggaaatg gaaatgttca
     4501 aaatgtcggt tttagaagat tgggaacgct cgggaaaacc gttagattgg aaaaaatggc
     4561 aaaggcaata tcgatctgaa gcatttgaat tagttaaaaa attgaaccga agcaaacaag
     4621 agtaacaagc cgcagcctgc ataagggttt ttaaagtacg gcagcaaccg tagattaaat
     4681 taaaaatcaa acaatcaaag ctcgtctgat ataatcgata tattctgtta aacgaaaaat
     4741 aaatatgttt aaaaaattca aaccggtact gttgtcattt tttgcacttg tatttgcctt
     4801 ttggctggga acgggtattg cctatgagat taatccgcgt tggtttttga gcgatacggc
     4861 aactgaagta cctgaaaatc cgaatgcttt tgtggcgaaa cttgcccgcc tgttccgaaa
     4921 tgccgacagg gcggttgtca tcgtgaagga atcgatgagg acggaggaaa gtcttgccga
     4981 aactgtggat gacggtccgt tgcagtcgga gaaggattat ctcgcgctcg ctatccggct
     5041 cagtcgtttg aaagaaaagg cgaaatggtt tcacgtaacg gagcaggaac atggggaaga
     5101 ggtttggctg gattactata tcggcgaggg cggtttggtt gcggtttcgc ttttgcaacg
     5161 ctcgccggaa gcgtttgtta atgccgaata tctgtatcgg aacgatcgtc cgttttctgt
     5221 aaatgtgtac ggcggaacgg ctcacaggga aaattatgaa acgacaggag aatatcgggt
     5281 tgtttggcaa ccggacggtt cggtatttga tgcggcaggg cgcgggaaaa tcggggaaga
     5341 tgtttatgag cattgcctcg ggtgttatca gatggcccag gtatatttgg cgaaataccg
     5401 ggatgtcgcg aatgatgagc agaaggtttg ggacttccgc gaagagagca accggattgc
     5461 atcggactcg cgcgattatg tgttttatca gaatatgcgg gaattgatgc cccgggggat
     5521 gaaggcaaac agtcttgtgg tcggctatga tgcggatggt ctgccgcaga aagtctattg
     5581 gagtttcgac aatggaaaaa aacgccagag tttcgaatat tatttgaaaa acggaaatct
     5641 ttttattgca caatcttcga cggtagcatt gaaagcggat ggcgtaacgg cggatataca
     5701 gacctatcat gcgcaacaga cgtggtattt ggatggcggg cggattatcc gcgaagagaa
     5761 acagggagac agactgcctg attttccttt gaacttggaa aatttggaaa aagaggtgcg
     5821 ccgttatgca gaggctgcgg cgagacgttc gggcggcagg cgcgaccttt ctcactgaac
     5881 gcaaatgccg tctgaaaccg gttttcagac ggcattggag agtcaaacgc tttttgcaat
     5941 gccgtatatg ccgaatccga ttcgctacaa attgaaaaca acaatccaaa aaacggaaat
     6001 acacattaaa cgggtctttt taccgaccgt atcatcccat aggttttcca cacctttttt
     6061 catccataaa ttcaaaccat ttaagtttgc atccttaccg acaattgcag cagcatcatc
     6121 cgcataacgg tcacccaaat tagccaaata acgccaaccg ccataggtat cgccacggaa
     6181 agcataacca cgcaaggttt ggatttgttc tggtgttaaa acaacttcac cctcattcat
     6241 aataagatcc tttaaattat tttttacttg aattgtttac aaaactctgt accaattgct
     6301 ctacttgttg gtgataacct accaactcct gctgtgtgag ctgctgcttt tcccgaataa
     6361 acgggtaata ccgtaccgtg aaaccgttaa tacaggaacc tacgctagtg ctgtaatcgt
     6421 tctctccata cctatgatag ttccgatgag taataatcgc cggattttct ccttttttaa
     6481 tatagtaaaa ttcatgaaaa tatggatttt caggaatatg tcttttatct tcataagcct
     6541 cgtattcagg ataggcagat ggcatttttt taaccccgta agtaagcaat ctttctccca
     6601 tagtcgtgct taaactacac gcatcttttc ggataacaat atcgtccacg ctatccaaac
     6661 cgtcgcgtag aaattggata aagtgcgctt tgttttgctg gttgtatggc tcaagaaccc
     6721 agccttggtc ggtttttttg taatagacgc taatctctat caaattaatg gagtaagcat
     6781 cctgcccggg cgtgtcggaa agccattttg tggcattgtc ttgcgtatac cgtgcaagat
     6841 agccataagg gataaccatt ttgatgccct gaaaatcaaa tgtaaccgta tgttcaaatc
     6901 ctgtcattgg ctcgagattg ttgaaactgg tttgttcatt aacaggttca catgaggtca
     6961 tagtaaaaac actcccaata aacaaaataa aaagttgata aatggtagta gcatatggtc
     7021 tcataatttc aagcttagaa attagttaaa gaatatattc atggtagata ctaactcttt
     7081 aagttgacat attgtgcgat gtcgagggct gtaccgtatt ttcccatcgt attttcccat
     7141 cgtattttcc catcgtattt tcccgttctt tatatgagcg gcaggcatcc tcataaaaga
     7201 agggataccg ataaggaaaa ataccccagc ataaaaaata tactgattaa tcaatacaaa
     7261 tccaacaaac agttcatcgt attcttttct gttggcgtat aaaaatactg atatattcct
     7321 tcactttcat agggcgacgc tttatgtacc gtcctgtgtg ttgaaacgtc ggttttcctt
     7381 accgcctgcc ccgcttttcc ctgattgctg ccaactgccg tccgaacttg aaccgtcagg
     7441 tttcagacgg cattttttct gcctgctccc acaattgccg tttttgtgcg gcggtcagtt
     7501 ttttgacggc gatttcctgc ctgagtgccg tgcctttatc catcccgcct gcaacgatac
     7561 gcatcgccac cggtttgaat acgcgggtat atttcgcgcc tttaccggtt gtgtgggcgg
     7621 caagccgctg ttgcggattc gggctgatgc cgcaatagaa cgcgctgttt tcacacaata
     7681 tcaggtaaac gctccagttt gacgcgttca tctgcctgca tcctgagttt tctccccgtt
     7741 ccaaggcgca actttcagca gcagcagcat acccggtagg gcgagtgcga aacacagttg
     7801 gaaaaacggt acataaccga gccattcaat cagataaccg gcaaaggaat tgatgaccgt
     7861 gcgcgggacg gcggacaggc tggtaaacag cgcaagctgc gttgcggtaa atgcgggatt
     7921 ggtttcacgc gccatatacg atacgaacgc cgccgtcccc aaccccacgc cgaccgcttc
     7981 cgcgccgata actgccgcca gcatcagcct ctcgcctgtg ccgaccgtgt cgaaaggtcc
     8041 gaaccctgcc agccatacaa accccaaaac ggttacagcc tgcaccgcgc caaatagcca
     8101 caaggctttg tttacgccga ttttcagcat ccacacgccg cccaagatac ctgccgccac
     8161 tgccggccac agtcctgcat ttttcgcaat caaaccgatg tctgtcttgc tgaaacccat
     8221 atccagataa aacggcgttg ccaacgcggt tgccatactg tcgccgagtt tgtaaaggaa
     8281 gataaacagc agcacgcaca ccgccgaagc gatgcccttg cgcgtaaaaa attctttaaa
     8341 cggctctacc acggtctgct tcaacgtttt aggaacggca ggcggcaaca cgggttcgcg
     8401 cgcaagaaac agcgtcatca gaaggccggg cagcataaat aatgaagtga taacaaatac
     8461 ttctgaccac ggcatcctgt ctgccaacac caaactcaat gaaccgggaa tcagggcggc
     8521 aacccgatag gcgttcacat gaaccgagtt gcccaaaccc aattcttcgt ccgacaaaat
     8581 ctcgcgcctg aacgcatcca atacaatgtc ctgactggcg gaaaaaaaag cgacaagcac
     8641 cgacaagccg gcaatcagcg gcagatgatt acgggggttt aaaaaggcat aggccgccaa
     8701 agccgccagc aaccctgcct gcgtcagcag catccacccg cgccgccgtc ccaaaacggg
     8761 cagcctgacc gcgtccatca gcggcgacca caaaaatttc caagtaaacg gcagaccgat
     8821 taacgccatc agcccgatgc tcttcaaatc cacctgctcg ctgcgcaacc acgccggaat
     8881 caggttaatc agaaagtaca gcggcagccc cgaggtaaac cccgtaaaaa tacaaacaag
     8941 catatttcgg gaaaagattt gcccgataaa acctgatttc gatacagtca tggttcaaac
     9001 agccaagaaa taaaagccta ttgtagcaaa tatcgcccaa ccgctgaaaa acaccgcccc
     9061 tgctccgccg ttttgccgtt caaatgcaaa atgccgtctg aaactgtgtt cagacggcat
     9121 tgcttttctg aaaacccgac gtgcttacag gctgtaatac atttcaaatt ccagcggatg
     9181 cggtgccatg cggatgcggc ggacatcttc ctctttaaag gcgatatagc tgtcgatcca
     9241 gtctttgctg aacacgccgc cgcgcaggag gaattcgtgg tcggctttga gggcggcgag
     9301 ggcttcttcc aaagaggcgc aaacggtcgg gacgagcgcg tcttcttccg gcggcaggtc
     9361 gtacaggttt ttatcggcag gatcgcccgg atggattttg ttttgaatac cgtccaaacc
     9421 cgccatcaac agggcggcaa atgccaaata cgggttggcg gtcggatcgg ggaaacgcgc
     9481 ttcgatgcgg cgcgccttgc tgctgttcac ggacggaatg cggatggaag cggaacggtt
     9541 tttggcggaa tatgccagtt tggtcggtgc ttcaaagtgc ggcacgaggc gtttgtagga
     9601 gttggtggac ggattggtaa ttgcgttcag ggctttggcg tgtttgatga tgccgccgat
     9661 gtagtagagc gcggtgtcgc tcaagccggc atagccgtcg cctgcgaaca ggttttgacc
     9721 gtctttccaa atggattggt gaacgtgcat accgctgccg ttgtcgccca taatcggttt
     9781 gggcataaag gtggctgttt tgccgaagtt gtgggcaacg ttttgaatca catatttcat
     9841 gtcttgggtt tggtcggcgc gtttgaccaa ggtggcgaaa cgcgtgccga tttccatttg
     9901 gctgccggta ccgacttcac tgtggtggac ttcgacttcg atgccgagtc cttccaaaat
     9961 gtttaccatc gcggaacgca aatcctgacc gcagtcaatc ggcgcgacgg gcgcgtagcc
    10021 gcctttgacg gcgggacggt ggccggtgtt ttgaccgtcc atatgcaggc cgcttgccca
    10081 cgcgccgctt tcggacgtga tttcgtaacg ggttttgtgc atgtcggttt caaattctac
    10141 gccgtcgaag acgaagaatt cgggttcggg gccgaagtat gccgtgtcgc cgatgccgga
    10201 agatttcaaa taggcttcgg cgcggcgggc gatggagcgc gggtcgcggt cgtaaccctg
    10261 accgtcggca gggtcgatga cgtcgcaggt aatgacgacg gtaacatcat cataaaaagg
    10321 atcgacgaag gctgtagacg catcgggacg caactgcata tcggaagcct gaatgccttt
    10381 ccagccgccg atagacgaac cgtcaaacgc ctgaccgttt tcgaaccact cttcggggtc
    10441 ttccaacacg atgcgcgcag gcacggtaaa gtggtgctgc ttgcctttgg tatcggtaaa
    10501 gcgcaaatcg acaaagcggg cttcgctttc ttcaatcaat tttacggcgt ttttaataga
    10561 catttttcag ctcctggaaa agggaaatag caaaatatgc ggaacgcggc agattagctc
    10621 aaagccagca tgattctacc acgtccatgc cgtctgaaac tggccctgcg tcaaattcca
    10681 agctgtaaaa tatagtttca gacggcaata ttgtcaacaa tatgcttcgg tttgcccgtt
    10741 cccaagcaga atgcccgccc ttttatccga atgccgacaa aacagatgcc gtctgaaagc
    10801 agttcagacg gcatctgttg tttggtttga aaacggtcag cgaggatagc cgttgcagac
    10861 aaatttgtac tgagcctctc caagcgtacc gggaataaca taattccatt cggcatagga
    10921 tggtgtataa gaagcgatag agtttcccga tttggaatac cacgttgccg tcaatatcct
    10981 aaaacgttta ccactgcaat ccatttcttc caacgtttta gctgtattaa aaaattttcc
    11041 attgccatac ttttgcggct tggcgaacct tgtttcaaac catgcactgt tgtccgtacg
    11101 gctgtctgca tcaccaaacg tggcagtatt actatctacc aatacccaat ccgccgccac
    11161 cgccggtgtt gccaacccca atgcaaatac tgccaagcac aacatctttt tcatgtccga
    11221 ccctctcgtc cttgtcaaca aaataaagtt aaattgtaaa aaaaaacgac ttttttcaag
    11281 agaaaaatat tccgtcatgc tatctgacaa ccgtttcaga cggcatcgtt tccatctgta
    11341 cgccgccgtt ataaaatagc cttcccgtct tttggaacac gaccatgacc gccctccccc
    11401 gttactccgt ttttggcaac cccgttgcac acagtaaatc gccgcaaatc catcagcaat
    11461 ttgcccttca ggaaggcgtt gacattgaat acgaacgcat ttgcgccgac atcggcggtt
    11521 ttgcacaggc ggtttcgaca ttttttgaaa caggcggttg cggggcaaac gttaccgtac
    11581 cgttcaaaca ggaagcgttt catctggcgg acgagcattc cgaacgcgca ttggcggcag
    11641 gtgcggtcaa tacgctgatt ccgttgaaaa acggcaagct gcgtggcgac aacaccgacg
    11701 gtatcggttt gaccaacgac atcacgcagg tcaaaaatat tgccatcgag ggcaaaacca
    11761 ttttgctttt gggcgcaggc ggcgcggtgc gcggcgtgat tccggttttg aaagaacacc
    11821 gccctgcccg tatcgtcatt gccaaccgta cccgcgccaa agccgaggaa ttggcgcagc
    11881 ttttcggcat tgaagccgtc ccgatggcgg atgtgaacgg cggttttgat atcatcatca
    11941 acggcacgtc cggcggtcta aacggtcaga ttcccgatat tccgcccgat atttttcaaa
    12001 actgcgcgct tgcctacgat atggtgtacg gctgcgcggc aaaaccgttt ttagattttg
    12061 cacgacaatc gggtgcgaaa aaaactgccg acggactggg tatgctagtc ggtcaagcgg
    12121 cggcttccta cgccctctgg cgcggattta cgcccgatat ccgccccgtt atcgaataca
    12181 tgaaagccat gtaatatgtt ccgcatcgtc aaatggctga ttgccctgcc cgtcggcatc
    12241 tttatctttt tcaatgccta tgtgtacggc aacatcatta cctaccgcgc cgtcgcgccc
    12301 catcggactg cctttatgtc gatgcggatg aagcagtttg aacaggaagg tcgcgatgtc
    12361 gcactggatt accgctgggt gccctacaac cgcatctcgg tcaacctgaa aaaagccctg
    12421 attgcttccg aagatgccca ttttgccggg cacggcggct tcgattgggg cggcattcaa
    12481 aacgccatca ggcgcaaccg gaacagcggc aaagtgaagg cgggcggctc gaccatcagc
    12541 cagcagcttg ccaaaaacct gtttttaaac gaaagccgca gctatatccg caaaggcgaa
    12601 gaagcggcga ttaccgcgat gatggaagcc gttaccgaca aagacaggat ttttgaactg
    12661 tatttaaact caatcgaatg gcactacggc gttttcggcg cggaagccgc gtcccggtat
    12721 ttttatcaaa tacccgccgc caagctgacc aaacagcagg cggcgaaact gactgcccgc
    12781 gtccccgccc cgctctacta cgccgaccat ccgaaaagca aacggctccg caacaaaacc
    12841 aatatcgtgc tcagacgcat gggttcggca gagttgcctg aaagcgacac ggactgagtg
    12901 ccgccgcaat ataaaatgcc gtctgaaata tcattcagac ggcatatgct ttctgccttt
    12961 acccttttct ctaaaacgca acggggcggt cattatcgct tttgtccttc tacataacgg
    13021 tcagacacaa aacgtgtccg acacggacaa cttcccaaaa acggcaggca aaaaaaataa
    13081 ccgcttataa gcggtttttt ggtggccaga ggcggaatcg aaccgccgac acacggattt
    13141 tcaatccgtt gctctaccaa ctgagctatc tggcctttgt ctgtctcgtt aagagatgcg
    13201 tattaaacca aattacggga cgacatgcaa gcatatcggg caatattctt tgaaaacagg
    13261 tttaagtcaa tgttttaaat ttgaattaat ttatgtaaaa acaacataaa tccgtatttt
    13321 tgccgcctgc ccgacgatat taggtcgccc gtctgaaaaa tattttcaat atttgaagtt
    13381 cttacccaga taaacagaac gaacctgttc gtttccgacc aaatcatcag gttttcccga
    13441 tgccaacacc gtgccgtctg aaataatgta ggcccgatcg cagatgctga gggtttcgcg
    13501 tacgttgtgg tcggtaatta atacgccgat accgcgcgac ttgagaaatc cgatgatttt
    13561 ttggatgtcg atgacggcga tgggatcgac gccggcaaaa ggctcgtcca acaaaataaa
    13621 atgcggtttc atggcaagta cgcgggcgat ttcgacgcgt cggcgttcgc cgcccgacaa
    13681 agatggggca ggattattgc gcaaacgctc gatattcagg tcggcaagca gtttttcaac
    13741 ttcgccgtcg atgcggcttt tgtctttggt actgatttct aaaatggcgc ggatgttttg
    13801 ttcgacggtc attttacgga aaatagaggc ttcctgcggt aggtagccga cacccaggcg
    13861 ggcgcgttcg tgtatgggca ggtgacgcaa ttcctgcccg tccagcatca cgctgcccgc
    13921 atcggcggca atgagtccaa caatcatgta gaagctggtg gttttacccg caccgttggg
    13981 cccgagcagg ccgacgactt ccccgctttc gatttcaagg gagaagcttt taacgacttg
    14041 gcgttttttg aaactttttt gcaggttttg aacaacaagg cggctgacgt ttgcgctcat
    14101 aaatatctct tcaatatctt cggtcggcaa atgccgtccg aaccaggttt atgtttcaga
    14161 cggcattttg ggattattcg gatttttgcg tactcgaagg ctggataacg acgctgaccc
    14221 tgccggattt ggaagcggat tttgcgccgg attttgtgct gccgctgatg gtatagactt
    14281 cggttttggt gttgtatgta atcaccgcac cttcggcgac atcgccgccg cgctgtactt
    14341 tggcattacc ggttaagact acggtgctgc ctgcagatga ataagcaacg ttgtttgcct
    14401 gtccgcgcac cgtgcctttg ccgccgtcca atgtctggct gaagcggact ggcgaacctt
    14461 ccgccctcac ggattcgccg cctttgccgc cgcgtgtaac attgacgcgg gcggcggaaa
    14521 tattgagcgt accctgtctg atgacgacgt ttccgctgaa tgtggtgctt tggttggctt
    14581 gatcgagcga accttggtcg gcctcaatct gaataggctg cctgctgtcg ctttgaaggg
    14641 caaaagcggg ggacgccgaa aaaaatgcta ttaaaacaaa tagcttacat atcttttgta
    14701 tcataaatcg tggctttcac tttagatgag aagttcaaca tgcctgtttt gtggtcgtaa
    14761 gtcatgccgc ccgcctgacc gtgcgatgca ccatattgga aactgacagg cgtatcggtt
    14821 tgggcatatt gagattcggt atcgacgtgc agcttttcgg cttcaacttt acccgcctgc
    14881 cgtttgccgt cagcggtttt ggtcagcaca acgttgtttt taaaaagaac ctgtttgttt
    14941 tcggtatggt aaacggcttc atcgctgccg acttcgtaca acaacctgcc ttcttggaag
    15001 aacacgagat gcggcgaatc aaaatggatg tcgctgcttt cgggaaactg tttcgcgcct
    15061 ttcgcgctca aatgttcttt taagtatccc tgttcgtcaa accgcctgcc atccaagccg
    15121 tccattgtgt attgcggttc gtcgggattg agcctgactt cttcaatctc gacttcgctg
    15181 atgcgtccca accatgccga cagaccgccc aaggcaaccg ccaatatcaa tgggaacgca
    15241 attccgtacc gccatcttat tttcatttga tgtactcgtt caaagccgcg cccaaagtcc
    15301 cttgcgcctg cataatcagg tcgcacactt cgcgcaccgc gcccgcaccg ccggggcgtt
    15361 tggcaatata ggcggcgtgt tgcagcgtaa accaatgcgc gtcgggaacg gcaaccggca
    15421 atccgcaacg caccatcatc ggcaaatcga ccacgtcgtc gccgacaaag gcacactcgg
    15481 cttcttccac gccgacctga gcgcgcagct cttcatacgc ggcgcgtttg tccgaaatgc
    15541 ctttgaagta gtaatttatg cccaactgtt tgacgcggat gccgacggag ggcgcgtccc
    15601 gacctgtgat aatcgcagtc tgcacgccgc ttgcctgaag cattttcaga ccgtgtccgt
    15661 ccagtgtgtg aaacgatttg atttcttcgc cgttatcgcg gataaagatg cgcccgtcgg
    15721 tcaaaacgcc gtccacatcc aggatcaaca gtttgatttt ggcggcgcgc gcctgtaatt
    15781 cgggagaaat tgcctgcatg ggatttcctt gttttcagac ggcatatctt accacaaatg
    15841 ccgtctgaaa cggctgcaac gccttataca atccgtgccg ccagcaggtc gtgcatattc
    15901 agcgcgccga tcagcacgcc atctgcatcg gtaaccagaa gcccgttcac atggtttgcc
    15961 tgcatgactt tcagggcttc ggtggcgaga cgttcggcgg agatggtttt aggatgcgta
    16021 tgcatgactt cgtctatcga aagaccggta aaattgtcgc attcttgaaa caggcggcgc
    16081 aaatcgccgt cggtgaatac gcctttcaga cggccttgcc cgtccgttac cgccaacatg
    16141 cccagccctt tttcactcat gctgacgatg gcttctttca agggcgtgcc gagtcggacg
    16201 gcaggcaggc cgccgccttt gtgcataatg tcggcaacgc gcaaaagtag gcgtttgccg
    16261 aggctgccgg caggatggct caaggcgaaa tcgtcgggcg tgaacgcgcg tgcgcgcagc
    16321 aggacgaccg ccaacgcatc gcccaaagcc atgacggcgg tggtgctggt ggtcggggca
    16381 agccccagcg ggcaggcttc tttggaaacc gacgccgtga tgtggatgtc ggcatggcgc
    16441 gccatggttg aatcggggcg ggcggtgatg cagacaagcg tgatgtcttt gcgtttgagc
    16501 gcggggatga tggcggcgat ttcgtcgctt tcgccggaat tggaaatcgc gacgaccacg
    16561 tcgttgtcca caatcatgcc caaatcgccg tgtgccgctt ccgcagggtg gacaaaaaac
    16621 gcgggcgtgc cggtcgaggc catcgttgcc gccattttgc gcccgatatg tcccgacttg
    16681 cccatgcccg tgataacgac cctgcccttg cagtgcaaca acgcgtctgc cgcaaggacg
    16741 aagttttcgt ccaattccgt tgcaatttcg cgcaagcctt cctcttcggt gtgcaacact
    16801 tcgcgcgccc agtcgagata ttttccgttt tctgccattg ccatccgcct ttgctgtaat
    16861 aaaactaccc aaaatttccg aatcggggta gcataagcct ttgaaacata cacatccatc
    16921 caaaggaatc agaaatgact attttatcgg acgttaaagc attaggacaa caaatctggc
    16981 tggacaacct ttcccgctcg ctcgtgcaaa gcggcgaatt ggcgcaaatg ttgaaacaag
    17041 gcgtgtgcgg cgtaacttcc aatcccgcca ttttccaaaa agccttcgcc ggcgatgccc
    17101 tgtacgccga tgaagtcgcc gcgctcaaac agcaagatct gaccccgaaa caacgctacg
    17161 aaaccatggc ggttgccgat gtgcaagccg cctgtgacgt gtgccttgcc gaacacgaat
    17221 ccaccggcgg caaaaccggt ttcgtcagcc tcgaagtttc gcccgaattg tccaaagacg
    17281 cgcaaggcac ggtagaagaa gcgcgccgcc tctacgccgc catcgggtgc aaaaacgcga
    17341 tgattaaagt gcctgccacc gacgcaggca tcgatgcgct cgaaaccctc gtttcagacg
    17401 gcatcagcgt aaacctgacc ctgctgttct cacgcgtcca aaccctcaaa gcctacgccg
    17461 cctacgcgcg cggcattgcc aaacgcttgg cagccggaca aagcgttgcc catatccacg
    17521 tcgtcgccag cttcttcatc tcccgtgtgg acggcgcgct ggacacaaca ctgcccgacc
    17581 acctcaaagg caaaatcgcc atcgcccttg ccaaagccgc ctaccaagac tgggcacaat
    17641 acttcggcag cccggaattt gccgcactgg aaacccaagg cgcaaaccgc gtgcagcttt
    17701 tatgggcatc taccggcgtg aaaaacccgg cctatcccga cacactctac gttgacagcc
    17761 tgatcggcgc gcacaccgtc aacaccgttc ccgatgccac gctcaaagcc ttcatcgacc
    17821 acggcacagc caaagctacg ctgaccgaag gcgcggacga agcacaagct cagctcgccg
    17881 aaaccgccgc gctcggcatc gatgtcgaaa ccttggcggc acgtttgcag gaagacggtt
    17941 tgaaacagtt tgaagaagcg tttgaaaaac tgctcgcacc gttggtttaa acttttcatc
    18001 cccgatgccg tctgaaaggc tttcagacgg catcgcccgt ttgaccggac acagtgcggc
    18061 atatcaagga ttcaccctaa aaatattaat gggcaagaag ttaatgcacg acattaccca
    18121 ccaacacaaa aggaaataga atcatggcaa aagcactcga aatcatttca cccaatgaaa
    18181 tttattcaga tctgattttt aaggatccgg tacctcccca tacaatatat acagaattga
    18241 tgaaactggt tagtagaaat gcaagtaatg agagaatcgc agatagagcc ttcgattttt
    18301 tctcgtccgg ccttatggat ggttcagcta ccgaagagca atataatgcc ctatatgatt
    18361 taaccttact tgaagaaccc gggatggaac tgaataaaga tgagataatg gctttgatta
    18421 atagtcttaa ataaaggggc cgtaccggat atgtacacag gacgtttcgc ccccagcccg
    18481 accgggctgc tccacatcgg ctcgctgctg accgccgccg cttcctatgc cgatgcacgc
    18541 tcaaacggcg gcaaatggct ggtccgcatg gaagacctcg atccgccgcg cgaaatgccg
    18601 ggggcggcaa gccatatcct gcacacgctt gaggcattcg gattcgagtg ggacggagaa
    18661 gtcgcctatc agagccgccg ttacgccctg tatgaagaaa cactatgccg tctgcaaacc
    18721 gccggactgg tctatccctg ccattgcagc cgcaaagact ggcaggctgc ggcagtccac
    18781 ggcgcagacg gattcgtcta taacggacgt tgccgcaatc cgcagcaacg ccctgcactg
    18841 caaggcaaac agcctgcgtg gcgcatccgc gtccccgacc gcgacatcgg cttttcagac
    18901 ggcatcgtcg gcggttacgc ccaaaacctc gcccgcgaca tcggcgattt cgtcctgctt
    18961 cgtgcagacg gttactgggc ataccagctc gccgtcgttg ccgacgatgc cgaacagggc
    19021 gttacccaca tcgtccgcgg acaagacctg ctcgtttcca cgccgcgcca aatctatttg
    19081 cagcagtgtt tgggcgttcc gacaccgcaa tatgcccacc tgccgctgct gaccaacgca
    19141 caagggcaga agtggtcgaa acagacgctc gcccccgcat tggatttaaa ccgccgcgaa
    19201 caactcctcc gccaagtgtt ccgttacctc aagctgcccg aagcaccgga aaccgaccgc
    19261 cctgccgaac tgctcgactg ggcggtggca cactgggata tgggcaaagt gccgaaacac
    19321 gccattacca ccccctaaac accgcccgac aaacgcacaa atgccgtctg aagccttcag
    19381 acggcatttc acacatacag ccccgcccta ttcgcgcgta tttgcccgct caacctcttt
    19441 ccacgcttcg agaatcaggc tgccgtcgtt gtctttcaag agcgttgcgt cggaaagcat
    19501 acgccgccaa gtccgcgcac ctttcaaacc gtgcatcagc ccgaggctgt ggcggacgat
    19561 gtggcgcaag attgtgccgc gtccggcttg gatctgggct tggctgtatg tgtagaggca
    19621 ctgcaccaaa tcggcgtatt cgatcgggct gcgagtatcg ccgtaaaaca atctgtccca
    19681 ttcgcgcatc accatcgggt tgtggtacgc ctcgcgcccg accatcacgc cgtcaacgtg
    19741 ttgcaggtgt ccggcgattg cttcgttggt ggtgatgccg ccgttgatga tgatttccag
    19801 cccgggaaac tcctgcttga ggcggtaaac gtaatcgtat ttcaacgggg gaacgtcgcg
    19861 gttttctttg ggggaaagac cgtccagcca agcgttgcgg gcgtggacga tgaaggtttt
    19921 gcaggcggtt ttgtcgcgca gcgtgccgac gaaatcggca acggtttggt attcggtctg
    19981 cctgtccaca ccgatacggt gtttgacggt aacgggaata ccgaccgcgt cctgcatggc
    20041 gttgaggcag tcggcaacca gcccgacttc gttcatcaga cacgcgccga acgagccttt
    20101 ctgcacgcgc ggactggggc agccgcagtt gaggttgacc tcgttgtaac cgtatgcctc
    20161 ggcggctttg gcggctttcg ccaaatcgga cggatcgctg ccgcccagtt gcagggcgac
    20221 gggctgctcg ccttcgttga acatcaaaaa gcggtctttg tcgccgtaaa caatcgcgcc
    20281 ggcgttgacc atttcgctgt acagccaagt attgcgggta atctggcgcg ccatataacg
    20341 gtagtgcctg tccgtccagt ccaacatcgg ggcgacggac aggtggcgcg ggggaagggt
    20401 gtgtgtattg tcgttcattg tcgatatttt atcattgcgg attcaatttc aatccggaac
    20461 aaccttcatt tttggggaaa tccgcgtcat gaaagagtcc agcagtatgg ttaccggccc
    20521 gtcgttgcag agcgacacct gcatatgcgt gcggaaacgc cctgtttcga catgaatccc
    20581 gtgtccgcgc aacagttccg ccgttcgcag gtaaagctgc tgcgcctgtt ctgcaggtgc
    20641 ggcttgggaa aacgaaggcc gccgcccgct tgccgcatcg gcataaagtg taaactgcga
    20701 caccagcagc accgcgccgc cgacatcttt caaagacagg ttcaacttgc ccgcttcgtc
    20761 ttcaaacacg cgcaaatggg cgattttgtc ggcgatatag cgtgcatctt tttctgtgtc
    20821 gctatgcgtt acaccgagta gcacgacgaa tccgccgtcg attttgccgc aggtttccgt
    20881 gccggcttcg gacacgacat ccacctttgc acctaccgtt ttctgtatga ccgcacgcat
    20941 aagccgtcct ccttacctga acgtgcaatg ccgtctgaaa aggcttcaga cggcatcggt
    21001 atcggaaatg ccattacggt ttgacaccgt tttcttccaa aagggcatcg attttcaagc
    21061 gggctttttc ctgtctcaaa ccctgctcca attggtttct gaccaactcg aaaggttgcg
    21121 cgtcggggtt tttcccgacc tcgctgagtt tgaacagata atagcgttcg cccaatttga
    21181 ccggattgcg ggtaacgtcg ccccgattca tcgctgcaaa ctgcgaagcc agcggctcgg
    21241 gaagctgctg cgccataatg aaaccgtcaa aagcctgctc gtcgttcgga taacgcttca
    21301 tcagcccttc aaaagacagc cctttgagca ggagctgctg cgcctgacgc gcctcctctt
    21361 cggttgcgaa gctgacctgc tgcaatttga tcatgcggat ttgccgctca taaaactgac
    21421 gcagtgcgct ttcggaaacc gtttccgaac gttccagaaa acggacgtac tcctcggcat
    21481 aaaaagacgc ttcggcgatt ttaaagcggt tttggacatc cttatcctta tccaaacctt
    21541 ccttcaatgc cctgtttttc aaaacttcca aagtttgcag ccggcggacg gcatcgtttc
    21601 ggattgcctg cccgtccggt ttttgggact gctccgcatg ccggtctgcc tgctgcatga
    21661 tctgcgccac cagcgtatcc accaaagccg ggtcgatttc gggtgctttg gctgccgcaa
    21721 aacctgccaa cattgcagca ataactgcgg cagcggtttt tttctgtttc atggtattcc
    21781 ctgtcctgcg cggcggcaaa acgcctgtct gaacgtcttg ccgccatatc ccgttttcag
    21841 aattattttg caggtttgat gtttgccttg cccaacagtg caccgacggc acggtcaatc
    21901 cgttccgcct gaaggttgcc cgcaatctgt cctttcattt catcaaaaga aggcactttt
    21961 acctcgcggc tgtcgttgac ataataaacg ccgtagaaat cgccgttttt cagcggcgtt
    22021 gccgtaaatt cgcctttttt caagtcctta attgcctgat aaagcggcgg aacaccctgt
    22081 tccaaatctt tcagcggcac atatccgacc ggcgcaccgg tctgtttggt acggtcgttg
    22141 agggaatatt gtttcaagac ggcatcgaaa ccttttttcg ccttcaagtc ggcaaccgct
    22201 ttttttgcat tttcttcctt gtcggtcagg atttcgccca actggacttc ctgcgtacct
    22261 ttgtaaaaac cgctgatatt gtcatatgcg gcttttactt cctgctcgga aaccggttgg
    22321 gttttggcga tatgcaatgc gtatgcctcg ccgttcaagc catattttac cgcctgccaa
    22381 acggttttga aggacggttt cttgtcgtcg cccgactttt tcgcttcggc acgcaatttg
    22441 gcaagcgcat ttttaaactc tgccgaccgg tcgagtttca ggcgtttcac ttcctgtgcg
    22501 accacggtgt tgaccacttc gttttccagc agggattggc gcagttgcgg cgtgtcttcg
    22561 gcacggctgt tttccgcacg gaatgcggca acctgcgcat caatgacgga actgtcgatt
    22621 ttctgaccgt tgacggttgc cagcgtttgg gcaaacaggc tgccggaaca ggcaagcagt
    22681 gcaacggaag tcaggatttt tgctttcata atcttctctc tttaaatttt aaattgaaat
    22741 gttcgggcag acttcattca gtccgccgta tggaaatact cgtcaggggt agccgcccgg
    22801 atgccgagcg cgtgaatcat gccgcctgaa aacaaatctt tgagcagcga tttgaccgtt
    22861 ttctggcggt tcaggcggct tacgccttca aaacggccgc tgacgaccaa aacggcataa
    22921 tgtccgccgc cggtattgcc cgcgtgtcct ttgtgcagat ggctctcatc gccgatttcc
    22981 aacaccagcg gatcgagcgt ctgcaggcgt tcgcggatca aatcgacggc cggcattatt
    23041 tgaacaccgc tttaaacggc ttgatcagca cttcgctgta cacgcctgca tgaacatagg
    23101 gatcgtcttc cgcccacgcc tctgccgcat ccaaagactc gaactgcgcc acaatcaaac
    23161 tgcccgaaac gcggttggag tcctccggca aaggattcgg gcctgccgtc aacagccggc
    23221 cttccgactt cagcgtctcc agccgtttga ggtgttcggg acgtgccgcc atacgcgctt
    23281 catacacatt ctccccgtct gttgccagca acataaaata ttccacagtc aatcctcctt
    23341 tttcagacag gtactcagat aaataccctg aataataaag aaaacaagca tcagtgcagt
    23401 cgagccgaac attttatagt tgacccattg cgactcgaac cgggtaaaca caaaccagtt
    23461 ggcgataccc ataaaaatca ggaaaccgac ccacatatac gtcaatttcg cccatacggc
    23521 atccggaagc tgaatctccc tgccgatact cgccttcaag ccgtttttac cggcgaggtg
    23581 gctgccccac aggaacagcg cacccagcca aaacaaaacg ctcggcttcc acataatgaa
    23641 gcggctgtcg cccaaaacaa tggttgcgcc gccgaatacg acaatcagca ccagtccgac
    23701 ccactgcatc gtatccagct ttttatattt ccaatacaga aaagccgcct gaaccacacc
    23761 ggcaaccaag gcaaccgccg tagcggcaat catatttttg gtaacggtat aagtagcaaa
    23821 aaacaagatg acagacaaaa ggtcgctgac gaatttcata tccaaatgag aaagcaaatc
    23881 aaataaacgc taatcataca ggcagaaccc tgtttttgca cctgcaaaac caacaaattt
    23941 tgttttgatt gatgcaaata tgcacagatg tttttgaaaa aagatggaga tatgtcataa
    24001 tttgtaaaaa cggcgaatga ttgccgttta aaatgtggcg gcggtcggta cattcacata
    24061 ttaaatgccc gccggccgac tgccccgccg atgcccgatg atgacgaaat ttacagactg
    24121 tacgcggtca aaccgtattc agccgccaac ccacagggga tacatcttga aaaacaacag
    24181 acaaatcaaa ctgattgccg cctccgtcgc agttgccgca tcctttcagg cacatgctgg
    24241 actgggcgga ctgaatatcc agtccaacct tgacgaaccc ttttccggca gcattaccgt
    24301 aaccggcgaa gaagccaaag ccctgctagg cggcggcagc gttaccgttt ccgaaaaagg
    24361 cctgaccgcc aaagtccaca agttgggcga caaagccgtc attgccgttt cttccgaaca
    24421 ggcagtccgc gatcccgtcc tggtattccg catcggcgca ggcgcacagg tacgcgaata
    24481 caccgccatc ctcgatcctg tcggctactc gcccaaaacc aaatctgcac tttcagacgg
    24541 caagacacac cgcaaaaccg ctccgacagc agagtcccaa gaaaatcaaa acgccaaagc
    24601 cctccgcaaa accgataaaa aagacagcgc gaacgcagcc gtcaaaccgg cgtacaacgg
    24661 caaaacccat accgtccgca aaggcgaaac ggtcaaacag attgccgccg ccatccgtcc
    24721 gaaacacctg acgctcgaac aggttgccga tgcgctgctg aaggcaaacc caaatgtttc
    24781 cgcacacggc agactgcgtg cgggcagcgt gcttcacatt ccgaatctga acaggatcaa
    24841 agcggaacaa cccaaaccgc aaacggcgaa acccaaagcc gaaaccgcat ccatgccgtc
    24901 cgaaccgtcc aaacaggcaa cggtagagaa accggttgaa aaacctgaag caaaagttgc
    24961 cgcgcccgaa gcaaaagcgg aaaaaccggc cgttcgaccc gaacctgtac ccgctgcaaa
    25021 tactgccgca tcggaaaccg ctgccgaatc cgccccccaa gaagccgccg cttctgccat
    25081 cgacacgccg accgacgaaa ccggtaacgc cgtttccgaa cctgtcgaac aggtttctgc
    25141 cgaagaagaa accgaaagcg gactgtttga cggtctgttc ggcggttcgt acaccttgct
    25201 gcttgccggc ggaggcgcgg cattaatcgc cctgctgctg cttttgcgcc ttgcccaatc
    25261 caaacgcgcg cgccgtaccg aagaatccgt ccctgaggaa gagcctgacc ttgacgacgc
    25321 ggcagacgac ggcatagaaa tcacctttgc cgaagtcgaa actccggcaa cgcccgaacc
    25381 cgctccgaaa aacgatgtaa acgacacact tgccttagat ggggaatctg aagaagagtt
    25441 atcggcaaaa caaacgttcg atgtcgaaac cgatacgcct tccaaccgca tcgacttgga
    25501 tttcgacagc ctggcagccg cgcaaaacgg cattttatcc ggcgcactta cgcaggatga
    25561 agaaacccaa aaacgcgcgg atgccgattg gaacgccatc gaatccacag acagcgtgta
    25621 cgagcccgag accttcaacc cgtacaaccc tgtcgaaatc gtcatcgaca cgcccgaacc
    25681 ggaatctgtc gcccaaactg ccgaaaacaa accggaaacc gtcgataccg atttctccga
    25741 caacctgccc tcaaacaacc atatcggcac agaagaaaca gcttccgcaa aacctgcctc
    25801 accctccgga ctggcaggct tcctgaaggc ttcctcgccc gaaaccatct tggaaaaaac
    25861 agttgccgaa gtccaaacac cggaagagtt gcacgatttc ctgaaagtgt acgaaaccga
    25921 tgccgtcgcg gaaactgcgc ctgaaacgcc cgatttcaac gccgccgcag acgatttgtc
    25981 cgcattactt caaccttct
//
LOCUS       DAWFAD010000011        23739 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWFAD010000011 DAWFAD010000000
VERSION     DAWFAD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23739
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.011"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..801
                     /locus_tag="WGO49_000341"
     CDS             <1..801
                     /locus_tag="WGO49_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0305501.1"
                     /translation="RTPNIKYIYQDNQGLSEARNTGIKNSNGKYIVFIDSDDFINCQV
                     LLDFLSKDDIDMPDVVFLNAVKYDKGSVSYFGEDYQPEKILNQSKVEVLKGLCRFRKF
                     PGSAWNKIIKRELIIKEKLFFEKGIYSEDVEWSMRLFNAATTFSYLDGCYYYYRQGRE
                     DSITGTVSEKSIKSLLYILEKNAEMEFNRDISSYLYSFLSYEYLVLLFIMTSKNIACD
                     ADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFGVDITGRILKAIRGNI"
     gene            842..1372
                     /locus_tag="WGO49_000342"
     CDS             842..1372
                     /locus_tag="WGO49_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ0305502.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(1389..1520)
                     /locus_tag="WGO49_000343"
     CDS             complement(1389..1520)
                     /locus_tag="WGO49_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ0305503.1"
                     /translation="MPSKPEKRASDGIFACNSNADGQNQGASDTVIGHLVRVNLGLT"
     gene            complement(1539..3134)
                     /locus_tag="WGO49_000344"
     CDS             complement(1539..3134)
                     /locus_tag="WGO49_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ0305504.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(3241..3636)
                     /gene="hisI"
                     /locus_tag="WGO49_000345"
     CDS             complement(3241..3636)
                     /gene="hisI"
                     /locus_tag="WGO49_000345"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ0305505.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRILMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(3667..4434)
                     /gene="hisF"
                     /locus_tag="WGO49_000346"
     CDS             complement(3667..4434)
                     /gene="hisF"
                     /locus_tag="WGO49_000346"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ0305506.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTCPDLINEAAGFFGSQAIVAAVDAKAVNPENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(4447..5184)
                     /gene="hisA"
                     /locus_tag="WGO49_000347"
     CDS             complement(4447..5184)
                     /gene="hisA"
                     /locus_tag="WGO49_000347"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ0305507.1"
                     /translation="MLLIPAIDLKEGRCVRLRQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(5217..5855)
                     /gene="hisH"
                     /locus_tag="WGO49_000348"
     CDS             complement(5217..5855)
                     /gene="hisH"
                     /locus_tag="WGO49_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ0305508.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGCRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(5986..7488)
                     /gene="pta"
                     /locus_tag="WGO49_000349"
     CDS             complement(5986..7488)
                     /gene="pta"
                     /locus_tag="WGO49_000349"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ0305509.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(7673..8731)
                     /gene="fbpC"
                     /locus_tag="WGO49_000350"
     CDS             complement(7673..8731)
                     /gene="fbpC"
                     /locus_tag="WGO49_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ0305510.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(8752..10269)
                     /gene="fbpB"
                     /locus_tag="WGO49_000351"
     CDS             complement(8752..10269)
                     /gene="fbpB"
                     /locus_tag="WGO49_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ0305511.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(10337..11332)
                     /gene="fbpA"
                     /locus_tag="WGO49_000352"
     CDS             complement(10337..11332)
                     /gene="fbpA"
                     /locus_tag="WGO49_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ0305512.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(11583..12053)
                     /locus_tag="WGO49_000353"
     CDS             complement(11583..12053)
                     /locus_tag="WGO49_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HEZ0305513.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGREQELVLIW
                     RTEADIGNGSILQFVCNWSFPAAEKTCSVLKKIGAVHSAMLIHRAADALGKEIRHLQS
                     EGKNLKEMWDITQHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(12111..13487)
                     /gene="argH"
                     /locus_tag="WGO49_000354"
     CDS             complement(12111..13487)
                     /gene="argH"
                     /locus_tag="WGO49_000354"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ0305514.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRCRVNRMPLGAAALAGTTYPIQREITAELLG
                     FEQICQNSLDAVSDRDFAIEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELMRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMLA"
     gene            complement(13506..14375)
                     /gene="galU"
                     /locus_tag="WGO49_000355"
     CDS             complement(13506..14375)
                     /gene="galU"
                     /locus_tag="WGO49_000355"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0305515.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMIEVYERSGNSILGV
                     ETVEPSQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(14403..15002)
                     /gene="rdgB"
                     /locus_tag="WGO49_000356"
     CDS             complement(14403..15002)
                     /gene="rdgB"
                     /locus_tag="WGO49_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ0305516.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELVGKADKSCCYVCVLVFVLHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(14995..15228)
                     /locus_tag="WGO49_000357"
     CDS             complement(14995..15228)
                     /locus_tag="WGO49_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305517.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFFFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(15336..15869)
                     /locus_tag="WGO49_000358"
     CDS             complement(15336..15869)
                     /locus_tag="WGO49_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ0305518.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLADLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(15971..16429)
                     /gene="nudB"
                     /locus_tag="WGO49_000359"
     CDS             complement(15971..16429)
                     /gene="nudB"
                     /locus_tag="WGO49_000359"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ0305519.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            16640..16716
                     /locus_tag="WGO49_000360"
     tRNA            16640..16716
                     /locus_tag="WGO49_000360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16674..16676,aa:Pro,seq:ggg)
     gene            16874..18700
                     /gene="mafB"
                     /locus_tag="WGO49_000361"
     CDS             16874..18700
                     /gene="mafB"
                     /locus_tag="WGO49_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ0305520.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VSDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            18703..19167
                     /locus_tag="WGO49_000362"
     CDS             18703..19167
                     /locus_tag="WGO49_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305521.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            19356..19538
                     /locus_tag="WGO49_000363"
                     /pseudo
     CDS             19356..19538
                     /locus_tag="WGO49_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
     gene            19550..19837
                     /locus_tag="WGO49_000364"
     CDS             19550..19837
                     /locus_tag="WGO49_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ0305522.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            20333..21589
                     /locus_tag="WGO49_000365"
     CDS             20333..21589
                     /locus_tag="WGO49_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0305523.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNK
                     WQGTSKSGIKIEGFTEPNRTAYPIYE"
     gene            21620..22039
                     /locus_tag="WGO49_000366"
     CDS             21620..22039
                     /locus_tag="WGO49_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305524.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSTIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            22117..22200
                     /locus_tag="WGO49_000367"
                     /pseudo
     CDS             22117..22200
                     /locus_tag="WGO49_000367"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            22197..22439
                     /locus_tag="WGO49_000368"
     CDS             22197..22439
                     /locus_tag="WGO49_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ0305525.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            22517..22762
                     /locus_tag="WGO49_000369"
     CDS             22517..22762
                     /locus_tag="WGO49_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0305526.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            22765..23154
                     /locus_tag="WGO49_000370"
     CDS             22765..23154
                     /locus_tag="WGO49_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305527.1"
                     /translation="MWKIIKEDSDDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSYSLSKSKKNALTGIAFLRGIDVYDPPISKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            23372..23644
                     /locus_tag="WGO49_000371"
     CDS             23372..23644
                     /locus_tag="WGO49_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305528.1"
                     /translation="MPIDNIPFYIFDLIDLKEGVGDIYNILDFVPNSDLSKDQDDALT
                     GIAFLRGIDVYDPPVSKEKALKALKKHPEIYQRFQHFFPFVELPPL"
BASE COUNT         5737 a         5312 c         6682 g         6008 t
ORIGIN      
        1 cggacaccaa atatcaaata tatatatcag gataaccaag gattgtcaga ggcgagaaat
       61 accggaataa aaaattcaaa cggaaaatat atagtcttta ttgattcgga tgattttatt
      121 aattgtcagg tcttgctgga ttttcttagt aaagatgata ttgatatgcc ggatgtggtt
      181 tttttaaatg cagttaaata tgataaggga agtgtttcat attttggcga agattatcag
      241 cctgaaaaaa tactcaatca atccaaagtc gaagttttga aaggattatg ccgatttaga
      301 aaatttccgg gttcggcgtg gaataagatt ataaaaagag aattgattat taaagaaaaa
      361 ctgttttttg aaaagggaat ttattctgaa gatgtcgaat ggtcaatgag attatttaat
      421 gcggcaacaa ctttttctta tttggacggt tgttattact attatcggca gggaagagaa
      481 gattctatta cgggaactgt ttcggaaaaa agtataaagt cattattata tattttggag
      541 aaaaatgcgg aaatggaatt taatagggat atatcaagtt atctttattc ttttctttcc
      601 tacgaatatc tcgttttgct ttttatcatg acgagtaaaa atatagcgtg tgatgctgat
      661 ataaaaagaa gggcgtatca tttaaggttt atgctgttaa agtccaataa attgatatat
      721 aagctgatat tcccgataat cacattattc ggggttgata ttacaggcag gattttaaaa
      781 gcaatcaggg ggaatattta ataaatcctt taacactata catcttaccg aaggaggaaa
      841 aatgaacgca atccgaactt tccaaaaccg cacgcccgaa atccacgaaa gctgtatgat
      901 agacgaagcg tgcgtcgtca tcggcgaagt gtcgcttgcc gaagacgttt ccgtgtggcc
      961 gtgcgccgtg ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca gcaatataca
     1021 ggacggcagc gtcctgcacg tttcccacaa aaccgccgcc aaacccgaag ggtcgccgct
     1081 ggttatcggc gaagacgtta ccgtggggca caaagtgatg ctgcacggct gccgtatcgg
     1141 caaccgcgtc ctggtcggca tggggacgac ggttctggac gatgccgtga ttgaggacga
     1201 agtgatgatc ggcgcgggca gcctcgttcc gccgcgcaaa cgtttggcgg gcggctacct
     1261 ttatgtcggt tcgccggtca gacaagtgcg cgtgctgacc gatgaggaaa aagccttttt
     1321 gaaatattcc gccgcgcatt atgtgaagct gtcgaaacag tacgggatgt gaaatcacat
     1381 cggcgttcct acgtcagccc caaattcacg cggacgagat gcccgataac ggtatccgat
     1441 gcgccttgat tttgaccgtc tgcgtttgag ttgcaggcaa aaatgccgtc tgaagccctt
     1501 ttttcgggtt tagacggcat tttattgccg attgtttttt aaagtttgac cgaatgttcg
     1561 cgcgtttcgt ggaacacgat gtccggccaa cgttcttgcg tcaaccccaa attcacgcgg
     1621 ttgggggcga ggtaggcgag gttgccgccc gcgtcgatgg cgaggttgcc cgcgttggct
     1681 ttttcaaact ccgccagttt tttcttgtcg tcgcacgata cccagcgcgc cgaccagatg
     1741 gatgcgctgt cgaacacggc ttctacgccg tattcgttgg cgaggcgcga ggttacgact
     1801 tcaaactgca acacgccgac cgcacccaaa atcaaatccg cgccgctcat cggtttgaac
     1861 acctgtaccg cgccttcttc gccaagctgt tgcaaacctt tttgcagttg cttgattttc
     1921 agcgggtttt tgatgcggac gctgcggaac agttcgggcg cgaagaaggg aatgcctgta
     1981 aatgccagtt gttcgccttc ggagaagctg tcgccgattt ggatgttgcc gtggttcggg
     2041 atgccgataa tgtcgccggc gtaggcttct tcaaccagct cgcggtcgtg ggacatgaag
     2101 gttaccacgc tggaggcggc gatttcgcgg ttgatacgca ggtgtttcat tttcatgccg
     2161 cgctcgaatt tgccggagca gacgcgcaag aaggcaatac ggtcgcggtg tttcgggtcc
     2221 atattggctt ggattttgaa gataaatccg gaaaacttcg gctcgtccgg ctcgaccata
     2281 cgcacggtcg catcgcgtgg tttcggcgcg ggcgcccatt caatcaatga attgaggatt
     2341 tcctgaatac cgaagttgtt aatcgcagag ccgaagaata cgggcgtgag ttcgccggcg
     2401 aggaattcgt cgagattgaa ctcgttggaa gccgcctgca ccaattcgat ttcgtcgcgc
     2461 aactgttgga tttccaatgg aaagcgttgt tccaattcgg gattgtctat gcctttgatg
     2521 atgtcgaact cgtgcggcag gcgttcgccg cccgcttcaa agagatagat ttcgtcgttc
     2581 aggatgtggt atacgccttt gaagtttttg cccataccga tcggccaagt aacgggcgcg
     2641 cagcggattt gcaggatgtt ttccacttcg tccaagagtt ccagcgaatc gcgcacttcg
     2701 cggtcgtatt tgttcataaa cgtaacaatc ggcgtatcgc gcaggcggca gacgtttaag
     2761 agcttgatgg tttgcgcttc cacacctttt gccgcgtcaa tcaccattaa tgcgctgtcc
     2821 acggcggtta aaacgcggta ggtgtcttcg gagaagtctt ggtgtcccgg cgtgtccaag
     2881 aggttgacgg tgtggtcttt atagtcgaac tgcatcacgc ttgatgccac ggaaatgccg
     2941 cgctgcttct cgatttccat ccagtcggaa gtggcgaatt tgccggtttt tttgcctttt
     3001 accgtacccg cgctttgaat cgcgcctgaa aacagcaaga gtttttcggt tagcgtggtt
     3061 ttacccgcgt cagggtggga gatgatggca aacgtgcggc ggcggcgcac ttggtcgagg
     3121 atttcttggg acatggtttt ctttgcaaaa aggttcaggc cgcttttcag acggcccgga
     3181 cagtgtttga gacggcgaaa ttgtacaaaa aaatgcctga taattcaatg ttggaggcgg
     3241 tcagtgcgtg ctgccgtaaa tctctttttc gtctttcagg acggcatcgg cggtttccca
     3301 cgcgctgcca cgccagactt tgtaaaagca gctttctcgc ccggtgtggc aggcgatgcc
     3361 gccgttttgg gcgatgagca tcacaatggc gtcgccgtcg cagtcgaggc gcagttcgcg
     3421 gactttttgc gtgtgtcccg actcttcgcc cttcatccat tgtttttggc gcgaacggct
     3481 gtaatagtgg gcaaagccgg tttcgacggt tttttgcagg gcttcggcgt tcatccacgc
     3541 caccattaaa atacgtttgg tttcggcatc ttgggcgatg gcgcaaacca aacctttttc
     3601 gtcaaatttg acggcttcaa gcaggttttt atccatattt cctttcagac ggcatggtcg
     3661 gggcggtcag aggcgcactt cgatgccggc ttcgcgcata gcgcgtttgg cttcgcggat
     3721 ggcgatttcc ccgaaatgga aaacgccggc ggcaagtacg gcatcggctt tgccttcggt
     3781 tatgccttca atcaggtgcc ggacattgcc gaccccgccg gaggcgatga cggggatgtc
     3841 gacggcttcg gcaacggcgc gggtcagcgg caggttgaaa ccctgtttcg taccgtccct
     3901 gtccataccg gtaagcagga tttcgcccgc gccgcgtttt tgcatttcga tcgcccattc
     3961 caccgcatcc aaaccggtcg gattgcgccc gccgtgggta aagatttccc agcgtgtgtt
     4021 ttcggggttg acggctttgg catcgacggc ggcgacgatg gcttgcgaac cgaaaaatcc
     4081 ggcggcttcg ttaattaaat cgggacaggt aacggcggcg gtgttgatgc tgactttgtc
     4141 cgcgccggca ttgagcaggc ggcggatgtc ggcaacggtg cgtacgccgc cgccgacggt
     4201 cagcgggatg aagacttgtc cggcaacctc ttcgatgatg tgcaggatgg tgtcgcggtt
     4261 gtcggatgag gcggtgatgt cgaggaaggt caattcgtcc gcgccttcgc cgttgtagcg
     4321 tttggcggct tcgacggggt cgcccgcgtc gcgcaaaccg atgaagttca cgcctttgac
     4381 gacgcgtccg tcttttacgt cgagacaggg gatgatgcgt tttgccagtg ccataatcgg
     4441 atgcctttag tcgagggaat ctgccagttg ctgcgcttgg gcaaaatcga tgctgccctc
     4501 gtaaatcgcg cggccggtaa tcgcgcctgc tacgccatgt ttttcggcgg cacacagggc
     4561 gcggatgtcg tccaagccgg tcagtccgcc ggaggcgatg acgggaatgc ggacggcttg
     4621 ggcgagtttg accgtcgcgt cgatgttcac gccgctcatc ataccgtcgc gcccgatgtc
     4681 ggtgtagatg atgctgttga cgccgtcgtc ttcaaagcgt tttgccaaat caattacatg
     4741 atgcccggtt acggttgccc agccgtcgat ggcggccata ccgtctttgg catccagccc
     4801 gacaataatc ctgccgggga aggctttgca cgcctcgcgc acgaagtcgg ggtttttgac
     4861 cgccgccgtg ccgataatca cgtcgttcag acccaaatcc aaatattgtc cgatggtttt
     4921 caaatcgcgt atgccgccgc cgagctgtac ggggatgtct ttggcgacgg cggcaaggat
     4981 gtctttgatg gcgggcaggt tttgcggaac gccggcaaac gcgccgttca aatctaccag
     5041 atgcaggcgg cgcgcgcctt gtttgaacca gtgcagtgcg gtttcggcgg gcgaatcgga
     5101 aaagacggtc gcctcttcca tcagcccttg tctcaggcgg acgcagcgtc cttctttcaa
     5161 atcgatggcg ggtatcagca gcataatttt tctccttgtg cggggccgtg tccggcttac
     5221 cagtttaaaa agtttttcaa catcgtcagc ccggcatcgt ggcttttttc ggtgtgaaat
     5281 tgcgtggcga atacgttgtc tttgccgacg atgcaggcaa acggggacgg gtagtcgctt
     5341 tcgcccaata tggtttcggg attttcgggg gcgaaatagt agctgtggac gaagtaaaaa
     5401 cgcgtgtctt ggggaatatc tttaaacagc gggtggtttt gggtttggcg cacggtgttc
     5461 cagcccatat gcgggacttt cagacggcat ccctgcgggt cgcggaggtc gcgctcaaag
     5521 cgtctgactt tgccgccgaa ccagcccaag ccgtcggtgt ttccttcttc actgtggtcg
     5581 aataaaagtt gcgcgccgac gcagattccg aaaaagggtt tgttttttaa ggcatctttg
     5641 actgcctcgt ccaggccgcc gcgcgtcagt gccgccatac agtcgggcat cgcgccctgc
     5701 ccgggaaaaa tgactttgtc ggcgcgggac acgcgttcgg ggtcgccgct taaaaagatt
     5761 tcggtatttt ttccggcaag ctgccccgcc gtccggacgg atttcaatac ggaatgcagg
     5821 ttgcccatac cgtaatcgat aatggcgatt tgcatggctt cctcctcttt ttttgcaata
     5881 tggctgcgat tttaacaaac aaatgtgccg tgctgataaa aatgccgtct gaaaacggga
     5941 gtctgtcttc agacggcata gggtttaaac ccggaaagcc gtttgtcagc cttccatttg
     6001 ttttgcctga acggcagtca gggcgatggt gaacacgatg tcttccacca gcgcgccgcg
     6061 ggagaggtcg ttgaccggtt tgcgcaggcc ttgcagcagc gggccgacgc ttaagacgtt
     6121 ggcactgcgt tggacggctt tataggtgca gttgccggtg ttcaggtcgg ggaagaccaa
     6181 aacggttgcc tgtcctgcca ccgggctgcc cggagctttg gatttgccca cacccggcac
     6241 ggttgccgca tcatattgca gcgggccgtc gatggcgagg tcggggcgtt tttcccgggc
     6301 aagtttggtt gcttcgatga cggtatcgac atcggggccg ctgccggagt tgacggtgga
     6361 gtaggaaatc atcgccactt tcgggtcgat gccgaaggct tttgcggaat cggcagactg
     6421 gatggcgatg tcggcaagct gttgcgcggt cgggttcgga ttaaccgcgc agtcgccgaa
     6481 gacgaggact tggttgggca gcagcataaa gaatacgctg gacacgaggc ttgcgcccgg
     6541 tgcggtttta atcagttgca aagcggggcg gatggtgttg gcggtggtgt gaaccgcacc
     6601 ggataccaaa ccgtccacat cattttgcgc catcatcatc gtaccgagta ccacggtgtc
     6661 ttgcagttgc ttgcgcgcgt cttcgggtgt caggcctttg gatttgcgca gttcgcacat
     6721 cggctcgacg tattgttcga ccaatgaggc gggatcgatg atttccaaag agtcgggcag
     6781 gctgatgccg cgttctttgg caacggcttc gacttcttcg cgtttggcaa gcaggacgca
     6841 gcgggcaatg cctttttcgt ggcagatggc ggcggcttgg acggtgcggg gttctgcgcc
     6901 ctcaggcagg acgatgcgtt tgtcggcttg gcgggcgaag tcgatcaggt tgtagcggaa
     6961 ttgcgccggc gacaggcgtt ttgcttcgcg gcctgccaat acggatacgt ctttcagcgc
     7021 gtcgctcgaa ccgaagaagg tcaggccggt tttttcggct gccgcttcgg caacggaggc
     7081 tgccgcgccg tccacgacaa aaccttccaa tacgcccggc gcggcggcga agaactgttt
     7141 ggcaaggttc agccgatttg ccagttcgtc ggcatcggcg ttgtcggaac ggacggcgaa
     7201 gacggctgcc gcgtcaaggg acaatgccag ttcgacgttt ttgcctgcga ggtagatttt
     7261 gtcggcatcg ggcgcgatgc cttcgatgac gaggttggcg gcatcgagtg cggcaacttt
     7321 gccgaccagt gcgtcgaacc agtcgtcgct tttgccttgc gcgagcaggg tttcggcggt
     7381 tgcgtcaacg gcttggaaaa tttgtgcgtc cagtgctttt gcaaaggctt gtgcggcggc
     7441 ggaggcgtcc agtccggcag atacgggtac gatgagtact tttgccatga tatatccttt
     7501 cgtatgctgc ggtgtgcggc atatgtggtt ggaaggggcg gcatataggc agaaacggct
     7561 gcctgcgtgc cgtgcgtgcc gtgtttggct tgaggcgcgc aggttgaata tagcaaacaa
     7621 attctgtttc caacaagata aatatccgca ggcttgtgga tgctgccgcc tttcagaggg
     7681 tatttccggg gaagaacagg gcgggaccgt ccaaatggag gacggcggaa atgccgtctg
     7741 acagggtggg ggcggaaggg aggttgagcg tgaggacggt ttgtccgacc cggagactga
     7801 tttcggtatg ccgcgctttg ggcgtggttt tgagaaccac ggcgtgaatg gaggcggtgg
     7861 gtgcggaatg ggggtgaagg ctgaactgtt ccggacggat gagcagtgtg ccgcgtgtgc
     7921 ctgcgggtgc gccgctttga acgggcaggc ggcccaatcc gcaatcggcg gtgccgtcgg
     7981 cgttgagcgc ggcggggaac acgatgcctt cgccgataaa cagggcggca tcaaggtcgg
     8041 caggttgtcg gtacaattcg tgagggcttg cagtttggag gatgcgcccc tgtttcatca
     8101 cggcaatccg gtcggcgtat tgcagggctt cttcgcggtc gtggctgacg aaaacggcag
     8161 atttgccgtt ggcgcgcagg gcggcaatca tgtcttcgcg aatctggcgg cgcaactgtt
     8221 cgtccagcgc gctgaagggt tcgtccaaca aaatcagttc gggatcgggt gcgagggcgc
     8281 gggcgagggc gacgcgctgt tgctgtccgc ccgaaagttc gtgcggatag cgtccggcaa
     8341 gttcggaaat gccggtcaat tccaacatag cttcgatgcg ctgccgctct tgcgccgtct
     8401 tgcctttgcc gttgcccagc ccgtaggcgg tgttgcggta aacggtcagg tgggggaaca
     8461 gtacgccttc ctgtacgaca taacccaaac ggcgttcgcg gacggggagg ttggtatttt
     8521 tcgagaagat ggttctgccg gaaagcgaaa tttcgccaaa atcgggttgt tcaaaaccgg
     8581 caaggcagcg taaaagggtg gttttgccgc agccggacgc gccgacgata aagaggattt
     8641 cgcccgggtc gaggctgagc gaaatgtcgt ttaaaaccgg ggtgttttga aaacttttgg
     8701 acaggtgtcc gatgtgcagg gcggcggtca tggcggtact tcctcaagct gttatttgaa
     8761 ggcgtatttc ttcagcagga atacggggat gccggaaaat aataccagca tcagcgcgta
     8821 aggggtggcg gcggcgtatt gtgcgtccga tgtgtattcc caaacggcgg tggagagtgt
     8881 gtggacatcg tcggtggtca gcagcagggt ggcggtcagc tctttcatca gtttgaggaa
     8941 gacgagtgcg aatgcggcgg taatgccggg caggatggac ggcagtacca acgtcctgaa
     9001 aataaagaag tgtccgcgcc ccaatgttgc gccgacctgt tccatccctt tggggagttg
     9061 ttccaaggaa gtcctcaggg tggtttgcgc catcggcagg taaagcatga aataggcaag
     9121 gatgacgacg ataaaggttt ggtaaacggc aggggtgtag ttgatgctga aataaaccaa
     9181 ggatagggcg ataaccaaac cggggacggc gtgcagtaaa aacggcagcc tgtctatcca
     9241 aacggttaaa aaattgcgat agcgaaccga tgcccaaaca aggggcaagg cacataatat
     9301 agtcaaaatc gcacctaaag ccgatacgct taaggaacgg ataaaggcat caaatacgga
     9361 tacgagcgcg aatgtgccgg aagtgccgac catcatccaa tgtatcaata cgccaaaggg
     9421 gataataatg cccaaagtca acaagctgct taaaaaaaca atcgcgccaa tctgaccggg
     9481 cagtttgagg gttttgacgg gataaggacg ggcaacgcct ttgccgctgt ggtaaatctt
     9541 ggctttgccg cgaaatatgc tttctccaaa tacgacgatg ccgcacaccg ccattaaaac
     9601 agcggaaagc agggcggcgg tattgttgtt gtaggacatt tcgtattctt ggaaaatggc
     9661 ggtggtaaaa gtggggtagt tcaaaataga taccgcgcca aattcgacca gcatatgcag
     9721 ggcaatcagt aacacgctgc tgccgatggc gggtttgagc tgggggagga tggcggaaaa
     9781 aaaggtttgc aggcggcttt tgcccaagga caggctgact tcttcgtaag acaggctgat
     9841 gcgtttgagt gccgcctcga cgggcaggta ggcgagcggg aacgaggaca ggctcataat
     9901 catcactgtc ccccaaaagc cttcgacacg gaaggtcagg ctgatccagg tgaaacagct
     9961 gacaaatgcg gggatgcaca aaggcagggt gattgccgtc tgaaaaaagg ttttgccgaa
    10021 gaagcggtaa cgttggaaca aaagggcgca ggcaatgccc aaaacaatgg aaatcagggt
    10081 aacgcccgcc atcatcgtca aggtgttgga gagcaaatcc cacatacgcg ggcggaacaa
    10141 cagttcgacg gcgcggttga tgccgacctg ccacgaacgc atagcgacat ataaaaaagg
    10201 cagggtaagc ggtagggcaa tcagtaggat gaggccggta agccaaatgg gtattttttt
    10261 aggagacata gtgtttttta tcggcaaaac gggcggacag tataaatgtc cacccgtttg
    10321 acaatccgaa aacggcttat ttcataccgg cttgctcaag cagccgggtg gcgtgttctt
    10381 tttcggaaac agtggtggcg gacacttggg gtgcttccaa cttggcgatg ggttccaaat
    10441 tgaaagtgga taccacgtgc ggattcaaag gatattcggc acggacggcg gtcagggcgc
    10501 gctgtccttc cttgctggcg aggaaggcga cgaatttttt cgcctcatcc ttgttttggg
    10561 aggattttaa cacggctgcg ccggaatagg taacgagtgc gccgggatct ctgtggcgga
    10621 cgaaattcag gcgggtgtgg acattttgta cgcctttttc acgcgcaaaa gcgtgccagt
    10681 agtagttgtt gatgagggcg gcatcgattt cgccgttttc aaccgcttga agggcgacgg
    10741 agtttttagc gtaaggcttg ccgtattctt tcagaccttt gagccatttc aatgcggccg
    10801 cttcgccttt cagtttgacg atggcgacaa cctgttccaa gaacgcgccg gaagtggggg
    10861 cgtaaccgat gcggtttttc catttcggcg tggcgtaatt caggacggat ttttccaaat
    10921 ctttttcaga cagtttgcgg gtgtcgtaaa cgacgacgcg cgaacgtccg ctcagtgcca
    10981 cccagtcttt tttggcggca accggcacgc ccttgccgcg tgtttcgttg atggtggagg
    11041 cgggcagggg ctctaggagg ttggctgcgg aaagggtggc gagtgccggg atttgttcgg
    11101 aatagaatac gtcggcgggg cttcggctgc cttcttcttt gatttgaccg gcaagctggt
    11161 cgcctttggc gctgttgagt ttgactttga tgccggtagc ccgggtaaag gcatctgcaa
    11221 cggcttgtgc cgcttctttg tgttggccgt tgtacacggt aatgtctgcc agcgcggggg
    11281 ttgcggcggt cagggctgcg gcaagcagtg cgtatcggat agatgttttc atatcgattt
    11341 tctcctaatg aatgagagtg tacaccttgt taagacataa cggcgtgtag tgtattcctt
    11401 cttctttata aatgcaaata attatttttt aaatttgtta ttatccgatc cggttatggt
    11461 ttgttctgac ttgtattttt tccgtgcatc gcgcccgtaa ggcggaagcg gcgggcaatg
    11521 cgtggcggaa tgtgggtaaa ggcggcattt tgatttgtcg gaatgcttga gaacccctct
    11581 ctttaaaaca gcgtctgaac agggttgccg gaatagtatt gccatcccgg cagatacagt
    11641 ttgtcggggt cttgccaata ttgttcatcc agactgttcg gcagtgaggc ggttttgttg
    11701 tcgagatgtt gcgttatatc ccacatttct ttcaggtttt taccttccga ttggaggtgg
    11761 cggatttctt ttcccaatgc gtctgccgcc cgatggatca gcattgcgct gtgtaccgcg
    11821 ccgatttttt tcagaacgga acaagtcttt tcggcggcgg ggaaactcca gttgcagaca
    11881 aattgcagta tgctgccgtt accgatatcg gcttctgtcc gccaaatcaa aaccagctcc
    11941 tgttcccgcc cgtccatgct ttcgagtttg ccgtcatgct gttcaaaaag tttgtccacc
    12001 gcctgccaca tcatctgttc gaagcggtcg gcttgggtat ccgtatcggt catcgtgttc
    12061 ttgcctttta aaaatgccgt ctgaacattt cttcagacgg catttggggg ttaagccaac
    12121 atttcccgcc agcgtttcac ttggaagcgg acttgttccg gcgcggtacc gcccaagtgg
    12181 ttgcgggcgt ttaagctgcc ttcgggtgtc agcacgccgt aaacgtcgtc ggcaatcaaa
    12241 tcgctgaaac cttgtaagac ttcgagcggc agttcgctca aatcgacgcc cgcttggtcg
    12301 gcgtggcgca cggcttgggc gacgacttcg tgggcatcgc ggaaaggcat gccttttttg
    12361 accagataat ccgccaagtc ggtggcggta gcgaagccct gcatcacggc ggcgcgcata
    12421 ttgtcgggtt tgacggttac gccgcgcatc atatcggcgt aaatccgcaa cgtgtcgata
    12481 agcgtgtcgg cggtgtcgaa caagggttct ttgtcttcct gattgtcttt gttgtacgcc
    12541 aagggttggg atttcatcag ggtaatcaga ccgataaggt gtccgatgac gcggccggat
    12601 ttgccgcgca tgagttcggg cacgtcgggg tttttcttct gcggcatgat ggacgaacct
    12661 gtgcagaaac ggtcggcgat gtcgataaag ccgaaacgcg ggctcatcca caaaatcaat
    12721 tcttcagaca ggcggctcag gtgaaccata accagcgagg cggcggctgt gaactcaatg
    12781 gcgaaatcgc ggtcggatac ggcatcgagc gagttctggc agatttgttc aaagcccaat
    12841 agctcggcgg tgatttcgcg ctgaatcggg taggtcgtcc cggcaagggc ggctgcgccg
    12901 agcggcatac ggttgacgcg gcagcggcag tccgccatcc gttcgttatc gcgtccgagc
    12961 atttcgacgt aggcgagcat atggtgtccg aagctgacgg gctgggcgac ttgcaggtgg
    13021 gtaaagcctg gcatgacggt ttcggcgttt tgttccgcca aatccagcaa tgccgtctga
    13081 aggctttgaa tcaggctttg tataacggta atctggtcgc gcagccacag gcggatgtcg
    13141 gtggcgactt ggtcgttgcg gctgcggccg gtgtgcaggc gtttgcccgc gtcgccgatt
    13201 ttgtcggtca ggcggcgttc gatgttcata tggacatctt ccaaatcgga cgaccattcg
    13261 attttgccgc tgcggatttc ttcgaggatt tccgccatac cccggcggat gtccgccaaa
    13321 tcgccttcgt ccaacacgcc ggtttctttc agcatttgcg cgtgtgccag cgagccttgg
    13381 atgtcccatt cggcaagccg tcggtcgaaa ccgatggagg cggtgtattg tttgacgagt
    13441 tcggaaacgg gttcgttgaa acgtccggac caggttttgt cgtgcataag gattccttga
    13501 tggggttatt cggtgcggta tttttccaaa agccggcgga agggttcgcc ggtttcggga
    13561 tgtttcagac cgtaggcgac ggtggcttcg aggtagccca gtttgctgcc gcagtcgtag
    13621 cgcgtacctt caaaggggtg cgccaggaca aattcgtgat cgagcagctt ggcgatgccg
    13681 tctgtaagct ggatttcgtt gcccgcgccg cgcggaagat tggttaagag gtcgaaaatg
    13741 cgcggggtga ggatgtagcg tccaacaacg gcaaggttgg agggcgcgtc ttcgggcttg
    13801 ggtttttcga caatgccgat aatgcgttgg aactgtttga gctgttcggt ttcgacgatg
    13861 ccgtatgagc cggtttgcga cggttcgacg gtttctacgc ccaaaatgct gttgccgctg
    13921 cgttcgtaca cttcaatcat ttgtttgagc gcgccttttg gggcatcgat taaatcgtca
    13981 gccaaaatca cggcaaaggg ttcgtcgccg atggcggcgc gggcgcacaa gacggcgtgt
    14041 cccaagccca gtgcttccgc ctgacggatg tagaggcagg taatgttcgg cggcaggatg
    14101 ttgcggacgt gttccaacaa tttgtcttta tggcgcattt ccaactcggt ttcgagttcg
    14161 tatgccttgt cgaaatggtc ttcgatgctg cgtttgttgc gtccggtaac aaacaccatt
    14221 tccgtgcagc cggcttccac ggcttcttct acggcgtatt ggatcagcgg cttgtcgacg
    14281 atgggcagca tttctttcgg gcttgccttg gtggcgggca ggaagcgtgt tcccatccct
    14341 gcgacgggga aaacggcttt ccgtatgggt ttcattcttt ttcctttgta ttgttttgat
    14401 gtttaaaggg cgagtttgcg taagagttcg gcaagtgcct gcgcgcggtg gctttcgcgg
    14461 tttttgacct ccgaatccaa ttcggcggcg gttttgccgt gtccgggcag ataaaaatac
    14521 gggtcgtaac cgaaaccgtt ttcaccggcg ggaacgccgt tccattgtcc gtgccatacg
    14581 ccttcggcaa taatcggacg cgggtcgtct ttatggagga caaaaaccaa tacgcagacg
    14641 tagcaacagc ttttgtcggc tttgccgaca agttcggcgg caagtttcag gttgttggcg
    14701 gtatcggatt tgggattgtc gcccgcgtaa cgtgcggaat ggatgcccgg cgcgccgttt
    14761 agggcggcgg cacagatgcc gctgtcgtcg gcaagcgcgg gcagcccgct gtattcggcg
    14821 gcatggcgtg cttttgccag cgcgttttcg acaaaggtgg gatagggttc ggggcattcg
    14881 ggtatgccga atgcggattg cggcaatacg gtgatgctgt aaggtttgaa taagttgccg
    14941 aattcttcga gcttgccggc attgccgctt gccaaaacga ttttttccgg tttttcagac
    15001 atagcggttt tcctttgcgg cggattgggc ggcgcgtagg gatttgtgcc gcaggtagag
    15061 ggcgaggctg ccgatttgtc cgaacagtgc gccgaatgcg aaaaaaaagg aggcgacggc
    15121 gaaggctttg ctgccgacgg tcagcagata agcacccaag aggacgagca gcataaatgc
    15181 cagtgtgaag agggcaacag acaacagata gatttttcgg cggttcatgg cgttcggtcg
    15241 gaaacggtat gttcggatta tagccgattg ggacggtatt ccctagagct tggaaaaatg
    15301 ccgtctgaag cggcgttcag acggcaggga gagcgttatg cctgtttgtt ccaacgttcg
    15361 atggattctt tgatgacttt tttcgcttct tccgcatcgc cccacgattc gactttggtc
    15421 gtacccgctt ttttcaggtc tttgtagtgg ttgaagtgga actcgatttg tttgatgagc
    15481 tgttgcggca aatctgccaa agttttgtag gcgttgccgt tgttgcggtc gtcggcagga
    15541 acgcagacga ttttgtcgtc cacttcgccg tcgtcaacga atttcatcac gccgataacg
    15601 cgcgcttcca agaatacgcc ggttgccaaa ggttgttcgg taacgagcag cacgtccaat
    15661 tcgtcgccgt cttcgtccaa agtttgggga atgaagccgt agttggtcgg tttggcgaag
    15721 atggcgggtt cgacgcggtc gagttggaat gcggcgagtt tgcggttcca ctcgattttg
    15781 tggttgctgc cggcggggat ttcgttgacg acgttgatga tgccgccgtc cacatcgccg
    15841 ggggtcagga tttggttgaa gtctgccatt tggtttcctt tgtttgggaa agtttgaagt
    15901 ttgaaagtat agcacaaacg tccggctgaa aatgcgcccg atgccgtctg aaagggtgta
    15961 cgggcgcgtg ttaccgtttg cccaaaaacc tgcccagttc cagaatcgcg cgcctgttgg
    16021 acggggaaaa cactttttcc gccgcttctt ccaagccgaa ccagccgtag gagacgtgtt
    16081 cttcgggttg cagggcgatg ggcgtatcac gcgggatttc ggcagagaag aggtgttcgc
    16141 ggttttcaaa cacgcctttt ggatagcggt gccgccagtg gtggtagatt tcgtaaaccg
    16201 tgctgtcgtg ccagtcttga agctgcccgt ccgccagcag gatgccggtt tcttcccaaa
    16261 cttcgcgcct tgccgtttgg gcgacggttt cgcccggttc gaggctgccg gttaccgact
    16321 gccaaaatcc ttccggatgc gtgcgttcga tgagcaggat gccgccgtcc ccgctataaa
    16381 ggacgaccag tgcggaaacg gggtatttga gcggttttgc catcggcatc tttcggcggg
    16441 ctgcggtaat gaaggggcgg attatagcaa acgccgcacg ttatggcgtt tatccttttc
    16501 cgtatccttt tttctgcacg gatgggacgc gccggtgttt gccggtaaat tttccgttgt
    16561 gtcaaaaaga taagggcggt tgtgatttta atgcttgcca aagcgtcggg cggaaactat
    16621 aatccgaaac ttatcgagtc ggagtgtggc gcagtctggt agcgcacttg catggggtgc
    16681 aaggggtcga aggttcgaat cctttcactc cgaccaaaaa ttccgaaagc cgctttcaaa
    16741 agcggctttt ttgccgtccg tatgattatg atgtagagta cgcggcgaca gacattcaaa
    16801 tgccgtctga aaaccgttca gatggcatct ctttatctta gtttcattcc gtaccatctt
    16861 aaggaacatc aaattgggca tttcccgcaa aatatccctt attctgtcca tactggcagt
    16921 gtgcctgccg atgcatgcac acgcctcaga tttggcaaac gattctttta tccggcaggt
    16981 tctcgaccgt cagcatttcg aacccgacgg gaaataccac ctattcggca gcagggggga
    17041 acttgccgag cgcagcggtc atatcggatt gggaaaaata caaagccatc agttgggcaa
    17101 cctgatgatt caacaggcgg ccattaaagg aaatatcggc tacattgtcc gcttttccga
    17161 tcacgggcac gaagtccatt cccccttcga caaccatgcc tcacattccg attctgacga
    17221 agccggtagt cccgttgacg gatttagcct ttaccgcatc cattgggacg gatacgaaca
    17281 ccatcccgct gacggctatg acgggccaca gggcggcggc tatcccgctc ccaaaggcgc
    17341 gagggatata tacagctacg acataaaagg cgttgcccaa aatatccgcc tcaacctgac
    17401 cgacaaccgc agcaccggac aacggcttgc cgaccgtttc cacaatgccg gtagtatgct
    17461 gacgcaagga gtaggcgacg gattcaaacg cgccacccga tacagccccg agctggacag
    17521 atcgggcaat gccgccgaag ccttcaacgg cactgcagat atcgtcaaaa acatcatcgg
    17581 cgcggcagga gaaattgtcg gcgcaggcga tgccgtgcag ggtataagcg aaggctcaaa
    17641 cattgctgtc atgcacggct tgggtctgct ttccaccgaa aacaagatgg cgcgcatcaa
    17701 cgatttggca gatatggcgc aactcaaaga ctatgccgca gcagccatcc gcgattgggc
    17761 agtccaaaac cccaatgccg cacaaggcat agaagccgtc agcaatatct ttacggcagt
    17821 catccccgtc aaagggattg gagctgttcg gggaaaatac ggcttgggcg gcatcacggc
    17881 acatcctgtc aagcggtcgc agatgggcgc gatcgcattg ccgaaaggga aatccgccgt
    17941 cagcgacaat tttgccgatg cggcatacgc caaatacccg tccccttacc attcccgaaa
    18001 tatccgttca aacttggagc agcgttacgg caaagaaaac atcacctcct caaccgtgcc
    18061 gccgtcaaac ggcaaaaatg tcaaactggc agaccaacgc cacccgaaga caggcgtacc
    18121 gtttgacggt aaagggtttc cgaattttga gaagcacgtg aaatatgata cgaagctcga
    18181 tattcaagaa ttatcggggg gcggtatacc taaggctaag cctgtgtctg atgcaaaacc
    18241 gagatgggag gttgatagga agcttaataa attgacaact cgtgagcagg tggagaaaaa
    18301 tgttcaggaa ataaggaacg gtaataaaaa cagtaacttt agccaacatg ctcaactaga
    18361 gagggaaatt aataaactaa aatctgccga tgaaattaat tttgcagatg gaatgggaaa
    18421 atttaccgat agcatgaatg acaaggcttt tagtaggctt gtgaaatcag ttaaagagaa
    18481 tggcttcaca aatccagttg tggagtacgt tgaaataaat ggaaaagcat atatcgtaag
    18541 aggaaataat agggtttttg ctgcagaata ccttggcagg atacatgaat taaaatttaa
    18601 aaaagttgac tttcctgttc ctaatactag ttggaaaaat cctactgatg tcttgaatga
    18661 atcaggtaat gttaagagac ctcgttatag gagtaaataa aaatggcaat ttggaattat
    18721 cggtactacc ttatcccatc agctgctatc agaaataaat ttaatgcaaa taaagatatc
    18781 atacttgatg agtatcgatc taatggtttt cagaatttta atgagaataa aagttttgaa
    18841 aattacttta tcgataatga tgttatatta ttatcaataa taaatgaagc aaaaaaacag
    18901 cttaaattga aagaatcttg ggataaagac gcaatcatgt tttgtgataa ttttggtaat
    18961 agtcttaccg tttggccaga tgatatagag tgcgaacttg atttaagatt tgattatact
    19021 aaatttattc agaaaaccat tgattgggca ataaaatata attgtctact tgtaatagaa
    19081 aaaacaggaa atgtagtttc ccctaatata aataatctga tgtatgaaat aaaagcatat
    19141 ttggaaagca agccgtggcc catatgaaac ctaaactcaa caagtaggat gtgtgcggaa
    19201 cgcacgtatg cggttctcaa ggtttgagct aagaggccgt ctgaaaacag aaaaactgtt
    19261 tcagacgacc tttcttttaa ccagttgcca cagcaaccgg acaaaagcag cctacctcca
    19321 catccatata ggcaatacag gggagatatt ttgtaaattc tacgaatatt ttacctgcta
    19381 aacagggtag gatatggtat gaagcgaaca ttggcttaat aaacactatg tcaagatcga
    19441 atcaggctgg tactagattg ttgtattcca attatggatt gctatatata acaactgatc
    19501 attatatctc tgcaacacgg tttgtagctt ggaaatagga gtataactta tgcaattaga
    19561 gattatcggt agtaaaattt atacggaaca agattttcat aatcaaattt caaaaatatt
    19621 ttctatacaa gattattatg ggaacaatct tgatgcttta tgggatttat taagcacaaa
    19681 tgtagaacga ccgattactt tggtatggaa agatgctatg ttctcaaaaa atcaattaga
    19741 aaatatattt attgaaatcg taaatgttct agaaagagtt aagaaacaag atgaggatta
    19801 tggattcgaa gaaaaattta attatatttt agagtaagta aaccctaatt acattacgta
    19861 cacaggctta aaactcccca gagccaatta agcaagccgt aacccatata aaacttaaac
    19921 tcaacaagta gcatgtgtgc ggaacgtacg catgcggttc ttaaagtttg agctaagagg
    19981 ccgtctaaaa acagaaaaac cgtttcagac ggtctttgtt taacgccacc gatccagcgg
    20041 gttacaaagc gcagtcaatg ccgctgcgcc ttatgcctcc gaagcaatag gcagaacatt
    20101 tggacacggt gaaaacaaaa acgaaaccgc ccaagccgtc ggacatttcc ttttaggagc
    20161 agctattgcc cgcgtcaacg gtggtaattt tgctgccggc ggctcggcag cagttgcagc
    20221 tgaaaaggcg gcggaacatc ttgcccaaca gtataacgac ggtaaaaccg caatcgatcc
    20281 gcaaacaggc gagttcaatg ccaacctgct gccggaacat atcaaagagg aaatcaaatc
    20341 aaagagcggg gtgattgcat cgctgacggg cgcggccgtg ggcggcacgc cggtagatgc
    20401 gcaaaccgga ggtgcggtcg gacagaatgc ggtggaaaac aacctctatc tgacatcgga
    20461 agccttaaag aaggacaagc agacagctcg taaaatttat tccgtcataa aagagcaagt
    20521 caagcatgaa tgcagttcca caggaagaat taccgaatgt cgtcaaaata taggacgcat
    20581 tatcgaattt acccaagaca aacgctttga cagtaggttt aaggacttaa aaaaagaatc
    20641 cttatattac ctaaataaac atcctgattt agtagcctct tatttgaagg ctgaatacga
    20701 aaagctggat agggaagaca aaagtatcct gcaccgctac atctcacccg gggctgaaat
    20761 cgtttcgggc agtttggggg ttgttctttc aggagtagcc ggaggcggat cttgtgccga
    20821 gactttcggc ttaggctgtg ccgccgcttt ggttggtgta acgtcttcct acgatcatgt
    20881 cattactggg acgaaaaact tcggaaaaaa agccagcgag caacgaccga cgattgcggt
    20941 tcaggccttg aagcagttgg ggctgtcgga gcaggctgcg gaatatgttc agttctctat
    21001 agatttgttc agtgtgggta aatcgggggg cggtatacct aaggctaagc ctgtgtttga
    21061 tgcgaaaccg agatgggagg ttgataggaa gcttaataaa ttgacaactc gtgagcaggt
    21121 ggagaaaaat gttcaggaaa cgagaagaag gagtcagagt agtcagttta aagcccatgc
    21181 gcaacgagaa tgggaaaata aaacagggtt agattttaat cattttatag gtggtgatat
    21241 caataagaaa ggcacagtaa caggagggca tagtctaacc cgtggtgatg tacgggtgat
    21301 acaacaaacc tcggcacctg ataaacatgg ggtttatcaa gcgacagtgg aaattaaaaa
    21361 gcctgatgga agttgggagg tgaaaacgaa aaaaggtggg aaagtgatga ccaagcacac
    21421 catgttccca aaagattggg atgaggctag aattagggct gaagttactt cggcttggga
    21481 aagtagaata atgcttaagg ataataaatg gcagggtaca agtaaatcgg gtattaaaat
    21541 agaaggattt accgaaccta atagaacagc atatcccatt tatgaatagt aatatttatg
    21601 aaaaattagg agattaatga tgaaaagaat taagtgcttt tgtgataaat ttccatcagg
    21661 agatacattt agaatgtgta tcattctgga tgactatgat aatagggttg attattatgt
    21721 aggaatatat gattacatta cgtctacctt aatgagcgat atttactatc gatccacgat
    21781 tgatgagcat ttcaagatta tagaattaat agaaaataat ccaaatgaaa tttatgatga
    21841 tggcggtggt caacaatttt gcctagaatt tcatcatgat aaggtcattt tttaccacaa
    21901 tgaatttgat gaagaagatg gttatccagt attaagctgt tcgctgcata cttttaaaac
    21961 tgctttaatt gcttggaatg cttttttgca attgcctaaa agtattcatt cggtggtgga
    22021 gactgtgatt gaggaataag cataattagc ttaatgaata gaatcagcga tatagattgg
    22081 actgcaaatc cacgcttata cgctgtgcca tgattaacag gaagagagca acttggatgg
    22141 gctagtaggg atggtaagca tttaaatata tcaattgatg gaaagattac acacaaatga
    22201 ctaatttaaa attagatttc tactctgaag ttattataaa agattcttgc cctaatgatt
    22261 tgttagaaaa tggtgagact attaaaggaa aaaaaggagt ggtattaggt ataagcgaag
    22321 aagatggtat aatctatggc tatactattt tactttttga tataaaatat tgtatatata
    22381 tagataagaa atatattata ccgactggaa aaaaattctc tcgcgatgat ttttattagc
    22441 taatttaagt aatttaagca agttttttag atactgaaac tggtaattat gtttatgtaa
    22501 gcataaggag ttcagattga aggattctac agggcaaatg aggctggcaa ccaaggattt
    22561 ggcggaagcc attaaacgag gagaagtacg tagttctgct tttacaacaa agcaactaaa
    22621 ggcaatcgaa aaaggagaat ctaaaatacc ggattacact tggcatcatc atcaagacac
    22681 tggaaggatg caattgatta atgaagactt gcatcatgat accggccata tcggttggag
    22741 agcgatgagt aaaggaaagt aactatgtgg aaaatcataa aagaggatag tgatgattta
    22801 ggatttgcaa ttaaatgctt attctctcaa tctattgatt taaatgaatt caagttatgg
    22861 attgaacaag taatacgcga tatgcccatc gaggacatcc ctttttatat ttttgacttg
    22921 gcggattttg atgggggaat tgccgatatt gacaatattg taggttttgt ttcgagttac
    22981 agcctatcaa aatcgaaaaa aaatgccttg acaggcattg ccttcttaag ggggatagat
    23041 gtctatgatc cgcctatttc aaaagaaaaa gcattaaaag ccttaaagaa acatcctgaa
    23101 atttatcaga agtttcagca tttctttccg tttgtagaac tgcccccgct ttaaacagtc
    23161 aaaatgccgt ctgaaatgat attcggcttt cagacggcat ttttgacaca aagcaggtaa
    23221 ccaaaagagc gtttgacggc aaaggaagat aattatgtga aaaattacga aagagcaagg
    23281 tcaagattta agttttgcaa catattgctt attatcggca gcaattaatt tgcaagaatt
    23341 taaattatgg ttggagaagg ttgtcttaga tatgcctatt gataatatac ctttttatat
    23401 ttttgattta attgatctta aagaaggagt aggagatatt tataatattt tggattttgt
    23461 tccgaattca gacttatcta aagaccaaga tgacgcatta acaggcattg ccttcttaag
    23521 ggggatagat gtctatgacc ctcccgtttc aaaagaaaaa gcattaaaag ccttaaagaa
    23581 acatcctgaa atttatcaga ggtttcagca tttctttccg tttgtagaac tgcccccgct
    23641 ttaaacagtc aaaataccgt ctgaaagcca tttccgccgc tcagacggca ttttcgcccc
    23701 ttttgtttac aaacccttaa aatccattta cactcaaac
//
LOCUS       DAWFAD010000012        22770 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWFAD010000012 DAWFAD010000000
VERSION     DAWFAD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22770
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.012"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(67..906)
                     /locus_tag="WGO49_000372"
     CDS             complement(67..906)
                     /locus_tag="WGO49_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0305529.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1191..1266
                     /locus_tag="WGO49_000373"
     tRNA            1191..1266
                     /locus_tag="WGO49_000373"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1224..1226,aa:Arg,seq:ccg)
     gene            complement(1331..2524)
                     /locus_tag="WGO49_000374"
     CDS             complement(1331..2524)
                     /locus_tag="WGO49_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0305530.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAATPVPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFESAGFEVGTYPYYDPAAVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2530..3633)
                     /gene="trmA"
                     /locus_tag="WGO49_000375"
     CDS             complement(2530..3633)
                     /gene="trmA"
                     /locus_tag="WGO49_000375"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ0305531.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     TVERAALFDQFPFTHHIESGVLLKKKILGKSKR"
     gene            3776..4876
                     /gene="aroC"
                     /locus_tag="WGO49_000376"
     CDS             3776..4876
                     /gene="aroC"
                     /locus_tag="WGO49_000376"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ0305532.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRRETDQVEILSGVFKGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            4967..5392
                     /locus_tag="WGO49_000377"
     CDS             4967..5392
                     /locus_tag="WGO49_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ0305533.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(5466..7451)
                     /gene="parE"
                     /locus_tag="WGO49_000378"
     CDS             complement(5466..7451)
                     /gene="parE"
                     /locus_tag="WGO49_000378"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ0305534.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7518..8042)
                     /locus_tag="WGO49_000379"
     CDS             complement(7518..8042)
                     /locus_tag="WGO49_000379"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ0305535.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLSQHVKIVGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQLSGNR"
     gene            8207..9502
                     /gene="serS"
                     /locus_tag="WGO49_000380"
     CDS             8207..9502
                     /gene="serS"
                     /locus_tag="WGO49_000380"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ0305536.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLEAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLQHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDETKTTQYLIPTAEVTLTNTVADSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYEALEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9580..10578)
                     /locus_tag="WGO49_000381"
     CDS             complement(9580..10578)
                     /locus_tag="WGO49_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0305537.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAHDPFCNPEAEKIGGRYVDLDELYAQSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(10703..11779)
                     /gene="prfA"
                     /locus_tag="WGO49_000382"
     CDS             complement(10703..11779)
                     /gene="prfA"
                     /locus_tag="WGO49_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ0305538.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYRLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGELDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PADLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11869..12639)
                     /locus_tag="WGO49_000383"
     CDS             complement(11869..12639)
                     /locus_tag="WGO49_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0305539.1"
                     /translation="MRLTDTHCHLADSVLRGNLAQVLAEARQAGVWRFIVPATRPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLETMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANILRIAEIAAQIRGTGAAEIAAITERNADSLLRP"
     gene            complement(12642..13634)
                     /locus_tag="WGO49_000384"
     CDS             complement(12642..13634)
                     /locus_tag="WGO49_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ0305540.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDAADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAQALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSEGWGADEVKHELQRLGLV"
     gene            complement(13701..14384)
                     /locus_tag="WGO49_000385"
     CDS             complement(13701..14384)
                     /locus_tag="WGO49_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0305541.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14573..15907)
                     /gene="glmM"
                     /locus_tag="WGO49_000386"
     CDS             complement(14573..15907)
                     /gene="glmM"
                     /locus_tag="WGO49_000386"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ0305542.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEVELEIEAKIDGEMKTLPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLAQLNQRSWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIQKGQKWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16037..16888)
                     /gene="folP"
                     /locus_tag="WGO49_000387"
     CDS             complement(16037..16888)
                     /gene="folP"
                     /locus_tag="WGO49_000387"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ0305543.1"
                     /translation="MVGCVWQAGRFEIGLDKPKIMGIVNLTPDSLSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRPGADYVSPEEEWARVESVLAEVAGWGVPVSLDTRH
                     TVVMEKALALGGIDIINDVAALTDEGALELLACQADTGICLMHMQGLPKNMQINPKYQ
                     DVVGEVARYLKARAAECIAAGIAPQRITLDPGFCFGKTLQHNITLMRHLPELMAETGY
                     PLLIGVSRKSMIGELTGETDAAARGHGSVAAALAAVARGAKIVRVHDVKATADALKAW
                     EALGINL"
     gene            17163..19277
                     /locus_tag="WGO49_000388"
     CDS             17163..19277
                     /locus_tag="WGO49_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ0305544.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAIGLHASVYRIFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKGHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(19342..20820)
                     /gene="ubiD"
                     /locus_tag="WGO49_000389"
     CDS             complement(19342..20820)
                     /gene="ubiD"
                     /locus_tag="WGO49_000389"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ0305545.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRIAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYGYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(20840..21355)
                     /locus_tag="WGO49_000390"
     CDS             complement(20840..21355)
                     /locus_tag="WGO49_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ0305546.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPYPYNQLSEEERIAYQAECGGDF
                     CIIRYPDQTDRFIRAVLLIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENVVYF
                     GMICNRLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGSEHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(21362..21862)
                     /locus_tag="WGO49_000391"
     CDS             complement(21362..21862)
                     /locus_tag="WGO49_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305547.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEK
                     AVRYTRRVTQVWCVFFIANGTLAALLARLGRYDWWAVYTGVIAYVLMGMPFAGEWLYR
                     KLVLKV"
     gene            complement(22094..22663)
                     /locus_tag="WGO49_000392"
                     /pseudo
     CDS             complement(22094..22663)
                     /locus_tag="WGO49_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            22703..>22770
                     /locus_tag="WGO49_000393"
     CDS             22703..>22770
                     /locus_tag="WGO49_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0305548.1"
                     /translation="MLPSKYEDNTLQIKEKSTERTAP"
BASE COUNT         5194 a         6487 c         5946 g         5143 t
ORIGIN      
        1 attcccataa aaaaacggag cagatacctg ccccgttttt atttaatccg aaattttaat
       61 ctaaatttag aattttgcac cggattggtt tgccatatag tcaacagccg ctttgacttc
      121 gtcatcgctc aaaccggcat tgccgccttt ggccggcatc gcgttaaagc cttcaagggc
      181 gtgtttgtgc aaggtttctt tgcctttttt gatacgcggt gcccaatcgt cttttttgcc
      241 tatgccggga ataccgggaa tcgaaccgcc gtggcacacc tgacaggttg cttcgaagac
      301 ttttttaccg tcaacgccga ccgcaggggc tgccgcaccc ttgtcttctg ccttcgcttc
      361 tgccggagct gcactatcgg caggagcaga agctgttcct gaagcggcat tgtcggcagg
      421 cgcagcctca tcaggattcg ggaaagaacc gccgcttttg ttcgccatgt aagtaatcgc
      481 ccgtttgagt tcctgatcgg tcaggtctgc cgcaccgcct ttggcaggca tggcgttaaa
      541 gccgttcagc gcgtgttgga acaaggtatc gaagccttgc gcgatacgcg gtgcccaatc
      601 gccgttgtgt tccagtttcg gagcgttcgg cacattgctg tccgccgcgt ggcattggat
      661 acagattttg ccgaaaatct gttcgccttg gcgttcgccg acggggatgc cgtcgcccat
      721 cgtcaattgt ccgacaggct ggatacgggt ctgcgttgct gcttccgtag tggcatcgac
      781 atcgccgaac gagccgctgc ccgccagctt aatcaggaaa taaaggactg caataacaat
      841 aacgataccg ctcacaaggg taaacagtgc agagccttgg gctttgttgt cgcggagttg
      901 tttcatttgg taggcctcgc cgtcaggtta ggttgtgctg taaattatag tttggtgtgt
      961 taaacgcagt taacaatatt ttgctggatt atactgaatt cacagggtct ttccaatcgc
     1021 tatcattgaa aatatgaaaa aatttgccaa cggtatctgt ataaaacaaa taatcctttg
     1081 aaaataatgg tttatcctca agaaaactct ccttatgccg ccatacgccg cctgccggcg
     1141 caagataacc tttgccaatt tgcagaattt acgttaacct tacgttttac gcacccatag
     1201 ctcagttgga agagtgtcag tttccgaagc tggaggtcac aggttcgatc cctgttgggt
     1261 gcgccaatta taaagagacc gtctgaaaga taaatatttt tcagacggtc ttttgactta
     1321 cttcaaactc ttatttcaag acttccgcaa atgcgcgggc aacatagtcg gtattcgacg
     1381 tattcagtcc ggcgacgcac atcctgccgg aatccagcag gtaaacggca aattcgtcgc
     1441 gcagcctgcg gacttgttcc acgctcaatc ctgtgtagcc gaacatgccg cgctgtttga
     1501 tgaaataagt gaaatcgcga tcggggattt gcgcagttaa gacatcataa agtttctgcc
     1561 gcatcgcacg gatgcggtcg cgcatcatat aaacctcgtt ttgccacaag gcgtaaagtt
     1621 cggggctgtt catcacgtcg gcggcgatat acgcgccgtg cgcgggcggg ctggagtaga
     1681 tgcggcggac ggtgaatttg agctgtccga acaccaaatc cgcttcttcc ttattcgggc
     1741 aaaccacgct caggccgccg acgcgctcgc cgtagagcga caggtttttt gagaaggaat
     1801 tgctgacgaa caagggcaat tccatttcca ccgctttgcg gacggcgtag gcatcgctgt
     1861 ccaaatcgcc gccgaagcct tggtaggcga tgtccataaa cggaatcagt ttgcgcgttt
     1921 tgatgatgtg caacacttcg tcccattgtt gttccgacat atccacgccg gtcgggttgt
     1981 ggcagcaggg atggaggatc aggacgctgt tttcgggcag ggtgttgaaa aacgcggtca
     2041 tttcgtcgaa tttcacgccg acggcagcag ggtcgtaata tgggtaagtg ccgacctcga
     2101 aacctgcgct ttcaaaaatg ccgcgatggt tgtcccaagt cgggtcgctg acgtaggcgc
     2161 gcgcttcggg aaaccagcgg tgcagaaagt ccgccccgac tttgagcgcg cctgagccgc
     2221 ccaaggtttg tacggtaaca atacgtcctt gcgcgagcgc ggggttgtct ttgccgaaca
     2281 acaaatgctg caccgcgctg cggtaagtgt ctaagccttc catcggcagg tagggcgacg
     2341 gcacaggagt ggcggcgcgg gcggtttcgg cgcggctcac ggattccaac acgggcattt
     2401 tgccttcgtc gtcgaaataa atgcctatgc tcaaattgac tttttcgggg cgcgggtcgt
     2461 ttttgaaggt ttcgaccaaa ctcaaaatcg gatcgccggg atagtattcg atgtgtcggt
     2521 acatagtcct tacctcttgc tttttccaag gattttcttt ttcaacagca caccgctttc
     2581 aatatggtgc gtaaacggaa attgatcgaa cagggcggca cgttcaaccg tatgggtttc
     2641 cgccaaggtg tccaaattgg tacgcagcgt ttcgggattg caggaaatgt agatgatgtt
     2701 gtcaaactgc gacaccagct tcaaagtttc ctcatcgata ccggcacgcg gcggatcgac
     2761 gaaaatagtg gaaaatgcgt aatccgtcaa agcaataccg ccatccttaa ggcgtttaaa
     2821 ctcacgtttt ccggtatagg cttcggtaaa ttcttcagca gacagacggg cgattttgat
     2881 gttgccgatg cggttggctt cgatattcca ttgcgccgcg ctgacggagg ttttggagat
     2941 ttcggttgcc aaaacacgtt caaacttttc agataaagga agcgtaaagt tgccgtttcc
     3001 gcaatacagt tcgagcaaat ccccatccaa tccttctgct gcaccgcagg cccattcaag
     3061 cattttctga cacacggcgg cattcggttg ggtaaaactg ccttcaattt gccgataacg
     3121 gaaatcccgg ttgccgacct tcaaagtttc cgttacatag tcctgtttta agactatttt
     3181 ctgtcccctg ctccgcccga tgacggaaat atccaactgt tgctgtaacg cttgcgccgc
     3241 ctgcatccac tcattatcaa gccttttgtg gtaaatcatg gtaaccagca tttccccgct
     3301 gagcgtagac aaaaattcga cggcatacca acggtttctc agttcggcag attgggcggc
     3361 aacggcaatc aattctggca tcaggcggtt gacggcttcg gaagccgctt caaaacggtc
     3421 gcaacggatc aggcttgccc cgccagcctt ttgtcctttt tcaaacatgg cataaaacat
     3481 ttcaccgcct tcgtgccaaa tacggaactc ggcacgcata cggtaatgtt tgtccggaga
     3541 ttcgtacact tcccactcag gaacatccaa acctgcaaaa agggttttaa ggtggtcttt
     3601 tttatcttga agctgctgcg tgtagtcgct catatcgcta tcctgaaaaa tcagaccgga
     3661 ttataagccg attcactgca aaacacgcaa cccctttacc ggcatataac ccgttttcct
     3721 ccaactttta tgctaaaatc cgccgtctcc aacataccgc cgacagaagg aaaatatggc
     3781 aggcaacact ttcggacaac tcttcaccgt gaccacgttc ggcgaaagcc acggcgcagg
     3841 tttgggctgc atcatcgacg gctgcccgcc cggcttggaa ttaagcgaag cggatatcca
     3901 atttgacctc gaccgacgca aacccggcac cagccgccac gttacccaac gccgcgaaac
     3961 cgaccaagta gaaatcctct ctggcgtatt caaaggcaaa accaccggca cgcccatcgc
     4021 cctcttaatc cgcaataccg accagcgcag caaagactac ggcaacatcg cccaaagttt
     4081 ccgccccggc catgccgact atacctattg gcacaaatac ggcacgcgcg actaccgcgg
     4141 cggcggcagg agctccgccc gtgaaaccgc cgcccgcgtt gctgccggag ccgttgccaa
     4201 aaaatggttg aaagaaaaat tcggcacgga aatcaccgcc tacgttaccc aagtcggcga
     4261 aaaagaaatc cggtttgaag gctgcgaaca catttcccaa aatccttttt ttgccgccaa
     4321 ccatagccaa attgccgagc tggaaaacta tatggacagc gtgcgcaaat cgttggattc
     4381 cgtcggcgcg aagctgcata ttgaagccgc caatgtcccc gtcggcttgg gcgaacccgt
     4441 tttcgaccgc ctcgatgccg aaatcgccta cgcgatgatg ggcatcaacg ccgtaaaagg
     4501 cgtggaaatc ggcgcaggtt ttgacagcgt aacgcaacgc ggcagcgaac acggcgacga
     4561 actgaccccg caaggcttcc tgtccaacca ttcgggcggc atcctcggcg gcatcagtac
     4621 cgggcaagac atctgcgtca atatcgccat caaacccacc agctccatcg ccaccccgcg
     4681 ccgcagtatc gacatcaacg gcaaccccat cgaactcgcc acgcacggca ggcacgaccc
     4741 ctgcgtcggc ttgcgcgccg cgccgattgc cgaagctatg ctcgccttgg ttttaatcga
     4801 ccacgccctg cgccatcgtg cgcaaaatgc ggatgttgcc gtggatacgc ccgatatcgc
     4861 ccgctcggac aagtaaaaag atttagccaa aacacgggct ttatactaaa ataccgacca
     4921 ttgccgtgcc gtctgaacgc acgattctta gaggaataca accgaaatga cacaagaaac
     4981 cgctttgggt gcggcactga aatccgccgt ccaaactatg agcaaaaaga aacagaccga
     5041 aatgatcgcc gaccacatct acggcaaata cgatgtgttc aaacgcttca aaccgttggc
     5101 actcggcatc gatcaggatt tgattgccgc tttgccgcaa tacgatgccg cactgattgc
     5161 acgcgtcctc gccaaccact gccgccgtcc gcgctatctg aaagccttgg cgcgcggcgg
     5221 caaacgtttc gatttgaaca accgtttcaa aggcgaagtt acccccgaag aacaggcgat
     5281 cgcgcaaaac catcctttcg tgcagcaggc tttgcaacag cagtccgccc aagctgccgc
     5341 cgaaacgccg tctgttgaag ccgaagcagc cgaatcttcc gcagcagaat aaatccccaa
     5401 atgaaatgcc gtctgaaaac cgatttggtt tcagacggca tttttccgta tgcggcaatc
     5461 acggttcaaa tatcgagttc cgccgtatcg ccttcgcgct ccatccaggc acggcgggcg
     5521 gcggcttcgc ctttgcccat cagtttgacg aagatgtcgc gcgtctcgtc atccgcgcct
     5581 tcggggattt gtacctgcaa caggcggcgg gtgtcggggt gcatggtagt atctttgagc
     5641 tggtctgggt tcatctcacc caaacctttg aaacggctga tggaataagc agtttcttta
     5701 acgccttctt tttgcagccg ctctaaaatg ccgtcaagtt cgttttggtc gagggcgtag
     5761 aatttgcggg cgggtttgct tttaccttgt gcgttgacat ctacgcggaa cagcggcggc
     5821 tgggcgacgt agatgtgtcc gtcagcgacc agtttcggga agtggcggta gaacagggtc
     5881 agcagcaaaa cttgaatatg cgagccgtcc acgtcggcat cggacaggat ggcgattttg
     5941 ccgtaacgca ggccgcttaa atcgggatgg tcgttgatgc cgtgcggatc gacgccgatg
     6001 gcaactgaaa tgtcgtggat ttcggcgttg ccgaagagct ggtcggggtg gacttcaaag
     6061 ctgttgagca ctttgccgcg caggggcagg atggcttggg tggctttgtc gcgggcgagt
     6121 ttggcggagc cgccggcgga atcgccttcg acgaggaaga gttcgttttc gcggatgtct
     6181 tcgctttcgc agtcggtcag cttaccgggc aggacggcga cgccgctgcc ttttttcttt
     6241 tcaatctttt taaccgaacg catccgcgcc tgtgcctgac ggatggcgag ttcggcgatt
     6301 tttttgccga agtccacgtt ttggttcagc cacaattcca aagggtcgcc cgatacggta
     6361 gcaacgagtt tcagcgcgtc gcggttggtc agtttgtctt tggtctgacc ttggaactgc
     6421 gggtcgagga cacgggcaga gaggacaaag gcggttttgc cgaacacgtc gtcgctttgc
     6481 actttcacgc cgcgtggtaa aaggttgtgc agattgatga agttgctgac ggcgttgaac
     6541 acggcttgtt tcaagcccgc ttcgtgcgtg ccgcccagcg gggtggggat gaggttgacg
     6601 tagctttcgt tggcgcacga gccttcttcc agccaggtca gggcaaacgc ggcgccttcg
     6661 ccgatgctga aatcgccgtt gtggccgtct gaaatgtagt tttcgcaaga gaagataggc
     6721 acggcttcct gcgcgtcggc aatcaggtcg gtcagatagc ttttcagacc gtcggggtaa
     6781 tgccaggttt gggtgtgcgc ttcgtcttcg cctttgaccg gacgggtcag ggaaacgcgc
     6841 acgcccggca gcagcacggc tttggcgcgc agcaggcgtt cgagttcggg gatgctgtaa
     6901 ttcgggcttt caaaatattt gccgtccggc cagacgcgca cttcggtgcc gctgtctttg
     6961 acggcgcatt tgcccacttg tgccaacggt tcgaccacgt cgccgccggc aaacacgatg
     7021 cggtggattt tgccttcgcg tttgaccgtt acctcaaggc gggtggaaag ggcgttggtc
     7081 acggatacgc ccacgccgtg caggccgcct gaaaaggcat acgcgctgcc gccgtctttt
     7141 ttgttgaact tgccgcccgc gtgcagacgg gtgaacacga gttcgactac gggtacgcct
     7201 tcttcagggt gcagaccgac gggaataccg cggccgttgt cgcgtacgga aagcgaaccg
     7261 tcttcatgaa tttgcacgtc gattgccgta gcgaaaccgc ccaacgcctc gtccgccgcg
     7321 ttgtcgatga cttcttggca gatatgggtc gggctgtcgg tgcgggtgta cataccggga
     7381 cgttctttga ccggttccaa acctttgagg acggtgatgc tggattcgct gtattggttg
     7441 tttttagcca taggaataat ctgaaagtaa gaaaaacaac gctttcagac ggcctgaaag
     7501 cgttgcgttc cgttgtttta ccggttgccg gaaagttggc gggcggcaaa gtcttcataa
     7561 ctttccatac cgcgcaggaa gcgggaagaa agttctttca atgcccccaa ataaacgtcg
     7621 cgtttgaaat caatcacttc gtcaacgggc gcccaatatt ggtgccaacg ccagccgtca
     7681 aattcggggt ggcgggtggc gcgcaggttg acatcgcaat cccggccggt caggcgcagg
     7741 agataccaaa tctgcttctg cccgcgataa gagccgcgcc attcgcggcg tacccagttg
     7801 ttcggcacgt cgtaacgcag ccagtcgcgc gtccgcccga cgattttgac gtgttgcgac
     7861 agaagtccga cttcttcgta aagctcgcgg tacatcgcgg tttcggggct ttcgcccggc
     7921 ttgatgccgc cttgcggaaa ctgccaagaa tgttcgcgca cgcgcttacc ccaaaagact
     7981 tcgtcgcggt tgttggttaa gatgataccg acattggggc gatagccttc cctgtccaac
     8041 acggtgtcgc cctccgttaa attcaatctt gggattttcc cacaaatcga acggttttga
     8101 caaatcagac ggtatggttc aagcatgtcg aaacacagac ggactcggga aaatatctta
     8161 aatttggttt acaataatgt gtttcaaatt attcgggaat cagaccatgt tagacatcca
     8221 attgctccgc agcaacaccg ccgccgttgc cgaacggctt gcacggcgcg gttatgactt
     8281 tgataccgca cgttttgacg cactggaaga acgacgcaag tccgttcagg tgaaaaccga
     8341 agaattacaa gcctcgcgta acagcatttc caagcaaatc ggcgcactga aaggtcaggg
     8401 caaacacgaa gaggcacagg cggccatgga tcaagtcgcc caaatcaaaa ccgatttgga
     8461 acaggcagcc gccgatttgg aagccgtgca aaaggaactg gacgcatggc tgttgagcat
     8521 tccgaacctg ccgcatgagg acgtgccttt cggtaaggat gaaaccgaaa acgtcgaagt
     8581 ccgcaaagtc ggcaccccgc gcgaatttga ctttgaaatc aaagaccatg tcgatttggg
     8641 cgaacctttg ggtttggatt tcgaaggtgg cgcgaaactt tccggcgcac gatttaccgt
     8701 gatgcgcgga caaatcgccc gtctgcaccg cgccttggca cagttcatgc tggatacgca
     8761 cacgctgcaa cacggctaca ccgagcatta cacgccttat atcgttgacg atacgacgct
     8821 gcaaggtacg ggccaactac caaaatttgc ggaagatctg ttccacgtta cccgtggcgg
     8881 cgacgaaacc aaaaccaccc aatacctgat tccgacagcc gaagttaccc tgaccaatac
     8941 cgttgccgac agcattatcc cgtccgaaca actgccgctg aagctgaccg cgcattcgcc
     9001 ctgtttccgc agcgaggcgg gttcgtacgg caaagacacg cgcggtctga ttcgccagca
     9061 ccagttcgac aaagtagaaa tggtacaaat cgttcatccc gaaaaatcat acgaagcact
     9121 ggaagaaatg gtcggccatg ccgaaaacat cctgaaggct ttggaactgc cctaccgcgt
     9181 gattaccctg tgtaccggcg acatgggctt cggcgcaacc aaaacgtatg acttggaagt
     9241 ttgggttccc gcgcaaaata cctaccgcga aatctcaagc tgctccaact gcgaagattt
     9301 ccaagcccgc cgcatgaagg cgcgtttcaa agacgaaaac ggcaaaaacc gcttggtaca
     9361 tactttgaac ggctccggct tggctgtcgg cagaacgctg gtcgccgtat tggaaaacca
     9421 tcaaaacgcc gacggcagca tcaacatccc tgccgcactg caaccgtata tgggcggcgt
     9481 tgccaagttg gaagtgaaat aagtttgcaa gctgcctgaa cgtcaaatgc cgtctgaaac
     9541 ctggttcaga cggcatttcc tttaaacttt taaaacacgt cagccgtcgg cacgaaccgc
     9601 attgccgcaa tcgccggtct gtccgacctc gcggatattg gacagcgtaa cttccgaaat
     9661 attacccaac gcctcttccg tcaaaaacgc ctgatgaccg gtaaacagca cgttatggca
     9721 ggacgacaac cggcggaaca cgtcgtcggt aatcacatcg ttggatttgt cttcaaaaaa
     9781 cagctcgcgc tcgttctcgt acacatccat gcccaatgcg ccgattttcc ggcgtttcaa
     9841 cgcctcaatc gcggcggcac tgtcaatcag cccgccccgg ctggtgttga taatcatcac
     9901 gccgtctttc attttgtcga acgccgcttc gttcagcata tagtggtttt ccggcgtggc
     9961 ggggcaatgc agcgtgatga tgtccgactg ggcatacagc tcgtccaaat ccacatacct
    10021 gccgccgatt ttttccgctt cggggttgca aaacggatcg tgagccagca ggttcatgcc
    10081 gaaacccttt aaaatccgca tggttgcaat accgattttc cccgtgccga taacgcccgc
    10141 cgttttgccg tacatattga aaccggtcag accttccagc gaaaaattcg catcgcgggt
    10201 acgctgatag gctttgtgga tacggcggtt caaagtcagc atcagaccga ccgtatgttc
    10261 cgcaaccgat tcgggcgaat aggcaggcac gcgcacgact ttcaagccca actcttcagc
    10321 cgcctttaaa tccacattat tgaagccggc acaacgcaac gccacagttt tcacgccaat
    10381 ttgcgccaat ttttccaaca cgggccggct gccgtcgtcg tttacaaaaa tacaaaccgc
    10441 ttccgcgcct tccgccattt tcgccgtttt cgcatccagc ataaaatcaa aaaactccag
    10501 ctcgaagccg aaatgccggt tggcgcgggt aaaatgttcg cggtcatagc ttttcgtacc
    10561 gtaaatcgca atcttcatca atatgtccag ttgtcgccta tggttgagaa acggcataat
    10621 acactgaatt caaacaaatc agtagaatat ggcagattaa aattgattgc atgcacggca
    10681 tttccatttc aaaacacaaa actcaatcgc ccattgccgc caaaagctcc gcctgatgtt
    10741 cggcaatcag ggcatcggtg atttctgcca aatcgccgtc catcacaaaa tccagcttgt
    10801 gcagggtgag gttgatgcgg tggtctgtta ccctgccttg gggatagttg taggtgcgga
    10861 tgcgttcgct gcggtcgccg ctgccgatga gggatttgcg ttcggcggct tctttggctt
    10921 gggcttcgcg tttttgcgcg tcgttcaggc gggcggcgag gactttcatc gcctgcgctt
    10981 tgttggcgtg ttggctgcgt ccgtcttggc attcgaccac catgcctgtg ggcaggtggg
    11041 tgatgcggac ggcggaatcg gttttattga tgtgctgccc gcccgcgccg gatgcgcgaa
    11101 aggtgtcgat gcgaaggtcg gctgggttca gttcgatgtc ttccagttcg tccgcttcgg
    11161 gcatgacggc aacggtgcag gcggaggtgt ggatgcggcc ttggctttcg gtggcgggga
    11221 cgcgctgcac gcggtgtccg cccgattcaa atttcaaacg gctgtacgcc ccgagtccga
    11281 caatacgggc gatgacttct ttatagccgc ccaattcgct ttcgttggcg gacacgattt
    11341 caacctgcca acggttgcgc tcggcgtagc ggctgtacat acgcagcaaa tcgccggcaa
    11401 acagcgcggc ttcgtcgccg cccgttccgg cgcgtatttc gatgaagatg tttttgtcgt
    11461 cgtcggcatc tttgggcagc agcagttttt gcagttcggt atcgagttcg ccgattttgg
    11521 ctttggccgc ttcgatttct tcggcggcaa aatctttcat ttcggggtcg gacagcattt
    11581 cttcggcatc tgccaagtcg ctttgggcaa gccgatagtt ttggaatact tcgacgacgg
    11641 gggtcagttc ggcgtgttcg cgcgtgagct tgcggtagtt gtccatatcg gatgtggctt
    11701 cgggttgccc taaaaggtgg gtaacttctt ccaaccggtc gccgagttgt tgcagttttt
    11761 ctaagataga cggcttcata attcttccat aacaaacgcc gcctgaattt cagacggcat
    11821 caaaccgcgg tcattataat atattttctg aatattcaaa aagataatct agggacggag
    11881 caggctgtcg gcattgcgtt ccgttatcgc ggcgatttcc gccgcgccgg taccgcgtat
    11941 ctgcgcggca atttcggcaa tccgaagaat gttggcaggc gtattaatct cttttttcag
    12001 cataaacggg ctgtcggttt ccaatacgaa atccccgtcg ttcaaggctt taagcgtatc
    12061 gcgcacttta cgcgcgttcg gattgagcaa caacgaaccg atgccgattt tgaaacccag
    12121 tttcgtcaac acgcgcgctt cttccgcgct gcctgagaag gcgtgaacga tgccgccttg
    12181 ggcaaagcct gtctgtttga cggcggcggc gatgtcggcg gtggctttga gattatggat
    12241 aatcacgcgg cggcgcaggg tttgcgcgat ggcaagctgg cggacgaaaa cttggatttg
    12301 ccgttcgcgc tgcggcggcg tttgggtttt gtcgtaaaaa tccaagccga tttcgccgac
    12361 ccatgcctgc ggataacgtg ccaacatcgt ttccaggcgg acgcaatcac gctcggcaat
    12421 gccgtctgaa aaccaaggat ggatgccgag cgcaatacgg atttgaccgt gttcggacgg
    12481 catttccgac agttccgcca cgctttgcca atcctgcggg cgcgtcgcgg gaacgataaa
    12541 ccgccacaca cccgcctgtc gcgcttcggc aagcacctgc gccagattgc cgcgcaaaac
    12601 agaatcggca agatggcaat gtgtgtcggt cagcctcatc ttcacaccag ccccaaccgc
    12661 tgcaactcgt gtttcacctc gtccgcgccc catccttcag aaacggcaag cgtcatcaat
    12721 gcggttgcgg tttccaaatt gcatttgccg ccgttgatga tgcctgagtt gcggaacgcg
    12781 ttgccttgcg cgtaaacggc agcggttctg ccttgtcgga cttggctgat gttgagcagc
    12841 agtttgcctt gctgcgtaaa gtcttgcacg gcgcggatga aaccttcgtc tgcgggcgtg
    12901 ttgccgtgtc cgtagctttg caggataagg gcttgggcgg gaagctgtcc gagtccgtcg
    12961 gcaagttctt ggacggcaaa gccggggata agcgtgcgga cggcgatttt tgcctgcggg
    13021 tcgggataac ggattttgag gccgtctgaa acatctgccg cgtcctgatt cgggatgcgg
    13081 acattgtgcc agccctgggt ttcgtcccac tccgccagtg cgccgaaatg cggattgtcg
    13141 aaaccggcgg cggtttcggt gctgactttg ctgctgccga cggcgggata aagtttgccg
    13201 tcaaaggcaa taagcgtctg cttcaatccg agtgggaatg cggcaacggc agtcgccaga
    13261 ttgcgcggcg cgtcgctgcc cacggcgttg tacggccatt gggaaccggt caggacgatt
    13321 ggcttgtcca aaccctgcag cgcgagggcg aacagattgg cggtataggc gagcgtgtcc
    13381 gtaccgtgca gcaccaaaac gccgtcgtac tgcggcagcg cggcggcaat gatgtccagc
    13441 caagcgcacc agttttgcag ttggacggcg gaagaatcaa tcagcgtccc acaaacgtgc
    13501 caatcgaaat ccatgccgtt tgaaaacggc gcgagtgcct gcccgaccag cgcggtatcg
    13561 gggcgcaggc cttcgctgct ttgcgtcatg ccgatggtgc cgccggtata aaggacaaaa
    13621 attttctgtt tcatggctca tcctgaaaat gcaaagaccg cctgaaacgc gaaatgcgtc
    13681 cggacggtcg aaaatctgat ttactgctgc ttctgtgccg cttttttcgc tgccttttcc
    13741 gccttgcgtt tggcgcgttt ttcgctcaat tgtgcgcggt aaagctgata atgtcggcgt
    13801 ttgcgccacc agaaccacgc cagcgccgcc gccagcacgc ccaatgcgat gaagatgccg
    13861 gattgcaggc tgtgcatttt cgccatcagc caatcgatgt tgtgcgcgcc gtactcgccc
    13921 aagtaaatcc aaacgggcac ggaaatcagc gcggcaagcc cgtccataat cagaaagcgc
    13981 agatacgata ctttgcggct gatgccggcg gtaacgaaaa cggcagtccg caaacccggc
    14041 aggaaacgag cgacaaacag aacccagttg ccgtatttgt cgaatttttc ctgaacctgt
    14101 gcgtaacgtt tcggcgtcat gatgcgcgca atcggtttga acttgaggat tttctgcccc
    14161 cagatgcgtc cggcggcgaa catgatgccg tccccgacca atacgccgag cataccgact
    14221 gcaaacataa tatgcggatt ggtataaccc atacccgaaa tcacgccgcc tgttaccaag
    14281 gtcaaatcct cgggaatcgg cacgccgaaa ccgcagatga ccaaaacgaa aaacacggcc
    14341 gcatagccgt attcgacaaa aaaggcttcc aaaagggcaa acatagcgga tattccattg
    14401 tcggagataa aaagtcagaa caagccgaaa cattttctgc gcgaaacggc attctatcaa
    14461 agattatgcc gtctgaaagc ggcaaggggc agattcgcgc cctctgccgg acgttcagac
    14521 ggcatttaaa caggaaaacc gccgctgttg aaacggtttt gcctgatttt gcctaaaggc
    14581 tgccgatggc ggaagcaata cgttccgcac cgtcgcgcgc ccaatccgcc tgccgcgcct
    14641 ccaccatcac acgcacgacg ggttcggttc ccgaagcgcg caacacgaca cgccctttgc
    14701 cttcgagttc tttttccact tccgccaaca cgtcttttga ggcttcctgc catttctgac
    14761 ctttttggat gcgcacgtta atcatcgttt gcggatacgg ctgccaatcg gcgcaaacgg
    14821 tggcgaggtc ttggttcagc gtttgcagtg ccgccaaaac ttgcagcgcg gaaataatgc
    14881 cgtcgccggt gttgtgtttg tccatgcaca aaatatggcc gctggcttcg ccgccgatga
    14941 gccagctgcg ttggttcagc tgtgccaaca catagcggtc gccgactttg gcgcggcaga
    15001 aatccacgcc ctgctctttc agggcgattt ccatcgccat attggtcatg accgtgccga
    15061 ccacgccgcc gatgttgata ccttctcggg cgcgggcttt agcaatgacg taaatcaggc
    15121 tgtcgccgtc gtaaacctta ccgtttttat cgaccatcat caggcggtct ccgtcgccgt
    15181 ctaaggcgat gccgtagtcg gcttcatgct gtaaaacggc tgcctggagt gtcttggtat
    15241 aagtcgcacc gcatttttca ttgatattgt agccgttggg ttcgtcgccg atgctgacga
    15301 cttgtgcgcc cagttcgtga aacaccttgg gtgcgacacc gtaccccgcg ccgttggcag
    15361 tatcgataac caacttcaaa ccccgaaggt cggaatggct gggaaaggtg gatttgcaaa
    15421 attcgatata gcggtcgtcc gcaccgctga tgcggcgtgc gcgaccgagg cgggcggacg
    15481 ggagggtttt catttcgccg tcgattttgg cttcgatttc caactcgact tcatcggaaa
    15541 gtttcacgcc gccttcggcg aagaatttga tgccgttgtc ggaataggca ttgtgcgacg
    15601 cggaaatcat cacgccggcg gacaggcgca acgcgcgggt cagataagcc acgccgggcg
    15661 tgggcagcgg tccggtctgt accacattca cacccgccgc cgtaaaacct gccaccaaag
    15721 cggcttccaa catataaccg gaaatacgcg tatccttgcc gattaaaacg gtcggtttct
    15781 ggtcggtgtc gtgctgaacc aaaacctgac ccgccgcata gccgagtttc aatacaaaat
    15841 cgggcgtaat cggaaactgc ccgacttcgc cgcgcacgcc gtccgtgccg aaatattttt
    15901 ttgccatgtg ttgctccgag aatgtgaacc gttgtccgag attatacagt ctgtttgtac
    15961 cttgctgtct gcaccgttga tgccgtctga agccgctccg cccttttcag acggcatgaa
    16021 gtatgtgaac cgctgtttac agattgatgc ccaacgcttc ccacgccttc aaagcatccg
    16081 ccgtcgcctt cacatcgtgc acgcgcacga ttttcgcgcc gcgtgccaca gccgccaaag
    16141 ccgccgccac gctgccgtgt ccgcgcgccg ccgcgtcggt ttcgcccgtc agttcgccta
    16201 tcatgctttt gcgcgacacg ccgatcagca gcggatagcc cgtttccgcc atcaattcgg
    16261 gcagatgccg catcagtgtg atattgtgtt gcaaggtttt gccgaagcag aaaccggggt
    16321 cgagtgtaat gcgttgcggg gcgatgcctg ccgcgatgca ttccgccgcc cgcgctttca
    16381 aataccgcgc cacttcaccg acaacatcct gatatttcgg attaatctgc atattcttag
    16441 gcaagccctg catatgcatc aggcaaatgc ccgtatccgc ctgacacgcc agcaattcga
    16501 gcgcgccttc gtcggtcaac gccgccacat cgttaataat atcgatgccg ccgagtgcca
    16561 acgctttttc cataaccacc gtgtggcgcg tgtccaaact gacgggaacg ccccaccctg
    16621 ccacttccgc caatacagac tcaacccgcg cccattcttc ttcaggcgaa acataatccg
    16681 cacccggccg cgtcgattcg ccgccgatgt cgagaatgtc cgcgccctct ttcaaaagcc
    16741 gttcggcatg cgccaaagct gtttgggcgt tttgcgaata cgcgccgccg tcggacaaag
    16801 aatcgggcgt gagattcacg atacccatga ttttcggttt gtccaaaccg atttcaaacc
    16861 gtcctgcctg ccaaacgcat ccgaccatat ccgtgcctcc ggaataaaaa gcaaattata
    16921 taccgtctga acaaaacttg tccgtttcag acggcatcag tatttcaaaa aagtatcttg
    16981 cagaatccgc cgccgtatga aacatacact gcccctgcaa acgcggcaac gcccacgata
    17041 cctgatccgg cggtatgcct gcttatgggc attacccctt tatacaagat aatccgccca
    17101 cacaaaatag gatttctaca agccgtgtta tactgtggcg tgttttacag acttttcagg
    17161 ctatggattt attatcggtc ttccacaaat accgtctgaa atatgcggta gccgtgctga
    17221 cgatactgct tttggcggca atcgggctgc acgcttccgt atatcgcatc ttcacacctg
    17281 aaaacatccg aagccgcctc caacaaagca ttgcccatac gcaccggaaa atctcgtttg
    17341 atgcggatat acagcgcagg cttctgcccc ggccgaccgt catcctgaaa aacctgacca
    17401 ttaccgaacc cggcggcgac cggactgccg tttccgtcca agaaaccaaa atcggattga
    17461 gctggaaaaa cctgtggtcg gatcagatac agattgaaaa atgggtggtt tcgagtgcgg
    17521 aacttgccct gacgcgcgac gggaaaggtg tttggaacat ccaagacctg atcgacagcc
    17581 aaaaacgcca agcctcagtc aaccgcatta tcgtcgaaaa cagcaccgtc cgcctcaatt
    17641 tcctgcagga acagcttatc ctgaaggaaa tcaacctcaa cctgcaatcc cccgattcgt
    17701 cggggcagcc gtttgaaagt tcgggcatac tggtttgggg aaagctgtcc gtcccgtgga
    17761 aaagcagggg gctgttcctt tcagacggca tcggcacgcc caaaatctca ccgttccatt
    17821 ttgaagcttc cacttcgctg gacggacacg gcattaccat ttccaccacc ggcagccctt
    17881 ctgtccgctt caacgccggc ggagcggatg ccgccggcct cggcctgcgt gcagacactt
    17941 ccttccgcaa cctccacctg accgcccaaa tccctacgct ggcactcagg aacaacagca
    18001 ttaaaattga aaccgtcaac ggcgcattta ccgccggcgg cgaatatgcc caatgggacg
    18061 gttcgttcaa actcgacaaa gccaacctgc actccggcat cgccaacatc ggcaacgccg
    18121 aaatctccgg cagcttcaaa acaccgcgcc accagaccaa cttctccctc aattcgccgc
    18181 tcgtatggac ggaaaacaaa gggctggacg cgccgcgcct gtatgtatcg acccttcagg
    18241 ataccgtcaa ccgcctgccg caaccccgtt tcatcagccg gctcgacggt tcgctgtccg
    18301 taccgaatct gcaaaattgg aatgccgaat taaacggcac attcgaccgc caaaccgttg
    18361 ccgcgaaatt cagatacaca catgaagacg caccgcatct ggaagccgcc gtcgcactgc
    18421 aaaaattgaa cctgaccccc tatcttgacg acgtgcggca acaaaacggc aaaatatttc
    18481 ccgacaccct cgccaagctg tccggcgaca tcgaggcgca cctgaaaatc ggaaaagtcc
    18541 aacttcccgg cctgcaactg gacgatatgg aaacctacct ccacgccgac aaaggccata
    18601 tcgcgctcag ccgtttcaag tcagggcttt acggcggcca taccgaaggc ggcatcagca
    18661 tcgccaacac ccgtcccgcc acttaccgcc tgcaacagaa tgcaagcaac atccaaatcc
    18721 aaccgctgct gcaagacctg ttcggcttcc acagcttcag cggcaacggc gacgcggtca
    18781 tcgacctgac cgcgggcggc gaaacccgaa aagagcttat ccgctcgctt caaggcagcc
    18841 tgtcgctgaa tatttccaat ggcgcatggc acggtatcga catggacaat atcctgaaaa
    18901 acggcatttc gggcaaaact gccgacaatg ccgcacccag cacacccttc caccgattca
    18961 cgctcaacag cgaaatttca gacggcatca gccgccacat cgataccgaa ctcttctccg
    19021 acagcctcta tgttaccagc aacggctata ccaatctgga tacgcaggaa ttgtctgaag
    19081 atgtccttat ccgcaacgcc gtccatccga aaaacaaacc gattcccctg aaaatcaccg
    19141 gtacggtgga caagccgtcc attaccgtcg attacggcag gctgaccggc ggcatcaatt
    19201 cgcgcaaaga gaaacagaaa atcctcgaag acaccctgct ggaacaatgg cagtggctca
    19261 aacctaaaga accgtaaaca tcctgcgtac aaaaatgccg tctgaaacac ccccgcgctt
    19321 cagacggcat gaccgtaaaa cctacaaccc caattcctcc caaatcccat caatcttagc
    19381 cgtaaccgca gggtcttttt tgatgacgcg tccccattcg cggtcggttt cgcccggcca
    19441 tttgttggtc gcatccaaac ccattttgcc gccgagtccg ctgacggggc tggcgaagtc
    19501 gagataatcg atgggcgtgt tttctaccaa aacagtgtcg cgcacggggt ccatgcgcgt
    19561 ggtgaccgcc cagatgactt ctttccagtc gcgtacgttc acatcgtcat ccaccacgat
    19621 gatgaatttg gtgtacataa actggcgcag gaacgaccag cagcccatca tcacgcgctt
    19681 ggcgtgtccg gcgtactgtt ttttcatgct caccaccgcc atgcggtagg agcagccttc
    19741 gggcggcagg taaaaatcgg tgatttcggg gaactgcttt tgcaagagcg gtacgaacac
    19801 ttcgttcaat gccacgccca aaacagcggg ttcatcggga ggtttgcctg tgtaggtcga
    19861 atggtaaatc gggttttcgc gcatggtgat gcgctcgacc gtaaatacag ggaaatgatc
    19921 ctgctcgttg taatagccgg tgtggtcgcc gtatgggcct tccaacgcgg tttcgttcgg
    19981 atgaatcacg ccttccaata caatttcagc acgggcaggc acttgcaaat cgttgccgat
    20041 acatttcacc agctccgtcc gcgaaccgcg cagcagtccg gcaaactggt attcgctcaa
    20101 agtatcggga acaggcgtta ccgctccgag aatagtggca gggtcgcaac cgagcacgac
    20161 ggcgacggga tacggcgtat cgggattgag tttgcggaac tcctgataat ccaacgcgcc
    20221 gccgcgatgc gacagccagc gcataatcag cttgtttttg ccgatgagtt gttggcggta
    20281 aatgccgaga ttttggcgtt ttttgtgcgg cccgcgcgtt acggtcaagc cccatgttac
    20341 cagcggcgca acgtcttccg gccagcaatg ctgaatcgga agtttataca aatcaacgtt
    20401 ttcgccttcc cacacgattt cctgacacgg cgcgtttttc accacgttcg gcgccatgct
    20461 ccaaatgtct ttcagtagcg gcagtttgga aaacgcatct ttgatgcctt tgggcggttc
    20521 gggttctttc aaatacgcca gcgtctgccc gatttcacgc agcttggaca cgctgtccgc
    20581 gcccatgccc atcgccacac gttcgggcgt gccgaacagg tttgccaaca caggataacc
    20641 gtagcgcgta ccgtcgggct taaccgggtt ttcaaacagc aacgccggcc cttcggcgcg
    20701 cagcacgcgg tcggcgattt cggtcatttc caaatgcggg gaaacggggt gcgcgatgcg
    20761 tttgagtttg ccctgctgct cgagcatggc gatgaagtcg cgcaggtctt tgtatttcat
    20821 attcatcctt tttgtccttt tatcctgagc aatccgattc ggataccgcc cctatccttg
    20881 cctgcgcttc ggcatattct atgccgtgat aaaagtcgcg taccagcgga tgttcgctgc
    20941 cttgatggag ttgcaacaaa ggacgttgac catcgggttg ggtaacgaca ttgcaatgca
    21001 aaccgaaggt gtcggtttca taagggggca gccggttgca gatcatcccg aaataaacga
    21061 cattttccgg attgtcgtga aagtggcttt gatagtcgtt aaaactcttt tcgctgacgg
    21121 acacccacac gccgtattcc agcgtttcct catgcccgat aatcggaata agcagcaccg
    21181 cgcggataaa acggtcggtt tggtcgggat agcggatgat gcagaaatcg ccgccgcatt
    21241 cagcttggta agcaatgcgt tcttcttcac tgagttgatt atagggatag ggggcggtaa
    21301 agccgattgc gggcatatct tcatgttttt cgccgcagga agtgcaagtg tacataaggt
    21361 ttcagacttt caaaacgagt ttgcggtaaa gccattcgcc ggcaaacggc atccccatca
    21421 atacataggc aatcacgccg gtataaaccg cccaccaatc atatcgcccc aaccgtgcca
    21481 acaaagcggc aagcgtcccg ttggcaataa agaatacgca ccaaacctgc gttacccggc
    21541 gggtatagcg cacagctttt tcaggcaggt cgggctgttg cagccgcgca agtttttcaa
    21601 tcaccgtctg cccggcaaac aggctgccgc cgaacaccgc caacatcatc agattgacga
    21661 ggacgggata ccaatacatc gaatcatgcc gcccgaacac caatactgcg gcaaaaaaca
    21721 gtgtaataaa caaagccgca taacgctgtt ggggcagttt ggcagtcagg gcgcgcagca
    21781 gccacaaccc gcacatcgcc gccgccagcc agacaaacca gcccgcctcc ctgccgtaat
    21841 atagtggatt aacaaaaacc agtacagcgt tgcctcgcct tgccgtactg tctgcggctt
    21901 cgtcgccttg tcctgatttt tgttaatcca ctataccaca aagcgggata ggcaatgctt
    21961 aatacggtca gaaaaatatg tccgaaaaaa ccgggtttca ttttgaatcc gcacaaatgt
    22021 tttcagacgg catccgataa aaacatgccg tctgaaaaat aattagaaat acccgattag
    22081 cccgcctgaa tcttcaatac cgcctgtacc acgtcgttga cggtgcggac attgcggaaa
    22141 tcttcagcct gcagcttgcg ggctgccaca gccattcacg attgcgatat acccggcggc
    22201 attcccgact gccgttcagc agaaagcgca cccattccaa accgctccaa tatgcctcgg
    22261 gcagcatacc ggcttcagac ggcatatcgt ccgaagcaga taaagtcagg ctgtaacgcg
    22321 tccctttggt cagaaccatc gccaaagcat aagcaaacgg cgcgcgcgtt gccgatacgg
    22381 catatccttc cggcagcgga tcgtccgccg ccaaaaccaa aaccgacccg catccctctt
    22441 ccaacagtga tgccgcttcc gccaatgccg tttccacacc gtcggcacac acggccaatg
    22501 ccgtctgctc gctcatatcc cgcctcaata tcgaccatta ccccgccgtt gcattgtgca
    22561 ccgacaaacc gaacgaagtc ggcgacacgg tttgcgattt caacagttcc aaccacaaat
    22621 cgaaactgcg cgccatttcc ccgtcgtgcg aggcataaac tatagtggat taaattttag
    22681 gggctgtact agattagcag atatgttacc ctcgaaatat gaagataaca cactgcaaat
    22741 taaagaaaaa agtacagaaa gaactgctcc
//
LOCUS       DAWFAD010000013        22450 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWFAD010000013 DAWFAD010000000
VERSION     DAWFAD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.013"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            43..606
                     /locus_tag="WGO49_000394"
     CDS             43..606
                     /locus_tag="WGO49_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0305549.1"
                     /translation="MRHLENNGYANVAGLERILAVKTDNYKEKENLLHEIFSKSRIGD
                     TELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSVHERRQEGNAGSGRKQLLDLV
                     RRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGKSGTRLTDEIADGIHIYTCFS
                     RADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            643..1404
                     /locus_tag="WGO49_000395"
     CDS             643..1404
                     /locus_tag="WGO49_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ0305550.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            1459..1800
                     /gene="fdx"
                     /locus_tag="WGO49_000396"
     CDS             1459..1800
                     /gene="fdx"
                     /locus_tag="WGO49_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ0305551.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(2087..2674)
                     /locus_tag="WGO49_000397"
     CDS             complement(2087..2674)
                     /locus_tag="WGO49_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ0305552.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHYRSRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRTATSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            2887..3084
                     /gene="iscX"
                     /locus_tag="WGO49_000398"
     CDS             2887..3084
                     /gene="iscX"
                     /locus_tag="WGO49_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ0305553.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(3152..3553)
                     /locus_tag="WGO49_000399"
     CDS             complement(3152..3553)
                     /locus_tag="WGO49_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0305554.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTATLREERKQL
                     DQFSRITSAYPDGRPTKRDRRQLDRLKKGDW"
     gene            3764..4723
                     /locus_tag="WGO49_000400"
     CDS             3764..4723
                     /locus_tag="WGO49_000400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ0305555.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLRVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQRLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            4791..6101
                     /gene="tilS"
                     /locus_tag="WGO49_000401"
     CDS             4791..6101
                     /gene="tilS"
                     /locus_tag="WGO49_000401"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ0305556.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKRAGFVPEALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDILALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNTDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNLQGFELHHYAGRLFVFRQEKTDKLRFLKDRQISGNLR
                     EILTGQGFVLKRHPFGLPEHLLEQDGILRTVAASDTLAVGGIHKDVKKILQGKRVLPV
                     LRPIWPLVADSGNRPLALANCCADFQYSVSDGILPVHPDFPILF"
     gene            6175..6960
                     /locus_tag="WGO49_000402"
     CDS             6175..6960
                     /locus_tag="WGO49_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0305557.1"
                     /translation="MKHISSTNNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEGKMPSEEIRRLRAVVPEGRIFSVSDGILKKISSLSCADDILALIDI
                     PLGGTLPDKGDCVVLDGVQDPGNVGTVLRSAAAAGVGTVVLGRGCADAWSPKVLRAGM
                     GAHFLSGIYPQADLEIWLASYKGRVFATALREEKQVVLYGEDLCEPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            6970..7167
                     /locus_tag="WGO49_000403"
     CDS             6970..7167
                     /locus_tag="WGO49_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305558.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLFWMWWKWF"
     gene            7429..7782
                     /locus_tag="WGO49_000404"
     CDS             7429..7782
                     /locus_tag="WGO49_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305559.1"
                     /translation="MKNCKFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            7794..8099
                     /locus_tag="WGO49_000405"
     CDS             7794..8099
                     /locus_tag="WGO49_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305560.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDLVYSSVRPAVKDRAEDAGADDHLDSVRDEHVQNPRENTHKDASNTGAKTH"
     gene            8161..9537
                     /gene="mpl"
                     /locus_tag="WGO49_000406"
     CDS             8161..9537
                     /gene="mpl"
                     /locus_tag="WGO49_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ0305561.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFGVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHYL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     TAGRVKWDLMGRHNRMNALAVIAAARHAGVGIQTACEALGAFKNVKRRMEIKGTANGI
                     TVYDDFAHHPTAIETTIQGLRQRIGGARILAVLEPRSNTMKLGTMKSALPVSLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNAEAGDHILVMSNGGFGGIH
                     GKLLDALR"
     gene            9744..10796
                     /gene="bioB"
                     /locus_tag="WGO49_000407"
     CDS             9744..10796
                     /gene="bioB"
                     /locus_tag="WGO49_000407"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ0305562.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVHQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(10875..11508)
                     /locus_tag="WGO49_000408"
                     /pseudo
     CDS             complement(10875..11508)
                     /locus_tag="WGO49_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            complement(11632..11871)
                     /locus_tag="WGO49_000409"
     CDS             complement(11632..11871)
                     /locus_tag="WGO49_000409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305563.1"
                     /translation="MGGCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            11831..12091
                     /locus_tag="WGO49_000410"
     CDS             11831..12091
                     /locus_tag="WGO49_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0305564.1"
                     /translation="MTKAVRRKNVQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(12154..14013)
                     /gene="ilvD"
                     /locus_tag="WGO49_000411"
     CDS             complement(12154..14013)
                     /gene="ilvD"
                     /locus_tag="WGO49_000411"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ0305565.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHVGRKELFLEAGRMIVEITKRYYEQNDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDITNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPENRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(14186..15955)
                     /gene="cysI"
                     /locus_tag="WGO49_000412"
     CDS             complement(14186..15955)
                     /gene="cysI"
                     /locus_tag="WGO49_000412"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ0305566.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAVSDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIGELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILSELDDLIGKWAAGRDTDEGFGDFAIRTGI
                     VKPVLDAPVDFWDASKAVPIARA"
     gene            complement(15982..17796)
                     /locus_tag="WGO49_000413"
     CDS             complement(15982..17796)
                     /locus_tag="WGO49_000413"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ0305567.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKASDSLEAAGIQVRRAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDRRFEELGAKRLLERVDADLDFAAAADGWTGRIVARLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARAGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFARDFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            complement(17793..19079)
                     /locus_tag="WGO49_000414"
     CDS             complement(17793..19079)
                     /locus_tag="WGO49_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ0305568.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNLVYPGGNMPWYRGEPLLVIL
                     ENLPGADETGRSAADFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            complement(19118..20041)
                     /gene="cysD"
                     /locus_tag="WGO49_000415"
     CDS             complement(19118..20041)
                     /gene="cysD"
                     /locus_tag="WGO49_000415"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ0305569.1"
                     /translation="MTKTEPNNVQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPAEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            complement(20079..20813)
                     /locus_tag="WGO49_000416"
     CDS             complement(20079..20813)
                     /locus_tag="WGO49_000416"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ0305570.1"
                     /translation="MKIFKPALWKIPPIENGSETALAEKTETLKQRLHRIAGSHRDAR
                     FASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHAETLNLLDRIERVYPHMQIKRFQP
                     IREDALHYVESKGRFAFYDSVEARRECCRIRKTEPLDRAIAGADAWLTGQRREQSATR
                     TELPFAEYDAGRGIDKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCTRP
                     VKAGEDIRAGRWWWEDKNSKECGLHK"
     gene            complement(20829..22280)
                     /gene="cobA"
                     /locus_tag="WGO49_000417"
     CDS             complement(20829..22280)
                     /gene="cobA"
                     /locus_tag="WGO49_000417"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0305571.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGGRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLSELAETAPNPALIVIGEVVGLHEKL
                     AWFGENAKKESNPAEHAYFALDGLGTGQEQQAA"
BASE COUNT         4853 a         5824 c         6457 g         5316 t
ORIGIN      
        1 tataaaatcg gcatttcgga tgtaagcaat tttgaaggca gaatgcgcca tttggaaaac
       61 aacggctatg cgaacgttgc cggattggaa cgcatcctcg ccgtcaaaac cgacaattac
      121 aaagaaaaag aaaacctgct ccatgaaatt ttcagcaaaa gcaggatagg cgataccgaa
      181 ttgttcgccg tggacgaaaa ccttgtaaaa cgtttgtttt tatcgcttcg cggcgaaatc
      241 gtgtttccga aaaacgaaac ggcggaatcg gaatttgaaa aaagcgtcca cgaacgcagg
      301 caggaaggga atgccgggtc cggccgcaaa caactgcttg atttggtacg gcgcggacac
      361 cgggaatacc cttacgcgct gccccggctt ttggcgggcg cggcatccta caagccgaaa
      421 aaatcgaaaa tccgcctttt taaagaagca tatttcggca aaagcggcac gaggctgacc
      481 gacgaaattg cagacggcat ccatatttac acctgttttt cgcgggcgga tttggaaaaa
      541 gcctattccg aatatttgga acttttcaaa tccgaatcgg atgccgaagg cagaaagccg
      601 cagtaaggtg caaacagata ccgtacacgt tgaggagcag atatgatggg cgattccgtc
      661 atttattatg tagaacaggc agacaaaccg gtaaaccgtg ccggcgaacg cgcccgcaaa
      721 acattcaaat atttttggcg cgagcttttt tgggaacgcc gccgcatcat tcccgcctta
      781 gattttgcca tggtcaaagc cccttttttc caagacggcg aagacggcga aatttgcgaa
      841 catatgtgga tcgacgatat ttattttgac ggtctttaca tttatggcgt gctgaacaat
      901 gaacccggcg aactgaccaa tgtcgaacaa ggcgaaagcg tttgcgttcc ggttgacgac
      961 atcagcgact ggatgttcgt gtgcaacggc attccctacg gcggctttac catacaggca
     1021 atgcgtggac agatgacgga agaggagcgt accgaacacg atgccgcatg gggaatcgat
     1081 ttcggcgatc ccgggcaggt attgctggtg tatgaagaaa aagaacatcc cgaaaatctg
     1141 gaagagcatc cgatgtgccg gaactgtatt gacgattttc ggcaacagtt gtcccaaaat
     1201 ccggattttc tgcatgagca ggacgaagac ggctatacgc cgcttcatca tgaagccatc
     1261 gcaggaaatg cacttatggt tcaagccatg cttgaatacg gcgcaaatcc tgcctcaaag
     1321 acatcggaag gctataccgc cctcgatttt gcccgcctgg cgggctggca aaatgttgcc
     1381 gacctgctcg aaccgcgaca ttaggcggac agttttccga aaacgaacac aaacactttt
     1441 tacagaaaga caataaaaat gcccaaaatc accgtacttc cacacacgac attatgcccc
     1501 gaaggcgcgg ttatcgaaga cgcgcccgaa ggcaaaaccg tccttgacgt gctgctcgac
     1561 catgatatcg aagtcgatca cgcctgcgaa aaatcctgcg cctgcaccac ctgccacgtc
     1621 atcatccgca aaggtttcga cagtttggaa gaaccgaccg aattggaaga agacctgctc
     1681 gaccaggctt ggggtttgga agccgattca cgcctgagtt gtcaggcggt tgtcgccggc
     1741 gaggatttga ttgtggaaat ccccaaatac accatcaacc acgcgcgcga agaacactga
     1801 aaacaggccg tctgaagccg gcacgcttca gacggcattg ttgcgcggat aaggcgcaat
     1861 cgcccgaaaa caggcgttcg tacaggcgga actttcgatt ctatagtgaa ttaacaaaaa
     1921 tcaggacaag gcggcgagcc gcagacagta ccaatagtac ggcaaggcga ggcaacgctg
     1981 taccggttta aatttaattc actatatatt gatttttatc ggttttctga cggaacggat
     2041 tggtgcggca tccgaggcgg attactcggg cgcgatgcac gggtatttat cggttttgca
     2101 gccggaaaaa ccgccggcgg gttactttca gggcggcggt cgggcggtat gccggatagc
     2161 gttccacttt cgcttctata ttgattttga ttggttttct gacggaatga tccgatgcgg
     2221 catccgggac ggcttgtgtt tcttcctgcc cgcctgccgg atattcctgt acttgcacga
     2281 aaccgaaaga ggtggcggtg cggcggacaa gctcgagata gcgtccttca agctccggac
     2341 aggcggcgga cacgctttct gccgtaaccg tgaagccgcc gcccgacccg gcaaggcgtt
     2401 ccgcaatctg cgtgtagatg cgccaacgtt gggcgcgcaa cattgccgca tcgcaaccgg
     2461 cagccgcttt atccaaggca atcagaccgc gcctgctccg gtagtgaagg caaaccgtta
     2521 ctctggagag gatatgttgc ccgtccaata tttgatacag tttgcgtatc agcagaatca
     2581 ggcggtcaaa ctcctccatc tccgacaggg aatcaggatg gtcggaagcg gtataaacca
     2641 gtttggacaa ttttgcggca aacatattgt tcatcaatct tccttgtcgg ttgaaacccc
     2701 gctcttcggg gcggtagaat cagatttgtt tgggagggac aactcctccc agatcaggac
     2761 gacacatagg ctggtgcttg atgtgttgtc cggcgagttg aaacattcag ccatcctcaa
     2821 ggtgcggcag ttttgccgaa acatattcta cacggcttca atgccggacg ataaaaggaa
     2881 attcatatga aatggaccga cacccggcgt atcgccgaag aactctatga cctgcacggc
     2941 gaaaccatcg atcccagaac cgtgcgcttt acccaactgc gcgacctgat tatggcattg
     3001 cccgaatttg acgacgaccc cgcccgttgc ggcgaacgca tcctcgaagc cgtgcagcag
     3061 gcatggatag acgaggcgga ataagtttcg ggaatgccgt ctgaaatgcg gcggtacgcg
     3121 gttcgtgctt ctgtttgcag cgggaatggt tttaccagtc tccttttttc agcctgtcca
     3181 gttggcggcg gtcgcgcttg gtcggtctgc cgtcgggata ggcggaagtg atgcggctga
     3241 attggtcgag ctgtttgcgc tcttccctca atgttgccgt tttcgcgtcc tcttcataca
     3301 gaagccgcgc ctcggatgcc gggcggcgtt ggtggttcaa acctttaacc ttgattttat
     3361 agggaaggga attgagcgtc aggtcgataa tatcgccgat gtctatggtt ttactgtttt
     3421 tgaccttcga gccgtttact tgaaccctac ccagttcgat gtgcttttgc gcaagggaac
     3481 gggtcttgaa aaaacgtgcc gcccaaagcc atttgtccag ccgcatggcg gaagaatcgt
     3541 gcttgtcttt catacgattt tgtttgaaat aattgaattt gtttcaagtt tagcataaga
     3601 tacgccgcct ataactagta tatatgcact aatccactgt tttccatgct gtccgaacac
     3661 aaaaagaggg tatggaaaag ccgttttgga caataaatta actgcggaat atgcacaaat
     3721 agcgtatgat agcggcagaa tctgttgatg agagcttcat tctatgaaac ctgttttttt
     3781 ggattttgaa caacccatag ccgaactgac caacaaaatc gatgagctgc gtttcgtcca
     3841 agacgagtct gccgtcgata tttcggacga aatacaccgt ttgcagaaaa aaagcaacga
     3901 tttgaccaag tctattttca gtaaacttac acccgcccaa atttcacagg tttcccggca
     3961 tccgcagcgt ccctatactt tggattacat tgaggcactg tttaccgatt ttgaagaact
     4021 gcacggcgac cgccactttg ccgacgatta tgcgattgtc ggcggattgg cgcgtttcaa
     4081 cggacaaagc gtgatggtcg tcgggcatca gaaagggcgc gacaccaaag aaaaaatccg
     4141 ccgcaacttc ggtatgcccc gtcctgaagg ctaccgcaaa gccctgcgcc tgatgaagac
     4201 ggcagaaaaa ttcggcttgc ccgtcatgac ctttatcgat acgccgggcg cgtatcccgg
     4261 catcggcgcg gaagaacgcg ggcagtcgga agccatcggc aaaaacctgt acgaactgac
     4321 acgcctgcgc gttcctgttt tgtgtaccgt catcggcgaa ggcggttcgg gcggtgcgtt
     4381 ggcggtcgcc gtaggcgatt acgtcaatat gctgcaatat tcgacctatt ccgttatttc
     4441 ccccgaaggc tgcgcgtcta ttttgtggaa aacggcggaa aaggcggcgg atgcggctca
     4501 ggctttgggc attaccgctg accggctgca aaggctggac ttggtcgata ccgtcatcaa
     4561 agaaccattg ggcggtgcgc atcgggattt cgggcaaacc atgaaaaacg taaaagccgt
     4621 tttggaaaaa caactgcacg aagcgcaaag catcccgctt gccgatttgc tttcgcgccg
     4681 tttcgaccgc attatggctt acggcaaatt ttcggaacaa taattcaggt agaacaagca
     4741 gcaagcagtt tgtctgaaac tgcttgcttt ttctttatcg ggacggaacc gtgctgactt
     4801 tagatgcgtt tgagcaatgc ttgaaggatt gttttcctca aggtctgaat ggaaaaaaaa
     4861 cagcggtggc attaagcggc ggcttggatt ccgtggtctt gctgcacctg cttgtccgcg
     4921 ctgggaaacg ggcaggattt gtgccggagg cattgcatat ccatcacggc ttgagccccc
     4981 gtgccgacga ttgggcggat ttctgtcaaa actattgcga tatgctaggg gtggggctgg
     5041 aaacggttaa ggtctgcgtg gaaaaaaacg gtttgggcat cgaagcggcg gcgcggcaaa
     5101 agcgttatgc cgcgtttgcc gaaaaaggct ttgacatttt ggcgttggcg caccacaggg
     5161 acgatcaaat cgaaaccttt atgctggcgg tcgcgcgcgg cggcggtttg cgcgctttgg
     5221 cggctatgcc cgccgtccgc ccttttgggg aaaaaggcat catttggcgg cccttgctgc
     5281 ctttttcgcg ccaagatata tgggattatg cccaaaaaca cggtttgccg aatatcgagg
     5341 atgaaagcaa taccgatacg gcttatttgc gaaaccgctt ccggcaccgt attttgcccg
     5401 aactttcggc gcagattccc catttcgggc gacatgtgct gaacaatgtc cgcgctttgc
     5461 aggaagattt ggctttgttg gacgaggtcg tcgttcagga ctgccgttgg gtttgcgggg
     5521 ccggttattt cgatacggcg cggtggctga cgttttcccc gcgccggaaa acccatattt
     5581 tgcggcattt tctgaaggaa aacggcattc ccgtgccgaa tcagaatgcc cttgccgaca
     5641 ttgcccgggt tttgacggag gcaaaaaccg gacgttggaa cttgcaaggc tttgaattgc
     5701 atcattatgc aggcaggctg tttgtgttcc gacaggaaaa aacggataaa ctgcggtttt
     5761 tgaaagacag gcagataagc ggaaatttaa gggaaatatt gacggggcag ggatttgttt
     5821 taaaacgtca tccgtttggg cttcctgagc atcttttgga gcaggacgga attttgagga
     5881 cggtagcggc atcggatacg ttggctgtcg gcggcatcca taaggatgtg aaaaaaatcc
     5941 ttcaggggaa acgggttttg cctgtcctgc gcccaatttg gccgcttgtt gccgacagcg
     6001 gaaaccgtcc attggcgttg gcaaactgtt gtgcggattt ccaatattcg gtttcagacg
     6061 gcattttgcc cgtccatcct gactttccca ttttattttg ataatatcgc aaacagattt
     6121 cggcggcatt cagtcgggta ttgtccggtt gcatatttct aaaaggcttg tgaagtgaaa
     6181 cacatcagtt cgaccaataa tgaacacatc cggcacctgc accgcctgtt gtcgcaagga
     6241 aagttcagac ggcagtatgc ccaaaccgtt ttggagggcg tgcacctgct tcaggttttc
     6301 ctgcaatccg gcgggatgcc ggtcggggta tatattcccg agggcaaaat gccgtctgaa
     6361 gaaatccgca ggctgagggc ggttgtgccg gaaggcagga ttttttccgt ttcagacggc
     6421 atattgaaaa aaatcagcag cttgagttgt gcagacgata tacttgcact gattgatatc
     6481 ccattgggcg gaaccttgcc ggacaaaggc gactgtgtgg ttttggacgg cgtgcaagat
     6541 ccgggcaatg tcggcaccgt cttacggagt gcggcggcgg caggggtagg tacggtcgtt
     6601 ttgggcaggg gttgcgcgga cgcgtggtcg cccaaggtat tgcgcgcggg gatgggcgcg
     6661 catttcttgt cgggcattta tccgcaggcg gatttggaaa tatggttggc gagctataaa
     6721 ggccgtgtgt ttgccaccgc cttgcgcgag gaaaagcagg tggttttgta cggcgaagat
     6781 ttgtgcgaac cgacagcctg ggtgtttggc aacgaaggcg cgggggtcgg taaagcggtt
     6841 ttggacaggg cggacaagtg tgtcaggatt ccgatgcacg atgcaaccga gtctttaaat
     6901 gtcgcgatgg cggcgacaat ctgcctgttt gaacagatgc ggcagcggtc ggcgtattga
     6961 ggaaaagaaa tgccgtctga aaaaatctat tacggcgtat tgattttctt atgtatcgct
     7021 tctatgctgc tgtcgccgtt tttttatgcg ggtgctttga agcccaagaa ggcggcattg
     7081 cggaaggacg ggcagtggaa actcatctta ttgtccaata ccgtggcggc ggcggttttg
     7141 ttttggatgt ggtggaaatg gttttgacag atattgctca aaatcgtgct aatggaatcc
     7201 gaacaaataa agaatttggt aaaaaatttg ttaaatcaac gaattaaagt tttgtggaaa
     7261 acaaaacagc tctaagcaaa tagggcgttt gtcggtaaat acggaagagt tgcggcatta
     7321 tcgggcatct ttaacaagta gtgccggctt ggcaggcaat tggtttttat gggcagtttg
     7381 caaaattgca gatataaaat tgcgaatcgg ttaaagcgtg aggataccat gaaaaattgc
     7441 aaattttttt atgatccgac aagggcaatc tatgatagcg gtgcagatta cttaactagg
     7501 gaaaaacata gattagtcgt tatcgcaaat agtgcttggg ggctattgct taatttatct
     7561 tgttattatg acgaggtttt ggaaaagcgg aaaataccgt tcggcaaaca ggaaattgat
     7621 gacgatatgg acaaagtgtc cgcccataag cggaagttta aagatatttc tgaaatcaaa
     7681 gtaggggatg gttgggaata cccgttcaat tatgagcagg gaatgaaaga attagatgaa
     7741 gtgctattga aatacattcc cttttttgaa gaagaacgat aaaggaggtt gatatgcgcg
     7801 tatctaaaat aattggaagt atgttgcttg ttacagcggt tcagaccgta ttttcggcaa
     7861 atgtttacgc gtgccgccat aatggtaaaa ccagttacag ccaaactccg ggaaaacatt
     7921 gtaccaacgc gggtttgggg cgggacctgg tgtacagttc ggtcaggcct gccgtgaaag
     7981 acagggcgga agacgcaggg gctgacgatc atttggattc ggtgagggac gaacacgtcc
     8041 aaaatccgag agagaatacg cataaggatg cttcgaatac cggtgcaaag acgcattgat
     8101 tgaaaccgaa tcagccatga cggcgttgaa taatagaatt tgaacgatta gggaactctg
     8161 atgaaacaca tccacattat cggtatcggc ggcacgttta tgggtgggat tgccgccatt
     8221 gccaaagaag cagggtttga agtcagcggt tgcgatgcga agatgtatcc gccgatgagc
     8281 acccagctcg aagccttggg cataggcgta cacgaaggct tcgacgcggc gcagttggag
     8341 gaatttaaag ccgacgttta cgtcatcggc aatgtcgcca agcgcgggat ggatgtggtt
     8401 gaggcgattt tgaaccgcgg gctgccttat atttccggtc cgcaatggct ggctgaaaac
     8461 gtgctgcacc atcattgggt actcggcgtg gcggggacgc acggcaaaac gaccaccgcg
     8521 tctatgctcg cgtgggtttt ggaatatgcc ggactcgcac cgggcttcct tattggcggc
     8581 gtaccggaaa atttcggcgt ttccgcccgc ctgccgcaaa cgccgcgcca agacccgaac
     8641 agccaatcgc cgtttttcgt tatcgaagcc gacgaatacg acaccgcgtt tttcgacaaa
     8701 cgctccaaat tcgtgcatta ccgtccgcgt accgccgtgt tgaacaatct ggaattcgac
     8761 cacgccgaca tctttgccga cttgggcgcg atacagaccc agttccacta cctcgtgcgt
     8821 accgtgccgt ctgaaggctt aatcgtctgc aacggacggc agcaaagcct gcaagatact
     8881 ttggacaaag gctgctggac gccggtggaa aaattcggca cggaacacgg ctggcaggcc
     8941 ggcgaagcca atgccgacgg ctcgttcgac gtgttgctcg acggcaaaac ggccggacgc
     9001 gtcaaatggg atttgatggg caggcacaac cgcatgaacg cgctcgccgt catcgccgcc
     9061 gcgcgtcatg ccggtgtcgg cattcagacg gcctgcgaag ccttgggcgc gtttaaaaac
     9121 gtcaaacgcc gcatggaaat caaaggcacg gcaaacggca tcaccgttta cgacgacttc
     9181 gcccaccacc cgaccgccat cgaaaccacg attcaaggtt tgcgccaacg tatcggcggc
     9241 gcgcgtatcc tcgccgtcct cgagccgcgc tccaacacca tgaaactcgg cacgatgaag
     9301 tccgccctgc ctgtaagcct caaagaagcc gaccaagtgt tctgctacgc cggtggcgtg
     9361 gactggaacg tcgccgaagc cctcgcgcct ttgggcggca ggctgcacgt cggcaaagac
     9421 ttcgatgcct tcgttgccga aatcgtgaaa aacgccgaag caggcgacca tattttggtg
     9481 atgagcaacg gcggtttcgg cggaatacac ggaaagctgc tggacgcttt gagatagccc
     9541 gggcgatgcc gtctgaaagc ccttcagacg gcatcgtccg gctgcgcggc acaaaggcgg
     9601 aaaaaccgtt tgccccgtat tttcaaacgc gttacacttg ccgccgctgt tttcagccat
     9661 ttgattaccc gcaaccgccg tcattgcgcc ggcggtttgc ctgtcagcgt cattgcgccg
     9721 ctgtaaatac gaaagaacac attatgaccg tatcccccgt cgccttgcgc cgtaagaccg
     9781 agtgcaagcc tcatcccacc gcgcgctatt ggaaaaaatg cgatgtcgag gcacttttcg
     9841 gactgccgtt tttagaactt gtccatcagg cggcagaagt ccaccgccaa aatttcaacc
     9901 cgcgcgaaat ccagctttcc acgctgttgt ccatcaaaac cggcggttgt cccgaagatt
     9961 gcgcctactg tccgcaatcg gcgcaccaca ataccaatct gggcaaagag cagatgatgg
    10021 atgtggatga aatcgtcgaa aaagccaaaa tcgccaaatc gcgcggcgca agccggtttt
    10081 gtatgggcgc ggcatggcgc ggccccaaac ccaaagacgt ggagacggtt tccgcaatca
    10141 tcaaagccgt caagggcttg ggtatggaaa cctgcggcac gttcggtatg ctcgaagaag
    10201 gtatggcgga agacttgaaa gaggcgggct tggattatta caaccacaac ctcgacaccg
    10261 accccgaccg ctacaacgac atcatccata cacgccggca tgaagaccgt atggacacct
    10321 tgggcaaagt ccgcaacgcc ggtttgaaag tctgctgcgg cggcatcgtc gggatgaacg
    10381 aaacccgcgc cgaacgcgca gggctgattg ccagcctcgc caacctcgac ccgcagcccg
    10441 aaagcgtgcc gattaaccgg ttggtcaaag tggaaggtac gccgcttgcc gatgccgagg
    10501 atttggactg gacggaattt gtccgcacca tcgccgtggc gcggattacg atgccgcaaa
    10561 gttatgtccg actgtcggca gggcgcagca atatgccgga agcgatgcag gcgatgtgct
    10621 ttatggcggg cgcgaactcg attttttacg gcgacaagct gctgaccacg ggcaatccgg
    10681 atgaagacgg cgacagaatc ctgatggaaa agctcaacct gtatccctta cagtttgagc
    10741 tggaaggcga ggtcgccgaa gtggaaaaag cctctgggat taaagtggat tattgacgat
    10801 tgcaaaaatg ccgtctgaaa cccgaaaaaa ggctttcaga cgacatttgt tcggacggta
    10861 tttccaatat ctttttaccg gcgcgtgatg ttgccgtcgg gcgagacatc cagcccgttc
    10921 ccgttgggga agtcgctgcg gttcagataa ataatccgcc cgataacggt ttttcagggt
    10981 cgattttggg gattttatcg tcggcttggg taatagggat gatagttccg gaaataaatt
    11041 taccctgttt gttggtgata attttgaaaa tcggcgcaat gccgctgatg ccggaggtgt
    11101 tgattgcgcc gtaggtggca aagttgccgc cgctgtaaga gatgaagcgg tcgcggtaaa
    11161 gttcgacggc gcgtgtaacg tgcggcccct gcccgaatac gacatccgcg ccggaatcga
    11221 cggcaagccg tgcaaactcg acgacgttgc ccctgttttc cccatagaag atttcggtat
    11281 cgaacggcag gtgttccgcc tgtttccctt cccgccgccg tggaacatta caatgacgat
    11341 gtcggctttc tgtttggttt tggtaatccg ttttctaact ttggcataat cgttcagttt
    11401 gacggcggca aggttggggg cgaaggagac gaagccggat cttacgccgt ttttcttcag
    11461 gatggcggtt tcaaacctgt tttcgatgcc cgaatatttg atgttcaatt cgtcaaggtt
    11521 cgccctttat gccgtttccg caccgcaaac cgccggggtc aagccctcgg cgcatcctgc
    11581 cggaacggaa tccccgtgct gccgattgac ggttcaaacc ccgcgcccgt ttcaaatacc
    11641 ggcgatgtgg acggacagga tgcgcctgac gaaaagacag ccgataccgt ttccattatc
    11701 ggcgtgggcg acattatgct cggcagcaat tatccggtcg attacctgcc cgataccaat
    11761 attctgaaaa acgtcgaatc tgccttgcaa gacgcggaca ttaccgtcgg caacctcgaa
    11821 ggcacgctgt ttgacgaagg cggtacgccg aaaaaatgtg caacccccca aaatatgcta
    11881 tgcattccga acgccctccg catacgggca ataccttgcc gacgcgggat tcgactacct
    11941 cagcttcgcc aacaaccaca gcaacgactt cggcgcgcaa ggcatcacgg caacggcggc
    12001 gagcggcagc tcttttacat actcgatcgc gctaaagccg ctgccgataa cgaggccaaa
    12061 attgcggaaa ataccgccat cgcccagata aatttgtcca tcatcagacc tttactgttc
    12121 agacgagaca gcatttgccg cacgttttgg ggcttatctt tcgatttgcg ctacgtcgcg
    12181 caccgcgcct ttgtcggcgg aagtcgccat cgcgccgtaa gcacgcaggg ctgcggagac
    12241 gtagcggtcg cggttttccg gtttccacgc tttgctgccg cgcgcttcca tttcggcacg
    12301 gcgggcggca agctcttcat cggaaatggc aaggtggatg ctgcggttgg ggatgtcgat
    12361 ttcgacggta tcgccttcgt gtaccaaacc gatcgcgcca ccttccgccg cttcgggcga
    12421 ggcgtgtccg atggacaaac ctgatgtgcc gccggagaag cgtccgtcgg ttaagagagc
    12481 gcaggctttg ccgaggcctt tagatttcag gtaggaagtc ggatacagca tttcctgcat
    12541 gcccgggccg cctttcgggc cttcgtagcg gatgatgacg atgtcgccgg cgacgatttg
    12601 gttgcccaaa atgccttcta ctgcgtcttc ttggctttca aacacgcggg cgcggccggt
    12661 gaatttgagg atgctctcgt ccacgcctgc ggtttttacc acgcagccgc gctcggcgat
    12721 gttgccgaac aagaccgcca aaccgccgtc ttgcgagtag gcgtgtgcca cgtcgcggat
    12781 acagcctttt tcgcggtcga ggtcgagggt tttccacata cggttttgcg agaacgcttg
    12841 ggtggtgcgt acgccgccgg gcgcggcttt gaagcgttcg atggcgcggg tgttttcagg
    12901 gttggttatg tcccattttt caatcgcgtc tttcagcgtc ggtgcgtgga tagtgtgcac
    12961 gtcggtgtgc agcttgcccg ctttgtccaa ttctttcagg atggcgaaaa tgccgccggc
    13021 gcggtgcacg tcttccatat agtagtcgtg gttgttgggt gcggttttgc agatgcaggg
    13081 cacaacgcgg cttaagcggt caatgtcggc cattttgaaa tcgacaccgg cttcgttggc
    13141 aacggccaac aggtgcaaaa tggtattggt gctgccgccc atcgcaatat ccatcgtcat
    13201 agcgttttca aacgcttttt tggtggcaat gctgcgcggt aacacggttt catcgttttg
    13261 ctcgtaatag cgtttggtga tttcgacaat catacggccg gcttcgagga acagttcttt
    13321 gcggccgacg tgggtcgcca aatacgaacc gttgccgggt agggacagac cgagtgcttc
    13381 ggtcaggcag ttcatcgagt ttgccgtaaa catacccgaa cacgagccgc aggtcgggca
    13441 ggcgttttgt tcgacttcct cgacttgccg gttgctgaca ttgtcgtccg ccgattcaat
    13501 catcgcgtca atcaagtcca aacggcgttc gggctggatg tttgccacgc cgataacttt
    13561 acctgcttcc atcggaccgc cggaaacgaa gatggtcggg atgttcaggc gcatcgcggc
    13621 aatcagcatg cccggggtga ttttgtcgca gttggaaatg cacaccagcg cgtcggcgca
    13681 gtgggcattg accatatatt cgatggagtc ggcaatcaaa tcgcggctgg gcagggaata
    13741 cagcataccg ctgtgtccca tagcgatgcc gtcgtcgatg gcgatggtgt tgaattcttt
    13801 ggcgatggcg cccgcctttt cgatttcgcg ggcaaccagc tggcccatat tgtgcaggtg
    13861 gacatggccg ggcacgaatt gggtgaagga gttggcaacg gcgatgatgg gcttgccgaa
    13921 gtcggtttcc atcacgccgg tggcgcgcca caatgcacgt gcgcccgcca tattgcggcc
    13981 gtgggtggag gttttggagc ggtattctgg catagtgtgt ttccttgtct gctatgccgt
    14041 ctgaacggca aaaggctttc agacggcatt tttgttgtgt tttgaaatga aaaaaaaggt
    14101 attttatacc aagtatcgga attttgcgga atggaaacgc gtgcggcaaa aaaggacatc
    14161 ccccgcagga atgcggggga cggtttcagg cgcgcgcaat cgggacggct ttggatgcgt
    14221 cccaaaaatc gacgggcgca tccaacacgg gtttgacgat gcccgtccgt atggcgaaat
    14281 cgccgaaacc ttcgtcggta tcgcgtcccg ccgcccattt gccgatcagg tcgtctaatt
    14341 cggaaaggat ttccggcagg gtaatgtttt ctttgtagag tcgggggatg cgtacgcctt
    14401 cgcggtcgcc gccgatgtgg aggttgtagc gtccgacggc tttgccgacc agtccgattt
    14461 ccgccaacat cgcccgtccg cagccgttcg ggcagccggt aatgcgggta acgatgtagt
    14521 cgtccgacgt gccgtgtttc gccataatct tatccagctc gccgatgaag tccggcagca
    14581 cgcgttcggc ttccgccatt gccagcgggc aggtcgggaa ggaaacgcac gacatcgcat
    14641 tttcacgcag tttactgaca tcgttgcgga ttaatccgta tgttcgggca agttcttcga
    14701 tttttgcttt gtctgcttcg gcgacatttg ccacgatgag gttttggttg gcggtgatgc
    14761 ggaaatcgcc tttgtggatt ttggcgattt ccaacacgcc ggtcagcagc tgtttcccgc
    14821 cttcgtcaac caaacgcccg ctttcgatga aaagggtcaa atgccagttg ccgtctatgc
    14881 ctttcaccca gccgatacgg tcgccgcgcc cggtaaattt gaacgggcgt atgggttcga
    14941 acggcatacc catacggcgt tcgacttccg cgcggaagtt gtccaagccc atattttgaa
    15001 tggtgtagcg ggtgcgggcg tttttgcggt cgctgcggtt gccgaagtcg cgctgcgtgg
    15061 ttaccaccgc ttcggcggcc ttcagcgcgt gttccggagg cacgaaaccc agttccagtg
    15121 aaatgttcgg ataggttttg gtgttgccgt gttccatcga aagcccgccg cctgccaaaa
    15181 cattgaagcc ggcaagctgt ccgttaccgt ctgaaacggc gacgaaatcc aaatcgttgc
    15241 cgtagcagtc cacatcgttc aagggcggga tgacgactgc ggttttgaat ttgcgcggca
    15301 gataggtttt gcccaaaatc ggctcgtctt cttgaaggaa gtcgtcggaa ctttgaactt
    15361 ttttgccgtc cacccacaca tccagataac cgcgcgtgcg cggcagcaag tgttcggaaa
    15421 tctttttcgc gtattcgtaa gcctgccggt gcagttcgga ctcgatcggg ttggacgtgc
    15481 aaagcacgtt gcggttcata tccgccgccg tggcgatgga atccaaaccc agtttgtgca
    15541 agaggcggtg catcgtctgc aacttggctt tcggcacgcc gtgaaattgg aaggtttgcc
    15601 ggttggtcag ccggatggag cggtaatgac tgttttcccg ggcaaatttg tccagttcta
    15661 tccattggga cggtttgatg atcccgcccg gcagccggca gcgcaaaagc ataaatttca
    15721 agggctcgag ttttgcctcg gcgcgttcgg cgcggatgtc gcggtcgtcc tgctcataca
    15781 taccgtggaa gcggatgagt tggaagttgt cgcctttgaa gccgcccgtg agcgggtctt
    15841 tcaaatcgtc caaaatcgtg ccgcgtaaaa aattgctttc ggttttcaga cgttcgttgt
    15901 cggatagcgg tttttcttgc cacgccaaac cttttgtctt ggtctgtacg gtcattttgt
    15961 gttcctcccg attatattta atcaataaac atcacgctga tagcgttttt cttcgcgcag
    16021 catatccaaa tattcttctg cgccctcttc gtccaaatgt cctgccccga taatcacatc
    16081 cagcaaggcg gcttccacgt cttttgccat ttttgccgca tcgccgcaca catagatatg
    16141 cgcgccttcc tgcagccatt gccaaagtcc ttccgcctgt tcgcggattt tgtcctgcac
    16201 atagattttt tcttcctgat cgcgggacca ggcgaaatcg tacctgtgca ggaaaccgtc
    16261 tttggcaaac tgctgccatt cggtttgata gagaaaatca cgggcaaaat gcggattgcc
    16321 gaaaatcagc cagtttttgc cttccgcatt ttctgcggca cgttgttgga cgaaggcgcg
    16381 gaacggtgcg acgccggtgc ccgagccgat catcacaatc ggcttgcggc tgtcttcggg
    16441 cagcctgaag ccgtcgttgc gttccacaaa cacgcgcacc gtgccgtcct cttccagccg
    16501 gtcggcaagg aaacccgatg cgccgcccgc cctggcgcgc ccttcgtgtt cgaaacgcac
    16561 cgcgccgacg gtcaggtgca cttcgtcccc cacttccgcc tgcgacgagg aaatcgaata
    16621 caggcgcggc gcaagcgggc gcagtaggcc ggcgaattgt tccgccgtca gttttgccgg
    16681 gaagcggtgc agcacatcgg caatcggcgt gctttgcaca aagccttgca aaacggcgtt
    16741 gtcggcagca atacggtcga gttcgtcatc atcggcgaac ggggcatagc ctttgacaaa
    16801 ggcgggggtg ttttgcgtga gttcaaaatg ggataacagt gcggaggcaa ccggcagggt
    16861 ttttccgccc gcctgtattt ccgttgccgg atcgatgccg agcaggtcta ggatttccct
    16921 gaccagtgcc ggatcgttgt caaaccaaac gccgagcgcg tcgcccggga ggtagtgcaa
    16981 atccgaaccg ctcaaatcga tttcgatgtg gcgcacgtct ttatcggatt ggcgggcggt
    17041 gattttctga ttggccagca gggcggcggg aaaggggtct gccttgcagt acctgccatc
    17101 cggtgccgtc tgaaggccgg cggggggcgt tgtctgcggc gcgggcgttg cccggttttt
    17161 tgcggcttct tcttttaagc gcgcgacaat gcggcctgtc catccgtctg cggcggcggc
    17221 aaagtccaaa tccgcatcaa cgcgttcgag caggcgtttt gcgcccaatt cttcaaaacg
    17281 ccggtcgaaa tctttgcccg cccggcagaa attcggatag gaactgtcgc ccaaacccag
    17341 tacggcaaat tggagtttgt ccaatttcgg ggcttttttg ccgttcagca gtttgtgcag
    17401 cacgacggct tcttccggcg gttcgccttc gccctgggtg gacgtaacca gcagcaggcg
    17461 gcgttcgtcg gcgatgtttt tcgccttata gtctttcagt tcggcgcgcc tgacttggat
    17521 gccggcggct tccaggctgt ccgacgcttt gtcggcaacg gatttcgcat tgccggtttg
    17581 cgaggcggaa aggacggtta cggaaaaagg ttctgccgcc ggcaatgccg tctgaagcgc
    17641 gggcagccct gcggatgccc cgtttcctgc ttttgcccaa gcgtagccgg acagccacgc
    17701 ccattgtgcc gcgtccagcc ccgacaggag ctgcgtgatt tcgggcggca gaggcggtaa
    17761 tggcggattt gtgttctgca tatcgtgttc actcataaaa tcatacctgc cgcaacagtg
    17821 ccgtatgtcg cttcgtctat caggataaac gaaccggcgg cggtgttttc cgcataaggc
    17881 gttgccgtaa cgggtttttg aaggttgatg cggactttgg cgatgtcgtt catcttcaag
    17941 gattccgcgc cggcctcttg ttccagcgtg cggacatcta aaacgctttc aatttccccg
    18001 acttttgccg gcacggtttg cgtgccgtgc ttgagcaggt atttgcgcgc ggtgttgagc
    18061 ggacgttcgt caaaccagca aagcgtggct tccagatgtt tttgcggggc gagcggggaa
    18121 tttttatcga caaaaaggtc gccgcgcgaa acatcgatgt cgcggtccag ccggagggtt
    18181 gccgcctcgc cggcaaaagc ctgcgccact tcccctttcg gcgtgatgat ttcggacact
    18241 tcggcggtca gcccgttcgg ttcgatgcgg acggtttgcc cgacggcgac cgaaccgcgt
    18301 tcgatgcgcc cctgatagcc tcggaaatca tcggccttgt cggcatcttg gcggacgacc
    18361 agttggacgg ggaaataaaa atcggcagcg gaacgaccgg tttcgtccgc ccccggcagg
    18421 ttttccaaga tgaccagcag cggttcgccc cgataccacg gcatattgcc gccggggtaa
    18481 acgaggttgt cgcccaagag tgcggacacc ggtacgaaat gcacgtcttt caaaccgagc
    18541 tgttcggcaa gtcggcggta tgcctccaca atggcgttga atttgtcttc gctgtaatcc
    18601 agcaggtcca ttttgttgac cgccaccaca atatgcgggc agttgagttg gcggaggatg
    18661 gcggaatggc gtttggtctg cggcagaagc tgcaagggct gcgcgccgaa atccagttgg
    18721 gatgcgtcaa ccagcacgac tgccgccgaa gcggtgcttg cgcccgtaac catattgcgc
    18781 gtgtattgtt cgtgccccgg cgtgtcggcg atgatgaatt tccgtttcgc cgtggaaaaa
    18841 tagcggtatg ccacatcgat cgtaatgccc tgttcgcgtt cggcttccag tccgtcggtc
    18901 aggatggaga agtctatggt ttctttcaaa cctttgcttt tgccggattc caaggttttg
    18961 atttggtcgg acagcagggc tttgctgtcg tagagcagtc gtccgatcag ggtgcttttg
    19021 ccgtcatcga cgctgccggc ggtaatgaag cggagcgggg cctggtgttg tgccgtcata
    19081 ttttcttcct catatctgct taaagggttt ttgaaattta gaaatagcct tcttttttgc
    19141 gtttttccat tgccgcctcg cttgcctgat cgtctagccg ggtcgcgctg cgttcggaaa
    19201 tgtcggcaac cgccgtttcc ctgataatct ccgccggcgt ggacgcggtg ctttctaccg
    19261 ggcaggtgca gctgatgtcg ccgacggtgc ggaagcggac atcaaggatt tcggaggttt
    19321 cagacggcat tttcggggtg agcggcgtta cagggaccag cagccccctg cgtctgacca
    19381 cttcgcgcct gtggctgtaa taaatcggcg gcagctcgag gttttcgcgg gcgatgtatt
    19441 gccagatgtc gagttccgtc cagttggaaa tcgggaagac gcgcatattt tcgcctttgt
    19501 gcagcctggt gttgtacagc gaccacagct cggggcgttg cgccttcgga tcccattgtc
    19561 cgaactcgtc gcggaacgag aaaatccgtt ctttggcgcg ggctttttct tcgtcgcgcc
    19621 gcgcgccgcc catcagcgcg tcaaagccgt ttgcctcgat ggtttccaac aaggtaaccg
    19681 cctgtgccgc attgcgcgaa tcggtttctt tgcgtaagac caccgtgcct ttagcgatgg
    19741 agtcttccac gcgccccact atcaggcggg cattgagttt tgccgcctgc gcgtcgcgga
    19801 aggcaatcac ttcggggtag ttgtgccccg tgtcgatatg caccagcggg aagggcagtt
    19861 ttaccggcct gctgcccagc cggaaggctt tgcaggcgag ggcgagcagg accacggaat
    19921 ctttgccgcc ggaaaagagc agggcggggt tttcgcattc tgccgccact tcgcggataa
    19981 tgtggatgga ttcggattcc aaccagtcga gttggacgtt gttcggttcg gttttcgtca
    20041 tacgatattc cttatttctt ctgtttgatc cttatgaatt atttgtgcag cccgcattct
    20101 ttgctgtttt tatcttccca ccaccaccgc ccggcgcgga tgtcttcgcc ggccttgacg
    20161 gggcgggtgc aggggtcgca gcctatactg ggaaagcctt gccggtacaa atcgttgtaa
    20221 ggcacattgt tggcaaggat gtatgcccac acgtcgtgtt ccgaccagtc gaaaatcggg
    20281 ttgtatttat cgatgccgcg cccggcatca tattcggcaa acggcagttc cgtgcgtgtg
    20341 gcggactgtt cgcggcgttg cccggtaagc caagcatccg ccccggcaat ggcgcggtcg
    20401 agcggttcgg tttttcggat gcggcagcat tcgcggcgcg cttcaacgct gtcgtaaaag
    20461 gcaaacctgc ctttgctttc cacataatgc agggcatctt cccgtatcgg ttggaaacgc
    20521 ttgatttgca tatgcggata aacacgttca atcctatcca acaggtttaa agtttccgcg
    20581 tgcagcagcc ccgtatccaa agtaaaaata ccgatattga ggttttctcc ggcgataagg
    20641 tcggtaatta ccatatcttc cgcagcaagg ctgctggcaa accgtgcatc ccggtggctg
    20701 ccggcaatcc ggtgcaggcg ttgtttgagg gtttccgttt tttccgccaa agccgtttca
    20761 ctgccatttt caatcggcgg aattttccat aaagcaggtt tgaaaatttt cattttgtcc
    20821 gccttgcttt atgcggcttg ttgttcttgt ccggtgccca gtccgtccaa tgcaaaatac
    20881 gcgtgttcgg ccggattgct ttcttttttg gcgttttcgc cgaaccaagc cagtttttcg
    20941 tgcagcccca ccacttcgcc tatgacaatc aatgccggat tcggcgcggt ttcggcgagt
    21001 tcggaaaggt tggcgagcgt gccggttgcg gttttttgag ccggcagcgt gccttggctg
    21061 ataacggctg ccggcgtgtc gggcgagcgt ccgtgctgtt gcagccgttc ggcaatcagg
    21121 gcggctttga gcgcacccat ataaatcacc aaggtctggc ggctgcgggc gagggtctgc
    21181 cattcgatgt cgggcgcatc cgccttgcgg tggccggtta cgaaaaccgc actttgggca
    21241 taatcgcggt gcgtgagcgg gatgccggca taggcggtcg cgccgacggc ggcggtaatg
    21301 ccggggacga ccgaaaacgg gatctgatgg cgtgccaagg tttccaattc ttcgccgccg
    21361 cgtccgaaca cgaaagggtc tccgcctttt aaacgcacca cgcgcctgcc ttcgcgggcc
    21421 agcctgacca taagcgcatt ggtgtcctct tgcggggtgc ggccgccccg ggcgcgcttg
    21481 ccgacaaaaa tccgttccgc atcgcggcgg acgagggaca gtatgccgtc tgaaaccagc
    21541 gcgtcgtaaa gcaccacgtc tgcctgctgg atttcctgca gccctttgag cgtcagcagc
    21601 cccgcatcgc cgggacccgc accgaccaaa gacacaaaac cgccttgata attttgttcc
    21661 aactgctttg ccaactcctg ttcggcttgg gcggtttgtt gatttttaac gagggcggca
    21721 aaacggccgt tgaactgctt ttcccaaaaa cggcggcgtt cggtaacgga tttcagtttg
    21781 gctttgaccg catcgcgcca tcttccggag atttccgcca tatcgcccaa agacggcggc
    21841 agcagggctt ccagcctttc acgcagcagt cgggcgagga cgggcgcgct gccggagctg
    21901 gaaacggcaa tctgaaccgg gttgcggtcg ataaccgacg ggaagatgaa gctgcaatgg
    21961 tcgcggtcgt ccaccacgtt gaccggcttt tggcagcttt cggcaagatg gaaaacgcgc
    22021 cggttgaggg cttggtcgct gcttgccgca atgatgagga aaaccgtgcg gatgtgttcg
    22081 gcacgaaatt cttcggcaag ccacaggatt ttgttttccg ccgccaacgc ggagagttcg
    22141 gcattcaggt gttttgcggc aaccctgacc tctgcgcccg ccttcagcag caggctgatt
    22201 ttgcgtgcgg cgaccgcgcc gccgcctacg accaatacgg ggcggccggc gaggttggcg
    22261 aaaataggga aataattcac tggctgactc ctttgctgtt tgcccgcacc ttgtttccga
    22321 tacggtgcgt cgcggcattt ttgtcggaat gcgggtcatt ttagacaaaa ggattttccc
    22381 ggttaaataa taaaaaggca tttgttagaa gctgaaagct atatgagggc ggctgcggat
    22441 gcggcggttt
//
LOCUS       DAWFAD010000014        22266 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWFAD010000014 DAWFAD010000000
VERSION     DAWFAD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.014"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(120..1034)
                     /locus_tag="WGO49_000418"
     CDS             complement(120..1034)
                     /locus_tag="WGO49_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0305572.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WGO49_000419"
     CDS             1290..2759
                     /locus_tag="WGO49_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ0305573.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WGO49_000420"
     CDS             3011..3382
                     /locus_tag="WGO49_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305574.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WGO49_000421"
     CDS             3455..4864
                     /locus_tag="WGO49_000421"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ0305575.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWTEVAAGKGFDLQFALDKVGGKYGFTSSKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="WGO49_000422"
     CDS             4910..5704
                     /locus_tag="WGO49_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305576.1"
                     /translation="MTLPMQETRFSILLDELAAKQKAAIAPHLLADGSKVWIRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRAARIAVPELLAVRK
                     NALMFGNLEGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADMPP"
     gene            5892..6668
                     /locus_tag="WGO49_000423"
     CDS             5892..6668
                     /locus_tag="WGO49_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0305577.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7336..9807)
                     /locus_tag="WGO49_000424"
     CDS             complement(7336..9807)
                     /locus_tag="WGO49_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ0305578.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNLRAGMDTPIAVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHRMPGYPAVQDVREAAVVKLQ
                     AGDVVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9804..10982)
                     /gene="hflX"
                     /locus_tag="WGO49_000425"
     CDS             complement(9804..10982)
                     /gene="hflX"
                     /locus_tag="WGO49_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ0305579.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSATRLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLVNLKKQRALRRKPRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIATVRISVAENTGIDALREAIAEYCATAPNTDETEMP
                     "
     gene            complement(10987..12267)
                     /locus_tag="WGO49_000426"
     CDS             complement(10987..12267)
                     /locus_tag="WGO49_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ0305580.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNSRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12420..13145)
                     /locus_tag="WGO49_000427"
     CDS             complement(12420..13145)
                     /locus_tag="WGO49_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ0305581.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13218..13895)
                     /gene="radC"
                     /locus_tag="WGO49_000428"
     CDS             complement(13218..13895)
                     /gene="radC"
                     /locus_tag="WGO49_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ0305582.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTSQSVRSF
                     RQLGLMP"
     gene            complement(14018..15367)
                     /gene="gshA"
                     /locus_tag="WGO49_000429"
     CDS             complement(14018..15367)
                     /gene="gshA"
                     /locus_tag="WGO49_000429"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ0305583.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNVYALGEILCSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     AAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNSAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15627..17036
                     /gene="leuC"
                     /locus_tag="WGO49_000430"
     CDS             15627..17036
                     /gene="leuC"
                     /locus_tag="WGO49_000430"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ0305584.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLTPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17133..17387
                     /locus_tag="WGO49_000431"
     CDS             17133..17387
                     /locus_tag="WGO49_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305585.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17449..18090
                     /gene="leuD"
                     /locus_tag="WGO49_000432"
     CDS             17449..18090
                     /gene="leuD"
                     /locus_tag="WGO49_000432"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ0305586.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPGGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18260..18632
                     /gene="dcm"
                     /locus_tag="WGO49_000433"
                     /pseudo
     CDS             18260..18632
                     /gene="dcm"
                     /locus_tag="WGO49_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            18685..19080
                     /locus_tag="WGO49_000434"
     CDS             18685..19080
                     /locus_tag="WGO49_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0305587.1"
                     /translation="METLEALGYYVSWKVLNAKDFGIPQNRKRIYLTGSLKSKPDLSF
                     ETSPSPKLKNILESGLPTESSPFIKKLLKKFPPSELYGKSVKDKRGGKNNIHSWDIEL
                     KGAVTEEEKQLLNILLKERRKKNGLQKSA"
     gene            19095..19529
                     /locus_tag="WGO49_000435"
     CDS             19095..19529
                     /locus_tag="WGO49_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0305588.1"
                     /translation="MPLTKAQISTFYKHPDLQNILDSLTDKGYLVLEHPKQKIGGQRI
                     KDESLPKGYNIVSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGL
                     RLFGYPDDYSFDIPKKDRCDLLGNTVAVPVIKAVSERLLHTL"
     gene            19545..19706
                     /locus_tag="WGO49_000436"
     CDS             19545..19706
                     /locus_tag="WGO49_000436"
                     /inference="COORDINATES: protein motif:HMM:NF021105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0305589.1"
                     /translation="MIKLTAQQIFDKLLDEEKILSANGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGG"
     gene            19707..20276
                     /locus_tag="WGO49_000437"
     CDS             19707..20276
                     /locus_tag="WGO49_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0305590.1"
                     /translation="MRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNTGPG
                     FDIADFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWA
                     INLQVKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKK
                     KFEEAYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20310..21380
                     /gene="leuB"
                     /locus_tag="WGO49_000438"
     CDS             20310..21380
                     /gene="leuB"
                     /locus_tag="WGO49_000438"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ0305591.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDAGYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAVLAAL"
     gene            21543..22106
                     /locus_tag="WGO49_000439"
     CDS             21543..22106
                     /locus_tag="WGO49_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ0305592.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQRPMEKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5364 a         5852 c         5793 g         5257 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa gatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccgcgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagt
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatgctc cagcttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatggtta tcaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc ggcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaggcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctagggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcag aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaaccgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ctttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggcggctg
     3061 cgccgccgga ggcggtaaca cattcggcag cttagacggt ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggtcg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgccga aaaacaggcg gagtgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccgaacg gatgaccggc aacgatgtga tgcagatgct
     3301 ggctccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatgc cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgggaaacc ttggacaaat ggcgcggttt
     3601 ggcttatccc gaattggcgt ttgaaattat gcgcctgttc gttaccgaca ttccggagga
     3661 cgatttgcgc gatattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg cgatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgtg cgcagatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggtatgtttg acgactgtca ggatattgtg aaggcggtgc aaaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg attaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgccggcta tttcaaagcg acgcaaagca atgacgagca
     4201 agtcagcttt tgcgtgccga gcggcaactt cggcaacgtt tgcgcgggac atattgccaa
     4261 acagatgggc ttgcccatcc gccgcctgat tgtcgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cgtaccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccatct atggacattt ccaaagcgtc caatttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtggacggaa gtcgcggcgg gcaagggctt
     4501 tgacttgcag tttgccttag acaaagtcgg cggcaaatac ggctttacct ccagcaagtc
     4561 cacccacgcc gaccgcctcg ccaccatcaa acaggtttac gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggtggtttgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatgttcc gtacaggggc actccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ccatactgct agacgaattg gcggcaaaac agaaggcagc
     4981 catcgccccc cacctgcttg ccgacgggag caaggtatgg atacgcaaag caggcaggca
     5041 caatgcgcga tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt gcaaccgaat caaaacgcct
     5161 gtacgaattg cgcgcggcga ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggcaatttgg aaggcatccc gctcgatacg caaatccgcc aagaagccga
     5281 agccggaaag gcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcgcg gaatatgatg tcggacggaa aaaacatcag
     5401 ctttctagat tttgaagacg acccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacatcc attcgaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtatt gtcggatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac cgatcctccc gatacgcagg ctggaacacc cacgctgggg
     5641 acgggacgca ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atatgccgcc
     5701 ataagccgcc gcccgccccg cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta tcccgaatcg
     5821 ggcagacatt tccactctgc cgcacctccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgcgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaactctggt tgccggcgac ctgaatccgg gcaaacacct ttacttgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagaccccga gatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgaacac gaatacctcg gcgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcccgagaa gaattcgaac accacggccg tctgaccgac ctgatggtaa
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa cccgcaagac gaccgcgcga
     6541 tgctgtgcgg cagccccgcg atgctgaaag atacctgcaa agttttggac gatttcggtc
     6601 tgaccgtctc tccgaaaacc ggcgtgcgcg gcgattacct gattgagcgc gcgtttgtgg
     6661 atcaataagc tgcttgaaaa tgccgtctga acggcatcag cgataaaaaa acccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg ggtaaaaatt agaaatgcac atttccgtca
     6781 ttccaacaca ggtgggaatc cggacttacc tgtttcatta tcgtttgaaa ataaaagaga
     6841 aaccaaacat atagattccc gcttgcgcgg gactgacgac ggtgttctgc gtagccaaag
     6901 cagaaaaaca tccttattgt catttcatca tgatataaca ataaggatgt taaaaattag
     6961 ttatcattat atgcatgata tataagaaaa attgtcccaa taagaaatgt tatgcagaaa
     7021 ataaaatgaa aaggatggtt ttcattgaaa atggttgttc tgccatcgtc atacacagaa
     7081 ccggtatttg gaaaaaaata atccatattg acatcattat tccatttatc gcataaatcc
     7141 actttttaat ttttttccac attaatataa tctcttattt aataaaatca aaataaaaat
     7201 caaatgccta acaattcgat ctcaaatttt gaacgccaac gtgtttgagc ggtaacaata
     7261 catactttaa gtttgcaatc cttcatcgtc ttatttttgt tttcatcaac tatacgtcta
     7321 aagcaacgcc gtccgtcatt gttcggacgg cattttttca gactgcattt tcccccgttt
     7381 gtgaaggcgc agggcgttgc ccaaaaccgc cagcgaactg aagctcatac ccagtgcggc
     7441 tatccacggt tggacatagc ccaaaacggc aagcggtacg gcaatgatat tgtacgcgcc
     7501 cgcccatatc aggttttgcc ggataatatg gcgggtgcgc cgcgcctgat cgagcaggtg
     7561 ggcgacggta cgcaaatctt cgttcaataa cacaatgtcc gcgccgtccc tcgcaatatc
     7621 cgtcccgccc gctgcggcgg cggatacgtc tgcctgcgcc aaaacgggcg cgtcgttgat
     7681 gccgtcgcct atcatcagca cttttttccc ttctttttgc aaggctttga cgtattccag
     7741 tttgtcctcg ggcatggctt gggcgcggta gtgcgcgaca cccagggcac gggcggtttc
     7801 ggcaacggcg gtttcgcggt cgccgctgag gatgtgcagg gtcaggtttt tgcctgccaa
     7861 ctgccgcacc gcctccgccg cgctgtcttt caaggggtcg gtcaggtaga acacggcttg
     7921 gaaaccgctt tgactgccga ggtaaaccgc gctgccgccg ccttctgttt gcggttcttt
     7981 acctgaaatt tcggcgacat aggatgccct gcccaatgcc caaacctgtg tttcgccgtt
     8041 gacggtcagt tgcgcgccca cgccttcgcc gatgcggttg aggcgttgtt taatagcgat
     8101 gtcggggacg ctgccgtctg aaatgcggca gttgaggatg gcgcgggcaa ggggatgttc
     8161 ggactgttgt tctaaagcct gcgccaccgc gagaacaaag gcttcgtctg tgcctctcaa
     8221 caatgagata cggcggacgg cgggtttgcc ttgggtcagc gtgccggttt tgtcgaagat
     8281 gatgtcggtg gtttgggcga gggtttcgat tgcctgcttt ccgccgatta aaataccttc
     8341 gcgcgccagc gtaccggtag aagctgccag cgcggtcggc gtggcaagcg ataaggcgca
     8401 ggggcaggta atgaccagca gggcgacggt aatccacaat gcggtgtgcg cgtcggcgta
     8461 cagcgtccag ccgatgaaga cggggacggc aagcaggagt tcgccgaata tgaaagacga
     8521 ggcgtattgt tccgccaact cggcagtgcg cggtttttgc gctaaggcgc ggtcgagcag
     8581 gcggacgatg tgcgacagtc gcgtgccgcc gccggtgcgg tcggtgcgta taatcagggg
     8641 gctttgcgtg ttgagtgtgc cggcggttac tttttcagac ggcattttgg cgacgggcag
     8701 gctctcgccg gtcagcatag attcgttgac ggcactgctt ccttccagca ccgtgccgtc
     8761 aacggggatg gtttcgcccg gtttgaccag taccacatcg cctgcttgga gcttgacgac
     8821 ggcggcttcg cggacatcct gtacggcagg atagccgggc atccggtggc agaaggcggg
     8881 tatcagtttc accagccttt cggcagcatc gcctgccttg cgccgggcaa tgtgttccat
     8941 aaagcgtccg cccagcagga aaaacagcag catggcgatg gattcgaaat acatcccctg
     9001 ccccgcattg gtcgcaaggc tgtaaacgcc ggcgataaag gtcatgatga tggcgacggc
     9061 aatcggcgta tccatgccgg cgcggaggtt tttcaggtcg cgcaacgcgc cttgataaaa
     9121 cgggacggcg caatagaata cgacgggcag caccattaaa aagccgcccc aatggaggat
     9181 ttgcaggaaa tcgggttcga tgtcgccgcc gtaaaggtag gtcggcagcg cgaacatcat
     9241 cgtctgcatc atccccagcc cggcaacggc gaggcggacg atgtattgtt ttcgttcttt
     9301 ttggttggcg gcttcgattt tttgcgcgtc atagggtgcg gcggtgtagc ctatctgcct
     9361 gattttcaac agaatgtcgg aaaggcggat tttgccgtcg tcccagacga cgcggcagcg
     9421 gtgcgtgctg taattgaggt cgatgcggac gatgccgtct gtacgcaaaa gctgctgttc
     9481 gatcagccag acgcaggcgg cgcaggtgat gccgccgagc attaaaaccg cctcgcgcgt
     9541 gccgccgtgg gtttccacaa agtcggactg gacttcgggc aggtcgtaca ggcggatttg
     9601 gtcgaggatt tcttggggcg gcagctcggt tttttgcgcg tcggcggtgc gttgtttgta
     9661 ataactgccc aagcccgcgt caataatgct ttgtgcgacc gcctgacaac cggcgcagca
     9721 ggtttcgcgg tcttcgtttt cgtaacggac agtcaggtgg aggtgttcgg gaacatccag
     9781 accgcagtgg aaacaggttt ttttcatggc atttcggttt cgtctgtgtt tggtgcggtg
     9841 gcacaatact cggcaatggc ttcgcgcagt gcgtctatac cggtattttc agcaacggaa
     9901 atgcggacgg tggcaatttt tcccgcagcg tcgcgccata tgcccgtgtt ttgttcttca
     9961 gacggcagca ggtcggtttt gttgtacacc ttgatgcacg gaatatcgcc ggcatggatt
    10021 tcttgcagta cgttttccac gtcttcaatc tgctgtccgc tgttcggggc ggcggcatcg
    10081 acgacgtgca gcagcacatc ggcttgcgcg gtttcttcca gcgtggcgga aaatgccgaa
    10141 atcagtttgt gcggcaaatc actgacgaat ccgacggtat cggtcaggat aatgctgcat
    10201 tcgggactga tgtacagccg ccgcgccgtg gtgtcgagtg tggcgaaaag ctggtctttc
    10261 gcatatatgc ccgacttggt cagccggttg aacaggctgg atttgccgac attggtatag
    10321 ccgaccagcg caaacgtttt gattgtgccc gattcgcggg gcttgcggcg cagggcgcgc
    10381 tgttttttga ggttgacaag ctgttttttc aaggcattga tccgatgggc gatcaatcgg
    10441 cggtcggttt ccagtttggt ttcgccgggg cctttcatgc cgataccgcc gcgctggctc
    10501 tgcagatggc cgtaaccgcg tatcaagcgt cccgccaaat ggctcaattg cgccaactcg
    10561 acttgcagcc tgccttcctg cgtgcgggcg cggcgggcga aaatcgccag aatcagccct
    10621 accctgtcca atacgcggca tttcagttct ttttcaaggt tgcgttcctg cgtgggcgtg
    10681 agttcgtggt tgaatacgac caaatcgatg ccgtctgcgg caactgcttc tgacagctcc
    10741 gccgccttgc ccgtgccgac aaacagcgcg gtgtgcggac ggtcgcgttt ggcagtctcc
    10801 acgcgcacgg aatcgccgcc cgccgctttg accagctcga cagcttccgc caatgccgtc
    10861 tgaaaaccgt tcagacgggt ggcactgctg cccgtaccat ctttgtccaa cattacgccc
    10921 accagcatga cgcgttcggg ttgcgcttgg gtactgccgt tgccgcctgt tctgcctgtc
    10981 atgtattcaa tcctgtaaat aaagggttac gcctcgttcc gtttctcctt ccggtgcaag
    11041 cgtgatgcgg ctgcccgcca ttccgtcaac gctgaagacg atgccgcccg cctcgtgcct
    11101 tcctatggac aggcggctgc cgggtcgctg ccggtaggta taacgggtac acgcttccaa
    11161 acaccagcgt ccgcgccagt ggatgaaata tttttcgcct tttctaaccg caagtgtctc
    11221 gccgccgttt acctcaaaat aagggtaatc gggttttcgg gttctaaact gccatttcgc
    11281 ctgcgcgcgc cgtccgttgc ggacatagtc gaataccgcc gtataaagcg tgccgtattc
    11341 caaaggcttg agcggaaaaa gcgcgaattg gtacgcggtc agcctgccgt tggggtcgtt
    11401 gccggcggtt aaaaccctga cggggcggat ttcgttttta ccctgataca gcttgaaact
    11461 tttcatcgta attttgcctg ccgcctcgga aaaatcaatg ctggcaggat tgcccgtgat
    11521 ttcatattcc ggcacggggt ctggacgctc gccgtggaaa taaggcagtg cgccgctgcc
    11581 gacgggatag gctgtataga gcagctcctg tgcgggcatg gcttcgtcgg tgtacacgac
    11641 cgcaccgtta tggcaggcgt tgcggtaata tttccgtcct gcttccggct gatttctgcc
    11701 ttgggcgcaa tgccgctcaa acctgctgtt gccctgattg aatacgagaa cggttttacc
    11761 gttttcgcgc acaaatgccg ctcctgcctc atccgtatgg cggtcaagta gggaaaggcg
    11821 gtggtagatt gcgctcatta atccgtccac ttggcgttgc tgcgtgcgga tgtcgctgtc
    11881 ggacgattcg gcggcttcct cttccgtgct gatgttttca tgcacgccgt tgtagagata
    11941 cccggcaagg cgtgtgcgtt cggtcagctt ttgtgcggtg tagtgcggat tgtcgggatg
    12001 gtgttcgccg tgtccgtctt cgggattgag cgtgagatag cgtgcgtgcc tgcgggcgga
    12061 attttccaaa accggcgcgt gtgccagctt gtgcaaaccg atttgggcgc ggatgcggtt
    12121 gaggtagtgc agcgcatcgc cgccgtccct gatttgcggt gcggacggat agacaagttc
    12181 gcccgcgggc agggattggt tttgggtatg atagaaaacg cctgccgccg aacccaacag
    12241 gagcagccga ataaaaaggg atttcataat gccgtcctcc cgacgcggca acggggtgtt
    12301 ttatcaaaac ggcaaacttg ccaatgcggt ttccgattgc agattgccaa accggcaaac
    12361 ctttccccgt gccgatgccg aacctgatgc cgtctgaacc ttcagacggc atcggatgtt
    12421 tatttgtcca cccggatggg tgcaggttca tattgtgtcg attcgtcgcc gtaatacagc
    12481 acgccgagtt tgatggggat tctgccctgc gatttgcggt gggcattgga atcgcgcaag
    12541 gaataggcgc aaccgcaata ttcctgctgg tagaagtttt cacgtttgct gatttcaatc
    12601 atgcgcgcgc tgccgccgcc tttgcgccag ttgaaatccc aatacaccac atcatcgtaa
    12661 ggcgcggcgg cgcggtgtcc gcagtcgttg atttgcgcca tatttttcca gcgtgaaatg
    12721 cccagcgaac tggtaaagac ggggaacccg tgttcatgcg cgtattgcgc cgccttttca
    12781 aaacgcatat cgaaacacat ggtgcagcgg atgccgcgtt ccggctcaaa ctccatgcct
    12841 ttggctttgg caaaccattc tttgcggtcg ttttcgtagt cgtcgtcttt atcgatgaaa
    12901 ggaatgccga acttttccgc aaagcgcacg ttttcctctt ttcggagcat atactctttg
    12961 tgcggatgga tattgggatt gtaaaaataa atggtgtagt cgatgccgga ggcaagcatc
    13021 gcctccatca cttcgccgct gcacggggcg cagcaggaat gcagcaagac ttttttatgt
    13081 ccgccgggcg ggacgagtat ggggcggtca atgtcggtaa cggtaggttt gttttgtgtt
    13141 tccattaact taacaatatc tgttgaatac ggttgaagcg gctattttat cagagccgcc
    13201 gcatgtaaaa cagagtgtca gggcatcaac ccgagctgtc tgaacgaacg gacgctttgc
    13261 gaggtaacga taaaatggtc gagcagcgac acatcgacca gcgacattgc ctgtgccagc
    13321 cgccttgtga acatgatgtc ttcctgcgaa ggttcgggcg agccgcccgg atggttgtgc
    13381 gcgataatca ggctgtcggc atattcgtcc aatgccagtt tgacgatttc gcggatgtaa
    13441 atcgtgtttt ccgcaaccgt accgcgcgac agctctctga ccgcaatcag ttggttttgg
    13501 cggttcagca gcagcgcgac gctgacttcg actttttcct gccccaaatg aaagcgtaaa
    13561 taatcggcca ccgtatccgg atcggacagg acgatgctct cctgcaattc ttccgccaat
    13621 atccgccgcc cgatttccct gaccacggca aactgtgtga aacttgccgt ccccatccct
    13681 ttgtatgccg acagtttgcc gacctccgcg ctcatcagcc tccccaaact gccgaactcc
    13741 tgcagcaaat aacgcgccaa atcaaccgca ctcattccgc gcgtgccgac gcgcagcagg
    13801 attgccaaaa gttcggcatc actcaaagcc gccgccccgc gttccaacag cttttccctg
    13861 ggcctttccc cctccggcca ttgcttgatg ctcatgtttt ccctttttta aatagtctga
    13921 gatgccgtct gaaacaaatg ttcccacacg ccaaatatcc gaaatgccgt ctgaaccgga
    13981 tttccgcttc agacggcatc tgtatttcaa aacagtctta cgccgtttct tccaactcga
    14041 ttgaagccgc cagaagcgac aggcgtgcca ttacgccgta aacgtagagg cggttgtgtt
    14101 cgttgtccac gtcgcagtct ttttcagagc gcggcatacc cagcgagtcc cattgttcga
    14161 atacgcgctt gcaggcttcg ggcgcttctt gggaattatc gccgttaccg gtaggaatgc
    14221 tgttagacag cggaacaaac accataccgc cggcgttgag gttttcgtcc gcaccgcgcc
    14281 cttcgtgtac gcggaaaaag ccgccgatga cgaagcggtc catcatatac acgacgggtt
    14341 cgcacaccgc gctgtttaag gtttcataag tataaatccc ttcttggaca atcacttcgc
    14401 tgacttccaa accttctttg actttcgcca ttttattgcg gtttttacgg ttcaatccgc
    14461 gcacttcgtc ggcggatttg acgctcatca cgcccatgcc gtaagtgccg gcatcggctt
    14521 tgacgatgac gaaaggtttg tcggtaatgc ccgattcgtc gtatttggct tgaattttcg
    14581 ccagcacacg ttctaccgcc tccgccaacg cgtcttcgcc ttcacgccct tggaagtcca
    14641 aaccgccgat tttttcaaaa taagggttaa tttgccattc gtcgatgccg attaacttgg
    14701 caaattcggc ggcaacttgg ttgtacgcgc cgaaatgatt tgttttgcgg cgcgtcgtcc
    14761 aaccgccgtg caacggcggc aaaacggttt ggctgatgcc tttgaggatg tcgggaatac
    14821 cggcggacaa atcgttgttc aacaaaacca cgcaaggcga aaagccgtct gcaagatgga
    14881 cgcggtcgcg ggtacgcaat aaaggttcca acaggatttt gtcgcccaat gcggtttcaa
    14941 attcggtcgg ttcggttact tccggattca ggctgcccaa gcgcacttca taccctgccg
    15001 aacacaaaat ctcgccgagg gcgtaaacgt tttgcaggta aaacgtattg cgcgtgtggt
    15061 tttcgggaat aatcaatacg gatttcgccg tttcacaggc gcgttgcacc gcatcttgcg
    15121 cggcaaccgc cgccagcggg ataaagttgg gattcagatt attgaagccg ccggggaaca
    15181 aattcatatc gatagacgaa attttgtaac cggcattgcg tatatcgacc gaaccgtaaa
    15241 acggcgggcg gtgtacgctc cactgcgtgc ggaaccacgc ctcgattttg gcgtgattgg
    15301 acaggatttt tgcctcaaac gcctgaagtt gcgccgaatg ttcgggcgac atcaccggta
    15361 atttcatctt tcgtctgcct ctgtgtgttt tttgacaaga tgcagatgat aatgcctttg
    15421 aaagaatgac gacaagtatt ctttcgtatc gaatcatgct ttagatataa ctgtttactg
    15481 aaatggacaa tatgtctaat tttttcgtta aaattgtttg caaaataaca ccggctcaaa
    15541 aattagacaa aatttgttgc gcggtataaa gaatacgtct aaaatccgcc caatccgcat
    15601 taccatttat ccaaaagaac aacatcatga cagcacaaac cctctacgac aaactttgga
    15661 acagccacgt cgtccgcgaa gaagaagacg gcaccgtcct gctctacatc gaccgccatt
    15721 tggtgcacga agttaccagc cctcaggcat ttgaaggctt gaaaatggcg gggcgcaagc
    15781 tgtggcgcat cgacagcgtc gtctccaccg ccgaccacaa caccccgacc ggcgattggg
    15841 acaaaggcat ccaagacccg atttccaagc tgcaagtcga tactttggac aaaaacatta
    15901 aagagtttgg cgcactcgcc tattttccgt ttatggacaa aggtcagggc atcgtacacg
    15961 ttatgggccc cgaacaaggc gcgaccctgc ccggtatgac cgtcgtctgc ggcgactcgc
    16021 acacttccac ccacggcgca ttcggcgcac tggcgcacgg catcggcact tccgaagtcg
    16081 agcacacgat ggcgacccag tgcattaccg cgaaaaaatc caaatccatg ctgattgccg
    16141 ttgacggcaa attaaaagcg ggcgttaccg ccaaagacgt ggcactttac atcatcgggc
    16201 aaatcggcac ggcgggcggt acaggctacg ccgtcgagtt tggcggcgaa gccatccgca
    16261 gcctttctat ggaaggccgc atgactttat gcaatatggc gatcgaagcc ggtgcgcgct
    16321 caggcatggt tgccgtcgac caaaccacca tcgactatgt aaaagataaa cccttcgcac
    16381 ccgaaggcga agcgtgggac aaagccgtcg agtactggcg cacgctggtg tctgacgaag
    16441 gtgcggtatt cgacaaagaa taccgtttca acgccgaaga catcgaaccg caagtcactt
    16501 ggggtacctc gcctgaaatg gttttagaca tcagcagcaa agtgccgaat cctgccgaag
    16561 aaaccgatcc ggtcaaacgc agcggtatgg aacgggccct tgaatacatg ggcttggaag
    16621 ccggtacgcc attaaacgaa atccccgtcg acatcgtatt catcggctct tgcaccaaca
    16681 gccgcatcga agacttgcgc gaagccgccg ccatcgccaa agaccgcaaa aaagccgcca
    16741 acgtacagcg cgtgttaatc gtccccggct ccggtttggt taaagaacaa gccgaaaaag
    16801 aaggcttgga caaaattttc attgaagccg gttttgaatg gcgcgaaccg ggctgttcaa
    16861 tgtgtctcgc catgaacgcc gaccgcctga ccccggggca acgctgcgcc tccacctcca
    16921 accgcaattt tgaaggccgt caaggcaacg gcggacgtac ccacctcgtc agccccgcta
    16981 tggcagccgc cgccgccgtt accggccgct ttaccgacat ccgcatgatg gcgtaagtat
    17041 tttcaggcgg tctttaagaa tttgacgcgg ccgtctgaaa tatgtcccgt aaggcagaaa
    17101 cacctttaat attcaaacca aggagtttaa ccatgaacaa acttttcatt accgccctgt
    17161 ccgccctcgc cttgtccgcc tgcgccggca cttgggaggg cgcgaaacaa gacaccgccc
    17221 gcaaccttga caaaacacag gccgccgccg aacgcgccgc cgaacaaaca ggcaacgccg
    17281 tcgaaaaagg ctgggacaaa accaaagaag ccgtcaaaaa aggcggcaat gccgtcggac
    17341 gcggcatttc ccatctcggc ggaaaaatcg aaaacgccac cgaataaccg attcgcgatg
    17401 ccgtctgaaa ccgcttcaga cggcatcgtc agcacaagac gcaaacccat gaaagccttt
    17461 accaaaatta ccgccatcgt cgccccgctc gaccgcagca acgtcgatac cgacgccatc
    17521 atccccaaac aatttctgaa atccatcaaa cgcagcggtt tcggccccaa tgcctttgac
    17581 gaatggcgtt acctcgacca cggcgaaccg ggcatggaca acagcaaacg cccgttgaac
    17641 cctgatttct cgctgaacca gccgcgttac caaggcgcgc aaatcctgtt gacgcgtaaa
    17701 aacttcggtt gcggctcttc acgcgaacac gccccttggg cattggacga ctacggcttc
    17761 cgcgccgtca tcgcccccag cttcgccgac atcttcttca acaactgcta caaaaacggc
    17821 cttttaccta tcgtgttgac cgaagaacaa gtcgatcggc ttttcaaaga agttgaagcc
    17881 aacgaaggct atcagctctc catcgacctt gccgaacaaa ccctgaccac ccctggcggc
    17941 gaaacattca cattcgacat taccgaacac cgcaaacact gcctcttaaa cggcttggac
    18001 gaaatcggac tgaccctgca acatgccgat gaaattcacg cctttgaaga aaaacgccgc
    18061 caaagccagc cttggctgtt taacggttaa aacctatgcc gtctgaaggc cgttcagacg
    18121 gcatttctga taacatataa ttgctgcaac tgatgtaaac agagggatta tcaattgctt
    18181 tcatctttca agcgggctta gcaacgctct aaattaatgc aatacaactc tatttcaaat
    18241 aaatttttag atttttaata tgcaacaaat taaatttatt gacttatttt ccggaatgag
    18301 tggcatcagg aaaggattcg aacaagcctg tcggaaacag tcggttgctt gcgaatgtgt
    18361 ttttacttca gaaatcaaac cggcagcttt ggaagtattg aagcaaaact accctgatga
    18421 agtgccgtat ggagacataa cgaaaattga aacaggggat attcccgatt ttgacattct
    18481 gttggcaggc ttcccttgtc aggctttttc tttcgccgga aaaagattgg gctttgaaga
    18541 tacacgggga acgcttttct ttgatgtggc aaggatttta aaagcaaaaa aaccaaaagg
    18601 ttttattttg gaaaatgtgg aaggattggt gacacacgat agaaaagatt cgacacaaaa
    18661 atagggcgca ccctgaccgt tattttggaa accttggaag cattgggcta ctatgtttct
    18721 tggaaagttt taaatgcaaa agatttcggc attccccaaa acaggaagcg catttatctg
    18781 acaggcagtt tgaaatccaa acccgactta tctttcgaaa caagcccaag tccgaaatta
    18841 aaaaatattt tggaatcggg actgcctact gaaagcagcc ctttcatcaa gaaattgcta
    18901 aaaaaatttc ccccgtccga actgtacgga aaatcagtaa aagacaaacg gggagggaaa
    18961 aacaatattc acagttggga tattgaatta aaaggcgcag taaccgaaga agagaagcaa
    19021 ttgttaaata tccttctaaa agaacggagg aaaaaaaatg ggcttcagaa atcggcatag
    19081 attggatgga tgggatgcct ttgacaaaag cgcaaatttc aactttctat aaacaccccg
    19141 atctccaaaa tattttggac agcctgacag ataaaggcta tttggtttta gagcatccga
    19201 aacaaaaaat tggcgggcaa agaatcaaag atgaatccct gcccaaaggc tacaatattg
    19261 tttcaggtaa aaaatctttt gaaatcaata aaatattaga tccaaacgat gttgcgccaa
    19321 ccttggttgc aatggatatg gagcaccttt tcgtcgttga caacggcggt ttgcgtacac
    19381 ttaccggaaa agaagggttg cgcttattcg gctatccgga cgattattcg tttgatattc
    19441 ccaaaaaaga cagatgcgat ttattgggta ataccgttgc cgtccctgtg attaaggcgg
    19501 tatctgaaag acttctgcat actttataaa ccgacaggga taaaatgata aaacttactg
    19561 cacaacaaat atttgataag ttgctggatg aagaaaaaat cttatcggcc aatggtcaaa
    19621 ttagattttt cttgggcgat gtggatatta tcgtcaaaca aaaagatgtt gtcggcaaca
    19681 tcattcagga atggctcggc ggatgattga gaaaaaggga aattgaattt gatgtttcaa
    19741 ccaataccca aatgcctccc gatttctttt taaataaaaa agaccgcagc agagaattgt
    19801 tggaagtaaa ggcgttcaac cgaaataccg gcccgggttt tgatattgca gattttaaaa
    19861 tgtattctga tgaaatcatt cataagccct atatgctgga tgtagactat ttaatattcg
    19921 gttacgatat ggacgacaac ggcaatgtaa ccatcaagga tttatggctt aaaaaagtat
    19981 ggcaaattac gagaagcatg gatgggtggg caattaatct tcaagtcaaa aaaggcgtgg
    20041 tgcataaaat ccgcccgggt gtttggtaca gcataaataa aaagaatatg cctatgtttg
    20101 aatgcttgga agattttgtt tccgcaattg aagaaaccgt ttatcaaaac ccggcaacac
    20161 ggcataacgc atccttatgg aaaaagaaat ttgaggaagc ctataaaaaa cactacaacc
    20221 gatcgatttc catacctcgt tggcacgaaa ttgcacacaa atacaaaaag aaataaccct
    20281 tcaaccatca acccataagg aaacttgaaa tgaccaaaca tatcgccatc ctccgaggtg
    20341 acggcatcgg tcccgaaatc gtcgccgaaa ccgtccgcgt actcgacaaa ctcatcgccc
    20401 aaggtttgga tgccggctac gaatacgccc cattgggcgg cgaagcctat gacgaatacg
    20461 gtcatcctta tcccgaattt acgcaaaacc tctgccgcaa agccgatgcc gtcctgcttg
    20521 gtgcagtcgg ttctcctcaa tacgacaacc tcgaccgtcc gttgcgccct gagcgcggcc
    20581 tgttggcaat ccgtaaggat ttgaacctgt ttgccaattt gcgtcctgct gttttatatc
    20641 ccgaattagc caacgcctcc acgctgaagc ctgaaatcgt tgccggtttg gatattttga
    20701 ttgtgcgcga actcaccggc gacatctatt tcggcgaacc gcgcggcatc cgcgttctgg
    20761 aaaacggcga acgcgaaggc tacaacacca tgaaatacag cgaaagcgaa atccgccgta
    20821 tcgcccacgt cgctttccaa tccgcccaaa aacgcagcaa aaaagtctgc tccgtcggca
    20881 aagccaacgt tttggaaacc accgaactgt ggcgcgaaat ttttgaagaa atcggcaaac
    20941 aataccctga tgtcgagctt tcccatatgt atgtggacaa tgccgctatg cagttggtac
    21001 gcgcgcccaa acaatttgac gtgattgcca ccggcaacat cttcggcgat attttatcgg
    21061 acgaggcctc tatgctgacc ggctccatcg gtatgctgcc ttctgcttct ttggacgaaa
    21121 acggcaaagg cctgtacgaa ccgtctcacg gctccgctcc cgacatcgcc ggacaaaaca
    21181 aagccaaccc gctggccacc atcctctcgc tcgcaatgtt gctgcgttac agcctgaacg
    21241 acgaagcgcg cgcgcaacaa gttgaaaacg ccgtccaaaa agtgctgcaa caaggcttgc
    21301 gtaccagcga catttacgaa gaaggcacaa aactcgtttc ctgctccgaa atgggcgatg
    21361 cggtactcgc cgccttgtaa aaaaacaaag cgcggatcac accgcgcttt ttcatctgcc
    21421 cttttccgct cgccccgttc gtcaaccgcc tgcaaagcca ccgtttaccg gcaggaaatg
    21481 ccataaatcc ccgcctatgt taggataaac gcctttactg gctcacacaa aggattatca
    21541 tcatgaaaaa aatcatcttc gccgcactcg cagcggcagc cgttggcact gcctccgccg
    21601 ccacctacaa agtggacgaa tatcacgcca acgcccgttt cgccatcgac catttcaaca
    21661 ccagcaccaa cgtcggcggt ttttacggtc tgaccggttc cgtcgagttc gaccaagcaa
    21721 aacgcgacgg taaaatcgac atcaccatcc ccgttgccaa cctgcaaagc ggttcgcaac
    21781 actttaccga ccacctgaaa tcagccgaca tcttcgatgc cgcccaatat ccggacatcc
    21841 gctttgtttc caccaaattc aacttcaacg gcaaaaaact ggtttccgtt gacggcaacc
    21901 tgaccatgca cggcaaaacc gcccccgtca aactcaaagc cgaaaaattc aactgctacc
    21961 aacgcccgat ggagaaaacc gaagtttgcg gcggcgactt cagcaccacc atcgaccgca
    22021 ccaaatgggg cgtggactac ctcgttaacg ttggtatgac caaaagcgtc cgcatcgaca
    22081 tccaaatcga ggcagccaaa caataaatct tgatgcgaat cagcacaaat gccgtctgaa
    22141 cgtccttcaa acggcatttg tgccgtcttc atcaaaccgc cccgccaacg gtaagccgat
    22201 atggcggagc acaatccgcc gaaagatatt tcagcggcag gcagggcttg ccggaaagtc
    22261 cggaaa
//
LOCUS       DAWFAD010000015        21695 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWFAD010000015 DAWFAD010000000
VERSION     DAWFAD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21695
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.015"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            54..1094
                     /gene="murB"
                     /locus_tag="WGO49_000440"
     CDS             54..1094
                     /gene="murB"
                     /locus_tag="WGO49_000440"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ0305593.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVVSAEKAATLLQRHPDMPRYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            1106..1756
                     /locus_tag="WGO49_000441"
     CDS             1106..1756
                     /locus_tag="WGO49_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ0305594.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MITKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHREKYDRKIGNGNP"
     gene            1811..2587
                     /locus_tag="WGO49_000442"
     CDS             1811..2587
                     /locus_tag="WGO49_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0305595.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGLPAPDFAPDDSMGKIIRTVCDNSLSL"
     gene            2638..3204
                     /locus_tag="WGO49_000443"
     CDS             2638..3204
                     /locus_tag="WGO49_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ0305596.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            3224..4114
                     /locus_tag="WGO49_000444"
     CDS             3224..4114
                     /locus_tag="WGO49_000444"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ0305597.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            4139..4897
                     /locus_tag="WGO49_000445"
     CDS             4139..4897
                     /locus_tag="WGO49_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0305598.1"
                     /translation="MNPKISSDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NRWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(4952..5617)
                     /locus_tag="WGO49_000446"
     CDS             complement(4952..5617)
                     /locus_tag="WGO49_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0305599.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(5730..6626)
                     /locus_tag="WGO49_000447"
     CDS             complement(5730..6626)
                     /locus_tag="WGO49_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ0305600.1"
                     /translation="MQPPKASDIISHLQIDGIFPGGNAIPCTHLNNYMNIKEKQLMNT
                     IADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVARLTMTVYLPAEQKGTHMS
                     RFVALMEQHAEALDFAQLRRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIQSLLD
                     YDVSLTGEMKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADAEVGI
                     EEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATSLIADKRIKSF
                     VVESENFESIHNHSAYAYIAYP"
     gene            6694..7851
                     /locus_tag="WGO49_000448"
     CDS             6694..7851
                     /locus_tag="WGO49_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ0305601.1"
                     /translation="MKFATQAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQVLEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNLDNLDKLLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPMDCWLVLRGIKTLALRMNAHCQNALEIARRLEAHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            complement(7914..8915)
                     /gene="hemB"
                     /locus_tag="WGO49_000449"
     CDS             complement(7914..8915)
                     /gene="hemB"
                     /locus_tag="WGO49_000449"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ0305602.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGDKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(9098..9382)
                     /gene="yhbY"
                     /locus_tag="WGO49_000450"
     CDS             complement(9098..9382)
                     /gene="yhbY"
                     /locus_tag="WGO49_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ0305603.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            9492..10112
                     /locus_tag="WGO49_000451"
     CDS             9492..10112
                     /locus_tag="WGO49_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ0305604.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFEGLLNNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            10176..12143
                     /gene="ftsH"
                     /locus_tag="WGO49_000452"
     CDS             10176..12143
                     /gene="ftsH"
                     /locus_tag="WGO49_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ0305605.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQIA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            12364..12795
                     /locus_tag="WGO49_000453"
     CDS             12364..12795
                     /locus_tag="WGO49_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ0305606.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSVTLVEAFVKEADRRYA"
     gene            12788..13066
                     /locus_tag="WGO49_000454"
     CDS             12788..13066
                     /locus_tag="WGO49_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ0305607.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            13119..13697
                     /gene="pth"
                     /locus_tag="WGO49_000455"
     CDS             13119..13697
                     /gene="pth"
                     /locus_tag="WGO49_000455"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ0305608.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPKEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            13758..13964
                     /locus_tag="WGO49_000456"
     CDS             13758..13964
                     /locus_tag="WGO49_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ0305609.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            13961..14323
                     /locus_tag="WGO49_000457"
     CDS             13961..14323
                     /locus_tag="WGO49_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ0305610.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     LTGALLALFAARSADRPD"
     gene            14554..15969
                     /locus_tag="WGO49_000458"
     CDS             14554..15969
                     /locus_tag="WGO49_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ0305611.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVALLRLSRGSMKAAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDREKERKTYVFVLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSSAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            16088..16597
                     /locus_tag="WGO49_000459"
     CDS             16088..16597
                     /locus_tag="WGO49_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0305612.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            16760..18142
                     /locus_tag="WGO49_000460"
     CDS             16760..18142
                     /locus_tag="WGO49_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ0305613.1"
                     /translation="MANIAHDIFKAYDIRGIVGKTLTDEAAYLIGKAIATKASEKGIT
                     RIALGRDGRLSGPGLMAQIQHGFTDSSIDVLNVGMIATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLAIVEKDGFVAADKQGSVTEKDISGEYHNNI
                     VGHIKLKRPMKIVIDAGNGVGGAFAGKLYKGLGNEVAELFCEVDGNFPNHHPDPSKPK
                     NLQDLIAALKNSDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLTPWIKEHGGEPVMEKTGHSFIKSAMKKTGALVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(18162..18917)
                     /locus_tag="WGO49_000461"
     CDS             complement(18162..18917)
                     /locus_tag="WGO49_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0305614.1"
                     /translation="MIKIRNIHKTFGESTILRGINLDVGKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(18927..19643)
                     /locus_tag="WGO49_000462"
     CDS             complement(18927..19643)
                     /locus_tag="WGO49_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0305615.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(19633..20460)
                     /locus_tag="WGO49_000463"
     CDS             complement(19633..20460)
                     /locus_tag="WGO49_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0305616.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            20745..21653
                     /locus_tag="WGO49_000464"
     CDS             20745..21653
                     /locus_tag="WGO49_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ0305617.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALTA
                     LKARFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT         5349 a         6385 c         5354 g         4607 t
ORIGIN      
        1 aaacgatggg ctgaagcccg ccactggcaa tactcatttt aaagttcaaa cccatgcaac
       61 ccatccgata ccgaaccgac cttaccccct acaacacttt cggcctgcgc gcccaagccc
      121 gagcctttat tgcgcttgaa cacgccgacg ggttgcgcga catcgtccgg ctacccgagt
      181 tcaaccgaga tactgtttta tggctgggcg gcggcagcaa tatccttttg atggaggact
      241 acgccggtct ggtcgtacat atggaaaaca aaggcatacg cgaaattgct cgttcagacg
      301 gcatggttct gattgaagcg caggcgggcg aaatctggca cgattttgtc ctgcacgccg
      361 tcgcgctggg tttgaacggt ttggaaaacc tgagcctgat tccgggtacg gtcggcgcgt
      421 cgcccgtgca gaacatcggc gcatacggcg tagaggcgaa agatgtgatt cacagcgtgc
      481 gctgcttcga tttggatacg gaaacctttg taaccctttc caatgccgac tgccgcttcg
      541 cctaccgcga aagcctgttc aagcaggaag gtaaagggcg ttatgtgatt gtttcggtcg
      601 tatttgcatt aaaaacgcat tttgtgccga acttgggtta cggcgatttg gcggccgccg
      661 ttgccgaact gagcgcgggc agggtgccga cggcgaaaga tgtttccgat gcagtgtgtg
      721 caatccgcaa cagtaaactt cctaatccta acgtgcttgg caatgtcggc agtttcttta
      781 aaaaccccgt cgtcagcgca gaaaaagccg ccaccttgtt gcagcggcat cctgatatgc
      841 cgcgctatcc gcagcccgac ggttcggtca aactcgccgc cggctggctg atcgaccaat
      901 gtcgtctgaa aggctttcaa atcggcggcg cggcggtaca tgacaggcag gctttggtct
      961 tagtgaacaa aaacaatgcc tcggcaaacg atgtccggca gttggcgcaa cacatcaaat
     1021 ttacagtatt tgctcggttt caggtagaat tgcacgccga acccaattgg ctgcctgctt
     1081 cgttcagcct gtaaatccaa ggagtatgcc cgtgacccgc cccgccaaaa tcaatactta
     1141 cacacgcatc atcgacgcca gccttgcgct tttcaacgag gaaggcgagc gcaacatcag
     1201 caccaaccat attgccgccc acttgggcat cagtccgggc aacctctatt accacttccg
     1261 caacaaagac gaaatcatcg tccaactgtt caaacgttac agcgaagccc tgctggaata
     1321 cctcaacgaa gccgtgttgc cgtctgatgt ggaagactcc atcaattata tggcgggcat
     1381 ttacgacgtg atgtgggaat accgcttcct attcagcgac gtgaacaccc tgcttgcacg
     1441 cagtgccgaa ctattgggcg aacacaatac cttcacccaa gccaaagtct ccccgctctt
     1501 ggtcaacctg ctgacccagc tcaacggtct gaacatcatc caagccgacc aaaccgccat
     1561 gaacgacctc gccgtcaata tgtggatgat cacgaaatac tggttcgact tcgacagctc
     1621 cctgcgcggc cgcaccaagc tgaccgaaga ctccaaagca cgcggcatcc gccgcacctt
     1681 aagcctcctt cgtccttatc ttttgcccga acaccgcgag aaatacgacc ggaaaatcgg
     1741 caacggcaac ccgtaaaccc acagcgtccg aatcagcctt cagacggcac ttacccgacg
     1801 gtatcagcga atgcgcgcag tccccccgcc ccatatctcc atcacaggcc taggttacct
     1861 cggcctgccg ctggcacaaa agttttacca acacggcagc cgcgtcgccg ccataaaacg
     1921 cagcctgact tcggacgaca tcaacctgcc catacatctc gacaccttcg acctcggcag
     1981 caccgatgcc tttcagacgg cattgtggcg gcaccacgcc gacaaacccg tctggttctt
     2041 cctgctaccg ccctcctcgc tgacgcatta cgccgacacc gtcaaacaat gggcagaact
     2101 tgcccgggcg tgcaacgtgc aacacctgat tttcacaagc agcaccagcg tttacggcga
     2161 tacagcgcgc gaatgcgacg aaaccgccac acccgatccg caaaccgagt ccgcccgcca
     2221 aatcctcgcc gccgaacaac acctgctcga cagcggcatc tccaacatcg acatcctgcg
     2281 gctgggcggg ctttattgcg ccgaacgcca tcccgtcagc cgccttgttc aaaagcaaaa
     2341 catcccgggc ggcaaccgcc ccatcaacat cgtccaccgt aatatcgccg tcgaaagcct
     2401 gtttcagacg gcatttaacc ccggcggcag gcggctgaaa aacattatcg aaccgcgcca
     2461 cccgacacga cgcgaattct acacggaaga agccgccaaa ctcggtttgc cggctcctga
     2521 tttcgcaccc gacgacagca tgggcaaaat catccgtacc gtttgcgata acagcttaag
     2581 cctgtaaaat aagtgtcaac agcaacaaac aggcattctc cgccccgatc agaaaatatg
     2641 tccgccctcc tccccatcat caaccgcctg attctgcaaa gcccggacag ccgctcggaa
     2701 cttgccgcct ttgcaggcaa aacactgacc ctgaacattg ccgggctgaa actggcggga
     2761 cgcatcacgg aagacggttt gctctcggcg ggaaacggct ttgcagacac cgaaattacc
     2821 ttccgcaaca gcgcggtaca gaaaatcctc caaggcggcg aacccggggc gggcgacatc
     2881 gggctcgaag gcgacctcat cctcggcatc gcggtactgt ccctgctcgg cagcctgcgt
     2941 tcccgcgcat cggacgaatt ggcacggatt ttcggcacgc aggcagacat cggcagccgt
     3001 gccgccgaca tcggacacgg catcaaacaa atcggcagga acatcgccga acaaatcggc
     3061 ggattttccc gcgaatccga gtccgcaaac atcggcaacg aagcccttgc cgactgcctc
     3121 gacgaaataa gcagactgcg cgacggcgtg gaacgcctca acgaacgcct cgaccggctc
     3181 gaacgcgaca tttggataga ctaaccttca gacggcatcc gacatgaaca gcccttttca
     3241 caacatcggc atcgtaaccc gccccaacac gcccgacatc caagacaccg cacacacgct
     3301 gattaccttt ttgaagcagc acggctttac cgtctatctc gacgaagtcg gcataaagga
     3361 aggctgcatc tatacccaag acaccgtcgg ctgccatatc gtcaacaaga ccgaactggg
     3421 gcaatactgc gacctggtcg ccgttttagg cggagacggc acctttctct ccgtcgcccg
     3481 cgaaatcgcc ctgcgcgccg ttccgattat cggcatcaac caagggcatt tgggcttcct
     3541 gacccaaatt ccccgcgaat atatgacgga caagctattg cccgttttag aagggaaata
     3601 ccttgccgaa gagcgcatcc tgattgaggc cgcactcatc cgcgaaggca aaaccgccga
     3661 acgcgccatc gccctcaacg atgccgtcct ctcccgtggc ggtgccggac agatgattga
     3721 gtttgaagtc ttcgtcaatc gggaattcgt ctatacccag cgttcggacg ggctgattgt
     3781 ctccaccccc accggatcga ccgcctattc gcttgccgcc ggcggcccca tcatgcaggc
     3841 aggattacac gccttcacgc tcgtccccat ctgcccgcaa tccatgacca accgccccat
     3901 cgccattcca gacacgtccg aaatcgaaat cctcgttacc caaggcggcg acgcgcgcgt
     3961 ccatttcgac ggtcaaaccc atatcgacgt gcaaaacctc gaccgcatca ccatccgccg
     4021 ctaccgaaat cccttacgca ttttgcaccc gaccgactac caatatttca aaaccctgcg
     4081 ccaaaaactg cactggggcg agcaattagt ctaagccggc ctttacccga aagtatccat
     4141 gaatcccaaa atttcctccg accatacaga agatgccgtg cgcctctcca aacgcatggc
     4201 gcaactgggg ctttgttcgc gccgcgaagc cgacggctac atcgaacagg gctgggtaac
     4261 ggtcaacggc aaaaccgccg tactcggtca gaaagtttca ccggccgacc gtatcgaact
     4321 gaacaagaaa gcccacgaac agcaggcggc acgcattacc atcctgctga acaaacccgt
     4381 cggctatgtc agcgcacaag cggaaaaagg ctataaatcc gccgccgaac tgattacccc
     4441 tgaaaaccgc tgggaaggcg acaccggccg catccgtttc gatccgaaac acaaaatcgg
     4501 cctcgccccc gccggcaggc tcgatatcga ttcagtcgga ttactggtat tgacgcagga
     4561 cggccgtatc gccaagcagc ttatcggcga aaacagcggc agtgaaaaag aatatttggt
     4621 gcgcgtgcgc ggcaaattgg acgaaaaagg acttgcctta ctgaatcacg gattgagttt
     4681 ggacggcgag aaactgcgtc ccgcccaagt agaatggcaa aacgaagacc aactgcgctt
     4741 cgtgttgaaa cagggtaaaa agcggcaaat ccgccgtatg tgcgaactgg tcggactgcg
     4801 cgtcgtcggg ctgaaacgca tccgcatggg caaggtcaaa ctcggcaggc tgccgcccgg
     4861 caaatggcgt tatcttgctc ccggcgaatc gttttaaata agcattaaaa aatgccgtct
     4921 gaaaatcctt gcgttttcag acggcattgc cttatgccca aataacggtt tcttccaagg
     4981 gcctgcgcgc cttcgtggcg atttcctgaa cgcggtaacc gaatgtcgcg gcgacggaca
     5041 cgccccattc tgccgcatcg aacaaaccga actgcccgga caatacgcgt tccatatcgg
     5101 catagttgaa accttccacc gggcaggaat cgatacctgc catcgccgca cccgtcatca
     5161 tgttgcccaa cgcgatatag gtctggcggc aacaccagtc aaacaaggcg cgcgaatcgt
     5221 ccaaaatctt gatgtcgtcg gcttgaaacg cctgatacct cgccaaagat tttgccatgg
     5281 catccggttc ggtaacgccg cgccgtttga ggctttccaa cataaacggg ctgtcgaagc
     5341 gggcattttt ctttgccaaa aacaccacca aatgactggc ggtatccaaa gcatccgcca
     5401 taccccaaga aaacggcttg attgcctgtc ggatttcagg gttttgaacc acaacaaact
     5461 gccaaggctc cgaaccgacc gaactgggcg acaaacgccc gagttctaaa ataaactgaa
     5521 aatcctcggc actgatttta cgtgccgcat cgtaatgccg gcatgatttg cggtttttaa
     5581 atgcggatag aacctgctct ctgtctaata ccgtcatagt gcatccttta ttacttttat
     5641 tgcaaattga tttcaaaccg atacggcggc agattcgcca ataacgccca aacgcctttc
     5701 agacggcatt tggttcatcg caaacacgcc tacgggtagg cgatataggc ataagccgaa
     5761 tggttgtgga tagattcgaa attctcactc tcgacgacga aactcttgat gcgtttgtcg
     5821 gcaatcagcg aagtagcgac atcgcgcacc atatcttcca cgaatttcgg gttttcgtag
     5881 gccttttcgg taacgtattt ttcatcgggg cgtttgagca ggccgtagag ttggcagctc
     5941 gcctgcgcct ccacataatc gatgacttcc tcgataccga cttcggcatc ggcagtcagg
     6001 ctgacggtaa cgtgcgaacg ctggttgtgc gcgccgtatt gggaaatttc tttggaacac
     6061 gggcaaagcg aggttacggg aatcatgacc ttcatactgt ggccgtatgc accgtctttc
     6121 atttcgcccg tgaggctgac atcataatcc agtaaagact gaataccgga aaccggggcg
     6181 gttttcttgc ggaaaaatgg gaaagaaacg ctgattttgc cggcgcggga atccaaaagc
     6241 gcgaccattt cagcagtcag cctgcgcaat tgtgcaaaat ccaaggcttc ggcatgttgc
     6301 tccatcaatg cgacaaagcg cgacatatgc gtacccttct gctcggcggg caggtaaacc
     6361 gtcatggtca gacgggcaac tgtggactgg atgccttctg cagtttgcag ggtaatcgga
     6421 aagcgcaggt ctttgatacc gacctgattg atcggcagat tgcgcaaatc tcggctggat
     6481 tgcacgtctg caatagtgtt catgagttgt ttttccttaa tattcatgta attgttgaga
     6541 tgtgtgcatg gtatggcgtt tccgccgggg aatataccgt caatctgcaa atgcgagatt
     6601 atatcacttg cttttggcgg ctgcattgat tgtttctatt ttcgggatag tgataaaatc
     6661 ccgacttatt catatcttac aaggcagaaa cagatgaaat tcgccaccca agccatccat
     6721 tccggctacg attgcgacga acacaaccgc gcgctgatgc cgccgattta tcaaaacagt
     6781 atgtttgcgc tgcacgaaat cggcgaaaat gtgccttacc gctattcacg tctgagcaac
     6841 ccgacccgtc aggtattgga agataccgtt gccgatttgg aacacggtgc ggcaggtttt
     6901 gcgttttcca gcggtatggc agggattgat gccgtatggc gcaccttcct gcaaccgggc
     6961 gataccattg tcgccgtcgc cgatatttac ggcggcgcgt atgatttatt ggtcgatgtt
     7021 tatcaaaaat ggggggtgaa cgttgttttt gccgatttgg gcaatctgga taatttggac
     7081 aagctgctta aagcgcacaa ggtcaaactg gtttggctgg aaacgccgtc caatccgctt
     7141 ttgcgcttgg tagacatcaa agcgcttgcc gcgaaagcca aagcggccgg cgcactggtc
     7201 ggcatcgaca acacctttgc cacgccgtat ctgcaacagc ccttggatat gggttgcgat
     7261 tttgtgttcc attccgctac caaatatttg tgcggccatt ccgacgtgtt gatgggcatc
     7321 gtcgttgcca aaaccaaaga gctggcgcag cctttgcacg atatgatggt gcataccggc
     7381 gcggttgccg gcccgatgga ctgctggctg gtgttgcgcg gcatcaaaac actggctctg
     7441 cgcatgaacg cccattgcca aaacgcactc gaaatcgcgc gccgtttgga agcccatcct
     7501 gccattgaaa aagtgttcca tcccggcctg ccgtctcacg aacattatga actcgcccaa
     7561 gcgcaaatgc ctaaaggcat cggcggcgtg gtgacggttt atctcaaaaa cgacacgcgc
     7621 gaagcggcaa acagcgtgat taaaaacatg aaactggtca aaatggcttc cagcctcggc
     7681 ggcgtggaaa gtttggtcaa ccattgctat tcccagtccc acagcggcgt gccgcataat
     7741 gtgaaaatgg aaatgggcat caaagtcggc ctgctgcgtt tctccatcgg catcgaagac
     7801 gcggacgata tttggaacga tatttccgcc gcactcgata caactttgta aaccgtaaaa
     7861 atgccgtctg aaaccatagt ttcagacagc atttcaatat tgcgaatcaa atatcaacgc
     7921 ttcagcatct tggccgcctc aatggcataa taggtcaaaa tcccatccgc gcccgcacgt
     7981 ttgaatgcca gcaggctttc caaaaccact ttatcgccgt ccagccatcc gttggcaacc
     8041 gccgcctgca gcatcgcata ttcgcccgaa acctgatagg cataagtcgg cacaccgaac
     8101 tcgtccttca cgcggcggac aacgtccaaa tacggcaaac cgggcttcac catcaccata
     8161 tccgcacctt cctgaatatc gagcgctact tcatgcagcg cctcatcggt gtttgcggga
     8221 tccatctgat aggtcttttt atctgccttg cccaaattgc ccgaactgcc taccgcatca
     8281 cggaaagggc catagaatgc agaagcatat ttggcggaat atgccataat ccgcgtgtgg
     8341 atatgtccgg catcctccaa cgcctcgcga atcgcaccga tacgcccgtc catcatatcc
     8401 gacggagcaa ccacctgcgc gcccgcttca gcgtggcaca aagcctgctt gaccaaaacc
     8461 tctacggttt catcgttcat cacataaccg ttttcgtccg tcagcccgtc ctgaccgtga
     8521 accgtataag gatcgagcgc gacatccgtc ataatgccca gttcgggaaa cctctcgcgc
     8581 aaggcgcgga cagttgacgg cacgagtcct tcgggattgt acgcctcctg cgcacgctcg
     8641 gttttgtttg ccgtaaccac ggggaacagt gccaacatcg gaataccgag ctttaccgcc
     8701 tcttccgccg taaacagcag cctgtccaaa ctttgacgct tcacacccgg catagaaggc
     8761 acatcctcct cgcgcgccga cccctccaat acgaacaccg gataaatcaa atcatcggcg
     8821 gtcagcgtgt gttcgcgcat caggcggcgt gaaaaatcgt ccctgcgcat acggcgcata
     8881 cgcgaagccg gaacattgcg gtaaggaaac tgcataagcc ctccaatcat cctgtcaaca
     8941 attcaaacca tacggaagcc gccgcaactt gcagacagca tcgggcggca atcaaaatcc
     9001 gttttcaccg gaaagggttt cggcccgccg aaatgccgcg ccgaaggacg gcgtcccgcc
     9061 cattaaagaa caatagcaaa acaacagctt caggctgtca gccttcgata ttcttacgcc
     9121 acaataccaa aagtttcccg atatgctgaa caggttgcgc gtcaaccgcc tcacataagg
     9181 cagtgcagat ttcgatacgt tcggcacggt cgtcgccgaa tacgcgcact ttaatcagct
     9241 catgcgccgt caatgccgca tcggtttcct tgatgaccgc gtccgtcaga ccctgctgac
     9301 cgaccatcac aacaggatgg agatggtgcg cgcgcgcttt cagttccaaa atttctttgg
     9361 tgttcaattt ggtatccgtc attttcctac tgcttgaata aaaagaataa tgcggcattg
     9421 tacgcgattt ggcacgacat cggcaaaatt aacgtacaat acgcggtttc cattccgacc
     9481 cgcgaaccac tatggctgta cgttccaaat cctcaaaagc gtggctgcac gaacacgtca
     9541 acgaccacta cgtccatatg gcgcaaaaag acggctaccg cgcccgtgcc gcatacaaac
     9601 ttttggaaat caacgaaaaa gacaaattaa tcaaacccgg cacggtactt gccgacttgg
     9661 gcagcgcacc gggaagctgg tcgcaggttg ccgccaagct gacgggtact tccggagcag
     9721 ttttcgcctt ggacatcctg cctatggaag ccataggggg cgtctccttc attcagggcg
     9781 acttcaggga aaacgacgtt ttggcgcaat ttgaaggctt attaaacaac cgccccttag
     9841 accttgtaat ctgcgatatg gcgcccaata tgtcgggaaa cgccgtaagc gatcaggcgc
     9901 gcagctttta tttgtgcgaa ctggctttgg acttcgcctc gcaacacctg aaaaccggcg
     9961 gcagcttttt ggtcaaagtc tttcagggtg caggctatca ggaatacatg gcagccatgc
    10021 gcgaaatttt cggcacggtg cagacgcgca aacccgaagc ctcgcgcaat cgctccagtg
    10081 agatttattt attgggcaaa aataaacgct gacaatacag acggcgtgct ttacaatcat
    10141 ttccgtttta cacctcaatt atggagcctt gctaagtggg gaacaccttt aaatcaatcc
    10201 ttgtctgggt cgccttgggt atcggcctga tggctgcgtt caacgcttta gacggtaaaa
    10261 aagaagacaa cgggcaaatc gaatattctc agttcatcca acaggtcaac aacggcgaag
    10321 tatccggcgt caacatcgaa ggatccgtcg tcagcggcta cctgattaag ggcgagcgca
    10381 ccgacaaaag caccttcttc accaacgcgc ctttggacga caacctgatt aaaacactgc
    10441 tcgacaaaaa cgtccgtgta aaagtaacgc cggaagaaaa accgagcgcg ctggctgccc
    10501 tgttttacag cctgctgccc gtcctgctgc tgattggcgc gtggttctac tttatgcgta
    10561 tgcagacggg cggcggcgga aaaggcggcg cattctcatt cggcaaaagc cgcgcccgcc
    10621 tactggacaa agatgccaac aaagtgacct ttgccgatgt cgccggctgc gacgaagcca
    10681 aagaagaagt gcaggaaatc gtcgattacc tcaaagcgcc gaaccgctat caaagcctgg
    10741 gcgggcgcgt gccgcgcggc atcctgctgg cgggcagccc gggtacgggt aagacgcttt
    10801 tggcgaaagc gattgcaggc gaagccggcg tgccgttctt cagcatttca ggttccgact
    10861 ttgtcgaaat gttcgtcggt gtcggtgcaa gccgcgtccg cgatatgttc gagcaggcga
    10921 agaaaaacgc cccctgcatc atctttatcg acgagattga cgcagtcggc cgccaacgcg
    10981 gcgcaggttt gggcggcggc aatgatgagc gcgagcaaac attaaaccaa ttgttggttg
    11041 aaatggacgg ttttgagagc aatcagactg taattgtgat tgcggcaacc aaccgccccg
    11101 acgtactcga tcctgcgctg caacgccccg gccgtttcga ccgccaagtc gtcgtccccc
    11161 tgccggacat ccgggggcgc gaacagattt tgaacgtcca ttctaaaaaa gtgcctttgg
    11221 acgaatctgt ggatttattg tccctcgcgc gcggcacgcc gggtttttcc ggtgcggatt
    11281 tggcaaacct ggttaacgaa gccgccctgt ttgccggccg ccgcaacaaa gtcaaagtcg
    11341 atcaaagcga ttttgaagac gcaaaagaca aaatctatat gggtccggaa cgccgcagta
    11401 tggtgatgca cgaagacgaa aaacgtgcga cggcgtatca tgagtccgga cacgcgattg
    11461 ttgccgaaag cctgcccttt accgaccccg tccacaaagt aaccattatg ccgcgcggac
    11521 gtgcgctggg tctgacttgg cagcttccgg agcgcgaccg catcagtatg tataaagatc
    11581 agatgttgag ccagctctcc atcctgttcg gcggacggat tgccgaagac atcttcgtcg
    11641 gacgcatctc caccggcgca tcaaacgact ttgaacgcgc aacccaaatg gcgcgcgaaa
    11701 tggtaacgcg ctacggcatg agcgacaaaa tgggcgtgat ggtttatgcg gaaaacgaag
    11761 gcgaagtctt cttaggacgc agcgtaaccc gttcgcaaaa catttccgag aaaacccagc
    11821 aggacatcga tgcggaaatc cgccggattt tggacgagca gtatcagatt gcctacaaaa
    11881 tcctcgatga aaaccgcgac aagatggaaa ccatgtgcaa agccctgatg gaatgggaaa
    11941 ccatcgaccg cgatcaggta ctggaaatca tggcgggcaa acaacccagt ccgcccaagg
    12001 attacagcca caacctgcgc gagaatgcgg acgcggcgga agataacgcg ccgcacgctc
    12061 cgactcggga agaaaccgaa gcacctgccc cggcagacac cgcttcgaca gagtccgagc
    12121 agcagcctga aaacaaggct taacttcccg aacaaacggc agcccgcaag ctgccgtttt
    12181 cccatcctgt tttcagacgg cattttccgc tcaatgccgt ctgaaacgcc aaagccgtat
    12241 cggtttttgc ggatctgcga tatggcggtt taaattgaaa tcagacgagg caaggctgta
    12301 ccggtttaaa attaaaccgc tataataccg cccttcgaat acacgcccgc attggtgatg
    12361 cctgtgaaaa aagttgaaaa aaacatcttg gtgctgcacg gcgcggacaa aatgttcgag
    12421 ctggtcgata aggttgaaga ctatccgcac tttctgccgt ggtacagcaa gaccgaggtc
    12481 atcgggcgta gcggcaacga actgaaggcg cggctgttta tggactatat gcacgttcgc
    12541 caatcattcg ccacgcacaa ccgcaacatt ccgggcaggg aaatccgtat ggaactgctc
    12601 gagggtccgt tcaaaacctt acgtggaacg tggaaattta tcgatttggg cgacgatatg
    12661 tgcaaaatcg aattcaattt ggaatacgat ttttccaatg ccgttttgtc cgccttaatt
    12721 tcccccgtct tcaaccacct ttccgtcacg ctggtcgaag cgttcgtcaa agaggcagac
    12781 cgccgttatg cttgaaattg agattgtgta cgggctgccc gaccgacagg ttttgaaaac
    12841 catgcagctt gccgaaggaa caaccgtccg cgccgccgca ctgcaaagcg gtttggacgg
    12901 catatttgaa gatttaaacc tgcattccgc gcctttgggc attttcggca aagccgtcaa
    12961 agacgacacg ccgctgcgcg acggcgaccg catcgaagtg taccgcccgc tgttgatcga
    13021 ccccaaagaa gcgcgccgca aacgcgttca aaatcaagaa gaataaccat gccgtctgaa
    13081 gccttcagac agcattcgac agaaacacgg aaaatatcat gtcaaacaca atcaaaatgg
    13141 ttgtcggctt gggcaacccg ggcaaagaat acgaacagac acgccacaat gcgggttttt
    13201 ggttcctcga cgaactggcg tggaaatgga aggcttcatt taaagaagaa aaaaaattct
    13261 tcggcgaagt cgcccgtgcc gccctgcccg acggcgacgt ttggctgctc aaacctgcca
    13321 cgttcatgaa ccgttccgga caggcagttg ccgcccttgc gcagttttat aaaatcaaac
    13381 ccaaagaaat cctcgtcgtc cacgacgaac tcgacattcc ctgcggacgg atcaaattca
    13441 aactcggcgg cggcaacggc ggacacaacg gcttgaaaga cattcaggca aaactcggca
    13501 cggcagacta ttaccgtctg cgcctcggca tcggccaccc gggcgaccgc aacctcgtcg
    13561 tcggctatgt cctgaacaaa cccagtacgg aacaccgccg acagattgac gatgccgtcg
    13621 ccaaatccct gcaggccata cccgacatcc ttgccggcaa atgggaagag gcaacccgct
    13681 tcctgcacag caaatgaccc gatgccgtct gaagcccttt cagacggcat gttcccgatt
    13741 tccatatccg aacagtcatg accgaactca agcagcttat ccaaaccgaa tccatccccg
    13801 tcatcgaaga aaccctcgat ttcctgctgt acgaatgcag catcgacgac gcaccatccg
    13861 ccgaagaagt ggcacaatgg cgcgacatac ttgccgcacg cggcggcaaa ttcctgcgcc
    13921 tgtccaaaat ctgccaaacg tggctggacg aggaggcggc atgaagctgc cgcgcaaccg
    13981 cttcagcctg ctttccgcat tgtggtttgc cggcggcatc tattcgctgc tcttcaaagc
    14041 tgccgacacc gcgccgccgc cgtttccgca tttcgacaaa gcagcacacc ttgccctgtt
    14101 tttcgcacaa atctggcttt tgaccaaagc attcaaaacc ggaaaacttc ccatccccta
    14161 ccgcagcctg atggtctttg ccctctgttt cgccctcttc agcgaatgcg cgcaggcatg
    14221 gtttaccgca acgagaaccg gcagtttggg cgatgtcctt gccgacctga cgggtgcatt
    14281 gctcgcactc tttgccgcac gttccgccga ccgcccggac tgaatcggtt tattttcccc
    14341 aaacaggatg cacctcttcc cgatacatcc tatttttccc cttacatatc aatctcatct
    14401 cataaacaaa accgacaaat cgtttacaat atatttacac tacatcagat tacaaatata
    14461 ctcgaaccag ttgcagaagc ggcgattcca caaaccgttt cggattgcac gacacaaaag
    14521 aaaaaccgat tttgttgcct taaaggagta ttcatgaacc tgcatgcaaa ggacaaaacc
    14581 cagcatcccg aaaacgtcga gctgctcagt gcgcagaagc cgattaccga ctttaagggc
    14641 ctgctgacca ccattatttc cgccgtcgtc tgtttcggca tttaccacat cctgccttac
    14701 agccccgatg ccaataaagg tatcgcgttg ctgattttcg ttgccgcact ttggtttacc
    14761 gaagccgtcc acattaccgt aaccgcactg atggtgccga ttctcgccgt cgtactcggt
    14821 ttccccgaca tggacatcaa aaaggcgatg gctgattttt ccaacccgat tatctacatt
    14881 tttttcggcg gcttcgcgct tgccaccgcc ctgcatatgc agcggctgga ccgtaaaatc
    14941 gccgtcgccc tgctgcggct gtcgcgcggc agtatgaagg ccgccgtgct gatgttgttc
    15001 ctcgttaccg cctttctgtc catgtggatc agcaacaccg ccaccgccgc gatgatgctg
    15061 cctctggcaa tgggtatgct gagccacctc gaccgggaaa aagaacgcaa aacctacgtc
    15121 ttcgtcctgc tcggcatcgc ctattgcgcc agcatcggcg gcttgggcac gctcgtcggc
    15181 tcgccgccca acctgattgc cgccaaagcc ctaaatctgg acttcgtcgg ctggatgaag
    15241 ctcggcctgc cgatgatgct gttgattctg cccttgatgc tgctctccct gtacgtcatc
    15301 ctcaaaccta atttgaacga acgcgtggaa atcaaagccg aatccatccc ttggacgctg
    15361 caccgcgtga tcgcgctgtt gattttcctt gccacagccg ccgcgtggat attcagctcc
    15421 aaaatcaaaa ccgccttcgg catttccaat cccgacaccg ttatcgccct gagtgccgcc
    15481 gtcgccgtcg tcgtcttcgg cgtggcgcaa tggaaggaag tcgcccgcaa taccgactgg
    15541 ggcgtgttga tgctcttcgg cggcggcatc agcctgagca cgctgttgaa aacatccggc
    15601 gcgtccgaag ccttgggaca gcaggttgcc gccacctttt ccagcgcgcc cgcatttttg
    15661 gtgatactca tcgtcgccgc cttcattatt tttctgaccg agttcaccag caacaccgcc
    15721 tccgccgcat tgcttgtacc gattttctcc ggcatcgcta tgcagatggg gctgcccgaa
    15781 caagtcttgg tattcgtcat cggcatcggc gcatcttgtg ccttcatgct gccggttgcc
    15841 acaccgccta acgcgattgt gttcggcacg ggcttaatca agcaacgcga aatgatgaat
    15901 gtcggcatac tgctgaacat cctctgcgta gtattggttg ctctgtgggc ttatgctgta
    15961 ctgatgtaaa ccatcgacct aaacaacaag accgtctgaa agaatatttt tcagacggcc
    16021 ttgaagtttg tcgttcagac acaatttgtc gaatcattca aaaccggatt ctaacgaaag
    16081 gaaacccatg attatcctgc acaccaacaa aggcgacatc aaaatcgaac tcgatttcga
    16141 caaagccccc gttaccgcca aaaacttcga gcaatacgtc aaagacggct tctacgacgg
    16201 cgtaatcttc caccgcgtca tcaaaggctt catgattcaa ggcggcggca tggatgaaaa
    16261 catgaacgaa aaagaaaccc gcgatccgat tcaaaacgaa gcgtccaacg gcctgcccaa
    16321 cgataaatac accatcgcca tggcacgcac ttccgacccc cattccgcca gcgcgcaatt
    16381 cttcatcaac actgccgaca acgctttcct gaacttccgt tctaaagagc tgtacggcaa
    16441 aaccgtcgtc caagactggg gctatgccgt attcggtaaa gtcgttgacg gttttgacgt
    16501 tgtcgatgcc atcgaaggcg tatccaccaa acgccacggc taccatgacg acgtaccgag
    16561 cgaacctgtc gtcatcatta aagccgaagc ggtataaacc ggcaatccga aagcagcctg
    16621 cacaaaggct gctttttttg tttcaggcgg cataaaatcc aagcacatat gccgtctgaa
    16681 cacctcccgc aagccttcgg acaatgttgc aacgtgttaa aatagcctcc tttcctgaaa
    16741 ccgaaacgag ggaaccccta tggcaaatat cgcccacgac attttcaaag cctacgacat
    16801 ccgaggcatc gtcggtaaaa ccctgaccga cgaagctgcc tatttaatcg gcaaagccat
    16861 tgccaccaaa gcctccgaaa aaggcattac ccgcatcgcc ctcggccgcg acggccgttt
    16921 gagcggcccc ggcctgatgg cacaaattca acacggtttt acagacagca gcatcgacgt
    16981 cctcaacgtc ggcatgattg ccactcctat gctctacttc gcagccatca acgaatgcgg
    17041 cggcagcggc gtgatgatta ccggcagcca caatccgccc gattacaacg gtttcaaaat
    17101 gatgctcggc ggcgacacgc tcgcaggcga agccattcaa gaacttttag ctattgttga
    17161 gaaagacggt tttgttgccg ccgacaaaca aggcagcgta accgaaaaag acatctccgg
    17221 cgaataccac aacaacatcg tcggccacat taagctcaaa cgtccgatga aaatcgtgat
    17281 tgacgcgggc aacggtgtcg gcggcgcgtt tgccggcaaa ctctacaaag gtttgggcaa
    17341 tgaagtggcc gaacttttct gcgaagtgga cggcaatttt cctaatcacc accctgatcc
    17401 ttccaaaccc aaaaacctgc aagatttgat tgccgcgctg aaaaacagcg atgccgaaat
    17461 cggcttggct tttgacggcg atgccgaccg cttgggcgtg gtcaccaaag acggcaacat
    17521 tatttatccc gaccgccaat taatgctgtt cgcccaagac gtgttgagcc gcaatcctaa
    17581 agcaaaagtg attttcgatg tcaaatccac ccgcctgctg accccgtgga ttaaagaaca
    17641 cggcggcgaa cctgtgatgg aaaaaaccgg ccacagcttt atcaaatccg ccatgaaaaa
    17701 aaccggcgcg ctggttgccg gcgaaatgag cggacacatc ttcttcaaag aacgctggtt
    17761 cggcttcgac gacggtctgt acgccggcgc acgcctcttg gaaatcctgt ctgcctccga
    17821 taatccgtcc gaagtgttaa acaacctgcc gcaaagcatt tccacgcccg aactcaacat
    17881 cgccctgccc gaaggcagca acggccatca ggttatcgac gaactcgccg ccaaagccga
    17941 atttgaaggc gcaaccgaaa tcatcaccat cgacggtctg cgcgttgaat ttcccgacgg
    18001 cttcggtctg atgcgtgctt ccaataccac gccgattctg gtgttgcgtt ttgaggcgga
    18061 tacgcaagaa gccatcgagc ggattcaaaa ccaattcaaa gccgtcatcg aaagcaatcc
    18121 gaatctaatc tggcctctgt aaacacagga aaaatgctta atcaaatctt ggtagattgg
    18181 atttggctta aaaatctccg cgtccgttcg tgtttggggt ggtcgaacaa ctctttcggg
    18241 ctgccctgct ctacgataac gccgccgtcc atcacgacaa cggtcgtggc aacttccagc
    18301 gcgaacttga tttcgtgggt aacgacgacc atcgtccaac cttcccgcgc caattccttc
    18361 atggcgttca acacgtcttg caccaattca gggtcgagtg cggaagtggg ttcgtcaaac
    18421 aacatcagct cgggctgaat cgccaatgcg cgggcaatgc cgacgcgctg ctgctgaccg
    18481 ccggaaagct ggtagggata caaatccact ttgtcgccca agccgacttt ttccagcagt
    18541 ttcagagcct cttcgcgcgc ttgggcggca ggcttgccct gtacggcaac cggtccttcc
    18601 attacgtttt ccaaggcggt tttgtgcgga aagaggttgt attgttgaaa caccatgcct
    18661 gatttgcggc gcagtgccaa aatatcgtgt ttgcttggtt ttttagaaaa atcgattttc
    18721 agcggtcgct cgttgtcgaa ctcgatttgt ccgtcttcgg gcatttccaa cgcgtttagg
    18781 catcgcagaa acgtcgtttt gcctgagccg gaaggcccga ggataacgac cacctgccct
    18841 ttgcccacat ccaaattgat gccgcgcaaa atagtgcttt cgccgaaggt cttatggata
    18901 ttgcggattt taatcataac aactccttat ttggcgacat aacggtcgaa acgtttttcc
    18961 aaacgtgcct gaatcaggaa cagaaccttg caaaaacacc agtaaaccaa agcggcttcg
    19021 atatagacgg gcaaaaagtc ataagtgcgg tttgccgttt cctgcgcgac gcggaataat
    19081 tccgttaccg tcacgactgc cgcgagcgag gtgtttttaa acaaaccgat aaactcgttg
    19141 ctcaaaggcg gcacggcaac gcgaaatgcc tgcggcgcga cgatgcggcg gaacgtctgc
    19201 atataggtca taccgatgga gaaacctgct tcccattggc ctttaggtac ggacaaaatt
    19261 gccgcgcgta tggtttcgga agcgtatgca ccgacattga gcgagaagcc gataatggcg
    19321 gcgggaatcg gatcgatata gataccgacg gaaggcagcc cgtaaaacac aatcacaagc
    19381 tgaaccaaca gcggcgtacc gcgaatgacg gaaatataaa attccaccaa tttcagcagg
    19441 attttccgca cgatgccgcc ggcgggcata atccgcacca aagccacggc taccgcaatc
    19501 atcataccga taacgaaaga agctgccgcc aaaggcagag agaccgcgaa gccggctttg
    19561 accataggca aaaacgcgct gacaatcata tcggcgcgtg tttccgtcat aaacggcagc
    19621 gaagcaagga aattattgaa cactgatgtc ttttccgaag aattgttcgc ccagtttttt
    19681 cagcgtaccg tcggctttca gctcgttgat tgccgtactg aatttcgcca cggcttcgtc
    19741 attgcccttg ttgacaatca ggccggaacc gactttttca tcggcaggtg cggaccaaac
    19801 gattttcacg cccgcattcg ggtttttctt cagatagtcc aaaaccgcca attcgtcgtt
    19861 cagggttgca tcggcacgtt tttgttcaat cagggtcagc gattgcgcca aaccgtccac
    19921 tgccaccaaa tctgcgcctg cagctttggc tttttcgccg tagttgctgg tcagggattg
    19981 tgcggttttc acgcctttga tgtcgtcgat agatttgatg ttgctgtcgt tacgggcaac
    20041 caatacggca ccgctccagc tgtaaggatc ggatttgtcg aatgtcgctt ggcgttcggg
    20101 gctggtcaga ccgacttggt ttgccaccac gtcgaaacgc cccgccttca aacccgccat
    20161 catcgaatcc cattgcgttt ctttaaactc gactttcacg cccagttttt ccgccacggc
    20221 gcgggttact tccacatcgt aaccggtcag tttgccgtcc ttgtcgtggt aggtaaacgg
    20281 tgcgtaagtg ccttccgtgc cgacggtaac cgtgccttta ttgttgatgc gctcgattaa
    20341 agaaccggaa actgccgatt gtgcaggcgc ggaagatgct ccgctgccgc tttccgaacc
    20401 gccgcaggcc gccaaaacca atgcggcaat gccgccgagt acgaattttt tcaacatcat
    20461 tctctcctgt aaaaagtgtg caagtgcggc attctaagtg aaattgcggc aaacacaaag
    20521 aatacttttt ttatatggtt attccaaatc ggaatggaaa agatatgccg aaaccgccgg
    20581 aagcggaaat aaagggggcg ggaaaggcga cgggcggcac gaaaccgaaa agcggcatca
    20641 ggtatgcaaa cggcggattc acgcacgccg ttccggcaaa aatccgcctg ccgattcggt
    20701 gctataatcc atccgaaaca gatgacacca aaaaggaaca tctcatgaac acgccccaat
    20761 ccgccatcat tcccgaccac gcccaagccg gcatctttat cgaagccgac ttcgccgcca
    20821 accgcctcaa cgatattaaa gccgcctgcc gcgcttcgct cgacgcgtta actgccttga
    20881 aagcccgctt tccggacgat attttgggtc tgaccatcgc cttcggcagc aaagcctggg
    20941 caacattcgg acatacggac gaaggcagtg aaatcaaacc cttctccgaa atgggcaacg
    21001 ggcttgcgcc gtccacgcag cacgatatgt ccatccacat ccaatccttc cgccaaaacg
    21061 ccgcctacgc gcttgcccaa tccgttttag gcgcgttcgg cgacagcata tgcgtcgcgt
    21121 ccgaagaaca cggtttgcgc ctgtatcagg atcgcggact ggacggtttc gtcgacggca
    21181 cggaaaaccc gcagggcgat gaaaatgtcc gcgaagtcgc catcatcccc gaaggaaaac
    21241 ccgatgcggg cggcagctat gtcctgctgc aaaaatacct gcacgatttg aaaaaatggg
    21301 atgccgtccc cgtcgccgaa caggaggcct cggtcggacg cggcaaggaa accgacgacg
    21361 aattcggccg cgatgtccgc ctgcccgatt cgcacctcgg ccgcgtcaac ctgaaagaaa
    21421 acggagtcgg cctgaaaatc gtccgccgca gcctgccctt cggcaaaatc agcggcgaac
    21481 acggtttgat gtttaccgca tactgccgca cgctgcacaa tatcgaagcg caattattga
    21541 gtatgttcgg cgatacggac ggcaaaaccg acctgctgct ccgacacctc tccgccgccg
    21601 tttcgggcgg atactactac gcgccgtctg tcgaacgcct gcaaaacctc taagccggac
    21661 gatgccgtct gaaaccattt ttcagacggc atttt
//
LOCUS       DAWFAD010000016        21669 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWFAD010000016 DAWFAD010000000
VERSION     DAWFAD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21669
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.016"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(45..1286)
                     /gene="odhB"
                     /locus_tag="WGO49_000465"
     CDS             complement(45..1286)
                     /gene="odhB"
                     /locus_tag="WGO49_000465"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ0305618.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAATA
                     AAEAPAAAPAEAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDV
                     QNAAAKPAAAAAPAVALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNM
                     KPIMDLRAKYKEKFEKEHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFD
                     IGIAIGSPRGLVVPILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNG
                     GTFGSMMSTPIINPPQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREA
                     VLTLVAIKDALEDPARLLLDL"
     gene            complement(1386..4214)
                     /locus_tag="WGO49_000466"
     CDS             complement(1386..4214)
                     /locus_tag="WGO49_000466"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ0305619.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVALCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4419..5702)
                     /gene="gltA"
                     /locus_tag="WGO49_000467"
     CDS             complement(4419..5702)
                     /gene="gltA"
                     /locus_tag="WGO49_000467"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0305620.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPAGER"
     gene            complement(5814..6062)
                     /locus_tag="WGO49_000468"
     CDS             complement(5814..6062)
                     /locus_tag="WGO49_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ0305621.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6066..6773)
                     /locus_tag="WGO49_000469"
     CDS             complement(6066..6773)
                     /locus_tag="WGO49_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ0305622.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRVV"
     gene            complement(6894..8657)
                     /gene="sdhA"
                     /locus_tag="WGO49_000470"
     CDS             complement(6894..8657)
                     /gene="sdhA"
                     /locus_tag="WGO49_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ0305623.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDSQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8660..9001)
                     /gene="sdhD"
                     /locus_tag="WGO49_000471"
     CDS             complement(8660..9001)
                     /gene="sdhD"
                     /locus_tag="WGO49_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ0305624.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8995..9372)
                     /gene="sdhC"
                     /locus_tag="WGO49_000472"
     CDS             complement(8995..9372)
                     /gene="sdhC"
                     /locus_tag="WGO49_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ0305625.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9651..11054)
                     /locus_tag="WGO49_000473"
     CDS             complement(9651..11054)
                     /locus_tag="WGO49_000473"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0305626.1"
                     /translation="MKKIQADVVVIGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11303..12040)
                     /locus_tag="WGO49_000474"
     CDS             complement(11303..12040)
                     /locus_tag="WGO49_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ0305627.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12319..14595)
                     /gene="metE"
                     /locus_tag="WGO49_000475"
     CDS             complement(12319..14595)
                     /gene="metE"
                     /locus_tag="WGO49_000475"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ0305628.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGTVEFNRLSLLQKLLPVYVEILNALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGIIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            complement(14733..15611)
                     /gene="metF"
                     /locus_tag="WGO49_000476"
     CDS             complement(14733..15611)
                     /gene="metF"
                     /locus_tag="WGO49_000476"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ0305629.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDELPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15811..16086
                     /locus_tag="WGO49_000477"
     CDS             15811..16086
                     /locus_tag="WGO49_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ0305630.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16086..16211
                     /gene="ykgO"
                     /locus_tag="WGO49_000478"
     CDS             16086..16211
                     /gene="ykgO"
                     /locus_tag="WGO49_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ0305631.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
     gene            complement(16317..16517)
                     /locus_tag="WGO49_000479"
     CDS             complement(16317..16517)
                     /locus_tag="WGO49_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305632.1"
                     /translation="MIGRLFRIFFFFALAALIMNRLFSRRQKRALREVAEISARVLLG
                     AAAATLFWYLFMLYFKHIPDSY"
     gene            16660..17799
                     /locus_tag="WGO49_000480"
     CDS             16660..17799
                     /locus_tag="WGO49_000480"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ0305633.1"
                     /translation="MSQNASVGIVTPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPVVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSHNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            17796..18377
                     /gene="metW"
                     /locus_tag="WGO49_000481"
     CDS             17796..18377
                     /gene="metW"
                     /locus_tag="WGO49_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ0305634.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            18494..19339
                     /locus_tag="WGO49_000482"
     CDS             18494..19339
                     /locus_tag="WGO49_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ0305635.1"
                     /translation="MKNKTSSLLLWLAAMMLTACSPSKEDKTKENGASAASSTASAAS
                     SSAPQTDLQPAASAPDNVKQAESVPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(19389..19949)
                     /gene="efp"
                     /locus_tag="WGO49_000483"
     CDS             complement(19389..19949)
                     /gene="efp"
                     /locus_tag="WGO49_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ0305636.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKIEIDTRTGEFRKRA"
     gene            complement(19992..21140)
                     /gene="earP"
                     /locus_tag="WGO49_000484"
     CDS             complement(19992..21140)
                     /gene="earP"
                     /locus_tag="WGO49_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ0305637.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWTD
                     DVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTALVPDIVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSLQESVQKYFWFMGFSEKSGGLIRERDYRNA
                     VRFDTEALRERLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(21159..21437)
                     /locus_tag="WGO49_000485"
     CDS             complement(21159..21437)
                     /locus_tag="WGO49_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305638.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKLQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMLLFIPIILLIVYLFHYFGAF"
BASE COUNT         5355 a         5493 c         5476 g         5345 t
ORIGIN      
        1 tcagacggcc ttttcattaa caaataaaag gccgtctgaa acgattacag atccaacaac
       61 aggcgggctg ggtcttccaa cgcgtcttta atggctacca aggtcaatac agcttcgcgg
      121 ccgtcaatga tacggtggtc gtaagacaga gccagataca tcattggacg gacaacaact
      181 tggccgtttt caaccacagc gcgctcttta gtggcgtgca tacccaaaat cgcagattga
      241 ggcggattga tgatcggggt ggacatcata gaaccgaaag taccgccgtt ggtaatactg
      301 aatgtaccgc cggtcagatc ttcgatagcg attttgccgt ctttggcttt tttcgcgtaa
      361 tcaacaattg cttgttcgat gtcggcaatg ctcatttggt cggcatcgcg caggattggc
      421 acaaccaaac cgcgtgggct gccaatcgcg ataccgatgt cgaagtagcc gtggtacaca
      481 atgtctttgc cgtcaacaga agcattcaca accgggtatt ttttcagggc tgcaacagcg
      541 gctttaacga agaaggacat aaagcccagt ttcacgccgt gttctttctc gaatttttct
      601 ttgtacttcg cacgcaagtc catgattggt ttcatgttga cttcgttgaa tgtagtcaga
      661 atggcgtttt cttgttgaga agccaagagg cgttctgcaa cacgggcacg caggcggctc
      721 atcggtacgc gttcttcagg acgtgcgccg gcaggaagtg caacggcagg agcagcggct
      781 gcggcaggtt tggctgcggc attttgtacg tcttctttca atacgcgacc gtcacggccg
      841 gaaccttgca atgcgttcac gtcaacaccg ctctctgcag ccagtttggc ggcggcaggc
      901 atagcggcgt tgttttgtgc agcagcagga gcggcggccg gggcagcttc ggcaggagcg
      961 gcggcaggtg cttcagcagc ggcagtagca gcggcaggtg cttcagcagc ggcagtagca
     1021 gcggcaggtg cttcagcagc ggcagtagca gcggtatcga tacgtgccaa aacttggtcg
     1081 gcaacaacgg tttcaccgtc ttgcgctacg atttcaacca atacgccggc ttgtggagaa
     1141 ggtacttcca aaaccacttt gtccgtttcg atatcgatca ggatttcgtc acgggcaact
     1201 gcttcgccaa cttttttctt ccattccaag agcgtgcctt cagatacgct ttcagacagc
     1261 ataggtactt ttacatcaat aatcattttg tgtctccaat ggccctttca gacggcctgt
     1321 tgtgtgtttt tcattctgca tatgggtgtt ccgcctgagc agcctcagac ggaatgctac
     1381 ttggcttaca atgccaaagc gtcttcaacc aattgtttca attgagcaat gtgtttgctt
     1441 gagtagccca ctgcaggcga tgcgctgctt ggacgaccgg cataagacag tttttgctct
     1501 tcgctgataa cgtcttcgat gcggtggcgg atttggtaga acgcgccttg gtttttcggc
     1561 tcttcttgtg cccaaaccac agattttgcg ttcggatatt tcgccagttc ggctttaacc
     1621 tcgtcgtatg ggaacggata cagctgctca acgcggacaa tagcgacatc atcttccagt
     1681 ttacgttcgg ctcgaccggc ttccaagtca tagtaaacct gaccggcaca caatgccacg
     1741 cgtttcacgc tgtcgttgct tgcgcgttcg gcggtatcgc cgataaccgg acggaaagtc
     1801 gaaccttcgg tgaagttttc cagcgggctc attgcccctt tgaatcgcaa caggcgtttg
     1861 gacatgaaaa tcactagcgg tttgcggtat gaacctaaga cttgacgttg caagaggtgg
     1921 aacatttgcg acgcttcaga cggcatgatg acttgcatat tgttctcaga acacagttgc
     1981 aaccaacgtt ctacgcgtgc agaagagtgc tcgggacctt gaccgtcgta gccgtgcgga
     2041 aggatggtgg tcagaccgca caaacgaccc catttggttt cgcctgaaga caggaattgg
     2101 tcaatagtca cttgcgcgcc gttggcgaag tcaccaaatt gagcttccca aatggtcagc
     2161 ttgtcaggcg cggagcaggc aaagccgtac tcgaacgcca tcacggcttc ttcgttcaaa
     2221 atggagtcga taaccaagaa ctcgcccata ccttcgccca tatggcgcag aggaacataa
     2281 gtaccgtcat cccatttttc acgtttttga tcgtgcaata ctgcatgacg gtgtgagaat
     2341 gtaccgcgcc cggagtcctc accggagata cgcacgccat gacctttggt tacgaggctg
     2401 gcgtatgcca aggtttcggc cataccccaa tctatggcct gtttgccgga tgccatggct
     2461 ttacgcgctt caatcacacg ttttgcagtc ggatgcaggg caaagccttc cggtacggcg
     2521 gtaaacttct cagtgagacg ctcaatatct gcggcaggca aaccggtttc gatgtgttcg
     2581 cgccaatctt tgccttggta tttgctccag tcgatttgcg tgcgttgaaa gttgctcaac
     2641 gttgtttgct cgacgtgttc gcctttgtcc aaagcatcac ggtaggcttg gatgtaaccg
     2701 tctgcctcgg cttgggttac cacgccttcg gcaatcagtt gctcggtgta caaagcacgc
     2761 gcaccggggt gttgcgatac ttttttgtac atcatcggtt gggtcaaggt cggatcatcg
     2821 ccctcgttgt gaccccattt acggtagcag acaacgtcaa tcacgatgtc tttatggaat
     2881 tttttgcggt aatccaaagc ggcttggatg gcaaagcaaa cacgctcagg atcatcgccg
     2941 ttcacatgga taaccggagc ggaaaccatt tttgcgatat cggtacagtg tacggttgaa
     3001 cgggtatcgc ggatatcgga agtggtaaag ccgatttgat tgttgatgac gatatgaacc
     3061 gtaccgccgg tggtataacc gcgcgtttta gacaggttga atgtcgcttg gttgacaccc
     3121 aaaccgataa atgcggagtc gccgtgaatc aatacgggca agactttatc gcggccgttt
     3181 tcgcccaaac gtttttgctt ggcgcgtgca gaaccttcca ctaccggatt gacgatttcc
     3241 aagtgtgacg ggttgaacgc caaagaaacg tgcatcggac catgtggagt ggcgatgtcg
     3301 gagctgaagc ccatgtggta tttcacgtcg ccgctaggca atttgatttc ggcacggcct
     3361 tcaaactcag cgaacaaatc tccgggtttt ttacccaaaa tgttcaccaa aacattcaga
     3421 cggccacggt gcgccatacc gatgatgact tcttccacgc cgtctttacc ggcgttttga
     3481 atcaggtagt tcaaaccggc aatcgcgctt tcgccgcctt cgacaccgaa acgtttctga
     3541 ccgacatatt tggtatgcag ataacgttcc aaagtctcgg cagcagtcat ctctttcaag
     3601 atacggcgtt tttgatcggc attgtaatgc ggtgtggaca atacgctttc aaaataattg
     3661 cgtacccagc ggcgctcttc ggtattggga atatagatat attccaatgc gatgtggccg
     3721 cagtaggttt gtttgaggtt gctgatgatt tgggacaaag gcagtttgcc gcgattggca
     3781 aaatcgccct cgcccatatt gaattgaagc gccatatcgg catcagacag accgtggaat
     3841 ttcggatcga gggcttcaat atcgcgcggg gggatacgtt tgagcggatc aagttgggct
     3901 gcacccacgc cttggatacg ataggcagaa atcagccgta aaacgctgac ttgcttcttc
     3961 agcattgcct catccgcacc gcccgcaacg gcagatgcaa ttttcttttt cgccaaagta
     4021 acaaatgatt cgcgaatcgg tgtgtgtgcg acatcgacag caaccgtccc cggctgtttg
     4081 ctcaaatcgg tgaaatactg cttccatttt tcatcaaccg catcggggtt ttccaaaaaa
     4141 gcctcgtaca attcctcaat gtagggtgcg ttcgaaccga acaggtaaga gaaattgagt
     4201 ttttcgtcca tcatggcgtg cgctcttttc tttaaaatat aaacaaaggg tagaagtccg
     4261 cgtcctgacg gctgaactga tgccgtctga aaagacattt tattctaccc tattttaaac
     4321 cggattgcct gtattgattt catccgacta caatcaaatg tatacagaaa gcaatccgat
     4381 tctctaatat tgcctgtttg acaatatatt aatgtatgtt acctctctcc tgccggcaca
     4441 taatcgcggc gttccgaacc ggtataaagc tggcgcgggc ggccgatttt cagcgaagga
     4501 tcgctgatca tctcgtgcca gtgcgaaatc cagcccacgc tgcgcgacag ggcgaagatg
     4561 acggtaaaca tttcggtcgg gatgcccagc gcggacagga cgatgccgga atagaaatcg
     4621 acgtttggat acagcttgcg ttcgataaag aacgggtctt tcagcgcaat ctgttccaat
     4681 tccatcgcca gtttgaattt cggactgtct tccaagccca attccttcaa aacttcatag
     4741 caggtttcgc gcataatgct ggcacgcgga tccatattgc ggtacacgcg gtgaccgaag
     4801 cccatcagac ggtatttgcg ttgtttcaca ccttccatgt atgcggcaac attagacaca
     4861 tcgccgattt cgtccaacat tttcaacacg gcttcgttcg caccgccgtg cgaagcaccc
     4921 cacaggcagg cgataccggc agcaatacag gcaaacgggt tcgcacccga agaccctgcc
     4981 agacggacgg ttgaagttga ggcgttttgc tcgtggtcgg catgcaaaat aaagatgcgg
     5041 tcgagcgcgc gtgccaaaac gggattgggt ttgtagtctt cacacggcgt ggcgaacatc
     5101 atatgaagga agttttcgga ataagaaaga ttattcttcg gataattgaa cggcagaccg
     5161 tttgaatagc ggtagcacat tgccgcaatg gtcgggattt tggaaatcag gcggtaaatc
     5221 gcgattttgc ggtgttcggg attgctaatg tccaagctgt cttggtagaa cgcagacagc
     5281 gcaccgacca cgccgaccat catcgccatc ggatgcgcgt cgcggcggaa cccccggaag
     5341 aaccaagtca gctgttcatg caccatcgtg tggcggcgga cggtattgtc aaattctgcc
     5401 ttttgctcgg gagtcggcag ttcgccgtaa atcaacaggt agcagacttc caaataatcg
     5461 gacttttcgg ccagctgctc gatggggtat ccgcgataat aaagcaagcc ttgatcgccg
     5521 tcgatgtaag taattttaga ctcacagctt gcggttgaaa caaatccggg gtcgaaagaa
     5581 aacaaacctg tattttttgt cagcccccga atgtcaacca catcgtgccc gatgctggct
     5641 tccaataccg gcagctccaa acctgcctga cccggtacgt tgagtttgat tgatttggac
     5701 atattattgc tcctttgcaa gttataaccc acgttactgc atccgcagca cgttctttgc
     5761 cttcagacgg cttttgcatt ttgaaatctg cctgcaggca gatttcagat gcctcatgcc
     5821 cgtctgattt tttcaagcat cgggataagg tgccctttgt ccgtttccga atgcccgtta
     5881 atcaaggcaa gcaattcttg atcttgaaat tcaaggattt cggaaaactc ggacagctct
     5941 ttatcgctca aatgctcgaa ttctttttcc ataaacctgc cgaagattaa atctaattcc
     6001 aacaatcccc ggcgggtttg aaaacggatt ttccgtttgg caatatcgtc aaaaaccatc
     6061 atttcttaaa cgacccgttt caacataatc tctttaatct taccgatggc tcgggtcgga
     6121 ttcaagtgtt taggacatac gtctacgcag ttcataatgg tgtggcaacg gaacaaacgg
     6181 tatgggtcgt tcagattatc caaacgctca ttagtgatgg tatcacggct gtccgcaatg
     6241 aaacggtaag cattcagcaa accggacgga ccaacgaatt tatcagggtt ccaccaaaat
     6301 gacgggcagg cagtcgaaca gcaggcgcac aaaatacact cgtacaaacc gtccaactct
     6361 ttacgctctt cctgagtttg cagacgctct ttgtccgcat caatcggatt atcgttgaca
     6421 acataaggtt tgacggaatg gtattgtttg aagaactggg tcatatccac aatcaggtcg
     6481 cggataacag gcagacctgg cagaggacgg attttaaccg gctgtttcaa gccacgcaga
     6541 tcggtcaaac acgccaagcc gtttttgccg ttgatgttca taccgtccga tccgcaaatg
     6601 ccttcgcggc aggagcggcg gaaagacaag gtatcgtctt gtgctttcag gcgtaccaaa
     6661 gcatccaaaa gtttcacgtc ggtcggttcc aattccaact cgtaacgctg catataaggc
     6721 ttggcatcaa catccgggtt gtaacggtaa atttcaaaac tcattttttc catgagaagt
     6781 gttccttttc tcatcaactg cccaatgatt ttgtattata aattcaagca gttcaatgtg
     6841 ggtatggtta gatttcagac gagcttatga ggcgaagacc gtctgaaaac gcatcaataa
     6901 acgcgcttgg ccggtttgat gtattccacg ctcaaaggct tggtatgtac cggtttgtaa
     6961 gacaaagtat tggtatctga atggtacagc gtatgtttca tccagttttc atcatcgcgc
     7021 tcaggatggt cgtctgaagc gtgcgcaccg cgtgattctt tacgtgcttc ggcagacacc
     7081 aaagtcgctt tcgccacttc aattaggtta tccaattcca aagcctcgat acgcgcggta
     7141 ttccacactt tgctcttgtc tttgatttcg gtacgtttca cacgctcggc aatcgccatg
     7201 acttctcgaa cgcctttgct cagaatctca tcagtacgga acacgccggc gtgcaattgt
     7261 acggagcgtt gcagttcgcg gcgcaatgca tcaacgtttt caccatcggt ttggctgtcc
     7321 aaacgctcga tacgttggcg ggtcaactca ccagcattag caggcaaagg tttccagtcg
     7381 ctttgctctt tgatgaattt aatcatgctg tcgccggcag ctttaccgaa taccaccaag
     7441 tccaacaggg agttggtacc caagcggttc gcaccgtgta cggaagcaca agcgcactca
     7501 cctgccgcat acagaccttt tacaggcact tcgtattcgt cgccttgagg aacgacaact
     7561 tcgccatggt agttggtcgg aataccgccc atcatatagt gggtagtcgg cacaacggga
     7621 atcgggtctt taatcggatc gataccggcg aactgaatgg aaatctcgcg gatgcccggc
     7681 agtttttcca taattttttc tgcgccgata tggtcgattt tcagtaagac atggtctttg
     7741 tttttaccgc agccgcgacc ttcgtagatt tccatcgcca tcgcgcggga aacaacgtcg
     7801 cgagaagcca agtcttttac ggtcggcgca tagcgttcca taaagcgttc gccgtcggca
     7861 ttcaacagaa taccgccctc gccgcgtacg ccttcggtaa tcaacacgcc cgcacctgcc
     7921 acgccggtcg ggtggaattg ccagaattcc atatcttcca acggaatacc cgcacgcgcg
     7981 caaatgccca aaccatcgcc ggtattcata taggcattgg tagaagaagc ataaatacga
     8041 ccgccaccgc cggtagcaaa catcacagct ttagcgtgga aaatataaac ttcgccggtt
     8101 tccatttcca tggcggttac gccgacgaca tcgccgtttt catcacgaat caaatcttgt
     8161 gccgtccatt ccacaaagaa ttgcgtattg gcacggacgt tttgttggta caaagtatgc
     8221 agcatcgcat gacctgtacg gtcggcaacc gcacaggcgc gttctaccgc gcgtttaccg
     8281 tgttcggcag tatggccgcc gaaaggacgc tgataaattt taccgctttc cacacggtca
     8341 aaaggcatac ccatgtgttc caactcaatt acggcttcag gcgcggcgcg gcacataaac
     8401 tcaatcgcat cttggtcgcc caaccagtcg gaacctttca cggtatcgta catgtgccag
     8461 tcccaacggt cttcctgcac attacccaga gaggcggaaa taccgccctg cgccgctacg
     8521 gtatgcgaac gggtcgggaa cactttagac aaaacggcac aattcagacc ggatttggat
     8581 aattggaggg ctgcgcgtaa acctgcacca ccaccgccga caatcacggc atcaaacttg
     8641 cgaacaggaa aacccatact tacccccaaa ttactttaat tgaatacacc aagcagccga
     8701 ccagccagac gatggtggca acctgcaaaa acaaacgcac gccgaagggt ttgatatagt
     8761 ccatccacaa atcgcggata cccacccaag cgtgcaagaa tacggcgatg aagctcactt
     8821 gggtaaatac ttttacccaa gtttgactaa aaaatgcctg ccatgccgaa tattctttag
     8881 gcagggaaaa tagaaccact aaaagtgcaa cggtataaat caacataata accgcagtcg
     8941 cacgttgcat cgcccaatcg cgcaaaccgt aatgggcacc ggtcaattta cgttctacca
     9001 taacaacgct cccaaaacga cagtcaaaac caatgcagaa gcaaatacgg ctttagcggt
     9061 attgcgcgca gtattcagct caaggccttt gtgcgcatcc aaaaataaaa agcggatacc
     9121 ggcgagagaa tggtgcagat aagcccacaa tacaccgatt aaaaccagct tgaccaaagg
     9181 atgggaaaca atggcacggt aagtttcaaa tgcagactct tgactcaggg taccggacag
     9241 gaaatacagc aggaaaggca gcataataaa cagcccgacc ccgctgatgc ggtgaaggat
     9301 ggaaactatc ccgggtatcg gcagacggat gttcggcaaa tccagataaa caggacgcgg
     9361 tttggcagac atagtcaatt cctctgtagt tatatatcga ctcaaaaacc gtaaacccat
     9421 tgctacactg ggttacataa acgttaattt acctgtttta tcccattttg aacagtgtat
     9481 tttaaaaaca aactgataga ttttgacaaa cacgcgtgga aacagcagat taaaagaaag
     9541 atttttagtt ttgccgcccc cttcccgaca cggtatcagc atccgacccc atccccaaaa
     9601 acaaaatgcc gtctgaaaaa atttcagacg gcattgtttt gccatatcgg tcaggctttc
     9661 aatttcgcat cggcatcgcg caacgcggta cgcagacctt cctcgataac gggatggtag
     9721 aacggcatat ccagcatttg cggaacggtc atcttcattt gatgcgccca tgccagcaag
     9781 tgcgccaaat gttcggcggc agggcctacg atttccgcgc cgataaagcg gccggtggct
     9841 ttttcggcat acaggcgcat atggcctttg ttcaccagca tcacgcggct gcggccttgg
     9901 tttttgaacg atacttcgcc gatgacaaat tcgtcggctt ggtattgcgc ggcaacctgc
     9961 gcgtatttca gaccgacaaa gccgatttgc ggactggtaa acaccacgcc gatggtgctg
    10021 cgccgcaaac cgccgccgat attcgggtag cggcccgcgt tatcgccggc aatcttgcct
    10081 tggtcggcag cttcatgcag cagaggcagt tggttggacg cgtcgcccgc aataaagata
    10141 tgcggaatgc tggtctgcat ggtcagtggg tcggcaacgg gtacgccgcg cgcgtctttt
    10201 tcgatattga tgttttccaa accgatattg tcaacgttcg gacggcggcc cacggctgcc
    10261 agcatatatt cggcaacaaa tacgcctttt tcgccgtcct gctcccaatg gacttctaca
    10321 ttgccgtctg catctagttt gacctcggtt ttagcatcca gatgcagttt caattcttcg
    10381 ccgaacacgg ctttcgcctc gtctgaaacg acggggtcgg aaatgccgcc gatgattccg
    10441 cccaaaccga aaatttcaac tttcacgccc aaacggtgca atgcctgacc cagttccaaa
    10501 ccgataacac ccggcccgaa cacggcaacg cgcttaggca gcgtatccca tgagaaaacg
    10561 tcatcgttga taatcaaacg attgcccaaa gactgccatt gcggcaggat gacgggacgc
    10621 gaaccggtag caatcacgaa actttttgcc gtaatttgag tatgctcgtc gatttggacg
    10681 gtatgctcgt cgataaattt agccgaaccc atgatgcgct tgtcggcagg ccactcttcc
    10741 acatcggcaa cgacaaagcc gacaaaacgg tcacgctcgg atttaacgcg ctgcatgacc
    10801 tcttcaccgt tgacgacgat gctgtctttg tccaaatgca cgccgaacgg gtcggtatgc
    10861 aatgcgtggt ggcgcgcttc tgcggcggca atcaagagtt tggaaggcat acaacccacg
    10921 cgcgcgcagg ttgtgccgaa cacattgttt tcaatcaggt aaacattatc cgaatgtaaa
    10981 cgggcattgc gaaacgcacc cataccggca gtaccgccgc cgattacgac gacatccgct
    11041 tgaatttttt tcataatctt tgtccttccg ttttcgtctg aaacattgcc gacacagaac
    11101 atatcggttt cagacggcat ttaaatattt tcccaatcaa aaacatcatt cagataaata
    11161 aaaacaaaat ttcgattgag tgcaacaata tccaaattct cttataaata tagagttatt
    11221 cggatcggca gtgtagaacc gcccgaataa ctctatccgg tatatcagac agtttaatca
    11281 aaccgcctta agcaaacagc tatcagtttt tagccaagta agcttccaaa tcttcgctgc
    11341 cgccgatgta tttgccgccg atgaagactt gaggggcagt catcttgccg gtaatggcgc
    11401 gaacggaagt aacggttgca tctttgccca atacgatttc ttcgtaagac aaacctttgt
    11461 cttgcaaagc ttgtttggct ttggcgcaga attggcaacc tggtttagtg aaaattgcca
    11521 cagactcttg agctttccaa tcaggagcaa cgaatttcag catagtatct gcatcggata
    11581 ctttgaacgg atcgcctggt tcttcaggct cgatgaacat tttttcaacc acgccgtcgt
    11641 taaccagcat ggagtaacgc caagagcgtt taccgaagcc caagtcttct ttaccgacca
    11701 gcatacccat accttcggta aattcgccgt tgccatcagg aatcatgtag atgttgtcgg
    11761 attcttcttc ggcagcccaa gcgttcatta cgaacgtatc gtttacagat acgcagtaga
    11821 ttgcgtcaac gccgttttct ttgaacgcgc cgaacaattc gttgtaacgc ggcaggtgtg
    11881 aagaagaaca agtcggggta aatgcaccgg gcagggagaa tacgactact tttttgcctt
    11941 tgaacaaatc atcggtagac acatctttcc aagtgtcgcc gacgcgggtg cggaatacta
    12001 cggaaggtac tttttgaccg gtacgatctt gcaaagccat tttaagctcc ttaatagatt
    12061 ggtttgagaa ataattgtga atcgctgggc attctacccc atgcaaaatg atttgtataa
    12121 tttatagttc aaataatata aattatttaa aactatcaaa cacaactccg atttgcctgt
    12181 ttcccacata taaagccgaa ttagaaaaaa acaagctgca atgaaaagat tttccggcaa
    12241 ctgcgccctt gcgccgcaaa tcaggacaaa ataccgtctg aaaggctttc agacggtatt
    12301 tattcatacg gtctcggctt atttcgccaa ttcggcacgc aatttgtggg ttacgttcat
    12361 catcacttgg agttgttcca gagtttcttt ccagccgcgt gttttcaggc cgcagtccgg
    12421 gttaacccac agacgttcaa ccggtacaac ctcgatggct ttgcgcaaca ggtgctccac
    12481 ttcggcttct gtcggtacgc gcgggctgtg gatgtcgtaa acccccgggc cgatgtcgtt
    12541 cgggtatttg aattcgccga acgcggtcaa gagttccatg tcggaacgtg aagtctcgat
    12601 ggtgatcacg tccgcatcca ttgcagcaat cgcaggcagg atatcgttga actcggagta
    12661 gcacatatga gtgtggattt gtgtgctgtc ttcgcaaccg gcagaggaca ggcggaagga
    12721 ttcgcccgcc cagttcaggt aggcatccca atcggcgcgt ttcagcggca agccttcgcg
    12781 gatggcaggt tcgtcaattt ggatgacttt gatgcctgct ttttccagat ccaatacttc
    12841 gtcgttcaga gccagtgcga tttgtttgca cacggtagag cgaggaatat cgttgcggac
    12901 gaaagaccat tgcagaatgg ttacagggcc ggtcaacata cctttcatcg gacgtttggt
    12961 caggctttga gcgtaagtag accaagcgac agtcatggct tcaggacggc ttacgtcacc
    13021 gaagataatc ggtggtttaa cacaacgtga gccgtagctt tgtacccagc cgtattgggt
    13081 gaatgcaaaa ccgctcaaca attcgccgaa gtattcgacc atgtcgttac gctcggcttc
    13141 gccgtgtacc agtacgtcca aatccagttt ttcttgttct tcaaccacca aggcgatttc
    13201 ttttttcatc gcggcttcgt aatcggcggc agacagttcg ccttttttga aggctgcgcg
    13261 tgcttggcgg atttcggtag tttgcgggaa agaaccgatg ttggtcgtcg gcagcagagg
    13321 caggttcaac catgcttgtt gcgctttgat acggtcggca aatggagatt tgcgttggtc
    13381 tgcgttggca ggcaaatcgg ccaggcgttt ggcaacgtct gcacggtgga tttcgctgct
    13441 gttggcgcgt gaatcggcag cagcttggct ggcggccagt tcttcggcaa cggaatcgcg
    13501 accttcgttc aatgcggctt tcagaacgcg caattcttgg gttttttgca gggtaaatgc
    13561 caaccaagag tacaggtccg gtttgttggc tttcagtttt tcttcaactg acaagtcaaa
    13621 tggagtgtgc agcagagagc aagagctgga aatccacaaa cggtcgccca gtttggcttg
    13681 cagaggctca acagtttcca aaactttgtt caggttggcg cgccagatgt tgcggccgtc
    13741 gatgatgcct gcagacagaa ctttgtcgta gtcggcaaat gcgtccagtt gctcgggagc
    13801 gcgtaccaag tcgatgtgca ggccgtcaac aggcagggat ttcaacaatg cggcgtgttc
    13861 ggcaacagaa ccgaagtaag tgctcagcag gattttggca cttactttgc tcaaagtagc
    13921 gtaaacgtct ttgtaggctt ctacccattc tttaggcaga tcgacagtca aagcaggctc
    13981 gtcgatttga atccattctg caccggcttc aaccaaagcg tttaggattt caacgtaaac
    14041 aggcagcagt ttttgcaaca ggctcaggcg gttgaattcg actgtgcctt tttctttacc
    14101 cacccacagg aaagtcaacg gacctacaac ggtcggtttg gctttcagac cgagggcttg
    14161 ggcttcttgc agttgttgaa catagtgttt ggcattggct ttgaattcgg tatcggcgtg
    14221 gaattcaggc accaagtagt ggtagttggt gtcgaaccat ttggtcattt cgatagcgaa
    14281 ttggtctttg ttgccgcgcg ccagttggaa gaattgttcc aaagacaggt tttggctgtc
    14341 gaaaccgaag cgggcgggaa tcgcgccggt ggcgacttgc aggtcgagga tgtggtcgta
    14401 gaaagtgaaa tcgcctacgg caacgaaatc ggcgttggca gcggcctggt gtttccagtt
    14461 tttctcgcgc aagtctttag caacagccag caattcttgc tcgctgattt ctttgcgcca
    14521 gtatttttct tgtgcgaatt tcaattcgcg gaaggcgccg acacgcggga agcctgagaa
    14581 atgtaatgtt gtcatgttga actctccagt tggattttgt aaggaccggt tcgatccaaa
    14641 tcggccggta ttgtaaatca gaatggctcg gatggggcgg tgttttgctt tttagacacc
    14701 ttagatgccg tctgaaactg tttgatacgg ctttaagggc gcacgcctaa aatatggcag
    14761 atggcgtaag tcagctcgct gcggttaagc gtatagaagt ggaaatcttt cacgccttcg
    14821 cgggacagga ctttgaccat atcgatggcg atacttgccg ccaccagatt gcgcgtacct
    14881 tggtcgtcgt ccaaaccttc atacatttgc gacagccagc ttgggatttt gacgttggtt
    14941 acttgcgcca ttttgccgag ctgcttgaag ttggtaacag gcaaaatacc ggggacgatt
    15001 tccacatcga tacccaacat cacgcagcgg tcgcggaagc gcaggtagcg ttccacatcg
    15061 aagaagaatt gggtgatgac gtggttcgcg cccgcatcga ttttgcgttt cagattgatc
    15121 aagtcggctt gagcggattt ggcttccggg tgtacctcag gataggccgc tacggaaatg
    15181 tcgaagtcgg caacggagcg caacagtttg accaaatctt cggcatagaa aggttttttc
    15241 tcgtagccgg gcagctcgtc gccgcgcagg gcgacaatgc ggcggatgcc gctgtcccaa
    15301 tagtctttgg cgatttggcg caattcgtcg ggagaagcgt caataccggt caggtgcggg
    15361 gcggcttcca agccggtttc ctgtttgatg cgtttgacga tgccgtgtgt gcggtcgcgc
    15421 tcgcctgagt ttgcaccgta agttacggaa acaaatttcg ggtgcaaggt ttgcaggcga
    15481 tggatggaat cccacagcat ggtttccatt tgttcgtttt tcggcgggaa aaattcgaat
    15541 gaaacgttga tgtcgccttt caaatcggaa aggctgttat ttaacgcatt gatttctttt
    15601 gcgtaattca tggctgcgta ccttttatta tcttttatat gatgtttcag gcaacataat
    15661 aaagttgcgc caaacatgag tcaatttcaa aattttcaga tttatcatga aaatttctaa
    15721 tatattacaa aaatagttaa atttccttgt acagcccttc taaactatgc tagagtttgt
    15781 tatgttataa cactgaaagc aaggcaatat atgaaaccga atattcatcc cgacaattac
    15841 cgaaccgtcc tttttttcga tagcagcgcc aatgaaggct ggttaatccg ctcctgtgcc
    15901 gaaacacacg gaaaaacgat ggtttggaca gacggcaaag aatatccgct gttttcgctg
    15961 gatacttctt ccgcatccca tcccgtctat actggcaaac agcgcaacgt caacaccgaa
    16021 ggccgtgcca gcaaattcaa ccaacgtttc caatccgtaa tgtcatcgtt tagaaaggac
    16081 aaataatgca ggttctgtct tcactcaaaa ccgccaaaca acgccaccgc gactgccaaa
    16141 tcgtcaggcg caggggcaaa gtttacgtca tctgcaaaag caatccgcgt tttaaagcgc
    16201 gccaacgctg accccctgcc cttcctgctt cggttgtaaa tcccctccta ccgcaagccc
    16261 ttttcaaaaa atgccgtctg aaacagaaaa atgcgtttca gacggcattt tttccgtcaa
    16321 tacgaatccg gaatgtgttt gaaatacagc ataaacagat accaaaacag cgtcgcggcg
    16381 gccgcaccga gcagcacccg ggcactgatt tcggcaactt cgcgcagggc gcgtttctgc
    16441 ctgcggctga agaggcggtt cataatcagc gcggcaagtg cgaaaaagaa aaaaatacgg
    16501 aaaagtctgc ctatcatgtc gggaagcgaa aagtatggtt cgggcgcggc ggcatgggga
    16561 aggatgtttc agcgcgtcag gctgcttgtc cgaccgtgtc aaaaagtcat gccgtttgcg
    16621 atataataac gctttttgcc gtatttgttg gaacaggata tgagtcaaaa tgcctcggtg
    16681 ggcattgtaa cgccccaaaa aattccgttt gaaatgccgc tggttttgga aaacggtaaa
    16741 actttgccgc gtttcgatct gatgattgaa acctacggcg agctgaatgc cgaaaaaaat
    16801 aatgcggttt taatctgtca tgcgctgtca ggcaaccatc atgttgcggg caggcattcg
    16861 gcggaggata aatatacggg ctggtgggac aatatggtag gacccggcaa accgattgat
    16921 acggaacgtt ttttcgtggt cggtttgaac aatctgggcg gctgtgacgg cagcagcgga
    16981 cctttgtcga tcaatcctga aacgggtagg gaatacggcg cggattttcc ggtggttacg
    17041 gtgaaggact gggtaaaatc acaagccgcg cttgccgact atctcggcat cgggcaatgg
    17101 gcggcggttg tcggcggcag tttgggcggt atgcaggctt tgcagtgggc gatttcctat
    17161 cccgaacgcg tgcgccatgc cttagtgatt gcgtctgcgc cgaaactgtc cacgcaaaat
    17221 atcgcgttca acgatgtggc gcgtcaggcg attttgaccg accccgattt caacgaagga
    17281 cattaccgca gccataatac cgttcctgca cggggcttgc ggattgcccg catgatgggg
    17341 cacattacct atcttgccga agacggtttg ggcaaaaaat tcggacgcga tttgcgttcc
    17401 aacggctatc aatacggtta tggcgttgaa tttgaagtag aatcctatct gcgctatcag
    17461 ggcgacaaat tcgtcgggcg gtttgatgcc aacacctatt tgctgatgac caaagctttg
    17521 gactatttcg atccggcggc ggatttcggc aacagcctga cccgcgccgt gcaggatgtg
    17581 caggcaaaat tctttgtcgc cagcttcagc accgattggc gtttcgcgcc cgaacgttcg
    17641 cacgaactgg tcaaggccct gattgccgcc caaaaatccg tgcagtatat cgaagtcaaa
    17701 tccgcacacg ggcacgatgc ctttttaatg gaagacgaag cctatatgcg tgcggtcgcc
    17761 gcctatatga acaacgttta taaggaatgt cagcaatgaa cctgcgcgat gatttgcaac
    17821 tgatttacga ccggataccc gaaggcagcc gcgtcttgga cttgggctgc ggcgacggcg
    17881 aactgcttgc cgcattggtc gaacacaaaa aatgcagcgg ctacggcatc gaaatcgaca
    17941 ccgacagcgt gattgccgcg atgtcgcgcg gcgtgaatgt tatccaagcc gatttggaag
    18001 aaggtttaac cgcattcaac gatcaaagtt ttgatgtgat tgtgttgagc caaaccatcc
    18061 aagcgatgca gaataccgaa aaaatcctgc gctgcctgat gcgcgttgcc aaacaggcga
    18121 ttgtcagctt cccgaatttc ggctactggc gcaaccgcgt ccaaatcgca ctcggcggac
    18181 atatgccggt ttccgaacgt atgccctacc attggtacga cacgcccaat atccattggt
    18241 gcacgctcaa agactttgat ttgttgtgcg ccaaaaacaa catccgcgtc ctcgaacgcg
    18301 ccgtgatgac gggcaacaga caggtcaaac atttccccaa ccttttgggc agccttgcgt
    18361 tttaccgcgt cggctaagac aggttgagaa ccttgtcggc aataacaggt tcttcggttc
    18421 tattaacaac aggccgtctg aaaaatgaaa cggtaggttt tcagacggcc tctttaatac
    18481 tggaaacaat agaatgaaaa acaaaacctc atcacttctc ttatggcttg ccgcaatgat
    18541 gctgaccgcg tgttccccga gcaaagaaga taaaacgaaa gaaaacggcg catccgccgc
    18601 ctcgtccacg gcatccgccg cttcgtcttc cgcgccccaa accgatttgc aaccggccgc
    18661 atccgcccct gataacgtca agcaggcaga aagcgtgccg ccgtcaaatt gcaccgacct
    18721 gcaccccgcc accggcattg acgatctcat gcagcaaatc gccgaacaca ttgactcgga
    18781 ctgtctgttt gccctttccc atcacgaact ggaaacccgt ttcggcttac ccggcggcgg
    18841 ctatgacaac atacagcggc tgctgtttcc cgacatccgc cctgaagatc ccgactacca
    18901 tcagaaaatc atactggcaa ttgaagactt gcgttacgga aagcgcacga tcagccggca
    18961 ggcacaagat gccttgatgg aacaggaacg ccgcctccga gaagcgacgc tgttgctgat
    19021 acagggcagt caagaaaccc gcggacaagg cgaggagccg aaacgcacgc gttattttga
    19081 agtttcggca acccctgcct attcgagccg gcacaacaac ggacttggcg gcaatttcca
    19141 atacatcggc caattgcccg gctatctgaa aatacacgga gaaatgcttg aaaaccaatc
    19201 actcttccgg ctgtccaacc gtgaacgcaa tcccgacaaa ccgtttttag acatccattt
    19261 tgacgaaaat ggcaaaatca cgcgtattgt cgtttacgaa aaaaacatct acttcaatcc
    19321 aaacttgggg cgaagataaa aaaatgccgt ctgaaacgtt ttcagacggc atttttcaat
    19381 gcaacaaatc aagcgcgttt gcggaattcg ccggtgcggg tatcgatttc gattttgtcg
    19441 ccgttttcga tgtaagacat cacttggatt tcggtgccgc cgaccaggcg cgcggttttc
    19501 atcactttgc cggaagtatc gcctttgacg gcaggctcgg tatactcgac ttcgcgtacg
    19561 atgatggtgg gcagttctac ggagatgggg ttgccttcgt agaaggtaac ttcgcattgg
    19621 tcttccatgc cgtcaacgat gaatttcaac gcgtcgccga tgttgtcggc ttcgatttcg
    19681 tattggttga attcttcgtc cataaagacg tacatcggat cggcaaagta gctgtacgta
    19741 cagtttttgc gggacaggat gaccacgtcg aatttgtcgt cggctttgta aatggtttcg
    19801 gaagccgcgc cggtcagcag gtttttcagt ttcatgctga ctttggcgga agagcggccg
    19861 cctttgatgt attcggtttt ttgaacgacc ataggatcgt tgccgaccat aaatacattg
    19921 ccggcgcgca gttcttgtgc tgttttcatt gtgattttcc aatcaaatcg gttgtcgtaa
    19981 aacgcactat tctagcgtat tttttgatgc tttgaaacaa aggcggcgag tttttcggat
    20041 gcggaaggct gcccgaaaag ataacggctc caatcctccg cgccttgccg ccagccgttt
    20101 tgatgttgtt gcaggatttg ccaacattcg aggcgttgcg tggcggataa agcctctccg
    20161 ccgttgaggt cgtctgaaag gcggcggtgt accgatgcgg tttcgggcga gtaaaatcca
    20221 tgcgccttat cccaaaaggc gtggagttta tcgagatgga cattctcgtc ttgcgggtag
    20281 atgtgccaaa agaagggttt gccggcaagc tgggcgcgca cgaaactgtc ttcgccgcgt
    20341 atcacggcgc agtcggcgag gtgcagcagt ttgtcgaagt cctgttgcgg cacgaaaggg
    20401 attttgacga ggcggacgga tgccgtctga aaaacatcgc cgtcgttttg cagggcatct
    20461 tgcggaataa cgccgctttg tttgaggctg tcgatgattt gcgtccccgc cagcaacagt
    20521 gtcatcgggc tgcctgcctg tcgccacatt tccagccact ttgcccaaac atcgctccga
    20581 tagccgaaaa gcagccattc gggggcgttt ttttcgggaa gcatcagccg ctctcgcaag
    20641 gcttcggtat cgaaacggac ggcatttcgg taatcgcgtt cgcgtatcaa cccgccgctt
    20701 ttttcgctga aacccataaa ccaaaaatat ttttgaacac tctcctgcag cgaaggcatc
    20761 agatgcagcc tttcattgct ttcttccgcg ctcaaatatt cccaattcag ccaaagcggc
    20821 ttgtgtcggc ggatgatgtg cagcacattt tcgggcaggt cgcaggcaaa agtttcgata
    20881 acgatatcgg gaacaagggc agtatcgata tccgccgcat cggaatgcca agtgcggaca
    20941 tgaatatcct gatgaacgca gggaacatcg ggcaaatcag ggcaaagcgc acgcaaggcg
    21001 gacacatcgt ccgtccacaa atgcacctgc caaccgagtt cgcggtgcaa aacacgggcg
    21061 agccgccacg aaacgccgat gtcgccgaaa ttgtcgatga ccttgcaaaa aatccaacag
    21121 acaaaaggag gagtattcat agaaataacg gaaacaagct aaaacgcgcc gaaatagtgg
    21181 aacaaataca caatcagcaa aataatcgga ataaacagca acatagtttg acgtttcaaa
    21241 tcaaactctt gtttcaaatg atgcttgatg gtgtcgaaaa ggacgaattt aaacaaaaac
    21301 gtcgcttcgc aggcaaggat gatgccgttg atcagatgct ggagtttgtc ttcgggcgga
    21361 ttggcggcga aaacggcaag cgtcgccaac gggatgaggc agacggcaag ccaacggaca
    21421 agcaggtttc gagacattgc aaggctttca aaaaaagcaa aacgccatta tcgccccaag
    21481 cgcacgcctt gtctaacgga cgcaccggag atgccgtctg aagccttgcc gcaggaaaac
    21541 accgcaccgc cgaaccttta aatacgcccg agacgggcgt tccccgaaaa atcggacgct
    21601 gccgccgatg cctgaggaca aggcggcagc aggcacgggc atattgaaaa cccggcgcgt
    21661 cggtttgaa
//
LOCUS       DAWFAD010000017        21064 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWFAD010000017 DAWFAD010000000
VERSION     DAWFAD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21064
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.017"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..176
                     /locus_tag="WGO49_000486"
     CDS             <1..176
                     /locus_tag="WGO49_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305639.1"
                     /translation="LVLIFVNPLYNVGIGSGALLGHWVTQYSGISCIGIAGMLVSAAG
                     LWVCLNLNRHIRT"
     gene            305..1741
                     /locus_tag="WGO49_000487"
     CDS             305..1741
                     /locus_tag="WGO49_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ0305640.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSAQNLLLLAAGMGWLYHI
                     GPVFAAIIVLYSSCVYLLGELLRSDRENTRRFWLGCGIAASLTVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFNGWGVLAGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RAANIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLSFVVFNTASPD
                     DAGAVFNALFANANGWNAPQQANMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPISIILLLIIVLAPSGIPGFIYANF"
     gene            1752..2738
                     /locus_tag="WGO49_000488"
     CDS             1752..2738
                     /locus_tag="WGO49_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ0305641.1"
                     /translation="MKHFLSLFASILMSALIAAWFAQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYTLSDGIKAFLSGETPPTAQDGGSADMPPEAAASEAAPQTGEI
                     QEWKQDTDTAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYP
                     SFFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDR
                     ILEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLKGKIILIPTAHTLSGGKD
                     RYTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2735..3928
                     /locus_tag="WGO49_000489"
     CDS             2735..3928
                     /locus_tag="WGO49_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ0305642.1"
                     /translation="MNSKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDG
                     IASKQRVSLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTGAALPLTIHTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTQWSKWRADRMNDLAQTGADLVILAYGTNEAFGDNID
                     IADTEQKWLDTVRQIHDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRIA
                     RQGQTMFWSWQNAMGGVCSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
     gene            4177..5730
                     /locus_tag="WGO49_000490"
     CDS             4177..5730
                     /locus_tag="WGO49_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ0305643.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASAGLSKELAGLKAQTPVEKIIWTDKSRPAGEMAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(5820..7847)
                     /locus_tag="WGO49_000491"
     CDS             complement(5820..7847)
                     /locus_tag="WGO49_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ0305644.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQAQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLENLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGNLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            8136..10142
                     /locus_tag="WGO49_000492"
     CDS             8136..10142
                     /locus_tag="WGO49_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ0305645.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TAPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGVIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLVGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            complement(10265..11356)
                     /gene="mltB"
                     /locus_tag="WGO49_000493"
     CDS             complement(10265..11356)
                     /gene="mltB"
                     /locus_tag="WGO49_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ0305646.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRVNEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAVIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(11505..12932)
                     /locus_tag="WGO49_000494"
     CDS             complement(11505..12932)
                     /locus_tag="WGO49_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0305647.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKRHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            13117..17244
                     /gene="mfd"
                     /locus_tag="WGO49_000495"
     CDS             13117..17244
                     /gene="mfd"
                     /locus_tag="WGO49_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0305648.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHALPDLAVNRQSDEPLQALKDFQTAFEGRILLCAESLGRRETMLGFLQQNGL
                     KAKSVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDSLKAAAVSTESSLPLGTSNLHGQIRQQPAPSP
                     VGEGWGEGKAVAAQTEFPASATNPLPSPLPQEREQSAAAVSDDLKTKSSLHPVANNLH
                     GQIRQQPTPSPVGEGWGEGKAVAAQTEFSAAATKPLPSPLPQEREQSAAVVSDSLKAA
                     AVSTESSLPPGKSNLHGQIQQQPAPSPVGEGWGEGKAVAAQSAIAVITESDLYQYVAR
                     SRVHNRRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLITMDLGGETNEMMLL
                     EYAGEAQLYVPVSQLHLISRYSGQAHENVALHKLGSGAWNKAKRKAAEKARDTAAELL
                     NLYAQRAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVC
                     GDVGFGKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSR
                     FNNSKATKAALEGLADGTVDIVIGTHKLVQDNIRFKSLGLVIIDEEHRFGVRQKEQLK
                     RLRANVDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREA
                     VLRELKRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQ
                     QRFNVLLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTP
                     EYITKDAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTE
                     MLKQAVRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCET
                     VQQINAIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNN
                     NVDPTEIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            17393..17776
                     /locus_tag="WGO49_000496"
     CDS             17393..17776
                     /locus_tag="WGO49_000496"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ0305649.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVVCLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            17798..18640
                     /gene="kdsA"
                     /locus_tag="WGO49_000497"
     CDS             17798..18640
                     /gene="kdsA"
                     /locus_tag="WGO49_000497"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ0305650.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVAAMAETGNVINIKKPQFLSPSQMKNIVEKFREAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            18655..19095
                     /locus_tag="WGO49_000498"
     CDS             18655..19095
                     /locus_tag="WGO49_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ0305651.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     SVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKEAV"
     gene            19142..20428
                     /gene="eno"
                     /locus_tag="WGO49_000499"
     CDS             19142..20428
                     /gene="eno"
                     /locus_tag="WGO49_000499"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ0305652.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDASEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDAGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            20442..20720
                     /gene="ftsB"
                     /locus_tag="WGO49_000500"
     CDS             20442..20720
                     /gene="ftsB"
                     /locus_tag="WGO49_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ0305653.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(20761..21051)
                     /locus_tag="WGO49_000501"
     CDS             complement(20761..21051)
                     /locus_tag="WGO49_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ0305654.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYRKPPLAFLGRDEILPCCCYVEGDVRLDCGLSGEDEGLSDSLLK"
BASE COUNT         5022 a         6398 c         5252 g         4392 t
ORIGIN      
        1 gccttgtcct gatttttgtt aatccactat acaacgtcgg tatcggcagc ggcgcactgt
       61 tgggacattg ggtaacgcaa tactcgggca tttcctgcat cggcattgcc ggtatgctgg
      121 tatcggcggc aggtttgtgg gtctgcctga atttgaaccg ccatattcga acctgaccgc
      181 gcgatgccgt ctgaagcccc tcccgccctt cagacggcat tttgattgaa gaaatatccg
      241 cccctgctta aaataacagg ctttgccgtg ttttaacgcc tattttttgt tccaaggatg
      301 gtttatgccg ttgctgtctg tcgagttcgc actgttcttt ctcgctttcc tgccgattta
      361 ctggggcttg gcgaaatacc cgtccgccca aaacctgctg cttctggctg ccggtatggg
      421 ctggctctac catatcggcc ccgtatttgc ggcaatcatc gtcctttatt cctcctgcgt
      481 gtaccttttg ggcgaactgc tccgttccga tcgcgaaaat acgcgccgct tttggctggg
      541 gtgcggcatt gccgcctcgc tgaccgtctt gggctttttc aaatatttcg actttttccg
      601 gccgctgatt gcccaatatg ccggaaaagg cggcgcaatc gacatcctga tgccgctggg
      661 gctttcgtat tacaccttcc agtcgctcgc ctatcttgtt tactgcttcc gcgccccgca
      721 cgccgcgcgt ttcagctggc acgagctgct gctgcacctg agttttttcc ccaccgttac
      781 ctccggcccg attatccgcg ccgccgcatt caaaagcgca gacggcgagc aggcaggcgc
      841 attggcgcaa atccgtaccc gccaaccgcg ctcacccgtc cgccccgcac tcgccgtttc
      901 cctgattctg ctgggtattg ccaaaaaatg gtggctggcg gggatgctcg cagaaaactg
      961 ggtgtcgccc gtgtttgaaa accccgccca attcaacggc tggggcgtgt tggcgggcgt
     1021 gtacggctat accttccaac tcttcctgga cttttccgga tattccgatt tggttatcgg
     1081 catggcgatg ctgctgggct tccgactgcc caaaaacttc tccgcaccgc ttcgtgccgc
     1141 gaatatccgt gcattttggg acaaatggca catcagcctt tccacctgga tacgcgacta
     1201 catctacatc cccttgggcg gcagcaaaaa aggctttttg cggacacagc tcaacctgat
     1261 ggcggcaatg gtactctcag gcatctggca cggctacggc tggaacttcc tcatttgggg
     1321 cgcgctgcac ggcacggcgc tggtgctgct caacacgggc gatcgctatt tcggacgcaa
     1381 cgcgctatgc cgtctgaaat accttgcgcc gctctcgtgg tttgttacct tccatttcgt
     1441 ctgccttagc tttgttgtct tcaataccgc aagccccgac gatgcaggcg cagttttcaa
     1501 tgccctcttt gccaatgcca acggctggaa tgcgccgcaa caggcaaaca tgctgctgct
     1561 tgcctcgttt gcatccgtga tgctgctcta cccttacctg caacgcgctt tcgacggcgc
     1621 ggtcaaaggt ttggaaaaaa tcccgatgtg gctgtggttt atcccgattt ccatcatcct
     1681 gctgctgatt atcgtccttg ccccctcggg gatacccggt tttatttatg ccaattttta
     1741 agggtttgga catgaaacac tttctttccc ttttcgcttc catattgatg tctgccctga
     1801 ttgccgcgtg gtttgcccaa aacccgatca atgcctactg gcagcagacc taccaccgca
     1861 acagcccgct cgaaccgctt gccgcctacg gatggtggcg gagcggtgcg gcgttgcaag
     1921 aaaacgccta caccctttca gacggcatca aagccttcct gtccggcgaa acgccgccga
     1981 cggctcaaga cggcggttcg gcagatatgc cgcctgaagc cgccgcatcc gaagccgccc
     2041 ctcaaaccgg cgaaatccaa gaatggaaac aggacaccga caccgccgcc gtccgcagcg
     2101 gcgacaaagt ctttttcgcc ggcgactcgc tgatgcaggg cgttgcaccc ttcgtgcaaa
     2161 aaagcctgaa acagcaatac ggcatcgaat ccgtcaacct cagcaaacaa agcacggggc
     2221 tgtcctaccc ctcattcttc gactggccga aaacgattga agaaaccctg aaaaaacatc
     2281 ccgaaatcag cgtgctcgcc gtcttcctcg gcccgaacga cccgtgggat ttccccgtcg
     2341 gcaaacgcta cctcaaattc gcttccgacg aatgggcgca agaatacctg aaacgcgtcg
     2401 accgcatcct tgaagccgca cacacgcacc gcgtccaagt cgtctggctc ggcatcccct
     2461 acatgaaaaa agccaagctc gacgggcaga tgcgctacct cgacaaactg ctttcggaac
     2521 atttgaaagg caaaatcatc ctgattccca ccgcgcacac cctgagcggc gggaaagacc
     2581 gctacacgga ctccgtcaac gtcaacggca aacccgtccg ctaccgcagc aaggacggca
     2641 tacactttac cgccgaagga caaaaactgc tggcggcaaa aataatggaa aaaatcgttt
     2701 ttgaaccaag tacgcaacca tcaagtacac agccatgaat tccaaacacc tcatcgcatt
     2761 ttccgccctg ttcgccgcca cgcaggcaga agccctgccc gtcgcctccg tcagcctcga
     2821 caccgttacc gtttccccgt ccgcccccta caccgataca aacgggctct tgaccgacta
     2881 cggcaatgcc gccgcctcgc cttggatgaa aaaactccaa tccgtcgcac aaggcagcgg
     2941 cgagaccttc cgtatcctgc aaatcggcga ctcgcatacc gccggcgact tctttaccga
     3001 cagcctgcgc aaacgcctgc aaaaaacttg gggcgacggc ggcataggct gggtttaccc
     3061 cgccaacgtc aaagggcagc gcatggcggc cgtccggcac aacggtaact ggcaaagcct
     3121 caccagcagg aacaacaccg gagacttccc gctcggcggc atcctcgccc acaccggcag
     3181 cggcggcagc atgaccctga ccgcatcgga cggcatagca agcaagcagc gcgtttccct
     3241 gtttgccaaa cccctgcttg ccgaacaaac cctgaccgtc aacggcaaca ccgtctccgc
     3301 caacggcggc ggctggcagg tactggatac gggcgcggca ctgcccctga ccatacacac
     3361 cgaaatgccg tgggacatcg gcttcatcaa catcgaaaat cccgccggcg gcattaccgt
     3421 ttccgcgatg ggcatcaacg gcgcacaatt aacccagtgg tcgaaatggc gtgccgaccg
     3481 tatgaacgac cttgcccaaa ccggcgccga tctagtcatc cttgcctacg gtaccaacga
     3541 agccttcggc gacaacatcg acattgccga cactgaacaa aaatggctgg ataccgtccg
     3601 ccaaatccac gacagcctgc ctgccgccgg catcctcatc atcggcgcgc ccgaatccct
     3661 gaaaaacacg ctcggcgtat gcggcacacg ccccgtccgc ctgaccgaag tccaacagat
     3721 gcagcggcgc atcgcccgtc aggggcagac gatgttctgg tcttggcaaa acgcgatggg
     3781 cggcgtttgc agcatgaaaa actggctcaa ccaaggatgg gccgccaaag acggtgtaca
     3841 cttctccgcc aaaggctacc ggcgcgcggc ggaaatgctc gccgacagcc tcgaagaact
     3901 cgtccgcgcc gccgcaatca ggcaataatc ggacaggagg cggacggtat ttccgcaaca
     3961 gggggatgcc gtctgaaacg cataccttca tattgcttca gacggcattc gcctcagaca
     4021 ttggaaagac gcaggcgaac ctccggcata cggcgcaaag gcggcgtttg atatgccgtc
     4081 tgaaggcaaa gatgataaac tgccgccttc cgttttcata cggcatattg ttttcaaatg
     4141 agggcgttcg ccgtccgcaa ccataaagga agtttcatga accggactta tgccaatttc
     4201 tacgaaatgc tcgccgccgc ctgccgcaaa aacggcaacg gcacggcagt gttcgacggc
     4261 aaggaaaaaa ccgcctaccg cgcgctcaag caggaggccg aagccgtcgc ggcgtatctg
     4321 caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg cggtttccaa ttccacagaa
     4381 tttattaccg cctatttcgc cgtctccgcc atcggcgcgg tcgccgtacc gatgaacaca
     4441 tttttgaaaa acagcgaata cgcgtatatc ctgaacgact gcaaggcgcg cttcctgttc
     4501 gcctcggcgg gcctgtcaaa agagttggcg ggcttgaagg cgcaaacgcc cgtcgaaaaa
     4561 atcatttgga cggacaaaag ccgtccggcc ggcgaaatgg cggaaggcga tgcctttttt
     4621 gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc gccaaccccg gataaatgat
     4681 ttggcacaca tcatctacac ctccggcacg acggggcatc ccaaaggcgc gctaatcagt
     4741 tacgccaacc tgttcgccaa cctgaacggc atcgaacgca tctttaaaat ctccaagcgc
     4801 gaccgcttta tcgttttcct gccgatgttc cacagcttca cgctgacggc tatggtgctg
     4861 ctgccgattt atatggcgtg ttcgattatt ttggtcaaat ccgttttccc gttttccaac
     4921 gttttgaaac aggccctgct caaacgcgcg accgtgtttt tgggcatacc cgcgatttac
     4981 accgcaatga gcaaggcgaa aatcccttgg tatttcagat ggttcaaccg cattcgcctg
     5041 tttatcagcg gcggtgcgcc tttggcggaa caaaccatcc tcgatttcaa agccaagttc
     5101 ccccgcgcca aattgctgga aggctacgga ctgagcgaag cctctcccgt cgtcgccgtc
     5161 aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc ccctgcccgg tttggaagcc
     5221 aaagccgtcg atgaagaatt ggtcgaagtg ccgcgcggcg aagtgggcga actgatcgtc
     5281 aggggcggtt cggtgatgcg gggctacctc aatatgcctg ccgccaccga tgaaaccatc
     5341 gtcaacggct ggttgaaaac gggcgatttc gttaccatag acgaagacgg ctttatcttt
     5401 atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc aaaacgtcta tccgcgcgaa
     5461 atcgaagaag aaatctacaa actcgatgcc gtcgaagccg ctgccgtcat cggcgtgaaa
     5521 gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat tgaaggaagg tatggatttg
     5581 ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg caaatttcaa aatccccaaa
     5641 caaatccact ttaaagacgg gctgccgcgc aacgctacgg gcaaggtatt gaaacgggtg
     5701 ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc gtccgaagcc ccgtccggca
     5761 aaaaaaatgc ggtgaatctg cttcaccgca ttttttggca aacgccggag cgaggatatt
     5821 tactctgcca attccacctg ctcgtgcgcc atcagttcct gaccgacatc atccaacaac
     5881 atcaaataac gttcgtagtt tttcaaaatg tcggcaatca gctcgtcctt gttcatatac
     5941 tgcacatcgt acccgacgcg cccgtcgaaa aaataagcgt agggtttgta agttgtctga
     6001 tgccggatat gcggcaggtt gccgtcgtta atcaactggt cggatacatc ctgcccgaca
     6061 gacttaatcc cgtacataaa atcgcgcatc gtctctttcc gaatgacgaa ctcgattgcg
     6121 ggctcgtcct gatgaaacat cttatcgacc cggacgctca agccgtattc ttccgaaagc
     6181 tcccgttgca actcgtgcat agcgggcgat gcggtatgtt tgaggaattt taaaatatcc
     6241 tgctcctgcg tctggctcat tatctgcacc agccgttctt tccacttgcc gcccgtccaa
     6301 aatacactgg tagggttaac ccgggtctca aaatatttct tatccgcact caatcctttc
     6361 cacaggctga aacacattat cagcatcagc agggcaaacg gcagggaaac aatcagggtc
     6421 atagactgca ggttgcctag cccgcccgag cgcatcagca aaacggcaac ggcagacatc
     6481 agcacgcccc acataaccgc ctgccaccgt ggtgcgctca agcccttgtc ccgagaggta
     6541 atattgttca ggacataaat cccggaatcg gcagaagtta caaaaaacag ggaaatgacc
     6601 agcaggctga cgatgctcgt caattcgggc agggggaggt aattaaagaa tttaaaaagc
     6661 agcgtttccg gagaggaggt catcttttcg agcattcccc ccgcaacccc gtcattcagc
     6721 caaatcgccg tattgccgaa gacggtaaac cacaaaacgc cgaacaggcc ggggatgagc
     6781 aaaaccccga agacaaactc gcggatggtg cgcccctttg aaatgcgcgc gataaacaaa
     6841 cccacaaacg gcgcccaaga acaccaccac gcccaataaa gcaccgtcca agattcaaac
     6901 cacggcttgt gttcccgttc gtacgcataa gttttaaaac tgaggcgcac cagattttcg
     6961 aggtagttcc ctatgttgtc gccgaatgcc gacaacaggt aaacagtggg gcccgccgcc
     7021 aaaacaaaaa acagcagcaa aaacgcaagg ctcaggttca actcgctcaa caccttcacg
     7081 cccttcccca cgccggatat tgccgaaacg acggcgaggg acatgacggc ggcgataatc
     7141 aaaacctgca cgctgaagct gttttcggca atccagccca tttcctgcaa tccggcgccc
     7201 agttgcgaag ccccgaaccc caatgtggtg atgatgccga aaaaagtagc aagcaacgcc
     7261 ataatatcaa tggcatcgcc gaaccttccg gaaatttttt ctttcaacag ggggtaaaaa
     7321 caagaacgca gggcaagcgg cagcttgtag cggaaaccga aataagccaa agccaatgca
     7381 atcgtaccgt acaccgacca agcgtgaacg ccccaatgga acaccgtgtg cagcaatgcc
     7441 tgctgctgcc tgtgttccgg cgtgccggcc gtaatgtccg aaaaataatg catcaacggc
     7501 tctgccacgc cgaaaaacat cagacccacg cccatcccgg ccgcaaacag catcgccagc
     7561 cacgacagga agccgaattc cggcacatct tcatcccgtc cgagcctgat gtttcccaaa
     7621 ctgctgaccg agagtatcag caggaaaccc agaaaaatgg aaaacgttaa aacataaaac
     7681 cagctgaact cggtaaaaat gacttctttt gcccgatcga gccacatctg cgcctgatcc
     7741 ggcacggtta aaaccaatac caccaaaaca cacacaaaaa acaaagtcgt caaaataacc
     7801 atcggattaa atgacgttcg gcgttctata aattcagaca gggacaaacc ttctcactcc
     7861 tttgttaaaa acagacaaac ccggtcatcg ggcaaagcgg tcaaaacctg ccggtaaaat
     7921 accggcttcc ggatgacgaa atgcacaaac cggccgaaat tgttataatc gaaaaaatta
     7981 aaaatcaata cggattattc tatcagattg atttatcgat atttattata tcattaatat
     8041 agtttgattt caaaccggcg gaaaatgcgc cgcccaccgc aggcgcgccc tccccgctat
     8101 cggggcgttt aacgccggat tgccgatgcg gtacaatggc tgatatgaag aaaattaccc
     8161 ctcaaaacct gcgccccctg ctttcggaaa gcttgggaca taccgatttt gtcaacgtcc
     8221 tcaacgcact gattaaattt ttgcgccgtg gcggcaaaaa atgtgcgggg gaacgtttcg
     8281 acctgattat cgacacattc aaacaagaca gggaattgct gtcccgcttc agccggtgtt
     8341 tttacatctg gctcgcgcaa atacacattt atccggcact catcaaactc ggcatcttct
     8401 cgcgccacag ctttgcccgg gaaatgggca tacgcatcta cgaacgcttc agcccgtcat
     8461 ataaagattt tgccaacttg ggcgaagtct tcctttatct tttccattcc gaaaacgacg
     8521 acaaatggct gcaaacgctc aatatccgcc aatggctggt tttatacgaa ctcatccgga
     8581 gccacgccga accgtccaaa ttgcagacgg cgggcatccg ccttgccgat gcgcgtttgc
     8641 gcgccatcga aatgctgtct gtctggacgg catccgaagc catcgaaccc gacctcatcc
     8701 gcatcgcccc gcgcctgctg gaagccgatt cttccttcgt cgccctccaa cgcgaaaccg
     8761 ccaaactggt cgaacactac cgcaacggca ccgcgcctta cgacaccgcc cacctcgaag
     8821 tgatgttcga ccaatgtttc agccagattg actatttgcg ccgcaaaggg acgggcgccg
     8881 gctccggttc gtcggtcaaa gtcgcccacc tgctcgaacg gctccggcag accgtagacc
     8941 gtctgaagct gctcaccgac atccaaaccg gcgccggcaa cagcaaccgc ctgaccatcg
     9001 cgctgatgaa ctccctcatc tacgcggcgg tcgaacaata cagcacccgc cacctgcgcc
     9061 gcagcagcat ccgtatgctc gcccgcagca ttaccgaaaa caaaagccac cacggcgaac
     9121 actacatcac ccgcaaccgc aaagaatatt tcaaaatgtt ctactcggcg gcaggcggcg
     9181 gcgtcatcat cgccctgatg gcgctgctca aaatccgcat cggcacactc ggcctcagcc
     9241 ccttcctcac ttccttgtcg gccgggttca actacggcat cggctttatg atcatccata
     9301 tgctgcactg caccgtcgcc accaagcagc ccgcgatgac tgccgccagc tttgccgaac
     9361 aggtcgatct caacgaaggc ggcaaagcgg tggacaacaa actcgccaag ctcctcatcg
     9421 acgtatgccg ctcccaaagc gtcgccgtct tcggcaacgt ttccatcgcc atccttttgg
     9481 cgtgcgccat atcgttcggc tatgcccatc tgtaccggca gcccatactc gatgcccaca
     9541 ccgccgccta ccagttcaaa tccatagaca tcatcgctta cccgacgctg tggtatgccg
     9601 ccattgcagg tctgtggctg ttctgctccg gcatcatcgc aggttttttc gacaaccgcg
     9661 ccgactacct caacctgcgc caacgcctgc ctttcaaccc cttgctgcgt aaaatcatgc
     9721 gccccgggcc ccgccgcgtc ctcgccgcct acatccacaa acactacggc tcgttggtag
     9781 gcaacttcat cttcgggatg ctcttgggta tgaccggcta tttcggacac ctcctcgggc
     9841 tgccgctgga catccgccac gtcgcctttt cctccgccaa cctcggctat gccgccgtca
     9901 gcggcaacgt cggtttgggc acgttcgtac tcggcatttt cagcgtcctc gccatcggcc
     9961 tggtcaacct ctgcgtcagt ttcagcctcg ccctcttcgt cgccctgcgc tcgcgcggca
    10021 cgaaaatcgg cagcatccgc aatctgatta aaagtttttg gaatcagatt aaaagcaatc
    10081 cctgcatact tttcctcccg cccgccaaag aacagggaca tcctccttcg gacaagcctt
    10141 gaccggcaat gccgtctgaa gcgggattcg ccccgaatac cgccctgatg cgggaaatcc
    10201 ccataaaagg atgcaaaaat gccgtctgaa tcgaaacgtg gttcagacgg cattttaaaa
    10261 aacattacaa tcccgggccg ccaagcgaat tggcaatgtc cctgaccgcc gttacataca
    10321 tccgactgtg attgtactgc cataccgtat aaaaattgtt caagcccaaa taatattcaa
    10381 acacgccggg tgcggtttcc agtttgaaca aaaccgcctt ttcatcatcg gcaagctctt
    10441 cgccggggat gatgccgtac gccttcaaat ccgccaccgt ccgcgtcagg gcggtttttt
    10501 cgccaatgat tgcctgaaca tccgcacccg gcgccaatgt tgcagacacc agtattttcc
    10561 cgcccgtgcg ccaaccgtgc tgcttcatat aattggcaac cgatgccgcg acatcgccga
    10621 tgttgcccca tatgtcccga tgtccgtccc cgtcataatc caccgcccat ttccggtagc
    10681 tcgaaggcat aaattgcggc atccccattg cgcccgcata gctgccttta aaggcgaaaa
    10741 catcgccgcc ttcttctttt gccagcttta aaagctcgac caattctttt tggaaaaacc
    10801 cggcgcggcg ggggtaatca aagcctaagg tcgccaatgc gtccgccaca cggaaactgc
    10861 ccgtattttt gccgtaattc gtttcaatcc cgataaccgc cacgataagt tcggcaggca
    10921 cgccgtattt ttgcgccaca tcatcgataa gcgcgcggtt ttccgcataa aaccggcgcg
    10981 cgccgcgaaa tttcgccttg cccgaatttc ccgtgcggaa cacataccac ggacgcgatg
    11041 tggagggtcg gtgcataatc ttgacgatgt ccgccttgta agccgctttg tcaaaaaaat
    11101 cctgccattc cgcccgggaa aaatcccctt tcccgacttc atcgtccaca aaacggcgga
    11161 catttgcatt ggcggcaaac ccgctgtcgg ataccggtac ggctgccgcg tcaaacgcgg
    11221 ggcggctttc ttttttcatt tcatccgcgc ggggggcttg ggcttcattt acccggggcg
    11281 ggcgtgcctc catcgccgta caggcagaca aagccgccaa acaaattgcc agcggcagta
    11341 tttttctctt tttcataaat atgttccgaa caaatagggt aagtgggaaa gcggcgcaag
    11401 gggcgcggtg cgaaatgcgg catccgccgc aatcggcggc tttgtggaat accgcgcgtt
    11461 gccttttgca ccgcctgaaa tccgtatgcc gccgtatttg cccgctatcc gcaatatcgg
    11521 cagtccaata tatctttgcg gatgtcgttc agcaggaagg ctgcggtgtc ttcgtgttcc
    11581 gcctgtacga agacaaagag tcgggcgatg attttgccga tgaagacttt ttgcagggcg
    11641 caggcgtcgg tcagggtgtg ttcctgcagg aagctgcgga tgtcttcggg caaagtgtag
    11701 tcgcgggcga cggcggcaaa gcgggcatcg gtttgcaggc ggtcgagcag ggtttggttt
    11761 ttgtccaact tcatgcctgc ttctttttct tcggcggtgg cgcggacgaa ctggtcgtaa
    11821 atttcggcat aaagcatatc gttcgggcct tcaaaaatcg tgaagggacg gatgtcgata
    11881 gcgatattgc cggcggtgtg tccgcgttca aaacccttcg cgcccaaaag tttttgcaac
    11941 atttgcgcgg cggcgtaagt gtattccgtg gcgagggttt tgacgatgtt cgcctccatc
    12001 agctgatggg cgacgggtgc aacgggcgaa acggaatggc agacgtagcg gtaaagaatc
    12061 tcggaaacct gatggcggcg ccggatttcg cggcgttcgt aatcgacgaa tctgatgtcg
    12121 ttgcggacgt atcgttccag gttttcaagg atgtattcca taatgccgtg cgtcatgccg
    12181 atcagttgca ggcggctgcg gataaagatg ttttggaacg cgcgcaaacc tgcggcgtcg
    12241 ctctgggaga gtttcatcac ggcggttgca ggcatttcgg catcgatgcg gttgacggcg
    12301 taacggacgg cgcgcaagcc ttcggatgcg agggtttcgc agcggatgta tgttttgggg
    12361 acgagcagca ggtcgatgac tttggcgagt ttgccgtttt tgcgttcttt ggcggcaacg
    12421 aggaggaagt cgctttgcga gttgccctgc cagtattttg cggcgttgac gtaaatggtt
    12481 tgtccgtcgg tatattcgta gtaagactgc atttctcggg caatcgccgc gccggaggtt
    12541 tcgggttcgg taacgcccaa accgccgccc tcgcctttga aaaccatgtc caaaccctgt
    12601 gcgatttgcg cttcatcgcc gaactcttgc agtggctgca acaccagcgc gccttcgatg
    12661 ccggtacgca gcgtaacggg tacgccgtaa tgccccgcaa tccgcaatac ttcctgaatt
    12721 tcaaactggc tgcccttgcg gccgccgtgt cttttgtcga ggaagggcaa cagcaaaccc
    12781 gcctgcttca aggcaagcca tttgtcttcg ggcaggtatc gcatcaggtc gataccgtct
    12841 gaaaaaatgc ggcggaatgc ggattcgatg tgctttaaaa aagcagccgt gtccatagtt
    12901 gacggctgcg cgctcggttc ggtgtgtatc atcggcttcc tctgtcggtt cccattaatc
    12961 ggcggccggt caaaccgcct gccacagttt agagttgatt ttctaaactt taccacaaag
    13021 tgcgccgggc aacaatccgc cggcttttca gacggcatcg cgcaccctcc cgtgctaaaa
    13081 tgaccgtttg catcactgtc cgccgattgc cgcactatga cctaccccat ccccaaaccc
    13141 cgtgaaaaat cccgttggcc caatctttcg caaggctcgc tgcccttggc tttggcgcgt
    13201 tatctgccgc acaagcggct caaggtcgtg ctgacccaag atgcggaaca ggcgttgcgc
    13261 cttcagacgg catggcggtt tttccgtccg cacgacacgg cggtgttctt gccggactgg
    13321 gaaacgctgc cttacgagcg tttttctccg catcaggatt tggtgtcgga gcggctctcg
    13381 gcgttgtggc agattaagag cggcgcggcg gacgtgttgt ttgtgccggt tgccacggcg
    13441 atgcagaagc tgccgcccgt gccgtttttg gcggggcgca cgttttggct gaaaacagga
    13501 cagactttgg atataggccg tctgaaaacc gatttggtag atgcgggcta caaccatgtt
    13561 tcccatgttg ttgcggcggg cgagtttgcc gtgcgcggcg gcattgtcga tttgttcccc
    13621 atgggcagcg aaatgcccta ccgcatcgat ttgtttgacg atgaaatcga cagcatcaaa
    13681 acctttgata cggacacgca gcgcaccatc tcccccgttt ccgaaatccg cctgctgccg
    13741 gcgcacgaat tccccaccga cagcgaggcg caaaaaatct tccgcagccg cttccgcgag
    13801 gaagtcgatg gtaatccgaa cgatgcggct gtatacaaag cagtaagcaa cggccacttc
    13861 ggtgcgggcg tggaatatta cctgccgctg ttttttgaaa acgagctgga aacgctgttt
    13921 gactatatcg gcgaagacgc gctgtttgtc tctttgggcg atgttcacgc cgaggcaaac
    13981 cgcttttgga gcgacgtcaa atcgcgttac gccatggcgc agggcgacga aacctatccg
    14041 cctttgcttc cacagcattt gtatctctct gccgatgttt ttgcaggccg tctgaaaaac
    14101 tacggacagg tgctgcccga tgtttccggc aaggaacacg ccctccccga ccttgccgtc
    14161 aaccgacaat ccgacgagcc tttgcaggca ttgaaggatt ttcagacggc gtttgaagga
    14221 cggattttgc tgtgcgccga aagtttggga cggcgcgaaa ccatgctcgg cttcttgcag
    14281 caaaacggtt taaaagccaa aagcgtgtct gattggcaag gcttcttgtc ggcacatgag
    14341 ccgctgatga ttacagtggc accgttggct tacggattca aactgggcgg attgcaatcc
    14401 ccgaaccagc agcaaactac ttccgcctcc gaaggagaag gcgatgcggt taccgaccaa
    14461 accgaatttt ccgcatccgc aacaaaccct ctccctagcc ctctcccaca ggagagggaa
    14521 caaagtgcag ccgccgtttc agacagtctg aaagcagccg ccgtttcaac cgaaagcagc
    14581 ctgcccctcg gtacaagtaa tctgcacggg caaatccgac agcaacctgc cccttccccc
    14641 gtgggggagg gttggggaga gggcaaagca gttgccgctc aaaccgaatt tcccgcatcc
    14701 gcaacaaacc ctctccctag ccctctccca caggagaggg aacaaagtgc agccgccgtt
    14761 tcagacgacc tgaaaaccaa aagcagcctg catcccgtcg caaataatct gcacgggcaa
    14821 atccgacagc aacctactcc ctcccccgtg ggggagggtt ggggagaggg caaagcagtt
    14881 gccgctcaaa ccgaattttc cgcagccgca acaaaacctc tccctagccc tctcccacag
    14941 gagagggaac aaagtgcagc cgtcgtttca gacagtctga aagcagccgc cgtttcaacc
    15001 gaaagcagcc tgccccccgg taaaagtaat ctgcacgggc aaatccaaca gcaacctgcc
    15061 ccctcccccg tgggggaggg ttggggagag ggcaaagcag ttgccgctca aagtgccatc
    15121 gccgtcatca ccgaatccga tctctaccaa tacgtcgccc gttcgcgcgt ccacaaccgc
    15181 cgcaagaaac acgccgccgt ttcagacggt ctgttgcgcg accttgccga aatcaacatc
    15241 ggcgaccccg tcgttcacga agaacacggc atcgggcggt atatgggctt gatcacaatg
    15301 gatttgggcg gcgaaaccaa cgaaatgatg ttgcttgaat atgcaggcga agcgcagctt
    15361 tatgtgcctg tttcgcaact gcatttaatc agccgctact ccggtcaggc gcatgaaaac
    15421 gtcgccctgc acaagctcgg cagcggcgcg tggaacaaag cgaaacgcaa agccgccgaa
    15481 aaagcgcgcg ataccgccgc cgagttgctc aacctctacg cccaacgcgc tgcccaatcg
    15541 ggacacaagt ttgaaatcaa cgagttggac tatcaggcgt ttgccgacgg tttcggctac
    15601 gaggaaaccg aagaccaggc cgccgccatc gccgccgtga ttaaagacct gacacaggcg
    15661 aagccgatgg accgactcgt gtgcggcgat gtcggctttg gcaaaaccga agtcgccctg
    15721 cgcgccgcgt ttgtggcggt gatgggcggc aaacaggtcg ccgtactcgc cccaaccacg
    15781 cttctagtcg agcagcacgc gcaaaacttt gccgaccgtt tcgccgattt ccccgtgaaa
    15841 gtcgccagcc tttcgcgttt caacaacagc aaagccacca aagccgcact ggaaggcttg
    15901 gcggacggca cggtcgacat cgttatcggc acgcacaaat tggtgcagga caacatcaga
    15961 ttcaaaagct taggtttagt gattatcgat gaagaacacc gcttcggcgt gcgccagaaa
    16021 gaacagctca aacgcctgcg cgccaatgtc gatatcctta ccatgaccgc cacgccgatt
    16081 ccgcgcaccc tcagcatggc actcgaaggc ctgcgcgact tctcgctgat taccaccgcg
    16141 cccagccgcc gcctcgccgt gaaaaccttt gtcaaaccgt tcagcgaagg cagcgtgcgc
    16201 gaagccgtgt tgcgcgaact caaacgcgga ggacaggtat ttttcctgca caatgaagta
    16261 gatacgattg aaaatatgcg cgagcggctg gaaaccctgc tgcccgaagc ccgcatcggc
    16321 gtggcgcacg gacaactgcg cgagcgcgag ctggaacaag tcatgcgcga ctttttacag
    16381 caacgattca acgtgttgct ctgttccacc atcatcgaaa ccggtatcga cattcccaac
    16441 gccaacacca tcatcatcaa ccgcgccgac aaattcggac tggcgcaact gcaccagctt
    16501 cgcgggcgcg tcggacgcag ccaccaccaa gcctacgcct acctgctcac gcccgaatac
    16561 atcaccaaag acgcagaaaa acgcctcgac gccatcgcgg cagcagacga actcggcgca
    16621 ggtttcaccc tcgccatgca ggatttggaa atccgcggcg caggcgaaat cctcggcgaa
    16681 gggcagtccg gcgaaatgat acaggtcggc ttcacgctct acaccgaaat gctcaaacaa
    16741 gccgtgcgcg atctcaaaaa aggccgccaa cccgacctcg acgcgccgct gggcatcacc
    16801 accgaaatca aactgcacag ccccgcccta ctgcccgaaa gctactgccc cgacatccac
    16861 gagcgtctcg tcctctacaa acgcctcgcc gtctgcgaaa ccgtgcagca aatcaacgcc
    16921 atacacgaag aactcgtcga ccgcttcggc ctgcccgaac aacccgtcaa aacccttatc
    16981 gaaagccacc acctgcggct tatggcaaaa gaattgggta tcgatgccat tgatgcggcc
    17041 ggcgaagcgg taacggtaac cttcggtaaa aacaataatg tcgatccaac cgaaatcatc
    17101 ctgctgattc agaacgacaa aaaataccgc cttgccggcg ccgataagct gcggtttacc
    17161 gcagagatgg aaaatatcga ggtcagaatc aacaccgtaa aaaacgtttt aaaaaccttg
    17221 caaaacagat gcctgcccaa ataaagccga caccgcaatg ccgtctgaaa caccgttttc
    17281 cttgtccgaa agccgccatt atgaatttga aggaaactcc actataatac ggcattcaga
    17341 tctccagacg gcatcgcgcc cgtcagaccg cacacaaacc aaaaggaaat acatgttccg
    17401 tactatgctt ggcggaaaaa tccaccgcgc caccgttacc gaagccgatt tgaactacgt
    17461 cggcagtatt accgtcgatc aagacctgtt agacgcagca ggcatctacc ccaacgaaaa
    17521 agtcgccatc gtcaacaaca acaacggcga acgttttgaa acctatacca tttcaggcaa
    17581 acgcggcagc ggcgtggtct gcctgaacgg ggcggcggcc aggctggtac agaaaggcga
    17641 tatcgtcatc atcatgtctt acgtccaact ctccgaaccc gaaatcgccg cacacgaacc
    17701 caaagtcgtc ttggtagacg gaaacaacaa aatccgcgac atcatctcct acgagccgcc
    17761 gcacaccgtg ctgtaaccct gaaaacggac attgaccatg gatattaaaa tcaacggcat
    17821 taccgtcggt aacgatgctc ctttcgtact gttcggcggc atcaacgttt tagaagattt
    17881 ggattccacc ctccaaacct gtgcgcatta cgtcgaagtt acccgaaaac tcggtattcc
    17941 ctatatcttt aaagcctctt tcgacaaggc aaaccgttcc tccatccatt cttatcgcgg
    18001 cgtaggcttg gaagaaggct taaagatttt tgaaaaagtc aaagcagagt tcggcatccc
    18061 cgtcattacc gacgtacacg aaccccatca gtgccaaccc gtcgccgaag tgtgcgatgt
    18121 catccagctt cccgcctttc ttgcgcggca gaccgatttg gtggccgcaa tggcggaaac
    18181 gggcaatgtc atcaacatca aaaaacccca gttcctcagc ccttcgcaaa tgaaaaacat
    18241 cgtggaaaaa ttccgcgaag ccggcaacgg gaaactgatt ttatgcgaac gcggcagcag
    18301 cttcggctac gacaacctcg ttgtcgatat gctcggtttc ggcgtgatga aacaaacctg
    18361 cggcaacctg ccggttattt tcgacgttac ccattccctg caaacccgcg atgccggttc
    18421 tgccgcatcc ggcggtcgtc gcgcacaggc tttggatttg gcacttgcag gcatggcaac
    18481 ccgccttgcc ggcctgttcc tcgaatcgca ccccgatccg aaactggcaa aatgcgacgg
    18541 ccccagcgcg ctgccgctgc accttttaga agatttttta atccgcatca aagcattgga
    18601 cgatttaatc aaatcacaac cgattttaac aatcgagtaa cacggtttcg ccttatgatg
    18661 cagactttcc gaaaaatcag cctgtatgcc gcaaccttgt ggctcggtat gcagattatg
    18721 gcgggttata tcgtcgcgcc ggtgctgttc aaaatgctgc ccaaaatgca ggcgggcgaa
    18781 attgccagcg tattgttcga catcctctct tggagcgggc ttgccgtttg gggcgcggta
    18841 ctggctgccg cctttgccgc cctaacccgg cggcaaaccg ccctgctgct ttttttattg
    18901 tccgcccttg ccgccaaccg attcttgatt acacccgtta tcgaggcgct gaaatacggg
    18961 catgagaatt ggctgttgtc gtttgtaggc ggatccttcg gaatgtggca cggcatttcc
    19021 agtattgttt ttatggcaac cgccctactt tcagcagttt taagttggcg gctttccggc
    19081 aaagaggccg tctgaagccc tcccattttt tacctccctt cacttcactt ggagaacatt
    19141 catgagcgca atcgttgata ttttcgcccg cgaaattttg gactcacgcg gcaaccccac
    19201 agtcgagtgt gatgtattgc tcgaatctgg cgtaatggga cgtgcagccg taccgagcgg
    19261 tgcgtccacc ggtcaaaaag aggctttgga actgcgcgac ggcgacaaat cccgttattc
    19321 gggcaagggc gtattgaagg cggtcgaaca cgtcaacaac caaatcgccc aagccctcat
    19381 tggtatcgat gccagcgaac aatcttatat cgaccaaatc atgatcgaat tggacggtac
    19441 tgaaaacaaa ggtaacttgg gtgccaacgc gactttggcc gtttctatgg ccgtagcacg
    19501 cgcagccgct gaagatgcag ggctgccgct ttaccgctac ttgggcggcg caggtccgat
    19561 gtccctgccc gtaccgatga tgaacgtcat caacggcggc gaacacgcca acaacagcct
    19621 gaacatccaa gagtttatga ttatgcccgt cggcgcaaaa tctttccgcg aagcgttgcg
    19681 ctgcggtgcg gaaattttcc acgccttgaa aaaactgtgc gacagcaaag gcttcccgac
    19741 cacagtcggc gacgaaggcg gtttcgcccc caacctgaac agccacaaag aagccctgca
    19801 actgatggtc gaggcgaccg aagccgccgg ctacaaagcg ggcgaagacg tattattcgc
    19861 attggactgc gcctccagcg agttctacaa agacggcaaa taccacttgg aagccgaagg
    19921 ccgctcctac accaacgcgg aatttgccga atatctggaa agtctggtca acgagttccc
    19981 catcatctcc atcgaagacg gcatggatga aaacgactgg gaaggctgga aactgctgac
    20041 cgaaaaactg ggcggcaaag tccaactcgt tggcgacgac ctcttcgtta ccaacccgaa
    20101 aatccttgcc gaaggcattg aaaaaggcgt ggcaaacgca ctattggtca aagtcaacca
    20161 aatcggtact ttgagtgaaa ccctgaaagc cgtcgactta gccaaacgca accgctacgc
    20221 cagcgtaatg agccaccgct ccggcgaaac cgaagacagc accattgccg acttggcagt
    20281 cgccaccaac tgtatgcaga tcaaaaccgg ttctttgagc cgttccgacc gcatggcgaa
    20341 atacaaccaa ctgctgcgta tcgaggaaga attggcggaa gccgccgact accccggcaa
    20401 agccgcattc taccaactgg gcaaataaaa aaggttaagg tatgaagtgg gtaactgtcg
    20461 ttttatcctt cgcacttgtc tgttgccaat acagcctctg gttcggcaaa ggcagcatcg
    20521 gacgcaacag cagtctgaga gaacagattg ccgttcaaga agaaaaaaac cagacactcg
    20581 ccctacgcaa tcattccctt gccgccgaag tctatgattt ggaaaacggt caagaagcca
    20641 tttcggaaat cgcccgggta gaactgggtt atatccaaga cggtgaaacc ttttaccgac
    20701 tcatcaggca taaccggtaa taccgtcaaa aagccgtccg aaccaatgtt cggacggctt
    20761 ttatttcaac aaactgtcag acagcccctc atcctccccc gacaaaccgc aatccagcct
    20821 gacatccccc tcgacgtaac agcagcacgg cagtatctcg tcccgcccca aaaaagccaa
    20881 aggcggcttc cgataagtaa cgcttccctc caaaatcttc acccggcacg atccgcaata
    20941 tccgcttcgg cactgatatt ccaccatatg ccccgtccgt tccaagcctt ccaacagggt
    21001 ctcgccctcc aagagctcaa aaaaaccctt attcgtacca atgcgcgcca tttccgacca
    21061 atca
//
LOCUS       DAWFAD010000018        20934 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWFAD010000018 DAWFAD010000000
VERSION     DAWFAD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20934
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.018"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(79..771)
                     /locus_tag="WGO49_000502"
     CDS             complement(79..771)
                     /locus_tag="WGO49_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0305655.1"
                     /translation="MFHAFILRIYTMIKNISKLINHAVLIGFIPNIVFAYPIFAENPA
                     SYQNIHVRSWKARRDSGIVKQDLDFSCGAASIATLLNNFYGRHYSEAEILDKMDKTQM
                     RASFDDMQRIMPELGFEAQGYALPFEQLVQLKIPVIVYLKYRKNNHFSVLNGINGETV
                     LLADPSLGHVSMSKSQFLSAWKTRDGEMEGKILAIVPKNTDFVRNQMFFNKNPVRQTR
                     FTVEQIQMRQKR"
     gene            complement(1077..1634)
                     /locus_tag="WGO49_000503"
     CDS             complement(1077..1634)
                     /locus_tag="WGO49_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305656.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLEIWHGDRVFMH"
     gene            complement(1879..2037)
                     /locus_tag="WGO49_000504"
     CDS             complement(1879..2037)
                     /locus_tag="WGO49_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305657.1"
                     /translation="MKPPLPICTLSALAACTLSGQGGTQVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2034..2876)
                     /locus_tag="WGO49_000505"
     CDS             complement(2034..2876)
                     /locus_tag="WGO49_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0305658.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3115..4035
                     /gene="pyrB"
                     /locus_tag="WGO49_000506"
     CDS             3115..4035
                     /gene="pyrB"
                     /locus_tag="WGO49_000506"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ0305659.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4045..4503
                     /locus_tag="WGO49_000507"
     CDS             4045..4503
                     /locus_tag="WGO49_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0305660.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4568..5233
                     /locus_tag="WGO49_000508"
     CDS             4568..5233
                     /locus_tag="WGO49_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305661.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIPPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALDLGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5296..6513)
                     /locus_tag="WGO49_000509"
     CDS             complement(5296..6513)
                     /locus_tag="WGO49_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0305662.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFDRSE"
     gene            6655..7200
                     /gene="def"
                     /locus_tag="WGO49_000510"
     CDS             6655..7200
                     /gene="def"
                     /locus_tag="WGO49_000510"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ0305663.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            7287..8213
                     /locus_tag="WGO49_000511"
     CDS             7287..8213
                     /locus_tag="WGO49_000511"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ0305664.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            8292..9551
                     /gene="rsmB"
                     /locus_tag="WGO49_000512"
     CDS             8292..9551
                     /gene="rsmB"
                     /locus_tag="WGO49_000512"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ0305665.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAILRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIPARALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9517..10116
                     /locus_tag="WGO49_000513"
     CDS             9517..10116
                     /locus_tag="WGO49_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ0305666.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10116..12233
                     /locus_tag="WGO49_000514"
     CDS             10116..12233
                     /locus_tag="WGO49_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ0305667.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLISTASLSGNMGRVLEHY
                     ANGFTQLSLYNAASGKIEKSINPHKLDQPFPDKARWEKIQQAGSVRDLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRQREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAEQ
                     ILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGQDGRIVLTVCDNGKGFGKEMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVKTYA"
     gene            12226..13503
                     /locus_tag="WGO49_000515"
     CDS             12226..13503
                     /locus_tag="WGO49_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ0305668.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QVRPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSAVENALKHGAAKIETGPVFDKLGNSAAIQEMNCEVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQTGIAFIIGKAERCRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13594..14787
                     /gene="dprA"
                     /locus_tag="WGO49_000516"
     CDS             13594..14787
                     /gene="dprA"
                     /locus_tag="WGO49_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ0305669.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     ALAVRHKQAAEAWRNAEKRASARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRALGGKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAERGLIVSEFPLDTRPFAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLTECLDDILNECPRLLQNEGASSYSINKGMPEKRITAVQTASDQLSLPEGKMPS
                     EKTENRPVGGSILDRMGFDPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRY
                     QRIRT"
     gene            14813..15274
                     /locus_tag="WGO49_000517"
     CDS             14813..15274
                     /locus_tag="WGO49_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ0305670.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15346..17652
                     /gene="topA"
                     /locus_tag="WGO49_000518"
     CDS             15346..17652
                     /gene="topA"
                     /locus_tag="WGO49_000518"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ0305671.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAESCPKCAWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17725..18420)
                     /locus_tag="WGO49_000519"
     CDS             complement(17725..18420)
                     /locus_tag="WGO49_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305672.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFAPEMN
                     RILSDFKTTRGFK"
     gene            19111..19488
                     /locus_tag="WGO49_000520"
     CDS             19111..19488
                     /locus_tag="WGO49_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0305673.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNV
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19577..20146
                     /gene="rsmD"
                     /locus_tag="WGO49_000521"
     CDS             19577..20146
                     /gene="rsmD"
                     /locus_tag="WGO49_000521"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ0305674.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVCSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PDIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20269..20520
                     /locus_tag="WGO49_000522"
     CDS             20269..20520
                     /locus_tag="WGO49_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ0305675.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            20617..20700
                     /locus_tag="WGO49_000523"
     tRNA            20617..20700
                     /locus_tag="WGO49_000523"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20651..20653,aa:Tyr,seq:gta)
     gene            20731..20804
                     /locus_tag="WGO49_000524"
     tRNA            20731..20804
                     /locus_tag="WGO49_000524"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20763..20765,aa:Gly,seq:tcc)
     gene            20814..20888
                     /locus_tag="WGO49_000525"
     tRNA            20814..20888
                     /locus_tag="WGO49_000525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20846..20848,aa:Thr,seq:ggt)
BASE COUNT         5260 a         5299 c         5683 g         4692 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat ataaaataaa
       61 tgggcaaaaa tcggtttatt atcgtttttg ccgcatttgg atttgttcta ccgtaaaacg
      121 tgtttgacga acgggattct tattaaaaaa catctgattt ctaacaaaat cagtattttt
      181 tggcacgatg gctaaaattt ttccttccat ttcgccatca cgtgttttcc atgcgctcaa
      241 gaattgtgat ttgctcattg agacgtgccc cagcgatgga tcagccagca aaacagtttc
      301 tccgttaata ccgttcaata ccgaaaaatg gttgttttta cggtatttta aatacacaat
      361 tacaggaatt tttagttgta ccaactgttc aaatggcaaa gcataacctt gtgcttcaaa
      421 acccagttcg ggcattatgc gttgcatatc atcaaaagaa gcacgcattt gggttttatc
      481 cattttgtcc aagatttccg cttcagaata atgtctgcca taaaaattat tcagtaacgt
      541 ggcaatcgaa gccgcgccgc aagaaaaatc caaatcttgt tttactatgc cggaatctcg
      601 ccgtgctttc caactccgta catggatgtt ttggtaagaa gcggggtttt cagcaaatat
      661 agggtaagca aaaacaatat ttgggataaa gccaattaaa accgcatggt ttattaattt
      721 agaaatattt tttatcatag tatatatgcg tagaataaac gcatgaaaca taaaaaaata
      781 aaatcatatg caattttatt gcatgaataa atatgaataa aatagataat gatgggagta
      841 aatacgccat gtattttgga agtttaaatt tattaataat aaaataattt atcgtagcgc
      901 aaataaatcc caaaattgga acgattagaa aaaaaattaa tacacccatc atcatgcaaa
      961 ctctatatta ataaatagct aactaattct attgtggaaa ttagttagct aactaaaagt
     1021 tattaatgat tatttccgag aattgactgc attgttggca gcattggccc caaaacctag
     1081 tgcatgaata cccggtctcc atgccaaatt tccagccaat ccgcctccgg cagcagcagc
     1141 cagccctgtt ttgccgctac tcctgttgcc gcaccgattc ctgccgcagt agccgcgcct
     1201 tgcgcagttc ctaatttacc atgattatac aaattagcac catgatactc ccatgcacct
     1261 aatgcaccgc caaaagcagc ggctgcaata atgggaacaa attcaccttg tgtttctttc
     1321 atttcagcct gtgataattg aattgctttc acattttggc tgtcaaaaac ttggctgtct
     1381 aaattttgcg ccattacagg tgtaatcatc atagccatta cagttgcaat tttcgttgcg
     1441 ctggtttgca cataaatagg attagcaaat tcgctttgat tgcgttcagt gttgatgtag
     1501 ctaatactgc tttctagttt gaatttaccc ttgtcagtca ataattcttc caaacttaaa
     1561 ggtaagtcag cataagcaat ttggctggca acagtcaaaa taaaatctat tagacatttg
     1621 tgtttttgca tcatttcgtt tgattttcta gtttttgaga atgatacaaa gtttttttac
     1681 aaagtaaaga gtcactctga aaaacatttt ttcattataa atcaaaatat tgatagaata
     1741 aatagcgagc atcgattcac ggtgtacttt agtgcaaagc gtacaacgcg agcctgaacc
     1801 accagccgca acaggaaaag aaagccgata gagtgcaatg cttgccaacg tgcaagcgag
     1861 cttgcaagaa cgcttggctc aacgagagca ggcaagacag aaagcagaaa gcaggatagg
     1921 agcggtaacg caaaggtctc gggctttgat ttcgccgtaa acctgagtgc cgccttgtcc
     1981 ggaaagggtg caggcggcga gtgccgacag ggtgcagatg gggagggggg gtttcatttg
     2041 gggtcgcaac ggaagtggta tgcgcagatt tcaaaaccgt ttttgaaata caggcggtgc
     2101 gcgtcggcac ggtcgtggtt gacgtggacg ttgaggtgga ttttggttac ccctgtttcc
     2161 gcgccgattt tgcggacttc ttccaaaagg cgcgaggcgt agcctttgcg gcggctttgc
     2221 ggcagggtaa cgatgtcatc gatgtggatg tggcggccgc tggcgagggt gcaggcttcg
     2281 cggaagccgc agacggcgac ggcattgtgt ttgccttctt caaaaatacc cagcaggcgg
     2341 tagccttggg ggcgttggac tttgttgatc tgttcggtaa agcggttgat gtcggtcagg
     2401 gcggaacgca aaacgctcaa ggctgcaaag gcggtggcgg tgtcgtccgc gccgatttcg
     2461 cgcaaaacgt aggatgcgcc cgaggcggtc tgttcctgtg ctttctcggc ggcgtgtttt
     2521 tcttcgattg cctgtgccag catgacgtgt tcgtcggcag ggttgttttg tccgccctgt
     2581 tcgcgttctt cgagcagggc tttgcagtcg atgacgcgca ggtcgttgtc ggcggcaaag
     2641 tccatcagga agcggaacat ttgggggttg tcggtttcca gttttttatc gacggcgacg
     2701 cagcggatgt tgtccaccag catggggcgc acccatttcg aataggaaag cctgtggtct
     2761 ttggtgaacg aggacagaat gccgcacagg cgttccgccc agtcgctggg acggaaaatc
     2821 ttgccggaac tcgttgtgcc gtggatgacg acttcgtagg ggttgcagac taacatggcg
     2881 gcttcctgaa aagaaatgtc tggcgcgatt ataccttatg cttatgcggg cgtgtttgga
     2941 tatgccgtct gaaaagtacg ggattcgtgc ggtaaaactt tgcggcggca aatgtgcgat
     3001 aatacgcgcc gtattgccgc ttttgcgaag ctgttccgca aacatacggg cggcgtggac
     3061 gacgtataac cggatacccg cctgacgcgg gttttttacg gaaggggggc aaaaatgcct
     3121 aatccgcttt acagacagca tatcatctcc atttcggatt tgtcgcgcga acagttggaa
     3181 tgcctgcttc agacggcatt gaagctgaag gcgcatccgc gcggcgattt gttggaaggc
     3241 aaacttatcg gttcgtgctt ctttgaaccg tccacgcgca cgaggctgtc gtttgaaacg
     3301 gcggtgcagc ggctgggcgg caaggtcatc ggcttttcgg acggcgcgaa taccagtgcc
     3361 aaaaaaggcg agacgcttgc cgataccgcc cgcatcattt ccggctacac cgacgcaatc
     3421 atccaacgcc accccaaaga cggcgcggcg cgcgtggcag cggagttttc gcgcgtcccc
     3481 gtcatcaacg ccggcgacgg caccaaccag caccccagcc agaccttgtt ggatctggtt
     3541 accatttatg aaacgcaggg acgtttggac aagctcaaaa tcgccatggc gggcgacttg
     3601 aaatacggac gtaccgtgca ttcgctttgt caggcgttga aacgctgggg ctgcgaattt
     3661 gcctttgttt cgccgcccag cctcgcgatg cccgactaca ttaccgaaga gttggacgaa
     3721 gccggctgcc gataccgtat cctcggtagt ttggaagaag cggcggaatg ggcggacatc
     3781 ctgtatatga cccgtgttca acgcgaacgt ttcgacgaac aggaatttgc caaaatccaa
     3841 ggcaaattca atctcgacgc atccatgctt gcccgcgcca aaccgaacct gcgcgtgctg
     3901 caccctttgc cgcgcgtgga cgaaatccat cccgatgtcg atgctacgcc gcatgcctat
     3961 tatttcgagc aggcgaccaa cggcgtttat gcgcgtatgg cgatattgtc gctggtgttg
     4021 aacgaagaag tgtgaggaac cgatatggaa accccgaaac tcagtgtcga agccattgaa
     4081 aaaggtacgg ttatcgacca tattcccgcc ggcagggggc tgaccatcct gcgccagttc
     4141 aaacttttgc actacggcaa cgcggtaacc gtgggcttca acctgcccag caaaacccaa
     4201 ggcagcaaag acatcatcaa aatcaaaggc gtgtgcttgg acgacaaagc cgccgaccgc
     4261 ctcgccctgt tcgcccccga agcggtggtc aataccatcg acaatttcaa ggtcgtgcag
     4321 aagcggcatt tgaacctgcc cgatgaaatt gccgaagtgt tccgttgccc gaacacgaat
     4381 tgcgccggcc acggcgagcc ggtcaaaagc cggttttatg ttaaaaaaca caacgggcag
     4441 acgcggctga aatgccatta ctgcgaaaaa acctacagcc gggattcggt ggcggaagcc
     4501 tgacggattc ccttaaaccg agcgggcggc atttcgtctg ccgcttgttt tgccaatctg
     4561 aaatggaatg atgatgcacg cttctgtcca aagccgtttc gcaccgatac tttatgtttt
     4621 gattttcttt gccggttttt tgaccgcgca aatttggttc aatcagaaag cctatactga
     4681 agagctgcct ccgcttctgt ccgcattgtc cgccgtcgcg ctggtgtggc tggcgtgggc
     4741 gttcgtgtcg gcgcgttcaa aggctaaggc ggaaaagttc taccgcgaaa aaatgataca
     4801 gaacgaaagc ataccccccg tcctgcacgc ttctttgcaa cacttggaac acaagccgca
     4861 aatgctcgcc ctgctggtca aaaaccacgg caaagggatg gcggaacagg tcaggttcaa
     4921 ggcggaagtg ctgcccgacg acgaagacgc gcgcacgatt gccgccgagt tggcaaaaat
     4981 ggatatgttt gcattgggga cggacgcggt cgcctcgggc gaaacctatg gacgcgtgtt
     5041 cgccgatatt ttcgagttgt cggcggcttt ggaagggcgc gcgttcaaag gaatgttgaa
     5101 actgacggcg gaatataaaa acatcttcgg cgatgcctgc cgttcggaaa cggcgttgga
     5161 tttgggcgcg ctcaatcagg cgttgcagga gatttcaaaa acatcggaaa agtccaaacg
     5221 gatattttat tgaagatgga aaaatgccgt ctgaaacgga aggtgtttca gacggcattt
     5281 ttgtcggatg attaattatt cggagcggtc gaagccaaac ttcacgcggc tgcggccctg
     5341 atccggtata ttgtccaaat cgcgtcccgg attggcggcg gtgtcgccta cggaaatatc
     5401 ggagatgttt tccaaaatga tggcggacga caggtgttcg gaggtgcggt aaaccattgc
     5461 caagcccact tcttcggcag gagtggaaat cagctcgacg gtatccctgc ttttgaaatt
     5521 gttggagagg tcgacctgca tcgttttctt gcgtttgtag aggctcaaaa ccgtgccttt
     5581 gtccaaaccg tccgcctcgc ctttgtcgat ggtgatggtt tgaaactggc cggcaatcct
     5641 tgtaccttca aacacggaaa cgattttagc ctgaaccggg cgggacggtt cgtgcggcat
     5701 catgttgaag cggtcggtgt cttccggcat tttcatcagg tagtcgccct gctgtatttc
     5761 ggaaatggcg gtttcgacca ccagcggctg tatcgaaggg gtgcgcagcg gggtaatcaa
     5821 aggatgggtg cgggtatggt attcgttgtc tttcggccgt tctccagcct gtttcgagcg
     5881 ttgttcgagg acagagtcgg tatagtcgag ggagcgcacg atgccgctga atgcgacttc
     5941 ctgcccgagg aatttacccg tatccggatc ggtgatgttt ttattgattc ggtaggtcag
     6001 gtagcggccc ggctctttca ggcctttggt gtaaaccctg gtgcctttgg tgtacagcag
     6061 cctgccttcc gggcccgaga gcaggcgcgg cgcggcagcg gtttctttgc gggaaacgat
     6121 ttgcgggtgc cgcataaaga tgcggtagaa gttgacatcg atggcgggaa taccgtatcc
     6181 ggacacttcc ttatccggac tcattttgac gacggggatg ccgtctgtct gttccaagcc
     6241 gaggcgcggt tcgccgtcaa cgtggcgcaa caccaatacc tggtccggat aaatcaggtc
     6301 gggattgtgg atttgatccc ggttcgcgtc ccacaggcgg ccccattgcc acgggctgta
     6361 caggtatttg cccgaaatgc cccacagggt gtcgccctgt ttgaccgtgt agcgttccgg
     6421 cgcgttcggg cgcacctcca aatttgccgc caaagtttgt gttgagaatg ccatacctgc
     6481 cgcgcagagc agggttataa tacgacgttg cataaccgtt ccccttatct gataaatttc
     6541 ggtttgtctt gcttgattgg gttggaaaaa gcggcggcag cccctcggga tgtgccgcgt
     6601 gataaaaaat gttccgcatt ttaacatcga attatccgca ccatcacggt aattatgaaa
     6661 aacaggcggc gtatccgccg aaggaaagag aaaattatgg ctttattgaa tatcttgcaa
     6721 tatcccgacg agcgtctgca cacggtggca aagcctgtcg aacaagtcga cgagcgcatc
     6781 cggaagctga ttgccgatat gtttgaaacg atgtacgaat cgcgcggcat cgggctggcg
     6841 gcgacgcagg tcgatgtgca cgagcgcgtg gtcgtgatgg atttgaccga agaccgcagc
     6901 gaaccgcgcg tgttcatcaa ccccgtcatc gttgaaaaag acggcgaaac cacttacgaa
     6961 gagggctgcc tgtccgtgcc gggcatttac gacaccgtaa cccgcgccga acgcgtcaag
     7021 gtcgaggctt tgaacgaaaa aggcgaaaag ttcacgctgg aggcggacgg cttgctggcg
     7081 atttgcgtgc agcacgagtt ggaccacctg atgggcatcg tgtttgtcga acgcctgtcg
     7141 cagctcaagc agggacgcat caaaaccaag ctgaaaaaac gtcagaaaca tacgatttga
     7201 cccttttgcc gtgccgtctg aacgctgcaa agttttcaga cggcacggtc ttgtccgaca
     7261 accttacgca tacgcaggaa tatgctatga aagtcatctt tgccggcacg cccgattttg
     7321 ccgccgccgc cttaaaagcc gttgccgccg ccggttttga aatcccgctg gtgctgaccc
     7381 agcccgaccg tccgaaaggg cgcggtatgc aactgaccgc cccgcccgtc aaacaagccg
     7441 cgctggaact cggtttgcgc gtggcacagc ccgaaaaact gcgtaacaac gccgaagccc
     7501 tgcaaatgct caaagaggtc gaggcagacg taatggtggt tgccgcctac ggtttgattc
     7561 tgccgcagga agtgttggat acgccgaaac acggctgcct caacatccac gcttcgctgt
     7621 taccccgttg gcgtggcgcg gcgccgattc aacgcgcgat tgaagccggc gatgccgaga
     7681 caggcgtgtg tattatgcag atggacatcg gtttggacac cggcgatgtg gtcagcgaac
     7741 accgctacgc catccaaccg accgataccg ccaacgaagt ccacgacgcg ctgatggaaa
     7801 tcggtgcggc ggcggttgtt gccgatttgc aacagcttca aagcaaaggc cgtctgaacg
     7861 cggtcaaaca gcccgaagaa ggtgttactt acgcccaaaa actaagcaaa gaagaggcgc
     7921 gtatcgattg gagcgaaagc gcggcggtta tcgaacgcaa aatccgcgcc ttcaaccccg
     7981 tgcctgccgc gtgggttgag tatcagggca agccgatgaa aatccggcgg gcggaagtgg
     8041 tggcgcaaca aggcgcggca ggcgaagtgt tgtcctgttc ggcggacggt ttggtcgttg
     8101 cctgcggcga aaacgcgctg aagattaccg aattgcagcc tgccggcggc aggcgtatga
     8161 atatcgcggc gtttgcagca ggacggcata tcgaagcagg gacgaagctg taaatccctt
     8221 cagacggcat tccgatccgc aaacgggaat gccgcctgaa accatcagtt gaagaaagcg
     8281 aatcacataa tatgagtatg gcacttgccc aaaaacttgc cgccgacagc attgcggcgg
     8341 ttgccgaagg acgcaacctt caggacgtgt tggcgcaaat ccgcaccgcg catcccgacc
     8401 ttacggcaca ggaaaacggc gcgttgcagg acatcgccta cggctgccag cgttatttgg
     8461 gcagtttgaa acatatgctc gcgcagatgc tgaaaaagcc gattgacaac ccgcagctcg
     8521 aaagcctgct tttggcggcg ttgtaccaac tgcattacac gcgcaacgcg ccgcacgccg
     8581 tggtcaacga agcggtggaa agcatagcca aaatcggacg cgggcagtac cgttcgtttg
     8641 ccaacgcgat tttgcgccgc tttttgcgcg aacgcgacaa gcttgcggct tcctgtaaaa
     8701 aagacgatgt ggcgaaacac aacctgccgc tgtggtgggt ggcttacctg aaaaaccatt
     8761 atccgaaaca ctggcacaac atcaccgccg cgctacaatc ccatccgccg atgacgttgc
     8821 gcgtcaaccg ccgacacggc aatgccgaaa gctatttgga aaaactggct gcggaaggta
     8881 ttccggccag ggcgttggac gaatatgcgg ttacgttgga agaagccgtg ccggtaaacc
     8941 gtctgcccgg tttttcagac ggcattgttt cggtacagga cttcggtgcg cagcaggcgg
     9001 cgtatcttct taacccgaaa gacggcgaac ggattttgga cgcgtgcgcc gcgccgggcg
     9061 gcaagacggg gcatatcttg gaactggcgg attgccgcgt taccgccttg gacatcgatg
     9121 caggccgtct gaagcgtgtg gaggacaata tcgcccgttt gggctttcag acggcatcgg
     9181 cggcgtgtgc cgatgcgcgg gatttggcgg cgtggtatga tggaaagcct ttcgacgcaa
     9241 tccttgccga cgtgccatgc accgcttccg gcgtggcgcg gcgcaatccc gacgtgaaat
     9301 ggctgcgccg tccgaccgac gcgctcaaaa ccgcccgcca gcaggaagcc ctgctggacg
     9361 cactttggca gacgctgaaa cagggcggca gaatgctgct tgccacctgt tccgtgttcg
     9421 tcgaagaaaa cgacgggcaa ttgcaaaaat tcctcaaccg ccatgccgat gccgaaccga
     9481 tcgaatcgcg ggtgctttta ccgaacaaac accaagatgg cttttattac gcgcttattc
     9541 aaaagcatta aacaatggct tgtgctgctg ccgatgcttt ccgttttgcc ggacgcggcg
     9601 gcggagggga tagatgtgag ccgcgccgaa gcgaggataa ccgacggcgg gcagctttcc
     9661 atcagcagcc gcttccaaac cgagctgccc gaccagctcc aacaggcgtt gcgccggggc
     9721 gtgccgctca actttacctt aagctggcag ctttccgccc cgataatcgc ttcttatcgg
     9781 tttaaattgg ggcaactgat tggcgatgac gacaatattg actacaaact gagtttccat
     9841 ccgctgacca accgctaccg cgttaccgtc ggcgcgtttt cgacagacta cgacaccttg
     9901 gatgcggcat tgcgcgcgac cggcgcggtt gccaactgga aagtcctgaa caaaggcgcg
     9961 ctgtccggtg cggaagcagg ggaaaccaag gcggaaatcc gcctgacgct gtccacttca
    10021 aaactgccca agccatttca aatcaatgca ttgacttctc aaaactggca tttggattcg
    10081 ggttggaaac ctctaaacat catcgggaac aaataatgcg ccgttttcta ccgatcgcag
    10141 ccatatgcgc cgtcgtcctg ttgtacggac tgacggcggc aaccggcagc accagttcgc
    10201 tggcggatta tttctggtgg atagttgcgt tcagcgcaat gctgctgctg gtgttgtccg
    10261 ccgttttggc acgttatgtc atattgctgt tgaaagacag gcgcgacggc gtattcggtt
    10321 cgcagattgc caaacgcctt tctgggatgt ttacgctggt tgccgtactg cccggcgtgt
    10381 ttctgttcgg cgtttccgca cagttcatca acggtacgat taattcgtgg ttcggcaacg
    10441 atacccacga ggcgcttgaa cgcagcctca atttgagcaa gtccgcattg aatctggcgg
    10501 cagacaacgc ccttggcaac gccatccccg tgcagataga cctcatcagc accgcctccc
    10561 tatccggcaa tatgggcagg gtgctggaac attatgccaa cgggtttaca cagttgtccc
    10621 tttacaatgc cgcaagcggc aaaatcgaaa aaagcatcaa cccgcacaag ctcgatcagc
    10681 cgtttccgga taaggcgcgt tgggaaaaaa tccaacaggc gggttcggtc agggatttgg
    10741 aaagcatagg cggcgtattg tacgcgcagg gctggctgtc ggcgggtacg cacaacgggc
    10801 gcgattacgc cttgtttttc cgtcagccgg ttcccaaagg cgtggcagag gatgccgtct
    10861 taatcgaaaa ggcaagggcg aaatatgctg agttgagtta cagcaaaaaa ggtttgcaga
    10921 cctttttcct ggcaaccctg ctgattgcct cgctgctgtc gatttttctt gcactggtca
    10981 tggcactgta tttcgcccgc cgtttcgtcg aacccgtcct atcgcttgcc gagggggcaa
    11041 aggcggtggc gcaaggcgat ttcagccaga cgcgccccgt gttgcgcaac gacgagttcg
    11101 gacgcttgac caagttgttc aaccacatga ccgagcagct ttccatcgcc aaagaagcag
    11161 acgagcgcaa ccgccagcgc gaggaagccg ccagacatta tcttgaatgc gtgttggagg
    11221 ggctgaccac gggcgtggtg gtgtttgacg aacaaggctg tctgaaaacc ttcaacaaag
    11281 cggcggaaca gattttgggg atgccgctta cccccctgtg gggcagcagc cggcacggtt
    11341 ggcacggcgt ttcggcgcag cagtccctgc ttgccgaagt gtttgccgcc atcggcgcgg
    11401 cggcaggtac ggacaaaccg gtccatgtga aatatgccgc gccggacgat gccaaaatcc
    11461 tgctgggcaa ggcaaccgtc ctgcccgaag acaacggcaa cggcgtggta atggtgattg
    11521 acgacatcac cgttttgata cacgcgcaaa aagaagccgc gtggggcgaa gtggccaaac
    11581 ggctggcaca cgaaatccgc aatccgctca cgcccatcca gctttccgcc gaacggctgg
    11641 cgtggaaatt gggcgggaag ctggatgagc aggatgcgca aatcctgacg cgttcgaccg
    11701 acaccatcgt caaacaggtg gcggcattga aggaaatggt cgaagcattc cgcaattatg
    11761 cgcgttcccc ttcgctcaaa ttggaaaatc aggatttgaa cgccttaatc ggcgatgtgt
    11821 tggcattgta tgaagccggt ccgtgccggt ttgcggcgga gcttgccggc gaaccgctga
    11881 tggtggcggc ggatacgacc gccatgcggc aggtgctgca caatattttc aaaaatgccg
    11941 ccgaagcggc ggaagaagcc gatgtacccg aagtcagggt aaaatcggaa gcggggcagg
    12001 acggtcggat tgtcctgacg gtttgcgaca acggcaaagg gttcggcaag gaaatgctgc
    12061 acaacgcctt cgagccgtat gtaacggaca aaccggcggg aacgggattg ggtctgcctg
    12121 tggtgaaaaa aatcattgaa gaacacggcg gccgcatcag cctgagcaat caggatgcgg
    12181 gtggcgcgtg tgtcagaatc atcttgccaa aaacggtaaa aacttatgcg tagcagcgat
    12241 attttaattg tagacgacga aatcggcatc cgcgacctgc tgtcggaaat cctgcaggac
    12301 gaaggttatt cggtcgcatt ggcggaaaac gccgaagagg cgcgcaagct gcgccatcag
    12361 gtacgcccgg cgatggtgtt gctggatatt tggatgcccg attgcgacgg catcaccctt
    12421 ttgaaggagt gggcgaaaaa cgggcagctc aatatgccgg tggtgatgat gagcgggcat
    12481 gccagcatcg ataccgccgt agaagcgacc aaaatcggcg cgctcgactt tttggaaaaa
    12541 ccgatttccc tgcaaaagct gctgtctgcc gtcgaaaacg cgttgaaaca cggtgcggcg
    12601 aaaatcgaaa cggggcctgt attcgacaag ctgggcaaca gtgcggcgat tcaggagatg
    12661 aactgcgaag tcgaagcggc ggcaaagcgc acgtcccctg tatttttgac gggcgaagcg
    12721 ggttcgccgt ttgaaaccgt ggcgcgctat ttccataaaa acggtacgcc gtgggtcagt
    12781 ccggcaaggg tcgaatatct gatcgatatg ccgatggaac tgttgcagaa ggcggagggc
    12841 ggcgttttgt atgtcggcga catcgcccaa tacagccgca atatccaaac cggcattgcc
    12901 tttatcatcg gcaaggcaga acgctgccgc gtcagggtgg tcgcatcggg cagcagggcg
    12961 gcaggttcag acggcattgc ctgcgaggaa aagctggcgg aactgctgtc ggaatcggtc
    13021 gtccgtattc cgccgctgcg tatgcagcat gaagatattc ccttcctgat acaggggatt
    13081 gcctgcaatg tggcggaaag ccaaaagatt gcgcctgcct cattcagtga agaggcactt
    13141 gccgcattga cccgttacga ctggccggga aatttcgacc aactgcaaag cgtcgttgca
    13201 acgctgttgt tggaggcgga cggacaggaa atcggcgcgg cggcggtttc ttcccttttg
    13261 gggcagaatg tgcctgccga gggagcggaa gatatggtgg gcgggtttaa tttcaacctg
    13321 cccctgcgcg aattgaggga ggaggtggag cggcgttatt tcgagtacca catcgcccaa
    13381 gaaggtcaga atatgagcaa agtggcgcag aaggtcggtt tggaacgcac gcacctttac
    13441 cgcaaactca aacagctcgg catcggcgtt tcgcgccggg cgggggaaaa aaccgaagaa
    13501 taggcccgga cggccggttt tccggctgcg ggcttttgtt ttcagacggc atttggtgca
    13561 aatgccgtct gaaatcgtaa ggggacggat tttatgacag aggacgaacg tttcgcgtgg
    13621 ctgcaattgg cgtttacgcc ctatatcggc gcggaaagct tcctgctgct gctgcgccgt
    13681 ttcggcagcg cgtcaaacgc cctgtccgca ccggcggaac aggtcgccct ggcggtgcgg
    13741 cacaagcagg cggccgaggc ttggcgcaat gcggaaaaac gcgcctcggc gcggcaggcg
    13801 gcagaagcgg cgttggaatg ggaaatgcgg gacggatgcc gtctgatgct gcttcaggat
    13861 gaagatttcc ccgaaatgct gacgcagggg ctgaccgcgc cgccggtttt gtttttgcgc
    13921 ggcaacgtgc ggctgctgca caagccttcc gccgccatcg tcggcagccg ccatgccacg
    13981 ccgcaggcga tgcggattgc caaagatttc ggcagggcgt tgggtgggaa aggcattccc
    14041 gtcgtgtcgg gtatggcttc gggcatcgac accgccgccc atcagggcgc gttgcaggca
    14101 gaaggcggca ccatcgccgt gtgggggacg ggcatagacc gcatttatcc gccgtccaat
    14161 aaaaaccttg cttatgaaat tgccgaaagg ggattgattg tcagcgagtt ccctttggac
    14221 acgcgtccgt ttgcaggcaa ctttccgcgc cgcaaccgcc tgattgccgc actgtcgcaa
    14281 gtaacgctgg tggtcgaggc cgccttggaa tccggttcgc tgattaccgc cggtttggcg
    14341 gcagaaatgg ggcgcgaggt aatggcggta cccggttcga tagacaaccc gcacagcaaa
    14401 ggctgccaca aactgattaa agacggcgcg aaactgacgg aatgcctgga cgacatcctg
    14461 aacgaatgcc cgcggctctt gcaaaatgag ggtgcttcgt catattctat aaataaggga
    14521 atgcctgaaa agcgcatcac tgccgttcag acggcatccg accagctgtc tctgcctgaa
    14581 ggcaaaatgc cgtctgaaaa gacggagaac cgacccgtcg gcggcagtat cttggacagg
    14641 atgggtttcg acccagttca tcccgacgtg cttgccggac agttggctat gcctgccgca
    14701 gatttgtatg ccgcactgtt ggaattggaa ttggacggca gcgttgccgc aatgcccggc
    14761 ggcagatacc agcgtatccg aacttgaacg cactttatat taaggaacac gaatgaccga
    14821 agtcatcgcc tacctcatcg aacatttcca agatttcgat acctgtccgc cgcccgaaga
    14881 cttgggtatg ctgcttgaag aagcgggttt cgatacgatg gaaatcggca acaccctgat
    14941 gatgatggaa gtattgctca acagctccga attttccgcc gaacccgccg acagcggcgc
    15001 attgcgcgtg tacagcaaag aagaaaccga caacctgccg caggaagtga tggggctgat
    15061 gcagtatctg attgaagaaa aagccgtcag ctgcgaacag cgggaaatca tcatccacgc
    15121 gctcatgcac attccgggcg acgaaattac cgtagatacc gccaaagtgc tgaccctgct
    15181 gcttttatgg gcaaacaaga gcgagctgcc cgtgttggtc ggcgacgagc tgatgagcgc
    15241 gcttttactc gacaacaaac ccacgatgaa ctgaagcggc ttcagacggc ccgcccgagg
    15301 ccgtctgaaa cgccggcatc aaaaccacca tccagagaac gacaaatggc gaaaaaccta
    15361 ttaatcgtcg aatccccgtc caaagccaaa accctgaaaa aatatttggg cggcgatttt
    15421 gaaatccttg catcctacgg acacgtccgc gacctcgtcc ccaaaagcgg cgcggtcgat
    15481 cccgacaacg gctttgcgat gaaataccaa ctcatcagcc gcaacggcaa acacgtcgat
    15541 gccatcgtcg ccggtgccaa agaagctgaa aacatctacc tcgccaccga cccggatagg
    15601 gaaggcgaag ccatttcctg gcatcttttg gaaatcctca aatccaaacg cggcttgaaa
    15661 aacatcaagc cgcagcgtgt cgtgttccac gaaatcacca aaaacgccgt gctcgatgcc
    15721 gttgcccatc cgcgcgaaat cgaaatggac ttggtcgatg cgcaacaagc ccgccgtgct
    15781 ttggactatt tggtcggctt taacctttcg ccattgttat ggaaaaaaat ccgccgaggt
    15841 ttgagcgcgg ggcgtgtgca aagccctgct ttgcgcctga tttgtgagcg cgaaaacgag
    15901 atccgcgcgt ttgaagcgca ggaatattgg acggtacatc ttgacagtca caaaggacgc
    15961 agcaagttca ccgccaaact cgcccaatac aacggcgcga aactcgaaca attcgacctg
    16021 ccgaacgaag ccgcgcaagc cgacgtgttg aaagaactcg aaggcaaaga ggccgtcgtt
    16081 accgccattg aaaagaaaaa gcgcagccgc aatcctgccg cgccgttcac cacttctacc
    16141 atgcagcagg atgccgtgcg caaactcggc ttcaccaccg accgcaccat gcgtaccgcc
    16201 caacagcttt atgaaggtat agacgtaggg cagggcgcga tcggtctgat tacctatatg
    16261 cgtactgaca gcgtgaattt ggcggatgaa gcgttaaccg aaatccgcca ttacatcgaa
    16321 aacaaaatcg gcaaagaata tctgccgagt gccgccaaac aatacaaaac caaatccaaa
    16381 aacgcccaag aagcgcacga agccatccgt ccgacttccg tgtaccgcac gcccgaaagc
    16441 gtcaaaccct tcctgagcgc agaccagttc aaactctatc aaatgatttg gcagcgtacc
    16501 gtcgcctgtc agatgacgcc tgccaaattc gaccaaacca ccgtcgatat taccgtcggc
    16561 aaaggcgtat tccgcgtaac cggacaagtg caaaccttcg caggcttctt gagcgtttat
    16621 gaagaaagca gcgacgatga agaaggcgaa gacagcaaaa aactgcccga aatgagcgaa
    16681 ggcgataaat tgcccgtgga caaactctac ggcgaacaac actttaccac tccgccgcca
    16741 cgctacaacg aagccacgct ggttaaagcc ctcgaagaat acggcatcgg ccgcccctcg
    16801 acctacgcca gcatcatctc cacgctcaaa gaccgcgaat acgttaccct tgagcaaaaa
    16861 cgcttcatgc ccaccgacac aggcgacatc gtcaacaaat tcctgaccga acacttcgcc
    16921 caatacgtcg attaccactt taccgccaaa ctcgaagacc agcttgacga aatcgccggc
    16981 ggcaaacgcc aatggattcc cttgatggac aaattctgga aaccgttcat caaacaagtg
    17041 gaagaaaaag aaggcatcga acgcgccaaa tttaccacgc aggaacttga tgaaacctgc
    17101 ccgaaatgcg gcgaacacaa actgcaaatc aagttcggca aaatgggccg tttcgttgcg
    17161 tgtgccggct atcccgagtg cagctacacg cgcaatgtca atgaaaccgc cgaagaagcc
    17221 gcagagcgca tcgccaaagc cgaagccgaa caggcagaac tcgacggacg cgaatgtcct
    17281 aaatgtggtg gacggctggt gtacaaatac agccgtaccg gcagtaaatt catcggttgc
    17341 gccaactacc ccaaatgcaa acacgtcgag ccgctggaaa aaccgaaaga taccggcgtc
    17401 cagtgtccgc aatgtaaaaa aggcaacctc gtcgagcgta aatcccgcta cggcaaactg
    17461 ttttacagtt gcagcaccta tcccgactgt aactacgcca catggaaccc acccgttgca
    17521 gaaagctgtc caaaatgcgc atggccggtt ttgaccatca aaaccaccaa acgctggggt
    17581 gtagaaaaag tctgcccaca aaaagaatgc ggctggaaag aacagattga accgcccgcg
    17641 ccgaaggagt aagattaggt tggtttgaaa gagaaaaggt cgtctgaaaa attttcagac
    17701 gacctttgct tttctgtgat tggtttattt gaatccgcgt gttgttttaa agtccgataa
    17761 aatccggttc atttcaggcg caaacaaggc gatgtaatcg taagatagac cgcgactggc
    17821 actgggatgg ggaaagcaga cgacttcgca atcttcaaac gattggaatt tgacattgaa
    17881 acgtgtaccg tcaaattctt tttgcaccgt ctccagcggt ttggtctgct taccgaccaa
    17941 ctgctcgaag cgtggcagta cattttggtt gttcagaaaa tccgccaacc tgctgcccat
    18001 gaagaggatg actttcggac gcagtttttc gatgtggtag agaaaattat cgatgtgctc
    18061 gggttgtgtg aacttgtctg gattgtcgat agtgttgccc tgtgtagcag cccagttggt
    18121 ttgaaccagg gatttttcaa atgcaccgcc caatccattt tcgtctaagg ggtgtcccca
    18181 catttcaaac caatttttta tcgtattatc gtaacgccac ttttttgcct gctctccgaa
    18241 atagagggat ttgtttgcaa atgtatggtc gattttgttt tcagggagtt tgtattcacc
    18301 tgctacataa gcagcctcat cggctttact ccaaccccat tcatagccac aaatcattaa
    18361 gccatgtttg tcgttgtagc ctttgaacag gctgttgctc aaattcaaat ccttcatcat
    18421 gaactcttcc ttttaaaatt taagagcgat tgacttcaat gtttttagat ggggtggaaa
    18481 aatccttgtg taggcaacat aaattcaata aatttcttga taattcgaaa cctactaata
    18541 gcgcacctat aaaagctttt tcattacgtt cagcatgacg gtcacgtcgt tcatattttt
    18601 tacgcttgct gttccctgtt attacagcta agccaagtga tatggcgaga attgcccaaa
    18661 caatagtact taaataacaa ttttccccat actatcaata aggaaagaaa aaacctttta
    18721 gtattaggtc gatagattat aatccatgcc catgaaaatg caataagagt tatacataag
    18781 atacaagctg acaggatttc tttattttta attaaataac ttagagcgat gaggatgaca
    18841 ggtgtcaaga aaataatagt tacatcccga tagctataaa agaaaactgc cctattttga
    18901 atgtggagat gtgcacagaa tccaatatag ctaaggataa tagttaatat aataaaaaaa
    18961 gaccaccaag ggtgaagaga taggaattcc atgttttccc tttttttgta aaaaggaaaa
    19021 aatcttatct aatagttaat tgcctaacca gccagaagaa gtttaaaatc tatcccaata
    19081 attcaaccat ctatacagaa agttcagctt atggaaaccc acgaaaaaat ccgcctgatg
    19141 cgcgaattga ataaatggtc ccaggaggat atggcggaaa agctggcgat gtcggcagga
    19201 gggtatgcca aaatcgaacg gggcgaaacg cagttgaatg tcccgcgttt ggagcagttg
    19261 gctcagattt tcaaaatcga tatgtgggac ttgctcaaat cgggcggtgg tgggatggtg
    19321 tttcagatta atgaaggtga tagtggtggc gatattgcgt tgtatgcgtc gggtgatgtt
    19381 tcgatgaaaa tagaattttt aaaaatggag ttgaaacact gcaaagaaat gttggaacaa
    19441 aaagacaaag aaatcgagct gctccgcaag ctgaccgaaa ccgtttaaac agatatgccg
    19501 tctgaaaaaa gttttcagac ggcatattct ttgacaggtc ttgtataata ccgtttgaac
    19561 ttacaggttt ttgattatgg cggcaggcaa acataccaaa cacagcaacc gggtacgcat
    19621 tatcggcggg caatgccggg gcaggaaatt gagtttcaca tccgccgacg gactgcgtcc
    19681 gacacccgac agcgtgcgtg aaaagctgtt taactggctg ggacaggatt tgacgggtaa
    19741 aacggttttg gatctcttcg gaggcagcgg cgcactcggt atagaagccg cttcgcgcaa
    19801 cgccaaacgc gtgctgattt cggataacaa ccgccaaacc gtgcagacct tgcagaaaaa
    19861 cagtcgcgaa ctgggtttgg ggcaggtgca aatcgtctgt tcagacggca tcgcatattt
    19921 gaagaccgta tccgaacagt ttgatgttgt ctttctcgac ccgccgtttg catggcagga
    19981 ctggcaaatc ctgtttgatg ccttgaagcc gtgcctgaac ccccgagcat tcgtctatct
    20041 cgaggcgggt acgctgccgg atattcccga ttggctgacg gaatatagag aagggaaatc
    20101 ggggcagagt acattcgaat taagggtttt ccaagtggct gaataatatg cgctttgata
    20161 atcatttccg agttgtaaac attcgtttgc aaccgtccgg ttcaaaaaaa ccttgtgcta
    20221 taatccgcgc ccgcccggtt ttgataattt agtggaaaag gaaaagaaat gtcgcttttt
    20281 attaccgacg agtgcatcaa ctgcgacgta tgcgaacccg aatgccccaa tgatgccatt
    20341 tcccaaggcg aggaaattta cgaaatcaac cccaacctct gcacgcagtg cgtcggacac
    20401 tacgatgagc cgcagtgcca gcaggtttgc ccggtggact gcatcctgat tgacgaagaa
    20461 catcccgaaa cccatgacga gttgatggcg aaatacgaaa agattatcca gtttaaataa
    20521 attcttttta aaacatcaaa ttatgtctgt tttgaaataa aatcaaaaaa aaacttgacg
    20581 gaaaagcaag ccgctaataa actaacgttc tcttttggag ggattcccga gcggtcaaag
    20641 ggggcagact gtaaatctgt tgcgaaagct tcgaaggttc gaatccttct ccctccacca
    20701 aaattcttac ttggggcagt agcgagtaat gcgggtgtag ctcaatggta gagcagaagc
    20761 cttccaagct tacggtgagg gttcgattcc cttcacccgc tccaaacaat taggcccatg
    20821 tagctcaggg gtagagcact cccttggtaa gggagaggtc ggcagttcaa atctgcccat
    20881 gggcaccatc tctcgattat tcatttcttt aaggcttaga tatataggat attg
//
LOCUS       DAWFAD010000019        20678 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWFAD010000019 DAWFAD010000000
VERSION     DAWFAD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20678
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.019"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(63..3668)
                     /gene="putA"
                     /locus_tag="WGO49_000526"
     CDS             complement(63..3668)
                     /gene="putA"
                     /locus_tag="WGO49_000526"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ0305676.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDKQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHCRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDCLIKSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDTKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRNDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     SVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADELDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRAGEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWIHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
     gene            3660..3827
                     /locus_tag="WGO49_000527"
     CDS             3660..3827
                     /locus_tag="WGO49_000527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305677.1"
                     /translation="MKHFLDSPKFFGGQAASVRPEYGNIVINPQMKYKASNAGRVGAS
                     VSLKMKRVKHK"
     gene            complement(3917..5443)
                     /gene="putP"
                     /locus_tag="WGO49_000528"
     CDS             complement(3917..5443)
                     /gene="putP"
                     /locus_tag="WGO49_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ0305678.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            5926..6246
                     /locus_tag="WGO49_000529"
     CDS             5926..6246
                     /locus_tag="WGO49_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ0305679.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            6260..7756
                     /locus_tag="WGO49_000530"
     CDS             6260..7756
                     /locus_tag="WGO49_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ0305680.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECQVPQMPFEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQACIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            7862..8707
                     /gene="ftsN"
                     /locus_tag="WGO49_000531"
     CDS             7862..8707
                     /gene="ftsN"
                     /locus_tag="WGO49_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0305681.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAATEAEQSDAEKAADKADEVEEK
                     AGEPEREKSDGQAVRKKVLTEEREQTVREKAQKKDAETVKKQAVKPSKETEKKAPKEE
                     KKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTSDKAEATHYLQMGAYADRR
                     SAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHEVASLIR
                     SIESK"
     gene            8710..9354
                     /locus_tag="WGO49_000532"
     CDS             8710..9354
                     /locus_tag="WGO49_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0305682.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEGEDYLVLDKPIPQKQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVKLSGL
                     KYQANPAVFKAVYEQKIRLENRSVAEKWALSQKGFDGKKLMRAYDSPAAAAAASKMQQ
                     LTEQYRIDSTPTVVVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            9387..10208
                     /locus_tag="WGO49_000533"
     CDS             9387..10208
                     /locus_tag="WGO49_000533"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ0305683.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIDFHSNHK
                     VFEITIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIVDVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(10490..10565)
                     /locus_tag="WGO49_000534"
     tRNA            complement(10490..10565)
                     /locus_tag="WGO49_000534"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10530..10532),aa:Ala,seq:ggc)
     gene            complement(10573..10649)
                     /locus_tag="WGO49_000535"
     tRNA            complement(10573..10649)
                     /locus_tag="WGO49_000535"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10613..10615),aa:Met,seq:cat)
     gene            complement(10781..11998)
                     /locus_tag="WGO49_000536"
     CDS             complement(10781..11998)
                     /locus_tag="WGO49_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0305684.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLI
                     AEEIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLTGYL
                     PQSHFLLNLGITELLAQVGKTDSPAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVD
                     WAGFRFGDICHKL"
     gene            12277..12732
                     /gene="mraZ"
                     /locus_tag="WGO49_000537"
     CDS             12277..12732
                     /gene="mraZ"
                     /locus_tag="WGO49_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ0305685.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            12729..13715
                     /gene="rsmH"
                     /locus_tag="WGO49_000538"
     CDS             12729..13715
                     /gene="rsmH"
                     /locus_tag="WGO49_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ0305686.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQAALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PELPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGPIPEQSQRKTSEWQ"
     gene            13712..13975
                     /gene="ftsL"
                     /locus_tag="WGO49_000539"
     CDS             13712..13975
                     /gene="ftsL"
                     /locus_tag="WGO49_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ0305687.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVEHQR"
     gene            14036..15781
                     /locus_tag="WGO49_000540"
     CDS             14036..15781
                     /locus_tag="WGO49_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ0305688.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            15806..17284
                     /locus_tag="WGO49_000541"
     CDS             15806..17284
                     /locus_tag="WGO49_000541"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ0305689.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDGYGAELIGRLKKDCPDLAVYSYGFSEHADIRITDF
                     TASSDGIEAVFRTPWGEGKCRTRLLGRFNAQNLAACIALLCANDYPLDKVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAVRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            17307..18953
                     /locus_tag="WGO49_000542"
     CDS             17307..18953
                     /locus_tag="WGO49_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ0305690.1"
                     /translation="MKQSARIKNMNQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWRYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            19079..20437
                     /locus_tag="WGO49_000543"
     CDS             19079..20437
                     /locus_tag="WGO49_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ0305691.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDRVAMDGALKVDDTLAALQTLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNALRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGVDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALALAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            20440..20616
                     /locus_tag="WGO49_000544"
     CDS             20440..20616
                     /locus_tag="WGO49_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305692.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
BASE COUNT         4792 a         5059 c         5996 g         4831 t
ORIGIN      
        1 gccgtctgaa aggttttcag acggcattgg ttcacggccc gcgcccgggg atttcggtaa
       61 aatcagtcgg cgaccgccat caggctggcg ttgccgccgg cggctgtggt gttgacgctg
      121 caagagattt cttcaaacac ttgcaggatg tcgagtccgt tttccgaagg gaggatgcgg
      181 atgagtgcgc cgtcgtgggc ggcaagttcc tgtttgcgcg cgctgtccaa aggcgacagg
      241 gcggcaacgt ggctgatgtc ggcggtttcg ggtttgccgt tgaccagcag cagaccttcc
      301 aagtcggcgg tgtaggaagc cagcgggctg tcgggttcga ccactgcctg tacgccggag
      361 gcggcaagtt cggttagggc gcagaaggct tgcaggaggc tgccgccgtg tatccaaacg
      421 cgtttgggcg cgtgccatga gatgctgttg cgctcgccgg tcggtccggt aaggacggtt
      481 tcggcacggc gcagggtgcg gatgcgggcg tgtcccaaag cggccgctgc ggcttttttc
      541 tcttcggcgt tgaacggtag tttgtgaacc agtgcttcga ggcgtttgag tgcggcttcg
      601 tccgcctgtc cgatttggct cagggtcggg gcaacccatt cgccggcgcg ggtcagtttt
      661 tgcaggtaga atgaaccgcc tgctttggga cctgtgccgg acagaccgtg tccgccgaag
      721 ggctgtacgc cgacgactgc gccgacgatg ttgcggttga cgtaaacgtt gccggcttcg
      781 atgcggctgc ggatgtggcg taccgtgcct tcgatgcggc tgtgtacgcc gtgggtcagg
      841 gcgtagcctt tgctgttgat ttggtcgatg acgctgtcga gttcgtcggc gcggtagcgg
      901 acgacgtgca ggacgggacc gaagacttcg cgttgcagtt cgttgaggtt gttcaattca
      961 aacaggatgg ggcggacgaa cgtggatttt tcggaatcga catcggcagc ggttttgact
     1021 tcgtggtaag acttggcaac gcctttcatt ttgttgatgt ggttcaacag gttttgctgc
     1081 gcttcggcat cgatgacgga gccgacatcg gtggtgagtt gaatcggttt gccgacgacg
     1141 agttcgtcca tcgcgccttt gatcatgtcg agcatacggt cggcaacgtc ttcttggacg
     1201 cacaagatac gcagggcgga gcagcgttgt cccgcgctgt cgaaggcgga gttcaatacg
     1261 tcggcgcaga cttgttcggc aagtgcggtg gaatcgacaa tcatggcgtt ttgtccgccg
     1321 gtttcggcaa tcaggacggg attgtcgttg cgtttggcaa gggctttgtt gatcaggcgc
     1381 gccacttcgg tcgagccggt gaaaattacg ccgccgatgc gggcatcgtt ggtcaatgcc
     1441 gcacccacgt cgcctgcgcc gaggacgagt tgcagggcgg aagtcgggat gccggcttcg
     1501 tgcatgaggg aaacggcata accggcaatc aggctggttt gttcggcggg tttggcgata
     1561 acggtgttgc ctgccgccaa tgcggacacg acttcgccgg taaagatggc gagcgggaag
     1621 ttccacgggc tgatggcgac aatcgcgccg acggcttttg tgtcttgagg cagggtgtgt
     1681 tcggcttcgt ttgcgtagta gcggcagaaa tcgacggctt cgcgcacttc ggcaatggcg
     1741 ttgttcagcg ttttgcccgc ttcgcgcacg gcaagcatca ttagcgcggg ggtgtgctgt
     1801 tcgagcaggt cggcgaaacg gcgcaggcag gcggcgcgtt cggcggcagg tgtcgcactc
     1861 cattcgggga acgcggcaac ggctgcgcca accgcttctt gggcaagcgc ggcatcggca
     1921 aagctgactg tgccgacgat gtcgtcgtgg tcggcaggat ttttaatcgg ttgcgcttcg
     1981 ccgacatcgc gggctttgcc gttgacgatg gatgcggcgt ggaagtcttg tgcggcggct
     2041 ttgttcatct gttcttgaag ctgctgcaat acgttttcgt tgctcaagtc cacgccttgc
     2101 gagttcagac ggcatttgcc gtacaaatcg cgcggcagcg gcagggcgtt gtgcaggtgg
     2161 atgccttgtt cggcgatggt gtcgaacggg ctcttaatca ggcagtcgat gctgatgttt
     2221 tcatcgacga tttggttgac gaaagacgag ttcgcgccgt tttccaacag gcggcgcacc
     2281 aagtaggcga gcagggtttc gtgtgtgccg accggggcgt acacgcgcac gcggcggcct
     2341 aagttttgcg ggccgacgac ttggtcgtac agggtttcgc ccatgccgtg caggcattgg
     2401 tgttcaaagt ctttgccttt gcccatttgg tagattgcgc ctaaggtgta ggcgttgtgc
     2461 gtggcaaatt gcgggaatac cgcgtcttgc gcggaaagca gtttgcgcgc gcaggcgagg
     2521 taggagatgt cggtgtggac tttgcgggtg taggtcggat agccgttcaa gccgtccact
     2581 tgcgcccatt tgatttcgct gtcccaatac gcgcctttga cgaggcggat catcagtttt
     2641 tggttgttgc ggcgggctag gtcaatcagg tagtcgataa cgaacggaca acgtttttgg
     2701 taggcttgga caacgaaacc gatacctttg tagccagcca agtcagggtc tgaaaccaaa
     2761 gcctccatca aatccaaaga cagctccaag cggttggctt cttcggcatc gatgttgata
     2821 ccgatatcgt attttttgcc caaaaggaac agctctttca ggcgcggcaa cagttcgccc
     2881 atcacgcggc agtgttgggc gcgcgagtag cgcggatgga tggcggagag tttgacggaa
     2941 atgccgttgc cttcgtaaac gccttgtcct gccgcatctt tgccgatggc gtggatggct
     3001 tcgacatagt cgcggtagta gcggtcggca tcggcttggg tgtaggcggc ttcgcccaac
     3061 atatcgaagg agaagcggta gcccattttt tcgcgttctt tgccgttttg cagggcttct
     3121 tcaatggtct gtccggtgac gaactgtttg cccaacaggc gcatggcgta attcacgcct
     3181 tggcggatga gcggtgcgcc gcctttgctg atcaggcggc tgagtgcgga actcatttgt
     3241 ttgtcgtttg tggcggtcag tttgccggta atcagcaggc cccaggcggc agcattgacg
     3301 aagagggaag ggctgttgtt caaatggctt ttccagttgc cgtctgaaat cttgtcggca
     3361 atcaggcggt cgcgcgtggc gttgtcgggg atacgcagca gggcttccgc cagacacatc
     3421 agtgcgatgc cttcttcgct ggagagtgag aattcgtgca tcagcgcatc cacgccgccg
     3481 gctttggtgc ggccggcgcg gacttgggta accaaacggc gggcaagctc ggaggcggcg
     3541 ttgcgctctt cgtcgctcat ctgtgcacgt tgcaacatat cctgtacggc ttcgatttca
     3601 ttacggcggt aggcatcggt tatcgcttgg cgcagggcag tttgtgccgg aaatgcaaaa
     3661 tgaaacattt tttggattct ccaaagtttt tcggggggca ggcggcatcg gtgcggcctg
     3721 aatacggtaa tatcgtaata aatccgcaga tgaaatacaa ggcttcaaat gcgggcaggg
     3781 taggtgcttc cgtttctttg aaaatgaaac gggtaaaaca caaataaggc ctgtatgcag
     3841 gcaaggttta tttgtgtttg acccggaaac gggttcagac ggcacgaacc gggatgccgt
     3901 gccgtctgaa aggggtttat cgggcggcgc ggtaatctgc gtcggctttt tcaaagcgtt
     3961 cttgggtttc gcgcgaaggt tctttgttga acagggaaac caatacggcg acgattaagc
     4021 aaacaataaa gcccgggacg atttcgtaca tcgtcaacaa gccgctttcg cctgccgctt
     4081 gagccggttt ttttacccat tccgcccata cgactacggt taaggcacct gcaaccatac
     4141 ccgacaacgc gccgtaggca gtgatgcgtt tccacaatac ggacagaatc acaatcgggc
     4201 cgaacgccgc accgaaacct gcccacgcat aagacaccag tcccaatact ttgctgttcg
     4261 gatcggaagc aatcaggatg gaaatcacgg caatcgccaa gaccatcagg cggccgaccc
     4321 ataccagttc cgactgttgc gcgtttttac gcaaaaagcc tttgtagaag tcttcggtaa
     4381 tcgcgctgga gcaaaccaaa agctggcagg acagggtgga catcaccgcc gccaaaatcg
     4441 cgctcaaaat gatgccggcg atccaagggt tgaacagcag ggtggaaagc gcgatgaaga
     4501 tgcgttcgtg gttgccgctc atagaagaaa ctttgtcggg atttgcaccg aaatacgcaa
     4561 tgccgaaata accgaccgcc accgcgcccg caaggcacaa cgccatccaa gtcatgccga
     4621 tgcggcgtgc ggataccagc gatttcgcgc tttcggccgc cataaagcgc gccaaaatgt
     4681 gcggctgtcc gaaatagccc aagccccatg cggcggtgga aatgatgccg atgacggtcg
     4741 taccggcaaa caggctgccg tattctttgc ccgtgcctgc ggcgacgctt tgaatcgcgg
     4801 cagacatctg ttccgcgccg cccaagccca gatagaccat caccggcgtt aaaatcaggg
     4861 cgaaaatcat caaagaagcc tgcagcgtat ccgtccagct gaccgccaag aagccgccca
     4921 agaaggtata ggcgatggtc gcgcccgcgc ccagccacat tgcctgattg taagtcatac
     4981 cttcaaacag gctttggaac agggttgcgc ccgccacaat gcccgaggcg caataaatcg
     5041 tgaagaagaa caggataatc agtgcggaaa ccactttcat caagtgtccg cccgcgccga
     5101 agcggtggaa gaaataatcc ggcagcgtca gcgcattgtt ggcgtattcg gtatgcacgc
     5161 gcaggcggcc cgccaccaag agccagttga aatacgcgcc gaccaagagg ccgatggcaa
     5221 tccaagcctc attcaaaccg ctcaggtaaa tcgcgcccgg cagacccatc agcagccagc
     5281 cggacatatc ggacgcgcct gccgacatcg cggtaacaaa cgggcctagg ctgcgcccgc
     5341 ccaaaatata atcgtcgaaa ttgcgcgtag aaaaataggc ggcaagcccg atgagaagga
     5401 ctgcaaccag atagattgca aaagtaatgt acatgggatt catgtgctat tcctcgtcta
     5461 aaacttcaga attacaggct ttgaaattgc aagcaacttg cgcctgaaat gtttttctaa
     5521 taaaagtaca acggaaaatc cggatacccg aaagggggat tcggataaat tatcttcaat
     5581 cacaataaga tatgtaataa aaccatatga aattgtaaat aatccgtttc agcataaccc
     5641 aatttctgtt gtttgcaaag cacttaatgg cttaaaaagc cgagtttgaa acgatgcgcg
     5701 tcggaaaaat catttaaaac agcatattgt tttgtagtgt cttgtaatca ggcgttgcgc
     5761 ggaatatgaa atccgttttc aggcggcagg tgttttgagg tgtaatttag caaccgcaaa
     5821 ggaggcgcgg tatgttttgc cgattatccg ccgcccgttt tcagacggca tttttcctta
     5881 tacaatagcc gattgaattt gatatgttca ggaaggatac agattatgtt cggcaagcag
     5941 ctttttgagg aagtcggctc gaaaatcagc gaaaccatcg ccaacagccc tgccaaagat
     6001 gtggaaaaaa atattaaggc gatgctgggc ggcgcgttca accgtatgga tctggttacg
     6061 cgcgaagaat tcgacatcca gcagcaggtt ttaatcaaaa cccgtaccaa actggcggct
     6121 ttggaagcgc gtttggcaaa actcgaagcc gcgcaaaatc ccgaacgggc agcattggaa
     6181 gcggctgaag ccgctgccga agaagccgtc gccgaaatca ggcagcaaac cgaagccggc
     6241 gaataaggcc gtctgaaata tgtcgcttgc cttggtttac agccgcgcct tgagcggtat
     6301 gaatgcgccg ttggtcgaag tggaagccca ccttgccaac ggtctgccgc atttcaacat
     6361 cgtcgggctg cccgatacgg aagtcaaaga aagtcgcgac cgcgtccgtg ccgccattat
     6421 tcaaagcggt tttgaattcc ccgccaaaaa aatcaccgtc aacctcgccc ccgccgacct
     6481 gcccaaagag tcggggcgtt tcgatttgcc gattgcaatc ggcatccttg ccgcatcggg
     6541 gcaggttgcg cccgaaaaac tggcggaata cgagtttgcg ggggaattgg cactgtcggg
     6601 gctgttgcgc cccgtgcgcg gcgcgttggc gatggcgtgg cagggtatgc aggcaaaacg
     6661 tgcatttgtt ttgcctgaag aaaatgcagg acaagccgcc gtgatgcgcg gcattaccgt
     6721 ttacggcgcg cgctctttgg gcgaagtcgc cgcccatttg aacggcatcg aacctttggc
     6781 gcaaaccgaa tgccaagttc ctcagatgcc gtttgaacat ggcggacaac ctgatttgtg
     6841 cgatgtgaaa ggtcagcaca ccgcgcgcct tgctttggaa atcgctgccg caggcggaca
     6901 cagcctcttg atgatgggtc cgccgggaac gggcaagtct atgctctccc aacggctgcc
     6961 cggcatcctg ccgccgctga ccgaagacga attggtagaa gtttgggcat tgcgttcgct
     7021 cctgcccaac caccagcaac aactcgacag caaccgtcct ttccgcagtc cgcatcacag
     7081 cgccagcgcg gcggctatgg tcggcggcgg ttccgacccg cgtccgggcg agatttcatt
     7141 ggcgcaccac ggcgtattgt ttttggacga gctgcccgag ttcgaccgca aagttttgga
     7201 agttttgcgc gaaccgttgg aaaacggcga aatccacatt tcccgcgcgg cgcgccaagc
     7261 cgtctatcct gccaaattcc aacttgttgc cgccatgaac ccctgcccgt gcggttatct
     7321 cgggcatccc gtcaaaccct gccgctgcac gcccgaaagc gtcgcgcgtt accgcagcaa
     7381 gatttccggg ccgctgctcg accgcatcga tttgaccatc gaagtcccca gcctgtccgc
     7441 cgccgaactg atgcagcagg aagcgggcga aagcagcgcg tccgttttgg aacgcgttat
     7501 cgccgcccgc gacaaacaat acgcacggca gggcaaagtc aacgccgctt tgagcgtcag
     7561 cgaactcgat tcccaagcct gcatccaaaa agaagcgcag gaagcattgg gcggcctgct
     7621 ggaaaaactc tccctttccg cccgcagctt ccaccgcatc atgcgcgtgg cgcgtacatt
     7681 ggcggatttg gctggcgacg aagaagtcgg cagaagccac gtcatgaaag ccataggttt
     7741 ccgtcgtgct ttataggaat gggaatggaa gcaggttttg cccaaatatg gcgatattgt
     7801 tagaatatcc gcccgtaagc aaacggcgtt aatgccgtct gaaacacatt aaggtatgtt
     7861 tatgaacaaa ttttcccaat ccggaaaagg tctgtccggt tttttcttcg gtttgatact
     7921 ggcgacggtc attattgccg gtattttgtt ttatctgaac cagagcggtc aaaatgcgtt
     7981 caaaatcccg gcttcgtcga agcagcctgc agaaacggaa atcctgaaac cgaaaaacca
     8041 gcctaaggaa gacatccaac ctgaaccggc cgatcaaaac gccttgtccg aaccggatgc
     8101 tgcgacagag gcagagcagt cggatgcgga aaaagctgcc gataaagccg acgaggttga
     8161 agaaaaggcg ggcgagccgg agcgggaaaa gtcggacgga caggcagtgc gtaagaaagt
     8221 gctgacggaa gagcgtgaac aaaccgtcag agaaaaagcg cagaagaaag atgccgaaac
     8281 ggttaaaaaa caagcggtaa aaccgtctaa agaaacagag aaaaaagctc caaaagaaga
     8341 gaaaaaggcg gaaaaggaaa aagttgcacc caaaccgacc ccggaacaaa tcctcaacag
     8401 cggcagcatc gaaaaagcgc gcagtgccgc cgccaaagaa gtgcagaaaa tgaaaacgtc
     8461 cgacaaggcg gaagcaacgc attatctgca aatgggcgcg tatgccgacc gccggagcgc
     8521 ggaagggcag cgtgccaaac tggcaatctt gggcatatct tccaaggtgg tcggctatca
     8581 ggcgggacat aaaacgcttt accgggtgca aagcggcaat atgtctgccg atgcggtgaa
     8641 aaaaatgcag gacgagttga aaaaacatga agtcgccagc ctgatccgtt ctatcgaaag
     8701 taaataatta tgaagctcaa acatctgttg ccgctgctgc tgtcggcagt gttgtccgcg
     8761 caggcatatg ccctgacgga aggggaagac tatcttgtgt tggataaacc cattcctcaa
     8821 aaacagtcgg gcaaaattga ggttttggaa tttttcggct atttctgcgt acattgccat
     8881 catttcgatc ctttgttatt gaaattgggc aaggcattgc cgtctgatgc ctatttaagg
     8941 acggagcacg tggtctggca gcctgaaatg ctcggtctgg caagaatggc tgctgcggtc
     9001 aagctgtcag gtttgaaata tcaggcaaac cctgccgtgt ttaaagcagt ttacgaacaa
     9061 aaaatccgct tggaaaacag gtcggttgcc gaaaaatggg ctttgtctca aaaaggcttt
     9121 gacggcaaaa aactgatgcg cgcctacgac tctcctgcgg cagcggctgc tgcatcaaaa
     9181 atgcagcaat tgacggaaca gtaccgcatc gacagtacgc cgaccgttgt cgtcggcgga
     9241 aaataccgcg ttatcttcaa taatggcttt gacggcggtg ttcatacgat taaagaattg
     9301 gttgccaaag tcagggaaga acgcaagcgt cagacccctg ctgtacagaa atagccgaac
     9361 tcccgtatcc gaaagaagcg caagcaatgg attttctgat tgtcctgaaa gccctgatga
     9421 tgggcttggt agaaggtttt accgaatttt taccgatttc cagcaccgga catttgattg
     9481 tgttcggcaa tctgattgat tttcacagca atcacaaggt ttttgaaatt accatccagc
     9541 tcggtgcggt tttggcggta gtgtttgaat accggcagcg tttcagcaat gtgttgcatg
     9601 gcgtgggaaa agaccggaaa gccaaccgtt tcgtccttaa tcttgccatt gcttttatac
     9661 ctgccgccgt gatggggctg ttgttcggca aacaaatcaa agagtatctg tttaacccct
     9721 tgagtgttgc agtcatgctg gttttgggcg gtttttttat tttgtgggtg gagaaacgcc
     9781 aaagccgggc agagcctaaa attgtcgatg ttgatgcatt gcgtccgatt gatgcgttga
     9841 tgatcggcgt tgcccaagtg tttgcactgg ttccgggtac gtcccgttcg ggcagtacga
     9901 ttatgggcgg gatgctttgg ggaatcgagc ggaaaacggc aacggagttt tcatttttct
     9961 tggccgttcc gatgatggtt gcagcaacgg cttatgatgt cctgaagcat taccggtttt
    10021 tcaccctgca tgatgtcggt ttgattctga ttggctttgt tgccgctttt gtttccggtt
    10081 tggtggcggt aaaagcgttg ctgaggtttg tttccaagaa aaattatatt ccttttgcct
    10141 attaccgcat tgtttttggc attgccatca ttatattgtg gctgtcaggc tggataagtt
    10201 gggaatgaaa ccataaaccc gacctgaaga cattattcgg gtcgggtttg tttggcgggc
    10261 tgatatagtg aattaacaaa aatcaggaca aggcgacgaa gccgcagata gtacggcaag
    10321 gcgagccaac gctgtaccgg tttaaattta attcactata aaatcaggac aggcggggcg
    10381 ataggtttaa agtcgattgc ctgttttgaa ggcagtggtt tattctttat ttgctggcaa
    10441 tcaggcaata aaaaagcaca taccttttta cggtctgtgc ttttttatct ggtggaggta
    10501 agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc gctctaccaa ctgagctata
    10561 cccccgaaaa tttggtggcg aatcagggac tcgaaccccg gacacaagga ttatgattcc
    10621 tctgctctaa ccgactgagc taattcgccg tttcgtgaag acgctattat atgtttttct
    10681 gtttttttga caagccgtat tttttaattt tgaattagtt gactgttttt aaatgttaaa
    10741 aagtttatgt cgtctgaagc ggattcaggc ggcatgaggg ttagagtttg tggcagatgt
    10801 cgccgaagcg gaatcctgcc cagtcgacgc cgatattttt accgaatgcg atgactttaa
    10861 acaattctcc catttcatgc tggtcaatca gtttctgaac ggcagcagct tcacggatgt
    10921 aggcgggcga gtctgttttt cccacttgtg ccaacagttc ggttatgccc agattcagca
    10981 gaaaatggga ctggggcaga tagcctgtca aatccagtcc tgcatccgta ccggcttggg
    11041 caatgtcggt aaagttgaca tgcgcggtca ggtcggccaa cccgatgaag tcaaaaggat
    11101 tgtggataac gtgatgtcgg tagtgtccga tcagagtacc ttgattgcgt tgagggtggt
    11161 aatactgcgc tgcatcaaaa ccgtagtcga tgaatatcat acccccgcgt tcaagccttg
    11221 aggcaagggt gcggataaag gcatattgtt gcggatgtag ttcgctggtg tagggataat
    11281 ctgtttgagg aaaatagagg gaagccaagg cagatagctg taagtcgtgc agcggtcgtg
    11341 ccgaataggt aaaacggtca ttatctaggc aaacgccgac atgctcgaat gagccgcctt
    11401 catttttacg gacgatttcg acaggcatgg catcgagtac ttcgttgccg atgatgatgc
    11461 cgtcaaacgc ttcgggaagt gcggtcaagt ggacaacttt ttgagatgct tccggtgcgc
    11521 gtgcttgaat caggtttttc tgacgtgctg ccagctccgg cgatatttca ataatatagt
    11581 aacggctgat gccgtccgaa atgctgccca acaaatcggc ggcaagctgt ccggttcccg
    11641 cgccgaattc atagatattg cccgccgttt gggatagaag ttcttgaagt tggcgtgcca
    11701 gtgtctgtgc aaacagagag gtgagggtcg gtgcggtaat aaaatccccg gtattgccga
    11761 ttttatggct gccgccggtg tagtagccgt attgcggagc gtataaaacc aattccataa
    11821 aacgtgaaaa tggaatccag ttgccgtgtt tgccgatttc ttcggcaatg agggtttgca
    11881 gtttgagcga gaattgccgt gcttcgggag aggggagggg gaggggcatg ataagtgtta
    11941 gcttgtgtaa atttattgga tttcccgaca tattacacgt tggtacgggt gctgtcatgg
    12001 ctttatctta atactatata ttgtgtttat attattaaat taatcatata tagttgttta
    12061 ttggttcgat tattctgtac cgcacccgcc gtgccgttgt cgtcattttt tatcttattg
    12121 tttttaaaag gaataaaaat ttcagatatg ttaatgagtt ttcatgccct gatttgaccg
    12181 agtgtttaaa atttcttata gtgtcgattg gtggggaatt gtggggcaaa gtgtctcttt
    12241 tacccttgtg attttgattt cggcttggga catgtcatgt tcggcggcgc acacgaatta
    12301 agcatcgaca gtaaggggcg gttggctgtt cctgccaaat tccgtgacat tctgtcgcgc
    12361 ctctatacgc ctgccgtagt ggtaacgctc gagtcgaaac acaagctgtt gatgtaccct
    12421 gttgcggagt gggaaaaggt tgcggcgcaa cttttaaact taaaagtggc ggataaccct
    12481 gttttgcggc ggtttcaaaa tcttttgctg cataacgcgg aaattttgga atgggacagc
    12541 gccggccggg tgctgcttcc tgccggactg aggaaaaggg tggatttcga ccgtgaagtc
    12601 gttttggtcg gtcgtgccaa ccgtttggag ctttggggtc gcgagcagtg ggaggctgag
    12661 atggttcagg ctttggatga cgatcctgac gaacttgcct tccagttggg tcagacggat
    12721 ttgcaattgt gagtggagca gaaagttacc ggcatatcac ggtcttgctg aatgaggcgg
    12781 tggatgcgct tgccgtgcgc gaagacggtg tctatgtgga cggtacgttc ggcaggggag
    12841 ggcattcccg gctgattttg tcgcgtttgg gcgatgcggg gcggttgatt gttttcgaca
    12901 aagaccctca ggcgattgct gtggcagaag agctggcgcg ttcggacaaa cgggtcggtg
    12961 tcgtgcatgg cggttttgct tcgtttcagg cggcattgga cggtttgggt atcggcaagg
    13021 tggacggtgc gctgtttgat ttggggattt cgtctccgca aatcgatgac ggcagccgcg
    13081 gtttcagctt ccgtttcgat gcccctttgg atatgcgtat ggatacgacg cgcggtatgt
    13141 ctgccgcaga gtggatagcg gttgcgtcgg aacaggattt gcacgaggta atcaagaatt
    13201 atggtgaaga gcggtttagc cgccagattg cgcgcgccat tgttgcgcaa cgggcggaaa
    13261 gtccaatcga tacaacccgc aagctggcgc agatcgtggc acaaaacgtc cgtactcgcg
    13321 agcgggggca ggatcctgcg acgcgcacct tccaggcggt ccgcatcttt attaaccgcg
    13381 agcttgaaga agtaggggca gtattgccgc aggtcatgtg tcgtctgaaa gagggcggac
    13441 gtttggcggt cattgctttc cattcgttgg aagaccgcat tgtgaagcag tttgtcaaaa
    13501 aatattcgca acacgcgccc ctgccgcgct gggcggcggt cagggaagcg gatttgcccg
    13561 agctgcccct gaaaatcgtg ggcagggcat taaagccggg tgaggcggaa attgccgcca
    13621 atccgagggc gagaagtgcg gttttgcgtg tggcggagcg gactgccggt ccgataccgg
    13681 aacaatcaca gagaaaaacg tctgaatggc aatgaacaaa ttgaatttcc ttctgctgct
    13741 tgcggtgtgc gtttccgctt tttccgttgt gatgcagcaa aaccagtaca ggctcaactt
    13801 cacagctttg gataaggcga aaaaacagga aatcgccttg gagcaggatt atgcgcaaat
    13861 gaggctgcaa caggcgcgtt tggcgaacca cgaagcgatc agggcggcgg cagaaaaaca
    13921 aaacctccat ccgccggttt cgggcaatac ctttatggtg gagcatcaaa gatagaagca
    13981 gcctgtgtgc cggaatcgga ttcctgcgtc aggataataa taacgagaag taaaaatgtt
    14041 gattaagagc gaatataagc ctcggatgct gcccaaagaa gagcaggtca aaaagccgat
    14101 gaccagtaac ggacggatca gcttcgtcct gatggcaata gcggtcttgt ttgccggtct
    14161 gattgcccgc ggactgtatc tgcagacggt aacgtataac tttttgaaag aacagggcga
    14221 caaccggatt gtgcggactc aaacattgcc ggctacacgc ggtacggttt cggaccggaa
    14281 cggtgcggtt ttggcgttga gtgcgccgac ggagtccctg tttgccgtgc ctaaagagat
    14341 gaaggaaatg ccgtctgccg cacaattgga acgcctgtcc gagcttgtcg atgtgccggt
    14401 tgatgttttg aggaacaagc tcgaacagaa aggcaagtcg tttatctgga ttaagcggca
    14461 gctcgatccc aaggttgccg aagaggtcaa agccttgggt ttggaaaact ttgtatttga
    14521 aaaagaatta aaacgccatt acccgatggg caacctgttt gcacacgtca tcggatttac
    14581 cgatattgac ggcaaaggtc aggaaggttt ggaactttcg cttgaagaca gcctgcatgg
    14641 cgaagacggc gcggaagtcg ttttgcggga ccggcagggc aatattgtgg acagcttgga
    14701 ctccccgcgc aataaagccc cgaaaaacgg caaagacatc atcctttccc tcgatcagag
    14761 gattcagacc ttggcctatg aagagttgaa caaggcggtc gaataccatc aggcaaaagc
    14821 cggaacggtg gtggttttgg atgcccgcac gggggaaatc ctcgccttgg ctaacacgcc
    14881 cgcctacgat cccaacaggc ccggccgggc agacagcgaa cagcggcgca accgtgccgt
    14941 aaccgatatg atcgaacccg gttcggcaat caaaccgttt gtgattgcga aagcattgga
    15001 tgcgggcaaa accgatttga acgaacggct gaatacgcag ccttataaaa tcggaccgtc
    15061 tcccgtgcgc gatacccatg tttacccctc tttggatgtg cgcggcatca tgcagaaatc
    15121 gtccaacgtc ggcacaagca aactgtctgc gcgtttcggc gccgaagaaa tgtatgactt
    15181 ctatcatgag ttgggcatcg gtgtgcgtat gcactcgggc tttccgggcg aaactgcagg
    15241 tttgttgaga aattggcgca ggtggcggcc tatcgaacag gcgacgatgt ctttcggtta
    15301 cggcctgcaa ttgagcctgc tgcaattggc gcgcgcctat accgcactga cgcacgacgg
    15361 cgttttgctg ccggtcagct ttgaaaaaca ggcggttgcg ccgcaaggca aacgcatatt
    15421 caaagaatcg accgcgcgcg aggtacgcaa tctgatggtt tccgtgaccg agccgggcgg
    15481 caccggtacg gcgggtgcgg tggacggttt cgatgtcggc gcgaaaaccg gcacggcgcg
    15541 caagttcgtc aacgggcgtt atgccgacaa caaacacatc gctaccttta tcggttttgc
    15601 ccccgccaaa aacccccgtg tgattgtggc ggtaaccatt gacgaaccga ctgcccacgg
    15661 ttattacggc ggcgtagtgg cagggccgcc cttcaaaaaa attatgggcg gcagcctgaa
    15721 catcttgggc atttccccga ccaagccact gaccgccgca gccgtcaaaa caccgtctta
    15781 atccgagtat caacgagatt gttttatgtt cagcaagtta acccctttgg ctgaaaccgg
    15841 catcccgacc ctgtcgtgtg caaacgcagc agggcgtttg ttgcattcag acagccgcca
    15901 aatcaaacaa ggcgatattt tcgttgcctg tccgggcgaa tatgccgacg gccgcagtta
    15961 tatccccgcc gccgttgcca acggcgcggc ttttgttttt tgggacgacg acggcaaatt
    16021 cgcgtggaat cccgaatgga aagtccccaa tcaaggcatc aaagatttga aacaccgtgc
    16081 cggcatattg gcggcgcaag tttacggcaa cgtttcagac ggtctcaaat tctggggcgt
    16141 aaccggaacc aacggcaaaa cctccatcac gcaatggctg gcgcaagctg ccgatttgtt
    16201 gggcgaaaaa accgccattg tcggcacggt cggcaacggc ttttggggtg cattggaaga
    16261 aaccacgcat accacacccg cccccgtcga tgtccaaacc ctgctctacc gtttccgtca
    16321 acaaggcgca acagccgccg cgatggaagt ctccagccac ggtcttgacc agtcgcgcgt
    16381 caacggcgtg ccattccgca gcgcaatctt taccaatctc acccgagacc acctcgacta
    16441 ccacggcacg atggaagcct atggcgccat caagtcgcgc ctgttttact ggcatggctt
    16501 gaaacacgcc gttatcaatg tagatgacgg atacggcgca gaactcatag gccgtctgaa
    16561 aaaagactgc cccgatttgg ccgtttatag ctacggcttc agcgaacacg ccgacatccg
    16621 cattaccgac tttaccgcct cttcagacgg catagaagcc gtattccgaa ccccgtgggg
    16681 tgaagggaaa tgccgcacac gcctgctcgg acggttcaac gcgcaaaacc tcgccgcctg
    16741 catcgccttg ctgtgcgcca acgactatcc gttggataag gtattggatg tgctggcaaa
    16801 aatccgtccc gcttcagggc gcatggactg catcatgaac agcggcaagc ccttggtcgt
    16861 cgtcgattac gcccacacgc ccgacgcatt ggaaaaagca ctcgccacct tgcaggaaat
    16921 caaaccgcag ggtgcggctt tatggtgcgt attcggttgc ggcggcaacc gcgatcgcgg
    16981 caaacgcccg ctgatgggcg cggcagccgt acagggcgcg gataaagtcg tcgtcaccag
    17041 cgacaacccg cgtttggaaa atccgcacga catcatcaac gacatcctgc ctgccgttcc
    17101 cgcgcccgaa tgcgtcgaag ccgaccgtgc cgccgccgtc cgttatgcgg ttgaacaagc
    17161 cgccgcaaac gacatcatcc tgattgccgg caaagggcat gaaaactatc aggatgtaca
    17221 aggcgtgaag caccgttttt ccgatcttga aatcgtcgga caggctttgt taactcgtaa
    17281 ataatgggat attcggacgg catcgtatga aacaatccgc ccgaataaaa aatatgaatc
    17341 agacattaaa aaatacattg ggcatttgcg cgcttttagc cttttgtttt ggcgcggcca
    17401 tcgcatcagg ttatcacttg gaatatgaat acggctaccg ttattctgcc gtgggtgctt
    17461 tggcttcggt tgtattttta ttattattgg cacgcggttt cccgcgcgtt tcttcagttg
    17521 ttttactgat ttacgtcggc acaaccgccc tatatttgcc ggtcggctgg ctgtatggtg
    17581 cgccgtctta tcagatagtc ggttcgatat tggaaagcaa tcctgccgag gcgcgtgaat
    17641 ttgtcggcaa tcttcccggg tcgctttatt ttgtgcaggc attatttttc atttttggct
    17701 tgacagtttg gagatattgt gtatcggggg gggtatttgc tgacgtaaaa aactataaac
    17761 gccgcagcaa aatatggctg actatattat tgactttgat tttgtcctgc gcggtgatgg
    17821 ataaaatcgc cagcgataaa gatttgcgag aacctgatgc cggcctgttg ttgaatattt
    17881 tcgacctgta ttacgatttg gcttccgcgc cggcacaata tgccgccaag cgtgcccaca
    17941 ttttggaagc agcaaaaaaa gcgtcaacat ggcatatccg tcatgttgcg cccaagtata
    18001 aaaattatgt tgtggttatc ggtgagagcg cgcgttcgga ttatatgaat gtttacggtt
    18061 tcccattgcc cgatacgcct tttttgagtc agaccaaagg gctgttgata aacggttacc
    18121 aatcgaccgc ccacgcgacg aatctttcgc tgccgcagac tttggggctg ccgggagaac
    18181 cgaacaataa catcgtcagc ttggcgaagc aggcgggttt tcggacggcg tggctgtcta
    18241 atcaaggaat gttggggcat tttgccaacg aaatttccac ctatgcccta cgcagcgatt
    18301 atccgtggtt tacccaaagg ggtgattatg gcaaaagcgc ggggttgagc gaccgccttt
    18361 tgttgccggc gttcaaacgg gttttgatag gaaatgcagg cacgaagcct cggctgattg
    18421 tgatgcacct gatgggttcg cacagtgatt tttgcacacg tttggataag gatgcgcggc
    18481 ggtttcagta tcaaactgaa aaaatatcct gctatgtttc caccatcgcg caaaccgata
    18541 aatttttaga agatacagtt aagatattga atgaaaataa agaaagctgg tctttggttt
    18601 acttttccga ccacggtttg atgcatgtcg gtaaaggcgg cgagcgaacg ttgacacatg
    18661 gtgcgtggaa gcgtcaaagc tacggcgtgc cgctggttaa aatttcgtcc gatgacacgc
    18721 ggcgcgaaat gattaaagtg aggcgcagcg cgtttaattt tttacgcgga ttcggcagtt
    18781 ggacgggtat cgaaaccgac gagttgcccg atgacggcta tgatttttgg gggaatgttc
    18841 ccgatgtgca gggcgaaggc aataaccttg cctttatcga cggactgccc gacgaccccg
    18901 cgccgtggta tgcgggaaaa ggcaaatcga ctaaaaatac gtctaaaaaa tgatacgtac
    18961 agaaaaaatg ccgaatgaga atgggaaaat aatctgtgtt ttaccacagc aaaacaggcg
    19021 ataaaaaaaa tcagccgcta ccgatgtgtc cgccgcccga atattaacga aagtaaatat
    19081 gaaaccactg gacctaaatt tcatctgcca agccctcaag cttccgatgc cgtctgaaag
    19141 caaacccgtg tcgcgcatcg taaccgacag ccgcgacatc cgcgcgggcg atgtgttttt
    19201 cgcattggcg ggcgggcggt ttgatgcgca tgattttgtt gaagacgtat tggctgctgg
    19261 tgcggcggcg gttgtggttt cgcgcgaaga tcgcgttgca atggatggcg cgttgaaagt
    19321 cgatgacacg cttgccgcat tgcaaacgct ggcaaaggcg tggcgcgaga atgtgaaccc
    19381 gtttgtgttc ggtattaccg gctcgggcgg taagacgacg gtgaaggaaa tgttggctgc
    19441 ggtattgcgc cgccgtttcg gcgataatgc cgttttggcg acggcaggca acttcaacaa
    19501 ccacatcgga ttgccgttga ctttgttgaa attaaacgaa aaacaccgct atgccgtgat
    19561 tgaaatgggt atgaatcatt ttggcgaact ggcggttttg acgcaaatcg ccaaacctga
    19621 tgccgccttg gttaacaacg ccctgcgtgc ccatgtcggc tgcggtttcg acggagtggg
    19681 cgatattgcc aaagcgaaaa gcgagattta tcaaggctta tgttcagacg gcatggcact
    19741 gattcctcaa gaagatgcca atatggctgt cttcaaaacg gcaacgctta atttgaatac
    19801 gcgcactttc ggcgtcgata gcggcgatgt ccacgcggaa aatatcgtgc tgaaaccgtt
    19861 gtcgtgcgaa tttgatttgg tgtgcggcga tgagcgcgcc gccgtggtgc tgcctgttcc
    19921 cggccgccac aatgtccaca acgccgccgc tgccgccgcg ctggctttgg ctgcgggttt
    19981 gagtttgaac gatgtggcgg aaggtttgaa aggcttcagc aatatcaaag gccgtctgaa
    20041 cgtcaaatcc ggaatcaagg gcgcaaccct gattgacgat acttataatg cgaaccctga
    20101 cagcatgaaa gctgcgattg acgtgttggc gcgtatgcct gcgccgcgta ttttcgtgat
    20161 gggcgatatg ggcgaactgg gtgaggacga agccgccgcc atgcacgccg aagtcggcgc
    20221 gtatgctcgc gaccaaggca tcgaagcggc ttattttgtc ggcgacaaca gcgtcgaagc
    20281 ggcggaaaaa tttggcgcgg acggtttgtg gttcgccgcc aaagacccgt tgattcaagt
    20341 gttgcgccac gatttgcccg aacgcgccac cgtgttggtg aaaggttcgc gctttatgca
    20401 gatggaagaa gtggtcgagg cattggagga taagtgaaaa tgaaaagtcg acgttttttt
    20461 aaagccttat tgttgattgc cgcgctggtc ggcgcgtttt atgccggaat gcagacgcag
    20521 gcgtatcttt atgaagattt atgtttagac ttgggtggcg gcaaaaatcc ggggagttac
    20581 ccgatttgcg tgattgagaa agtccctgca cgttaatctg caaaagccgt ccgaaacctt
    20641 gccgggcggc aagccaacct caaacgggcg caggcccg
//
LOCUS       DAWFAD010000020        20410 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWFAD010000020 DAWFAD010000000
VERSION     DAWFAD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20410
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.020"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            135..971
                     /gene="cysT"
                     /locus_tag="WGO49_000545"
     CDS             135..971
                     /gene="cysT"
                     /locus_tag="WGO49_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ0305693.1"
                     /translation="MLALKTPDVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     SGFWNTITEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGSGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
     gene            1226..2086
                     /gene="cysW"
                     /locus_tag="WGO49_000546"
     CDS             1226..2086
                     /gene="cysW"
                     /locus_tag="WGO49_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ0305694.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            2083..3156
                     /locus_tag="WGO49_000547"
     CDS             2083..3156
                     /locus_tag="WGO49_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ0305695.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGNILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKEQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(3213..4739)
                     /gene="ilvA"
                     /locus_tag="WGO49_000548"
     CDS             complement(3213..4739)
                     /gene="ilvA"
                     /locus_tag="WGO49_000548"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ0305696.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSHGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGDETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            4887..6056
                     /locus_tag="WGO49_000549"
     CDS             4887..6056
                     /locus_tag="WGO49_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ0305697.1"
                     /translation="MTAHKILPVLLSIILGVSHATAASPAPNKPAVHAAPTFQTPETL
                     TAAHIVIDLQSKQILSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWARLTGQ"
     gene            complement(6181..6753)
                     /locus_tag="WGO49_000550"
     CDS             complement(6181..6753)
                     /locus_tag="WGO49_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ0305698.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQPVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(6820..7803)
                     /locus_tag="WGO49_000551"
     CDS             complement(6820..7803)
                     /locus_tag="WGO49_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ0305699.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(7970..8045)
                     /locus_tag="WGO49_000552"
     tRNA            complement(7970..8045)
                     /locus_tag="WGO49_000552"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8010..8012),aa:Gln,seq:ttg)
     gene            complement(8054..8129)
                     /locus_tag="WGO49_000553"
     tRNA            complement(8054..8129)
                     /locus_tag="WGO49_000553"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8094..8096),aa:Gln,seq:ttg)
     gene            complement(8142..8217)
                     /locus_tag="WGO49_000554"
     tRNA            complement(8142..8217)
                     /locus_tag="WGO49_000554"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8182..8184),aa:Gln,seq:ttg)
     gene            complement(8225..9070)
                     /gene="ispE"
                     /locus_tag="WGO49_000555"
     CDS             complement(8225..9070)
                     /gene="ispE"
                     /locus_tag="WGO49_000555"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ0305700.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            complement(9080..9661)
                     /locus_tag="WGO49_000556"
     CDS             complement(9080..9661)
                     /locus_tag="WGO49_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ0305701.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHISSFAAEGRLAV
                     KAEGKGSYANFDWTYQPLVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGIPSETETPERCAARTR"
     gene            complement(9801..11639)
                     /locus_tag="WGO49_000557"
     CDS             complement(9801..11639)
                     /locus_tag="WGO49_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305702.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMFALSKLPDKREALRGLDKIIEKPPAGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            complement(11804..12640)
                     /locus_tag="WGO49_000558"
     CDS             complement(11804..12640)
                     /locus_tag="WGO49_000558"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ0305703.1"
                     /translation="MQIIHTIQELRAWRKNAGTVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLERQAADHLTAAGWVVDYVEIRRADTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            complement(12763..13554)
                     /gene="panB"
                     /locus_tag="WGO49_000559"
     CDS             complement(12763..13554)
                     /gene="panB"
                     /locus_tag="WGO49_000559"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ0305704.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTVKFVKNFMQGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            complement(13696..13771)
                     /locus_tag="WGO49_000560"
     tRNA            complement(13696..13771)
                     /locus_tag="WGO49_000560"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13736..13738),aa:Thr,seq:tgt)
     gene            complement(13806..13881)
                     /locus_tag="WGO49_000561"
     tRNA            complement(13806..13881)
                     /locus_tag="WGO49_000561"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13846..13848),aa:Thr,seq:tgt)
     gene            14047..14838
                     /locus_tag="WGO49_000562"
     CDS             14047..14838
                     /locus_tag="WGO49_000562"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ0305705.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            14893..15534
                     /locus_tag="WGO49_000563"
     CDS             14893..15534
                     /locus_tag="WGO49_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0305706.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(15612..16535)
                     /locus_tag="WGO49_000564"
     CDS             complement(15612..16535)
                     /locus_tag="WGO49_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0305707.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGATLDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            16699..17568
                     /locus_tag="WGO49_000565"
     CDS             16699..17568
                     /locus_tag="WGO49_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ0305708.1"
                     /translation="MKRIFLPALPAILPLSTYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENSRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLAGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDISAGISHTFLKDDKNPALIGFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPVVLSLTAAYRINGSKTLSSNTKYKAGNYWMLNPNISFAAN
                     DRISLTGGIQWLGKQPDRLDGKKESARNTSTYAHFGAGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            17611..18084
                     /locus_tag="WGO49_000566"
     CDS             17611..18084
                     /locus_tag="WGO49_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305709.1"
                     /translation="MKKQITSAVMMLSMIAPAMANGLDNQAFEKYVFHTQADAPMQLA
                     ELSQKEMKETEGAFLPIVAGALMGGAISAWANHGISKIKTGQFASTRSTLAATAGGMI
                     GGGYSSAMLRGAGITTSVFARSAWKGGNAIPNAVIRANGAALNQSSSAAMGKHRR"
     gene            18646..18918
                     /locus_tag="WGO49_000567"
     CDS             18646..18918
                     /locus_tag="WGO49_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305710.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIG
                     YVLIKNILVSVSDEDGTKYLAIYLSNLFFGTVLMYVLVKRLSKKPS"
     gene            19081..19626
                     /locus_tag="WGO49_000568"
     CDS             19081..19626
                     /locus_tag="WGO49_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305711.1"
                     /translation="MKKQITAAMMILTMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            19608..19973
                     /locus_tag="WGO49_000569"
     CDS             19608..19973
                     /locus_tag="WGO49_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305712.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            20031..>20410
                     /locus_tag="WGO49_000570"
     CDS             20031..>20410
                     /locus_tag="WGO49_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305713.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
BASE COUNT         5150 a         5505 c         4828 g         4927 t
ORIGIN      
        1 acatcgcgcc cgcttgagaa actgccaacc gctttataac aaattcgtct ttgcaccaaa
       61 ctttccattc tttccgtttt tcggacggca tcgttaaagt agtccttcct tttccttatt
      121 ttcagcattg ttttatgtta gcgctcaaaa cgcccgacgt actgcccggc ttcaaactca
      181 gccttggtct gaccgtattg tgcctgtcgc tgcttgtggt cttgccgttt gcgatgatgg
      241 cggcgaaggc ggcggaaatc ggctggagcg gattttggaa cacgattacc gaacccaatg
      301 tcttggcggc cgtctggctg agtttgcgga tgtcgtttta tgcgatgctg accaatgtcg
      361 tgttcggcac gctggtggcg tgggtattgg tgcgttatga attcccgggc aagggtctgg
      421 cgaacgcgct ggtcgatttg ccgtttgcgc tgccgacggc ggttacgggt atcgcgttgg
      481 cgaccctgta tgcgcccaac ggttggatag gccgtttttt cgagcctttg ggcatcaaaa
      541 tcgcgtttac gcccgtcggc atttggattg cgctggtcgt cgtcagcctg ccctttatcg
      601 tccgcgccgt gcagccggtg ttggaggaat tatcgggcga atatgaggaa gcggcggcga
      661 ctttgggcgc aaaccgcttt acgacgttcc gccgcgtgct gctgccggaa atcacgccgg
      721 cactcttgac cggctcggga atgatgttcg cgcgggcaac gggggaatac ggctcggtga
      781 tttttatcgc gggcaatatt ccgatggttt ctgaaatcct gccgctgatt attacgggca
      841 agctggaaca gttcgacgtg cagggcgcgt cggcggtggc gttgtttatg ctgctggttt
      901 cgtttgtgat tctgtttgcg ctgaacgtga tgcagtgggc gttgggcagg cgttcgggcg
      961 cgaagggttg aggtcgtctg aaatacctgt taccgtcatt cccgcgcagg cgggaatcca
     1021 ttggtgaatt tcggctgcct tatttatttt ctgttttcct gttgccctgc ggtggattcc
     1081 cgcctgcgcg ggaatgacgg tagctagacg tttttattcc cttaatcaat aaaaggttgt
     1141 ctgaaaacga atccgcccca caaaaaacgg tttttcagac ggcatccgaa caccttaaaa
     1201 caaccaaccg gggaacacca ccgccatgaa accctattcc gccaatccca acctgaccga
     1261 accgcgccgg ctgcgcgtgt tgctgattgc cgccgcgctg ggctttctgc tgctgatgct
     1321 ggtcgtgccg ctcgtcgccg tgttttacga agccttaaaa ggtggttggg atttgtacct
     1381 gaaatcctta aacgatcccg aagcgtggtc tgccatcaaa ttgacgctga ttaccgcgct
     1441 gattgtcgtt cccgtcaatg ctgtattggg tgtggcgatg gcgtggctgc tgacccgttt
     1501 tgattttcgc ggcaagcagt tgctgaccac cctgctcgat ttgccgtttt ccgtatcgcc
     1561 cgtggtggcc ggtttaatgt tcgtcttatt gttcggcgcg cacacggcat tgggtggctg
     1621 gctcgaagcg caaggcatac agattatctt cgccatcccc ggcattattc tggcgacgct
     1681 gttcgttact ttcccctttg tcgcacgcga aatcatcccg ctgatgcagg ctcagggcga
     1741 cagcgaagaa caggcggcat tgatactcgg cgcaagcggc tggcagatgt tttggcgcgt
     1801 taccctgccc aacatcaaat gggcgttact ctacggcatc atcctcacca acgcccgcgc
     1861 gatgggcgag ttcggcgcgg tcagcgtggt atcgggacac atacgcggcg aaaccaacac
     1921 cgtcccgctt ttggtcgaaa tcttctacaa cgaatacaac ttcaccggcg cattcgccct
     1981 ctccggcgta ttggcacttt tggcactggc gacgctggcg gtgcagaaca tcattaccaa
     2041 attacaagac aaaaaactcg ccgccgccga aaggaacgca gtatgagtat caccatccaa
     2101 aacttaaaca aacacttcgg caattttcac gcgctgaaaa acatcaacct caacgtcccc
     2161 accggcaaac tcgtttccct gctcggcccg tccggctgcg gcaaaaccac acttttacgc
     2221 attatcgccg gactggaaaa cgccgacggc ggcaatatcc tgtttgacgg gcaagacgta
     2281 accgccaaac atgtgcgcga gcgcaaagtc ggcttcgtgt tccaacacta cgcccttttc
     2341 cgccatatga acgtgtttga caacgtcgct ttcggtttga ccgtattgcc caagtccgaa
     2401 cgcccgtcca aagaacaaat ctgcactaaa gtcgaagaat tgctgaaact cgtacaactc
     2461 tcgcatttgg caaaatccta tccgcaccaa ctctccggcg ggcaacgcca gcgcatcgcc
     2521 ctcgcccgtg cgcttgcggt cgaaccgaaa ctcctgcttt tggacgaacc cttcggcgcg
     2581 ttggacgcca aagtacgcaa agaattacgc acctggctgc gcgatatcca tcacaacctg
     2641 ggtgtaacca gcattctggt tacgcacgac caagaagaag ccctcgaagt ttccgacgaa
     2701 atcgtcgtga tgaaccacgg caaaatcgaa caaaccggca gcgccgaagc tatttaccgc
     2761 aaacccgaaa atgccttcgt taccgagttc ctcggcgaaa ccgacgcttt tgaaggacgc
     2821 atcgaaaaag gcttctggca ttataacggc ttcgcgtgga aattggacgc acaatacaaa
     2881 tggcaggaac aaaccgccac cggctatatc cgcccgcacg aatggcagat cgccgccgaa
     2941 cacgaaacac cgatgatttg tgccgaaatc gaaaaagtcc acgccgtcgg ggcattgacg
     3001 catattttgg tgaaacacgg caaacaagac gtgcatatca cgctggctgg cagcgatgcc
     3061 gcgcgttacc caatcgccga aggcaaagaa ttgaagctga ttccgaaaca ggtttatgtc
     3121 ttctctcaaa acgaactgat tgaatattcg atttaaccat gaaagcgcaa tgccgtctga
     3181 aaggctttca gacggcattg tgctttcaag cgtcaggcaa gaaacagctt gtacgcggca
     3241 ttttgcgttt cctcgtgata gctgtatccc agactttcca agaaaccgtc aaatgcggcg
     3301 gcatcgtgcg gcggcacatc gataccgacc aaaatccgcc cgtaatccgc accgtggttg
     3361 cggtaatgga agagcgtaat gttccaaccg ccctgcatat ggttcaaaaa acgcgccaac
     3421 gcccccggac gctccggaaa ctcaaaactg accaaacgct cgttttctac tttgtccgtc
     3481 cgccctccaa ccatataacg gatatggatt ttggcaatct catcatcagt caggtcgaca
     3541 ttgggcaatc ccgcctcatc caaccggctg ccgataaccg ccaaatcctg cgggcctgcc
     3601 gcttgaagtc cgacaaagat atgcgctttt tcatcgtctc cgtagcggta gttgaactcg
     3661 gtaatattcc tatttcccaa tatattgaca aacttaagga agctgccgcg ttcttcgggg
     3721 atggtaacgg caaaaatacc ttcgttgccc tcgcccaatt cactacgctc cgacacatgg
     3781 cgcaaacggt gaaaattcat attcgcaccg ctggtaacgg caatcagggt ttggttttcc
     3841 gcgccttctc gggcgatata ggctttcaga cccgccaacg ccaacgcgcc cgccggctcg
     3901 gtaatgctgc gcgtgtcatc gaaaatatcc ttgaccgcgc cgcaaaccgc atcggtatcg
     3961 actgtaatga tttcatccaa aagttctttg cagaggcgga aggtttcgtc tccgacaact
     4021 ttgaccgcag tgccgtctga aaacagcccg acatctttca aatggacgat ttcacccgct
     4081 tcgaccgact gcttcataca gcaggaatcg ttggtctgaa cgccgataac tttgatttcg
     4141 gggcggacct gcttgataaa tgccgccacg cccgccgcca aaccgccacc gcctatcggt
     4201 acgaatacgg cgcggattgg atcgggatgc tggctgacaa tttccatccc caccgtcccc
     4261 tgtcccgcaa tcacatcagg atcatcaaac ggcgcgatat aggttaaccc ttctttttcc
     4321 gccaactcca tcgcataatc gtaggcatcg ttgtatgaaa cgccccgcaa aaccacctcg
     4381 ccgccatggc ttttaaccgc atccactttg attttcggcg tagtttccgg cataacgata
     4441 acggcacggc aacccaaacg ctgtgcggac aatgccacgc cttgagcatg attgcccgcg
     4501 cttgccgcaa tcacgccgca agcgagcgca tctttcggca acttggacat tttgttgtac
     4561 gcgccgcgta ttttgaacga aaaaaccggt tgcaaatctt cgcgtttcaa aaggatgttg
     4621 tttttcaaac gtacagaaag gctgcgtgcc ggttccaaag gcgtttcgac cgcgacatca
     4681 tagacagatg ccgtcaggat gcggatgagg taattggaat aaggaagggg cgtgttcata
     4741 attcaatatg ggataatcgg tttattaaaa tcgcaaaacc caaaaccata cgcccaagac
     4801 ggcgcgaaag caagaaaaat ccgcccgatc agacacccta agcgtataat cggcagactg
     4861 aaacacgcac acaattagaa tatttcatga cagcacataa aatcctgccc gtcctgcttt
     4921 ccatcatctt aggcgtttct cacgcaacgg ccgcatcgcc cgcgcccaac aaaccggcgg
     4981 tacacgccgc ccccacgttc caaacacccg aaaccctcac agcggcacac atcgttatcg
     5041 accttcaaag caaacagatt ttatccgcca aaaacatcaa tacccctgtc gaaccggcgg
     5101 cactaaccca actgatgacc gcatatctgg ttttcaaaaa catgaaatcg ggaaatatcc
     5161 gatctgaaga aaacttaaaa atacccgaat ccgcatgggc ttcagaagga agcagaatgt
     5221 ttgtacgtcc cggcgatacg gtcagcaccg acaaactctt aaaaggcatg attgcactat
     5281 ccgcaaacga tgccgcccta acccttgccg gccggctggg caacggctcg attgaaaatt
     5341 ttgtgcaaca aatgaacaaa gaagcccgac gcttgggcat gaagaacact gtattcaaaa
     5401 atccgacagg cttgagtaga gaaggacagg tttccaccgc caaagacctc gcccagctgt
     5461 ctgaagcatt gatgcgcgac tttccggaat attacccgct gttttccatc aaatctttca
     5521 aattcaaaaa tatagagcaa aacaaccgca atatcctttt atatagggac aacaatgtaa
     5581 acggtctgaa agccggacac acagaaagcg gcggctacaa ccttgccgtg tcatactccg
     5641 gcaacggcag gcacatcctt gtcatcacat tgggttcgga atcggcggaa acacgcgcat
     5701 cagacaacag caagctgctg aactgggcat tgcaggcctt cgatacgccc aaaatatatc
     5761 cgaaaggcaa aaccgttgcc caaatccaaa tttccggagg cagcaaaaaa accgtccgcg
     5821 caggcttcct caaagaagcc tacatcactc tgccacataa ggaagcgaaa atggcagaac
     5881 agattttgga aaccatacag ccgattcccg ccccggtaaa aaaagggcaa attttaggaa
     5941 aaatcaaaat cagacaaaac ggatacacca ttgccgaaaa agaaatcgtc gcactggaaa
     6001 atgtaaaaaa aagaagccgg tggcaaaggc tttgggcgcg tctgacaggg cagtaatctg
     6061 ctatttcaaa tatcccgttt ttccaacaaa taaagaaatg ccgtctgaaa cacggttcag
     6121 acggcataaa acaacagggc ggtacgtatt gcatacgcgc cgccctgctg ctgaaatcaa
     6181 ttagcgtttc ttaccggtaa cggtagcaac agccagattt tcgttacgtt tcagggaaac
     6241 gctttctaca ccttcaggca gtttgatgtc tgacaagtgc agaatgtcgc cggcaaccac
     6301 ttcagcacaa tccaaatcca agaaagcagg gatgttggca ggcaaagcaa ctacttcaac
     6361 agaagtgttt aacagagata cgcggccgcc ttgcagtttg accgcttggg aattttcagc
     6421 gttaacgatg tgcaggggaa cgcggatgcg tacgggttgg tctgctttca cagcttggaa
     6481 gtcgatgtgt tgaacttcgc ggcggaacgg gtgcatttgg aaatcgcgga cgataacgtc
     6541 tttggtttca ccgttcagag acaacttaat caacgcagta tggaaagatt ctttttccaa
     6601 tgcgtagaat acggttttgt gatccacagc gattgcaaca ggctcttgac cttcaccgta
     6661 cagaatgccg gggatttggc cttcgcgacg caggcggcgg ctcgcaccag tgccttgtgc
     6721 ttcacgaaca gaggcttgaa tttcataagt catgttaaat actccaagtt aggtaaaatc
     6781 gccgtcatcg gccgcgacca gcttaagacg gcttcgggct tatggcagca acatgctgcc
     6841 tgtcatcact tcttcattga aaagatatga gacggattct tcattgctaa tgcggcggac
     6901 ggtttcggcc aacagaccgg caatcgttac ctgacggata cggtcgcagt ttttagccgc
     6961 ttcagacaaa ggaatggtat cggttacgac cacctggtcg atttcggatg aggcgatacg
     7021 gctgaccgcc tctccggaga atacggcgtg gctggcatag gctagaacac gttcagcccc
     7081 ccgctctttc agggcgacgg cggctttgca cagcgtattt gcagtgtcaa tcatatcgtc
     7141 cacaatcaga caggttctac cttgaatatc gccgatgatg ttcatgactt ccgccacatt
     7201 ggctttcggg cggcgtttgt cgatgattgc caagtcggca ttcagggatt ttgccacggc
     7261 gcgggcgcgg acgacaccgc cgatgtccgg gctgacgacg gtcagatttt caatccgctg
     7321 ttgtttgatg tcgttcaaca gaatcggggt ggcataaata ttgtccaccg gaatatcgaa
     7381 gaaaccttga atctggtcgg catgcaaatc gacagtcaaa acacggtcga tccctgccga
     7441 atacagcata tttgccacca gtttggcaga aatcggaacg cggacggaac gcggacggcg
     7501 gtcttggcgc gcatagccga aatacggaat ggctgtggta atacgacctg ccgaagcacg
     7561 cttcagtgca tccgccatcg tcaggatttc catcaggttg tcattggtcg gcgcacaggt
     7621 cggctgaagg atgaaaacat cgcgcccgcg tacgttttcc aacagttcga cggcaacttc
     7681 gccgtctgaa aacttggata cggaagcatt gcccaaagaa atgtccaaat gcctgacaac
     7741 acgttgtgcc aattcgggat tggcattgcc tgtaaatacc atcaaactgt cgtacgcagc
     7801 catattctca cctgatttta tgtttaactt ccgctcagaa aacacaatgc ttccctctgc
     7861 ttgtaccaag cccgacatta tacctgcaac atccgacaca aacaaaggca tttcaatatt
     7921 tttatttcta tgaaataaaa gcgtgtaagc aggcttacac gcttttattt ggctggggag
     7981 gaaggattcg aaccttcgca tgctggaatc aaaatccagt gtcttaaccg cttgacgact
     8041 ccccaaaaag ggctggctgg ggaggaagga ttcgaacctt cgcatgctgg aatcaaaatc
     8101 cagtgtctta accgcttgac gactccccaa ctcgtttggc ttggctgggg aggaaggatt
     8161 cgaaccttcg catgctggaa tcaaaatcca gtgtcttaac cgcttgacga ctccccaaca
     8221 atgtttatac ggacaacaaa ggatgttttg aaagaccctc tgccaaatat gcctcgtaca
     8281 aacctgaaac ttgtcggtat atattgtatg cgctatccct atcttgacac gccgtgaata
     8341 cacacgcacc agaacctgtc attaaggcaa atccatatcg ggacaattcg gaataggctt
     8401 tccaaacttc agggtattct ttaaatacaa ctgcctgcat atcatttcta aacggttgca
     8461 ggttttggaa agtcggcatt atgcttgagg cggaatttcg tgtcaagcct tcgtgtgtga
     8521 aaatttttgc agtggaaacg tggacgggcg gtttgacgat gacataccac tgtttcggaa
     8581 tatccattcc tatcagtttt tcacctatcc cgcttgcaaa agcatttttt ccgaaaataa
     8641 aaaatggtac gtccgcaccc aaagttgcgc ccaagtcaat cagctgctgc gtcgttcggg
     8701 aacactgcca ccaacggttc aacaccagca aaacggttgc cgcatccgag cttccgccgc
     8761 ccaaacccgc ccctgtcggg atttttttgt ccagccatat ttccacgccg gcggggttgc
     8821 gcgcatattt ttgcagcaac gatgcggcac ggtagctcaa atcggcttcc tgcggcatgc
     8881 catcaaccgg attgtgcagg atgattttgc cgtcgtccct cggtttcaaa tatacggtat
     8941 cctgcaaatc tatcaagcag aatatgcttt cgatattgtg ataaccgtct tcccgcctgc
     9001 cggtaatcct caaatcgaga ttcagttttg caggtgcggg aaacgcctgc cgtccgtccg
     9061 caatattcat ctgtccgcct tatctcgtgc gcgccgcaca gcgttccggg gtttcggttt
     9121 cagacggaat accgatttcg gtgaaaacca gcctgatgtt caaatttccg ttattcagtt
     9181 gcaacgttcg gacttgcccc ccactgtcgg cggttctgcc gacagtccaa ccgtattgtt
     9241 ccaatatgcc gtccggcagg atgcggtaag gcgcgcccgc cacacgcctg ccatctgccc
     9301 agatatgcag atattggatt ggcagtttga aaccgaccag ctgcctgctc aattcttccg
     9361 cactttccgc ctgatagaca tttcctttgc cgtccactgc caatgcgccg tccctgtctt
     9421 gacacaactg cccgagcgta ctgcccaaag gggtattgat attgatggtt tccacgagcg
     9481 gttggtatgt ccaatcgaaa tttgcatacg aacctttccc ttccgctttc actgccaacc
     9541 gcccttctgc tgcaaaactg ctgatgtgtt cggacggctg ccacaggttt tcgttatttt
     9601 gaggtaattg cgcacaagcg gtcaaaagca ggatgaccga tgcggatacg gtgtgtttca
     9661 tcagaatttc cttaacgggt gccatcctgc cggccggcgg gttcggcgtg ccgtgcaaaa
     9721 cgcgtgcgaa ctgcgtgcgg tttgcccgaa tgccgcagct tcccggcaaa ccttatgccg
     9781 tctgaaagga tggacctgca ttatttccgg ggtttgcggg aaggttgggg caatgcgatg
     9841 ccgtgacgtt tgagcgtttc ccgccatatt ttcttgtctc ccgtaaggtg tgccgcctgc
     9901 gtccatacgt caaccgcctg atcgcgttcg cccaatgccc acaacacttc gcccaaatgg
     9961 gcggcaactt cgggctcggg gtcgttttca aacgaatacc gcagatacgg cagcgcgctt
    10021 tccgcgtcgc ctttcaggta atacgcccag cctatgctgt cgttgacagc ggtatcgtcc
    10081 gggttgattt ggtatgccgt ctgaagcagg gcgaagcctt cgtccaaacg tttggaatcg
    10141 gaaagcaggc tgtagcccag attattcata atctgagcgt tatcgggtgc aagcctgaac
    10201 gccctttcaa gatctgaaat catttttttc cgcttgccaa gccgatcgta aacaactgac
    10261 cgctgtacca atgcctctgc ctgtaactct gtattactgc cggcaggcgg tttttcgata
    10321 atcttgtcca accccctcaa agcctcccgt ttgtcgggca gcttcgacag ggcgaacatc
    10381 tgtattttgg acaaattgtc tgccgtaaaa taccgcccct gctgttcggg aagtttccgc
    10441 accctgccga tctgccgcaa agccgccctg ccgccgtcca actcgacagc cgccgcagcc
    10501 gccagcacac ctttgtcgaa caggtattcc ggagcggaca cttttttcaa ccactgcctg
    10561 actttggtgt aatcccttcg gtcggcatat atcatcgccg ccgtcattgc cgccctgccc
    10621 cgctgttccc ccgtccccct gccgtatgcc ttttcggcgt agccgtcgat aacggaagca
    10681 ccttcttttc ggtttgccgc caatatcgct gcctgaatat acaggtctgc attcggattg
    10741 cgttccaaca gcacgttcaa acgcgcatag gcatcatcca gcctgtgcag ggaaaccaga
    10801 ttcataattt ccatttcctg ccagacggcc gaaaggtttt gggtgtctgt ctgctcgaaa
    10861 aagccgtcga gtatttcggg atatttgcgt gcagtcagac gcaacgtcat taaagtgggg
    10921 ggcaatattt ccgtatcgag cttcgccaaa cgctgcaaag ctccgattgc cttttccttt
    10981 tcgcgtccct gtacgctgaa caccacatcg gcaaccgccg cttcgggcag atgttcatat
    11041 ttcaacgccg cacggcgaac cgctttcgat gctttttgcg ccaacccgtc ctgttgcacg
    11101 gcggcttgtg ccaacaataa aaacaccctg cggttctgtc cttcgtccgc ctgagccagc
    11161 acttcttcca gtccgtctag atgctgattt cctctttccc tcagcacgtt ccgcagccac
    11221 cccgcccgtt tttgcgcctt acccggtata ggctcaatct gccgccattt ctgataaatc
    11281 atttccgcct gttcaaacgc gttcagcgac acggccattt ccaaggcgcg ttcggcgact
    11341 tcgggggatt ttgtgcgttc caacatcagc atataggttg ccagagccgt tcccgcctgc
    11401 cccttttgca aggcggtttc ccctcccagc aacgtaaata tctgattaac ccgctcgccc
    11461 actgccgcaa gccgtgcgcg ttcgtttttg atttcttcct cgctgtaacg ctgctgcttt
    11521 ctgaaaacct ttccgacttc cttcggcggc ttcgcatccg ccgcgccggc ggcatacgcc
    11581 tgcccggcaa gcagggctgc cgcgagcaca gataaaatgg tgaaacgggc gggtaacata
    11641 atcttccttt atccgacagc cgtccgaacc ttcagacggc atataatcag gcgtaacttt
    11701 atcacaagca gaatcgtttg tagatttgcg aaaccccgtt caaaaaaata aatgccgtct
    11761 gaaacgcaaa tccgcttcag acggcattcc cgacgcttgc ggtttaaggg agttttattt
    11821 ccaaattgtc aatcaggcgc gtcgtcccca gacgggcggc ggccaagacc accagtttct
    11881 tatctcccgc ccgcgccact tcgagcgtat cggcgcggcg gatttcgaca taatcgacca
    11941 cccagcccgc agcggtcaaa tggtcggcag cctgccgctc caaacctgca taatccaaac
    12001 tgccctgcgc caaggattcg gcaacagcct ttaattcgcg gtacaggcgc ggtgcttcgt
    12061 cgcgttccgc cgcactcaaa tactggttgc ggctggagag tgccaaaccg tcttccgcgc
    12121 gccccgtatc gacaggcact atttcaacat caaaattcaa atcctcggtc aaacctttaa
    12181 tcacggcaag ctgctgatag tcttttttgc cgaaacaggc aacatccggc gcgacaatat
    12241 tgaacagctt ggacaccacc gtcgccaccc cgcggaaatg tcccggacga aacttgccgc
    12301 acaactcatt ttgcagattg ggcggttcga cgttgtaacg ctgttccacg ttcggataga
    12361 gttctttctc atcgggcgcg aaaacaacgg caacaccctc ggcttccagc ttgtccgcat
    12421 cctgttgcaa agtacgcgga tatttgtcga aatcctcgcc ctgaccgaat tgcaggcggt
    12481 tgacgaatat gctgacaaca acgttgtcgg cgcgtttttt ggcttcgcga acgagggcaa
    12541 gatgcccttc gtgcagattg cccatggtcg gcacaaatgc caccgttccc gcatttttac
    12601 gccacgcgcg cagttcttga atggtatgta tgatttgcat aacggtaatc cttatccttc
    12661 gggcttcctg cccgacaaaa cgcgccgatt atacgcgccg cgcacggcaa aacaaaatgc
    12721 cgtctgaaac ggctttcaga cggcatcggc agcaaacctt gttcagtctg caaaaatatg
    12781 ttccgcagca gggaaggttt tggctttgac ttcggcaaca tacgcccgaa ccgccgcctg
    12841 aatgctgctt tgcccctgca taaagttttt gacgaatttc accgtcttac ccgggaaaat
    12901 gccgagcata tcgtgcatca ccaaaacctg cccgtcgcaa tcgacacccg cgccgatgcc
    12961 gatggtcgga caggaaacgg attcagttac cttttttgcc agttccgcag gaacacactc
    13021 catcagcacg accgccgccc ccgcatcatc atgcgccttg gcatcgttaa gcaacgcctg
    13081 cgccttgccg ccgcgcccct gaaccttgta tccgccgaag gcaaacacgg attgcggggt
    13141 cagaccgatg tgggcacaga ccggaatacc gcgcatttgc agaaattcgg tcgtttccgc
    13201 catccacaca ccgccttcga gtttgaccat atgcgcgccg gcagccatca gttcggcggc
    13261 ggcggcaaac gcctgctcct tactctgctg atatgcacca aacggcaaat cgctgacaat
    13321 catcgcattt tttgcaccgc gtgctacaca ttcggtgtga taacacatat cgcgcaggct
    13381 gacgggcagc gtcgactgcc gcccttgaac cgccattccc aaagaatccc caaccagcag
    13441 cacatccacg ccggcatcgt ccatcagcgc ggcaaaactg gattcgtaag cggtcagcat
    13501 agcgattttc tcgcccgccg ccttcatttt ttgcagtgtg ttcacagtaa tcatcagata
    13561 ttgttacccc gcccttttca gacggccttt tcagtttgaa acgggctatt atagtgaaat
    13621 gcccgcagcc ctgccaccga ccgtgccgca aatgcaaaac agcccgactg ttttacacaa
    13681 tcggactgtc aaatctggtg ccggcaccaa gagtcgaact cgggaccccc tgattacaag
    13741 tcaggtgctc taccaactga gctataccgg cttactgaaa tgttttgcaa caccatcagc
    13801 agaaatggtg ccggcaccaa gagtcgaact cgggaccccc tgattacaag tcaggtgctc
    13861 taccaactga gctataccgg caaagaaggc gaattatgca ggcaacttca cactttggca
    13921 aggaatttat ccgaaagccc cggcaaatcc gaataagtaa tttatttgaa aagaaattaa
    13981 tttgaagcag gacaagcatc ggcattctga taaaatatcc gcctttcaaa ccgaccgttc
    14041 acaccgatgg caagacatcc ctaccgccgc ctgcgtcctg caaaatccgg ctttcccgaa
    14101 gtcggcattt ccgaagaagg caatatccgt tcgcttcact tgggcagcga caccgttcaa
    14161 agctcaatga acctcgacca cccgtccgag ctggtactct cttacagccg cgcgatgatg
    14221 ggctggctgc tgtttaccga cgcacttccg cagcatatca cccaaatcgg cttgggcggc
    14281 ggctcgtttg cacgctggat agacacctac ctgcccgaca cgcgccaaac cgccgtggac
    14341 atcaatcctc aggtcatcgc cattgcccgc aacctgtttg agttgccttt cgagggcgag
    14401 aaatttgaaa ttattgaagc agacggtgca gaatatatca aagtcttccg ccacaacacc
    14461 gatgtgattt tggtggacgg gttcgacggc gaacaaatca tcgatgcgct ggttgaagaa
    14521 ccgtttttcc gagactgccg aaacgcactc tcttcagacg gcatattcgt aaccaactgg
    14581 tggagcggcg acaaacgcta ccaacgcttc atcgaacggt tgttgagcgt ttttgaaggg
    14641 cgcgtcttgg aacttcctgc cgaaagccac ggcaatgtcg cggtaatggc cttccaaagc
    14701 agccccaaag agcaaaacat agacaaactc aaaaaacgtg ccgacaaact gagcaacgca
    14761 tacggattgg acttccaccg tatgcttgcc ggcctgaaag cgtccaaccc caacaacggc
    14821 aagcatttcc acctttaagc aactatcttc acggctgcat tggaacatgc ggaaaatcaa
    14881 gaggaaatca ttatgctgaa acccgaaacc atacacatat ccggtcctgc cggcatttta
    14941 gaaaccatcc atatcccgtc cgaacaagta ccggcacgcg gtgttgccgt catcaatcat
    15001 cccaaccccc tccagggcgg aacaaacacc aacaaagtta tccaaactgc cgccaaagcc
    15061 ttaagcaaac tcggcttcca ctgctaccta cccaaccttc gcggcgtagg cggcagcgaa
    15121 ggcacacacg attacggacg cggcgaaacg caagactgcc tcgccgtcat cgactatgcc
    15181 cgtgcccaac atcccgaagc ccccgaattt gccttatccg gcttctcctt cggcggttat
    15241 gtcgccacat ttgccgcaca agcgcgcata cccgatttat tgctgctcat cggcgcggca
    15301 gtctgccact ataccggccg ccccgagccg tccgccgttc ccaatgttgc caaaacgctg
    15361 atgatacacg gcgcggaaga cgaagtcgtc gaaatcggga aagcactgaa atgggcggaa
    15421 ccgcaagatt tgcccgtcat taccatcgcc ggttccacgc atttctttca cggcaaactc
    15481 atcgtcctgc gcgacaccat cctccgcttc gcgcccgtat gcctgaacgg ataaaacccc
    15541 tttcagacgg catcgataat gtaaaacccg tatcggcggc atccgccgat acgggtttgc
    15601 gttccctctc attataggca aaatccgctg caaaacaccc tttcccgacc cgacaaagga
    15661 tctgcaaacg caatcttttt tgccaatagc ttcaaaggtt tccgataatc ctcgctgccc
    15721 gcttcagacg gcacgggata gagcgcgtca ttcaacagcg gcatacccaa acccatcata
    15781 tgcacgcgca actggtgttt cttgcccgta tgcggcgtaa ggcggtaaag gctgaattcc
    15841 cccctgtttt caatcagttc gaccgtcgta tgtgcgtttg gttcgccttc cgcctcttgc
    15901 gtcgtaaaaa atttctcacc cctcaccaaa cgcgaaacca catcgagcgg atacggcaaa
    15961 tccgtccttg tcggcgcaag cgcctcatac gttttccata ccgttttgtt ttgaaacatc
    16021 gtctgatagg ctccgcgcgt ggcaggatta tgcgacagca gcatcacgcc tgccgtatcc
    16081 ttgtccaagc ggtgcagcgg cgtaatgtcc tcaacattca aatgctgcaa ttcaggccgc
    16141 aaacgcaggc gcgtgagcag ggtttcccgc aaaaacctgc cgctggggat gacgggcaga
    16201 aaatgcggct tgtccaccac aatcaaatgc tcatcaatat gcaaaatctt ttcttcaaac
    16261 ggaatacgcg gctcatcctc actgctggtt tcccgataat aaaacatcac cttacccggc
    16321 tcgaacaaag tatgttcgtc caacgtcgca ccatccgaac cgaccacaaa accgctgttc
    16381 aaccgcctgc gccaatcgtc cgcgcccaca aaaggaaagc ggatgcacag aaaatgcagc
    16441 agcggcagcc cgtaaaactg cttttcatgc ggcagcacca aataactggg tttgacaccg
    16501 ttcaacagag gaagaggatt attgcgtttt ttcatgcggc ggattgtaac cgtttttcac
    16561 aaacttttca gacggcatat cgaaacataa ttgacaaaaa caaacatatt gttttttatg
    16621 atttaaaaat ttatttagac aagattatcg gatattcatt attatcagta aataagaatt
    16681 aatatcaata ggagaaatat gaagcgcatc tttttgcccg ccttgcccgc catcctgcct
    16741 ttatccactt atgccgacct gcccttgacg attgaagaca taatgaccga caagggcaaa
    16801 tggaaactgg aaacttccct tacctacctg aacagcgaaa acagccgcgc cgaacttgcc
    16861 gcaccggttt acattcaaac cggcgcaacc tcgtttatcc ccattccgac cgaaattcaa
    16921 gaaaacggca gcaataccga tatgctcgcc ggcacgctcg gtttgcgcta cggactgacc
    16981 ggcaataccg acatttacgg cagcggcagc tatctgtggc acgaagaacg caaactcgac
    17041 ggcaacggca aaacccgcaa caaacggatg tccgacatat ccgccggcat cagccacacc
    17101 ttcctcaaag acgacaaaaa ccccgcccta atcggctttc ttgaaagcac ggtttacgaa
    17161 aaatcgcgca acaaagcctc gtcgggaaaa tcgtggctca tcggtgccac cacctacaaa
    17221 gccatcgacc ccgtcgtcct ctcattgact gctgcctacc gtatcaacgg cagcaaaacc
    17281 ctttcaagca acaccaaata caaagcaggc aattactgga tgctgaatcc caatatatcc
    17341 ttcgccgcca acgacagaat cagcctcacg ggcggcatcc aatggctggg caagcagccc
    17401 gaccgtctgg acggcaaaaa agaatccgca agaaacacat ccacctatgc ccatttcggc
    17461 gcaggtttcg gtttcaccaa aaccacggct ttaaacgcat ccgcacgttt caacgtttca
    17521 gggcaaagca gttccgaact gaaatttggc gtacagcata cattttaagc aggttttgat
    17581 tttccaacct tgtaatccaa aggagttatt atgaaaaaac aaatcacctc agccgtaatg
    17641 atgctgtcta tgattgcccc cgcaatggca aacggattgg acaatcaggc atttgaaaaa
    17701 tacgtattcc acacgcaggc agatgcgccg atgcagttgg cggagctttc tcaaaaggag
    17761 atgaaggaga ctgagggggc gtttcttcct attgtggcag gtgctttgat gggaggggca
    17821 attagtgctt gggctaatca cggcatttct aaaattaaaa cagggcaatt tgcttcaaca
    17881 agaagtacct tggctgcaac cgctggtggt atgataggag gcggatatag cagtgcgatg
    17941 ttgcgcggtg cagggattac tactagtgtt tttgcccgtt ctgcttggaa agggggaaat
    18001 gctattccta atgcagttat tcgtgcaaat ggtgcagcat taaatcagtc ttctagcgcc
    18061 gcaatgggaa aacatagaag ataaacgaaa tggatgatct aatactatat tttttatcag
    18121 gtatattcgg aaatcaagtt gctgaatata ttataaaaaa caaccgtgag ataaaagttc
    18181 cctttattgt gctttacgca atatttttta cgttgattta tactgtggct ttgttgtttc
    18241 tcagtctgat ctattgggtc aatggtgcgg agattgcatg gaaggggata ggtattttca
    18301 gtatgtcggt cagtttttgt atagtcttct gtctttattt gattgacaag gcaggaagat
    18361 gtaaggataa gaaacaatag ggatcagatg cggcggcaaa cgtagattgg gtctaggctc
    18421 aacctgcgtt tgctgcatct ttggaagaaa tagtggattc gaaaaaatag gaagaattct
    18481 gattaatggt atgcgaggtg tagcaatggg ggctgttgct ggtggcataa atggttatgc
    18541 aggttctacg ggtaagaaca ctgacattcg ccgttaaata ataaataaac caacttccaa
    18601 ccaaacctgc tatcggtttg gttggaagtg agaggaggta aggtagtgtt tgctgttatt
    18661 ttcttttcga cattgggctg tattttagca tggataagag atattcctaa aataaaatca
    18721 aaaaaaattt tggcaagatc cttatatatt ataggaataa taaatgtaat aatcggctat
    18781 gtattaatta aaaatatatt ggtttctgtt tcggatgaag atggaacaaa atatcttgca
    18841 atatatttat caaatctttt ttttgggaca gtattgatgt atgttcttgt gaaaagatta
    18901 tctaaaaagc caagctgaaa cccagtacac aactcatcaa taaaaatccc ttttgcacgg
    18961 aacaatgaca atattcaaat tgctggattt gcaatctgat ttacctattg aaattgggtg
    19021 tgcaacatac gttctaagca gggctttgat tttccaacct tgtaatccaa aggagttatt
    19081 atgaaaaaac aaattaccgc agcgatgatg attctgacaa tgattgcccc cgcaatggca
    19141 aacagcatgg acaatcagat atttgaaaac caagtattcc atacgcgggc agatgcgccg
    19201 atgcagttgg cggaactttc tcaaaaggag atgaaggaga ctgagagggc gtttcttcca
    19261 ttggctatct tgggtggtgc tgccattggt atgtggacac agcatggttt tagttatgca
    19321 acgacaggca gaccagcttc tgttagagat gttgctattg ctggcggatt aggcgcaatt
    19381 cctggtggtg taggtgccgc aggaaaggtt gtttcctttg ctaaatatgg acgtgagatt
    19441 aaaatcggca ataatatgcg gatagcccct ttcggtaata gaacaggtca tcctattgga
    19501 aaatttcccc attatcatcg tcgagttacg gataatacgg gcaagacttt gcctggacag
    19561 ggaattggtc gtcatcgccc ttgggaatca aaatctacgg acagatcatg gaaaaaccgc
    19621 ttctaactcc tccttttttt ctatttgaag atgtgtcttt ggatatattt caaggattgt
    19681 cggaattgga aaagaaaata gagccgcagg atttgatgga tgatgtttac cgtgcttttg
    19741 attcggttgg aaatattctg aatttccgta tagtagaaaa agagcaaaaa gggttttggg
    19801 taagtaccaa aatcaaaaca gttgtatttg attcggcaga tatgtcttct gatgatcttt
    19861 ttctgaaatg cttacagtct tcatataagg cttattttga aactgaacct gccggtttag
    19921 ataaacggca gctgatgaaa acgttaattc aaaaatgcgg attcagttgt tgacgaaaaa
    19981 atggattctg aactaacaga aactgatttc gaccatgtat gaggtaaaaa atgtctttcg
    20041 gctatttgat tgcaacatcg caaccttgcg aattgttgac aaaatctcga ggtgaaactt
    20101 tttctttaat tatggataag atggatttat ggatttattt cagattttgt gaaggaaata
    20161 tttatacaat aaggaagaat gaaactgaat cttgtcttac agaaagagga ggcaaatggt
    20221 tgaaacatat ttatgaattt aatcggggaa gttttatttt ttcttatgtg cttctaaaaa
    20281 agagagaaag cgaagagaat tttgcagaaa ttgttttaaa atcaataaaa aataataaaa
    20341 ttttgacagt taaggtacgg tcaggcttgc attttgactt aagaaatatt tatcgtattg
    20401 agatgtatag
//
LOCUS       DAWFAD010000021        20380 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWFAD010000021 DAWFAD010000000
VERSION     DAWFAD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.021"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            238..354
                     /locus_tag="WGO49_000571"
     CDS             238..354
                     /locus_tag="WGO49_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ0305714.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELGV"
     gene            complement(697..1212)
                     /locus_tag="WGO49_000572"
     CDS             complement(697..1212)
                     /locus_tag="WGO49_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305715.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CHSTDLPPIGNYSSERYIPPQTPEPVSSPSNGGQVVKYKAPVKTVSKPAKSNTPPPQQ
                     APSNNSRRSILETELSNERKALVEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(1278..1691)
                     /locus_tag="WGO49_000573"
     CDS             complement(1278..1691)
                     /locus_tag="WGO49_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0305716.1"
                     /translation="MPSDKEARAGCLSLPANNGIIRALSPPFTGINIRLFAALGLLSL
                     SGAAAQASVYHCNSNGKSVYTSAPAGSCADADLPKISSHQGGGYRLKIKKLSQEAKIH
                     IESKKKNKKPAGKKNRQAAKAPKENQKQPTEKKSP"
     gene            complement(1678..3600)
                     /locus_tag="WGO49_000574"
     CDS             complement(1678..3600)
                     /locus_tag="WGO49_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0305717.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPKVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMVQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSQLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(3677..4072)
                     /locus_tag="WGO49_000575"
     CDS             complement(3677..4072)
                     /locus_tag="WGO49_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305718.1"
                     /translation="MKRQQDKKMLFEENPIDEHFAEYDRRTGEIRLSGRHFNKPVLLH
                     RDSAGLSKWQSLSELTPDNLLSDVKADDYPEILIIGTGAAQEFIHPKIMADFSRIGIG
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            4246..5553
                     /locus_tag="WGO49_000576"
     CDS             4246..5553
                     /locus_tag="WGO49_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ0305719.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREVRISAVCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGHDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            5546..5980
                     /locus_tag="WGO49_000577"
     CDS             5546..5980
                     /locus_tag="WGO49_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305720.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLTVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(6039..6308)
                     /locus_tag="WGO49_000578"
     CDS             complement(6039..6308)
                     /locus_tag="WGO49_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0305721.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(6490..8940)
                     /gene="lon"
                     /locus_tag="WGO49_000579"
     CDS             complement(6490..8940)
                     /gene="lon"
                     /locus_tag="WGO49_000579"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ0305722.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHIHVP
                     EGATPKDGPSAGIAMTLAAVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            9191..10294
                     /locus_tag="WGO49_000580"
     CDS             9191..10294
                     /locus_tag="WGO49_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0305723.1"
                     /translation="MSSFIFPDNGAEWRNESVQKPPKGWHYVRESGADGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRARKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLGLRPADFLEKAFIRAGLRVADMM
                     KTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
     gene            complement(10322..12067)
                     /gene="recD"
                     /locus_tag="WGO49_000581"
     CDS             complement(10322..12067)
                     /gene="recD"
                     /locus_tag="WGO49_000581"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ0305724.1"
                     /translation="MERQTDEFAQAAARAAIWFLARYAGSGSEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQPFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGEAHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNKAYCSHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPDFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEETFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(12135..12830)
                     /gene="lolD"
                     /locus_tag="WGO49_000582"
     CDS             complement(12135..12830)
                     /gene="lolD"
                     /locus_tag="WGO49_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ0305725.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGILRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKKREEAAETAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(12823..14070)
                     /locus_tag="WGO49_000583"
     CDS             complement(12823..14070)
                     /locus_tag="WGO49_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ0305726.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            14275..14553
                     /locus_tag="WGO49_000584"
     CDS             14275..14553
                     /locus_tag="WGO49_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305727.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(14609..15226)
                     /gene="recR"
                     /locus_tag="WGO49_000585"
     CDS             complement(14609..15226)
                     /gene="recR"
                     /locus_tag="WGO49_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ0305728.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(15293..16831)
                     /locus_tag="WGO49_000586"
     CDS             complement(15293..16831)
                     /locus_tag="WGO49_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ0305729.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAMQNEQADGGSPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPKAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPANEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIGLP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(16886..17260)
                     /locus_tag="WGO49_000587"
     CDS             complement(16886..17260)
                     /locus_tag="WGO49_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ0305730.1"
                     /translation="MIILHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQRPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAGTQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGRV
                     YVGFSEETYDGIFNRQALCRQG"
     gene            17410..19038
                     /locus_tag="WGO49_000588"
     CDS             17410..19038
                     /locus_tag="WGO49_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ0305731.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQDGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            19110..20363
                     /locus_tag="WGO49_000589"
     CDS             19110..20363
                     /locus_tag="WGO49_000589"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ0305732.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYTLARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWFALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKTFRAAQDKTEKH"
BASE COUNT         4554 a         5490 c         5365 g         4971 t
ORIGIN      
        1 atgaagcaaa aacgccttaa agtccaactt aagctgctca ttgatgaaaa agggctggat
       61 attgatgcag tgtttgagct gattatcagg cacgcggaat acggagctta atatgaaaat
      121 cggcaatcta aatgtagaaa tccgcaggaa agcgattaaa aacctgcaca tcagcgtgtt
      181 gccgccaagt ggcgcggttc gggtgtccgc accgcttaat acgtcgtcgt ccacgaactg
      241 acccatttgc tcgaacgcca tcgcaacgcc cgttttatgg cgcatatgga caactttctc
      301 ccaaactggc aaagcatcaa acaacagctt aatgccttgg agttaggggt gtaacgcaag
      361 gacgaatccg aagcagaccg tttgcacaaa tatataattt aacataatat acattatgcg
      421 aactatcgga aacagttgta cgtgtccctg tggtctttcc aagtaggaaa attaaagtat
      481 ggccaatgcg gctgaatgta tagcccggag catccgcgta tccgaaggta ctgaacgaca
      541 tgccctgcat ccctttcgac gcttccatcc agccggtatt gggaaatacg ttgaccttca
      601 atataatata caggcagccg acacacagga tatgccgctg ctttttcatc atttcttctg
      661 tcaaatcctt ggaacggtcg atttgaaaac acggctttac atacgcccca gttccctttg
      721 cagggcttga atattttgct gcctgtccaa tacattgctt tgtaatgcat ttatttcttg
      781 atggttgatg ttgccgccct ttgccagacg tgcttgtgat aacatttttt gggcttcaac
      841 caatgctttg cgttcgttgc tcaattctgt ttcgagaatg gagcgtctgc tgttgtttga
      901 gggtgcttgt tgcggcggcg gcgtattgga ttttgccggc ttggatactg ttttgaccgg
      961 ggctttatat ttgacaacct gtccgccgtt tgacggtgat gataccggtt cgggcgtttg
     1021 gggcgggata tagcgttcgc tgctgtagtt gccgattggg ggcaaatcgg ttgagtggca
     1081 gcttttggac ggcttggtgg tgtaaacggt ttctccgttg attgtgcagg tgtagatttt
     1141 ggccgcattc gcacccaatg ggcttgaaat cagggaaaag ttgattagga ttaaggggag
     1201 ttttgatttc ataatgtgct tgattttcgg ataatcattt gattttttgg tattttttat
     1261 aatgctttct tggcgtttta cgggcttttc ttttcggttg gttgcttttg attttctttc
     1321 ggggctttgg cagcctgcct gttctttttc ccggcaggtt ttttgttttt ctttttactt
     1381 tctatgtgta ttttggcttc ttgactgagt tttttaattt tcaggcggta tccgcctccc
     1441 tgatggctgc tgattttagg taaatccgca tcggcgcacg atcctgctgg ggctgatgta
     1501 tatacgcttt tgccatttga gttgcaatgg tatacggagg cttgcgccgc cgcgccggaa
     1561 agggacagga gacccaaggc ggcaaaaagt ctgatgttta taccggtaaa aggcggtgat
     1621 aacgcccgaa ttataccgtt attggcaggc aaagataagc accctgcccg cgcttcttta
     1681 tcgctcggca aactgtttct cgatttcagt ttcaatccgt tccaattctt cttgacaagc
     1741 cagccaaacc tcttcgattt gggcaagttg tgttttgact tttgccagct gggataaggt
     1801 attttgcaat ttttctttgt ttgcttcaag gtaagcgtct tcttgtgcta aaaatgcttc
     1861 acatgccgtc tgaatttcgg aaagctgcac catttctttt tcggcacggt ctattttctg
     1921 ctgtatcggc ttgccgcgtc gggctttttc ctgacggatt tgcgcttcga tgcgcttggt
     1981 gtctttgcgg ctttggcttt gtgcggatgc tgcgggtgcg gcggcggcgt tttcctgtgc
     2041 caaacgccat tggcggtaat cgttcaaatc gccgtcgaag ttcttcagac ggcctttgtc
     2101 gatcaggagg aagctgtcgg tagtcgcttc aagcaggctg cggtcgtgcg atacgatgat
     2161 taaggcgcct tggaaacttt gcagagcgag cgtcagggcg tggcgcatat ccaagtccag
     2221 atggttggtt ggttcgtcaa gcagcaacag gttcggcttt tgccagataa tcatggcaag
     2281 ggcaaggcgg gctttttctc cgccggaaaa aggttcggtt ttctgtaacg ccatatcgcc
     2341 aacaaaattg aagccgccga ggaaatttcg gatttcttgt tcgcgtactt tgggagaaag
     2401 ctgctgaata tgccaaacag ggttttggtc ggcgcggatg gtgtcgagtt ggtgttgggc
     2461 aaaatagccg atattgagtt tttcggaacg gacgatgctg ccggaaagca aatcgatttt
     2521 gcccgccaag gctttgataa aggtagattt accgctgccg ttgacaccca atagcccgta
     2581 gcgtgcgccg ctttccagcg acagggtaat gtcatgcaga acggttttgc cttcgtaacc
     2641 caaatcagcg tgttctagct ttaacaaagg attgggcaga tggtcgggat ggtaaaactc
     2701 aaaggaaaac tcgctgtcca gatgcgcggg cgcaacgcgt tctagctttg ccaaagcctt
     2761 catgcggctt tgcgcttgaa cggctttggt ggctttggct ttgaagcggt cgataaagga
     2821 ttgcagatgt ttgatttgcg cctgctgttt gacataggca gcttgttgtt gcgcgagacg
     2881 ctgcgcgcgt tcgttttggt aaaaatcgta attgccgccg tattgcgtga gtttttgctg
     2941 cgataattca atggtttggg tagttgccgc gttgagaaaa tcgcggtcgt gggaaatgat
     3001 gatttgcgtg cagggtaaag aggcaaggtg gttttccagc cacaagacgg tttctaaatc
     3061 caagtggttg gtcggttcgt caagcaagag caaatcggcg cggcaaatca gggcttgcgc
     3121 aagattcaag cgcatacgcc agccgccgga aaaggatttg acggggcggc tgtgttcttc
     3181 ttgcgaaaaa cccagcccgt tcaacaattt tgccgcgcgg gccggcgcgg tataagcgtc
     3241 gatttcttcc aatttggcat gatattccgc ctgcttcatg ccgtcatttt gcgcttccgc
     3301 ctgcctcaat gccgtctgaa aagcctgcaa ctcggcatcg ccctgcaaaa cgtaatccaa
     3361 agcggaaata tccaaatcgg gcgtttcttg ggaaacggaa gcgagccgcc agtttttcgg
     3421 aatcgagaca tcgccgccgt cctgagtgat tttacccttg attaaggcaa acaggctcga
     3481 tttgcccgtt ccgtttttgc cgatcaaacc gacgcgctga ccgggattga cggtagcgtt
     3541 ggctttgtcg agcaggactt tcaaaccgcg ttgcagggtg aggtttttga tttcaatcat
     3601 aacggaaaca tcgtcgggcg ggaaaagccc gtattttacc tgaaagtcag tgccgatgcc
     3661 gtctgaaacg ggaaatttac ggctgaagcc aagcccaagc cctgcgccct tccgagtgca
     3721 ggaaaaccaa tgtcctgaat gccgaatcgg tattcataca ttccacgccg attccgattc
     3781 gggaaaaatc cgccatgatt ttgggatgga taaactcctg agccgcgccc gtcccgataa
     3841 tcaatatttc cggatagtca tccgctttga catcagacaa cagattatcc ggagtcagtt
     3901 ctgacaatga ctgccatttc gagaggccgg cagaatccct atgcagcagc acaggcttat
     3961 tgaaatgtct gccggacagc cggatttcac ccgtgcggcg gtcatactct gcaaaatgtt
     4021 cgtctatcgg attttcttca aataacattt ttttatcctg ttgccgcttc atctgtatca
     4081 ggaggggtgc aaaatcagac aaattcatgt aggattagcg gatttcatct gacctctcct
     4141 gctgatttca gacggcattt gattcaaagt gcggcacaat tatatcggca gcggatattt
     4201 tcgtctttca atatttacat ttcagtcggc ttacaaggag acacaatgaa gccagtaaac
     4261 atcggtcttt taggtttggg tacggtcggc ggcggtacgg ctgccgtgtt gcgggacaac
     4321 gcggaggaaa tttcccgccg cttggggcgc gaagtgcgca tcagtgccgt atgcgacttg
     4381 agcgaagaaa aagcccgcca aatctgcccg tccgcagcct ttgtcaaaga tccgttcgaa
     4441 ctggtcgcgc gtaaagacgt cgatgtcgtc gtcgaattgt tcggcggcac gggcattgcc
     4501 aaagatgcgg tgttgaaagc cattgaaaac ggcaaacaca tcgttaccgc caacaaaaaa
     4561 ctgctcgccg aatacggcaa cgaaatcttc ccgctggcgg aagaaaaaaa tgtcatggtg
     4621 caatttgaag cggcagtagc gggcggtatc ccaatcatca aagccctgcg cgaaggtttg
     4681 gcggcaaaca ggattaaatc catcgccggc attattaacg gcaccagcaa cttcatcctc
     4741 tccgaaatgc gtgaaaaagg cagcgcgttt gccgatgtac tgaaagaagc gcaggcactg
     4801 ggctatgccg aagccgaccc gaccttcgac atcgaaggac atgacgcggg ccataaaatc
     4861 accatcatga gcgcgctggc attcggcacg ccgatgaact tttccgcctg ctacctcgaa
     4921 ggcatcagca aactcgacag ccgcgacatc aaatacgccg aagaacttgg ctaccgcatc
     4981 aaactgttgg gcatcacccg caaaaccggc aaaggcatcg agctgcgcgt ccaccctacc
     5041 ctgattcccg aaagccgcct cttggcaaac gtcaacggcg tgatgaacgc cgtgcgcgtc
     5101 aacgccgata tggttggcga aaccttatat tacggcgcgg gcgcgggcgc attgccgacc
     5161 gcttccgccg tggttgccga tatcatcgac atcgcccgcc tggttgaagc cgataccgcc
     5221 caccgcgtac cgcatctggc gttccaaccc gcgcaagtcc aagcgcaaac catcctgcct
     5281 atggacgaaa ttaccagcag ctactacctg cgcgtccaag ccaaagacga accgggcacg
     5341 ctggggcaaa tcgccgcgct gttggcacaa gaaaacgtgt ccatcgaagc actgattcaa
     5401 aaaggcgtga ttgatcagac cactgccgaa atcgtgattc tgacccacag cacggtcgaa
     5461 aaacacatca agtcggcaat cgcagccatc gaagcactgg attgcgtgga aaaaccgatt
     5521 accatgatcc gcatggaaag cctgcatgac tgagccgaaa cacgaaatgc cgacggaaga
     5581 gcaggttgcc gcgcgcaaaa aagcaaaagc caaaatccgc accatccgca tttgggcatg
     5641 ggtcattttg gcgttgctcg cttcaaccgc cctgctctcc caatgcgcga tgtccaaacc
     5701 gcaggcaaaa cagaaaattg tcgagtcttg cgtgaagaat attccgtttg ccgaaaaatg
     5761 gcaaaacgat ttacgggccc gcggtttaga ttcaaacaat acccgcctta ccgtcgacta
     5821 ctgcaaatgt atgtgggagc agcctttgga cagattgagc gagaaacaga ttagatcctt
     5881 cggcaaactc ggcgcacaag aacagcttga cctgctcggc ggcgcaaatg cctttgaagc
     5941 acgtgacaag cagtgtgttg ccgatttgaa atcagaataa tgtggaccga taaaaaagcc
     6001 gattctttaa agaatcggct tttttcataa aaaacggctt acagtgcgtc tttcaaagct
     6061 ttgccggcgc ggaatttagg cgttttggcg gcggcaatgg tcagaggctc gccggttttg
     6121 gggttgcggc cttggcgttc cgcacgttcg cccacgtaga aagtaccgaa accgaccaaa
     6181 gtaacggtgt cgccttgttt cagggcggtg gttactgcat tggtagtggc atccaaagct
     6241 ttttgtgcgg cggctttgga aatgtcggct tcttgagcaa tcgcttcgat caattcagac
     6301 ttgttcacaa tcagtccctt cctgtcttaa aaaatgatga aatgcccgaa tactcggggt
     6361 ttgtactgct tgagcaactt tcgctttata gcaattctga aattgccgtg tcaagcaaaa
     6421 aatacggaat caccctattt gacaggcttt caggacgaaa ccgcattttt acaacacatt
     6481 tcctgcgttt caatgtttgg ttgccctgct gcggggtttt ggttttgaag cggattccgc
     6541 cgccgcttcc gcaccagaag gttctgccca aggctcaggc tggctttcca aacccagagc
     6601 caatacctcg tctatccatt tgaccggatg gatggtcagg ccggttttca cgttttcagg
     6661 gatttcttcc aagtctttga ggttgtcttt tggaattaaa acgtgtttga tgccgccgcg
     6721 aagcgcagcc aacagttttt ccttcagacc gccaatcggc aggacttcgc cgcgcagggt
     6781 aatttcgccc gtcatcgcca catcggcgcg taccgggatt ttggtaaagg cagataccgc
     6841 cgccaaggtc atcgcaatac ccgcactagg gccgtctttc ggcgtcgcgc cttcgggaac
     6901 gtggatgtgg atgtcttttt tctcgtaaaa atcaggagcc aaacccactg attccgcacg
     6961 ggagcggaca accgaccacg ctgcggacac ggattccttc atcacatcgc ccaactggcc
     7021 ggtgcactga atcacgccct tacccggcaa tgctgcggct tcgacggtca gcaattcgcc
     7081 gccgacttcc gtccacgcca aaccggttac ttgtccgata cggttttcgc tttcggcaac
     7141 gccgtaatcg aagcggcgca cacccaaata gtcgtgcaga tttttctcat ttactttaac
     7201 cgccctgggc ttggcttttc cggttttttt ggtttttgag tcttcgtcca aggtaatctg
     7261 catcaccacc ttgcggcaga ttttggcaat ctcgcggtcg agcgaacgca cgcccgcctc
     7321 gcgggtgtag taacggataa tatcgcgcac cgcgctttct tcgattgcca attccccttc
     7381 ttttacgccg ttgcgcttca tttgcttcgg cacgaggtac tgcatcgcga tattgatttt
     7441 ttcgtcttcg gtatagccgg acagacggat gatttccata cggtcgagca gcggtgtcgg
     7501 gatattcagg ctgtttgaag tcgcaataaa catcacatcg ctcaaatcgt aatccacttc
     7561 ggcataatga tcggcgaact tgttgttttg ctcgggatca agcacttcga gcaacgcgct
     7621 ggcgggatcg ccccggaagt cgctacccaa tttgtcgatt tcgtcgagca ggaacaatgg
     7681 gttttttacg ccggcttttg ccatattctg caaaatctta ccgggcatgg agccgatata
     7741 ggtgcggcgg tgtccccgga tttcgctttc gtcgcgcacg ccgcccaaag ccatgcggac
     7801 atatttccgc cccgttgctt tggcgatgga ttcgcccaaa gaggttttgc ccacgcctgg
     7861 agggccgacc aggcacagaa tcgggccttt gagtttgtcc atacgttttt ggacggcgag
     7921 gtattccaaa atccgttctt tgactttttc caggccgtag tggtcggtat ccagcaccaa
     7981 tccggctttg gcgatgtctt tgctgacgcg ggattttttc ttccacggca gttcgagcag
     8041 ggtgtcgatg tagttgcgta cgacggtgga ttcggcagac atcggcggca tcattttgag
     8101 ttttttcagt tcggacaggc atttttcttc cgcttctttg ctcatccccg cctttttgat
     8161 gcctgcttcc aaggcatcca gttcgccgtt ttcgtcttct tcgcccaatt ctttgtgtat
     8221 cgctttaatc tgttcgttca gataatattc gcgctgggat ttttccattt ggcgtttgac
     8281 gcgtccgcgt atgcgttttt cggcctgcat aatgtcgagt tcggattcca gctgtgccag
     8341 caggaattcc atccgtttgc cgatttcggg aatttccaaa atctgttggc gttgcgccag
     8401 tttcaactgc aaatgcgctg cgaccgtatc ggttagccgg ctgttttcgg caatgccgtt
     8461 gatgctgccg ataatttcgg cggggatttt tttattgagt ttggcgtatt gttcaaactg
     8521 cgccaacagg gtgcggcgca cggcttcgag gtcggtattg ccgcccgtgt cttcttccac
     8581 gaccgcctct atatgggaaa cgaacagacc gcccgtgtct tcgatggtca gaacacgtcc
     8641 gcgatacaga ccttcgacca atacttttac cgtgccgtcc ggcagtttca atacctgtaa
     8701 gacttgtgcg acggtaccgg tctgatacag gtcggcggca accggttctt ctagcgccgc
     8761 atcggtttgt gccaatagga aaaccggttc ctcgcgggta atggcgtttt ccagtgcggc
     8821 gatggatttc ggcctgccga caaacagcgg cagaaccata tgcgggtaaa cgacgacatc
     8881 ccgcaaagga agggttgcca aggcggcata ttcctcaaaa tgcttttctt tttgtgtcat
     8941 aggtactctc ttgtgtctga cagattgccg attttcgcgt acattggggt tgaaggtatt
     9001 atttcaagca tatgtggttt atttatggag tttgatgcga tgccgtctga aacattccgg
     9061 cttcagactg catgggcttg gaaagacaag gcgggaacaa aaaactgttc tgtgttgccg
     9121 ctccttgctg taccatccgt atggtttacg gttctgccgc cctattttca aaacatgacg
     9181 caggtatccc atgtcttctt ttatttttcc cgataacggg gccgaatggc gcaacgaaag
     9241 tgtgcaaaag ccgcccaaag gttggcatta tgtccgcgaa agcggtgcag acggcatttt
     9301 gaaggctgcc tatcaaaata ttgcaactgt ctggcagggc gatttccaca atgccaaaca
     9361 ggtgctttct gcaatgaaga agagggctcg caaacctgcc gccgtccgtt ccgatgcgga
     9421 tatcgccgcc cttttccatg cccaccgtat gaagcaggcg cagcagagcc gtattctgaa
     9481 tatgcttgcc gttgaaatcc gccccggttt tgtgttggac aacaaacgcg cgcccgatat
     9541 acgcgccgcc ctgctcgacg tgtacggaga ggcggacggc aaaccgtttt tcctgccgct
     9601 caatctgctg ctggggttta tgggtgcgca cgagtggcat aagaaagggg ttgccgttcc
     9661 gcagctgggc ggcagcatac acgttccttt cggcgtattc tcgccgttgc gcggcgaata
     9721 cctcgacctg ctcgcccatg cgccgtcaac gggttttcag acggcattcg atatcgggac
     9781 gggctcgggc gtgcttgccg ccattttggc gaaacagggc attccttccg tcatcggcac
     9841 ggataccaat ccgaaagccg tcgcctgcgc ccgtgccaat attgcccgtt tgggctttga
     9901 aaaacaggtt gagatacggg aaaccgatct gtttcccgaa gggtttgccg atctgattgt
     9961 ctgcaatcca ccctggcttc ccgccaagcc gacttccgcc gtcgaatccg cgttatacga
    10021 ccccgaatct gcgatgctgg ctgcgttttt gcgggatgcg ccgaaacatc tgaatcccga
    10081 cggagaaatc cgcctgatta tttccgacct tgcagagcat ttggggctgc gccctgccga
    10141 ttttttggaa aaggcattta tccgggcggg tttgcgtgtt gccgatatga tgaaaaccaa
    10201 gccgaagcac aaaaaagccg cgaatccgag cgatccgctt gcttttgcgc gaacccggga
    10261 aaccactttc ctataccgtt tgaaaaaggc ataaggggcg gcggcgcggc attcgggcgg
    10321 attattcttg tgaaaatacc cgctcgagca tactgcccaa tgccgtctga cgcgttttga
    10381 cagtggcggc agcttgccgg aaggtttctt ctccgccgaa gaatacgaac ttctccctcg
    10441 cgcgggtaat ggcggtatat aacagctcct tactcaatcc ggacaatgca tcgtcccctt
    10501 cgtccgaagg tgcggcggaa ggcggcagca gccagacttc ccgatattcc gaaccttggc
    10561 ttttgtggac ggtcatggcg aatgcgggtt cgaaatcggg caggcagctt accgctacct
    10621 ttttaaatcc gtccgcatcg gcaaaatagg cggcaaggct gccctgccgt ccgacatctt
    10681 ccataatcag tccgatgtcg ccgttgaaca gttcaagcgc gtagtcgttc tgcctgatca
    10741 taatcggctc tccggcgaaa taggccagat gttccggtat gttcattttg cggcgtacat
    10801 ggctgcaata ggctttgttg aagtcttccg catcctgccg ccaagctgcc aggaccacga
    10861 tatccgagat gcctgcgtat gcggcttcga tattgccgtc ttttaccgcc tgccaatagg
    10921 ctttgtgcgc ccggtacaac ctttcgactc gagcgttcgg actgcattcc gaatgttcca
    10981 gttcatccgg aaaccggtca aacaatgccc acgccccctc atcgcccgat acggcggcac
    11041 gggcaaggca gccgatgccg ctgttgtcgc cgaaacggtg gctgaacgac agatgggcgg
    11101 tgttttgcgc caacacgggc ggatttgcgc tgacgctgaa accgtgttcc ggaaggaagc
    11161 cggccagcct ttggtgcgct tctccgtcca aaacggtttt ttgcgacaaa acggacagca
    11221 ccgccccgat tccgacggac gggagctggt tttcatcccc cagcagaatc acgcgcgcgc
    11281 cggttttgac cgcttttaaa agttgcagca tcaatgccgt atccagcata gaggcttcat
    11341 cgataaccaa tacgtcaaac ggcagcgggt aaacagggtc gaacgccgcc tgcattttgg
    11401 gcgggcgcag cttcagcagt cggtggacgg tttgcccttc cagtttgagc aaatggcggc
    11461 ggacggcctc cggcgcgtca aaaccgttga ttgcacggtg cagcgcgcgc gccatatgtg
    11521 ccgccgcttt gcccgtcggt gcggcaagcg cgatgttggg aagattttcg tcttcaccgc
    11581 aaatcagcgc cagcagtttg gcaaccgtcg tcgttttacc cgtcccgggt ccgccggtaa
    11641 tcaccataaa aggctgcaac agtgccaagg cggcggcatc gcgctgccct tcgctgcccg
    11701 tgccttgaaa ccattttgcg aggttttgcc gcgcgcctgc cgcgtcgggg gcggacgtgt
    11761 cggctgccgc caagcgtttt atctcggcag ccaaatcgta ttccaactgc cacatcctgc
    11821 ccaaaaacag ccttctgcct tccaaaatca aaggcgcggc ggatgttccg accacgggtg
    11881 cgagtgccga cagcgcgtca gcctcgccac cgctcaaacg gataaacgaa tgaccgtttt
    11941 gcaatgcctg aaacaggcgt tcggtgcagt ttgcaagcac ttcgctgcct gaacccgcat
    12001 agcgtgccaa aaaccagatt gccgcccttg ccgccgcttg ggcaaattca tctgtctgcc
    12061 gttccattgt attccttgtc gaaatgccgt ctgaaggcgt ggggcttcag acggcgcggt
    12121 gttgtttcgg ctgtttaggc gtttgcgccc tgtttggggt gcaggctgcc gtctttcatg
    12181 accatcacgc gctcgaagcg gccggcgagt tcgtcgtcgt gcgttacgac caccagcccc
    12241 gttcccaatt ccgttttcag ttccagcatc atatccaaaa cattcctggc gttcgcacgg
    12301 tcgaggttgc cggtcggttc gtcggcaagc aggcattttg gttgcgtaac caaggcgcgc
    12361 gcaatggcgg cacgctggcg ttcgccgccg gacagttcgc ccgcgcggtg cgtcgaacgg
    12421 tgtttcagtc cgaccttttc cagcatcgcc attgccgttt ctgccgcctc ttcccgtttt
    12481 ttcttgccga tcagaagcgg catcatcacg ttttccagtg ccgaaaattc aggcagaaga
    12541 tggtggaact ggtacacgaa accgagatgg cggttgcgca ggatgcccag ttgtcgctgg
    12601 cttaatgtac gcaaatcctc gcccatcagc agcaccctgc cttcagacgg catatccagc
    12661 ccgcccagaa tatgcagcag cgtcgatttt ccgctgcccg aagaaccgat gatgccggtg
    12721 ctttcccccg cgcggatttc caaatccaag ccgtgcagca cccgaacgtc caaaccgccg
    12781 tcgcggtagc gtttgcccac gccttcgcat ttcaaaatca actcactcat aacgcaaagc
    12841 ctccgccggt tgggtttttg aggcacgcca gctcgggtag agcgtggcaa cgaaagacag
    12901 tcccaaggaa atgcaggcaa tcagggcaac gtcgcccata tcgacatcgc tgggcaggta
    12961 gtcgataaaa taaacctgcg aattgatgag gtggacaccg agcaggtttt caaaaaatgc
    13021 cacgactctg ccgacgttcc aacccaaaag cacgccgcag accacacccg ccagcgtgcc
    13081 gaaaaagcct gaaaacgcgc cctgcaccat aaaaatcttc atcacgccgc caggggaaag
    13141 gcccaaagtc cgtaaaatcg caatgtccgc ctgcttttcc gtaaccgcca tcaccaagga
    13201 agagacaagg ttgaacgccg ccacggcgat aatcagcgtc aggatgatga acatcatccg
    13261 tttttccaac tcgaccgcct caaaatagct tcgattgctg aacgtccaat cgcgcaccca
    13321 aaccgtatcc ctttgcgcct ccggaatcag tgttgccgtc aaggcggggg cgttttgcgg
    13381 gtcggcgagt ttcagccgca gccccgcaac ttccttatcc aagcggtaca gtacgcgcgc
    13441 gtcttggata tgcgtcatcg ccaatgagtt gtccacttcg taaacgcccg tcttaaccag
    13501 accgaccacg gtaaactgct tcaggcgcgg cacgactccg gcaggggtaa cgttgccctc
    13561 cggcgtgatg acggtaactt tattgccgac ttccgccccc aaagcctccg ccaagccgac
    13621 accgaggata atgtcaaact cgcccggaat caggtcttca aatttgcctg ccggcatttt
    13681 gtcgccatat tccaccactt tgcgttcttc agacggcaaa atgccgcgca tctgcacgcc
    13741 cctgatttcg cccgcgtttg ccagcagagc ctgattggaa acatagggcg cggcagccaa
    13801 aatacctttg cggttttccg taaaccgaag caggttgcgc caatccgtat ccgtattgtc
    13861 gatatagccg atttcggcgt gcggcgcgac attcaggagc tgcccgcgta tttctttctg
    13921 aaagccgttc ataaccgaca agacgacaat cagcgcggtt acgcccaagg cgattccggc
    13981 aatcgaaacc atcgtgataa acgacataaa gccgttgcgc tttttcgccc tgagatacct
    14041 caagcctatc caagcctcta gagaaaacat aacgctacct taaaaatgtc tgcaaacgtg
    14101 ccgccccgga cggcggtttg ggggcggcgg caaaagttta ttgtaccgta aaacccaggc
    14161 agcgtccgaa cgcccgagtg cgggggacgg ggcggcagag cgggcggaaa acttgcacaa
    14221 cgcgtcaaac tgccctatcc tttccttcag aaaaaccgtt tcttgaggaa aacaatgaat
    14281 atccgaactg cttttgcttt gtgcgccatc gccttatccg ccgctgcggc tgcctacgcc
    14341 aaagaaatca aaatcgatgc caacaacacg ccttattccg aagccgacgc gcaaaagctg
    14401 gcggcaacgg cagtcggtat gggtgttaag gaacctatca gcctgaacgg cggcagcggc
    14461 agcattaccg tgtccggcag cagcgcgacg cagtgcgtgt tcaaagtcgg caacggaggc
    14521 gcgttgcaga ttcaagggct aaactgcaag taaaccgccc ggaaaaatgc cgtctgaagc
    14581 cttcagacgg cattttgcat tggcggcgtt atgccccgcc ttctttaatc aggcggcgtt
    14641 cgtacaccgc ctgcgccagc gttcccgcat cgacatattc caattcgccg cccaagggaa
    14701 tgccctgcga cagcctgctg actttgtaag gcaggttttt aaaaaactcg gacaggacat
    14761 acgccgtcgc attgccttct gcggtaaaag cggttgcaat aatgatttct tcgacttccc
    14821 cgccgcccag ccgttgcgcc agcctgtcca atgcgatggc ggatacgtcc attcccaatg
    14881 ccgtattgat ttgccccatc aggacgaaat acagcccgtc gtggcagttt gccgcttcca
    14941 tattcgacac gtcggcaggc atatgcacca ccatcagccg ccgcccgtcg cgtgtttcat
    15001 cggcacaaat atcgcacaat ccgccttcgc aaaacgtgtt gcacatcgcg caatggtaaa
    15061 cctgcttcaa tgccgtctgc aaggcatcca ccagtttttc agcctctttg cgcttgtgtt
    15121 ggagcaaatg atacgctatc cgctgtgccg atttcggccc gacgttgggt aaaaccttca
    15181 gcgcgtcgat caatccttgg aaggcatctt gttttttgtg gctcatcata ttccgccgta
    15241 tgggaaaacg gccggaatat tccgaccgtt attttgtcaa caaaagtgtc aattactgac
    15301 cgtcgccgtt gtcgaccgat tgcgctcctt tggtctgttt gattttgccg ttgaaataac
    15361 gtatcaacaa gtcgaaagta ttggcagact gctgttgcgc caaagcctgt tttgcaagcg
    15421 gaagctgtgc ggcgatatca tccggcgggg ttacagcctg tacttcgaca atcacgggtg
    15481 ccggcagacc gatcagcctg acgtaggcgg gtttgccgtt tgccggtttt gctttcagca
    15541 gttccgcata agcctcgggc ggcatggact gccttgcctg ctgtgcgccc aaaacggaca
    15601 cttccgacca tttcacgtca acagccttgc cgccgttcag ttgggtaagc acgtcttttg
    15661 ccttgttttc ggcaagtttg gcggcttcgg tacggatata agcctgacgt accgcgtctt
    15721 tggcttcggc aaacggcagg gttttctctt cgcggacttc tttggcgcgg acgacccacg
    15781 cggtttcgct gttgatggtc agcacttctg aattgtgttt tttcttcaat acgtcgtcgc
    15841 tgaaaacggc attgatcagg ttttcgggca taccggacat ttgcgcatcc tgcctgctca
    15901 gccaagtttc ttgggtttcg actttcaaac cgctgttttt ggcggcttcg gcaagcgagg
    15961 aaggatggtt gaacgcgtca tcgcccagct tttcttttgc cttattgaag tcggcaaccg
    16021 cctttttcat tttcaattcg ttttctacgg cggctttttc ctgctcgaaa gaaggtttgg
    16081 cttcatttgc cggcaaacgc gccacgcgct cttcaaacgc atttttcact tctgtttcgc
    16141 tgacggtctg tttgtctgca aagtctttca gattcaaggc gacatattcc aatttgaccg
    16201 ctttgggaag caggtagtct tttttgtttg cgttataaaa cttctgcaaa tcggcttcag
    16261 acactttgac ttgggcgatg aattcgtcgg ggttgaaagt gtgcgaacgg atggtgcggt
    16321 tgacctgcgt cagcctgatc agctgttccg cctgcgcgtc gccgaccaat acgccgtttt
    16381 ggacgaggtt taccaaattc tgcaaggcaa actgatcgcg gatttcttcg acaaactggt
    16441 cttcagacat atgacgttgg gaaaggtagc ggtttaaaag cgcgtggtcg aatttgccgt
    16501 ttgcgtcgtg gaaattggga tcgtccacga taatctgctt gatttgttcg gaagaaaccg
    16561 aaatgcccat cagcttcgcg ccctgtttca ggtaggcgcg ttgcagcagg gattggaaca
    16621 ccgcgtcgcg cgaagggctg ccgccgtccg cctgttcatt ttgcatagcg ttgttgatgg
    16681 agtggtcgct gattttttcg tcgcccactt ggacgatgta gtcggcaccc ggatgggata
    16741 ccgtgctgac cccgaagccg acgaaggtta atgcaatcag ccccaaaagg acttgggcgg
    16801 gcgttctgta tttttcgatg gaatggaaca tattttcaga ttggaaatgg gattgggaaa
    16861 ttcaagtcgg gtattgtaac ggtttttatc cctgtctgca cagggcttgc cgattgaaga
    16921 tgccgtcgta ggtttcttcg ctgaagccga cataaaccct gccgccgcac tccaatacgg
    16981 gacgcttgat caggctcggc atttcggaca tcagtttgac ggcctccgcc gtcgaggaca
    17041 gcactttttg ctgtgttccg gcatcaagtt tgcgccagct cgtcccgcgt ttgttgagca
    17101 gggttgccaa aggcacttgt tccagccacg agcagatttc cgcttcagac ggcctctgtt
    17161 ttttaaaatc ccgaaattca aactccaagc cgtatccggc aagcctgttt ttggcttttt
    17221 tgactgtgtc gcaatttgga atgccgtgaa ggattatcat ttggaaacct tttgtctgaa
    17281 ataataaaac ggatatttta ctataagtgt ctgaaaattt gcccgtctgt ttcagacagc
    17341 ggggcggtta tgttacaatc cgaaaattcg aaaaatttaa tctcttgttc aataaaggct
    17401 ttaccaatca tgatttctac caacggcatc accatgcagt tcggcgcgaa gccgctgttt
    17461 gaaaacgtat ccgtaaaatt cggagaaggc aaccgttacg gcctgatcgg cgcgaacggt
    17521 tcaggcaaat ccaccttcat gaaaatcctc ggcggcgatt tggaacagac ggccggcgaa
    17581 gtggcgattg aaaacggcgt gcgtttgggt aaattgcgcc aagaccagtt tgcctacgaa
    17641 gacatgcgcg tgctggacgt ggtgatgatg gggcataccg aaatgtgggc ggcgatgacc
    17701 gaacgtgatg cgatttacgc caatcccgaa gccaccgaag acgactacat gaaagccgcc
    17761 gaactggaag ccaagttcgc cgaatacgac ggctacaccg ccgaagcgcg tgccgccgaa
    17821 ctgttgagcg gcgtgggcat ttccgaagat ttgcacaatg cgaaaatggc ggaagtcgcc
    17881 ccgggcttca aactgcgcgt attgctggcg caggcgctgt tctccaagcc ggatgtattg
    17941 ctcttggacg aaccgaccaa taacttggac attaatacca tccgctggtt ggaaggcgtg
    18001 ttgaaccaat acgactccac gatgattatc atcagccacg accgccactt tttgaacgaa
    18061 gtctgcacac acatggcgga tttggactac aacaccatca ccatctatcc gggcaactac
    18121 gacgactaca tgctcgcttc tgcccaatcg cgtgagcgcg ccctgaaaga caatgccaag
    18181 gcgaaagaaa aattgcagga attgcaagaa tttgtggcgc ggttcagtgc caacaaatcc
    18241 aaagcccgtc aggcaaccag tcgtctgaag caggcagaca aaatcaaatc ggaaatggtc
    18301 gaagtcaaac cctctacccg tcaaaacccg tatatccgtt ttgaagccga tgaaaaagcc
    18361 aagctgcacc gtcaggctgt ggaagttgaa aaactggcga aacgctttga aacccagttg
    18421 tttaaaaacc tgaacttcat ccttgaagcg ggacaacgcc tcgccatcat cggcccgaac
    18481 ggcgcgggca aatccaccct gctgaaactc ttggccggcg cgtacaaccc cgaatattca
    18541 gacggtctgc tgccggacga aggcagcatc aaatgggcgg aaaaagccag cgtcggctac
    18601 tatccgcaag atcacgaaaa cgacttcgat gtcgatatgg atttgagcga atggatgcgc
    18661 caatgggggc aggacggcga cgacgaacaa gtcatccgcg gcactttggg gcgtttgctc
    18721 ttcggcagta acgatgtcgt gaaaaaagtg aaggttctct ccggcggcga aaaaggccgt
    18781 atgctttacg gcaaactgtt gctgttgaaa cccaatgtct tagtcatgga cgaaccgacc
    18841 aaccacatgg atatggaaag catcgaatcc ttgaacatgg cgctggaaaa atacaacggc
    18901 acgctgattt tcgtctccca cgaccgtcag ttcgtttctt ccctggcaac ccaaatcatc
    18961 gaactggacg gcaaaggcgg atatgaacac tacttgggcg attacgaaag ttacttggag
    19021 aaaaaaggcg tagcataacc gccggttgga acaatgccgt ctgaagccgc ttcagacggc
    19081 attgttgata actttaaaat aggaagcata tgcagactta cctcgtcggc ggcgccgtcc
    19141 gcgattatct tttgggcttg cccgtcaaag accgcgattg ggtggtcgtc ggcgcagacg
    19201 cacaaaccat gctggcgcaa ggcttccagc cggtcggcaa agattttccc gtgtttctcc
    19261 atcccgaaac gcacgaagaa tacaccctcg cccgcaccga gcgcaaaacc gccaaaggtt
    19321 acgtcggttt cagtttccac gccgacaaag acgttacgct ggagcaggac ttgatgcgcc
    19381 gcgacctgac catcaacgcg atggcgcaag atgcggacgg caagattatc gaccctttcg
    19441 gcggacaacg ggatttggcg gcaggcattt tgcgccacgt ttccccggcc ttcgccgaag
    19501 accccgtccg catcctgcgt actgcccgct ttgccgcgcg ttacaggttt gaaatcgccg
    19561 aagaaaccat aaagctgatg cggcagatgg tggaaaacgg cgaagcggac gcattggttg
    19621 ccgaacgcgt ctggcaggag tttgcgaaag gtttgatgga aaaaaatccg cgcaaaatga
    19681 ttgaagtgtt gcgcgaatgc ggcgcgctca aagtcttgct gcccgaagtc aatgccctct
    19741 tcggcgtgcc gcaacgcgcc gactaccatc ccgaaatcga cagcggcatc cataccctga
    19801 tgacgctgca acgcgccgcc gatatgggtt tgagcctgcc cgaacgctat gccgccctgc
    19861 tgcacgactt gggcaaagcc aaaacaccgt ccgacatcct gccgcgccac cacggacacg
    19921 acctcgccgg tgtcgaaccc gtgcgcgaag tcaatcagcg gctgcgtgcg ccgaaacatt
    19981 gcgccgagct tgccgaattg gtttgccgtt ggcacattat tttccaccaa gtcggacagc
    20041 ttaaaagcca aaccattctg aacgtattga aaaaaaccga tgccttccgc cgtcccgaac
    20101 gctttcagac ggcattgaac gtctgcattg ccgacacgca aggccgtctg aaccgcgaac
    20161 acacgcccta cccgcaacgc gcacactggt tcgccctgct cgaagccgcc aatcaggcgg
    20221 attcgggcaa aatcgccgcc gaatgccgcg cgcagggaaa agcgcacctt atcgccgaac
    20281 aaatcgaccg ggcgcggctg gcacaaatcg ccccattgca aaaaacgttt cgagcggcgc
    20341 aagacaaaac agaaaaacat taaaacgtcc aatgcagcca
//
LOCUS       DAWFAD010000022        19820 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWFAD010000022 DAWFAD010000000
VERSION     DAWFAD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19820
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.022"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(232..307)
                     /locus_tag="WGO49_000590"
     tRNA            complement(232..307)
                     /locus_tag="WGO49_000590"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(272..274),aa:Asn,seq:gtt)
     gene            complement(400..759)
                     /locus_tag="WGO49_000591"
     CDS             complement(400..759)
                     /locus_tag="WGO49_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ0305733.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(782..1426)
                     /locus_tag="WGO49_000592"
     CDS             complement(782..1426)
                     /locus_tag="WGO49_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ0305734.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(1428..1964)
                     /locus_tag="WGO49_000593"
     CDS             complement(1428..1964)
                     /locus_tag="WGO49_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ0305735.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1976..3073)
                     /locus_tag="WGO49_000594"
     CDS             complement(1976..3073)
                     /locus_tag="WGO49_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0305736.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLASVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3076..4512)
                     /locus_tag="WGO49_000595"
     CDS             complement(3076..4512)
                     /locus_tag="WGO49_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ0305737.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQHADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4732..5514)
                     /gene="bioC"
                     /locus_tag="WGO49_000596"
     CDS             complement(4732..5514)
                     /gene="bioC"
                     /locus_tag="WGO49_000596"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ0305738.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(5502..6149)
                     /locus_tag="WGO49_000597"
     CDS             complement(5502..6149)
                     /locus_tag="WGO49_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ0305739.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKVSFEDYQQFPARPFDEIHQELTALFEMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6159..7301)
                     /gene="bioF"
                     /locus_tag="WGO49_000598"
     CDS             complement(6159..7301)
                     /gene="bioF"
                     /locus_tag="WGO49_000598"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ0305740.1"
                     /translation="MNVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENRKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEQLVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAVCL"
     gene            7524..8600
                     /locus_tag="WGO49_000599"
     CDS             7524..8600
                     /locus_tag="WGO49_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ0305741.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8807..10273)
                     /locus_tag="WGO49_000600"
     CDS             complement(8807..10273)
                     /locus_tag="WGO49_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ0305742.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10471..11394)
                     /gene="speB"
                     /locus_tag="WGO49_000601"
     CDS             complement(10471..11394)
                     /gene="speB"
                     /locus_tag="WGO49_000601"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ0305743.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11493..13385)
                     /gene="speA"
                     /locus_tag="WGO49_000602"
     CDS             complement(11493..13385)
                     /gene="speA"
                     /locus_tag="WGO49_000602"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ0305744.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYASTVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13552..13836)
                     /locus_tag="WGO49_000603"
     CDS             complement(13552..13836)
                     /locus_tag="WGO49_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0305745.1"
                     /translation="MKKLLLAAVVSLSAATAFASDSAERQIYSDPNFEQNRVKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13987..15795)
                     /gene="aspS"
                     /locus_tag="WGO49_000604"
     CDS             complement(13987..15795)
                     /gene="aspS"
                     /locus_tag="WGO49_000604"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ0305746.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYAEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAE
                     NGDIIFFGADKAKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(15853..16554)
                     /locus_tag="WGO49_000605"
     CDS             complement(15853..16554)
                     /locus_tag="WGO49_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ0305747.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16697..17824)
                     /locus_tag="WGO49_000606"
                     /pseudo
     CDS             complement(16697..17824)
                     /locus_tag="WGO49_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236077.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
     gene            complement(18005..18268)
                     /gene="rpsT"
                     /locus_tag="WGO49_000607"
     CDS             complement(18005..18268)
                     /gene="rpsT"
                     /locus_tag="WGO49_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ0305748.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18571..19713
                     /locus_tag="WGO49_000608"
     CDS             18571..19713
                     /locus_tag="WGO49_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0305749.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5108 a         5107 c         4596 g         5009 t
ORIGIN      
        1 agaatcgttc tctttgagct aaggcgagcc aatgccgtac tggtttttgt taatccacta
       61 taaaatcgag tttatcctag ttgtccagga cagcccctat tttttcataa ccatcacgaa
      121 aggaattttg caaagatttt attccatctc aaaacaatca tctcaaaaat gcgtttctga
      181 ccgccggtaa aacaaaaccc tctaagaaaa tacttagagg gttttgaaat ttggctcccc
      241 gacctgggct cgaaccaggg acctgcggat taacagtccg tcgctctacc gactgagcta
      301 tcggggaata agcgcgaata ataaaacaga aataccgaac gtgtcaatat aaattgacaa
      361 tcgattgcac tttatctgca agcccgcaat ctttatatgt tatatggctt ccgcaatatg
      421 ccttgccgca cgtttggcat ccaataggat taaaccaagt ctgccggttt cttttgccac
      481 cagcaccaac acggcatctt tacccgcctg acttaaaagg atatagcctg attttccttt
      541 aatcatcact tgttccaatt ccccgcaggc gagttcctgc accgagcgac tccccaaagc
      601 aagcaaagtg gcagaaatcg cccctaccct gtccgaattc aaatgtgaag gaagcattgt
      661 cgccatcggc aatccgtcgg ttgagataac ggcagacgcg ataatatccg tagatgtatt
      721 gtttaaatct tcaaggattg aaatcaataa ttgctgcata acccctcctc tctcccaagt
      781 tttacacgcg gttgctgtaa cggcggtata aaatccttac caaagtaaca aatgcctctt
      841 tgcccaaatc gggaatgccg ccgataacca aaataaattt ggttgaaccg atatacaatg
      901 ggaaaaatgt caattcgctc tgaccggaag gatcgcaaac gccccaagcg ttattgttga
      961 tatacaggtt gttcttaatc agcagccggt atttcttttc catctgtgcg acttctgccg
     1021 ccaacaaccc caactcttcc gccgcctcat gatggaaatt ggcgttggca agatacagac
     1081 cgttccgatc gaccaataac gccttaccgc tgccggacaa ttgttccatc agcaacggca
     1141 attgctcgtc cgacaaattg atgccgtctg aatgaccgtt ttcatcgcca tagaggaatt
     1201 cgagtttttg caaacggtac aacaggttca aagcggtatc gatgtcggcg gtgtccgccc
     1261 aggtaagcag cttctcactg ctgaccattt cgtccgcatc cgctttcaac aggctgtgca
     1321 acaaagtttt accggcactg ggggcatcgc tggatacggc ataaaatgca ccggcaggag
     1381 tcaggcgggg atataaattt gcttgtagtg aaagtgttga ttccatatta aacctccagt
     1441 cccggatcaa tagaaaataa cattgcgcta accaattgtt ttacgtcatc ttccttacgg
     1501 gcatcaattt caaaaaccgg aacattaaga ttatgttttg caagatattt gtgatacacg
     1561 tcgataccgg gctgagagcg tatatccatc ttggtaatac cgacaacgac gggtgccttc
     1621 tccagcagcc ctcgaaacga atgtaaaaag aattccaaat ctttcaacgg attggttcgg
     1681 gcattatcta aaagcaagac caaacccata ctgccttggc ttaagatttc ccacataaag
     1741 ttgaaccgtt cctgaccggg cgtaccatat aaatggactt tggtatcctc atccaagctg
     1801 atggccccgt agtccatcgc cactgtcgta ttccttttcc tatccaaagt catatcggat
     1861 gcggaagcat cggtctgaac gagtgcttcg tccgaaatag ccgcaatggc agtggttttc
     1921 cctacgccga caggtcctgt gaaaataatt ttattttctc tcatctctcc gcctcttagc
     1981 tgcccaacag ttttttcatc agcctttgca gaaggccgcg cgactgggat tgggatggtg
     2041 ttgtgatttt ttccgctttt ttcatcattt cactatcaga ggcagaatcg gctccgatat
     2101 ttattttatc cgccatatcg gagtatgcct gttgtgaaac cgtttttaaa tctaccgaca
     2161 aaaacccggt tgtataggtt gccgcaagat aattcagaat atcattgagg tttaaaggca
     2221 tcactttata caacacgtta aggttgacgg atgccttggt cagaaatgcc gacaagcgta
     2281 tcgaccccgg cacacttgcc aaccgggtca ggtttggcca agatttcaac gtaaacggag
     2341 tatcgggaga aatcggataa atcaacctgc cctgcgccgt ccaaatggaa aactgccaca
     2401 tacaggacat aatgcctact ttagcctttt cgcgccattg cgggttatcg ggaacagtct
     2461 tgcagctgac ctgcaaattt tcgtctttgc acaattcttc gagtttttgc acactttctg
     2521 tcagcaaaac ccgttgtatc gaggggaaaa caataaggac cggcttattt ccatgcaaga
     2581 tagcgatgtc ctgcctgttc ttttccgcaa accgcaacgc ccccaataat cctttattcg
     2641 ggttaaactg gcgtatcgta accgtacgct gcacattccc gttatttttc gccgaccggt
     2701 ctgcgggtgc aataaacgat ttcccataaa cattctcgcc ctgcaacaat ttgcggagca
     2761 taggaaacaa tgtttcaaac cgaatcggtt tgggcaggta gggaacttca gaatcgggaa
     2821 ctttctccga acagacggcg acgggtatat ccttataacg ctcggcaagc tctttccaaa
     2881 gttcaaaacc gccctcggca tcggtatccg ccaaaaccaa atcgggcacg gcactgccgt
     2941 ctgaagggga tactgtttca taacgggtgg tattgtgcat tttgaatgcc attttgaaaa
     3001 cggattcctg ctgcgccgtc atccccgcca acattacgcg tactgtttta attttcggca
     3061 gttgaacttc catttttatt ttccgtaccg ttttattttt ttaatatttt gattcatacg
     3121 ctgcagcagc cggctcatca gcataacgac ctcttcagga agcctgtccg cacgttccct
     3181 caataccctt aaaaactgcc ccaacctatc ccaatcttca gtacgttcat aaatatcgat
     3241 caacgtaata taaagctggg actcgtcggg atatttcaat accgcctgct ccaacacatc
     3301 cattgccgct tcaatctgac catacatcag caacgactct acttctttaa ccgcatcgtc
     3361 tgccggagac gaaccggtgt tgatcaacga agaatcttga agcaccaaat cccgatgttg
     3421 cgatttgaat ttctgtatat ttttcggcag atacccgtgc cccataccga tgtctttgat
     3481 ttgccggtcg ttcggccctt tttccaaatc atcgaaaact tcatggtaac ccaagctgta
     3541 cccccacccc agcatccgct ctttaacctg cctgccgtag ttgcccaacg tttggtaaag
     3601 tttccacaaa tgtccggcaa aacggtctat gtccgcgtgt tggtaatcga gtttcaacgc
     3661 atcgataatc aggtttgcag gtttttcgga agtttggatg gcacggtcgt attgtttcga
     3721 tgccgtttca tagctgactt tgtctttaag gattttcgca ccttgatcgg cacggaccaa
     3781 accggcaatc gcaccgattt cctcttgact gattccggac acgtcttttt tgccccgcac
     3841 aatcgggatg cgcttgattt cttcggcttc ataagcctta ccgccggcat ccggcggggg
     3901 cgatgcggaa gctgccgtcg cagaaccgcc ttccgcacgt ttttcaatct cctgagtacc
     3961 ccatcccaaa ccttcttccg ccaagacgcg gatacgcaaa tggtttgaat cgcgctgtaa
     4021 tgcctgttcg atatattttg ccaaaagttc ggaaggaatc agtttgccgt atttttgcag
     4081 attgtctgcc aaaacatcga catcccccac ttcgagattg atatcgagca gctcgcggat
     4141 aaggttttca ggtttcgctt caccatccgg aatgccgtcc agataggcag ccaaagattc
     4201 ggcagccttg ccctgataac cgaattgctt ataaacctga tactccgtaa gcggatcgac
     4261 ttcttgcgcg gatacggcgg cggatgcggt ctcggcactt tcgttccaag accagtcggg
     4321 ttgcgtgccg tctgaaaccg attgtccaat ctgatcgacc cagtccgaat cattgcttcc
     4381 gggtgtctgc ccctgcgtat tgccgacacc ttgggatttg cgcttcggtg ttttcttacc
     4441 ctgcttcgca cgcataacca aaagcatcag caataccgcc agtgccaaac cgataattaa
     4501 tgagttttcc aaaggatact cccgataccg taacgagcgg atagctgacc gcctgcaacg
     4561 gccattgtcc gcttttatta ttaacgattt actgaaatag acttgtaagt tttaaatcat
     4621 accataattt aacgtttaac aatatgcctt ctgcacaagc ctcgccatat taccttttac
     4681 ccacatcagt atcaataccc gatataaaaa taactttgcc cataagctgc cttattgcct
     4741 gcccgccgca gagtagcgcg cgataaaaaa taacggatga taagtcaggt gcactttgcc
     4801 cgacggcata ctgaacgccg acaaataatc gccaataaat ccattgagat tttttcttgt
     4861 ccaatttttt tggttcgtgg ctgttacgcc tgtatattta aggtgtttga gtacatctaa
     4921 cggcgtatca aagtctagta ttaccttaaa atcctcacac cataaaagct caaaatcttt
     4981 ggctaaccaa gtctgccatt gggataaagt cgggtaattt aagcctatat ttgtaatttg
     5041 gcggacttct tttaaattgt ctttgccaaa ggttgcaacc gccaataatc cgtttgcttt
     5101 caagcctgtt ttgcaatggg cgataaaagc gtcgggttga tgaaaccatt gcacggcaga
     5161 tgcgcttgcg attaagtcaa attgtcgttg aaaaggaaag ttttccgcat cgccgcaata
     5221 aaaatcaaag gattgcggca gtttttcagc cagttggggc tgcacatcgc acaaatcatt
     5281 aaataaccaa taattcgctg aaatctgttt ttgcagcaag gcactcaaca ttcctgagcc
     5341 gcagcccaat tccaacacgt tttccaatgg catatccggc aaataatctt gcaaatgcgc
     5401 cattaaatta atcgtcattt tttgttggat taaggcgtgc cggtcataat cgtttaatgc
     5461 tttttggaaa gcctgccgaa tgcgcgattt atttatggca gtcagtgatt ccatagtgcc
     5521 gaccaatggg tgaatcttga aaacaggtaa tgtccgccgt caatttcccg aacggtgcaa
     5581 cgcggtgtcc aatatcggtg ctgattggca ggcataaaaa ttttatcgcc tgatccgacc
     5641 aaggcatttg tccagcgaat caaatctgcg cgcctatctt gcccgatcat ctcaaaaagt
     5701 gcggtaagtt cttgatgaat ttcgtcaaac gggcgtgcgg gaaattgttg gtaatcttca
     5761 aaagatactt tatcgccaca cattctgcgt tcaaatttta aacgggtgtt ttccgtgagg
     5821 ttctccaatg tgcctttaaa aacggcgcaa gggataccga aattatcatc gcaaggcaaa
     5881 cctgtgccat tcactgccgt tgcggatttt aatcttattc cttgcaatgc cctctctgcc
     5941 gcccaaacgc ccattgacca cgccaccaaa cggatgtgcc gataggcgga aaaatcaaaa
     6001 tccaaattta aatcttgata atcgtagcaa atcaataaat cgtgattttc cggcaaaatc
     6061 aaatgattta cagcatcggg cggcgttccc caacctgcaa aatacaggat taaatgtccg
     6121 ccttgatgat tgtaaaattt tgttttcata tcatatcctt acaggcacac cgcaaactgc
     6181 cgcacttcat ccattgtcat atctgccgtc aaagacaatc gtattctgga tgtacctttt
     6241 ggtactgtcg gtggtctgat gggcaggcaa taataaccct gcctttgcag gtattccgct
     6301 ttggcaaggg tggcttcatt cccgcctaaa atatagggga cgatacaggt ttggctcggc
     6361 attatttgcg tccgatgcgc cacttcccgc cgtaaaaatg cgcttaactg ctcaagatgg
     6421 cttctttctt ttgagaattg cggcaatcgt tcaaaaataa aataagtcca agccacatta
     6481 aacggcggca atgcggttga aaaaatcaat gggcgcattt gattaatcaa acattctttc
     6541 aatacttggt tgcagacggc atacgccccc accgaggcta aggctttacc gaaagtgccg
     6601 accaataaat caatctcggc aatcaaatta tcccgttcgg caatccccaa tccgttttgc
     6661 ccataaacac cgattgcgtg ggcttcatcc acataaagat aagtattggg aaactgtttt
     6721 tttaattgga caagctgttt caaatccgcc acatcgccgt ccatactgaa aacagattcg
     6781 gtaacgataa aagtgcggtc aaattttccg acgttttttt caagcagatt tttcaaatgt
     6841 tcataatcat tatgacgata acggaaaaac gcacaccggc tcaaacggat gccgtcaatc
     6901 atactggcgt gaacaaattt atctgccaaa atcaaacttt tcgtcgtcgt caaagcaggc
     6961 aaaataccga gattggcgtg atagccgctg ttgaacaata acgcgctttc ccgttggaaa
     7021 cgttgtgcga caagctgctc aagatcggta taaataggaa agttgcccgt taataaacgc
     7081 gatgaagaac tggtaaaaga gggaaaatta ccgccgtatt gctgcaaaaa agaccggcgc
     7141 aagttttcat ctgatgccaa acccaaataa tcattagacg acatattcag cattttgcgg
     7201 ttttcccgcg taatataccg cccttgatga atcaaatccg gaatcgaacg atattggttt
     7261 tgcgcgccga gttgttcaag ctgttgttta aaaacattca ttatgatgta aatattcctg
     7321 agttaaagcc tgaaccaacc cagcatcaaa caatcaagta atgccctttt aataaaaacc
     7381 gttttgccgc cgacctttgc cacacccctg ctttcataat ccaaggcgga aatttccgcg
     7441 acatttgttt ccgttgccat tacccaatcc gtccgcactg taccgaagcg catattttcg
     7501 cacaaattgg tacggtttca ggtatgattt gccgaaaacc tatcggacac acaccatatt
     7561 ttgatatgaa taaaacaatc tgccgcactg ctgcactatt gataagcggt ttttcatacg
     7621 caaacaccgt cattcccgat gtctcccccg tcgcacaagg tcaacatgtc ttcattaaca
     7681 tccctcagca acgcctgttc ctctacaccg acggcaaact gaccaaggtt tatcccgttg
     7741 cagtaggtcg ggcgatgacg caaaccaatc taggcgaaca taaaatcggt gcgaaagcct
     7801 ataaccctgt ttggtacatc cccaaatcca tacagaaaga acgcggcgac ggcgtaaaaa
     7861 ccatagccgc cggcccggac aaccctttgg ggccggtttt tgtccgctta ggcgacccca
     7921 aactcggttt gggcatacac gggaccaatg cgccggccag cgtccccggg gttcggagtc
     7981 acggctgcgt ccgcatgaaa tcgcccgacg cgctcgagtt tgccaaaacc atcgccagcg
     8041 gctcgcccgc ctccgtcatc tatcaaatgg cgggtctcaa tgaagatgcg gatcgcaacc
     8101 tgtggcttgc cgccttccgc gacccttacg gtaagaacaa ccttgacatc gcctctctga
     8161 aaaaaagtat tgcgcaatgg gcaaaaacac agggtaaaac catcgcgccc gagaaagtcg
     8221 atgctgtact caaagaccgc accggatcgg ccgtctgcct gacctgcggc aaaaacggca
     8281 agatgaagat gccgctcaaa tcgctggcgt ggatacaggg ttcttcctca tacagccaag
     8341 ctgaagtcat tgaacaaact gaggaaacca actctgccga aatatccgag acacgcacgc
     8401 ccgaagtgcc tgatgtacac acgcccgagg cacaaccgca tttaaatacc caatccgacg
     8461 gcacgccgac tgcctatacc gaaccggctg ccgattcatc gccgcaagta gaaacacctg
     8521 atcaggctgc ttccgagccg gttgatgtat tattttcaat agatgtgata cggcagggaa
     8581 atttgcgttt aggtaattga ataaccttct gattatttaa tgcttatgtt tatcagaagt
     8641 tgataggcgg tttggttttt gtttggcttt tcctacaccg ccgcctgccg ctttctgcaa
     8701 cattcaagcg cacaaatatg ccatctgaag gctttagacg gcatatttca cgataatagt
     8761 cgcccaatca aataaacaca gattggaaat ttattttatt tttcccttac caatacccca
     8821 gaactttcca ccaaatgctg ccgataacga aaaaaatcag aaaattgact acgctcatga
     8881 taaaacccgc cttccaccat tctcccattg tggtgtagcc cgaaccgaaa atcacaggcg
     8941 aagtaccggt cgcataatga gtgagggtca tcataatgtt agatgcggcc gccatcatca
     9001 gcgcggtcgg catcgccggg gcattcagtg aaacggcagc agcgaaaaat gcgccgaaca
     9061 tagcggtaat atgtgcagta gtactggcaa acatataatg cgcatacata taagcaagca
     9121 cgaggattac gcccgcagcc gtgccgctaa cgcccaaacc gccgacactt tccgccaaca
     9181 ctccggagaa ccatttaatc agtccgagtt tatttaaaaa tgcggccatc ataatcaatg
     9241 cgccaaacca aataatcgta tcccacgcac ttttttcttt caaaacatcg tcccaagtca
     9301 atacaccgga aagcaaaagc aggcttaatc cgataaatgc ggtggcggtg gcgttgatac
     9361 taaaagcgtg attgccggta ataagggcgg gaacatctgc ccacaacagc agcaagatac
     9421 cgaaaatgac cgccataatg atttcgtctg ccgacatttt acccatctcc ctcagacggt
     9481 ctttggcaaa ttgaacggca ttgggcgttt ctttaatttc aggcggatac aaaaaatata
     9541 aaatcaaagg cataacgaaa aaggcgataa cgccgggaac agccattgcc cacgcccacg
     9601 ccccccaaga aagacggaaa ctactgccta aattttcggc aatcaagttg acgattaaag
     9661 ggttgggggc agttgcagta ataaacatag ccgacgaaat gggattggaa tgatagttga
     9721 ccaaagccaa atatttaccc atcttgcctt ctgtgccttt tgcgggattg gagccgtaac
     9781 tgccggcaat cgactgcata atcggatgta taatgccgcc gccgcgcgcg gtattggaag
     9841 gggtaacggg agccagcagc agttcggaaa gagcgagact gtaaccgatg cccagcgttt
     9901 ttcttccaaa aacggcgata aacaaatatc cgatacgcat ccccagccct gttttgagca
     9961 aaccgcgcga aatcataact gcgatggcaa tcagccaaat caacggattg gcgaacgcac
    10021 tcaacgcatc gctcatcgcc gcgcccggtt tgtcggcggt tacgccggtt actgcgacca
    10081 acccgacggc aataatcgac agcgcgccca acggcatggc cttgccgata atggcggcaa
    10141 tcacaccgac aaacatggcc agcagcgtcc aagcctgagg cttgaccccg tcgggtacgg
    10201 gcagtgccaa aaccagggcg cacaatactg cggcaatggc gaggggtatc ggtttgaaac
    10261 ccaatttcat catattgacc tccgtaaaaa agaccgtccc gaaaaatcgg aaaaataata
    10321 tttaactaat tgttttataa tatatattca gatatttcac cgtctttccg atatgcggct
    10381 ccgggcaact tttgtttcag tatttgaatt ttcattagac tgaatacgcc gtttgaacgg
    10441 cacggcgaaa acccggggga tgccggacgt tcagtccttt ttcgcacctt gaaggtaaag
    10501 catttccaag gcaattgtgg cgccggccaa agcggtaatg tcggattggt cgtaagaggg
    10561 ggcaacttct acaacatcca taccgacgat gtcgagatcc gtcagcccac gtaggatttt
    10621 taatgccctg tcgctgctca agccgccgca tacgggcgta ccggtcccgg gggcgaacga
    10681 cgggtcgagg cagtctatgt cgaaagtcag gtaaacgggc atattgccga cggtttcttt
    10741 gattttacgg acggtctctt caacactgtc ttcattaact tggggggcgg tcaacacagt
    10801 aaaaggcaat tttttactgt gttcggtacg tatgccgatt tgtacggaac gggacgggtc
    10861 gatgaggcct tccttggggg cggtatagaa catcgtaccg tggtcgtatt cgctgccgtt
    10921 gtcgtaggtg tcggtgtgcg cgtcaaaatg aatcagtgcg agtttgccga aatagcgggc
    10981 gtgggcgcgc aacaacggga gggtaatgaa atggtcgccg cccaaactca aacagcgttt
    11041 gccggaagaa agtaatttgc cggcgtgcgc ttccattttt tcgacaaaat ccctgctgtc
    11101 gccaaaagaa aaaaccaagt cgccgcaatc aataatgttc aggcgttcgc gcacatcaaa
    11161 tgtccatgga aacctgcggt gctcccaagc gaggttgacg gaggcgcgcc ggatggcttc
    11221 aggaccgaaa cgcgcgccgg aacgccctga aaccgccata tcataaggca cgccggtaat
    11281 aacccaatcg gcatgacttt catacggcat aaaattaagc ggcaggcgca aaaacccgaa
    11341 attattggaa acgagggagt tgtcggtttg tcctgccagt gtgctgtatt gcatcgtaat
    11401 gattccttgt aattggtttc aatcggtcgt gatgattggt gtttgagtaa gaaaatcggg
    11461 cttcagacga catatccgat gccttgatgc gtctattcgt cttccaaata ggtataacca
    11521 ttaagccccg cttcgagttc ttttaagaaa gacatagcct gcgaggcagg aaggtctgaa
    11581 tgttcgattt gttcgcgata gcgtttcatc agctctttcg gatcttgata aacgtattcg
    11641 agcatatcgg caacggtgtt tccttcatcg taatcgatga cggtaaattg tccgtcttcc
    11701 cctacaacaa catcggcagt ggcagtgtcg ccgaaaagat tgtgcatatt gccgagtatt
    11761 tcctgatatg ctcccaccat aaaaaagcct aaaagcggcg gctcttcttc gggataatca
    11821 ggcataggca tcgtaccggc gatgccgtct ccgtcgatgt agtggtcaat cgtaccgtct
    11881 gaatcgcagg taatgtccaa caacacggcg cggcgcgcaa tcggttcatt caaaccggta
    11941 atgggacaaa cagggaaaag ttgatctatg ccccaagcat cgggcaaaga ttggaagagt
    12001 gagaaattga catacagctt atcggcaaaa cgttcttgca attcgtcaat aatggttcgg
    12061 tgagaccggt gtttttcatt aaacaattcg ccgacttcat gacagatatt taaatacagt
    12121 tgctccgccc acgcacgttg cgccaaactc aacagcccga cattatactg attatgcaca
    12181 tcggcaagat caaactgccc ttcgtgtatc cagctgcgta aggaacgttt ttcccgcgag
    12241 gcggaaatat ccgtccaagt ttcccacata ctgtgcaaca cacgcggtgc ttcgggcgat
    12301 ggcgcatcca gccggcgcgg tttgtaacgt tcaacgccta taacattagc aaccaaaacg
    12361 gcgtgatgtg cggtaatgcc gcgcccgctc tcggtaatga ttgtcggatg cggcagcccg
    12421 tgttcgagac aagcctggct gatgccccat acgactgtgg aggcatattc gttgaggctg
    12481 taattaacgg aacaatccga ttgtgtgcgg tttccttcgt aatccacgcc aagcccgccg
    12541 cctacatcaa aacagcggat atttaccccc agtttgtgca actcaacata aaaccgagcc
    12601 gattcgtgta cacctgtggc aacatcacgg atgttcccaa gctgcgagcc taaatggaaa
    12661 tgcaaaagct gcaggcagtc cagcctgttt ttttgtttca aaatatcgac cagttgcaaa
    12721 acttgggaag ccgacaagcc gaattttgat ttttccccac ccgaagactg ccattttccc
    12781 gaaccttggg aagccagtct ggcgcgcaca cccaaacggg gcttgatgcc gagtttttcc
    12841 gcctcttcca ataccatttg tatttcggac agcttctcaa tcaccaaata aacctgatgc
    12901 cccagttttt cgcccatcaa ggcgaaacgg atatattcac ggtctttata gccgttgcag
    12961 acgattaatg tttgccgggt gccggcgtgt gccaaaaccg ccatcagttc ggctttagaa
    13021 ccagcttcca aaccatgcgg ttgtccgctt gacataagcg attcgatgac gcggcggtgt
    13081 tggttgacct tgatagggta aaccaaacaa taaccgccct tatagccgca ctcttcccgt
    13141 gccgtctgaa aggcgcggtt aatgtcgcgg aggcggtgtt cgaggatttg cggaaaacaa
    13201 aacaaaacag gcaggcgcgc ctgatgtttt tgttgcacgg cttcagtcag tttttgcagt
    13261 gaaacagttt gattgtgttg cgagggattg gggcggacga tgatttcgcc ggaatcgtca
    13321 acatcataat aacctatgcc ccaatgatta atgttgcaca cttcacggat ggtaaggata
    13381 ggcataataa acctgctccg tctgtcgtgt tgaaaaggaa tgattataac aaatcagcgt
    13441 gaaatgtcat ttttttaata agaaaagcct gcctcatacc tgatgaggaa caggcaaaat
    13501 gccgtctgaa cgcttcagac ggcattttgg ttcatctttc catcaaagga gtcagcgatc
    13561 gagctgctct ttgatgattt tcaggtcggg gtaagacaac acgatgtcgt attcgcggcc
    13621 gtctttatag gcttccactt ccagcacagg cttaccccaa tggtcgtcgg catcgacatc
    13681 gtgaacctga taaccgcgct gttccaacat tttcacagct tttacgcggt tctgttcaaa
    13741 attgggatca ctgtaaatct gacgctcggc agagtcgctg gcaaatgcgg ttgcggcact
    13801 cagggaaaca acggcagcca ataacagttt tttcattttc agtcctttct ttatcggttg
    13861 attgaataag atgtgttttt caatgccgcc attaaaacac agcaaaatta ggtttgaatt
    13921 agagggaagt caagaagcgt aaatgccgtc tgaaaaaaac agagccgtca aacggctccg
    13981 ttttccttat gcttctttgg tttcagccgc cttttggcgc aggcgcaggc tcaattcacg
    14041 caactgcttg tcgtccacgc tgttgggcgc gttggtcagc aggcattggg cacgttgtgt
    14101 tttcgggaag gcaatcacgt cgcggataga ttcggcgccg gtcatcaggg taaccaaacg
    14161 gtcgaggccg aatgcaaggc cgccgtgagg aggcgcaccg aatttcaggt tgtccaagag
    14221 gaagccgaat ttctcttgtt gctcttcagg gctgattttc agcgcggcaa acactttctc
    14281 ttgtacgtct gcacggtgga tacggataga gccgccgccg atttcccagc cgttcaatac
    14341 catgtcgtag gcacgtgcca aacaatttgc cgggtcggaa accatcaggt cttcatgacc
    14401 ttcttttggc gcagtaaacg gatggtgtac ggcaacgtag cggtcggctt cttcgtcgta
    14461 ttcaaacatt gggaagtcga caacccacaa aggtttccat tcgtctgtga aatagccgtt
    14521 ttccgcgccg tgttccaagc cgactttgat acgcaatgcg ccgatggctt cgttcacgac
    14581 tttggctttg tccgcaccga agaagatgat gtcgccgttt tccgcgccgg tacgcgcaat
    14641 gatttctttc agggcattct cagacaggaa ttttacgatt ggagattgca ggccgctgtc
    14701 ttcgccgttt gagaggttgc ccacatcgtt tactttgatg tatgccagac ctttcgcgcc
    14761 gtagatgccg acaaatttag tgtattcgtc gatttctttg cggctgaatt ttgcgccgtt
    14821 cggtacgcgc agggcgacta cacggccgcc tttcatatct gccgcgccac ggaagacttt
    14881 gaattcttcc gttttcatca ggtcggtcaa ctcggtaaat ttcaagttga tacgcatatc
    14941 cggtttgtca gagccgtagt agaacatggc ttcggcgtaa ggcatacgcg ggaagtcgcc
    15001 taaatctacg tttaaagcat ctttgaagac ttgtttcgcc ataccttcgg tgatgtccat
    15061 aatttcatcc tcgttcaaga acgaggtttc caagtcgatt tgagtgaatt caggctgacg
    15121 gtcggcacgc aagtcttcgt cacggaaaca tttggtaatt tgatagtaac ggtcgaaacc
    15181 ggccaccatc aacagttgct taaataattg cggtgattgc ggcagcgcga aaaactcgcc
    15241 cggatgaacg cggctcggca cgaggtagtc gcgcgcgcct tccggagtgg agcgggtcag
    15301 catcggcgtt tcgatgtcga tgaagccttg cgcgtccaga taacggcgaa cgcccatagc
    15361 gacttgataa cgcaggcgca ggttgcgttg catcaccgga cggcgcaagt cgataacgcg
    15421 gttggtcagg cgaacgtttt cgctgatgtt ttcatcgtcg atttggaacg gcggcgtggc
    15481 ggcggcgttc aagacttcga tttctttggc aaggatttcg attttgccgg aaatcatttt
    15541 gtcgttggtc gtaccttcgg gacggttgcg tacgcggccg gtaatgctca aaacgtattc
    15601 gttgcgagcg gaatcggcag cggcaaacgc ttcgggcgtg tcggggtcaa tcacgacttg
    15661 gacgatgcct tcgcggtcgc gcaggtcgat aaaaatcaca ccgccgtggt cgcgccgacg
    15721 gtgtacccag cctttgacgg taacggtttg gtctaagtat tgctcactga tcagaccgca
    15781 atagttggta cgcataaaat caccttttat tgatttaaac tgaaaacaga aaatgccgtc
    15841 tgaacggcgg ctttattgtt gttcgggcaa atccgccttt tcagacggca taggtcctgc
    15901 caatgttttg acgggcaggt cgtcagggat gaccataccc agcgaaatca catatttcaa
    15961 cgcttcgtcc acgctcatat cgagttcgcg cacatcgctt ttctttacca taatatagta
    16021 accgccggtc ggattcggcg tggtcggaac atacacggaa agataatcgc cgtccttcgg
    16081 caatgcggcc ttaaccgcat tcgacacctg accggacacg aatgcgattg tccaaatacc
    16141 cgattgggga aacggcacga gtactggtgt tttaaacgaa cggctgctgt cggacagcaa
    16201 cgattcggat acttttttca cactcgaata gatggacttc acaaccggaa tccgccccaa
    16261 caagctgtcc cacgcggcaa gaatctgccg gcccaatacg tttgcggcaa acaatccggt
    16321 tacaaacaat acggcaatgg caacgataac gcccagcccc gggatattaa accccaaaac
    16381 atattgcggc cgccattgct tcggcagcag gttgacgagc tgatcggacg cggaaacgat
    16441 ataggaaacc acccaaaccg ttaccgcaat cggcagccag accaaaatgc ccgtaatcag
    16501 atattttttt aacgccttgg cagctttacc gccttcggcc gcaggttccg tcatcttgct
    16561 tgattccgac aaagtccgta caaaccgcac attatacgcg tttgcccgga ttcaaacgaa
    16621 atttttatcc cgccccgcca aaccgccggc ggcttcagac ggcacggcaa cttgatatac
    16681 cgtctgaaca cgcggttcag atgccgtcca agtcgttgaa catcaacccg ataccgatac
    16741 cgttctgctt gtggttgtag tcgatcagac tctcgccgta accgtggaat ccgcgtacca
    16801 cgcctttgag tttgccctta atcggaaacg tgtaggcggc ttcaatcgcg ccgtagcccg
    16861 ttttggggtt gtagcgcaat acggaataca cattctgcct gtcgttcagg cggtactgca
    16921 gcttcacgtc gccatacccc atatagtcgg caatatcggg attgtcgttt ttatcgccgc
    16981 tctgatcgaa cgcacgcacc cacacgcgcg gaatcaccgt caatttgccc cattccatgc
    17041 ctgccatggc ataaatcctg ttccacgaac gcgattcggg acggctctgt ccgttggact
    17101 ggtggacaaa acctgcaccg agcatacgca gcctgccgcc gaacggcaaa tctgccttca
    17161 caggctgggt caggaaaatt tcaggtttgt aatccgtatt gcggaacggc gcggatttcc
    17221 tgccttggtt gtaaatctgc caatcggatc tttgggtgta gccgaaccac agatccgcgc
    17281 gggttttaaa caaatcttcg gcaattttgc ttttgaacga aacctgcaat ttggtttccg
    17341 cacgtttctg ctgtccgaat ttttcctgta cagtcgtacc gcgcgtcggc gaacccgggg
    17401 catagttggg cgaattgttg taccagagcg gcataaggta catcggattg tgttcgcgta
    17461 cgcccaacag cccgcgcaaa tcgtttttgt ccaagtcgta catcaggctc aaaggcgtat
    17521 agatgtcggc ggtttcgccc gcactgtcgg caggaagcgc atccccgcct ttttcaacaa
    17581 caatgaccgc ctcgccctta tccaggctgc tgcggacggt ttccgtcaga ttgagtacgg
    17641 ctttcgactc ctgcccttcc taccctgccg aagacggaag ctgtgccgca aaaatcctgt
    17701 cgtaacacgc caaacgcgta acattgtccg tcaaagcggc gcattgcagc gcggtctctc
    17761 caaaagcgga tgccatcggc aacagtcctg tcaaaagaat atagcgcata ttccgtgtat
    17821 tcatctccgc ccccattgtc ggcatattgg ttttcaaacg gcattttata gcggattcgg
    17881 ataaaaaccg caccctttcc gccatttcgg gattttgccc ccgcaataca gaaaaccccg
    17941 aaaccgtcgg gcttcagggt tttccgctta tcgcgtatca accgccttgg cggttttgca
    18001 aaaatcaagc cagtgctttt acttttgcag acagacggct tttgtggcgt gccgctttgt
    18061 ttttgtggaa cacgcccttg tcggcgatgc ggtcgatgac tttgacggac tcttggtaaa
    18121 ccgcttgtgc ggcagcttta tcgcctgctt cgactgcttt caatactttt ttcactgcag
    18181 tgcggaatgc ggtacgcagg ctagcgttgt gggcgcgttg tttgacggac tggcgggcac
    18241 gtttgcgtgc ttgtgcgctg tttgccatat tggaatctcc tgaaagaaaa ataaattctg
    18301 taaacgcgca attttaaagg gggtttacat tatatgcaag ccttttctct ctatccgccc
    18361 gtcggacacc ctgacagcgc aaaaaaacgg caaaatcgtg cggacgtttt ctccctcttt
    18421 tgaaaacaag gtaatttttt aggaaaatca ggtcaatatc gctttgagtt tgcacaaatt
    18481 tactttacaa tgcccaaacc ttgcgccggg gtgtttatgg ggcgcggggt tttctttttt
    18541 tccaacctct ttcatcagga atctgcccgt atgaaaaaat ccgtattagc cgttttggcc
    18601 gcgctgtctc tggcagcctg cggcggcagc gaaaaaaacg ccgtgcaacc tcaagcagac
    18661 gctgcgtctg ctgccaatgc cgaagccgct gccaccgata ctctgaacat ctacaactgg
    18721 tcgaactatg ttgatgagag tacggtcgaa gactttaaaa aagccaacaa tctgaagctg
    18781 acttacgact tgtacgaaaa caatgaaacg ctggaagcca aaatgctgac cggcaaatcc
    18841 ggttacgatt tggttgtgcc gggcatcgcc ttcctgccgc gccaaatcga ggcgggcgcg
    18901 tatcaaaaag tcaacaaaga cctgattccc aactataaaa acatcgatcc cgaactcttg
    18961 aaaatgctgg aaaccgccga cccgggcaac cagtatgccg tcccctattt ctccggcgtg
    19021 aacacgattg cgattacggc gaaaggcaaa gagcttttag gcggcaagct gcccgaaaac
    19081 ggctgggatt tgctgttcaa acccgaatac acccacaagc tgaaatcctg cggcatcgcc
    19141 ctgtgggaca ccccgagcga aatgttcccg attttgctga actacttggg caaagacccc
    19201 aaaggctcga atcctgaaga tttgaaggcg gcggcggaag tgttgaagtc tatccgtccg
    19261 gatgtcaaac gtttcagccc gtccatcatc gacgagctgg cgcgcggcga catctgcctg
    19321 gcggcaggta acggcggcga cttgaacttg gcgaaagcgc gttctgaaga agtgaaaaac
    19381 aatgtcggca tcgaagtgtt gacgccgaaa ggtatgggat tctggattga gtcttggctg
    19441 attcccgccg atgcgaaaaa cgtcgccaat gcccacaaat acatcaacta cacgctcgac
    19501 cccgaaatcg cggcgaaaaa cggtatcgcc gtaaccttcg cgccggccag caaacctgcg
    19561 cgcgaaaaaa tgcctgccga gctggtgaac acccgttcca tcttcccgaa cgagcaggat
    19621 atgaaagacg gtttcgtgat gccgcaaatg agtacggatg cgaaaaaact gtcggtcagc
    19681 ctgtggcaga aaatcaaagt cggcaccaac tgatttgaag cattaaaaat gccgtccgaa
    19741 catcgttcgg acggcatttt atattggatt gaaatagaaa tatttatata gtggattaac
    19801 aaaaatcagg acaaggcgac
//
LOCUS       DAWFAD010000023        19703 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWFAD010000023 DAWFAD010000000
VERSION     DAWFAD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.023"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            41..454
                     /locus_tag="WGO49_000609"
     CDS             41..454
                     /locus_tag="WGO49_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0305750.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            500..1129
                     /gene="nth"
                     /locus_tag="WGO49_000610"
     CDS             500..1129
                     /gene="nth"
                     /locus_tag="WGO49_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ0305751.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            1267..2766
                     /locus_tag="WGO49_000611"
     CDS             1267..2766
                     /locus_tag="WGO49_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ0305752.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLIMRRGNTLFIALNLQ"
     gene            2966..4486
                     /locus_tag="WGO49_000612"
     CDS             2966..4486
                     /locus_tag="WGO49_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ0305753.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAIITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETAVSDGSRGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVDPSLIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            4543..5628
                     /gene="nagZ"
                     /locus_tag="WGO49_000613"
     CDS             4543..5628
                     /gene="nagZ"
                     /locus_tag="WGO49_000613"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ0305754.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTEEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHHNPEAVAHLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDRRSLSELEAADLAPFHIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGVGGLKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQEAQAFVAGLASPQDTAGGVKVGEAF"
     gene            5856..5932
                     /locus_tag="WGO49_000614"
     tRNA            5856..5932
                     /locus_tag="WGO49_000614"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5890..5892,aa:Pro,seq:cgg)
     gene            complement(6268..6906)
                     /locus_tag="WGO49_000615"
     CDS             complement(6268..6906)
                     /locus_tag="WGO49_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ0305755.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGIPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDMLKAEGYFL
                     CIETNGLNPAPPQIDYVATSPKACYAAKYEKSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(6903..7274)
                     /locus_tag="WGO49_000616"
     CDS             complement(6903..7274)
                     /locus_tag="WGO49_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ0305756.1"
                     /translation="MKLLSTLLVLLVTAEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGATVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(7543..7965)
                     /gene="queD"
                     /locus_tag="WGO49_000617"
     CDS             complement(7543..7965)
                     /gene="queD"
                     /locus_tag="WGO49_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ0305757.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            complement(7972..8487)
                     /locus_tag="WGO49_000618"
     CDS             complement(7972..8487)
                     /locus_tag="WGO49_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ0305758.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTEMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDHVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGKP
                     AEIGFQGYLPI"
     gene            complement(8515..9174)
                     /gene="queC"
                     /locus_tag="WGO49_000619"
     CDS             complement(8515..9174)
                     /gene="queC"
                     /locus_tag="WGO49_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ0305759.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGVLEYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            9279..9704
                     /locus_tag="WGO49_000620"
     CDS             9279..9704
                     /locus_tag="WGO49_000620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305760.1"
                     /translation="MPSEHAAYADLMASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGATVLEIVEDEDFEGDLGSWADSVKM
                     VRSSDGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            9719..10945
                     /locus_tag="WGO49_000621"
     CDS             9719..10945
                     /locus_tag="WGO49_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ0305761.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWR
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMTPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(10966..11246)
                     /locus_tag="WGO49_000622"
                     /pseudo
     CDS             complement(10966..11246)
                     /locus_tag="WGO49_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(11325..11459)
                     /locus_tag="WGO49_000623"
                     /pseudo
     CDS             complement(11325..11459)
                     /locus_tag="WGO49_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(11458..11916)
                     /locus_tag="WGO49_000624"
     CDS             complement(11458..11916)
                     /locus_tag="WGO49_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305762.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGTEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(12480..12683)
                     /locus_tag="WGO49_000625"
     CDS             complement(12480..12683)
                     /locus_tag="WGO49_000625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305763.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
     gene            complement(13247..14182)
                     /locus_tag="WGO49_000626"
     CDS             complement(13247..14182)
                     /locus_tag="WGO49_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0305764.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESIDYVDERKGVP
                     LAAMKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREFLVRNIDNIANDTEAFVNRYDLNRHMIYNTLLMVEGKQ
                     LDRLKQRSEKVLAHPTPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMA
                     AKETLSYFDFYLQPQIVTYAKIASMHGFDLGIDQEIAPRDLIVYDPLPADEYQDIFDF
                     MKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE"
     gene            complement(14185..14805)
                     /locus_tag="WGO49_000627"
     CDS             complement(14185..14805)
                     /locus_tag="WGO49_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305765.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQIELRVTAATDKQLLKAIGEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNPNGAGGYTQMSREWIKQVVKSLPD
                     GSPAKAVVLKANQNGKLKTAIAGVDRQTGKAVILSVKVPSKTNIRR"
     gene            complement(14884..15309)
                     /locus_tag="WGO49_000628"
                     /pseudo
     CDS             complement(14884..15309)
                     /locus_tag="WGO49_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            complement(15357..15629)
                     /locus_tag="WGO49_000629"
     CDS             complement(15357..15629)
                     /locus_tag="WGO49_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305766.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNDMD"
     gene            complement(15817..16305)
                     /locus_tag="WGO49_000630"
     CDS             complement(15817..16305)
                     /locus_tag="WGO49_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305767.1"
                     /translation="MKKDIFYCEQWSYGYKRLHKPFSEKQAEEKHIKGELYSAVIGSA
                     TQPEYVITLREEVGFFSVDFFDKFGRDYLTHQFQKYSNSNYYFLSMAVWRDYITLDSH
                     DLAEGYTYFFNENTDDCYVLKEDFINNERYEKTELYSQKDKVILFPKFGEYDLVLNPD
                     II"
     gene            complement(16302..18803)
                     /locus_tag="WGO49_000631"
     CDS             complement(16302..18803)
                     /locus_tag="WGO49_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0305768.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRIILSAADIAAENGK
                     IKLQSYGDQSYYAGQGELYTLERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATVFDAPKGSINIEAGRKLTLYAVEELNYDKLDSHKKRKFLGITYD
                     KVHDTSTQQMQTALPSRVVAESANLQSGWDTKLQGTQFETTLGGAVIRAGVGDQARAD
                     AKIILEGIKSSIHTETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTQIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGMTPAAAA
                     VVVIVVTVLTYGALSAPAAAGTAGAAGAGAGGAAAGTAAGTGVAAGTAATTGVAAGTS
                     AAAITTAAGKAALASLASQAAVSLINNKGDINHTLKELGKSSTVRQAATAAVTAGVLQ
                     GISGLNTQAAEAVSKHFHSPAAGKLTANLINSTAAASVHTAINGGSLKDNLGDAALGA
                     IVSTVHGEVASKIKFNLSEDYITHKIAHAIAGCAAAVANKGKCRDGAIGAAVGEMVGE
                     TLLDGRDVGKLSPQERQKVIAYSQIIAGSAVALVKGDVNTAANAATVAVENNSLLARR
                     RVNIRWTPRQELEHEYAILEIQAITNQIRRLDPKFNGIAIMRNPREPWTRHDVQTYRQ
                     YYNQLRESRGIPVLPIYRMRINNGNEFNRIMSSKYPYNELYVANPKSVTGHFRVDSYN
                     PATREIISRKFTQFSQIQESTGIGYIKEAVRKYSPGAVISNVPSTPASLRGRKLTGKL
                     ILEVPAQVNPIPQSVLRAAQEENVIIRDTTGRIYK"
     gene            complement(19053..19436)
                     /locus_tag="WGO49_000632"
     CDS             complement(19053..19436)
                     /locus_tag="WGO49_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305769.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
     gene            complement(19441..>19703)
                     /locus_tag="WGO49_000633"
     CDS             complement(19441..>19703)
                     /locus_tag="WGO49_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEZ0305770.1"
                     /translation="PETKIFKPISVDKYHNIASPPRGTLRNIDGEYKLLETIAQQLGN
                     NRNVSGRIDLFTELKACQSCSNVILEFRNRYPNIQLNIFTGK"
BASE COUNT         4677 a         4967 c         4679 g         5380 t
ORIGIN      
        1 caatccgtcg ccgatttttg cctgtttgag actgtttgca atgatgcccg cctacgccta
       61 cgccgcgctc gccttttccg ccttcacttc cgccaccctc ctgcccggca catccgaagc
      121 cgcctttgcc ctgttcgtcc gccgctttcc cgaacacgca tacggcgcgt tgctgtgcgc
      181 cggccttgcc aacggattgg gcagtatggt ttcctattgg atgggacgtt tgctgccttc
      241 ccgaaaaatg ccgtctgaaa aaacactgaa tctgatgcgg cgtttcggta tttggctgct
      301 tccgtttgcc tggctgcccg tcgtcggcga cgcactgccg cttgccgccg gctggctgcg
      361 gttgaatccg tggacaagcg ggctgatgct ggttatcggc aaaacggcgc gttacgcctt
      421 cattctgtgg ggaatgcaat attacgccgc ctgaaccgtg cgttataatg ccgaccgcac
      481 aaaccgactg cacaaaaaaa tgaacagaca catccgccaa gaaattttcg aacgcttccg
      541 cgccgccaac ccccatccga ccaccgagct gaatttcaac tcccctttcg agcttttaat
      601 tgccgttctg ctttcggcgc aggcgaccga tgtcggcgta aacaaggcga cggcgaagct
      661 gtttccggtt gccgatacgc cgcaggcgat gctggatttg ggtttggacg gcgtgatgga
      721 atacacgaaa accatcgggc tgtataaaac caagtccaag cacatcatgc aaacctgccg
      781 catcctgctg gaaaaataca acggcgaagt gccggaagac cgcgaggctt tggaatcgtt
      841 gcctggcgtg gggcgcaaaa cggcaaacgt ggtattgaac acagcgttcg gacaccccgt
      901 tatggcggtc gatacgcata ttttccgcgt gtccaaccga accaaaatcg cccccgggaa
      961 agatgtgcgc gaagtcgaag acaaactgat gcgcttcatt cctaaagaat ttctgatgga
     1021 cgcgcaccac tggctgattt tgcacggacg ctacacctgc aaggcactca aaccgcaatg
     1081 ccaaacctgc atcatcaacg atttgtgcga atatcccgcc aaagcctgac cgccgccgcc
     1141 cttcaaaatg aaacgcttga aaattcggac ggacgttgta gaatttcaac ctatgccgtc
     1201 tgaagccgac aaaatgcgct tcagacggca ttaaagccgg taccggcatc cgacaaagga
     1261 caaaacgtgt tcaaaaaata ccaatacctc gctttggcag cactgtgtgc cgcctcgctg
     1321 gcaggctgcg acaaagccgg cagctttttc ggtgcggaca aaaaagaagc atctttcgta
     1381 gaacgcatcg aacacaccaa agacgacggc agcgtcagta tgctgctgcc cgactttgtc
     1441 caactggttc aaagcgaagg cccggcagtc gtcaatattc aggcagcccc cgccccgcgc
     1501 acccaaaacg gcagcagcaa tgccgaaacc gattccgacc cgcttgccga cagcgacccg
     1561 ttctacgaat ttttcaaacg cctcgtcccg aacatgcccg aaatccccca agaagaagca
     1621 gatgacggcg gattgaactt cggttcgggc ttcatcatca gcaaagacgg ctatattctg
     1681 accaatacgc acgtcgttac cggcatgggc agtatcaaag tcctgctcaa cgacaagcgc
     1741 gaatataccg ccaaactcat cggttcggat gtccaatccg atgtcgccct tctgaaaatc
     1801 gacgcaacgg aagagctgcc cgtcgtcaaa atcggcaatc ccaaagattt gaaaccaggc
     1861 gaatgggtcg ctgccatcgg cgcgcccttc ggcttcgaca acagcgtgac cgccggcatc
     1921 gtgtccgcca aaggcagaag cctgcccaac gaaagctaca cacctttcat ccaaaccgac
     1981 gttgccatca atccgggcaa ctccggcggc ccgctgttca acttaaaagg acaggtcgtc
     2041 ggcatcaact cgcaaatata cagccgcagc ggcggattca tgggcatttc cttcgccatc
     2101 ccgattgacg ttgccatgaa tgtcgccgaa cagctgaaaa acaccggcaa agtccaacgc
     2161 ggacaactgg gcgtgattat tcaagaagta tcctacggtt tggcacaatc gttcggtttg
     2221 gacaaagccg gcggcgcact gattgccaaa atcctgcccg gcagccccgc agaacgtgcc
     2281 ggcctgcagg cgggcgacat cgtcctcagc ctcgacggcg gagaaatacg ttcttccggc
     2341 gaccttcccg ttatggtcgg cgccattacg ccgggaaaag aagtcagcct cggcgtatgg
     2401 cgcaaaggcg aagaaatcac aatcaaagtc aagctgggca acgccgccga gcatatcggc
     2461 gcatcatcca aaacagatga agccccctac accgaacagc aatccggtac gttctcggtc
     2521 gaatccgcag gcattaccct tcagacacat accgacagca gcggcggaca cctcgtcgtc
     2581 gtacgggttt ccgacgcggc agaacgcgca ggcttgaggc gcggcgacga aattcttgcc
     2641 gtcgggcaag tccccgtcaa tgacgaagcc ggtttccgca aagctatgga caaggcaggc
     2701 aaaaacgtcc ccctgctgat catgcgccgt ggcaacacgc tgtttatcgc attaaacctg
     2761 caataacaca cataatttaa ccaaaatatt ttttaaatga taaaatgccc gtcaataccg
     2821 attaacgggc attttaaaaa atcagttaat tgatttcaaa taagtatttt ccggaacaga
     2881 aaatactttc gcccattttg ccaacaaaat ccgaaccgtt gcggacagaa accgccccat
     2941 ccggacgctt aacaccccca taaccatgca gctgatcgac tattcacatt catttttctc
     3001 ggttgtgcca ccctttttgg cactggcact tgccatcatt acccgccgcg tactgctgtc
     3061 tttaggcatc ggtattctgg tcggcgttgc ctttttggtc ggcggcaacc ccgtcgacgg
     3121 tctgacacac ctgaaagaca tggtcgtcgg cttggcttgg tcagacggcg attggtcgct
     3181 gggcaaacca aaaatcctgg ttttcctgat acttttgggt atttttactt ccctgctgac
     3241 ctactccggc agcaatcagg cgtttgccga ctgggcaaaa cggcacatta aaaaccggcg
     3301 cggcgcgaaa atgctgaccg cctgcctcgt gttcgtaacc tttatcgacg actatttcca
     3361 cagcctcgcc gtcggtgcga ttgcccgccc cgttaccgac aagtttaaag tttcccgcgc
     3421 caaactcgcc tacatcctcg actccactgc cgcgcctatg tgcgtgctga tgcctgtttc
     3481 aagttggggc gcgtcgatta tcgccacgct tgccggattg ctcgttacct acaaaatcac
     3541 cgaatacacg ccgatgggga cgtttgtcgc catgagcctg atgaactatt acgcgctgtt
     3601 tgccctgatt atggtgttcg tcgtcgcatg gttttccttc gacatcggct cgatggcgcg
     3661 tttcgaacaa gccgcgttga acgaagccca cgatgaaact gccgtttcag acggcagccg
     3721 gggcagggtt tacgcattga ttattcccgt tttggcctta atcgcctcaa cggtttccgc
     3781 catgatctac accggcgcgc aggcaagcga aaccttcagc attttggggg catttgaaaa
     3841 cacggacgta aacacttcgc tggtattcgg cggcacttgc ggcgtccttg ccgtcgttct
     3901 ctgcacgctc ggcacgatta aaaccgccga ctatcccaaa gccgtttggc agggtgcgaa
     3961 atctatgttc ggcgcaatcg ccattttaat cctcgcttgg ctcatcagta cggttgtcgg
     4021 cgaaatgcac accggcgatt acctctccac actggttgcg ggcaacatcc atcccggctt
     4081 cctgcccgtc atcctcttcc tgctcgccag cgtgatggcg tttgccacag gcacaagctg
     4141 ggggacgttc ggcattatgc tgccgattgc cgccgccatg gcggtcaaag tcgatccctc
     4201 actgattatc ccgtgtatgt ccgcagtaat ggcgggggcg gtatgcggcg accactgttc
     4261 gcccatctcc gacacgacca tcctgtcgtc cacaggcgcg cgctgcaacc acatcgacca
     4321 cgttacctcg caactgcctt acgccttaac cgttgccgcc gccgccgcat cgggctacct
     4381 cgcattgggt ctgacaaaat ccgcgctgtt gggctttggc acgacaggca ttgtattggc
     4441 ggtgctgatt tttctgttga aagataaaaa acgcgccaac gcctgacccc tttccctgtt
     4501 cagacggcat atgccgtctg aagctttttg gagcaaaccg caatgaccgt cccccatatt
     4561 ccgcgcggcc ccgtcatggc agacattgcc gccttccgcc tgaccgaaga ggaaaagcaa
     4621 cgcctgctcg atcccgccgt aggcggcatc atcctcttcc gccgcaactt ccaaaacatc
     4681 gaacaactca aaacactcac cgccgaaatc aaagccctgc gcacacccga actcattatc
     4741 gccgtcgatc acgaaggcgg cagggtgcaa cgtttcatcg aaggcttcac ccgcctgccc
     4801 gcaatgagta cgctcggcga gatttgggac aaagacggcg cgtccgccgc cgaaaccgcc
     4861 gccggacaag tcggccgggt tttggcaacc gagctttccg cctgcggcat cgatttgtcc
     4921 ttcacgcccg tcttagattt ggactgggga aactgccccg tcatcggcaa ccgcagtttc
     4981 caccacaatc ccgaagcggt cgcccacctc gccctcgccc ttcaaaaagg tttggcaaaa
     5041 ggcggtatga aatcctgcgg caaacatttt cccggacacg gatttgtcga aggagacagc
     5101 catctggtct tgccggaaga caggcgcagc ctgtccgaac tcgaagccgc cgacctcgcc
     5161 cccttccaca ttatgagccg cgaaggtatg gcggcggtta tgcccgccca cgtcgtttat
     5221 ccacaagtgg acacaaagcc cgcagggttt tccgaaatct ggctcaaaca aattttgcgc
     5281 cgcgatatcg gattcaaagg cgtgattttc tcggacgatt tgactatgga gggcgcgtgc
     5341 ggtgtcggcg gcctcaaaga acgcgcccgc atttcctttg aggcaggttg cgacatcgtc
     5401 ctcgtctgca accgtcccga ccttgtggac gaactgcgcg aagatttccg cattcctgac
     5461 aatcccgctt tggcgcaacg ttggcaatat atggccaaca cgctcggcag tgccgccgcg
     5521 caagccgtta tgcagacggc ggatttccaa gaggcgcaag cctttgttgc cggccttgcc
     5581 tcgccgcagg atacagcggg cggcgtaaaa gtcggcgaag ccttttaaac cggctgcgtt
     5641 tcaacaatct gccgatttca tcaaccgttt gtcagggaaa ggcaatgatt ttttcggtca
     5701 ggtagaaagt tatcttattg aattacttaa agataatgat gattatcaac actgtattca
     5761 aacacactcc tttaaatccg cccgacacgc ggcaaaaagg caaaacggta taggcacgcc
     5821 gtattttttg cgctataatc acgccttact taattcggaa tgtagcgcag cccggtagcg
     5881 cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tcattccgac catattcaaa
     5941 ccgcctcggc atggattgct ggggcggttt ttcccttttg gcgtcaacag aaaaatgccg
     6001 tctgaaacag aatgctgttt cagacggcat ttcgttttcc ctaaccctgc catttgcgcg
     6061 cactggcaca gagtatcggc aaggttcaaa agaccatcgg aatttaggaa gccgagcttg
     6121 tacccaacgg cagcatctta ttcagtaagg ctccgtcctg attgggcggt ggaaatgccg
     6181 gcatttccac gcagaagcgg cacactttcc ccgtatgtca aacatcggta aaaccgtacg
     6241 ccgtcccttt gagttacact gcttaaacta ctctattccc gcccatttgt gcgtctgcac
     6301 gctcaactgc caatgcacgg atgcgtcggg gcgactgttt aaaataccga tttggcggat
     6361 ggtgtcgtag atgttcatcg caccgtcttg ctcacagggc gaaaggtagt aatgatgcgc
     6421 gcggattttg cgttccatat tttcacagaa cgcaaggaca tcgccgtcgg caacaatccg
     6481 cacttcgtcg gctgtttcga tacagctttt ttcgtatttg gcggcatagc aggctttggg
     6541 gctggtggcg acatagtcga tttgcggcgg cgcgggattg agtccgttgg tttcgataca
     6601 gaggaaataa ccttccgcct tgagcatgtc cagcagcata tcgagatgcg gctggatggt
     6661 cggttcgccg ccggtgatga tgatgttgcg ggcggcatag gttttcagac agcctaagat
     6721 gtcggacagg ctcatcatgc cgaatgtcaa ataatcggta tcacaccagc cgcaggcgag
     6781 gttgcatttg cccaagcgga cgaaaacggc gggtatgccc gtgttccagc cttcgccttg
     6841 caggctttca aagatttcga cgatgcggta ttggggattt tcgggggcaa cattgatttt
     6901 tttcataccg ccaacaccgc cgccgctgcc aaaaatgcgg gcaaaccttg tttgacgagt
     6961 atgcctttgt tgccggaaga gaacgcgccc cacgcggcgg caatcaggac gaagccgaga
     7021 aacagtaccg ttgcgccgta caccgcgtta tccggagcgg caaaccgcga ccacaccagc
     7081 ccgatgccga gaaagccgtt atacagccct tggttgccga acagggtctg cacgcgcttt
     7141 tgttccataa attcataagg cagcttgaat atttccgccg ctttttcgct gggaatctgc
     7201 gtcatttcaa gccaggcgat gtagaaatgt tcggcggtga caaggaggac taagagggtg
     7261 gagagaagtt tcatgggtat tccttttaat gggtgtattt gatatggagg gtcgtctgaa
     7321 aaaacgggga atccgtacag gagaaaaaac cgttttacgg taaattcaaa taaggacgct
     7381 ctaaaccgtc ctttccgcgc aggcgggaat ccaaagattt ggaattgctg caatttttaa
     7441 atattcctga aaaaccaagg tctggattcc cgcctgcgcg ggaatgacgc ggttgggcat
     7501 gtcttattgg aatttactat atcgatacgt tcaagatatt ccctactccc cttcatactc
     7561 cgcacacgat gtcggcgttt cccacaattt gacgcggcac acgttcagcc ccgcgttttt
     7621 cagacggtcg tacatttcga ccgccatatt ttcggcagtg gtgcggcagg gcaggcgcag
     7681 ggttttcatg ttccagcctt ccaagagtgc ggcgatttga gattcgcggc tgttgccgcc
     7741 gtggtagatg aaggcgtggt cgaaggggtc ggtaatgtgt tgtttgacaa tggctttcaa
     7801 gtcggtaaag tccatcacca taccgtcttt cgcgccgcct ttgataatgc cgtctgaaac
     7861 ggtgatttcg agtttgtagg tatgtccgtg caagttttgg catttgccgt catgcccgtc
     7921 gagcatatgc gaggagtcga aggtgaagat tttggtgatt ttcatgggtt ctcaaatggg
     7981 caaatagcct tgaaagccga tttcggcggg tttgccgtgc ggattcgggg tcaggcggat
     8041 atggaaattg ccgatgcggt cgagcgcaag caggttgtcc acgtctttta agttgtcttt
     8101 atgttgctga acatggtcgg tcaacagggt ttgcagggct ttttgtttgg cttcggcagg
     8161 cgtggcggcg gcgaacagcc cgaacgcgtg cgcctcggca agctgccccg cgcgataacc
     8221 gccgacattg gcaaaataca ggcgcggggc gtgttcagac ggcatttccg ttttgggcgt
     8281 ttcggatacg gcgatgtcgt aaccgtccgc ccattcgaca acctgccagc cgtcgatgtg
     8341 gattttgtcc gcatcgccga accacgcggc tttgagtgcg gggacggctt cacggtagtc
     8401 gtcgcacacg gcaaattgga tgtcgtgcac ttcgatattc gacctgccgg cattgccgcc
     8461 gaggtaaaac atatggagct tgggcatatc gtattcctta tggtttgcgc gtgttcagac
     8521 ggccttttta ctttccagat attccgccag cccgcgttcg cgcaagatac agctcgggca
     8581 ttcgcggcag ccgccgacga tgccgttata gcaggtgtgg gtttgctcgc ggatatactc
     8641 cagcacgccc atttcgtccg ccaacgccca cgtttgcgcc ttggtcagat acatcagcgg
     8701 cgtgtggatt tggaaatcat agtccatcgc caaattaagg gtaacgttca tggatttgac
     8761 gaacacgtcg cggcagtcag gatagccgga gaagtcggtt tcgcacacgc ccgcgatgat
     8821 gtgccgtatc ccctgccctt tggcgtaaat cgcggcatag agcaggaaaa gcgcgttgcg
     8881 gccgtctaca aaggtattcg gaacgccgtt ttcggcagtt tcgatggcgg cggtgtcgtc
     8941 catcagggca ttgtgcgtaa tctgccgcat caggctcaag tcgagtacgg tttgtttgac
     9001 gcccaaatcc tgcgcaatcc agcgggcacg ttccagctcg acggcatggc gttgcccgta
     9061 ttggaaagta atggcttgga cgttttcgcg cccgtagatt tggattgcct gaatcaggca
     9121 ggtggtcgaa tcctgaccgc ccgaaaagat gaccaaggct ttttggtttg acatatcgaa
     9181 tcctagtttt ggtaacgcgg gaggtttgcg aaccgcgaag ttggacagta aagacgcgga
     9241 ttatacaggc aaacgcgttc agacggcatc tgaacgccat gccgtctgaa cacgcggctt
     9301 atgccgactt gatggcgagt tccgccaagc gtttgctttg tgcgaaggcg atattgtttt
     9361 cttcggcggt caggacggga gccgctgtcg ggaatatctt ttttgacggc ttcgcagacg
     9421 gcggagactt tggggacggt gcgcaatacg gcttcgcaac cttcaacgct gtcgatgccg
     9481 cgagcgattc ggcgggcggg attgcgggtg ctaccgtttt ggaaatagta gaggacgagg
     9541 attttgaggg ggatttgggt tcatgggcgg attccgttaa aatggttcgg tcttcagacg
     9601 gcataacagt tgttccgatg ccgtctgaaa cagccgcagt tcaggcattt gttcctgacg
     9661 gcaaaattgg ctataacatc gggcatcaat gcccaactta ctgaccgggc aagaaattat
     9721 gaccttttta caacgtttgc aaggtttggc agacaataaa atctgtgcgt ttgcatggtt
     9781 cgtcgtccgc cgctttgatg aagaacgcgt accgcaggcg gcggcaagca tgacgtttac
     9841 gacgctgctg gcactcgtcc ccgtgctgac cgtgatggtg gcggtcgctt cgattttccc
     9901 cgtgttcgac cgctggtcgg attcgttcgt ctccttcgtc aaccaaacca ttgtgccgca
     9961 gggcgcggac atggtgttcg actatatcaa tgcgttccgc gagcaggcga accggctgac
    10021 ggcaatcggc agcgtgatgc tggtcgttac ctcgctgatg ctgattcgga cgatagacaa
    10081 tacgttcaac cgcatctggc gggtcaattc ccagcgtccg tggatgatgc agtttctcgt
    10141 ctattgggct ttactgacgt tcgggccgct gtctttgggc gtgggcattt cctttatggt
    10201 cggctcggta caggatgccg cgcttgcctc aggtgcgccg cagtggtcgg gcgcgttgcg
    10261 aacggcggcg acgctgacct tcatgacgct tttgctgtgg gggctgtacc gcttcgtgcc
    10321 aaaccgcttc gttcccgcgc ggcaggcgtt tgtcggggct ttggcaacag cgttctgtct
    10381 ggaaaccgca cgttccctct tcacttggta tatgggcaac ttcgacggct accgctcgat
    10441 ttacggcgcg tttgccgccg tgccgttttt tctgttgtgg ctgaacctgt tgtggacgct
    10501 ggtcttgggc ggcgcggtgc tgacttcttc actctcctac tggcggggag aagcgttccg
    10561 caggggcttc gactcgcgcg gacggttcga cgacgtgttg aaaatcctgc tgcttctgga
    10621 tgcggcgcaa aaagaaggca aagccttgcc tgttcaggag ttcagacggc atatcaatat
    10681 gggctacgac gagttgggcg agcttttgga aaagctggcg cggcacggct acatctattc
    10741 cggcagacag ggttgggtgt tgaaaacggg ggcggattcg attgagttga acgaactctt
    10801 caagctcttc gtttaccgtc cgttgcctgt ggaaagggat catgtgaacc aagctgtcga
    10861 tgcggtaatg acaccgtgtt tgcagacttt gaacatgacg ctggcggagt ttgacgctca
    10921 ggcgaaaaaa cagcagcaat cttgagtatt tttgaaactg tatttttatc ctaaagacat
    10981 tttcctatct gctgattcca ccgtttcctt tctgtgtgcc gccataattt ttatgataga
    11041 caagccattc aagtttgcat tgactgcttt ttcattggca atatccaaat ggctggtcgc
    11101 tgcatctaaa aataggattt tcggttcgca atataaggcc cgcgccaata cgatgcgttg
    11161 tttttgtccg cctgacagtg cgctgccata tcgccgatca aggtttcata gcccataggc
    11221 atggcgcaga tttcttcgtg aatcatagca aggcaggcgc aatgttcgat ttttcctcta
    11281 tccggttccg tatcgaaaaa acaaggggct gtactagatt agccctaaat cccacaccaa
    11341 tcccgcagga ttttaagctg ttgagacggt gtgccgaagt taaatcgaaa ttcgcattct
    11401 ttcaagaaca gcgggaaaga tttgcaatcg attccgttgt attttcgcaa gacacgttta
    11461 gtctagagtc tgtatattac atttattttt agggtctgct agccaatttc ttgttccctt
    11521 cattatttta tcttctgaaa gaaaattatt tttttccatg ctattaatat taatgatatg
    11581 atttttattt aaaataattt ttccatataa aatttccggt ttgtaatagc aagagtctga
    11641 agcaaatcct tgcccgtcaa tataagaaaa ataaatgtta tcatcctcct tataatattc
    11701 taaaaccaat ccggatatta ttttatcaaa attcccttca gttccatctt tcatgactaa
    11761 aatataatta gcaggccaac ctttatcgta aacatattca aactgatagt tcccgaaact
    11821 ctcgatatcc gaactaaaaa agaagaaaag caaattaata gccaatacaa aaacaaataa
    11881 gaacaaaaat agcaaaattt tcaacttagt taacaatatt tacctctcct ttaaattcaa
    11941 tcctgaaagg taccccttac cctgggctac caattatagt ttccatattt ctaaatattg
    12001 tttttacatt acttttttct ccccccaaag gaattcattt taaaatcatg cttttcaggt
    12061 gctaatcgat acttaccatt aacatcttta atcacagata tatttccatg tatagcccaa
    12121 cgtgaaaaat ctgagtatta tatacagtta taccttactg aaacaaagct agctgtacat
    12181 atctgtacca tatttttact acaaacaaaa aatatttata attattaagg ataagataaa
    12241 aacccccaac gccagattaa ttcttaataa tttcaaataa aaaatatttg agattttttc
    12301 ttttccaaat aatatattga aatatttaaa gttaaggtag cttgatttaa gtagaaaatc
    12361 aaaaaaacca ttaattaatt tttcttcttt aaatatttta tatgatatta taaaattaaa
    12421 taacattgcg aaaacatatg aaatcatcaa aataccaaat atatagtggg aatgcattat
    12481 tatttagctc cttcttttgg ctctgacaaa ttgacagtcc attgatatcc aatgcttgca
    12541 tttgcaattg ctgacgcacc tgcccctgtt ttccctgtta gtatcaatgg ttcttttctt
    12601 gcaagatgtg cacctgcaca accacctaat actaaaccac cacctattgt aaatccttcc
    12661 tgttgtatct ctaatgataa cattttcttc ttgtgccgcc cttaatacag attgtggaat
    12721 tggattgacc tgagcaggaa cttctaaaat aagttttcct tcaagctgtc ttcctcttag
    12781 cagattaggt gtacttggaa cattggaaat gacaaagtga gtgcgcaaag ccacctgaag
    12841 gcgatgtgtt tgaacctgtt gaaagcggcc aacaggctaa atgcgcccgc tgccgcctaa
    12901 aaggcagccc ggatgcctga ttatcgggtg tccgaggagg attaaggggg tatttgggta
    12961 aaattaggag gtatttggta cgaaaacagc cgaaatcctg tgtttgggtt tcggatgtcg
    13021 gggaagggct tttttgcaaa ggtctcaact catgttattg caaagatctc atgccttgtt
    13081 ttgccagcag gttgagttct tctccggcaa tcagcgtggt aaagccccgc cacaacacag
    13141 atctatgtac tgtggaaggg cttcaattta tagctttcaa aaatctttca gctttaagac
    13201 ggcctaaagt tgtaatctat aaaacaggag ctcagagttt tacggctcac tctcgcttcg
    13261 cgttaccaaa taccagctta tcggttttga acgtatagta atctatccaa tcctgcagat
    13321 attcatacgg gtaagacaag tcatactgtt tcataaaatc gaagatgtct tgatattcgt
    13381 ctgccggcag cggatcgtaa acaatcaaat ccctcggtgc gatttcttga tctatgccca
    13441 aatcgaaacc gtgcatggat gcgattttgg cgtaggtaac gatttgcggc tgcaggtaga
    13501 aatcgaaata ggacaatgtt tctttggcag ccatacgcgc ggttttttta tcgaaaagcg
    13561 gctctaaggc ggccttcatc gcctcggcat cctgttcggc gaaagcgagg aagaagcggt
    13621 aatcgtacaa ccgcttttgc agccatttgc taggggtggg atgcgccaag actttctcgc
    13681 tacgttgttt caaccgatca agctgcttac cctccaccat cagcagagta ttgtaaatca
    13741 tatgacggtt gaggtcgtat cggtttacga aggcttctgt atcgttggcg atgttgtcga
    13801 tattgcgcac caaaaactcg cgcagctgcg ggctgtcgct catcagcatc agaaacatcg
    13861 gatcttgaat gttgagcatg tcgcagggaa agaagaaggg ttcggggtct tcatcatcta
    13921 cactctgcaa aatacccaac tttcctgcaa cataagcata ttgcttgaat ttcttaagat
    13981 catgttcaaa tagataggca tgggaagccg cagcttcagt gtacattaaa atgtatttca
    14041 tcgctgcaag cggaacgcct tttctttcat ctacataatc tatagattcg gggcaaccgc
    14101 tattgaaatt agcagtattg cctatgatta cattagtaat atgttcatac catttttggg
    14161 tggtcatcat attgtgcccc attgttatct ccttatattg gttttagaag gaactttgac
    14221 agaaagaata acggccttac ctgtttgacg atcaacgcct gctattgccg tttttaattt
    14281 gccgttctga tttgctttta agacaactgc cttagcagga ctaccatcag gtaaactttt
    14341 tacaacttgt ttaatccatt cacgactcat ctgcgtatat ccacccgcac cattcggatt
    14401 cagctgtacc gtaccgttcc taatctgctt actttctaca atcaaaacaa cactaccatc
    14461 ggcagcctgc catacatgat caaaaccgtt atttccgccg tatttgccgc ccgaaagcac
    14521 tctgaagcca ttttgtttag ctaaagagtc aaataactgc tcggtcattt tacctgtcgt
    14581 gtcccttcct tcgccaatag ctttcagcaa ttgtttgtca gttgctgcag taacccttaa
    14641 ttcaatttga ccgattttga ttttggtttt actgacatag ccctgtgcag tggcaacggt
    14701 tgctgccttg tccaaggctt ttttcatgcc ttgtaaattt tttgccgctt tcgctacttt
    14761 ataggcctgt atcgattcac cgatacccgg aaccacaccc agcaaagcaa acaggtgatc
    14821 ggcagcggtc tgtgcttgta caaaactctg tatatcaccg ataattggaa taaatcctgt
    14881 ctgaagaagc aaaacttctc gatattcttc aggattttca cgtatccatt tttgttgttc
    14941 cttttgaaat tcatcacact ttgcaccaac gcatctagga tacaaagtat tattctccac
    15001 cgctatctgt gccgcattcg ccgctgtatt cacatccccg cctacaacgg cagacgcagt
    15061 gccggcaacc agttttgaat aggcagtaat cttcgcttta gattttttga tttcggtcgc
    15121 actcatacga ctgaaatcag tattcttaac caaagcctca ccaacaatct caccgactgc
    15181 agcgcctatc gcgccatcct gacacttgcc cttattcgcc gccgctgccg cacagcccgc
    15241 tatggcatgg gcaatcttgt gggtggtata tcgctgccag ccctctctgt tgataatctt
    15301 gcgtatgatt acgccgtttg atggctaatc gtacctacgc aggcttaaaa tttccatcaa
    15361 tccatatcat ttgagttttc atcttcataa tatggatgat caaacaaatc tccttcccaa
    15421 atacattcac tcgttatacc ttgggattcc atgaaaggaa cataagaata aaaatctttt
    15481 tcatattctc cataaaatat attcatacct ttgagccata ctgcatcgca ttcatcacac
    15541 agaataattt catcatgaag atatttaggt ttcgaatggt agagatagcc ttgttgacaa
    15601 gcaggacaca ttttcattct cttattcatg attactccat ataatcgatt tatttttgtg
    15661 ggtaagcagt agtgatttga ttgctgcctt tagtctgtac aacaatacgt atatagcaat
    15721 ctgataattt cctggttttt atcagcttta tcctcagatt ccaaacccaa taataaaagc
    15781 cgtttgaaat ttttttcaga cggccttaaa acttaattaa ataatgtccg gatttaacac
    15841 caaatcatat tcgccaaact ttggaaatag aattacctta tctttttggg aatataattc
    15901 tgttttttca tatcgctcat tattaataaa atcctctttc aaaacatagc aatcatccgt
    15961 attttcattg aagaaataag tatatccttc tgctaagtca tgagagtcta aagttatata
    16021 atctctccat acagccatag aaagaaaata ataattcgaa ttggaatatt tttgaaattg
    16081 atgggttaaa taatcccttc caaatttatc gaaaaaatct accgaaaaaa aacctacttc
    16141 ctctcgcaag gtaattacat attcaggttg tgtcgccgaa cctattacgg cactatataa
    16201 ttccccttta atatgttttt cttcagcttg tttctcagaa aaaggcttat gaagtctctt
    16261 ataaccataa gaccactgct cacaataaaa aatatctttc ttcatttgta aatccttcct
    16321 gttgtatctc taatgataac attttcttct tgtgccgccc ttaatacaga ttgtggaatt
    16381 ggattgacct gagcaggaac ttctaaaata agttttcctg taagttttct tcctcttagc
    16441 gaagcaggtg tacttggaac attggaaatg acagcaccag ggctatattt tctaacagcc
    16501 tccttgatat aaccaatccc cgtactttct tggatttgag aaaattgggt aaattttctt
    16561 gaaataattt cccttgttgc aggattatac gaatctaccc taaaatgtcc cgttaccgat
    16621 ttaggattgg ctacataaag ctcattataa gggtattttg atgacatgat acggttaaat
    16681 tcattgccgt tgtttatcct cattctataa attgggagaa caggaatgcc tctggattcc
    16741 cttaattgat tataatattg cctgtatgtt tgtacatcat gtcttgtcca cggctctcta
    16801 ggattcctca taatagcaat cccgttaaat ttcggatcca gccttcggat ttgattggta
    16861 atggcctgga tttcaagaat ggcatattca tgttccaatt cttgtcgcgg agtccaacgt
    16921 atatttaccc tcctgcgagc taaaagacta ttattctcca ctgccacagt agccgcattc
    16981 gccgccgtat tcacatcccc tttaaccaat gccactgcgc tgcctgcgat aatctgcgag
    17041 taggctatga ctttttggcg ttcttggggt gacagtttgc ctacatcgcg tccgtccaac
    17101 agggtttctc ccaccatctc gccgactgcc gcgccgattg cgccgtcccg acatttgcct
    17161 ttatttgcta ccgctgccgc acagcccgct atggcatggg caatcttgtg ggtaatgtag
    17221 tcttcgctga gattaaattt gattttgctc gctacttctc cgtgtacggt actgactatc
    17281 gcacccagtg cggcatcgcc caagttgtct ttgaggctgc cgccgttgat ggcggtatgg
    17341 acacttgcgg cagcggtgct gttgatcagg ttagcggtca gtttgcctgc tgcgggactg
    17401 tgaaaatgtt tgctgacggc ttcggctgct tgggtgttca gcccgcttat gccctgcagt
    17461 acgcctgcgg ttacggcggc ggtggcggcc tgtctgacgg tgctgctttt gcccagttct
    17521 ttcagggtat ggtttatgtc tcctttgttg ttgatgaggg aaactgcggc ttggctggcg
    17581 agactggcca gtgcggcttt gcctgcggct gtggtgatag ctgcagctga tgtgcctgct
    17641 gctactccgg ttgtggctgc cgttcctgct gctactccag ttccggctgc cgttcctgct
    17701 gcggctcctc ccgctcctgc gcctgccgcg cccgccgttc cggcggctgc cggggcggac
    17761 agtgcaccgt aggtcaatac ggttacgacg ataacgacga cagctgctgc tgcgggtgtc
    17821 atgccctcct gtttgtagtc ccatttatcg taagcaagct gcacctgatt ccagttgatg
    17881 tttttcgcaa cttgaagttg tttcaaataa gcatactcgg gctgcttggt gagggtttcg
    17941 atttgggttt tcagattgcc tttcggaatg tcgacaatgt aaccgccggg agcagagagt
    18001 acgggcgcaa cggggccggt gaaacttggc agttgcaggg tttcgatgtt gctgccgcgt
    18061 cctgcctgtt tttgccagag ggcggatttg ctgctgctta cggtttctgt gtggatgctg
    18121 cttttgatcc cttcgaggat aatcttggca tcggcccgtg cctggtcgcc tacgcctgca
    18181 cggatgaccg cgccgcccag cgtggtttca aactgcgtac cttgtaactt ggtatcccaa
    18241 cctgattgca gattagctga ttcagccact acacggcttg gtaaggcagt ttgcatctgt
    18301 tgggtagagg tatcatgtac cttatcataa gtaatgccga gaaatttgcg tttcttatga
    18361 ctgtctagtt tgtcgtagtt gagctcttct acggcataga gtgtcaattt ccgcccggct
    18421 tcgatgttaa tgctgccttt gggggcatcg aatacggtgg cgtaggcgtc gatgctgcca
    18481 ccagatttga tgtcgatacc ttgggatgcg ctgaggttta ctgcgtcggg cttggcgttt
    18541 ttgtgttctt tgacttcggt aatgtgtttg cggttgtacc atttgccggt tttgtagctg
    18601 cggcgttcta aggtatagag ttcgccctgc ccggcgtaat aggattggtc gccgtaggat
    18661 tgcagtttga ttttgccgtt ttctgcggct atatcggcgg cactcaaaat gatgcggctg
    18721 ttctcattgg catacggtgc gctaatgctc acaccggttt tacccgaaag ttctgcagca
    18781 agcgtagggg gtgtcttcag cacgcacgcg tctgggcagt atatagggaa tctgaatatt
    18841 tacttgaata acaaatgccg tctgaaaaat tgtgagcttt tcagacggca ttgagccgta
    18901 aatcatggaa cgcgtgcgtg ctgaagcaca caccttacgc atggatttta ggtttcatgc
    18961 aggctacagc ttgctgcggc ggcggtataa aagtattttt agtgatttac cgtttttggt
    19021 aattttgccc gacggggtta aaaatacagt tgctactctt gctgcatgat attggcaaga
    19081 tttaatttaa atgtttgttg aatcttgttt acatccttag caaaaatatg atagtcactc
    19141 gaagaataga tagattttat tctatccaaa ttagtttgtt gataacattt tttcatttcc
    19201 gcgatgaaaa aattatcttt tcctctttca ttgagacagt tattttctaa cagatagatt
    19261 gcccatgctg taaaaataaa tgtactatct tccggatatt cagataaata aatttcgaat
    19321 tcaattatag aatattcagc tgcttcaatt aatgttatat ctaaatctct gacaaatttt
    19381 tccgttatat ttttaaataa tttcttgaat tctatataat tttttatttt ttccatgatt
    19441 ctattttcct gtaaaaatat ttaattgaat atttggatag cgatttctaa actctaaaat
    19501 aacattgctg caagattgac aggcctttaa ttctgtaaat agatcaattc tacctgatac
    19561 attacgatta tttccgagtt gctgtgctat agtttcaagt aatttatatt ctccatctat
    19621 atttcttaat gttcctctag gaggagaggc aatattatga tatttatcaa cagatatagg
    19681 tttaaaaatt tttgtttcag gta
//
LOCUS       DAWFAD010000024        19609 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWFAD010000024 DAWFAD010000000
VERSION     DAWFAD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19609
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.024"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(368..922)
                     /locus_tag="WGO49_000634"
     CDS             complement(368..922)
                     /locus_tag="WGO49_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305771.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            complement(1007..1507)
                     /locus_tag="WGO49_000635"
     CDS             complement(1007..1507)
                     /locus_tag="WGO49_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305772.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            1911..3107
                     /locus_tag="WGO49_000636"
     CDS             1911..3107
                     /locus_tag="WGO49_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ0305773.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTKGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINTPETLNHVREQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DEAYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLNDEDMAEGLRRLEHALTEFA
                     ATSDNP"
     gene            complement(3210..4454)
                     /gene="clpX"
                     /locus_tag="WGO49_000637"
     CDS             complement(3210..4454)
                     /gene="clpX"
                     /locus_tag="WGO49_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ0305774.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKGDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLKGL
                     KKVVVGKAVIEEGREPELVFEP"
     gene            4645..5016
                     /gene="rbfA"
                     /locus_tag="WGO49_000638"
     CDS             4645..5016
                     /gene="rbfA"
                     /locus_tag="WGO49_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ0305775.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKTPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            5049..5981
                     /gene="truB"
                     /locus_tag="WGO49_000639"
     CDS             5049..5981
                     /gene="truB"
                     /locus_tag="WGO49_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ0305776.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFHAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIIATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKPRDITVYAIDI
                     AEFDAPKAVISVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFEEDLPSDTPVRVYT
                     ENGRFVGLAEYQKEICRLKALRLMNTAVKNTGCV"
     gene            6272..6748
                     /locus_tag="WGO49_000640"
     CDS             6272..6748
                     /locus_tag="WGO49_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305777.1"
                     /translation="MKADIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPAI
                     IGLLLGNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDSSDIVLDFPFKDCVLNGGQSSEEGEEIYFKHSNSQPASQPASQPASQPANYTLN"
     gene            6976..8454
                     /locus_tag="WGO49_000641"
     CDS             6976..8454
                     /locus_tag="WGO49_000641"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0305778.1"
                     /translation="MKLIYIDPPYYFVKKKQQDSFGYNTNFKLSTWLTFMKNRLSIAK
                     ELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFIGNLPTIMNLKGNNDEYAFAGTHE
                     YTLVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGFYKKGAPMRATGTEDKREDRPEMF
                     YPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQKLKEKYENLGYNFILPIADKQWG
                     RWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRPELDDLPTKKPKTIFYKPEYSSGN
                     GTEQMKNLFGEKAFKNPKPEELIQDFITITTNESDIVLDYHLGSGTTAAVAHKMNRQY
                     IGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQGGGEFVYAELAPFNETAKQQILA
                     CEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQEPEFQSLALDEQKQMVLEMLDLNQ
                     MYVSLSEMDDEQFAGCLNDDDKALSREFYQSAKNQAEKKDGE"
     gene            8444..11218
                     /locus_tag="WGO49_000642"
     CDS             8444..11218
                     /locus_tag="WGO49_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0305779.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHAKFLFTEKILQGDTVIPIRKVEMFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHKLNLVMLGDEAHHLNAQTKGKKQDELDLEVELKA
                     NAGNAEIERKGWEHMVLELLLNKNGNPGQNVLLEFTATLPENADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLNKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     IDESKADYSAFLNWAENVQADDFEFLDTFSGSLNNSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWVKQNYQKHNVIITNSETNKNKTEKTDSETEKLLNNLEAADNPIRAIFTV
                     DRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEG
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNKDFRELLIWANKKIPNPNAKSNNADSLQANPQTLSFQIHGNQLLQETQ
                     FTADENDETARQIGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLGQDKQVRPDDKLAGCLKILEMVEKHLNESDMPFIGT
                     KEFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSKYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEK"
     gene            complement(11367..12539)
                     /locus_tag="WGO49_000643"
     CDS             complement(11367..12539)
                     /locus_tag="WGO49_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ0305780.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            12828..13274
                     /gene="iscR"
                     /locus_tag="WGO49_000644"
     CDS             12828..13274
                     /gene="iscR"
                     /locus_tag="WGO49_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ0305781.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            13303..14517
                     /locus_tag="WGO49_000645"
     CDS             13303..14517
                     /locus_tag="WGO49_000645"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ0305782.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWANNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYINGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            14778..15164
                     /gene="iscU"
                     /locus_tag="WGO49_000646"
     CDS             14778..15164
                     /gene="iscU"
                     /locus_tag="WGO49_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ0305783.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            15251..15571
                     /gene="iscA"
                     /locus_tag="WGO49_000647"
     CDS             15251..15571
                     /gene="iscA"
                     /locus_tag="WGO49_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ0305784.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(15531..15752)
                     /locus_tag="WGO49_000648"
     CDS             complement(15531..15752)
                     /locus_tag="WGO49_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ0305785.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKRGSFRRSRRSL"
     gene            15834..16334
                     /gene="hscB"
                     /locus_tag="WGO49_000649"
     CDS             15834..16334
                     /gene="hscB"
                     /locus_tag="WGO49_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ0305786.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            16432..19182
                     /gene="gyrA"
                     /locus_tag="WGO49_000650"
     CDS             16432..19182
                     /gene="gyrA"
                     /locus_tag="WGO49_000650"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ0305787.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            19339..19609
                     /locus_tag="WGO49_000651"
                     /pseudo
     CDS             19339..19609
                     /locus_tag="WGO49_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5489 a         4955 c         4599 g         4566 t
ORIGIN      
        1 ccttttccgc tttggcaacg attgaaaatt tccgtaaatt caaatatcta gattccttcc
       61 tgcacgggaa tgacacggaa gggtttcaga tgcagggtgg gcattcctgc ccacccaatc
      121 ccgcccttgc aacggtgggc aagaatgctc gccctatggt ctgacggttc gatatgatgc
      181 cgtctgaaaa cccaacggcg gcatgacaat gccgccctgc caacgcacgt aaatcagaat
      241 tgccatcccg acatcaaacg cttggaaaca aaatgccgtc taaaaatcaa acggcaacat
      301 aacaatgccc ctaacaaatg caaaaatgcc gtctgaaagc ccttcagacg gcattggcac
      361 gccggattta ccgccttctg ccgaaaccgc gcatggcggg gcggcggtaa ttggcgggcg
      421 ggcgcgttgt cgggcggtaa cgctgcgcct gcgccgcctg ttgttttgca cggaggctgc
      481 gcgtgttcaa atccctgctg gtgcgcgcat tgggatgtgc ggactgatgg taggctgcac
      541 gcgcgcgccg ggcgacggga ctgtcttggt tgcctgcccg tgtgaatttg tttgccagcg
      601 cgttgccgat aaacgcgcct gccgccgcgc cgactaggct ttgcagcagc cagcttcctg
      661 tcgattggtc gtaaatatac tgctgcccgt ctttaccggt aacgggttgc ccgttgttgc
      721 cgtttgcctg tgcttcggca ggaatggtgt ctttgactgc ttcgggagtc agttggtaaa
      781 ccgtatcgtc tgcctgctgt gcgagctgct gttgcagggc ttcaatctgt ttctgctgct
      841 gttcgagccg cgcctgcgtg tcgtcttggc aggcggcgag tgcgaatgtt gcgataagcg
      901 cggaggcgat gatttttttc atgtgtgtcc tgtttgggtg gaaaatcggt tttattgtat
      961 cgccgtcggg aattttggca agcattctgc cgacaaatcg tgatgtttac aggggcaggg
     1021 tgtgcagttt gcggacaaat gcgaggctgt tggcgactgg gttgcctttg ttttcgactt
     1081 cgtgttcggt ttctacggtc agcaggcggc ggtttttgtg tttgttcagg ctcaattcgg
     1141 cttgtgcggg tgtgaaaaac aggcggtgtt tttcggcaaa gcggcgggcg cggctgttgg
     1201 cggttttcaa aatctgaagc agcgatacgt ccagatggaa gcgtcgcacg cccaatacga
     1261 gaatccgtgc ggcaaaaaaa tcggcggcat cggtaaactc ggcggggctg ctgcggctga
     1321 agtcgtgccg ttcggcggct atcagggcgg caacagtgcg gatttgcgga atcatcgatt
     1381 cgctgataag tgtgtcgtcc cgccctgcat cgagaagcat gggggaaaaa tcctgtgcat
     1441 catcgacaat aatgctacaa gtgtgcaggg tttcgttttg tgcggcggtt tggggcattg
     1501 cattcatggt cattttcctg attctgtcgt gtgttgccga atcgggcgac ctgtgtgaag
     1561 gtaacaaaaa agccgccccg ttttcgagcg gcctgttttg cgtatgggat ggatttcaag
     1621 caagcgcaaa aaagtaccgc acgtctgtgt ggtaccaata gcaataagcg gttgtaaatt
     1681 ttttgccttg catgatgaaa tgccgtctga agataaaaat attggggaga ttctaaatca
     1741 aaacgctgcc gcgcctcaag cattttatcg aaattttttt gatttttcat ctatccgatt
     1801 gaaaatattt cggtttattt ttaccgctgc ccgatattgt cggcaatttc cctttatctg
     1861 ctttgaaaaa cggtgcataa tcccgagcaa aaccgcaatc aggagcaatt atgcaaaact
     1921 atctgacccc caatttcgcc tttgccccga tgattcccga acgcgcttca ggcagccgcg
     1981 tttgggatac gaaagggcgt gaatatattg atttttcagg cggtatcgcc gtcaatgcgc
     2041 tgggacactg ccaccctgcc cttgtcgatg ctttaaacgc gcagatgcac aagctgtggc
     2101 acatttccaa tatctatacg acgcgtccgg cgcaggaatt ggcgcaaaaa ttggttgcaa
     2161 acagttttgc cgacaaggtt tttttctgca actcgggcgc ggaagcgaat gaggcggcgt
     2221 tgaagctggc gaggaaatac gcccgcgacc gtttcggcgg aggaaaaagc gaaatcgtcg
     2281 cctgtatcaa cagttttcac ggacgcacgc tgtttaccgt gtccgtcggc ggtcagccga
     2341 aatacagcaa ggattatgca cccctgccgc aaggcattac gcacgttccg ttcaacgata
     2401 ttgccgcgct ggaagctgcc gtcggcgaac agacctgcgc ggtcatcatc gagccgatac
     2461 agggcgaaag cggcatcctg cccgccactg cggaatattt gcaaaccgcg cgccgtctgt
     2521 gcgaccggca caatgcgttg ttgattttgg acgaagttca aaccgggatg gggcatacgg
     2581 gcaggctgtt tgcctatgaa cattacggca ttgttcccga tattttgagt tcggcaaaag
     2641 ccttgggctg cggctttccg atcggcgcga tgctggcgac agaaaagatt gccgccgcct
     2701 tccaaccggg cacgcacggc tcgactttcg gcggcaaccc gatggcgtgt gcggtcggca
     2761 gccgcgcatt cgacatcatc aatacgcccg aaactttaaa ccatgtccgt gaacaggggc
     2821 agaaacttca gacggcattg ctggatttgg gcaggaaaac gggcttgttc tcacaagttc
     2881 gcgggatggg gctgctactc ggctgcgtgt tggacgaagc ctatcgcgga cgcgcatccg
     2941 aaatcaccgc cgccgccttg aaacacggcg tgatgatttt ggttgcgggt gcggacgtat
     3001 tgcgtttcgc gccttcgcta ctgttgaacg atgaggatat ggcggaaggt ttgcgacgtt
     3061 tggaacacgc gctgacggaa tttgccgcga catcagacaa tccgtaaaac tcaaatgccg
     3121 tctgaaggcg ggaaggcttc agacggcatc aaaaacaaaa aaccgcttcg gaaacgtggt
     3181 tcgacgttcc gaagcggttt tgtttgccat cagggctcga acaccaattc cggttccctg
     3241 ccctcttcga tgactgcctt accgaccacc acttttttca agcctttcaa atcgggcagg
     3301 cggtacatcg tatccaacag gcagcgttcg acgatggaac gcaggccgcg cgcgccggtt
     3361 ttgcgctcca ttgcctgacg cgcgatggaa cgcaatgcgc cttcttcaaa ctccagttcg
     3421 acattttcca taccgaacaa ggcttgatac tgcttgacca aagcattttt cggctcggtc
     3481 aaaatattaa tcagcgcgtc ctcatccagt tcttctaacg ttgcaatcac aggcaaacgt
     3541 ccgattaatt ccggaatcag accgaatttg attaaatctt ccggttcgac gatgccgaac
     3601 agcttggtaa tgtcggcatt ttcgtccttg ctgtgaacgg acgcaccgaa accgataccg
     3661 cctttctcgg tgcgctggcg gatgactttt tccaagcctg caaacgcacc gccgcagata
     3721 aacaggatgt tggtggtatc gacgttgata aattcctgat tcggatgctt gcggccgcct
     3781 tggggcggaa cacttgccac cgtaccttca atcagtttca gcaaggcttg ttgcacacct
     3841 tcgccggaca cgtcgcgcgt gatggacgga ttgtcgcctt tgcgtgaaat tttatcgatt
     3901 tcgtcgatat agacgatgcc gcgctgggct ttttcaacat cgaaatcaca tttgcccaac
     3961 agtttggtga tgatttgctc gacatcttcg ccgacataac ctgcttcagt cagcgtggtt
     4021 gcatccgcca tcacgaacgg tacgtccagt ttacgcgcca aagattgcgc cagcagggtt
     4081 ttacccgatc cggtcgggcc gataagcagg atgttggatt tcgacaattc gacattagct
     4141 ccggctttcg ggtggcgcag gcgtttgtaa tggttgtaca ccgacaccgc caaggctttc
     4201 ttggcttgtt cctgaccgat aacgtggtcg ttgaggttgg cgacgatttc ggcgggcgtg
     4261 ggcagcttgc cggattcttc cggctcccct ccggcacttt ccgaaggtgt gccgtcattt
     4321 ccgtcttcat gcaatatttc aatacagttt gagacgcatt cgtcacagat aaaggcgttt
     4381 tcgccctcaa ttaaatgttt gacgtgtgat ttggattttc cgcaaaagga acaggtacgg
     4441 ttttcgttgg acatggcttt ctttacaatg tatgcgttac agaaaacggc acgtgccgtt
     4501 cgggttgcca agtataataa ctatatccgt tcttatcaat gtattacctt aaaatcccgc
     4561 cgattaggct ataatacgcc ctttcgcaac cgccccggcg gcaaaaatgc cgtctgaaac
     4621 caaatctgaa atctgaggat attcatgaga aaaccccaac gcggctacgc ccgccaagac
     4681 cgtgtcaaag aacaaatcat gcgcgagctt gccgaactcg tccgtaccgg actgaaagac
     4741 ccgcgcgccg gcttcattac cgtcaacgaa gtcgaagtta cccgcgatta cagccacgcc
     4801 accgtgttct acaccatttt aaaccaagac gcgcgcgaaa ttacggaaga agtgctggaa
     4861 cacgcgcgcg gacacctccg cagcgaattg gccaaacgca tcaagctgtt caaaacgccc
     4921 gaactgcatt tcaaatacga cgaatctttg gaacgcggtt tgaacctgtc cgcccttatc
     4981 gaccaagtag cggcggaaaa accggttgaa gactgacgga tatgcccatg ccgtccgaac
     5041 atcgaaccat gaatacaggc aaaccccaaa aacgtgccgt caacggtgtt ttgctcttgg
     5101 acaaacccga aggcctttcc agcaacaccg cgctgcaaaa agcgcggcgt ttgtttcatg
     5161 ccgaaaaagc cggacatacc ggcgtgctcg accctttggc aaccggactt ttgcccgtct
     5221 gcttcggtga agcgaccaag ttcgcccaat acctgctgga tgccgacaaa gcctacaccg
     5281 ccacgctgaa actcggcgaa gccagcagca cgggtgatgc cgaaggcgaa atcattgcca
     5341 ccgcccgcgc cgatatttcc ttagccgaat ttcagacggc ctgccaagca ctgacaggca
     5401 acatccgcca agtgccgcca atgttttccg cgctcaagca cgaaggcaaa ccgctgtacg
     5461 aatacgcccg caaaggcatc gtcatcgaac gcaagccgcg cgatattacc gtttacgcca
     5521 tcgatattgc cgaatttgac gcacccaaag ccgtgataag cgtgcgttgc agcaaaggca
     5581 cctatatccg caccctcagc gaagacatcg ccaaacacat cggcacgttc gcccacctga
     5641 ccgccctgcg ccgcaccgaa accgccggct ttaccatcgc ccaaagccac acgctcgaag
     5701 ccttggcaaa tttgaacgaa acagaacgcg acagcttgct gctgccctgc gacgtattgg
     5761 ttgcccattt tccccaaacc gttttaaacg attatgccgt ccatatgctc caatgcgggc
     5821 aacggccgcg tttcgaggaa gacctgcctt ccgatacgcc ggtacgcgtt tacacggaaa
     5881 acggccgctt tgtcggactg gcggaatatc aaaaagaaat atgccgtctg aaagccttgc
     5941 gcctgatgaa cacggcggtt aaaaatacgg gctgtgtttg aataatgtgt tgatatagtg
     6001 gattaaattt aaaccagtac agcgttgcct cgccttgccg tactatttgt actgtccgcg
     6061 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ttccgcaaaa tcacgacaca
     6121 ctcggacacc caccccgctt attgcaactt tgcgaacgcc cccggaaaca gcaaagataa
     6181 caaatgattg attttattag tatttatttg caaaaccatt tgccgttaca caagaatggc
     6241 acattaaaat gactgatgag gatttataac gatgaaggca gacattcaaa ccgaattaac
     6301 ccaagccctg ctatcacacg aaaaagtatg ggcgaacgaa gaaaaaacca ttttagccaa
     6361 aaacattctg ttggatttgg tggaaaaaac cgacccggcc attatcggtt tgttattggg
     6421 gaatgatgag ttaaaacgcc atttttttgt ggaagtgaat ggtgttttgg tgtttaaatt
     6481 gcaggatttt cgttttttct tggacaaaca cagcgtcaat aattcctaca caaaatacgc
     6541 caaccgcatt ggtttgacgg acggcaaccg ctttttgaaa gacagttcgg atattgtgtt
     6601 ggattttccg tttaaagatt gcgtattgaa tggcgggcaa agcagcgaag aaggcgaaga
     6661 gatttatttt aaacacagta atagccagcc agccagccag ccagccagcc agccagccag
     6721 ccagccagcc aattatacac taaattaacc cgaaaaagac aagaaatctt ttttaatcaa
     6781 acgcttgctt ttgatgaaat tgaccggctt tttgacgcaa aagcattctc aaaattctct
     6841 cgccacaccg cagacggcaa acaagcggtt ggcgaaatca aacgacattc agacggcaca
     6901 ccagccgaaa atctcattat caaaggcaat aatctgattg ccctgcattc gcttgccacg
     6961 cagtttaaag gtaaagtgaa gctgatttat attgatccgc cttattattt tgttaaaaag
     7021 aaacaacaag atagttttgg ttataacact aactttaaat tatctacttg gcttacattt
     7081 atgaaaaatc gtttatcaat tgccaaagaa ttattaactg atgatgggat tattgttatt
     7141 tccattgatg atgacggaaa tgcttattta aagattttgt tagatgaaat ttttggattt
     7201 gaaaatttta tcggaaattt acccactatt atgaatttaa aaggtaataa tgatgaatat
     7261 gcttttgcag gcactcacga atacacacta gtctttgcta aaaataaaga taaatctact
     7321 ttttatgaat ttccaataga tgaagataat tttttagaga aatgggaaga agatgaaatt
     7381 gggttttata aaaaaggagc tccaatgcga gcaacaggaa ccgaagataa aagagaagat
     7441 agacctgaaa tgttctatcc ttttttggta aaaaataaca cagttagtac aattacagat
     7501 gaagaatttt ctcaaatcta taataaagat ttagaagtat tcaatgatga ttttattcaa
     7561 aaactaaaag agaaatacga aaatcttgga tacaatttta ttttacctat tgctgataaa
     7621 caatgggggc gttggcgttg ggggtattca ataaagaata aggcaaggct gcagacggat
     7681 attattgtta gccaatctaa aaatggaatt tccttgtata aaaaacaaag acctgaatta
     7741 gatgatttac ctacaaaaaa acctaaaact attttttata aacctgaata cagtagtggt
     7801 aatggaactg aacagatgaa aaatcttttt ggtgaaaagg catttaaaaa tcctaaacca
     7861 gaagaactta tccaagattt tattaccatc acaaccaacg aatccgacat cgtcttagac
     7921 taccatcttg gtagtggcac aaccgccgcc gttgcccaca aaatgaaccg acaatatatc
     7981 ggtattgaac aaatggatta tattgaaacg cttgccgttg aacgcttgaa aaaagtgatt
     8041 gatggcgagc aaggcggtat ttccaaagcc gtgaattggc aaggtggtgg cgagtttgtt
     8101 tatgccgaac ttgccccatt taacgaaacc gcaaaacaac aaattttggc ttgcgaagat
     8161 tcagacggca tcaaaacgct gtttgaaggt ttatgcgaac gctatttctt gaaatacaac
     8221 gtcagcgtaa atgaatttag tcaaatcatt caagagcctg aatttcaatc cttggcatta
     8281 gacgaacaaa aacaaatggt gcttgaaatg ttggatttaa atcaaatgta tgtttcatta
     8341 tccgaaatgg atgatgagca atttgcaggt tgcttaaatg atgacgataa agcgttaagc
     8401 cgtgaattct atcaatcagc aaaaaatcaa gcggagaaaa aagatggcga ataataaaac
     8461 gttatttgat tgggtggaag accgcaaatc aacgctggaa gaaatagaac agacagattt
     8521 tttcgcgctg cctgaatttg tagaacgcaa tttaaaatat ccgttttttg aatggcaaaa
     8581 atcggcttta gaaaactttg tgatttttga ccgcgcttca aaattaaagg atttccctga
     8641 catcaaaaac cgccctaccc atttgctgtt caatatggca acaggtgctg gcaaaacgat
     8701 gatgatggcg gcgttgattt tgtattattt tgaaaaaggt tatcggcatt ttttgttttt
     8761 cgtcaatcaa aataatatcg tggataaaac ggaaaataat tttattgacc cggcgcacgc
     8821 aaaattttta tttaccgaga agattttgca aggtgatacg gtaattccta ttcgcaaagt
     8881 ggaaatgttc agcccacatt cagacggcat tgaaattaaa tttaccagca ttcaaaaact
     8941 gtataacgat atccacaccg agcgagaaaa ccaaaccaca ttggcagatt tgcacaaatt
     9001 gaaccttgtg atgctgggcg atgaagcaca ccatttaaac gcacaaacca aaggcaaaaa
     9061 acaagacgag ttggatttgg aagtggaatt aaaagccaat gcaggcaatg ccgaaattga
     9121 acgcaaaggc tgggagcata tggttttgga attgttgctc aataaaaatg gcaatcccgg
     9181 ccaaaatgtg ctgttggaat ttaccgccac gctgcctgaa aatgctgacg tgcaacaaaa
     9241 atatgcggac aaaatcatca caaaatttgg cttaaaagag tttttgcaaa aaggttatac
     9301 caaagaaatc aatttggtat ccagtacgct gaataagaaa gagcgggtgt tgcacgcttt
     9361 attatttgct tggtatcgcc atcaaatcgc gttgaaatac ggcattgcca atttcaagcc
     9421 tgtgatgttg tttagaagta agacgattga tgaatcaaaa gcggattatt cggcattttt
     9481 aaattgggca gaaaatgtac aagcggatga ttttgagttt ttagatacgt tttcaggcag
     9541 cctgaataat agcgacaacg ccaacgaaca aggcaaaacc cgaaccgaac aagccctaaa
     9601 atttatgcag gaaaatggct ttgagtttgc acatttggca aattgggtga aacaaaatta
     9661 tcaaaaacat aatgtgatta ttaccaactc cgaaaccaac aaaaacaaaa ctgaaaaaac
     9721 cgacagcgaa accgaaaaat tgctaaacaa tttggaagcg gctgataatc caatccgtgc
     9781 catttttacg gtagatagat taaccgaagg ttgggacgtt ctgaatttgt ttgatattgt
     9841 gcgtttgtat gaagggcaaa atggcggtgg ttcaaataaa aaatcaggca aaacggctgc
     9901 cgctaccgta tcggaaaagc agttgattgg tcgtggcgtg cgttattttc catttgcgtt
     9961 tgaaggtaaa cagccgaata aacgcaaatt tgacaacgac atgcaacacg aattgcgtat
    10021 cttggaagaa ttgttttatt acacgcacga tgagcaatcc cgctatattt cagaactgaa
    10081 aaatgagttg cgaaaagacg gttatttgcc tgaaaaagac gatgataagg tgttggcaac
    10141 atttaaactc aaatctgaat ttgccgataa taaggatttt agagagttgt taatttgggc
    10201 gaataaaaaa atccccaacc caaacgccaa aagcaataat gcagacagcc tgcaagccaa
    10261 tccgcaaacg ctttcattcc aaattcacgg caatcaattg ttgcaggaaa cgcaatttac
    10321 agccgatgaa aatgatgaaa cagcccgaca aatcggcaca caaaataatt ttactcaaac
    10381 cataaaaatg agtgaaatgg agcggcacat tttcaataaa gcattacata tcaaagggaa
    10441 aaatagtcaa tctttattcc attttgaccg cctgcaaagc aaactcaaca ttcaaaatcg
    10501 caatgaattg caaaataact tgttgaaaga ctggcaaatt gaatttttag gattagggca
    10561 agacaaacag gttcgcccgg atgataaact tgcaggctgc ctgaaaatct tggaaatggt
    10621 tgaaaaacat ctgaatgaaa gtgatatgcc gtttatcggt acaaaagaat ttacacctaa
    10681 aaaattatgg gaaattttcg gcacgccaaa acaaaaatgg gtcaaaaaag atgatgtaaa
    10741 aacgactatt gccacgcaaa atgattggta tgtgatggat aattttgctg gaacgggttt
    10801 ggaagaagcg ttaattcaat ttatttcagc gcgtttgggc gatttgaaat ctaaatatga
    10861 cgttcattta atccgtaatg aagaagtgtt taaattgaat aactttgccg atggtgaagg
    10921 atttatgccg gactttattt tattgctgaa agataaacaa aaatcttctt ccaatggtgt
    10981 gaacgacttt ttgcattacc aaattttcat tgagcccaaa ggtgagcatt tagtggaaac
    11041 tgatggatgg aaagaagaat ttttagaagc catcactgtg gaatatggga tggataagat
    11101 cctgcaaaaa gatacaccgc attatcgttt gatcggttta ccgtttttta ctgatcatca
    11161 gaaaaatgga caatttacgg agtcattccc tttaggtgcg gcatcgcttg aaaaatagag
    11221 tagtgcattg caggcaaccc cgtttgacaa aacttccttt acaaaagggc gttttgtcag
    11281 atatttaatc aacacattat taaaatacag ccaaatttta atgccgtccg aaccctgtgt
    11341 tcagacggca tcgtattttt cagtatctaa accgtttccc taccccaatc tttacccctt
    11401 aaaatcgagg catcgacatc ttgaatatcg cggtgtcccg taaacgccat agatatatcc
    11461 atttctttat atagaatttc cagcgcacgg gttacgcctt cttcgccata cgcgcccaag
    11521 ccatacagga acgcccgacc tatcattgta cctttcgcgc ccaaagccca cgccttcaaa
    11581 atatcctgac cgctgcggat gccgctgtcc atccaaactt cgatgtcgct gcccactgcg
    11641 ctgacgatgt cgggcaaggc tttgatggca gacacggtat cgtcgagctg tcggccgccg
    11701 tggttggaaa caatcaatgc gtccgcgccg cttttcgctg ctttttccgc gtcttcgggt
    11761 tccataatgc ctttgataat cagcttgccg ccccacaaat ctttaatgcg cgccacatcg
    11821 tcccagctca ggcgcgggtc gaattgttcg gaagtccatg aagacagcga agacaaatcg
    11881 ccgacgttct tcgcgtgtcc gacgatattg cggaacgtgc ggcgttccgt gttcagcatt
    11941 ttcatgcacc attccggttt ggtcgccaga ttgattaaat tggcgatggt cggtttcggc
    12001 ggcgcggaca ggccgttttt gatgtctttg tggcgttgac ccaaaacctg caaatcggcg
    12061 gtcaatacca atgccgaaca tttggcatcc ttcgcgcgct taatcaggtt ttccataaac
    12121 tcgcggtcgc gcatcacata aagctgaaac caaaacggcg cgctggtgtt ctcggcaacg
    12181 tcttcaatcg agcagatgga catggtggac aacgtaaacg gaataccgaa cttctccgcc
    12241 gcccgcgccg ccaaaatttc gccgtctgcg tgtgccatgc cggtaaaacc cgtcggcgca
    12301 atcgccaccg gcattttcac atcctgcccg atcattttag tctcaaggct gcgtccctcc
    12361 atattgacca atactttttg acggaagcgg atgtctttga aatccgaagt attttcccga
    12421 taagtcgttt ccgtccacga acccgaatcg atgtaatcgt aaaacatacg cggcattttg
    12481 cgcttggcaa cgcggcgcaa gtcttcgatg caggtcattt tgctcaaatc acgtttcatt
    12541 tgtcgcccct gaatacctga ataactttat atgaaataga taatgtatat caatattgat
    12601 tataaggcaa atcatttcaa catttactgc acccgccgcc gctcccactt caagcggcat
    12661 aaggtttaga attcaaaaat aatatattaa aatcaaaata ttaaaaatca atcaatctgc
    12721 cgatttaaac agccaatcgc gcaatccgcc cccatacttg actgaaacac tcagatattg
    12781 gacaattccg cccactataa aaaaagccga cacgggcaac caccaccatg agactgacca
    12841 ccaaagggcg tttcgccgtt accgctatgc tggatttggc gatgaacgcg caaaccggcg
    12901 ccgtcaaact cagtgccatc agcgaacgcc aaaacatatc cctctcctat ctcgagcaat
    12961 tgttcggcaa actccgccgc gccggacttg ttgaaagcct gcgcgggccc ggcggcggct
    13021 acatcctcgc cgcaccggcg gcacgcatca acatcgccca aatcatcgcc gccgccgaag
    13081 accggctgga cgcgacccaa tgcggcagca aagccaactg ccaccacggc gcgccctgcc
    13141 tgacgcacga cctttgggag aatttaaaca aaaccatcaa cgactacctc ggcagcgtta
    13201 ccctgcaaag catcatcgaa cagaaaaaca acggcgacgg cagccgcgtc gtccaattta
    13261 cacacatcca ttaaataaca cccgaaaaag aaagggcaaa ccatgaccgt caaaaccccc
    13321 gtttacctcg actacgccgc caccaccccc gttgacaaac gcgttgccga aaaaatgatt
    13381 ccctatctga ccgaaacctt cggcaacccc gcctccaaca gccacgcatt cggctgggaa
    13441 gcagaagagg ccgtcgaaaa agcccgcgcc gacatcgctg ccctgattaa tgccgacccc
    13501 aaagaaatcg tcttcaccag cggcgcgacc gagtccgaca acctcgccat caaaggcgcg
    13561 gcaaacttct acaaaaccaa aggcaaacac ctcatcaccg tcaaaaccga acacaaagcc
    13621 gttttagata caatgcgcga actcgaacgc caaggttttg aagtaaccta cctcggcgtt
    13681 caagaaaacg gtttgattga tttggaagaa ctcaaagccg ccatccgcga cgacaccatc
    13741 ctgatttccg taatgtgggc aaacaacgaa atcggcgtgg tgcaagacat tcccgccatc
    13801 ggcgaaatct gccgcgaacg taaaatcgtc ttccacgtcg atgccgccca agcctgcggc
    13861 aaagtgcctg tcgatgtcga agccgccaaa atcgacctgc tctcgatgtc cgcgcacaaa
    13921 gtgtacggcc ccaaaggcat cggcgcgctg tacgtccgcc gcaaaccgcg cgtccgcctc
    13981 gaagcccaaa tgcacggtgg cggccacgaa cgcggcttcc gcagcggcac attaccgacc
    14041 caccaaatcg tcggtatggg cgaagccttc cgcatcgcca aagaagaact cgaacaagat
    14101 atggcgcact accgcaaact gcgcgacatc ttcctcaaag gcatcgaagg catcgaagaa
    14161 gtctatatca acggcgacct cgaacaccgc gccccgaaca acctgaacgt cagcttcaac
    14221 ttcgtcgaag gcgaaagcct gattatggca gtgaaagaac tcgccgtatc cagcggctcc
    14281 gcctgcacct ccgcctcgct cgaacccagc tacgtcctgc gcgcattggg cagaaatgac
    14341 gaattggcgc actcgtccct gcgcatcacc ttcggtcgca tgaccactga agaagaagtg
    14401 caatttgccg ccgaactgat taaatccaaa atcggcaaac tgcgcgaact gtcgccgttg
    14461 tgggaaatgt tcaaagacgg gattgatttg aactcgattg aatgggcggc gcattaaagc
    14521 gtaccaacat gccatccgaa ccttcagacg gcattccaaa aacaaagcaa tcaagagaaa
    14581 aatatgaacg aacaagattt agatttggac aacctcgaca acctgcttga cgatttcgac
    14641 ggcgttaccg tggaaggcgg cgtcgattcg gaaaacgacg acggctgtga aggcggagcg
    14701 tgcaaaatct aactcaggcc gtctgaaaac cggcaaacaa accacttaaa tcatcaaaac
    14761 attaaggaaa ccacatcatg gcatacagcg ataaagtaat cgaccactac gaaaatcccc
    14821 gcaacgtcgg tactttcgac aaaaacgacg aatctgtcgg caccggcatg gtcggcgcac
    14881 cggcctgcgg cgacgtgatg cgcctgcaaa tcaaagtgaa cgatgaaggc atcatcgaag
    14941 atgcgaaatt caaaacttac ggctgcggtt ccgccatcgc ttcgtccagc ctgattaccg
    15001 agtgggtcaa aggcaaaagt ctggatgacg cgctggcaat caaaaacagc gaaatcgccg
    15061 aagagttgga attgccgccg gtaaaaatcc actgctccat cttggctgaa gatgcggtaa
    15121 aagcggccgt tgccgactac cgcaaacgtc aggaaaacag ataaagcctt tcagacggca
    15181 tcgtcccgca atgccgtccg aaccacccgc cgcttcaggt ccgtccgggg cggtacaaca
    15241 aggaagaaat atgattaccc ttaccgagaa tgccgcaaaa cacatcaatg actatctcgc
    15301 caaacgcggc aaaggcttgg gcgtacgctt gggtgtgaaa accagcggct gctcggggat
    15361 ggcgtacaac cttgaatttg tcgatgaagc cgatggcgac gacctgattt tcgaaggaca
    15421 cggcgcgcgc atttatatcg atccgaaaag cctggtttat ctggacggca cgcaagtcga
    15481 ttacaccaaa gaaggtttgc aggaaggttt caaatttgaa aaccccaatg tcaaagactc
    15541 ctgcggctgc ggcgaaagct tccacgttta aggcataaaa acggcgggac agtatcaaaa
    15601 ccgtcccgcc attttttcgc ttcctgcctg ttgtagctgc ctttgccttt ccttttccgt
    15661 tccaccttgt gccggaacaa atcggatttc actaaggctt ttaaagcatt gtcgcgtatt
    15721 ttgcctttat tgtgctgcac tttgccgccc atattcagtc ctttcgttta agaagctgca
    15781 gattataagg caaaaacagt tttctgccaa aatcttacat ttatcattct actatgtccc
    15841 aatatttcac cctcttccgg attgaacccg ctttcgatat cgacaccgaa aacttggaac
    15901 aaacctaccg cgccttggcc gcccgtttcc atcccgataa attcgcttca gcttccgcct
    15961 ttgagcaaaa gcaggcagtg atgatgtctt ccaccatcaa cgatgcctac cgcaccttga
    16021 aaaaccccat cgaccgcgcc gcctacctgc tgaaaacatc gggcatcgat gccgacgcgc
    16081 cggagcatac cgctttcgcc cccgaattcc ttatgcagca aatggaatgg cgcgaaacgc
    16141 tgatggaggc acgggcaggc aacgaccttg aatccttgaa aaatctcgac aacgaaatcc
    16201 gcgacgaaca agaaaaactg ttctgcggtc tgaaacagtc gtttgcccga caagattacg
    16261 acacagccgc acaacaagtc cgccaaggca ggtttctcga caaactccgc aacgaaattt
    16321 cctcggcatt ataatccgca ccgtgtttca gacggcgtaa ccgccgcacc gttccgcgtc
    16381 aaaatatgct aaaataagca acaatttttt gccatacgaa acattgaaac catgaccgac
    16441 gcaaccatcc gccacgacca caaattcgcc ctcgaaaccc tgccggtaag ccttgaagac
    16501 gaaatgcgca aaagctatct cgactacgcc atgagcgtca ttgtcgggcg cgcgctgccg
    16561 gacgttcgcg acggtctcaa gccggtacac cgccgcgtac tgtacgcgat gcacgaattg
    16621 aaaaacaact ggaatgccgc ctacaaaaaa tcggcgcgca ttgtcggcga cgtcatcggt
    16681 aaataccacc cccacggcga taccgccgta tacgacacca tcgtccgtat ggcgcaaaat
    16741 ttcgctatgc gttatgtgct gatagacgga cagggcaact tcggatcggt ggacgggctt
    16801 gccgccgcag ccatgcgcta caccgaaatc cgcatggcga aaatttccca cgaaatgctg
    16861 gcagacattg aggaagaaac cgtcaatttc ggcccgaact acgacggtag cgaacacgag
    16921 ccgcttgtac tgccgacccg tttccccaca ctgctcgtca acggctcgtc cggcatcgcc
    16981 gtcggcatgg cgaccaatat cccgccgcac aacctttccg ataccgtcaa tgcctgcctg
    17041 cgcctgctcg atgcacccga caccgaaatc gacgaactga tcgacattat ccaagccccc
    17101 gacttcccga ccggggcaac catctacggc ttgagcggcg tgcgcgaagg ctataaaaca
    17161 ggccgcggcc gcgtcgttat gcgcggtaag acccatatcg aacccatagg cagaaacggc
    17221 gaacgcgaag ccatcgttat cgacgaaatc ccctatcagg tcaacaaagc caagctggtc
    17281 gagaaaatcg gcgatttggt tcgggaaaaa acactggaag gcatttccga gctccgcgac
    17341 gaatccgaca aatccggtat gcgcgtcgtt atcgagctga aacgcaacga aaatgccgaa
    17401 gtcgtcttaa accaactcta caaactgact ccgctgcaag acagtttcgg catcaatatg
    17461 gtggttttgg tcgacggaca accgcgcctg ttgaacctga aacagattct ctccgaattc
    17521 ctgcgccacc gccgcgaagt cgttacccga cgtacgcttt tccggctgaa gaaggcacgc
    17581 catgaagggc atattgccga aggcaaagcg gtcgcactgt ccaatatcga tgaaatcatc
    17641 aagctcatca aagaatcgcc caacgcagcc gaggccaaag acaaactgct tgcgcaccct
    17701 tggcgcagca gcctcgttga agaaatgctg acgcgttccg gtctggattt ggaaatgatg
    17761 cgtccggaag gattggctgc aaacatcggc ttgaaagagc aaggttatta cctgagcgag
    17821 attcaggcag atgctatttt acgcatgagc ctgcgaaacc tgaccggcct cgatcaagaa
    17881 gaaattgtcg aaagctacaa aaacctgatg ggtaaaatca tcgactttgt ggatatcctc
    17941 tccaaacccg aacgcattac ccaaatcatc cgcgacgaac tggaagaaat caaaaccaac
    18001 tatggcgacg aacgccgcag cgaaatcaac ccgttcggtg gcgacattgc cgatgaagac
    18061 ctgattccgc aacgcgaaat ggtcgttacc ctgacacatg gcggctatat caaaacccag
    18121 ccgaccaccg actatcaggc gcagcgtcgc ggcgggcgcg gcaaacaggc ggctgccacc
    18181 aaagacgaag actttatcga aaccctgttt gttgccaaca cgcatgatta tttgatgtgc
    18241 tttaccaatt tgggcaagtg tcattggatt aaggtttaca aactgcccga aggcggacgc
    18301 aacagccgcg gccgtccgat taacaacgtc atccagttgg aagaaggcga aaaagtcagc
    18361 gcgattctgg cagtacgcga gttccccgaa gaccaatacg tcttcttcgc caccgcgcag
    18421 ggaatggtga aaaaagtcca actttccgcc tttaaaaacg tccgcgccca aggcattaaa
    18481 gccatcgcgc tcaaagaagg cgactacctc gtcggcgctg cgcaaacagg cggtgcggac
    18541 gacatcatgc tgttctccaa cttaggtaaa gccatccgct tcaacgaata ctgggaaaaa
    18601 tccggcaacg acgaagcgga agatgccgac atcgaaaccg aaatttcaga cggcatcgaa
    18661 gatgaaaccg ccgacagcga aaacgcactg ccgagcggca aacacggtgt tcgcccgtcc
    18721 ggtcgcggca gcggcggttt gcgcggtatg cgcctgcctg ccgacggcaa aatcgtcagc
    18781 ctgattacct tcgcccctga aaccgaagaa agcggtttgc aagttttaac cgccaccgcc
    18841 aacggatacg gaaaacgcac cccgattgcc gattacagcc gcaaaaacaa aggcgggcaa
    18901 ggcaatattg ccattaacac cggcgagcga aacggcgatt tggtcgccgc aaccttggtc
    18961 ggcgaaaccg acgatttgat gctgattacc agcggcggcg tacttatccg caccaaagtc
    19021 gaacaaatcc gcgaaaccgg ccgcgccgca gcaggcgtga aactgattaa cttggacgaa
    19081 ggcgaaacct tggtatcgct ggaacgtgtt gccgaagacg aatccgaact ctccgacgct
    19141 tctgtaattt ccaatgtaac cgaaccggaa gtcgagaact gaaaatcatc tcccgatgcc
    19201 gtctgaagat tcagacggca tttattttat ccctcatccg tcatccagct tctcacaata
    19261 tagcggatta tagtcaatta aaaacaaggg gctgtcctag ataactaggg aaattcaaat
    19321 taagttagag ttgcccctat gagaaaaagt cgtctaagcc ggtataaaca aataaactca
    19381 ttgaactgtt tgtcgcaggt gtaactgcaa gaacgacagc agagttagta ggcgttaata
    19441 aaagtaccgc agcctattat tttcatcgtt tacgattact tatttatcaa aacagtccgc
    19501 atttggaaat gtttgatggc gaagtagaag cagatgaaag ttattttgct gaacgacaaa
    19561 accatatcaa tggaattgag aacttttgga accgggcaaa acgtcattt
//
LOCUS       DAWFAD010000025        19569 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWFAD010000025 DAWFAD010000000
VERSION     DAWFAD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19569
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.025"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(65..1312)
                     /gene="fabF"
                     /locus_tag="WGO49_000652"
     CDS             complement(65..1312)
                     /gene="fabF"
                     /locus_tag="WGO49_000652"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ0305788.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="WGO49_000653"
     CDS             complement(1468..1704)
                     /gene="acpP"
                     /locus_tag="WGO49_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ0305789.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            1936..2943
                     /locus_tag="WGO49_000654"
     CDS             1936..2943
                     /locus_tag="WGO49_000654"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ0305790.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     Q"
     gene            2959..3138
                     /locus_tag="WGO49_000655"
     CDS             2959..3138
                     /locus_tag="WGO49_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305791.1"
                     /translation="MPSERTFCRSDGIFISFSCLPPFENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            3277..3600
                     /locus_tag="WGO49_000656"
     CDS             3277..3600
                     /locus_tag="WGO49_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305792.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            3687..3968
                     /locus_tag="WGO49_000657"
     CDS             3687..3968
                     /locus_tag="WGO49_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305793.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            4021..4494
                     /locus_tag="WGO49_000658"
     CDS             4021..4494
                     /locus_tag="WGO49_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ0305794.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            4748..5083
                     /locus_tag="WGO49_000659"
     CDS             4748..5083
                     /locus_tag="WGO49_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305795.1"
                     /translation="MTVDKEQVEKMIYSENKQSVIDGMLGMTFSSEEDEIPWISEKLT
                     ELSKHKDLDIARLSLTCFGHLARMHENIGDCDKVIALLLSKQGDPDFQGFAEDALDEI
                     SLFIFKKRP"
     gene            5263..7953
                     /gene="glnE"
                     /locus_tag="WGO49_000660"
     CDS             5263..7953
                     /gene="glnE"
                     /locus_tag="WGO49_000660"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ0305796.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRDTKSPQHLSVVAMGKAGGYELNVSSDIDLIFIYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVLTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVGKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLEKLAESGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQRQRLAESMGFDDYADFLNAL
                     NQHRNQVNRLFNEILSEPGEQTQSSGEWQWVWQEKPDEEGRQCRLKEHGFDAETIAAR
                     LDQIRNGHKYRRLSAHAQPRFDALVPLLVQAAAAQTNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWDKLAAELS
                     DGIKACGGDTEAQMDTLRHFQHAQVFRLTVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAIVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALSGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLANYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAEKTEVSGELLAHYGNVRKLWREVFGEEAATA"
     gene            complement(8253..8945)
                     /locus_tag="WGO49_000661"
     CDS             complement(8253..8945)
                     /locus_tag="WGO49_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0305797.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGTPEYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(9053..10246)
                     /locus_tag="WGO49_000662"
     CDS             complement(9053..10246)
                     /locus_tag="WGO49_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0305798.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(10375..11112)
                     /gene="pxpA"
                     /locus_tag="WGO49_000663"
     CDS             complement(10375..11112)
                     /gene="pxpA"
                     /locus_tag="WGO49_000663"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ0305799.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGQVKAVDGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(11099..12028)
                     /locus_tag="WGO49_000664"
     CDS             complement(11099..12028)
                     /locus_tag="WGO49_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ0305800.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(12025..12672)
                     /gene="pxpB"
                     /locus_tag="WGO49_000665"
     CDS             complement(12025..12672)
                     /gene="pxpB"
                     /locus_tag="WGO49_000665"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ0305801.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            12856..15063
                     /gene="uvrD"
                     /locus_tag="WGO49_000666"
     CDS             12856..15063
                     /gene="uvrD"
                     /locus_tag="WGO49_000666"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ0305802.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPVDVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FILDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKIAAFVRLIEALRNQVGQLPLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFEILPENISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSIKKTAYDSYGNTRQTATFKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            15298..15837
                     /locus_tag="WGO49_000667"
     CDS             15298..15837
                     /locus_tag="WGO49_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0305803.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYKEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAKDHVQICEVSGIGFEKEGSLCGETGQK
                     LWMEEWEYPPIGVYFANDPSGGHAMFALDYRECGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(16027..16242)
                     /locus_tag="WGO49_000668"
     CDS             complement(16027..16242)
                     /locus_tag="WGO49_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305804.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(16250..16453)
                     /locus_tag="WGO49_000669"
     CDS             complement(16250..16453)
                     /locus_tag="WGO49_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305805.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(16366..16725)
                     /locus_tag="WGO49_000670"
     CDS             complement(16366..16725)
                     /locus_tag="WGO49_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0305806.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            16778..17155
                     /locus_tag="WGO49_000671"
     CDS             16778..17155
                     /locus_tag="WGO49_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0305807.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNRAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVQISILKQLVKSSLS"
     gene            17354..17974
                     /locus_tag="WGO49_000672"
     CDS             17354..17974
                     /locus_tag="WGO49_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0305808.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            17997..19448
                     /locus_tag="WGO49_000673"
     CDS             17997..19448
                     /locus_tag="WGO49_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEZ0305809.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLNPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
BASE COUNT         4820 a         5099 c         4952 g         4698 t
ORIGIN      
        1 ctataaaaat gccgtctgaa acggtttcag acggcatttc gatgtcggcg gcggctttgc
       61 ggaatcagcc tttgaagcgt ttgaagacca gcgtgccgtt ggtgccgccg aagccgaagg
      121 agttggaaat ggcaacgtcg atttccgcgt cgcgcgcttc gttggcgcag tagtccaaat
      181 cgcagccggc ttcaacgtct tgttcaaaaa tgttgatggt cggcgggatt ttgccgtcgt
      241 gtatcgccaa aatgctgtac acggcctcca cgccgcccgc cgcgccgagc aggtggccgg
      301 tcatggattt ggtcgagctg acgacggttt tgtaggcatg ttcgccgaac gcgcgtttga
      361 gggctttggt ttcgttggca tcgcccaagg gggtggacgt gccgtgcgcg ttgacgtaat
      421 ccacgtcttc gggattgatg ccggcatctt tcagcgcgcg ggtaacggca agggcggggc
      481 cttcttcgtt cggcgcggtg atatggtaag catcggaact catgccgaag ccgacgattt
      541 cggcgtagat tttcgcgccg cgttttttgg cgtgttccaa ttcttccaac accaatatgc
      601 ccgcgccttc gccgataacg aagccgtcgc ggcctttgtc ccacggacgg gaagcggtgg
      661 cggggtcgtc gttgcgggtg gagagggctt tcatcgcggc aaaaccgccc acgcccaaag
      721 tgctgattgc gccttccgcg ccgccggcaa ccattatgtc cgcgtcgccg tatttgatca
      781 gtcgggcgga attgccgatg gcgtgcgcgc cggtggtaca ggcggaaacc atcccgtagc
      841 tcgggccgcg gtagcctttg aggatggtaa cgtgtccgga aatcaggttg atcagcgaac
      901 cggggataaa gaaagggttg attttgcgcg cgccgccttc gattacggct ttgccggtga
      961 cctcgatgct gggcagtccg ccgatgccgg aaccgatgtt cacgccgatg cggtctttgt
     1021 cgaggttttc cacatcgtcc aagcccgaat cggcgattgc ctgcaatgcg gcggcaatgc
     1081 cgtagtggat gaatacgtcc atccggcgcg cttctttcgc gctgatgtat tgtccgatgt
     1141 cgaaaccgcg cacttcgccg gcgacacggc tgttgatgtc ggatgcgtca aagcgggtaa
     1201 tcgcgccgat gccgcttttg ccggcgagca gggtgtccca agcctctgcg acagtgttgc
     1261 cgacagggga aacctgacct aagcctgtaa tgactactct tctctgactc atgataacct
     1321 cgctgttggt tgtcggaatg ggggcatatg cggctgtcgt gcagatgccg tctgtaattt
     1381 gcggcagggg ttcaaacagt ttgccatata agggaaaagc ctctattgcg cggtgcagca
     1441 gaggctgttg tgtcgggcga cgaccggtta gccgttgtgg gcattgatgt agtcgatagc
     1501 cagttggacg gtggtgattt tttcggcatc ttcgtcgggg atttcgcagc cgaatgcttc
     1561 ttccaaagcc ataaccagct ccacggtgtc caaagaatcc gcgcccaagt cgtcttggaa
     1621 agaagattcg tttttcacgt cggcttcgtt tacgcccagt tgttcagcaa caattttttt
     1681 aacttgttgt tcgatgtttg acatatcagt cgttccttta tgccttgcgg caggttgttt
     1741 aagggaaata aatcggtggt attgtaccga cttttaatag agttttctat ctaatgacta
     1801 ttatatcaat atttgccgat ttgtacattt ttgggtgcgg cgggttttgt cgttcaagtt
     1861 tgacctgtgt gccgtatgtt tgacgggatt tcggttaaaa tgacggcatt tccatctgaa
     1921 gcagaaagcc ctgtcatgta tccacttgcc cgccgcatcc tgtttgcact cgatgccgag
     1981 aaagcccacc acttcacgct cgacgcgctc aacatggttt ataaattggg tctgattccc
     2041 gtaaccggca accgtaccaa acctgtaaaa ctgatgggtt tggatttgcc caaccctgtc
     2101 gggcttgccg ccggactcga taaaaacggc gaatatatag acgcattggg cgcgctcggc
     2161 ttcggtttca tcgaaatcgg cacggtaacg cccaacccgc agcccggcaa cccgcagccg
     2221 cgcctcttcc gcgttcccga atatcaaggc atcatcaacc gcatgggctt caacaaccac
     2281 ggcatcgatg ccatgattca aaacatcgaa aaaagtaaat tcagcggcgt attgggcatc
     2341 aacatcggca aaaacgctgt aacaccgatt gaaaacgctg ccgatgatta tttaatctgc
     2401 ctcgaaaaag cctacgcaca cgcaagttac attaccgtca atatttcctc gcccaacact
     2461 aaaaacctcc gcgcgctgca aggtggcgac gagttgagcg cattgcttga ggctttgaaa
     2521 aacaaacagg cacagcttgc ctctgtacac gggaaatacg tcccgctcgc cgtcaaaatc
     2581 gcccccgatt tggatgaagc acaaatcgaa gacatcgccc acgttgtcaa atccgtcgaa
     2641 atggacggca tcatcgctac caataccacc atcgacaaat caagtctcgg cagccatccg
     2701 ctcgcaggcg agcagggcgg tttgagcggg ctgcccgttc atgaaaaaag taatcgggtg
     2761 ttgaagctgt tggcagacca catcgacggc aagctgccga ttatcggcgt aggcggcatt
     2821 atggaaggca gggacgcggc agagaaaatc cgcttgggcg caactgccgt ccaggtgtac
     2881 agcggattga tatacaaagg tccggcattg gtcaaagaat gtttgaaggc tttggcgcaa
     2941 tgacgcgtcc cgcacaaaat gccgtctgaa cgcacgtttt gccgttcaga cggcattttt
     3001 atttcctttt cctgcctgcc gccctttgaa aatcccttac gcgccgccct gtttgaaaca
     3061 aagcaaacgc ccgccgcgaa ccggacaatc ttgatacgca gccaaagcaa gtccggcggt
     3121 aagttgttaa acatataaat aattgatatg aaaaaataat tttatgccgt ggtggacggt
     3181 ttgagaaaac gcaaaatatg aacggcgttg aaattcatga tgtggaaaat aaggatacgg
     3241 cggataatca aaagatttta tatttaagga tttgatatgt ttaaggacga actaaatgaa
     3301 tttatccgat tgatatccga tccggaaagt gaattggatg aatggtattt gagtgatttt
     3361 aaagatgaac atatatggga aatgcaaagt tacgaagcat tttcatgttt gcgggaagca
     3421 gtcccctatt tgtttgctta tccccgatac gggtatgaac tgttggagat aatttccgcg
     3481 ttaaaagaaa catctgatac gacagaatta ttttatgagc cgggtatcgt gcctttgctg
     3541 attgatttat ataaagaaga tagttatctg gtaaatatgg tcaaacggat attcaaatga
     3601 tgaaaggggc gcgggcgttt gcttggcgga aggcaggtaa ctcaatttga aggacgcgga
     3661 agccgatacg ttaaaaagga tgaaaaatgg aaaacgggca acggaataac cggtttcctt
     3721 tggaaaaacg aattttttat ctggagcatt cggggcggta tctgatgatt tgcgccttat
     3781 cggattattc ccaaaacaaa catactgtcg ttatggcaaa tttcatctat ccggatgaaa
     3841 aaacggattg gcggaatttg gatgatttat tcaatgagtt ggttttagag gaattgcagg
     3901 cttcatttat ggattggcat ccgactgttg aagaggcaat cagccgtcat ttggaagact
     3961 tttcgtaaca ggcgtcaatc ggaaggggcg gaatgacggg gcaggcaatc ggagtaaaaa
     4021 atgaaccttg atttaaccgc gcaaaaagtc cgtctttctt ggaaggatat tctgtggggg
     4081 tatgggaata aatacttggg ttgggctgat gtggcagctt atgcccgaaa aatgacgctt
     4141 tcagatcatg atgaacgtgt gttcaaacta tctttaatca acaaatccaa tattcttgaa
     4201 ttaaagcctg ttctggaaga tttggcttcg gaaatgaggg attattcccc taaaaattgg
     4261 ctgtacgtcc tcttaagcga tgtattccat agaaaagaag aatttgagga tcctttgggg
     4321 gaagttgaaa aaatttatgc agattttgat tatccggaag aaatagaatc atttgtcagg
     4381 tatatgccgc ccaaagacgg ttatattcct tctgcccaca cctatgaaga aaatattgcc
     4441 cggttatatt ctcactggga acactatttg aacaacggcg gagggcaggg ttaaaaccgg
     4501 caatccgatg ccgtctgaag cattatccgg ccttcagacg gcaaacggat tagagcggaa
     4561 gatttgaaag cgggggcggg ctgcattccg gaagcggcaa aacccgaacc gcccgcatcg
     4621 ttgccgataa accgataagg aaatcgtttt gccggaccgg gtgcaaatgt ttgataacaa
     4681 tcctgccgcc cggcaaaaat gctttaaaga aagcaggatt ggttaattta aaaggaaaaa
     4741 ttaaaaaatg acggttgata aggaacaagt agaaaagatg atttattctg aaaataaaca
     4801 gagtgttatt gacggtatgc tcggtatgac cttcagctcg gaggaggatg aaatcccctg
     4861 gatttccgag aagttgacgg agttgtcgaa acataaagac ttggatattg ccagattgtc
     4921 attaacctgt ttcggacatc tcgccaggat gcatgaaaat atcggcgatt gcgataaagt
     4981 gattgcttta ttgctctcaa aacaaggtga tccggatttt caaggttttg ccgaagatgc
     5041 gttagacgag atttctttat ttatatttaa aaagcgtccg taagcgcgga ttaaacctgt
     5101 caatccgatg ccgtctgaag cgttatccgg ctttcagacg acatcgaagg ttgctatgat
     5161 atagtggctt gactttaaac cagtacggca tcccctcgcc ctgtcctgat ttgaagtgaa
     5221 tccgccatca tcttcctgcc attttttcaa acggaatccg aaatgtccga caaccgcctc
     5281 gacactgccc gccgccattc cctgttcctt gcccgccagc tcgacaacgg caaactcaag
     5341 cctgaaattt tcctgcccat gttggacaag gttttgaccg aagcggattt ccaagccttt
     5401 gccgactggg acaaaatccg tgcggaagaa aacgaggaag aattggcgcg gcagttgcgc
     5461 gggttgcgcc gttatgtggt gtcgcagatt atcgtgcgcg atatcaaccg catcagcgat
     5521 ttgaacgaag taacccgcac gattacgctg tttgccgatt ttgccgtcaa taccgcgctg
     5581 gattttgcct acgcctatta tcgggatatg tacggcacgc cgatcgggcg tgatacgaaa
     5641 tcgccgcagc atttgagtgt ggtggcgatg ggcaaggcgg gcggctatga gttgaacgtg
     5701 tcttccgaca tcgatttgat tttcatctat cccgaatcgg gcgataccga cggtaggcgc
     5761 gaacgcggca atcaggaatt tttcaccaaa gtcgggcaga aactgattgc gttgctgaat
     5821 gacattaccg ccgacgggca ggtgttccgc gtcgatatgc ggctgcggcc ggacggcgat
     5881 tcgggcgcgc tggtattgag cgaaaccgcg ctggagcaat acctgattac gcaggggcgc
     5941 gaatgggaac gttatgcgtg gtgcaaaggc cgcgtgctta cgccgtatcc gaacggcatc
     6001 aaatcgctgg tgcgcccctt cgtgttccga aaatatctgg attacggcgc gtatgaggcg
     6061 atgcgtaagc tgcaccgcca aatccgcagc gaagtcggca aaaaagggat ggcggacaac
     6121 atcaaactcg gcgcgggcgg catccgcgaa gtcgaattta tcgcccagat tttccagatg
     6181 atacgcggcg gacaaatgcg cgcgctgcaa ctgaaaggca cgcaggaaac gctggaaaaa
     6241 ctcgccgaat cgggcattat gccgtctgaa aacgtcgaaa ccctgctcgc cgcctaccgc
     6301 tttttgcgcg atgtcgaaca tcgtttgcaa tattgggacg accggcaaac ccaaaccctg
     6361 ccggcttcgc ccgaacagcg gcaacggctt gccgaaagta tgggatttga tgattacgcc
     6421 gattttttga acgctttaaa tcaacatcga aaccaagtca accggttgtt caacgaaatt
     6481 ttgagcgaac ccggagaaca aacgcaaagc agcggcgaat ggcagtgggt ttggcaggag
     6541 aagcccgatg aagaagggcg gcaatgccgt ctgaaggaac acgggttcga tgccgaaacc
     6601 atcgccgcgc ggctcgacca aatccgcaac gggcataaat accgccgcct ttccgcccac
     6661 gcccagccgc gtttcgacgc gcttgtgccg ttgctggtac aggcggcggc agcgcaaacc
     6721 aacccgaccg atacattgat gcggctgttg gatttcctcg aaaatatcag ccgccgttcc
     6781 gcctatctcg ccttcctcaa cgagcatccg caaaccctgg cgcaactcgc tcagattatg
     6841 agccaaagct catgggtggc ggcgtatctg aacaaatatc cgattttgtt ggacgaactc
     6901 atcagtgcgc aattattaga tacggcgttt gattgggaca aactcgccgc cgaactttca
     6961 gacggcatca aagcctgcgg cggcgacacc gaagcgcaaa tggacaccct gcgccatttc
     7021 caacacgccc aagtcttccg cctcaccgtc caagacctcg ccggattgtg gacggtagaa
     7081 tccctctccg accaactctc cgcccttgcc gacaccgtca ttgccgccgc cctttcgtgc
     7141 gcgtgggcgg atatgcccaa aaaacaccgc gacacaccgc aattcgccat cgtcggctac
     7201 ggcaaactcg gcggcaaaga actcggctac gcctccgacc tcgacttggt ctatctttat
     7261 gacgacccgc atcccgatgc aggcgacgtg tacagccgcc ttgcccgccg cctgaccaac
     7321 tggctttccg ccgccaccgg cgcaggcagc ctctacgaaa ccgacctgcg cctgcgccct
     7381 aatggcgacg ccggtttcct cgcccacagc atcgccgcct ttgaaaaata ccagcgcgaa
     7441 aacgcctgga cgtgggaaca ccaatccctt acccgcgccc gcttcatctg cggcacaccc
     7501 gaaattcaga cggccttcga ccgcatccgc accgaaatcc tcaccgccga acgcgaccaa
     7561 accgccttgt caggcgaaat catcgaaatg cgcgaaaaaa tgttccccac ccatccgcct
     7621 gccgacagca acgtcaaata cgcacgcggc ggcgtggtcg acgtcgaatt tatcgtccaa
     7681 tacctcatcc ttgcccatgc ccgaaaatat ccgcaacttc tcgccaacta cggcaacatc
     7741 gccctcttga atatcgccgc cgactgcagc cttatcgaca aaaacctcgc cgaacaaagc
     7801 cgcaccgcct accgcttcta ccgccggcag caacacaaca ccaaactgcg cgacgcagaa
     7861 aaaaccgaag taagcggcga attgctggca cattacggca atgtcaggaa attgtggcgg
     7921 gaagtgttcg gcgaagaagc ggcaaccgcc tgaacaaaaa atgccgtctg aagcctgaca
     7981 atctgggttt cagacggtat tttcgtaccg tgccgtttta aggttgcggc agagctaaag
     8041 cgatttatcg ggaatggctg aaacccaaaa accggattcc tctttcagtt acagcagctt
     8101 gatccctttc ccttatccaa cgggggaagg ctaggatagg gtggcttgca aatatacaga
     8161 acaagggaca agagccaccc tctctccaac cctctccctc cgtacgggag ggggtggatt
     8221 ctcgcgggcg aagcccacgc tacggttagc ctttacccca gcacaaacaa ttcccgcccg
     8281 tgcgccttca gccaactttt agcattgtcg gtatgcggcg tcagcgtgtt caccaaatgc
     8341 caaaagcgcg gactgtggtc ggggtggcgg aggtggcaga gttcgtggat gcagacatag
     8401 tcggcgacgt attcgggcgt gccgatcagc cgccagttga ggcggatgcc ggtgtgcggg
     8461 cggcatacgc cccaaaaggt tttggcgttg ctcaggtctg tggcggtggg cgtcagtcct
     8521 gtttcggctg cgtgtttttc aaggcggggc agcaggtatt cgcgggcgcg ttcgttcaac
     8581 aggcggcgca ggtggtcgat ttgtgcggcg gtttcttttc ggggaagcag gatttcagac
     8641 gacgtgatac ggatatggct ttggctgtgg gtatccagct tggtctttat tccccgatac
     8701 caaatccact cgggtaagtt tgggtgggaa acaggatgca cgggcgtttt ggcaagcgtg
     8761 ttccgcaaaa tcgtttcgtt tgccgccagc cagtttgcta acgcgtggtc ttgaaaaaag
     8821 ggtgggacgt tgatgctgac cgtctgcata ttgacggggc gcagaatcag atttttcttg
     8881 gcactgcgtt tgagttcgat ttcgatgcac aaaccgtcgg aaagagtata ggtgaagcgt
     8941 ttcatagttg tgaataggtt tcagaccgga tacatcgtct gaaacaggaa ttttccatat
     9001 caggcggcaa acttcggata atatacaaaa tcaaacatct gcgctacaag gttcagccga
     9061 acaagccgcc gatatatttg ctgatggtga tggcgctgag tactgccatc aaaccgacca
     9121 caatcacgcc ggaaacggtg agccacagcg ggtgtttgta gtcgccgaca attttggttt
     9181 tgtaggcggc aatcagaatc agaccgaggg aaatcggtaa aatcaggccg tttaatgcgc
     9241 ctacgaacac cagcacctgc gccggtttgc cgatggtgga aaatacggcg gtggacacgg
     9301 cgataaaggc aataatccat ttgtttttat tgcgttcgat agacgggctg agaccggaga
     9361 agaacgacac cgaagtataa gccgcaccaa tcaccgaagt aatcgaagcc gcccaaatca
     9421 ccacgccgaa aatcagcagg ccgatgtatc ccgccgcata ttcaaacggt gtggaagcag
     9481 ggttgtcggg attgagctgt acgccttggc tgaccacgcc caaaaccgcc aaaaacaata
     9541 caatccgcat aatcgaggca atcaggatcg cccgcaccga gctttggctc acttccggca
     9601 acgccgattt gcctttgata cctgcgtcca gcagacggtg cgcaccggcg aaggtgatgt
     9661 agccgccgac cgtgccgccc accagtgtaa caatcgccat tgcatcgagt ttttccggca
     9721 taaaggtatg cacggcggca tctgccagcg gcggattcgc ctgccatgcc acataaaccg
     9781 tcagcgcaat cattacgaaa cccatcactt gggcgaattt gtccatcact ttgcctgctt
     9841 ctttaaacag aaacacaccg atggcaatca cgccgctgat cacggcaccg gtttccggtg
     9901 acagtccggt cagcaggttc agacccaagc ctgcgccgcc gacgttgcca atattgaacg
     9961 ccaaaccgcc catcacaatc agcacagcca agaaatagcc tgcgccgggc aagacctgat
    10021 tggcaatatc ctgcgcctgt ttttcggaaa cggcgacaat ccgccaaata ttgagctgcg
    10081 ccccgatgtc gagcagaatc gagagcagaa tcacaaagcc gaaacttgcc gccagtgctt
    10141 gggtgaaggt ggcggtttgg gtcagaaagc ccgggccgat ggcggaagtc gccatcagga
    10201 atgcagcgcc gattaaggca tttctgcggt ttttttgatc agacataatc gcttatcctc
    10261 tataaaattg gttgttgctg tgtttgggcg aaacctgcgg ttttagctac gcagaaactc
    10321 gctttgctcg ttttggcgaa acctgcggtt ttcagacggc ctatgaactg tttttcaagc
    10381 agaaactttg atgcctgccg ccagtagttc ctgccggatt ttttcggcaa acaccacggc
    10441 gtgcggcccg tctccgtgca gacagatgct gtcggcttgc acggcaacca ggctgccgtc
    10501 cactgctttg acctgcccgt cccgcaccat ctgcaatact tgggcgatgg cttcttcgtc
    10561 gctgtccacc tgcgcatcgg ggcggctgcg gggaaccagc gtaccgtcgg gcatatagcg
    10621 gcggtcggcg aatacttcgg aaatcacacc caagcctgcg gcttttccgg cttccaagag
    10681 caggctgccg gaaagtgcca tcaatttcaa tttcgggtcg aaatccgcca caattcgggc
    10741 aacggtatcc gccagcgcac ggtttttcgc cgcttgattg tacattgcgc cgtgcggttt
    10801 gacataagcc atttccaaac cctgatcacg gcacaaggcc tgcaatgcgc ccaactggta
    10861 attcagacac gcccgcaaat cggcttcgga cagattcatt tcggtacggc cgaagttttc
    10921 ccgatcggga tagccggggt gtgctccgat gcgcacgccg ttttgttggg catacgccaa
    10981 tgccgcccga atatcggcaa tgctgccggc gtgttgggcg caggcgatgt tggccgaagt
    11041 aatcagctgc aacaaggctt cgtcgctgcc gcagccttcg gcgagatcgg cgtttaaatc
    11101 aacctgcttc atgggtgatt ctccgtattt ggttcagata ggcttggttt ttgcgccgca
    11161 gggcggtggc ttctttcaag ccgattattt tgaatttgac tttgctgccg aagcgcacct
    11221 gtgccagcct gcccaaatcg gcggcggcaa cggtagcgat tttcggataa ccgccggtgg
    11281 tttgcgcatc ggccagcagg ataatcggtt tgccgccggg cggcacctgc acggttcctg
    11341 cctgaacagc gtgggacagc atttccaaag gttgcgacag ggtcagcggc tgtccgtcga
    11401 agcggtagcc catgcggttg ctatcgcttt gcagcgtcca cgtttcccgt tccagattca
    11461 gacgcccttt ttcactgaaa gcggcatatt ccgacgaagg aacaaggtgg acggtatcgg
    11521 taaacggtat cggggcaatg ccgactttgg acaattcctg cgcacctttg ccgatgggga
    11581 gataatcgcc tttttgcagc attctgccct gatggccgcc gaaaccggct ttcaggtcgg
    11641 tacttctcga acccatcact tccggcacat caaatccgcc cgccacgcac acatagccgt
    11701 acatgccctg cacggcacgc accattttca aggtctgccc tttgcgggcg gtataacgcc
    11761 aatacgaata gaccggttcg ccgtccaatt ccgcctgata cacggcaccg gtgagacaaa
    11821 acggcgtatc ccgttcaaac accagcatta tcccgcccaa agcgatttcg attgcggccg
    11881 tgccttcgtc gttgcccaat aaaatattac ccgccgccaa agcaaccgtg tccatcgcac
    11941 cggcatgacc gatgccgtaa cgccggtgtc cgtagcgtcc ggtatcctga atatgcgccg
    12001 gtgcctgcac tgccgaaacg tgaatcatgg ctcaatcctt tctgcaacaa agcggacttg
    12061 gtcacccgcc gccagcaggg tcggcggatt caaatcggct cggaacaagg gtaattcggt
    12121 tctgccgata atctgccagc cgccgggcga agcgaacgga tacacaccgg tctgactgcc
    12181 gccgataccg accgaaccgg caggaacgga cgttctcggc acggcacggc ggggcgtgtg
    12241 caatgcttcg ggcaagccgc ccagataagg gaaaccaggc tggaagccca tcataaatac
    12301 ggtataagtt tgcgccgtat ggcggcggac gatttcggaa ataaccgtct gatggaaagc
    12361 agcgacttcc gccaaatccg ggccgtattc gccgccgtag cagacgggaa tttccaccag
    12421 tttgccctga tggtctgtaa cggcggtgtg ttcccacaca tattgcaatt catcggcaag
    12481 cgtcgccaaa tcggtatcga aacgggtgaa cacggtcaga ttgttcatgc cgaccaccac
    12541 ttcctcaatc ctgtcgtgct gcccgagcgc agcggcaaac gcccacaact tttgctgttt
    12601 gcccagttcg gaaggtgcat tcagtcggca gaccaaagcg gattcgctga ttggtgtgat
    12661 ctctattctc atttgttgtt cattttggtt atgttttaat gaatctatat gcaggggcgg
    12721 cggtttgtca atatcttctg tgctgcatca tcaaaccgtc gattggaaaa gtgctgccct
    12781 gccgctgcac tttttcagac gaccttaaac cgtttctatt aaaatagcgc attccacttt
    12841 tcagacggca tctctatgtt tcccgaccaa tccgccccca acctgctgca aggcttgaac
    12901 cctgaacaac tttccgccgt aacctggccg ccgcaatccg cccttgtgct ggcaggcgcg
    12961 ggcagcggca aaacgcgcgt gctgaccacg cgtatcgcat ggcttttgca aaccggtcag
    13021 gcaagcgtgc acagcattat ggcggtaacg tttaccaaca aagccgccaa agaaatgcaa
    13081 acccgactcg gcgcgatgat tcccgtcgac gtccgcgcta tgtggctcgg cacgttccac
    13141 ggcctctgcc accgctttct acggctgcac caccgcgatg ccggtttgcc gtcttccttt
    13201 caaatcctcg acggcggcga ccagctttcc ctcatcaaac gcctgctcaa aagcctcaac
    13261 atcgccgaag aaatcatcgc gccgcgttcg ctgcaaggct ttatcaacgc gcaaaaagaa
    13321 tccggtttgc gcgcttccgt cttgagtgcg cccgatccgc acacaagccg catgattgaa
    13381 tgctacgccg aatacgacaa aatctgccaa cgcgaaggcg tggtcgattt tgccgaactc
    13441 atgctccgca gctacgaaat gctgcaaagc aacgaaatcc tgcgccagca ttaccaaaac
    13501 cgcttcaacc acattctcgt cgacgagttc caagacacca acaaactgca atacgcctgg
    13561 ctcaaactca tggcgggcgg aaacgcagcg gtatttgccg tcggcgacga cgaccaaagc
    13621 atttaccgtt tccgtggcgc aaacgtcggc aacatgaccg cgctgatgga agaattccac
    13681 atcgacgcgc ccgtcaaact cgaacaaaac taccgctccg tcggcaacat ccttgccgcc
    13741 gccaatgccg tgattgaaaa caacgacgaa cgactcggca aaaacctgcg caccgacgcc
    13801 gaagcaggcg acaaaatccg ctactactcc gcctttaccg acctcgaaga agcccggttc
    13861 atcttggacg aaaccaaagc cctcgaacgc gaaggctggg atttggacga aatcgccgtc
    13921 ctctaccgta gcaacgccca atcccgcgtt atcgaacaaa gcctgttccg cagcggcatc
    13981 ccctacaaaa tctacggcgg cttgcgcttt tacgaacgcc aagaaatcaa acacgcgctc
    14041 gcctacctac gcctcgccgt caatcccgac gacgacaacg cccttttgcg cgtcatcaac
    14101 ttcccaccgc gcggcatcgg cgcacgcacc gtcgagaacc ttcagacggc ctcaaacgaa
    14161 caaggcatca ccctttggca ggcagcctgc aacgccggcg cgaaagccgc caaaatcgcc
    14221 gccttcgtcc gcctgattga agcactgcgc aaccaagtcg gacaactgcc cctgtccgaa
    14281 atcatcgtcg gcatcctcaa agacagcggc ctgaccgaac actaccgcac ccaaaaaggc
    14341 gacaaccaag accgtctcga caaccttgac gaactcgtca atgccgccat cgagttcaaa
    14401 cccgaagaca gcaacttcga aatcctgcct gaaaacattt cagacgaccc agccttcccc
    14461 atcctcgcct tcctaagcaa tgccgccctc gaatccggcg aaaaccaagc aggcgcgggc
    14521 gaaaaagccg tccaactcat gaccgtccac gccgccaaag gcttggaatt taacgccgtc
    14581 ttcctcaccg gcatggaaga aggccgtttc cccagcgaaa tgagccttgc cgagcgcggc
    14641 ggcctcgaag aagaacgccg cctcatgtac gtcgccatca cccgcgcccg caaacgcctc
    14701 tacatcacca tggcgcaaca aagaatgctg cacgggcaaa cccaattcgg catcgtctcc
    14761 cgcttcgtcg aagaaatccc gcccgaagta ttgcactacc tgtccatcaa aaaaaccgcc
    14821 tacgacagct atggcaacac gcgtcaaact gccacgttca aagacaaaat catcgacgac
    14881 tacaaacagc cgcaaaccta cgcaggcttc cgcatcggac aaaacgtccg ccacgccaaa
    14941 tttggcaccg gcgtgattat cgatgccgca gataaaggcg aatccgcccg actgaccatc
    15001 aatttcggca aacagggcgt gaaagagttg gacaccaagt ttgcgaaatt ggaagagatg
    15061 tagaagggag atggcagagg ccgtctgaaa acgggaacaa caatttttgc ccaaacctcc
    15121 gccgtcattc ccgcgcaggc gggaatccag acctttcggc acgggaactt atcggataaa
    15181 aggtttcttt agattccacg ttctagattc ccgcctgcgc gggaatgacg gacggtaaaa
    15241 gccgggtata gatacctaac ccttgtttat agctgaaaat caataaggaa gaacattatg
    15301 tcccaagttt ttaaagattt tgatttgtcc tccgtatggg aatccgacag ttgggcagat
    15361 gaaaactaca aagaagcccc gtttacccct gaaattttgg ctgccgtaga aagtgaactg
    15421 ggctataaat tgccgcaaag ttttattgaa ttgatggcgg tacaaaacgg cggaatattt
    15481 gtcaaaaact gttttccgac cacacagaga aattcgtggg cgaaagatca tgtgcaaatt
    15541 tgcgaggtat cgggcatcgg ctttgaaaaa gaagggagtt tgtgcggcga aacagggcaa
    15601 aaactttgga tggaagaatg ggaatatccg cccatcggcg tgtattttgc caacgacccg
    15661 tcaggcggtc atgccatgtt tgccttggac tatcgggagt gtggaaaaga cggcgagccg
    15721 aaagtggtgt ttgtcgagca agaatcggat tttgaaatcg tcgaacttgc ccccgatttt
    15781 gaaaccttta tccgcagctt gcggcatgaa gatgagttta ttgacgagga aatataaaac
    15841 ggcggttgaa aaaccggaat catcaaggga aaacgggcga aataacggat aatcgcttga
    15901 atccgtaagg aaaacggttt ggtggaacgc gccatccaag acctttgcaa agaaaaactg
    15961 tctccgacag cattgacatt attaacagaa cttatcaagt ttggagctat ctcaaatata
    16021 attcggttat ccttttgtat ccattaaatc atatgcttca attaattgtt gttctagctc
    16081 ttgtaccaat tttgaattgc gaattcctga cacaatctca aattcttctg catctatgca
    16141 aacacctgca taaatttcaa taacaaggga acgcaataat tgaagctctt ctcttgttaa
    16201 agaaataata atgtcatcac ctgtgtaatt gattatattc ataataattt tatttttgtt
    16261 tgtcaaagta agttttgcct aaggttggtc taaatgcagt tccaccatct tttgaatttg
    16321 ggtctctgat tacaattgct ccagacttat catcccaaat tgctcttatg tgtttggatt
    16381 gtaatcttcg aattcccaag aaaaaaatcg taataagttt gaaagtgtca aatcccaagt
    16441 ttcttttgag caatattcta atattttatc aatttcactt ttaataatct tatgatcaaa
    16501 ctgttctaat attaatgcat tagaccaaaa aaaaccttct ttattacaat gatgggaaat
    16561 ccatttagga gaacaaatgc aaagtgaaaa aatagatgag ccttgttctc cttcgattcc
    16621 gatatccaaa tctatccatc tatggaaatt atctggaatt tcgggggtaa atttttcaaa
    16681 atcaatatca tataaattta tgctttttaa atccaattta atcattaggg ctgtcctaga
    16741 taaataggga aattcaaatt aagttagaat tatccctatg agaaaaagtc gtctaagccg
    16801 gtataaacaa aataaactca ttgagctatt tgtcgcaggt gtaactgcaa gaacagcaac
    16861 agagcctgac agcattgttt atacggattg ttatcgtagc tatgatgtat tagatgtgag
    16921 cgaatttagc catttccgta tcaatcacag cacacatttt gctgaacgac aaaaccatat
    16981 caatggaatt gagaattttt ggaaccgggc aaaacgtcat ttacgcaagt ttaacggcat
    17041 tcccaaagcg cattttgagc tgtatttaaa ggagtgcgaa tggcgtttta acaacagtga
    17101 gataaaagtt caaatttcca ttttaaaaca attagtaaaa tcgagtttat cttagttgtc
    17161 caggacagcc ccattatttt tataacaccg tgaagccgca cagcagtttg aacagtgata
    17221 cgccgtttgc gggcttacga gtttattttc ccggcctgca gtttgagcaa tacggtgatt
    17281 tcctacggtt aatacaaatg tttacacatt gatacatttc atttatagtt ccgcctattt
    17341 gaaaatagaa aatatgaatt cgaccgcaag taaaaccctg aaaggattgt cgctggtgtt
    17401 tttcgcctct ggattctgcg ccctgattta ccaggtcagc tggcagaggc ttctattcag
    17461 tcacataggt atcgatttga gttcgattac tgtcattatt tctgtattta tggtcggctt
    17521 gggtgtaggt gcgtatttcg gtggacgcat tgctgaccgt tttccttcaa gtatcatccc
    17581 cctgttttgc atcgctgaag tatccatcgg tctgttcggt ttggtaagca ggggtctgat
    17641 ttccggcttg gggcatcttt tagttgaggc tgatttgccc atcatcgctg ctgccaattt
    17701 cctcttattg ctgcttccta cctttatgat gggcgcgacc ttgcccttgc tgacctgttt
    17761 ttttaaccgg aaaatacata atgttggcga gtctatcggt accttatatt ttttcaacac
    17821 tttgggtgcg gcactcggat cgcttgccgc cgccgaattt ttctacgtct tttttaccct
    17881 ctcccaaacc attgcgctga cagcctgctt taaccttctg attgctgctt cagtatggct
    17941 gcgttacaga aaggatggat atagtgaaca ctaaaccgaa tactagtttg atttatatgc
    18001 tttctttcct tagcggctta ttgagcttgg gtatagaagt cttgtgggtg aggatgtttt
    18061 cgttcgcagc acagtccgtg cctcaggcat tttcatttac ccttgcctgt tttctgaccg
    18121 gtatcgccgt cggcgcgtat tttggcaaac ggatttgccg cagccgcttt gttgatattc
    18181 cctttatcgg gcagtgcttc ttgtgggcgg gtattgccga ctttttgatt ttgggtgctg
    18241 cgtggttgtt gacgggtttt tccggcttcg tccaccacgc cggtatcttc attaccctgt
    18301 ctgccgtcgt cagagggttg attttcccgc tcgtacacca tgtgggtacg gatggcaaca
    18361 aatccggacg acaggtttcc aatgtttatt tcgccaacgt tgccggcagt gcattgggtc
    18421 cggtccttat cggctttgtg atacttgatt tcttgtccac ccaacagatt tacctgctca
    18481 tctgtttgat ttctgctgct gtccctttgt tttgtacact gttccaaaaa agtctccgac
    18541 tgaatgcagt gtcggtagca gtttccctaa tgttcggcat cctcatgttc ctactgccgg
    18601 attctgtctt tcaaaatatt gctgaccgtc cggataggct gattgaaaac aaacacggca
    18661 ttgttgcggt ttaccataga gatggtgata aggttgttta tggggcgaat gtatacgacg
    18721 gcgcatacaa taccgatgta ttcaatagtg tcaacggcat cgaacgtgcc tatctgctac
    18781 cctccctgaa gtctggcata cgccgcattt tcgtcgttgg attgagtaca ggttcgtggg
    18841 cgcgcgtctt gtctgccatt ccggaaatgc agtcgatgat cgttgcggaa atcaatccgg
    18901 cataccgtag ccttatcgcg gacgagccgc aaatcgcccc gcttttgcag gacaaacgtg
    18961 ttgaaattgt attggatgac ggtaggaaat ggctgcgtcg ccatcctgat gaaaaattcg
    19021 acctgatttt gatgaatacg acttggtact ggcgtgccta ttccaccaac ctgttgagtg
    19081 cggaattttt aaaacaggtg caaagccacc ttaacccgga tggtattgta atgtttaata
    19141 ccacgcacag cccgcatgct tttgctaccg ccgtacacag tattccctat gcataccgct
    19201 atgggcatat ggtagtcggc tcggcaaccc cggtagtttt ccctaataaa gaactgctca
    19261 agcaacgtct ctcccggttg atttggccgg aaagcggcag gcacgtattt gacagcagca
    19321 ccgtggatgc tgcagcacaa aaggttgtct ctcgtatgct gattcagatg acggaacctt
    19381 cggctggggc ggaagttatt accgacgata atatgattgt agaatacaaa tacggcagag
    19441 ggatttaacc gtcttaaagg gtttcaggca acgcaggttt taggtaacgt cctgctagtt
    19501 caaaaaaacc gcatcacagc agtcgggaca aaatggttta aacattttgt cccgaattct
    19561 tattcctat
//
LOCUS       DAWFAD010000026        19179 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWFAD010000026 DAWFAD010000000
VERSION     DAWFAD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19179
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.026"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(239..2014)
                     /gene="ptsP"
                     /locus_tag="WGO49_000674"
     CDS             complement(239..2014)
                     /gene="ptsP"
                     /locus_tag="WGO49_000674"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ0305810.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYNAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRILLGMGLRRFSMNPNNILPVKNIILHSNIAQLENEIAKIVRCEDE
                     EKAEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(2014..2283)
                     /locus_tag="WGO49_000675"
     CDS             complement(2014..2283)
                     /locus_tag="WGO49_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ0305811.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2352..2789)
                     /locus_tag="WGO49_000676"
     CDS             complement(2352..2789)
                     /locus_tag="WGO49_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ0305812.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2870..3433)
                     /locus_tag="WGO49_000677"
     CDS             complement(2870..3433)
                     /locus_tag="WGO49_000677"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ0305813.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3502..4326)
                     /locus_tag="WGO49_000678"
     CDS             complement(3502..4326)
                     /locus_tag="WGO49_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ0305814.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLVSRQGYVFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     ASSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRYGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRVRTDR"
     gene            4418..5050
                     /locus_tag="WGO49_000679"
     CDS             4418..5050
                     /locus_tag="WGO49_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ0305815.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5257..7041)
                     /gene="rmuC"
                     /locus_tag="WGO49_000680"
     CDS             complement(5257..7041)
                     /gene="rmuC"
                     /locus_tag="WGO49_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ0305816.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFADLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQHDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERSNETTALSESLEYAAEDEAV"
     gene            complement(7380..8168)
                     /locus_tag="WGO49_000681"
     CDS             complement(7380..8168)
                     /locus_tag="WGO49_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ0305817.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8183..9532)
                     /locus_tag="WGO49_000682"
     CDS             complement(8183..9532)
                     /locus_tag="WGO49_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ0305818.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9551..10132)
                     /gene="petA"
                     /locus_tag="WGO49_000683"
     CDS             complement(9551..10132)
                     /gene="petA"
                     /locus_tag="WGO49_000683"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ0305819.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10255..11004)
                     /locus_tag="WGO49_000684"
     CDS             complement(10255..11004)
                     /locus_tag="WGO49_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ0305820.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11135..12064
                     /locus_tag="WGO49_000685"
     CDS             11135..12064
                     /locus_tag="WGO49_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0305821.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGAPLFERKSTPLRFTPVGERLLRLAHDLLPQVTDAERDLARIIEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPIWPQIELDIVSGFQADPIGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKTVWEAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            complement(12320..12712)
                     /gene="rpsI"
                     /locus_tag="WGO49_000686"
     CDS             complement(12320..12712)
                     /gene="rpsI"
                     /locus_tag="WGO49_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ0305822.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12722..13153)
                     /gene="rplM"
                     /locus_tag="WGO49_000687"
     CDS             complement(12722..13153)
                     /gene="rplM"
                     /locus_tag="WGO49_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ0305823.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13331..13510)
                     /gene="ssrS"
                     /locus_tag="WGO49_000688"
     ncRNA           complement(13331..13510)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WGO49_000688"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13516..13821)
                     /locus_tag="WGO49_000689"
     CDS             complement(13516..13821)
                     /locus_tag="WGO49_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ0305824.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13832..14161)
                     /locus_tag="WGO49_000690"
     CDS             complement(13832..14161)
                     /locus_tag="WGO49_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305825.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14216..15205)
                     /locus_tag="WGO49_000691"
     CDS             complement(14216..15205)
                     /locus_tag="WGO49_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ0305826.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15328..18030)
                     /gene="ppc"
                     /locus_tag="WGO49_000692"
     CDS             complement(15328..18030)
                     /gene="ppc"
                     /locus_tag="WGO49_000692"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ0305827.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRNMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEELARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAVLLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEGNPDTLTALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKIIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            18073..18966
                     /locus_tag="WGO49_000693"
     CDS             18073..18966
                     /locus_tag="WGO49_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ0305828.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINHNVDVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGCTQAAEALKILLDVGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGTER"
BASE COUNT         4113 a         5218 c         5091 g         4757 t
ORIGIN      
        1 aaagcgggaa tctagaacgt aaaatctaaa gaaaccgttt tacccgacaa gtttccgtgc
       61 cgacagacct agattcccgc ctgcgcggga atgacggatt ttaggtttct gattttggtt
      121 ttctgttttt gagggaatgg ccgattttgg gtttctgttt cggttttcta ttttgcagga
      181 atggcaaaat ttcagattgc gggcattgtt aagtatttct attttttacc tgccgtattt
      241 atttccgccc cttgaagtcg gcttcttcct cgacagacac gctgttcatc tgtttgatca
      301 gcttttccgc cttttcctcg tcctcgcagc ggacgatttt ggcaatctcg ttttcgagct
      361 gtgcgatatt gctgtgcagg atgatgtttt tgacgggcag gatgttgttc ggattcatgg
      421 aaaaacggcg cagccccata ccaagcagga tgcgggtaaa cgccgtatcg cccgccatct
      481 cgccgcatac ggacacgtct ttgtccatgc ggttggcggt gcggatgacg tgttgcagca
      541 ttttcagcac ggcgggatgt ccgggctggt aaagatggct gacgctgtcg tcgccgcgat
      601 cgacggacaa gatgtattga atcaggtcgt tggtgccgac ggagatgaaa tcgaccagtt
      661 tcaaaatact gccgacggtc agcgcggcag acggaatttc aatcatacag ccgatgccga
      721 ctttaccgaa ggcatcgccg cgttcggcaa gctggcgttg cgcggtgtcg aggtggatga
      781 ggcactggcg cacttcggat acggaggtaa tcatcggcca catcatgcgg acgggaccgt
      841 gtaccgccgc acggaggatg gcgcgcatct gggtgcggaa catgaccggt tcggcaaggc
      901 acaggcggat gccggtcatg cccagcgcgg ggttgaggct gccgttgggc gtgctgtttt
      961 tcccgaacca gcgcgggttt ttgtccactc ccaaatcgac tgtccgtatc gttacgcttt
     1021 tgcccttcat ttttttgaca atcgcgttgt acacttcgta ctgctcgtct tcagacggca
     1081 tcgtatcgcg gttgaggtaa agaaactcgc tgcggaacag cccgatgccg tctgcgccga
     1141 ggttgtgcag cggtttcacg tcttcggcgg attctatatt gcccacaagc tcgatgcaga
     1201 ccccgtcggc ggtggcggcg gcggtttttt tgagcttgtt caaatcgcgt ttgtggctgc
     1261 ggtattcgcg ggcacggcgg cggtattcgt tcaacaccga ctcatccggc gcgataatca
     1321 acacgccgtt gataccgtcc acaatgaccg tttcgccttc ggtaatcagt ttgcgcgcgt
     1381 tgtgcagccc gacgacggac gggatgtcca agctcctgcc caaaatcgcc gtatgcccgg
     1441 tggggccgcc ggcatcggta acgaaggcgg caatgcgctg ctctttaaac aaaaccgtgt
     1501 cggcgggcga aaggtcgttt gcaatcagaa cggtttcgtc aaacaggttg tcggcaactt
     1561 ccaactcgtt gccctgcccg atcaggttgt tgtggatgcg gcggacgact tgcagcatat
     1621 cctgcttgcg ttcgcgcaaa taggcatcgt ccatattgtc gaattgggcg gcgagtttgt
     1681 cgctctgctg cttcaatgcc cactcggcgt tgattttttg ttcccttaaa atatcgacgg
     1741 gttcgcgcga caaggtaaca tcggtcaaga gcatcaggtg cagcgagatg aacgcgccca
     1801 actcggtcgg ggcgttttcg ggaatcgcgc tgcggagctg ttccaactct ttgcgcgtgg
     1861 ctttgacggc ggcatcgaaa cgttcggctt cggcatcggt gtccgcctcc gcaacatcat
     1921 actgcggcac ttcctccgta ccgcgcgcaa tcaggtgggc gcaaccgacg gcaatgcctt
     1981 tgcccgccgc cacgccgtgc agcacgatac tcattattcg ccctcgccga agtagccgtt
     2041 gattaagtcg gtcagggcgc gcatcgcttc cgcctcgtcc gcgccgtcgg tttccagctc
     2101 gatgaccgta cccttggcgg cggcgagcat catcagcccc ataatgcttt tgccgttgac
     2161 gcggctgtcg tttttcgtaa cccagacttc gcttttgaat tgggacgcgg tttgggtgaa
     2221 cttgttggac gcgcgggcgt ggagtccgag tttgttgatg atttcgatgg attgtttgag
     2281 catttcggtt cccgtgttat gtatatcggc agcagacgcc gtttaaaatg ttttcctgcc
     2341 ctgccgcttc ttcagacggc atcgccgctg cgccggcaca ccaaatcttc gggcgcggac
     2401 gtgatggcga aaatgccttt tacagccgcc tctctgacac attcggtaaa ggcggcaagg
     2461 tcttccgccg ccggcgaata ttggacggcc ttaaccatca tcggcgcgtt cagcccggtc
     2521 aaaatcgccg atttgttttc gcgcacgagg cggcgggcgg cattgcaggg ggtcgcgccg
     2581 aagatgtcgg tcataatcag cacgccgtcg ttgtcgggaa attcctgaag cgcggcaatg
     2641 gcgttgttga tgatgtcgtc ttggtcttcc gtcggttgca cgccgagtat gcggacgttt
     2701 tcaggcagcc cgcccgggaa aaaatgatgt gccagcttgc ggtaggcttc gcctatggtt
     2761 tcgtgtgtga tgattaaaag ccctatcata ttatgcgtcc tgttcctcgt tgtcctgccg
     2821 gcgtatgggc gcaatgccgt ctgaaaggca ttcagacggc atcgcgtcct tattttccgt
     2881 ccaatgcgta aatctcgccc agattgcgcc agcagcccgc cgcatccatg ccgtaaccga
     2941 aaacataacg gttcggcaca tccagtccga cataatcggc tcggataggc ttgggtttgt
     3001 cgatcagctt gttggcgaac accgccgcac ggcagcttgc cgcacccatt tccaaaagtt
     3061 tggcttgaat ggcggacatc gtatgccctt cgtccaaaat atcgtccagc acgacgacgt
     3121 gcctgccccg gatttgttcc gcatcgggca tacgcttcca gttgaacgcg ccgccctcca
     3181 gcttgtcgcc gtaacgggaa acgtgaacat aatcaaaatc taagggaaaa cgcaacagcg
     3241 gcagcaactg ccccgtaaac accaccgcgc cgcccatcac gggcagcagc agcggatatt
     3301 tgccgcccaa atcgcgcgta atctcgtccg ccactttttg cagtgcggca cggcattggc
     3361 cttggtcgaa caaaagatcg gcgttttcaa gcatcgcctg tgtttcaagg cgtttggttt
     3421 ctaaatcggt catatgtcgg aatcggtcgg taaaaggaaa attataaacc aaagtatcgg
     3481 atgccgtctg aactgtcctg ctcagcggtc ggtacgcacg cgcacaaacg tggcgaattt
     3541 cggcgtgcct ttctgcgtaa agccacggta gcggtaggta atcagtgtgc cgattttggg
     3601 cgggttgtct cggtctttat ctttgaagcc gctgccaatg cggaattcgc cgtaccggtt
     3661 tttgcagccg accgcgccca accgtccggc gtttcgccct ttgccctcat agtgccgcgt
     3721 taccgtgcat tcgtcgtcgt attggctttt cagcttcaat aattggctgc tcctgccgcc
     3781 gctgtaacgg gattcgggct gacgcagcat cacgccttcg ccgccctgcg cttcgatttg
     3841 ttttaaaaag tccatcgcgt gctgccggtc gcgcactttg atttgtggga tgatggtaat
     3901 cggcgcgttc ggatgcgttt tcagccactg cgttgcgact gccaaacgtt ggtagagatt
     3961 gccctgcgcc ttgggtacat cgaaaacgtg caggcggata ccgcgccagt ctgaagaagc
     4021 agaacgcacg gtagcggaaa tctgctcgaa ctgaccgcgt ccgctataca attcgccgtc
     4081 caaaggataa ggcggaaact gcgcggtaaa gcctttgggc ggtgtaaata catatccttg
     4141 ccggctgact aaatgtttac cgtcccaata cgcgcgcacg ccgtcgagtt tctcgctcat
     4201 cgcccagcct gcaatgtcct gccctttgta ttcctgcgcc agcatcaaat ccgccgcgcc
     4261 tgctgatgca gggatgaaaa ccgccgtaaa aatcggtatg atgccgccga ttgtcttctt
     4321 aatcatctga ttcccccaat atcaaaacgg gcggcaaacc gccataaaac aaacggcaaa
     4381 cccgatgccg tctgaaaaac cgtttaggaa cacgccgatg accctacgtt acgaaatctt
     4441 ccccgttacc cccttccgcc aaaactgcac cctgatttgg gacgacgaaa gcggcgaagc
     4501 cgtcctgacc gatgtcggcg gcgacgtgcc gttcctgctg caagcgttgg caaaccgcaa
     4561 acttacgctc acggcaatct ggctgacgca cggccatctc gatcacgcgg gcggcgtggt
     4621 cgaaatgttg aaaacgcata aagtccctgt cctcgggccg catccggacg atgaattcct
     4681 gctccaatcg ctgccgcaaa ccaccgcgca atacggattt cccgtctcgc ccgcctttgc
     4741 gccgaaccgt tggctcgaag aaggcgaaac gctcacggtc ggacgctatg cctttcaagt
     4801 gctgcatatt ccgggccata cgccgggaca tatcgtcttt tattgtgccg aggcggaatt
     4861 gctgattgcg ggcgacgtgc tgttttacga aaccataggc agaaccgatt ttccgcgcgg
     4921 caaccacgcc gacttaatca ataatatccg caacaaatta ttcacccttc ccgaaaccgt
     4981 gcaagttgtc gccggacacg ggcgtatgac ttccatcgga cacgaaaagc ggcacaatcc
     5041 gtttttctaa ccgccttccc tacggtcttc agacggcatc atctgcactg atgccgtctg
     5101 aaacacaaaa ggctcagaca accgccgcct tgccggtgta cctcgccaga caaggctttg
     5161 gtaagtactt caaacaaacc caaaatcaga accagtacag ttacgccgcg ctttcggcat
     5221 tcccgccccg gctgaaacaa tatttttccg cacaagtcag actgcttcat cttctgccgc
     5281 gtattccaaa gattccgaca acgccgttgt ttcattggaa cgctcgacca aatcccgttg
     5341 aagttgtttg cctgccttca cgcccaacag acgcagtttc tcggcgcgtc cgaccagatt
     5401 cccgcgccct tcggcaagtt gcttgaatgc cgtctgaaaa ctgctttgcg cctgatcgat
     5461 gcctttgccg acgctttcga gcgtctgtac gaagccgaca aacttgtcgt acagcttgcc
     5521 gccttcgtcc gcaatcgcca gtgcgttctg attttgctgt tcgttgcgcc aaatattcgc
     5581 caccgtcctc aaagtcgcca gcagcgtact ggggccgacc agcataatcc gtttgtcgaa
     5641 acactcttgg aacaatcccg cgtcatgctg caacgccagc aggtaggccg gttcgacagg
     5701 gataaacata aagacgaaat ccaatgtgtt cacaccttcc aaatcggtgt aatccttcag
     5761 cgacaagcct ttcatgtgtg cacggatgct ggcaacgtgt gccgccagtt cgcgtgccgc
     5821 cgtatccgca tccgccgcct gcgtgtagcg cacataagct gtcagcgaga ccttggagtc
     5881 aatcacaatc tgcttgttgt cgggcaggtt gaccaaaacg tcgggctgga ggcggcgcgt
     5941 gccgccgtct tcctcttttc ggacggatgc cgcctgaacc acatattcgc gccctttctg
     6001 gaggccggaa ttttccaaaa ccgtttccag aatcatctcg ccccaattac cctgaacctt
     6061 gttctgcgtg ccggtcagcg cgttggtcag ggcctttgcc tcgctgtgca gctgcgcatt
     6121 caatccttga agccgtttca attcgttttc caacgtcagc cgctcgcgcg attctttatc
     6181 ataggtttgc ttgaccaact cgccgaaacc gtggatgcgt tcgtttagcg ggttcaaaac
     6241 ctgatggagc tgctcgcggt tctgctcggt aaaacggcgg cttttttctt ccaaaatcgt
     6301 gttggcaaga ttttgaaact gatcgctcaa gctcttgcgc gcctcgccca gtaaggacag
     6361 cttctcttca gaagcaaggc gttcctgttc gatttgcgtt gccaaacgtt cgttttcaac
     6421 cgccaaaccc tgtgcctttt cctgcaactc ggtatgcgac tgcctcaacc gctccgcttc
     6481 cgcctctttt tcctgcaaat gggcaatctg tttttcggct gcggcaaaac ggttgccgac
     6541 atcggcaagg tcgttttgca cgtcgcggac agtttggcgg ctttcttcca aatcggtttc
     6601 gattctttgg cggatttggc gttccaaggc atattggtcg gcaaacgcct tccgttcctg
     6661 tccgagctgc gttgccaaac gttcgttttc aaccgccaaa ccctgtgcct tttcctgcaa
     6721 ctcgatatac gactgcttca gccgaaccga ttccgcctct ttttcctgca aatgggcaat
     6781 ctgcttttcg gctgcggaaa aacggttgcc caaagcataa ttttcgtcct gcaaatgtcg
     6841 gtatttcccg tccaaaacgg ccaattccga tgcaatttct gcgtgtgcct gttcgacaaa
     6901 atcacatctt gccgcctttt ccgccagttg cgcgttcaaa tcggcaaact cgccctgaaa
     6961 cctgcccttc atcagcaacc atgtaaacaa cacgcccgac accaacgccg ccaaaggcag
     7021 caaaacagtc atcagttcca tcaatcatcc taatattcaa acattttcac accggacaaa
     7081 accgccgctt attccgattc tacctgtttg ttcgacatag ctccaaaaaa tatagcggat
     7141 tggctttaaa cctgttcgac atcgccttac catgctgctt gcggtttcag accttttcct
     7201 aattcaatat caatctgcca caaaccctga ttaagttccc gatgtctgac atttttagaa
     7261 tgatgccgtc tgaaatgttg cagctatgtt cagacggcat acggatttag gcttttcaaa
     7321 cggcaggcaa aatgaaaaaa gggcaaaccc taaaggattt gcccttttgt tccaaacgct
     7381 tagtgtacgt ctttccaata ttcttttttc aggaaatacg ccaaaggcag cataaccgca
     7441 aataggaaaa tcatcacgac atagcctata cgtttgcgtt gcagttgtgc aggttcgccc
     7501 atgtacacaa ggtaattgac caaatcgcgt acatatgcgt cgtactcttt ttggatcact
     7561 ttgccgttag gcagacggcg gctgtgcaaa ccggtagatt cccaatacag cttaggcttc
     7621 atctcgccgt gttcgtcttt taccataacc ggctgacctt tggcatccaa ctcaacggct
     7681 tgaacgcctt gctgttccca caacgggtgg ggcataccga ctttatcgaa tacagtattg
     7741 ttccagccgc tcggacgggt cggatcttta tagaagccgc gcatataagc gtaaaggtag
     7801 tctgcacctt tggaacgcgc aatcaatgtc aaatcgggcg gagcagcacc aaaccatttt
     7861 gccgcatctt tcgggttcat cgccgaatgc atgacatcgc cgacattatc ggtggtaaac
     7921 atcaggtttt tcttgatttc ttcgtcagtc aaaccgatgt ctttcaaacg gttgaaacgc
     7981 ataccgcttg ccgagtggca ggacaggcaa tagttggtaa agatttgtgc gccgcgctgc
     8041 aggctgactt ggtcgcgcaa atcgatatcg actttctcat agtgtgcatg accgcccgaa
     8101 gcaaatgccg cacccatggg tactgccaac agcaaggcag caaaccaatt tttcatagct
     8161 tgtttcattt tgactgccct catcagatgt tggttgcaaa caggtaagca cccacggcgg
     8221 taatgccgac gtaaacaaag aacatgattt tttgtttggc ggtgctcatg gttacgcgtt
     8281 cgggaaccgg tttgttttta tccagcttgg tatagaacgg catacccagg aagaatgcga
     8341 aatagacgaa ggacaggatg cgcgcgacca aagtacgcgt atcggttgcc accatcgcgc
     8401 ccaaaatacc caaaccgata aaggaaatga tgaacaagac caatgcggtt ttgaaaatcg
     8461 gaccgcggta acggacggat ttcacctcgc ccctatccag ccaaggcagc agggcaatca
     8521 acacgaccgc cgcgcccata ccgattacac cccaaacctg cgttcccaag aacgagggaa
     8581 tcgcacgcaa aatggcgtag aacggtgtga aataccatac cggcgcaatg tgcgggggtg
     8641 ttttcagcgc atttgccgca tcgaagttcg gcgcttcgag gaagtagccg ccgccttccg
     8701 gtgcgaagaa caatacggca cagaagacaa tcaggaatac aaccacaccc aagatgtctt
     8761 taacggtata gtaaggatgg aatgggatac cgtcgcgcgg gataccgttt tcgtctttgt
     8821 ttttcttgat ttcaacgccg tcagggttgt tagaacccac ttcgtgcagg gcgatgatgt
     8881 gcgccacaac caagccgagc aataccagag gtaccgcgat aacgtgcagg gcaaagaaac
     8941 ggttcagggt aacgtcggaa acgttgaagt cgccgcgaat ccaagtggac aaatccggac
     9001 cgataacagg gatggcggag aacaggttaa taattacctg cgccccccaa aaggacatct
     9061 gaccccaagg cagcaggtag cccataaagg cttccgccat caatgccaag aaaatcagag
     9121 aaccgaaaat ccacaccaac tcacggggtt ttttgtacga accgtaaatc aaaccacgga
     9181 acatgtgcag ataaacaaca atgaagaaga aagatgcgcc ggtggaatgc atgtagcgga
     9241 taatccaacc gccggacacg tcgcgcatga tgtactcgac tgcagtaaag gcagcaggca
     9301 gatggtaggc gttcaggttg ccgtcgggtt tgtagttcat ggtcaggaaa atgccgctga
     9361 cgatttgaat cacgaggaca agcagggcca aagagccgaa gtagtaccaa aaattaaagt
     9421 ttttcggtgc gtagtattga gccaaatgct cattccacat tttagacagg gggaaacgag
     9481 cgtccatcca gcctaacaat gcttttgctt tgctattggt ttggtttgcc ataattatcg
     9541 ttccttattc ttagtcttcg cccaccaaga tagttgtgtc gctcaagtat ttatatggcg
     9601 ggacaaccag gttggtcggg gcaggaacac ctttatatac gcggccggcc aagtcgaatt
     9661 tcgaaccgtg gcaggggcag aagaagccgc ctttccagtc tgcacccaaa tcggcggggg
     9721 caatgtcggg acggaaggtg ggcgagcagc ccaaatgggt gcagataccg atggcgacaa
     9781 ggatgttcgg cttaatcgaa cgggtctcgt ttttagcata ctccggctgc tgttccgcat
     9841 cggaattggg atcggtaagt tcgccgttca ggcctttcag gtctttaagc tgctgatctg
     9901 tacggttgag cacccaaatc ggtttgcctt gccactcggc ggtcagcagc tgacccgctt
     9961 cgattttact gacatccacc tcgacggcag caccggcggc cttggctttt tccgaaggga
    10021 aaaaactggc cacaaacggc gttgccacac ccaatgctgc cactccgccc gcgccgcagg
    10081 tcgcgagtgt caggaaacgg cggcggccgt tgttgatttc ttgattatcc attattcagt
    10141 cgtcctaata ttttgggaat accgagccat taaacgttgc aattttaccc agtttgcagt
    10201 gatactcaaa gcattattta aaataaggta aagttttatg atatttctca agactcaagc
    10261 cggattgttt tcgtcaaaat ggcacacttc caacccgaaa acctctgccg ccgattctgc
    10321 cagcgcgcgt acgccgtaac gttccgtcgc gtgatgccct gccgaaatga aagccgtacc
    10381 cgtttcattg gcaaggtggt attgggcttc ggaaatttcc cccgtcaaat acagatcgac
    10441 accttcgtct attgccgtct gaaaaaaccc ctgcgccccg ccgctgcacc atgcaacccg
    10501 tcggatttcg cgttcgggtt tgccgatgac gacaggctta cgttgcaaaa ctgtttcaat
    10561 atgcgccgcc aatgcgccga gtgtcttggc ttgtttcagg ctgcccgagt tgagcaggtt
    10621 ttgttcgccg aaccgttttt ctgtcgcaaa acccaatctg tcggcgagtt gggcattgtt
    10681 gcccagtgtg ggatgtgcat ccaggggcag atggtagcct gccatattga tgtcgtgccg
    10741 taacagtgcg gcaatccgtt cttttttcca accagtaacg gtcggcaact cgtttttcca
    10801 gaacataccg tgatgtacca aaagcaaatc tgccttctgc tccacagcaa aatcaatcgc
    10861 tgccctgctt gccgttaccg acgtaacgat tttcccgata tattccctcc cttcaacctg
    10921 caaaccgtta ggggcgtaat ctttaaacaa cgctgtctgc aatgtttcat tacaccaagt
    10981 cagaaaatcc ctgcgcaata ccatcttttt tcctaatcgc tttaaacaag cgggcattct
    11041 aatcgcaaaa tgtctggaat tcacattttt ccgatttgca cccgcatatg aattatttta
    11101 atatgcgccg gttcaatatg ccgtctgaaa cctcatggat tccatcatcg aactgcgcca
    11161 cctcaagacc ctgctggcac ttgaagaaac cggcagcgtc tcccttgccg ccaaacgggt
    11221 tttccttacc caatctgcac tttcccacca aatccgtatg cttgaaaacc actacggcgc
    11281 accgttgttc gaacgaaaat ccacgccctt gcgctttacc ccggttggag aaaggctgtt
    11341 acgcctcgct cacgacctgt tgcctcaagt taccgatgca gaacgtgatt tggcacgcat
    11401 tatagaagga gaggcaggag aattgcggat tgccgtcgaa tgccatacct gtttcgactg
    11461 gctcatgccc gcaatgggcg aattccgccc gatatggccc caaatcgaat tggatatcgt
    11521 atcgggattc caagcggacc ctatcggact gctgctgcaa caccgtgccg accttgccat
    11581 tgtttccgaa gcggaaaaac aaaacggtat ttctttccaa ccgctgtttg cctacgaaat
    11641 ggtcggcatt tgcgcaccag accatccgct tgccgccaaa actgtctggg aagccgaaga
    11701 ctttatcggg gaaaccctga ttacttatcc cgttcccgac gagatgctgg atttgcccaa
    11761 aaaaatcctg attccgaaaa acatcaaccc gccgcgccga cacagcgagc tgaccatcgc
    11821 cattatccaa ctggttgcca gcagacgtgg cattgccgcc cttccctatt ggacagtcat
    11881 gccctacctt gaaaaaggct atgtcgttca ccggcagatt actgccgacg gattgcaaag
    11941 caaactgtat gccgccatcc gtaccgaaga tacggacaag agctatctga acaatttttg
    12001 ccaaatcata cgcgaacgcg gttttgcaga tttgcccgga ctgagcgaac tggaaccggt
    12061 ctgacccctt attcaaccat acccggcagt ttttctattt tttcatgtat agtggattaa
    12121 caaaaaccag tacagcgttg cctcgccttg ccatactatt tgtactgtct gcggcttcgt
    12181 cgccttgtcc tgatttttgt taatccacta tactgttttt gatttttgcc caatctgtaa
    12241 tctttagatt gccaatggga aaccgtctac tacaaataaa aaaaccctgc gataagcagg
    12301 gttttttgaa tttccaacat taacgtttgg agaattgttt tgcacggcgt gctttgcgca
    12361 gacccggttt tttacgttcg acttcgcggg catcgcgggt aacaaaaccg gcttgagaca
    12421 aggcgggttt caacgcggca tcgaagtcga tcagggcacg ggtaatgccg tggcggattg
    12481 cgccggactg gccggtttcg ccgccgccaa caacattgac tttgatgtcg aaagattcgg
    12541 cgttttcagt cagaaccaag ggttggcgaa caaccattcg gctggtttcg cgtgcgaaga
    12601 attcgtcaac gggacggccg tttacgatga tttgacctgt acctttaatc aggaatacac
    12661 gagccactga acttttgcgg cggcctgtgc cgtagtagta tttaccgttc atgtcgcgtc
    12721 cttatttcag ttccaaaact ttgggttgtt gcgcagcatg ggcgtgttcc gcacccgcat
    12781 acactttcag ttttttaatc atggcgtaac ccaaaggacc tttgggcagc atacctttta
    12841 cagcttgttc caaagcgcgg cccgggaatt gctcttgcat ttcgcggaaa gtgcgttcgt
    12901 agataccgcc tgggaaaccg gaatggcgga agtatttttt atcttcgaat ttggcaccgg
    12961 ttacacgcag tttgtccgca ttgataacaa tgatgtaatc gccggtatcg acgtgggggg
    13021 tgtattcagg tttgtgtttg ccacgcagac ggctggcgac ttcggccgca acgcgaccca
    13081 agactttgtc ttgggcatcg atgacgaacc attcgcgctt cacctcgtgg ggtttcgctg
    13141 aaaaggtttt catagtggaa atccaaatag atatagaaag ttgtaaattt taaagacagg
    13201 attcgatttt gtcaatcgca ttaccgcgtt acggaaggat tttccggatt tcggcagact
    13261 gcatactgct ttttcgggcg gggcggcggc caatgtgaaa aaccgcatcg ttgcgatgcg
    13321 gttttgaatg ggaatccccg cgagagccgt ttcggccgaa tccgcttgaa ccttgctgac
    13381 aaggcggctg cctcgggtag tttcgggtgc gtccgcaaaa ggacgctcgc gcccactact
    13441 gctcccggca accttaagcg aacttattgg ttcaaaggaa tatatgcctt cgcggacacc
    13501 gcagggaaaa aggggttatt cctgcgccaa gcgggatagt gctttttggc aggcgttgtc
    13561 catatcggct attttacgcg caaaatcgcc gattgccaaa tcgccgccgt tcagggaggt
    13621 tttcaacagg tcgtggacga cgttgagcgc ggccataatg acgatttttt cgctgtccgc
    13681 gacgcgtccg ccttcgcgga tggcttcggc tttgccgttg agcattccga ctgcctgcaa
    13741 cagtgtgtct ttttcttctg ccggcgtgtt gacggtcagc cgggcgtgca tgacttcgat
    13801 gtggacttgt tcgatgttca tcctttaatc cttattgctg cgtttcctgc cattggggga
    13861 ggcgcgctgc cagtgcgctg attttttccc tgctctgttc gagcaggctg cggtatcgtg
    13921 tattttcttc tgtcaggctg tcgattttgt tttgcaggtc ttctttgagt ttgccgactt
    13981 ggacgagcag ggcttcgctg agttcgtcga cggcggtttc gtgttcgagt ttttgccgct
    14041 cgtgcgcccg tttgagttcg gcgacggttt ctttgaggcg gcggttttcg ctgacgaggg
    14101 tttcgaattt ttgtaccagc gtataaacgc tgctttcgag tttttcgata ttttgtttca
    14161 taaccttacc tgtccgtatg ccgtctgaag gcttcagacg gcatctgtct gttgtttatt
    14221 caaaacgcgc gctgcgttcc atcagccttt cgacgacctg ttgcggggtc atttctttgc
    14281 ggatgagttg cagcagggtt tgggtaatcg gcatgtcgat ttggtactta caggcagtat
    14341 tgaagacttc ttctatcgtg ctgacccctt cggaaacgtg tccgatttcg accagcacct
    14401 gatgcagttc cttgccttct gccaaaccca agccgacgcg gcggttgcgt gagagtgcgc
    14461 cggtgcaggt caagatgagg tcgccgatgc ctgccagccc catcatggtt ttgggctgtg
    14521 cgcccattgc ggaggcaagg cgggtgattt cagctaatcc gcgcgtaacc agtgcggcac
    14581 gggcgttaag cccgtactct agaccgtcgg acaatccggt ggcaatcgcc ataacatttt
    14641 ttaccgcgcc gccgactgcc acgccgataa catcggtact gccgtaaagc ctcatgacgg
    14701 tcgtgttgag ctgcggtacg agttcttcaa tccactcttg gttttcggag gcgaggacga
    14761 cggcgcaggg cagttgtttg gcgagttcct gtgcaaaact cggaccggaa agtacgccga
    14821 tttttttatt gtcgggcaat acttctttca atacttgaaa ggtcagcagc cctgtatcct
    14881 gctcgaatcc tttgcaggcg gcgaggacgg ggaggtgtcc cgcgccgtac tgtttgagca
    14941 gctctgcgct gcttctcaat ccggcaacgg aggttacgat aaggacaagt ccgctgtctt
    15001 tgagcgcgtc tgccaaatcc gcacacactt ccaaggtttc gggaaaggaa aagccgggca
    15061 gtccgcgttt gttttcacgc gcttcctgca tttggcggac ttggtctgcg ttgcgcgtcc
    15121 acagggatac gcggttgccg tgttgggaaa agtgcagggc gagcgccgta ccccacgaac
    15181 ctgcgccgat aacggtaatt ttcattggtc gtctttcaac atatcactgc cgttcacttt
    15241 aaaacaatcg gtgtttctct gcaagtgcgg tcagggaaat gccgtctgaa aggcgttcag
    15301 acggcatttt gccccgatgc ggcactatca gcctgtattg cgcaaacctt gcgccacgcc
    15361 gttgatggtc aggtgcacca tcagaagggc gtgcggattg tcgggttctt tacgcaggcg
    15421 tttgagcatg gcgacttgca aaccgttgag cgcgttcagg tatggaatcc tcaaagcgag
    15481 cgaacgggcg aggctgcggt tgtcgcgcaa aagctcttcg gtttgcagca ggtcgagcag
    15541 tgctttgcgg ctgcggcggt attcttcctt aatcatcccg aagatgattt ttgccttatc
    15601 gggcgattcg ctcaagccgg catagttttc cgcgagggtg atgtcggttt tcgccatcac
    15661 ttgctccata ttggagagca tggcttggaa gaacggattg ctttgggcat gttcgcgcag
    15721 ggcggtcagt gtgtcgggat tgccttcgca caaggtttcc accgcgctgc cgaaaccgta
    15781 ccaagcaggc agcatgaggc ggttctgcat ccaggaaaat acccacggaa tcgcgcgcaa
    15841 gtcttgaatc cgcgctaagg tcttgcggct ggcaggacgg ctgccgaggt tgagggtggc
    15901 gatttcctga atcgggctgg tttgcaggaa gtagtcgatg aagtcgggat gggtaatcag
    15961 ttcgcggtag tatttgaacg atacgtccga caatgcctgc atcagtttgg catcagggtc
    16021 ttttttatcc ggcaggatgc tggcttccaa agtcgcggca accaaggttt ccaagttgcg
    16081 ttgggcattg ccggggtcgg cgtatttggc ggtaatgact tcgccttgtt cggtaatgcg
    16141 gatttgtccc gccacgctgc ccgccggttg ggcgagaatg gcttggtaag acgggccgcc
    16201 gccgcgtccg acgctgccgc cgcgaccgtg gaacaggcgc atgcggacat cgtatttttt
    16261 gaagagttcg accaagccca attccgcctg atacagacac catgagctgg taacatagcc
    16321 gccgtctttg ttggagtcgg agtagccgag cataatttct tggatgttgc cgcggctttc
    16381 gagcagggca tcgtaccaat cgaggcggaa catggtttcc atgaccggac aggcgttttc
    16441 gagtgcttcg atggtttcaa acagcggcac gatattgatg cggctgtgcg gtttgccgtt
    16501 ttccaccgcc aacaggccgc tttctttcag cagcaatgcc aaggcgagca ggtcgctggg
    16561 ttgttcgcag ttggaaataa tgctttgtgt tacggcatct tcgccaaatt cgtctttgat
    16621 tttgcgcgct tcgttgaaaa ttgccagttc gtggcgggta tggtcgctgt atgtgatgaa
    16681 cgggctgtac agcggacgtt gatggctcaa ttctcgcaac aggacggctt gtttttgttc
    16741 ttcgttcagg ctgttgtagt cttccaagcc tgcgtgttgg aaaagctcgg caaccacatc
    16801 ggcgtgtttg cctgcgtgtt ggcgcaggtc gagcggcatc atgtggaagc cgaacacgga
    16861 cacggaacgg atgaggtctg ccaaacggcc ttcggcaagc aggcggctgc cgttgtcgat
    16921 aagggaacgt tgcaattttt tcaaatcatc gagaaattct tgtgccgaag catacggctc
    16981 gagaaagccg aatttgcagc ccatgcccaa accgagcgcg cgcgctttgc ccatagcgcg
    17041 cgccatgatg taagcaatgg cgcggcggta gggttcttcc gtgcgggcaa gttcttcgtc
    17101 aggcgagagg gctgccagtg ccattacgtc gtcgttgact ttgacgcggc ggatggagag
    17161 cggcagttcg cggtaaagtt tgtcgagttc gccacggtaa aaacggaaca cggcatcggc
    17221 gtggcggcgg aaggcaaaac gcaaggtttc ggcagaaacg aacggattgc cgtcgcggtc
    17281 gccaccgatc cagccgccga ttttgaggat attcggaacg cagacatcgg gatagaccat
    17341 ctgaaagtcg tgttccatat tgcggtagag cttgggcagg gcttcgaaaa agctcatcgg
    17401 gaagatggac acgccgttgt tgatttcgtc gttgacgctg agtttgtggc ggcgcgtttc
    17461 gctggtctgc cacaagccca gcaggatagt gtcgatttcg cggcgcagcc gtgccagcgc
    17521 gtcggcattg gtgcagcgtt cgcgttgcgg caacagtgcg cggatgcggc ggttgaagct
    17581 taagacggtt tggcgttgca cttcggtcgg gtgcgcggtc aaaacggcgg taacggacgt
    17641 attgtccaac tgccgctgca ccgatttgcc gtcggctttc cccgctttga gcctgcggac
    17701 ggtttccgtc aggctgcctt ccgcgccgcc gcgtccggct tcttcgtgga tttggcggcg
    17761 gcgttcgtgg tgcacgtctt cggcgatatt caaaatctgg gcgaacaggc cgcaggccaa
    17821 ggttaaatcg tgggtttgtt gttcgtccaa ttgcggcaat actttttcaa tcaatgccgc
    17881 gctgtcgtcg gaagtggaca agagtttgac cgtttcgaca accaatggcg aggcttcttc
    17941 gtgcaggagg ttgaacaggg attgtttcag aaattccgcg tccgccgcca aagccgcgtc
    18001 cttcggattg ttcagaatat gcagttgcat gatttttctc tcattgccgt aaatactgta
    18061 aatgtacctc aaatgccgca tccgtgccaa accgttcaca ctttaaccgc ccgtgtcccg
    18121 aaatgccgtc tgaagttgaa cgccgcccga cggcagcgtt acaatcgccc acaactgttt
    18181 tttccgaaca tcatcatgac cacgaccgaa cacgacaacg acgatgcatt cctgctgcgg
    18241 tacagccgcc acatcctctt ggacgaaatt ggcatcgaag ggcagcagaa actttccgcc
    18301 gcgcatattt tggtcgtcgg ctgcggcggt ttgggtgccg ccgccctgcc ctatctcgcc
    18361 gcttccggca tcggcacgct gaccatagcc gattccgaca cggtcgagct gcacaacctg
    18421 caacgccaag tcgcatttga cgagggcgat gtcggcaagc ccaaagccga agccttggca
    18481 gaccgcctga aacgcatcaa ccataacgtc gatgtccgca ccgtcaacga aaaactcgac
    18541 ggctgccgcc tgaccggttt ggttcaaacc gccgacatcg ttttagactg ctgcgacaac
    18601 tacgccacgc gccaagccgt caaccgtgtc tgcgtacaaa cgaaaacacc gctggtttca
    18661 ggagcagcgg tgcgctttga agggcaactt gccgtgtacc gtcccgactt gcccgactcg
    18721 ccgtgttacg cctgcctgtt tgacggcgga tcggcttcag acggcatctg ttccctcttc
    18781 ggcgtattct cgccgctggt cggcatcatc ggctgcacgc aggcggcgga ggctctgaaa
    18841 atcctgctag acgtgggcga accgtcgcac ggcaggctgg cggtttaccg tgccttggaa
    18901 gggggctggc aatatttcga cctgccgcgc aaccccgaat gcccgatttg cggcacggaa
    18961 cggtaaaacc ctgccgccgt ttcagacggc ataaagacgg atgcggagga acggttttaa
    19021 aaatcggcgg tgttttgaca tagtgtcgat aagcatactt atcttcaata tactcaacag
    19081 gataattgtt tgcctaagca aaaaataatc gccttttccc tgacgggcgg gcgaaatcca
    19141 cggattgcaa aaacacaacc tgaaaattta catttcttt
//
LOCUS       DAWFAD010000027        18845 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWFAD010000027 DAWFAD010000000
VERSION     DAWFAD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18845
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.027"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            52..1005
                     /locus_tag="WGO49_000694"
     CDS             52..1005
                     /locus_tag="WGO49_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ0305829.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVSKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1109..1432)
                     /locus_tag="WGO49_000695"
     CDS             complement(1109..1432)
                     /locus_tag="WGO49_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305830.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1605..2966)
                     /locus_tag="WGO49_000696"
     CDS             complement(1605..2966)
                     /locus_tag="WGO49_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ0305831.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASQEQRTEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVEGT
                     DWE"
     gene            complement(3327..5027)
                     /gene="recJ"
                     /locus_tag="WGO49_000697"
     CDS             complement(3327..5027)
                     /gene="recJ"
                     /locus_tag="WGO49_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ0305832.1"
                     /translation="MSVKIQTRSINTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLAALLPYQTLTNCEAAAGRLADAVGRKEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPADTVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLTALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDSEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTTFEEAVRE
                     MVCEDDLSQTFITDGSLPACDITLEQAQNLARHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
     gene            complement(5079..5813)
                     /locus_tag="WGO49_000698"
     CDS             complement(5079..5813)
                     /locus_tag="WGO49_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305833.1"
                     /translation="MLFRKTTAAVLAATLMLNGCTLMLWGMNNPVSETITRKHVDKDQ
                     IRAFGVVAEDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTDGLCLRYDTDRPDDIAKLKQLEFEAVELDKQTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTEKHTDKSKLFANILYTPPLFDTGCG
                     GRGTGFACGGSGCGRGCRPQMNSNAV"
     gene            complement(5860..6366)
                     /locus_tag="WGO49_000699"
     CDS             complement(5860..6366)
                     /locus_tag="WGO49_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ0305834.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAVVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPHADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            6521..7852
                     /gene="pmbA"
                     /locus_tag="WGO49_000700"
     CDS             6521..7852
                     /gene="pmbA"
                     /locus_tag="WGO49_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ0305835.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGFAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            complement(8149..8352)
                     /locus_tag="WGO49_000701"
     CDS             complement(8149..8352)
                     /locus_tag="WGO49_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0305836.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8595..8897
                     /gene="clpS"
                     /locus_tag="WGO49_000702"
     CDS             8595..8897
                     /gene="clpS"
                     /locus_tag="WGO49_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ0305837.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8899..11196
                     /gene="clpA"
                     /locus_tag="WGO49_000703"
     CDS             8899..11196
                     /gene="clpA"
                     /locus_tag="WGO49_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ0305838.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDEDDGNYSSDG
                     MDDDNENRAKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPL
                     LVGEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKS
                     VLKQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEY
                     RTIFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELS
                     ARYINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVSRIPEKTVSH
                     DDKQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGK
                     TEAAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHC
                     VLLLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSL
                     GFTAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLD
                     KKVEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRI
                     DWDAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(11260..12264)
                     /gene="rfaD"
                     /locus_tag="WGO49_000704"
     CDS             complement(11260..12264)
                     /gene="rfaD"
                     /locus_tag="WGO49_000704"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ0305839.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LNWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVTFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYKEEFFDVKSGVDRYVKWMLENLA
                     "
     gene            complement(12305..13423)
                     /locus_tag="WGO49_000705"
     CDS             complement(12305..13423)
                     /locus_tag="WGO49_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0305840.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     REKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEVLLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFDQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(13425..14660)
                     /gene="dcm"
                     /locus_tag="WGO49_000706"
     CDS             complement(13425..14660)
                     /gene="dcm"
                     /locus_tag="WGO49_000706"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0305841.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFSRVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(14697..15668)
                     /gene="hldA"
                     /locus_tag="WGO49_000707"
     CDS             complement(14697..15668)
                     /gene="hldA"
                     /locus_tag="WGO49_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ0305842.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(15723..16463)
                     /gene="pyrF"
                     /locus_tag="WGO49_000708"
     CDS             complement(15723..16463)
                     /gene="pyrF"
                     /locus_tag="WGO49_000708"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ0305843.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(16989..17636)
                     /gene="adk"
                     /locus_tag="WGO49_000709"
     CDS             complement(16989..17636)
                     /gene="adk"
                     /locus_tag="WGO49_000709"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ0305844.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            17851..18690
                     /gene="htpX"
                     /locus_tag="WGO49_000710"
     CDS             17851..18690
                     /gene="htpX"
                     /locus_tag="WGO49_000710"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ0305845.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGTGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
BASE COUNT         4602 a         5114 c         4522 g         4607 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatcttgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggc tttctgcccg gagacctgac ccaaaaagtc
      601 gacccctacc tccgtccgct ttatgacgcg ctttacgacc tgatgggctt cgaccgcgtc
      661 agcaaactga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 aacgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctctg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa tcgggcagag aaaaacggat acggaaacca agctccgtat
     1081 ccgtttcttt atccgtccaa ccgatacatc aatcgaattt tctgcatcgg attgcctcat
     1141 cttcgcaaaa cattgccgaa gtttcgcggt tgcacgcctg acgcactttc tgccgcgcca
     1201 cgccttcatt ccgatcgctt gcctcaaacg tcttctgaaa aggcgtaacg ctgcatacat
     1261 acatggtgga atttgaaccg aaaaggtcga ttttgacggt attgctgccg ctttccaata
     1321 cggcgacacg gtgttccaac tgcgccagac gtgcttccaa atcgccgatt ctgtcggcaa
     1381 atgcaggcat ggcggcacac gcggtcaaaa tgcccaaaat acattttttc atcaattttt
     1441 ccttgtttct tgaaccctac ccacccggac atcctttttc cgagaggcag ggtcagactt
     1501 tatttacggg gatgaatccc cttacaaatc aggataatac atagaacggc actttatgct
     1561 tcatcccatg tgttgaaact tcattgctcc ttgtctgttg acccttattc ccaatccgtt
     1621 ccctcaacct tcggcttggg tttgcgcctg cgacggcggc gttttttcgc ctgtccttcg
     1681 tttttttcgt gccgggcagc agcctcgttt ttggtcattt ccgtccgttg ttcctgcgat
     1741 gccgtctgaa acgccgtcca ccactcggca agggcgcggt ccgcattgcc ggtttcggcg
     1801 cgcaagagca ggaaatcata ggcggcacgg aaacgcgcct gtgcaaacag tttatgcgga
     1861 cgtgcgcctt tgcggttctc aaactgcggc tgaaacatcc aaatttcgcg catcgtggcg
     1921 gaaaagcgtt gcggcacgcc ccaaccgcgt tcgacggttt cacgcatcgt attgattgca
     1981 tcggacaggg cgggcgcagg tttcaaaccc tgttgcagat tgcttttcca atggcgttcc
     2041 aactcgggcc acatcagagc cgccagtaca aaaccgaccg aaaccgattt gtcagcacgc
     2101 agccgctcat cggtattttt cagggcaagc accgtcattt ttccggcgat gccgtctgaa
     2161 acgcgcaagg cattgagcag cggatggatg tcgtccggta tgtcaaatcc gttcaaacgt
     2221 ttcagacact cgcgcgcgtg ccctgaaaac agcagtttca tgatttcgtc aaacagcctc
     2281 gctaccggtt cgtgcttcag acggcatatc gattcggcaa tcggtgcggt ggtttcttcc
     2341 gacagctcaa agcccaattt gcccgacagg cggatggcgc gcaaaatcct gacagggtct
     2401 tcctgatagc gttcggcggc atcgccaatc ataaccagcc tgcgggcggc aacatcggca
     2461 atcccgttgt ggaaatccaa aatctcttct ttttcaggat cgtaatacaa ggcattgcag
     2521 gtaaaatcgc gccgcatcgc atcttcttcg atgctgccgt aggtgttgtc tttcatgatt
     2581 ctgccgtgcg cgttctgatg aaccttcgca ccgccgcgaa acgtcgttac ttcgataatc
     2641 tccgcaccgt tcatcacatg gacgatttga aaacgcctgc cgatgatgcg gctgcggcgg
     2701 aagagtttgt gcacctgttc gggcgtggca tcggttgcga catcgaaatc tttgggttcg
     2761 atgccgagca gcagatccct gaccgcgccg ccgaccacat aagcctgaaa ccccgcccct
     2821 ttcagacggc gtatgacgtt ttcggcggca aagctcaaca tttcggcacg gatgttgtgt
     2881 ctttcggcag gaatgaccgt tttactttcc gcttttttac tgctccgacc ggaaggcagc
     2941 atcttattca accatttttt cagcataaat attctaaccc gaagagcggc atctccacgg
     3001 gcaaccgtgt tcagacggca tcaggcggta cgattccgca agatgccgta accataaaca
     3061 aaaggcgtag aaactacgcc gtccgaatca cgccgccctc gcggcggcgg caacgcgcca
     3121 ttataacaga ttgcccttta aaaaaatcag accctgcttt tgtggcggag tctgaaattt
     3181 gatgcacctt atatattgcg tccctaagaa gggatgatta acaaaaatta acgcccttta
     3241 ctttcttttc cgattgggaa cattcccgtc gtacaaacag gcaaatgaag gaacagaaat
     3301 cacattcaaa cggcgttccg ccgcctctac accgcttccc agtaatcgat atacagctgc
     3361 aattcgagat tgttgcgcca ttcgttggca acggggcggt aaaccgtgcg gatgtattcg
     3421 gggatgtctt cgctgcaacg ccaaaacatc gcttcaaatt cgcagccgtc tttttgcagc
     3481 cagacttttt tgtgtttgcc ctccgcgccc aaaggttgct ggcggacgac gtggaactcg
     3541 tcggtaaagc tcggcggcgc gaagccctgc ccccaaacgt gacgggcaag gttttgcgcc
     3601 tgttccaacg tgatgtcgca ggcgggcagg ctgccgtcgg tgatgaaggt ttgcgacaaa
     3661 tcgtcttcgc acaccatttc gcgcacggct tcttcaaagg tcgtctgaaa cgcgggaatg
     3721 ttgtgttcaa gtatgctcaa acccgccgcc atcgcgtgtc cgccgaattt taaaatcaaa
     3781 tcgggatggc gtttggacac caagtccaaa gcatcgcgca ggtgcaggtt gggaatggaa
     3841 cgccccgaac cgcgcacttc gccgttgtcg gcaggcgcaa acacgatggt cggacgataa
     3901 aaacggtctt tgaggcggct ggcgacgata ccgaccacgc cttgatggaa gtcgtcgcga
     3961 tacgccacca aagtcgtctg acctgaaggc agggtttcag gaaaggcgtt cagcgcgtct
     4021 tgcagcatag actgctcgat ttcgcgacgc tcgatattga ggttgtttaa ctgagccgcc
     4081 agttcctgag cttcggaatc atctcgcgcc aacaggcagg cgatgccgac cgacatatcg
     4141 tccagccgtc cggcggcatt gatgcgcggg cccaacgcaa aacccatatc aaacggctgc
     4201 gccttgcgcc agtcgcgccg cgccacttca aacaaggcgc ggatgccggg gcgcattttg
     4261 cccgaacgca tccgtttcaa accttgcgac acgaggatgc ggttgttgtg gtcgagaggg
     4321 acgacatcgg caaccgtgcc gagtgcgacc aaatccaaaa gatcgcccag attcggctct
     4381 ttcaggccgt ctgaaaaata attgcggcgg cgcagttcgg cacgcaacgc cgtcaacaca
     4441 taaaaaatca cgcccacgcc cgccaagctt ttgctttgga aaccgcagcc tttttggttc
     4501 ggattgacga tgatgcagtc gggcacggta tcggcaggca gatggtggtc ggttacaatc
     4561 acatccaaac ccagagcctg cgccctcgcc acgcctgcga tgctggcgat accgttatcc
     4621 accgtaatca gcaaatccac gccctgcgcg gcagcgattt cggcaagttc gggcgttaag
     4681 ccgtagccgt gttcaaagcg gttgggcaca aggaaatcca cttttgcccc catcgccgcc
     4741 aaaccgtcca aaccgacggc acacgccgtc gcgccgtcgg catcgtagtc ggcgacaatc
     4801 aggatttttt ccttgcgccc aaccgcatcc gccaaacgtc cggcggcggc ttcgcagttt
     4861 gtcagcgttt gatacggcaa aagcgcagca agcttgtcgt ccaattcggc aggactttgc
     4921 acaccgcgcg aagcacaaag ccgggcgatt aaaggatcgg caccggcggt gagcaaatga
     4981 ttaaaaacgt cggtattgat ggatcgggtt tggattttga ctgacataac gtggggtttt
     5041 actgtgcgtg tgtgcttaaa atgccgtctg aaagcttttc agacggcatt gctgttcatt
     5101 tgcgggcggc atccacgacc gcacccagag ccgccgcagg caaagccagt accgcgcccg
     5161 ccgcatccag tatcaaaaag ggggggcgta tataagatat ttgcaaacag cttggatttg
     5221 tcggtatgtt tttcagtaac cgtgtaataa atatcggcag gcacactttg ctcaaaatga
     5281 taatcggcgt tcagtttttg cggcgtggcg tagtatttgc ctttggcaga gacgcagcgc
     5341 gtgtaaatag tctgtttgtc gagttcgacc gcttcaaact caagctgttt cagcttggcg
     5401 atgtcgtcag gcctgtcggt atcgtagcgc aggcaaaggc cgtcggtact gaaattctgg
     5461 ctgccgggcg attcgagttt gaccggcagg gcttggtggc gggcatagct cggggtatcc
     5521 tcaactattt ggaagggctt gtccagcccg gccttcaaaa tgcccgtcag cttcgccgaa
     5581 tcttcaggat tgacgacgaa ccagtatttt ccgcccatca tcaccaggct gcccttttcc
     5641 aattgggcat tgtcttcggc aaccacaccg aaggcgcgga tttggtcttt gtcaacgtgt
     5701 ttgcgggtga ttgtttcgct gaccgggttg ttcattcccc acaacatcaa cgtacagccg
     5761 ttcagcatca aggttgccgc caaaacggcg gcggtcgttt tacggaacaa cattttatcc
     5821 tccaatctaa ataacggcgg atgtcgtctg aaaatatgcc taaatttccg cgtccccgtt
     5881 ttccatcacg gttttcaact cttgaaacag ggcgcggaaa ttttttggtg gtttgttttg
     5941 ctcctgctct tttttggtat tgcggatgag tgtcctcagc ttgcccgcgt ccgcatgcgg
     6001 aaaatccgac ataaactgcg tcaacgcgcc gtcgtctgcc agcagctcca tccgcgtccg
     6061 ctccacacgt tgcaaaaagg cgttgtgcgc cgcatcgtcg ccgcgcagct tggcaagaaa
     6121 tgcctcgatg ggcgcgggat cggtatcgcg catcagtctg ccgataaatt gcgcctgccg
     6181 cttgagcgcg ccgttggatg tgattttttt ataggtaacg acggcttcgt acaaatcttc
     6241 gtccaagccg atttttttca acgtgtcgct tgagagcttg gtcagctcca tacccaaatc
     6301 ctgcaaaccg ttcatctgct ttttcatttg ggttttgctg acccattcgt cttcttgttc
     6361 aaacatcttc attctcaaat tttatccgac gggcgcattt taacagaatg attccgcgtt
     6421 cagacggcat tttgcaccgt ccgccctgca aacggcacgc ttttgcccct gccgcccgaa
     6481 acggttaaaa tgccgcaacg ctccaccaag aaagatttct atgctgttca accacaccgc
     6541 ttccgaactt ctcgacctct gccgccgcac gctcgacttg gcaaaagcag cgggcgcgac
     6601 cgccgccgaa gccgatttca gcgaatccat cggacaaagc gtcagcgtgc ggctgggcga
     6661 aatcgaacaa atcgagttcc agcaggacaa gtcgctggac attaccgttt acgtcggcaa
     6721 acgcaaaggc cgcgctggca ccgccgattt ctccgaacaa gccctgcgcg acaccgtccg
     6781 cgccgccgcc gacatcgccc gccacaccgc cgaagacggt tgcgccggac ttgccgaccc
     6841 cgagttgatg gcgcaacaca tcggcgaccc cgacctttac cacgaatggg atttggatac
     6901 ggaagccgcc gtcggctttg caaaacaatg cgaacaagcc gccctgaacg aggatgagcg
     6961 catcgaaaac tccgaaggcg cggcggtgca aaccggccat taccaatacg tttacggcaa
     7021 cacccacggt tttgccgcac accggcaagg cacgcaccac agcatttcct gcagcgtcgt
     7081 tgccgccgac gaaaacggga tgcagcgcga ctactggtac gattccgcct gccgccatcc
     7141 ggatatggac agccccgaaa ccatcggtca aaccgccgcc cgccgcactt tgcgccgact
     7201 cggcagccgc agcatcccga caggcagcta ccccgtcctc ttcgatacca ccgtttcggg
     7261 cggccttatc ggacacctcg tcggcgcact ctccggcggc gcgctctacc gccaaagcag
     7321 tttcctgatc gacagcatcg gcaaaaaagt cctgcccgat ttcctcaacc tgcgcgaaga
     7381 accgcacatc ccccgctctt tccgcagcag ctattttgat gcggaaggcg ttgccaccgc
     7441 accgcgcttc gtgattcaaa acggcattgt cgaaggctat ttcctcagca gttacagcgc
     7501 gcgcaaactc ggtatgcaga ccacgggcaa cgccggcggc gcgcacaacc tgtatttgaa
     7561 ccatacgcac gaaacgcaat ccgacctgct gaaagaaatg gggacgggat tattggttac
     7621 cgaactgatg ggacagggcg cgaacaccat taccggtgac tactcgcgcg gcgcggcggg
     7681 tttttgggtg gaaaacggcg tgattgccta ccccgtccac gagattaccg tagccggacg
     7741 cttgcaggat atgtaccgcg acattgtcgg cgtggcggat gacgctttgc ggcgttcgtc
     7801 caacaaaatc ggctcgattc tgattgcggg gatgacggtt gccggaagct gaacgccctg
     7861 ccgatgccgt ctgaagacga aggcagcacg ttttgccccg tgcctgcccg tttgccgttt
     7921 tatgccgcac cgccttttgc cgacaacatt gcacattttt tcatcagcac tgattttccc
     7981 ttatccgtca aaatactaag ccacttgtcc acaacagggt acacacaagt tcaggataag
     8041 gcgtgcgggg attggggttt ttccgccgcc ggcaaaacgg cagacaaaaa cggcgggaca
     8101 ttgcctgtcc cgccgttttc aaatatacat ttgaccgtac atcaggaatt aagcagcctg
     8161 aatgttggcg gcctgtttgc ctttagggcc ggtggttacg tcgaaagaga cgcgttggcc
     8221 ttctttcagg gttttgaaac cttccatatt gattgctgag aagtgagcga acaaatcttc
     8281 gccgccttca tcaggcgtga tgaaaccaaa acctttagcg tcgttaaacc attttacgat
     8341 accggttgcc attagaaact tcctatactc aaaaattaac aaaatcagca aaacaaggca
     8401 tacggcaaac cgagccgtct ggcgttcctt ccagcttgat atactcggcg gatgcccaat
     8461 cctgcttaac cgtcttttta catttgttaa gacaaaacgt caagctatgt tttcaaaaaa
     8521 gtgggaaaat aggcaaaagt accgaaaata caacaaaaat gccgtctgaa cgcggctttc
     8581 aaatgggaca cactatgacc gcgcaacacc aatccgacac gctcttgcac cgtctgaaca
     8641 ccctgccgcc gaaacgttac ggcgttttcc tattgaacga cgattacacc acgatggaat
     8701 ttgtcgtcga aatcctgacc gaaatcttta tgctcggaca agaacaggcg gtagcggtaa
     8761 tgctcttggt tcatcacgaa ggcaaaggcc tgtgcggcac ttacacgcgc gatattgccc
     8821 aaaccaaaca acaacaagtc atgcagcggg caaaagccga agggcatccg ctgcaatgta
     8881 ttgtcgagga gatttaatat gcttgcaccc gaattggaac agattttgca gcagctttac
     8941 cgcgaggcgc gtaaggctca ttatgaattt atcagcctcg agcatctgct tttggtactc
     9001 atcgaagaag atgcctccgt gccgaacgtc ttaaaactat gcggcgcgga tttgaaagtg
     9061 gtgtccgaac agctcgccgc cagcgttgcc gaaaacaccc ccctgattcc cgaacacctt
     9121 ttagacacgg tcgaaacacg gcccacgctc ggcttccaac gcgtgatgca acgggcgatg
     9181 gtgcataccc agtctgccgg aaaagccgca gtcgaaccgt tggacgtttt ggtcgcgctg
     9241 atgagcgaaa ccgacagcca caccgtctat ttcctcaagc tgcaatcggt tacgcgtttt
     9301 gaagttttgc gctgtattgc ccacggctct cccgatgaag atgaagatga agacgatggc
     9361 aactattctt cagacggcat ggacgacgat aatgaaaacc gcgccaaacc gggcaaaaac
     9421 cctttatcgg cgtacaccgt caacctcaac gccgaagtca aagccggccg tatcgaccct
     9481 ttgattggtc gcaaacacga aatggaacgg ctggtgcaaa tcctatgccg ccgccgcaaa
     9541 aacaatccgc ttttggtcgg cgaagcgggc gtgggcaaaa ccgcgctggc ggaaggtttg
     9601 gcacatcaaa tcgtcaacgg cggcattcca gacgcgctca aagatgccga agtgtacgcg
     9661 ctggatatgg gctcgctgtt ggcgggcacg aaataccgcg gcgactttga agcgcgggtc
     9721 aaatccgtct tgaaacagct cgaaaaaatc ccgcacgcca ttttgtttat cgacgaaatc
     9781 cacaccatca tcggcgcggg cagcaccagc ggcggcacca tggacgcgtc caacctgctc
     9841 aaacccgcgc tggcaaaagg ttcgctgcgc tgcatcggcg cgaccaccta cgacgaatac
     9901 cgcaccattt tcgacaaaga ccatgcctta agccgccgct tccaaaaaat cgacgtggtc
     9961 gaacccaccg tttccgaaac cgttcaaatc ctgcgcggct tgaaaccgat gtttgaagcc
    10021 ttccaccaag tccgctacac tcaaggcgca ctcgaagccg ccgccgaact ctccgcacgc
    10081 tacatcaacg agcgtttcct gcccgacaaa gccatcgacg taatggatga agcaggcgcg
    10141 gcgcaacgga ttctgcccaa atccaaacag aaaaaagtca tcggcaaagc acaaatcgaa
    10201 accgtcatcg ccaaagtttc gcggattccc gaaaaaaccg tgtcgcacga cgacaaacaa
    10261 gtgctgcaat tcctcggtcg cgatttgaaa aacatggttt acggtcaaga aaacgccatc
    10321 gacgcgctgg ttgccgccgt caaaatgtcg cgttccggcc ttgccctgcc cgacaaaccg
    10381 ataggcagct tcctcttctc cggcccgacc ggcgtgggca aaaccgaagc cgccaaacag
    10441 cttgcctact cgctgggcgt accgctgcaa cgctttgata tgtccgaata catggaacgc
    10501 catgccgtat cgcgcctcat cggcgcgcca ccgggctacg tcggctttga acaaggcggc
    10561 cttttgaccg aagccgtcaa caaacagccg cattgcgtat tgctcttgga cgaaatcgaa
    10621 aaagcccacc ccgacatttt caacgtcctc ctgcaagtca tggacgcagg caaactgacc
    10681 gacaacaacg gcaagagtgc cgatttccgc aacgtcatcc tgattatgac caccaacgca
    10741 ggcgcggaaa gcctcagccg ccccagcctc ggctttaccg ccaagcgcga acgcggcgac
    10801 gaaatgcagg caatcaacaa gctcttcacg cccgagttcc gcaaccgctt ggacgcgatt
    10861 atcccgtttg cacccttgtc cgaacccatc atcgtcaaag tcgtggacaa attcctgctc
    10921 cagctcgaac accagctcct cgacaaaaaa gtcgaagccg aattcacgtc ggcattgcac
    10981 aaatatctgg cggaaaaagg tttcgacccg caaatgggcg cgcgcccgat gcaccgcctg
    11041 attcaggaaa aaatccgcaa accgctcgcc gacgaactcc tgttcggcaa actatccgac
    11101 ggcggcttcg tgcggataga ctgggatgcg gcaaaagaag aagccgtgtt gaagtttaag
    11161 aaaagcaagg tcaaaataaa aaccgcctcc gcgtaaaatc aaaaatgccg tctgaaattt
    11221 cagacggcat tttctttcaa acaaaattac caatcgtgtt taagccaaat tttccagcat
    11281 ccatttgacg tagcggtcga cgcctgattt gacatcgaaa aattcttcct tatatccggc
    11341 ttcgcgcaat ttggtgatgt cggcttgggt gaagctctgg tatttgcctt tgagtgcgtc
    11401 ggggaagggg atgtagcgga taagttcttc ttccaccagc tctttcaagc tcatttcagg
    11461 cttgccttcg gcggcgcggc aggcgttgac ggtggcggcg gcgagttcgt taaactgttg
    11521 gctgcggccg gtaccgaggt tgtagatgcc ggaaagttcg ggatggtcga agaagtagag
    11581 gttgactttg gcaacgtctt cgacgctgac aaagtcgcgg gtttgttcgc cgttgccgta
    11641 accgtcattg ctgccgaaca ggttgacgta accgtgttcg cggtattggt ggaaatggtg
    11701 gaaggtgacg gatgccatgc ggcctttgtg ttgttcgtgt tgtccgtaaa cgttgaagta
    11761 gcggaagccg acgacttggg cggtgagacc ttctttcatg cggcggcgca atacttggtc
    11821 gaacaggaat ttggagtagc cgtacacatt aagcggtttt tcgagttcgc gctcttcgcg
    11881 gaagatttcg cctttgccgt aaaccgccgc actggaggca taaaggaagg gaatgcgttc
    11941 gtcctgacac cagttcaaca aatccagcgt gtactggtag ttgttttcca tcatatacaa
    12001 accgtcgtgg ttcatcgtat cggaacacgc gccttgatgg aaaacggctt cgatgttttg
    12061 ataaggtaaa atgtgttccc tcacttggcg gatgaattcg tgtttgtcga gataatgggc
    12121 gatttcgcac tcggcaaggt ttttgaattt ttcgcctttg ctcaaattat cgacggcgac
    12181 gatgtcggta ataccgcgtt gattaagtgc tttgacgatg ttgctgccga taaagccggc
    12241 cgcgcctgtt acgatgatgg tcatattcgg tttcctttgt tttgagtaaa attaataaac
    12301 ttagttacaa gaaatcatcg atattgtcct gaaacacaaa tttgcgatga tattccaaat
    12361 atttttctct tccttcggga tatattctca tcttattttt tgcatttgga ttgatattaa
    12421 gtttttccca tgtgtaacgg cttagtcgag taccgcaaat caaacgacca tcatccaaga
    12481 aagtaatata accttgatcg aacaaccaat cataagtcgg cgttaaagct aacccattta
    12541 aataatctaa tgcctctttc tcatttttct cagtaataca aaccatataa ggtttaatat
    12601 gactagccct taacaaaacc tcatctgtaa ttaatgtaaa tggacattgc ggcatatgat
    12661 ttataacatc cttgcgatat ttctgctgcc cttttctact tttctgttta atgatttctt
    12721 gctctacgaa ttgattaaat tctgaagccg gtatttctaa tttttctctt gataagagtt
    12781 gcgggtgcac gatagagcga aattgataat ccaaaaatat tcggaaataa aataatggtt
    12841 ctgaatcttc tatatctttg acaggcaata gtttaagaat tgataaataa ctaattttgg
    12901 gcaatacaat cttacgccaa attgaccata tatcatcctc cgaacggata taacccctac
    12961 ggtaaccttt agaattttcc aaatggtctg atacatcatg gattgaaaag aaaatatttt
    13021 ctgtaggcag attattaata cttaaaacgt aatcatcata aaaccttgaa atatcatgat
    13081 gatatttctg ttcttgtttg tgatattcaa cttttgcatc aaccaaataa cgctgtaaat
    13141 ttttcttatt aaaaaaacaa tttttctgaa tgagaggata ttctaccctc cttccatttt
    13201 ttgtctcagt aatcccccaa ttttcatact caaaaaatgt acttagtttt cgaccactat
    13261 catcaatata actacctaca tatttgcgtg cttccccatt accaccaaaa acagataact
    13321 tgttattcct gtgaataaaa ctatcttccg cacgtaaatt ttgaatacta tcgacaataa
    13381 aatactctat gttttcaata gtaattcttt gaaaatccgg cattttactc tccataaagc
    13441 gtaatatcca tttgttttaa tatagcgatt aatacatcaa caacaatact gtttcccgct
    13501 tggcgataca tttgagtatc actaactaaa attttaaaat catctctaaa acccattagc
    13561 cgcaaacatt ccctaggtgt taatttacga atacgtccac gattatgagt aacataatta
    13621 tcaaccccgg cacgatgcat tttatgcata gtttgcaata atgggcgagc tacaggcaaa
    13681 tcagtttccg tactggtttt aaaatttttt gttcctcctg ccaaaacata atttttgatt
    13741 ttttcagaaa gataatattt ttcctcaacg ttatttacat caaaaataaa gtcatcaaat
    13801 tcagattcgc gggctgcctg aaaaataaaa tcaccatgcc aattaaattg ttgatttgct
    13861 ttttggcata aggcaatttc tccattaatc tgtgtataac gtttttgtct atttttagaa
    13921 ctggtaacaa atttcacacc cttttcacgt aaaaaatatt tgctatcagt atagtcctcc
    13981 aaaaaatctt gcatagtatg ttctaattca atcttttcag gaaactgaaa accatttata
    14041 ggaggggtat gaaagccaac aacaaaaata cgctcacgat gttgaggaat cccataatcc
    14101 ttactattca ttatttggaa atataagtca taaccaagtg aataaaaaac actttttaca
    14161 actttccaag tttttccatt atcatgattg agcaagccct ttacattttc ataaataaat
    14221 atctttggtt ggacttcatc cacaacgcga gaaaattcat agaataatgt tcctcgtgta
    14281 tcttctaatc ctgcacgttt gccaaccatg gaaaatgctt ggcaaggact gcctccaact
    14341 aaaatatcaa cttgatttct aaactttctc gcatcaaatt gagtaatgtc gttatgccaa
    14401 aaatcttcat ttaatttata gtttccaaga taactttttt taacgtatgg atcaatatct
    14461 cctgcaaaaa caatggtatg gtttaaattt aatcggtgga atgcctgttc aaccgcacca
    14521 atcccgctga atacggttgc taatctaata tgtgaatcag gtttaagaaa agttttagat
    14581 ttccaacctt gttgactggg aaagagcaaa gttttttgta atcgagtatc gtgtgtctgt
    14641 gccattgtcg aaatagtcat acttatatcg ttctgtttat cttatcaata tgaaaactac
    14701 attgttgatt gccctgacaa tgccttgatc aattcggcaa acgagcaaac cgccgtaccg
    14761 agtttcgcca cgacaacccc ggccgcagta ttggcaaggt acatggcttc gggcatggtg
    14821 cagcctgccg ccaaacccaa gcccattccg gcaatgacgg tgtcacccgc accggatacg
    14881 tcgtaaactt cttgggcgcg ggtgggctgg taaatcggtt cgccttcgct gaacaaggtc
    14941 atgccttctt cgcttcgggt cagtaaaacg gcggtcaggt cgaggtggcg gcgcaggttt
    15001 tgcgcttttt cggtcagctc gctttcgttt ttccaactgc cgaccacttc tttcaattcg
    15061 gcgcggttag gcgtaatcag agttgcaccg acatattttt cgtaatcgtc gcctttgggg
    15121 tcgattaaga cggttttgcc ggcgtgtttc gcccaatcga tcatatcgga gatatgcgac
    15181 aggccgcctt tgccgtagtc tgaaaaaatg attgcgtcgt attcgggcaa gatttcgcgg
    15241 tatttctgct tgatttgttc caacacttcg cagttgggat gttcttcaaa atcaagacgg
    15301 ataagctgct ggttgcgggc gacgacgcgc agtttgacgg tggtggcgat ttgtttgtcg
    15361 cgcatcagat aggaggcgac gccgtcctgc accatcagcg catcgagcgc gtcggcggct
    15421 tcgtcgttgc cggttacgga caacagccct gccctgccgc ccaacgaagc gatgttgcgc
    15481 gcgacatttg ccgctccgcc cgcgcgttgg tcgattcgtc cgattttcgc caccggcacg
    15541 ggggcttcgg gcgaaatacg ggacacatcg ccgaaccaat agcggtcgag catcacgtcg
    15601 ccgacaacca ggactttggc ttgcgcgaaa cgggatttga gggtttcttg ttggaacttg
    15661 gcggacatat tgcctccgtt gtattttttc agacggcctc agcctgtaag gccgtctgaa
    15721 aatcagtttg cttcaaggtt tgccacgcgg ttcacttcgc gcaatacggc taccggatcg
    15781 gcagcttggg ttacaggacg acccattacc aaataagtcg aaccggcagc caaggcttcg
    15841 gccggtgtca tgatgcggcg ctggtcatca ttattgccgg caacgtccaa gcggatgccg
    15901 ggcgtgacca agacaaaatc ctgtcccaat tcgcggcgca gcggcgcggc ttcttgggcg
    15961 gaacagacca cgccgtccaa gcccgaactt tgcgccagtt ttgccaagcg gatgacttgt
    16021 tcttcagggg cggtgttcaa accgatttcc gccaaatcgc tttgttccat gctggtcaac
    16081 acggttacgc cgattaagag cggcttcgtg ccgtatccgg caacggcttc tgctgcggct
    16141 tccatcatac ggcggccgcc cgatgcgtgc atatcgacca tccaaacgcc catatcggca
    16201 gcgactttgc aggcttgcgc gacggtgtgg ggaatatcgt ggtatttcaa atcgaggaaa
    16261 agtttgaaac cttgattgat taagctttct gccaaattgc gtcccgtcgc ggtaaacagc
    16321 tctttgccga ttttgatttg acacaatgtc gggtcaaggt tgcggacgaa tccgagcgtg
    16381 tctttttcgt tggaaaaatc aagggcgacg ataacggggg tgcgttgttg cggagtttgg
    16441 aagtcggaga ttaaaggatt catggtgttt tcgtttcggg ttttcagacg acctttatcg
    16501 tctttttcgc agtatagtgg attaacttta aaccggtacg gcgttgtctc gccttagttc
    16561 aaagagaacg cttctctaag gtgctgaagc acaaagtgaa tcggttccgt actatctgta
    16621 ctgcctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataac aacgtaaatt
    16681 caaaccgtag gtcgggcatt gatgtccgac ctacgcgaac tcaagcctcc gacaaactgt
    16741 ttcggataaa tttgaaccgg tacatcagcc gtcattcccg cgcaggcggg aatccattga
    16801 taaaactcag ttcatcagat ttaaacagcg attgcccaaa ttcagaaatg gattcccgcc
    16861 tgcgcgggaa tgacgccaaa tgattatttt tcggttgcaa cacgctgttt atcaaaaaca
    16921 ggtcgtctga aaacctgatt ttctgttttc agagtgcgaa gcctgcccgt gggtacgacc
    16981 ttttcgattt atttgcccaa tgcgcccaat acttcggctt tcacggcttc taccggctga
    17041 gtgccgtcaa ctttgatgta tttaggcgcg tgttcgcctt ccagtttgct gtaaaaatcg
    17101 accaaaactt cggtttgctc gtggtaaacg gcaaggcgtt ttttcacggt ttcttctttg
    17161 tcgtcgtcgc gctgaatcaa atcttcgccg gttacgtcgt ctttgccttc aactttgggc
    17221 gggttgtagg taacgtggta agtacggccg gaagccaaat gcacgcggcg gccgctcata
    17281 cggtcgacaa tcacgctgtc aggcacgtcg atttcaacga ctgcatccaa atccacgcct
    17341 gcttcaacca tcgcttcggc ttgtgccaat gtgcgcggga aaccgtcgaa caggaaaccg
    17401 tttttgcagt cgtcttgcgc gatgcgttct ttgaccatgc cgataatgat gtcgtcgcgc
    17461 accaagccgc cttcgtcaat gatttttttc gcttccaaac ccaagggcgt gccagcctta
    17521 atcgcggcgc ggagcatgtc gccggtagag atttgcggaa tgccgaacgc ggcggtgatg
    17581 aattgcgcct gagtgccttt gcccgcgccc ggcgcgccta aaagtaatgc tttcatgaag
    17641 tgatcctttg aaatgtgttt attggaatca aacagatggt ttacgattct acacggcttg
    17701 gcacaatagt tcaaaaacca actgcacaaa ctgccatttg caagcaaatg ttttgcatac
    17761 ctttgccgag tctgatattc acaagccggc aactttttgg gacaatcccc aaaatttcaa
    17821 aacatcacac cctaccaaaa aggaacatcc gtgaaacgaa tttttctgtt tttggctacc
    17881 aatatcgctg ttttggtcgt aatcaacatt gttttggcgg ttttgggcat caacagccgg
    17941 ggcggcacgg gcagcctgtt ggcgtattcc gccgtcgtcg gcttcactgg ttcgattatt
    18001 tcgctgctga tgtccaaatt catcgccaaa caatcggtcg gcgcggaagt tatcgacacg
    18061 ccgcgcaccg aagaagaagc ctggcttttg aacactgtcg aagcccaagc gcggcaatgg
    18121 aacctgaaaa cgcccgaagt cgccatctac cactcccccg aacccaatgc ctttgccacg
    18181 ggcgcatcga gaaacagctc cctgatcgcc gtcagcaccg gtttgctcga ccatatgacg
    18241 cgcgacgaag tggaagccgt attggcgcac gaaatggcac acgtcggcaa cggcgatatg
    18301 gttacgctga cgctgattca aggcgtggtc aataccttcg tcgtgttcct gtcgcgcatt
    18361 attgccaacc tgattgcccg aaacaacgac ggcagccagt cccagggaac ttatttcctg
    18421 gtcagcatgg tattccaaat cctattcggc ttccttgcca gcttaattgt catgtggttc
    18481 agccgacaac gcgaataccg cgccgacgcg ggcgcggcaa aactggtcgg cgcaccgaaa
    18541 atgatttccg ccctgcaaag gcttaaaggc aacccggtcg atttgcccga agaaatgaac
    18601 gcaatgggca tcgccggaga tacgcgcgac tccctgctca gcacccaccc ttcgctggac
    18661 aaccgaatcg cccgcctcaa atcgctctga gccgatttga aacaacaaaa aaaccgtacg
    18721 cgcaagcagt acggtttttt gtatatgctg tctgaaaaca tcaggtttcc ataccaacac
    18781 caaccgtatc catttccgca ccggatacgc cgcattgata taccctgata aaccgtgtcc
    18841 ggaca
//
LOCUS       DAWFAD010000028        18666 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWFAD010000028 DAWFAD010000000
VERSION     DAWFAD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18666
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.028"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            54..1064
                     /gene="waaF"
                     /locus_tag="WGO49_000711"
     CDS             54..1064
                     /gene="waaF"
                     /locus_tag="WGO49_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ0305846.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLDIGKPVLAFCPGAEFGPAKRWPTRYFAELGKHYSAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            1119..1565
                     /gene="smpB"
                     /locus_tag="WGO49_000712"
     CDS             1119..1565
                     /gene="smpB"
                     /locus_tag="WGO49_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ0305847.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            1996..3981
                     /locus_tag="WGO49_000713"
     CDS             1996..3981
                     /locus_tag="WGO49_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ0305848.1"
                     /translation="MISLFFSILYTSPLLAVDYVYDNSKLTNEQIDRLKKLRDKSSEY
                     WKQETYLLKPTNGSSQNSLKVPALYSKTHQFKNINNSKKISFYDKEYTEDYLVGFARG
                     FGVEKRNGEEEKTLQQYFKDCVNTGNSNNDKCEQPSFENSGPRLIKSDIFALAPQIKN
                     SHINSEILSVGNYIEWLRPTLNKLPSWQEHLYSGLDPFHYIEVTDNSHVIGQTISLDE
                     FRLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGARFRFAYDLKDKVS
                     ETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNGTADKHAFRLSGKHQKGIYT
                     LSLQQRPEGFFTKVQERDDISIYAQQAQAANTLFALRLNDKNSDIFDRTLPRKGLWLR
                     VIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSVGLMSGQAEQRSTFRNP
                     DTDNLTTGNVKGFGAGIYATWHQLQDKQTGAYADSWVQYQRFRHRINTEDATERFTSK
                     GITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSENAHVNLLGSR
                     QLQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEIDGERRVINNKTAIESQL
                     GVAVKIKSHLTLQASFNRQTSKYHQAKQGALNLQWTF"
     gene            complement(4162..5529)
                     /locus_tag="WGO49_000714"
     CDS             complement(4162..5529)
                     /locus_tag="WGO49_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0305849.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLIIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(5639..5920)
                     /locus_tag="WGO49_000715"
     CDS             complement(5639..5920)
                     /locus_tag="WGO49_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305850.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAAAT
                     EAPAAEAPTAEAAATEAPAAEAPAAEAAATEAPAAEAPAAEAPAAEAAK"
     gene            6393..6875
                     /locus_tag="WGO49_000716"
     CDS             6393..6875
                     /locus_tag="WGO49_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0305851.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            6962..7774
                     /gene="hpnC"
                     /locus_tag="WGO49_000717"
     CDS             6962..7774
                     /gene="hpnC"
                     /locus_tag="WGO49_000717"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ0305852.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(7845..8315)
                     /locus_tag="WGO49_000718"
     CDS             complement(7845..8315)
                     /locus_tag="WGO49_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ0305853.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHKIA"
     gene            8406..10247
                     /locus_tag="WGO49_000719"
     CDS             8406..10247
                     /locus_tag="WGO49_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ0305854.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTNPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEVGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANKP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            complement(10763..11959)
                     /locus_tag="WGO49_000720"
     CDS             complement(10763..11959)
                     /locus_tag="WGO49_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0305855.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPNHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            12310..12609
                     /locus_tag="WGO49_000721"
     CDS             12310..12609
                     /locus_tag="WGO49_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305856.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            12609..12794
                     /gene="ccoS"
                     /locus_tag="WGO49_000722"
     CDS             12609..12794
                     /gene="ccoS"
                     /locus_tag="WGO49_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ0305857.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            12784..14094
                     /locus_tag="WGO49_000723"
     CDS             12784..14094
                     /locus_tag="WGO49_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0305858.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRYKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            14252..14986
                     /gene="dnaQ"
                     /locus_tag="WGO49_000724"
     CDS             14252..14986
                     /gene="dnaQ"
                     /locus_tag="WGO49_000724"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ0305859.1"
                     /translation="MIQRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGAAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            14983..15672
                     /locus_tag="WGO49_000725"
     CDS             14983..15672
                     /locus_tag="WGO49_000725"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ0305860.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTLGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLETGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIHPLLVQGDARNLKLTQPQD
                     AYIVRLLLDAV"
     gene            15704..16186
                     /locus_tag="WGO49_000726"
     CDS             15704..16186
                     /locus_tag="WGO49_000726"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ0305861.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            16263..16934
                     /gene="rpiA"
                     /locus_tag="WGO49_000727"
     CDS             16263..16934
                     /gene="rpiA"
                     /locus_tag="WGO49_000727"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ0305862.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLMMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            17003..17674
                     /locus_tag="WGO49_000728"
     CDS             17003..17674
                     /locus_tag="WGO49_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ0305863.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            17853..18599
                     /locus_tag="WGO49_000729"
     CDS             17853..18599
                     /locus_tag="WGO49_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0305864.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
BASE COUNT         4446 a         4249 c         5307 g         4664 t
ORIGIN      
        1 ttttccaacg gtttcgcggt aaaattctgc ctgtctgatt tcggtttctt gttatgtcca
       61 tcaaaatcct gattatttct cccagttgga taggcgactg cgtgatgacc cagcccttgt
      121 tccgccgttt gaaggaactt caccccggtt gcacgattga tgtgttcgca ccgaagtggt
      181 cgatggcggt gtttgagcgt atgccggaag tgaatgagat tcttgaaaat ccgttcggac
      241 acggtgcgtt ggagttgaaa cgccgctggc gggtcggtag ggatttgggg cggcgcggat
      301 acgatcaggt tatcgtgttg cccggttctt tgaaatcggc aatcatcgcg ctggcgacag
      361 gtatcggtaa aaggacgggt tatgtcggtg aaagccgtta ttttctgttg aacgatatac
      421 gcaggctgga taaggaacgt ctgcctttga tggtggacag atatacggct ctcgcgcatc
      481 cgagccagga ggattttgac gggcattcgg gattccccga gttttccatt gatgaacggc
      541 ggcgggaaat ttctgtcgaa acctttggct tggatattgg aaagcctgtt ctggcttttt
      601 gtccgggtgc ggaattcggg ccggcaaagc gttggccgac aaggtatttt gccgagttgg
      661 gcaaacatta ttcggcggcg ggttggcagg tttggctgtt cggttcgcaa aaagatgatg
      721 aaattgccga ggaaatcaac tgcctttcag acggtatgtg tgtgaattta tgcggaaaga
      781 ccgatttgtc gcaggcaatg gatttgctgt cgttggcgga cacggtcgtg tgcaacgaca
      841 gcgggctgat gcacttggcg gcggcattgg gcaggaaggt ggtggcggtt tacggttctt
      901 ccagcccgac gcatacgccg cctttgagcg acagggcgaa aatcgtcagc ctgcacttgg
      961 aatgttcgcc ctgttttaag cgggaatgtc cgctggggca taccgactgc ctcaacaggc
     1021 tgtatcccga gaagattgtg caggcggttg aagaggcggt atgatgggtt ctgttttcgg
     1081 gtttatgccg tctgacggcg gatattgata ggatgtttat ggctattgcg aacaataaaa
     1141 aagcatttca cgattttttc attgaagacc ggattgaagc cggtttggtc ttggaaggct
     1201 gggaagtcaa agcaatccgc gccgcgcgcg tacagcttaa agagagttat atctattgga
     1261 aaaaagacgc gttttatctg gtcggctgcc atattacggc tttgcccaca gcctcgacac
     1321 acattaaacc agatgccgta cgtccgcgca agctcttgtt gaaccagtcg gaaatcaaca
     1381 aattaatcgg caaaaccgaa cgtgccggct acaccatcgt gccgctggat ttgcattttt
     1441 cgcgcggaaa aatcaagatg gaaatcggtt tggcaaaagg taagaaacag cacgacaaac
     1501 gtcagagtat gaaagaggcg gattggaaac gcgagaaaca gcggttgatt aagcatacgc
     1561 gctgattgtg gaaacagtgc ttcagagggt atgggcaagg ctgccgcctg atgtgtaaac
     1621 gttctacacc taaaccagca tccgctttaa tcagcacagc atgatgcccc aaaccttcgt
     1681 caaaggtttg agcaatttga tgataaattg gcattagcca agggtaaagt gcggtcattt
     1741 ttcctgaaat ttttgtaaat aacgagggca attatacaca ttttcaaact ggcttgacaa
     1801 aatagcaaaa agcaaacgtt tacctttaat aaataagaaa taaatagaga aaaatgtgag
     1861 ctaaatgaca ttttttgttc agttttatgc taaaatccac gccccattta tttttacttg
     1921 acaaaggaaa atttattatg aaactcgaag caagcaagca agcaagcaag caagcagaag
     1981 tttaaaaaat catttattat aagtctattt ttttctattc tttatacctc tccgcttttg
     2041 gctgttgatt acgtttacga caattctaaa ttaacaaatg agcaaattga tcgcttaaaa
     2101 aaacttagag ataaaagcag cgaatactgg aaacaagaaa cttatcttct aaaaccgacg
     2161 aatggtagct ctcagaactc tctcaaagtt cccgcactat actctaaaac acaccaattc
     2221 aaaaacatca acaactcaaa aaagatttct ttttatgaca aggaatacac tgaagattac
     2281 cttgttggct tcgcccgagg tttcggggtt gaaaaaagaa atggggaaga agaaaaaaca
     2341 ctccagcaat attttaagga ctgtgtaaat actgggaatt ccaataatga taagtgtgaa
     2401 caaccatcct tcgaaaactc tggcccaaga ttaataaaga gcgatatttt cgccttagct
     2461 ccacaaataa aaaatagcca tatcaacagc gaaattttat ctgtaggtaa ttatatcgaa
     2521 tggcttcgcc caacacttaa taagcttcct agttggcaag agcatctcta ttctggttta
     2581 gatccattcc actatattga ggttacagac aactcacacg ttatcggaca aacaattagt
     2641 ttggatgaat tccgcttaga aaattctctc tgggaacccc gttgggattc tgatgttggg
     2701 gaattaaaaa caacgaatgc agacatccgt tttaatacta aaagtgaaag tttactcgta
     2761 aaagaagatt atgctggcgg agctcgtttt cgttttgctt acgatctaaa agataaagtc
     2821 tctgaaacac cagttttaac ttttgaaaaa aatataactg gcacttccga catcatcttc
     2881 gaaaatccaa tagacgattt aaaaagtctc gacgggcatc aaattatcaa agtaaatggc
     2941 acagcagata aacacgcatt ccgtctttct ggcaaacacc aaaagggaat ttatacgctt
     3001 tctttacaac aacgcccaga aggctttttt accaaagtgc aagaacgcga tgatatttcg
     3061 atttatgcac aacaggctca agccgccaat accttattcg ccttgcgttt gaacgacaaa
     3121 aacagcgata tttttgaccg cactttacca cgcaaaggct tgtggttgcg tgtgattgac
     3181 ggacattcca accaatgggt gcaaggcaaa acagcacctg tagaaggcta tcgtaaaggt
     3241 gtgcaactcg gtggcgaagt attcacatgg caaaacgaaa gtaatcaact ttccgttggc
     3301 ttaatgagcg gacaagcaga acaacgcagc actttccgca atccagatac agacaatctt
     3361 acaacgggaa atgtgaaagg ctttggggcg ggcatttatg ccacttggca tcagcttcag
     3421 gacaaacaga caggtgcgta tgcggatagc tgggtacaat atcaacgctt ccgccaccgc
     3481 attaacactg aagatgctac agaacgtttt acttcaaaag gtattaccgc ctcaattgaa
     3541 gcgggttaca atgctttatt ggcagaacac ttcactaaaa agggcaacag ccttcgtgtt
     3601 tacctacaac cacaggcaca attgacttat ttgggggtaa acggaaaatt cagcgatagc
     3661 gaaaatgccc acgtgaattt acttggctcc cgccaattac aaactcgggt tggcgttcaa
     3721 gctaaagctc aattttcact ctataaaaat atcgctattg aacctttcgc cgctgtgaac
     3781 gcgctttacc acaacaaacc tttcggtgtg gaaatagacg gagagcgtcg agtgataaac
     3841 aataagacgg caattgagag ccaattaggt gtagcagtga aaatcaaatc tcacttaact
     3901 ttacaagcct cattcaaccg ccaaacaagc aaatatcatc aggctaaaca aggtgcgttg
     3961 aatttgcagt ggacgtttta agttaagttg taacaaaata aaatcggcag ggaaattcac
     4021 tgccgatttt tttattttaa aaatgcggtc atttttcgtg tcatttccac caatttttta
     4081 ccgcactcac gagctcgcgc taaaccgaca atcggatcta ttgtcccttt ctttgttgtg
     4141 tatgcaaatc agaaatgccg gttacggaag cagtttgccc ggattcataa tgttatatgg
     4201 atcaaggtgt tgtttgatgc ttttcatcag ggcgatttcg gcaggcgtgc gtactttgtc
     4261 cagccactgt tttttgatga tacctatgcc gtgttcggcg gcaatcgtgc cgttgcaggc
     4321 aagaacgttg cgataaactg cgtcgttgat gtcgttttcg taacgataga cttcattgct
     4381 gaggatttcg ggcaggaaag tattgtagtg caggctgccg tcgccaagat gtccgaagca
     4441 gacgatttgt atgcctttaa aattctgttc caaatctttg gcgcaccggc ggacaaagtc
     4501 ggcaacgcgt ccgataggaa cggcaatatc gtgtttgatg ctggtgccca gtttgcgttg
     4561 ggaggcggag atgttttcgc gcaatgccca catatggata cgttcttgtt cgctttgtgc
     4621 caatacgctg tcggtaaatc cttttttata aagaaattcg acgagccgat catcaagatt
     4681 gctgtcgggt aatgagtcgg tcaactcaag taaaatatgc cattctgaat gtgtcggcag
     4741 ggggaatttg ctgaattcgg aagacaactc ggcggcaaaa cggccgatca gctcaaaact
     4801 gcataggcgt tcggcaaagt gagcttgggt ttcggtcagc aggcggacgg cggattcgat
     4861 gtcgggtatg ccgacccatg ccgttgcctt gtccgaggga cgggcaaaaa gcttgagtgt
     4921 ggcggcagtg atgatgccca gtgtgccttc gctgccgata aacagatggc gcaggtcgta
     4981 gccggtggtg tttttatgca ggggatggag atgggaaacc agctcgccgt tggggaggac
     5041 gacttccaaa ccgataatca ggtcgcgcat cgtgccgtaa cgcaatacgt tcaaacctcc
     5101 ggcattgcag gcgatgttgc cgccgatttg acaggagcct tcgctggcga gactgagtgg
     5161 gaacagcctg tttgaggctt cggctgcctg ttggacggtt tggagtacgg aacctgcttc
     5221 gacggttatg cagttgtctg acaaattgat gctgcggatg cggttgagtt tggaaaggtt
     5281 cagcaatacg ccgttttccg atactgccgc gccgcacaaa ccggtattgc cgccttgcgg
     5341 cgtaaccgga atacggtgtt cgtaacaaaa acgcataatg gtttgcacgc tttcaacgct
     5401 gcgcggctgc aaaatgatgt ctggtgcaga cgtaaagcgg ttgcgctggt cttttaaaag
     5461 cgtcggagaa agttcggcaa tttcatctgc cggcaggagt cgggaaaatt cggtatgaag
     5521 gtcaggcatg tttgtcggaa gggtagggag aagattgagg aagggaatct gatgccgtct
     5581 gaaaaatcaa aaaagcagga tactgaacgt atcctgcttt tttgcaagcg gaaaatgctt
     5641 attttgcagc ttcggcagca ggagcttcgg cagcaggagc ttcagcggca ggtgcttcgg
     5701 tagcggcagc ttcagcagcg ggagcttcgg cggcaggtgc ttcagtagcg gcagcttcag
     5761 cagtgggagc ttcggcggca ggtgcttcag tagcggcagc ttcagcagcg ggagcttcgg
     5821 cggcaggtgc ttcggtagcg ggagcttcag cggctttttc accgccgcag gctgccagga
     5881 ccaaagacaa caaagcagcg gcaaacaggg attttttcat tttagtaacc tttagaacca
     5941 atttaaatta ttgaaacaaa taggtcttta tcgaccatac tacaaaccgg gttggaaatt
     6001 catcgctgaa tttgcgtaat tatgcactat ggaataaggg attgcaatgt cgtacagatg
     6061 ggaaaaaggt ttgaatgaaa agttttctgg cgggcggatt gataaatcct gtaaaacggt
     6121 tttgctgtta aaaattcgat agggataaat attatagaga ttaatataat ttcagtatct
     6181 tgtatgattt attaaggcgg attgggaaag tcggcagaca ggtcggaatg gttttaagaa
     6241 tgttgtaaaa aagttacacc gcaataaaat aacaaaaatt tacattgaaa tatgtgctgt
     6301 acagtttatg aacaggttaa cggtaatcgg ccggcgcggc ggttgtgtta gaatgcgcct
     6361 gtaatcaatc tgctgttcct gaaaggacaa atatggctat cgtgaaaaat tccgtggttt
     6421 cgctgcatta tgagatgtat gatgccaaca atcagctttt ggacaaaacc gaagaaccga
     6481 ttgcgtatct gcacggcggt tacgacggca ttttcccttt ggtggaagag gcgttgcacg
     6541 gtaaggatgc cggcgatacg gtcgatgtgg cgctgtcgcc cgacgatgcg ttcggcgagc
     6601 aggatccgga gttggtccgt attgaagatg cgggcgtgtt ccctgttgaa gtcgaagtcg
     6661 gcatgatgtt tgaagccgac gatcctgaaa ccggcgatgt tgtcgtctat cgtgtaaccg
     6721 atgttgccga cggcaaggcg gtggtggacg gcaaccatcc tttggcaggc atgaaaatcc
     6781 gctttaaggc tacggttgaa agcgtgcgcg atgcatccga tgaggaaatc gcacacggtc
     6841 atgtccacgg cccgcacggt catcaccacc actgattgcc ggcagcttgg aggtatctga
     6901 tgccgtctga aaagggtttg ttccgtttca gacggcattt tgtttgatgg atggagaagg
     6961 tatgtcggtt gggcattatg agaattttcc cgtcggttcg ctgattttgc cgcgcaggtt
     7021 gaggaagccg gttcacgcgg tgtacgcgtt tgcacggacg gcggacgata tggcggacga
     7081 gggcagtatg ccgtctgaag ccaggttggc ggggttggag ggtttgcagc gcgagttgga
     7141 cgtgttggca tcgggcggcc ggtcggcgca tcctttgatt gcacgattgg atgccgaggc
     7201 ggttgtgccg ttcggtttgg atttgcagcc gttttatgat ttgctttctg cattttctca
     7261 ggatgtggtt aaaacgcggt acgcgcattt cggcgagctg gccgattatt gtcggcgttc
     7321 cgccaaccct gtcggacgca ttatgctggc tttgtacggg aaaacggatg cggtgtgcgt
     7381 ggcgcaaagc gacggtatct gtacggcttt gcaactggtg aatttttggc aggatgtggc
     7441 tgtggattgg caaaagggca gggtttatat cccgcaggac gatttgttga aattcggtgt
     7501 ttccgaagga cagattgcgg agggtagggc ggatgcggcg tttcagcggc tgatggcgta
     7561 tgagtgccgg cgcgcattcc gtatgctgaa ggcgggttcg cctttggcgc gcgaattgaa
     7621 agggcgtatc ggtttggaac tccgtatgat tgtgttgggg gcgcagttga ttttgcagaa
     7681 actggacgcg tgccgatacg atgtgtttgc acaacgtccg gttttggata agaaggattg
     7741 gttgattatg ttgaaacgcg cgttgtggaa atgatggggg aaatgaatgc cgtctgaaag
     7801 gggttgttcc gcttcagacg gcattttgca ggcggttggt atggtcaggc gattttatgc
     7861 gggttcaacc tggcttgatc ttctgcaccc aagccgatga gtttgcgcag tcgggtcatg
     7921 tagaaattga catccaaacc ggtgccgtag cgttgcgccg tccacagtgt ttccgccagt
     7981 gcctccatca tttcgtgttc ggcttccagc cagccgcgtt tggcgcacag ggtgtcgtgg
     8041 attgcgcgta tgccgtgcgg ctggtctatg cccgcctgtt cttggacgga caaatgcagc
     8101 gacatatgca ggaaggggtt gctttcgccg ttttcgggca gccagtcggt gtccagatgg
     8161 tcttcgatgc gttcgagata acggtggtat tcgggatggg cttcgataat gcggagggct
     8221 ttctgttcca gtgcgctcag ttgcagcgga ttgagccgct gctgccacac gcgggcgaaa
     8281 aagcggcgga catcgtgggt attgacatcg tacatggcgg gttcggtaag atgtttatgg
     8341 aaataggttc ggataatggc gggttttaca taaattaaat cccaccgtca accttaccat
     8401 ttataatgca gacaaattat ttgtctgaaa cggattcgat tatgaaaaaa ctgatttgcc
     8461 tgttcgccgt atttttgatg ttgtgcggac gagctttcgc gctggatgcg aacgatctgc
     8521 tgccgccgga aaaggcattc gtgccggagc ttgccgttgc cgacgacggt gtgaacgtcc
     8581 gtttcaggat tgccgacgga tactatatgt atcaggcgaa aatcgtcggc aagaccaatc
     8641 cggcggattt gttgggacag ccttctttca gcaagggcga agagaaggaa gacgagtttt
     8701 tcggcaggca gacggtttac catcacgaag cgcaggttgc ctttccttat gcaaaggctg
     8761 tcggcgaacc gtataaattg gttttgacct atcagggctg tgccgaagtc ggcgtgtgct
     8821 atccgcccgt ggataccgag tttgatattt ccggcaacgg cacttaccat ccgcaaaccg
     8881 acgaaccggc atccgccaaa gaccgctttt tgcagccttc ctctcaaaac ggcagcgggg
     8941 cgctgccgcc cccgaagggg gatgagggcg gcgacagccg tttcaagctg tcttgggata
     9001 cgctcaacgc caatcttttg gcgttttttc tcgccggttt gggtctgagt tttaccgcct
     9061 gtatgtatcc cctgttgccg attgtttcca gtattgtggt cggcgacaaa aaggcgggca
     9121 aggcgcgggc gtttgtgctg tccgtcgttt atgttcaggg tttggctctg acttatacgc
     9181 tggtcggcat tgttgccgga ctgacgggcg cgctgctgac cgtatggttg cagcaggctt
     9241 gggtcgtgtt ggcggcatcg gctttgatgg tcgtcttggc actgtctatg ttcgggctgt
     9301 tcaacatcca gcttcccaac gccgtgcagt cgtattttca gaatcaaagc agcaggcttt
     9361 caggcggtaa aatcgtttcc gtctttatta tgggcatatt gtccgcgctg attgtcgggc
     9421 cgtgcgtcgc cccgccgctg gcatttgctt tgggctacat cggtcagacg ggcgatgcgg
     9481 ttttaggcgg tttggcactt tacactttgg cgttgggcac cggcgttccg ctgattgcca
     9541 tcggcacgtt cggcgggcat atcctgccta aggcaggcga ttggatgaat gccgtcaaat
     9601 acgctttcgg ctttatcctg cttgccgtcg ccgtttacct cgccacgccg cacttgccct
     9661 attatctcgt cgtcgcgctg tacacgctgc tgatgctggt tcctgcctgt atgctgctgg
     9721 tcaacggacg caggcagaaa cgccgtccga aagctgtggc attcgcattg ggcggcatat
     9781 tgctgatagg cggcgcgtgg ttcggctggc agggcgcaaa cggcaaaacg accgcgctgc
     9841 accatttcct gaccctcaat ccgccggccg aagcaggcaa atcttcggaa cacggaaaaa
     9901 tgtttgccga tactgccgcg ctgaaggcag cgatggatac ggcgttgaaa gaacatcccg
     9961 acaaacccgt cgttttggat ttttatgccg actggtgcat ttcctgcaaa gaaatggcgg
    10021 cttacacgct caatcagccg gaagtgcatc aggcagtcga tatggaacgc tttttccaaa
    10081 tcgacgtaac cgccaacaag cccgaacatc aggcgttgtt gaaagaatac ggtctgttcg
    10141 ggccgccggg cgtgtttgtc gtccgctccg acggcagccg cagcgagccg ctgctgggtt
    10201 ttgtcaaagc agacaagttt atcgagtggt atgaacaaaa ccgctgattc cgatgtttca
    10261 aatgccgtct gaagttctca gacggcattt ttgtcagaaa ggaaaacggc aatatccact
    10321 atacacacgg aagcagtcta aagatgtgaa aaccgtgtgt ataagttagt ttgcgtattg
    10381 aaattaaaag ataaaaatac gatgattaaa aaataggcat aagaaaagat aaacacagcc
    10441 ggtgtttgcc ggtgtgtgta tcttttgtgg aaaaacatct taatgtcatg tattttatgg
    10501 aaaataataa catgattaaa aaatagtcaa tcattatagt ggattaaatt taaatcagga
    10561 caaggcgacg aagccgcaga cagtacagat agtacggcaa ggcgaggcaa cgccgtactg
    10621 atttaaattt aatccactat aaggttcagg taagcggctg caaccgtcaa tgtctgaaaa
    10681 gaggcagtgc tgtttaagca tggaaagccg gataaaaaaa actgcctccg tttccggagg
    10741 cagttcgtgt gcggataaaa ccttacagac cgctcaaacg ggcagtgtcg tgggcaatca
    10801 tcagctcttc gttggtcgga atgaccacgg caacggcttt gctgtcggca gtggtaatca
    10861 cgccggcgtt gccgaagcgg gctttcaggt tggcttcttg gtcgatgttc agaccgagga
    10921 agcccaagta gccgatcacg cgttcgcgga tgatgtcgga gttttcgccg atgccgccgg
    10981 taaagaccag tgcgtccaaa ccgcctgcgg caaccgccat actgccgatg tatttggcaa
    11041 ggcggtagat aaacatatcc aaggccaatt tcgcgccttt atgccccttg gcggcttctt
    11101 cttcaatggt gcggcagtcg ttggacaggc cggaaatgcc gagcagaccg gattttttgt
    11161 tcagcatttc agtgatttgg gcgatggtca tattggcgtt ttcggcgaga aagccgaata
    11221 cggaaggatc gatgtcgccg ctgcgcgtac ccattaccag cccttccagc ggggtcaggc
    11281 ccatactggt gtcgcgcgat tcgccgttgg cgacggcggt aatggacgcg ccgttgccca
    11341 agtgggcaat gaccatacgc aggtcttttt tgtctttgcc gaggaagcgc gcggtttcgt
    11401 cggcgacgaa gcggtagctg gtaccgtgcg cgccgtaacg gcgcaggccg tatttttcat
    11461 acaactcctg cggaacggcg tatttgtagg cgacttcggg catggtttgg tggaaggagg
    11521 tatcgaatac gacgacgttg ggcaggtctt tgaaaatgct ttgcgcggca cgcaggccca
    11581 agaggtgggc ggggttgtgc aggggggcga gcgggatgca tttttcgatg ccggcaatga
    11641 cttcgtcgtc aacgaggatg gattcgctgt acagttcgcc gccgctgacg acgcggtggc
    11701 cgatggcgcc gatgcggctg tcgaggccgt gggctttgag ttcttccatc agggcttcga
    11761 ccgcgccggt gtggttggga tgtgcggaca gatcgacttt gtgtttttcg ccgtttactt
    11821 tgaatgtgat gtaggcatcg ggcaggttga gtttttcggc aaggcagctg agcaggactt
    11881 cgccgctgcc gttatccagg accgcgcctt tgagggacga gctgccgcag ttcaaaacca
    11941 agatcaattt ttgggacatt ttcttactcc ggaaagtttc agacggcatt ggaatcggac
    12001 acggatacta accggattcg tgccgaatcc gttttgcctt cctgggcggg aaagtagtgg
    12061 ggccgtctga aaaggttgat aaaagaagca ggctattcta gcaaaaaatc tttgcaattg
    12121 cttggcttaa tcgggcgttt gcgtgaaaat ggcggaagtc gggcattttg acggaaaaaa
    12181 gggggatatg cggtttgccg gtgttttcgg ctgggggagg gagaatttct ttgcattctt
    12241 cccgtccaaa ctggtacatt cgccctgttt ttagttggtg tcgtctgaaa aaaggcgggt
    12301 gggaaattta tgcattattt cagtattcat gaccaatcgg gacggcatat aggctttttc
    12361 atcctgcttg ccgacgacga atcggaagca cggccgcaaa gcgggcgttt tgccgtcaaa
    12421 cttgaaggcg gaatggaaaa tcatgtcctg tcgccgttcg gtcagacgga aatcccccaa
    12481 tattggcgcg tggtcaaaga ccgtatcgag ctgttttttg acgaagctcc cgtcggaact
    12541 ttgcgtaacg aatatctgac cgtgtcgggg cagacttttg ttttgaacga tttgacaggg
    12601 aatatgtgat ggaaagcatg tttatattgg tgcccatcag cattattttg gcgtttgtca
    12661 tcggctggtt tttttggtgg tcgggcaaaa acgggcagtt tgacgacttg gaagggccgg
    12721 cgcaccgcat cctgatggat gatgattcga caagtaaact gattgaaaaa gagagggaaa
    12781 acgatgtacg ctaaaaaagg cggtttggga ctggttaaaa gccgccgttt cgcacctctt
    12841 ttcgctacgc agtttctcgg cgcgttcaac gacaatgtgt tcaaaaccgc gctgtttgtg
    12901 atgattgggt tttacggttt ggggcaaaac ggcttcctgc ctgccggaca gatgttgaac
    12961 ttgggcgcgt tgttgtttat tttgccgtat ttcctgtttt cctcgctgtc ggggcagttg
    13021 ggtaacaaat tcgacaaggc cgttttggcg cgttgggtca aggtgctgga aatgatcatt
    13081 atggcggtgg cggcatacgg gttttatatc cggtctgccc cgctgctttt ggcgtgtctg
    13141 ttttgcatgg gcgcgcaatc gacgctgttc gggccgctga aatacgccat cctgcccgat
    13201 tatctcgacg acaaagagtt gatgatgggc aacagcctga ttgaatcggg tacgtttgtc
    13261 gccatcctgt tcggtcagat tttgggaacg gcggtggcag gtgtaccgcc ttatattgtc
    13321 gggatactgg ttttgctggt cgccgtcgga ggcacggtcg gcagcctgtt tatgccgtcc
    13381 gtccccgcca aggctgccga tacccaaatt gagtggaata ttgtccgtgg cacaaaatcc
    13441 ctgcttcgtg aaacggtgcg gtacaaaccc gtctttaccg ctattatcgg tatttcgtgg
    13501 ttttggtttg tcggcgcggt ttataccacg caactgccga cctttaccca aatccatctg
    13561 ggcggcaacg acaatgtttt caacctgatg cttgccctgt tttccatcgg tattgccgcc
    13621 ggttcggtac tgtgtgccaa gttcagcagg gaacgcctga tgttggcttg ggtaacggtt
    13681 ggtgcgttgg gtttgacggt ttgcggcctg gttttggtgt ggctgacgca cggacaccgt
    13741 tttgaagggc tgaacggcat tttttggttt ttatcgcaag gatgggcata tcccgtgatg
    13801 gcggtgatga cgctgatcgg ctttttcggc ggatttttct ccgttccgct ctatacctgg
    13861 ctgcaaaccg ccagtagcga gactttccgc gcccgcgccg ttgccgccaa caatatcgtt
    13921 aacggtattt ttatggtttc cgccgccgtt ttgagcgcgg tgttgctgtt tttgtttgac
    13981 agcatttcct tgttgtatct gattgtcgct ttgggcaata ttccgttgtc ggtatttttg
    14041 attaagcgcg aaaggcggtt tttaggcgcg gcggcaatca ggaaaaaacc ttgaatgccg
    14101 tctgaaaaaa ttgtccccaa aatacacaat ccgcctgccc aatgttgtga acacactgtg
    14161 aataagtggg tttgtgcatt gaaatgaaaa cagaaaaata tattgcttaa aaaatagtca
    14221 gcagatggaa aatttgaaga aagaagcaga catgattcaa cgccaaatca tcctcgatac
    14281 cgaaaccacc ggtctgtatg ccgacggcgg cgaccgcttg gtcgagtttg ccggtttaga
    14341 aatggtcaac cgccaaatga ccgacaaaaa cctgcacctc tatgtccacc ccgagcgcga
    14401 tatgcctgaa gaggcggcga gggtgcacgg tctgacgata gaggttttgg aagggaaaaa
    14461 cgcaccgcct tttgcagagg tcggcaggca gattgccgat ttcctgcgcg gtgcggaact
    14521 gattatccac aatgccaaat tcgacgtggg cttcctcaat atggagttcc gccggatggg
    14581 gttgccgacc gttgaggaac tcggctgtac cgttaccgat accctggcga tggcgcgcga
    14641 aatgttcccc gggcagaaag ccagcctgga tgccctgtgc aaccgttttt ccgtcgaccg
    14701 cagcaagcgc gtcctgcacg gcgcattgat cgactgcgaa cttttgggcg aagtctatct
    14761 tgctatgacg cggcggcagt tcgacctgat gggtgcggcc gcagaggaaa aaatggaaac
    14821 caaacccgtg gtgcataccg aaaccaaacg cagcggcaaa ctgaaagtca tcagggcgga
    14881 cgaaaacgag ttggcggcgc acgggcaata tttggacggc ttgggcgaag cctgtatctg
    14941 gcgcaaggaa gccgtgccgt ctgaaaacgg cgggacggac gcatgaagcg caaaaatatc
    15001 gcgctgattc ccgccgccgg catcggggcg cgtttcggtg cggacaaacc caagcaatat
    15061 gtcgaaatcg gaagcaaaac cgttttagaa catacgcttg ggatttttga acggcatgag
    15121 ggcgtcgatt tgaccgtcgt tgtcgtctcg cccgaagaca cgtttgccga taaggttcag
    15181 acggcatttc cacaggttcg ggtgtggaaa aacggcggac agacccgcgc cgaaaccgtc
    15241 cgaaacggtg tggcaaaatt gttggaaacc ggtttggcgg cggaaaccga caatattctg
    15301 gtacacgatg ccgcgcgttg ctgcctgccg tctgaagctt tgacgcggtt gatagaacag
    15361 gcgggcaacg ctgccgaagg tgggattttg gcaattcccg ttgccgatac gctcaagcgc
    15421 gcggacggtg ggaacattag tgcaaccgtc gagcggacga gcctttggca ggcacaaacg
    15481 ccgcagcttt tccgcgccgg gctgctgcac cgcgcattgg ctgcggaaaa cttggacggc
    15541 attaccgatg aagcgtccgc cgtcgaaaaa ttgggcatcc accctttgct ggtgcagggc
    15601 gacgcgcgca atttgaaact gacgcagccg caggacgcat acatcgtcag gctgctgctc
    15661 gatgccgtct gaacttcaat tcaaaacatc aggaacacaa acgatgacga acatccgtat
    15721 cggacagggc tacgatgtcc accaactgac cgaaggcaga aagctgatac ttggcggagt
    15781 tgaaatccca tttgaaaaag ggctgctcgg acactccgat gccgatgcgc tgctgcacgc
    15841 cgttaccgac gcgctgctcg gtgcggcagg tttgggcgat atcggcagcc atttccccga
    15901 caccgccgca gagttcaaag atgcggacag ccgcgtcctt ttgcgtgcgg cgtatcaaag
    15961 cgttcaggcg cagggttggc aggcggtcaa tgtcgatacg accgtgattg cacagaaacc
    16021 caaactcgcg ccgcatattc cgcaaatgcg tgccaacatc gccgccgatt tgggcataga
    16081 catttcgtgc gtcaacatca aaggcaaaac caacgaaaaa ctcggttatc tcgggcgcat
    16141 ggaaggcata gaggcgcagg cggcggtatt gctggtacgg atttgaacgc ggcgggcagg
    16201 acgtgcaaaa aaatgttaag atggcggctg tttgttgata cggtttccca aaggacaaaa
    16261 atatgacgac acaagacgaa ctcaagcgca tcgccgccga aaaagcagtc gaattcgtac
    16321 ccgaaaacga atacatcggc atcggtaccg gctcgaccat caactttttc atcgaagcct
    16381 tgggtaagag cggcaaaaaa atcaaaggcg cggtatctac ttccaaaaaa tccagtgaac
    16441 tgcttgccca atatgatatt cccgtcgttt ccctgaacga agtatcgggg ttggcggtct
    16501 atattgacgg tgcggatgaa gtgaaccacg ccctgcaaat gattaaaggc ggcggcggcg
    16561 cacacctcaa cgaaaaaatc gtcgccagcg catcagaaaa attcgtctgc attgcggacg
    16621 aaagcaaata tgtttcacgt ttgggcaaat tccctctgcc cgtggaagtc gtcgaaagcg
    16681 cgcgttccct tgtttcgcgc aaactgctcg ctatgggcgg acagcctgaa ttgcgtatcg
    16741 gctacaccac tttttacggc aaccaaatcg tcgatgtcca cggcttgaat atcgacaaac
    16801 cgctgatgat ggaagacgaa atcaacaaaa tcaccggcgt actcgaaaac ggaatattcg
    16861 cccgcgatgc cgccgatgtg ttgattttgg ggacggaaga gggtgcgaaa gtcatctatc
    16921 cctgccaagg gtaatcctga cgttaagatg ccgtctgaaa tccggttcag agatgttcag
    16981 acggcatcgg catttgaaaa atatgcagat taaaaaaatc atgaaatggc ttcccgtcgc
    17041 cctgtcgctt ttgggtgcgt tgggttatac ggggtacggc agcgaggcgg tgcggacggc
    17101 ggttgccgta ctcgacgtac tcggcgcggc aggggacgcg ggttccgacg cgcccgcccg
    17161 ccgccgagca tcggcgaaat ccggccaccg ctacacaggc acggtgtcca aagtctatga
    17221 cggcgacacc cttcacgtta tcgacggcga cggcgcgaaa cacaaaatcc ggatggcgta
    17281 tatcgacgcg ccggagatga aacaggctta cggcacgcgt tcgcgcgaca acctgcgcgc
    17341 ggcggcggaa ggcaggaaag tcagcgtgcg cgtgttcgac accgaccgct accagcgcga
    17401 agtggcgcag gtttctgtcg gcaaaaccga tttgaacctg atgcaggtgc aggacggggc
    17461 ggcgtggcat tataaaagtt atgctaaaga acagcaggat aaggcggatt ttgccgatta
    17521 tgccgacgct caaattcagg cggaaaggga acgcaaagga ttgtggaaag ctaaaaatcc
    17581 gcaagcgccg tgggcgtacc gccgggcagg caggagcggc gggggcaata aggattggat
    17641 ggatgccgtg ggcgaatggt tgggcatttg gtaaagacag cgcacggctt tgaacggcaa
    17701 atgccgtctg aaaaccgcgc gccctgcatt tttgcggaga cgcgcaaccg ccgtctgccg
    17761 cgataaaaaa gttcaggcgg cggtttttac ggtaaaatgg cggattattt tgcaacggcg
    17821 gcagttccgc cggtttttga tacggacggt atatggattt tcgttttgac attatttacg
    17881 aataccgctg gatgttcctt tacggcgcac tgacgacctt ggggctgacg gtcgtggcga
    17941 cggcgggcgg ttcggtattg ggtctgttgt tggcgttggc gcgcctgatt cacttggaaa
    18001 aagccggtgc gccgatgcgc gtgctggcgt gggcgttgcg taaagtttcg ctgctgtatg
    18061 ttacgctgtt ccggggtacg ccgctgtttg tgcagattgt gatttgggcg tatgtgtggt
    18121 ttccgttttt cgtccatcct tcagacggca ttttggtcag cggcgaggcg gcaatcgcgc
    18181 tgcgtcgcgg atacgggccg ctgattgccg gttctttggc actgatcgcc aactcggggg
    18241 cgtatatctg tgagattttc cgcgcgggca tccagtctat agacaaagga cagatggagg
    18301 cggcgcgttc tttggggctg acctatccgc aggcgatgcg ctatgtgatt ctgccgcagg
    18361 cattgcgccg catgctgccg cctttggcga gcgagttcat cacgctcttg aaagacagct
    18421 cgctgctgtc ggtcattgct gtggcggagt tggcgtatgt tcagaatacg attacgggcc
    18481 ggtattcggt ttatgaagaa ccgctttaca ccgtcgccct gatttatctg ttgatgacga
    18541 ctttcttagg ctggatattc ctgcgtttgg aaaaacgtta caatccgcaa caccgctgat
    18601 tggagtcata tcaacaaaaa gctgcccggg tattcatttg aggcagcttt tttgtggcaa
    18661 atttat
//
LOCUS       DAWFAD010000029        18406 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWFAD010000029 DAWFAD010000000
VERSION     DAWFAD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18406
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.029"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(287..4456)
                     /locus_tag="WGO49_000730"
     CDS             complement(287..4456)
                     /locus_tag="WGO49_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ0305865.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTLWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNINRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGTLKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGRRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
     gene            complement(4511..6358)
                     /locus_tag="WGO49_000731"
     CDS             complement(4511..6358)
                     /locus_tag="WGO49_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ0305866.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLHSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6770..7999
                     /gene="sstT"
                     /locus_tag="WGO49_000732"
     CDS             6770..7999
                     /gene="sstT"
                     /locus_tag="WGO49_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ0305867.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAYTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8111..9604
                     /locus_tag="WGO49_000733"
     CDS             8111..9604
                     /locus_tag="WGO49_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0305868.1"
                     /translation="MFKRSVIAMACIVALSACGGGGGGSPDVKSADTLSKPAAPVVTE
                     DVGEEVLPKEKKDEEAVSGAPQADTQDATAGKGGQDMAAVSAENTGNGGAATTDNPEN
                     KDEGPQNDMPQNAADTDSSTPNHTPAPNMPTRDMGNQAPDAGESAQPANQPDMANAAD
                     GMQGDDPSAGENAGNTADQAANQAENNQVGGSQNPASSTNPNATNGGSDFGRINVANG
                     IKLDSGSENVTLTHCKDKVCDRDFLDEEAPPKSEFEKLSDEEKINKYKKDEQRENFVG
                     LVADRVEKNGTNKYVIIYKDKSASSSSARFRRSARSRRSLPAEMPLIPVNQADTLIVD
                     GEAVSLTGHSGNIFAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGE
                     VLHFHMENGRPSPSGGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAVIDGNGFKGT
                     WTENGGGDVSGRFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(9680..9895)
                     /locus_tag="WGO49_000734"
     CDS             complement(9680..9895)
                     /locus_tag="WGO49_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305869.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(9888..10103)
                     /locus_tag="WGO49_000735"
     CDS             complement(9888..10103)
                     /locus_tag="WGO49_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEZ0305870.1"
                     /translation="MKEYKVVIYQESQLSSLFFGAAKVNPVNFSAFLNKQTPEGWRVV
                     TMEKDLRRMLLFFKREAYVVILERDRV"
     gene            complement(10178..11521)
                     /gene="argG"
                     /locus_tag="WGO49_000736"
     CDS             complement(10178..11521)
                     /gene="argG"
                     /locus_tag="WGO49_000736"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ0305871.1"
                     /translation="MSQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEVSPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKK"
     gene            11646..12446
                     /locus_tag="WGO49_000737"
     CDS             11646..12446
                     /locus_tag="WGO49_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ0305872.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALELPVVRHPEAAGFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLDTYYAGRFNQTQRGSRSVYVFEQPESRITPIMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            12486..13472
                     /locus_tag="WGO49_000738"
     CDS             12486..13472
                     /locus_tag="WGO49_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ0305873.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDMMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFQKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPDIPVYLVAEDMCASG
                     CYYIAAAADKIYADSSSIVGSIGVIGSSFDATELMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKAGVREALQAVR"
     gene            14073..14363
                     /locus_tag="WGO49_000739"
                     /pseudo
     CDS             14073..14363
                     /locus_tag="WGO49_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981114.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14430..14804)
                     /locus_tag="WGO49_000740"
                     /pseudo
     CDS             complement(14430..14804)
                     /locus_tag="WGO49_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14977..15333)
                     /locus_tag="WGO49_000741"
     CDS             complement(14977..15333)
                     /locus_tag="WGO49_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ0305874.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNEKTVIFHF"
     gene            complement(15395..15511)
                     /locus_tag="WGO49_000742"
                     /pseudo
     CDS             complement(15395..15511)
                     /locus_tag="WGO49_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(15598..15810)
                     /locus_tag="WGO49_000743"
     CDS             complement(15598..15810)
                     /locus_tag="WGO49_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305875.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(15886..16245)
                     /locus_tag="WGO49_000744"
     CDS             complement(15886..16245)
                     /locus_tag="WGO49_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0305876.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(16242..16460)
                     /locus_tag="WGO49_000745"
     CDS             complement(16242..16460)
                     /locus_tag="WGO49_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305877.1"
                     /translation="MMTESHGYLSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYCWLGFDELPQSEKIKFLSYLNIL"
     gene            complement(16481..16687)
                     /locus_tag="WGO49_000746"
     CDS             complement(16481..16687)
                     /locus_tag="WGO49_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305878.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKDLPIR"
     gene            complement(16653..16853)
                     /locus_tag="WGO49_000747"
     CDS             complement(16653..16853)
                     /locus_tag="WGO49_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305879.1"
                     /translation="MASDLLSKQTSDKILGYQTEHRRVRYDINNNIYVLANPKTFKIK
                     TMFKPNLGKKYYDGEFKKDMGN"
     gene            complement(17213..17557)
                     /locus_tag="WGO49_000748"
     CDS             complement(17213..17557)
                     /locus_tag="WGO49_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305880.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(17595..17918)
                     /locus_tag="WGO49_000749"
     CDS             complement(17595..17918)
                     /locus_tag="WGO49_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305881.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYEAFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(17921..18151)
                     /locus_tag="WGO49_000750"
     CDS             complement(17921..18151)
                     /locus_tag="WGO49_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ0305882.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENTRTGEKTDFKFIENTRTGEKTDFKFKD"
     gene            complement(18230..18406)
                     /locus_tag="WGO49_000751"
                     /pseudo
     CDS             complement(18230..18406)
                     /locus_tag="WGO49_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT         4270 a         4374 c         4888 g         4874 t
ORIGIN      
        1 acctgtgtat cgctgtaaat cttagagatg gcggaatata gcggattaac aaaaaccggt
       61 acggcgttgc ctcgccttgg ctcaaagaga acgattctct aaggtgctca agcaccgagt
      121 gaatcggttc cgtactattt gtactgtctg cggcttcgtc gccttatcct gatttttgtt
      181 aattcactat atcgattctg ttcccttgca ggcggacggc ggcggcggac ggtggaaaag
      241 caggttttca aatgtccggt ttggcggcgc gccgtctgaa aaccgcttat tttcctttgc
      301 cgtttccggc ggagtctttt ttgtccgaac cggagaagcg gtcgaaacgt atggtgtatg
      361 tcagctcgcc gcccgacgaa cgtctgccga tacgggcaac cgcctgtatg gcgcgggtca
      421 gccggtaaat cagtttgacg gactgttccg cgctggagat gctgtattcg tagccgatgt
      481 agagtttgcc ggtcagttgt ttgccgacgg tcagcacctg ttcggcgggg ttgagttcgc
      541 cggtttgcgc gttgcggctg cgcttgctgg taaagcccaa atcatccacc agcccgatgc
      601 ggtcgttgat ttgcccggca agcagcgcgc ctgcggctgc ggacagggcg gcattgtcgc
      661 cgctgctgcc actgccggcg cggttgagga tgagccagga gagcttgtct ttttcactca
      721 tcggttcgtt tgccgtcagc gtaatgcgcg gactgttgag gctgcccaat atttccacgc
      781 ccgcaccgac gggggaaagg cggcgttcgg cgcggatgtt gaggttgggg tcgttgagcg
      841 ggccgacaaa ggagaccgtg cctttggtaa tgtccaaatc ctgcccgtat gccttataac
      901 gccctttgat gacgcggacc gtgcccacgc cccgcacgct tccgcccgat tgggcggtca
      961 gggtcagttt gccgcctatg gtaacgtccg cgccgtagcc ggcgaagcgg atgccgtcat
     1021 tgaggtctaa agtcaggttc atattgacgg ggagcggtgc cgccgcctct tttttaactt
     1081 cgcctaatac gacgacatcg tcgccgacgg acggcatcga ggatttttgc gaaccgaaca
     1141 gcccctgatc cgttttaatc atcccggtaa cggatatgcc tttttgcggc gaatagcgca
     1201 ggcgggtgtt gccggaaacc gtcaggcggc ggttagggcg ggacaggatg cggtatttgt
     1261 cgaacaccgc gccgatatcg acatcgggtc cgctgttttc cataccgacc gtgccggaga
     1321 gttccgccgt tccttcgtgc cggaatttca ggctgtcgat tacccatttt ctgcccgcga
     1381 tatgcgaacg cagcgagccg ttgtccaaga tgatgccttg ggtttggttg cggtaataga
     1441 gcttgtcgcc gttgatgctg ccgcccaagt gcgggtcggc gatgctgccg ccgagggtta
     1501 cggcggcatt caggctgcct ttgacggttt gtccgacggg caggaagttg cggaatactt
     1561 cggcatcggc aacggtcagg ttgagcctgc cgcccaaagg cgcggtatcg aaagagcggc
     1621 tttgcccgac ggtgatgttg ccgttgattt tcccgtagtt gctgctgccg ttgacggcgg
     1681 cattgacgga cggagagccg acccgtccgc cgatttgcgc ggcggcattc aggctgccgg
     1741 taatgttttg cgcggcggcg ggcagaaagg gcttcaatgt gcccaagtcg ggaagggagg
     1801 cggtaatcct gccaccgagc ggtgcatttg ccatattgcc gccgaaggcg ttgccgatgt
     1861 ccaaatcggc gttaatccgc ccgaaacgtg cgccgccgtc aagcaggatt ccgatacggt
     1921 cgttttgaaa gcgcgttttc agggaaaatg cgttcaaacc caaagcctgc ccgccgggca
     1981 atacggcatc gccgctttgc cgattgatat tgaggtagcc gcgcgcgttg cgcccgtagg
     2041 cgacatccca gtcgccgttt aaaaccagat tgtgttcgaa gggcggtttg aagaaattgt
     2101 gcaactcggc gatatgcaga cctcgtgcgc cgccttttgc cgatatgccg gttttcctgt
     2161 cccaggaaaa gtgttgcagg ttgaggctgc cgcccatagc ctgccaattt gccgcacttg
     2221 ccgccacacg ttccgcaccg gcttcgagcg tcatacggtt ttgcagtttt aggttgaatg
     2281 cgccgccgat gtcgaggatg ccgatgctgc ccttccatag ggtaagttcc ctgttgatgc
     2341 cgcctgaagc gtccaaatcg aatttgaacg gtttgccatc cagcgtcatg gcggcgtgtg
     2401 tgcggatgcg gtgctgcacg cccgtgccgt ccagcatcag gtcggcggta tcgacaaccg
     2461 ccgctccgcc cgacagcgaa aggcggctgc ctttgatgtc ggcgcgtatc gggcggcttg
     2521 tgtcgggcga acctttgagc gtgaaatcga gcgaacggat gtctgccgcc ttgccgatgt
     2581 gcaggttgcg cgccgcgccg gaaaggtcgg tttcaaaggt tcggatgccg ccgtccaaat
     2641 cgccggaaag gtgtccgcgt acatttaaag accccgcgag tccgaaaccg aaacgggata
     2701 aatcgggtgc ggtgatgttg aggttgagcc ggtcgccttt tttgccgaag ccgccgtctg
     2761 ttttaataat gttccgcccc agccgcaaat cgacggcggc acgcggaagg tggcgggact
     2821 cgtaaacaat gtcggcactg ccggcaatcg gtacgccgtt gaacgtgccg ggtaaaaacc
     2881 gcattttgcc tgtgaatttc tcttttgcca gttcgccggc aaggtttatt gagccgttga
     2941 tattgcctgc cggaagttgc ggattgatgc gcgaagggtc gaatgcgcgg gaacggatgt
     3001 ccagcttgag caggcggtct ttaaacagct cgagatagcc ttgcgcggtc aggctgcctt
     3061 gcccggcggc gatgttgacg gtgtcgagca ccagtttccg ctgtccgttt gcggggtcgc
     3121 ttgcaatggc gaggctgccg tccgtgcgcg ccgtgccgat gccgagttgc caagagattt
     3181 tgggcgaggc ggtcgtgcca ccgatgccga tgctgccgtc caacctgcct ttgaacgcgg
     3241 tttgcagtac gtcttccgcg ccgacggagt tgatgcctat atttaaatcg aggatgtctt
     3301 tttcggtgtc gattttgccc gacagcctga tgccgccccg tccgagcagg gcggcggacg
     3361 tattgccgat atgcaccgtg ccgtcctgcc ggatgacaaa gccgcctaaa acctgacgga
     3421 cggggatgcc gttgcggtcg gcaaagccgg ctttggtgtt ttccaaatcg agcgaacctt
     3481 ccagcgcgat gccgtctgaa aacgacggga tggcggtcag gtcgaaattc agcccggcat
     3541 cgggcaggga gggcacgaag gcggccgggt tgatgttgaa ccctttgacc agcacttctt
     3601 ccaatgtttt atccaatgat tcggcaaacg ggtggatgac ggattttccc gagaggcgga
     3661 tattgccgcc gtcgatcgcc agttcggcgc gcacatcctt caggctgccg ctcagccgag
     3721 ccgtactgtg tatggttttg ccttcgagtc cgcctttggt gtaaatggcg gtatcgaggg
     3781 caaacggttt tttcaagccg accgaggctg accccgacga actgctccac ggcgtgtcgg
     3841 cagccttcag gtcgaggcgg tgtcctttgc ggtcgtaacg gtatgaagca tccagccgtt
     3901 cgagatagac ggtttgtttg tcaaaggctt tgcccatgct gattttgccc gtctcgaagc
     3961 ggtcgagata gacggcggca ggcaggtcta tgctgtcggg aaggctgagc ggcgggcgtt
     4021 cttctttagg cggagtcggt ttggtaacga tggcgatgtc gccggcggaa atttcggtaa
     4081 tgtgcaggct gcggcgcatc agttcggacg gtttccacgc gaagcggaag cggctgattt
     4141 taaggtctgc cccctcggtt tctatcgacc agttgtcgcc gtcgaagccg tcgagcagcg
     4201 tgcctttgag gttttgggag gaaatgttta cgccgaacca agacgggatt tggtacagcc
     4261 cgaagcgcaa acccgcttcc gtgccggcga gccagccgag gaaacatact gccaaaatca
     4321 gaacagacag cagtgccgcc gacagcttca gcagcgggcg gcgttttttt gccggcgggg
     4381 tagggcggtg ttcagacggc attttgcgcg tgccgttttc ggtcggatcg gtatctgtcg
     4441 gtgcggtatc ggtcataatg tctgtttcaa aatggtttgc tgcaatgccg tctgaagcgg
     4501 ccgtatcggt ttagaagcgc gttcccaagc tgatgtgcca gcggattttc ttgtcgctgt
     4561 gcccgtaggc gatgtcgaag gaaaacggcg cgagcgggct gaaccagcgc acgcccagtc
     4621 ccgaaccgtg tttcagcttc atacgtttga aattggcggc ggcgtcgccc atatcgtgga
     4681 acaccgcgcc ggaaagggtg cgcgtaaacg gcagttggta ttccaggctg cccaccaaga
     4741 gggcgcgttc gggcaggacc gatccgttcg ggccggcaag cccgatgctg tcgagttcgt
     4801 aaccgcgcac ggaagacgcg ccgccgctgc ggaacatcag ccctgaagga acgttggcat
     4861 tgtcgcgggc aacggtgtaa cccgcttgtc cgcgtatgat gaacgtgccg agttttttgt
     4921 tttcgggcgt gaagaaataa cctgcgcggg cagaggtgcg gatcagcgcg gtggaggaca
     4981 ggaatgcgcc caaagtcgta ccgattttgc cgtcgaggta atggccgttt tcgggatgca
     5041 gcacgttgtt gagcagctgg cgtttccaag aggcggtcag catcgtggcg tggctgttgc
     5101 ccaaatcgat atccgagccg gggattttcc ggccttctgc gagaaactcc gcccccagcc
     5161 tggcatcgat gcccgcgcgg tcgcgcacat accagatgcc gccggagaag gcgcgttttt
     5221 cgaggttttg ggtggtcgaa cggttgtagg aaacgttgct tgtccagtag ttgccccgat
     5281 agttgcgcgg ctggctgatg ccggcggcaa gcgtggtttc gtatttgtcc atatcccaga
     5341 cgaccgaacc gatatagcct ttgttgaaga ggttgtaata gtcgtaggcg attttgccgc
     5401 ccaaaccgta ttccgaatcg aggcggatgc cggtttcgag cttgtggcgt ttgacctcgg
     5461 ttacgctgac tttgacgggg acgcggtcgc cttggaggcg gtcgaagtcg gcttgtacgg
     5521 acgcgccgga ataatgcccg ttttgttcga gcgcctgttg gaagtcgagc agcaggtcga
     5581 ggtcgtaggg cgtgcccggt tggaagcgcg ccaagccgga gacgatttgt tcggggtaac
     5641 gctgcgtgcc ggtaatttca aagtcgccga aggcgatggg gcggccgctg tccacgacga
     5701 cgttcaaatc ggcggtggcg gtatcggggt tgacggccgc ccgggtgttg ccgagcttgg
     5761 caagcgggta ggctttgcgc gttaccgcgc cgaggacgga agttttgctg ttttcccaac
     5821 tgtcctgatc gaaatcactg cctaccggct gctgccagtt ttccagcgcg ttgcggtagt
     5881 attcggcgag gttgccgtct gaaaggatgt cgccgaggat ggcgacgccg acgttggcga
     5941 ttttggtgcg cgggcccggt gtgatgtgta ccgtataagc tccgtctttt tccgtcaggc
     6001 tgactttgct gctgaaatag cctttgctgt ggagcattgt tttaacgttg tccggtgctt
     6061 cttcggcgag gaagcccgtc tgttccttgt ccaatacttc ttcctgctgc tgcgtgatga
     6121 gcggcaggtg ttcttcgacc atatctttga tttcactatc ctgcgtgtcg atgcggacgg
     6181 ggaatttggg ttttaattta actgattcgg tgtcggggct tttgtttttg aacaatgcga
     6241 aacccgccgc cttgttttcg gaaaggtcgg cggcaggcgc gtatgcgtgc ggaaagaaaa
     6301 ataaagccgg caggagcagg gcggtcggtt taatcatcat ggtacgggtg tcgtgcatcg
     6361 gtgtggcggt tttcgattct aacactgttc ggacagggtt tcgaacggcc gtttttgtgt
     6421 tgttcgtacg gggcgggacg cttcagacgg catactgccg ccggtatttc ggaggttgcg
     6481 gcccgccgga aatgtatctg cttgttttaa ggtattttgt ttctaatccg catatggaaa
     6541 acaggctgcg tttcggaata gtgccggaaa ataggcagga ttaaataaat ttattttcgt
     6601 tcaataaatt aaaccgttgc gccgaatgat aaagcaaagc ctgtccggca ctgcataggg
     6661 caaatcaacg caaccttaaa acacagccga aaaataaaaa aaaacacaga gaatgagttt
     6721 tcttttaaaa tacaacattt tttaacatat cacaacataa ggaagaatta tggcgttcgg
     6781 caaatcctta tttcatgcga tcggccgcgt cagcctggtc aggcagattg ccgccggttt
     6841 ggcgttgggc atcgtaatcg gttcggtttc cccgcaactg ggcttggcgg caggcttgtt
     6901 cggcagcctg tttgtcggcg cgctcaaagc ggtcgcgccg gttttggtat ttattttggt
     6961 ggcggccaca atcgcgcagc accaaaaagg caacaaggcg catatcaggc cgattatcgt
     7021 cctttacctc atcggtacgt tttccgcagc cctgaccgcc gtcatcgccg gtatggtttt
     7081 cccgacgcac attgttttgg cgggcgcggg cgatgtgtcc gccgcgccgc cttccggcat
     7141 tgtggaagta ttgaaatcgc tgctgatgaa cctggtcgcc aacccgatta acgcgattgc
     7201 caatgccaac tatatcggca ttttggcttg ggctttggtt ttgggcgcgg cgttgcggaa
     7261 tcacggttcg gacgttacgc ggcaggtcgt tgccgatttg gcggaagcgg tttccaccgt
     7321 cgtgaaatgg attatccgtt ttgccccttt gggcattttc gggctggtgt cgtccacaat
     7381 cgcggagacg ggtttcggcg cgctggcggg ttatgcgaag ctgcttgcgg tactgttggg
     7441 ctgtatggcg tttatcgcgc tggtggtcaa tcccgccatc gtgtggtgga aaatccgccg
     7501 caacccttat ccgctggtgt ttacctgcct gcgcgaaagc ggcgtgtatg ccttctttac
     7561 ccgttcttcc gccgccaata tccccgtgaa tatggctttg gcgaaaaaac tggggctgca
     7621 cgaagacact tattcgattt ccatcccgtt gggggcgacc gtcaatatgg gcggcgcggc
     7681 gattacgatt accgttttgg ctatggcggc ggcgtacacc caaggcatac aggttgattt
     7741 tgccaccgcg ctgctgttga gcctggtggc gacggtcagc gcgtgcggcg cgtccggtgt
     7801 ggcgggcggt tcgctgctgc tgattccgct ggcgtgcagc ctgttcggca tcagcaacga
     7861 tgtcgctatg caggtggtcg ccgtcggctt tatcatcggc gtgattcagg attcggcgga
     7921 aacggcgttg aattcttcaa ccgatgtttt gtttaccgcc gccgccgatt tgggcagaca
     7981 gagaaaccgg gcggaataaa ccaagctatg ccgtctgaag cccttttggc gttcagacgg
     8041 catattttta caattttatg ggcatatggc ggaaacaata ccggctgcct tgaatatgaa
     8101 ggagatgatg atgtttaaac gcagtgtgat tgcaatggct tgtattgttg ccctttcagc
     8161 ctgtgggggc ggcggtggcg gatcgcccga tgttaagtcg gcggacacgc tgtcaaaacc
     8221 tgccgcccct gttgttactg aagatgtcgg ggaagaggtg ctgccgaaag aaaagaaaga
     8281 tgaggaggcg gtgagtggtg cgccgcaagc cgatacgcag gacgcaaccg ccggaaaagg
     8341 cggtcaagat atggcggcag tttcggcaga aaatacaggc aatggcggtg cggcaacaac
     8401 ggataatccc gaaaataaag acgagggacc gcaaaatgat atgccgcaaa atgccgccga
     8461 tacagatagt tcgacaccga atcacacccc tgcaccgaat atgccaacca gagatatggg
     8521 aaaccaagca ccggatgccg gggaatcggc acaaccggca aaccaaccgg atatggcaaa
     8581 tgcggcggac ggaatgcagg gggacgatcc gtcggcaggg gaaaatgccg gcaatacggc
     8641 agatcaagct gcaaatcaag ctgaaaacaa tcaagtcggc ggctctcaaa atcctgcctc
     8701 ttcaaccaat cctaacgcca cgaatggcgg cagcgatttt ggaaggataa atgtagctaa
     8761 tggcatcaag cttgacagcg gttcggaaaa tgtaacgttg acacattgta aagacaaagt
     8821 atgcgataga gatttcttag atgaagaagc accaccaaaa tcagaatttg aaaaattaag
     8881 tgatgaagaa aaaattaata aatataaaaa agacgagcaa cgagagaatt ttgtcggttt
     8941 ggttgctgac agggtagaaa agaatggaac taacaaatat gtcatcattt ataaagacaa
     9001 gtccgcttca tcttcatctg cgcgattcag gcgttctgca cggtcgaggc ggtcgcttcc
     9061 ggccgagatg ccgctgattc ccgtcaatca ggcggatacg ctgattgtcg atggggaagc
     9121 ggtcagcctg acggggcatt ccggcaatat cttcgcgccc gaagggaatt accggtatct
     9181 gacttacggg gcggaaaaat tgtccggcgg atcgtatgcc ctcagtgtgc aaggcgaacc
     9241 ggcaaaaggc gaaatgcttg cgggcacggc cgtgtacaac ggcgaagtgc tgcatttcca
     9301 tatggaaaac ggccgtccgt ccccgtccgg aggcaggttt gccgcaaaag tcgatttcgg
     9361 cagcaaatct gtggacggca ttatcgacag cggcgatgat ttgcatatgg gtacgcaaaa
     9421 attcaaagcc gttatcgatg gaaacggctt taaggggact tggacggaaa atggcggcgg
     9481 ggatgtttcc ggaaggtttt acggcccggc cggcgaagaa gtggcgggaa aatacagcta
     9541 tcgcccgaca gatgcggaaa agggcggatt cggcgtgttt gccggcaaaa aagagcagga
     9601 ttgatgctga tgccgcctga agcgggggcg ttcagacggc attgtgatgt ttgggttgcg
     9661 ggtccggttc aagtccgcat cagttgataa acccgtcgtc tttcattttc ttttcggaaa
     9721 aatactggcc gaataccagc aaggcggcga ggatgatgac aaacagcccc gccatagtca
     9781 gtgtgatttt gaagaacaat tccgcttcga gaaaagaaaa ccaaagttgc acaatcagca
     9841 gcagcaccca gccggcaaac agtccgagac aggcataaac gccaagctta aacacggtcc
     9901 cgctccaaaa tgacgacgta ggcctcgcgt ttgaaaaaca acagcatacg gcgcaaatct
     9961 ttttccatcg ttacaacccg ccagccttcg ggggtttgtt tgttgaggaa cgcgctgaaa
    10021 ttgacggggt tgacctttgc cgcgccgaaa aacaggctgg acaactggct ttcctgataa
    10081 atgacgactt tgtattcttt cataggattc cttttaacat aagggatcat cttttcagac
    10141 gaccccttaa gttgctaatg atgcaaaaca gcaaacctta tttcttattg cccaactgcg
    10201 gtaataccga gccttcgccc agcgacagca aaccgctttg cgagtagatg cccagtttgg
    10261 cgcgggtgtc ggtgatgtcg aggttgcgca tcgtgagctg tccgatgcgg tcgagcgggg
    10321 taaacgccgc accttcgact ttttccatac tcaggcgttc gggttggtag gtcaggttgg
    10381 gcgattcggt gttcagaatc gagtagtcgt tgccgcgccg cagttcgagg gtaacttcgc
    10441 cggtaacggc tttggcgacc cagcgttggg cggtttcgcg caacatcaag gcttggctgt
    10501 cgaaccagcg gccttggtag agcaaacggc cgaggcgcag gccgttgatg cggtattgtt
    10561 cgatggtgtc ttcgttgtgg atgccggtca ccaagcgttc gtaggcgatg tggaacaacg
    10621 ccatacccgg ggcttcgtag atgccgcgcg atttggcttc gatgatgcgg ttttcgattt
    10681 ggtcgctcat acccaagccg tggcggccgc cgatgcggtt ggcttcgagg aagagttcga
    10741 cggggtcggc gtattctttg ccgttcagtg caaccggcac gccttcttca aagcgcacgc
    10801 tgacttcttc ggggctgact tcaacgtttt cgtcccaaaa ggcaacgccc ataatgggtt
    10861 tgacgatttt gatgcccgag ttcaaaaatt ccaagtcttt ggcttcgtgg gtcgcgccga
    10921 gcatattgga atcggtggag taggcttttt caaccgacat tttgtagttg aagccgttgg
    10981 caatcagaaa ttcgctcatt tcgtgacggc cgccgagttc gtcgataaat tgctggtcga
    11041 gccagggttt gtagattttc agcgcgggat tggtgagcaa accgtagcgg tagaaacgct
    11101 cgatgtcgtt gcctttgtag gtgctgccgt cgccccagat gtttacgtcg tcttctttca
    11161 ttgcggaaac aagcatcgtg ccggttacgg cgcggcccag aggcgtggtg ttgaaatagg
    11221 cgatgccgcc ggtggaaacg tggaacgcgc cgcattggat ggcggcgatg ccttcgtgtg
    11281 ccaactgcgc gcggcagtcg attaagcggg cgttttccgc accgtattcc atcgcttttt
    11341 tgggaatggc gttgtagtcg tcttcatcgg gctgaccgag gttggcagtg taggcataag
    11401 gcagtgcgcc tttgagtttc atccacaaca gcgcggcgga ggtatcaagt ccgccggaga
    11461 aggcgatgcc gactttctga ccgacgggga ggttttgtaa aatagtatgg ttttggctca
    11521 tgaaaaaatc ctcggtagct tgaaaaatca aagccattat cttataaata ggaaatattg
    11581 tcagccatca tgccgtctga acgggtattc cgccgtttca ggcggtattt aaaaccgaaa
    11641 acattatgaa tgcgttcaac ctcatcatca tcggcgacga aatcttacac ggcagccgcc
    11701 aagacaagca tttcgccttt ttcaaatccc tgctcgaatc caaagggctg aagctcaatc
    11761 aggtgcaata tctgcccgac gaacccgatt tgctggtcag gcaactgcgc cgcagctttt
    11821 cagacggcat accgacgttt gttaccggcg gcatcggcgc cacgcccgac gatcataccc
    11881 gccaagcggc cgccgccgct ttggagttgc ccgtcgtccg ccatcctgaa gccgccgggt
    11941 ttatcgaagg cataacccag aaacgcggcg agccgcttga ttcgccggaa cacgcccaac
    12001 gcctgaaaat ggcagatttc cccgaaggcg cggaattggt gcccaatccg tttaataata
    12061 tcgccggatt ttccattcgc gagcattatt tcttccctgg ttttcctgtg atggcgcacc
    12121 cgatggcgga atgggttttg gatacttatt acgccggccg cttcaaccaa acccaacgcg
    12181 gcagccgcag cgtgtatgtg ttcgagcagc ccgaatcgcg cattacgccg attatggagc
    12241 atatcgagca aacttatccc ggcgtgcgtt cgtacagcct gcccagcgtc ggttggacgc
    12301 attcagacgg cacgcaggtc aaaccgcaca ttgagttcgg catcaaagcc gaaggagaag
    12361 ccgtcaacct tttggacgcg gcgtgggcgg aagtgttgca cagcttggac ggattgggag
    12421 cagagctgaa aaatcgggta aactgaaaac cgtttgccca aatcggacga tatttaagga
    12481 aaaccatgca ataccgaatc agaagagaaa acgaagcacc cgaagcaaaa aatgcggggg
    12541 aaaccttatg ggagcgcgat atgatgcgcg aagtgctgtt gtccgcctat caggacaggc
    12601 gcagggagcg gatgtggaaa aacatctggc gcgccgtcag caccctgatt ctggttgccc
    12661 tgattgcagg cattttccaa aaagacgaag ccgcattgca gttggcgggc aacacgccgc
    12721 ataccgcagt cgtcaacctg tacggcgaaa tcggcaacgg tgtagaagac caggtcaaaa
    12781 aactcaaaga cggtatggag gccgcctaca aaaacccgca ggcaaaagcc atcgtcatcc
    12841 gcgccaacag ccccggcggt tcgcccgtcg tctccaacac cgcttttgaa gaaatacgcc
    12901 gtctgaaagc gcaacatccc gacattcccg tttatctcgt ggcggaggat atgtgtgcat
    12961 ccggctgcta ctatattgcg gcggcggcag acaaaattta tgcggactcg tccagcatcg
    13021 tcggcagcat cggcgttatc ggcagcagtt ttgatgctac tgaattaatg gaaaaaatcg
    13081 gcgtgaaacg cagggttaaa attgcaggca gcaacaaagg catgggcgat ccgttttcgc
    13141 ccgaaacgcc cgaacagtcg aaaatctggg aagaaatgct gaccggcata cacggcgagt
    13201 tcatcaaagc cgtcaaaacc ggacgcggcg gccgcctcaa attccgacaa tatcccgacg
    13261 tgttcagcgg tcgcgtgtac acaggcgcgg atgcgctgaa agtcgggctg gtggacggac
    13321 tcggcaatat ttacagcgtg gcgcgagacg tggtcaaagc ccccgatgtg gtggattaca
    13381 ctccgaagga cgatttcggc agaatcttgg gcagacgctt cggcgcagaa ctcaaagccg
    13441 gcgtgcgcga agccctgcag gccgtccggt agcgttgttt acagtatttc taagggaata
    13501 ccaaaataaa aatatacaca aaaacactca cctccccagt atgctgccac taagtcagcc
    13561 gcgtcactat ttaccgtgca cttaaagccg taaaaggcag gttgtccccg ggaggttttg
    13621 tgcatcctga taccgatcgg ttgttgttgc tcaaaggaca gaaggccgct gataaacgag
    13681 attacctgtt tgtcgctatt gacgattttt atactctgcc actttgccgg acaaaaccgc
    13741 agacagtgct gccaagtttc tgaccgaaca tctggccgac ccctgcttgt acctgattga
    13801 gtacgtttac tctgacaatg ataggtaata taaagagccg tccaacatgc tttcggtgca
    13861 gcttgttatg ataacgggat tggttggagg cttgcccgat ttgcttgtcc gcagaccaac
    13921 ggtaaggcgg agcgggttat ccgtaccttg atggagatgt ggcatgagga acagtcgttt
    13981 gacagtccgg aacatcggca aaaggagttg tgctgcttta ttaatttagg ggctgcccta
    14041 gatcactagg gaaattcaaa ttaagttaga attatcccta tgagagaaag tcgtctaagc
    14101 cggtaataaa caaaataaac tcattttgtt atcgtggcta tgatgtattg gatgcgggcg
    14161 aatttagcca tttccgtatc aatcacagca cacattttgc cgaacgacaa aaccatatta
    14221 atggaactga gaacttttgg aatcaagcaa aatgtcattt acgcaagttt aacggcattc
    14281 ccaaagcgca ttttgagctg tatttaaagg agtgcgaatg gcgttttaac aacagtgaaa
    14341 taaaagttct tgttccattt taaaacaatt agtaaaatcg agtttatcct agttgtccag
    14401 ggcagcctca tctctttata gatagctgct taccaagtat ccttaattgc taattcaagc
    14461 caatcttcaa aactattaaa aaattcatgg ttttctatat tatctaaatt aatgacaata
    14521 accctagaat ttcctgttac atcattacca tcaaaataag cttcccattc tccattacta
    14581 gcgaatggga taagatttaa atcaggcagg atagaatttc taaatatata agctacttct
    14641 gccccttctg ttccataatt ctcaaactac cagtgaaaat tatgtggctg aatataaggt
    14701 attaaagatg tatcgttaga gatacgaaca tacttttcag gataacaaaa cctttcagga
    14761 agcatattac taggatataa attaaattca ttttgaataa tcatttcgtt accttaggat
    14821 cataaatact accattagga aactgggtag accaatttgg tggtgctagt tcattttcaa
    14881 tttgtttttt aattctaaat tgaatagctt ctccccatgt ttgcggcatc taatgctgct
    14941 tgctctgcat caatattact aaaaattgga ttagtctcaa aaatgaaaaa ttactgtttt
    15001 ttcatttttt atagcatatt caagtacatg aataatattt ttcactaagg gctctgtaga
    15061 aaaatctgaa ttttgtaatt tttcttttac agccaaaata ctttccttat ctattagcca
    15121 atccccctca cttggtccaa ataaccaatt atcggtaatt tcagaaacaa gtctccataa
    15181 tccacgattt tgatattcaa taatttgact ttccgtaatt tctacaactg tttcagattg
    15241 aatgttttta taagcccaat caatcatttc agtatattca ctaattgaaa cattgtcttt
    15301 taaacagaaa attaacaggt tgtgttcatt catttgtcta agttcttaat atgagtttgt
    15361 tgttgtaaat aatcttttgc ttgcttacct tgtaaattaa aggattaacc gcaccttgga
    15421 tgatgccgcg aatatcatcc atacggttag cccaccaatt tttattgggg tcgttttccc
    15481 atatcagttt tcctgcttca tcagcacaat tagggttggc attgatgccc acccagttcc
    15541 agcctgaaaa ggatttttaa tatacggcaa aaggtgggca acaagtgccc accttaccta
    15601 gctaacattt atcccaagta tcatatattt ttttaagttc tttatccaaa gaaagaactt
    15661 tattttttca acaccatgaa ttatctcaaa atctctacaa tccacgcata cgcctgaata
    15721 aatttctcct acaattgctt gaataatata caactcatct ttatcgagac taattttaac
    15781 tgaactctct tcaaaattaa ttaatttcat gaataattcc ttttatttta attcgtcatc
    15841 aaaataccgt ttgcctgttt ttggtctaaa tgctgtccac catctttagt cttggatatt
    15901 tagattataa tcgtcaaatt cccagtcaaa atattttgcc aaatttgata tagacttatc
    15961 ttcagacgtt gaattacata tttccaatat atcatttatc aattttaata tgtgttcaat
    16021 attaaaacgg tttgtaacaa ttgagttttt taatgacatc ggtctatgta tatttgaaag
    16081 taaccattcg gtgctatata tataaacata aaaataatca gcaccattac tttcatcaaa
    16141 acctattgat aatgtcaaaa gtatacaaaa attttctaac ttgtcttgtg ggaaatcttc
    16201 caaatcaaag tcagaacaat cataattttt taattttaat ctcataatat attgagatag
    16261 cttaggaatt ttattttctc agattgaggc aactcatcaa aaccaagcca acaataaaaa
    16321 ttgttatatg attttaaaaa atcaacaatt tttatttttc ctaaaaccaa atcttcgact
    16381 aactgggttg cataaataat atcatttatg attttaacag gtaaattatt tgataggtaa
    16441 ccatgggatt ccgtcatcat tgatttctct aggtgcttat ttatctgatt ggtaagtctt
    16501 ttgcgtaagc ttctttcgcc tccgcaagtg tcatttcatt agggccacca tctatattag
    16561 tttctcctgt attttgccac tgacaaacgt cacagatatc atagtccata acttcagttc
    16621 cgcaaacagg acaatgtagc catatttctc cgtcaatttc ccatgtcttt tttgaattct
    16681 ccatcataat acttctttcc taagtttggt ttaaacattg ttttgatttt gaatgttttt
    16741 ggattggcca aaacatagat attgttattg atatcatagc gcactcgtct atgttccgtc
    16801 tgataaccta atatcttgtc cgatgtctgt tttgataaca aatcagacgc cattttttgg
    16861 tagtcttcaa tggttatatc gccaaattct tttccatgag ctttgaaatg tccatttaaa
    16921 gatttctcag taggaaactt ggactttgtc catctgatgc ggtcttgaat tgttcctgtc
    16981 ggagaaatag atgtatcagg tttaacatat gaggcaggta agccgatatt gttatcagag
    17041 tgtgtagggt gttgctgcgt acttggtttt ggtggcttat tcccacctgc catctcccca
    17101 tctaaagtct gcatagggcg ggcagtaggt gttttatagc cggtattttt aacccaatcc
    17161 catttggtcg ggttaagttc tactttttta ccgccccaaa ccgtacctcc agtcaggtgt
    17221 agttggatgg ggattcattt ttaataatat cttcaagcaa gtgaaatgta agaaaagcgt
    17281 agatacttga aggccggcta tctgattggc aaaaagaaat ttcggagata aagaatttcc
    17341 gtttatattt ttcataaatt aaatcaacac cttgttttac ataaaaacta acttcctcag
    17401 gaaggatatt tataacattc tctcctttta cttttatagt taatggtttg atttttatgt
    17461 tttcatgaat atctgatagg attatttcta aaaaattatt tttatttcca gtcattctgg
    17521 ctattttatg tatatttcca ttgtgttcga ttatcatttt attttttcca aggatttcct
    17581 tctatattga tagtttatct aaagatttca tttaaaatat cctgactaat ttcttgttta
    17641 tataattcta caattttctg tttctgtgta tttgtataaa atttttcgtt ggtatctgct
    17701 tgatatttta attgtctcaa tatttccatg atttcatatt ctgatttttc atcatattct
    17761 tcaatcatgt aatcaacaaa ttgttttaga atatcaaagg cttcgtaact ttccaaaatt
    17821 ttaatatgtt tatcggcaaa attggacata taccattcgt cagtgattgg attagtattt
    17881 aaatcatcta aaaaaacatt tacatcatct aaaaacatga ctaatcctta aacttaaaat
    17941 ctgttttttc accggttctt gtgttttcaa taaacttaaa atctgttttt tcaccggttc
    18001 ttgtgttttc aatgtaatgt atttcaattt tgtttacgtt ttgaacacgc ttaacccaac
    18061 cgtcttttgc taaccaacca tttttagtat cggacatagg aggtattctc gcaggagttt
    18121 tgccctgtgg gtttgcctta acttcttcca ttgctaattg ttcctttaaa ttattaggta
    18181 ttgtacgacc ggttgatttt tcaacagatt gtgtccctat ttttaatgat ttgtttcccc
    18241 cagccatttc cccgtctata gtctgcatat ggcgaacagc aggtttttta tagccggtat
    18301 ttttaaccca atcccacttg gtcgggttaa ggtttacttt tttaccgcct caaaccgtag
    18361 ttgcggcctc tgctatggca agggcggttt gggcagtagc tgtaat
//
LOCUS       DAWFAD010000030        18381 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWFAD010000030 DAWFAD010000000
VERSION     DAWFAD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18381
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.030"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            147..1544
                     /gene="aspA"
                     /locus_tag="WGO49_000752"
     CDS             147..1544
                     /gene="aspA"
                     /locus_tag="WGO49_000752"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ0305883.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFTAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1616..2491)
                     /locus_tag="WGO49_000753"
     CDS             complement(1616..2491)
                     /locus_tag="WGO49_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0305884.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDIGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVEAAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHGGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFSGNLLVAFVLAGVLMFACLFAKSVRWRVESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWAEVKALFGI"
     gene            complement(2493..3185)
                     /locus_tag="WGO49_000754"
     CDS             complement(2493..3185)
                     /locus_tag="WGO49_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEZ0305885.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRTQYDASFRGHEERGRQEEAFRREQARREQFYREQMRREQA
                     LRQAFERQASRSRHAYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVILAYMKRNS
                     LDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYFYRIIAGFIRF
                     NGGRAVAPEKWI"
     gene            complement(3207..3686)
                     /gene="ybaK"
                     /locus_tag="WGO49_000755"
     CDS             complement(3207..3686)
                     /gene="ybaK"
                     /locus_tag="WGO49_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ0305886.1"
                     /translation="MSKQTYPVTPAVRVLRENGIKFEPFIYPYEEHGGTAQFAHLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3762..4124
                     /locus_tag="WGO49_000756"
     CDS             3762..4124
                     /locus_tag="WGO49_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0305887.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKKDAATE"
     gene            4159..5022
                     /locus_tag="WGO49_000757"
     CDS             4159..5022
                     /locus_tag="WGO49_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ0305888.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGDDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVGAEL
                     AASAEPLAAQEPCLAFTAECGSDGICSVVLPHRGILHIAPETALPPYDAAAENAWRLH
                     EILSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEETGIVLDSVKDSESFTALAVIKFSAAQKELTVPNGGIFKA
                     VHPFFKTENAE"
     gene            5120..6079
                     /gene="ttcA"
                     /locus_tag="WGO49_000758"
     CDS             5120..6079
                     /gene="ttcA"
                     /locus_tag="WGO49_000758"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ0305889.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPDDKIIVCLSG
                     GKDSYALLDILRQLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
     gene            complement(6173..9112)
                     /locus_tag="WGO49_000759"
     CDS             complement(6173..9112)
                     /locus_tag="WGO49_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HEZ0305890.1"
                     /translation="MSGFNYEKNQPHQMRAVSAVLGVFDGATPKYRTADENPELLFAA
                     KQYANNILKVQSQNGIDGRFPDRSDDQNILDISMETGTGKTYTYTQTMFELHRWLGVF
                     KFIVVVPTLSIKAGTQQFLQSKALAEHFEQDFGGDYEGVRLKTYVVESAKKNKGKKSN
                     APITIEQFVKAENKKEIHVLLINAGMVNSSSMNDTGDKALKDLFDNPVDALAAVRPFV
                     IVDEPHKFPTRESAKTWGNIKRLKPQYILRYGATFNDEYYNLLYRLTAVDAFNDGLVK
                     GVRVFQEEMQGGMDAAVKLVSSGGKEAKFELNEKDKKQTFKLAKGEDLAQIHPAISDL
                     KIDKMNKTVVVLSNGLELKTGAVINPYSYSQTVQDAMMQRAVAEHFKLERALLAERAP
                     QPKIKPLTLFFIDDIAGYRSGNELSGSLKDKFESWIRVEAARRLKTESDPFYRDYLQK
                     TLDDVSACHGGYFSKDNTDSDDRIEQEINEILHDKEKLLSLDNPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNELMARVRDVPYKLNYFVDSSEKDFV
                     KQLVGEINDNSFQEEISKKFTEELKQKILQKYPDIKPLVLVNQLFSDGIIDDNENFAE
                     DGYDKLKAAYPEAFPKGLDKGKVSNAKDEGKDTIIMREGKYEELKALWELIHHKAVLQ
                     YKIKDEAEFVDLFTAYLRENAAKFPQAGIRTAVNEAYINNGLMLSRRIDSFEDEDFIR
                     FNTMTYRELLEKLAQTAKIRMQTLHQAFYRVRDELNIGDFLNMQTIAQIKNGFNRFLL
                     HHSFHKFELDYRLVGSKIHPTKFTDKDGKPRAVKKADLGRFEDTEHRPAAGYLFDEIF
                     YDSDIEHENVANNQIEGVTVFTKIPRNSIKIPVAGGGTYSPDFAYIVKTKSGEILNFV
                     IEAKGTDGAEDLRKSEERKIKHAEKLFAEISKEIKVVFKTQFDGERIAELIGQNMPAG
                     GHSENGH"
     gene            complement(9125..10816)
                     /locus_tag="WGO49_000760"
     CDS             complement(9125..10816)
                     /locus_tag="WGO49_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0305891.1"
                     /translation="MNWLGKSYARLLRNLPPETLISEDKTHNAKPENAGSQNLLIRGD
                     NLEVLKHLKNAYTNSVKMIYIDPPYNTGSDGFVYQDDRKFTPAELACLANIDEDEAAR
                     ILDFTDKGSNSHSAWLTFMYPRLYIARELLKDDGVIFISIDDNEAAQLKLLCDEVFGE
                     GNFIKDLIVNTSEGGGNAKYVVNGHETVLVYAKDITHFDNLKRPKDIRGKKIVINDEL
                     YWIQEDAVREQFGKYGNLHYEDIIEIKGIEFKNKIDKDIENNEYILIPKSYGKTIIGK
                     LRKVSDDFSKFHSILNIGNISKNLTKDGIRELEELFETGKGYSPFETPKPLDLLKRLV
                     LSATFKGNQTDLILDFFAGSGTTAHAVMQLNAEGQNGNRRYICVQLPEKTAEKSEARK
                     AGYPTIFDITKARIEKAAAKIRVEHPDYTGDSGFKIFQTADNFRQHPDKDFSPEQPDL
                     PLNDELSEEQLQTLLTTWTLYDGAALTTPVEPVRLGAYTAYLCEKRLYLMNAGFTSAD
                     LLAFIRKLDDDAAFNPNRVIVFGSNMASAMQHELDQAVRGYANKKEIELNVVIRV"
     gene            complement(11170..11727)
                     /locus_tag="WGO49_000761"
     CDS             complement(11170..11727)
                     /locus_tag="WGO49_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0305892.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDDTVFHRVIDGFVIQGGGFTEDLVQKATDKAVANESGNGLKN
                     TAGTIAMARTADPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMNTVSKIARV
                     KTATHGLYQNVPVQPVKIRRIVVGQ"
     gene            complement(11792..12718)
                     /locus_tag="WGO49_000762"
     CDS             complement(11792..12718)
                     /locus_tag="WGO49_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0305893.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTPEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            complement(12706..13182)
                     /locus_tag="WGO49_000763"
     CDS             complement(12706..13182)
                     /locus_tag="WGO49_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ0305894.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            complement(13253..13708)
                     /locus_tag="WGO49_000764"
     CDS             complement(13253..13708)
                     /locus_tag="WGO49_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ0305895.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            complement(13833..14948)
                     /locus_tag="WGO49_000765"
     CDS             complement(13833..14948)
                     /locus_tag="WGO49_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ0305896.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNALP
                     ACGQSLLNLASVSTENRYKIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIVGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            complement(15062..16159)
                     /locus_tag="WGO49_000766"
     CDS             complement(15062..16159)
                     /locus_tag="WGO49_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ0305897.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEGLFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKAGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIGVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            complement(16241..17824)
                     /locus_tag="WGO49_000767"
     CDS             complement(16241..17824)
                     /locus_tag="WGO49_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ0305898.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTVDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     KHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNTYYVAHEYMEIFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEAFKTEDVAENLARRFPGLTF
                     DRMKINSQLLLQTILGRFSVSSDNAGKQFFEDHKTYVPARFTDYVAAFVEHGAEAFVQ
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            complement(17972..18184)
                     /locus_tag="WGO49_000768"
     CDS             complement(17972..18184)
                     /locus_tag="WGO49_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ0305899.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
BASE COUNT         4347 a         4850 c         4425 g         4759 t
ORIGIN      
        1 ataacacccg aaaacaacgt ttcccgtttt cagacggcat acgatatgga caatttcatc
       61 cggccggtat agtgttcgtc ctgcggcggc atctttccga tggcgcattt tttcaatatt
      121 aattatccga cttacaaagg taaaacatga ctgtccgtat cgaacacgat ttattgggcg
      181 accgcgagat tcccgccgaa gtgtattggg gcatccacac tctgcgcgcc attgaaaact
      241 ttaaaatctc cacacaaaaa atttccgacg tgccgcagtt tgtccgcagt atggtgatgg
      301 tgaaaaaagc aaccgcgcag gcaaacggcg aattgggtgc agtaaaaccc gaaatcgccg
      361 ccgccattga aaaggcttgc gacgaagttc tgctgaacaa ccgctgcctc gaccaattcc
      421 cctccgacgt gtatcagggc ggtgcgggaa cttcggtcaa tatgaacacc aacgaagtca
      481 tcgccaacct cgcattggaa gccttgggat acgaaaaagg ccgctacgac atcgtcaatc
      541 cgatggatca tgtcaatgcc agccaatcga ccaacgatgc ctatcccacg ggcttccgcc
      601 ttgccgtgta ttacagcatc ggcgaattgc tcgacaaact gaccgtcctg aaaaacgcct
      661 ttaccgccaa agccgaagaa tttaaagacg ttttgaaaat gggtcgcacc cagcttcaag
      721 atgccgtacc gatgacggca ggacaagaat tccaatcttt ccaagtgttg ttggaagaag
      781 aaatcctcaa cctcgaccgc acgcgccaac tgcttttaga agtcaatttg ggcgcaacgg
      841 caatcggcac gggcgtgaac acgcccaaag gctacgccga actggtggtc aaaaaactct
      901 ccgaagtcag cggcttgcct tgcaaactga ccgaaaacct gattgaggcg acctccgact
      961 gcggcgcata tgtgatggta cacggcgcat tgaagcgcac ggcggtcaaa ctctccaaaa
     1021 tctgcaacga cttgcgcctg ctctcttccg gcccgcgtgc cggcttgaaa gaaatcaacc
     1081 tgcccgaatt acaggccggt tcttccatta tgcctgccaa agtcaatccc gtgattcccg
     1141 aagtggtcaa ccaagtctgc ttcaaagtca tcggcaacga cacgacgatt accttcgccg
     1201 ccgaagccgg gcaactgcaa ttaaacgtga tggagccggt catcgcccaa tgtatgtttg
     1261 aaaccatttc cctcttgggc aatgccgcag tcaacctgtc cgacaaatgc gtcaaaggca
     1321 ttacggtcaa ccgcgaaatc tgcgaacgtt acgttttcaa ctccatcggt ttggtgactt
     1381 atctgaatcc gtatatcgga caccacaacg gcgatttggt cggcaaaatc tgcgcccaaa
     1441 ccggcaaagg cgtgcgcgag gttgtcttgg aacgcgggct gttgagcgaa gaagaactca
     1501 accgcatcct ctctcccgag aacctgatga acccccatct gtaacgttgc agacaaatgc
     1561 cgtctgaagc agggatgctg cttcagacgg cattttgtta tatcaatcag ccggattata
     1621 tgccgaacag tgccttgacc tccgcccaat aaaacaggca ggcgatggaa cacagtgcca
     1681 acacgattcc cgccgtattg attgtgttga ttttttcctt aaaaaacagt gcgcccgaaa
     1741 gcgtccccaa aacaatcacg ccgatattca taccggcaaa aaccagcgtg ggattgtcct
     1801 tcatcatttg gtgcgcggtg atgtaggtta cgatgttcat gaagttcaaa ccgcccaaga
     1861 gtatgccgcc gaccacactc tcaacgcgcc atctgaccga tttggcaaac aggcaggcaa
     1921 acatcagcac acctgccagc acaaatgcaa ccagcaggtt gcctgaaaat gccgtgccgc
     1981 ttttggcaag ctgtttgaac aggatgtcga taatgccgta acccgcccac acgcccagca
     2041 gcaatgccgc ctgccgccac gcgctgcctg attttttgcc gccgccgtgt ttccaaagca
     2101 ggcagaacag cgcggcaaat gcgaggcaca gcccgattag tttgccttcg ctgagttttt
     2161 cgccaaacag cgtcaaggcg gcaacaatcg gcaaaaacag cgacaaacgc tgcgccgcgt
     2221 cggatttgac gataccggcg gcttccacag atttgcccat tatcacgaat acggacggca
     2281 gcagcacgcc caaagcggca aacagcggcc acgtcggcaa aaatgcgccg atattgccga
     2341 tatccggctt caataccagc agggtcagta tgaccgcgac cacataattg acggcgaccg
     2401 cctgcgcgat gtcgattttt ttcttccttg ccattttcag caaaatggaa accgacacgc
     2461 tgaacacgat gctgattaac aggtaagcca tactatatcc atttctcggg tgcaaccgcc
     2521 ctgccgccgt tgaagcggat aaagccggcg atgatgcggt agaaatacca gacgttcgtt
     2581 gcaataataa tcagcacgcc gatacccaaa agggcagtca gcgcacccaa aatataaagc
     2641 caaaatgtgc gccaaaaggt tttaatcagg tattcggtat gtgcggcata gacaatgctg
     2701 tccaaactgt tccgtttcat ataggcgaga atcacgccga ctatgggcat gaacagcatg
     2761 attgcaccca aaccgaacag gatgtaggcg gcgaggacat agttgcgccc gcttccgccg
     2821 ccggacggtt cgtaagcatg gcgcgaacgt gaggcctgcc gttcaaacgc ctgtcttaac
     2881 gcctgttcgc ggcgcatctg ttcgcggtaa aactgctccc tgcgcgcctg ttcacggcgg
     2941 aatgcctctt cctgccgccc gcgttcctca tgccctctga aagacgcgtc gtattgcgtc
     3001 cgtttcgaca ggtcggaaag cgtatcgtaa gcccgttgga tttctttgaa cttttcttcc
     3061 gcctgcggat tacccggatt gcggtcggga tggtatttca tcgccagctt gcggtaggcg
     3121 cgtttgattt cgtccgcccc cgcctgcggc gacacgccca atacggcata caaatctttg
     3181 tccatattgc tccttatact tccccgtcaa accgctgcct gtattgtttt cgggttcaaa
     3241 atatttaaat ctccgggacg gatgccgata atgaacccgc gttttccgcc gttgatatag
     3301 atggtttcca aatccatcac cgactgttcg acgtaaatat ccaattttgt ccggatgccg
     3361 aacggcgttg tgccgccgac cagatagccc gtccacttgt ttgcctgtgc gggcgtggcg
     3421 ggttcgatgt gtttcgcacc caaatgccgc gccagattgc gggttgaaat ctgtttgtcg
     3481 ccgtgcatca agacaatcag ccccttaccg ttttcatctt gcaaaacaat ggttttaata
     3541 accaagtgtt cgtctttgcc gaacaggtgc gcaaattggg ccgtaccgcc gtgttcctcg
     3601 tagggataga tgaaaggttc gaacttgatg ccgttttcac gcaaaacgcg tacggcaggt
     3661 gttaccggat aggtctgttt actcattttt cgtcatgcaa aacatggaaa tcacccaaat
     3721 gcaatgttac catatatccc cctttattga aggaaaccga tatggatatc cgatatttcg
     3781 gcacaacgcc ccgctattcc gaagccgtcg gcgcaaacgg cttgattttc ctctccggca
     3841 tggttcccga aaacggtgaa acggctgccg aacagactgc cgacgtgctt gcccaaatcg
     3901 accgctggct ggcggaatgc ggctcggaca aggcacacgt tttagatgcg gtcatctatc
     3961 tgcgcgatat gggcgactac gcggaaatga acggcgtgtg ggacgcttgg gttgccgccg
     4021 gcagaacgcc tgcccgcgcc tgcgtggaag ctcggctcgc ccgtccggag tggcgcgtcg
     4081 aaatcaaaat aaccgccgtg aagaaagacg cggcaaccga ataaacagat ttcctttata
     4141 tttcagagta cgcaaatgat gaaaacctta ctgccctttt tcggcgtggt gcgcgtcagc
     4201 ggcgaagacc gccaaacctt cctgcacgga cagctttcca acgacatcaa taatcttcag
     4261 accgggcagg cgtgttatgc gacttacaac acgcccaaag gccgcgtcat cgccaatatg
     4321 attgtcgtca accgtggaga cgatttgctg ctgattatgg cgcaagactt gcttgaagca
     4381 acggtcaaac gtttgcggat gtttgtgttg cgtgccaaag ccgtttttga aatccttgaa
     4441 gattacgccg tcggcgcgga attggcagca tccgccgaac cgcttgccgc gcaagaaccc
     4501 tgccttgcct ttaccgccga atgcggttca gacggcatct gttccgtcgt cctgccccat
     4561 cggggcattt tgcacatcgc ccccgaaacc gccctgccgc cttatgatgc cgccgccgaa
     4621 aacgcgtggc ggctgcacga aatcctcagc ggttatccgt ggatatgcgc ggctaccaaa
     4681 gaaactgccg ttgcccaaat gctcaaccag cacatcatcg gcggcgttca cttcaaaaaa
     4741 ggctgctacc cgggacagga aatcattgcc cgtgcccagt atcgggggca ggtcaaacgc
     4801 ggcttggcgg tattgtcggg aaattcggcc gtcgaagcag gaaccctgct gacggcagac
     4861 ggcgaagaga ccggcatcgt gctcgacagc gttaaagatt cggaaagctt taccgccctt
     4921 gccgtgatta aattctccgc cgcacaaaaa gaactgaccg tcccaaacgg cggtatcttc
     4981 aaagcggttc atcccttctt taaaaccgaa aatgcagaat aatccctcta atgccgtctg
     5041 aacctttttc agacggcatt ttaaaatcgc ccctgttttc ggttacaatg ccgcatccct
     5101 aatttttcaa accaccgcca tgtccaaaaa aaccaaacaa gaactcgaaa acaacaaact
     5161 cagcaaacgc ctgcgccacg ccgtcggcga cgcgattaac gatttcaata tgatcgagcc
     5221 ggacgacaaa atcatagtct gcctctccgg cggcaaagac agctatgccc tgttagacat
     5281 cctgcgccag ctccaagcca gcgcgccgat tgattttgaa ctggtcgccg tcaatctcga
     5341 ccaaaagcag ccgggtttcc ccgaagaagt attgccgacc tatctcgaaa gcataggcgt
     5401 gccgtacaaa atcgtcgaag aagacaccta ctccaccgtc aaacgcgtat tagacgaagg
     5461 caaaacgact tgttcgctgt gcagccgcct gcgccgcggc atcctctacc gcaccgcaaa
     5521 agaattgggc tgcaccaaaa tcgccttagg acaccaccgt gacgacatcc ttgccaccat
     5581 gtttttaaat atgttttacg gtggaaaact caaagccatg ccgcccaagc tggtgagcga
     5641 caacggcgaa cacatcgtca tccgtccgct ggcgtatgta aaagaaaaag acttaatcaa
     5701 atacgccgaa ttaaaacaat tccccatcat cccgtgcaac ctctgcggct cgcaacccaa
     5761 cctgcaacgc caagtcatcg gcgatatgct gcgcgattgg gacaaacgct ttcccggccg
     5821 catcgaatcg atgttctccg ccctgcaaaa cgtcgtccct tcccaccttg ccgataccga
     5881 actcttcgac tttgccggat tggaacgcgg tcaaaccctg aaacacggcg gcgatttggc
     5941 gtttgacagc gaaaaaatgc ccgaacgttt ttcagacggc agcgaagaag acgaaagcga
     6001 aatcaaaatc gcgccgcaaa aagccgaacg caaagtcatc aacattctgg cgaacaagcc
     6061 gaaaacctgc ggcgcgtaac cccccgccgt caaaaatgcc gtctgaagcc cgtttggcgt
     6121 ttcagacggc attttaaaat cccgaagcag acggcttcca aacatttgtt tctcagtgtc
     6181 cgttttcaga atgcccgcct gcgggcatat tttgcccgat cagttcggct atcctctcgc
     6241 cgtcaaactg cgttttgaac accaccttga tttctttgga aatctcggca aacagctttt
     6301 cggcatgttt gattttccgc tcttcgcttt ttcgcaaatc ttccgccccg tcagtccctt
     6361 tggcttcaat cacaaagttc agaatctcgc cgcttttggt tttcacgata taggcaaaat
     6421 cgggcgaata cgtgccgccg ccggcaacgg ggattttgat ggagtttctc ggtattttgg
     6481 tgaatacggt tacgccttca atttggttgt tggcgacatt ttcatgttct atatccgaat
     6541 cgtagaaaat ttcgtcgaag agatagccgg cggcaggccg gtgctccgta tcttcaaatc
     6601 tgcccaaatc tgcttttttc accgcgcgcg gtttgccgtc tttatcggta aatttggtcg
     6661 gatggatttt gctgccgaca agccggtaat ccagttcaaa tttatggaag gaatgatgaa
     6721 gcaaaaaccg gttgaagccg tttttgattt gggcgatggt ctgcatattc aaaaaatcgc
     6781 caatgttcag ttcgtcgcgg acgcgataaa acgcctgatg caaagtctgc atgcggattt
     6841 ttgccgtttg tgccagtttt tccagaagct cacggtaagt cattgtgtta aaacggataa
     6901 aatcttcatc ttcaaaactg tctatgcggc gggaaagcat aagcccgttg ttgatataag
     6961 cttcgtttac cgccgtgcgt atgcctgcct gcgggaattt ggcggcgttt tcacgcaaat
     7021 aggcggtaaa taaatcgaca aattcggctt catccttgat tttgtactgc aaaacggctt
     7081 tatggtgaat cagctcccac aaggctttga gttcttcata tttgccttcg cgcatgatga
     7141 tggtgtcttt gccctcgtct ttggcgttgc tgactttgcc tttgtccaaa cctttgggga
     7201 aggcttcggg ataggcggct tttaatttgt catagccgtc ttcggcaaag ttttcattgt
     7261 cgtcaatgat gccgtctgaa aacagttggt ttaccaatac cagcggtttg atatcggggt
     7321 atttttgcaa tattttttgt ttcagctctt cggtaaactt tttggagatt tcttcctgaa
     7381 aagaattgtc gttgatttcg ccgacaagct gcttcacaaa gtctttttcg ctgctatcga
     7441 caaaataatt cagtttgtac ggtacatcgc gcacccgcgc catcagctcg tttaccggca
     7501 ggcgcaggcc gcgtccgact tcttgcagct tggaagtcgt gctgccgctg gaacgcagtt
     7561 tgcaaatctg gaaaacgttg ggattgtccc agccttcgcg cagcgtccat ttggaaaaaa
     7621 taaagcggcg cgggttgtcc aaagacagca gtttttcctt atcgtgcagg atttcattga
     7681 tttcctgctc gattctatcg tcgctgtctg tattgtcttt ggaaaaatag ccgccgtggc
     7741 aggcggatac atcgtccaac gtcttttgca ggtaatcgcg gtaaaacggg tcgctttccg
     7801 ttttcagacg gcgtgccgct tccacgcgaa tccagctttc aaatttatct ttcaggctgc
     7861 ctgaaagctc gttgccgctg cggtagcccg cgatatcgtc aataaaaaac agcgtcagcg
     7921 gcttgatttt gggctgtggc gcgcgttctg ccaaaagcgc gcgttccagc ttgaaatgtt
     7981 cggcaaccgc ccgctgcatc atcgcatcct gcaccgtttg cgaataggaa taagggttga
     8041 tgacggcacc cgttttcaac tccaagccgt tgcttaacac caccacggtt ttattcattt
     8101 tgtcgatttt caaatccgaa atagccggat ggatttgcgc caaatcttcg ccttttgcca
     8161 gtttgaacgt ctgctttttg tccttttcgt ttaattcaaa tttcgcttct ttgccgcccg
     8221 acgacaccag ttttaccgcc gcatccatgc cgccctgcat ttcttcctga aacacgcgca
     8281 cgcctttgac cagcccgtcg ttaaacgcgt ctactgccgt caaacggtaa agcaagttgt
     8341 aatattcatc gttaaatgtc gcaccgtagc gcaaaatata ttgcggtttt aagcgtttga
     8401 tattgcccca cgttttcgcg ctctcccggg tcgggaattt atgcggttcg tccacaatca
     8461 caaacgggcg cacggcggcc aatgcatcaa cgggattgtc aaacaaatct ttcaatgcct
     8521 tgtcgcccgt atcgttcatg gacgacgaat taaccatgcc cgcgttaatc agcagcacat
     8581 ggatttcctt tttgttttcc gctttgacaa attgctcaat cgttatgggc gcattggact
     8641 ttttgccctt attctttttc gcgctttcca ccacataggt tttcaggcgc acgccttcat
     8701 aatcgccgcc gaaatcctgt tcaaaatgct ctgccaaagc cttgctttgc aaaaactgct
     8761 gtgttcccgc cttaatggac aaagtcggca cgaccacgat aaatttgaac acgcccagcc
     8821 aacggtgcag ctcgaacatg gtttgtgtgt aggtataggt tttgcccgtg cccgtttcca
     8881 tggaaatatc aaggatattt tggtcgtccg aacggtcggg gaatcggccg tctataccgt
     8941 tttggctttg cactttcagg atattgtttg cgtattgttt tgcagcaaac aaaagttcgg
     9001 gattttcgtc tgccgtccga tatttgggcg ttgccccgtc aaacacgccc aaaaccgccg
     9061 aaaccgcccg catttggtgc ggctggtttt tctcgtaatt aaaaccgctc atgaattgcc
     9121 tccgtcaaac cctgataacc acattcaact caatctcttt tttattggca taaccgcgaa
     9181 ccgcctggtc aagttcgtgc tgcatggcgc ttgccatatt gctgccgaat acaatcacgc
     9241 ggttgggatt gaaagccgca tcgtcgtcca gcttgcggat aaacgccaac aaatcggcag
     9301 aagtaaaacc ggcattcatc agatacagcc gtttttcgca cagatacgcc gtgtaagccc
     9361 ctaaccgcac aggctcaacc ggcgtggtca gtgccgcccc gtcatacagc gtccaggtgg
     9421 tcagaagcgt ttgcagctgt tcttcgctta attcatcgtt aagcggcaaa tccggttgtt
     9481 cgggcgaaaa atccttgtcc ggatgctgcc tgaaattgtc tgccgtttga aagattttga
     9541 agcccgaatc gcccgtgtaa tcgggatgtt cgacgcggat tttggcggcg gctttttcta
     9601 tgcgggcttt ggtgatgtcg aagatggtcg ggtagcctgc tttacgggct tcggattttt
     9661 cagcggtttt ttcgggaagc tgtacacaga tatagcggcg gttaccgttt tgtccttcgg
     9721 cgttaagctg catcacggcg tgggcggttg tgccgctgcc tgcgaagaag tctaggatta
     9781 ggtcagtttg atttcccttg aaagttgcag aaagaactaa tcttttcaat aaatctaatg
     9841 gttttggtgt ttcaaaagga gaataacctt ttccagtttc aaacaactct tctaattcac
     9901 gaataccatc tttagtaaga tttttactta tatttccaat atttaagatg ctatgaaatt
     9961 tggaaaaatc atcggatact tttctaagtt ttccaataat tgttttccca taagacttcg
    10021 gaattaagat atattcatta ttttcaatat ctttatcaat tttgttttta aactcaattc
    10081 cttttatttc aataatatct tcgtaatgca agttcccata tttgccaaac tgttcgcgaa
    10141 cggcatcttc ctgtatccaa tataattcat cattgattac aatttttttg ccgcgtatat
    10201 cttttggtct ttttagattg tcaaaatgag taatatcttt cgcataaact aaaacagttt
    10261 catgcccatt tacaacatat tttgcattac caccaccttc tgatgtatta acaattaaat
    10321 ctttaataaa attcccttcc ccaaacactt catcacacaa caacttcaac tgcgccgctt
    10381 cgttatcgtc aatcgagata aaaatcacgc cgtcgtcctt taacagttcg cgggcgatat
    10441 acaggcgcgg atacataaag gtcaaccatg cactgtgcga gtttgagcct ttgtcggtga
    10501 aatctaaaat ccgcgcggct tcgtcttcgt caatattggc taagcaggca agttcggctg
    10561 gtgtgaattt gcggtcgtcc tgatagacaa agccgtctga tccggtgttg tagggcgggt
    10621 cgatgtaaat catcttcacg ctgtttgtgt aggcgttttt taagtgtttc aacacttcta
    10681 gattgtcgcc acgaatcagt aggttttggc tgcctgcatt ttcgggtttg gcgttgtgcg
    10741 tcttgtcttc acttatcagg gtttcgggcg gcaggttgcg aagcaggcga gcatatgatt
    10801 tgcccagcca gttcatttcg taaaattcgc gtccgatgtc ggtctgtggc gcaatttcgg
    10861 cttgtaatct gtcgataagg aaatttccgt ctgcgtcaaa acaggcggga aacagttttt
    10921 tgagctgttc gagttgggta gagttggcgg taatgccgtc tgaagtgtag attgcctcgg
    10981 tgttcacccc ggctgtgtcg attagatcag gggcttgggt tgggttgggt tgggttgggt
    11041 tgggttggca gcattttaaa atcctcggtt tgaatttgtc aatatcaact atctgtttta
    11101 aaatattttt tcacttcaga cggcgttttt tgtgaaacgg gcgacgccgt ctgaacatct
    11161 gtctgcgtgt tactgtccga caacaatgcg acggattttg acgggctgta cgggtacgtt
    11221 ttgataaagg ccgtgcgtgg cggttttgac gcgggcgatt ttggaaacgg tgttcatgcc
    11281 gctttcgacc ctgccgaaaa cggtatagcc gtattgtccg tttttgtagt tgagtgaatc
    11341 attgtccgcc agattgataa agaattggct ggtggcggaa tccggatcgg ccgtccgcgc
    11401 catggcgatg gtaccggcag tgtttttcaa gccgttaccg gattcgttgg caacggcctt
    11461 atcggttgcc ttttgtacca agtcttcggt aaatccgccg ccctggataa cgaaaccgtc
    11521 gataacgcgg tgaaaaacgg tgtcgtcgta aaagcctttt cgggcatagc gcacgaaatt
    11581 agcaacggtt ttgggggctt tggactcgtc caaaaccaaa cggatattgc ccatatcggt
    11641 ttccatcaaa acatgggttg ccgccataga cggcagggaa accgccaaaa gcagcgcggt
    11701 taaaacggtt ttgaatttgg gtttcatccc gtcctcctca gaccttcaga cggcattttc
    11761 atttcatatg ccgtctgaag gcgcgtgaac gctattccaa tgcgtctttg agcttttgtt
    11821 cgattaaatc cgcatcaaac gatttggcaa tcaattcaaa acgcgaatcg cgccgccatg
    11881 agacttggtt cgcgccccac tgtccgtcta cccagttgag ccacacccac gttcccaata
    11941 cttgaaacac gcctttggcg cggacgagtc cttcggtcat tttgggcagg tcgttgaaaa
    12001 agttggtcag cttttcgccg tcgaaatcgc gtccggcggg gaatgtgaaa ccttgcgact
    12061 ggaagcccat ggtattatcc ggcagggctt tgaggcggta gcgtgatttt tcgatgacgg
    12121 gaatatcgag ccaccggatg tcgagttgtg cgttttggac ttcaaccact ttggctttgg
    12181 gcgggaacag ttttgccgct ttgtcgtgaa attcggcaag ctgttcgggg gtgcataaat
    12241 cggttttgct ggcgaccaat acgtcgcaga tgccgatttg gtctttatac aacgcctgtt
    12301 gcgcgtaatc ggggttgatg aactggcgcg gattgacgac ggtaaagacg gcgccgattt
    12361 ccaaaaggct gtccagcggt ttggctttga gttcgtcgat gacgctggct gcgtgagcca
    12421 gtccgcttgc ttcaatcatc aggcggtcgg gcttggcgtc gcgcagcatt ttctgcacgg
    12481 ttacgcccat ttgcgggccg gcggtgcaac acaaacagcc gccggcgatt tctgccacag
    12541 ggataccgtt gtcgctcaat accgcgccgt caatgccgat ttcgccgaac tcgttgacga
    12601 tgatgaccca tttttcgttc gggtcttttt gcgccatcag gcttttgagc gcagtggttt
    12661 tgcctgttcc cagaaaacct gaaatcaggt ggactttggt ttttttcatt tctgacattt
    12721 tttacagatt ccagttaaaa caacgtgttc ttctttcagc gcaaagccgc tttcggcaac
    12781 gcctgcgcgc agtgccgccc actcgtggct gagggtttgc tcgtccgccg tgccgcattc
    12841 ggtgcagacc aaaataaacg cgctgtggtg cgcttcggct tcttcgtggt cgtggcaatg
    12901 gtcgtcgcac tcgtgctgcg cgtggctgca caaaatatag ccgttgaccg ccgccacttt
    12961 gtgcaaaacg ccctgctccg cccaaaaatc aagggcgcgg taggcggtag gcggtgcaag
    13021 cacgccctcg ctttgctgct gcatctgcga caagacgttg taggctttaa tcacgccgct
    13081 ttgctgcaag acaatatcta atacctgctc gcgcaaagcg gttacctgca agccttcgct
    13141 gcgtgcctgt tcgataattt tctgtttgaa atttgttttc ataaattctc tgtttatgcc
    13201 gtctgaacaa ccgatacggc aggaggcggt tttatatttg tattcaattg ctttatttgg
    13261 aaatcttttc caacaatgcc cgacagcccg catccgcaag cctgaaggtt tcttcaaaat
    13321 cgcccgtata ccacggatcg gggacatggt cgtaaccgct ttcgggtatc aggtcggtca
    13381 gcttgaatat tttttccggc cgcctgccga aggttctttc caattcggac aaattcttgc
    13441 cgtccatcgc gatgatgcag tcaaacgccg ccgcatcgct ttggcggatt ttgcggctgg
    13501 taaagcctga agcatcgata ccgtgttttt tcaatatctt tgccgtctcg cggtgcatat
    13561 cttcgccgtc gtgccagccc gatgtccccg cgctgtccgc ttcaagggga atgcccgctt
    13621 cggcggcgcg gcggcgcaaa atgtattccg ccatcggcga acggcagatg ttgccgaggc
    13681 agacaaaaag gattttcggt tttttcatat cccctccctg ttccggcgcg atgccgtctg
    13741 aagcggaaac cctttcagac gtcatcggtc ggttaatcgt aaccgcataa ggcagaacca
    13801 gccaaaacat ccatctggaa gaaatggttt ggctaaatct taggcatatt taataagtgt
    13861 ccaatattag aagccgtatg ctccaaatag agactggcat ttttcaaact atcttctaaa
    13921 ggttcacttt tctccaaaat agaaaaggca gcttggatat tttcaaatgg cagggaaggc
    13981 aaatcttcaa cgagactgcc acaaatagcg acaacaggaa ctccgacagg ggttcttttt
    14041 gctacaccaa taggcgcttt ccctgctaaa ctttgacgat ctagtcttcc ttcaccaacg
    14101 ataaccaagt caacatctga cactttctta tcaaagtcaa tcaagtccag gcaggtgtca
    14161 attccagata cgatacttgc ctgagcaaag gcgcacaaac caccagcgat gcctccacca
    14221 gctcctgctc ctttaagttt taatgttgca ggggagactt tttcataaaa atcttgtatt
    14281 gcctgatcta cggcctcaaa catagtagaa tccaaccctt tttgcttgcc aaacgtatag
    14341 gtcgcacctt ggtgtccaca taagggactc acaacatctg ctaaaatacg aatgtgaaca
    14401 tcttcaggaa ttttatagcg attttctgtt gaaacagaag ctaggtttag taaggattga
    14461 ccgcaagcgg gtaaggcatt tccatcctca tcataaaatt gataacctaa accagcagca
    14521 atcccaatac ctccatcatt actggccgta ccgccaacgc caatatagat ttctttaatt
    14581 tcttgactaa tgaggtggcg aatcaattct ccaataccac gagtttggat ttgtaatgga
    14641 tttcgtttct ctagcgggat ttttccaaga ccaaccaaat cagcaacttc gaatagggct
    14701 agttgttctt tttgaaaata gcgcatgact tctttttgtc caaaaggtcc tgtcacttgg
    14761 agacattttt cttctaggtc aagagaatgt cggatagcat ctacagtgcc ttctccccca
    14821 tcaccgacag gacagaggag acattccaca tctgctatcg attgttggaa gcctcttttt
    14881 attgcttcag ctacctgttg agctgtcaag ctttccttaa acgaatccgg tgcaattaca
    14941 atcttcatat ttataattca tcctttcgtt tcactcaagg cacaacacag aatgaaaaag
    15001 tgttgtgctc tttattttga tttattatag aaatgagaaa gcctatcact actacaaatc
    15061 actatgcgct gaaaaacgga ttgtgccctt cccgtttcaa tgcttccgca tagctcggga
    15121 tgctttcctg ttcgcccaag ggattgtgca acaggtaaac gtgttctaca cggcattctg
    15181 ccattaaatc caaaaaattt tgctgaataa cgccaatatt gtcggtaaag gaaagctgcc
    15241 acgaaaacac ctgcggcacg acttcaaacg cgccgtcggt cgcattaaac ccgaataaaa
    15301 tctgtccgcc cgcctttgcc gctgcgacca cgccgacgcg cggactctgc atacggacgg
    15361 cgcgtatcgc gtttgccgca aacacatcca tcgccatgcg ctggcgcgtg tggctgccgt
    15421 cggaaagcgc gcgtaccgga gaatccgggg aaagcggatt ggcaaacagc gtaacgcctt
    15481 ccgatgcaag attttcctgc catacctgca tcaagggcag acctgcctga agaaggttct
    15541 gacccaatgc cgtctgaact ttgccgctgc cgaaacccag cgcgtaaacg acgtggacgg
    15601 actgcccttc gggcaaaagc aaagggcgcg gggcgaaacg gcgtaagtgt tgcgccgccg
    15661 cttcggtatt ttgtttggca cgccaccact catccggcgc gaccgcactc aaatcggagg
    15721 aatgtacaag ataaggcagc cattgcgtct cttgcgtcaa cgcgcgcagg tgcggatagt
    15781 tttgcaaaca ggcaaacaat ccctccgtcg gcagcttcat ctcgattgcc cgttctttct
    15841 tgcagccgga caccagcacg accggcaggg cgaaccactg cacttcgcct tctttctcgc
    15901 aatcgagtac cgcgttcaca ctggaaagca gcgcggcata agttccggca tcgggcgaca
    15961 tcgtcagcgc gagggaaagg ttgatatagt ggttttgctc aagcatcccc ctgatttcgg
    16021 tttgaagttg gccggacgag agtttgcgcg aagcctggga agaattatgc gccaactggt
    16081 aggcattgag cagcaggtgg tttttaatcg gattttgggg atacgggcgc gtatcgggca
    16141 aggtaaatgt ctggttcata tatgtcgtgt accgcttttc gcagtgtgtg tttttcctgt
    16201 ttgaaaatat atcggacgga ttgccgtcgg gacttgcccg tcaatccgcc gaaacgagaa
    16261 aatgcctgtc tgccaagtct gccaatattt cttccacata cacttcggca ggcggatgga
    16321 atgtcaaacc gtcgggcgtg gtgctgacga tgttcaagtt ttcggcaacc gtttcaatca
    16381 atgcctgttt gctcgtcggc cgcgacaatt gccgcataat gtacaaatgc ccgtacccga
    16441 atgagggggt gctttcgttg taacggttgg caggctggac aaacgcttcc gcgccgtgtt
    16501 ccacaaaggc ggcgacataa tctgtaaaac gcgccggcac ataggtttta tggtcttcaa
    16561 aaaattgttt gcccgcattg tctgatgaaa cagaaaaacg cccgagaatg gtttgcaata
    16621 aaagctggga attgattttc atacggtcga atgtcaaacc gggaaatcgg cgggcgagat
    16681 tttccgcaac atcttccgtt ttaaacgcct ctccggtttt catcacatcg cgtatgcccg
    16741 aaagcagggt atcgttttca tcaaagttga ttgtttcccc tcttgccggg cggaaattca
    16801 aactttctat cacttcgacg gcaaccgact cgtcacgcct gacagtatcc ccgacttcct
    16861 cacggcataa aagcgaacgg cggaattggc ggtcggataa aatatcgctg taaaattctt
    16921 tggcaatata atcgccccct gccaatgcca gaatccgctc ccgcgtatgc tccgccatcc
    16981 aagaaacaaa agacgcgtgc aaatcggtat ccccgatata cgccagccgg tggcggtttg
    17041 cccattcgat gaagccgtta acgtaaatcg ggtcgttgaa tatttccata tattcgtgtg
    17101 cgacataata agtattgtga ttcaatattt tttgaatcgc cggaagtttg ccgccgccgc
    17161 ccaagccttt gtcgttttcc aaaatttccg ccagcgcctt gagcgcgtcc aagcctttcc
    17221 gcgtccgcgc ttccaagggt tcttcaagca catccctgcc ggcaaagtac ataatttcgc
    17281 gcaactgctc ctgccgtttc cagccagggt aaacattgta tgaaatatag gcaatgccgt
    17341 gtttggtcag gttgttccgg caaatcgaaa agattttgtc tttaactgcg tcaggcaccc
    17401 acgaccaaat gccgtggacg atgatatagt cgaacttccc gaatgactca tcgacggtca
    17461 aaatatcttt ttcttccaga cgcacatttt tcaagcccat tttttcaatg atggcgttgc
    17521 cctgcgcaac ctgcctgccg gacaagtcga taccgacaaa ttccgcatcc gggtaataaa
    17581 gagcctgcgt gatgatgttg ccgcccatcg aacagcccag ctccaagacc ttggcattgg
    17641 cggcgggcgc gggctgcaaa cccatcatgc gggcgcgagc ctccaaggcg ttgatggcgg
    17701 tttgggaaaa tgctttggat tcatacatca aatcatcata tgaatttttg atgttggaca
    17761 cgtccggcac accgttctcc gttgcagcac gcgcaaaggc ggcttttttc tttttgttcg
    17821 gcatattttg tttgtctgaa ggcactattg cccgcaagtt taaccaattc atcctacccg
    17881 ttcaactaaa tcaaatgcca tctgaaggcg cggagcgtac ttcagacggc atcggggagg
    17941 cgcgaaggct tcagacggca tttttgccgc tttatttcaa cgcggcatca tagccgccgt
    18001 cttcaacggc ttcaatcaac gcccctgcat cggtttgcgc ggggtcgtat ccgacggtcg
    18061 cacttttgtt ttcaaggctg acttcgacgc ttgccacgcc ttttacgcct tccaatatcc
    18121 gggtaacgct tttgacgcag ccgccgcagc tcatgccgcc gatgtcgagg ataagggttt
    18181 ccatcatttt cctttcgttg gaactgcaat ctgacgggcg ttattgtaag tcggggcgtg
    18241 aacttgggca aacgcggaaa cggtacggcg gtttggaaaa agacggacgc ttgcgcataa
    18301 tggcggcaat tcccatcagg acgacaacaa tgaacgcttc acaaaaaccc tggttgagca
    18361 tcatcgcctt ggcaatcggc g
//
LOCUS       DAWFAD010000031        17345 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWFAD010000031 DAWFAD010000000
VERSION     DAWFAD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17345
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.031"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..190)
                     /locus_tag="WGO49_000769"
     CDS             complement(<1..190)
                     /locus_tag="WGO49_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305900.1"
                     /translation="MPSERLSDGIFPCVLNRIHETPDAFGGNVGCRAMFGGDQRTKCA
                     GKGLMPSEGSDGIGVGESE"
     gene            complement(208..765)
                     /locus_tag="WGO49_000770"
     CDS             complement(208..765)
                     /locus_tag="WGO49_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0305901.1"
                     /translation="MDALKLLTNRRSSKKLKPPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(841..2475)
                     /locus_tag="WGO49_000771"
     CDS             complement(841..2475)
                     /locus_tag="WGO49_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ0305902.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2549..2878)
                     /locus_tag="WGO49_000772"
     CDS             complement(2549..2878)
                     /locus_tag="WGO49_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305903.1"
                     /translation="MSRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPSVMLF
                     VPDCPVGGAAGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRTPPHSRRFLA
                     LLCSVAL"
     gene            complement(2878..3984)
                     /gene="serC"
                     /locus_tag="WGO49_000773"
     CDS             complement(2878..3984)
                     /gene="serC"
                     /locus_tag="WGO49_000773"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ0305904.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGATVVIIRED
                     LLERCPNDIPDVFNYRSHLNREGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTGDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4349..4780
                     /gene="rimP"
                     /locus_tag="WGO49_000774"
     CDS             4349..4780
                     /gene="rimP"
                     /locus_tag="WGO49_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ0305905.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4808..6310
                     /gene="nusA"
                     /locus_tag="WGO49_000775"
     CDS             4808..6310
                     /gene="nusA"
                     /locus_tag="WGO49_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ0305906.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6322..9210
                     /gene="infB"
                     /locus_tag="WGO49_000776"
     CDS             6322..9210
                     /gene="infB"
                     /locus_tag="WGO49_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ0305907.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKTAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLHIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARSL"
     gene            complement(9422..10816)
                     /locus_tag="WGO49_000777"
     CDS             complement(9422..10816)
                     /locus_tag="WGO49_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ0305908.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLAGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(10890..12230)
                     /locus_tag="WGO49_000778"
     CDS             complement(10890..12230)
                     /locus_tag="WGO49_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ0305909.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            12373..12999
                     /locus_tag="WGO49_000779"
     CDS             12373..12999
                     /locus_tag="WGO49_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ0305910.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYVVMGWMVLAVMKSLTASLPLAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13063..14481)
                     /locus_tag="WGO49_000780"
     CDS             complement(13063..14481)
                     /locus_tag="WGO49_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0305911.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDYIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            14808..15536
                     /locus_tag="WGO49_000781"
     CDS             14808..15536
                     /locus_tag="WGO49_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ0305912.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15595..16083)
                     /locus_tag="WGO49_000782"
     CDS             complement(15595..16083)
                     /locus_tag="WGO49_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ0305913.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAVYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(16841..17305)
                     /locus_tag="WGO49_000783"
     CDS             complement(16841..17305)
                     /locus_tag="WGO49_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ0305914.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         4560 a         4848 c         4369 g         3568 t
ORIGIN      
        1 cttctgattc cccgacaccg atgccgtctg aaccttcaga cggcatgaga cctttgcctg
       61 cgcacttggt gcgctggtcg cctccgaaca tggcgcgaca cccgacattt ccgccgaacg
      121 catcgggcgt ttcatgaatc cggtttaaaa cgcatggaaa aatgccgtct gaaagccttt
      181 cagacggcat tgtgcttgag attccgttta ccaatggctg acaaacgctt ccaaatcggt
      241 attcttgggc ttatgcactt cctctgtcgg cgtgccgacc atcatcagcc cgatgatttt
      301 atccttatcc gcacaaccga aagcctcccg caacaagggg ctgttgaccc acatccccgt
      361 aatccagaca ttgtcgaatc cctgagccgt tgccgccagt tgcagcgcat acgccgcaca
      421 acccgccgtc agcatctgct cccattccgg tttcggctta ggcacatcac gattcggcgc
      481 aaacgttacc ccgatgacca tcggcgccat attgcccact ttttccgcct ttttcatcgc
      541 atcgtcgccg aaattcaatt cggcaaccgt ttgctttaac acatcgcgaa aacgttgcaa
      601 tcctacctcg ccttgaatca cggtaaaacg gaaggggcgc atattgccgt gatcgggaac
      661 ttgggttgcc gcctgaaata tttgttccaa ctccgccgca tcgggggcgg ggggcttcag
      721 ctttttggaa gatcggcggt tcgtcaataa ttttaaagca tccatatcgt tatactccgg
      781 tcatcggtcg ggtgttcgga cacatctgcc gtccgaaggc ttcagacggc atatccagca
      841 tcagcgcgga cggcggcagg ctgccaatat atccatttcc ttccgatagg tttggctatt
      901 ggaaatgtcc atcagcccca ataccgtgct gaaatagtgg tcgtgcgaat attcgttttc
      961 cgccgctttt tgtttgaggc attggaaatc tatgcccccg tgttggcgga aggctttgga
     1021 aaaccacata accatcggga tatgcgtctg cccggaaggc gcgatggcgt aaggcgcggc
     1081 gtgcaggtac atcccgtttt cgcccaaact ttcgccgtgg tcggaaacat aatgcaccac
     1141 gctttccaaa tcgtcgcggt tttcaagttt gcggataacc ttgtcgataa actggtccac
     1201 atacaaaacc gtattgtcgt aagtgttgac cagcgtggcg cgggtgcatt tgttgatttc
     1261 gttggtgtcg caggtcggcg tgaatttgcg ttcggcttcg gtatagcgtt cgtaatacgt
     1321 cggcccgtgg ctgccgatgg tatgcaggat taaaaccgcg tctttatcgt ttttgttgag
     1381 gacttcgtcg aacttagtca gcaggatatt gtcgagacac tcgccgttgc ggcagtattc
     1441 gggcaggttg agcgaggtaa cgtcggtatt cggcactttg ccgcacacgc ccttgcagcc
     1501 ggaatcgttt tccaaccagg taacttccac gccggcgcgc tgcacgatgt ccagcaggtt
     1561 gtcttggtgt tcggctttga tttcgtcata atccgtgcgg tcgaaggttg agaacataca
     1621 cggcagggag tgcgcggtcg atgtgccgca gcttctgacc tgcgggaaat tgacaatttc
     1681 atcgccgcgc gcggcaagca gcggcgtagt ttggcggctg taaccgttca aaccccagtt
     1741 ggcggcacgc gtggtctcgc ccacgaccag caccacgaaa cggcgcaggc tgccggccgg
     1801 ccggttttgc acgaccgcca tatccaattg cgtataagga atattggaac gcttccaatc
     1861 tttgtatttc gacacgcccg cgccgatgaa attagatggc acaatcagat gggttactga
     1921 tttattgttg cggaaaaacg aggcgtaatc ctgatattgc aacattgcga tgcccaacgc
     1981 gcacaaaaag gaaacggcgg caagcacaag gcgcgtcaaa agctccttat accaaacgcg
     2041 gtatttaacc ttgacggcga tatacgccag cgcgggcaat acgcccaaac atacaatcca
     2101 cagcacatag cccggcgtaa tcaggcgcgc gctttcggca gccgtagttt gcaagacatt
     2161 attcaacatc gacttgttga aatagatatt gaaaaatatt tcttggtaag acaccgccgc
     2221 actgataacc aatatcaacg gaatcaatac cttatgcacg aaaggcaggg caatgacgtg
     2281 aaaaacgaaa ttacttaaaa aaaacagcac caccggcatc gtatagagga agatatccgc
     2341 cccggtgccg ttaaaaggat gaagctcgac aactttggca aaaaaggcgt aattcaatac
     2401 cagcgaggaa tacagggaaa ggaaggcaat cagcgcggaa gagccgagct tcggcctcag
     2461 gttcggtttt atcatttgga atgtgtcgga taagggttgg aaaaggcatc cggcatttgg
     2521 aatccggatt attgaaaaag attcttaatt ataaggcaac ggagcaaagc agggcaagaa
     2581 aacggcggct gtgcgggggg gtccgcccgc cattcaaacg tccggcagac ataaaaacat
     2641 cgtaagcaag attcgaaccg gtctgcaacc gcccctgcca gaaaaacggg caaagcattt
     2701 catattggaa aaacccggcc gcgccgccga cgggacagtc cggcacaaac agcatcacgc
     2761 tcggattgaa aaggacggat agccgccggc agcacaatct taccacctcc aaaacgccgc
     2821 cggagaacgc cgaaacagcc gatgccgtct gaagccgctt cagacaacat cgggacatca
     2881 accgtaacgc cgttggaaat cgcgcataaa atccgccaaa gcccgcacgc cttcaagcgg
     2941 catcgcatta taaatgctgg cacgcatacc gccgacggtt ttatagccct taagcaggca
     3001 caagccctgc aattcggctt ccagcacaaa acggcggtca agctcctcat cccccgtttg
     3061 gaacacgaca ttcattttag aacgcgcatt cggacggata cggttgatat agaaaccgcc
     3121 gctgccgtct atcgtctcat acaaggtttg cgccttcagc cgattgaccg cttcaatttt
     3181 tttcacaccg ccctgcgcct gcagccagcg gaacaccagc cccgacatat aaatcgcgta
     3241 agttgacggc gtgttgtaca taccttcgcg gttcaaatgc gaacggtagt tgaacacatc
     3301 gggaatatcg ttcggacaac gctcgagcaa atcctcacgg ataatgacca cggtcgcacc
     3361 ggccgggccg atgtttttct gtgcgcctgc gtaaatcagt ccgtagtcgg caacatcaaa
     3421 ctcgcgcgac aaaatctcgc tggacatatc gcacaccagc ggcggcatgc cttctgaaag
     3481 gcgcggcact tcacggtatt gcagcccgtt gaccgtttca ttgacggcaa aatggacaaa
     3541 cgccgaatcg ggtgcaacat cccacgtttc cacaggcggc aggtcgagat agtcgaactg
     3601 ctcgccgcca tgcgccgcca aacggatttc cgtatcggtc aaacggctca tctgttcata
     3661 agcgatacgg ctccagttgc ccgttaccac cgcgtcggca gtgcggaaac cgtgtgccag
     3721 attcatggct gccatattaa attgggttgt tgctccaccc tgcagaaaca atatcttata
     3781 gttgtcaggc actttcaaaa gctgcctcaa atcctgttcc gcatgatgca ggatgctcaa
     3841 aaacatttcc gaacggtggc tcatcgccat cacaggaaaa cccgtaccgt tgtagtccaa
     3901 catttcctgc cgcgccgttt ccaacacggc ttcgggcaat acggcagggc cggcggaaaa
     3961 attgtaaatc ggataaagag acataatgca gccttgattc tgaacaataa cccgcccgat
     4021 tttaggcttg cagcaggctt tgtgcaagga tgggaaatac ctgtcctccg ccccgcttca
     4081 tgccgcccga acacggaaaa caacatcaat atattgattt acaaatataa aaatcatgct
     4141 agcgacaaat agatacattt gttttgtcaa caatattcac gatttcccat tacaaacctc
     4201 ccttacaccc gcttttttcc gtcccaaaaa cacaaaataa atcaacactt tcatttctcc
     4261 gcaaaagcgg ttataatcac gccgattttt caactttgac gaaaaatggc cacggggcca
     4321 tttttttatt atgtacatag ggagcagcat ggatatccaa accatcctcg aaaaaaccct
     4381 gcccggcctg ggctacgaac tggtcgattt cgaactgacc gcgcaaggaa cattgcgcgt
     4441 gttcatcgac aaagaaagcg gcattaccgt cgaagactgc gcaaccgtca gcaaccactt
     4501 gagccgcgtc ttcatggttg aagacatcga ctacaaaaac ctggaaattt ccagccccgg
     4561 actcgaccgc cccttgaaaa aagccgccga cttcgtgcgc tttgccggtc agaatgccaa
     4621 aatcaaaacc cgcctgccga tagacggtca gaaaaacttt atcggtaaaa tcgaaggctg
     4681 cgaaaacgat accgttaccg tatccttcga cggcaaaacc gtacaaatcg aattgggcaa
     4741 catcgacaaa gcccgtctgc gccccgaatt caaattctaa aacacaacaa tattggagat
     4801 gttcaaaatg agtcgtgaaa tgttacagct ggcagaagca ctggcaagcg aaaaaaacgt
     4861 tgatgcggaa gtcgtcttcc aagcactgga attcgccctg tctaccgccg ccaagaaaaa
     4921 ggcagaccgc gaacacatgg acgtgcgcgt ccaaatcaac cgcgacaccg gcgaatacca
     4981 aaccttccgc cgctggctga ttgtcgccga tgaagactat acctatcccg atgtcgaaaa
     5041 aaccatcgag gaaatccaag aggaaattcc cggcactacc atccaaatcg gcgaatacta
     5101 cgaagagcag ctgcccaacg aaggcttcgg ccgccaagcc gcgcaaaccg ccaaacaaat
     5161 catcctgcaa cgcatccgcg atgccgagcg cgagcagaat ctgaacgagt ttctcgccgt
     5221 caaagaagac atcgtgtccg gcacggtcaa acgcgtcgaa cgccacggca tcatcgtcga
     5281 agtcgttgcc ggcaaactgg acgcgctgat tccgcgcgac caaatgattc cgcgcgaaaa
     5341 cttccgcagc ggcgaccgca tccgcgccct cttcctgcgc gtcgaagaaa tcggcaacac
     5401 cggccgcaaa caagtcatcc tgagccgtac ttccggcgat ttcctcgtca aactgtacgc
     5461 caatgaagta cctgaaattg cagacggcat gcttgaaatc cgcgctgtcg cccgcgaccc
     5521 gggccagcgt gccaaagtcg ccgtcaaagc caacgaccag cgcatcgatc cgcaaggcac
     5581 ctgtatcggc gttcgcggtt cgcgtgtcaa tgccgtcagc aacgaattgt ccggcgagcg
     5641 catcgacgtt gtcctttggt cgcccgagcc tgcccaattc gtcatgagcg cgctctcacc
     5701 cgccgaagtc agccgcatcg tcatcgacga agacaaacac gccgtcgatg tcatcgttgc
     5761 cgaagaccag ctcgcgctcg ccatcggacg cggcggtcaa aacgtgcgcc ttgcttccga
     5821 cctgactggc tggcagctca acatcatgac ttccgccgag gcagacgaac gcaatgcggc
     5881 agaagatgcc gccatccgcc gcctgtttat ggatcacttg aacgtggacg aagaaaccgc
     5941 cgacgtactg gttcaggaag gttttgcaac cttggaagaa gtcgcctatg ttcctgccgc
     6001 cgaactgctt gccattgaag gatttgacga agaaatcgtc gatatgctcc gcaaccgcgc
     6061 ccgcgatgcc atcctgacca tggcgattgc cgccgaagaa aaactgggcg aagtgtccga
     6121 cgatatgcgc aacctcgaag gcatagatgc tgatatgctc cgcagccttg ccgaagcagg
     6181 cattactacc cgcgacgact tggcagagct tgccgtggac gaactgattg aaatcaccgg
     6241 tgtaaacgaa gaaaccgcaa aagccgtcat cctgaccgca cgcgaacact ggtttaccga
     6301 agacaaataa agggggtaca gatgagtaac acaaccgtag aacaatttgc cgccgagctg
     6361 aaacgccccg tcgaagacct gttgaaacag ttgaaagaag ccggcgtcag caaaaacagc
     6421 ggcagcgatt ccctgacgct ggacgacaaa cagcttctga acgcctacct gaccaagaaa
     6481 aacggcagca acggcggcac catcagcatc cgccgcacca aaaccgaagt cagcaccgtt
     6541 gacggcgtaa aagtcgaaac acgcaaacgc ggacgcactg tcaacattcc ttctgccgaa
     6601 gagttggcag cacaggtaaa agccgcccaa acccaagccg cacctgtccg gccggagcag
     6661 acggcagaag acgcggcaaa agcccgagcc gaagctgccg cacgtgcaga agcccgtgcc
     6721 aaggcagaag cggaagcggc aaaactgaaa gcggcaaaag caggcaacaa agccaaacct
     6781 gccgcgcaga aacccaccga agcaaaagcc gaaaccgcac ccgttgcggc ggaaaccaaa
     6841 cccgccgaag aaagcaaagc ggaaaaagcc caagccgaca aaatgccgtc taaaaaaccc
     6901 gccgagccca aagaaaaagc cgccaagccg aaacacgagc gaaacggcaa aggcaaagat
     6961 gccaaaaaac cggcaaaacc tgccgcacct gccgtgccgc aacccgtggt cagcgcggaa
     7021 gaacaggcgc aacgcgatga agaagcacgc cgcgccgccg cacttcgcgc ccaccaggaa
     7081 gccctgttga aagagaaaca ggaacgccag gcacgccgcg aagccatgaa acaacaggca
     7141 gaacaacagg caaaaaccgc acaggaagcc aaaaccggca gacagcgtcc cgccaaacct
     7201 gccgagaaac cgcaggcagc cgcgccggcc gtcgaaaaca agcctgtcaa tccggcaaaa
     7261 gcgaaaaaag aagaccgccg caaccgcgat gacgaaggtc aaggccgcaa cgccaaaggc
     7321 aaaggcggaa aaggcgggcg cgaccgcaac aatgcacgca acggcgacga cgagcgcgta
     7381 cgcggcagca aaaaaggcaa aaaactcaaa ctcgagccga accaacacgc cttccaagca
     7441 ccgaccgaac ccgtcgtaca cgaagttttg gtgcctgaaa ccattaccgt tgccgacttg
     7501 gcacacaaaa tggcagtcaa aggcgtggaa gtggtcaaag ccctgatgaa gatgggcatg
     7561 atggttacca tcaaccaatc catcgaccaa gacaccgccc tgattgtcgt cgaagaactc
     7621 ggacacatcg gcaaacccgc cgccgccgac gatcccgaag ccttcttgga cgagggcgcg
     7681 gaagcggtgg aagccgaagc attgccgcgt ccgcccgtcg ttaccgtcat gggccacgtc
     7741 gaccacggca aaacctcgct gctggactac atccgccgta ccaaagtggt acagggcgaa
     7801 gcaggcggca ttacccaaca catcggtgca taccacgttg aaaccccacg cggcgtgatt
     7861 accttcttgg atactccggg tcacgaagcg tttaccgcca tgcgtgcgcg cggtgcgaaa
     7921 gcaaccgata tcgtgattct tgtggtcgcc gccgacgacg gtgtgatgcc gcaaaccatc
     7981 gaagcgattg cccacgccaa agcggcaggc gtaccgatgg tggttgccgt caacaaaatc
     8041 gataaagaag ccgccaaccc agagcgtatc cgccaagagc tgaccgcaca cgaagtcgtg
     8101 cctgacgaat ggggcggcga tgtacagttt atcgacgttt ccgctaaaaa aggcctgcac
     8161 atcgatgcat tgctcgaggc agtcttgctc gaagccgaag ttttggaact gaccgcacct
     8221 gtcgatgcac ctgccaaagg tatcatcgtt gaggcgcgct tggacaaagg ccgcggcgcg
     8281 gttgccacat tgctggttca aagcggcacg ctgaaaaaag gcgatatgct gctggccggt
     8341 acggcattcg gcaaaatccg cgcgatggtc gatgaaaacg gcaaatccat taccgaagcc
     8401 ggtccgtcca tccccgtcga aatcctcggc ttgtccgacg taccgaatgc gggtgaagac
     8461 gcgatggtat tggcggacga gaaaaaagcg cgcgaaatcg ccctcttccg ccaaggcaaa
     8521 taccgcgacg tgcgccttgc caaacagcag gcggcgaagc tggaaaatat gttcaacaat
     8581 atgggcgaaa cccaggccca atccttgtcc gtcatcatca aagcagacgt gcagggctct
     8641 tacgaggctt tggcgggcag tctgaaaaaa ctgtccacag acgaagtgaa agtgaacgtg
     8701 ttgcacagcg gcgtgggcgg cattaccgaa tcggatgtca accttgccat cgcgtccggg
     8761 gcgttcatta tcggctttaa cgtgcgtgca gatgcctctt cgcgcaaact tgccgaaaat
     8821 gaaaatgtgg aaatccgcta ctacaacatc atctatgatg ccatcgacga cgtgaaagcc
     8881 gccatgagcg gtatgctttc cccggaagag aaagaacagg ttaccggtac ggtcgaaatc
     8941 cgtcaggtca tctccgtttc caaagtcggc aacattgcag gctgtatggt taccgatggt
     9001 gtggtcaaac gcgattccca tgtccgcctc atccgcaaca acgtggtcat ccacacgggc
     9061 gaactggctt cgttgaaacg ctataaagac gacgtaaaag aagtccgcat gggctttgag
     9121 tgcggtctga tgctcaaagg ctacaacgaa atcatggaag gcgaccaact ggaatgcttc
     9181 gacatcgtcg aagttgcccg cagcctgtaa ttcctttgca aataaaatgc cgtctgaagc
     9241 gttcagacgg catacgaaac gggttctgta tcatacagaa cccgtttttt gtcgcaaatc
     9301 ggcttcagac agccctcttg ccttatcccg atttgaatct gacttgccat acaaacaggc
     9361 ttcagacggc attatagtga attaaattta aaccggtaca gcgttggctc gccttgccgt
     9421 actatttgta ctgtctgcgg ctcgccgcct tgtcctgatt taaatttaat tcactatatt
     9481 ttcccgacag gcggatttga agtttctcca catatccggc gcgttcggcg cggctggttt
     9541 cggggcggtg cgtattgagc gacgaccatt tccaatgact gcgggctttg ttgagttcgg
     9601 gcgggagtct ggcggcgtcc cacggggctt tgcggctgtg caactcgata tccgattgtg
     9661 ccgcgtgtcc gcgcgtttgc aggacgtgga gcagatcgag ggcgcgggcg gcgagcaggg
     9721 tcagggtttc agggtcggtg tgcaaggttt ggcggccggc gagtttgtcg gaaatggtgc
     9781 gggtattggg caggatgccg cccaaaaagc cgccgattgc cgtacccaat ccgagcgagc
     9841 cgccgagtgt ggcgatgtcc agccccaagc cgatgagtgc gccggttgcc gcgcccgtgc
     9901 cggtgcggat gccgtattgt ttgagcaatt cgctgtcgaa cgggtcttgg cggaaggctt
     9961 gcggcatcca gtcgccgccg tcgatttcgc tgtggtagaa acggtagagg gcaaacagcc
    10021 gctgctgcat ctgccgttcg agttggcgta tttccgcctg catggtttgt agcacggtgg
    10081 cggtatcctc gttttcgtcc acttcctgcc tgaaggcggc ggcatcgagc aggaaatcgg
    10141 cgatttcgcg gcgcgcttcg ccgtccagcc gctgccattc gcgccggcgc atggctgtca
    10201 ggcggtcaag tgtgctgcgt tcgggcaaca tggtggcgag gttttcccac aggcgtagtt
    10261 cgccttcaaa atcaaaggcg acggtgtcga accctgcgaa aacgtgcagg tttctcctcg
    10321 ccagcatggt tgtccacgat tcgggaagct gtccgccggt aaagttgaac acgggcataa
    10381 ctggtttggc acaccatgaa aggatggtca attcatcccg atatttgtcg aggacgggct
    10441 cgcgcgcgtc gatgacgtac attgccatat cgctttgcaa gacttgccgt aagactttgg
    10501 cttcctgatt gaaatcatgg tgcgcgccgt ggctgccgag aaactgttgc agccgttcga
    10561 tgccgtctga acgattgtcc gtatggtttt ccagccattc cagcacgccg cccgcgtctt
    10621 caagtccggg cgtgtcgtac aggaaaacca gcgtgtctgc gccgtcgctg atggcggctt
    10681 cttcgacatg gcgtgtggtc gatggggcgt ttctgacttc gccgaaaccg ctgtcgcgca
    10741 acagggtacg aaggagcgaa gttttgccgg tattggtatg cccgacgacg gcgagggaaa
    10801 ggggttgttt gttcatgatg tttttgaaga atggattttc agacggtctt ttttcagaat
    10861 ggcggcttaa cagaacattt caagtgagtt tattggtctt tcaaacgccc ttcctgcgcc
    10921 gccctgtcag gctcaagcca cgccgcgccg cattcggcca gcgcgttacg ccaatgttcc
    10981 agcttttccg aaaggtcgtc tgaaagcccc tgttccgcca aaagctgcac caccgcgccg
    11041 ccctgcgccg cttccgaaag tcggacgatc tgccgcaaca cgccgcggtc gggcacagtt
    11101 tgggcgcgca cgccgataag cagttgcgcc ggtttctgct tcagctctgt ctccagcgcg
    11161 gcaacctgtt cccgattggc ggcaacgccc ttatccagcc attcctgcgc cagcctgccc
    11221 tcgaaccatt cgccgtcctg ccattcggtc tccagcatga ccgcccattt cggcgcatcg
    11281 ttcaagacga ttttcggcga aacggcggac acggtttccc gacgcgtatc cgcatcggtg
    11341 attttgttct gccagcggcg gatgaccgcc tgataatagg gtttttccaa atccaatccg
    11401 ttttcgcttg ttttcaaaag gattttgcat accgcccaag ccaagaggcg cggcaggatg
    11461 ccgtagcagg cgatactgcc gaccagcagc cccgaccaag cccgcgcatc ggcaatattg
    11521 ccgttcagac ggccttcgat gaccgcccgc gcatcaggca cgggaaaacc cagtttcgca
    11581 ggcagccatg ccaacatttc caccagccgt accgaagacg aatcgcccaa cagcgtgctt
    11641 tcccagttga acgtatattg ccgcaccaaa agcagcaaca ataccgacac cagcattccg
    11701 agcagcgtgc agagccacag gctgtgcgac gttgcgccta ttttccaacg taccgaaggt
    11761 tgccgccact cgtccgcata cagccgcaac accgcctgat ttacagggtc tttgccccga
    11821 aaccacgtcg ccggactgct gaaaaaacgc cccactttca cgcgcaggaa caacattgcc
    11881 aaccatactg ccagcatcag cgtattcatg cccaacacgc ccgccaaaac caaaaagaaa
    11941 ttcagaccct gattgtccat tagaagataa gtgcctgaaa aaccggtaaa aaacgcaaac
    12001 gtcgccgccg ccacccacaa ccagaacgac cccgcacgca cacgttccaa tgtgtcccgc
    12061 agcatacggt tcctgtcgat catctccgcc cgacggatga ttttttcctc cgtactgccg
    12121 tccacgcggc gcaaagcctc cgtcgcctgc acgggatcgc cgctgaaaat aaaaccgcct
    12181 tcgtccaaaa tacggatcag ctcaaccagt tttcgggatg gattcaacat aaaatgccgt
    12241 ctgaaaataa aaaacagatt ttaacacacg cattttcaag aatattcaca gtgtaggcaa
    12301 agagtaaatc tcacacagaa gcaaaagtat cggcgtaaac tgactgcctc tactttcccg
    12361 aaagattgtg cgatgtatac aggcgaacgc ttcaatactt acagccattt gagcggtttg
    12421 attctggcgg cggcaggttt ggcgctgatg ctgctgaaaa ccataggaca cggggacggc
    12481 taccgtatct tcagcgtatc ggtttacggc atcagccttc ttctgctcta tttgagttcc
    12541 tcgctgtacc acggaattgc agccggaaaa ctgaaaagca ttttgaaaaa aaccgaccac
    12601 tgcatgattt atgtgctgat tgccggaagc tacacaccgt ttgcactggt ttctttgaga
    12661 aacgggccgg gctggacggt attttcactg tcctggctgc tggcggctgc aggaatcgca
    12721 caagaactca ccatcggacg gaaaagcgaa aaacgactgc tgtctattgc gatttatgtc
    12781 gtcatgggtt ggatggtctt ggcggtaatg aaatccctga cagcctcact cccgctggca
    12841 ggactggctt ggctggcggc aggcggtatg ctgtacagcg tcggcattta ctggtttgta
    12901 aacgatgaaa aaatccgaca cgggcacgga atctggcatc tgttcgtatt gggcggcagc
    12961 atcacccaat ttgtcagtgt gtacggttat gtaatctgaa tgccgtctga aaagcaaaac
    13021 ctcccgatcc tgaagattgg gaggttttct gtttgccgga catcagccct tgtcgtggaa
    13081 ctcgtgaaat tcatactgat aggacaaatc ccgacccgct tttttctgtg ccaaataatc
    13141 atcataaatg gcacggattt ccttacgcaa caaaaacagg gctatcaagt tcgggataac
    13201 cataaagccg ttgaacatat cggacaggct ccaaaccaaa tcgactttgc cgagcgtacc
    13261 caaaacgatg gcaagcagaa ccaatgcgcg atagatgccc aagtgtcttc ccctgaaaag
    13321 aaaacggatg ttggactcac cgaaataata ccagccgata atggtcgtca aggcgaaaaa
    13381 ggtcagacac acggcaagca attgcgaacc gaagcccgga aatgccttgt taaaggcaaa
    13441 ctgggtaacc gccgcgccct gttcgcccga aaggttggca tcagtcagca ggataatcaa
    13501 tgccgtagcc gtacatacca aaatcgtatc gataaacaca ccgacaaatg ccgccatacc
    13561 ttgctgcaca ggatgcttca catcagcggt tgcatgtgca tgcggggtag aacccatacc
    13621 tgcttcgttg gaaaacagac cgcgcgccac gccgaaacgt atcgcttcac gcataccgat
    13681 acccgcagca ccgcccaaaa cggcttcagg attgaaggcg gcggtaaaga tgtggttgaa
    13741 catcggcaca atatagtcgg aaaattcaaa caggataacg acggcgcaca aaatataaac
    13801 aaccgccata aacggcacga caaactgggc aatattggca atacggttca cgccgccaat
    13861 cacaaccatg cccgcaagga cggcaagcac aataccgact gccaaagaag gcacatcaaa
    13921 tgcaatggta acggcagaag caatggagtt tgcctgtgtc gcattaccga taaagcccaa
    13981 tgcgataatc aacgcaatgg agaaaaagcc cgacaggaaa cgcgccgcgc ccctgccgat
    14041 tttcggagtc agaccgtgcg taatataaaa cgccggccca ccgatgtatt tgccgtggct
    14101 gacgacgcgg tatttctgcg ccagcagtgc ctccgcaaaa atcgtggaca tccccaaaac
    14161 ggcagaaacc cacatccaaa aaatcgcgcc cggcccgcct gcggtgatgg cggtcgccac
    14221 gccggcgacg ttgcccgtac cgatttgcgc ggatatggca accgccaacg cctgaaactg
    14281 cgataaagac ttatcgtctt tatcgccttt ggcaaacaag ccgccgaata cagatttgaa
    14341 tcccgcaccc agcttggtaa tctgcggcgc accgagatac agcgtaaaaa acaggccgat
    14401 acccaaaagc gcgtaaatca gcaggtagtc ccaaaggaac cgattgactg tacccaccag
    14461 aacagacaat atattttcca taaaataaac cttatcttac aattaaaatg actgccttcc
    14521 aaaagacatt ccaataagga aacacggcga gcagaccgta tttgccgcaa cagatgcctt
    14581 aaattgtcaa caatcgggga gaagctgcgc catcataccg taaaatatcg ttaattaaaa
    14641 catttcttta tttttaagcg gaaagcggag gaaatcgctt tcagacggca tcgacaacgg
    14701 cacggcataa aacaggatat tttgggtact tgcaacttat gttaaaatgc cgaccgtaaa
    14761 aaatctgaca aaaacagatt aattatttga aataagaaag gaaatttatg gcaggtcata
    14821 gcaagtgggc gaatatccag cataaaaaag cccgtcagga tgccaaacgc ggcaaaatct
    14881 tcacccgttt aatcaaagaa atcaccgttg cggcgcgtat gggcggcggc gatcccggcg
    14941 caaatccgcg cctgcgcctg gctttggaaa aagcagccga aaacaatatg cccaaagaca
    15001 atgtgcaacg cgccatcgac aaaggcacgg gcaacttgga aggcgtggaa tacatcgaat
    15061 tgcgctacga aggctacggc atcggcggcg cggctttgat ggtggactgc ctgaccgaca
    15121 acaaaacccg caccgttgcg gacgtacgcc acgcgtttac caaaaacggc ggcaacttgg
    15181 gtaccgacgg ctgcgtggcg ttcaacttcg tgcatcaggg ctatttggta ttcgaacccg
    15241 gcgttgacga agacgcgctg atggaagcgg ctttggaagc cggtgcggaa gacgtggtta
    15301 ccaacgacga cggttccatc gaagtcatta ccgcgccaaa cgattgggcg ggcgtaaaat
    15361 ccgctttgga ggcggcaggt tacaaatccg ttgacggcga cgttacgatg cgcgcccaaa
    15421 acgaaaccga actctccggc gacgatgccg tcaaaatgca aaaactgatt gacgcgctgg
    15481 aagacttgga cgacgtgcaa gacgtttaca cttccgccgt attgaatttg gactgatacg
    15541 cagcacagca gacatacaat aaaatgccgt ctgaaccttt cagacggcat tgatttattt
    15601 agcccttgcc cacgcccacc aaaccgtcag gacaaccgcc agaaaatacg ccgcgctcca
    15661 aacaggcaaa gcaaccccta acagataatc cggttcggca caatttccga acccgcgcac
    15721 gacaggctcg aaccaatcaa acaaagacca gcccttcaag cggaacgtcc acggcgcacc
    15781 gcacgaagga gcggttcccg gcggcagcga ctgcaaccac aactgatata ccgcaacaga
    15841 aatacccgta acggccggaa tgctgataaa gacagcaccg aacaaaccgc ccgcccttct
    15901 tcttggtctg cacatcagga caattgccgt acacaatgcg gttgccaaaa cgcataaccg
    15961 ctgactgata cacaaaacgc aaggctccat acccaaaaca tactgtgccg ccaaagaacc
    16021 ggcaaatgca cagaccgaaa cggcaaacag cagccaaacg gcttttctaa ataacggggt
    16081 cattttctca acacaccaat caaaataccg atatgccgat tttgctggat atatcccgag
    16141 atggcaaggg acgaatcgac accggcgcag gtgcggacga tttcggtttt cccgatatac
    16201 acaagacctg acaagggaca aaacggcgat ttgcccgcac aatgcccgca aaaaaataca
    16261 tccggaggat ttgaatttcg gcaaatttag cgaatcaaaa gtttatttca atgaaattat
    16321 atgatttttt tgaataagcg gattgacagg tttttgaagg aatttgttac cggatagcca
    16381 tcgggcaagt tttttgcaaa atttgaatcg gtcgggtaaa ttttcaaaaa ataattgaca
    16441 gcggataaga aacggcggat aattcccgcc gtcgagttgc ttgatgcagc ttgatttttc
    16501 tcctctattt ctcctttgta gacttggcac acattcaact ggatgtgtgc atttttttat
    16561 ctccgctttt tttgaaattt aattactttt attgtttgaa attccattct ttaagaaatt
    16621 taacaagaaa aacgcttgct ttttgaccgg aaaagctgca taattcgttc cgttagctgg
    16681 ttcgagtagt cagttaatag tttctcctct atttctcctt tgtagacttg gcacacattc
    16741 tattgaatgt gtgcattttt ttatctgaag caacaagcct ctgtgcgtga tgttgttatg
    16801 tttcatttag gtgtcaaacc gcatacccgg tctgaaatat tcaatccaaa tccaaaaccg
    16861 gattttcttt gacctcctcc atcacaacat aactcctact ctccgaagcg gcaggcagtt
    16921 gcaataggat attgcctagc atatcccgat aggcagacat atcgggcaaa cgtactttaa
    16981 tcagatagtc gtattcgccc gacaccaagt ggcattccat aatttgcgga attttcagca
    17041 cttctttttt gaaatcttcg aaaatattgc ccgatttgga ttgcagcttc agctcgacaa
    17101 aaaccaatag cggtttgccc aacagatggg gattgaggtg ggcgtgatag ccggaaatat
    17161 aatgttcccg ctccaaacgg cgcaccctct ctgtaacggg cgtggtggac aagcctacct
    17221 tctcggcaag ctccgtcatc gggatgcggg cattctgttg gaggatctta agaatgcgga
    17281 aatcgatttt atctagttct ttcatttgga ttgccttgta tttattattg attttaacaa
    17341 ataga
//
LOCUS       DAWFAD010000032        17088 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWFAD010000032 DAWFAD010000000
VERSION     DAWFAD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.032"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(117..1286)
                     /locus_tag="WGO49_000784"
     CDS             complement(117..1286)
                     /locus_tag="WGO49_000784"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ0305915.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1469..1579)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1632..2528
                     /locus_tag="WGO49_000785"
     CDS             1632..2528
                     /locus_tag="WGO49_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ0305916.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2572..3636)
                     /gene="tsaD"
                     /locus_tag="WGO49_000786"
     CDS             complement(2572..3636)
                     /gene="tsaD"
                     /locus_tag="WGO49_000786"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ0305917.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALAFAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLKT
                     GREAGAFNVRPRWPLSEIVK"
     gene            complement(3752..4939)
                     /gene="ccsB"
                     /locus_tag="WGO49_000787"
     CDS             complement(3752..4939)
                     /gene="ccsB"
                     /locus_tag="WGO49_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ0305918.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEEKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4932..6884)
                     /locus_tag="WGO49_000788"
     CDS             complement(4932..6884)
                     /locus_tag="WGO49_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ0305919.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTVSREGGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7161..7784)
                     /locus_tag="WGO49_000789"
     CDS             complement(7161..7784)
                     /locus_tag="WGO49_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ0305920.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7990..8619
                     /locus_tag="WGO49_000790"
     CDS             7990..8619
                     /locus_tag="WGO49_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ0305921.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8740..11136)
                     /locus_tag="WGO49_000791"
     CDS             complement(8740..11136)
                     /locus_tag="WGO49_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ0305922.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVQKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLEAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11288..12403
                     /locus_tag="WGO49_000792"
     CDS             11288..12403
                     /locus_tag="WGO49_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ0305923.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12406..13005
                     /locus_tag="WGO49_000793"
     CDS             12406..13005
                     /locus_tag="WGO49_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ0305924.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKVEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            13006..13653
                     /locus_tag="WGO49_000794"
     CDS             13006..13653
                     /locus_tag="WGO49_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ0305925.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13671..14216
                     /locus_tag="WGO49_000795"
     CDS             13671..14216
                     /locus_tag="WGO49_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ0305926.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14235..16544
                     /locus_tag="WGO49_000796"
     CDS             14235..16544
                     /locus_tag="WGO49_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ0305927.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQTAAPAKQQ
                     AAAPAKQTNIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRS
                     LDVADFKTPVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESG
                     GVNNAPKTFTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWD
                     QALDLVMQARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKY
                     KNVEEFRSILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDE
                     LDVPAQQVMIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKW
                     GAETKINLPITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAK
                     IESGYEIPFTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQC
                     ASGNQTILCISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFK
                     TRGKKTDRRELLIFITPRIMGTAGNSLRY"
BASE COUNT         4609 a         4855 c         3891 g         3733 t
ORIGIN      
        1 ttccgtacta tctgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
       61 tatataaaaa tgccgtctga actgttcaga cggcattttt gattttcaaa ccggaattac
      121 agccccgctg ccgccctcaa tgcagcagct ttgtcggtgc gctcccaagt gaactcaggt
      181 tcttcgcggc cgaaatgtcc gtaagcggcg gatttactgt aaatcgggcg caagagatcg
      241 agcatttgga cgatgccttt ggggcgcagg tcgaaatgtt cgcgaactaa ggtaatcagt
      301 ttttcctcgc tgattttgcc tgtgccgaaa gtgtcgatgg aaatcgaagt cggttcggca
      361 acgccgatgg cgtaggaaac ttggatttgg cattgggttg ccaaaccggc ggcgacgatg
      421 tttttcgcaa cgtagcggca ggcgtaagcg gcggaacgat ccactttgga cgggtctttg
      481 ccggagaacg cgccgccgcc gtgcggagcc gcgccgccgt aggtatcaac gatgatttta
      541 cggccggtca aaccgcagtc gccttgcggg ccgccgatga cgaagcggcc ggtcgggttg
      601 atcaggtatt tggtttcgtc ggtcagcagt tcagacggca ggacgggctt gataatctgc
      661 tcaatgacgg cgtttttcaa ctcttcataa ccgatttccg ggtcgtgttg cgtagagagg
      721 acgacggtat caatgcgttt tactttgccg gattcgctat cgtaaaccac ggtcagttgg
      781 gcttttgcgt cgggacgcag ccaaggcagg aggccgtctt tgcgtaattc gctttgacgc
      841 tgcatcaggc ggtggctgta atagatggca aacggcatca gggtcggggt ttcgtcgcag
      901 gcatagccga acatcaaacc ttggtcgcca gcgccttggt tcaagtcaat gccttcgcct
      961 tcgttcacgc cttgggcgat gtccggagat tgctggtcgt agtacacgcc gaccgcgcag
     1021 ccgttggcat caaagcccag ctcggaggag ttgtagccga tgcgtttgat ggtttcgcgt
     1081 gcgactttga tgtagtccac ttgggcggtg gtggtgattt cgcctgccaa tacgcacaag
     1141 cctgtgttga ccaaagtttc tgcggcgaca cgcgctttgg ggtcttgcgc caagatggca
     1201 tccaaaatcg cgtcggatac ttggtcggca actttgtccg gatggccttc ggataccgat
     1261 tcggaagtaa acagatattc gctcattgag attttccttg aaaaacaaac catcttcttc
     1321 agacggcatg ttgtatgaac ataatgtcga cagcgggaaa tatagcaaaa tttccctatt
     1381 cataccattc agttgagaaa tattcccatt tgaatggcac tttggaatct ctgcccgtac
     1441 gtttcttaca ggcaaaaaaa ttcccgcatc aagcgggttt ggattgctct ggtgagccac
     1501 atcggctttt caaccgtcca ccttactttt ccttttgaaa agcaggttgg catggaattc
     1561 ccaactctta atgcagcgcg gatggtagca gaaaagcctt tccgccgcaa tacttccctt
     1621 tttcagacgg catgtttcgt ttacaattcg ggctgtttcc ccctttgcga accgccatgc
     1681 acatcctgtt gaccgccctg ctcaaatgcc tctccctgct gccactttcc tgtctgcaca
     1741 cgctgggaaa ccggctcgga catctggcgt tttacctttt aaaggaagac cgcgcgcgca
     1801 tcgtcgccaa tatgcgtcag gcaggcatga atcccgaccc caaaacagtc aaagccgttt
     1861 ttgcggaaac ggcaaaaggc ggtttggaac ttgcccccgc gtttttcaga aaaccggaag
     1921 acatagaaac aatgttcaaa gcggtacacg gctgggaaca tgtgcagcag gctttggaca
     1981 aacacgaagg gctgctattc atcacgccgc acatcggcag ctacgatttg ggcggacgct
     2041 acatcagcca gcagcttccg ttcccgctga ccgccatgta caaaccgccg aaaatcaaag
     2101 cgatagacaa aatcatgcag gcgggcaggg ttcgcggcaa aggaaaaacc gcgcctacca
     2161 gcatacaagg ggtcaaacaa atcatcaaag ccctgcgttc gggcgaagca accatcgtcc
     2221 tgcccgacca cgtcccctcc cctcaagaag gcggggaagg cgtatgggtg gatttcttcg
     2281 gcaaacctgc ctataccatg acgctggcgg caaaattggc acacgtcaaa ggcgtgaaaa
     2341 ccctgttttt ctgctgcgaa cgcctgcctg gcggacaagg tttcgatttg cacatccgcc
     2401 ccgtccaagg ggaattgaac ggcgacaaag cccacgatgc cgccgtgttc aaccgcaatg
     2461 ccgaatattg gatacgccgt tttccgacgc agtatctgtt tatgtacaac cgctacaaaa
     2521 tgccgtaacg aaaataaaaa tgccgtctga acaatttcag acggcatttt gtcatttgac
     2581 gatttccgac agcggccagc gcgggcgtac attaaatgca ccggcttccc tacccgtttt
     2641 caaatgcatc gcgcctgcaa aggcaatcat ggcaccgttg tccgtgcagt atgccgtcgg
     2701 cgggaaaaac acgctgactt tttcggacgg atgtttcggc ttgcctttgg gggtcgggat
     2761 ttgcaccgtc atgttgccga aagtttcacg gagtttgcgg tttgcaccga ccccgccggc
     2821 gaccactacg gttctgaacc ctgtctgcaa cagggctttt ttcactttcg ccgccaacac
     2881 atcgactacc gcatcttgaa acgcacggca gatgtcgttg cgtgtctgct caggaatgtc
     2941 atccgccccg ttttccgcgc gcactttctc gacggcagtc aaaacagcgg ttttcaaacc
     3001 tgaaaaactc atctgcaaat cgtcggaatg aatcatcggg cgcggaaaaa caaacgcttc
     3061 gggcctgccc gattccgcaa gttccgacag tttcgcaccg cccggatacg gcaagcccag
     3121 cagtttcgcc gttttgtcga atgcctcgcc cgccgcatca tcgacgctct cgcccaaaag
     3181 cgcgtagtcg cctatgcccc tgaccgccat aatctgcgta tgcccgcccg aaaccaacag
     3241 cgcgacaaaa ggaaagtcgg gtttttcctc cgccaacagc ggcgacagca gatgcccttc
     3301 caaatgatgg acgggaataa caggcttatc cagcgcaaaa gccaaggcat tggcgtaact
     3361 cgaacccgcc agcaacgcgc cgcccaaacc gggcccctgc gtaaaggcaa ccgcgtcaat
     3421 gtcgccatac gatgcgcctg cctgcgccag acagccttcc gtcaacggaa caaggcggcg
     3481 gatatggtcg cggcttgcca gctccggcac aaccccgccg tattcggcgt gcattgccat
     3541 ctgcgtgtgc agacagtgcg cccgcaatcc tcgttcggtg tcatacagcg caacgcctgt
     3601 ttcgtcgcaa gaagattcga ttcccaatac caacatggtc tgatgccgtt aaaaactgaa
     3661 aaacgtattc tagcggattt cggcacgact gccgtatccc aaaaacggaa catgccgtct
     3721 gaagcccttc agacggcatc gtcgcaccgt atcaaagcgt tccgtaagaa tgcagcccgc
     3781 tcaaaaacat attcacgccg ataaaggcaa atgcggttac gaacaaaccg ataatcgccc
     3841 accacgccag cactttgccg cgccaaccgg caaccagccg caagtgcagc caaacggcgt
     3901 aattgagcca gacgatgaac gcccacgtct ctttcgggtc ccaactccaa tagcgtcccc
     3961 aagcatccgc cgcccacagc gcacccaaaa tggtggcaat ggtaaagaac agaaagccga
     4021 cggcaatcgc cttatacata acttcctcaa tcaacacgga ttgcggcagc cacgatttcc
     4081 cgcctttttc ttccgcacgc agggaaacca gttcggcaat accgagcatc gcggaaatgc
     4141 aaaacgcgcc gtaaccgata aagtttgccg gaacgtggat tttcatccac caagattgca
     4201 atgccggaat cagcggctgg atggtatgcg cctcgcggga cacgctgtac cacaagacaa
     4261 atccaaccac gaccgccata aagccgaaca cgaagccgcc caatttctgt atggcgaact
     4321 taccttcata ataaagatac atcagcgcgg taatcaccaa aaacaggatg aacacttcat
     4381 acaggttgga aaccggaata tgccccgcat cgggacggag cagatagctt tcgtgccaac
     4441 gtaccagcag accggtaaag cctgctacgg cagacaccca tgcaaacacg gttcccatac
     4501 ccaacagcgt gttggtcggc acatttttta cgcttgccaa aaccgcgccc gaaatatagg
     4561 cgaacagggc gaagaagaca aaggcgcact gccacataat cgccgactgg ctgctgagga
     4621 aataccgcaa caggaaaatc tctgccgatt tgatgttgcc gccatacaaa ccgacggcgg
     4681 cataggcaag caatacgctt aaaggaacaa accagcgcat cggtttgaaa aaccaaccca
     4741 aaaacacggc aataccggca cttgcccaca acatgaccgt ttcgtaaatg tccatatgca
     4801 taccggaacg ggtctgtacg aaaaccgtag ccgcaaaaac cagcacggca aatacccaat
     4861 cccaaagatt cagattgctg atcaaagact tctgaatcag cagctcgtgt tccggaaggg
     4921 ttttatagtg ttcagtcatg attcaagtcc ttgccgagcc gttgcagact ctcgacgtgt
     4981 tttggaaatt ccttctgcaa atcccgttcg ctgcgggccg aagacatggc aaaacggatt
     5041 ttgccgtctg aaaacaatac ccacgcccgt ttttcgcgca cataaaacat caataccgta
     5101 cccaatacca acagcaccga gccgagatag accaaaagcg cacccgggga acgggtcatc
     5161 tgcaaacccg acgaacgcac ctcggaaaac ccatcaagtt gcagcagcat aggcgcggga
     5221 tattcggtca aacccgtgta cgcatccata ctgtgcagca ggaaacggtt ccgcgcttca
     5281 tcctgctgcc attcgggcaa gccgtaccgg cgtatggttt catccaaagc agcgttcatc
     5341 acgccgtaaa gcatttcgta gaaataaccc tgcatcttat cctgctgctc tttcgggata
     5401 ttggacgtaa taaattcgtc caatcccaaa tagccttttt gtgcaaagat gttcagcgtg
     5461 ttttccgcag ccagcatgaa ttgttcgcgg atttcggcag gtgcgtcttt ggttgcgtcg
     5521 gcaaccagac gtttgcgccc ttccccatct ttcaaaaact cacgcaatgc cataaaggtg
     5581 tccgctttca actgcttgtc caaggggata cgcagccagc ggtattgctg ctgcaagccg
     5641 ctgcgcgtgc cggtaatcca aaaataatcc tgttcctgca aaaccggcag catatagttt
     5701 ttatattcga ccgcctgccc tgccgcatca cggatacggt aaacaatgga agggccgata
     5761 ttggtgtatt ttttaccttc ctgagtaacg gcgcggacat cgttcagcgt ggatttcagg
     5821 cttttttccc gttccgcgcc ctcgctcatg tcctccacat tcatagaagt gaactgatcg
     5881 aactcaagac gatatttgtg tttgccaatt tccaacggaa actggtgtat ggatgttgcc
     5941 ttcaacacga caggctcgcg cgaagcatca cccaaattcc acgccttgaa tgtcaaatcc
     6001 gaaccgccgt cggcaaaact cgcctgataa atcgtgatgc cgtgcaaggt caaaggatgg
     6061 ttcacgcgga tggtgcgctc gagtttctca ccggttgcct tgtccgtcac ttcaatatcg
     6121 ctggcgaaat cacgcggcat acccgtattg taaaaatcga tatggaattt tttcagtttg
     6181 acttcaaaag gcaagtcctg aaccaatatc ccgttgtcgg cattcaggaa aaccacatcc
     6241 gcactctgcc cctcggaaat attgacgttg cccctaaatg agagattgga cgcactcaaa
     6301 atactttcgg gcttgaaatc cttggcataa accgcctgat tgtccggaac aatccgaccg
     6361 gtcagcatac ccagtttcaa cagcaggtta ctgtctatca acccgcccag gcaaatgaca
     6421 atcaaagcaa catgggcaaa gatatagccc catttgttca tcgtgccttt tttggcggca
     6481 atcagaaccg acccgccctc acggctgacg gtttttccct gaaaaccctg cacctccaga
     6541 taacgtttgg caacttcggg ggcaattttt acatccaaca gcgaagaatg gcgcatcgcc
     6601 gccagagatt tttctttaac cttttcccga aaagacttca tttcgcgcca gaacggcggc
     6661 acattgcgaa tcaggcacaa actggtagaa accaccaaaa acatcatgat aacgacaaac
     6721 catgccgaag catagacgtc atacagtccc agaaaaccaa aaatctgcgc ccaaaaaggt
     6781 ccgaatttga ccaaataatc cgtctgcggc tggttttgct gcaacaccgt accgataacc
     6841 gatgcaatac ccagcagact gagcaaagcg accgcaaagc gcatggagct gaaaaaagcg
     6901 aaccacggac gggaaagaag tgggggagat ctacgggatt tactcattgt gtgtttattc
     6961 cgccatcagg aatatgggaa agcagaattg ggcaaacaga aaacaacgtc ccgattctac
     7021 tgtcttgatg ctttttattt caagacaatg aagacagcct gcatcgattc caacggttgc
     7081 gattgaaaaa acttatcgca gaattgcctg aagccgtctg aaaacttttc agacggcctc
     7141 taaaacagac tattgcggaa ttaacgcaaa ccttggataa agttggcgac cgctttcaaa
     7201 tcttcttcag acatacggtt tgcaatatct tccatgatgg tatttttacg ctgaccggac
     7261 ttgtaggcat tcatctgttc aacaatatat gcctgatgct gaccgcccaa acgcggataa
     7321 gcctgaattt cgcttccgcc tcccggcata cccgcaccgc tcggaccgtg gcaggacata
     7381 cacgccggca cttttttatc gctcaaaccg ccgcgataga ttttcgcacc caattcggga
     7441 ttttccttag gattggcttc accggatttg ggctgctgtt tggcatagaa tgcggatacg
     7501 ttcaaaatat cctgatcgct caaattcatt accaccggtt tcatcacagc tgccgaaccg
     7561 tgggtgcgtt taccgtcgcg gatgccgata gtttgatgat agatgtaagc agtatgctgt
     7621 gccgccaaac gcggatacat cgcaatgccg ctgttaccgt ctgctgcatg gcaagccgca
     7681 caaaccgttg cggcaacctg tttgcctttt tccacgtctg ctttgggaga ggcggaaacc
     7741 gcaccggcag ccaaaacaaa ggccaataaa gtcaatcgtt tcatggagtg ctcctgatta
     7801 cagcattgga taacgcaaca atgctctttt tatattcaaa tacgggattt ttgacccgat
     7861 taaaaccgat gattctgtaa acgtgttatt ctatactaaa tttacattaa attaccactg
     7921 tgtttcacat aaaaccaacc gcatattttt gctgtcggac aaacggcggc ggaaaacaag
     7981 gatatgccca tgaacctttt tcaaaacgcc aaattcttca cgacggtcaa ccaccttaaa
     8041 gacctgcccg acacccctct cgaaattgcc tttgtcggca ggagcaatgc cggaaaatcc
     8101 agtgccatca ataccctgac caaccatgtc cgtcttgcct acgtttcaaa aacacccgga
     8161 cggacgcagc atatcaactt cttcgagctg cagaacggca attttatggt cgatttgccc
     8221 ggctacggtt atgcccaagt ccccgaagca gtacgcgcac attgggtcaa tctgctcggc
     8281 gactatctgc aacagcgcaa acagcttatc gggctggttt tgattatgga tgcccgccat
     8341 cctttaaaag aactcgacat ccgtatgctg gactttttcc acacgaccgg cagaccggtt
     8401 cacatcctgc tgtcaaaagc cgacaaatta tccaaaaacg aacagataaa aaccctgtcc
     8461 caagtcaaaa aactgctcaa accttattcc gacaggcaaa acatcagcgt acagctgttt
     8521 tccagcctga aaaaacaagg tattgacgag gccaaccgaa ctgtcggaag ctggtttgac
     8581 gcagcagatg ccgccgcttc ctctccaaag gaaaactgac cccaattata cggaaaccgt
     8641 atttccccca cttgaccgac cgcaaacatt taaaaaattg ccactgccaa atctaaaatg
     8701 ccgtctgaaa gacttttcag acggcatttt gcggagtctt taaaacagag aatccaactg
     8761 ctgctgtttg gaaccagtat tactcggaag caccggcgtt tcctgcatat cttggcggac
     8821 ttcgtcatcc gccgcctgcc gtccgccttc tgccgcgccc ccgtcatctt cttttgcccg
     8881 tcgggaaggt tgcggcgcaa taccgctgtt gtccagcgtc aagcccggat cggttaccat
     8941 acgttccttc atatagtatt cgccattgct gctgaccaca ccttcaggca ttttcatccc
     9001 cttgccctgc tttcctttca acgcaaaacg catatagtcc acccaaaccg gcaccgcaat
     9061 cgtaccgccg tagccgacac gccccatact cttaggtttg tcgaagccga tatatacggc
     9121 agtaaccaca tcagggttaa aaccgacaaa ccacgcatcc ttattgtcat tggtcgtacc
     9181 cgttttaccg gcaatatccg ttcttcccaa cgcagctgcc ccccttgccg taccgacacg
     9241 gaccacatcc tgcataatct tatacataat ataggcattg cgcggatcga ttgcctgagg
     9301 cgcattttgc ccggcctcca aaggttgcat ttgggcgcgc aacctgccgt ctctgtcata
     9361 aatcttatcg attacgtgcg aagaaaccct atatccgccg ttcgcaaata cgctatatgc
     9421 ctccgccact ttcaacggcg ttgtctcgcc cgtacctaaa gccatagaca ggcttgccgg
     9481 cagctcggac gacctgaagc cgaaacgccg gatatactgt tgcgcgtaac cgacaccgat
     9541 agacatcaaa atacggatgg aaaccatatt cttggaagcc gtcagagcct gtctcaaagt
     9601 aatgtagccg gaatatctgc cgtctgaatt tttaggtgtc caaaccgaac cgttcggccc
     9661 tttccccggc agggaaatcg gcgcatcgtt aaccactgtg gacgcggtca tccccttaga
     9721 taatgccgcc gaatagacaa acggcttaaa ggtcgaaccc ggctgccgca ttgcctgaac
     9781 ggcacgattg aatgttttgc tgtgaaaatc ataaccgccg accagcgcgc gcacagctcc
     9841 ggtttttgca tccagcgaaa ccaaagcccc ctgcagcaac ggctcttgaa ccaccgccca
     9901 acgcccgccg ttgtttttga cacggatgac cgcgcccctg cggatacggt cctcccccat
     9961 tttttcatta ttgaccgcgc gggccgcaaa acccaaggcg cgcctgtcaa gcgtaacccg
    10021 cctgccgccg ggcagctgta tgacgacatt tttcttttta gtcacatcca acacaacggc
    10081 gggaaccatt ttatcgacgg tatagagtcc cgacagatac tggctgacag tctcctcgac
    10141 atcttcactc ttactcaaat cgatatagtt ttccgcaccg cggtagctgc tgccgcgatc
    10201 gaaattccgt agagccttgc gcaatgcctc ggttgccacc ttctgatgat cagcgcggac
    10261 cgtggtataa accttaaaac cctgcgtata ggcatcttca ccgtatttct catacagttc
    10321 ctgacgcacc atttccgcca catataaagc actctgatcg attttctgaa caaaccgctc
    10381 gtaatgcagt tcctcattca acgcctgatc gcgctgttgc acggtaatca tcttctcctc
    10441 gagcatattg ttcaaaatat acttctggcg caacttggca cgttccggat taacaatcgg
    10501 attataggca gacggagcct tgggcagtcc cgcaagcatg gcggcttccg ccaaagtcaa
    10561 atctcggaca ttcttattga aatagatttg cgcggcagat gcaaaaccat aggcgcgctg
    10621 accgaggtaa atctgattga aatacaactc gaggattttg tctttgctta aagactgctc
    10681 gattttatag gcaagcaaca cctcattgaa tttgcgtgtg aacgtttttt cactgctcaa
    10741 ataaaaattt ttcgccacct gctgcgtaat cgtactcgca cccgactgca cgctgccgga
    10801 cacgacattg ccgacggcag cgcgggcaac accccaaaca tccacccccc aatgccggta
    10861 aaagcgttta tcctcggcgg cgataaccgc attccgcaac acctctggga aatcgccgat
    10921 ttttgtaaat tcgcgccgct gctccccata cataccgatg acttccccat ccgccgaata
    10981 aatagtcaac ggcattttag gctggtaatg ctgcaaagaa tccaaagacg gcagtttcgg
    11041 atacgttacc aaaatagcaa tggcaaccaa acccactcca aatacacaaa acccaaaaac
    11101 caaaccaaaa caagtcgtta aaatcttttt aatcatagct gaataataat ttaccattat
    11161 tggtattaaa taaagtaaaa tagcaaccga tttctacaaa gcacggtttc aatgtgcaaa
    11221 gaacaaggaa tccattacgg ataccgaaac ggttactcac tgtacaaata aagcaggaac
    11281 tttcatcatg cgcttgttta aaagcttgaa aaaccctaaa aaaacagatg ccaagctccc
    11341 taaaaaatct tcgggactca ataaccgcgc ggcaatcggc atcgatatcg accagcattc
    11401 catcaaaatg gtccaattgt caggacgtag tttaaaccaa attcaattgg aaaaatacgt
    11461 cattgccaaa ttaccaaaga atatcattca aggcaataaa gtccaaaatt acgatcaact
    11521 tgttacatat ttgcaacaag cctatgccaa actgggtacg tcgtgcaaaa acatcatcgc
    11581 gtccgtcccg caaaatctgg caaccatcga acaattgacc tacacagaca aagatgcaga
    11641 attagacctg caggggttcg tggagtcctc catctccgaa gtcagctcga tatcgctcga
    11701 agaagccaat tacgactatc aggtcttgtc ccaatcggcc gccggcgaag ctgtgttggc
    11761 cgtcgcatcg agaaaggatg aaatcgaacc cctgattgac gcattcaacg ccgccggtat
    11821 gaaattatcc gcgcttgatg tggacatttt cggacaatac aacgcctacg cgctatggat
    11881 aaaccatttc gcccccgagc ttgcagccga aaaagtcgcc attttcggcg tatatgccgc
    11941 acagacctac gccttggtca tccaagacgg aaaaatccta tacaaacagg aaacctccgt
    12001 cagcgaagaa cagctcaacc aactcatcca gcgcacctat caggtaacag aagaaaaagc
    12061 ggaagaaatc atcaactccc cgcaaaaacc ttccgattac caagaaagcg tggcaaacta
    12121 tttcaaccag cagattaccc aagaaataca aagggtcttg cagttttatt acaccacgca
    12181 gaccgcagac gatatgaccg acatcaagca tatcctgctg accggggaag cggcgcgcca
    12241 ggaaggcatc gcccaaaccg tcgcctcaca aaccaatgca gatgtacaat gcgtccatcc
    12301 cgcgcgttat tttgcggaca acctcaaaac agacaaacaa caattcgaac ttgatgcgcc
    12361 gacactgacc agggcgttcg gtttggcggt acggggatta taattatgaa caatttaatc
    12421 aaaatcaacc tcctccccta cagggaagag atgaacaagc gcaaacagca gcagtttaaa
    12481 acgctgatgt acggtgccgt gctgacgggc gttgccgccg ttgcggcaac ctacctgttt
    12541 atcgacaata tgatcaataa ccagtcggaa agaaacacgc tgctggaaac ctccatcgca
    12601 cacttggata ccgagctgtc ggaaatacaa aagctcaaac aggaaaaaga tgccttcctg
    12661 attaagaaaa acaaagtcga ggagctccag ctcaaacgcc tccaagccgc aaaaatcctc
    12721 gacagcctga atgaggccgt ccccggaagc acctacctga cctcgctgga tgccgttacc
    12781 gccgactctt atcggctcag cggcaggaca tccagcgaca accgcgttgc cgccatgatg
    12841 agggcgatgc ccaataccgg catattcaag caacccgaat tgttaagcat caagaaaaac
    12901 aattcgcatc aagaatttac ccttcaggca acattacaac ccatcgtaaa ggcggccgaa
    12961 tccaaagaga atccggcttc gggaaacgca caggaggcaa actgaatggc ttctaaatca
    13021 tctaaaacca acttggatct caacaacctt cacctgctca accttcctgc caggcttttt
    13081 atcgccctgc tggccgttgc cgccgtgctg gggctcggtt atgccggatt gttcaaaagc
    13141 cagatggaat cccttgagga atacgaagca aaagaaaccg aactgaaaaa cacctacaaa
    13201 cagaaaagta tcgacgcggc cagcctgaac aacctgaggg acgaacttgc ctcaatccgc
    13261 tctgccttcg atatcatgtt gaaacagctg ccgacagatg cagaaattcc caatctggtt
    13321 caagagcttc atcaggcagg ttcgagcaac ggcctgcgct tggacagcgt tatgccccaa
    13381 cctcccgtag atgacggccc catcaaaaga ttaccctatt ccatttccat taccggaaat
    13441 tacgaacaga tcagccaatt tacccgcgat gtcggcagcc tctcccgaat cattaccctt
    13501 gagtcgctga aaatcgccca atctccggaa aacggcggca atcctgacgg caagagcagc
    13561 atcctgaacc tcagtgccat tgccaccacc taccaagcaa aatccgtaga agagcttgcc
    13621 gcagaagcgg cacaaaatgc cgagcaaaaa taacttacgt tagggaaacc atgaaacact
    13681 atgccttact catcagcttt ctggctctct ccgcgtgttc ccaaggttct gaggacctaa
    13741 acgaatggat ggcacaaacg cgacgcgaag ccaaagcaga aatcatacct ttccaagcac
    13801 ctaccctgcc ggttgcgccg gtatacagcc cgccgcagct tacagggccg aacgcattcg
    13861 acttccgccg catggaaacc gacaaaaaag gggaaaatgc ccccgacacc aagcgtatta
    13921 aagaaacgct ggaaaaattc agtttggaaa atatgcgtta tgtcggcatt ttgaagtctg
    13981 gacagaaagt ctccggcttc atcgaggctg aaggttatgt ctacactgtc ggtgtcggca
    14041 actatttggg acaaaactac ggtagaatcg aaagcattac cgacgacagc atcgtcctga
    14101 acgagctgat agaagacagc acgggcaact gggtttcccg taaagcagaa ctgctgttga
    14161 attcttccga caaaaacacc gaacaagcgg cagcacctgc cgcagaacaa aattaagaag
    14221 aggattactc cattatgaat accaaactga caaaaatcat ttccggtctc tttgtcgcaa
    14281 ccgccgcctt tcagacagca tcggcaggaa acattacaga catcaaagtt tcctccctgc
    14341 ccaacaaaca gaaaatcgtc aaagtcagct ttgacaaaga gattgtcaac ccgaccggct
    14401 tcgtaacctc ctcaccggcc cgcatcgcct tggactttga acaaaccggc atttccatgg
    14461 atcaacaggt actcgaatat gccgatcctc tgttgagcaa aatcagtgcc gcacaaaaca
    14521 gcagccgtgc gcgtctggtt ctgaatctga acaaaccggg ccaatacaat accgaagtac
    14581 gcgggaacaa agtttggata ttcattaacg aatcggacga taccgtgtcc gcccccgcac
    14641 gccccgccgt aaaagccgcg cctgccgcac cggcaaaaca acaggctgcc gcaccgtcta
    14701 ccaagtccgc agtatccgta tccaaaccct ttaccccggc aaaacaacag gctgccgcac
    14761 cgtttaccga gtccgtagta tccgtatccg caccgttcag cccggcaaaa caacaggcgg
    14821 cggcatcagc aaaacaacag acggcagcac cagcaaaaca acagacggca gcaccagcaa
    14881 aacaacaggc ggcagcacca gcaaaacaaa ccaatatcga tttccgcaaa gacggcaaaa
    14941 atgccggcat tatcgaattg gctgcattgg gctttgccgg gcagcccgac atcagccaac
    15001 agcacgacca catcatcgtt acgctgaaaa accataccct gccgaccacg ctccaacgca
    15061 gtttggatgt ggcagacttt aaaacaccgg ttcaaaaggt tacgctgaaa cgcctcaata
    15121 acgacaccca gctgattatc acaacagccg gcaactggga actcgtcaac aaatccgccg
    15181 cgcccggata ctttaccttc caagtcctgc cgaaaaaaca aaacctcgag tcaggcggcg
    15241 tgaacaatgc gcccaaaacc ttcacaggcc ggaaaatctc ccttgacttc caagatgtcg
    15301 aaatccgcac catcctgcag attttggcaa aagaatccgg gatgaacatt gttgccagcg
    15361 actccgtcaa cggcaaaatg accctctccc tcaaagacgt accttgggat caggctttgg
    15421 atttggttat gcaggcacgc aacctcgata tgcgccaaca agggaacatc gtcaacatcg
    15481 cgccccgcga cgagctgctt gccaaagaca aagccttctt acaggcggaa aaagacattg
    15541 ccgatctggg cgcgctgtat tcacaaaact tccaattgaa atacaaaaat gtggaagaat
    15601 tccgcagcat cctgcgtttg gacaatgccg acacaaccgg aaaccgcaat acgcttgtca
    15661 gcggcagggg cagcgtgctg atcgatcccg ccaccaatac cctgattgtt accgataccc
    15721 gcagcgtcat cgaaaaattc cgcaaactga ttgacgaatt ggacgtaccc gcgcaacaag
    15781 tgatgattga ggcgcgtatc gtcgaagcgg cagacggctt ctcgcgcgat ttgggcgtta
    15841 aattcggcgc gacaggcaag aaaaagctga aaaatgatac aagcgcattc ggctgggggg
    15901 taaactccgg cttcggcggc gacgataaat ggggggccga aaccaaaatc aacctgccga
    15961 ttaccgctgc cgcaaacagc atttcgctgg tgcgcgcgat ttcctccggt gccttgaatt
    16021 tggaattgtc cgcatccgaa tcgctttcaa aaaccaaaac gcttgccaat ccgcgcgtgc
    16081 tgacccaaaa ccgcaaagag gccaaaatcg aatccggtta cgaaattcct ttcaccgtaa
    16141 cctcaatcgc gaacggcggc agcagcacga acacggaact caaaaaagcc gtcttggggc
    16201 tgaccgttac gccgaacatc acgcccgacg gccaaatcat tatgaccgtc aaaatcaaca
    16261 aggactcgcc tgcgcaatgt gcctccggta atcagacgat cctgtgtatt tcgaccaaaa
    16321 acctgaatac gcaggctatg gttgaaaacg gcggcacatt gattgtcggc ggtatttatg
    16381 aagaagacaa cggcaatacg ctgaccaaag tccccctgtt gggcgacatc cccgttatcg
    16441 gcaacctctt taaaacacgc gggaaaaaaa ccgaccgccg cgaactgctg attttcatta
    16501 ccccgaggat tatgggtacg gccggcaaca gcctgcgcta ttgatgcgtc aaaataaggg
    16561 catatgtttt acggcatatg ccctttcttt atgctttttg ccgcgaccga aatgccgtca
    16621 ttcccgcgca ggcgggaatc cagtccgttc agtttcggtc atttctgata aattcctgtt
    16681 gcttttcatt tctggattcc cacttttgtg ggaatgacgg cggaaggggt aaatcctcgc
    16741 aatccaaagc ctcgtcattt ccacaaaaaa cagcaacccg gaacagcaac ttaaaacccc
    16801 gtcattcccg cgcaggcagg aatccaactt gtccggttcc ggttgttttt cgtctcgtaa
    16861 cttttgagcc gtcattcccg cgcaggcggg aatctaggtc tgtcggttca ggaacttatc
    16921 ggataaaacg gtttctccaa ccctgcgttc tagattccca ctttcgtggg aatgacggga
    16981 tatgggtttc cgtgcggacg tgttcggatt tccgcctgcg cgggaatgac ggcgacagat
    17041 gcccaacggt ctttatagtg gattaacaaa aatcaggaca aggcgacg
//
LOCUS       DAWFAD010000033        16597 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWFAD010000033 DAWFAD010000000
VERSION     DAWFAD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16597
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.033"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(144..566)
                     /locus_tag="WGO49_000797"
     CDS             complement(144..566)
                     /locus_tag="WGO49_000797"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ0305928.1"
                     /translation="MSIMQVEVVSSEQNIYSGEASFVVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(577..1974)
                     /gene="atpD"
                     /locus_tag="WGO49_000798"
     CDS             complement(577..1974)
                     /gene="atpD"
                     /locus_tag="WGO49_000798"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ0305929.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(2012..2887)
                     /gene="atpG"
                     /locus_tag="WGO49_000799"
     CDS             complement(2012..2887)
                     /gene="atpG"
                     /locus_tag="WGO49_000799"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ0305930.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(2912..4459)
                     /locus_tag="WGO49_000800"
     CDS             complement(2912..4459)
                     /locus_tag="WGO49_000800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ0305931.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(4470..5003)
                     /locus_tag="WGO49_000801"
     CDS             complement(4470..5003)
                     /locus_tag="WGO49_000801"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ0305932.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(5008..5478)
                     /locus_tag="WGO49_000802"
     CDS             complement(5008..5478)
                     /locus_tag="WGO49_000802"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ0305933.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(5549..5785)
                     /gene="atpE"
                     /locus_tag="WGO49_000803"
     CDS             complement(5549..5785)
                     /gene="atpE"
                     /locus_tag="WGO49_000803"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ0305934.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(5842..6708)
                     /gene="atpB"
                     /locus_tag="WGO49_000804"
     CDS             complement(5842..6708)
                     /gene="atpB"
                     /locus_tag="WGO49_000804"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ0305935.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(6698..7051)
                     /locus_tag="WGO49_000805"
     CDS             complement(6698..7051)
                     /locus_tag="WGO49_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ0305936.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAIWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            complement(7419..7757)
                     /locus_tag="WGO49_000806"
     CDS             complement(7419..7757)
                     /locus_tag="WGO49_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305937.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            complement(7892..8752)
                     /locus_tag="WGO49_000807"
     CDS             complement(7892..8752)
                     /locus_tag="WGO49_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ0305938.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     VALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            8911..9480
                     /locus_tag="WGO49_000808"
     CDS             8911..9480
                     /locus_tag="WGO49_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ0305939.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            complement(9542..10405)
                     /locus_tag="WGO49_000809"
     CDS             complement(9542..10405)
                     /locus_tag="WGO49_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305940.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAEKKE
                     IVFGTTVGDFGDMVKEQIQAELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(10564..11250)
                     /locus_tag="WGO49_000810"
     CDS             complement(10564..11250)
                     /locus_tag="WGO49_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0305941.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIVRKVLLNEARTGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            complement(11252..11989)
                     /locus_tag="WGO49_000811"
     CDS             complement(11252..11989)
                     /locus_tag="WGO49_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0305942.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            12366..14213
                     /locus_tag="WGO49_000812"
     CDS             12366..14213
                     /locus_tag="WGO49_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0305943.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETAGLSAS
                     VPTRPAEPERKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQSLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLY
                     KQAAATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFRNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVMGAQSRVGAQGLMQVMPATAHEIA
                     GKIGMDATQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTPL
                     EGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            14502..14714
                     /locus_tag="WGO49_000813"
     CDS             14502..14714
                     /locus_tag="WGO49_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ0305944.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            14846..15292
                     /locus_tag="WGO49_000814"
     CDS             14846..15292
                     /locus_tag="WGO49_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ0305945.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            15313..15462
                     /locus_tag="WGO49_000815"
     CDS             15313..15462
                     /locus_tag="WGO49_000815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305946.1"
                     /translation="MPSEARIAGSDSIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(15491..15883)
                     /locus_tag="WGO49_000816"
     CDS             complement(15491..15883)
                     /locus_tag="WGO49_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ0305947.1"
                     /translation="MPTSTKPYVLRVLCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFSGQAHDIWIPVGHVLSLFARETSEGMGFELE
                     EYRPDTPPENTPAETAPRPAKKGLKLVK"
     gene            complement(15955..16560)
                     /locus_tag="WGO49_000817"
     CDS             complement(15955..16560)
                     /locus_tag="WGO49_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0305948.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NYVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
BASE COUNT         3992 a         4389 c         4347 g         3869 t
ORIGIN      
        1 tttcggttag gcggcaggct gccctattga ataccttaaa gcaggctatg cctgccaacg
       61 ccatatccaa acacagtctt taatttaaat ccggaaaata aaaagcacga ccaaacggtc
      121 gtgcttttcc aaaccaaaca agtttatttc ttgtgcgaac ggatatagtc caaagttttg
      181 agctgtgcaa tcgcagcagc caatgcttta tgtgcttccg ccaaagcctt atcgtcttta
      241 gcttgggaaa tgcccgcttc tgcggctttt ttcgcctctt ccgcacgtgc ctgatccatc
      301 tccgcactgc ggacggcaac atccgccaag acagttactt tatcaggctg tacttccaaa
      361 acaccgccgg aaacagcaac caaaacctct ttatcctcgc ccggaacggt caaacgcaaa
      421 gcccccggcc gcaccaaact cataatcggc tcgtgtcgcg ggtaaatacc aagctcacct
      481 tgaacggtcg gaaccactac aaagctggct tcgcccgaat agatgttttg ctcgctactg
      541 accacctcaa cttgcatgat gctcatgccg acctcctcag tttaaggttt tcgctttctc
      601 aaccgcttct tcgatgctgc cgaccatgta gaatgcctgt tcgggcagat ggtcgtattc
      661 gccgttcaag atggctttga agccggcaat ggtatcgcgc agggcgacat atttgcccgg
      721 agaacctgta aacacttcgg caacgtggaa cggttgggac aggaagcgtt ggattttacg
      781 cgcacgcatt acggtcagtt tgtcttcgtc agacaattcg tccataccca agatggcgat
      841 gatgtcgcgc aattctttgt atttctgcag ggtggactgt acgccgcgcg ccacgtcgta
      901 gtgctcttga cccaagacca tcggatccag ttggcgcgaa gtggaatcaa gcggatcgac
      961 tgccgggtaa atacccaaag aggcaatatc gcggctcaat acgacggttg cgtccaagtg
     1021 ggcgaacgtt gttgccggag acgggtcggt caagtcgtcc gcaggtacat atacggcttg
     1081 gatagaagta atagaaccgg tttgggtaga ggtaatacgc tcctgcaaac gacccatttc
     1141 ttctgccaat gtcggttggt agcccactgc agacggcata cggcccaaca atgcggatac
     1201 ttcggtaccg gccagagtgt aacggtagat gttgtccacg aagaacaata cgtcgcggcc
     1261 tttgccgttt tcgtcttttt cgtcacggaa gtattccgcc atggtcaaac cggtcaatgc
     1321 gacgcgcaaa cggttgcccg gaggttcgtt catctgaccg taaaccattg ccactttatc
     1381 caatacgttg gaatctttca tctcgtggta gaagtcgtta ccttcgcggg tacgctcacc
     1441 cacgcctgcg aacacggaca agccgctgtg cgctttggcg atgttgttga tcaattccat
     1501 catgttcacg gttttaccca caccggcacc gccgaacaga cctactttac cgcctttggc
     1561 aaacggacac agcaagtcaa tcactttaat gcccgtttcg agcaattcgg ttgtggaaga
     1621 cagttcgtca aacttaggag cggcttggtg gatggcacga ctcttgtcgg tatcgatcgg
     1681 acctgcctcg tcaacaggcg ttcccaatac atcgacaatg cgtcccaacg tacctttacc
     1741 taccggcaca gtaatgggcg caccggtatt gctcacagtc atgccgcgtt tcaaaccgtc
     1801 cgagctgccc atcgcaatgg tacggactac gccgtcgccc aaaagctgtt ggacttccaa
     1861 agtcagaccg ttttcgtcta atttcaaagc gtcgtaaacg cgcggaatca tgtcgcgtgg
     1921 aaattccacg tcaacaaccg caccgataat ttgtacgatt ttgccttggc tcattatcgt
     1981 atcctaattt ccgtacagga ttcagacggc atcagacagc cgccgcacct gctacaattt
     2041 ctgacaattc cgtggtaatc gcagcttgac gcgatttgtt atataccaaa cgcaactctt
     2101 tgatggcatt gcctgcattg tctgttgcag ctttcatggc aaccatgcgg gctgcctgtt
     2161 cggatgccat attgtcgctc aacgcctgat aaaccacaga ctctaaatag cggcgaacca
     2221 gatattccaa cactgcaagt gcagtcggtt cgtagcggta ttcccagctg aacggtgatt
     2281 tgggagctga atcgccaatc acgttctcac cgataggcag caatacttcc attctcggtt
     2341 cctgacgcat ggtattgaca aaacccgaat acaccagatg gattctgtca atttcatgtt
     2401 tctcataccg ttggaagagt tctgtcaaag gtccgagcag catttccatt tttggggtat
     2461 cgcccaaatt tacggcactg gcaaccacat tcagaccaat gctctgacac gccatcagac
     2521 ctttactgcc aaggcatacg atttcctctt caataccttg attccgatac tcttgaactt
     2581 gtgccaaaaa ctttttcagc acgttggcgt tcaaaccgcc acacaaaccc ttatcagacg
     2641 taatcaaaat aaaaccgaca cgtctgattt cccgatgaga ttccagcaac ggaataccat
     2701 gatcggtatt ggtttgcgca agatggctca tcaccatacg cactttttcg gcatacggac
     2761 gcgccaaacg catccgttcc tgagtcttcc gcattttaga ggttgacacc atctgcatcg
     2821 ctttagtgat cttttgggta ttctgaacac tgcggatttt ggtgagaatc tcttttccta
     2881 ctgccatttc agactccttt cacttcaagc cttatgcctg ataggcgtaa gaagatttga
     2941 aggatttcat ggctgcttca agcgttttct cgctctcgtc ggacattgca cctgaagcat
     3001 tgacggcttc caaaacttcc ggatgttggg tacggacaaa gctcaaaaat tcagattcaa
     3061 aagccagagc tttggcaacc ggaacatcag aatacgaacc gttgttgatt gcccaaaggg
     3121 tcaaagccat ttcagccgta ttcaacgtac tgaactgttt ctgtttcatc agttcggtta
     3181 cgacttcgcc atgctccaat tgtttgcgcg tagcttcatc caaatcggat gcaaattgcg
     3241 agaacgccgc caattcacga tattgcgcca acgccaaacg gataccgcca cccagctttt
     3301 taatcacttt ggtttgtgca gcaccgccta cgcgggatac ggaaataccg gcattgattg
     3361 caggacggat accggcgttg aagaggtcgg tttccaagaa aatctgaccg tcggtaatcg
     3421 aaatgacgtt agtcggaacg aaagcagata cgtcgcccgc ttgggtttcg ataatcggca
     3481 aagcagtcag agaaccggtt ttgcctttta cttcgccgtt ggtcaatttc tccacttcgt
     3541 gttcattgac acgtgctgca cgttccaaca gacgggagtg caggtagaac acatcgccgg
     3601 gataggcttc gcggccgggc ggacggcgca aaagcaggga aatttgacgg taagccacag
     3661 cctgtttgga caaatcgtca taaacaatca aggcatcttc gccgcggtcg cggaagaatt
     3721 cacccatcgt acaaccggag taaggtgcga tatattgcaa tgccgccgct tcagatgcag
     3781 ttgcagcaac cacaatcgtg tgttccatcg cgccatgctc ttccaatttg cggaccacgt
     3841 tggcaataga agatgctttt tgaccgatag cgacataaat acagataacg cccgtacctt
     3901 tttggttgac gatggcatcc aatgctacgg ccgttttacc ggtttgacgg tcgccaataa
     3961 tcaactcacg ctgaccgcga ccgacaggaa ccatagagtc aatcgccttc agaccggttt
     4021 gcatcggctg gtcaaccgat ttgcgcgcaa tcacgcccgg tgcgattttt tcgatagggg
     4081 cggtcaaagt tgtattaatc gggcctttgc cgtcaatcgg acgacccaat gcatcaacga
     4141 cgcgtccgac cagttcgcgt ccgaccggca cttccaagat acgaccggta caagtaaccg
     4201 tgtcgccttc tttaatatgt tcgtactcgc ccaacactac ggcgccgacg gagtcgcgct
     4261 ccaggttcat cgccaagccg aaagtgttac ccgggaattc gagcatctca ccttgcattg
     4321 catctgacaa accatggatg cgaacgatac cgtcagttac cgaaatcacc gtaccacggg
     4381 tacgcacttc ggcatttaca gacagatttt cgatcttggc tttaatcaaa tcgctaattt
     4441 cagcaggatt aagctgcatg aaaactctcc taattcgtca tagtcgtgta caaggcactc
     4501 aatttgcctt gtacagacaa atccaaaacc tgatcaccca cttcaacttt tatgccgcca
     4561 atcagctccg gttcgatttc gacagagatt ttcagctcgc tgtcgaaacg cttattcagc
     4621 atttgcgcca actcgccgac ctgtttgtcg gtcaacggat aggcactgta aatgacggca
     4681 gatttgatat ggttgaatga taaggtcaag tcttgatatt gagcatatac ttccggcaat
     4741 atcgacaaac gtttctgccc ggccaagacg ataacaaagt ttttcaactc cttgtctttc
     4801 aaaccgacca aatcgatgag gatatctgct ttttctgaag cattcgtttc aggacggtca
     4861 atcaatgaag ccaccttccc ttcctgaaca accgccgcaa gtttttccag tccgcccaac
     4921 caagactcaa tttggctttt ttcctgagcc agaccgaaca atgcctttgc ataaggtctg
     4981 gcaatcgttg cgaactctgc cataagatta cagctcctgt ttcagggtat cgagcagttt
     5041 tgcgtgtttg gaagcatcga cttcgctgcg caaaatagat tcggcacctt tgacagccaa
     5101 cacggcaacc tgctcgcgca gggattcgcg tgcgcggaac aattcctgct ccacatcggc
     5161 ctttgcctga gctgcaatgc gcgccgcctc ggaagaagcc tgttctttgg cttcttcgac
     5221 aattttggcg gcacgttttt cggcgttggc aaccatttcg gaaacctgat tacgcccttc
     5281 tgccaagagt tctgcaacct ttttttcagc ctgctcgaaa tcgcttttac cacgctcggc
     5341 ggcagccaag ccttcggcga cttttgcggc acgctcatcc aaagcttttg caatcggcgg
     5401 ccacacgaat ttcatggtaa accataccaa accgaaaaag acgatgattt gagcgaataa
     5461 tgttgcattg atattcacgt tacttaacct tcgtactggg gttaatcaaa caggctgcgc
     5521 ctgtacggaa cggacgaatc cgtcctgatt atgcacctgc aaacgggtta acgaaggcga
     5581 acagcagtgc aatggcgaca ccaatcaaga atgcggcatc aatcaaaccg gcaatcagga
     5641 acagtttggt ttgcagcgga ccgatcagtt cgggctgacg ggcagaagac tccaaatatt
     5701 tagaaccgac cattgcgata ccgatagagg cacccaatgc acccaatgca acgatcaaac
     5761 cacatgcgat agcaatcaaa cccattttaa actccttaaa gaaacaaagg ttaaactaca
     5821 aaaacaaact acttaggaaa atcagtgcgc atcatgtgcc tgtccgatat agacgaacgc
     5881 caacgccatg aaaataaacg cctgcagggt aatcaccaaa atatggaaaa tcgcccatgc
     5941 caaaccggca ataatgtgga atacaaacag aatcggatcc atgacttcga cgctgccgga
     6001 agccgcccaa gcaccgccaa gcaaggctat caacaagaat accaattcgc ccgcatacat
     6061 attgccgaac aaccgcatac cgtgggatac ggttttagaa agaaactcga ccaaattcaa
     6121 cagaaagttc gcaggtgcga gttttgcacc gaacggcgcg ctgaacaact cgtgaaacca
     6181 gccacccaat cctttgattt tgatgttgta atagatacaa atcagcaaca cgccgacagc
     6241 gagtgccaaa gtggtgttca aatcggcagt cggtacgaca cgcagcaggg cgtgatggtt
     6301 gccggtaatg ccctgccata ccatcggcag caaatcgacc ggcagcatat ccatcgcgtt
     6361 catcagaaaa atccagacaa acagcgtcag acccaacggc gcgacggctt ttctagactt
     6421 ttcgttgtga atgatgctct tacacatatc gtccacaaac tcaaacaaga tttccactgc
     6481 ggcctggaaa cgtccgggaa cgcctgccgt cgcttttttt gcaccgcgcc acaacagaaa
     6541 gctgccgatt acgcccaaca ggacggcaaa aaagacggca tcaaggttaa taaacgaaaa
     6601 atcagcaatg tttttcagtc cctgaccctg agtaacatcc gacaaactgg tcaagctctg
     6661 caagtggtgc ttgatgtagt cggcagcggt aatggtttca cctgccataa tctttcactc
     6721 tcaacaatac taaaaaaacc aaatggctga caccgagcag ccccatcaga aacggggcga
     6781 acaccagcga ttgatgccat attgcaaata cggcaagcat ggacaacagc gacagcacta
     6841 cttttaaaat ctctccgaag acgaacatcc tgctttgcag gaaggggttt cccctgaaaa
     6901 gttttaaaag taaaactgca acaaacgtgg gaagcaggta ggacaaaccg ccaccgaccg
     6961 ccgaaaggaa tccggcaaaa ccccatacag caaaggcaac tgcggcgcat atggacaata
     7021 cggcggattg taggatgata atctgcttca taaagggaat gtttccgcct cggatttggg
     7081 gcgcggctaa tataatttag aagccttatt acgtcaagcg acagttaatc tttgtgaaac
     7141 aacgtatccc aatccgccgc gctcgccgcc tgaataacgg cgacaggtgt cattctaaca
     7201 cacattacat ataattacag gatattaagg agtttgtccg caatttcttt acatttttaa
     7261 tgttcttacg tgatttgttt gctttacgtg gaaataataa aaaatcaacg cgaaattgta
     7321 gcagtttatc ggtcggattg tcggcagttt ggggaatttg ctcaataaat aaaaggtcgt
     7381 ctgaaaatat tttcagacga ccttttccga ataaaggatt agcaacggcc tgccgcttta
     7441 agcaaagcat tgcattgact tttgcctttg tgcgttccgc ctcccaaaca aattgcatcg
     7501 gaagtggtaa cgccgattgt gctgattaca ctggtaacat agcattggct cacgcgctta
     7561 cccacagttg cggtaaagtt gatgcgtatg ccttcattgt tgcggttgct gatttttacg
     7621 gcatttgggc tgacgcccaa ggcaaacgcg gcacgttcct gaagtttctg gtcggaaacg
     7681 gttacattat tgattgagcc gcaacctgct aatgccaacg caacgaacgc agccgaaacg
     7741 atgatgcgtg tgttcataat ttcctcgaaa attaaaaatg aaaacaggaa aacgattctt
     7801 acgtgaagca gaaaaatgtc aatagaatta tatttcccac ttaaaatctg gaaagctgtt
     7861 ctctatattt cagacggtat atcccgcaaa attaaggtcg gtaatctatg cccaactgct
     7921 tcaagatatg gtcgaaggtt tcgggcgtgt cgaaatgcag gatgattctg ccttttttat
     7981 gattggcggt tttgatttcg gcgttgacac ccagtttttc agtcagcaaa tcattcaggc
     8041 ggccgatgtc ggcggcggca gtctttttgg gctcgggacg tttgttttga agggcgacct
     8101 ggctgcggcg ttcgacttcg cgcaccgacc agccgttttt gacggccttt tgcgccaatt
     8161 cgagctgttc gacgacgggc agggtcagca atgcgcgggc gtgccccatt tcgaggcggc
     8221 gttggtaaag catttcctgc acgggttcgg gcaggcttaa aaggcgcagg ctgttggaaa
     8281 tcgcgcttcg gcttttaccg acggcttggg cgatggtttc gtgggtcagc ccgaactcgt
     8341 cggcaaggcg tttcaagcct tgtgcttctt cgatggggtt gaggttttcg cgctggaggt
     8401 tttcgatcaa acccattgcc aacgcggttt cgtcgctgat ggttttgata acggcgggga
     8461 tttcggtcag gccggcaatc tgtgcggcac gccaacgccg ctcccccgca atcagttcgt
     8521 atcgggacag tccgtgttcg cgcacgatga cgggctgtat cacgccttgc gccttaatcg
     8581 aatccgccag ctcctgcaag gcttcgtcat cgatttgaac acgcgcctga tagcggccgg
     8641 gccggatatc tttaaccgca accgtggtca atcggtcgcc gctgctgttg tctgcgccgt
     8701 tggcgagcag cgaatccaaa ccgcgcccca atccgccttt tacttttgcc ataccgccct
     8761 cccgtgccta tttcagatag gatgttaaat cggatatttt atcggatatt gggtgttgcc
     8821 gacaatttgt atccgtgttt atcggatttc tgttttttca ctataatagc cgatttgccg
     8881 ttgcaggcgg ttttatggga aaggcggatg atggtacggc gtttgataat cggcatcagt
     8941 ggggcgagcg gtttccaata cggcgtgaag gctttggaac ttttacgcgc gcaagatatc
     9001 gaaacgcacc ttgtggtatc gaaaggtgcg gagatggcgc gcgcttcgga aacggcttat
     9061 gcgagagatg aggtatatgc cttggcggac ttcgtgcatc cgatcggcaa tatcggggcg
     9121 tgcattgcca gcggtacgtt taaaacggac gggatgctgg tcgccccctg ttcgatgcgg
     9181 acgcttgcct cggtcgcgca cggcttcggc gacaacctct tgacgcgtgc ggcggatgtg
     9241 gttttgaagg aaaggcggcg gctggtgctg atggtgcgcg aaacgccgct gaaccttgcc
     9301 catttggaca atatgaagcg ggtaacggaa atgggcggcg tggtgtttcc ccctgttcct
     9361 gcgatgtacc gcaaaccgca gacggcggac gacatagtgg cgcacagtgt tgcacacgct
     9421 ttgtcgctgt tcggaatcga tacgccggat tcggcggaat ggcagggaat ggcggattaa
     9481 aggacaaaaa tgccgtctga acacggatac ggttcagacg gcatcatttt atacgactgc
     9541 cttatttggc tgcgccttca ttccatgcgg caggggattt gtagccctcg aagcgtttgt
     9601 gcgcgtaggc tttgaacgcg tcggagttat aggcctcggt tacgtcttta agccattggc
     9661 tgtctttgtc ggcggttttg acggcagacc agttgacata ggcaaagctc ggttcttgga
     9721 acagggcttc ggtcagcttc atgccgctgc ttatggcgta gttgccgttg acgacggcaa
     9781 aatccacgtc ggcgcggcta cgcggcagtt gcgcggcttc aagctcgacg attttgatgt
     9841 ttttcaggtt ctcggcgatg tccgctttgg atgcggtcag cggattgatg ccgtctttga
     9901 gtttgatcca acccagttcg tcgagcatca ccaagacgcg ggcgaagttg gacgggtcgt
     9961 tgggcgcgga tacggtgctg ccgtctttga cttcttccag cgatttcagc ttgcccgggt
    10021 acagtcccaa aggcgcggtc ggcacttgga agacttcggt gatgtccaga ttgtgttctt
    10081 ttttgaagtc gtcaagatag ggtttgtgtt ggaagacgtt gatgtccaac tcgccctcag
    10141 ccaatgccag attcgggcgt acatagtcgg taaactcgac cagtttgacg gtgtagcctt
    10201 ttttctccag ctcggcttgg atttgttctt tgaccatatc gccgaagtcg ccgacggtcg
    10261 tgccgaagac gatttctttt ttctccgcgc cgttgtcggc ggcggcagaa gcggatgcgg
    10321 cgggcgcgct gtctttttga ccgccgcagg cggcgaggat gagcgcgagt gcggcggcgg
    10381 aaagggtttt gaagaaggtt ttcatatttt ctcctgatgt tgtggcagtt tcaaacaaaa
    10441 atgacgggca gggagtcctg ccgtccggat tcggcgttca gacggcattt gccgcgaaca
    10501 gggggatttt atagcatttt tcggatagcg gtgggggttt tggcgttcag acggcattcg
    10561 ggtttaacgt ttgtcgagtt tccgcgccaa cgcgttgccg gtgctttgaa tcaggatgac
    10621 cagcagcacg aggagggcga cgatgaagac gatgacttcg gtttggtagc ggtagtagcc
    10681 gtagcggatg gcgaggtcgc ccaagccgcc gccgcctatc atccctgccg ccgcgctgta
    10741 tgacaaaagc ccgatggcaa gcacggtaat gctggaaacc atgcccgtgc gcgcttcgtt
    10801 taagaggact ttgcggacga tggcaaccgg cggcgcaccc atcgcggcgg cggcttcaat
    10861 cacgcctttg gggacttcgc gcaggttttg ttccaccagt cgggcgaagt aaaacagacc
    10921 ggacacgctc aataccagcg aggcggcaac tggaccgatg gtgctgccga caatggcgcg
    10981 tgtggcgggt atcatcgcaa tcatcaaaat gacgaagggg aaggcgcgca tgaggttgac
    11041 gaggttgtcg agcaggaaat ttaccggctt gttgtaatgc agttggcggc tggaggttac
    11101 gaagagcaac acgcccagca acgtgccgaa aacgacggcg aatgtggtgg acaaaccgac
    11161 cattacgaag gtttcgccca atgcgcggac gatttcgcct ttcataccga tgatgttgga
    11221 aacggcttgt tggaaggtca ggtctgccat ttcagtcctc tctgatgagt tcgcgcccga
    11281 tgtcggatcg ggcgtggatt tggttgccgc gtacttcgac gatttcgacg actttgcctt
    11341 tatccaacag ggcggcgcgg tcgcacagtc gtctgataac gctcatttcg tgggttacga
    11401 tgacgatggt tacgttgaat cgtttgttga tgtcttccaa acattccaag acgctgcgcg
    11461 tggtggcggg gtcgagggcg gaagtgggtt cgtcggcgag gatgacttgg ggtttgggcg
    11521 cgagtgcgcg ggcgatgccg acacgctgtt tctgcccgcc tgaaagctgg gcgggatagt
    11581 ggccggcgcg ttcggtcagt ccgacgattt cgagacattc ggcaacgcgc gctttgattt
    11641 tttcagacgg ccatccggcg atttccaatg ggaaggcgac gttgccggcg acggtgcggt
    11701 tgttcaacag gttgaactgc tgaaacacca tgccgatatt ctgccgagcc tgacgcaatg
    11761 cggcggcatc gagcgcggtc agctcttgtc cgcagacgtt gaccttgccg ctgtcgggac
    11821 gttccaacag gttaatcagg cgcaacaggg tggatttgcc tgcacccgaa taacccatca
    11881 gcccgaagat ttcaccgtcg cggatttcga ggctggtcgg ctcgacggcg gcaaaacggg
    11941 tcttgtcgcg cgtttggtaa tgcttggaaa ccttgtccaa aataatcatt gtctttccca
    12001 tacaacaaag cccgatgtcg gacacaacgg gcgcggaaga taaagctgaa attgtcggaa
    12061 cgctttagct gttatgcccg caagctgtgt caaatcggca ggttaatttt cgtaggatat
    12121 tatcgggaaa gcgttttttt gtcaataagg caggaagcgg gcaaccattt cggacaatgc
    12181 cgtctgaaac aggcaaaggc agcagttcgc accaaaacgg caaatgattg aaaaatataa
    12241 attatgtcag aaacattcca aagccggtgc agtttcggca tataattcat gcaaacgcct
    12301 gttcagacgg cattttgtct tttccaaccc tgaccgttca gggttccgat tcttaaggaa
    12361 atccgatgta cctaccctct atgaagcatt ccctgccgct gctggcggcc ctggtgcttg
    12421 ccgcgtgttc ttcgacacac gcgcccagca gcaagaccct ggcagacaat atagaaactg
    12481 ccggtctttc ggcaagcgtt cccacccgcc ctgccgaacc cgaaagaaaa acgctggcag
    12541 attacggcgg ctacccgtcc gcactggatg cagtgaaaca gaaaaacgat gccgccgtcg
    12601 ccgcctattt ggaaaacgcc ggcgacagtg cgatggcgga aaatgtccgc aacgagtggc
    12661 tgaagtcttt gggcgcacgc agacagtgga cgctgtttgc acaggaatac gccaaactcg
    12721 aaccggcagg gcgcgcccaa gaagtcgaat gctacgccga ttcgagccgc aacgactata
    12781 cgcgtgccgc tgaactggtt aaaaatacgg gcaaactgcc ttcgggctgc accaaactgt
    12841 tggaacaggc agccgcatcc ggcttgttgg acggcaacga cgcctggagg cgcgtgcgcg
    12901 gactgctggc cggccgccaa accacagacg cacgcaacct tgccgccgca ttgggcagcc
    12961 cgtttgacgg cggtacacaa ggttcgcgcg aatatgccct gttgaacgtc atcggcaaag
    13021 aggcgcgtaa atcgccgaat gccgccgccc tgctgtccga aatggaaagc ggtttaagcc
    13081 tcgaacaacg cagtttcgcg tggggcgtat tggggcatta tcagtcgcaa agcctcaatg
    13141 tgcctgccgc cttggactat tacggcaagg ttgccgaccg ccgccaactg accgacgacc
    13201 aaatcgagtg gtacgcccgc gccgccttgc gcgcccgacg ttgggacgag ctggcctccg
    13261 ttatctcgca tatgcccgaa aaactgcaaa aaagcccgac ctggctctac tggctggcac
    13321 gcagtcgcgc cgcaacgggc aacacgcaag aggcggaaaa actttacaaa caggcagcag
    13381 cgacgggcag gaatttttat gcggtgctgg cgggggaaga gttggggcgc aaaatcgata
    13441 cgcgcaacaa tgtgcccgat gccggcaaaa acagcgtcct ccgtatggcg gaagacggcg
    13501 cgattaagcg cgcgctggtg ctgttccgaa acagccgaac cgccggcgat gcgaaaatgc
    13561 gccgtcaggc tcaggcggaa tggcgttttg ccacacgcgg ctttgacgaa gacaagctgc
    13621 tgaccgccgc gcaaaccgcg ttcgaccacg gtttttacga tatggcggtc aacagcgcgg
    13681 aacgtaccga ccgcaaactc aactacacat tgcgctatat ttcgccgttt aaagacacgg
    13741 taatccgcca cgcgcaaaat gttaatgtcg atccggcgtg ggtttacggg ctgattcgtc
    13801 aggaaagccg cttcgttatg ggcgcgcaat cccgcgtagg cgcgcagggg ctgatgcagg
    13861 ttatgcctgc caccgcgcac gaaatcgccg gcaaaatcgg tatggatgcc acacaacttt
    13921 acaccgccga cggcaatatc cgtatgggga cgtggtatat ggcggacacc aaacgccgcc
    13981 tgcaaaacaa cgaagtcctc gccaccgcag gctataacgc cggtcccggc agggcgcgcc
    14041 gatggcaggc ggacacgccc ctcgaaggcg cggtatatgc cgaaaccatc ccgttttccg
    14101 aaacgcgcga ctatgtcaaa aaagtgatga ccaacgccgc ctactacgcc gccctcttcg
    14161 gcgcgccgca catcccgctc aaacagcgta tgggcattgt tcctgcacgc tgacgtaccg
    14221 atgccgtctg aaacccgccc ggtctttcag acggcatttt tatcccgaac ggcattgacg
    14281 gcgaaccata aatataagac aatccgaaaa ttgtttttcc tgctttttca agcagcttga
    14341 cacggcacaa gccgacccgt taggaggtga tgtttccgtc acggcgcgta tcccgccgcc
    14401 gcaaggcaca gcgatacggt aaaccttcaa caccgtctgc cctacccttt ccaccgatat
    14461 gatgggcaga tgaaacaacc gaatttatta aaggaaataa aatgcctgca atccgcgtaa
    14521 aagagaatga accctttgaa gtcgctatgc gccgtttcaa acgcgccgta gaaaaaaccg
    14581 gcctgctgac cgagctgcgc gcccgcgaag cctacgaaaa accgactacc gaacgcaaac
    14641 gcaaaaaagc ggcagccgta aaacgcctgc aaaaacgcct gcgcagccaa caactgccgc
    14701 ccaaaatgta ctaaacgttc aagtacagat tacaggtcag ccctgtgata tgaggacaca
    14761 ccgcaagacc tgctctgcgg tgtgttttgc ttttcagacg gcatcgaaac ccgccgtttc
    14821 catccgacat cccagcgagg acattatgag cctgaaaatc cgccttaccg aagacatgaa
    14881 aaccgcgatg cgcgccaaag accaagtttc cctcggcacc atccgcctca tcaacgccgc
    14941 cgtcaaacag tttgaagtgg acgaacgcac cgaagccgac gatgccaaaa tcaccgccat
    15001 cctgaccaaa atggtcaaac agcgaaaaga cagcgcgaaa atctacactg aagccggccg
    15061 tcaggatttg gcagacaaag aaaacgccga aatcgaggta ctgcaccgct accttcccca
    15121 aatgctctcc gccggggaaa tccgtactgc ggtcgatgcc gccgttgccg aaaccggcgc
    15181 ggcaggtatg gcggatatgg gcaaagtgat gggcgtattg aaaacccgac ttgccggcaa
    15241 agccgatatg ggcgaagtca acaaaatcct gaaagccgtg ctgaccgcct gattgcccga
    15301 atatcggaca aaatgccgtc tgaagcccgt atcgcaggtt cagacagcat tttcaatgtc
    15361 ccaatatcga atcggcaggg gcaacacggt ttcgatacac cgaaacgggt tttgccgata
    15421 aacggattcc gtttgcgccc catcggacaa aatgccgtct gaaacacgat tccgttcaga
    15481 cggcatagat ttatttgacc aatttcaagc cttttttggc gggtcggggc gcggtttcgg
    15541 caggggtgtt ttcaggcggc gtatcggggc ggtactcctc caattcaaac cccatacctt
    15601 cgctggtctc ccgtgcgaaa aggctgagga cgtgtccgac aggtatccat atatcgtgcg
    15661 cctgtccgct gaaacgggcg gaaaagctga tccaatcgtt gtcgatttga aggttttgcg
    15721 tggcggttgc gccgatattg agcataattt cgttgtcgcg gacgtactgc atggggacgc
    15781 gcgtgtgttc gttgacccaa acaaggatgt gcggtgtgag gctgttgtcg ctgcaccatt
    15841 cgcagagaac gcggaggacg tagggtttgg tggaagtggg cataatgggt tccgtgttgt
    15901 acgccaaaat aggaaaatgc ctgcaaaacg gtgggttttg caagcatttc ggacttattt
    15961 gcgcatggct ttttcggcgg gtgtcagtgc ttcgataaag gcttcgcgct ggaagatgcg
    16021 ctcggcgtat ttgagcagcg gcgcggcact tttgcccagt ttgacatcgt agtggtcgag
    16081 ccgccacagc agcggagcaa gggcgacatc aatcatagaa aaatcttcgc cgaggatgta
    16141 tttgcttttg ctgaacgaag gggcgagcat cgtcaggccg ttgccgatgg cttcgcgcgc
    16201 ttttgcctgt tccttgttgg tggcggcggg gttttccaaa acttggacgt agttgaacaa
    16261 ttctttttcc atacggtaca gcaccagccg gcccctgccg cgcataacgg gatcaccggg
    16321 cataagctgc ggatggggga agcgttcgtc gatgtattcg ttgatgatgt tggactcgtg
    16381 cagcaccaaa tcgcgctcga ccagtacggg gacctggttg tacgggttca tgacggcgag
    16441 gtcttcgggt ttgttgtaaa tatcgacgtc tttgatttca aaatccatac ctttttcgta
    16501 caaaacgaag cggcagcggt ggctgaaggg gcaggtaatg ccggaataga gggtcatcat
    16561 aataattgtc gctcctgtgt gatgcctaca aaacggc
//
LOCUS       DAWFAD010000034        16541 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWFAD010000034 DAWFAD010000000
VERSION     DAWFAD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.034"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            117..1292
                     /locus_tag="WGO49_000818"
     CDS             117..1292
                     /locus_tag="WGO49_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ0305949.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAIIVVHLAGMPAEMDGIMALAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKTLWEKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAVIGRIQLKRLPEWTARRQENAAKLAESLG
                     KFASIRLVEVADYIEHAQYKFYTFVKPEHLKDGWMRDRIVNELNARKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            1340..3250
                     /gene="pglD"
                     /locus_tag="WGO49_000819"
     CDS             1340..3250
                     /gene="pglD"
                     /locus_tag="WGO49_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ0305950.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TAHPRIMTADEAMLPWHELSVLLDRIRSACDRYDQQAIRTLLINAPTGFAPSDGICDL
                     LWVRETHRKNAV"
     gene            3306..4598
                     /locus_tag="WGO49_000820"
     CDS             3306..4598
                     /locus_tag="WGO49_000820"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ0305951.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     PEIDRAFADIFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDSEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(4650..5486)
                     /locus_tag="WGO49_000821"
     CDS             complement(4650..5486)
                     /locus_tag="WGO49_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ0305952.1"
                     /translation="MIDLHCHSIVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RY"
     gene            complement(5796..6197)
                     /locus_tag="WGO49_000822"
     CDS             complement(5796..6197)
                     /locus_tag="WGO49_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305953.1"
                     /translation="MAIYDLNEIVTSAALGMYKGKWQNMDFEQVLQDIVQETDFTYSW
                     EYGENWAIFCLNEKSVGMLCKQIPFAIIENEKLSQKIYQEYPWILTQQVYHFDTDKIL
                     LNKYVCKLTIGIMSAISQEPISLQDLYVDTV"
     gene            complement(6302..6505)
                     /locus_tag="WGO49_000823"
     CDS             complement(6302..6505)
                     /locus_tag="WGO49_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305954.1"
                     /translation="MDTTLKNKAEAILGEPLLDEPIRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(6483..6716)
                     /locus_tag="WGO49_000824"
     CDS             complement(6483..6716)
                     /locus_tag="WGO49_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0305955.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKAFIAEGLVLRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEE"
     gene            6882..10316
                     /gene="dnaE"
                     /locus_tag="WGO49_000825"
     CDS             6882..10316
                     /gene="dnaE"
                     /locus_tag="WGO49_000825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ0305956.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVWIGNPDAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNVHHAELNPEWLENGDNSGLICLSGAHYGEVGVNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMAERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSLDDYLIKLSNEGLQERMVQLYPDEAERAEKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPNMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRILANQVMTLQTARERYARSLSLALAPHHDIGGLVQLLA
                     AHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            10464..10613
                     /locus_tag="WGO49_000826"
     CDS             10464..10613
                     /locus_tag="WGO49_000826"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0305957.1"
                     /translation="MHDTDSTKGKNGIFQSIGIKTALSLQKYQIDKPGKEIRPCRLKK
                     RPPVR"
     gene            complement(10750..11682)
                     /locus_tag="WGO49_000827"
     CDS             complement(10750..11682)
                     /locus_tag="WGO49_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0305958.1"
                     /translation="MLSQLEYGIAKENRPNRQADRFAGSVLPLAASLTHKPNIMKPIP
                     TDTFQPAILPQAFETEIKSTCTGRIYRIQTATLGEMPSEGYPVLFVLDGEAFFPALFN
                     IMQSLMPNPVTRSNAPCLIVGIGYTTGSVRDLAQRAADYTPPLGDNATADERQQFGQA
                     DRFAAFIDSELTAFLESRYTLNRNETAVFGHSFGALFGLYSLLSHRRFRRHWLVSPSI
                     WWHNRRILDFMPSENRLNGIDVCLNIGALERGSDCKRREERDMAGQAEQMAAELDRHG
                     AAVFFREYPNADHGNVPFYSLTDCVEYLRKAWQR"
     gene            complement(11752..13908)
                     /locus_tag="WGO49_000828"
     CDS             complement(11752..13908)
                     /locus_tag="WGO49_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0305959.1"
                     /translation="MSVFRINMTAATVLAALSSSVFAAQTADLETVHIKGQRSYNAIA
                     TEKNGDYSSFAATVGTKIPASLREIPQSVSIITNQQVKDRNVDTFDQLARKTPGLRVL
                     SNDDGRSSVYARGYEYSEYNIDGLPAQMQSINGTLPNLFAFDRVEVMRGPSGLFDSSG
                     EMGGIVNLVCKRPAKAFQGHAAAGFGTHKQYKAEADVSGSLNSDGSVRGRVMAQTVGA
                     SPRPAEKNNRHETFYAAADWDINPDTVLGAGYLYQQRHLAPYNGLPADANNKLPSLPQ
                     HVFVGADWNKFKMNSHDVFADLKHYFGNGGYGKVGMRYSDRDADSNYAFAGSKLGMKT
                     LAGRPGCNTADDKACAVGLGTEIKQKALAFDASYSRPFRLGNTANEFVIGADYNRFRS
                     TNEQGRTTLYARGGLALNEFRSIPQVDLIANARKGVRGYSHTVATENLDEFGIYGKST
                     FHPADGLSLIGGGRLGHYKIELGEGKTLHKASKTKFTGYAGAVYDLNDNNSLYLSLSQ
                     LYTPQTNLDADGKLLKPRQGNQFEVGYKGSYMDDRLNARVSFYRMKDKNAAAPLNPNN
                     KKTRYAALGKRVMEGVETEISGAVTPKWQIHAGYSYLHSQIKTASNSRDDGIFLLMPK
                     HSANLWTTYQVTPELTIGGGVNAMSGITSSAGMHAGGYATFDAMAAYRFTPKLKLQIN
                     ADNIFNRHYYARVGGANTFNIPGSERSLTANLRYSF"
     gene            14068..14727
                     /locus_tag="WGO49_000829"
     CDS             14068..14727
                     /locus_tag="WGO49_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ0305960.1"
                     /translation="MTTPKLIIFDWDGTLADTTQPIIDTMRRSFAECGFPPPEAERVR
                     SLIGYSLPEIIRDLLEMPSETAVADIARTYSAHYLNPNNRNMTLFPDALPCLDKLKAQ
                     GFRLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGICGELGFDPK
                     EALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLLSAPHLAVLDGLSELPGFLARHY
                     A"
     gene            complement(14814..15782)
                     /gene="ispH"
                     /locus_tag="WGO49_000830"
                     /gene_synonym="lytB"
     CDS             complement(14814..15782)
                     /gene="ispH"
                     /locus_tag="WGO49_000830"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ0305961.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAASRGIDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(15811..16308)
                     /locus_tag="WGO49_000831"
     CDS             complement(15811..16308)
                     /locus_tag="WGO49_000831"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ0305962.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT         3798 a         4789 c         4307 g         3647 t
ORIGIN      
        1 ccccgtacag accgattttg acaacacctg cggcgcgcgc ccgattcttc ggaacacgcc
       61 ccccttcaga cggcataggg tcggaaatgc cgtctgaacc cgacggacaa accatcatgc
      121 tgaacacctc cctttccccg tggccctgct tcacccaaga agaagccgat gccgtttcca
      181 aagtcctgct gtccaacaaa gtcaactact ggacgggaaa cgaatgccgc gaatttgaaa
      241 aagaatttgc cgcctttgcc ggcacgcggt acgccgtcgc cctttccaac ggcacgctgg
      301 cactcgatgt cgcgctcaaa gcaatgggca taggcgcggg cgacgatgtg attgttactt
      361 cgcgcacctt cctcgcctcc gcctcctgca tcgtgaacgc aggcgcaaac cccgtgtttg
      421 ccgacgtgga tttgaacagc caaaacatca gcgcggaaac cgtcaaggct gtgctgaccc
      481 cgaataccaa agcgattatc gtggttcacc ttgccggtat gcctgccgaa atggacggca
      541 ttatggcttt ggcaaaagaa catgatttgt gggtgattga agactgcgcc caagcgcacg
      601 gcgcaaaata caaaggcaaa tccgtcggct ctatcggaca cgtcggcgcg tggtcgttct
      661 gccaagacaa aatcatgacc accggtggcg aaggcggtat ggttacgacc aacgacaaaa
      721 ccctgtggga aaaaatgtgg tcgtacaaag accacggcaa aagctacgat gccgtgtacc
      781 accgcgaaca cgcgcccggt ttccgctggc tgcacgaaag tttcggcaca aactggcgta
      841 tgatggaaat gcaggcggtc attggccgca tccagctcaa acgcctgccc gaatggacag
      901 cacgccgcca agaaaacgct gccaaactgg ctgaaagctt gggcaagttt gccagcatcc
      961 gcttggttga agtcgccgac tacatcgaac acgcgcaata caagttctac accttcgtca
     1021 aacccgaaca cctcaaagac ggctggatgc gcgaccgcat cgtgaacgaa ctgaacgcgc
     1081 gcaaagtccc ctgctatcaa ggcagctgct ccgaagtcta tttggaaaaa gccttcgaca
     1141 acacaccttg gcggccgaaa gagcgtttga aaaacgccgt cgaactgggc gacaccagcc
     1201 tgatgttctt agtacatccg acgctgaccg acggcgaaat cgcgttctgc aaagaacaca
     1261 tcgaagccgt attgaccgaa gccacacgat aacccttcag acggcatatg ccgcctgaaa
     1321 gcacataccg ccccacgata tgactctgga aaccctgctt gccctaccgc gcaacatcaa
     1381 gaaaatctgt ttcctcatac acgattttct gatgattttc attgcctttt ggtttaccca
     1441 aagcctgaag gccgactact cgaacgaatg gttcgaccct gccaactggc agtctttcct
     1501 attgaccgcg ccgttgacca tcggcctgtt cgtcagaatg gggctctacc gtgccgttac
     1561 acgcttcatc agcttccgca tcctctccac cgcgctggca ggcagcctcg cctccgccgt
     1621 attgttcttc ctcaacacat tgatttttga agaaaggctg cgcctcgccc tgccgattgt
     1681 ctatttctta ctgctgtttg tttccgtgac cggctcgcgt atggttttgc gcggactgtt
     1741 gtccgaacac cccaaaaaac agatgatccc tgtcatcatt tacggcgcgg gacggtcggg
     1801 cagacaactg cttgaggccg tcaaacaaat gcgcgaatat tccgccgccg cctttgtaga
     1861 cgacgacccc aaactgtggc acaccgtcat ctacgacctt gccgtttacc agcccgatgc
     1921 catcgccttc ctcatcgaac gctacggcgt ggaaaaaatc ctgctcgcca tccccggcgc
     1981 gacccaggaa caacgccgcc gaatcatcaa caaactggaa gcctatccgt gcgaagtgtt
     2041 gaccattccc ggaatgaaag acctgatgga cggaaaaatc agcatcggca cgctcaaaaa
     2101 aatctctgtg tccgacctgc tcgggcgtga ttccgtcgcg cccgacgacc gcctgatgag
     2161 tgccgacatc gaaggcaaaa ccgtcatggt aaccggcgcg ggcggctcca tcggttcgga
     2221 actctgccgc cagattatcc gccgccgccc cgaaaagctg ctgctgttcg agttatccga
     2281 attcgccctg tacgccatcg aaaaagaatt gcgcgaaacc tgcatccaaa aacgcctcga
     2341 caccgaaatc ctgccctttc tcggttcggt gcaaaaccgc acgctgctcg aacacgtcat
     2401 gaccgccttt tccgttgcga ccgtctatca cgccgctgcc tacaaacacg tccccatggt
     2461 cgagttcaac accgtcgaag gcatacgcaa caacatcttc ggcacactcg agtgcgcgct
     2521 tgccgccacg acatcgggcg taagaacttt cgtcctcatc tccaccgaca aagccgtccg
     2581 ccccaccaac accatgggtg ccagcaaacg catggcggaa ctctgccttc aggcactcgc
     2641 cgccgaaccc ggacaaaaaa cccgcttcag catggtacgt ttcggcaatg ttttaggttc
     2701 gtccggctcc gttgtcccgc tgtttgaaaa acagattgca gaaggcggcc cgcttaccct
     2761 gacccacccc gaaatcacac gttatttcat gaccataccc gaagccgccc aactcgtcat
     2821 acaggcaggc gcgatgggta cgggcggcga cgtattcgtc ctcgacatgg gtgaatccgt
     2881 caaaatcatc gaccttgccc gccaaatgat taccctaagc ggcctcaaac ccaacacacc
     2941 cgaacaaccc gacggcgaca tcgaaatcct cattaccgga ctgcgtcccg gcgaaaaact
     3001 ctacgaagag ctgctcatcg gcgacaacgt ccgcaaaacc gcccatccgc gcattatgac
     3061 ggctgacgag gcgatgctgc cgtggcacga gctctctgtc ctgctcgacc gcatccgttc
     3121 ggcctgcgac cgttacgacc agcaggcaat ccgcaccctg ctcatcaacg ccccgaccgg
     3181 ctttgccccg agcgacggca tctgcgacct gctttgggta cgagaaacac acagaaaaaa
     3241 tgccgtctga accttcagac ggcataacgt acaaaccaac ctaccttaca cacacggagt
     3301 ttgacatgca gttctcagca ttcggcgaaa aattcacgca acacagcggc atcctccaac
     3361 tgatggacga cctcggcgac gcgctcaaaa gcgacaagcc cgtcaatatg ctcggcggcg
     3421 gcaatccggc gcgcattccg gaaatcgatc gggcgttcgc cgacatattc tccaaactgg
     3481 cggcggaaca cgctgtcgag aacatcggca actactccaa tccccaaggc gatgccgcgc
     3541 tgattgacgc gctgaccgcc ttcctcaacc gcgaatacgg ctggaacctg accgccgaca
     3601 atatcgcgct gaccaacggt tcgcaaaacg cgtttttcta tttattcaac ctcttcggcg
     3661 gcaaattcaa cctttcagac ggcacatccg cagaaaaagc cattttgttg ccgctcgcgc
     3721 ccgaatacat cggctatgcc gacgtgcatg tcgaagggca gcacttcgtt tccgtcaagc
     3781 ccaaaatcga aaacgtcgaa cacgaaggcg aagcaggctt cttcaaatac cgcgtggact
     3841 ttgacgcact ggaaaacctg cccgaactca aagcgggcaa aatcggcgcg atttgctgtt
     3901 cgcgcccgac caacccgacc ggcaacgtgc tgaccgacag cgaaatggcg cggctggacg
     3961 ctttggcgcg tgaacacggg attccgctga ttatcgacaa cgcctacgga atgccgttcc
     4021 ccaacatcat ttacagcgac gtaacgctga attggcacga aaacatcatc ctctgcttca
     4081 gcctttccaa agtcggcctg ccgggcgttc gcaccggcat catcgtcgcc gcgcccgaag
     4141 tcgtcaaagc cgtcagcagc ctgaacgcga ttgtgaacct tgcccccacg cgcttcggcg
     4201 cggccatcgc cgccccgctg ctggaaagcg gcgagatgaa acggcttgcc gaccaagtca
     4261 tccggccgtt ttaccgcaat caggcgcaaa ccgccgtctc gctgctcaag cgcgagctgg
     4321 gcgcgtaccc gatgaaaatc cacaaacccg aaggcgcgat tttcctgtgg ctctggtttg
     4381 aaaacctgcc cgtttcttcg caaaccctgt acgaaatgct caaagccgaa ggtacgctga
     4441 ttattccggg cgagcatttc ttcgtcggca tcgacacgcg ggattacccg cacgcgggcg
     4501 agtgcatccg catgagcatc gcgcaggacg ctcaaacgct ggaaaaaggc atcgccgcca
     4561 tcggcaaaac cgtccgaaaa ctgtacgaca acgtttaaac cgcaaaaaat gccgtctgaa
     4621 aggttttcag acggcatttt tatctgcatt caatatcggg aaaaatgcgc ccacaccggc
     4681 ctgcagtttt ccggcagttc gggacacgcg ccgagaatgc cgccgctaaa gtcgtttaaa
     4741 cggtggaagt cgctgcccgc gctggcgagc ataccgaagc gttctgccaa aagcgcgtag
     4801 ttgaggcggt cgtttttgca gcagttgccg ctgtggactt cgatgcctgc gccgccgagg
     4861 tttttaaatt cttcaaacag attgcgcttg gcggtggcgg acaaatcata acgcatcgga
     4921 tgggcgatga ccgccaaccc gcccgcgccg ttgacggcgg agacgcagtc ttccagcgtc
     4981 gcccattcgt ggcggacggc gcaggatttg ccgtcgccca agtatttggt gaacgcctgc
     5041 tgcttgtttt tgacgtgtcc cacccgaatg aggaactcgg cgacgtgtgt gcggctgacc
     5101 atttctttgt ttgccgccag cgcgagcgcg ccgtcgtatg cgcccgaaat gccgcgtttt
     5161 tccagcttgg cggcgatggc ttcgaggcgt ttcagacggc cttgccgcac ttgcgccaac
     5221 aggttttgca ggttttcgtc ttgttcgtca aagtctaagc cgacaacgtg tatggtacgc
     5281 ccgcgccacg ttacggagat ttccacgccg ttaatcaggc gcaaaccgag cctgtcggct
     5341 tcggcacgcg cttcggcgat gccgccggtg tggtcgtggt cggtcaacgc cagcagcgtg
     5401 cagccgtttt gatgcgcgag gcgcacgact tcggcggggg aaagcatacc gtcggaaacg
     5461 atggaatggc agtgcaggtc tatcatgggg tgttatgtgt gtggtgaatg aaggtcggga
     5521 tgtttcaatg ttgttgggca gtgtggcggc ggcggtcatt ttaaaaaaca ggtgtatggc
     5581 aaatgcaaag gaaaagccgc tatgccgtca ttcccaaccc ccaaatgtac ggatagagcc
     5641 agtagggatg ggtacattat ttgtttgcat tattagttat cctgctgtat ccattaaatc
     5701 atatgcttca attaattgtt gttctagctc ttgtaccaat tttggattgc gaattcctaa
     5761 cgcaatttca aacagcgagt tctcactatg tacatctaca cagtatctac ataaagatct
     5821 tgtaaagata taggttcttg gctgatggca ctcattattc caatagttaa tttgcataca
     5881 tatttgttta ataaaatttt atctgtatca aaatgataaa cttgttgagt tagtatccaa
     5941 gggtattctt ggtatatttt ctgtgataat ttttcattct ctataatcgc gaaaggtatc
     6001 tgtttacata acataccaac agacttttcg tttagacaga aaatagccca attttcccca
     6061 tattcccaag agtatgtaaa atctgtttcc tgaacaatat cttgtaaaac ttgctcaaaa
     6121 tccatgtttt gccatttacc tttatacata cctaatgcgg cactggttac tatttcgttt
     6181 aagtcataaa tagccattta tgttccttgc taatttctag gtgagacatg tataggactg
     6241 agaccagtat gcccattagg ttcccctcta tacacattaa tagtagatgt tggttccgtt
     6301 ttcagacggc atcgtccgac caacccgctt ctttcaattt ccgccgttgc gcgtcgtagc
     6361 gggccttatc cgcccaataa atcgtctgaa tgcacgcctc gccgcagtcg ctgccgcaac
     6421 attcccacga ctcggggcgg ataggttcgt ctaaaagcgg ttcgcccaag atggcttcgg
     6481 ctttattctt cagggtcgta tccatcggca atttccaagc gtgcgccgtc aaattcaatc
     6541 acttcgccgc cgcgtatttt ggcggtttta cgggtttcgg tttcgccgtt gcgcaacacc
     6601 agcccttcgg cgataaacgc ttttgcctgt ccgccgcttt cagcaagacc ggccagtttc
     6661 aacaggtcgc ataaagcgat gtattcatta tcttcaagat agactgttgc ttccatcatt
     6721 caatcctttt caataagggc gatattttaa catggccgtc tgaaagaaag cccccttctg
     6781 tttgatttgc tttatccaac cccattacat ttgattttca gacggccttt agctgccaac
     6841 cctgctacaa tacgcctttt attgttcacg ccgattttgc catgaccgag ccgacctaca
     6901 tccccctgcg cctgcatacc gaattttcga ttaccgacgg tatggtgcgg attaaaaaac
     6961 tgattgccaa agcgcaggaa tacggtttgc ctgctttggg catcagcgat ttgatgaacg
     7021 aattcggttt ggtgaaattt tataaagcct gccgcagcgc ggggattaag cccgtcggcg
     7081 cggcggacgt atggataggc aatccggatg cgcccgacaa gccgttccgt gctatgctga
     7141 ttatccgcaa cgatgcgggc tatctgcgct tgagcgagct tttgacggcg gcttatgtcg
     7201 gcaaagaccg caatgtccat catgcggaac tcaatcccga atggctggaa aacggcgaca
     7261 acagcggctt gatttgtttg agcggcgcgc attacggcga agtgggcgtg aatctcttga
     7321 acggcaacga agacgcggcg cgggcggcgg cgttgaaata tgcggcgtgg tttcccgatg
     7381 cgttttatct ggagttgcaa cgcctgcccg agcgtcccga atgggaggct tgcgtttcgg
     7441 gcagcgtgaa gctggcggag gaactgggtt tgccggtggt ggcgacgcat ccgacgcagt
     7501 ttatgagccg cgacgatttc aacgcgcacg aggcgcgggt gtgtatcgca ggcggctggg
     7561 tattgacgga caagaaacgt ccgcgcgatt tcacaccgag ccagtttttc attccgccgg
     7621 aaacgatggc cgaacgtttc gccgatttac ccgaagccct cgaaaacacg gtggaaattg
     7681 ccaaacgctg caatctgcac atcacattgg gcaaaaactt cctgcccctt ttccccacgc
     7741 ccgacggttt atcactcgat gactatctca tcaaactctc caatgagggc ttgcaggaac
     7801 gcatggttca gctttatccc gacgaggcgg agcgtgcgga aaaaatgccg gaatatcagg
     7861 aacgtttgga ttttgagctg aacatcatca tccagatgaa attccccggc tatttcctta
     7921 tcgtacaaga ctttatcaac tgggcgaaaa cacacggctg cccggtcggg ccgggacgcg
     7981 gttcgggcgc gggttcgctg gtggcgtatt cgctgaagat taccgacctc gatccgctga
     8041 aatacgcgct gctgttcgag cgtttcctaa accccgaacg cgtttctatg cccgacttcg
     8101 acgtggactt ctgtcaggca aaccgcggcc gcgtgattga atatgtacgc gaaaaatacg
     8161 gtgcggaagc agtcagccag attgttacct tcggcacgat gtcgtccaaa gcagtgattc
     8221 gcgacgtggg gcgcgtactg gaactgccgt ttatgctgtg cgacaaactg tccaagctga
     8281 ttccgttgga agccaacaaa cctttgggtt tggacgacgc gatgaaggcg gagccgcaga
     8341 ttcaggaatt aatcgaagcg gaagaagcgg acgaactgat tacgctggcg aaaaagctgg
     8401 aagatttgac gcgcggtttg ggtatgcacg cgggcggcgt gttgattgcg ccgggcaaga
     8461 tttccgatta cagccccgtg tatcaggcgg acgaatccgc ctcgcccgta tccatgtacg
     8521 acaagggcga cgtggaagac gtgggtttgg tgaaattcga ctttttaggt ctgcgcaacc
     8581 tgaccatcat cgaaatggcg cagaacaaca tcaaaaacac caccggcgac atcatcgatg
     8641 tcggcaaaat tccgcttgac gaccaggttg cctaccaaat cttccgcgat gcgaacacca
     8701 ccgccgtgtt ccagttcgag tcgacaggca tgaagaaaat gctgaaaacg gcgcatacga
     8761 ccaagtttga agaactgatt gccttcgtat cgctctaccg acccggcccg atggacaata
     8821 ttcccgactt cgtcgcgcgt atgaaaggtc aggaattcca atacatccac ccgctactcg
     8881 aaggcatcct cgcaccaacc tacgggatta tggtgtatca ggaacaagtg atgcaggcgg
     8941 cgcagattat cggcggctac tcgctcggcg gcgcggacct gctgcgccgc gccatgggta
     9001 agaaaaagcc cgaagaaatg gtgaaacacc gcgaaatctt cgccgaaggc gcggcaaaac
     9061 aaggcatttc gcgcgaaaaa tccgacgaaa tcttcaacta catggaaaaa ttcgccggct
     9121 acggtttcaa caaatcccac gctgccgcct acgccctgat ttcctaccag accgcatggc
     9181 tcaaagccca ctaccccgcc gaatttatgg cggcgaccat gtcgtctgaa ttggacaaca
     9241 ccgaccagct caagcatttc tacgacgact gccgtgccaa cggcatcgag ttcctgccgc
     9301 ccgacatcaa cgaatcagac taccgcttca cgccgtatcc gaacatgaaa atccgctacg
     9361 cgctcggcgc gattaaaggc acgggcgaag ccgccgtcga atccatcacc gccgcgcggc
     9421 aaagcggcgg caagtttacc ggcctcttgg acttctgtga gcgcgtcggc aaagaacaca
     9481 tgaaccgccg caccctcgaa gctctgatac gcggcggcgc attcgacagc atcgaaccca
     9541 accgcgccat gctcttggcg aacatcgacc tcgctatgga caacgccgac caaaaagccg
     9601 ccaacgccaa tcagggcggg cttttcgaca tgatggaaga cgccatcgaa ccggtgcggc
     9661 tcatcgacgc gccgatgtgg agcgaatcgg aaaaactcgc cgaagaaaaa accgtcatcg
     9721 gcttttacct gtccggccac ccgttcggcc cgtatgccca agaagtccgc caaatcgccc
     9781 cgcagaaact gtcgaaactc aaaccgcaag acagcgtgcg cctcgccgga ttcgttaccg
     9841 ccgtacgcac catgatgggc aagcgcggca aaatcgcctt cgtcagcctt gaagatttga
     9901 gcggacaggt tgaaatcatg gtcggcggtc agacgttgga aaactgcgcc gactgcctca
     9961 aagccgacca agtgctgatt atcgaatcca aagtcagccg cgacgactac ggcggcggcg
    10021 acggactgcg tattctggca aaccaagtca tgaccctgca aacggcacgc gaacgctacg
    10081 cccgcagcct cagcctcgcc ctcgccccgc atcacgacat cggcggactg gtacaactgc
    10141 tcgccgccca ccaactgccc gacacgccgc gcatcccgct gcaactgtcg tatgccaacg
    10201 aaaaagcgtc gggcaggctt caagtgccgc cgaaatggac ggttacaccg agctccgcat
    10261 tgttcggcga actggaaaca ttgctcggca gccggtcggt gcgcgtcaac tggtaaccaa
    10321 aaatagaaat gccgtctgaa gcccaaaaac cggtttcatt cgtactttat tcgaatgatt
    10381 gaataaaagt aactgccaag aaaaacgtat tttttggtta tttcgccagt ctaaatagag
    10441 caaccgggac gattgatatc cgtgtgcatg acacagacag caccaaaggg aaaaacggca
    10501 ttttccaaag tatcggtatc aaaaccgccc tttcactcca aaaataccaa atcgacaaac
    10561 cgggcaaaga aatcagaccg tgccgcctga aaaaacgccc acccgtccgt taaattaacc
    10621 tatccctgtt tcagacggcc tgaagcaggg atttttatat caaaataaaa tgagaaaggg
    10681 agcaataacc cttaggtagc tcttgttatt ttccgatgca aaacaaagca gtcatatatt
    10741 taattccccc tacctctgcc aagccttcct caaatattcg acgcaatcgg tcagcgagta
    10801 gaacgggaca ttgccgtggt cggcattcgg atattcccgg aaaaagacgg cggccccgtg
    10861 cctgtctaac tctgccgcca tttgttcggc ctgccctgcc atatcgcgtt cttccctgcg
    10921 tttacaatcg ctaccccgtt ctagcgcgcc gatgttgagg cagacatcga tgccgtttag
    10981 ccggttttca gacggcataa agtcgagtat ccgcctgttg tgccaccaaa tcgatgggga
    11041 tacgagccaa tgccgtctga aacggcggtg ggaaagcagg gaatacagtc cgaacagtgc
    11101 gccgaacgag tgtccgaata cggcggtttc attgcggttg agggtgtagc ggctttctaa
    11161 aaaggcggtc agctcgctgt cgataaaggc ggcgaagcgg tctgcctgtc cgaactgctg
    11221 ccgttcgtct gctgtggcgt tgtctccaag cggcggcgtg tagtcggcgg cacgttgtgc
    11281 caaatcgcgc acactgcctg tcgtgtagcc gataccgaca atcaggcagg gggcgttgct
    11341 tcgggtaacg gggttgggca tcagcgactg catgatgttg aaaagtgcgg ggaaaaaggc
    11401 ttcgccgtcc aaaacgaaga ggacgggata gccttcagac ggcatttcgc cgagtgtcgc
    11461 cgtctgaatc cgatagattc gccccgtgca ggtggatttg atttcggttt caaaggcttg
    11521 gggcagtatg gcaggttgga atgtgtcggt cggtatgggt ttcatgatgt tcggcttgtg
    11581 ggtcagactg gcggcaagcg gtaaaaccga accggcaaag cggtctgcct gccggttcgg
    11641 tctgttttcc ttcgcaatgc catactccag ttgtgagagc atagggtatg cagcgcagct
    11701 tgttgtggtt tgatgatgct gcggctgccg tttcagacgg catattggtc tttaaaaact
    11761 gtaacgcagg tttgccgtca ggctgcgctc cgaaccggga atgttaaagg tgttcgcgcc
    11821 gccgacgcgg gcgtagtaat ggcggttgaa gatgttgtcg gcgttgattt gcagcttcag
    11881 cttgggcgtg aagcggtatg ccgccatcgc atcgaacgtg gcataaccgc ctgcatgcat
    11941 ccctgcagat gaagtaatgc cgctcatcgc gttcacgccg ccgccgatgg tcagctcggg
    12001 cgtaacttgg taagtcgtcc acaggtttgc gctgtgtttg ggcatcaata ggaagatgcc
    12061 gtcgtcgcgt gaattggagg cggttttgat ttggctgtgc agatagctgt aacctgcatg
    12121 gatttgccat ttcggtgtaa ccgcgccgct gatttcggtc tcaacgcctt ccatcacgcg
    12181 tttgcccaat gcggcgtaac gggttttttt gttgttcggg ttcaacggtg cggcggcgtt
    12241 tttgtctttc atgcggtaga acgaaactcg ggcattgagg cggtcgtcca tgtagctgcc
    12301 tttgtaaccg acttcaaact ggttgccttg gcgcggtttg agcagcttgc cgtcggcatc
    12361 gaggttggtt tgcggtgtgt agagctggga caggctcagg tagaggctgt tgttgtcgtt
    12421 caagtcgtaa accgcgcctg cgtagccggt gaacttggtt ttgctggctt tgtgcagggt
    12481 tttgccttcg cccaactcga ttttatagtg tcccaaacgt ccgccgccga taagcgacag
    12541 cccgtcggca ggatggaagg tggatttgcc gtaaatgccg aattcgtcga ggttttcggt
    12601 agcgacggta tggctgtaac cgcgcacgcc tttgcgcgcg ttggcaatca aatcaacctg
    12661 cggtatgctg cggaactcgt ttaaagccag gccgccgcgt gcatataaag tagtacggcc
    12721 ttgttcgttg gtgctgcgga agcggttgta atcggcgccg atgacaaatt cgttggccgt
    12781 attgcccaag cggaaaggcc tgctgtagct ggcgtcaaac gcgagggctt tttgtttgat
    12841 ttctgtaccc aaacccaccg cgcaggcttt gtcgtcagcc gtattgcagc ccgggcggcc
    12901 tgccagggtt ttcatgccca gcttgctgcc ggcaaaggca tagttggagt cggcatcgcg
    12961 gtcggaatag cgcataccga ctttgccgta gccgccgttg ccgaagtaat gtttcaaatc
    13021 ggcaaacacg tcgtggctgt tcattttaaa tttgttccaa tccgcgccga caaatacgtg
    13081 ttgcggcagg gacggtaatt tgttattggc atcggctggc aagccgttgt acggcgcgag
    13141 gtggcgttgc tggtaaagat agcccgcgcc caaaaccgta tcggggttga tgtcccaatc
    13201 cgccgccgcg tagaaggttt cgtgccggtt gtttttctcg gcgggacgcg gagacgcgcc
    13261 gacggtctgc gccatcacgc ggccgcgcac gctgccgtct gaattgaggc tgcccgatac
    13321 gtccgcctcg gctttatatt gtttgtgcgt accgaaccct gccgcagcat gaccttggaa
    13381 cgctttggcc gggcgtttgc acaccagatt tacgatgccg cccatctcgc cgctgctgtc
    13441 gaacaagccg ctcggcccgc gcatcacttc cacacggtcg aaggcaaaca gattgggcag
    13501 cgtgccgttg atactctgca tctgcgcggg caggccgtcg atgttgtatt cgctgtattc
    13561 gtaaccgcgc gcgtaaaccg aagagcgtcc gtcgtcgttg ctcaacacgc gcaggccggg
    13621 cgttttgcgt gccaactggt caaacgtatc aacattgcgg tctttgacct gttgattggt
    13681 gatgatgctg acagattgcg gaatttcgcg caaagaagcg gggatttttg tgccgacggt
    13741 ggcggcaaac gagctgtaat cgccgttttt ctcggtggca atcgcgttgt acgaacgctg
    13801 acccttaata tggacggttt ccaaatccgc cgtttgtgcg gcaaaaaccg aagacgaaag
    13861 tgctgccaaa accgtggcgg cggtcatatt gatgcggaaa actgacataa actgtcccat
    13921 tcacataaat gataatggtt ctattttaat aaagcacaac gcggcttgtt cggaaaaaca
    13981 tatcgcgcag ccgacaaatt ttgtcgaaaa tgcgacacgt ctgcgttttc cgcataaaat
    14041 ctgacttttc accgtaaaca acctgccatg accacgccca aactcatcat cttcgactgg
    14101 gacggcacgc ttgccgatac gacccaaccc atcatcgaca ccatgcgccg cagcttcgcc
    14161 gaatgcggtt ttccgccgcc cgaagcggaa cgcgtccgca gcctgattgg ctacagcctg
    14221 cccgaaatca tccgcgacct gctcgaaatg ccgtctgaaa ccgccgttgc cgacatcgcg
    14281 cgcacttatt ccgcgcatta cctcaatccc aacaaccgca atatgacgct ctttcccgat
    14341 gccctgccct gtctggacaa gctcaaagca caaggcttcc ggcttgccgt cgccacgggc
    14401 aaagggcggg cgggtttgga caacgccatc agccaaaccg ccaccggcgg ctattggctc
    14461 gccaccgcct gcgcggggga atatccctcc aaaccctcgc ccgaaatggt attcggaatc
    14521 tgcggcgaac tggggttcga cccgaaagag gcattggtcg tcggcgatac ggcgcatgac
    14581 ctgcatatgg cggcaaacgc aggcgcggcg gcagtcggcg tggccaccgg cgcacattcg
    14641 cgcgaacagc tccttagcgc accgcatctc gccgtattgg acggtttgtc cgaactgccc
    14701 ggttttcttg cacgacatta cgcctgattg gtttccgcat ccggcacgcg gcaaaaatgc
    14761 cgtctgaagc ctgttcagac ggcatttgtg ttgcccaaac attcaacgct tgctcaacgt
    14821 ttgcacaaat cgggtttggt ttcgccctcg cggcgcagct ctttgggcag gacgaacaca
    14881 atgctttctt ccgcaccctc gccttcgcgt acggtttcgt gcccccatcc gcgtatggtt
    14941 gccagtactt cccgcaccaa cacttcgggc gcggacgcgc ctgccgttac gccgactttg
    15001 tttttgccct caaaccatgc gcgttgcagg tagcctgcat tatccaccat atacgcatcg
    15061 attccgcgcg atgccgccac ttcgcgcaag cggttgctgt tggacgaatt gggcgaaccg
    15121 accacaatca cgatgtcgca ctgttccgcc agctctttga cggcggtttg ccggttggtc
    15181 gtcgcatagc agatgtcttc cttgtgcgga ttgcggatat tggggaaacg cgcgttcagc
    15241 gcggcgatga tgtctttggt ttcatcgacc gagagcgtgg tttggctgac ataggcgagt
    15301 ttgtcggggt ttctgacttc gagttttgcc acatctccga ccgtttcgac caaaagcatt
    15361 ttgcccggcg caagctgccc catcgttcct tcaacctcga cgtgcccctt atgcccgatc
    15421 atgatgattt cacagtcttg ggcatccagt cgggcgactt ccttatgcac tttcgtcacc
    15481 agcgggcaag tcgcatcaaa cacgcggaaa ccgcgctccg ccgcttcttg ccgcaccgcc
    15541 ttcgatacgc cgtgtgccga ataaaccagt gtcgcgcccg gcggcacttc cgccaagtct
    15601 tcaataaaca ccgcaccttt ttcacgcagg ttgtccacga cgaatttgtt gtgaacgact
    15661 tcgtggcgca cataaatcgg cgcgccgaac tcttccaaag cacgttcgac aatactgatt
    15721 gcccgatcca caccagcgca gaagccgcgc ggattggcaa ggatgatggt tttctcgttc
    15781 ataagcccgg tatttcgttt tcagacggca tcaatatttt tcttcttggg ttttacggtg
    15841 gacgatgttg tccaacaccg ccaacaccgc accgacgcag ataaagctgt cggcaatatt
    15901 aaaggcggga taaaaccaat tttgccaata aaacaataag aaatcgacga catgaccgtg
    15961 tatcaggcgg tcgatgacat tgcctaacgc accgccgata atcattgccg cacccgtttt
    16021 gccgagggtt gcaaactcat cgcgcaagat ggcgcgtacc aaatacgcgc tcaccgccac
    16081 cgccagcacc aaaaaaaagt atttttgcca gccgccctga tcggcaagga agctgaacgc
    16141 cgcacccggg ttgtacacca gcgtcagatc gaaaaaggaa ggaatgacat tgacgcgttc
    16201 ccgatactga aacgacgaca gcaccgccca cttcgaccac tggtccagca cgatggcggc
    16261 aagtgccaat acccaatagc gcgttttact tgaaacagat gaagacatat ttttcaacag
    16321 ccggtaaaag agtaccattt tacccgaaaa ccccctttcc tgtacccgaa acggcaaatg
    16381 ccgtaatctt aaaacccgtc attcccgaca acaccgtaat ctcgaaaccc gtcattcccg
    16441 cgtaggcggg aatccagacc tgtccgcaca gaaacttatc ggataaaaac agttgcccaa
    16501 accccgcgtt ctatagtgga ttaaattcaa accagtacgg c
//
LOCUS       DAWFAD010000035        16473 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWFAD010000035 DAWFAD010000000
VERSION     DAWFAD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16473
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.035"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            241..1305
                     /locus_tag="WGO49_000832"
     CDS             241..1305
                     /locus_tag="WGO49_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ0305963.1"
                     /translation="MKAARFYDKGDIRIKDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGSGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKEGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVNPNL"
     gene            1446..1769
                     /locus_tag="WGO49_000833"
     CDS             1446..1769
                     /locus_tag="WGO49_000833"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ0305964.1"
                     /translation="MGDSVLSTIQQTIDRRKSADPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMSSKDKDPSHLVYEVADLWFHTMILLAHHNLKAEDVLDELARRQGLSGLVEKA
                     ARTES"
     gene            1842..2165
                     /locus_tag="WGO49_000834"
     CDS             1842..2165
                     /locus_tag="WGO49_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ0305965.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2212..2415
                     /gene="tatA"
                     /locus_tag="WGO49_000835"
     CDS             2212..2415
                     /gene="tatA"
                     /locus_tag="WGO49_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ0305966.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2419..3105
                     /gene="tatB"
                     /locus_tag="WGO49_000836"
     CDS             2419..3105
                     /gene="tatB"
                     /locus_tag="WGO49_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ0305967.1"
                     /translation="MFDFGLGELVFIGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKETGTDMEGNLHDISDGLKPWEKLP
                     EQRTPADFGVDENGNPFPDAANTLSDGISDVMPSERSDTSAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRGLRPKHR
                     AKPKLRVRKS"
     gene            3119..3889
                     /gene="tatC"
                     /locus_tag="WGO49_000837"
     CDS             3119..3889
                     /gene="tatC"
                     /locus_tag="WGO49_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ0305968.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3942..4550
                     /gene="maf"
                     /locus_tag="WGO49_000838"
     CDS             3942..4550
                     /gene="maf"
                     /locus_tag="WGO49_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ0305969.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPANIDETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            4559..4912
                     /locus_tag="WGO49_000839"
     CDS             4559..4912
                     /locus_tag="WGO49_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ0305970.1"
                     /translation="MNRQTAYFLASFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4902..6716
                     /locus_tag="WGO49_000840"
     CDS             4902..6716
                     /locus_tag="WGO49_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ0305971.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALRLQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPPISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACKSLVAHYGQERIVTLLAWSLLIGSLLQSCIVVIQFAGWEDTPM
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIYDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPAPDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6906..7583
                     /locus_tag="WGO49_000841"
     CDS             6906..7583
                     /locus_tag="WGO49_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0305972.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7596..9002
                     /locus_tag="WGO49_000842"
     CDS             7596..9002
                     /locus_tag="WGO49_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0305973.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9120..11546
                     /locus_tag="WGO49_000843"
     CDS             9120..11546
                     /locus_tag="WGO49_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ0305974.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EENQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQSAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHSLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11591..11836
                     /gene="rpsP"
                     /locus_tag="WGO49_000844"
     CDS             11591..11836
                     /gene="rpsP"
                     /locus_tag="WGO49_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ0305975.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11852..12361
                     /gene="rimM"
                     /locus_tag="WGO49_000845"
     CDS             11852..12361
                     /gene="rimM"
                     /locus_tag="WGO49_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ0305976.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTVSVTLEAGKVVNSELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12361..13110
                     /gene="trmD"
                     /locus_tag="WGO49_000846"
     CDS             12361..13110
                     /gene="trmD"
                     /locus_tag="WGO49_000846"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ0305977.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHQKAVEL
                     AELPHLILLCGRYEGIDERLLQSSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRILNKILQEQREIQS"
     gene            13126..13491
                     /gene="rplS"
                     /locus_tag="WGO49_000847"
     CDS             13126..13491
                     /gene="rplS"
                     /locus_tag="WGO49_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ0305978.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13813..14538
                     /locus_tag="WGO49_000848"
     CDS             13813..14538
                     /locus_tag="WGO49_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ0305979.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLTRQNIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFR
                     GINAVQKQRVHESLERVGMRQFAGRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPNTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPEWCAS"
     gene            14562..15437
                     /locus_tag="WGO49_000849"
     CDS             14562..15437
                     /locus_tag="WGO49_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ0305980.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALVSVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15514..16428
                     /locus_tag="WGO49_000850"
     CDS             15514..16428
                     /locus_tag="WGO49_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ0305981.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSGDIKKIRSAKLILLNGLGLEAADIQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALTNAMKQ"
BASE COUNT         4283 a         5289 c         3623 g         3278 t
ORIGIN      
        1 ccgtactggt ttttgttaat ccaccataaa tgccatctga agccgattcg gttttcagac
       61 ggcatcatta tttgacctga cgcaaacaga aagccttccg cgtgcttaaa cacatctctt
      121 gtagtaaaat atctaaacag attcaactgg aaacggtcag gcttcttcac gccattccca
      181 tttcaaccgc cgcctgccag attcagacgg tattcctact tgaaaacaag gagcgttaca
      241 atgaaagcag cacgttttta cgacaaaggc gacatccgca tcaaagacat ccccgaaccg
      301 accgtcgccc ccggcaccgt cggcatcaat gttgcctggt gcggcatctg cggtaccgac
      361 ctgcacgaat tcatggaagg cccgattttc attccgcctt gcggtcatcc gcacccgatt
      421 tccggcgagt ccgcacccgt cacgatggga cacgagttct ccggcgtggt ctatgccgtc
      481 ggcgaaggcg tggacgacat caaagtcggt caacacgtcg tggtcgagcc ttacatcgta
      541 cacgacgacg taccgaccgg ctcgggcagc aactaccacc tctccaaaga catgaacttt
      601 atcggcttgg gcggctgcgg cggcggcctg tccgaaaaaa tcgccgtcaa acgccgttgg
      661 gtgcatccga tttccgacaa aatcccgttg gatcaagccg ctttgatcga accgctgtct
      721 gtcggacacc acgcctatgt acgcagcggc gcgaaagaag gcgacgtcgc attggtcggc
      781 ggtgcaggtc cgatcggttt gctgttggct gccgtgttga aagccaaagg catcaaagtc
      841 atcatcaccg agttgagcaa agcacgcaaa gacaaagcgc gcgaatccgg cgttgccgac
      901 tacatcctcg acccgtccga agtcgatgtt gtcgaagagg tgaaaaaact gaccaacggc
      961 gaaggcgtgg acgtggcatt cgaatgtacc agcgtgaaca aagtgttgga tactttggtc
     1021 gaagcctgca aacctgccgc caatttggtt atcgtatcca tctggagcca ccctgcaacc
     1081 gtcaacgtcc acagcgtcgt gatgaaagag ctggatgtgc gcggcacgat tgcctactgt
     1141 aacgaccacg ccgaaaccat caaactggtc gaagaaggca aaatcaacct cgaacccttc
     1201 atcacccagc gcatcaagct ggacgaactg gtttccgaag gcttcgagcg tctgatccac
     1261 aacaacgaat ccgccgttaa aattattgtg aatccaaacc tataagcaaa ttacatttgc
     1321 aacagcacaa atgccgtctg aaacgctcag acggcatttt ccaacgaaac gcaacaggcg
     1381 tattgcccga gagcagccgt ttaacctatc attccgaaca aacagaaaca aacagaagga
     1441 aaatcatggg agattccgta ctatccacca tccaacaaac catcgaccgg cgcaaatccg
     1501 ccgatccgtc cgaatcctac gtcgcacaac tcctgcacaa gggcgaggac aaaatcctaa
     1561 aaaaagtgat tgaagaggcg ggcgaagtat taatgtcatc caaagacaaa gacccgtccc
     1621 acctggttta cgaagtcgcc gacctttggt ttcacacgat gatcctgctg gcgcaccaca
     1681 acctgaaggc ggaagacgta ttggacgaac ttgcacgccg tcaggggctg tcggggctgg
     1741 tcgaaaaagc cgcccgcaca gaatcttaaa tttatattac aatctcatcc tttccacgtt
     1801 taatccgtct gggtgcttct tcagacggca acggagccgt tatggacaac tgtattttct
     1861 gcaaaatcgc cgccaaagac attccggcgc aaaccgtcta tgaagacggc gaaatggttt
     1921 gtttcaaaga catcaacccc gctgctccgg ttcatctgct gctgattccc aaagtccatt
     1981 tcgattcgtt ggcacacgcc gcgcccgaac atcagcccct tttgggaaaa atgatgctga
     2041 aagttcccga aatcgccaaa gcggcaggac tggcagacgg cttcaaaacc ctgatcaaca
     2101 ccggaaaagg cggcggacaa gaggtcttcc acctgcatat acacatcatg ggcacacccg
     2161 tataaaccgt tatttcacaa tcaaccccta atacttactt aaggatacat catgggcagt
     2221 ttttctctga cgcactggat tatcgtactg attatcgtcg ttttgatatt cggcaccaaa
     2281 aaactgcgca acgtcggcaa agacctcggc ggtgcggttc atgacttcaa acaggggctg
     2341 aacgaaggta cagacggcaa agaagcccaa aaagacgatg taatcgaaca caaaaaagac
     2401 gaagacaaag cgtaatttat gtttgatttc ggtttgggcg agctggtttt tatcggcatt
     2461 atcgccctga ttgtcctcgg ccccgaacgc ctgcccgagg ccgcccgcac cgccggacgg
     2521 ctcatcggca ggctgcaacg ctttgtcggc agcgtcaaac aggaatttga cacgcaaatc
     2581 gaactggaag aactaaggaa ggcaaagcag gaatttgaag ctgccgctgc tcaggttcga
     2641 gacagcctca aagaaaccgg tacggatatg gagggtaatc tgcacgacat ttccgacggt
     2701 ctgaagcctt gggaaaaact gcccgaacag cgcacgcctg ctgatttcgg tgtcgatgaa
     2761 aacggcaatc cctttcccga tgcggcaaac accctatcag acggcatttc cgacgttatg
     2821 ccgtccgaac gttccgatac ttccgccgaa acccttgggg acagcgggca aaccggcagt
     2881 acagccgaac ccgcggaaac cgaccaagac cgcgcatggc gggaatacct gactgcttct
     2941 gccgccgcac ccgtcgtaca gaccgtcgaa gtcagctata tcgatactgc tgttgaaacg
     3001 cctgttccgc acaccacttc cctgcgcaaa caggcaataa gccgcaaacg cggtttgcgt
     3061 ccgaaacacc gcgccaaacc taaattgcgc gtccgtaaat cataaagaga gcaatccggt
     3121 gtccgaaaca caaaacgaac aacccgtcca accgcttgtc gagcatctca tcgagctgcg
     3181 ccgccgcctg atgtggacgg ttgtcggcat cttagtctgc tttttcggcc taatgccgtt
     3241 tgcccaacaa ctctatactt ttatcgccga cccgctgatg gcaaacctgc ccaaagacac
     3301 cagcatgatt gccaccgatg tcatcgcacc gtttttcgtg ccggtcaaag ttaccctgat
     3361 ggcggcattt ttaatttcgc tgccgcatac gctctaccaa atctgggcat ttgtcgcgcc
     3421 cgcactctac caaaacgaaa aacgcctgat tacgccgctc gtcctctcca gcgtcagcct
     3481 gtttttcatc ggcatggcat ttgcctactt tttggttttc cccgtcattt tcaaattcct
     3541 tgccagcgtt acccctgtcg gtgtcaatat ggcgacagac atcgacaaat acctctcctt
     3601 catcttgggg atgtttgtcg cattcggtac aacgtttgaa gtccccattg tcgttatcct
     3661 gttaaccaaa attggtgtgg taacaaccga acagctcaaa cgcgcccgcc cctatgtgat
     3721 tgtcggcgcg tttgtcattg ccgccatcat cacgccgccc gatgtgattt cacaaaccct
     3781 gcttgccatt ccgctgattc tcttatacga agcaggtatt tggttcggac gctttttcac
     3841 gccacgttca gaacaggatg gcgacataca gccgcctgca acaacctgac actatgccgt
     3901 ccgaacctcc gcctcatacc gcaacagatt aaggaatacc tttgaatacc ctctatttag
     3961 gttcaaacag cccgcgccga atggaaatcc tgacacagtt gggctatcag gtcgtcaagc
     4021 tgcctgccaa catcgacgaa acggtcagac agaacgaaga ccctgcccgt tacgttcaaa
     4081 ggatggcaga agaaaaaaac cgaaccgccc tgaccctctt ttgcgaaacc aacggcacaa
     4141 tgcccgattt ccccctgatt accgccgaca cctgcgtcgt ttcagacggc attatattag
     4201 gcaaaccccg ctcccaagcc gaagcaatcg aatttttaaa ccgattgtcc ggcaaacaac
     4261 ataccgtcct gactgctgtc tgcattcatt atcgcggcaa aacgtcaagc cgcgtccaaa
     4321 ccaaccgcgt tgttttcaag cccctgagtt cggaagaaat ttccgcctat gtgcaaagcg
     4381 gcgaaccgat ggacaaagcc ggcgcctacg ccgtacaagg cataggcggc atctttatcc
     4441 aatctatcga aggcagcttc agcggcatta tgggactgcc cgtttatgaa accgtttcga
     4501 tgttgcagga tttgggatac cgctccccct tgtccgccct taaaccgtaa agaccgccgt
     4561 gaacagacaa accgcttact tccttgcctc cttcagcctg atcgcactga taatcctgtc
     4621 cctttcttgg gaactgtgga ttgcaccgtt acgcccgggc ggctcgtggc tcgcgctcaa
     4681 agccctcccc ctctgtctgc cgctttcagg catcttgaaa aagaaaatct atacctacca
     4741 atacagctcc atgctggttc tgatttattt tgccgaagcc gtcatgcgcc tgttcgacgc
     4801 ctatccggca gaacaaattt gcgccgcgct ttccgcagta ttcagcatca tcttcttcat
     4861 atcctgcctg tcgttcgtca aacaatacaa ggaaacaaac aatgcccgct gaaacgaccg
     4921 tatccggcgc gcaccccgcc gccaaactgc cgatttacat cctgccctgc ttcctttgga
     4981 taggcatcgt cccctttacc tttgcgctca ggctgcaacc gtcgcccgac ttttaccacg
     5041 atgccgccgc cgcagccggc ctgattgtcc tgttgttcct cacggcagga aaaaagctgt
     5101 ttgatgtcaa aatcccacct atcagcttcc ttctgtttgc aatggcggcg ttttggtatc
     5161 ttcaggcacg cctgatgaac ctgatttacc ccggtatgaa cgacatcgtc tcttggattt
     5221 tcatcttgct cgccgtcagc gcgtgggcct gcaagagttt ggtcgcacac tacggacaag
     5281 aacgcatcgt taccctgctt gcctggtcgc tgcttatcgg ctccctgctt caatcctgca
     5341 tcgtcgtcat ccagtttgcc ggctgggaag acacccccat gcttcaaaac attattgttt
     5401 acagcgggca aggcgtaatc ggacacatcg gacagcgcaa caacctcgga cactacctga
     5461 tgtggggcat actcgccgcc gcctacctca acggacaacg aaaaatcccg cccgccctcg
     5521 gcgcaatctg cctgattatg cagaccgccg ttttaggttt ggtcaattcg cgcaccatct
     5581 tgacctacat agccgccatc gccctcatcc ttcccttctg gtatttccgt tcggacaaat
     5641 ccaacaggcg gacgatgctc ggcatagccg cagccgtatt ccttaccgcg ctgttccaat
     5701 tttccatgaa caccattctg gaaaccttta ccggcatccg ctacgaaact gccgtcgaac
     5761 gcgttgccaa cggcggtttc acagacttgc cgcgccaaat cgaatggaat aaagcccttg
     5821 ccgccttcca gtccgccccg atattcgggc acggctggaa cagttttgcc caacaaacct
     5881 tcctcatcaa tgccgaacag cacaacatat acgacaacct cctcagcaac ttgttcaccc
     5941 attcccacaa catcgtcctc caactccttg cagagatggg aatcagcggc acgcttctgg
     6001 ttgccgcaac cctgctgacg ggcattgccg ggctgctgaa acgccccctg acccccgcat
     6061 cgcttttcct aatctgcacg cttgccgtca gtatgtgcca cagtatgctc gaatatcctt
     6121 tgtggtatgt ctatttcctc atccctttcg gactgatgct cttcctgtcc cccgcagagg
     6181 cttcagacgg catcgccttc aaaaaagccg ccaatctcgg catactgacc gcctccgccg
     6241 ccatattcgc aggattgctg cacttggact ggacatacac ccggctggtt aacgcctttt
     6301 cccccgcccc tgacgacagt gccaaaaccc tcaaccggaa aatcaacgag ttgcgctata
     6361 tttccgcaaa cagtccgatg ctgtcctttt atgccgactt ctccctcgta aacttcgccc
     6421 tgccggaata ccccgaaacc cagacttggg cggaagaagc aaccctcaaa tcactaaaat
     6481 accgccccca ctccgccacc taccgcatcg ccctctacct gatgcggcaa ggcaaagttg
     6541 cagaagcaaa acaatggatg cgggcgacac agtcctatta cccctacctg atgccccgat
     6601 acgccgacga aatccgcaaa ctgcccgtat gggcaccgct gctacccgaa ctgctcaaag
     6661 actgcaaagc cttcgccgcc gcgcccggtc atccggaagc aaaaccctgc aaatgacccg
     6721 cgccggcgga tacggatacc gcccgaaatg taaagctcca tgcaagacat tgcaaaaaac
     6781 agcaaaccgg tagggaaata cgctatcaaa aaacattgca gccgtgttaa gataaaccgt
     6841 caaacaatct tttcacgccc cgcccgaaac agggtcgggg cataccctta cgaaaaggaa
     6901 acaccatgag ccgcgtatta ctcgtagatg acgatgccct gctgaccgaa ctgctgaccg
     6961 aatacctgag cgccgaaggt ctgaacgtcc gcagcgttcc cgacggggaa gcaggcgtac
     7021 aggaaatcct gagcgggcag tacgatgtag tcgtattgga ttccatgatg cccaaaatga
     7081 acggcttgga tgtcttgaaa aacgtacgcg cccgaagcac cgtccccatc atcatgctga
     7141 ccgccaaagg cgacgacatc gaccgaatca tcggcttgga aatgggcgcg gacgactatg
     7201 tccccaaacc ctgcacacca cgcgaactct tggcacgcat caatgccatc ctgcgccgcg
     7261 cacaacacag cggcgaacag aacaacgcac ccaacagcat ctccgtcagc gatgtcgtcc
     7321 tgtaccccgc caaacgccag gcatccgtca aagacatgcc gctcgaactg accagcaccg
     7381 aattcaacct gctcgaagtc ctgatgcgcc atgccggaca ggtagtcagc aaagaaaccc
     7441 tgtccgtcga agcactcgac cgcaagctgg caaaattcga ccgcagtatc gacgtacaca
     7501 tctccagcat ccgccacaag ttgggcgatg cctctctgat tcaaaccgta cgcggcttgg
     7561 gctacctgtt tgtcaaaaac tgaagcaacg ggcaaatgaa actgttccaa cgcattttcg
     7621 ccacattttg cgcagttatc gtctgtgcaa tctttgtggc gagtttttct ttttggctgg
     7681 tgcagaacac ccttgccgaa aaccagttca accaacgccg caccatcgaa accactttga
     7741 tgggcagcat catttccgca ttccgggcac gcggggacgc gggcgcgcgc gaaatcctga
     7801 cggaatggaa agacagcccc gtctcatcgg gcgtgtacgt tatacagggc gacgagaaaa
     7861 aagatatcct gaaccggtat atcgacagct acaccatcga acgcgcccgg cttttcgccg
     7921 ccggacaccc gcattccaac ctcgtccata tcgaatacga ccgcttcggc gaagaatacc
     7981 tgttcttcac caaagactgg gacaaactcc aagcccgccg cctgcccagc cccctgttga
     8041 tccccggcct gccgctcgcc ccgatttggc acgaactcat catattgtcc ttcatcatcg
     8101 tcgtcggact gctgatggcg tacatcctcg ccggcaatat cgccaaaccc atcagaatct
     8161 taggcaacgg catggacagg gtggcaaacg gagaacttga aacccgtatc tcccaacagg
     8221 tcgacgaccg cgacgacgaa ttgtcccatc ttgccatcca attcgacaaa atggtggaaa
     8281 aactcgaaaa actcgttgcc aaagaacgcc acctgctcca ccacgtttcc cacgaaatgc
     8341 gctccccgct tgcccgtatg caggcgattg tcggactgat tcaggcgcag ccccaaaaac
     8401 aggaacaata tctcaaacgg ctggaaggcg aactgacccg catggatacg ctggccgggg
     8461 aactgttaac cctgtcccgt ctcgaaactt ccaatatggc tttggaaaaa gaaagcctga
     8521 aactcctgcc cttcctgggc aacctggtag aagacaatca aagcattgcc caaaaaaacg
     8581 gacaaacggt taccctgtct gccgacggaa aaatccccga aaacacaacc atccttgcca
     8641 acgaaagcta cctgtaccgc gccttcgaca acgtcatccg caacgccgtc aactacagtc
     8701 ccgaaggcag caccatcctg atcaacatcg gacaagacca caaacactgg ataatcgacg
     8761 ttaccgacaa cggccccggc gtggacgaaa tgcagctccc gcacatcttc accgctttct
     8821 accgtgcaga ctccagtgcc aacaaacccg gaacaggact ggggcttgca ttgacccaac
     8881 atattattga acagcactgc ggcaaaatca tcgccgaaaa catcaaaccg aacgggctgc
     8941 ggatgcgctt catcctaccc aagaaaaaaa ccggttccaa aacagaaaaa agtgcgaact
     9001 gaccataatg ccgtctgaac cggcttcaga cggcattgca caaacagtta ccccaaaaca
     9061 aacacacagc gggtgcatct ccaaaaatca cgcacatacc gtccgcaaag gaatatatca
     9121 tgtcggcaca aaccgatccc ggctacttct tcatgcccaa ccacattatc ctgataggcg
     9181 cgagcgaacg tccgtacagc ctgggcgaac gcgtactcag caacctgctg agtacgccct
     9241 ttcaaggaaa aatcaccccc gtaaacccgc gccaccacac catagccggg cttcccgcct
     9301 acaccagcct caacaaaatc cccggcaatg cagacctgat tattgccgtt accctacccg
     9361 acagttacga caccctcttc aaaacctgcc gcaaaaagca gctccgacac atcatcctca
     9421 tacaggactg ggacagcctg tctgccgcag aactgcacac cgccgaaact gccatccgca
     9481 aacaccacgg caacggactc aacatcaccg cctgcaccac cgcaggcatc caactgccct
     9541 cactcggact taacatcagt acccaagacg gatacgccgc aggccatacc gccatactga
     9601 ccggcaatgc cgccgtcagc cgccaaatcg acaacatcct gaacaaactc cgtcaaggca
     9661 catcccgcca catcagcctg catcccggca tcagccccat cacatccgcc gattggctca
     9721 accgcttcgg acacagcctg cacaccaaaa ccgccgtcct acaccacaac cctgaagaga
     9781 atcagcgcaa actgttcagc gcaatccgcc aatttacccg ccatacgccg ctgattctcc
     9841 acatcacctg ccgcacgaca gaaaccgacc gtgccgtact gcactgcctc gcccgacact
     9901 gcaacttcct catcagtttc aacgccgacg acctcgaagc cgcactgcgc gcccaactgt
     9961 ccgaccttcc acccctgtcc cgactcgaca tcctgtccga cacgcctgcc gaatggctga
    10021 acgcgcacgc gccaaaaacc ctcgccctcc actttcccaa ccttccccac cacatccgca
    10081 acggacacct gaccggcaca cccacaccct caatctgcca cgacatcgcc tcacgtcagc
    10141 ttgcccgccc cgacacccaa gccgtcctga ccatcctcag cccctccgga cacgaggatt
    10201 acaaaaaaac agcacgcgcc cttatccgcc tgtccgaaca gagcgccaaa cccctgctcg
    10261 tcagcagccc cttttcagac ggcataacac atttcgacac ccccactcag gcaatccgca
    10321 ccctttccta ccgcaacacc gccgccgccc tgaaacaggc acagctcgac attgcaccgc
    10381 cgcagccatg ccgtctgaaa acaccgcaac cccaaaacat caaaaaagcc ctcgaagccg
    10441 ccgaccccgc cctaatcgcc gaagccctgc acctcccccc ctaccggcac accacccata
    10501 acgccgtcca attccaattc ggcagccact ccctctatgg cgacatcctg accgcacgtt
    10561 gcaacggaca aaccactgcc gtactcccgc cgtttaccac gctcgacagc cgccaccttg
    10621 cccgctttgc cgaactcgac ggcacacaaa ccctcgacca gttcctgcac acactgacca
    10681 tcattcccga ataccgccaa cacattctcg gcatcaccct caacctaaac ggcggacaat
    10741 acagcagcga tttcatctta aagacacccg aaacacacga cacgcccaaa cgcaagaaca
    10801 caggcaaagc cgcccaaacc ctcgaacatg ccgccgcaaa aatgcagagt gccgccgcat
    10861 acctgaaaca caaaaacccg acagccgccg aatttctccg ccacacaagc gaagccgccg
    10921 cagaactgct cggcagcaaa accgaaaccg gagcagccgt acccaacgta cttgccccct
    10981 atcccgcagc ataccccaaa acactgtccc taagaaacaa cacgaccgtt accattaccc
    11041 ccattttgcc cgaagacgca gaagccaaac agcagttcgt ccgcagcctc ggtcccgaag
    11101 cacggtacac acgcttcatg acccacacca acgaactgcc cgcagccacg ttggcacgcc
    11161 tgtgcaaccc cgattaccac tgtgaagccg catggacggc aaaggatgcc gacagcaaca
    11221 tcgtcgccgt cgtccgccac agccgcctga accgcaacga atgcgaattc ggcatcacac
    11281 tggcggaaca tatgcgcggc agcggactgg cacagaaaat gatggaactc atcatccaaa
    11341 ccgccgcaca gcaaggctac cggactatga gtgccgacat tctcaaaacc aataccccca
    11401 tgatcaaact tgctgaaaaa tcaggattta ccctcaagga atcggacacc gaaaaaaacc
    11461 tgtaccgcgc atatctgaac cttgcggcag acaaaacaac agaaaaaaca aataaaaact
    11521 tgcgcaccga ccacaaaata acctaaaatc ggcagtttcc atatatccga ttttctcaaa
    11581 aggactcaaa atggtagtta tccgtttggc acgcggcggc tcgaaacacc gccccttcta
    11641 caacgtcatc gttactgact cacgcagccg ccgcgacggc cgcttcatcg aacgcgtagg
    11701 cttctacaac cccgtagcca acgaaaaaca agagcgcgtc cgcctcaatg cagaccgcct
    11761 gaaccactgg attgcacaag gcgcgcaagt cagcgactcc gttgcaaaac tgattaaaga
    11821 acaaaaagcc gcctaattca catttgccgc catgacagac actcaaaacc gggtagccat
    11881 gggctacatc aaaggcgtat tcggcataaa aggctggttg aaaattgccg ccaacaccga
    11941 atattccgac agccttttgg actaccccga gtggcatttg gtcaaggacg gcaaaaccgt
    12001 cagcgttacc cttgaagccg gaaaagtcgt caacagcgaa ctccaagtca aattcgaagg
    12061 cataaacgac cgcgacttgg cattctcatt gcgcggttac accatcgaaa taccccgtga
    12121 agcattcgcc ccgacagaag aagacgaata ctactggaca gacttggtcg gcatgaccgt
    12181 cgtcaacaaa gaccataccg ttttaggcaa ggtaagcaac ctgatggaaa ccggcgcaaa
    12241 cgacgtattg atgattgacg gagaacacgg gcagattctg attccgttcg tttcccaata
    12301 tatcgaaacc gtcgataccg gcagcaagat tattactgcc gactggggtt tggactactg
    12361 atgcttatcc aggcagttac cattttcccc gaaatgttcg acagcattac ccgctacggc
    12421 gtaacgggac gcgcgaacag acagggaatc tggcagtttg aagcagtcaa tccccgaaag
    12481 tttgccgaca acagattggg ctacatcgac gaccgcccgt tcggcggcgg cccgggaatg
    12541 attatgatgg ctccgccgct tcatgcagca atagaacacg ccaaagcaca atcctcccaa
    12601 gctgcaaaag tcatctacct cagcccccaa gggaaaccgc tgacacacca aaaagcggta
    12661 gaactggcag aacttccgca tctgattctg ctgtgcggac ggtatgaggg catagacgaa
    12721 aggcttctgc aaagcagcgt cgatgaagaa atcagcatcg gagacttcgt tgtttccggc
    12781 ggagagcttc ccgccatgat gctgatggat gcggtattga ggctcgtacc cggcgtattg
    12841 ggcgatatgc agtctgccga acaagattcg ttctcaagcg gcattttgga ctgcccccac
    12901 tacaccaaac ccttagaatt tcaaggcatg gctgttccgg aagtattacg ttccggcaat
    12961 catggcttga tagcggaatg gcggttggaa caatcgctgc gccgcacctt ggagcgcaga
    13021 cccgatcttt tggaaaagcg cagtttaatc ccaaaggaat cccgcatctt gaataaaatc
    13081 ctacaagagc aacgggaaat ccaatcataa tttaggaaaa aaacaatgaa cctgattcaa
    13141 cagctcgagc aagaagaaat tgcccgcctg aacaaagaaa tccccgaatt cgcaccgggc
    13201 gacaccgtag tcgtatccgt acgcgtcgtg gaaggtaccc gcagccgtct gcaagcctac
    13261 gaaggcgttg tcattgcacg ccgcaaccgt ggtctgaaca gcaacttcat cgtccgtaaa
    13321 atttccagcg gcgaaggtgt tgaacgtact ttccaactgt actctccgac cgtcgaaaaa
    13381 atcgaagtca aacgccgtgg cgacgtacgc cgtgccaaac tgtactacct gcgcggtctg
    13441 accggcaaag ctgcacgcat caaagaaaaa ctgcctgcac gcaaaggttg attcaaaccc
    13501 gttttcccca atgccgtctg aaccttctga cggcatttct tattgccgca acatccgcaa
    13561 cggaacagca ccaagaagca caccccttcc ggcacatccc gaatccccgt ccgaaccgaa
    13621 caaaatttac agaccaaacg ttatacagta tcatatcagc catataataa taaccgcttt
    13681 accgccttcc ctttcaaaac cgcaaatcat catgacacct tcccttttac tatcaggatt
    13741 gaccttccgc ctcgtccttg ccctgattgc cgtatccctt ttatggggcg tttacttttg
    13801 ggcagtatcc gcatgagcat cattgtcgaa aacctgaccg tcagctaccg ccgccgacct
    13861 gccgtgcacc atgtggacat tacttttgaa gaacatagta tgtgggcggt tttcggtccc
    13921 aacggcgcag ggaaatccac ctttctcaaa tccttaatgg gattgcagcc tatcgatacg
    13981 ggcagcatcc ggctggacgg attgacccgt cagaacattg cctaccttcc ccagcagtcc
    14041 gacatcgacc gctcgcagcc catgaccgtt ttcgacttgg cggcaatggg gctatggtat
    14101 gaaatcggtt ttttcagagg aataaatgcc gttcaaaaac aacgcgttca cgaatctttg
    14161 gaacgcgtcg ggatgcggca gtttgccggc cgccaaatcg cccacctctc aaacggacaa
    14221 ttccagcgtg tcctttttgc ccgaatgctg gttcaaaacg ccaaattcct acttctagac
    14281 gaacctttca acgctgttga tgcacggacg acctacgaac ttctcgacgt attgcagaaa
    14341 tgccattgcg gcggacacgc catcatcgcc gtactgcacg attacgaaca agtccgtgcc
    14401 tactttccca ataccctgct gctcgcccgc gaaaaaattg cggcaggcgc aaccgagacc
    14461 attctgacag aaccctacct cgcccaagcc aacgccaaaa tgcagcaaca ggaaagcccc
    14521 gagtggtgcg cgtcataaat gccgtctgaa accgaaaaac catgaacctc tacgacctgc
    14581 tccttgcccc ctttgcagaa tttgacttta tgcgctacgc cctcgtatcc gtcttctgcc
    14641 tgtccctcag tgccgcaccc gtcggcgtat tcctcgtcat gcgccgtatg agcctgatag
    14701 gcgacgcatt gagccacgcc gtcctgcccg gtgccgccgt cggctacatg tttgccggct
    14761 taagcctgcc cgccatgggt ttgggcggcg tagccgcagg tatgctgatg gcactgcttg
    14821 ccggactcgt cagccgcttc accaccctga aagaagatgc caactttgcc gccttttatc
    14881 tcagcagcct cgccatcggt gtagtcctcg tcagcaaaaa cggcagcagc gtcgatctgc
    14941 tgcatctgct tttcggctct gtacttgccg tcgatattcc tgccctgcag ctcatcgccg
    15001 ccgtatccag cctcacgctc attacccttg ccgtcatcta ccgcccgctc gtactcgaaa
    15061 gcatcgaccc cctgtttctc aaatctgtcg gcggcaaagg cgggctttgg cacgtcctct
    15121 ttctcgtcct ggtcgtcatg aacctcgtat ccggctttca agccctcggc acactcatgt
    15181 ccgtcggact catgatgctg ccagccatta ccgcccgcct gtgggcgaag cacatgggcg
    15241 cactcatcct cctatccgtt ctgacagccc tgctgtgcgg cttgagcggg ctgctcattt
    15301 cctaccacat cgaaatccct tccggccccg ccatcatcct ctgttgcagc gtcctttatc
    15361 tcttttccgt catactcggc aaagaaggcg gcattctgac caaatggctc aaaaaccacc
    15421 gccaccacac cacctgaccc cgcaagccgc ttgaacaaac aggcggcttg ttttaccata
    15481 acagatgtta cgttatatca caggagaaca gttatgaaac acctcaaact cacccttatt
    15541 gccgcattgc tgaccactgc cgcaactgcc gccccccttc cggttgtaac cagtttcagc
    15601 attttaggcg acgttgccaa acaaatcggc ggagagcgcg tatccataca aagtttggtc
    15661 ggagccaacc aagatactca tgcctatcac atgaccagtg gcgacattaa aaaaatccgc
    15721 agtgcaaaac tcatcctgct caacggctta ggacttgaag ccgccgacat ccaacgtgcc
    15781 gtcaaacaga gcaaagtatc ctatgccgaa gcgaccaaag gcatccaacc cctcaaagcc
    15841 gaagaagaag gcggacacca tcatgaccac gaccatgacc acgaaggaca ccaccatgac
    15901 cacggcgaat ttgacccgca cgtctggaac gaccccgtcc tcatgtccgc ctatgcccaa
    15961 aacgtcgccg aagccctgat aaaggccgac cccgaaggca aagtttatta tcaacaacgc
    16021 ttgggcaact accaaatgca gctcaaaaaa ctgcacagcg acgcacaagc cgcatttaac
    16081 gccgtccctg ccgccaaacg caaagtcctg accgggcacg atgccttttc ctacatgggc
    16141 aaacgttacc atatcgaatt catcgccccg caaggtgtga gcagcgaagc cgagccttca
    16201 gccaaacaag tcgccgccat catccgacaa atcaaacgcg aaggcatcaa agccgtattc
    16261 accgaaaata tcaaagacac ccgcatggtt gaccgtatcg ccaaagaaac cggtgtcaac
    16321 gtcagcggca aactgtattc cgacgcactc ggcaacgcgc ccgcagacac ctacatcgga
    16381 atgtaccgcc acaacatcaa agccttgacc aacgcgatga agcaataaaa ccgtttgatt
    16441 ggtttgaatt tgaaaaggca aaaggccgtc tga
//
LOCUS       DAWFAD010000036        16143 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWFAD010000036 DAWFAD010000000
VERSION     DAWFAD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16143
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.036"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(246..3035)
                     /gene="ileS"
                     /locus_tag="WGO49_000851"
     CDS             complement(246..3035)
                     /gene="ileS"
                     /locus_tag="WGO49_000851"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ0305982.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVACQKKDFIRLGVLGDWDKPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVGYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYISETPRVAGMSVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVAQRIEEKGIE
                     AWFSLDKGELLSAEDCEHYDKLSDTMDVWFDSGSTHYSVVKQREELDWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDQNGRKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYAVVLARQLQERLAGDYYPRYTFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFALLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGREGETRHYA"
     gene            complement(3176..4096)
                     /gene="ribF"
                     /locus_tag="WGO49_000852"
     CDS             complement(3176..4096)
                     /gene="ribF"
                     /locus_tag="WGO49_000852"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ0305983.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSDCFQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKCW"
     gene            complement(4623..5918)
                     /gene="tyrS"
                     /locus_tag="WGO49_000853"
     CDS             complement(4623..5918)
                     /gene="tyrS"
                     /locus_tag="WGO49_000853"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ0305984.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVKSIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLYQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLNGEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(5981..7859)
                     /locus_tag="WGO49_000854"
                     /pseudo
     CDS             complement(5981..7859)
                     /locus_tag="WGO49_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(7870..7974)
                     /locus_tag="WGO49_000855"
                     /pseudo
     CDS             complement(7870..7974)
                     /locus_tag="WGO49_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7990..8196)
                     /locus_tag="WGO49_000856"
                     /pseudo
     CDS             complement(7990..8196)
                     /locus_tag="WGO49_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8234..9325)
                     /gene="ychF"
                     /locus_tag="WGO49_000857"
     CDS             complement(8234..9325)
                     /gene="ychF"
                     /locus_tag="WGO49_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ0305985.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVAEDGFENNPH
                     LERLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(9439..11115)
                     /locus_tag="WGO49_000858"
     CDS             complement(9439..11115)
                     /locus_tag="WGO49_000858"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ0305986.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAVLLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGVGGADLARKVVNAIENQPNNFNFSYDVELSIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSSGAGFIVALCGNMMKMPGLPKVPAAEKIDVDAEGVIHGLF"
     gene            11290..11721
                     /locus_tag="WGO49_000859"
     CDS             11290..11721
                     /locus_tag="WGO49_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0305987.1"
                     /translation="MSSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPID
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(11952..12647)
                     /locus_tag="WGO49_000860"
     CDS             complement(11952..12647)
                     /locus_tag="WGO49_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ0305988.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGGQPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHNPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(12665..13165)
                     /gene="hpaC"
                     /locus_tag="WGO49_000861"
     CDS             complement(12665..13165)
                     /gene="hpaC"
                     /locus_tag="WGO49_000861"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ0305989.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINMLADEHQDVAEHFAGLTNLSPEERFAY
                     HIWHYGKTGQPEIEGALAHLHGHIVGQHEIGTHFVFYVRLDEIKNCVGKRPALLYFRR
                     QFRSLD"
     gene            complement(13280..13720)
                     /gene="nadR"
                     /locus_tag="WGO49_000862"
     CDS             complement(13280..13720)
                     /gene="nadR"
                     /locus_tag="WGO49_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ0305990.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(14063..14572)
                     /locus_tag="WGO49_000863"
     CDS             complement(14063..14572)
                     /locus_tag="WGO49_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ0305991.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(14651..14726)
                     /locus_tag="WGO49_000864"
     tRNA            complement(14651..14726)
                     /locus_tag="WGO49_000864"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14691..14693),aa:Lys,seq:ttt)
     gene            14937..16016
                     /gene="apbC"
                     /locus_tag="WGO49_000865"
     CDS             14937..16016
                     /gene="apbC"
                     /locus_tag="WGO49_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ0305992.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIAAAVADSVQEILMPETGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICSNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALSIADKGKDFSSRFPKIVVE"
BASE COUNT         3661 a         4417 c         4343 g         3722 t
ORIGIN      
        1 taatctcgaa atccgtcatt cccgcacagg cgggaatcca gacccacgac gcggcgggaa
       61 tctatcggaa atgactgaaa cctcgagatt ctagattccc acttccgtgg gaatgacggt
      121 tcagttgcat tccgacaaca ccgcaatctt gaaacccctc cgccgttata aagacaaatt
      181 gcggcacaaa aaatgccgtc tgaaatgctg ttcggcggtt tcagacggca tttgctcaaa
      241 ctttatcagg cgtaatggcg cgtttcgcct tctctgccga cattctctgc acagcgtttg
      301 cagacggttt catagcctgc aaccgcgccc acatcgcggg tgtagtgcca gcagcgttcg
      361 catttttcac catcactggc tttagcggca acggcaagtt cgctgcctac tttcacttct
      421 gctttagaca ccagcaaggc aaagcgcaat tcttcgccca aagcgttcag gtagccggcc
      481 atttcttccg gcgcagtgat ttcggcttcg gcttgcaagg acgaaccgac ggttttgtcg
      541 gcgcgcaaag gctcgatggc ggcggttacc gcttcgcggg cttcgcggat tgccgtccat
      601 tttttcacca gttcggcttc ggctttttcg ttgatggtcg ggaactcgtg ccaggtgtgg
      661 aagaggacgc tgtcttcttc gccgccgccg atgatgtccc acgcttcttc gccggtaaag
      721 cacaaaatcg gcgcaatcaa gagaaccaaa ctgcgcgtga tgtggtacag ggcggtttgc
      781 gcgctgcggc gggcgcggct gtcggctttg gtggtgtaga ggcggtcttt caggatatcg
      841 aggtagaacg cgcccaagtc ttccgagcag aaagaaacaa tgtcttttac agcaaagtgg
      901 aaggtatagc gcggatagta atcgcctgcc agacgctctt gcagctgacg tgccaatacc
      961 acggcgtagc ggtcgatttc caccatgtct gcctgttgca cggcatcttc aataggattg
     1021 aagtcgctga ggttggcaaa caggaagctc aaggtgttgc ggatacggcg gtagctttcg
     1081 gttacgcgct tgaggatttc tttggaaatc gccaattcgc cgctgtaatc ggtggatgcc
     1141 gcccacaggc gcaggatgtc cgcgccgaac tcgttataca cttcttgcgg cgcgacgacg
     1201 ttgccgatgg atttcgacat tttgcgcccg ttttgatcca ccacgaaacc atgggtcagc
     1261 agctgtttgt acggcgcgcg acccattgat gaggcgcagc cggtcagcat ggacgactga
     1321 aaccagcccc ggtgttgatc gctgccttcg aggtacaagt cagcaggcca gtccaattct
     1381 tcgcgctgtt tcacaacgga ataatgggtc gaaccggagt cgaaccatac gtccatcgtg
     1441 tcggagagtt tgtcgtaatg ttcgcaatct tccgcgctca acaattcgcc cttatcgagg
     1501 gagaaccaag cctcgatgcc tttttcttcg atgcgttgcg cgactttttc caaaagttcg
     1561 gcagagttcg gatgcagctc gcccgtttct ttgtgaacaa agaaagtcat cggcgtgccc
     1621 caatagcgtt ggcgcgaaac cacccagtcg gggcggcctt caatcatggc ttccaaacgc
     1681 gcacgacccc atgatgggaa aaattcagtg tcatcaacgg ctttgatggc tttatcgcgc
     1741 agggttttac catcggcacc tgctttatcc ataccgacaa accattggcc ggtcgcacgg
     1801 taaatcaacg gcgttttgtg acgccagcag tgcgcgtagc tgtgttcgat tttgctgctt
     1861 gccaagaggt tgccggtttc ttccaaccat tgcaggataa cgggattggc ctcccaaacg
     1921 ctcatgccgg caacgcgcgg cgtttcactg atgtatttgc cttcggcgtt gacggggttg
     1981 taaagctcga tgccgtattt attgcagacg gcgtagtctt ccaaaccatg agcaggcgca
     2041 gtatgcacca aaccggtacc ggcgtcggtg gtaacgtggt cgccgttgag catgggaata
     2101 tcgcgttcga ggaacggatg attcatgtgc aggttttcca gcttgtcgcc ggtggtttcg
     2161 gcaagaatag caatgccgtc tgaaaaaccg taacgtttga gcgcgtcttc tgccaaatct
     2221 ttcgccagca ccaatttgcc tttgggcgta tcgattaatt gatacaccac gtccgcaccg
     2281 gcagacacgg cttggctcgc tggcagcgtc caaggcgtgg tcgtccagat gacggcaaac
     2341 gctttgccct cgatgcctgc caaaccgaat gcagcggcaa gcgcagcggt gtctttaaac
     2401 ggatagccca cgtcaatcgc aggcgatact ttgtctttgt attccacttc ggcttcggcc
     2461 aatgaagagc cgcagtccaa gcagaactga accggtttcg cgccgcggta gagatagccg
     2521 gatttgtaga tttcgccgag catacgcacg gtatcggctt cggttttaaa atccatggtc
     2581 aggtagggct tgtcccagtc gcccaacacg cccagacgga tgaagtcttt tttctggcag
     2641 gcaacttgtt cggcggcata ttcgcggcac aattcgcgga aacgcgcttt gggcatgtct
     2701 ttgccgtgca gcttttctac catcacttcg ataggcaaac cgtgacagtc ccaacccggc
     2761 acataaggcg cgtcaaaacc ggcttgggtt ttgctgcgga taatgatgtc tttgagaatt
     2821 ttgttgacgg cgtgaccgat gtggatgtcg ccgttggcat acggcgggcc gtcgtgcagg
     2881 atgaacttcg gacgaccttt ggcgatttcg cgcagttttt ggtagcgttt ttgctcgtac
     2941 cagcttttca gccatgcagg ctcgcgcttg gcaagattgc cgcgcatcgg aaacgggctt
     3001 tcgagcaggt ttacggtttt gctgtaatcg gtcatttttt aatctctatt gttacaatat
     3061 ttcggttcag acggcattgc gcctcaaaca gtatttcaca acgggaaacc cctatgccgt
     3121 ctgaacagtt aaaaggttga ttgtagccca atcggatggt ttgtataagg tttttctacc
     3181 aacactttgc ggcttccata tcggcttcaa tctgcctttt cagttcttcc ataccgtcaa
     3241 acttttcctc atcgcgcagt ttgtgcagga agcggacgtt caaccgttgt ccgtacaaat
     3301 cgccttgaaa gtcgaacagg tggacttcca gcttttgaaa acagtcgcta tcaacggtgg
     3361 gattgaagcc gaagctcgcc acgccgcgcc gcgtgccgaa cgcgccgtcc gcttcgacga
     3421 cgaacacgcc gccgagtgca taacggtggc ggggcaggcg gatgttggca gtcggggcgt
     3481 ttaaggtgcg tccgagtttt ctgccgtgca ccaccctgcc gctcaagacg tagtcgtgtc
     3541 ccaaaagttt tttcgcatag gcaaggttgc cgtctgaaag ggcttgtcgc acggcggtac
     3601 tgctggtgcg gatgtcttcg acgatgacgg aaggcgtacg ctcggtctgc atatcgggct
     3661 gttgtgccaa aagttcaaaa cagccttccc gccccgcacc gaaacggaaa tcgtcgccga
     3721 cgagcaaata acgcgtattc aaggtttgac gcagcaggcg gtcgataaac ccttgcgcgg
     3781 atatttcgga aaaattttga tcgaaacgca aaacccagac ggcatcgaca cagcctgtgc
     3841 cttccaataa ttcgagcttg gtgcgcaggg ggctgatccg acacggtggc atcctgccgg
     3901 tgcggagtgc gaaaaattct ttgggttggg gttcgaaaac gacggtcacg acgggtaatc
     3961 cgcgcgtatc ggcttcgagg cggagttttt ggaggatgtg tttgtgtccg aggtgtacgc
     4021 cgtcaaaatt gcctatggtt acggcggccc cgtgcggaaa gtcgggcgcg ttgtgccgcc
     4081 ccagcctgat tctcattgtt atgttcgggt atgtggtgaa acaggcggtc attgtaaacg
     4141 gtattgcggt ttatagacag tgtgccgccc gttacgcccg cccgacgcgg gaaaagtagg
     4201 caaatttccc gcagccgaac gcgccaaacg cataaagcgc gcagcaggcg cggtgctatg
     4261 tgttgaaaca tcgccccaaa cctgatgcaa catcagtgaa aatcgttctt ttttaaccag
     4321 gtaaaccgag acaagcaacc ctccgccgtc attcccgcgc aggcgggaat ccggacctgt
     4381 ccgcacagaa acttatcgga taaaaacagt tgctcaaacc ccgagattct agattcccac
     4441 tttcgtggga atgacggttc agttgcgttc cgacaacacc gtaatcttga aactcgtcat
     4501 tcccgctcag gcgggaatct agaacgtgga atctaagaaa ccgttttgcc cgataagttt
     4561 ccgtgcggac aggtccggat tcccgcctgc gcgggaatga cggcatttct gcggcaatcg
     4621 gattatttcc aaaccaaaag cgcgtggttg cgtttgccgc gccgaaggat ggtgtatttg
     4681 ccgaaacgtt tgtgttcgcc gttcagcagg caggcatcgt cggggcgttc ggcggcgtgg
     4741 ttggggttgt tggcttcggc aggtttgccg ttgagcaaaa ccgctttgct gttcacaaag
     4801 ccgcgcgctt ctttattgga ggatgccaaa ccggttttga ccaaggcttc gacgacattg
     4861 atgccgtctg aaacttcaaa tgcaggcagg ccgtcgaggg cgagctgctc gaagtcgctt
     4921 tcggtcaggc tgctttggtc ttcggcaaac aggctttcgg aaatgcgctg cgcggcggca
     4981 agggcttctt cgccgtgaat caggcgggtc atttcttcgg cgaggatgcg ttgcgcttcg
     5041 ggcttgctgc cgctggcttt atctttcgct tcgatagcgt cgatttcttc gatggacagg
     5101 aaggtaaagt atttcaggaa tttatacaca tcggcatcgg cgactttcag ccagaattgg
     5161 tagaactgat agggcgaggt ttttttcgcg ttcagccaca ccgcgccgcc ttcggttttg
     5221 ccgaatttgg tgccgtctga tttggtaacc aaaggcagag tcagtccgaa cacttgtttt
     5281 tggtacaggc ggcgggtcag gtcgataccg gcagtgatgt taccccattg gtcggagccg
     5341 ccgatttcca aaaccgcgcc gtggcgtttg ttcaactcgg cgaagtcgta accttgcagc
     5401 agggaatagg cgaactcggt gaaggaaatg cctgcgccgt cgcggtcgat gcgctgtttg
     5461 acggattctt tgttcagcat ggcgttgacg gagaaatgct tgccgatgtc gcgcaggaag
     5521 tcgaggcagt tcattttgcc gaaccagtcg gcgttgttcg ccataatggc ggcgtttccg
     5581 ccttcaaagc tcaagaaagg ggttaattgg ctgcggatgc ttttcaccca gccggcaacg
     5641 gtttcggcgg aattcaagct gcgttcggcg gctttgaagc tggggtcgcc gatcataccg
     5701 gtcgcaccgc ccaccagtgc aatcggcgta tgccccgcct gttggaagcg gcgcaatgcc
     5761 aatacgggca gcaggtgtcc gatgtgcagg ctgtcggcgg tcgggtcgaa gccgcaataa
     5821 agggaaattt tttgttcgtt caacaaagcg tctaaggctt cgatgtcggt ggtttgcgcg
     5881 attaggccgc gcgattgcag gtcttggatg acgctcatcg gtctctttca aaaaaaatta
     5941 gcggttttgc aaaccgccga ttgtaacaaa tttaagcgaa tcaatggtta tggcgcgtat
     6001 cgagaaaccg ttgtttttcg gaaaaacgct tcgccaattc cgtgccgccg taagggttga
     6061 tgtggtcttt gtccgagtaa accggcaatc cgccgatttg gaaatttgcg gggatatagg
     6121 cggcggcatc aataatatag acgttggggt atttggctgc caattccctg atgcgtgcat
     6181 tggctttcag agtgctttcg tcgtccgggc gcagggtttg gcggtaaccc ggtatgcgtg
     6241 aagacaagat ataggcgcgt tgtacgttgt aagacgaggc aaggttgtcc gccatcaggt
     6301 aaacggcttg tttttcggat gagagtttgt gcagcatacg gtcgaatttt tggaaaaaac
     6361 cggcatcgta ggcaagggag cggctgtttt cgggcatttg gctgccccag cgcatcgcca
     6421 aaaccacttt tgaatacagg ggcaggtgtt cttcggcata gcggtaaacg gcgcggcagg
     6481 cggcccagtt ttggaacaca cgggacgcgt agccttccac ataggcgcaa gcgtcggcgg
     6541 aaaccatagt ggcggaccat ttttcttttt tgcccacggc atcgaagaat gttttgtaat
     6601 ggtcggcgtg ggagtcgccc aaaaccagca gctccggctg tttttccgta tccccccaca
     6661 ggcattgttt gccggtattg ttgtggcagg aggtgttgga acgcgtcagc cccaagcggt
     6721 cgtattgcgc cataaacggc agcctcatcg cgaaaaacga gcccgccccc aaaatgagca
     6781 taggcaaggc ataaatccat aaaacggatt gtgcgaacga acctttccat tttttaaacg
     6841 gcttttcgat gcagtgataa gaaatcaggg aaagcagcaa tatcaggacg attgctgccg
     6901 ctgttgaata aggcggcagg ttgtccgggc cgatatagcg cataaaggcc aatatcggcc
     6961 aatgccacag ataaagcgaa taggaaatca aaccggcggc aacagtgatt ttccattgga
     7021 aaaatttttt aagcgggtgt tcgtaatgat tgaaataaat cagcgcggca acagccagac
     7081 agggaatcaa agcggcgggt cccgggaaat aggcggtttg tttcgaatag gcaaacaggc
     7141 aggcggacag tatgcacgcg gaaaacaatg cgccggcggc ggcacagcat ctgccaacgg
     7201 cgagattccg ctgttgccgg taccgcatcc acacggcggt cagcgatccg accagcattt
     7261 cgcaggcgcg caggtggggc aggtaatatt tatcgaacgc ggaaggcata aaggaagcgg
     7321 caaggcttaa ggcgcacaat gcggcgagga agccgaactg tacgcgcggg cttttgcggg
     7381 cgacaagcag cagcaatatc ggaaagacaa agtaaaattg ttcttcgacc gacaaagacc
     7441 aaatgtgcag caagggcttt tcttcctgcg cgggatcgaa ataatccttc ccccttgcaa
     7501 aatacaggtt ggaggcgaaa cccaaggcgg tcagcgcgga tttccacaaa agaaagaaat
     7561 catctttggt gaataaaaag aagccgccta tcagcgttgc cgccaatacg gcgaaaaatg
     7621 cgggcaaaat ccgtttgatg cggcggatat aaaatgcctt cagggaaaac ctcccccccc
     7681 cccgacattt cgcggtgaag aatcatcgtc atcaaaaagc ctgaaatcac gaaaaatata
     7741 tcgacaccga gaaacccgcc cggcagccaa tttttttcga tatggaacac gatgacggac
     7801 aagacggcgg cggcgcgcaa tgtgtcgatg tccgggcggt agggtaaggc ttggctcata
     7861 atgtttttat agtggattaa atttaaacca gtacggcgtt gcctcgcctt gccgtactat
     7921 ctgtactgtc tgcggtttcg tcgccttgtc ctgatttaaa gttaatccac tatactcgaa
     7981 acgcggcggc gcaaatgccg tctgaaaggt cattttgtat cggggctcgg gatattcgga
     8041 atgccggacg gctttttgca acagcggggc aggcggtttg ttttgcagga atcggggagg
     8101 gcaaatcgga aatgcgggcg ggattttatt ttgatgcagc cgcattccgg cggtatggaa
     8161 acgccgaaag ccaccaaaac cgacttcaga cggcattttc ggcaaagccg tctgaaaacc
     8221 tgccgcattt gggttacacg ttaaacaaaa agtgcatcac atccccatcc tgcacgacgt
     8281 attccttgcc ttccacacgc attttgccgg cttctttggc tttggcttcg ccgccgagcg
     8341 agacaaagtc gtcgtaagaa atgacttggg cgcggatgaa gccgcgctca aagtcggtgt
     8401 gaatcacgcc ggcggcttgc ggggcggtgt cgcctttgtg tatcgtccaa gcgcggactt
     8461 ctttcacgcc ggcggtaaag taggtttgca gccccaaaag gtcgtagccg gcacggatca
     8521 ggcggttcag gcccggttct tccaagccca tttcggcgag gaactcggct ttttcgtcgt
     8581 cttccaattc ggcgatttcg ctttccattg cggcgcaaac ggcgacgacg ggggcgtttt
     8641 cttttgccgc caattctttc aggcgttcga ggtgcggatt gttttcaaaa ccgtcttcgg
     8701 cgacgttgcc cacatacatc gccggtttgg cggtcagcag gaacagcggt ttgagcatcg
     8761 cgcgttcttc cgcgtccaaa ccgaaggaac gcacgggttt gccttcgtcc agatgcggca
     8821 gcagtttttt gcacaaatcg accagctttt gcgcgtcttt gtcgcctgaa cgggcgcgtt
     8881 tttcttcgcg gacgatggct ttttccacac ttgccaggtc ggcaagtgcc aactctgtgc
     8941 cgatggtttc gatgtcggca atcggatcga cgcggcctgc aacgtggacg atgttgtcgt
     9001 cgtcaaagca gcgcacgaca ttcacaatcg catcggtttc gcggatgttg gcaaggaact
     9061 ggttgcccaa gccctcgcct ttgctcgcgc ctgcaaccaa accggcaata tcgacaaatt
     9121 cgacgatggc aggctgcatt ttttgcggat tgacgatttt tgccaattcg gccatacgcg
     9181 gatcggggac ttcgacgatg ccgacgttgg gttcgatggt acagaaagga tagtttgccg
     9241 cttcgatacc cgattgggtc agcgcgttaa aaagggtgga tttgccgacg ttgggcaaac
     9301 cgacgatgcc gcatttcaaa ctcatgtttt ttcctgaaaa tagagaaatt taacggcgga
     9361 ttatagcata ccgccgcccg cgttccgaaa aaatgccgtc tgaaacggct tcagacggca
     9421 tctggtttca aaaaccgttc agaacaagcc gtgaatcacg ccttctgcgt ccacgtcgat
     9481 tttctcggcg gcgggaactt tgggcagacc cggcattttc atcatattgc cgcacaacgc
     9541 aacgatgaaa ccagcaccgg aagaaacggt aatgccgcgc acggtaatgc ggaagccttc
     9601 agggcagccc aagagtttgg cgttgtcgct caaagaatat tgggttttcg ccatgcaaat
     9661 cggcattttg tccaagccca gtttttccag cgaggcgatt tcggcagacg cttccgcgct
     9721 gaaatcaaca tcttccgcgc cgtacacttt ttgggcaatc gcacggattt tgtctttaat
     9781 actcagctca acatcgtagg aaaaattgaa gttgttcggt tggttttcga tggcgttgac
     9841 gactttgcgc gccaagtctg caccgcccac gccgcctttg ccccacactt cggtcaagga
     9901 aacttcaacg ccgtgttcgg cacaggcttt ttcaatcatc gccaactcgg catcggagtc
     9961 ggacacgaag cggttgagcg caacgacgac gggcagtccg aatacgtttt tcaggttgga
    10021 aatgtgtttc agcaggttgg gcaaaccttt tgccaaggct tcgaggtttt cttcaccgag
    10081 gttggcacgt tccacgccgc cgttgtattt caaagcgcgg acagtcgcca cgacgaccgc
    10141 cgcatcaggt ttcaaaccgg caaggcggca tttgatgtcg cagaattttt ccgcgcccaa
    10201 atccgcgccg aagcctgctt cggttacggc gtaatcggca aggtgtttcg ccagacgggt
    10261 tgcggttacg gagttgcagc cgtgggcgat gttggcgaac gggccgccgt gtacgaaggc
    10321 gggcgtgcct tcgatggttt gcaccaaatt gggcttaatc gcatctttga gcaggaccgc
    10381 catcgcgccg tgtgccttca aatctttggc gtaaacgggg ctgccgtctt tggcgtaggc
    10441 gacgaggatg ttgcccaaac gctctttcaa atcgctgatg tctttggcaa ggcagaatac
    10501 cgccatcact tcggaggcaa cggtgatatc aaaaccgtcg gggcgcatca caccgtcaac
    10561 gggtttaccc atgccatcga tgatgttgcg caactggcgg tcgttcatat cgaccacgcg
    10621 ccgccacagc acgcgtttgg ggtcgatgtc caactcgttg ccctgataga tatggttgtc
    10681 gagcatcgcg gcaagcagat tatttgccgc accgatggcg tgaaaatctc cggtgaagtg
    10741 caggttgatg tcttccatcg gcaaaacttg ggcatagccg ccgcctgccg cgccgccttt
    10801 cacgccgaac accggcccca gagaaggttc gcgcagggca atcacggcat ctttgccgat
    10861 gtggcgcaac gcgtccgcca aaccgatggt tacggtggtt ttgccttcgc ccgccggagt
    10921 cggattaatg gcggtaacca aaatcagcct gccctgtttt tgcggcagtt tgaacgcttc
    10981 ggcaggattg attttcgcct tgtaatgacc gtaaggctca atgttgtcgg cattcagacc
    11041 cagcttggcg gcaatttcgc caatcgggcg catggtggag gattgggcga tttcggcatc
    11101 ggttttgaag ctcatgattt tcctttagaa atgaggaggg acatgccgtc tgaaagcatc
    11161 aggcgacaaa caggtggatt gaaaataata tcaggcatat tataacgtta tccgcaccaa
    11221 acccgcagtg aaatttttga cgctgcaaca aaaataccgt tcatattgtt cacaatccaa
    11281 ggagaaaaca tgagcagcaa cgcatggctg ttttgggcat tggcatcggc aggcttcgcc
    11341 tcattgaccg ccgttttcgc caaaatgggt ttacagggca tcgattccga tttcgccacc
    11401 tttatccgca ccttggtgat cctcgccgct ttggtattgt ttttgaccta caccggcaaa
    11461 tggcagggtg tgaacggctt tacggggcgc aactggacat tccttatcct atccggcctt
    11521 gctaccggcg catcctggct cgcctacttc aaagccctgc aactgggcaa cgcctcgcaa
    11581 gtcgccccca tcgacaaatt cagcctggtc ttggtcgcgc tgatggcggt ggttttcttg
    11641 gacgaacgcc cgaacacgca ggaatggata ggcttggggc tggtaacggc gggcgtgttg
    11701 gtgctggcgt tgaaacgtta aaccgaatcc gccataccgc ctgaaaccgg gttttgacaa
    11761 tctgacgggc ggcggtctga acaaagttac ccaataaaat ccgacaccgc gccgacggca
    11821 aaccgccgcg cttcagatgg catttgttgg acggaaagcc ccgaacgccc tattttcacg
    11881 cctccacgaa aatatgctgg acatccggtc ggaaccgttc ggcagccggc tcggcaagcc
    11941 accgttttat ttcacttcca agcccctgca agggcttgag cctctttcag acggcatacc
    12001 gtgccgacat ccagccacaa gcccgtatgc ttctgaccgc tcacgcggtt ttgccgcatt
    12061 tcgccacgca atacgggcgc gagtttcgcc acactgcccg cttcgattcc gtcaaacatt
    12121 tcaggacggt aaatacccac gccgctgaat gtcaatccgt tgccgccatt tacttccggc
    12181 cgcacgctgc cgtcgggcag cagggaaaaa tcgccgtcgg ggttgtgcgg cggattttcc
    12241 accagccaca gatgggcgga aatatgttcc ggcagggacg atgccgcctg aaacgcggcg
    12301 gtaaaatcga tgtcggtcag cacgtcgccg ttgaccacca aaaacggctg cccacccaac
    12361 agcggcaatg cctgcgcgat gccgcctgcc gtttccaagc cgcctgcggg ttcgggcgaa
    12421 taggcgatgt tcacgccata agccgagccg tcgcccaaag catcttctat ctgccgaccc
    12481 agccaagcgt ggttgatgac gatttcggta aaccccgcct gcttcagacg gcataggtgc
    12541 caaccgatta gaggcttacc cgccacatcg agcagcggct tcggagtggt atcggtcaaa
    12601 ggacgcatac gctcgccgcg tcctgccgcc agtatcatcg ctttcatata tatctgtccg
    12661 aatatcagtc taaagatcta aactgccgtc tgaaataaag cagcgcggga cgtttgccca
    12721 cgcaattttt aatttcgtcg agcctgacgt aaaacacaaa atgtgtgccg atttcatgtt
    12781 ggccgacaat atgcccgtgc agatgcgcca acgcgccttc tatttcaggt tgtccggttt
    12841 taccgtaatg ccaaatatga taggcaaaac gttcttcggg tgaaagattg gtcaatccgg
    12901 caaaatgttc ggcaacatcc tgatgttcgt ccgccagcat attgatgcag aggctgccgt
    12961 tttccgacag gatcggaatg attcgcgcac tccggttgat gcacagcatc acggtcggcg
    13021 gctcgtcggt aaccggcgca accgccgtca ttgtaatgcc gtaacgccct gccgcaccgt
    13081 ctgtcgtgat gacatgaacg cctgccgcgc aagatgccat cgcatcacgg aacgaagttt
    13141 gaaaagtttt ctgcaaatcc gccatttttc ccctttaaac tgtcccctat ataagaatgc
    13201 tgcacacaag gcacccccca tgtgcagcag ttctgattca aaaagccgtc ggtcggacgt
    13261 ttccgcgcgt tacggcgtat tacgagttca acgcatcctc gattttggca agttctgcca
    13321 acaggtcttt aagcagcagc attttctcgc ggcccagcac ttcctcgata gcgtcgtaac
    13381 gctcgtccac ttcttcgccg atttcctcat acagcttctc gccctcggca gtcagcttca
    13441 gaaaaacacg tcgttggtcg ttggaaggtt tcaggcggac aaccaaaccc gctttttcaa
    13501 ggcgggtcag gataccggtc aggctggggc gcagaataca cgcctgattc gccaaatctt
    13561 gaaagtccag cgtgccgttt tccgccaaaa gacggataat ccgccattgc tgatcggtaa
    13621 tattcgcctg attcagaata ggcctgaatt gggtcatcag ggcttccctt gcctgtatca
    13681 gactgatatt gatagacgca tgttttgatt gggtaggcat tgttcaagtc tccaagttat
    13741 cgaaaatcaa actttcaaac cgtcgggaaa gcctgtgggc gtaaattttg atgcaaccgt
    13801 tatataacaa aacgaacata tagcaacaat acgctataaa ccgcatcgga cgactgggta
    13861 taaaagactt taattccgat aatcctatct aaaaatattt taatagttat atcttagtct
    13921 attttcccta caatctcaac aaagtattac atttactgtc gaaagaactt tcttccagaa
    13981 aaaacaaaag ccgccgcatc agacgagcaa ggtatatgcc gtttgattct ctacatagcg
    14041 taaagtttaa caaaaacaaa agttaaccga aaacatccgc ctgaaaaatt cgtgcgcgca
    14101 agccccaata actgctgatt cccgtcgtat agtgaaccat tttcccattt ttgaccaaaa
    14161 cgacggcagg cgttgcgaca atccgccaag accttgccaa acccccgtcc tcatcgttga
    14221 cagtcggaaa gcccaagccc cgctttgcca tatacgccgc cacttcggac gcgctgccgg
    14281 aacgtaccgc cacgccgacg accggcacgc cgtccgccgc caaatcatcg attatcggcg
    14341 actgataacg gcacacgccg caccagctcc cccaaaaata caccaaaacc gccttatccc
    14401 ggctaaactg tcccaaagtc agccgctgcc ccgacagcag ggtcaaaggc cgccctgccg
    14461 caccggccgg ctcttcgggc ttgcgtatcc aatccaaaaa cagcgacacc aataaaaaca
    14521 ccaatgccgt ctgaacggca aatttgatgc ccgaaagcag tttctttttc atacgctctc
    14581 tcaaacggta cgcccgcgca accggcaggc aaacaaaaag ccaagtctca aaacttggct
    14641 tccggttatc tggtgggtcg tgagcgattc gaacgctcga ccaacggatt aaaagtccgc
    14701 tgctctaccg gctgagctaa cgacccgata agccgtgcat tatacagcac catcctacct
    14761 cgtcaagcaa attttacagg cttaattgca gaccactgtt tgcacgggat attttgacaa
    14821 cggattttca caatccgccg cataccgtgt aaaagttcgc acaaggaaag gcaaaccgcc
    14881 cgaaatcaat gtacactttc cgcccgtttc ccaaactgca cacagaaaca cacattatga
    14941 acatacaaaa catccgcacc ctcctcgaca ccgtcgccgt tccgaatacg gcacgcacgc
    15001 tcggcggcga aaaggccgtc cgttcggtcg aacagcgttc agacggcatc catatcgccc
    15061 tgcatttcgg cttccctgtc gcgcacatcg ccgccgccgt cgccgattcc gtacaagaaa
    15121 tcctgatgcc cgaaacaggc gacacacaca tccatctgtc catggacact gaaatcggca
    15181 cacacaaagt ccagcccggc gttaccacca tcaaaggcgt aaaaaacatc atcgccgtcg
    15241 catcgggtaa aggcggcgtg ggcaaatcga cgacgacagc caaccttgcc gccgcaatgg
    15301 cgcggatggg cgcgcgcgtc ggcgtactcg atgccgacct ttacggcccg agccagccca
    15361 cgatgctggg cgtacacgac cgcaaacccg atcagaaaaa ccaaaaactc attcccgtcg
    15421 aatcttcaga cggcatacag gttatgtcca tcggctttct cgtcgatacc gaccaagccg
    15481 tcgtctggcg cgggccgatg gtcagccaag ccttgcagca actgatgttt caaagcgagt
    15541 gggacgaagt ggattatctt ttcatcgacc tgccccccgg cacgggcgac atccaactca
    15601 cgctgtccca acgcatcccc gtaaccggat cggtcatcgt aaccacgccg caggacatcg
    15661 ccctgataga cgcgcgcaag gctgtggata tgttccgcaa ggtcaacatt cccattttag
    15721 gcgtattgga aaacatgtcc gtccacatct gctccaactg cgggcatagc gaagcactgt
    15781 tcggcacgga cggcggcaaa gatttggcgg cccgcctcaa cgtccccctg ctcggacagc
    15841 ttcccctaag cctgcccgtg cgcgaagcca tggacggcgg cacaccggcg caactgttcg
    15901 acgaacaccc cgccatcgcc cgaatctaca ccgatgccgc attccaaatc gccctgagca
    15961 ttgccgacaa aggcaaagac ttcagcagcc gcttccccaa aatcgtcgtc gaataaagcc
    16021 gcgtccgaaa ccgcaacagc aatgccgtcc caagccccgc gcctgccggc gggcaaactt
    16081 gccggataaa acggtttttt tgagatttta cgttccggat tcccactttc gtgggaatga
    16141 cgg
//
LOCUS       DAWFAD010000037        16044 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWFAD010000037 DAWFAD010000000
VERSION     DAWFAD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16044
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.037"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            299..1627
                     /gene="miaB"
                     /locus_tag="WGO49_000866"
     CDS             299..1627
                     /gene="miaB"
                     /locus_tag="WGO49_000866"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ0305993.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1712..3625)
                     /gene="dxs"
                     /locus_tag="WGO49_000867"
     CDS             complement(1712..3625)
                     /gene="dxs"
                     /locus_tag="WGO49_000867"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ0305994.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAVADKQLGNNRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3710..4627)
                     /gene="xerC"
                     /locus_tag="WGO49_000868"
     CDS             complement(3710..4627)
                     /gene="xerC"
                     /locus_tag="WGO49_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ0305995.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDVGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRRDE"
     gene            4821..5885
                     /locus_tag="WGO49_000869"
     CDS             4821..5885
                     /locus_tag="WGO49_000869"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ0305996.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAAGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     ISLEKMATKYAKGELNQIVK"
     gene            6044..6865
                     /locus_tag="WGO49_000870"
     CDS             6044..6865
                     /locus_tag="WGO49_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ0305997.1"
                     /translation="MNRTAFCCFSLTAALILTACSSGGGGVAADIGAGLADALTAPLD
                     HKDKGLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIE
                     VDGQLITLESGEFQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAF
                     NQLPSGKAEYHGKAFSSDDPNGRLHYSIDFTKKQGYGRIEHLKTPEQNVELASAELKA
                     DEKSHAVILGDTRYGGEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            7003..7680
                     /gene="tsaB"
                     /locus_tag="WGO49_000871"
     CDS             7003..7680
                     /gene="tsaB"
                     /locus_tag="WGO49_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ0305998.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETRLFHQEVGSRQSALI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFDTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHAELLYVRNKIALTAKEQ
                     AERRARL"
     gene            7677..8117
                     /gene="rimI"
                     /locus_tag="WGO49_000872"
     CDS             7677..8117
                     /gene="rimI"
                     /locus_tag="WGO49_000872"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ0305999.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQRQFESALVSLSEQVFL
                     AEKDGGLAAFIVWQNLPDESELHLIATAPECRRQGIASALLEYWFAHLPEGTQRLLLE
                     VRAGNTAAQALYAAHGFSIAGRRKNYYRATDGQTEDAVLMEKIC"
     gene            8111..8845
                     /locus_tag="WGO49_000873"
     CDS             8111..8845
                     /locus_tag="WGO49_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ0306000.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVPTRKPAPETETPLPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGNPMP
                     SEQAVANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARNWQVLKQLKHALQAGGS"
     gene            8907..9548
                     /gene="pyrE"
                     /locus_tag="WGO49_000874"
     CDS             8907..9548
                     /gene="pyrE"
                     /locus_tag="WGO49_000874"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ0306001.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKARRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9622..10056
                     /locus_tag="WGO49_000875"
     CDS             9622..10056
                     /locus_tag="WGO49_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306002.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTLHDTPPATAATP
                     AAAPQVAVPPAAPARQEGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10053..11363
                     /gene="argA"
                     /locus_tag="WGO49_000876"
     CDS             10053..11363
                     /gene="argA"
                     /locus_tag="WGO49_000876"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ0306003.1"
                     /translation="MIVPDLFVAHFREAVPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFYLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AETLSAQEAQSLAEHAGGETRRLISSTVAALEGGVHRVQILNGASDGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEISCLAVSPQAQDGGYGERLLAHIIDKARSMGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11459..13474
                     /locus_tag="WGO49_000877"
     CDS             11459..13474
                     /locus_tag="WGO49_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ0306004.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILNQMQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEKPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNQVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIDKDAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPNDCDVVGYLAGHLLL
                     TLRKDWNRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFDSDGIEVRQFWVMSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPQHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQREAADELACVLLFLKEFLG"
     gene            complement(13588..14544)
                     /locus_tag="WGO49_000878"
     CDS             complement(13588..14544)
                     /locus_tag="WGO49_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0306005.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRSFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     ALIESGSTVQAAMHFCGYRHAGRFNEAFRRHYGFLPSDVKKC"
     gene            14780..15745
                     /locus_tag="WGO49_000879"
     CDS             14780..15745
                     /locus_tag="WGO49_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0306006.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT         3568 a         4871 c         4395 g         3210 t
ORIGIN      
        1 tgttaatcca ctataacgca ggaactgatg ttccctgtcg ccgaaattgc tggtacacgc
       61 acacagcagc aatgccgccc acacagccgg tttcatacac acctcccatt aaagccaaac
      121 attatacagc cgttctgacc gattaaattc atattttaaa acaatattct gtttacaaaa
      181 cccacatcgt gctataatcc gcaccgattt tcagacggca tcgtcatgcc gtctgaaatt
      241 ttttcattcc aacaaccatc agccccgcga ttacggctgc ctgagaaaga cacaaaccat
      301 gaaaaaagta tttatccgta ccttcggctg ccagatgaac gaatacgaca gcgacaaaat
      361 gctcgccgtc ctcgccgaag aacacggcgg catcgaacag gttacccaag ccgacgaagc
      421 cgacatcatc ttgttcaaca cctgctccgt gcgcgaaaaa gcacaggaaa aagtgttctc
      481 cgatttgggg cgcgtgcgtc cgctcaaaga aaaaaacccc ggcctcatca tcggcgttgc
      541 cggctgcgtc gcctcgcaag aaggcgaaaa catcatcaaa cgcgcgcctt atgttgacgt
      601 ggtcttcggc ccgcaaacgc tgcaccgcct gcccaaaatg attgtggaca aagaaaccag
      661 cgggctgtcg caagtcgata tttccttccc cgaaatcgaa aaattcgacc acctgccgcc
      721 cgcccgcgtc gaaggcggcg cggcatttgt atcgattatg gaaggctgtt ccaaatactg
      781 ctcattctgc gtcgtcccct acacgcgcgg cgaagaattc tcccgcccgc tcaacgacgt
      841 attgacagaa atcgccaacc ttgcccagca aggcgtgaaa gaaatcaacc tcttgggaca
      901 aaacgtcaac gcctatcgcg gcgaaatgga cgacggcgaa atctgcgact tcgccaccct
      961 gctgcgcatc gtccacgaaa tccccggcat cgaacgtatg cgcttcacca ccagccaccc
     1021 gcgcgagttt accgactcga ttatcgagtg ctaccgcgac ctgcccaaat tggtttccca
     1081 cctgcacctg ccgattcaaa gcggttccga ccgcgtattg agcgcgatga aacgcggcta
     1141 caccgctttg gaatacaaat ccatcatccg caaactgcgt gccatccgtc ctgatttatg
     1201 cctgagcagc gatttcatcg tcggcttccc cggcgagacc gaacgcgagt tcgagcaaac
     1261 cttgaaactg gtgaaagaca ttgccttcga cttgagcttc gtgtttattt acagtccgcg
     1321 ccccggcacg cctgccgcca acctgcacga cgacacgccg cacgaagaaa aagtgcgccg
     1381 cctcgaagcc ttgaacgaag tcatcgaagc cgaaaccgcg cgcatcaacc aaaccatgat
     1441 cggcacggtt caacgctgcc tggtcgaagg catctccaaa aaagaccccg accaactgca
     1501 agcccgtacc gccaacaacc gcgtcgtcaa cttcaccggc acgcccgaca tgattaacca
     1561 aatgatcgat ttggaaatca ccgaggccta caccttctcc ctgcgcggca aagttgtcga
     1621 agcctaaacc ctcacgccga aaaaatgccg tctgaagcgt ttcagacggc attttgcctt
     1681 gtatcggcag acgacggcgc ggccgggcgg cttaatttgc cgcatcccga tccgacagcc
     1741 acgcgcgcac acgccgttcc accgcttcgg cactcaagcc caaatcgtct aaaagttttt
     1801 tcggatcgcc gtgtccggtt acggtatcgg caacgcccaa aagcaaaacg ggtttgcaga
     1861 taccgtgttt cgccaatact tccagcaccg cgccgcctgc gccgccctgt tcggcgtttt
     1921 cttcaagggt aacgatgcgg tcgtggcttc gggcaaggcg gacaatcaac tcttcgtcta
     1981 tcggtttgac aaagcgcata tcggcgacgg tggcgttcag ttttccggcg accgccaatg
     2041 cgggggcgac catactgccg aaggcaatga atgcggtttt cccaccttcg cggcggataa
     2101 tgcccttgcc gatttccacg gtttccatgc cgtctgaaac cggcgcgccc gtacccgtgc
     2161 cgcgcggata gcggacggcg gcgggcgcat ccgcctgata gcaggtcgaa agcagcaggc
     2221 ggcattcgtt ttcatcgctc ggcgcggcga caatcatatt cggaatgcaa cgcaagaagc
     2281 tcaaatcgta caaaccggcg tgggtcgggc cgtccgcgcc gacgatgccc gcgcggtcga
     2341 cggcaaacaa aacgggtagg ttttgcaggg cgatatcgtg caccagttgg tcgtaggcgc
     2401 gttgtaaaaa ggtggaataa atcgccacga cgggcttcat gccttcgcac gccaaaccgc
     2461 cggcaaaggt aacggcgtgc tgctcggcaa taccgacatc gaaataacgg tcggggaatc
     2521 gttgttcaaa ctccaccagt ccgctgccct cgcgcatcgc gggcgtgatg gcgatcaaac
     2581 gctcgtctgc cgccgcccgg tcgcacagcc atttgccgaa cacttgggta taggtcggtt
     2641 tggcgacggg cttgggttct ttttcagacg gcatttgcgc cgcgccttct ttaggcagct
     2701 tggcgacggc gtggtatttg acggggtcgt tttcggcgag tttgtagccg ttgccttttt
     2761 tggtgatgac gtgcagaagc tgagggcctt tgcggctgcg caagtctttc aatacgtcca
     2821 ccagattttc gacgttgtgt ccgtccacgg ggccggtata gcggaagccg aagttttcaa
     2881 acaaagacag cgactgtttg gcgtgttcgg cttcttcggc aagggttttg attttgtgtt
     2941 cgactttttg ggcaaactcc atcgcgccgg gtattttgtc taataccttg cccgtttgcg
     3001 ctttgacggt actcaacagg ccgtgcatat cgcgcacgac gttgctggca aggtatttcg
     3061 gcagcgcgcc gacgttgggg gaaatcgaca tttcgttgtc gttgaggacg accagcaaat
     3121 ccacatccat atcgcctgcg cagttcaggg cttcaaacgc ctgaccagcc gtcatggctc
     3181 cgtcgccgat gatggcgacg ctgcggcggt tgttgcccaa ctgtttgtcc gcaacggcca
     3241 tgcccaaggc cgcgccgatg gatgtggaag aatgccctac gccgaacgcg tcgtattccg
     3301 actcgctgcg ttttgggaag ccagctaaac cgccgtattg gcgcatggtg tgcatctggt
     3361 ttttcctgcc tgtcaggatt ttgtgcggat agctttggtg tccgacatcc cacaccagct
     3421 tgtcttcggg cgtgtcgtac acatagtgca gggcgatggt cagttcgacc gcgcccagat
     3481 tgctggcgaa atgcccgccg gtctgcccga cagattccag cagaaaggca cgcaactcgc
     3541 cggcaaggcg cggcagctgt tttttgtcca gacggcgcaa atcttgcggg ctgtcaatca
     3601 ggtcgagtag ggggcttggg ttcatggtgt gtctttttta tgtgtcgtcc gggtgcaacg
     3661 gtcaattata tatcaagagc gtgcggctga cggctgattt tgccgtatgt cattcgtccc
     3721 gccgcttggc gcgcgggtgg gcttcgtcat acaggcgggc gatgtggtcg aaatcgagtt
     3781 tggtgtaaat ctgcgtggtc gaaaggctgc tgtgtccgag cagctcctgc accgcccgga
     3841 tgtcgcgcga agcctgcaac aggtgtccgg catagctgtg gcgcatcata tgcggcgaaa
     3901 cgtgtctgcc gtctccgttt tgcgccgccc attgcgccaa acgtttttgg atttggcgtt
     3961 ggctcaggcg cgtgccgttc ctgccggtaa acagggcttt gccgtccgat gccgtctgac
     4021 gcagcggcag atagtttttc agggcttcca cgcttttgcc ggtcagcggc acctgccgct
     4081 gcttgcgccc tttgccggta acgtgtaccc acgcctcgtc caaatagaca tcatctgcat
     4141 tcaagccgtg tatctcgctc acgcgcaaac cgctgccgta catcagctcg aacagggcgt
     4201 gatcgcgcac cgccagcggg tcgccgccgt ccacgggcaa atccagcatc cggttcagcc
     4261 attcctgcgg cagggctttg ggtacgcgct cgggctgctt cggcggtttg atgtcggcgg
     4321 tcgggtcggt atgcagcaga ccgcgttcca ccagccaaac acaatactgc cgccaagacg
     4381 acagcttgcg cgccagcgtc cgcgcattca aaccgcgctg cgacagccgc cgcaacgccg
     4441 ccgtaaaatc gcgccgcgac aagtcctgcg gcacgccgcc tacatcttca gacggcattt
     4501 gtgccaacag cgcaaacagt tcttccaaat cgcgccggta tgccgccacc gtgtgctccg
     4561 acttgccctc gcgcacgatg ttttccaaat aagcgtcaaa atgcgccgca aacccgtcca
     4621 aaaccataac cgccccacac aaatatcaaa aaaacagtga aaacattatc ataaacgaca
     4681 caccaaaacc ccgaaccgcc caaaatccga cccgtccgcc cgccgttatc gttaacataa
     4741 aatcaatgag ccgtttatgg ttttttgccg caaaaaacat tataatccgc cttatttacc
     4801 tattgcccaa ggagacacaa atggcactcg tatccatgcg ccaactgctt gatcatgctg
     4861 ccgaaaacag ctacggcctg cccgcgttca acgtcaacaa cctcgaacaa atgcgcgcca
     4921 ttatggaagc cgccgaccaa gtcaacgcgc ccgtcatcgt acaggcgagt gcaggtgcgc
     4981 gcaaatatgc gggtgcgccg tttttacgcc acctgattct ggcggctgtc gaggaatttc
     5041 cacacatccc cgtcgtcatg caccaagacc acggcgcatc gcccgacgtg tgccaacgct
     5101 ccatccaact gggcttctcc tccgtgatga tggacggttc gctgatggaa gacggcaaaa
     5161 ccccttcttc ttacgaatac aacgtcaacg ccacacgtac cgtggttaac ttctcccacg
     5221 cttgcggcgt atccgtcgaa ggcgaaatcg gcgtattggg caacctcgaa accggcgaag
     5281 caggcgaaga agacggcgtg ggcgcggcag gcaaactttc ccacgaccaa atgctcacta
     5341 gcgttgaaga tgccgtacgt ttcgttaaag ataccggcgt tgacgcattg gctattgccg
     5401 tcggcaccag ccacggcgcg tacaaattca cccgtccgcc cacaggcgac gtattgcgta
     5461 tcgaccgcat caaagaaatc caccaagccc tgcccaatac acacatcgtg atgcacggct
     5521 ccagctccgt tccgcaagaa tggctgaaag tcatcaacga atacggcggc aatatcggcg
     5581 aaacctacgg cgtgccggtt gaagaaatcg tcgaaggcat caaacacggc gtgcgcaaag
     5641 tcaacatcga caccgacttg cgccttgcgt ccaccggcgc ggtacgccgc taccttgccg
     5701 aaaacccgtc cgactttgac ccacgcaaat acctgagcaa aaccattgag gctatgaagc
     5761 aaatctgcct cgaccgttat cttgcgttcg gttgcgaagg tcaggcaggc aaaatcaaac
     5821 cgatttcttt ggaaaaaatg gcaaccaaat acgccaaagg cgaattgaac caaatcgtca
     5881 aataacaggt tgcctgtaaa caaaatgccg tctgaaccgc cgttcggacg gcatttgatt
     5941 tttgcttctt tgacctgcct cattgatgcg gtatgtaaaa aaagatacaa taactagggt
     6001 gtttatatat tatctatatc tgcgtatgac tgggagtaaa cctgtgaacc gaactgcctt
     6061 ctgctgcttt tctctgaccg ccgccctgat tctgaccgcc tgcagcagcg gagggggcgg
     6121 tgtcgccgcc gacatcggtg cggggcttgc cgatgcacta accgcaccgc tcgaccataa
     6181 agacaaaggt ttgcagtctt taacgctgga tcagtccgtc aggaaaaacg agaaactgaa
     6241 gctggcggca caaggtgcgg aaaaaactta tggaaacggc gacagcctca atacgggcaa
     6301 attgaagaac gacaaggtca gccgcttcga ctttatccgt caaatcgaag tggacgggca
     6361 gctcattacc ttggagagcg gagagttcca aatatacaaa caggaccact ccgccgtcgt
     6421 tgccctacag attgaaaaaa tcaacaaccc cgacaaaatc gacagcctga taaaccaacg
     6481 ctccttcctt gtcagcggtt tgggtggaga acataccgcc ttcaaccaac tgcccagcgg
     6541 caaagccgag tatcacggca aagcattcag ctccgacgac ccgaacggca ggctgcacta
     6601 ctccattgat tttaccaaaa aacagggtta cggcagaatc gaacacctga aaacgcccga
     6661 gcagaatgtc gagcttgcct ccgccgaact caaagcagat gaaaaatcac acgccgtcat
     6721 tttgggcgac acgcgctacg gcggcgaaga aaaaggcact taccacctcg cccttttcgg
     6781 cgaccgcgcc caagaaatcg ccggctcggc aaccgtgaag ataagggaaa aggttcacga
     6841 aatcggcatc gccggcaaac agtagcattt gaaaaatgcc gtccgaacac aataatttac
     6901 cgttcggacg gcatttttgc gccataccgc cctaggacac gacggcacgg caggcaagcc
     6961 gatttcacta tataatgccg tctgaaccaa cgagagaatg ccatgcaagc tgatttcaac
     7021 cgccccgtcc tcgccgtcga taccggaact tcctatttgt cgctcgcgct gcgcgccgac
     7081 ggcgaaaccc gtctgttcca tcaggaagtc ggcagccgcc agtccgcact gatcctgccg
     7141 gaaatccgca ccctattccg aaatgcaggc attaccgccg ccgatttggg ggcgattgtt
     7201 tatgcgaaag gccccggcgc gtttacggga ctgcgtatcg gtatcggcgt ggcgcaaggt
     7261 ttggcaacgc cgtttgatac gcctttaatc ggcatcccca cgctcgatgc cgccgcctcg
     7321 ctgccgccgc cgcaaagctg catactcgcc gctgcggacg cgcgtatggg cgaagtgttt
     7381 tatgcgtggt tcgacacgct gaaccgccgc cgtttgagcg attatcaggt cgggcgggcg
     7441 gcagacatcc ggctgccgga gggatgtgcc ttttcagacg gcataggcag cgcgttcgcg
     7501 ttggaagaaa cgccgccgtt ttcaggcaaa cccgatatgc cgaccgccgc cgacttcctc
     7561 aatctggcgg ctgaaggcgg ttttcccgcc gtcgctgcgg cacacgccga gctgctctac
     7621 gtccgcaaca aaatcgccct gactgccaaa gaacaggcgg aacggagggc gcgcctgtga
     7681 acatccgccg cgccgtttat gccgattgcg cggcactcgc cgcactcgat gccgtctgca
     7741 acccgtccgc gtggacgcaa cgccaatttg agtctgcatt ggtttcgctg tccgaacagg
     7801 ttttcctcgc ggaaaaagac ggcgggttgg ccgcctttat cgtttggcag aacctgcccg
     7861 acgaatccga actgcacctg attgccaccg cgcccgaatg ccgccgccaa ggaattgcgt
     7921 ccgccctgct cgaatattgg ttcgcacatc tgcccgaagg cacgcaacgc ctgctgctcg
     7981 aagtccgcgc aggcaacacc gccgcgcagg cactgtacgc ggcgcacggc ttcagcattg
     8041 cgggcaggcg gaaaaactat taccgcgcca ccgacgggca aaccgaagat gccgtcttaa
     8101 tggagaaaat atgttaagcg cgcgctacct ccacctgcac gaagctttgg gtttgggtcc
     8161 gatgtggctg aaacaggccg ctgccgtcct gccgcccaaa aacacacccg caaccccggc
     8221 acaggcacgt cctcaaaccg tccgcgccgc cccgatccgc ccttcccaac cccataacgg
     8281 tcaggcgcgg ctcgaaacga tgaaagcgtt ggaaaccgcc gccgtaccta cgcgcaaacc
     8341 cgcgcctgaa accgaaacgc ctctgcccgg cctttcagac ggcatcgccc ccgttcccgc
     8401 cgcttcgggc atcaccaagc ttgccgtcgt cagcctttgc ccaccgatcg aggatgcggt
     8461 ttacgggcaa ctgttccacg gcaaagcggg tgtcctgctt gacaacatac tcaaagccgt
     8521 aggactggat gccgcttatg tacacaagac cagctgggtg aaaaccgccg ccgtcggcaa
     8581 cccgatgccg tctgaacagg ccgtcgcgaa tgcgctgggt caaatcgcca gggaactcga
     8641 cggctgccgt gcccccgccg tccttttcct cgggcaggct tttgtccagc ctgaacggca
     8701 aacgatgatt gaaactttgt gcggcagccg tcccttcttc atcatcgacc atcccgctcg
     8761 gcttttacgc caacccgaac ttaaagcccg aaactggcag gtgttgaaac agttgaaaca
     8821 cgccttgcag gcaggcggca gttgaagcag gccgcacggg gcggtagaat cgcaactgcg
     8881 tcccaatatc tgacagaaag cacaaaatga ccgatttccg ccaagatttc ctcaaattct
     8941 ccctcgccca aaatgttttg aaattcggcg aattcaccac caaagccaga cggcggtcgc
     9001 cctatttctt caatgccggc ctctttaacg acggcttgtc cacgctgcaa ctggcaaaat
     9061 tctatgcaca atccatcatt gaaagcggca tccgattcga tatgctgttc ggtcccgcct
     9121 acaaaggtat tattttggcg gcggcaaccg cgatgatgct ggcggaaaaa ggcgtgaacg
     9181 tcccgtttgc ctacaaccgc aaagaagcca aagaccacgg cgaaggcggc gtgttggtcg
     9241 gcgcgccgct taaagggcgc gtgctgatta tcgacgacgt gatttccgcc ggcacatccg
     9301 tacgcgaatc gatcaaactg attgaagcgg agggtgcaac ccccgccggt gtcgccatcg
     9361 cgctcgaccg catggaaaaa ggcacgggtg aattgagcgc ggttcaggaa gtggaaaaac
     9421 aatacggtct gcccgtcgcc cccatcgcca gcctgaacga tttgtttatt ctgttgcaaa
     9481 acaaccccga attcggacag ttcctcgaac ccgtccgagc ctaccgtcgg cagtacggcg
     9541 tagaataaaa acaaagcata tgccgtccga accgccttac gcctcagacg gcatcaaacc
     9601 tgacacacac gaggaaatac catgcccgcc tgtttctgcc cccactgcaa aacccgtctc
     9661 tgggtcaaag aaacccaact caatgtcgcc caaggcttcg tcgtctgcca aaaatgcgaa
     9721 ggactgttta aagccaaaga ccatctggca agcacgaaag aacccatatt caacgatttg
     9781 cccgaggctg tttcggatgt caaactcgtt caccgcatcg gcacgcgcgc cattggcaag
     9841 aaacagattt cccgcgacga aatcgccggc atcctcaacg gcggtacaac cctgcacgat
     9901 acgccgcccg caaccgccgc cacccctgcc gccgcaccgc aggttgccgt accgcccgcc
     9961 gcgcctgccc gtcaggaagg gttcaactgg acgattgcaa ccctgtttgc ccttatcgtc
    10021 ctcattatgc agctttccta cctcgtcatc ctatgatcgt gcccgacctc tttgtcgccc
    10081 acttccgcga agccgtcccc tacatccgcc aaatgcgcgg caaaacgctg gtcgccggca
    10141 tagacgaccg cctgctcgaa ggtgatacct taaacaagct cgccgccgac atcgggctgt
    10201 tgtcgcaact gggcatcagg ctcgtcctca tccacggcgc gcgccacttc ctcgaccgcc
    10261 acgccgccgc gcaaggccgc acgccgcatt attgccgggg cttgcgcgtt accgacgaaa
    10321 cctcgctcga acaggcgcag cagtttgccg gcaccgtccg cagccgtttt gaagccgcat
    10381 tgtgcggcag cgtttccggg ttcgcgcgcg cgccttccgt cccgctcgta tcgggcaact
    10441 tcctgaccgc ccgtccgata ggtgtgattg acggaaccga tatggaatac gcgggcgtta
    10501 tccgcaaaac cgacaccgcc gccctccgtt tccaactcga cgcgggcaat atcgtctggc
    10561 tgccgccgct cggacattcc tacagcggca agaccttcta tctcgatatg cttcaaaccg
    10621 ccgcctccgt cgccgtctcg cttcaggccg aaaaactcgt ttacctgacc ctttcagacg
    10681 gcatttcccg ccccgacggc acgctcgccg aaaccctctc ggcacaggaa gcgcaatcgc
    10741 tggcggaaca cgccggcggc gaaacgcgac ggctgatttc gtccaccgtt gccgcgctcg
    10801 aaggcggcgt gcatcgcgtc caaatcctca acggtgcctc cgacggcagc ctgctgcaag
    10861 aactcttcac ccgcaacggc atcggcacat ccattgccaa agaagccttc gtctccatcc
    10921 ggcaggcgca cagcggcgac atcccgcaca tcgccgccct catccgcccg ctggaagaac
    10981 agggcatcct gctgcaccgc agccgcgaat acctcgaaaa ccacatttcc gaattttcca
    11041 tcctcgaaca cgacggcaac ctgtacggtt gcgccgccct gaaaaccttt gccgaagccg
    11101 attgcggcga aatctcctgc cttgccgtct cgccgcaggc acaggacggc ggctacggcg
    11161 aacgcctgct tgcccacatt atcgataagg cgcgcagtat gggcataagc aggctgttcg
    11221 ccctctccac aaataccggc gaatggtttg ccgaacgcgg ctttcagacg gcatcggaag
    11281 acgagttgcc cgaaacgcgg cgcaaagact accgcagcaa cggacgaaac tcgcatattc
    11341 tggtacgtcg cctgcaccgc tgaccgcaac ggaaagccgc cgcagaaatg ccgtctgaac
    11401 cccgtttcag acggcatttc cccgattata aagacctgcc caaccctcaa ggaccccgat
    11461 gaaatcctac cccgacccct accgccattt tgaaaacctc gattccgccg aaacgcaaaa
    11521 cttcgctgct gaagcgaatg ccgaaacgcg cgcgcgtttt ttaaacaacg acaaggcgcg
    11581 cgcgctttca gacggcattt tgaatcaaat gcaggacacg cggcagattc cgttttgtca
    11641 ggaacaccgc gcgcggatgt accatttcca tcaggacgcg gaatatccga agggcgtgta
    11701 ccgcgtgtgc acggcggcga cctaccgttc gggctatccg gagtggaaaa tcctgttttc
    11761 agtggcggac ttcgacgaat tgctcggcga cgatgtatat ttgggcggcg tgtcgcactt
    11821 ggtggaaaag cccaaccgcg cgttgttgac actaagcaaa tcaggcggcg atacggcgta
    11881 cacgctggaa gtggatttgg aagcagggga attggtagaa ggtggttttc actttccggc
    11941 aggcaaaaac caagtgtcgt ggcgcgatga aaacagcgtg tgggtgtgtc cggcttggga
    12001 cgaacgccag ttgaccgaat cgggctatcc gcgtgaggtg tggctggtgg aacgcggcaa
    12061 gagtttcgag gaaagcctgc ctgtgtatca aatcgataaa gatgcaatga tggtgaacgc
    12121 gtggcgttat ctcgatccgc agggttcgcc gattgatttg attgaagcgt cggacggttt
    12181 ttacaccaaa acctatttgc aggtgtcggc cgaaggcgag gcgaaaccgt taaacctgcc
    12241 caacgattgc gatgtggtcg gctatctggc ggggcatctt ttgctgacgc tgcgcaagga
    12301 ctggaaccgc gcgaaccaaa gctatccgag cggtgcgctg gtggcggtga agctgaatcg
    12361 gggtgaactc ggggcggcgc agcttttgtt tgcgcctgat gaaacgcagg cattggaaag
    12421 cgtggaaacg accaagcgtt ttgtggtggc gagcctgctg gaaaacgtac aaggccgtct
    12481 gaaagcgtgg cgttttgccg acggcaaatg gcaggaagtc gaactgccgc gcctgccttc
    12541 gggcgcgttg gaaatgaccg accagccttg gggcggcgac gtggtttacc ttgccgccgg
    12601 cgatttcacc acaccgctga cgctgtttgc gctggatttg aacgtgatgg aactgaccgt
    12661 catgcgccgc cagccgcagc agttcgattc agacggcatc gaagtgcggc agttttgggt
    12721 gatgtcgtcc gacggcgaac gcatccctta tttccacgtc ggcaaaaacg ccgcgcccga
    12781 cacgccgacg ctggtttatg cctacggcgg tttcggcgta cccgagctgc cgcattatct
    12841 gggcagcatc ggcaaatact ggctggaaga gggcaacgcc ttcgtgttgg cgaacatacg
    12901 cggcggcggc gagttcggcc cgcgctggca tcaggcggcg cagggaatca gcaaacacaa
    12961 aagcgtcgat gatttgcttg ccgtcgtgcg cgatttgtcc gagcgcggca tcagttcgcc
    13021 ccaacacatc ggtttgcagg gcggcagcaa cggcggcctg attaccgccg ccgccttcgt
    13081 gcgcgaaccg caaagcatag gcgcactggt gtgcgaagtg ccgctgaccg acatgatccg
    13141 ttatccgctg ctctccgccg gttcaagctg gacagacgaa tacggcaatc cgcaaaaata
    13201 cgaagtctgc aaacgccggt tgggcgaatt gtcgccgtat cacaatcttt cagacggcat
    13261 cgattatccg cccgcgctca tcaccaccag cctcagcgac gaccgcgtcc atcccgccca
    13321 cgcgctcaag ttttacgcca aactgcgcga aacctcgccg caatcttggc tctactcgcc
    13381 tgacggcggc ggccataccg gcaacggcac gcagcgcgaa gccgccgacg aactcgcctg
    13441 cgtgttgctg tttttgaaag agtttttggg ctaagggcgg ggagcggcac tgccgccgcg
    13501 aatgaaaaaa ggtcgtctga aactgctttt tcagacgacc ttttttaatg gttgtttcaa
    13561 atcaaaatat ctatgccgcc ggccccatca gcacttcttc acatccgaag gcaaaaatcc
    13621 gtaatgccgt ctgaacgctt cgttgaaccg tcccgcgtgg cggtagccgc aaaagtgcat
    13681 ggcggcttgg acggtgctgc cggattcgat gagggcgagc gcgtgttcca gccgcaggcg
    13741 gcgcaggcat ccggcgacgg tttcgccggt ttgcgctttg aaatagcgtt tcaggtagca
    13801 ttcgttcagt ccgacgcggc gggcgatttc ggcgatggtc agcggacggg cgaattcgtg
    13861 ttgcaggatg tcggcggctt cgtctatgcg ccgacggcgg taaccgttgt cgtggcggcg
    13921 gaaggtgaag cgcaataatc gggcggagag ttccagcgcg gcggcttcgt cggcaagcag
    13981 gccgaagccg tccgattcga acgagcgttg cagcagtggg caggccgccg ccgtcagtgc
    14041 cgctgcgttt tgcgccagcc gttgcagggc gaatcggcct atcgtttgcg gcgaaaacag
    14101 gcgttcgtcc agcaagcctt cgtcgtgcca gcggcgcagt ttttccagcg aaaaatccaa
    14161 atgcagcgcg cacatgccgc tgttgtcggg cagcagggtt tcggatacgt ccgccaactc
    14221 gccgcgtacc agccagattt cgccggcaga cgggcggtat tccctgccgc ccatttgtaa
    14281 ccggttctgc cccgacacca tgacgaacaa ggcgcagttg tggctgaaat tgtggatttc
    14341 ggtgggaaac gcgcccgttc cgccgccgcg catccgcgac aaggtgatgc ccgagtcgaa
    14401 gcggttgatg cacatttcca gatgcaaacc gggctgtttt gcctgcgcaa tgagcgcgct
    14461 gtcggaacag ccgtccaacg cccagccgga tttatcggag cggacatagg tttggtactg
    14521 gcggtagatg gcggcggtgt tcatgattgg ataggaacga gttgtctaac aaatgaatta
    14581 aataggaatt attaccaata atcaagcgca gggattggtt gaaacggaaa aggtcgtctg
    14641 aaagggtgtt tcagacgacc ttttccgtat cgggaatttg ttttgccgta tcgggaattt
    14701 tgcgttttgc ggcgtggttt ctgcaggttg tttgcttaat aataaacatt cttattcgta
    14761 tgcaaaggaa ccgcacaccg tgaaaccgcg tttttattgg gcagcctgcg ccgtcctgct
    14821 gaccgcctgt tcgcccgaac ctgccgccga aaaaactgta tccgccgcat ccgcatctgc
    14881 cgccacactg accgtgccga ccgcgcgggg cgatgccgtt gtgccgaaga atcccgaacg
    14941 cgtcgccgtg tacgactggg cggcgttgga tacgctgacc gaattgggcg tgaatgtggg
    15001 cgcaaccacc gcgccggtgc gcgtggatta tttgcagcct gcatttgaca aggcggcaac
    15061 ggtggggacg ctgttcgagc ccgattacga agccctgcac cgctacaatc ctcagcttgt
    15121 cattaccggc gggccgggcg cggaagcata tgaacagttg gcgaaaaacg cgaccaccat
    15181 agatctgacg gtggacaacg gcaatatccg caccagcggc gaaaagcaga tggagacctt
    15241 ggcgcggatt ttcggcaagg aagcgcgcgc ggcggaattg aaggcgcaga ttgacgcgct
    15301 gttcgcccaa acgcgcgaag ccgccaaagg caaaggacgc gggctggtgc tgtcggttac
    15361 gggtaacaag gtgtccgcct tcggcacgca gtcgcggttg gcaagttgga tacacggcga
    15421 catcggccta ccgcctgtag acgaatcttt acgcaacgag gggcacgggc agcctgtttc
    15481 cttcgaatac atcaaagaga aaaaccccga ttggattttc atcatcgacc gtaccgccgc
    15541 catcgggcag gaagggccgg cggctgtcga agtattggat aacgcgctgg tacgcggcac
    15601 gaacgcttgg aagcgcaagc aaatcatcgt catgcctgcc gcgaactaca ttgtcgcggg
    15661 cggctcgcgg cagttgattc aggcggcgga gcagttgaag gaggcgtttg aaaaggcaga
    15721 acccgttgcg gcggggaaag agtagggagt cgtctgaaaa cggagtttcc gaaggaagcg
    15781 gggggtttct gcgaagctaa agtgcggttt caacgaattg aaaagcagcc tgtatgttga
    15841 aaataccgct caagcaaacc tacggtttgc cgccctctcc ctagccctct cccacaggga
    15901 gaggggattg gggtgcaggc tgcctttaag gtttaggcaa atttttaact tcgttgaagc
    15961 tgcgatttca gaagctccgt tttagcttcg cagaaactcc gcttccttcg aaagctccgt
    16021 tttcagacga ccttttggag tacc
//
LOCUS       DAWFAD010000038        15528 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWFAD010000038 DAWFAD010000000
VERSION     DAWFAD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.038"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            6..81
                     /locus_tag="WGO49_000880"
     tRNA            6..81
                     /locus_tag="WGO49_000880"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Trp,seq:cca)
     gene            196..474
                     /gene="secE"
                     /locus_tag="WGO49_000881"
     CDS             196..474
                     /gene="secE"
                     /locus_tag="WGO49_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ0306007.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            476..1012
                     /gene="nusG"
                     /locus_tag="WGO49_000882"
     CDS             476..1012
                     /gene="nusG"
                     /locus_tag="WGO49_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ0306008.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1113..1547
                     /gene="rplK"
                     /locus_tag="WGO49_000883"
     CDS             1113..1547
                     /gene="rplK"
                     /locus_tag="WGO49_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ0306009.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1547..2242
                     /gene="rplA"
                     /locus_tag="WGO49_000884"
     CDS             1547..2242
                     /gene="rplA"
                     /locus_tag="WGO49_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ0306010.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKITRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2472..2972
                     /gene="rplJ"
                     /locus_tag="WGO49_000885"
     CDS             2472..2972
                     /gene="rplJ"
                     /locus_tag="WGO49_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ0306011.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3030..3401
                     /gene="rplL"
                     /locus_tag="WGO49_000886"
     CDS             3030..3401
                     /gene="rplL"
                     /locus_tag="WGO49_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ0306012.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3592..7770
                     /gene="rpoB"
                     /locus_tag="WGO49_000887"
     CDS             3592..7770
                     /gene="rpoB"
                     /locus_tag="WGO49_000887"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ0306013.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNRLYNGSGKKEDLDSLTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSEDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7927..12102
                     /gene="rpoC"
                     /locus_tag="WGO49_000888"
     CDS             7927..12102
                     /gene="rpoC"
                     /locus_tag="WGO49_000888"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ0306014.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKVALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRERILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            12360..12731
                     /gene="rpsL"
                     /locus_tag="WGO49_000889"
     CDS             12360..12731
                     /gene="rpsL"
                     /locus_tag="WGO49_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ0306015.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            12849..13319
                     /gene="rpsG"
                     /locus_tag="WGO49_000890"
     CDS             12849..13319
                     /gene="rpsG"
                     /locus_tag="WGO49_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ0306016.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            13338..15443
                     /gene="fusA"
                     /locus_tag="WGO49_000891"
     CDS             13338..15443
                     /gene="fusA"
                     /locus_tag="WGO49_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ0306017.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4144 a         3446 c         4011 g         3927 t
ORIGIN      
        1 tttagaggcc aatagctcaa ttggtagagt atcggtctcc aaaaccgagg gttgggggtt
       61 cgagaccctc ttggcctgcc aaataaaaaa ttaaccggcc ttgtgccggt taattttttt
      121 gtatttgtta tttagtaaac tctcttgcca tttacatgga ttgagaatag acagatgcta
      181 tgatggataa ataatatgac agaacatacg cctgaaaaaa agaacgttaa agtggatcaa
      241 ctggttgttc aagataaaga atctgcatct aattccggta aggaagggtt ttttgcatat
      301 ttctcaaatt cttggtccga attcaaaaag gtggtttggc ctaagcgtga agatgctgtc
      361 agaatgactg tatttgttat agtgtttgtt gctgtgcttt ctatatttat ctatgcggca
      421 gatacagcaa tttcgtggtt attttttgat gtattgctga gaagggaagg ttgagatgtc
      481 gaaaaaatgg tatgttgtac aggcgtattc ggggtttgag aagaatgtcc aacgaatatt
      541 ggaagagcgt attgcccgtg aggagatggg agattatttc ggacaaattc tggtgcctgt
      601 agagaaagtt gttgatatcc gcaatggtcg taagactatt agtgaaagaa agtcatatcc
      661 tggttatgtg ctagttgaga tggaaatgac agatgactct tggcatcttg taaaaagcac
      721 cccccgtgtt tctggtttta ttggagggag ggctaataga cctacgccga ttagtcagag
      781 agaggctgaa attattttac agcaggttca gaccggcata gagaagccga aaccaaaagt
      841 tgaatttgag gtcggtcaac aggttcgtgt aaatgaaggg ccgtttgcgg attttaacgg
      901 ggtggttgag gaggtcaatt atgaacggaa taagttacgc gtgtctgttc agatatttgg
      961 tagagaaaca cccgttgagc tggagttcag ccaggttgaa aagattaact gatttttata
     1021 cttgaaaaaa aagcaataag aggatagaat caaaaattaa cttggggagc ggaaatggtt
     1081 ccgcgtctta cccgttttta ggagtccgtt aagtggcaaa gaaaattatc ggctatatta
     1141 aactgcaaat tcctgcaggt aaagccaatc catctcctcc ggttggtcct gctttgggtc
     1201 agcgcggttt gaatattatg gaattttgta aggcatttaa tgctgcaacc caaggtatgg
     1261 agcctggctt accgattccg gttgtgatta ctgcatttgc agataaatca ttcacatttg
     1321 tgatgaaaac cccgccagct tctatcttgt tgaaaaaggc tgccggtttg caaaaaggta
     1381 gttctaatcc tctgaccaac aaagtgggta aattgacccg tgcccagttg gaagaaattg
     1441 ctaaaactaa agagcctgat ttgactgctg ctgacttgga tgcggctgtc cgtactatag
     1501 caggttctgc tcgctcaatg ggcttggatg tggagggtgt tgtataatgg ctaaagtatc
     1561 taaacgcttg aaagctcttc gctcttctgt ggaagccaat aaattatatg caattgatga
     1621 agcaattgct ttggtaaaaa aagcagcgac tgctaaattt gacgagtctg ttgacgtatc
     1681 tttcaacttg ggcgttgatc cgcgtaaatc tgaccaagtt atccgtggtt cggtcgttct
     1741 gcctaaaggc accggtaaga taacccgtgt ggctgtattt actcaaggtg caaatgcaga
     1801 agctgctaaa gaagctggtg cagatatcgt cggtttcgaa gatttggctg ctgaaatcaa
     1861 agcaggcaat ctgaactttg acgttgttat tgcttctccc gatgcaatgc gtattgttgg
     1921 tcaattgggt actattttgg gtcctcgagg cttgatgcca aaccctaaag taggtactgt
     1981 tactcctaac gttgctgaag cagtaaaaaa tgcaaaagca ggtcaagtgc agtaccgtac
     2041 cgacaaggca ggtattgttc atgcaacgat tggtcgtgct tcattcgctg aagctgattt
     2101 gaaagagaac tttgatgcgt tgctggatgc tatcgttaaa gccaagcctg ctgccgctaa
     2161 aggtcagtat ctgaaaaaag ttgctgtgtc tagcaccatg ggtttgggta ttcgcgttga
     2221 tacatcaagc gtaaataact aatcttaagg aattttcaag cagtttggtt ttctgggctg
     2281 cttgaatttg ggctacttaa aattaagtag atgtccaaga ccgtagggat cgtaagattt
     2341 aatcgtaact gccctacgca gacggtagtc ctgaaacaca ttgcaagatt gcttgtaaga
     2401 tgtcttttta ggttaccgcg ctggtgggat atcgttttgg tatcctgttt ataaacagtg
     2461 ggaggtagac cttgagtctc aatattgaaa ccaagaaagt ggcggtcgag gaaattagcg
     2521 cggcaattgc taatgctcaa accctcgtag tcgctgaata tcgcggtatc agtgtttcca
     2581 gtatgactga gcttcgtgcg aatgcacgta aagaaggcgt ttatttgcgc gttctgaaaa
     2641 atactttggc tcgtcgtgca gtgcaaggta cttcatttgc agaattggcc gatcaaatgg
     2701 ttggtccgtt ggtttacgct gcttctgaag atgctgttgc tgctgctaaa gtgttgcacc
     2761 aattcgcgaa aaaagatgac aaaattgtcg ttaaagccgg ttcttacaat ggcgaagtaa
     2821 tgaatgctgc tcaggttgct gagttggctt ctattccgag ccgcgaagag ctgttgtcca
     2881 aactgttgtt cgttatgcaa gctcctgtat cgggctttgc gcgcggtttg gctgctttgg
     2941 cagagaaaaa agccggcgaa gaagccgctt aatcgatttt gtttctgtta atcaattatt
     3001 ttttaataca atatttggag taaaatagca tggctattac taaagaagac attttggaag
     3061 cagttggttc tttgaccgta atggaattga acgacttggt taaagctttt gaagaaaaat
     3121 tcggtgtttc tgctgctgct gttgcagttg caggtcctgc tggtgccggt gctgccgatg
     3181 ctgaagaaaa aaccgaattt gatgtcgttt tggcttctgc cggcgatcaa aaagtcggcg
     3241 tgattaaagt tgtccgtgca attaccggtt tgggtctgaa agaagctaaa gacatcgttg
     3301 acggtgcacc taaaaccatt aaagagggtg tttctaaagc tgaagccgaa gacatccaaa
     3361 aacaactgga agaagccggc gctaaagtcg aaatcaaata atttgatgct tcttatgaag
     3421 gctggcagtt ttctgccagc cttattttgc ttcttaaaat aaacatcaag tattgtttac
     3481 atttatttgc atagttttta tcaaatcatt gcaaataaat gtaaacatca gattgatgcg
     3541 tgccgttgct ttcaaacggc ctattattga aaattacttt tcggagtgta tatgagctat
     3601 tcgtttaccg agaaaaaacg tatccgtaag agttttgcaa agcgggaaaa tgttttggaa
     3661 gttcctttct tgctagcaac ccaaattgat tcttatgcga agtttttgca gctggaaaat
     3721 gcttttgaca aacgtaccga tgacggtctg caggcagcat ttaattctat tttcccgatt
     3781 gtgagccata acggttatgc gcgattggag tttgtgcatt acacattggg cgagcctttg
     3841 ttcgatattc ccgaatgtca gttgcgcgga atcacttatg cagccccctt gcgcgcgcgt
     3901 atccgtttgg tgattttgga taaggaagca tctaaaccga cggtaaaaga agttcgtgaa
     3961 aacgaagtgt atatgggcga aattccgttg atgaccccga gcggttcttt tgtgattaac
     4021 ggcacagagc gtgtgattgt ctcccagttg caccgttcgc ccggcgtatt cttcgagcat
     4081 gacaaaggta agacgcactc ttccggcaaa ttgttattct ccgcccgcat cattccctac
     4141 cgtggttcat ggttggattt tgaatttgat ccgaaagatt tgctgtattt ccgtatcgac
     4201 cgccgccgta aaatgccggt aacgattttg ttgaaggctt tgggctacaa caatgagcaa
     4261 atcttggata ttttctacga caaagaaacg ttctatttgt cttcaaacgg tgttcaaacc
     4321 gatttggtcg caggccgtct gaaaggcgaa actgccaagg tcgatatctt ggataaagaa
     4381 ggcaatgtat tggttgccaa aggtaagcgt attactgcga aaaatatccg tgatattacc
     4441 aatgcaggcc tgacccgttt ggatgtagaa ccggaaagcc tgctgggcaa agcattggct
     4501 gccgatctga ttgattcgga aaccggcgag gtattggctt ctgccaatga tgaaattaca
     4561 gaagagttgt tggccaaatt tgatatcaac ggcgtaaaag aaattacgac cctttacatc
     4621 aatgagttgg atcagggtgc ttatatctcc aataccttgc gtacggatga gactgccggc
     4681 cggcaggcgg ctcgtgttgc gatttaccgt atgatgcgtc cgggcgaacc gcccactgaa
     4741 gaggcggtcg agcaattgtt taaccgcttg ttcttcagtg aagacagcta cgatttgtcc
     4801 cgcgtaggcc gtatgaaatt taatacgcgc acatacgaac aaaaactgtc cgaagcccaa
     4861 caaaactctt ggtacggccg cctgctgaac gaaacgtttg ccggtgctgc cgacaaaggc
     4921 ggttatgtcc tgagcgtcga agatattgtc gcctcgattg cgactttggt cgagttgcgt
     4981 aacggccatg gcgaagtgga cgatatcgat cacttgggca accgccgagt acgttcggta
     5041 ggcgagctga ctgaaaacca attccgcagc ggtttggccc gtgtggaacg tgctgtaaaa
     5101 gaacgtttga atcaggcgga atcagaaaac ttgatgccgc acgatttgat taatgcaaaa
     5161 cctgtttctg ccgctattaa agaattcttc ggctccagcc aattgagtca gtttatggat
     5221 cagaccaacc ccttgtctga agtaacccat aaacgccgcg tatctgcatt gggtccgggc
     5281 ggtttgaccc gcgaacgtgc cggcttcgag gtacgggacg tgcatccgac ccactacggc
     5341 cgcgtatgtc cgattgaaac gcctgaaggt ccgaacatcg gtttgatcaa ctcattgtcc
     5401 gtttatgcgc gcaccaatga ttacggtttc ttggaaacgc cttaccgccg cgttatcgac
     5461 ggcaaagtaa ccgaggaaat cgattacttg tctgccatcg aagaaggccg ctatgtgatt
     5521 gcacaggcga atgccgattt ggattcagat ggcaatctga ttggcgattt ggttacctgt
     5581 cgtgaaaaag gcgaaaccat tatggcaacg cccgaccgcg tccaatatat ggacgtggca
     5641 accggccagg tggtatccgt tgcggcatcc ctgattccgt tcttggaaca tgatgacgcg
     5701 aaccgcgcat tgatgggtgc caacatgcaa cgtcaggcag tgccttgctt gcgtcctgaa
     5761 aaaccgatgg tcggtaccgg tatcgagcgt tccgttgccg ttgactctgc tactgcaatc
     5821 gttgcccgcc gaggcggcgt ggtcgagtat gtcgatgcca accgtgttgt gattcgtgtc
     5881 catgacgacg aagcgactgc cggcgaagtg ggtgtcgata tttacaactt ggttaaattt
     5941 acccgttcca accagtctac caatatcaat cagcgtcctg ccgtcaaagc cggcgatgtt
     6001 ttgcaacgcg gcgatttggt ggccgatggc gcgtccaccg atttgggcga attggctttg
     6061 ggtcaaaata tgaccatcgc cttcatgccg tggaacggtt acaactacga agactcgatt
     6121 ctgatttccg aaaaagtggc tgcggatgat cgttacactt cgattcacat tgaggaattg
     6181 aatgtcgttg cccgcgatac caagctgggt gcggaagaca ttacccgcga tattccgaac
     6241 ttgtccgagc gtatgcaaaa ccgcttggac gaatccggta tcgtttacat cggtgcagaa
     6301 gtagaagccg gcgatgtgtt ggtaggcaaa gtaacgccta aaggcgaaac ccaactgacg
     6361 ccggaagaaa aactgttgcg cgccatcttc ggcgaaaaag cgtctgacgt aaaagatact
     6421 tcactgcgta tgcctaccgg tatgagcggt acggttatcg acgttcaagt cttcactcgt
     6481 gaaggtattc aacgcgacaa acgcgcccaa tccattatcg attccgagtt gaaacgctac
     6541 cgtttggatt tgaacgacca attgcgtatt ttcgacaacg acgcattcga ccgtatcgaa
     6601 cgtatgattg tgggtcaaaa agccaatggt ggtccgatga agctggctaa aggcagcgaa
     6661 attactaccg aatatttggc gggtctgccg agcaggcacg attggttcga tatccgtctg
     6721 accgatgaag atttggccaa gcagttggaa ctgattaaag tgagcctgca acaaaaacgc
     6781 gaagaagcgg acgagttata cgaaatcaag aagaaaaaac tgacccaagg cgacgaattg
     6841 caacccggcg tacaaaaaat ggtgaaagtt tttatcgcca tcaaacgccg tctgcaagcc
     6901 ggcgacaaaa tggcgggccg ccacggtaac aaaggcgtgg tatcgcgcat tctgccagtg
     6961 gaagacatgc cttacatggc ggacggccgt ccggtagaca tcgtactgaa cccattgggc
     7021 gtaccttccc gtatgaacat cggtcagatt ttggaagttc acttgggttg ggcagcaaaa
     7081 ggtatcggcg agcgtatcga ccgtatgctg aaagagcaac gcaaagcagg cgagttgcgc
     7141 gagttcttga acagactcta caacggcagc ggtaagaaag aagatttgga cagcctgaca
     7201 gatgaagaaa tcatcgaatt ggcttccaac ctgcgtaaag gtgcatcttt cgcttctcct
     7261 gtattcgacg gtgcgaaaga gtctgaaatc cgcgaaatgt tgaacttggc ttacccaagc
     7321 gaagatcctg aggttgagaa actgggcttc aacgacagta aaactcaaat cacgctgtat
     7381 gacggccgtt caggcgaagc atttgatcgc aaggttacag tcggtgtgat gcactatctg
     7441 aaactgcacc acttggttga cgaaaaaatg cacgcccgtt ctaccggtcc atacagtctg
     7501 gttacccaac agcctttggg cggtaaagct cagttcggtg gccaacgttt cggtgagatg
     7561 gaggtttggg cattggaagc atacggtgcg gcatacacgc tgcaagagat gctgactgtg
     7621 aagtctgatg acgtgaacgg ccgtaccaaa atgtacgaga acatcgtcaa aggcgaacac
     7681 aaaatcgatg ccggtatgcc cgagtccttc aacgtattgg ttaaagagat tcgttcactg
     7741 ggcttggata tcgatttgga acgctactaa gcagaagttt tcagacggcc tttcaaggtc
     7801 gtctgaaaaa gcggtttcag aataagaatg aagcaatcgg catttaggcc gtctgaaatc
     7861 gaaagtaccg tttcccaata tcgaaaatcc gccacgcggt aaaaatactt ccttcaagga
     7921 gcaaaaatga atttgttgaa cttatttaat ccgttgcaaa ctgccggcat ggaagaagag
     7981 tttgatgcca tcaaaatcgg cattgcttct cccgaaacca tccgttcatg gtcttacggc
     8041 gaagttaaaa aacctgaaac catcaactac cgtacgttca aacctgagcg tgacggtctg
     8101 ttctgcgcca aaatctttgg tccggtcaaa gactacgaat gcttgtgcgg aaaatacaaa
     8161 cgcttgaaat ttaaaggcgt aacctgtgaa aaatgcggcg tagaagtgac cctgtccaaa
     8221 gtgcgccgcg aacgcatggg ccacattgaa ttggcagcac ccgttgccca tatttggttc
     8281 ttgaaatccc tgccttcccg cttgggtatg gtactggaca tgactttgcg cgacatcgaa
     8341 cgcgtattgt actttgaagc atttgtggta accgaccccg gtatgactcc gctgcaacgc
     8401 cgccaattgc tgactgaaga cgattactac aacaaactgg acgaatacgg cgatgacttc
     8461 gatgccaaaa tgggtgcgga aggtatccgc gaattgctgc gtaccttgaa tgtggcgggc
     8521 gaaatcgaaa tcctgcgcca agagttggaa tcgaccgggt ccgacaccaa aatcaaaaaa
     8581 atcgccaaac gtttgaaagt attggaagcc ttccaccgtt ccggtatgaa gctggaatgg
     8641 atgattatgg atgtgctgcc ggtattgccg cctgatttgc gtccgttggt tccattggat
     8701 ggcggtcgtt ttgccacttc cgatttgaac gatttgtacc gtcgcgtcat caaccgtaac
     8761 aaccgtctga aacgtctgtt ggaactgcat gcgcccgaca ttatcgtccg caacgaaaaa
     8821 cgtatgttgc aagaagcggt cgactctctg ttggataacg gccgtcgcgg taaagccatg
     8881 accggcgcca acaaacgccc gctgaaatca ttggcagaca tgattaaagg taaaggcggt
     8941 cgcttccgtc aaaacctgtt gggcaaacgt gtggactact ccggccgttc cgtgattacc
     9001 gtaggcccat acctgcgtct gcaccaatgc ggtctgccga aaaaaatggc tttggaactg
     9061 ttcaaaccgt tcattttcca caaattggaa aaacaaggtt tggcctctac cgttaaagca
     9121 gcgaaaaaat tggtagagca agaagtaccg gaagtatggg acatcttgga agaagtcatc
     9181 cgcgaacatc cgattatgct gaaccgtgcg ccgaccctgc accgtttggg tattcaagcg
     9241 ttcgaaccta tcttgattga aggtaaagcg attcagttgc acccattggt gtgtgctgcg
     9301 ttcaacgccg actttgacgg cgaccaaatg gcggtacacg ttccattgag cttggaagca
     9361 caaatggaag cacgcacgct gatgctggct tcaaacaacg tattgtctcc tgccaacggc
     9421 gaaccgatta tcgtaccttc ccaagacatc gtattgggcc tgtactacat gacccgcgac
     9481 cgtatcaatg ccaaaggcga gggcagcctg tttgccgatg tgaaagaagt gcatcgcgca
     9541 taccatacca aacaggttga actgggtacg aaaattaccg tacgtctgcg cgaatgggtg
     9601 aaaaacgaag caggtgagtt tgagcctgtc gttaaccgtt acgaaacaac cgtcggccgt
     9661 gcattgttga gcgaaatcct gccgaaaggc ctgccgtttg aatatgtcaa caaagcgttg
     9721 aagaaaaaag aaatttctaa actgattaac gcatcgttcc gcctgtgcgg cttgcgcgat
     9781 acggttatct ttgctgacca cctgatgtac accggtttcg gatttgcggc aaaaggcggt
     9841 atttccattg ccgttgacga tatggaaatt ccaaaagaaa aagtggcctt gctggctgaa
     9901 gccaatgccg aggttaaaga aatcgaagac caataccgtc aaggtttggt taccaacggc
     9961 gaacgctaca acaaggtggt cgatatttgg ggtcgtgccg gcgataagat tgctaaagcg
    10021 atgatggaca acttgtccaa acaaaaagtt atcgaccgtg acggcaacga agtcgatcaa
    10081 gagtcattca actccattta tatgatggcg gactccggtg cccgtggttc tgcagctcag
    10141 attaaacagt tgtccggtat gcgtggcttg atggcaaaac ctgacggctc gattattgaa
    10201 acgccgatta cctcaaactt ccgtgaaggt ctgaccgtat tgcaatactt tattgcgacc
    10261 cacggtgcgc gtaagggttt ggcggatacc gcattgaaaa ccgcgaactc cggttacctg
    10321 acccgtcgtc tggtagacgt aactcaagat ttggtcgttg ttgaagacga ttgcggtact
    10381 tcagacggct ttgtcatgaa ggcagtggta caaggcggtg atgtgattga agcattgcgc
    10441 gagcgtattt tgggccgtgt tactgcgtct gacgttgtcg atccgtcaag cggcgagacc
    10501 ttggttgaag caggcacgtt gttgactgaa aaactggtgg atatgattga ccaatccggt
    10561 gtcgatgaag tcaaagtccg tactccgatt acttgtaaaa cccgccacgg tttgtgcgcg
    10621 cactgttacg gccgcgactt ggcgcgcggc aaactggtca atgccggaga ggcagtcggc
    10681 gtgattgccg cccaatcaat cggcgaaccg ggtacccagc tgactatgcg tacgttccac
    10741 atcggtggtg cggcatcccg tgcggcagca gccagccaag tggaagccaa atccaacggt
    10801 acggcacgat tcagcagcca aatgcgttat gttgccaaca ataaaggcga gttggttgtc
    10861 atcggccgtt cttgtgaagt cgtgattcac gatgacatcg gccgtgaacg cgaacgccac
    10921 aaagtacctt acggtgccat cctgctggta caagacggta tggccattaa agccggtcaa
    10981 accttggcaa cctgggatcc gcatacccgt ccgatgatta ccgaacacgc aggtatggtg
    11041 aaattcgaaa acgtggaaga gggcgttacc gttgccaaac aaaccgatga tgtaaccggt
    11101 ttgtccactt tggtagtgat cgacggtaaa cgtcgttcct ctagtgcttc caaactgctg
    11161 cgtccgactg tgaaactctt ggacgaaaac ggcgtggaaa tctgtattcc cggtacttct
    11221 actccggtat ccatggcatt ccccgttggt gcggtgatta ccgtacgcga aggtcaggaa
    11281 atcggtaaag gcgacgtatt ggcgcgtatc ccgcaagcct cttccaaaac ccgcgacatt
    11341 accggtggtc tgccgcgcgt tgccgaattg tttgaagcac gcgtgccgaa agatgccggt
    11401 atgttggcgg aaattaccgg taccgtttcc ttcggcaaag agaccaaagg caagcaacgt
    11461 ctgattatta ccgacgtgga cggcgtagca tacgagactt tgatttccaa agagaaacag
    11521 attttggtac acgacggtca agtggtaaac cgcggcgaaa ccattgtgga cggcgcggtc
    11581 gatccgcacg atattctgcg tctgcaaggt atcgaagcac tggcacgcta cattgtccaa
    11641 gaggtgcaag aggtttaccg tctgcaaggt gtgaagattt ccgacaaaca catcgaagtc
    11701 atcatccgtc aaatgttgcg ccgtgtgaac attgcggatg ccggcgaaac cggcttcatt
    11761 accggcgagc aggtcgaacg cggcgatgtg atggctgcca atgaaaaagc tttggaagaa
    11821 ggcaaagaac cggcacgtta cgaaaacgta ttgctgggta ttaccaaagc ctccctgtct
    11881 accgacagct tcatttctgc cgcatcgttc caagaaacga cccgcgttct gaccgaagcc
    11941 gcgattatgg gcaaacaaga cgagctgcgc ggtctgaaag agaacgtgat tgtcggtcgt
    12001 ttgattcctg ctggtactgg tttgacttac caccgcagcc gtcatcaaca atggcaagag
    12061 gtggagcagg agactgctga aacccaagta acggatgaat aatctttggt gcgtttattc
    12121 aatacaaaac cgcaagcttt aggcttgtgg tttttctttc agacggcatt taaattatta
    12181 gtgatttcct ttccggtaat agttgtttat gagcataagg gacctaaatg tttatctgaa
    12241 aaaagaaaaa tgatgaatct tcctgatctc acgcttgact gtctgcttgc aataaaaata
    12301 taattccact cttgccgaca tggtgtcggc aagtatttaa ctcaacagga cgagaaaata
    12361 tgccaactat caaccaattg gtacgcaaag gccgtcaaaa gcccgtgtac gtaaacaaag
    12421 tgcccgcact ggaagcctgc ccgcaaaaac gcggcgtgtg cacccgtgta tacacgacta
    12481 cccctaaaaa acctaactct gcattgcgta aagtatgtaa agtccgcctg accaacggtt
    12541 ttgaagtcat ttcatacatc ggcggcgaag gccacaacct gcaagagcac agtgtcgtat
    12601 tgattcgcgg cggtcgtgta aaagacttgc caggtgtgcg ttaccacact gtacgcggtt
    12661 ctttggatac tgcaggcgtt aaagaccgta aacaagcccg ttccaaatac ggtgctaagc
    12721 gtcctaaata attactggga cttaaatagg cacgtcggcc gcctaagctg aacaacggcc
    12781 gagtaagtga atactcaatt gggtattcat gggaatagac ccgactgaat agattaaagg
    12841 aaattaaaat gccaagacgt agagaagtcc ccaagcgcga cgtactgcca gatcctaaat
    12901 tcggcagcgt cgagttgacc aaattcatga acgtattgat gattgacggt aaaaaatccg
    12961 ttgccgagcg tatcgtttac ggtgcgttgg aacagattga gaaaaaaacc ggcaaagtag
    13021 caatcgaagt atttaacgaa gccattgcaa acgccaagcc tatcgtggaa gtgaaaagcc
    13081 gccgtgtagg tggtgcaaac taccaagttc ctgttgaagt tcgtccttca cgccgtttgg
    13141 ctttggcaat gcgctgggtt cgcgatgcgg cccgcaaacg tggtgagaaa tccatggacc
    13201 tgcgtttggc aggcgaattg attgatgcgt ccgaaggtcg tggcggtgcg ttgaaaaaac
    13261 gtgaagaagt acaccgtatg gctgaagcca acaaagcatt ctctcacttc cgtttctaat
    13321 tttgaaaggc taataaaatg gctcgtaaga ccccgatcag cctgtaccgt aacatcggta
    13381 tttccgccca tatcgatgcg ggtaaaacca cgacgacaga acgtattttg ttctatactg
    13441 gtttgaccca caagctgggc gaagtgcatg acggtgcggc tactaccgac tacatggaac
    13501 aagagcaaga gcgcggtatt accattacct ccgctgccgt tacttcctac tggtccggta
    13561 tggcgaaaca attccccgag caccgcttca acatcatcga caccccggga cacgttgact
    13621 ttaccgtaga ggtagagcgt tctatgcgtg tattggacgg cgcggtaatg gtttactgcg
    13681 cggtgggcgg tgttcaaccc caatctgaaa ccgtatggcg gcaagccaac aaataccaag
    13741 tgccgcgctt ggcgtttgtc aataaaatgg accgtcaggg tgccaacttc ttccgcgttg
    13801 tcgagcaaat gaaaacccgt ttgcgcgcaa accctgtacc tatcgtcatt ccggttggtg
    13861 cggaagacaa cttcagcggt gtggttgatt tgttgaaaat gaaatccatc atttggaatg
    13921 aagccgataa aggtacaacc tttacctatg gcgatattcc tgccgaattg gtcgaaactg
    13981 ccgaagaatg gcgtcaaaat atgattgaag ccgcagccga agccagcgaa gaactgatgg
    14041 acaaatactt aggcggcgac gagctgaccg aagaagaaat cgtaggcgcg ttgcgtcaac
    14101 gtactttggc aggcgaaatt cagcctatgc tgtgtggttc tgcatttaaa aacaaaggtg
    14161 ttcaacgtat gttggacgca gttgtagaat tgctgccagc tcctaccgat attcctccgg
    14221 ttcaaggtgt caacccgaat accgaggaag ccgacagccg tcaagccagc gatgaagaga
    14281 aattctctgc attggcgttc aaaatgttga acgacaaata cgtcggtcag ctgaccttta
    14341 tccgcgttta ctcaggcgta gtaaaatccg gcgataccgt attgaactcc gtaaaaggca
    14401 ctcgcgaacg tatcggtcgt ttggtacaaa tgactgccgc agaccgtact gaaatcgaag
    14461 aagtacgcgc cggcgacatc gcagccgcta ttggtctgaa agacgttact accggtgaaa
    14521 ccttgtgtgc ggaaagcgcg ccgattatct tggaacgtat ggaattcccc gagccggtaa
    14581 tccatattgc cgttgagccg aaaaccaaag ccgaccaaga gaaaatgggt atcgccctga
    14641 accgcttggc taaagaagac ccttctttcc gtgtccgtac agacgaagaa tccggtcaaa
    14701 ccattatttc cggtatgggt gagctgcact tggaaattat tgttgaccgt atgaaacgcg
    14761 aattcggtgt ggaagcaaat atcggtgcac ctcaagtggc ttaccgtgaa actatccgca
    14821 aagccgttaa agctgaatac aaacacgcga aacaatccgg tggtaaaggt caatatggtc
    14881 acgttgtgat tgaaatggaa cctatggaac cggggggcga aggttacgag tttatcgatg
    14941 aaattaaagg tggcgtgatt cctcgcgaat tcattccgtc tgtcgataaa ggtatccgcg
    15001 atacattgcc taacggtatc gttgccggct atcctgtagt tgatgtacgt atccgtctgg
    15061 tattcggttc ttaccatgat gtcgactctt cccaattggc atttgaattg gccgcttctc
    15121 aagcgtttaa agaaggtatg cgtcaagcat ctcctgccct gcttgagccg attatggcag
    15181 ttgaagtgga aactccggaa gaatacatgg gcgacgtaat gggcgacttg aaccgccgtc
    15241 gcggtgttgt attgggtatg gatgatgacg gtatcggcgg taaaaaagtc cgtgccgaag
    15301 tacctctggc agaaatgttc ggttattcga ccgacctgcg ttctgcaacc caaggccgcg
    15361 ctacttactc tatggagttc aagaaatatt ctgaagctcc tgcccacata gctgctgctg
    15421 taactgaagc ccgtaaaggc taatcaggca aataggtcgt ctgaaaggct gaaatgattt
    15481 ttcagacggc attgttcttt aatcgatctt taatgtaaag gaattagc
//
LOCUS       DAWFAD010000039        15224 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWFAD010000039 DAWFAD010000000
VERSION     DAWFAD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15224
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.039"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..139)
                     /locus_tag="WGO49_000892"
     CDS             complement(<1..139)
                     /locus_tag="WGO49_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0306018.1"
                     /translation="MSVQTVTYNKFFMNTNQPAVYDPLTRALHWLTVAGFIGILTTIV
                     LW"
     gene            380..1534
                     /gene="obgE"
                     /locus_tag="WGO49_000893"
     CDS             380..1534
                     /gene="obgE"
                     /locus_tag="WGO49_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ0306019.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ENVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDSPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEQAAAKAEIAEQQPKTDTGVFKPE"
     gene            1617..2666
                     /locus_tag="WGO49_000894"
     CDS             1617..2666
                     /locus_tag="WGO49_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0306020.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3354..4775
                     /locus_tag="WGO49_000895"
     CDS             3354..4775
                     /locus_tag="WGO49_000895"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ0306021.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGRGRPGWHIECSAMSENLFG
                     NTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPPADPMPSEAGDDYTRRFYVAMNDDFDTVKAVAVLFELAGEVNKTNNAQL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARSDKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4858..5637
                     /gene="xth"
                     /locus_tag="WGO49_000896"
     CDS             4858..5637
                     /gene="xth"
                     /locus_tag="WGO49_000896"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0306022.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5624..5968
                     /locus_tag="WGO49_000897"
     CDS             5624..5968
                     /locus_tag="WGO49_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306023.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            5979..6509
                     /locus_tag="WGO49_000898"
     CDS             5979..6509
                     /locus_tag="WGO49_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0306024.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVPEHGGVPHGWAQKQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6602..7717)
                     /gene="asd"
                     /locus_tag="WGO49_000899"
     CDS             complement(6602..7717)
                     /gene="asd"
                     /locus_tag="WGO49_000899"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ0306025.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGAETNKILGRSDNPTVIDGLCVRIGSMRCHS
                     QALTLKLKKDLPISEIEAILAGANDWVKVIPNEKEASIRELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            7893..8474
                     /locus_tag="WGO49_000900"
     CDS             7893..8474
                     /locus_tag="WGO49_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0306026.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8536..9390)
                     /gene="folD"
                     /locus_tag="WGO49_000901"
     CDS             complement(8536..9390)
                     /gene="folD"
                     /locus_tag="WGO49_000901"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ0306027.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQNNLHTPCLAVVLVGGD
                     PASAVYVRNKKTSCQKCGIKSLSYELPESTSQEELLALVDRLNVDSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9613..9690
                     /locus_tag="WGO49_000902"
     tRNA            9613..9690
                     /locus_tag="WGO49_000902"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9648..9650,aa:Pro,seq:tgg)
     gene            9715..9791
                     /locus_tag="WGO49_000903"
     tRNA            9715..9791
                     /locus_tag="WGO49_000903"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9749..9751,aa:Arg,seq:tct)
     gene            9819..9894
                     /locus_tag="WGO49_000904"
     tRNA            9819..9894
                     /locus_tag="WGO49_000904"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9852..9854,aa:His,seq:gtg)
     gene            10030..10536
                     /locus_tag="WGO49_000905"
     CDS             10030..10536
                     /locus_tag="WGO49_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ0306028.1"
                     /translation="MVDAWSVPDFESKICPPEALSERLARLPRPLVFTNGCFDILHRG
                     HVTYLAQARSAGAALVLALNTDASVRRLGKDGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10533..12311
                     /locus_tag="WGO49_000906"
     CDS             10533..12311
                     /locus_tag="WGO49_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ0306029.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKVDGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            12424..13248
                     /locus_tag="WGO49_000907"
     CDS             12424..13248
                     /locus_tag="WGO49_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ0306030.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(14011..14799)
                     /locus_tag="WGO49_000908"
     CDS             complement(14011..14799)
                     /locus_tag="WGO49_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ0306031.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSIQTAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
     gene            complement(15013..15207)
                     /gene="thiS"
                     /locus_tag="WGO49_000909"
     CDS             complement(15013..15207)
                     /gene="thiS"
                     /locus_tag="WGO49_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ0306032.1"
                     /translation="MNIILNGGPAELHGTSVADLIAQTAPQQPFAVAVNTVFIPKGAY
                     AETVLNENDKIDIVRPVVGG"
BASE COUNT         3626 a         3850 c         4244 g         3504 t
ORIGIN      
        1 tccacaggac aatggtggtc agaatgccga tgaaaccggc aacggtcagc cagtgcagcg
       61 cgcgtgtcaa cgggtcgtaa acggcaggtt ggtttgtgtt cataaaaaac ttattatatg
      121 taacggtttg aacggacatt ccgtcgggtc ggaatgtcaa aaaggcgcga ttgtaccaaa
      181 gaagttggac ataattttgt ttgcaggctg aagatttgct taaaaattca ttaagatggg
      241 cggaacaaat agtttgggta cggcgtgttg aaataccgct tatcccttat aatagcgtcc
      301 gaaattctgt tcagacggca tcaaaacaca cggtaatccg tctgaacccc catttgaaac
      361 caacaaacaa gagcattgaa tgaaattcat cgacgaagca aaaatcgaag tcgccgcagg
      421 caaaggcggt aatggcgcaa ccagtttccg ccgcgaaaaa ttcgtaccgc gcggcggtcc
      481 ggacggcggc gacggcggca aaggcggcag cgtttgggca gaagccgacg aaaacaccaa
      541 caccctcgtc gaataccgct tcgttaaacg ctaccaagcc aaaaacggcg aaaaaggcca
      601 cggttccgac cgttacggcg caggtgcgga cgacatcgtc ctcaaaatgc ccgtcggcac
      661 ccttatccgc gacctcgaca ccggcgaaaa cgttgccgac ctcacttatc acggacagcg
      721 cgtctgcctt gccaaaggcg gcaaaggcgg cttgggcaac atccacttca aatcctccgt
      781 caaccgcgcc ccaaaacaat ccacgcccgg cgaagaaggc gaagcccgtt ccctgcaact
      841 cgaactcaaa gtcctcgccg atgtcggctt attgggtatg cccaacgccg gcaaatccac
      901 cctgattacc gccgtatccg ccgcacgccc caaaatcgcc aactacccct tcaccaccct
      961 gcatccaaac ttaggcgtgg tacgcatcga cgaaaaccac agcttcgtga tggccgacat
     1021 ccccggcctg attgaaggcg cggcagaagg cgcgggtttg gggcatcgtt tcctcaaaca
     1081 cttatcgcgc accggattat tgctgcacgt cgtcgatttg gcgcccttcg acgaaaccgt
     1141 caacccggcc gaagaagcac ttgccatcat caacgaattg cgcaaatacg atgaagaact
     1201 ttacggcaaa ccgcgctggc tggtgctgaa taaactcgac atgcttgacg aagaagaagc
     1261 ccaaacgcgc actgccgcct tcctcgaagc cgtcggctgg gactccccca aacccgacga
     1321 ccgtttccaa tttgacatgg aaaccccgcg cctcttccaa atcagcgcgc tgacccacca
     1381 aggcacgcag gaattggtac accaaatcaa ccaatacctg accgagaaaa aacgtatcga
     1441 agccgaaaaa gcagaagcgg aacaggcagc ggcaaaagcc gaaattgccg agcagcagcc
     1501 gaaaacggat acgggcgtgt ttaagccgga gtgaaaaaag aaagggtttc agacgatatt
     1561 tgttctacaa aaggttgttt gaaacaattt taaagcaaga ttgaaatgga ttaaaaatgt
     1621 catctaacca atatatccag tcgcttgagt tgacaaactt taccgttctt ccaaacgata
     1681 cttttgaatt ttctgaaaat ctcaatgtta ttgttgccga aaacggttgt ggcaaaacac
     1741 atttgttaaa aattttatac agcctgctag aagtaacatc taacaccaaa aacagattac
     1801 tgaaaacaga attgcaaaaa agttttgccg ataaattact gaatgttttc cgtcccgatt
     1861 ccttgggcag gttgagcaaa cgtttgcagg ggcgcgggcg tacggaaatc gttttaaaac
     1921 tgcaaaacgg cactacgcat agtagtctga atttttccag taactcatca tcgcaagtca
     1981 atgttcaaag catcggattg aaagaaaatg aatatactcc gacacctatc ttcttaccca
     2041 gccgcgaatt aattacactt tgcccttggt tcacttcttt gtatcaaaac caaagcatcc
     2101 ctttcgaaga aacttggttt gatgcctgta tgcaattaaa tcacccgctt gccaaaggcc
     2161 ccagagaaac aaaaattcga gaattgctcg aacctattga gaacgcgatg ggcggaaaag
     2221 tatctgaaga acaaggaagg ttttatctat cgctgacaaa tacgggcggt aagattgaag
     2281 cacctttggt agcagagggt ttgcggaaat ttgtcatgat tgcccggtta atcgcaacag
     2341 gggcattgct ggataaaggc tatctatttt gggatgaacc ggaagctaat ctgaatccta
     2401 aattgatcaa agtggctgcc cgtattattt ggtctttatc tcaacagggt attcaggttt
     2461 ttattgcaac acactcttta ttcttattga gagaattgga acttttgaag cttgaaaata
     2521 acacggattt acctgtacgt tttttctcgt tgattgcttc cgatgatgga acaaaagtgg
     2581 agcaagggaa cagtattaat gatttgaaca cattgatatt gttagatgaa aacattatgc
     2641 aggcagacag gtatctgaat atttgaggtt aagctatgac tacaatcaca gaaaagagat
     2701 taacctttgc ttttcctgaa gattattacg ttaccaagta tgacgaatgg gaacattata
     2761 aaatttttca gaacagttgt aatttacgta ataaaattga tacaaatgaa aagggaaaga
     2821 acgggataaa tcaaagtgta gatgacgata atggcagctc aggtgtagat ataatagccc
     2881 ttcatgaatc gacactttgg ctaatcgaaa ttaaagatta ttaccgactt gggcttgagc
     2941 caaatgccca atcaatagat gaaaaacttt ctgacctacc atatttaatc gcaagaaaaa
     3001 ttcgagattc attggcagga ttggtttctg ctaaatttaa agcagaaaaa caggaagaaa
     3061 aagatttttc tcgcttggct ttagattgta acgaaataaa aatagtcctt catattgaaa
     3121 tgcctagtat aagaagtaaa ctttatccct cgtcatcaga tttagcaaac ttattaaaag
     3181 ataagtttaa attatcagaa tttacaaaaa attttgcaaa ctgttatgca gaaccaattt
     3241 tcacaaatat cagccatatt aataatccgc aactacgtaa tgttccttgg tccgtatcta
     3301 caggaacaga acagaaatta tcatccgagc aacaaagatt aatccatagt cccatgacca
     3361 ccatctacaa cacccttacc cgccaaaaag aaccctttgc ccccatcgac cctgaaaacg
     3421 tgcgtatgta cgtttgcggc atgactgttt acgactattg ccacttaggc catgcccgtg
     3481 tgatggtggt gtttgacatg attgcccgtt ggttgcgcga gtgcggctat ccgctcactt
     3541 atgtacgcaa catcaccgac atcgacgaca aaatcattgc ccgtgcggct gaaaacggcg
     3601 agaccatcgg cgagctgact gcgcgtttca ttcaggctat gcacgaagat gccgatgctt
     3661 tgggcgtgtt gcgtcccgat atcgagccga aggcgacgga aaacattccg caaatgattg
     3721 cgatgattga aaccctgatt caaaacggca aggcttatcc tgccgccaac ggcgacgttt
     3781 actacgccgt gcgcgagttt gccgcttacg gccaattatc gggcaaatcg ttagacgacc
     3841 tgcgtgcagg tgagcgtgtg gaagtggacg gtttcaaacg cgatccgctt gattttgtgt
     3901 tgtggaaagc ggcgaaagca ggcgaaccgg cgtgggaaag cccttggggc aggggacggc
     3961 cgggctggca cattgaatgc tccgccatga gtgaaaacct gttcggcaat accttcgaca
     4021 tccacggcgg cggcgcggat ttgcagtttc cgcaccacga aaacgaaatt gcccaaagcg
     4081 tcggcgcgac ggggcatacc tgcggtcatc atcacgcgca aacccaccac gggcaaagca
     4141 tcgccagcca cgtcaaatat tggctgcaca acggctttat ccgcgtggac ggcgaaaaaa
     4201 tgtccaaatc actgggcaac ttcttcacca tccgcgaagt gttgaaacaa tacgaccccg
     4261 aagtcgtgcg cttcttcatc ctgcgcgccc actaccgcag cccgttgaac tattccgacg
     4321 cgcatttgga cgacgcaaaa ggcgcgttga cgcgtttgta tacgactttg aaaaatacgc
     4381 cgcccgccga tccgatgccg tctgaagccg gcgatgacta cacgcgccgc ttttatgttg
     4441 cgatgaatga tgattttgat acggtgaaag cggtggcggt gttgttcgaa cttgccggcg
     4501 aagtgaacaa aaccaataac gcgcaactgg caggctgcct gaaagccttg ggcggcatca
     4561 tcggactgct gcaacgcgac ccgaccgagt tcctgcaagg cggcgcggct tcagacggcc
     4621 tctccaacga agaaatcgaa gacttgattg cccggcggaa acaggcgcgc tccgataaaa
     4681 actgggcgga atccgaccgc attcgcgacc ttctgaacga acacaaaatc attttggaag
     4741 acaacgccgg cggcacgact tggcggcgcg gttgaaatcc atcgaaaagg gtaaatgtgc
     4801 cgcattttgc ccttttttgt tcagacggca tttttattgt gagaacttat ttttattatg
     4861 cttaaaatca tttccgccaa cgtcaacggc atccgctccg cctacaaaaa aggtttttac
     4921 gaatatatcg ccgcatcggg cgcggacatt gtctgcgtgc aggaactcaa agcgcaggag
     4981 gctgatttgt ctgccgatat gaaaaatccg cacgggatgc acggccattg gcattgcgcc
     5041 gagaagcgcg gttacagcgg cgtggcggtg tacagcaaac gcaaacccga caatgtgcaa
     5101 atcggtatgg gcattgaaga attcgacagg gaagggcggt ttgtgcgttg cgatttcgac
     5161 aggttgagcg ttatttcgct ttacttgccc agcggcagca gcgcggaaga acgccagcag
     5221 gtgaaatacc gtttccttga tgcgttttac cctatgctcg aagcgatgaa aaacgaagga
     5281 cgcgacattg tcgtttgcgg cgactggaac atcgcccacc aaaacatcga cctgaaaaac
     5341 tggaaaggca atcagaaaaa ttcgggcttc ctaccggaag aacgcgagtg gataggcaag
     5401 gttatccaca agctcggttg gacggatatg tggcgcacgc tttatcccga tgtgccgggc
     5461 tacacgtggt ggagcaaccg cgggcaggcg tatgcgaaag atgtcgggtg gcgcatcgat
     5521 tatcagatgg ttacgcccga acttgccgcc aaagccgtgt ccgcacacgt ttataaagat
     5581 gaaaaatttt ccgaccatgc gccgctggtc atggagtatg actatgctgc cgaataaagt
     5641 tttgggtaaa tatgattgga atgtggacgg aaaaacagga ataggcgcag catgggttgt
     5701 cgctgcgttt atcctgccca tattggtgtg ggcgatattt atgttgtcgc ggatgcaagg
     5761 ctggcttgcg ccgaccaagg caaacccgac atgggcgttg gtgtggctgc tgatctgcat
     5821 gccctgcctg ctgattgcgt ccaaatgttt gggttggaaa ggctggcggc gggtggtgaa
     5881 tatttttgcc tgcctgaccg tctgcgccat attgagcgta cccgcttcgc tgctcatcgc
     5941 ctttaccctc cgggacctgc tgaaataggc ggggcggtat gcagggtttt gaactcgaag
     6001 atttgacgct ttggctgata cgcgatgccg gagaggatca gatgtggata gaccgttggg
     6061 cgatcagtta tcccgtcgtg cagatgtccg aagcgtcggc cggtcaaagc ataggggaat
     6121 ggcaggcagg gcttcggacg gcatttgaac gcatacgtgg caaatacgtc gccgttgtcg
     6181 cacacggtgc gggcgcggcc gcatttttgg cgtggctgta tcaggcagac atcctgacac
     6241 ggaagaaaat tgccaacatc atccttgtac cgcagcgtcc cgatattttt cccgacgatg
     6301 cggaacacac tttccaacgc gtccgctgtc cctgccgtgc cgcattggtt gtacccgaac
     6361 acggcggcgt gccgcacggt tgggcgcaaa aacaggcgga tttgtggaac gcccgcctgt
     6421 tggtttcccc gcattcgggc agtttgaacg gcatgctcgg cggctggcag tggggtatga
     6481 agctgatgca ggaaatgttg ctggcgtgaa cgcttgcctt atcggaattt cggttaactg
     6541 gggttgcaag gaaaatgccg tctgaaacag gcttcagacg gcatttccat tcaaacaggg
     6601 cttacaggct gcccaacacg atacgcaata cgcggcgcag cggctcggcc gcgccccaca
     6661 agagctggtc gccgacggtg aacgcgctga tgtattcgcc gcccataccc agtttgcgga
     6721 tgcgtccgac ggggacggac agcgtgccgg taactttggc gggggtcagc tcgcggatgc
     6781 tggcttcttt ttcgttgggg atgactttca cccagtcgtt cgcgcctgcc aaaatcgctt
     6841 cgatttcgga aatgggcagg tcttttttca acttcagggt aagggcttgg ctgtggcagc
     6901 gcatagagcc gatgcggacg cacaggccgt caatcacggt tggattgtcg ctgcggccga
     6961 ggattttgtt ggtttccgcg ccgcctttcc attcttcttt ggactggccg ttgcccaaat
     7021 ccacgtcaat ccacggaatc aggctgccgg cgagcggtac gccgaagttg gctttcggat
     7081 agtcttcgct gcgcaggaaa tcagatactt tgcggtcgat gtcgagaatc gagccggcag
     7141 gatcggcaag ttcgtccgcc acttgggcgt gaaccgcgcc cataccgctg atgagttcgc
     7201 gcatgttttt cgcgcctgcg ccggaagcgg cttggtaggt catgctggtt gcccattcga
     7261 ccaaatcgtt ttggaacagg ccgcccaaag ccatcagcat cagggaaacg gtacagttgc
     7321 cgccgatgta gtttttcacg ccgtttttga ggccgttgtc gatgacgttg cggttgacgg
     7381 ggtcgaggac gataatcgcg tcgtctttca tacgcagcga ggatgccgcg tcaatccagt
     7441 agccgttcca gccgctgtcg cgcagggctt ggaagacgga tttggtgtag tcgccgcctt
     7501 ggcaggtaac gatgatgtcc atttttgcca gctcggcaac gttgttcgcg tccaataatg
     7561 ttttagccgc ctgaccgaaa tcaggggctg cgccgccgac gttggaagtg gtaaagaaaa
     7621 acgcttcggg aatgtgggcg aagtcgtttt cttctttcat acgctgcatc aaaaccgaac
     7681 cgaccatacc gcgccagccg acgaaaccta ctttcattgt gttgctcctt gaggaaaaat
     7741 cgttaataaa attatgtatg gcgtaaagga agcgttttaa cgccgtattg ggaaaatgta
     7801 aagttcttta tcgggatttc tttaagtatt ggcgttttcg gtgtcgtggc ggtagaatcg
     7861 gggtcagtta agaacaatta tcaggaacgg atatggaaga aaaaaacgac tacaccgacg
     7921 cggcatccga caaccgcaac gggcaggaaa ttgaagtcgg aattgccggc gcaggcgacc
     7981 ggatacttgc cgcgctgctg aaccaattgt ttaccttttt ggttttgttg gtgccgtttg
     8041 ccggactgat tgctttcgcc atcaagaatg aaggcaggat tggcggcagt gaggaaatat
     8101 tcgggctgct tttgggcatg acttcctttt gggtaggact ggcgggcatt ttggtttata
     8161 ccgtcgttca aatctactat atgagtcggg acgggcagtc attgggtaag aaaatcatga
     8221 gaatcagggt gttgaaaacc gacggccgca atcccggttt tgtcggcacg gttttggtac
     8281 gcgaaatcgc atggtcggtt ttggttgcca ttattgccgc cgttatcggt cttgcagtag
     8341 gtgacaacgg agaaaacgcc atcaacctgc tggcattcct tgccaacttt gtcctgctct
     8401 ttatggtcaa acgcgatcgc cgcacgcttt acgacatact ggcggatacg gttgtcgtca
     8461 agctgcccaa ataaacggac acggcaaaat gccgtctgaa agcctttcag acggcatttt
     8521 tatcttcaga accgctcaag catcgtgcag tgaagccgcg tgcagggtgt tttccatcaa
     8581 tgtggcaatc gtcatcggac ccacgccgcc gggaacgggc gtaatcatcg ccgcccgttc
     8641 ttttgccgtt tcaaattcca cgtcgccgca caggctgccg tcgtccagac ggttgatgcc
     8701 cacatcaata acgaccgcgc cgggtttgat ccattcgcct ttgacaaagt tcggaatgcc
     8761 tacgccgacc accaaaatat cggccccggc aacctcgtct gccagatttt cggtcgcact
     8821 gtggcagacc gttaccgttg cgcgcgccag cagcaattcc aaagcctgcg ggcggccgac
     8881 aatattcgac gcgccgacca cgaccgcttt tttccccttc ggatcgatgc cgtaagcctc
     8941 caaaagcgtc atcacgccct tgggcgtaca cgggcgcatt agcggcattt tgactgcaag
     9001 cctgcccaca ttgtaaggat ggaagccgtc cacgtcctta tccggcgaaa tacgttccaa
     9061 aaccgcctgg ctgtcgaggt gcttcggcag tggcagctga accagaatac cgtccacttc
     9121 ggaatcgaca ttcaggcggt cgaccagtgc cagcagttct tcctgcgatg ttgattcggg
     9181 cagctcgtaa gacagtgatt tgatgccgca tttttggcag gaggttttct tgttgcggac
     9241 ataaaccgcg ctggcagggt cgcctccgac caaaaccacg gccaggcaag gcgtgtgcag
     9301 attgttctgt tggcgttgcg ccaccgcttc ggcaaccgcc tgcaggcgtt tttgcgaaac
     9361 ttctttacca ttgatcagtt gtgccgacat atccgtcctt ttgttaaaca ggtagaaaat
     9421 gctgaaaatt atcgcatttt ccgtttgttt ttacagcagg atgccgaaaa taaatttgcc
     9481 ctgaaataaa atacggctgt ttggacggat taaatatttt ttcaaatcaa aaaactatat
     9541 ttttcatctt ggtgtcttga aaataaatgt tgacgcatct ttccctgagt tgtatagttc
     9601 gcttcttcaa gtcggggcgt agcgcagccc ggttagcgca tctgctttgg gagcagaggg
     9661 tcgtgagttc gaatcccacc gccccgacca atcaatcagc acgaatcggc atgagcgccc
     9721 gtagctcaac cggatagagc accgaccttc taagtcgggg gttacaggtt cgattcctgt
     9781 cgggcgtgcc aagaatttga ttgaaaaccg tttttatggt ggctgtagct cagttggtag
     9841 agccccggat tgtgattccg gttgtcgtgg gttcgagccc catcagccac cccagataaa
     9901 ggcccgtact tttcggtacg ggtcttttcc cgtttgcgcg tactgtccgc gccgccggct
     9961 ttgcctttcg catatgcttc tgtatcatgt gggtttttct gtcttttatg gtttttgtcg
    10021 gaggctttga tggttgacgc ttggtctgtc cctgattttg aatcgaaaat ctgtccgccc
    10081 gaggcattgt ccgaacggtt ggctcggttg ccgcgcccgc tggtgtttac caacggctgt
    10141 ttcgacatcc tccacagggg gcacgttact tatctggcgc aggcgcgttc ggcaggggcg
    10201 gcgttggtgc tggcgttgaa taccgatgct tcggtgcggc gtttgggcaa ggacggcgac
    10261 cgcccgatta atcctttgga gaaccgtgcc gccgttgccg ccgctttgga aagtgtggat
    10321 ttggtaacgt ggtttgacgg ggatacgccg gcggcgttga ttgaggcggt caaacctgag
    10381 attttggtca agggcggcga ttgggctgcg gataagattg tcggtgcggc agaaacgttg
    10441 gcgcgcggcg gtcaggtgtt ttcaattccg tttctgcacc agacttcgac aacgaagact
    10501 ttggcaaaaa tccgtgcggc agagggcgga aaatgacggt tttgaagctt tcgcactggc
    10561 gggtgttggc ggagcttgcc gacggtttgc cgcaacacgt ctcgcaactg gcgcgtatgg
    10621 cggatatgaa gccgcagcag ctcaacggtt tttggcagca gatgccggcg cacatacgcg
    10681 ggctgttgcg ccaacacgac ggctattggc ggctggtgcg cccattggcg gttttcgatg
    10741 ccgaaggttt gcgcgagctg ggggaaaggt cgggttttca gacggcattg aagcacgagt
    10801 gcgcgtccag caacgacgag atactggaat tggcgcggat tgcgccggac aaggcgcaca
    10861 aaaccatatg tgtgacccac ctgcaaagta agggcagggg gcggcagggg cggaagtggt
    10921 cgcaccgttt gggcgagtgt ctgatgttca gttttggctg ggtgtttgac cggccgcagt
    10981 atgagttggg ttcgctgtcg cctgttgcgg cagtggcgtg tcggcgcgcc ttgtcgcgtt
    11041 taggtttgga tgtgcagatt aagtggccca atgatttggt tgtcggacgc gacaaattgg
    11101 gcggcattct gattgaaacg gtcaggacgg gcggcaaaac ggttgccgtg gtcggtatcg
    11161 gcatcaattt tgtcctgccc aaggaagtag aaaatgccgc ttccgtgcaa tcgctgtttc
    11221 agacggcatc gcggcggggc aatgccgatg ccgccgtgtt gctggaaacg ctgttggcgg
    11281 aacttgatgc ggtgttgttg caatatgcgc gggacggatt tgcgcctttt gtggcggaat
    11341 atcaggctgc caaccgcgac cacggcaagg cggtattgct gttgcgcgac ggcgaaaccg
    11401 tgttcgaagg cacggttaaa ggcgtggacg gacaaggcgt tctgcacttg gaaacggcag
    11461 agggcaaaca gacggtcgtc agcggcgaaa tcagcctgcg gtccgacgac aggccggttt
    11521 ccgtgccgaa gcggcgggat tcggaacgtt ttctgctgtt ggacggcggc aacagccggc
    11581 tcaagtgggc gtgggtggaa aacggcacgt tcgcaaccgt cggtagcgcg ccgtaccgcg
    11641 atttgtcgcc tttgggcgcg gagtgggcgg aaaaggtgga tggaaatgtc cgcatcgtcg
    11701 gttgcgccgt gtgcggagaa ttcaaaaagg cacaagtgca ggaacagctc gcccgaaaaa
    11761 tcgagtggct gccgtcttcc gcacaggctt tgggcatacg caaccactac cgccaccccg
    11821 aagaacacgg ttccgaccgc tggttcaacg ccttgggcag ccgccgcttc agccgcaacg
    11881 cctgcgtcgt cgtcagttgc ggcacggcgg taacggttga cgcgctcacc gatgacggac
    11941 attatctcgg gggaaccatc atgcccggtt tccacctgat gaaagaatcg ctcgccgtcc
    12001 gaaccgccaa cctcaaccgg cacgccggta agcgttatcc tttcccgacc acaacgggca
    12061 atgccgtcgc cagcggcatg atggatgcgg tttgcggctc ggttatgatg atgcacgggc
    12121 gtttgaaaga aaaaaccggg gcgggcaagc ctgtcgatgt catcattacc ggcggcggcg
    12181 cggcaaaagt tgccgaagcc ctgccgcctg catttttggc ggaaaatacc gtgcgcgtgg
    12241 cggacaacct cgtcattcac gggctgctga acctgattgc cgccgaaggc ggggaatcgg
    12301 aacatactta aaaaatacac cctttttcaa cacggggcgg tatccgtccg aatccgtccg
    12361 gattctgccg cccgacagac tgccgcccag gcgggcgggg tttcaaacat tgaggaaatt
    12421 ctaatgaaat ggctcttcac ccttttggcg gtactcaata ttgccgtatt cggcggcacg
    12481 gtgggctata aattggccgt taaagcgacg ggcggcgtgc cggaaaaccg ggccgtcgaa
    12541 aacacgcccc ccgcaacgcc cgccgccgac aatgcggcgg catccgtgga ggatacggcg
    12601 gcactgctca aaccgggcga catcctgacc gaagagcagg ccgagcagtt gcgcttgaaa
    12661 aaggaagcgg aacagaaaaa actgagggag aaaaaacagc gcgaggaaaa agcccgccgc
    12721 gaaaaactcg ccgccgaaaa ggcgcaggcg gaacgcgaaa acggcgcggc ggatgcctta
    12781 tgcgccgcgc aggcaagcct cacgatggac gaagacgact accaccgcat caaagggctt
    12841 ttgggcaaat ggtcgcacgt tgccagcagg agcgtcgaaa aacgcaccgc caaagccaaa
    12901 cctgccgaca aaacctaccg cgtcgtcctg cccgtttccg ccgatgccga aaatcaggcg
    12961 gcggagctgt ctgccaaagg tttcaacccc ataccgtttg acggcgcatt gagtttgggt
    13021 gtcggcaaca gccgggaaaa cgcccaagcc ctgcaaaacc ggcttgccgg cgccggattc
    13081 ggcggggcgc atattgtcga acactttgcc gaagccgaca ggcaggacga ttctttgagc
    13141 gtgtcgcgta tgacggtttt gtttaccggc gtgaatgccg ccgatgcgga cgaaattcgt
    13201 aaaatcacgt ccctatacgg caaactgaac ctcaagtctt gcaaataggc gcgaaagccg
    13261 gacgataaac gccaaaccca ccgcgaaggt gggtttggcg tttcggtttt gagtatctga
    13321 tatataaaaa gggaatgtat gtctcgtata tgccgactcc aagtgtgaaa gtgaattgtg
    13381 agggaataca gtgtttaatg gttcggaatg gacgacttcc gagtggtttt gattatacgc
    13441 taataataat aattatcaat aaaaatttaa aaataaatgc ggattttttg taatacgatg
    13501 aaaaataaac aaattaacct gtccgccgat gtttgtgatt cgggtcggca aactttcatt
    13561 gtgtcggaca aatcggggtc ggcagtcata gctgagggct tcgtgcctga cggaaataag
    13621 cctgccgtgt ttgaacatcc gtccgatttg gcgcggacag ggttgtcatt cgggggtgtt
    13681 tgagcgtgga ttcggggatg caaaagcggt aaaaccgttg cggatgaagg cttacggacg
    13741 gctttaggta tttttaagtg tattcaaaca acaacaaaac gcttcttcta ttgccgtatt
    13801 tagggcttgc gcggcagcct gcacaccgtg gaggccgagt gtttcgttgg ttgcggcatc
    13861 gggacgggcg gcaaggtttt ccggccggtt tttccatttt ttccgacaga gccgtgatgc
    13921 ttgggaatgg cggtttgccc gtttcagacc gccgccgttt tgggctgcga tgccgtctga
    13981 accgttcaga cggcattttt gccgcctttt tcaatattcc gccgaatgcc aaaacggttg
    14041 tccgactgtc ggcgtgctgg cttgcgcttt gtcgcgtgct tcgaccggtc cggcttcaaa
    14101 tgccagccgt ccggattcga cggcgagtgc gaaggcgcgt gccatattga ccggatcgcc
    14161 gctgcgggaa acggcagtat tcaaaagcac gccgtcaaag ccccattcca tcacttgtgc
    14221 cgcctgtgag ggcaaaccca agcccgcgtc gataatcagc ggcgtgtcgg gcaggcgttc
    14281 gcgcaggacg ttcaacgcgt aggcgtgaac cgcgcccaaa cccgtgccga tcggggccgc
    14341 ccacggcatc aacgcctgac agcccgcgtc gagcaggcgg cggcaggcaa tcaggtcttc
    14401 ggtgcaataa ggcagcactt tgaagccgtc tttaatcagg atttccgccg cttcgacaag
    14461 ctggaacaca tccggctgca aggtgtcgtc atctccgatg agttccaatt ttatccaatc
    14521 ggtttcaaac acttcgcgcg ccatttgcgc cgtcgttacc gcttcctgca cgctttggca
    14581 gcctgccgtg ttcggcagga cgggaacgcc ggtttcttga agcagcgacc aaaacccctg
    14641 accgtgcgcc tcgccgccgc ttcccgcgcg gcgcagcgag acggtaatca tcgcaggctg
    14701 ggcggtttgg atggattgtt tgaggatttc gggggtcggg taggcagccg tgccgagcag
    14761 cagccgtgaa gggaaagttt ctccgtatag ggtgagcata atgggttcct ttgtatagtg
    14821 gattaacaaa aaccagtacg gcgttgcctc gccttagctc aaagaaaacg attctctaag
    14881 gtgctgaagc accaagtgaa tcggttccgt actatccgta ctgtctgcgg cttcgtcgcc
    14941 ttgtcctgat ttaaagttaa tccaccataa cgttgttttg gggacagggg tcgtctgaaa
    15001 aggcaaaacc gcctagccgc cgacgacggg gcggacgata tcgattttgt cgttttcgtt
    15061 caaaaccgtt tccgcatacg cgcctttggg gatgaaaacg gtgttgaccg ccacggcaaa
    15121 gggctgttgc ggcgcggttt gggcgatgag gtcggcaacg cttgtgccgt gaagttcggc
    15181 gggtccgccg tttaagatga tgttcatggt ttctcgatgt ttct
//
LOCUS       DAWFAD010000040        14932 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWFAD010000040 DAWFAD010000000
VERSION     DAWFAD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14932
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.040"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="WGO49_000910"
     CDS             complement(104..1891)
                     /gene="lpdA"
                     /locus_tag="WGO49_000910"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0306033.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGAAASPKAEAPVAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKNGVVSRLTGGLAGMAK
                     GRKVDVIQGDGQFLDSHHLEVSLTAGDAYELAAPTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1968..3569)
                     /gene="aceF"
                     /locus_tag="WGO49_000911"
     CDS             complement(1968..3569)
                     /gene="aceF"
                     /locus_tag="WGO49_000911"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ0306034.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETAGSAAAAPAPAAQAAAPAPAAAPVPAAAAAPAPSAP
                     AAAKIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIMGDDIKAFVKSVMQGG
                     AAKPAAASASLGGGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVT
                     VHEEADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLV
                     LKNYFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGAC
                     FTISSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRV
                     IDGAAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3719..6382)
                     /gene="aceE"
                     /locus_tag="WGO49_000912"
     CDS             complement(3719..6382)
                     /gene="aceE"
                     /locus_tag="WGO49_000912"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ0306035.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7076..8788
                     /locus_tag="WGO49_000913"
     CDS             7076..8788
                     /locus_tag="WGO49_000913"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ0306036.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDAYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERTAAQAELTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVESENEGEIVLLLLRGDHEFNDIKAEKLAGVKSPLTMASSAAIVEQFGANGG
                     SLGPVGFKGKVYADFATEKGADWVIGANEDGYHYTSFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     LLNG"
     gene            8851..9507
                     /locus_tag="WGO49_000914"
     CDS             8851..9507
                     /locus_tag="WGO49_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ0306037.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9517..9972)
                     /gene="ruvX"
                     /locus_tag="WGO49_000915"
     CDS             complement(9517..9972)
                     /gene="ruvX"
                     /locus_tag="WGO49_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ0306038.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9965..10513)
                     /locus_tag="WGO49_000916"
     CDS             complement(9965..10513)
                     /locus_tag="WGO49_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ0306039.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10515..11075)
                     /locus_tag="WGO49_000917"
     CDS             complement(10515..11075)
                     /locus_tag="WGO49_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ0306040.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11403..13223
                     /locus_tag="WGO49_000918"
     CDS             11403..13223
                     /locus_tag="WGO49_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0306041.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSVRKGHEGD
                     KKSKAEHKKAHGKPVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLK
                     TEVAATKAQISRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKD
                     LEKQQKALAVQEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDA
                     RKLLEQKGNEQQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKA
                     EARRAEMSNLTAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGD
                     VWKGVFYSTAPATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKG
                     YMVAAGSKIGSSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13350..14834
                     /locus_tag="WGO49_000919"
     CDS             13350..14834
                     /locus_tag="WGO49_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ0306042.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAEQAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLNGAVGVSAAFLPPEAVVVSTKGRDG
                     KDRMVLKAVPEDYVYGMGGDPLAGIPAELKTVPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3556 a         3705 c         4198 g         3473 t
ORIGIN      
        1 ccggaagaaa tattttaatc cgctgtaaaa tagaaaatgc cgtctgaaca tttcagacgg
       61 catttgctca aataaacctt atcggctgtt tattcagtcg gatttatttt ttcttttgcg
      121 gaggcaggtc ggtacaagta cccaatgcca cttccgcagc cataccgatg gattcgccca
      181 aggtcgggtg cgggtggatg gttttgccga tgtctgccgc gtcgcagccc atttcgatgg
      241 caaggcagac ttcgccgatc atgtcgccac cgttcggacc gacaatgccg ccgccgatga
      301 tgcggccggt ttcggcatcg aaaatcagct tggtaaagcc gttgtcgcaa ccgttggcaa
      361 tcgcacggcc ggaagccgcc cacgggaagt tggctttggt gattttgcgg ccggatgctt
      421 tggcggacaa ctcggtttcg cccacccacg ccacttcggg ggaagtgtag gcaacgcccg
      481 gaatcacgcg cgcgtcgaag taggctttgt ggccggcgca gttttcggcg gcaacgtggc
      541 cttcgtgaac ggctttgtgc gccaacatcg gctgaccgac gatgtcgccg atggcgtaga
      601 tgtgcggcac attggtacgc atttgtttgt ccacttcgat gaagccgcga tcggttacgg
      661 caacgcctgc tttttccgcg ctgatgagtt tgccgttggg cgcgcggccg gcggcaacca
      721 atacggcatc gtagcgttgc ggctctttag gcgcatttgc accttcaaag gtaacgtaaa
      781 cgccgtcttc tttcggctcg actgcaacgg ttttggtgtt gaccataatg ttgtcaaaac
      841 ggtattcgtt ttgtttttgc catactttta ccaaatcgcg gtctgcgcct tgcatcaggc
      901 cgtccatcat ttcaaccaca tccaaacgcg aacccagcgt gctgtaaacc gtacccatct
      961 cgagaccgat aataccgccg ccgataatca gcagtttgcc cggtacttct ttcagagcca
     1021 atgcgccgct ggaatcgatg atgcgcggat cttcaggaat gaaaggcagt ttggtaacgc
     1081 ggctgcctgc tgcaatgata cagtttttga aggcaacgat ttttttctcg ccggtagggg
     1141 ctgccagttc gtacgcgtcg ccggcagtca gcgatacctc caagtggtgc gaatctaaga
     1201 attggccgtc gccttggata acatccactt tacggccttt cgccatacct gccaaaccgc
     1261 cggtgaggcg ggaaaccacg ccgtttttgt aggcgcgcag catatcgatg tcgagttcag
     1321 gctcggggta tttgataccg ttggcagcca agtggcgcac ttcgtcgata acggcggcat
     1381 tgtgcaacag ggctttggaa gggatacagc cgacgttcag gcaaacgccg cccaaggttt
     1441 tataacgttc gacgatggcg actttcaagc cttcatcggc agcagcaaat gcagcggagt
     1501 aaccgccggg accgccgccc aatacgacca cgtcgtactc ggcatcggca gaaccgccga
     1561 attgcgcggc ttgcggagcg ggagcggcag ctttgggtgc ttcttgcgca ggggcagcaa
     1621 caggtgcttc ggctttagga gaggcagccg cgccttcggc ttcaacgact acaatcaagc
     1681 cgccttcgga gattttgtcg ccgactttga ctttcacttc tttgaccacg cccgcaactt
     1741 cggcaggcac gtccatcgtc gctttgtcgg tttccaaggt aatcagggtg tcgtcaacgg
     1801 cgatggtatc gcccacattc acttcaaccg cgataatatc tacattttcg tgtccgccaa
     1861 tgtcgggcac tttcaattca actaagctca tgtctcatct ttctgaatga tgttcaaact
     1921 tccttttcag acggcacagg ccgctatgcc gtctgaagcc gatcgggtta cagggtaatg
     1981 cggcggaagt ctttcaacag cttcgccaag aatacggtga agcgcatacc ggccgcaccg
     2041 tcgatgacac ggtggtcgaa ggacaggctc aacgggcaca tcaggcgcgg ggcaaactct
     2101 ttgccgttcc aaacaggttt gatttgggat ttgcacacgc ccaagatggc gacttcggga
     2161 gcgttcacaa ttggcgtgaa gcctgtgccg ccgatgccgc ctaagctgga aatggtaaag
     2221 cacgcgcctt gcatttcttg cggtttgagc ttgccttcac gggctttttt ggacaattcg
     2281 gtcaattctt ggctgatttg tttcaagcct ttttgatcca cgtctttgat gacgggaaca
     2341 accaagccgt tcggcgtatc ggctgcgaaa ccgatgttga agtagttttt cagcaccagg
     2401 ttgtcgccgt ccagtgaggc gttgaattcg gggaatgctt tcaacgcgga aacagaggct
     2461 ttgatgatga acgccaacgg ggacagtttc acgccttcgc gttcccattc tttgttcagc
     2521 tgtttgcgga attcttccag ctcggtcatg tccgcttctt cgtgtacggt aacgtgggga
     2581 atcacaaccc agttgcggga caggttttga ccggaaattt tcttaatgcg ggacaattct
     2641 ttaacttcga cattgccgaa tttggagaag tccactttag gccacggcag taagtccaga
     2701 ccgccgccca aagatgcgct ggctgcggca ggttttgccg cgccgccctg catcacggat
     2761 ttcacaaagg ctttgatgtc gtcgcccatg atacggcctt tcaagccggt gcctttgact
     2821 tggcccaaat ccacgcccaa ttcgcgcgcc agtttgcgtg cggaaggacc ggcgtgtgct
     2881 ttggcgaaag cggcctcgtc gattttggca gcggcaggtg cagaaggtgc gggcgcggcg
     2941 gcagcagccg gaaccggagc ggcagccggt gcgggtgcgg cagcttgagc ggcaggcgca
     3001 ggggctgctg cggcagagcc ggcggtttct acttcgataa ttgcagagcc ttcggacact
     3061 ttgtcgccga cttttaagaa tacggcttta acgacaccgg cagcggtaca aggtacgtcc
     3121 attgtcgctt tgtcggtttc caaagtaatc agcgtgtcgt cttcggcaac ggtgtcgccc
     3181 actttgattt caacggcgat gacatccaca tcggtatggc cgccgatatc gggaacggct
     3241 acttgaacgg ttgcaccgcc tgcggcaacg ggtgcggcag caggtgcagg ttgtgcttcg
     3301 gcagcagcag gagcggcttc ggcagcggcg gcaccggttt caacggtcaa aattacgccg
     3361 ccttcggaga ttttgtcgcc gactttgact tttacttctt tcacgacacc ggccgcatcg
     3421 gcaggcacat ccatcgtggc tttgtcggtt tccagtgtaa tcagggtgtc gtcaacggcg
     3481 atggtgtcgc ccgctttaac ttctacggcg atgatgtcga cgttttcgtg accgccgata
     3541 tcggggactt tgatttctac gatactcatt tgagttcctt taatcttgtt cagttggatg
     3601 ccgtctgaaa gctgcttcag gcggcatcgg tacggtttgt ctgctcaaac cattcgggca
     3661 ttcggttttc agacggcctg atgtcggaat ggggcaaaca gaccgtctga aacatcaatc
     3721 agcgtttcca gctaggagct gaatcggctt tgatgccgta tttctcaatg gcttgttgaa
     3781 cggtttcttt gctgactttg ccttgttccg ccaatgcgct caatgctgcc acggcaacgt
     3841 tgtagcgatc cacttcaaag aagcggcgca ggttggcgcg gctgtcggag cggccgaagc
     3901 cgtcagtacc caagacgtgg tagtcgttcg ggatgtaggc gcggatacgg tcagcatagc
     3961 tgcggatata gtcggtagcg gcaataaccg gaccgtcatg accttgcaat ttggaagtaa
     4021 cgaaaggtac tttttcggct tccagcggat gcaggcggtt gaagcgttct acctcgacag
     4081 cgtcgcggtg cagcaggttg aaggacgggc aagaccaaat gtctgcttcc acgccgaagt
     4141 cggctttcag caattcggca ccggcaatga cttcttgcag gatggtaccg gagcccatca
     4201 attgaacttt cttatcgcct ttgccgccgg ctttcagcag gtacatacct ttcaagatgt
     4261 cttgttctgc gccttcgggc atatccggat gggtgtagtt ctcgttcatc agggtgatgt
     4321 agtagaacac gtcttcatta ttggcataca tacggcgcag accgtcttgt acgatgacgg
     4381 cgacttcgta ttggaaagtc gggtcataag atacgcagtt cggaatcagg tcggcctgga
     4441 tgtggctgtg gccgtcttcg tgttgcaggc cctcgccgtt cagcgtcgta cggccggcag
     4501 taccgcccag caggaagccg cgcgcgtgca tatcgcccgc cgcccaagcc aagtcgccga
     4561 tacgttggaa accgaacata gaatagtaaa tgtagaacgg aatcatcgcg aagttgctgt
     4621 tggcgtagct ggttgcagcc gcaatccagt cggccatcgc gcccggttcg ttaatacctt
     4681 cttgcaagat ttgaccgtca acggattctt tatagaacat cagttggtct ttgtcttgag
     4741 gggtatattg ctgacctttc ggattccaaa taccgtattg gcggaacata ccttccatgc
     4801 cgaaagtacg gctttcgtcg ggaacgatag gtacgacgcg tttgccgatt tttttgtctt
     4861 tcagcaaagt ggacaggatg cggacgaatg ccatcgtggt cgagaactcg cgttcaccgc
     4921 tggatttgag ttgtgcgtcg aatgctgaca gctcgggcac ttccaatact tcctgcgtcg
     4981 gtttgcgttg cggcaggtag ccgcccaaag catcgcggcg tgcgtgcagg tatttgtatt
     5041 cttccgtatc gggggcaaaa gtcaggtaag gcagatcgcc gctttcgatt tgttcgtcgg
     5101 taaccggaat gtcaaagcgg tcgcggaatt gtttcaggga cgctttgtcc atttttttgg
     5161 cttggtgggc aacgttctga ccttcgccgg atgcgcccat accgtaacct ttaatggttt
     5221 tcgccaagat gacggtaggt ttgccgtcgg catggttcgc tgcgcggtcg taggcgttgt
     5281 acactttttg cgggtcgtgg ccgccgcggt tcaatgccca gagttgctca tcggtcatat
     5341 cggcaaccaa tgctttcagt tcgggcgtat tgaagaagtg ttcgcgcaca tacgcgccgt
     5401 ctttggattt gtaagtttgg tagtcaccgt ccaaacattc ttccatacgt tggcgcagga
     5461 taccgtcttt gtctttcgcc aagaggcggt cccagcggcg gccccaaatg actttgacga
     5521 cattccagcc ggcgccggca aagttgcctt ccaattcttg gatgattttg ccgttgccgc
     5581 gtaccggacc gtccaagcgt tgcagattgc agttgatgac gaaaatcagg ttgtccaagc
     5641 cttcgcgtgc agccagtgcg attgcacctt gagattcggg ttcgtccatt tcgccgtcgc
     5701 cgcagaaaca ccatacttta cggcctttgg tttttgccaa accacgcgat tccaagtatt
     5761 tcaggaaacg cgcctgataa atcgccatga tgggccccaa gcccatggat acggtcggga
     5821 actgccaaaa gtcgggcaag aggtgggggt gcggatagga aggcagaccg tgtccgtcca
     5881 cttcttggcg gaagttgttc agctggtctt cggtcagacg gccctcgacg aatgcgcgtg
     5941 catagatgcc cggggcgacg tgaccttgga agaagaccaa atcgccttct tcgccttcgc
     6001 ctttggcttt ccaaaagtgg ttgaaaccga cttcgtacat ggtggcggca gattggaaag
     6061 atgcgatgtg cccacccagt tccaaatctt tcttgccggc gcgcaatacg atggcggcgg
     6121 cgttccagcg cacgaatgcg cggatgcggt gttcgatgtt ttggtcgccc ggaatgcctt
     6181 tttcgttttc aaccgaaacg gtattcaaat acggtgtggt cgtgccgtgt ggcatacgta
     6241 cgcccttgtc gcggcagtat ttgaccaggt tttccaagag gtattgcgcg cgttcgccgc
     6301 cttcatattc gaggacggag cttaacgcgt ccagccactc ttgggtttcg atagggtcaa
     6361 catcgtgtaa ttgggtggac atagtatcta tcctttatgt tgagtgttta atcggcaggg
     6421 agggtgtagc caaccccttt attttcgttt gtgaaaaata ataagcaaaa acgaaaattt
     6481 ctaaatcaaa ccgaaaaaaa taatttaagt ttaatgtttt caaaaaatta taaaagtttt
     6541 ggaaacaagg gaaaacttga aaaaaatgtt cgatctgctc cacacgaaat cctatcagca
     6601 atcgggtctg ctggcaaata accatgcctg taaaagcagg ttttatactg aagttggcgt
     6661 ttggtaaggt caagtctttt tgatttcgaa atattctgtt tgattttggc gatatttttt
     6721 cggaattgtg gtatatcgaa tcattatgtc tctgtcggca attttcctat tgtagaaaat
     6781 ctatacacaa gctttgtgga taaccgtgtt gataagccac ggcttgtgtt aaaaaagtct
     6841 tacgcaacaa atgtacgctt attttgatta aaaaataggc acatttaaat ctataaaaat
     6901 catgtgctta tgttcaatat gatgtttctg gtcatgtttt tgccgtctgt tatgggattt
     6961 gtcccgcgct tggggcaggg ggtaaatttt ggaggttggt gcaaaacctg cttttttcgc
     7021 gttacaatct gcacccgatt tacttttgaa tttgcctgtt tggaaaaata tttttatgaa
     7081 agccagccaa ttctttatct ctactttaaa agaagcccct gccgaagccg cgcttgccag
     7141 ccacaagctg atgatacgcg ccggtctgat taaagccaac gcgtccggcc tttatacttg
     7201 gatgccgatg gggctgcgcg tgttacgcaa agtcgaaaac gtcgtgcgcg aggaaatggc
     7261 gcgcgcgggc agcgtggagc tgctgatgcc ggtggtgcag cctgccgagc tgtggcagga
     7321 atccggccgc tgggaatttt acggtaaaga actgctgcgc ctgaaagacc gccacgaccg
     7381 cgatttctgc atgggaccga cctgcgagga agtcatcgcc gacatcgtgc gcaaagaaat
     7441 caacagctac aaacagctgc cgaaaaactt ttaccacatc caaaccaaat tccgcgatga
     7501 agtgcgcccg cgtttcggcg tgatgcgcgc gcgcgagttc gtcatgaaag acgcttattc
     7561 cttccatgcc gactacgcct cgcttcagac gacctatgat gccatgtatg acgcttactg
     7621 ccgcatcttt acccgtctgg gcttggcatt ccgccccgtc gccgcagaca ccggcagcat
     7681 cggcggtacg ggttcgcacg agtttcaagt gttggcggaa agcggcgaag atgttattgc
     7741 atacagcgac acttccgatt acgccgccaa tatcgagttg gcaccgacct tgccgcttaa
     7801 aggcgaacgc accgctgctc aggccgagtt gaccaaagta catacaccaa atgtcaaaac
     7861 cattgagtct ttggttgaat tcctgaatat tccggttgaa caaaccctca aatccatcgt
     7921 ggtcgaaagc gaaaacgaag gcgaaatcgt cttattactg ttgcgcggcg accatgagtt
     7981 caacgacatc aaagccgaaa aactcgcagg cgtgaaatcg ccattgacga tggcaagttc
     8041 tgccgcgatt gtcgaacagt tcggcgcaaa cggcggctct cttggtcctg tcggcttcaa
     8101 aggcaaagtc tatgccgatt tcgctaccga aaaaggcgcg gactgggtca tcggtgcgaa
     8161 cgaagacggc taccactaca ccagcttcaa cttcgggcgc gatgccgccg agcctgaatt
     8221 cgtcgatttg cgtaatgtgg tcgaaggcga cgaaagcccc gacgggcaag gccgtctgaa
     8281 actcgcgcgc ggcattgaag tcggacacgt cttccagttg cgcgacaaat acacccaagc
     8341 catgaacgta agcttccttg acaacaacgg caaatcgcaa attatggaaa tgggctgcta
     8401 cggcatcggc atcacccgcg tcgttgccgc cgccatcgag cagaataacg acgaaaaagg
     8461 catcatctgg accaaagcaa tggcgccgtt tgaagtcgtt atcgtgccga tgaactacaa
     8521 aaaatcagac accgtacgcg aagccgccga caaaatctat gccgaattgc tggcggcagg
     8581 cgcggatgtg ctgctggatg accgcgacga acgcgcaggc gtattgctga acgattccga
     8641 gctgttgggt attccgcacc gcatcgtcat cggcgaccgc gctttgaaag aaggcaatgt
     8701 cgaatacgcc gaacgccgca acaacgaagc gcaggcagtt gcaatcggag aaattgttgc
     8761 gcgtgtaaca gctttattaa atgggtaagc ttggtcaaat gccgtctgaa aataatttca
     8821 gacggcattt ttatttgggg agaaagtgaa gtggcttcag tatttttaga aggagaagca
     8881 gtcgaggtcg gcaatatttt ctgtatcggc aggaactatg ccgcacatat tgaagagtta
     8941 aaaaacgaaa taccttccga acctgtggtg tttatgaagc cgtcaggcag cattttgaac
     9001 agcggcggta caatcttgct gcccgagttt agtagggatg tgcagtttga gtgcgaactc
     9061 gtgctgctgg tcggtaagga ttcagacggc acgggcgagg gtaaagatat tttgggatgt
     9121 gtcgcaggat atggtgtagg gctggacctt acggcacggg atatccaatg ccgtctgaag
     9181 gaaaaagggc tgccctggct gaaggcaaaa gggttcaggc attccgcgtg cgtgtcggat
     9241 tttgcggcgg caggcagaat cggcaatccc gagaatgtgt tgttttcatt aaaacaaaac
     9301 ggcgtattga aacagcgcgg ggataccggt ttgatgattt acccgattcg ggaaatttta
     9361 cacaaactgg cggcagatta cggtttgggg aaaggggatt tggtgtttac cggaacgccc
     9421 tccggcgtgg gcgcaatcgg tgcgggggac aatttggcat tggagctgga cggcttggtg
     9481 cgcgcctctt tcacggtagg gtgttaaccg cgccgcttaa ccctcacgcc cgttgaaaca
     9541 ttccgccgga ccgccctcga aaaaaccgtg caagatggct tgcgccgcca cttggtcgag
     9601 caccgatttg cgttttttgc cgaagacctg tgcttccgaa agcaggcttt cggcatagac
     9661 ggacgacagc cgttcgtcaa cccaatagac ggggagattg aacctgccgt tcagcctgcg
     9721 tccgaacttg cgcgacaggt gcgtcatttc atgtttcgtg ccgtcggtat gcacgggcag
     9781 tccgacgaca aaataacgcg gctgccattc ttgaaccagc ttggcgattg ccgcgaactt
     9841 ttcatcgttg ctgccgccgg taacggtgct caaaggatgg gatagcccta attccgcgtc
     9901 tccttgtgcc acgccgatac gcgcttcgcc gaagtcgaat gccagtgccg ttccttttgg
     9961 aattttatgc atggccggct cccgaaaaca gggcgagcgg gtcgatgccg agtttggcga
    10021 atgcggcggc gtaacggtgt tcgtagggga tgtcgaacag gatgtgttcg tcggcgggaa
    10081 cagtcagcca cgcattgtcg gcaagttcgc gttcgagctg gccttttccc cagcttgaat
    10141 agcctatgct gatcaaggct ttgtcaaccg caccttcgcg tgaaatattt tcaatcacgt
    10201 ctcgggaaga agttagcgcg atattgtctg aaacgccgat actgctttgc cagttgccga
    10261 tcggggtatg cacgacataa cctcgctcga cttgcaccgg cccgcccatc atcacgctgt
    10321 cgtgctgcat ccgcatgggg atgtttttgc cggtggcgga aaaaatcatg tccatcgtaa
    10381 tcggagaggg tttgttgatg gcgatgccga gtgcgccgtc ttcatcgtgt ttgcagatat
    10441 agacgaccga ttgtgaaaaa aacgcgtctt ccatatcggg catggcgacg agaaagtggt
    10501 tggagaggtt catatcagcg tttgtcggga ttttctatcg ggttggaaat catgcaggcg
    10561 ccggcaagca ggttgccgga agtatcggct tgcaccccat cggtttgcgg tatgccgccg
    10621 ccgaaacagg aaaccgcgct taaggaattt ttcggcgagc cttggatgat tttatcgctg
    10681 tacaccaaat aggcgaaggt tttgcgtttg gggtcgtaat aacggacaat ctgccggctc
    10741 ttgaacgcga agctcgcacc gtgtttgaaa acttctttcg gtttgcgcac ggcggtttcg
    10801 tcaaaagaaa tcgaagatgc cgtctgaacg cacgaaaccg atgcgtcgga cgcgtcctct
    10861 tccaaattga ccatttcctt caagccgcct ttttttgcat acgaaatata acaggcaacc
    10921 ccttgaacgt cgggatcgtc gaatccttcc acttcgatac ggtcgttttt gcccagtatg
    10981 ttgaaaacgg tacttgcccg tccgatttta tcggtttcgc cgctgccgca ggcagtcagc
    11041 aggacggcgg cagacagcag tagcagtctg ttcatcaaaa tttccttatc ggttgaatcc
    11101 ccgttttccg tgaaggcggc agggcttgct ttgtttggag gggcgcacgt tcccaaaatc
    11161 gggcaacata cggtatgcgg cttaatgtgc taccctgtgt gtcggaacac aatatccgtt
    11221 gcggcattga aaatatgatg cacaatataa ggctgtttga gccgattgca agtatccgcg
    11281 caaagaaggc gcaagattgg gcaaacggca ttttcagacg gcataactga cagtataatc
    11341 caaacgtccg gctgctttcg tgcagccttc agtattatcc aactttttcc gaaggtcgaa
    11401 ccatgcgcta caaacccctt ctgcttgccc tgatgctcgt tttttccacg cccgccgttg
    11461 ccgcccacga cgcggcacac aaccgttccg ccgaagtgaa aaaacagacg aagaacaaaa
    11521 aagaacagcc cgaagcggcg gaaggcaaaa aagaaaaagg caaaaatggc gcagtgaaag
    11581 ataaaaaaac aggcggcaaa gaggcggcaa aagagggcaa agagtccaaa aaaaccgcca
    11641 aaaaccgcaa agaagcagag aaggaggcga catccaggca gtctgtgcgc aaaggacacg
    11701 aaggggataa gaaatcgaag gcggaacaca aaaaggcaca tggcaagccc gtgtccggat
    11761 ccaaagaaaa aaacgcaaaa acacagcctg aaaacaagca aggcaaaaaa gaggcaaaag
    11821 gacagggcaa tccgcgcaag ggcggcaagg cggaaaaaga cactgtttct gcaaataaaa
    11881 aagtccgttc cgacaagaac ggcaaagcag tgaaacagga caaaaaatac agggaagaga
    11941 aaaatgccaa aaccgattcc gacgaattga aagccgccgt tgccgctgcc accaatgatg
    12001 tcgaaaacaa aaaagccctg ctcaaacaaa gcgaaggaat gctgcttcat gtcagcaatt
    12061 ccctcaaaca gcttcaggaa gagcgtatcc gccaagagcg tatccgccaa gagcgtatcc
    12121 gccaagagcg tatccgtcag gcgcgcggca accttgcttc cgtcaaccgc aaacagcgcg
    12181 aggcttggga caagttccaa aaactcaata ccgagctgaa ccgtttgaaa acggaagtcg
    12241 ccgctacgaa agcgcagatt tcccgtttcg tatcggggaa ctataaaaac agccagccga
    12301 atgcggttgc cctgttcctg aaaaacgccg aaccgggtca gaaaaaccgc tttttgcgtt
    12361 atacgcgtta tgtaaacgcc tccaatcggg aagttgtcaa ggatttggaa aaacagcaga
    12421 aggctttggc ggtacaagag cagaaaatca acaatgagct tgcccgtttg aagaaaattc
    12481 aggcaaacgt gcaatctctg ctgaaaaaac agggtgtaac cgatgcggcg gaacagacgg
    12541 aaagccgcag acagaatgcc aaaatcgcca aagatgcccg aaaactgctg gaacagaaag
    12601 ggaacgagca gcagctgaac aagctcttga gcaatttgga gaagaaaaag gccgaacacc
    12661 gcattcagga tgcggaagca aaaagaaaat tggctgaagc cagactggcg gcagccgaaa
    12721 aagccagaaa agaagcggcg cagcagaagg ctgaagcacg acgtgcggaa atgtccaacc
    12781 tgaccgccga agacaggaac atccaagcgc cttcggttat gggtatcggc agtgccgacg
    12841 gtttcagccg catgcaagga cgtttgaaaa aaccggttga cggtgtgccg accggacttt
    12901 tcgggcagaa ccggagcggc ggcgatgttt ggaaaggcgt gttctattcc actgcaccgg
    12961 caacggttga aagcattgcg ccgggaacgg taagctatgc ggacgagttg gacggctacg
    13021 gcaaagtggt cgtggtcgat cacggcgaga actacatcag catctatgcc ggtttgagcg
    13081 aaatttccgt cggcaagggt tatatggtcg cggcaggaag caaaatcggc tcgagcgggt
    13141 cgctgccaga cggggaagag gggctttacc tgcaaatacg ttatcaaggt caggtattga
    13201 acccttcgag ctggatacgt tgatcggatt gccgaaacgg taggctttat gcaaatcgtg
    13261 ccgatatggt gcgctgttcc gtgaagtttg ccgtttgccg tacctgtatt ttccggtttt
    13321 tggaaatcaa gagagaaagt taaagaagaa tgtcgaaacc tgtttttaag aaaatcgcac
    13381 tttatacttt gggtgcaatc agcggcgtgg ccgtcagtct ggcggtgcag ggttttgccg
    13441 ccgagaagga caggcgggat aacgaagtcc tgccggtgca atccatccgc acaatggcgg
    13501 aggtttacgg tcaaatcaag gcaaactact atcaggacaa acccgatgcc gatttgtttg
    13561 aaggtgcgat gaagggtatg gtggccggtt tggatccgca ttccgaatat atggataaaa
    13621 aaggttatgc cgagataaag gagtccacca gcggcgaatt tggcggcttg gggatggaaa
    13681 tcgggcagga agacggattt gtcaaagtgg tttcgccgat tgaggacacg cctgcggaac
    13741 aggcgggggt gaaaagcggc gatttcattg tgaaaatcga taatgtttcg acacgcggca
    13801 tgacggtcag cgaagcggtg aagaaaatgc ggggcaagcc gggtacgaag attactttga
    13861 cgctgtcgcg caaaaatgcc gacaagccga tagtcgtcaa cctgacccgt gccattatta
    13921 aagtgaaaag cgtccgccat cacctgatcg aacccgatta cggctatatc cgcgtgtcgc
    13981 agttccaaga gcggacggtc gaaagcgtca ataccgccgc aaaagagctg gtaaaggaaa
    14041 ataaaggaaa accgctcaag gggctggtgt tggatttgcg cgacgacccc ggcgggcttt
    14101 tgaacggcgc ggtcggcgtg tcggcggcgt ttttgccgcc tgaagcagtc gtcgtcagca
    14161 ccaagggacg cgacggcaaa gaccgcatgg tactgaaagc cgttcctgag gattatgtgt
    14221 acgggatggg cggcgatccg ttggcgggca ttcctgccga gttgaaaacc gttccgatga
    14281 cggtgttgat taattccggt tcggcttccg cgtcggagat tgtcgcaggt gcattgcagg
    14341 atcacaaacg cgcggtcatc gtcggtacgc agagcttcgg caaaggttcg gttcagactt
    14401 tgattccttt gtccaacggc agcgcggtca agctgacaac ggcactgtat tatacgccga
    14461 acgaccgttc tattcaggcg caggggattg ttcccgatgt cgaagtaaaa gataaggaac
    14521 gcatttttga aagccgcgag gcggatttgg tcggacacat cggcaatcct ttgggcggcg
    14581 aggatgtgaa cagtgaaacc cttgccgtgc cgcttgaaaa agatgcggat aagcccgctg
    14641 taaaagaaaa aggtaaaaag aaaaaggacg aggatttgtc ttcaaggcgg attcccaacc
    14701 ctgccaaaga cgaccagttg cggaaagctt tggatttagt caagtcgccc gagcagtggc
    14761 agaagtcttt ggggctggcg gcgaaaaagc cggtttcaaa taaagataag aaagataaaa
    14821 aagataagaa gtaggttttg acgatgccgt ctgaaaggat ttcagacggc atattatgtt
    14881 tgaaaggaca ggttatgagc gattttttac cggaatttga accgtgcggc at
//
LOCUS       DAWFAD010000041        14902 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWFAD010000041 DAWFAD010000000
VERSION     DAWFAD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.041"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(87..3572)
                     /gene="smc"
                     /locus_tag="WGO49_000920"
     CDS             complement(87..3572)
                     /gene="smc"
                     /locus_tag="WGO49_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ0306043.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATEQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQLQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDHAAAPQLWQHITAPAEWQHALSVILAERLHARAVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHYWLDGVLCAPDLSYALAHQNDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELENLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHTSDGLSDDIVTLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYRQQIAQLEQQTLDWQERQQELVLAYETEFQNDEQHIKLDELT
                     ESVHTLDEEYIAVQEKLTQIQEQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3574..3825
                     /locus_tag="WGO49_000921"
     CDS             3574..3825
                     /locus_tag="WGO49_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306044.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            3822..4544
                     /gene="lpxH"
                     /locus_tag="WGO49_000922"
     CDS             3822..4544
                     /gene="lpxH"
                     /locus_tag="WGO49_000922"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ0306045.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            4813..6399
                     /locus_tag="WGO49_000923"
     CDS             4813..6399
                     /locus_tag="WGO49_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0306046.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6567..6770)
                     /locus_tag="WGO49_000924"
     CDS             complement(6567..6770)
                     /locus_tag="WGO49_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ0306047.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6886..7383
                     /locus_tag="WGO49_000925"
     CDS             6886..7383
                     /locus_tag="WGO49_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ0306048.1"
                     /translation="MTALTLPEDIRQQEPSALLYPLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7465..8658
                     /locus_tag="WGO49_000926"
     CDS             7465..8658
                     /locus_tag="WGO49_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0306049.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEATAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9061..9996)
                     /gene="hemC"
                     /locus_tag="WGO49_000927"
     CDS             complement(9061..9996)
                     /gene="hemC"
                     /locus_tag="WGO49_000927"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ0306050.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYARLEEMPKGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHDTTHACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLQADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10115..10630
                     /gene="ybeY"
                     /locus_tag="WGO49_000928"
     CDS             10115..10630
                     /gene="ybeY"
                     /locus_tag="WGO49_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ0306051.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGEILPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMLAA
                     GFPNPYQEDEY"
     gene            10632..11456
                     /locus_tag="WGO49_000929"
     CDS             10632..11456
                     /locus_tag="WGO49_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEZ0306052.1"
                     /translation="MDGTQSKPKFFERLISRLAGEPDSAEDVLTLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11585..12964)
                     /gene="nhaC"
                     /locus_tag="WGO49_000930"
     CDS             complement(11585..12964)
                     /gene="nhaC"
                     /locus_tag="WGO49_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ0306053.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPNVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVILALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13255..14478)
                     /locus_tag="WGO49_000931"
     CDS             complement(13255..14478)
                     /locus_tag="WGO49_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ0306054.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTGVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAIAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3515 a         4198 c         3831 g         3358 t
ORIGIN      
        1 tttaaattta attcactata ccgtctgaaa acctgtatcg caacacgtca ttaaaaacgg
       61 caagattcag ccgttctgca acccgttcaa acggcttccg ccatttccaa cgcctgcttg
      121 atgtccacgg cgacgacgcg cgatacgcct ttttcctgca tggttacgcc gaccagctgc
      181 tctgccattt ccatggtcag gcggttgtgg gagatgtaga ggaactgggt ttgcgccgac
      241 atttctttga ccaggttgca gaaacgcgag gtgttggcgt cgtccagcgg ggcatcgacc
      301 tcgtccaaaa ggcagaacgg agcggggttg aggctgaaca gagcgaacac gaggctcatg
      361 gcggtgaggg ctttttcgcc gccggagagg aggtggatgg tgctgttttt cttgccgggc
      421 ggacgcgcca taatggacac accggcggtc agtaggtcgt cgcctatcat tttgagagtg
      481 gcttcgccgc cgccgaacag ggtcgggaag aaggtttgga ctttgccgtt gacggcgtcg
      541 aaggtttctt tgaaacgcgc tttggttttg tcgtcgattt gggcgatggc ttcttccaaa
      601 atggtgatgg cggcctgcac gtcttcgctt tggctgcggt agtagccgtc gcgttcgcgc
      661 gcttcttcga gttcttgcag ggcggcgagg ttgaccgcgc cgagggcttc gatttgttgc
      721 gagaggctgc cgatgctgct gttcaatact ttcggcgatt ctttcgccaa cgcttcgagc
      781 gcatccaaat cggcggcgcg ttcggtcagg ttttgatggt agcgtttggc gttgatcagg
      841 gcttcctgtt gttgcaacag ggcggtttgg gtggcggcct gaagctgtgg caactgggtt
      901 tgcagggttt gtacgcgggc gtattgttcc ctaccctgct cctgaatctg cgtgagtttt
      961 tcctgcacgg cgatgtattc ttcgtccagc gtgtgtacgg attcggtcag ctcgtcgagc
     1021 ttgatgtgct gctcgtcgtt ttggaactcg gtttcatagg cgaggacaag ctcttgctgg
     1081 cgttcctgcc agtcgagggt ttgctgttca agctgggcga tttgctgccg gtagttttgt
     1141 ttttgctggt tgagtttgtg gacggcgact tcggcaagcc catattggcg gttggcttcc
     1201 aacagggcaa gctgcgcctg tttcagacgg ccttgctgct cttggcggct gtgcgcggtg
     1261 gtttgctgct ggtgttcgag ttcggcggcg gcttcctgca aggtaacgat gtcgtctgaa
     1321 agcccgtcgg acgtgtgttg caacacggtc tgttcttccg ccaactgcgc cagttcgcgc
     1381 tcgatgtgtt cgcggcggat ttgcccttgg ttggtacgcg ctaagagttc ggcggcgcgt
     1441 tgctgcgcct gactgtattg gcgcgtgtgc tgctgttgct gctgcatcag gtttttatgt
     1501 tgcacttcag acgagcgcac ggcagcttcc gcctgtttga acgcggcttc ggcggcggaa
     1561 agttcggggg cgaggttttc cagttcggac gcgatgccgt cgaggcgcgc tttttgggca
     1621 atcaggcttt cctgcgcggg tttggcatag agcaggacgc tgactttatc gacctgatga
     1681 ccttcgggcg tgagccagat ttggtgtgcg cccaaatcgt tttgatgcgc gagggcatag
     1741 ctcaaatcgg gcgcgcacaa tacgccgtcg agccagtagt gcaatgccgt ctgaaacggc
     1801 ggctgcgctt ggatttggtt cagcaatgcc tgtacgggca gggatttttt gatgccgccg
     1861 gagagaccgt ctgaaagcca cgccgcctgc ccctgcggca aaggttcggg cggtacgaaa
     1921 ccttgcggca cggcgcgggc gtgcaggcgt tctgcaagga tgacggacaa ggcgtgctgc
     1981 cactcggcag gcgcggtgat gtgttgccac agctgcggcg cggcggcgtg gtcggttgcc
     2041 tgccagaaat cggctgcttc ctgctgttgc gacaggattt gcgacaacgc ctgctgctgc
     2101 gcctgcaagg tgatatgttg ctgcttcagg ctttggaagc ggtttgaggc cgtctgaaac
     2161 gcctcgcggg cggcgtgtaa ggcttcttcg gcggcgatga tttgttcttc gtaatgctct
     2221 tgccggcttt gcaacaaggc agcggcctcc tgcgcggcgg cggtttccgc ttcgtcgggc
     2281 aggttcaggg cttggttctc ctgtttcaga cggcctttgc gctcttcgtg cttggcaacg
     2341 gtttgttcgg catgggcaag ctgctgctgc ttcaacgcca gttcgcggcg gatgcggttt
     2401 gcctcgtcct gctgggtttg gaaggcggca ttgagcgcgg tttgggtttc ttccaattcg
     2461 ggcagacgct cttcgtgttc ggcaacctgc atcgcccact ccgccagctc ggtttgcttt
     2521 tcttcgacgt ataactcgtt ttcttcaagc tgcacgcgga tttgctgctg ctcttgatga
     2581 atacgctgca actgcgcctg cgccgcctgc ttgtcgcgtt cgatgcgttg gtgcaggttt
     2641 tgctgatggc ggatttgttc ttccaaacgg gcaatctgct cgcgcaacac gccgcgcttg
     2701 ttgctcaatt catgcaccgc ctgttgctgc gactgttcgg cggtctgcaa ggcgtgtact
     2761 tcgtcgttta acgcctgaac ctgcgcggcg gtttcgtcct gctgcgcctg caaagactga
     2821 tgctgctcgg tcgccttgtc ggcggcggca agcgattgct gccattgggc gtaatcgagc
     2881 aaatcctgtt gccggttcaa ctgcgcggtc agggatttgt agcgttcggc ggtttccgcc
     2941 tgtttctcca gcttttccac ctgacgcgcc aattcgttct gcaaatcgcc caaacgctgc
     3001 aaatgctcgc gcgtgtcttt cagacggcct tccgtctcct tgcggcgttc cttatatttg
     3061 gacacacctg ccgcctcctc gatataggcg cgcaattcct ccggccgcgc ttcgatgatg
     3121 cgcgaaatca tcccctgctc gataacggca taaccgcgcg cgcccacgcc cgtacccaaa
     3181 aataaatcgg taatgtcgcg gcggcgcacg gtctgattat tgatgaaata agtcgattcg
     3241 ccctgccgcg tcagctgccg cttgatgctc acctcggcat actgccccca cgcgccctgc
     3301 aaactgtggt cgctgttgtc aaacaccagc tccaccgaag ctcttggcgc aggacggcgc
     3361 gttgccgcac cgttaaaaat cacgtcctgc atactctcgc cgcgaagctg cttcgccgaa
     3421 gcctcgccca acacccagcg caccgcgtca atcacattcg acttgccgca gccgttgggc
     3481 ccgataaccg cgacaagctg ccccggcaca tgaattgtgg tcgggtcggt aaaagatttg
     3541 aagccggaga gtttgatatg ggtcaggcgc ataatggtcg gaaaaaataa aaaagaagcg
     3601 tattttaacg gaaatcccgc cgcaccaccc catatcttgc cggcaaaacc ttaccgcatc
     3661 ccgcaccctc gatgccgtct gaagccttca gacggcattt ttaacgcgcc cgaaccccga
     3721 ttccgccaac gcccgatgct tggcgcggca tttgccgtat tgccccgccg ccgggcgcgt
     3781 tataatccgc cacgacatcc gcccgatttg gaaacctgcc tatgaaaccc gcctatttca
     3841 tctccgacct gcatttgagc gaaaagcacc ccgaactgac cgcgctgttg ctgcgttttt
     3901 tacgttcttc cgccgccggg caggcgcggg cggtttacat tttgggcgat ttgtttgatt
     3961 tttgggtggg cgatgacgaa gtttccgagt tgaatacttc ggttgcgcgt gaaatcagga
     4021 aattgtccga caaaggcgtt gccgtgttct tcgtcagggg caaccgcgac ttcctgattg
     4081 gtcaggattt ttgccggcag gcaggcatga cgctgctgcc ggattactcg gttttggact
     4141 tgttcggctg caaaaccctc atctgccacg gcgacactct gtgtacggat gacagggcat
     4201 accaacgttt ccgcaaaatc gtgcatcgga agcggctgca aaaactgttc ctgatgctgc
     4261 ccctgaagtg gcgcacgcgc cttgccgcca aaatcaggcg cgtcagcaaa atggaaaaac
     4321 aggtcaagcc cgccgatatt atggatgtca atgccgcctt taccgcgcgg caggttcgcg
     4381 cctttaacgc ggaaaggctg atacacggac acacccaccg cgagcatatc catcacgaaa
     4441 acggctttac ccgcatcgtt ttgggcgact ggcataacga ctatgcttca atcctccgcg
     4501 tggacgggga cggcgcggta ttcgtgccgc tggaaaaatg ctgaaaatgc cgtctgaagc
     4561 ctttcgggcg gcttttttta cacgcccctt tcccacactc cctccgccgc atcatcacca
     4621 tccttaaccc gcattaatgg gcgttatcga taaaatgctt gaccgattac ataagattac
     4681 gtaaagtgtg caaacgcata acaattggtc ttaccaattt atcttttaac caaaatatcg
     4741 ggtgtacggg atttgaacgt ctttccaaac ctcgacgcgc cttatgatgc aaatgcctaa
     4801 aaaggattcg ttatggcact tttcctcagc atattcccca tcgtcctgct gatttggctg
     4861 atggtgaaaa aaaacagtat gccctcctac gtcgcgctgc cgattaccgc agtgctgatt
     4921 tacgccatca aacttttcta cttcggcgat gcgggcatgc tgctcaacgc caccgccgct
     4981 tccggcctcg tcaaaacgct cacgccgatt accgtgattt tcggcgcgat tatgttcaac
     5041 cgtatgatgg aaaccacggg ctgcattgat gtcatccgca aatggctggc gaccatcagc
     5101 cccaaccccg tagcgcaact gatgattatc ggctgggctt ttgcctttat gattgaaggc
     5161 gcatccggct tcggcacgcc tgccgcgatt gccgcgccga ttctgatgag cttgggcttc
     5221 aacccgttga aagtggcgat tttcactttg gtgatgaact ccgtccccgt ctctttcggc
     5281 gcggtcggta cgccgacctg gttcggtttc gcaccgctga acctgagtgc cgaagacatc
     5341 ctcgccatcg gcaggcagac cggcgtaatg cacttcttcg caggtttcgt catccccgtc
     5401 atcggcttgg gcttcatcgt accttggtct gaaatccgca aaaacttggg cttcgtcgcc
     5461 attgccgtct tctcctgcac cattccttat gtcgcattgg cgatggtcaa cgaagaattc
     5521 ccgtcgctcg tcgccggcgc aatcggcctg atggtgtccg tattcgccgc caaccaaggc
     5581 tggggcttga gcaaagacca cgccaaagac ccgaatgccg aaaaagtgcc gttcgcccaa
     5641 gtcgccaaag cactcgcccc tttgggtatg ctgatcggca tgctggtggt tacgcgcatc
     5701 aaacagctcg gcatcaaagg cattttgacc agcaaagaag aatggttcag cttccaactg
     5761 ccgtttgatt tgtccaaaat caccgtcagc gactccctga cgattacctt cggcaatatt
     5821 ttcggacaag atgtcagcgc gtcttaccaa acgctgtacg tcccggcttg gattccgttt
     5881 gtgctgaccg tttggatttg catcctgctg tataaaacca aattcaaaga tgcctggacg
     5941 atttattccg taactttcaa tcaaaccaaa aaaccgctgc ttgccctgat gggcgcgctg
     6001 attatggttc agctgatgct ggtcggcggc gacaattcga tggtgaaaat catcggtaag
     6061 gaatttgccg caatggcggg cgaacactgg gtttatttct cgccgtatct gggcgcgatc
     6121 ggtgcgttct tctccggctc caacaccgtg tccaacctga ctttcggccc gattcagcag
     6181 caaatcgcct tggataccgg cctgtccgtt accctgattc tggcgttgca gtccgtcggc
     6241 ggcgcgatgg gcaatatggt gtgcctcaac aacatcatcg ccgtatgtac cgtattggat
     6301 gtgaaaaatt ccgaaggtgc gattatcaag aaaaccgtta tcccgatggc gatttacggc
     6361 gtgattgccg tcgtcgcggc aatgattttc ttcctctagg cgaaacgcca ccatccatag
     6421 cggcagcacg cggggaacgg aaaccgccgt tccccgtctg ggcggcgcgt cgggcagaat
     6481 cccggtgccg gaacagcccg ccccgccaaa caaatgccgt ctgaaaccgg aaaaggcttc
     6541 agacggcatt tttccgccgt ttgcattcag gcgtaatgat gatgcggcat cttgccgtca
     6601 aggcggtagc gcgtgccgca atacgggcag gcaacgctgc ccgattctcc ttcgcacaaa
     6661 ggcaaaaaca ctctcggatg cccgttccac tgctcgttgt cgggtcccga gcaatacagc
     6721 ggcagatttt ccggcaacac ggaaatttcc tgcagattca gattgtccat ttgattttcc
     6781 tttgcgtggt tggcgtgcgc ccattttacg ccatcggcag gctaaaggat attttcggcg
     6841 caaagccgca atccgctata atccccactt ttcagacggc ataccatgac tgcgcttacc
     6901 cttccggaag acatccgcca acaagagcca tccgccctgc tctatcccct cgtttccgcc
     6961 tacctcgaac acaccgccca aaccggcgac gaatccctct cctgcctgtc cgacgaccag
     7021 cacacgctga ccgcattctg ctacctcgac agccaagtcg aagaaggcgg cttcgtgcaa
     7081 ctcatcgcct ccggttacgg cgaatatatt ttccgcaacc cgcttgccga cagcctgcgc
     7141 cgctggaaaa tcaaagccgt gccgaaagtc ttggacaaag ccaaagccct ctacgaacaa
     7201 cacggcaaaa ccatcgaaac gctcgccgac ggaggcgcag acatcgaatc cctgcgcaaa
     7261 cagttccccg actttgaaga atgggacggc gcatactacg aagccgccga acaagacctg
     7321 cccctgcttg cagaacacat acagtcaaac tgggaaacct tcgcccatat cgggcaggcg
     7381 tgattgcgtc tgtttccagc ccgtgtaaaa cagcgtaaaa tcggcaaacc cgaatgacct
     7441 tccgtcccca tcaaggagca agctatgttc ttcaagcaca tcgaagccgc ccccgccgat
     7501 ccgattctcg gtttgggcga agcattcaaa gccgaaaccc gccccgaaaa agtcaacctc
     7561 ggcatcggcg tttataaaga cgcatccggc gcgacacccc tcgtcaaagc cgtcaaagaa
     7621 gccgaaaaac gcctgttgga aagcgaaacc accaaaaact acctcaccat cgacggcgtt
     7681 gccgactaca acgcgcaaac ccaaatcctg ctgttcggca aagaccacga aatcatcgcc
     7741 agccgtcgcg ccaaaacagc gcaaagcctc ggcggtacgg gcgcattgcg tattgcggcc
     7801 gagtttgcca aacgccagtt gaacgcgcaa accatctgga tttccaatcc gacttggccc
     7861 aaccacaacg ccatcgccaa agcggtcggt atccaagaca aaccttatcg ttactatgat
     7921 gccgccaaac acggtttgga ttgggacggc atgattgagg acttgagcca agcgcaaaaa
     7981 ggcgacatcg tcctgctgca cggctgctgc cacaatccta ccggcatcga ccctacgccc
     8041 gaacaatggg aaactttggc aaaactttct gccgaaaaag gctggttgcc gctgtttgac
     8101 tttgcctacc aaggcttcgg caacggtctg gaagaagatg cctacggctt gcgcgtgttc
     8161 ttgaaacaca atacagaatt gctgattgcc agctcttatt ccaaaaactt cggtatgtac
     8221 aacgagcgcg tgggcgcgtt cactttggtg gccgaagatg aagcaaccgc cgcccgcgcc
     8281 cacagccaag tcaaaaccat catccgtacc ttgtattcca atccggcttc acacggcgcg
     8341 aacactattg cgctggtgtt gaaaaatgat gatttgaaag cacaatggat tgccgaactc
     8401 gatgaaatgc gcggccgcat caaagccatg cgccaaaaat ttgtcgagtt gctcaaagcc
     8461 aaaggtgcaa gccaagactt tgatttcatt atcgaacaaa acggtatgtt ctctttcagc
     8521 ggtctgactc ccgaacaagt tgaccgtttg aaaaacgagt ttgccattta tgccgtccgc
     8581 tccggccgca tcaacgttgc cggcattacc gacgacaaca tcgattatct gtgcgaaagc
     8641 atcgtgaaag tactgtaaaa agcacagcca tatgaaaatg ccgtctgaac cttaaccagt
     8701 tcagacggca tctgtttttt gttaaaaaac aataaactat aacaatttca aacaagctaa
     8761 aaacatctat aaaatcaacc caatatgatg aactcaaata aaaatacgaa tatcatcatt
     8821 ctcaaattca aaatttgaac gccacagtat tatccgaagc cacaaaaacc tttccgccgt
     8881 cattcccgcg caggcgggaa tccgggaaac caatgctgca agaattgatt agaaacaact
     8941 gaaaccgagc aaactggatt cccacctgcg cgggaatgac gaaataaggc gagtaaaact
     9001 gatgaaacaa attgattaaa aacaataact tatattattt tatttaaatt ttcgataaaa
     9061 tcaattttcc gtattcaata ctgctccaat caattcccgc gcaccgtcgt ccgccaattt
     9121 cttagcgaca gcgcgtccga gcgcgtcggc atattcggca ggggcttgcg cgtccgcctg
     9181 caacacaacc gaaccgtcgg ggtgtccgac cagtccgcgc aaggtcagca agccgttttc
     9241 ttccgtgcaa tatgcggcca gcggcacttg gcagcttccg cccaaagcgc gcgccaaagc
     9301 tcgttcggcc gtaacgcagg cgtgtgtggt atcgtggttt agcggtttta aaacctcata
     9361 caaatcttcg cggtgcgcgg caatttcgat acccaatgcg ccttgtccgg cggcaggcag
     9421 gctgtcggat tccgacaaaa tcatgcggat gcgtccgtcc aatttcagac gctgcaaacc
     9481 ggcggcggcc aaaataattg cgtcgtattc gccgttatcg agtttggaca aacgggtttg
     9541 cacattgccg cgcaaaggtt tgataagcaa atgcggatag cgcgcgcgca actgggcttc
     9601 gcggcgcagg ctggatgtac cgacgacagc gcctttgggc atttcttcca aacgcgcgta
     9661 ttggttggac acaaacgcgt caaacggatt ggcgcgttcg ccgatggcgg caagcgcgaa
     9721 accttcaggc aaatccatcg gcacgtcctt aatcgaatgc accgccaaat cggcgcgccc
     9781 gtcatataaa gcctgttcca actctttgac aaacaagcct ttaccgccga cttttgacaa
     9841 agttttgtcc aaaatctgat cgccgcgcgt ggtcatgccc aaaatctcga cttcgcaatc
     9901 gggatacaga gccttcagac ggccttggat atgctttgcc tgccacatag caagcaggct
     9961 ttcgcggctg gcgatgacga gttttttcgg gttcatgttc aatgcgttca aagaatggaa
    10021 acggcgtgaa gtctatcata ttttgaacgt ttcttcagac ggcaccgtca ggtctatgtt
    10081 atatttacac ccacaaaaca cgaaacccgc cacaatgaaa cgcaccaaaa aatacccttt
    10141 cttaacgttg cagcggcaac gtttccattt gaactttgaa aacgcctctt ccgccgccgg
    10201 catcccagcc gaacgcgatt tctaccgctg ggcgtggtct gccttgaaaa atgaataccg
    10261 ccgcgccgac atcagcctga ttcttctgga cgaagaagaa gcccgagcct acaaccgcga
    10321 ctaccgcggc aaagattacg ccaccaatgt attgagtttc gcgctcaacg aaggcgaaat
    10381 cctgccctgc caagtttcgg aaaaactgta cggcgatttg attatctgcc cgcaagtggt
    10441 tttgaaagaa gccgccgaac agggcaaaac gcccgaacgg cattttgccc acctgaccat
    10501 acacggcgtt ttgcacctga tgggctacga ccacatcgaa gacgatgaag ccgaaataat
    10561 ggaagccgaa gaaatccgcc tgatgctggc ggcaggcttc cccaacccct accaagagga
    10621 cgaatattaa aatggacggt acgcagtcga aacccaagtt tttcgaacgc ctgatctccc
    10681 gacttgccgg cgagcccgac tccgccgaag acgtattgac cctgctgcgc caagcgcacg
    10741 agcaggaagt ttttgatgcg gatacgcttt taaggttgga aaaagtcctc gatttttccg
    10801 atttggaagt gcgcgacgcg atgattacgc gcagccgtat gaacgtttta aaagaaaacg
    10861 acagcatcga acgcatcacc gcctacgtta tcgataccgc ccattcgcgc ttccccgtca
    10921 tcggtgaaga caaagacgaa gttttgggta ttttgcacgc caaagacctg ctcaaatata
    10981 tgttcaaccc cgagcagttc cacctcaaat cgatattgcg ccctgccgtc ttcgtccccg
    11041 aaggcaaatc gctgaccgcc cttttaaaag agttccgcga acagcgcaac catatggcga
    11101 tcgtcatcga cgaatacggc ggcacgtcgg gtttggtcac ctttgaagac atcatcgagc
    11161 aaatcgtcgg cgaaatcgaa gacgagtttg acgaagacga tagcgccgac aatatccacg
    11221 ccgtttcttc cgaacgctgg cgcatccatg cagctaccga aatcgaagac atcaacacct
    11281 tcttcggcac ggaatacagc agcgaagaag ccgacaccat tggcggcctg gtcattcaag
    11341 agttgggaca tctgcccgtg cgcggcgaaa aagtccttat cggcggtttg cagttcaccg
    11401 tcgcacgcgc cgacaaccgc cgcctgcata cgctgatggc gacccgcgtg aagtaagcac
    11461 cgccgtttct gcacagttta ggatgacggt acgggcgttt tctgtttcaa tccgccccat
    11521 ccgccaaaca taaaaatgcc gtctgaaacc gttgcaagtt tcagacggca tttcgcttat
    11581 ccgcttattt tttgctcaaa gtcagccccg tccaaccgaa taacagggtc aaagccaaac
    11641 tcaaatagca gaaaaaggca tacggcagat attcccaaac cggcacgccc agcgcgtggc
    11701 tgatgaacac gccgcatacg ctccacggta cgagcgggtt aatcaccgtc cccgcatctt
    11761 ccagcgtccg cgacagattg cgggaatgta gtccgagcct gtcgtaaacg ggtttaaacg
    11821 tttctccaga aagcaggatg ctcaaatatt gttcgccaat caggaaatta accccgaccg
    11881 aagtcatggc aacgctgaac gtcgcgcgtc cggcattcgt caagaaggta cggatggcct
    11941 ccaacaggga aggaatcaca ccgagcgcaa acaacagtcc gcccaaactc atcccgagaa
    12001 tcacgatggt ttgcgtgaaa aacatacttt ccaaaccgcc gcgcgaaatc agtttgacaa
    12061 catctttaaa cgcttcgcct tcgagtttgt agccgccgta aaaccacgca ccgagctgac
    12121 gcagatcggg cgtgctgtgc agatacgtta cagcaacggc aaccatgacg gtaaagagca
    12181 tggcgacgac ggcgttgatg cgcatcaatg ccaaaatgac caacagcgca aacggaatca
    12241 gcgaatagcc gtgcaccaat cccgtggctt caagctggct gcggaaggat tcgacgctgt
    12301 tcaaatcctg cgcggcgaca ttcggcaaaa gccaaagcat cagtgccgca ctaatgagcc
    12361 acgcggggat ggtggtgtac atcatatttt tgatgtgctc aaacaagtcg atgccgacga
    12421 tggacgcgga aatacccgtc gtatccgaaa gcggggacat tttgtcgcca aaaaatgcgc
    12481 ccgaaacaat cgcgcccgcc gtcatcgcca tatcggcctg aaacgccgcc gccatcccca
    12541 taaaggcaac gccgacagtg gcgcaggtgg tcaggctgct gccgatggac acgccgatga
    12601 cggaacacag cgcgaaggcg gaaaaataaa aataagtcgg agaaatcagc ccgaaaccgt
    12661 aatacatcag cgtcggaatc gcaccgctca tcatcagtgc gctgaccatc agcccgatga
    12721 agaaaaacag gtaaatcgcg cccataccct gattcaacgc gcctatcatg ccctgctgca
    12781 tatcgttgta tttcaaaccg cgcgccaagc cgtacaaaat cagcacgacg atggcggcaa
    12841 taatggacat atgcggcagc cactccaatg aaatgatggt atagcccatc gcggcaatca
    12901 gagcgacgac gacggcaagt gcctcaccgc gcggcatatc gagtaaggat ttgaaagcaa
    12961 acatgattct ttgcccgatt tgatataaag ttaacggatt ctaacacact ataccgaatc
    13021 cgcccgacgt tttcagacgg cattttagcg ttttacaaaa gccggacaga aacaaaacgg
    13081 caattttctc aatttaaatt aatttttttg aaatataatt ttttattttt ttgtttgttg
    13141 gcatataaga cttttatgga ttgaaatatt aaaacctcgg cacacacgcc gcaaacaccg
    13201 gtacgcaaaa atgccgtctg aaagatgttc agacgacatt tctcaagaaa aagattattt
    13261 gtccgcccta tatccgtaca ccgcaaaaaa cacgatatac agataacaga tgacggaaac
    13321 gacaaacgaa gacatcaggg tgtaagtatc tgccacccag ccctgcacga caggaacgac
    13381 cgcaccgccg acaatcgcgg tacacagtac accggaagcc gcgttggtaa attttcccaa
    13441 tcctttggtt gccaaagaga aaatcgtcgg gaacataatc gagttgaaaa aaccgatggc
    13501 aagcagcgac cacatcgcca catccgcatt gccgctaccc gtcgccatcg cgatggcaag
    13561 cagtacgacc gcagccgatg cgttaaacgc caaataacgg ttgggcgcga atttcgccat
    13621 caccgccgaa ccgaggaaac gtccgaccat cgcgccgccc caatagaacg acaggtaatg
    13681 cgcggcagaa gcatgatcca gccctttcag ataacccaat acgttgacca tcaacgaacc
    13741 gatagacacc tccgcgccga catagcagaa aatgcctgcc gtaccgaaca cgagatgttt
    13801 gtattgccat acgccggttt tgccgtcgtg gttgtgcgcg ctttcctcgg cggcaatttt
    13861 gcgcgcgtcg ggcagccgga tcattttcac gaaaacggca aggataatca gcagccccgc
    13921 cagtcccaaa tagggaatct gtacggaaga aatctgttcc gccttgctga cggtttgggt
    13981 tgcgtccgcc aaaatcagga acgcgccgat ttgcggcgca atggtcgtac ccaaagcgtt
    14041 aaacgcctga accagcgtca gtgttgccga ttccttgccg ggtttcgcca gcagggtaac
    14101 ataaggatta ccggcaacct gaagcagcgt tacgccggaa gccaaaataa acaacgcgcc
    14161 caaaaatacc gcgtaagaat ggctgcccgc agcaggataa aacagcaggc atccgaccgc
    14221 cgtcagcaga aagccgccga taacgccgtt tttgtagccg actttgccga caaaagcccc
    14281 catcgggatg gacatcaccg cataggcggt aaagaaacag aattggatca gcatcgcctg
    14341 aacgtaagac aggtcgaaaa tttctttcaa atgagggata aggatgtcgt tcatgcaggt
    14401 aataaaaccc atcatgaaga acagcgtggt caagacgacc aatggggacg tatgattgtt
    14461 ttgtgattgc gcagacatga tttgcctttc gtggtgtcgg aatattaccc gtttcaagaa
    14521 aatagaaatt gacatttctt aacggcaatg caaatcatac tgatatttga acattttgca
    14581 aactttatcg gatgcaggca atgcgccgat ctgaaattca agcagcagaa attaatattt
    14641 tacagttgct tgcatagatt ttaatagatt ttaaaaaatt ccggcggact tgcaaaaacc
    14701 gttcccaata aaatgaaaat ccgccgccgt atccggaaaa ttcggaaatg ccgcatcgtg
    14761 gcatcaaaac cggagcggaa agaatgtcgt atgcccgaca aaaaaggggc atacggctgt
    14821 gccgtatttt ttgcaccgtc ctccgccctt cggcgggggc gtgaaaatat agtggattaa
    14881 caaaaatcag gacaaggcga cg
//
LOCUS       DAWFAD010000042        14716 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWFAD010000042 DAWFAD010000000
VERSION     DAWFAD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14716
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.042"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(24..1067)
                     /gene="galE"
                     /locus_tag="WGO49_000932"
                     /pseudo
     CDS             complement(24..1067)
                     /gene="galE"
                     /locus_tag="WGO49_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223275.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            complement(1060..2058)
                     /gene="dcm"
                     /locus_tag="WGO49_000933"
     CDS             complement(1060..2058)
                     /gene="dcm"
                     /locus_tag="WGO49_000933"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0306055.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKPAVCITGDMRKVFHY
                     EQNRALTVRELAALQSFPDNFIFCGSKIAQQQVGNAVPPLLAKAIAESILKMSENE"
     gene            complement(2089..4362)
                     /locus_tag="WGO49_000934"
     CDS             complement(2089..4362)
                     /locus_tag="WGO49_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ0306056.1"
                     /translation="MNITQILSQELSATTAQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDGLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETESEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(4427..5077)
                     /locus_tag="WGO49_000935"
     CDS             complement(4427..5077)
                     /locus_tag="WGO49_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0306057.1"
                     /translation="MISVEHVSKQYQMRGGMRTVLDDINFSLQKGEKVGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMHQFEDMDKAYEYYNSLP"
     gene            complement(5074..5871)
                     /locus_tag="WGO49_000936"
     CDS             complement(5074..5871)
                     /locus_tag="WGO49_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0306058.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            complement(5871..7034)
                     /gene="ctrB"
                     /locus_tag="WGO49_000937"
     CDS             complement(5871..7034)
                     /gene="ctrB"
                     /locus_tag="WGO49_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ0306059.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(7049..8224)
                     /gene="ctrA"
                     /locus_tag="WGO49_000938"
     CDS             complement(7049..8224)
                     /gene="ctrA"
                     /locus_tag="WGO49_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ0306060.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            8359..9492
                     /gene="neuC"
                     /locus_tag="WGO49_000939"
     CDS             8359..9492
                     /gene="neuC"
                     /locus_tag="WGO49_000939"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ0306061.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALEVSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            9496..10182
                     /gene="neuA"
                     /locus_tag="WGO49_000940"
     CDS             9496..10182
                     /gene="neuA"
                     /locus_tag="WGO49_000940"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ0306062.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            10183..11232
                     /gene="siaC"
                     /locus_tag="WGO49_000941"
     CDS             10183..11232
                     /gene="siaC"
                     /locus_tag="WGO49_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ0306063.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIS"
     gene            11384..14497
                     /locus_tag="WGO49_000942"
     CDS             11384..14497
                     /locus_tag="WGO49_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0306064.1"
                     /translation="MAVIIFVNGIRAVNGLVKSSINTANAFAEEGLDVHLINFVGNIT
                     GAEHLYPPFHLHPNVKTSSIIDLFNDIPENVSCRNTPFYSIHQQFFKAEYSAHYKHVL
                     MKIESLLSAEDSIIFTHPLQLEMYRLANNDIKSKAKLIVQIHGNYMEEIHNYEILARN
                     IDYVDYLQTVSDEMLEEMHSHFKIKKDKLVFIPNITYPISLEKKEADFFIKDNEDIDN
                     AQKFKRISIVGSIQPRKNQLDAIKIINKIKNENYILQIYGKSINKDYFELIKKYIKDN
                     KLQNRILFKGESSEQEIYENTDILIMTSESEGFPYIFMEGMVYDIPIVVYDFKYGAND
                     YSNYNENGCVFKTGDISGMAKKIIELLNNPEKYKELVQYNHNRFLKEYAKDVVMAKYF
                     TILPRSFNNVSLSSAFSRKELDEFQNITFSIEDSNDLAHIWNFELTNPAQNMNFFALV
                     GKRKFPMDAHIQGTQCTIKIAHKKTGNLLSLLLKKRNQLNLSRGYTLIAEDNSYEKYI
                     GAISNKGNFEIIANKKSSLVTINKSTLELHEIPHELHQNKLLIALPNMQTPLKITDDN
                     LIPIQASIKLEKIGNTYYPCFLPSGIFNNICLDYGEESKIINFSKYSYKYIYDSIRHI
                     EQHTDISDIIVCNVYSWELIRASVIESLMEFTGKWEKHFQTSPKIDYRFDHEGKRSMD
                     DVFSEETFIMEFPRKNGIDKKTAAFQNIPNSIVMEYPQTNGYSMRSHSLKSNVVAAKH
                     FLEKLNKIKVDIKFKKHDLANIKKMNRIIYEHLGININIEAFLKPRLEKFKREEKYFH
                     DFFKRNNFKEVIFPSTYWNPGIICAAHKQGIKVSDIQYAAITPYHPAYFKSPKSHYVA
                     DKLFLWSEYWNHELLPNPTREIGSGAAYWYALDDVRFSEKLNYDYIFLSQSRISSRLL
                     SFAIEFALKNPQLQLLFSKHPDENIDLKNRIIPDNLIISTESSIQGINESRVAVGVYS
                     TSLFEALACGKQTFVVKYPGYEIMSNEIDSGLFFAVETPEEMLEKTSPNWVAVADIEN
                     QFFGQEK"
BASE COUNT         4369 a         3312 c         2921 g         4114 t
ORIGIN      
        1 gtttttttgt ttgcggtttg catttaatcc ccatatccgt tggggttgcg gctgacccaa
       61 cgccacgaat cttccatcat ttgctgcaag ccgcgtttgg tttcccagcc ggtttgttgt
      121 ttggtatggg acgggtcggc aaaggaacac gccaaatcac cggcgcggcg cggtttgatt
      181 tcaaatggaa tggttaaacc cgaagccgct tcaaatgcgc ggatgatttc caataccgaa
      241 gaagcgcggc cggagcctaa gttcagcaaa tgcgtgcctg ctacattact ttttgcctgc
      301 atagccgcga catggccttc tgccaaatcc atcacatgaa tatagtcacg catccccgtg
      361 ccgtcggggg tagggtagtc atcgccaaat accgccaatt gcggcagttt gcctgccgcc
      421 acttggcaga tataaggcaa caaattattc gggatgccgt ttggctgctc gccaatcaaa
      481 ccactttcgt gcgccccaat cgggttgaaa tagcgcaaca aaatcacgct ccaacgcgga
      541 tcggcctttt ggatgtcggt taacatccgt tccaccatcg ctttggatgc gccataagga
      601 ttagcggtat cgcccgggcg catatcttcc gtatagggca ttttacgtat catggcgcga
      661 cgcattaaag taaacgccaa agcgcgttta tactggttat ccagtttccc tatattttta
      721 cgttacaaat caagttgttg gtattcatcg tgaaaataga atttttcggc ataacgctga
      781 ctcataaaac cggcatgcgg gctacctgaa aaaatcattg cgccatcatc gtcatttaat
      841 gtttcatggt tgttttctat taatgctaaa gcaatttggt aattaattgc caaaatatcg
      901 ttagtaatca cacgatagcc gcgttttttg gcttcatagg caaaggaaca gcctccacta
      961 aatacatctg caactgtatc tacatcagac ggaagctggt cacaaatcca ggaagctatt
     1021 ttctctttat taccgatata gttaattttc ggatattgct tattcatttt cactcatttt
     1081 taaaatactt tcagcaatag cttttgccaa taaaggcggt acggcgttac caacctgctg
     1141 ctgggcaatt ttgctgccgc aaaaaataaa attatcaggg aaagattgta aggcagctaa
     1201 ttcacgaacg gttaaagccc gattctgttc atagtgaaaa actttgcgca tatctcctgt
     1261 aatacaaacg gctggtttgt tgctgttgta acggatgtat ttacggatat cacctgtttt
     1321 cggacgtaat ggttcaggaa tatcgttacg gttacctcca tttttaacaa atgccatttt
     1381 ttctaacatt tgtgccgaat gattcatagc ttcatgattt gcaacgtgtg gattgctttc
     1441 gccagcagcc agttttggaa aatgtcctat tgctgatcca acagtctgat gggaaatctg
     1501 caaaggttcg ggaaaggaaa ttttgccttt atccctcctc ccgataaata tcactcggct
     1561 acgtatctga ggaacaccga aatcggctgc actcagtatc ttacattcca ccgaataacc
     1621 gatattctga aatgcttgaa taatctcaat acgtgtttta cctgaattgt gtgtatagag
     1681 tcgcgctaca ttttccataa caaaaaaata tggttggaca attttaacta ttcggacaaa
     1741 ctctttaaat aaatggttgc gtgggtcatc tgtaaatgtc cgtccaatct ttcctgccat
     1801 actaaaacct tgacaaggtg gtcctccaat aatcaaatca actgcttgtc cgttaagaca
     1861 attgattaaa tcttgttcgg ttagtgtggt taaatctttt tgcagtaatt gatgatgggg
     1921 gaagttggta cggtaagtct gacaataatc agactccatt tcaacagaaa gcaattgttg
     1981 gaatccggct tgttcaaaac ccaaggatag gcctcctgct cctgagaaaa ggtcaatata
     2041 ggtaagtggt gtgtgcataa gtcaaaatcc ataaactctt cgattatttt accgcttcag
     2101 cttcgcaaac gcatccgcca tcgccgaatt ggctggggcg cggtcgttgc gttggggttt
     2161 gcggccggct gtccgttcgc ggctgcggtt ttcagacggc attttatgtt ttgcgccgcc
     2221 cggttcgtca tccaagcgca tggtcagcgc gatgcgtttg cgtgcagcat cgacttccag
     2281 cactttcact ttcaccacgt cgccagcttt caccacttcg cgcgggtctt ggacgaactt
     2341 gttggacagg gcggagatgt gcaccaagcc gtcctgatgg acgccgatgt ccacgaacgc
     2401 gccgaagttg gcgacgttgg aaaccacgcc ttcgagtatc ataccgactt gcaagtcgct
     2461 gatttcgtgg ataccttcgg caaacgatgc cgtctgaaac tcgccgcgcg gatcacggcc
     2521 gggtttttcc agttcggaca ggatgtccaa aatggtcggc aggccgaagc gttcgtcggt
     2581 gaagtcggac gctttgattt gcttcacgcg ctcgcggttg ccgatgagtt cggcggcgct
     2641 aatgccttgt tgcgccagca ttttggcgac gacgggatag gcttcggggt ggacggcgct
     2701 cgcgtccaac ggctctttac cgccgttaat ccgcaaaaag cctgccgcct gctcgaaggt
     2761 tttttcgccc aaacgcggta ctttcagcaa ttttttgcgg ctgtcgaacg cgccgttttc
     2821 atcgcggtag gcaacgatgt tttgggcaag ggtttgattc aaaccggaaa ttcgcgccaa
     2881 gagcggggcg gaggcggtgt tcacgtccac gccgacggcg ttcacgcagt cttcgaccac
     2941 cgcgtccagc gatttggcga gctggctttg gttcacgtcg tgctgatact gccccacgcc
     3001 gatggatttg gggtcgattt tgaccaactc ggcaagcggg tcttgtagcc tgcgggcgat
     3061 ggacaccgcg ccgcgcaggg aaacgtccaa gtcggggaac tcgcgcgccg ccagttcgga
     3121 cgcggaataa atcgacgcgc cggcttcgga cacgacgatt ttatgcagcc ccattttcga
     3181 catttcacgc accagttcgc ccgcgatttt gtcggtttca cggctggcgg tgccgttacc
     3241 gatggcgatg agcttcacgc cgtgctgttt aatcaggcgc gacagcgttg ccaacatatt
     3301 gttttcttga tgcaaataga cgatgacggt atccagcagc ttgccggtgt cgtccaccac
     3361 ggcgcatttc acgccgttgc ggtagccggg gtcgagaccc agcgtggtca gccgtccggc
     3421 gggcgcagca agcagcaagt ctttgagatt gcgggcgaac acggtaatcg cgtcggtgtc
     3481 ggcggcttct ttcaaacgat tgagagcttc aagttccaac gacaaaaaga ttttcgcgcg
     3541 ccaagtcagg cgcacggtgt cgcgcagcca tttgtggccg tctgaaactt tgaagcggcg
     3601 ggcgatgatt tgctcgtatt cgctttgctg cgtaatcggc gtgtcgtcgg gctgatattt
     3661 gagcgcgatg ttcaacacgc cttcgttgcg tccgcgcaaa accgctagcg cacgatggct
     3721 gggcatcgca cgaataggtt cgcggtggtc aaaataatcg ctgaattttt cgctttcggt
     3781 ttctttgcct ccaacaactt gcgcgtggat ttcggcttcg ttccacagct tgtcgcgcag
     3841 cgtgccgatg agttccgcgt cttcggcaaa ctgctccatc agaatcgcac gcgcgccgtc
     3901 caacgcggct ttggcatcgg ggacgttttc gttcaggtag ccttgtgcgg cggcttccac
     3961 gtcctgcgac tgctcggcaa gcaacacgtc cgccagcggc tgcaaaccgt gttcgcgcgc
     4021 aatttgcgcc ttggtgcggc gtttgggctt gtagggcaga tacaggtctt ccagcgcggt
     4081 tttgttgtcg gcggcttcga tttgcgcgcg gaggccgtct gaaagtttgc cttgctcttc
     4141 aatgcttttt aacacaacgg ctttgcgctc ttccaactcg cgcaggtatt gcagccgctc
     4201 ggcaagccgg cgcagctgcg tgtcgtccag cccgccggtg gcttctttac ggtaacgcgc
     4261 gataaacggc acggttgcgc cgtcgtctaa aagctcaata gcggcattga tttgcgcggt
     4321 agtcgcggaa agttcttggg aaagaatttg agtaatgttc atcaatagaa ttccaacgga
     4381 caggccgtct gaaatttcag acggcctgat ttaaaaaaca atcgctttaa ggcagcgaat
     4441 tataatattc gtaggctttg tccatatctt caaactggtg catatgccct ttttccagca
     4501 ccatcgcatt atcgcaatat tgcttcatgg cgctgtggct gtgcgacacc aagatgatgg
     4561 aacggtcttt gcgcttttca aacaactcgt acttacattt atcggcaaag cgcgagtcac
     4621 caactgcaat cacttcgtca atcaaatagc aatcaaactc taccgccaac gacaaagcaa
     4681 atgccaaacg cgccttcata cctgaagaat aacgcttgac cggctcgtaa agatattgcc
     4741 ccaactcgga aaactcttcg gtaaagttct tcacataatc gatatcgaca ttgtaaatcc
     4801 ggcagatgaa acgcaaattg tccataccgg tcagactacc ttgaaacgca ccggagaatg
     4861 ccaaaggcca agaaatactc attgtccgct tgatttcacc cgtggtcggc ggctcgacac
     4921 cactaatcaa acggatcaac gtcgatttac ctgcaccgtt tcgacccaaa ataccaactt
     4981 tctcaccctt ttgcagcgaa aaattgatat cgtctaacac cgtccgcata ccgccgcgca
     5041 tttgatactg cttggaaaca tgttcaaccg aaatcattgc ggctcgactc ctttactgaa
     5101 tttactgacc atcgccaagc caaacaacaa caacaccaga ttgcacaata cgatatacca
     5161 aggattttca taggtaatta catcgctgcc aaaatatccg gcacggaaca tttctgtgcc
     5221 atgcaccatc ggaatcatta atgcatattc ttgtaccttg ggcggcaaat tatgcacaaa
     5281 aaagaacgca ccggataacg gcatcatcac aaaagtcaat gtgccccaaa tcttgccaaa
     5341 cggctcgaaa ttaaaggcaa tcgaacaaat caccaaaccc aaaccaatcg caaaaaaagc
     5401 catcaaaagc caagccatca gcatataaaa catatctgcc ggcatttcaa tccagccaat
     5461 cgcaatcaat accgccataa tcacaatctg cgcaatggtt gcaccagcaa tttccaaaat
     5521 catgcgcgcc aagatggtat ccaaaactct tacattgcgg tgataaagca agctggcatt
     5581 tgaagaaatc gacccaactg cccgttttga ggcattacgc cacatcatca acatcggata
     5641 gccagtaatc gcaaatgcga caatattcaa agttgaatat cggtctgccc ttaaaaattt
     5701 ccacatcaag acgataacga atgtcatcag caacggctca acaaacagcc ataaaaagcc
     5761 aatattattg cgaccgtaac gggtgataat ttcccgcatc aacagcgcac cgattacgcg
     5821 cctttgaatg gctaaagatt cccaaaatga tgttttatgc aaggctttca tcagttttta
     5881 tgctcacgaa tgctggcagt caacaggctc aaaataccat aaaccatcaa gccgataatc
     5941 agagtggcaa caatgttgta taaccgttta ggctcatgtg ccaaatccgg caggctcggt
     6001 tgcgagatca cttccaaata aagctgctga cggtctgctt caaccttggc actttccaaa
     6061 gaagtcatgg cggctgccaa ctgctgctct gccaactggt tttccaaata cacacgctga
     6121 tattcggcag cctgattaga caacgaagaa tgcccaccgc ccgaaatggc acgtaactgt
     6181 tggtcaattt ctttacgcaa gctctgctca cgcgcctgca aacccggaat ctgcggattc
     6241 tccggagtga ctgctttcac ctgatccagc tgggtttgaa tcacaatcaa ttcatcttgc
     6301 agcttggaaa ccaaccccat ttgcacttcc gattgcgctt tcaaatcaaa aacgccattg
     6361 gcaatccggt aatccgtcag attctgagag gcttccttta cccgctctgc cgccgttttc
     6421 actacttctt ccgcatagcg caccgtatca gcacgtgcac gatcgttcaa ctggttaatc
     6481 aatgcttcac cttgttttaa caaagcctca ttgattttct tagattccag cgcatcaaag
     6541 gaagttacat tcaacgtgga aatacccgaa accgtatcaa aattgatcat cacctgattt
     6601 ttatagtatt gataaaaagc ctcttcctcg ccacggaacc caaacccatt aaagcggctg
     6661 aacgcatcac ctttggtttc ataaaactca cgcaccggca agattttacg cagttcatcc
     6721 aaagacgagc gcgaacgcat atactcccca accgtgtaaa tatcatcttg cgcacgggca
     6781 aaacctgtgc cctgcaaaat ggcacccagg ccattgagag aagattggct tttaggcgag
     6841 cgcaccacaa agctcgattg cgacgtaaaa cgatcggaag cgaagaagcc gaaatacacc
     6901 aacgaaatta ccgtagggat aatcaccgtt acccaaaata aagggcttag ctttttaatc
     6961 caacttttct ttttcggctt tttacgctcg gctttggttt cagtggcaac tgccacagga
     7021 agttgctcag acatcttact tactcacatt aattagttaa attattaata ctgttcgcgc
     7081 cactggtaac cggcgagaac acaaacgaca aaaatttctg cacttcagcc aacggcgcat
     7141 tcgacacata caatacatct ttattcttca caggaaagcg ctgcatagaa aatagcgaat
     7201 gcgcatcagc catattcaca cgatataccg ttggaatctc tgcctcactg ccataacctt
     7261 gagcaatcca tttatcctga cgttctgccg gcaattccac caatggcgta tagcggaaca
     7321 caaacacacc acgcgcatca gaacggcgat cttgcaaacc gcccatacgg ccaatggctt
     7381 cagaaagcga taagcctctg gctgaaaaac cgatttcttg tgttctcccc accgcaccca
     7441 tagacgtaaa ggtataggga ttggtaatca tggtaaccac atcaccgcga cgcagcaaaa
     7501 tattttgtcg cggatttgca actaaatctt ccaaggcaac agttcgtact acattgccac
     7561 gtgtcagctg cacattcgta tcctgcacat ttgccgttga accacctacc gcagccaccg
     7621 catccaacac acgctcaccg gctgccgtca gcggcatacg cacactattc ccagcacgaa
     7681 tcaccgacac attcgccgca ttattctgca ccaaacgcac catcacttgt ggctgattgg
     7741 ccattttttt caggcggcct ttaataattt cctgaacctg accaggcgtt ttaccgacca
     7801 ccgaaatatc gccaacaaac ggcacagaaa ccgtaccacg tgccgtgacc aactgctctg
     7861 gcaacttagt ttgatgcgca ctacccgagc ccatcgaaga aaggccacca ccaaacaata
     7921 ctgccggcgg cgcttcccaa atcataatat ccaatacatc accaatattt agcgtaccag
     7981 ccgaagcata accatcgcca aactgagtga atgactgatt tatctgagcc ttatataata
     8041 actgagcaac cgtatgattc acatcaatca gctccacttc aggaatttga acttcagatt
     8101 gttgccctaa agagacaatt ttttttgcgc tggggcctga tgaaggaatc gcagagcatc
     8161 ctacaattaa acttccacac aataataata ctgcgtgacg aatataaaat ttcactttaa
     8221 acacaagcca aatcctaata taattataaa tggcctaatt atagcactta atcgaaataa
     8281 atttatgagt acgtagagta taattagtat tcttctttcc aacttcctta tacttatata
     8341 cttatagatt ctaaaatcat gaaaagaatt ctttgcatta caggtaccag agccgacttc
     8401 ggcaagctaa aacctttatt agcctatatt gaaaatcacc cagaccttga attgcatttg
     8461 attgtaactg gtatgcatat gatgaaaaca tatggcagaa cctgcaagga agtaactcga
     8521 gaaaactatc aacatacata tctgttttca aatcaaatcc aaggtgaacc aatgggtgcc
     8581 gttttaggca ataccattac gtttatctct cgtctatctg atgaaattga acctgatatg
     8641 gtcatgattc acggcgaccg tttagaagca ctagcaggcg caactgtagg tgcattaagc
     8701 agccgtttag tttgccatat cgaaggtggt gaactatctg gtacagtaga tgactccatt
     8761 cgtcattcta ttagtaaact ttctcatatc cacttggtag caaatgaaca agctgtcact
     8821 cgcctagtgc aaatgggaga aaaaagaaag catattcaca tcatcggctc ccccgattta
     8881 gatgttatgg cctcttccac cctcccatcc ttagaagaag tcaaagaata ttacggttta
     8941 ccatacgaaa attatggtat ttctatgttt caccccgtga ctacagaagc acatttaatg
     9001 ccacaatatg cggcccaata tttcaaagca ttagaagtaa gtggccaaaa tatcattagc
     9061 atctacccta ataatgacac tggcactgaa agtattctgc aagaattatt aaaatatcaa
     9121 tctgataaat ttattgcatt tccttccatt cgatttgaat actttttagt cttattgaaa
     9181 catgctaaat ttatggtcgg caactcaagt gcaggtatta gagaagctcc tctctacggt
     9241 gtcccttcaa ttgatgttgg tacacgccaa agtaaccgcc atatgggaaa atctattatt
     9301 catacagatt atgaaactaa aaatatcttt gatgcgattc aacaagcatg cagtttaggc
     9361 aaatttgaag cagatgatac ctttaatggc ggagatactc gcaccagcac agaaagattt
     9421 gctgaagtaa tcaacaatcc tgaaacgtgg aatgtttctg ctcaaaaacg ttttatcgat
     9481 ttgaatcttt aaattatgga aaaacaaaat attgcggtta tacttgcgcg ccaaaactcc
     9541 aaaggattgc cattaaaaaa tctccggaaa atgaatggca tatcattact tggtcataca
     9601 attaatgctg ctatatcatc aaagtgtttt gaccgcataa ttgtttcgac tgatggcggg
     9661 ttaattgcag aagaagctaa aaatttcggt gtcgaagtcg tcctacgccc tgcagagctg
     9721 gcctccgata cagccagctc tatttcaggt gtaatacatg ctttagaaac aattggcagt
     9781 aattccggca cagtaaccct attacaacca accagtccat tacgcacagg ggctcatatt
     9841 cgtgaagctt tttctctatt tgatgagaaa ataaaaggat ccgttgtctc tgcatgccca
     9901 atggagcatc atccactaaa aaccctgctt caaatcaata atggcgaata tgcccccatg
     9961 cgccatctaa gcgatttgga gcagcctcgc caacaattac ctcaggcatt taggcctaat
    10021 ggtgcaattt acattaatga tactgcttca ctaattgcaa ataattgttt ttttatcgcc
    10081 ccaaccaaac tttatattat gtctcatcaa gactctatcg atattgatac tgagcttgat
    10141 ttacaacagg cagaaaacat tcttaatcac aaggaaagct aaatgcaaaa caacaacgaa
    10201 tttaaaattg gtaatcgttc agtaggttac aaccacgaac cattgattat ctgtgaaatc
    10261 ggcatcaatc atgaaggctc tttaaaaaca gcttttgaaa tggttgatgc tgcctataat
    10321 gcaggcgctg aagttgttaa acatcaaaca cacatcgttg aagacgaaat gtctgatgag
    10381 gccaaacaag tcattccagg caatgcagat gtctctattt atgaaattat ggaacgttgc
    10441 gccctgaatg aagaagatga gattaaatta aaagaatacg tagagagtaa gggtatgatt
    10501 tttatcagta ctcctttctc tcgtgcagct gctttacgat tacaacgtat ggatattcca
    10561 gcatataaaa tcggctctgg cgaatgtaat aactacccat taattaaact ggtggcctct
    10621 tttggtaagc ctattattct ctctaccggc atgaattcta ttgaaagcat caaaaagtcg
    10681 gtagaaatta ttcgagaagc aggggtacct tatgctttgc ttcactgtac caacatctac
    10741 ccaacccctt acgaagatgt tcgattgggt ggtatgaacg atttatctga agcctttcca
    10801 gacgcaatca ttggcctgtc tgaccatacc ttagataact atgcttgctt aggagcagta
    10861 gctttaggcg gttcgatttt agagcgtcac tttactgacc gcatggatcg cccaggtccg
    10921 gatattgtat gctctatgaa tccggatact tttaaagagc tcaagcaagg cgctcatgct
    10981 ttaaaattgg cacgcggcgg caaaaaagac acgattatcg cgggagaaaa gccaactaaa
    11041 gatttcgcct ttgcatctgt cgtagcagat aaagacatta aaaaaggaga actgttgtcc
    11101 ggagataacc tatgggttaa acgcccaggc aatggagact tcagcgtcaa cgaatatgaa
    11161 acattatttg gtaaggtcgc tgcttgcaat attcgcaaag gtgctcaaat caaaaaaact
    11221 gatattagtt aataataaaa tagattaagc tattcttaaa ttcagaatat tgcttatcta
    11281 tattaaaaat ttctaatttt taaggttctg attgaaatca gaaccttatt tcaactatta
    11341 ctttttactc ataatcgaat tatatacttt aggactttat aatatggctg ttattatatt
    11401 tgttaacgga attcgggctg taaatggcct tgttaaatca tctatcaata ctgcaaacgc
    11461 ttttgctgaa gaaggactgg atgttcattt aattaatttt gttggcaata ttactggagc
    11521 agagcattta taccccccat tccacttaca tcccaatgtc aaaacctcca gcatcataga
    11581 tttatttaat gacattccag aaaatgttag ctgccgaaat actccttttt attctattca
    11641 tcaacaattc ttcaaagctg aatatagtgc ccactataag catgttttga tgaaaattga
    11701 atctttatta tctgcagaag atagcattat cttcactcat cctcttcaac tggaaatgta
    11761 tcgtttagcg aataatgata tcaagtcaaa agccaaacta attgtacaaa ttcatggtaa
    11821 ttatatggaa gaaatccata actatgaaat tttggcacga aatatcgatt atgttgacta
    11881 tcttcaaacg gtatctgatg aaatgctgga agaaatgcat tcccatttca aaatcaaaaa
    11941 agacaaatta gtttttattc caaacatcac ttatcccatt tcattagaaa aaaaagaagc
    12001 tgatttcttt attaaggata atgaagacat cgataatgct cagaaattta aacgtatctc
    12061 tattgttggc agcattcagc caagaaaaaa ccaattggat gccattaaaa tcatcaataa
    12121 aattaaaaat gaaaattaca ttttacagat atatggcaaa tctattaata aagattactt
    12181 tgaattaatt aaaaaatata ttaaagacaa taagttacaa aaccgtatct tattcaaagg
    12241 tgaatcttcc gagcaggaaa tttatgaaaa tacagatatc ctgatcatga catcagaaag
    12301 tgagggattt ccatatatat ttatggaagg catggtgtat gatattccaa tcgttgtata
    12361 tgattttaaa tatggagcga atgattacag taactataat gaaaatggtt gtgtttttaa
    12421 aactggtgat atttctggaa tggcaaaaaa aataattgag ctattaaata acccagaaaa
    12481 atataaagaa ttagttcaat ataatcacaa tcgcttctta aaagaatatg caaaagatgt
    12541 ggttatggct aaatatttca ctattcttcc gcgcagcttt aataacgtat cattatcgtc
    12601 tgctttcagc cgaaaagaat tggacgaatt ccaaaatatt actttttcta ttgaagattc
    12661 taatgattta gctcatattt ggaatttcga gctaaccaat cctgcacaaa atatgaattt
    12721 ttttgcttta gttggcaagc gaaaatttcc aatggatgct catatccaag gaacacagtg
    12781 tacgattaag atagctcata aaaagacagg gaatttattg tcgcttttac taaaaaaacg
    12841 aaatcagttg aatttatcaa ggggatatac cttaattgca gaagataata gctatgaaaa
    12901 atatattgga gcaatatcta ataaaggtaa ctttgaaatt attgcaaata aaaagagctc
    12961 attagttact ataaacaaaa gtaccttaga gttgcatgag attccccatg aactacatca
    13021 gaataaatta ctgattgctt tacccaacat gcaaacgcct ctaaaaatta ctgatgataa
    13081 tttaatacct atccaagcct ccataaaatt agaaaagatt ggaaatactt attacccatg
    13141 tttcttgcca tctggcatat ttaataatat ctgcttagat tacggtgaag aatccaaaat
    13201 tattaatttt agtaaatatt cttataaata tatctatgac tcaattcgtc atattgagca
    13261 acatacagat atatcggata ttatcgtttg caatgtttat tcttgggaac ttattcgtgc
    13321 ctcagttatt gagagcctta tggaatttac cggaaaatgg gaaaaacact ttcagacttc
    13381 tcctaaaatt gattatcgat ttgatcatga aggtaagcgt tcgatggatg atgtcttttc
    13441 agaagaaaca tttattatgg aatttccgcg taaaaatggt atagataaga aaacagcagc
    13501 cttccaaaat ataccaaaca gtattgtaat ggagtatccg cagaccaatg gttacagtat
    13561 gcgcagtcat tcactgaaaa gtaatgtagt tgcggcaaaa cattttcttg aaaaattaaa
    13621 taaaattaag gtagatatta aatttaaaaa gcatgacctt gcaaacatca aaaaaatgaa
    13681 tcgaattatt tatgagcatt taggcattaa cataaatatc gaagcatttc taaaaccacg
    13741 attagaaaaa tttaagcgtg aagaaaaata ttttcatgat ttcttcaaaa gaaataattt
    13801 taaagaggta atttttccaa gcacttattg gaatccaggt attatttgtg ctgcacataa
    13861 acaaggtatt aaggtatctg atattcaata tgctgccatt actccttatc atcctgcgta
    13921 ttttaaatca ccaaaatcac attacgttgc tgataaattg ttcttatggt ctgaatattg
    13981 gaatcatgag cttttaccaa atccaacacg agagattggt tctggtgccg catattggta
    14041 tgcattagat gatgtgagat tttcagaaaa actgaattat gactatatct ttctatctca
    14101 aagtaggatt tcttcgcgct tgcttagttt tgcaattgag tttgcattaa aaaatcctca
    14161 actacagctt ttattttcta agcatccaga tgaaaatata gatttaaaga acagaattat
    14221 tcctgataat cttataatct ccacggaatc ttctatacaa ggcatcaatg aatctcgcgt
    14281 tgctgtaggt gtttattcaa ctagcttatt tgaggcatta gcatgcggca aacaaacttt
    14341 tgttgttaaa tatccgggat atgaaattat gtcaaatgaa atagattcag ggttattctt
    14401 tgcagtagaa acacctgaag aaatgcttga gaaaacaagc ccgaattggg tggctgtggc
    14461 agatattgaa aaccagtttt ttggccaaga aaaataattt ttaagttaat ctttaaaagt
    14521 gcaatatttt tcttatttta aatattgcac tttattcttt taatcatagg aactcacatg
    14581 tattctgaac agggaattaa taatactatt aacatatcta ctacatcttt aaccaatgcc
    14641 acgcaactaa ctgtgatcgg gaataataac tctgtatata taggtaataa ttgtaaaatc
    14701 gtcagctcca atatta
//
LOCUS       DAWFAD010000043        13791 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWFAD010000043 DAWFAD010000000
VERSION     DAWFAD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.043"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(129..1394)
                     /gene="ftsY"
                     /locus_tag="WGO49_000943"
     CDS             complement(129..1394)
                     /gene="ftsY"
                     /locus_tag="WGO49_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ0306065.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEEVE
                     SLTESVKEQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAIAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
     gene            1539..3107
                     /gene="msrAB"
                     /locus_tag="WGO49_000944"
     CDS             1539..3107
                     /gene="msrAB"
                     /locus_tag="WGO49_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ0306066.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            complement(3209..3694)
                     /locus_tag="WGO49_000945"
     CDS             complement(3209..3694)
                     /locus_tag="WGO49_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ0306067.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGMLAAAFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(3721..4569)
                     /locus_tag="WGO49_000946"
     CDS             complement(3721..4569)
                     /locus_tag="WGO49_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0306068.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            complement(4663..5664)
                     /locus_tag="WGO49_000947"
     CDS             complement(4663..5664)
                     /locus_tag="WGO49_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0306069.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            5723..6700
                     /locus_tag="WGO49_000948"
     CDS             5723..6700
                     /locus_tag="WGO49_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ0306070.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDAILTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVNALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKSHALPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTPYPPKIRKNVMRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTP
                     VYYQDGSYSKNMNYNQYRPERHAVLPDQTDGSEQDVRGQNWQKPKFQNR"
     gene            6755..8125
                     /gene="glmU"
                     /locus_tag="WGO49_000949"
     CDS             6755..8125
                     /gene="glmU"
                     /locus_tag="WGO49_000949"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ0306071.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVIGHGKDQVLNAVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDVPLIDVKTLKTLLEAAGNEVGLLTDVPADPTGLGRIIRDGNGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVHT
                     SYLAAGVNNKLQLAELERIFQTEQAQELLKAGVTLSDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVEIGDNVEIGANCVIKNAKIGANSKIAPFSHLEDCEVGENNRIGPYARLRPQ
                     AKLAADVHIGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGNEVRIGSNCVLVAPVTLGNKVTTGAGSAITRNVEDGKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            8202..8531
                     /locus_tag="WGO49_000950"
     CDS             8202..8531
                     /locus_tag="WGO49_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ0306072.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAALQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(8603..9868)
                     /gene="efeB"
                     /locus_tag="WGO49_000951"
     CDS             complement(8603..9868)
                     /gene="efeB"
                     /locus_tag="WGO49_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ0306073.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQLDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(10132..11298)
                     /locus_tag="WGO49_000952"
     CDS             complement(10132..11298)
                     /locus_tag="WGO49_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ0306074.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     VSELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            complement(11295..12134)
                     /locus_tag="WGO49_000953"
     CDS             complement(11295..12134)
                     /locus_tag="WGO49_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ0306075.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMVLWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            12345..13670
                     /locus_tag="WGO49_000954"
     CDS             12345..13670
                     /locus_tag="WGO49_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ0306076.1"
                     /translation="MKKYLFRAALYGISAAILAACQSKSIQTFPQPDTSVINGPDRPA
                     GIPDPAGTTVAGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNTGGTHTADLSQFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSGNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
BASE COUNT         3161 a         4131 c         3535 g         2964 t
ORIGIN      
        1 cgaaagtggg aatccagaat ctctaaagct tcaactaacc tttgaatatt gccgttatcc
       61 caaggtctag attcccgcct gcgcgggaat gacggcatcg gtctgctgtt ttcggacggc
      121 atttcggctc aatccagcag tgcgtccaca aacgcgcgcg cgtcaaacgg gcgcaaatcg
      181 tctatgcctt cgcccacgcc gatgtagcgg acggggacgg ggcggtcgga agcaagcgcg
      241 gcgaggatgc cgccttttgc cgtgccgtcg agtttggtca cgattaaacc ggtcagccct
      301 aatgcgtcgt caaaggcttt gacttggttg acggcgtttt gcccgatgtt ggcatcaagc
      361 acgacgatga tttcgtgcgg cgcgtcgggc atggcttttt gcagcacgcg tttcactttt
      421 ttgatttctt ccatcaaatg aagctgcgtg ggcaggcggc cggcggtgtc ggcaagcacg
      481 atgtcgatgc cgcgcgcttt ggcggcttgg acggcatcga agcacacggc ggcggaatcg
      541 cccgtggttt gcgaaatcac ggtaacgttg ttgcgttcgc cccatgcctg aagctgctca
      601 cgggcggcgg cgcggaaagt gtcgcctgcc gccagcaata cggatttgcc ctgcgcttgg
      661 aaatatttgg cgagtttgcc gatagacgtg gttttgcccg cgccgttgat gccggcaagc
      721 atgatgacaa acggctcttt ggtttcgggc aagaccaatg gtttttccag aggcttaatc
      781 aggtcgtaca aggcttcttt caacgcgccg cgcaattcgt tgccgtcttt cagccctttg
      841 aggctgacgc ggtcgcgcac gtctttcatc aggtattcgg tggcttccat gcccatatcg
      901 ctggtaatca gcacggtttc cagctcttcg tacaaatcct cgtcgatttg tccgccgccg
      961 aacacgcccg ccagcgattt cgccattttg tcgcgcgatt tggtcaaacc ttgtttcaaa
     1021 cgcgccgccc aaccgagctt gtgttcttca gttgttgcaa cggcttcttg aacttgcccg
     1081 acagcctcgc cgacggtttc ggcaatggct tcttttgccg cttcgacttg ttccgctgct
     1141 ttttccgccg cttcctctgc ttcagacagc atctcggcaa cggtttcctt tacttgttca
     1201 accgcaccgc tgacggtttc aacagccgat tcgacctgct ctttgacgct ttccgttaaa
     1261 gattcgactt cttctttaat attttcaatt acttgatcgg tttcggactc tgcttttgct
     1321 gcggtttcct gaatttgagc ctcctcgaga gccggcgttt cctgtttttt cttgcgacgg
     1381 aagaagctga acattgaatt ttccttttaa ttttagaaac ttgaaacagg gcgtattgta
     1441 gcgtatttta cgcggcaagg ttgtctgaaa atccgagctg taaggtttcg gcatctcaaa
     1501 cgtctaatca tgcaaaccgt ccacacagga aactcaaaat gaaacaccgt actttctttt
     1561 ccctttgcgc caagttcggc tgcctgcttg cgctgggtgc ttgttcgccc aaaatcgtcg
     1621 atgccggagc cgcgaccgtg ccgcacactt tatccacttt gaaaactgcg gacaaccgcc
     1681 ccgccagtgt ttacttgaaa aaagacaaac cgacgctgat taaattttgg gcgagctggt
     1741 gtcctttgtg tttgtccgaa ttgggacaga ccgaaaaatg ggcgcaagat gcaaaattca
     1801 gctccgccaa cctgattacc gtcgcgtctc cgggcttttt gcacgagaaa aaagacggcg
     1861 acttccaaaa atggtatgcc ggtttgaatt atcccaagct gcccgtcgta accgacaacg
     1921 gcggcacgat cgcccaaagc ctgaatatca gcgtttaccc ctcgtgggcg ttaatcggta
     1981 aagacggcga cgtgcagcgc atcgtcaaag gcagcatcaa cgaagcgcag gcgttggcgt
     2041 taatccgcga cccgaatgcc gatttgggca gcttgaaaca ttcgttctac aaacccgaca
     2101 ctcagaaaaa ggattcaaaa atcatgaaca cacgcaccat ttacctcgcc ggcggctgct
     2161 tctggggctt ggaagcctat ttccaacgca tcgacggcgt ggttgacgcg gtatccggct
     2221 acgccaacgg caacacgaaa aatccgagct atgaagacgt gtcctaccgc catacgggcc
     2281 acgccgaaac cgttaaagtg acctacgatg ccgacaaact cagcctagac gacatcctgc
     2341 aatatttctt ccgcgtcgtt gatccgacca gcctcaacaa acagggcaac gacaccggta
     2401 cgcaataccg cagcggcgtg tactacaccg accccgccga aaaagccgtc atcgccgccg
     2461 ccctcaaacg cgagcagcaa aaataccaac tgcccctcgt tgttgaaaac gagccgctga
     2521 aaaacttcta cgatgccgag gaataccatc aggactacct gattaaaaac cccaacggct
     2581 actgccacat cgatatccgc aaagccgacg aaccgctgcc gggcaaaacc aagaccgccc
     2641 cgcaaggcaa aggcttcgac gcggcaacgt ataaaaaacc gagtgacgcc gaactcaaac
     2701 gcaccctgac cgaagagcaa taccaagtta cccaaaacag cgcgaccgaa tatgccttca
     2761 gccacgaata cgaccatttg ttcaaacccg gcatttatgt ggacgttgtc agcggcgaac
     2821 ctttgttcag ctccgccgac aaatatgatt ccggctgcgg ctggccgagc ttcacgcgcc
     2881 cgattgatgc aaaatccgtt accgaacacg atgatttcag ctacaacatg cgccgcaccg
     2941 aagtgcgcag ccacgccgcc gattcgcatt tgggacacgt cttccccgac ggcccgcgcg
     3001 acaaaggcgg actgcgctac tgcatcaacg gcgcgagctt gaaattcatc ccgctggaac
     3061 aaatggacgc ggcaggctac ggcgcgttga agagtaaagt gaaataagcc gcaccgccgc
     3121 ctaacccgac aaaataccgt ctgaaacctg caacgtttca gacggtattt tttatccggc
     3181 ggggattctg tccagacgga gattttgttt agacagcatc gccgccgttt tcaatcagcc
     3241 ccgccaaccg ttccaacgcg aaggcgaccg cctgcgcgcg gacggattcg cggttgccgt
     3301 caaaacggcg cattgcttcg caacttccgc ccggaaaggc aaacccgaac caaaccgtgc
     3361 cgacgggttt gctttcgctg ccgccgcccg gtccggcgat gccggaaata ccgacggcgt
     3421 aatccgcctg cgccacggct ttcgcgccgc gcgccatctc atagacggtt tggcggctga
     3481 ccgcgccgtg ttcgagcagg gtttcgggca acacgcccaa gcggtcttct ttggctttgt
     3541 tgctgtatgt tacaaaactc tggtcgaacc attgcgaact gcctgcaacg cttgtgaatg
     3601 cggcggcaag cattccgccc gtacaggact cggcacagct tacggtttga cgttttttcg
     3661 tcaggtttcg ggcgatggtg tgcagcgcgt ccattttccc actccccttt cagacggcgt
     3721 ttaagaattg atgatgtgta tgtcgcgttg cgggaacggg atgttgatat tgactttgcg
     3781 gaggttttcg accacttgtt cgttcaaatc gcattgcagc gtccagcggt ctgcttcgtt
     3841 tgcccaagcc cataatgtga tttcgatggc attgtcgccc aaggcggtga tgtaggcggc
     3901 ggcctgccgc tcttcgtttt gaacgctcaa ggggtgttcg acggcggctt tcaacaccgc
     3961 ctctttcgcc actttcaaat cgcagttgta atcgacgccg actatcactt gggcgcggca
     4021 aagcggcagg gtggaacggt tgacgatgct gttgcccatc accacgctgt tgggcagcac
     4081 gacttcttcg ttgtcggtcg tccgcaaaga agtctgcacc attttaatct ctcggacata
     4141 tccttcaaaa ccgccgacgc ggataaaatc gccgactttg aacgggcgaa acaggataat
     4201 cagcgcaccg gcggcaaaat tggacagctg gtctttcaag gacaacgcca ccgccaaacc
     4261 cgcgccgccg attaaggcgg ttacggatgt tgtggaaacg cccaatctgc ccaatgcggc
     4321 aataatcacc aaaatcaata agccgatatt ggcaacatta cacaaaaaac taatcagcgt
     4381 ggcatcgacc tgcgcgcgcg tcatcgccgc cctcatcacg gcgacaatgc gtttcgccgc
     4441 ccattttccg accaaaaaaa taagcagcgc ggcggcaagg ttcagcccga acgcccacgc
     4501 cttttcagcc agatgctccc aaccggaaac actgatcagg tgtaaaaaat caaattgttt
     4561 gaagtccatt gttttcctct tgatcgaaca tccgccccgt gcggcgtaat cggcacaggt
     4621 gtaaaaatgc cgtatgaagc cctgcggggc ggtatgtttg ttttatttca aaaccgttct
     4681 aatccaacgg gaaacccatc cgcgctggtt cgcatcaata tcgcgctgcg cgggggcggt
     4741 ggtgtgcgtc ggggcttggg cgaagcggaa cacgtcgggc aggcgcgtgt ttttgacggc
     4801 ggggtaaacc cacatttcgg acggaaccgc ctgctgcact tcccgacttt gcagccattg
     4861 caccagtttt gccgccagct tcggctgttt cgcgcccttc aagactgccg cgccttcgac
     4921 ctggcggaat acgccgcctt ttaaaaacag gttgcccgtc ggcggctcgc tgtatttgcc
     4981 tttggaaaaa tacacttccg ccgccgggct ggcggcataa ccgaccacca gcggatacgc
     5041 gccgccgttg tgcgaaaagt cggtgtaata cgcctcgctc cagcctttgg cgaccttcac
     5101 gccgttctgc cgcatctgtg cccaccattt gaacgcgcct tcttcgccca gaccgctgat
     5161 gttcgccatc aggaagccca gccccgggga cgacgtggcg ggagacggca cgaccaatag
     5221 gtttttatat tcggggcggg tcaaatcctg cagggtttgc ggcaggggca gctttttgcc
     5281 ttcaaaccat tttttgtcgt aattgatgga cacatagccg taatcgaccg ccaaagccga
     5341 aggcagcccg accgcgacgg gggcggattc gggttgcgct gccgccaaaa tgcccatttc
     5401 ccgcgccttg ccgatattgg cgttgtccaa accgtacacc gcgtcggcaa tcgggttggc
     5461 gcggctcaaa atcagtttgt tgagcatttc gttcgcgccg cccgcctgaa taatcgacac
     5521 cttcgcatcg tttgcccgct cgaagcgcgc aatcagccct ttgggcaggc tgaacgactt
     5581 atgcaccgcc agcctgactt ccgtctgcgc ctgcaggtat gccgaaaccg ccagcagcgg
     5641 cagcagccaa attttccgtt tcattccgag tcctcctcta ttcgctgtaa aataacattc
     5701 taacaaattt tcacggttca acatgcaaga aaacccgacc gtgtggctgt tcgacctcga
     5761 caacacgctg cacaatgccg atgccggcat cttcacactc atcaaccgcg ccatgacacg
     5821 ctatatggca cgccgcctca aactctccga atctgccgcg tccgacctgc gtcaagacta
     5881 ttggcaccgc tacggcgcaa cgctcgccgg actgcaaatc caccatcccg aaatcgacat
     5941 cgccgaattt ttgcgcgaaa gccatccgat cgatgcaatc ctgaccaggc tgcacggcat
     6001 gcctgaaaca caaaacaccc tgagccgcct aaaagggcgc aaggcggttt tttccaacgg
     6061 cccgtcgttt tacgtccgtg ccgttgtcaa cgcactcggt ttggaaaacc gtttcgacgc
     6121 gcttttcggc acggatgatt tcgggctgct gtacaaaccc aatccgcaag cgtatctcaa
     6181 tgtctgccgc ctgttggacg taccgcccga atgctgcatt atggtggacg acagcgcgga
     6241 caacctgcat caggcaaagg cgctgggtat gaaaaccgtc cggttcggtg caaaatccca
     6301 cgcgctgccc tttatcgatg cctccgtaag cgatatggcg caactggctc ggtatgcaga
     6361 aactttgtca gaacaccgcc aaaatcatta caataccccc taccccccga aaatacgaaa
     6421 gaatgttatg cgtaaaacct tccttatcct gatgactgcc gccgcccttt tgtcgggctg
     6481 cgcgtgggaa acttatcaag acggcaacgg caagaccgcc gtccgtcaaa aatatcccgc
     6541 cggcacgccc gtttattacc aagacggcag ctactcgaaa aatatgaact acaaccaata
     6601 ccgccccgaa cgccatgccg tgctgcccga ccaaaccgac ggttccgaac aagacgtacg
     6661 cggacagaac tggcaaaagc ccaaatttca aaaccgataa acctacccta tgccgtctga
     6721 agctgcttca gacggcattg cacaggaaac cgtcatgccg caaaacactt taaacatcgt
     6781 tatcctcgcc gccggcaaag gcacgcgcat gtattccaaa atgcccaaag tgctgcaccg
     6841 catcggcggc aagcccatgc tcgggcgcgt tatcgacacc gccgccgcgc tgaatccgca
     6901 aaacatctgc gtcgtcatcg ggcacggcaa agaccaagtt ttaaacgctg tcaaacgcga
     6961 tgtcgtttgg gttgaacaaa ccgaacagct cggtaccggc cacgccgtca aaaccgccct
     7021 gccccacctt tccgccgaag gccgcacgct ggtactgtac ggcgacgttc ccctgattga
     7081 cgttaaaacc ctcaaaaccc tgctcgaagc cgccggcaac gaagtcggtc tgttgaccga
     7141 cgttcccgcc gacccgacag gcttgggccg catcatccgc gacggcaacg gcagcgtaac
     7201 cgccatcgtc gaagaaaaag acgccgacgc cgcccaaaaa gccgtaaaag aaatcaatac
     7261 cggcattttg gttctgccca acgccaaact ggaaaactgg ctgaacagcc tctccagcaa
     7321 caatgcccaa ggcgaatact atctgaccga cctcatcgcc aaagccgttg ccgacggtat
     7381 taaagttcat cccgtccaag tgcacacctc ctacctcgcc gccggcgtga acaacaaact
     7441 ccagctcgcc gaactcgagc gtattttcca aaccgaacag gctcaagaat tgctcaaagc
     7501 aggcgttacc ctgagcgacc cggcacgttt cgatttacga ggccgtctga aacacggaca
     7561 agatgtcgta attgatgtga actgtatctt tgaaggcgaa gtcgaaatcg gcgacaacgt
     7621 cgaaatcggc gcaaactgcg tcatcaaaaa cgccaaaatc ggcgcaaaca gcaaaatcgc
     7681 ccccttctcc cacctcgaag actgcgaagt cggcgaaaac aaccgaatcg gcccgtacgc
     7741 ccgtttgcgt ccgcaagcca aactagccgc cgacgtacac atcggcaact tcgtcgaaat
     7801 caaaaacgcc gccatcggca aaggcaccaa agccaaccac ctcacctaca ttggtgatgc
     7861 cgaagtcggc agcaaaacca acttcggcgc aggtacgatt attgccaact acgacggcgt
     7921 gcacaaacac aaaaccgtca tcggcaacga agtgcgcatc ggttcaaact gcgtactggt
     7981 tgcccccgtt accctcggca acaaagtaac cacaggcgca ggcagcgcga ttacccgcaa
     8041 cgtcgaagac ggcaaactcg ccctcgcccg cgcccgccaa accgtcatcg aaggctgggt
     8101 gaggccggaa aaagacaaac aataaagcca tgccgtctga agccggtttc aggtttcaga
     8161 cggcatcaaa cccctccgat atcagataag gactgcaaac catgtcatta cccccatgcc
     8221 cacaatgcgc ctccgaatac acctatgaag acggcggaca atacatctgc cccgaatgcg
     8281 cccacgaatg gaatgaaacc gaatccgccg ccgaccttgc ggctcaagtg ctcgatgcca
     8341 acggcgcggc actgcaaaac ggcgataccg tcatcctcat caaagacctc aaggtaaaag
     8401 gcagctcgat ggtgatcaaa caaggtacaa aagtcaaagg catacgcctg caggaaggcg
     8461 atcacaacat cggctgcaaa atcgacggca gcgcgatgaa tttaaaatcc gaattcgtca
     8521 aaaaagcctg accgcccaaa aacaagaaac gccgtccgaa cccgtttggc aaggttcgga
     8581 cggcgttttt tatatggcgg atttatacgc ccagcagccc ttgccccaaa aagccgcctt
     8641 tttccacgcc gggcaagacg aagaaatagc cgccgccgaa ggggctgatg tattcttcca
     8701 gcggttcgcc gttgaggagg ttttgcacga agatgaatcc gtcggcaagg tttgcctgat
     8761 agcagacgaa caccagtccg acatcaagct gtccgcttga ggcaagtccg cgcgaatagc
     8821 tgtaggcgcg gcggaagagg cggtgttttt tgaggaattc gggatcgcgc ggattcgcca
     8881 ggcgtatatg gctgtctttg ggcgtggtat tcccctcggg gtctttggca aaatccagtt
     8941 ggtcggcttc ttttttgccg tccatcggcg caccgctgta tttgcgccgc ccgaaaatgt
     9001 cggtttgctc ttgaagcggc gtcctgtccc aaaactcgac aaagtggcgg ataaggcgga
     9061 ctgcctgata gctgccgttt ttcgcccact ccggttcgtc gaggctgttg gcggccacac
     9121 ccgtccacaa aacctcgtcg gcggttttgg ggtcggaaac tttggggttg cccgtgccgt
     9181 cgcggaagcc caacaggttg cgcgccgcca tcgcgccggg ttcggattta ggctgccacc
     9241 cgtcgatact ccagcggata acggcggttt ggacggtgtg tttgatgatg tcgcgcaggg
     9301 cggcttggca ggtttcgggg gtgaaggcac agatttgcag gctcaaatcg ccgtcgcacc
     9361 agcttttttg cagcttatcg ttggagaagt cgcgcatttc ctgcaaatga atcggttttt
     9421 tgtctttgag tccgaaccgg ccgtcaaaca ggctgctgcc cacccccacg gtaacggtca
     9481 acccgtcggg gttgaaggct ttgcccaaaa tgccgctgcc ggctggcgga agtttgtcgt
     9541 cgccgtcttg gtattcgccg ccttgggtga gaaactcgat gcgggcggtc agcgtgcgga
     9601 acaggttttc cagctgcttg gcactttgtg cggttacgtc gaaggcgcac ataatcgaaa
     9661 acgcctgctg cggcgtaacg atgcctgcct gatgttcgcc gtagcaggga taggcttggg
     9721 gcgagtgttg gctttcggcg gtgcgttcgg cggtttcgcc ccgttttttg ccgccgagat
     9781 aacctccgat tgcgccgact gctccggctg cgatcgcggt tttaaaaaga gtgcgcctgg
     9841 tcggttgtgc gggttggttt ttgctcatgg tgtttccttc aatatccgtg cagcaggatg
     9901 ggtatccctg cacgccgttt ttccattaac cgatagcggc aggttgaaat cccgccctgc
     9961 aatatatggc ggatatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca
    10021 gtataaatag tacggcaagg caaggcgagg caatgccgta ctggtttttg ttaatccact
    10081 ataaaacttc agacggtatc tgccgccaaa taccgtctga acgcttgcgg cttatttcaa
    10141 gccgagtatg ccgcgaagtt gggcaaggtc ttcggcaagc gcgttaatag aggcctgtaa
    10201 cgctttgcgg tcggcttcgc ccagcttgtc gtaggtttca aaaccgtctt tagtccggta
    10261 tttcgccaga atttcgttga cctgtttgaa gttggtatcg gttttttcca acaaggcttt
    10321 gtttttggcc tcgatcagcg gacggaacaa atcgacgatt tttttagatc cgtccacatt
    10381 ggcttggaag tcgctcaaat cggtgtggct gtaccggtct tcttcgccgc tgattttact
    10441 gcccgccact tcttcaatca gttcggacac gccgccgacc accttgcccg gaggaaacgc
    10501 caatgcgtcg atttcttttt gcagggcttc gacatcggtc atcagtttcg ctgcaatttc
    10561 cttcacgccg gacacgtctt tttccaccca aagggcgtat tcgatacggt ggaagccggt
    10621 aaatccggca tctttcgcgc cgtctttgaa gtcgtcttca cgcgcatcga tgacggggtc
    10681 gagttcgctg aaaagctcgg caatcggttc gatgcgttcg taatggacgc gggtgtcggc
    10741 aaacagggat ttcgcctttt caatgtcgcc tgctttgacg gcttcggtaa aggttttggt
    10801 tttcgccacc agctctttaa cctcgccttg aacgtaggct ttatagtcgg cgagcggttg
    10861 ggacagtttt tccaaatccg cttcgttggc ggtgtcttta aagccgctgt cggttaccac
    10921 cagcttgccg cgcggattgg tcaaaagacc gcaagtcatt tcgtattcgc ccggcaacag
    10981 ggtgacggtc attttatcgg aaagtccggg ggcgatgttt tcgcgctcgt ccaccaccat
    11041 cacgcctttc aggatttccc attcgagctt gcggccgctg ttgtttttaa tattgaacac
    11101 aacctgtccg ctcggcacgg tcagttccat cggttcgcag gcattgtcgt tgacggcgat
    11161 actgaccgaa ccgccctcgt tggcggtttg cgcctcaccg gacgcggccg gcgcagcttt
    11221 ctccgcctcc ggcggctggc acgcggtcaa acctaaggca agcatcacgg acaatgcggt
    11281 caaattgaat tttctcattt cagctcctct ttacgggtta aagtttcaga cggcctgctg
    11341 ccgcacaaaa accaagtcat gacgggaata aggtacagca gccaaaccaa ggcctcgccc
    11401 tgcgtcggat ggtcggtata gccgaaaaat ccgccgagca gcacacctaa cggactgtct
    11461 tcgtgcaaat attttgatga gtcgaacaca atgtcctgaa gcgcgttcca aatgcctgct
    11521 tcgtgcagcg cgcgcagcga gccggcaagc aggccggcgg caacgacaat cagaaacgcc
    11581 cccgtccaac ggaaaaactt cgccagattc aggcgcatcc caccctgata aatcaacgcg
    11641 ccaatcacgg cggcagccaa aacccccgct accgcaccgg ccggcatctg ccaagtcggg
    11701 ctctgtttga atacggcaag cagaaaaaaa acgctctcca aaccttcgcg cgccacggca
    11761 agaaacgcca taccgaccaa ggcccatcct tgtccgctgc cccggttcaa agccgcctgc
    11821 acagaatcct gaagctgctg cttcatcgaa cgcgccgcct ttttcatcca taaaaccata
    11881 taagtcagca tcgcgacggc aaccaggccg ataatgccga cgacgaactc ctgctgcttt
    11941 tggggaatct cgcccgttgc cgaatggatg ccgtacccca gccccaaaca catcaaagaa
    12001 gcaaggacga ccccgaacca gaccttaggc atcagtttgg aatgtccgga ctgtttcaga
    12061 aaaccggcaa cgatgccgac aatgagcgcg gcttcgatac cctcgcgcaa cataattaaa
    12121 aaagcgacca gcataaacgc gaacgaacaa ggatgatgaa taatatatta tcggaatatt
    12181 ttcattgctt gtaaatacaa atgcaagtta tttttatctg cagtaccgcg cggcggaaag
    12241 ttccgcaggc tgcagctgcg ccctgtgtta aaatcccctc tccacggctg ccgcaacgcc
    12301 gcccgaaacc atctttctta ttactgccgg caacattgtc cattatgaaa aaatacctgt
    12361 tccgcgccgc cctgtacggc atctccgccg ccatcctcgc cgcctgccaa agcaagagca
    12421 tccaaacctt tccgcaaccc gacacatccg tcatcaacgg cccggaccgg ccggccggca
    12481 tccccgaccc cgccggaacg acggttgctg gcggcggggc cgtttatacc gttgtgccgc
    12541 acctgtccct gccccactgg gcggcgcagg atttcgccaa aagcctgcaa tccttccgcc
    12601 tcggctgcgc caatttgaaa aaccgccaag gctggcagga tgtgtgtgcc caagcctttc
    12661 aaacccccgt ccattccttt caggcaaaac agttttttga acgctatttc acgccgtggc
    12721 aggttgcagg caacggaagc cttgccggta cggttaccgg ctattacgaa ccggtgctga
    12781 agggcgacga caggcggacg gcacaagccc gcttcccgat ttacggtatt cccgacgatt
    12841 ttatctccgt ccccctgcct gccggtttgc ggagcggaaa agcccttgtc cgcatcaggc
    12901 agacaggaaa aaacagcggc acaatcgaca ataccggcgg cacacatacc gccgacctct
    12961 cccaattccc catcactgcg cgcacaacgg caatcaaagg caggtttgaa ggaagccgct
    13021 tcctccccta ccacacgcgc aaccaaatca acggcggcgc gcttgacggc aaagcaccga
    13081 tactcggtta cgccgaagac cctgtcgaac ttttttttat gcacatccaa ggctcgggcc
    13141 gtctgaaaac cccgtccggc aaatacatcc gcatcggcta tgccgacaaa aacgagcatc
    13201 cgtatgtttc catcggaaaa tacatggcgg ataagggcta cctcaaactc ggacaaacct
    13261 ccatgcaggg cattaagtct tatatgcggc aaaatccgca acgcctcgcc gaagttttgg
    13321 gtcaaaaccc cagctatatc tttttccgcg agcttaccgg aagcggcaat gacggccctg
    13381 tcggcgcact gggcacgccg ctgatggggg aatatgccgg cgcagtcgac cggcactaca
    13441 ttaccttggg tgcgccctta tttgtcgcca ccgcccatcc ggttacccgc aaagccctca
    13501 accgcctgat tatggcgcag gataccggca gcgcgattaa aggcgcggtg cgcgtggatt
    13561 atttttgggg ttacggcgac gaagccggcg aacttgccgg caaacagaaa accacgggat
    13621 atgtctggca gcttctgccc aacggtatga agcccgaata ccgcccgtaa accggaaaac
    13681 aatgccgtct gaagcccatc cctaggcaat cgggcgcatt aaggcgaagt tttgcgatac
    13741 cgcaggcaca aaattccttt cttatttgac ggaattatag tggatcaaat t
//
LOCUS       DAWFAD010000044        13620 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWFAD010000044 DAWFAD010000000
VERSION     DAWFAD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13620
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.044"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(298..633)
                     /locus_tag="WGO49_000955"
     CDS             complement(298..633)
                     /locus_tag="WGO49_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ0306077.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARGKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(713..2842)
                     /gene="dnaX"
                     /locus_tag="WGO49_000956"
     CDS             complement(713..2842)
                     /gene="dnaX"
                     /locus_tag="WGO49_000956"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ0306078.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLHNMTSQQVADHLAHVLDSEKIAYEPPALQLLGRAAAGSMRDAL
                     SLLDQAIAMGSGSVAEQDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQHLALIQAVPSALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDTNAVIENTELKSPSAQTAEKETAVK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAGPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNVTDAPLSEVPSENPIQATPNDEAVETETF
                     AHEAPAEPFYGYGFPDNDCPVEDGAEIPPPDWEHAAPADTAGGGADEEAEAGGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWRDEAGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1614..1653)
                     /gene="dnaX"
                     /locus_tag="WGO49_000956"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2937..4913)
                     /gene="mutL"
                     /locus_tag="WGO49_000957"
     CDS             complement(2937..4913)
                     /gene="mutL"
                     /locus_tag="WGO49_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ0306079.1"
                     /translation="MSRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGGGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSDNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSQQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVTPAPMPSENNSENLFDRASNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSPSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGNLQSQRLLIPVTFAASHEECAALADHAEALAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLNEFAQVGSSQTIEEHENHILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            5057..5242
                     /locus_tag="WGO49_000958"
     CDS             5057..5242
                     /locus_tag="WGO49_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ0306080.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5253..5921
                     /locus_tag="WGO49_000959"
     CDS             5253..5921
                     /locus_tag="WGO49_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ0306081.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLMRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVSFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIVQGEAGSYDLALIDADKPPTPQYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDPRIVPITLPVGDGLTL
                     LLKK"
     gene            5921..6637
                     /locus_tag="WGO49_000960"
     CDS             5921..6637
                     /locus_tag="WGO49_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ0306082.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRLEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            6686..7171
                     /gene="purE"
                     /locus_tag="WGO49_000961"
     CDS             6686..7171
                     /gene="purE"
                     /locus_tag="WGO49_000961"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ0306083.1"
                     /translation="MTQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQAVLSMELEQ
                     I"
     gene            complement(7354..8808)
                     /locus_tag="WGO49_000962"
     CDS             complement(7354..8808)
                     /locus_tag="WGO49_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ0306084.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPTEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPILGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8805..9506)
                     /locus_tag="WGO49_000963"
     CDS             complement(8805..9506)
                     /locus_tag="WGO49_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ0306085.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEVERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            complement(9503..10282)
                     /locus_tag="WGO49_000964"
     CDS             complement(9503..10282)
                     /locus_tag="WGO49_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ0306086.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            10566..11972
                     /locus_tag="WGO49_000965"
     CDS             10566..11972
                     /locus_tag="WGO49_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0306087.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIAADL
                     NESPLNMQLAVISYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRVYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSPLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTPS"
     gene            12084..13610
                     /locus_tag="WGO49_000966"
     CDS             12084..13610
                     /locus_tag="WGO49_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0306088.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQTG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPKYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIEGLL
                     "
BASE COUNT         2765 a         3675 c         3828 g         3352 t
ORIGIN      
        1 acggggttgg attcccgctt tcacgggaat gacgaagttt cagacggcat ttaagcggta
       61 cggatgtgta aataatggta gggtgggctt cagcctgctg attcccgcta ttcttgccgt
      121 ttttgcgttc ttatcattct cactgttttt accgttcacg ccgaatcctg caaactgatg
      181 ccgtcattcc cgcgaaggcg ggaatccaga cccgtcggca cggaaattta tcgagtaaaa
      241 cggtttctta gattctacgt tctagattcc cgcctgcgtg ggaatgacgg tcgggtatca
      301 gcggaagaag tcgcccactc cggggggtag accttgcgtg aatgcgccca ttgttttgtt
      361 tgcggtttct tcggctttgc cccgggcgga tttgagggcg gcgaggatga ggtcttcgag
      421 catttctttg tcgtcggcgg cttcttgaat caaatcgggg ctgatgtcga ttttgcgtac
      481 ttcgtgcgcg caggtcattg tgattttgac caggccgttg cctgcttcgc cttcgatttc
      541 ggtttcggca agtttggctt gcgctttttt catattttcc tgcatttgct gcgcctgttt
      601 catcaggccg cctaatccgg cttttccgaa catactgaat actcctgtct gttttgattg
      661 aatgagggaa acggcaatgc cgtccgaagg gttcgggcgg cattatatct gtttatggcc
      721 ggtttgctgc caattccagt gattcgggct gccattgcgc gccgaatgct tggaggattt
      781 tttgtgcggc ggggtcggct tcgagcaatg cttgtgcttt ttgcctgtct tcggcttgga
      841 cgcgcttgtc ctgcatcgcg ggggtttccc tgccggcttc gtcgcgccag tcttgggttt
      901 gcagggtgag ttgcagtccg taggcttggg caagggtgtc gcggattttg tcgagacgtt
      961 ttttgtcggc ggtggcgcgg gcttcggcgg tcatcgccaa aaccatcagg ccggtgtcgg
     1021 gatggtattc cgtccacgcg gagtgttgcg ccggcatttg cgccgcgccg agtttgcggg
     1081 cgaaatgccg gacgatggct gcccagtttt cggtggaaaa ttcgggcggc ggggcggacg
     1141 gcgtgttgtt tccgccgatg ccgcctgctt ctgcttcttc gtccgcgcct ccgcctgcgg
     1201 tatcggcagg ggcggcgtgt tcccaatcgg gcggcgggat ttctgcgccg tcttctacgg
     1261 ggcagtcatt atccggaaag ccgtaaccgt agaaaggttc tgcaggggct tcgtgtgcaa
     1321 atgtttccgt ttcaacggct tcatcattcg gtgttgcctg aatggggttt tcagacggca
     1381 cttcggacaa gggggcatcg gttacgttgt cggctgcctt gttcttggaa acttggtttt
     1441 cgggcggcgt ttcggcttcg gatgccgtct gaatgctttt tgccgacgtt tgcgccgtgc
     1501 cggctgcggc ttgggcttcg tctggcgcgt cttcccaagg cggaacatcg ttgttttctt
     1561 gattggaaac gggtccggca gttttgcctt cagacggcat tgccgctgcg gatgccgtct
     1621 gaacgggtgt ttgggcggtt tctgcttcag ggcgcggttg gggctttttt acggcggttt
     1681 ccttttcggc ggtttgtgcc gatggggatt ttagttcggt attttcaatc acggcgtttg
     1741 tatcacacga agctgccgcc aagggcgcaa acgccagcat acgcagcagg gtcatcataa
     1801 agccggcgta ttcgtcgggg gcgaggctca ggtcgcgttt gccgtggacg gcgatttggt
     1861 agtaaagctg gatttgttcg ccgcttatgg tttgggcgag gcggtgcaaa atatcggaat
     1921 cggggtcgtc gtgcgccaag gcggagggca ccgcctgtat cagggcgagg tgttgcagca
     1981 gtatggcaag ttcgcccaag gcgttgtcaa agccgacggc acacgccgcc atttcctgcg
     2041 ctttggcggt cagggctgcg ccgtcttggt tgatgatgcc tgtcaagagt tcgtaaaggt
     2101 attgtttgtc aaccgcaccg atcatttggc ggacgtcttg ttcggcaacg ctgcccgaac
     2161 ccatggcgat ggcttggtcg agcaggctca aggcatcgcg catcgatccg gcggcggcgc
     2221 gtcccaaaag ttgcagggca gggggttcgt aggcgatttt ttcgctgtcg aggacgtggg
     2281 cgaggtggtc ggcaacctgc tgcgaagtca tattgtgcaa gacaaattgc aggcagcggc
     2341 tcaagacggt aacggggact ttatgcggat cggtcgtggc aaggatgaat ttgacgtgtt
     2401 cgggcggctc ttccagcgtt ttgagcatag cgttgaacgc gcttttggaa agcatatgca
     2461 cttcgtcgat gatatagact ttgtatttgc cggcagtcgg tgcatattgg gcgttttcca
     2521 atacttcgcg gatgttgtcg atgcctgtgt tggaggcggc gtcgatttcg agcaggtcga
     2581 catagcgtcc ggcatcgatt tgggtgcagc tttcgcacac gccgcaaggt tcgccgtgtt
     2641 gcgcgttttc gcagttgagg cttttggcaa ggatgcgggc gatggtggtt ttgcctacgc
     2701 cgcgcgtacc ggtcagcagg taggcgtggt gcagcctgcc ttcgtccaag gcgttttgca
     2761 aggctttgac gacgtgttcc tgaccgacta agtcggaaaa ggttttgggc cgccattttc
     2821 gggcgagaac ttgataggcc atgtttttct cttggtttcg gtcgtgatgt ttctgtcggt
     2881 gcgtcggaat gccgtctgaa cggcggcttc gggcggcgta ttctagcact ttcggtttat
     2941 tgtccgcgca agaacagtgc gtccaattct ttcaaagtca gtttgaccca agtcggcctg
     3001 ccgtggttgc actggttgct gcgcggcgta ttttccatat cgcgcagaag ggcgttcatt
     3061 tcgggcaggg tgagccggcg gccggcgcgg atcgagccgt ggcaggacat ggtggcgagg
     3121 atgtggtttt cgtgttcctc gatggtttgg ctgctgccga cttgggcgaa ttcgtttaat
     3181 acgtcgcggg cgagggagac gacatcggat ttgcccaaca ttgcgggtgc ggcgcggacg
     3241 gcgagggtgt tgccgcccat atcggacagt tccagcccga agcctgccag tgcttcggcg
     3301 tggtcggcaa gggcggcgca ttcttcgtgg gacgcggcaa aggttacggg aataagcagg
     3361 cgttggcttt gcaggttgcc gttttcctga cgttggcgtt tcattttttc gtagttgacg
     3421 cgttcggcgg cggcgtgcat atcgatgagc aacaggctgt cttcggcttg ggcaagaatg
     3481 tagatgccaa gtaattgggc aatggcgaaa ccgagcggcg gcagttcgga tggggacggg
     3541 atgccgtctg aaagcggcgt atctgtttgg ggagcaggcg tttcagacgg catattgccg
     3601 aaacgtgcct gctcgaatcg gcttaactca aggtcgatgt cgtcggtttt tttgtaaagt
     3661 tcggcgtaag tattcattgc cgcgcggctt tcgcgcaggg acaggctgcg ttgttgcggc
     3721 gcatatgcgg actgataggg catgggcgcg gttttgcctg atgaaccaaa ggcattgtgt
     3781 gtatctgatt tgttgcctgt cgggtagttg gatgcgcgat caaacagatt ttcgctgttg
     3841 ttttcagacg gcattggtgc aggcgtaacg ccggtaatgt catgcaacac ttcgcctgcg
     3901 ttgctgacgc tttcggtcag gtcggcgcgt gtgtcggcaa gggctttgtt gagcgtgtgg
     3961 aacacaagtt ggtgcacctg ctgactgtcg cggaagcgga tttcggtttt ggtcgggtgg
     4021 acgttgacat ccacggcttc gggcggcagg tcgagaaaga ggacgaaagc aggcgtcagt
     4081 gcgttgtgca atacgtcgcg gtatgcctgc ttgacggcat ggagcatgac tttgtcgcgg
     4141 acaaaacgat ggttgacgaa gcagtattgt ttgtcggttt tacctttggc gaaagtcggt
     4201 ttggcaatcg cgccatagag ccgcaatgcg ccgttgtcgc tgtcaatttc caatgatgcc
     4261 gtctgaaaat catcgccgac aatggcggca atccgttcat gcaggctttg tgcagggagt
     4321 ttgaacactt gtttgccgtc gcgtttgagc gagaaggcaa tgtgcggatg cgccagcgcg
     4381 aggcgttcga gcatagtggc gcagtgggcg tattcggtgg cttcagactt gaggaacttg
     4441 cgccgcgcag gggtattgaa gaagagttcg gcggcttcga tggtggtgcc gacggggtgg
     4501 gcggcggcgg tggggctgct gagtttgccg tcttcggctt tgacttgggt tgcgtgcgaa
     4561 ctgtcttctt gacggctggt cagggtcagg cggctgacgg aggcgatgct tgccaagcct
     4621 tcgccgcgaa agcccatgct ggcaacgtgt tccaaatcgt ttaaggtttt gattttgctg
     4681 gtagcgtgtc ggtggagcgc aagctcgatg tcgtcggggt ggataccgcc gccgttgtcg
     4741 ctgacgcgaa tcaggcggat gccgccgccc gccagctcga catcaatcgc cgttgcgcct
     4801 gcatcgatac tgttttcaac aatttctttc aaggcgttgg cagggcgttc gactacttcg
     4861 ccggcagcga tttggttgac aagatggtcg ggcagggcgg caattcggga catggcatgg
     4921 gcttccgttt caaaaaacgc tttattctat aataaaaccc cttatctttc tgcccgtatt
     4981 cttgataaaa gccgtttatc ccgtttggaa aataccagta taatcacgcc gtatcccgta
     5041 aaattggagc gcaaagatgt accactacca atccgaagcc acacaattcc tcaaccgcct
     5101 gattgaagaa aaacctgagt tggaacagca gcgtttggaa aaccgggggc ttttgtggga
     5161 tgtcgaactc aatcccgaag aacaggaaaa cttcgaggcg gcaaaagtag cgaaaaaacc
     5221 ttatacctat taccaagact gaacccgccg aaatgacgac ccatctgtcc aatgtcgccc
     5281 ccgacctgca aaactatttg aacgccatcg gcgaacctga acatcccgtt ttgatgcggc
     5341 tgcgcgagaa gaccgggcat caccgtatgg gcaaaatggc gattgcgcgc gaacaggcgg
     5401 cggttttggt ttggctggca aagctgatcc gtgcggaaaa atatctggaa gtcggcgtgt
     5461 ttactggata cagcagcacc gcgcttgcat tggcgttgcc cgaacacggg cggattaccg
     5521 cctgcgacat caatgtaagc tttaccgata cggcgcgtca ggtttggaac gaggcaggtg
     5581 tggcacataa aatcagcctg cacctgcaac ccgcattgct gacattggat gatttgattg
     5641 tacagggtga agccggaagc tacgatttgg cactgataga cgcagacaaa ccgcccacgc
     5701 cgcaatattt cgagcgttgc ctcaaactcg tccggcaagg cggcatcatt gccattgaca
     5761 atattttgtt gaacggaagg gtgatgcgcg aagcggcttc cgacgcgccg cccagtgtcg
     5821 gcatcctcaa agatttcaat caaaacctgc cgaacgaccc gcgcatcgtc cccatcaccc
     5881 tgcccgtcgg cgacggtttg accctgcttc tgaaaaaata atgaagacca aattaccgct
     5941 ttttatcatt tggctgtccg tatccgccgc ctgttcttcc cctgtttccc gcaatattca
     6001 ggatatgcgg ctcgaaccgc aggcagaggc aggtagttcg gacgctattc cctatcccgt
     6061 tcccactctg caagaccgtt tggattatct ggaaggcaca ctcgtccgcc tgtcgaacga
     6121 agtggaaacc ttaaacggca aagtcaaagc actggagcat gcgaaaacac acccttccag
     6181 cagggcatac gtccaaaaac tcgacgaccg caagttgaaa gagcattacc tcaataccga
     6241 aggcggcagc gcatccgcac ataccgtcga aaccgcacaa aacctctaca atcaggcact
     6301 caaacactat aaaagcggca ggttttctgc cgctgcctcc ctgttgaaag gcgcggacgg
     6361 aggcgacggc ggcagcatcg cgcaacgcag tatgtacctg ttgctgcaaa gcagggcgcg
     6421 tatgggcaac tgcgaatccg tcatcgaaat cggagggcgt tacgccaacc gtttcaaaga
     6481 cagcccaacc gcgcccgaag ccatgttcaa aatcggcgaa tgccaataca ggttgcagca
     6541 gaaagacatt gcaagggcaa cttggcgcag cctgatacag gcttacccga gcagcccggc
     6601 ggcaaaacgc gccgccgcag ccgtacgcaa acgatagttt ttccattttt ccgcttttta
     6661 ccccatccgc accaaggaga gatttatgac acaaatcggc atcatcatgg gcagcaacag
     6721 cgattggccc gttatgcggc aggcagcgca gtttcttgaa gagttcggcg tagaatatga
     6781 ggcgcgcgtc gtttccgcac accgcactcc ggatttaatg ttccaatacg ccgaaaccgc
     6841 acgggcgcgc ggcatcaaag ccatcatcgc gggtgcgggc ggcgcggcgc atttacccgg
     6901 catggttgcc gccaagacca ccgtccccgt tttgggcgta cccgtcccca gcaaatacct
     6961 gcgcggcgaa gattcgcttt tatcgattgt acaaatgccc aaaggcgtac ccgtcgccac
     7021 attcgccatc ggcgaggcag gcgcggcaaa tgccgcattg ttcgccgttt ccatgctggc
     7081 caacgaaaac cccgaactgg cgcaaaaact ggcagacttc cgagccaaac aggaacaggc
     7141 tgttttgagt atggaattgg aacagattta aaacccgccg tccgcgaata tatagtggct
     7201 taaatttaaa ccagtacggc gttacctcgc cttgccgtac tatttgtact gtctgcggct
     7261 tcgtcgcctt gtcctgattt ttgttaatcc actatacaaa tgccgtctga agccctgttt
     7321 cgggtttcag acggcatttt tgcaaacaac tgtttatgcc tgttcttttt ggcgcatttt
     7381 ttctgccatt agttcgtgca gggttttctt cgccggtttc atcggcacgc ggttttgcgt
     7441 ccaacccaac tgtttgcgcg gggtcaggtt gcggaacttg gtggctgccc aaccgaaggc
     7501 gcggtaggtt ttgctgccgc tgaaaatacc gttgaatgtg cgccacgcca tttgttcgcc
     7561 gaaggtatgc gatgcgcctt gccctaggat ggggtgtggc acggtttcgg tcggcgaacg
     7621 ttgcgcttca acgcgcaaac gctgcatttg ttcggtaatc gggatgcgta ccggacagac
     7681 ttccacacac gcgccgcaca tggtacaggc ggttggcaag tcgcgggtgg catccagacc
     7741 caacaggtga ggggaaataa tctcgccaat cgggccggga taggttgtgc cgtatgccgc
     7801 gccgccgata cgggtataaa ccgggcaatg gttcatacac gcgccgcaac ggatacattg
     7861 caaggtgcgg cgcatttggt cttcggcata ggcttggctg cgaccgttgt caagcaggac
     7921 caagtgcatt tcttgaggac cgtccagctc ttcgctacgg cgcgggccgg taatcatatt
     7981 gaaataagtg gtaatgttct gaccaatggc agaacgcggc agcaggctgt acaagggtgg
     8041 gatgtcggac aatttcgcca ccactttttc aatgccggta atagcgatat gcacgggcgg
     8101 tacggtggta ctcaagcgac cgttgccttc gttttccacc agacacagcg tacccgtttc
     8161 ggcaacagca aagtttacgc cactcaaacc gacatcggca gtgctgtaaa tatcgcgcag
     8221 ggctttgcgg gcgaagccgg tcagttggtc cacgtcgtcc gtaagcggag ttccaaggtt
     8281 ttggtggaac agttcactca cctgttcttt ggttttgtga atggcgggca tcacgatatg
     8341 ggtcggtttt tcgcctgcca tttggacgat gaactcgccc aagtcgcttt ctaccgcttt
     8401 aatgcctttt gcttcaagat aatggttcag ctcgatttcc tcgctgacca tcgatttgcc
     8461 tttgaccatc agcttgccgt ttttggctgt gatgatgtcg tggataattt ggcaggcttc
     8521 ggtcggggtt tctgcccagt gcactttcac gcccaactta gtcaggtttt cttccagctg
     8581 ctccagcagg gctggcaatt tagacaatga gcgctgacgg acgtgttcgc acaaatcgcg
     8641 caggctttgc agctcttctt cgtcggtcaa aacggctttg cgtttggtca tcagcatatc
     8701 catcgcggta cgcaggcttt tgcgcaaagg cttgtcttga agggaaattg cggcgttttg
     8761 cttgaaagtt tccggcttca tgtgaaattt gatggtttgc gtagtcatgc gttttcctcc
     8821 aaatcggcag gggaaatgtg gtcgggcagg atggcgagga tgaccaaatc gcgcgggccg
     8881 tgcgcgccgt aagcaagcgt cagttggatg tctgcggttt tggacgggcc ggaaatcagg
     8941 aatacattgg tcggcatacc gttttccacc agtttttcgc cttcgacggc attatgaaac
     9001 tcgttgtaca tcttggacgt atcgaacagg cagaaatgca cgggcggaac gagactcaaa
     9061 gtacgcggtt cttcggggct ggaaaacagc atcagcgtgc cggtgcgggc gatgccgcat
     9121 tgcgcgccgc tgaagcccgc atcgatgttc gtgaaaaact cggttttcca agtatcgatt
     9181 tcgcgctcga aggcaatcgg ttcgatattg ctgtccgcca atgcggcacg ggcaatttgt
     9241 ccgtgttcgg tcgccaaggg cagcaggatg tttttcaaac ccttgccttc tgccgcttcg
     9301 cggaaaacct gcatccaatt gcttttcgtc acccaataaa tttcggtttt gaccgcacgc
     9361 atagcggcag cccaatgttt cagacgctca acttcgctgc cccaagaaac acccatttca
     9421 cggtaataat caaaaaccgc aggttcttcc atcggcaatg cgtcggcttt tttcagtttt
     9481 gccaaaatat tttcgcgcgc gctcatgctt tgcctccggt acgttccaac aagaaggatg
     9541 cgatatgttt cggacgcggc atattcggtt cgtccttagc aattttgccg ccgatgttca
     9601 tcatacagcc gcagtccgcg ctgatgattt cggttgcgcc ggtttctttc agcgcggcga
     9661 ctttgtcggt taccatcgcg ccggaaatat cggcttgttt gacggagaat gtgccgccga
     9721 agccgcaaca ttcgctttcg tggtcgtgga cgatgcgttc gacgttttcc ataccgtcaa
     9781 tcagttgcca gcccgagaga tggacattca tttcgcggcg ggcggcgcag gaagtgtgaa
     9841 cggcgacttt gagcggttcg cccttgtctt cgggtttgaa accgatggca agcaggaaat
     9901 gggtaaactc gatgatgcgg ccggcgcaat ccacagccct ttcctcgtac tcgctgcctt
     9961 taaacagcgt cggccaatgg tgcttcatca taccgccgca cgaaccggac ggtacgacaa
    10021 tcggccactc ctcggggaaa aggtcgagtt gcgctctggc gacatcaaag gcttcggtcg
    10081 gatggccgga tgaataggcg ggctgaccgc agcagctttg cgccatcggg aaatggacgc
    10141 gtatgccctg ttgctcgatt agggtaatgg catccatgcc tgcttcgggc ataaaaagat
    10201 caaggacgca agtgccgaaa aaatagacat cggtcggatt gctgtcgtat cggatgattt
    10261 ttgggggaat ggttgcgctc atgttttttg ataacgggaa acccgttttt ttctgtagaa
    10321 aggtaagcgt ttactttaag taattgactg ttgcgggtca agtctaattt taaaaaataa
    10381 tccggttttt cttacaaact accccataac gcttactgta ccttaatctg atagttttcg
    10441 ataataatta tcattacaat gcaatgccgg ttcgtttgct tgtgaacatt caagatgccg
    10501 actctgacgg cattcagaca gcatctgaaa acaataacgg cacaaggatg gggtacaatc
    10561 gctctatgga aaaaaacacc ctatccgccc gcgcaccatc cccttggctg ccgcttctgc
    10621 tggcaattgc catttttatg cagatgttgg atgcgaccat tttaaatacc gcactgcctg
    10681 aaattgccgc cgacctgaat gagtcgccat tgaacatgca gctggcggtt atttcctaca
    10741 cgctgacggt tgccctgctg attcctttga gcggttattt ggcggacagg ttcggaacga
    10801 aaaaagtctt tttcggttcg attgccgttt ttatgctcgg atcggcattg tgcgccgcat
    10861 cgggttcgct gtttgaattg acgctttccc gtgtcgttca gggcatcggc ggttcgatgc
    10921 tggttccgat accgcgtctg accatcttgc gtgtgtacga caagtccaag ctgctcaacg
    10981 ccatcaatta tgcggttatg cccgcattaa tcggaccggt tttagggcct ttggcgggcg
    11041 gttatctggt cgaatacgct tcgtggcact ggattttcct gctcaacctg cccatcggtc
    11101 ttctgggttt catattggga cgcaacatca tgcccgatat taaaggcagc aatatctctt
    11161 tagacttcaa aggctatctg attttttctg ccgccgcgtg cctcttgtta ctttcggcag
    11221 aaagcctgtc gcacgcgctg cctccgtatt ttgcactgtt gccgctgtgc ggcggactgc
    11281 tgtttgcacg ccgttatttc cgacatatga aaaccgcgtc caaaccgatt tattccgccg
    11341 acctgtttct gatacgcact ttccgtctag gactggcggg caatctgttc agccgtctcg
    11401 gcatcagctc gattcctttt ctgatgcccc tgatgtttca agtcgctttc ggcttcggcg
    11461 caagcctgtc gggttggctg gtcgcccccg tcgccctgtc ttcgctgctg gtcaaaccgc
    11521 tgattgcacc gctcatgaaa cgtttcggct accgcacggt actgctttgg aacaccaagc
    11581 tgcttgccgc cttcatcatg ctgctcgccc tacctgatgg aaactcgccg ctgtggattt
    11641 ggattttcct ctcgctggcg atcggcgcgt gcaactccct acagttttct gccatgaaca
    11701 cactgaccct cgccgatttg cgcccgcaac agaccggcag cggcaacagc ctgatggcgg
    11761 tcaaccaaca gcttgccatc agcatgggca ttgttgtcgg cgcattaatc cttaaaaact
    11821 ggacatttct gataccggct tcttcaggtc tgcattccgc cttccgtatg accctgctca
    11881 gtatcggcgg cattaccctt gcctcgtcgt tggttttcaa gcgtctgcac gtttcagacg
    11941 gtgcaaacct gacacggaac acaccgtcct aaagcggtcc gcacgcaaaa cttttacccg
    12001 tttcaacgtt tggattatga ttccgcactt ccatgcgcac caaccccaaa acacaggcaa
    12061 tgccgtctga aaccatatcc ctgatgaaaa cacgcagcct aatttccctt ttatgcctcc
    12121 ttctctgttc atgttcttca tggttgcccc cactggaaga acggacggaa agccgtcatt
    12181 tcaatacttc caaacccgtc cgcctggaca acatcctgca aatccggcac acccctcata
    12241 ccaacgggct atccgatatc tatctgttga acgaccccca cgaagccttt gccgcccgcg
    12301 ccgcccttat cgaatctgcc gaacacagcc tcgatttgca atactacatc tggcgcaacg
    12361 acatttccgg ccgactgctg ttcaacctcg tgtaccttgc cgcagaacgc ggtgtgcgcg
    12421 tacgcctgct gttggacgac aacaacacgc gcggattgga cgacctcctg ctcgccctcg
    12481 acagccatcc caatatcgaa gtgcgcctgt tcaacccctt cgtcttacga aaatggcgcg
    12541 cactcggcta cctgaccgac ttcccccgcc tcaaccgccg catgcacaac aaatccttta
    12601 ccgccgacaa ccgcgccacc atactcggcg gacgcaatat cggcgacgaa tacttcaaag
    12661 tcggtgagga caccgttttc gccgacctgg acatcctcgc caccggcagc gtcgtcggcg
    12721 aagtatcgca cgacttcgac cgctactggg caagccattc cgcccacaac gccacgcgca
    12781 tcatccgcag cggcaacatc ggcaagggtc ttcaagcact cggatacaac gacgaaacgt
    12841 ccagacacgc gctcctgcgc taccgcgaaa ccgtcgaaca gtcgcccctc taccaaaaaa
    12901 tacagacagg acgcatcgac tggcagagcg tccaaacccg cctcatcagc gacgaccctg
    12961 caaaaggact cgaccgcgac cgccgcaaac cgccgattgc cgggcggctg caagacgcgc
    13021 tcaaacagcc cgaaaaaagc gtctatctgg tttcacccta tttcgtcccc acaaaatccg
    13081 gcacagacgc actggcaaaa ctggtgcagg acggcataga cgttaccgtc ctgaccaact
    13141 cgctacaggc gaccgacgtt gccgccgtcc attccggcta tgtcaaatac cgaaaaccgc
    13201 tgctcaaagc cggcatcaaa ctctacgagc tgcaacccaa ccatgccgtc cctgccacaa
    13261 aagacaaagg cctgaccggc agctccgtaa ccagcctgca tgccaaaacc ttcattgtgg
    13321 acggcaaacg catcttcatc ggctcattca acctcgaccc ccgttccgca cggctcaata
    13381 ctgaaatggg cgttgttatc gaaagcccca aaatcgcaga acagatggag cgcacccttg
    13441 ccgataccac acccaaatac gcctaccgcg ttaccctcga caggcacaac cgcctgcaat
    13501 ggcacgatcc cgccacccga aaaacctacc cgaacgaacc cgaagccaaa ctttggaaac
    13561 gcatcgccgc aaaaatccta tccctgctgc ccatagaagg tttattatag aaatgtagcg
//
LOCUS       DAWFAD010000045        13506 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWFAD010000045 DAWFAD010000000
VERSION     DAWFAD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.045"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(77..152)
                     /locus_tag="WGO49_000967"
     tRNA            complement(77..152)
                     /locus_tag="WGO49_000967"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Asn,seq:gtt)
     gene            complement(343..1095)
                     /locus_tag="WGO49_000968"
     CDS             complement(343..1095)
                     /locus_tag="WGO49_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ0306089.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSILIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            1406..1930
                     /locus_tag="WGO49_000969"
     CDS             1406..1930
                     /locus_tag="WGO49_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ0306090.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNAPPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHWVVADIPADVRRRN
                     AASLQLSRCANIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     TLCGTFSSASTAAA"
     gene            complement(2019..2298)
                     /locus_tag="WGO49_000970"
                     /pseudo
     CDS             complement(2019..2298)
                     /locus_tag="WGO49_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(2670..3815)
                     /gene="dapE"
                     /locus_tag="WGO49_000971"
     CDS             complement(2670..3815)
                     /gene="dapE"
                     /locus_tag="WGO49_000971"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ0306091.1"
                     /translation="MTETQSLELAKELISRPSVTPDDRDCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            complement(3928..4548)
                     /locus_tag="WGO49_000972"
     CDS             complement(3928..4548)
                     /locus_tag="WGO49_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEZ0306092.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYRPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYAQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPITLPDYNRWLDNFDTEKYAAFDMWHGAESEYDDHRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(4582..5091)
                     /locus_tag="WGO49_000973"
     CDS             complement(4582..5091)
                     /locus_tag="WGO49_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0306093.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDAEAFKRFVAGYDVHLAIEEPLFDMGNAFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(5186..5737)
                     /gene="azu"
                     /locus_tag="WGO49_000974"
     CDS             complement(5186..5737)
                     /gene="azu"
                     /locus_tag="WGO49_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ0306094.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     DYKFACTFPGHGALMNGKVTLVD"
     gene            complement(6018..6440)
                     /locus_tag="WGO49_000975"
     CDS             complement(6018..6440)
                     /locus_tag="WGO49_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ0306095.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACVEDGRLILLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAAKLEKRHPELAEALANLVRRHGA"
     gene            6571..9321
                     /gene="secA"
                     /locus_tag="WGO49_000976"
     CDS             6571..9321
                     /gene="secA"
                     /locus_tag="WGO49_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ0306096.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            9467..11239
                     /locus_tag="WGO49_000977"
     CDS             9467..11239
                     /locus_tag="WGO49_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0306097.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIEALKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            11427..13355
                     /gene="rpoD"
                     /locus_tag="WGO49_000978"
     CDS             11427..13355
                     /gene="rpoD"
                     /locus_tag="WGO49_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ0306098.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
BASE COUNT         3441 a         3831 c         3344 g         2890 t
ORIGIN      
        1 ctatcttgcc gcattccgaa cgccgtacgt gcaaatatga aaaagcccga acattcaagt
       61 tcgggcttca aaattctggc tccccgacct gggctcgaac cagggacctg cggattaaca
      121 gtccgtcgct ctaccgactg agctatcggg gaatggggtg tattatagcg tccgaaaaaa
      181 atgtgtcaat ccttaatttt ggaaaaatgg gcgacaaaac gacaagcata tgaatcagaa
      241 agacattaag accgatgcct taaaaggatt gccgttgtat gaatttccac agccgtcatc
      301 acaccatatt taagcccgat gagccgttct gccctccccc gcttaaaaca atgccgtctg
      361 aacttcgccg cgttccaaag ccagtaaaaa ctgtttgcgg ttcaacccgc ccgcgtagcc
      421 ggtcagtttg ccgtcgctgc cgatgacgcg gtggcaggga atcaggatag atactttgtt
      481 ctgcccgttg gcggcggcaa cggcgcggac ggctttgggg ttgcccaaac gctgcgcctg
      541 ctccttgtag ctgcgcgttt cgccgtaagg aatcgccaag agcgcgtccc atgcctgctt
      601 ttgaaactcg gtgccaatct gctccaaagg cgtggcaaag gttttcagac gacccttgaa
      661 gtataagtcc aattcctgcc gcaaaagttg cgtccgctca tcctcccgaa acacaaaccg
      721 tccgcgcaag gctttttgga cggcggcaat ttcctgttcc aaatgcttct gtccgacaaa
      781 ctccagcaga cacaatccct tatttccgaa caccgccagc atctcgccca aaggcgtggc
      841 aatggcggca cacaccagct cgttcaagct gtcgggataa cgcacttcca acagacggat
      901 ggctcggcgg atacggacat attcttcagg cgcgcagccg atattgtccc aaaaatcccg
      961 atcgaactgc ccggcttcgc attccgtcag attgggatgc ggcataacgc cgcactcaaa
     1021 aacccgggtt tcgaggcaat ggcggatttc atcccatttt gacggcagat tgtttaagga
     1081 aggcagggta atcattcgtt tgctccgtat ctctatcatg gatttggcgg caaaatcccc
     1141 aattctcgtc attcccgcac gccgatgcag gaacgggcta tgacgtaaat cttgagggtt
     1201 gggttgcggc aatgcctaaa tatgcgatat ttctaaagcc tcagggaaag gaattaaaga
     1261 cttcagacga cctgcaaagg gtcgtctgaa accacgattt ttgcatttac gcattccggc
     1321 acatcatcca accgtttcgg cacattcctg ccgccgttga cagcctataa tgaatccact
     1381 tattcatcaa gcaaaggaat catctatgca aacccgcatc ctctccgccg tactgctggc
     1441 tttttcaacc gctgcctttg ccgggggcgc attcacgctg caattcgaca acccgtccga
     1501 agacggcggc ttcacgcaaa accagctttt gagtgcgcct tacggcttcg gctgttcggg
     1561 cggcaatgct ccgcccgcgc tgtcgtggaa aaatccgccc gccgggacaa aaagtttcgt
     1621 cctgaccgtt tacgataaag acgcgccgac cggactgggc tggatgcact gggtggtcgc
     1681 cgacattccc gccgatgtcc gccgccgcaa cgcggcctcg ctgcaattaa gccgctgcgc
     1741 caacatcgcc gacgaccagt ccgcagccat atcggcggta atcagtttgc ggatttgccg
     1801 catcaggttg acgccttcgt acacggcaaa accgatgccg tcatgctgca accacgccaa
     1861 cacgccgcaa agcgcggcct ccgccacatt gtgcggcact ttttcatccg ccagcaccgc
     1921 agccgcataa tcaaacgccg cgcccatccg cccgggatgc ggcagcttca ccgcatcgca
     1981 cactgcctgc gccgtcccgt attgtgcggc aaacctttct atggcttcct gttcgaaagc
     2041 aatccattgc gcctgataga ggtcgtctga atcgggaata ttgatgacgt caaacgtctg
     2101 cccgcctgcc aaggcgaccg cctcacccgc cgcaatcctc aattcgcgcc tcgctcaccg
     2161 cagcttcttg cttccgcgcc gcacgataag cacagccggt tcatataccg ccacgctgcg
     2221 gtacaaggcg gtatgatgtt gcacgatgcc gcccaaagca cccaatcgtt cgcgcgtatg
     2281 aaagtaattt gacggcacta ttatctccaa cacgcagggc cgcacataag gcgcagccct
     2341 gtttaccctg atttggaagg ggttacttcc caaaaataaa ccaaaatccc actgccccga
     2401 atggccgggt ttcaaccata ttccgtcggt gtgcctgcaa cttgactccc tcccctgtgg
     2461 gggagggttg gggagagggt aaaacggggc agatacagac aatatttccg ttaccgcccc
     2521 gatgccctct ccctaaccct ctcccacggg agagggaatg gattgccgtt gaaataaatc
     2581 gctctacata aaaaatcaat gtgttatctc aaacccacgt taggtctaag caaatggtcg
     2641 gacatccgta tccggcaagc aagctgcttt cagacggcat ttccagccaa caagcgcgcc
     2701 aatatcccct catacaccgc cgacagcttc ggaatatcgt ccagccgcac gttttcgttg
     2761 atttggtgga tggtcgcatt ggacgggcct aattcgatga gttcttttgc aatggctttg
     2821 atgaagcgtc cgtccgaagt gccgccggtg gtggacaatt cggcctcaat gccgcaggtt
     2881 tcggcaatgg ctgcgcgtgc cacgtcggtc agtttgcccg cttgggtcag aaagggctgc
     2941 cccgaacacg accactgtaa atcgtattgc acgccgtgtt tgtccaaaat ggcgtgaacg
     3001 cgttgtttca gccctgcttc ggtggactcg gtggagaagc ggaaattgaa tttgacgttc
     3061 agctcgcccg gaatgacgtt ggtcgcgcct gtaccgccgt tgatattgga aatttgaaag
     3121 ctggtcggcg gaaagtattc gttgccttcg tcccagactt cctgcgtcag ctctaacaag
     3181 gccggggcaa aagtgtggac gggattgatt gccaaatgcg gataggcaat atgtccttgc
     3241 ttgcctttta cggtcaggtt gcccgacagc gagccgcgcc ggccgttttt aatcatatcg
     3301 cccaatttgt ccacggctgt cggttcgccg acgatgcagt agtcgataag ctcgtcgcgc
     3361 gctttcaata catcgacgac tttggtcgtg ccgtccaacg cgtcgccctc ttcgtcggaa
     3421 gtaatcagaa gcgcaatgct gccttggtgg tcgggatgtt cggcaacgaa gcgttcacag
     3481 gcggtaacga agcaggcgat gctggttttc atgtctgccg cgccgcgccc gtataatctt
     3541 ccgtcgcgct cggccggttc gaacgggggc gaatcccatt tttcgacagg acctgtcggt
     3601 acaacgtcgg tatgccctgc aaaacagacg acgggagctt tcgtgccgcg tcgcaaccag
     3661 atgtttttgg tgtcgccgaa atggagttct tcagccgcaa aaccgatttt gtgcaggcgt
     3721 tcggcaagga gtttttggca atccctgtcg tcaggggtaa cggatggtcg ggaaatcagc
     3781 tctttggcaa gctctaggga ttgagtttcg gtcatatttg ttcacttttg aaattagacc
     3841 gtctgaaacg ttctgaatgt gattttcaga cggcatttag gttaggttgg catacggggt
     3901 gggtatttta cccatcagtc ttctgaatca tttgccgtgg caggcttcat aaagcggcag
     3961 caaatcttcc accgtttccg ctatccattt cgcgacatcc tgcctgccca aatcgtcgcg
     4021 ttcgatgtgt ttgccaatgc agaaaaagtc ttcgtcgttt tgcaactttc ggtcggactc
     4081 gttttgttgc gcgacggtgc ggtggtcgtc gtattcgctt tccgcgccgt gccacatatc
     4141 gaatgcggcg tatttttcag tgtcaaaatt atccagccat cggttgtaat cgggcagagt
     4201 aatcggcgac acgtcggctt tgtagcagtg ccaatccaag ctgacactca gacggcggcg
     4261 gttaagtagt atcgacagga tggcggcaga gtttttgtat tgggcgtatt tgaaataggc
     4321 aaagaaatgg gcgcgaacct gccagccgtt acaccagcgt tcgatgtgcg gcggcgcaaa
     4381 cggcgcaccc aattcggcgg caacctgctg aatcagctgc tgccatatct gccagttttc
     4441 cttgtagtcg gctttgattt gcggaatgct ttcaggccgg tattttttaa gctgggcaaa
     4501 ctggaaaaac gggatattga acaaatcgca acttttcggg gtcagcataa tatatccttg
     4561 agacgattgt ttcagacggc attatttgcg ccggcgcgcc gccatgattt cgccgatttc
     4621 ggtcagtttt tcttttggga taaaggcgtt gcccatatca aacagcggct cttcaatcgc
     4681 caaatgaaca tcatatcccg ccacaaaacg tttgaacgct tccgcatcgg gaacataagc
     4741 attgtctgct tcgagtttgg caaattcggc ggaaacagcc gcccagttgt cgtgcagccc
     4801 gatatgttgg cgcaaaagct cgtccacgct ttcttgggct tgcggcgcat attgcagcag
     4861 cagcgggaag aagttttctt cttcgtcttc atggtgcagc ggcgcggcaa cgttgaaata
     4921 ctgggcgatt tggcggatgg tttgcaaaac aatctgattg cagccgtttt cggcgatata
     4981 gtccgacagc atggcgactt gtccgcaaaa acggcgcact ttgccgtggc aggcatacag
     5041 catttcaatc ggttcggcaa aggtaacgct tttggtttca aacggattca tgttttcgtt
     5101 ctcaacgggc acttttcaag cagtcatttt ataataaaac agcctgcaca aagcaggctg
     5161 tccgtctttt gagactttaa gcggattaat cgaccaaagt cactttgccg ttcatcaaag
     5221 caccgtgacc cgggaaggtg caggcaaatt tgtagtcgcc gtcagccaat ttggcaggat
     5281 ccagagtcag ggaagactct tcgccgccgc cgatcagttt ggtgtgggca acaacgcgcg
     5341 catcgtcagg tttgacatag tcggtatcgg cagcacctac gccgtcttta aatacgccgt
     5401 ccatgtcttc agctttggca atcacaaggt tgtgacccat gctggctttg ggttgcgtac
     5461 cggtatgttt cagagtgatg gtaaactctt tacatgcttt gctgacttgg atgtctttgg
     5521 tgttgaactg catattgtcg ttggattcga cagttgccgc acaattgccg gcagcagggg
     5581 cttcggcagc atctgcagga gcagcttcgg cggcaggcgc ttcggaagcg ggtgcttcag
     5641 cagcaggagt tgcctcggca gcaggcgcgg caggttcttg agagcaggca gccaaaccga
     5701 taacggcggc agaaatcaga gccagataag ctttcatggt aaatctccaa tcgataaaat
     5761 aatattcggt tttacagaaa tcaaagtgca accgccatta acaaaacctt gaaaaagatt
     5821 ccgccgcgtt gcacaaacag atgtttcggg gcggcatttt gctacaaatt tcatttgaaa
     5881 tcaaagcctg tctgcaagtt tacaatcgtt tactttaaaa ggggcaattt taccccgaac
     5941 ctatttcttt agtattagac ccattatcct ttacttctta atattaacgg atgtttacac
     6001 aaattcccgt atacatttta tgcgccatgc cttctaacca agtttgccaa tgcctccgcc
     6061 aattcgggat gccgtttttc caactttgcc gccgccgaac cgaaactctc cagcgcagcc
     6121 ttactcaaat gcagggtatt ggttttcggc ggtttttccg gtttcgggac cagcctgacc
     6181 gaaacagagc gtatcgaagc gtcaagccct gccagctgcg gcaataccga cggggcaatc
     6241 atcttcaagc gcgatgccgc catattgttt gccgccaaaa gaatcagcct gccgtcttcg
     6301 acgcaggccg tctgaaaatg cgggtgcagg ttggcaggca gcagtttttt cacggcggca
     6361 tccaaccgcc gccactgtcc cgcctgtttc aaaagtccgg aaagcagcgc gtcccgcctg
     6421 cccaactgtt ccaaattcat aaaacataca cccaaaaaga ttgaaatacc gcaaacgcgc
     6481 ctttatttca gacggcatta gcactttgca caaacgcttg tgttaaaatc gcgttttcgc
     6541 ccactattat atcaggcgca ggaattattc atgctgacaa acattgccaa gaaaatcttc
     6601 ggcagccgca acgaccgctt gctgaaacaa taccgtaaat ccgttgccag aatcaacgcg
     6661 ctcgaagaac agatgcaagc cctaagcgat gctgatctgc aagccaaaac tgccgaattc
     6721 aaacaacgcc tcgccgacgg tcagactttg gacggcattt tgcccgaagc cttcgccgtc
     6781 tgccgcgaag cgtcccgccg caccctcggt atgcgccact tcgacgtgca gcttatcggc
     6841 ggtatggtgc tgcacgacgg caaaatcgcc gaaatgcgta ccggcgaagg caaaaccctg
     6901 gtcgccaccc tcgccgtcta tctcaacgcg ctggccggca aaggcgtgca cgtcgttacc
     6961 gtcaacgact acctcgcctc gcgcgacgca ggcattatgg agccgctcta caatttcctc
     7021 ggcctgaccg tcggcgtgat tatttcagat atgcaaccgt tcgaccgtca aaacgcctat
     7081 gccgccgata tcacctacgg caccaataat gaattcggct tcgactacct gcgcgacaat
     7141 atggttaccg accaatacga caaagtgcag cgcgaattga attttgccgt tgtcgatgaa
     7201 gtggattcca tcttgattga cgaagcgcgc actccgctga ttatctccgg ccaggcagac
     7261 gacaacatcc agttgtacca aatcatgaac accgtcccgc cccacctcgt ccgccaagaa
     7321 accgaagaag gcgaaggcga ctactgggta gatgaaaagg cacatcaggt catcctgagc
     7381 gaagcaggtc acgaacacgc cgagcaaatc ctgacccaaa tgggattgct ggcagaaaac
     7441 gactcgctct attccgccgc caatatcgcc ctgatgcacc accttatggc ggcattgcgc
     7501 gcgcattccc tcttccacaa agaccaacat tacgtcatcc aagacggcga aatcgtcatc
     7561 gtggacgaat tcaccggccg gctgatgtcc ggccgccgct ggtcggaggg tctgcatcaa
     7621 gccgtcgaag ccaaagaagg cgtggaaatc aaacgcgaaa accaaaccct tgcctccatc
     7681 accttccaaa actatttccg cctgtacacc aagctctccg gcatgaccgg tacggcagat
     7741 accgaggctt tcgaattcca aagcatctac aacctcgaaa ccgtcatcat cccgaccaac
     7801 cgccccgtac agcgcaaaga tttcaacgac cagattttcc gttccgccga agaaaaattc
     7861 gaagccgtcg tcaaagacat cgaggaatgc cacaaacgcg ggcagcccgt cctcgtcggc
     7921 accaccagca tcgaaaactc cgaactggtc tcccaccttc tgcaaaaagc cggactgccg
     7981 cacaacgtcc tcaacgccaa agaacacgaa cgcgaagccc tgattgtcgc ccaagccggc
     8041 aaagtcggcg cgattaccgt tgccaccaat atggcgggac gcggtacgga catcgtttta
     8101 ggcggcaacc tgaagcacca aaccgatgcc atccgtgccg acgaaacctt gagcgacgaa
     8161 gagaaacagg cacaaatcgc cgcactcgaa gacggctggc aggcagaaca cgataaagta
     8221 atggaagcag gcggcctgca catcatcggt acggaacgcc acgaaagccg ccgtatcgac
     8281 aaccaattgc gcggacgttc cggccgtcag ggcgaccccg gatccagccg tttctacctt
     8341 tcgtttgaag acccgctgct gcgcttattc gcactcgacc gcgccgccgc catcctcaac
     8401 cgtctcgccc ccgaacgcgg cgttgccatc gaacacaacc tgctgacgcg ccaaatcgaa
     8461 ggggcgcaac gcaaagtcga aggcagaaac ttcgatatgc gcaaacaggt tttggaatac
     8521 gacgacgttg ccaacgaaca gcgcaaagtc atttacagcc agcgcaacga aattctgacc
     8581 agcaaagaca tcagcgacct gatgcaggaa atccgttctg atgtcgtcag cgacctcgtg
     8641 gatacctata tgcctcccga cagcatggaa gaacaatggg acatcccgac tttggagaac
     8701 cgtctggctg ccgaattcag actgcacgaa gacatccaat cctggctgaa ggcggacaat
     8761 gcgattgacg gtcaagacat caaagaacgc ctgatcgaac gcatcgaaaa cgaatatgcc
     8821 gccaaaaccg aactggtcgg taagcaggca atggccgatt tcgagcgcaa cgtgatgttg
     8881 caggtcatcg acaaccaatg gcgcgaacac ctcgccgcta tggactacct gcgacaaggc
     8941 atacacctgc gcagctatgc ccaaaaaaat ccgaagcagg aatataaacg tgaagccttt
     9001 accatgttcc aagacctgtg gaacggcatc aaattccata ttgcctccct gcttacctcg
     9061 gttcaaatcg aacaaaaccc tgtcgcggtg gttgaagagc aacccatcgg caacatccag
     9121 tccatccatt ccgaatcgcc cgatatggaa gaacttttgg gtcagtcgca aaccgatttg
     9181 gttaccgagg cctttaatcc cgatggaacg gatttcagcc ccgaagcctt ggaagcgcgg
     9241 ggacaaatcg tccaccgcaa cgacccctgc ccctgcggca gcggtttgaa atacaaacaa
     9301 tgccacggca aactggctta agcgtttgaa cgcaaatgcc gtctgaacat ccgatttccg
     9361 gcttcagacg gcattttgcc tgaaccgcca catccgactg ccattccgaa aaatcccgat
     9421 ttcgtaccgt ccgtaccaaa aacagacatc ccgtccgccc cacatcatga ttccatccga
     9481 cttcattgac gagcttttag ccaaaaccga tattgtcgat attatcgacg agcaggttcc
     9541 gctgaaaaaa ggcggggcga actatatggc gtgttgcccg ttccacaagg aaaaaacgcc
     9601 gtcgttttcg gtcagtccaa ccaagcagtt ttaccattgt ttcagttgcg gggcgcatgg
     9661 ttcggcgatt ggttttgtga tggagcatca ggggctgtcg tttccggagg ctgtgcagtt
     9721 tcttgccgac cgtgtgggca tggttgtgcc taaagtgcgt gggcagaacg ataatcccga
     9781 agtccgtgcc gaacgtaaga aaaaacagca gacgctggaa gaaacgacgg cggcggcggc
     9841 tgatttttac gcgcaacagc taaaattcaa tccggcggca aaagcttatt tggacaagcg
     9901 cggcttgagt gcagaagtta tcgcgcatta tggtttgggc tacgcgcccg acggctggca
     9961 gcctttggcg caagtgttcc aaccgtatcc gaataccgcg ttggtggata cggggatggt
    10021 gattgacaat gaggggcggt attacgaccg cttccgccat cggattatgt tccctatccg
    10081 caatccgcgc gggcaggtca tcggtttcgg cggcagggtg ctggacgact ccaaacccaa
    10141 atatctaaac tctcccgata cacctttgtt tgacaaaggg aaaaaccttt acggcttgta
    10201 tgaggggcgt gccgctgtca aggaagcgga gcggattctg gtggtcgaag gctatatgga
    10261 cgtggtcgcg ctggcacagt tcggcgtggg ctacggtgtg gcggccttgg gcacggcaac
    10321 aacagctgaa cacgtcaaaa tcctaatgcg tcaggcagac agtatttatt tctgtttcga
    10381 cggcgacagc gcggggcgaa aagcggcttg gcgcgcgctg gaaaacgcgt tgccgcagtt
    10441 gaaagacgac aaatcgctgc attttttgtt cctgcccgag gaacacgacc ccgacagtta
    10501 catccgtgcc tacggcaaag cgcaatttga agacgcgctt ctgaatcaaa gcaagccttt
    10561 gtcggagtat ttctgggaac acctttcaga cggcattcat ctcaatacgc aggaaggcaa
    10621 ggcggaattg gtgaaaacca gttcgccgct tttggcgcag attaccgcgc cggcattggc
    10681 ttatttgtta aaacaacggc ttagcgaact ggtcggcatc gaccccgaca acctcgcgca
    10741 actgctagga caggaagcgc cgaagcggca cgtcaaacaa aaaaactaca aactgcctcc
    10801 gatttccgtc aaacagcccg tcatgctgac gctggtacag cggcaaatcc gcagcctctt
    10861 gataaatccg gattgggctg catatataga cctgcccgat tatctggcgt tggacggtga
    10921 tttcgcctgc cttgccaatc ttgccgaatc gattaaaaac catgccgccg tacccgaaac
    10981 cgctcaggtt ttagagtata tgcgcggctc gccttacgaa gaaacgataa cccgaatctt
    11041 ccattcaacg caccaatcgg aagaaatgaa cagcagcagt gaagaagatt gcgagaattt
    11101 ccaaatcggc atgaaaaaac tgctcaatga gttaaaatac agccaaatcg aagcattgaa
    11161 acaaaaaagc ctgcaatccg gcttaaatga aagcgagaaa aaacttttgc tgtcgttgct
    11221 gaccgcaaaa caaaattgac cggcggattc cgccacccgt aaacccgtta tgccgtctga
    11281 aaagcactta ccccggctgc aacaacgaca cctgcagaac acccatcccc aaaagccttc
    11341 agacggcatc agagtaccct actctgccac gccttcaggt gcgtccaaac gcaaaccgtc
    11401 ggcatcttgc caacagaaag cagacaatgt ccaaaaacca aaatcacgaa gaataccaag
    11461 acgatgcccg acccctgacg attgaggaac aacgcgcgcg cctgcgccaa ctcatcatta
    11521 tgggcaaaga acgcggctac atcacctact ccgaaatcaa tgacgcactg cccgacgata
    11581 tgtccgatgc cgatcaaatc gacaacatcg tcagtatgat ttccggtttg ggtatccaag
    11641 ttaccgaaca cgcccccgat gcggaagaca tattgttaag cgacaatgcc gccgttaccg
    11701 acgatgatgc cgtcgaagaa gccgaggccg ccctttccag cgccgactcc gagttcggca
    11761 ggacgaccga ccccgtccgt atgtatatgc gcgaaatggg acaggtcgac ctgctgaccc
    11821 gcgaagacga aatcatcatc gccaaaaaaa tcgaaaacgc cctgaaaaat atggttcagg
    11881 ccatctccgc ctgcccggga tccattgcgg aaatcttaga actcatcgaa aaaatccgca
    11941 aagacgaaat ccgcgtcgac gaagtcgtag aagccattat cgacccgaat gaagtattgc
    12001 tcaacgaatt gggcttgggg cacttggaaa ccacagcgcc cgagaaacct tccaacgaca
    12061 attcggacga aaacgaagac gacgaagaat cggaagaaga tgcggatgaa atctcggcag
    12121 ccaatctcgc cgaattgaaa caaaaagtca tcggccactt tgcccaaatc gaaaaagact
    12181 acaaaaaaat gatcggtcgt ttggaaaaac accacagccg gcacaaagac tatctcgcct
    12241 accgcgacgc gattgccaac aaactgctgg aagtccgttt cgccacccgg caaatcgaca
    12301 gcctcagcag cagcctgcgc ggaaaagtag aaaacatccg caaactcgaa cgcgaaatcc
    12361 gcgacatctg cctcgaccgc gtccatatgg agcgcgacta cttcatccaa aacttcctgc
    12421 ccgaaatcac caatctggaa tggattgaag aagaaatcgc caaaggcagg gtttggagcg
    12481 acgcgctcga ccgcttccgc cacgccatcc tcgaaaaaca aaccgagttg gcagatatgg
    12541 aaaaagaaac ccgcatttcc atcgaagagt tgaaagaaat caacaaaaat atggtgtcga
    12601 gcgaaaaaga aaccgcagcc gccaaacagg aaatgattca ggcaaacttg cgcctcgtca
    12661 tttccattgc caaaaaatac accaaccgtg gcttgcagtt ccttgatttg attcaggaag
    12721 gtaacatcgg cctgatgaaa gcggtcgaca agttcgaata ccgccggggc tacaaattct
    12781 ccacctacgc aacctggtgg atccgccagg caatcacccg ttcgattgcc gatcaggcgc
    12841 gtaccatccg cattccggta catatgattg aaaccatcaa caagatgaac cgcatctcgc
    12901 gccaacacct tcaagaaacc ggcgaagaac ccgattccgc caaacttgcc gaactgatgc
    12961 agatgcccga agacaaaatc cgcaaaatca tgaaaatcgc caaagagccg atttcgatgg
    13021 aaacccccat cggcgacgac gacgattcgc acttgggcga cttcatcgaa gatgccaaca
    13081 atgttgcgcc ggccgatgcg gcaatgtaca ccagcctgca cgaagtaacc aaagaaatcc
    13141 tcgaaagcct gacaccgcgt gaggcaaaag tcctgcgtat gcgtttcggc atcgatatga
    13201 acaccgacca cacgctggaa gaagtcggca gacagtttga cgtaacgcgc gaacgcatcc
    13261 gacaaatcga ggcaaaagca ctccgcaagc tgcggcatcc gacaagaagc gaccgtttga
    13321 gaagtttctt ggacagcgaa gacagcaagc tgtaaaccaa aaaaccgcag gtttcaaata
    13381 cctgcggttt tttcttacac aataaacaac gcttccacat atcccacact cctatcccga
    13441 gacctttgca aaatccccca aaatccccta aattcccacc aagacattta ggagcacctt
    13501 cttcca
//
LOCUS       DAWFAD010000046        13389 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWFAD010000046 DAWFAD010000000
VERSION     DAWFAD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.046"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(110..538)
                     /locus_tag="WGO49_000979"
     CDS             complement(110..538)
                     /locus_tag="WGO49_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ0306099.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="WGO49_000980"
     CDS             complement(603..2351)
                     /locus_tag="WGO49_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ0306100.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPPAGDFGARFKQNTDHIVLTHVFNSGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="WGO49_000981"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="WGO49_000981"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ0306101.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="WGO49_000982"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="WGO49_000982"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ0306102.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="WGO49_000983"
     CDS             4754..4924
                     /locus_tag="WGO49_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ0306103.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..6045
                     /gene="ccoP"
                     /locus_tag="WGO49_000984"
     CDS             4948..6045
                     /gene="ccoP"
                     /locus_tag="WGO49_000984"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ0306104.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            6085..6279
                     /locus_tag="WGO49_000985"
     CDS             6085..6279
                     /locus_tag="WGO49_000985"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0306105.1"
                     /translation="MPGIPHVGIKADWADRIKKGKDTLHKHAIEGFNTMPAKGGRGDL
                     SDDEVKAAVDYMVNQSGGKF"
     gene            6481..7890
                     /locus_tag="WGO49_000986"
     CDS             6481..7890
                     /locus_tag="WGO49_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0306106.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVNTMPRNQGWYHTMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7991..11200
                     /gene="recC"
                     /locus_tag="WGO49_000987"
     CDS             7991..11200
                     /gene="recC"
                     /locus_tag="WGO49_000987"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ0306107.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPHIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKREGAGAA"
     gene            complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WGO49_000988"
     CDS             complement(11270..12838)
                     /gene="mtrF"
                     /locus_tag="WGO49_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ0306108.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAAG
                     AYFELSVPDPRPVGAKGRADDGLIHVVSLLDADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAII
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13172..13384
                     /locus_tag="WGO49_000989"
     CDS             13172..13384
                     /locus_tag="WGO49_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306109.1"
                     /translation="MKNEIQKIMDKYNPWHEDDFESYEDIARDVSLTTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3355 a         3234 c         3550 g         3250 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gatattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg ttccggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgctgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccggcgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagtggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 ataccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggca gcgtgccagc ggttttttgc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg tacccaattc ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgtggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataacgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 cacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcaccgg gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtaggcggcc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggtc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ccggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc
     6181 aatacgatgc ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg
     6241 gttgactata tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt
     6301 atcggttatg aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc
     6361 ggttttaaac gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt
     6421 agtctttatt gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta
     6481 atggatacac aaatcaaaac tgaagccgac aatcagagca accggcgtta tctgaccgtt
     6541 tggcggtggc atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt
     6601 acgggtttgg gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat
     6661 gttgtgccgc aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc
     6721 gttaatccgg aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt
     6781 gccgtgttcc gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg
     6841 gcaaaagtgg tcaacaccat gccgcgcaat cagggttggt atcacacgat ggatgaaatc
     6901 cacagcgata tgatgctcgg ttcgacaggc gattatcttt tggaaacggc tgcatcgctg
     6961 acgattatca tgataatcag cggtttgtac ctttggtggg tgaaacggcg cggcatcaag
     7021 gcgatgctgc tgccgtcaaa aggcagggcg cgttcttggt ggcggaatct gcacggcacg
     7081 tttggaactt gggtgtcgtt gattttgctg ttgttctgcc tgtcgggtat tgcttgggcg
     7141 ggtatttggg gcggcaagtt cgtacaggct tggagtcagt tccctgccgg taaatggggt
     7201 gtcgaaccga accccgtttc agtcgtgccg acccacggcg aggtattgaa tgacggcaag
     7261 gttaaggaag tgccgtgggt tttggagctt acgcctatgc ctgtttcagg gacgactgtg
     7321 ggcaaagacg gcattaaccc tgacgagccg atgacattgg aaaccgtcga ccgctttgcg
     7381 cgggaaatcg gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta
     7441 tggactttgt cgcaggattc tatgagttac gacatgatca gcccgtttgc cgaccgcacg
     7501 gtacatatcg accagtacag cggcaaaatc cttgccgaca tccgttttga cgattacaac
     7561 ccgttcggca aatttatggc ggcaagcatt gcgctgcata tggggactct gggctggtgg
     7621 agcgtgttgg cgaacgtctt gttctgcctt gccgtcattt ttatcggtat cagcggctgc
     7681 gtgatgtggt ggaaacgccg tccgaccgga gcggtgggca tcgttccgcc ggcgcagaaa
     7741 gtcaagctgc cggtttggtg gatgatggca ttgccgctat tggcaatcgc actgctcttc
     7801 ccgaccgcac tgcttgccat tgccgtgatt tggctgttgg atacgctgct gttgtcgcgg
     7861 attcctgttt tgaggagatg gtttaaatga ccaaatgccg tctgaaaggt tcagacggca
     7921 ttttgtttga aggcggacgg gggaaaggct atataatccc gaatactttt ccgcaacttt
     7981 tgtcgaaatc atgttttatc tgtatcaatc caaccgtctt gaaacgctgg cggcattgtt
     8041 tgcccgcatt caaaaagtca aaccgctgaa atcggcttta cagcccgaac agattattgt
     8101 gcagagtcag gggatgcgcc gctatctcaa tacctgcctc gcccgcgatt tgggcgtggc
     8161 ggcgaatttg tcgttcagcc tgcccgccgg cctgacgtgg aagctgatga aaaaactgat
     8221 tcccggtatt ccggaactca gcccgtttgc acccgaagtc atgcgctggc ggctgctgga
     8281 tttgttccgc agtgaagcat tccggaatac ggcagaattt gaagatgtga ggaatgtgct
     8341 gcaagactat ctgggcagcg gcgaatcggc agattaccag cttgcgggac agcttgcgga
     8401 catattcgac caatacctcg tgtaccgtcc tcagtggata gacgcttggc agcagggcag
     8461 gcggctcggt ttgggcgacg acgaaatctg gcagtccaaa ctgtggcgtt acctcgacga
     8521 tggcaggcag agcgcgccgc accgtgtcgc gttgtgggaa aagctgcttg ccgcgttgga
     8581 caaggataag ctgcccgagc gttatttcgt gttcggcatt tccacgatgg cgccgatgta
     8641 tcttcagctt ttgcacaagc tgtccgaaca ttgcgatgtg ttcgtgttcg cactcaatcc
     8701 gagcgggatg tattggggca acgtcatcga agcggcgcaa atcctcaaag gcggcggcga
     8761 tcccgattta actcaggcag ggcatccgct gctcgcctca ttgggcaagc aggggcgcga
     8821 ctttttcgac tttttgaacg aaatggaaat agaaggagaa acgccggtat ttgaagaagg
     8881 cgggcgcgat acgcttttgc acgccctgca aaccgatatt caaaacctga aaatgccgtc
     8941 tgaaaatgtg ggaagcgtca acacgggcga cggctcgata cgcatcgtct ccgcacacag
     9001 ccccttgcgc gaattgcaga tactcaaaga caagctgttg aaaattctgc atgaacatcc
     9061 cgattggcag ccgcatgata tcgccgtatt aaccccgcac atcgaatcct atacgccttt
     9121 tatcgaagcc gtgttcggac aggcgcagcc cggtgcgcag gcattgccgt attccgtttc
     9181 agacgtgaaa atcagccgcc gacaaccgct gtttcatgct ttgtcatgcc tgttcgactt
     9241 gttggaaagc cgatttgaag tcgataaagt gcttgtgctt ttagaaaccg cacccgtgtt
     9301 gcgccgtttc ggactgactg aggacgattt gccgcttttg cacgatatgg ttgccgattt
     9361 gaacgttcac tggggtttgg acggagaaat gcgcggcggc acggatcagc tgtttacctg
     9421 gaagcaggcg gtagaacgca tgatattggg ctggatgctg cccgaaggcg gcaacccgat
     9481 gtggcaggat gtcagcgcgt ggtatgccga cgtgaaccaa accgccctgt tcggacgttt
     9541 tgccgccctc ctcgaaaccc tgtcggatat tgtacggata tggcggcagc ccgcaacgat
     9601 cggcgaatgg gtggcgcgtt gccgggattt gcttgaaaca ttgttccaag ctgaagccga
     9661 tgaccaaaag tcagtccaaa accttgaaaa cgaatgggtc aaatggcagg cggaaacaga
     9721 attggcgcaa ttttccggac agttgccgca acaaaccgtc atccgccata tccgacgttt
     9781 cctcgacagc gaaagcgagg caggcttttt acgcggcggc atcacctttt gcagtatggt
     9841 gccgatgcgg agcctgccgt tcaaagtcat ctgcctgttg ggtttgaacg acggagattt
     9901 tccccgcaat accaaagccg ccgtattcga cctgattgcc aaacatcccg ccaaaggcga
     9961 ccgcgcccgc cgcgatgacg accgctacct cttcctcgaa gccctcatca gcgcgcgcga
    10021 aatcctctac ctgtcctaca tcgggcgcga catccgcaaa gacgaagagc ttgctccgtc
    10081 ttcgctgttg ggcgaactca tcgacacagt tgccgctatg gcgggcatcg gtagccgcca
    10141 acttgcacaa aactggatag aacagcatcc gctgcaagcc ttctcgcgcc gatattttca
    10201 agaaggcgga cgttcagacg gcatattcgg cacgcgtacc gactacgcca ctgcgctcgg
    10261 acaaacgccc gaaccgccgc aacccttttt cgaccaaccc gtagaaaacg ccgaacctgt
    10321 tgccgaaatc ggacaggacg aatttatccg tttctggcgc aaccccgtca aagtatggct
    10381 tcagcagcag cttgcgtgga gcgaacccca tatcggcgaa gcctgggagc ctgccgaacc
    10441 cttcgagccg caacacgccg atcaaatcgc cgaaacctac atcgaagcac ggcgcgaagg
    10501 acgggatttt gcccaaaccg ccgcccgcat cggggcggaa agcctcctgc cgtcgggaga
    10561 gttgggcaga ctttggcagc agaacttcca aactgctgcc aaacaaatcg acacggcggt
    10621 tttaaacagc cccaaactgc caccgctttc atatgccata ccgtcggacg ggcaaatcct
    10681 gaaaggcagc ttgggcaatc tgtaccgctg cggacaagtg ttttacgcct acggcaaacc
    10741 caacgcgccg caacgtatcg cttttctgct ggaacacctg atattttgcg ccgttatgcc
    10801 gtctgaagcc gaaacgcggc aaacctttat cgtccaatcc ggagaaaccg aaatattggc
    10861 ggaaatcgcg caagacaggg cattgcagct attgtcggaa tggatggcgt ttttcaatat
    10921 cgggcaaaac cgcccgctgc cgttttttgc caagacctcg cttgccgctg ccgaagcgtt
    10981 tgcccaaaaa caagattggg aagccgccct gaaaaaagcc caaaccgcct attacggcaa
    11041 caaagtcagc aaagggcaga aagactatac cgaagtcgcc ctcgtgttcg gcaacgcaag
    11101 ccaaaaccca ctcgaacaac ccctgtttga aaacctcgcc cgcctgcttg ccgacacgct
    11161 tgccgcagcg gaaaaaaggg aaggggccgg agcagcctga cgggatggaa aggcaaatgc
    11221 cgtctgaaaa cagcaaaaag gttttcagac ggcattttgt ttatcgtgtt taaggtgcgg
    11281 gatagaatgt gggcgcgccg ggaccgacgg gcaggcccaa aacaaatacc caaatgcaga
    11341 ataaggcaat ccacgcaatc aagaagaaag cggaatacgg caacatcata gaaatcagcg
    11401 tacccacgcc cgcatctttt ttgtatttga tcaccgtcgc cataatcagc ccgaaataac
    11461 tcatcatcgg cgtaataata ttggtaacgg aatcaccgat gcggtaagcg gcttgaatga
    11521 cttcgggcgc gtagccggcc aacatcagca tagggacgaa aatcggcgca gttaccgccc
    11581 attgcgcgga ggcggagcct atcatcagat tgataaaagc acaaattaaa ataaaaccga
    11641 taaacaacac gctgccgccc aagccgactt cttttaagaa cgtcgcccct ttaacggcaa
    11701 tatattgccc aatattcgtc caattaaaaa atgcgacaaa ctgtgcggca aaaaagatga
    11761 tgaccaaata aagccccaga gtactcatcg attcggccat cgcattaacg acttcctgtt
    11821 cgccgcgcaa acttcgggtt acccggccat aaacaatgcc cggcagtgca aacaacaaga
    11881 aaataaaaac aacaattgat tttaaaaacg gcgaaccgga aaccaatcct gtttcaggat
    11941 gacgcaaaat accgtcggca gggacgatgc tccaagccaa tagggcggat aaggcaacaa
    12001 acaccacgcc agcccaaatt aatcctttat attccaaagg cgtgatttca ttggaatgtc
    12061 gaatgtcttt ttcttcttgt gacaaatctg attgataagg gcccaattgc ggttcgacga
    12121 ttttttcagt aacaaaataa ccaatcaaag caatcacaaa cgtactggct accataaaaa
    12181 accagttggc ttcagggcct acgacgtagt cgggatggat gatttgcgcc gcctgttggg
    12241 tgatgcctgc caagagcgga tcgattgtgc ctaagaacag attggccgaa taaccgcccg
    12301 aaacgccggc gaaagccgca gccagaccgg caagcggatg gcggccgagg gaatgaaaga
    12361 tgatggcgga caaagggatt aggacgacat agcccaattc agaagcggta ttagataaaa
    12421 tccctgtaaa aacaaccata aaagtagtga gtttgcgtgg agattttgtg agcaataagc
    12481 gcattaatgc ggaaatcaag cccgattttt ccgcaatccc cacgcccaat aaagaaacca
    12541 acaccgttcc caacggcgcg aaaccggtga aatttttaac ggtatgcgtc aggattttga
    12601 tcaaaccgtc agcatcgagc aggctgacaa cgtgaatcaa accgtcatcg gcacgtcctt
    12661 tcgcaccaac agggcgcgga tcggggacgg atagttcgaa atacgcaccg gcggcagagg
    12721 caatcagcaa taacacaatg aaaataataa aaagcgtaac cgggtgcggc aacatattgc
    12781 ccagccattc gactgtgcgt aaaaatcgtc cgtcccgttg cgtatcggtt tgactcatct
    12841 gcttctcctt aaaaaaaata tattgagtgc attaaatttt tgaatgtaac atgaagtgtt
    12901 ttgtaagcaa actaaaataa tcgtatatgt atataaccgt aatagattaa aacagaaaat
    12961 gcccacatcg ttattctcat aaaggcggta atccgaaaac ttgacgttaa agccttatca
    13021 ggaatgactg gaactcaaaa aaacggattc ccgcctgcgc gggaatgacg ggattttcgg
    13081 tttttgtttg caagaatgcc ggagaagaat gcagtttaca ttttgaatgg tattgttatt
    13141 aattgtaaat aaatattgga aggtaaatga tatgaaaaat gaaattcaaa aaatcatgga
    13201 caaatataat ccatggcatg aagatgattt tgagtcgtat gaagacattg ccagagatgt
    13261 atcgctgacg acagataaaa cgttcattga acattatttg ttagaagttt attcagaaga
    13321 aaacggacat tttgaccaag agaatgtcca tgcaatgata gaagaaatta aaaatgcaat
    13381 ttagtgaat
//
LOCUS       DAWFAD010000047        13240 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWFAD010000047 DAWFAD010000000
VERSION     DAWFAD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13240
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.047"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(44..811)
                     /locus_tag="WGO49_000990"
     CDS             complement(44..811)
                     /locus_tag="WGO49_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0306110.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGALTALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVYETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(899..1726)
                     /gene="mutM"
                     /locus_tag="WGO49_000991"
     CDS             complement(899..1726)
                     /gene="mutM"
                     /locus_tag="WGO49_000991"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ0306111.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLIVRFQTGILLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1778..2443)
                     /locus_tag="WGO49_000992"
     CDS             complement(1778..2443)
                     /locus_tag="WGO49_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEZ0306112.1"
                     /translation="MDSWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGPWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRQLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     IAD"
     gene            2622..4598
                     /locus_tag="WGO49_000993"
     CDS             2622..4598
                     /locus_tag="WGO49_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0306113.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4666..5358
                     /locus_tag="WGO49_000994"
     CDS             4666..5358
                     /locus_tag="WGO49_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ0306114.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLENPTTLLLTITEGKYHQVKRMIAAVGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKF"
     gene            5901..6344
                     /locus_tag="WGO49_000995"
     CDS             5901..6344
                     /locus_tag="WGO49_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0306115.1"
                     /translation="MTVAGATVGFGATWRFPYLVGENGGGAYVFLFCIAMLVIGIPMI
                     LVENVIGRRKGVNALDAFGGPMNGKPIAKIWKLVGWMGLLGAFGIMAYYMALGGWVIS
                     YIVNIIGGNLNISSPIDGVVTKGFFAEHIETALGKLRFIRCFLSP"
     gene            6404..7213
                     /locus_tag="WGO49_000996"
     CDS             6404..7213
                     /locus_tag="WGO49_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0306116.1"
                     /translation="MPLLFLFLIAMVVRNVTLPGAMEGVAFYLKPDFSKITAELFVFV
                     LGQVFFALSLGFGVMITLSSYLDKNENLVQTAVITAITNTIIAILAGFMIFPSLFSFG
                     VAPDSGPTLVFQSLPIVFSHMWAGSVFAVIFFSLLLIAALTTSLTIYEVLITTIQEKT
                     KIRRTAAITIVLAAIFIFGNIPSILSYGPWKDVSIFGKNIFDAFDYISGNILFMLTAL
                     GSALFVGFVMKDEAKEELLYKGNHTTVNIWFAYVKYLVPLVILLIFISNLF"
     gene            7457..8113
                     /locus_tag="WGO49_000997"
     CDS             7457..8113
                     /locus_tag="WGO49_000997"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ0306117.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVGWHDEENVSELAKKLPAVFSGSRILLGGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYREV
                     "
     gene            8269..9954
                     /gene="rpsA"
                     /locus_tag="WGO49_000998"
     CDS             8269..9954
                     /gene="rpsA"
                     /locus_tag="WGO49_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ0306118.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGDILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            9965..10279
                     /locus_tag="WGO49_000999"
     CDS             9965..10279
                     /locus_tag="WGO49_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ0306119.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(10629..11036)
                     /locus_tag="WGO49_001000"
     CDS             complement(10629..11036)
                     /locus_tag="WGO49_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ0306120.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            11184..12293
                     /locus_tag="WGO49_001001"
     CDS             11184..12293
                     /locus_tag="WGO49_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ0306121.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
     gene            12302..13129
                     /gene="fghA"
                     /locus_tag="WGO49_001002"
     CDS             12302..13129
                     /gene="fghA"
                     /locus_tag="WGO49_001002"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ0306122.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDSSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNQE
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
BASE COUNT         3510 a         3633 c         3047 g         3050 t
ORIGIN      
        1 atccgcaaaa gcgaatcata ctgccacatt ccggcaaccg gtttcagaca gcgatatccg
       61 catcgtcggc gacaacggca cggatgcttt cttcgatttt atcttttaaa gcataacggt
      121 cttcgctttc cgccgcatcc gccacgcaaa cgaaatcgac ttttatcgtt aattttttca
      181 tagacacaat gcgccacagg caggtcggca aaccgacatc ggcatatgag ggacgagccg
      241 tcctttttcc cgtttcgtca taataacgca gcgcgaccgc caaaaccttt gcccccgcat
      301 caatggcgga ttggaacagc gcggctttga acggcaggag ccccagtccg gaggaagtcc
      361 gcgcttcggg gaaaaaactg acgttttgac cgcgttgcaa ggtttcgtag acggcgcggt
      421 taatcggttc gatgtcgcgc cgcgaattgc ggttgatgaa caccgttccc gcgttctgcc
      481 ccatcttgcc caataccggc cagcttttga tttcctgctt ggcgataaag ctgctcggat
      541 aaaccgcgct catcgcgaaa atatccagcc aggacacgtg gttggcggca accaagacac
      601 cgttcggatg ttcgggtgcg ggcctgccca cctccaatcc gatatccaaa gccgtcagag
      661 cccccctgcc caactcgatt accgcccgat tgcgcgactc ggggcagccg ccgtcaatac
      721 cgcgcaggtt tttgccggtt ttgaatagcc agaccgtcaa gcggcacaag cggcgcagac
      781 gtgtaaaaaa tgaagcttta tttgaagaca tgaacaattc tttcccgata atcggtgttt
      841 gttgttgaaa cgttcggacg gcataccgat aaaatgccgt ctgaaaccgt tttccgccct
      901 atttctgaca attcgggcag taaaacgtgc cgcgctgccc caaagtttct ttcacaacca
      961 aaccgccgca ctgggggcac ggctgattgt gccgcccgta caccgtatat tcctgttgga
     1021 agtagccact tttgccgtcg ctgtccacaa aatccctcaa ggtactgccg cccgtttcaa
     1081 tggcgcgccg caacaccgct ttgacggttt caaccaaaag cgcacactct ttctttttca
     1141 gacggttggc aggacggtgg ggcgaaatgc ccgctctgaa caggctctcg ttggcataaa
     1201 tgttgcccac accgaccacg accgcattgt ccatcagggc aagtttgacc gcgcgtttct
     1261 gcgccttcag cctcacatac agataatccg cacaaaatgc ctccgacaaa ggctccggcc
     1321 ccagtttttc caacagcgga tgatgttctt cgattccctc ataccaaagt atcgcgccga
     1381 actttctcgg atcgcggtaa cgcatgaccg tgccgtctga aaacacaata tcgacgtgat
     1441 cgtgtctgtc cggcctgccg atacgtccgt ccgacggcgt aaaaatccgc aagctgcccg
     1501 acatccccaa gtgaatcagc aaaatccccg tttggaaacg gacaatcaga tatttggcac
     1561 gcctgccgca ggacaaaacc tgccgaccgg acaaaatctc ccccaaatcg ggattgatct
     1621 gccagcgcag cttcaattgg cgcaatacca cggcttccac cgttttccct tcaatatgcg
     1681 gcgcgatgcc gcgcaacgtc gtttccactt ccggcaattc cggcataacc cctcccgaca
     1741 tttcttctga cagatgccgt ctgaaagacg gctgtcccta atccgcaatc cttgccgcac
     1801 ccgccgcaag ggctttgccg cccaaatacc cgtcccacgc gggcaggggc gttacgcccg
     1861 ccgccctttt gaccaaaacc aaaccgtaca ctgcggcaca ctgcggccac caacggtcgc
     1921 ccgccttttc cataaaccgc caaaagcgta tttgcccgag cgacgaaacg ggcggcagat
     1981 acaccataaa tttcccaaat tcaatatcga aaccgacatc cgcaagctgc cttttcaact
     2041 cgggcagcgg cagacagaag cgtttttccg gcaggcgttc gccgtcaaac caacggctga
     2101 atccccagag cgaatacgga ttgaaacccg tcagcatcaa acgtccggac ggtttcaata
     2161 tccggtgcgc ctcggacagg atttgcgaag gaacaccgcc ttcaagcgta tgcggaaaaa
     2221 gcagcatatc cgcagaaaca tccgccaaag ccatattttc cgccgacatc gacatatctc
     2281 gcggcacaca gacaacatct tcagacaggc tcagccacgg accgcccacc tgaaccgcac
     2341 acattcccga aaaacggtat gaatctagat accgcccgaa gaaatcctgt tccaattttg
     2401 caacataccg ccccatcgcc gtatcttcaa accatgaatc catattgcgt ccgtttcaaa
     2461 cagattgcct gccgatattc tattccaaac aggatttctg tcaaaaaaca catcggccgc
     2521 ccatttccga atccgcataa agttcctgta aaacttgacg ctttttcagt caaacagtac
     2581 catcggacga taaaatatgt ttattccgcc gaatataaat catgtccaaa ctcaaaacca
     2641 tcgctctgac cgcatcaggt ctgtccgttt gtccgggttt cctatacgcc caaaacacct
     2701 catcacacca aatcggtttg gcgattatgc gcttaaactc ttcaatactc gacctgcccc
     2761 cgacaaaaca atatttccaa tccggcagcc tgtggagcga gctgcgccaa ggcttccgga
     2821 tgggcgaagt caatcccgaa ctggtacgcc gccacgaaag caaattcatc gcaagccaca
     2881 gctatttcaa cagggtcatc aaccggagta gaccctatat gtaccatatc gccaacgaag
     2941 tcaaaaaacg caatatgccc gccgaagccg ccctgcttcc cttcatcgaa agcgcgttcg
     3001 tcaccaaagc caaatcacac gtcggcgcat caggattatg gcagtttatg cccgctaccg
     3061 gcaggcatta cggcctggaa aaaacaccgg tttacgacgg caggcacgac gtttacgccg
     3121 ccaccgatgc cgcactcaac tatctgcaat acctctatgg actgttcggc gactggccgc
     3181 ttgcctttgc cgcctacaac tggggtgaag gcaacgtcgg acgcgccatc aaccgcgccc
     3241 gcgcccaagg gctcgaaccg acctacgaaa acctgcgtat gcccaacgaa acgcgcaact
     3301 atgtccccaa gctgctcgcc gtgcgcaaca ttattgccac tccccaatct ttcggcatga
     3361 atatcagcga catagacaac aaaccgtatt ttcaggcagt cgaaccggat cgtccgctcg
     3421 acaacgaagc catcgcccgg cttgccggca tcacgcaaag cgagctgctc gccctaaacc
     3481 ccgcattcaa cgtccccgcg tttatcccca aaagcaaacg caaactgctg cttcctgtcg
     3541 cgtccgtaca aaccttccaa agcaactacc tcaacgccgc acccgacagc ctgttttcat
     3601 gggaagtcta tacgcctgcc gccaaaacca gcctgtccga catctcgacg gcaaccggca
     3661 tgagcattgc cgacatcaaa cgcctcaaca acctgaacgg caaccttgtc aacgcaggac
     3721 gcagcatcct tgtcgccaag aacggcaaaa cccttcagac ggcatcggaa tccgtcgttt
     3781 ccatcgacat cgacaatacg cccgacacct accgttccaa tatgccggca ggcacggtga
     3841 acgtcggcat tgcccgaatc cgacccgccg ccgcacagac agcggacatt accgtcgcac
     3901 ctttgccgca gaaaaccgtc cgtacggaac ccgatcccct tgtccgtatt gccgaacctg
     3961 cccttgcgac agccgcagcg caacctcaaa ccgaaaaaca gaccgccatg ccgtctgaaa
     4021 cccaaaccgc aacactcgcg cagatcatcc cccaaaacga catgcaggcg gcagatgaac
     4081 tcatgcagct tgttgcccga aacaacctgc gccgacaggc tgaagaaacc atctccgccg
     4141 tcatcggcac gcctgacaca gttgccgaac acaacatttc ctcatctccg caacataccg
     4201 ttgccgccga cggcaaacgc cgggcacgtt tggaaacgcg cgtagccaaa gctgccgacg
     4261 gcgaagccga aatctccccg ctccatgcca gcatccaccg cgttgtagaa ggcgacaccc
     4321 tgttcaacat tgccaaacgc tacaacgtca gcgtagccga cctgattgtc gccaacaaca
     4381 tcaaaggcaa caccatccaa aaaggacagg tactccgcct ggcgcaggca gcccctgccc
     4441 aaacccgtat tgaaaaagta tcctacaccg cgcgcaaagg cgacaccttc aaaagtatcg
     4501 ccgcgcgctt caatatccat atcgacgaca tccgccggct caatcccaac ctgaacacca
     4561 tcaatccggg acagagggtc aaactgattg gaagctgatt cggatacggc acatgacagg
     4621 actttctcag tcctgtcttt ttcatcccac atttcccata ccatcatgaa actcatcaaa
     4681 tacctgcaat atcaaggcat aggaagccgc aagcagtgcc aatggctgat tgccggcggt
     4741 tatgttttca tcaacggaac ctgcatggac gacaccgatg cagacatcga ttcctcatct
     4801 gtcgaaacgt tggatattga cggggaagca gtaaccgtcg ttcccgaacc ctatttctac
     4861 atcatgctca acaagcctga agattacgaa acttcgcaca aacccaagca ctaccgcagc
     4921 gtattcagcc tgttccccga caatatgcgg aacatcgata tgcaggcggt cggcaggctg
     4981 gatgcggata cgaccggcgt attgctgatt accaacgacg gcaaactgaa ccacaacctg
     5041 acttcgccga gcagaaaaat tcccaagcta tacgaagtaa cgctcaaaca ccccacagga
     5101 gaaacgctct gcgaaacctt gaaaaacggc gtgctgctcc acgacgaaaa cgaaaccgtt
     5161 tgtgccgccg atgccgtttt ggaaaacccg accaccctgc tgctgaccat taccgaagga
     5221 aaataccacc aagtcaaacg catgatcgcc gccgtcggca accgcgtgca acaccttcat
     5281 cgtcggcgat tcgcacattt ggaaacagaa aacctgaaac ccggcgaatg gaaatttatc
     5341 gattgtccaa aattctgaaa caaaattcca taatttccct atattcaact atatgcttat
     5401 taaataagta gttttacaaa aattaactca aaaaaaacag ttagcttgcg atataattcg
     5461 cttgtctgag taacgcctgt tcaagcaggc ttatgagtaa gacgttttcc ccgtaatgtg
     5521 ttttgccatc tatacttctc ccccttgtat agatggtttt tttatcttcg tttactacac
     5581 atactacata tgttactgac gcagcattcc cgatcatatg acatatgaaa ttttatttca
     5641 ccgtcccagt gtagaaaaat aaacgattca tctatctata aaatctgatt caatccttgt
     5701 tgatttatgc aaatcagaaa tatccactat aaaatataac tctattagtg aaaataacta
     5761 cacacttaaa aaacaatcag tcagttcctg ttaaaaaacg cgtgtagaat ttccgcttta
     5821 tctcaatcgg acacggtttc aatatgtctt ccccttcaaa taccaatcgt caaacctgat
     5881 ccagccgatt aacctatatc ctgaccgttg ccggcgcgac tgtcggtttc ggcgcgacgt
     5941 ggcgtttccc gtatttggtc ggtgaaaacg gtggtggcgc gtatgtgttt ttattctgta
     6001 tcgcgatgct ggttatcggc attccgatga ttttggtgga aaatgtcatc ggacggcgca
     6061 aaggcgtgaa cgcgctggat gcgttcggcg gtccgatgaa cggcaaaccc attgctaaaa
     6121 tttggaaact ggtcggctgg atggggctgc tcggcgcgtt cggcatcatg gcttattaca
     6181 tggcactcgg cggctgggta atcagctata tcgttaatat tattggagga aatttgaata
     6241 tttccagccc catcgacggt gtggttacaa aaggcttctt tgccgaacac attgaaacag
     6301 cccttgggaa attgcgtttt atacgctgct ttttgtcgcc gtgaaccaat ggattttggt
     6361 caaaggcgtt atcggcggca ttgaaaaagc ggcaaaatag ctgatgccgc tgctgttttt
     6421 gttcctaatc gcgatggtcg tccgcaacgt tacccttccg ggcgcaatgg aaggggttgc
     6481 tttctatctg aaacctgatt tcagcaagat taccgccgaa ctgttcgtct tcgttttggg
     6541 gcaggtattt tttgccctga gcctgggttt cggcgtgatg attaccttgt ccagctattt
     6601 ggataaaaac gaaaatctag ttcagacggc agttatcacg gcaattacca ataccatcat
     6661 cgccatactt gcgggcttta tgattttccc gtcgctcttc agcttcggcg ttgcccccga
     6721 ttccggcccg actttggtgt tccaaagctt gccgattgtg ttctcacata tgtgggcggg
     6781 atctgtgttc gccgtgattt tcttctcgct gctcctgatt gccgcgttga caacttcgct
     6841 gaccatttat gaagtgttga ttacgaccat tcaggaaaaa accaaaatcc gccgtaccgc
     6901 cgcgattacg attgtattgg ctgccatctt cattttcggc aacatcccgt ccattttgag
     6961 ctacggccct tggaaagatg tttccatatt tggcaaaaat atttttgacg cttttgacta
     7021 catcagcggc aacatcttgt ttatgctgac cgcgctcggt tccgcgctgt ttgtcggttt
     7081 tgtgatgaag gacgaagcga aggaagaatt gctttataaa ggcaaccata cgacggtcaa
     7141 tatttggttt gcctatgtga aatacctcgt gccgttggtg attctgctga tttttatcag
     7201 caacctgttc taatccgcag caatcaatgc cgtctgaagg tcatacctct ttcagacggc
     7261 attgcattga tgcccgccac aatacccgca acgccggaat ccgaaccggc ttgccggctt
     7321 ctgtcggatg tttccgggca ggcggcgttc cgtctgctta gacaatcgtc ctttaaaaca
     7381 ggtagaatcc gccccaacgg gaaacacatc cttcagacgg caaaacccat accccaaacc
     7441 atcaggaatc ccccttatga acagacaaaa agtcatcgcc atcgacggcc cgggcgcatc
     7501 gggcaaaggc acggttgccg cccgcgttgc cgccgcattg ggatacgatt atctcgatac
     7561 cggcgcactc taccgcctga ctgccctata tgcacaaaaa caaggcgtgg gatggcacga
     7621 tgaagaaaac gtttccgaac tggcaaaaaa actgcccgcc gtattttcag gcagccgcat
     7681 cctgctcggc ggcgaagacg tttcagacgg catccggaca gaagccatcg gcatgggcgc
     7741 atccgcagtc gcacagttgc ctaaagtccg cgccgccctg ctgcaacgcc aacgcgattt
     7801 tctgaccgaa aaaggactgg ttgccgacgg acgggacacc ggatcggtcg tcttccccca
     7861 agccgaactt aaaatcttcc tgacggcaga atccaaaatc cgtgccgaac gccgcgccaa
     7921 acaaatcggc atcccctgcg aaggtttggc attcgagcgc atcctgtccg acatcgaagc
     7981 cagagacgag gcagaccgaa accgcaaagt tgcccccctg aaacaacagc ccgatgccct
     8041 gcttttggac acaagccgcc tgactataga agaaactgta aaaaaagtgc ttgattggta
     8101 tcgtgaagtt taaattttca ggtataatcg cacaaattac gtttcagacg gcataaaaat
     8161 cccccatatg ccgtctgaaa ccttatgtac ccgtctgccc tgccaaggac ggcagatatc
     8221 caccaaccca ccccgcaccc cttggcggtg taccgaaaag agttatatat gtctatggaa
     8281 aattttgctc agctgttgga agaaagcttt accctgcaag aaatgaaccc gggtgaggtg
     8341 attaccgctg aagtagtggc aatcgaccaa aacttcgtta ccgtaaacgc aggtctgaaa
     8401 tcagaatccc tgattgatgt agctgaattc aaaaacgctc aaggcgaaat tgaagttaaa
     8461 gtcggcgact tcgttaccgt taccatcgaa tccgtcgaaa acggcttcgg cgaaaccaaa
     8521 ctgtcccgcg aaaaagccaa acgtgcagcc gattggattg ccctggaaga agccatggaa
     8581 aacggcgaca tcctgtccgg catcatcaac ggaaaagtca aaggcggcct gaccgttatg
     8641 attagcagca tccgcgcatt cctgccgggt tctttggtcg acgtacgtcc tgtaaaagac
     8701 acttctcact tcgaaggcaa agagatcgaa ttcaaagtga tcaaactgga caaaaaacgc
     8761 aacaacgtcg ttgtttcccg ccgcgccgtt ctggaagcca ctttgggtga agaacgcaaa
     8821 gccctgctgg aaaacctgca agaaggctcc gtcatcaaag gcatcgttaa aaacattacc
     8881 gattacggtg cattcgttga cttgggcggc atcgacggtc tgttgcacat caccgatttg
     8941 gcatggcggc gcgtgaaaca cccgagtgaa gtcttggaag tcggtcagga agttgaagcc
     9001 aaagtattga aattcgacca agaaaaacaa cgcgtttcct tgggtatgaa acaactgggc
     9061 gaagatcctt ggagcggtct gacccgccgt tatcctcaag gcacccgcct gttcggcaaa
     9121 gtatccaacc tgaccgacta cggcgcattc gtcgaaatcg aacaaggcat cgaaggtttg
     9181 gtacacgtct ccgaaatgga ctggaccaac aaaaacgtac acccgagcaa agtcgtacaa
     9241 ctgggcgacg aagtcgaagt catgattttg gaaatcgacg aaggccgccg ccgtatctct
     9301 ttgggtatga aacaatgcca agccaatcct tgggaagaat ttgccgccaa ccacaacaaa
     9361 ggcgacaaaa tctccggcgc ggttaaatcc attaccgatt tcggcgtatt cgtcggcctg
     9421 cccggcggca tcgacggttt ggttcacctg tccgacctgt cctggaccga atccggcgaa
     9481 gaagccgtac gcaaatacaa aaaaggcgaa gaagtcgaag ccgtcgtatt ggcaatcgac
     9541 gtggaaaaag aacgcatctc cttgggtatc aaacaactgg aaggcgatcc gttcggcaac
     9601 ttcatcagcg tgaacgacaa aggttctttg gttaaaggtt ccgtgaaatc tgttgacgcc
     9661 aaaggtgctg ttatcgccct gtctgacgaa gtagaaggct acctgcctgc ttccgaattt
     9721 gcagccgacc gcgttgaaga tttgaccacc aaactgaaag aaggcgacga agttgaagcc
     9781 gtcatcgtta ccgttgaccg caaaaaccgc agcatcaaac tttccgttaa agccaaagat
     9841 gccaaagaaa gccgcgaagc actgaactcc gtcaatgccg ccgccaatgc gaatgccggc
     9901 accaccagct tgggcgacct gctgaaagcc aaactctccg gcgaacaaga ataaggttgc
     9961 agacatgaca aagtctgagt taatggttcg tttggcagaa gtgtttgccg ccaaaaacgg
    10021 cacgcatctt ctggcaaaag acgtagagta cagcgtaaaa gtcttggttg acaccatgac
    10081 tagatcgctt gcccgaggtc aacgcatcga aatccgcggt ttcggcagct tcgatttgaa
    10141 ccatcgtcct gcccgcatcg gtcgcaatcc caaaaccggc gagcgtgtgg aagtacctga
    10201 aaaacatgta ccccacttca agcccggtaa agaattgcgc gagcgggtcg acttggcttt
    10261 aaaagaaaat gccaattaaa ccttagcatc aaaacgccgc tgttacgcgg cgttttttct
    10321 gtggtttaac ttcatccgct gcttcaatac cttgagccaa gcaagcaacg gattagagcg
    10381 tggatttttt atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag
    10441 agaacaattc tctaaggtgc tgaagcacca agtgaatcga ttccgtacta tttgcactgt
    10501 ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatatcattg cttacaatcc
    10561 gctttttaaa caacaaattt ttgatttcta ttacgaacag gacaaaaatc ctgcttattg
    10621 cactaaaact aagccgtttc aggaatttgc ggcaaattta cggcttttac cgaacctaat
    10681 gctttcgctt tttggtaaaa cgccaatttg tattcaagca aatctaaata gcgttttaat
    10741 tcggcaattt gacacttcac attttctatt tgattttcaa acaaggaaag gcgttcttca
    10801 atggtatcgt cgccaatgac ggtacattcc gcaaagcgtt tgatgtcttt taagctcatt
    10861 cccgtatttt tcaagcattg caataagccc aaccattgca aatcgttatc ggtaaaacag
    10921 cggttaccgt attcatcacg tccgatattg ggcaacaaac cttctttgtc gtaaaaacgt
    10981 agggtgtggg cggagatgcc tattttttcg gcggctttgg cagtagtata agtcattttc
    11041 cctccttcta aacaaaaaca gtaaaaaaca cttgctttag agttaactct aaagtgtaaa
    11101 ctgttgctat gttgctcagg caaggcaact ttgtcaatga attaagagga aagacaatgg
    11161 aaatgaaaca agccgattca accatcaaat ctcgtgcggc ggtggcattc gcccctaacc
    11221 aacccttaca aattgtggaa atcgacgtag aaatgccgcg taaaggcgag gtgttaatcc
    11281 gcaataccca cactggcgtg tgccatactg atgcgtttac gttatcagga agcgatcctg
    11341 aaggcgtatt ccctgtggtg cttggacacg aaggtgcggg tgtggtcgtt gctgtgggcg
    11401 aaggtgtgtc aagcgtaaaa ccgggtgatc acgtgattcc gctttacacc gccgaatgtg
    11461 gcgaatgtga gttttgttgt tcaggtaaaa ccaacttgtg cgtctcagtg cgtgatacac
    11521 aaggtaaagg cttaatgccg gacggcacga cgcgtttttc ttatcaaggt cagccgattt
    11581 atcactatat gggctgttcg actttcagtg aatactccgt tgttgccgaa gtttcactgg
    11641 cgaaaatcaa ccctgaagcc aaccatgaac aagtatgttt gctcggctgc ggcgttacca
    11701 caggtattgg tgcggtacat aatacggcaa aagtgcaaga aggcgactct gttgccgtgt
    11761 ttggcttagg cgcgattggt ttggcggtgg tgcaaggtgc gcgtcaagcc aaagccggcc
    11821 gcattatcgc cattgatacc aatccatcaa aatttgagtt ggcaaaacag tttggtgcaa
    11881 cggattgttt gaacccgaac gattacgata aaccgatcaa agatgtgttg ttagacatta
    11941 ataaatgggg cattgaccat acctttgaat gtatcggcaa cgtaaacgta atgcgtcagg
    12001 cattagaaag tgcacatcgt ggttggggtc aatccattat catcggcgta gcaggtgcag
    12061 gacaagaaat ttcaacgcgt ccgttccagt tggtaacagg tcgtgtttgg aaaggttcag
    12121 catttggcgg tgtgaaaggt cgctctgaac tgccgaaaat ggtggaagat tcaatgaaag
    12181 gcgacattga gttagaaccg tttgtaaccc acacaatgac actcgatcaa atcaataaag
    12241 cctttgactt aatgcacgaa ggtaaatcga tccgcgccgt tattcactac taaggtatgc
    12301 gatgaaactg attgaacaac atcaaatttt tggcggttcg caacaagttt gggcgcatca
    12361 tgcccaaacg ctgcaatgcg aaatgaaatt tgccgtctat ttgccaaata atccagaaaa
    12421 tcgaccgctt ggtgtgattt attggctttc cggcttgacg tgtacggaac aaaatttcat
    12481 taccaaatca ggctttcagc gttatgcggc agaacatcaa gtaattgtgg tggcctccga
    12541 tagcagccct cgcggagagc aagtgccgaa cgatgatgct tacgatttag gacagggtgc
    12601 aggcttttat ttgaatgcga ccgaacagcc ttgggcggcg aattatcaaa tgtatgatta
    12661 cattttgaac gagctgcccc gtctgattga gaaacacttt cctaccaacg gcaaacgttc
    12721 cattatgggg cattcaatgg gcggacacgg cgcattggta ttggcgctgc ggaatcagga
    12781 acgttatcaa agtgtttctg ccttttcgcc tattttatcg ccaagcctcg tgccgtgggg
    12841 agaaaaagcc tttactgctt atttagggaa agaccgtgaa aaatggcagc aatatgatgc
    12901 gaactcactc attcaacaag gctataaagt gcaaggtatg cgcatcgatc aaggcttgga
    12961 agatgagttt ttgccgacac aattgcgtac cgaagatttt atcgaaacct gccgtgcggc
    13021 aaaccagccg gtcgatgtgc gtttccataa aggctacgat cacagctatt acttcatcgc
    13081 cagttttatt ggcgagcata ttgcttatca cgccgcgttt ttgaagtaaa ccaaagagcg
    13141 ttcagtgttc aaagcagttt tgggatagcc ggcacgaggg cggtaagaag tgccggcata
    13201 aacgtatgcc gtctgagccg gaaggagccg actctacgga
//
LOCUS       DAWFAD010000048        12880 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWFAD010000048 DAWFAD010000000
VERSION     DAWFAD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.048"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..325
                     /locus_tag="WGO49_001003"
     CDS             <1..325
                     /locus_tag="WGO49_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306123.1"
                     /translation="AEITLETPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNN
                     ADTSFPSESVGSDAPSEAKYDLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALA
                     QELGI"
     gene            complement(497..913)
                     /gene="gloA"
                     /locus_tag="WGO49_001004"
     CDS             complement(497..913)
                     /gene="gloA"
                     /locus_tag="WGO49_001004"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ0306124.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAIEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(960..2129)
                     /gene="lapB"
                     /locus_tag="WGO49_001005"
     CDS             complement(960..2129)
                     /gene="lapB"
                     /locus_tag="WGO49_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ0306125.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVVDGRPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGEMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKGEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDMNP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(2140..2454)
                     /locus_tag="WGO49_001006"
     CDS             complement(2140..2454)
                     /locus_tag="WGO49_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ0306126.1"
                     /translation="MKLIYTFIKIIILLLFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESTK
                     QP"
     gene            2764..3762
                     /gene="ilvE"
                     /locus_tag="WGO49_001007"
     CDS             2764..3762
                     /gene="ilvE"
                     /locus_tag="WGO49_001007"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ0306127.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETEAYLDALVE
                     LVKRAADEIPDAPSALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(3948..4733)
                     /gene="fabI"
                     /locus_tag="WGO49_001008"
     CDS             complement(3948..4733)
                     /gene="fabI"
                     /locus_tag="WGO49_001008"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ0306128.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(4888..5709)
                     /gene="dapD"
                     /locus_tag="WGO49_001009"
     CDS             complement(4888..5709)
                     /gene="dapD"
                     /locus_tag="WGO49_001009"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ0306129.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPTGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(5719..5949)
                     /locus_tag="WGO49_001010"
     CDS             complement(5719..5949)
                     /locus_tag="WGO49_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306130.1"
                     /translation="MPSERRRRGIKVLPPFCIDSGAEKAIPAARPIPKRQCSATACVL
                     LTNMRLRGTIAHSFQMNILPENTGKRPTKHLT"
     gene            complement(5919..7562)
                     /gene="pgi"
                     /locus_tag="WGO49_001011"
     CDS             complement(5919..7562)
                     /gene="pgi"
                     /locus_tag="WGO49_001011"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0306131.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYVDGRDVLPEIRRELNRALEFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSSVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGTIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            8072..9304
                     /gene="pilG"
                     /locus_tag="WGO49_001012"
     CDS             8072..9304
                     /gene="pilG"
                     /locus_tag="WGO49_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ0306132.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            9378..10238
                     /locus_tag="WGO49_001013"
     CDS             9378..10238
                     /locus_tag="WGO49_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ0306133.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKKHLNLPLTEKESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            10240..10872
                     /locus_tag="WGO49_001014"
     CDS             10240..10872
                     /locus_tag="WGO49_001014"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ0306134.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAVYGIVEIPLLTEKRQFVSLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLCEKTMRLHAFYSGIFASKPTQGKHND"
     gene            10865..11074
                     /locus_tag="WGO49_001015"
     CDS             10865..11074
                     /locus_tag="WGO49_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ0306135.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSSQTESLPEISEPDMAYR"
     gene            complement(11159..12835)
                     /gene="pilB"
                     /locus_tag="WGO49_001016"
     CDS             complement(11159..12835)
                     /gene="pilB"
                     /locus_tag="WGO49_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ0306136.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
BASE COUNT         2955 a         3575 c         3378 g         2972 t
ORIGIN      
        1 agcggaaatc actttggaaa cgcctgattc caacacctct gaggcagacg ctttgcccga
       61 cttcctgaaa gacggcgagg aggaaacggt agattggagc atctacctct cggaagaaaa
      121 tatcccaaat aatgcagata ccagtttccc ttcggaatct gtaggttctg acgcgccttc
      181 cgaagcgaaa tacgaccttg ccgaaatgta tctcgaaatc ggcgaccgcg atgccgctgc
      241 cgagacagtg cagaaattgc tggaagaagc ggaaggcgac gtactcaaac gtgcccaagc
      301 attggcgcag gaattgggta tttgattccc aactgccctt tcgcagatca aggatgccgt
      361 ttcagacggc atcttttttg ccttatcggt gtaacggata aagtttgaac cggcacaggc
      421 tcaaacagca ggccgacggc aacaaaatgc cgtctgaaac ccctaaaggc ttcagacggc
      481 attggcggcg gcggtatcag gcagtttgat aggcaaccga atcgtcaccg cttttctttt
      541 gaatgaactc gattttgtat ccgtcggggt cttcgacgaa ggctatcacg gttgtgccgt
      601 gtttcatcgg gccggcttcg cggacgacgt ttccgccctg ccgcttcaca cgttcgcagg
      661 cttcgtaggc atcgtccact tcaatcgcga tgtgtccgta agcgttgccc aagtcgtatc
      721 gttccgtatc ccagttgtgc gtcagttcca aaaccgtgct gtcggtttca tcgccgtaac
      781 cgacgaaggc aagggtaaat ctgccttcgg gatagtcttt tcggcggagc agtttcatgc
      841 ccaaaacgtt ttggtagaaa tcaagggatt tttcgagatt gcccacgcgg agcatagtat
      901 ggagtaagcg catttttcgt gttcctcatt ggtggtggtt aacttcgatt tagtggtggt
      961 taaacttcaa ttttattcgg ggtaaacgtc tgccatttgt tgcaggcggg acagtgccag
     1021 aaaaagactt gggatttgaa gtggcagttg cggcaacggt acatcacgct gcgctgtagc
     1081 tgccgtccga taaccgaacg catcatgtcg gcatcggctt tccaagccgg attcatatcg
     1141 ctgagtttca aaccgagcag gcggtacacg ccgttaaggt cgggcttgcg gcggacaagc
     1201 tcgacggcgg tttgcgcggc ttctttctcg cccttaagca gcagggattt ctcgtacacg
     1261 acattgatca ggtcaagttc gggaaacgtc tgcatatatc ctgtcagacg gttcaagcct
     1321 tcttcaggtt ttccctgcgc ggcataggct tcgtaaagct tctcgccgac catgctcaag
     1381 tatgcatggt tttgctgctc gatggcggca taggcttcga cggcggcagg gaaattgcct
     1441 tgtcggtgtt cgatgtcgcc caaaatcatg ttggcgcggg tgcatttttt gttggcttcg
     1501 agtgccttgc cgacattgaa acgcgcgaca tcgaaattgg acttgaacag cgcggcttgg
     1561 gcaagttcgc aataaaactg ggcaatttca aactgatagg tctgatcgtc atggctgagc
     1621 agccgggcgg tttcaaccgc tttttcccaa tccctgtcct gttggtagat attgagcagg
     1681 tgctgtctgg cttcacgcgc catttcaccg tcttgcagcc ccaaaaaaat ctgttcggca
     1741 cgatcgacca aacccgcgct ttggtagttt tgcgccaatt caaacaggac gcgcgcgcgc
     1801 ttttcgccga ccgtatcggg agaatcgagc attgtccggt gtatgttgat ggctttgtcg
     1861 ttttcgccgc gctggcggta aagtttgccg agggtgaggt tcaaatcata cgattgcggc
     1921 cggccgtcga cgacttccgc caactccctt gccgcgcgcc cgctgttgcg gtcgaccaaa
     1981 gcgtccaagc ttttataaaa tcccgaaggg atgctttttg cctgcttcaa tacggttttc
     2041 atatccacgc gggcggcaaa ccagcccatc gcgaagaaga cgggcaaaag gataatcggc
     2101 agcaggataa tccacaattc gttgtccata tcggctttct taaggctgtt tggtagattc
     2161 gggcgcattt tgcgccggtg gtgcggtcag ctccttcccc gtcaaacgcg catttttctt
     2221 tacttcggca cgcaacctgc cgttctcgcc acgtaacgac aacaaccgtc cgaacaaggc
     2281 aaacattcca aaaataatac cgactacaaa tgcgccgaac aatacgacaa tcagcggcaa
     2341 atcgaatttt tgccccggca ggtaggaaaa ggtaacggca tccgtattaa taacggcaag
     2401 cagcaggaag agcagcagga taatgatttt gatgaaggta tagataagtt tcatgtttgc
     2461 tccgtaaata aaaacgccgc ccggacagct tcgcactctg aaccaagatt gcgctagttt
     2521 aaacgatttg cacggtttcg gataggatgc ggcagcgtgt tcagacggca tacggagtat
     2581 gcgattgccg acaattttac caaatacatc gttcctttcc atttgaaaaa taacggattg
     2641 gacaccgcat cgacagaaaa acccgccgcg cacttgtcaa aaccctgttt gcaggcgtat
     2701 ctttacaatc ttcaaattca aaccgttcat tgaaacatat cagaataaga aaggctttac
     2761 atcatgagca gacccgtacc cgccgtattc ggcagcgttt ttcacagtca aatgcccgtc
     2821 ctcgcctacc gcgaaggcaa atggcagccg accgaatggc aatcttccca agacctctcc
     2881 ctcgcaccgg gcgcgcacgc cctgcactac ggcagcgaat gtttcgaggg actgaaagcc
     2941 ttccgtcagg cagacggcaa aatcgtactg ttccgcccga ccgccaacat cgcgcgtatg
     3001 cggcaaagtg cggacatttt gcacctgccc cgccccgaaa ccgaagctta tctcgatgct
     3061 ttggtcgaac tggtcaaacg tgccgccgac gaaattcccg atgcaccttc cgcgctgtac
     3121 ctgcgcccga ccttaatcgg taccgatccc gtcatcggca aggccggttc tccttccgaa
     3181 accgctctgc tttatatcct cgcctccccc gtcggcgact atttcaaagt cggctcgccc
     3241 gtcaaaattt tggtggaaac cgaacacatc cgctgcgccc cgcatatggg acgcgtcaaa
     3301 tgcggcggca actacgcttc cgccatgcac tgggtgctga aggcgaaagc cgaatacggc
     3361 gcaaatcaag tcctcttctg cccgaacggc gacgtgcagg aaaccggcgc gtccaacttt
     3421 atcctgatta acggcgatga aatcattacc aaaccgctga ccgacgagtt tttgcacggc
     3481 gtaacccgcg attccgtact gacggttgcc aaagatttgg gctataccgt cagcgaacgt
     3541 aatttcacgg ttgacgagct caaagctgcg gtagaaaacg gtgcagaagc gattctgacc
     3601 ggtacggcag ccgtcatctc gcccgttact tccttcgtca tcggcggcaa agaaatcgaa
     3661 gtgaagagcc aagaacgcgg ctatgccatc cgtaaggcga ttaccgacat ccagtatggt
     3721 ttggcggaag acaaatacgg ctggctggtt gaagtgtgct gatgctttaa ataaaaaaaa
     3781 tgccgtctga aacccgtttg gcgtttcaga cggcattttc gcgttcggac catttccgct
     3841 gcacctgcag caagtcgggc atttccctag aaatacgctt cagacggcaa accgccgccc
     3901 gaaaccgata cgcggtacgg acggcgcacg gatttgaaaa cggcggatta tccctcggtg
     3961 ctcaaggcat taatgctgta accgccgtca acgtaagtga tttcgccggt aatgccggac
     4021 gacaggtcgg acagcaggaa ggcggcggta ttgccgactt cttcgatggt aacgttgcgg
     4081 cggagcggat tgtgggcggc gacgtgtccc aagagtttgc cgaaatcggc gatgccggag
     4141 gcggcaagcg ttttgatcgg gcctgcggaa atgccgttgc agcggatgcc ttctttgccc
     4201 agacaggcgg cggtaaaacg gatgcccgcc tcaaggctgg cttttgccat acccatcacg
     4261 ttgtaattcg gaatcgcgcg caccgcgccc aagtagctca gggcaacgat ggcggagttt
     4321 ctgccgcgca tcatcggacg ggcggctttt gccaatgcgg gcaggctgta tgcggaaatt
     4381 tcgtgtgcgg tgttgaacgc ttcgcggctg atgctgtcga ggaagtcgcc gctcaaggct
     4441 tctttcggcg caaaaccgat ggaatgcacc aaaccgtcca agccgtccca atgtttgccc
     4501 aaatcggcga acacttggtt gatttcgtcg tcgctggcga catcgcagcg gaacacgagt
     4561 tcggaatcca attccgccgc cattttgcgg acgcgctctt ccagtttgtc cacaacgtag
     4621 gtaaacgcca gttccgcgcc ttgttcgcgg caggctttgg cgatgccgta agcgatggaa
     4681 cgctcggaaa tcatgccggt aatcagaatt tttttgcctt gcagaaaacc cattttgtat
     4741 ccttcaaata gtgttcggtt cgttctaaac cgttgattat agcaaattgt ccctgtttct
     4801 gtgttttcac gttgcagcat gcaaacggca atgccgtccg aagcggattt cagacggcat
     4861 cggacgtttc aaatacggtt taaggcatca gatgccgcgc aacaattcgt tcacgctggt
     4921 tttcgcacgg gtttgcgcgt ccacgcgttt gacgatgacg gcgcagtaaa ggctgtggct
     4981 gccgtctttg gaaggcatac tgccggacac gacgaccgaa cctgtcggta cgcggccttg
     5041 gtagatttcg ccggtggtgc ggtcgaagat tttggtggat tgaccgatga acacgcccat
     5101 cgaaatcacg ctgccttctt cgacaatcac gccctcaacg atttcagaac gcgcaccgat
     5161 gaagcagttg tcttcaatga tggtgggggc ggcctgcagg ggttcgagta cgccgccgat
     5221 accgacgccg ccactcaagt gcacgttttt accgatttgc gcgcaagagc cgacagtcgc
     5281 ccaagtatca accatcgcgc cttcgtcgac gtatgcgccg atgttgacgt aagacggcat
     5341 cagcacgaca tttttcgcca caaagctgcc gcgtcgggca accgcacccg gaacggcgcg
     5401 gaagcctgcg tttttgaact cgtcttcaga ccagtcggca aacttggtcg gcactttgtc
     5461 gaagtatttg ttcacgccgt cgttgaggac ttcgttgtct tggatgcgga aggacagcaa
     5521 cacggctttt ttcgcccatt cgttgacttt ccactcaccc acgcccaagc gttcggcaac
     5581 gcgcagtttg ccggaatcga gttggcggat ggtttccaac acggcttctt tgacttcggg
     5641 agtaacggtg gtcggggtga tgtccgcgcg gttttcaaag gcggtttcga tgatgttttg
     5701 caaagacata atgtttcctt atgtgagatg tttcgtggga cgcttgccgg tattttcggg
     5761 cagtatgttc atttgaaatg aatgcgctat tgtgccacgc aaacgcatat tcgtcagcaa
     5821 tacgcaggcg gttgccgaac attggcgttt cggaatcggg cgggcggcag gtattgcctt
     5881 ttccgcgccc gaatcaatac agaaaggcgg cagtactttt atgccgcgcc gccgcctttc
     5941 agacggcatt cgcggtaaaa cgccatcagc ccttcggtcg aggcatcgtg tacggacgtg
     6001 ccgccttcca gttcgccgat gatggttttt gccaactgtt tgccgtattc caccccccac
     6061 tgatcgaagg ggttgacgtt ccatatcgtg ccttggacga aggttttgtg ttcgtaagcc
     6121 gccatcagca tacccagatt gtagggcgtg aggcggtcga gcaggatgct gttactgggg
     6181 cggttgccgg ggaactcttt gtgcggcgcg aggcgttcgc gttccgcttc gggcaaatct
     6241 gccaattcgg cgcgtgcttc gtccaaggtt ttgcccttca tcagggcttc cgcttgggca
     6301 aaggcgttgg caacggtaaa acggctgcgt ccgtcctcca cgccctgcgc cgtcatcggg
     6361 acgataaagt cgcagggaat caggcgcgtg ccttgatgaa gcagttggaa ataggcgtgc
     6421 tggcagttga ccccttcacc gccgaacacg atgccgcccg ttttgcacac ggcgggactg
     6481 ccgtctgaag cgcggctttt gcccaaactc tccatatcga gctggttcag ccacaccggc
     6541 agcaggcgca ggttgtggct gtacggaacg gcggtctgcc cgtccgcgtg ttggaaattg
     6601 ttataccaca cggcaatcag tgccatcaga acgggaatgt tgcggcgcgg cggcgtgtgg
     6661 aaaaaatggc tgtccatcgc gtgcgccccc gccaacaact cgcggaaacg cgccccgccg
     6721 accgcaacca tcacgggcaa accgacggac gaccagacgg aatagcgtcc gcccacccag
     6781 tcgtacatcg caaacacgcg ttccgccgcg ataccgaaag ccgcagctgc cgcagtgtcg
     6841 gcagacaccg cgcaaaaatg gcacgccgtt tcggattccg agaaccccgc accgcgatac
     6901 cacgccttga ctgcctgtgc attgagcagg gtttccggtg ttttgaagga cttgctggca
     6961 acgcaaaaca ctgtcgtttc ggggttcaga cggcataaaa ccgcatccag gcaggcagga
     7021 tcggcgttgg cggcaaaatg gacggcgata tgccgtctga acggctcgag tgcctgaacg
     7081 cacattgccg gcccgaggtc ggatccgcct atgccgatgt ggacaaaatc cgtaatccgt
     7141 ttcccagtta tcccctgata cgaaccgtcg tccaaactgt gtgcaaactc caacgcacga
     7201 tttaactcgc ggcggatttc gggcaacacg tccctgccgt ccacataaac ggcatccgca
     7261 ccgtcgggca ggcgcaaagc cgtatgcagc gcggcacgcc cctcgctgcc gttgactttc
     7321 gcacccgtcc gcaaagcacg cattttccct tccaaatccg ccgtctccgc aagacggcag
     7381 agcagttgca gcgtatcttc gcccaaacgg tttttgctgt aatcgaacaa catcccgtcc
     7441 aaacgctcat gcatacgctc aaaacggtcg ggttcgcagg caaagcggtc gcgcaaaagg
     7501 acatgacgcg tatcctgata atggcgttcg agcgcatacc atgcacgggt aaaagcattc
     7561 atctgttttc cttgattttt cagaaccgga ttaaaatgta gcagaatgta gtttaacaaa
     7621 cggcagcggc tttggcgaat ctccggaaca ccgcccgccg cgtccaatag caccttattt
     7681 ccatatggcg aaaatatagt gaattaacaa aaatcaggac aaggcggcga gccgcagaca
     7741 gtacaaatag tatggaaccg attcacttgg tgcttcagca ccttagagaa tcgttctctc
     7801 ttgagctaag gcgagccaac gctgtaccgg tttttgttaa ttcactatac cctccggccg
     7861 acatttgacc cgcaacaata tcctgcaaga tttattgtgt acgcataaat gccggacagc
     7921 cgcctaaata aggtataatc gcatccgatt ctgtcccgtt tgccgcccgc aggcgggatg
     7981 tcggcggttt tcatcttacc gggaacacgc cggtactgtt ttttaacgat gtttcttaca
     8041 ttttattcca attactttac ggggctagaa tatggctaaa aacggaggat tttctttgtt
     8101 cgcaaagaaa gaaaaacgct ttatctttga aggcaggcat tccgcctccg acaaactggt
     8161 caacggcgaa gtatccgcgt ttaccgaaga agaggcgcgc aaaaaactgg caaaacgcgg
     8221 catccgcccg ttgcagatta cccgtgtgaa aacaagctcc aagcgcaaaa tcacacaaga
     8281 agacatcacc gttttcaccc gccagctgtc cacgatgatt aaagcgggcc tgccgctgat
     8341 gcaggcattt gaaatcgtgg cgcgcggaca cggcaacccg tctatgacgg aaatgctgat
     8401 ggaaatccga ggcgaagtgg aacagggcag ctcgttgagc cgcgcattct caaaccaccc
     8461 gaaatatttc gaccgcttct actgcaacct ggttgcggca ggcgaaacgg gcggcgtatt
     8521 ggaaagcctg ctggacaaat tggcaattta caaagaaaaa acccaggcca tccgcaaaaa
     8581 ggtaaaaacc gcactgacct atccggtatc cgtcatcgcc gtcgccatcg gtttggtatt
     8641 cgtgatgatg attttcgtac tgcccgcctt taaagaagtt tacgccaata tgggcgcgga
     8701 gcttcccgca ctgacccaaa cagtgatgga tatgtccgac tttttcgtct catacggctg
     8761 gatggtgctg atcgcactgg gctttgccat atacggcttc cttaaattga aggcgcgttc
     8821 gattaaaatc caacggcgta tggatgccat actgctgcgt atgccgattt tcggagacat
     8881 tgtccgcaaa gggacgattg cccgctgggg caggacgaca gcgacgctga ttgcggcagg
     8941 cgtgcctttg gtcgatgtat tggactccac tgccggcgcg gcgggcaatt taatctatga
     9001 agaagccacc cgggaaatcc gtacgcgcgt catccaaggt ttgtctatga cttcggggat
     9061 gcgtgcgacg gaactgttcc ccaatatgat gttgcagatg tcttccatcg gcgaggaatc
     9121 gggttctttg gacgatatgc tcaacaaagc cgccgaattt tacgaagacg aggtggacaa
     9181 tgcggtcggc aggctgtccg ctatgatgga gccgatcatt atcgtgattt taggcttggt
     9241 catcggcacg cttctggtcg ccatgtatct gccgctgttt aatttgggca acgtggtcgc
     9301 ctgatttgcc gcacagatcc ggcgcggatt cgctctgcgc cggtttgttt ttgttttgaa
     9361 tacattgaga acaaaatatg tctgatttgt ctgtattgtc gccgtttgcc gtgcctttgg
     9421 cagcggtgtt cgggctgctg gtcggcagtt tcttaaacgt cgtcatttac cgcgtgccgg
     9481 tcatgatgga acgcggctgg acggtatttg ccaaaaaaca tttaaacctg ccgctgaccg
     9541 aaaaggaaag ccgtaccttc aacctgatga aaccggattc ctgctgtccc aaatgccgcg
     9601 tgccgatacg cgcgtggcag aacatcccga ttgtcagcta cctgctcctg cgcggcaaat
     9661 gcgcttcctg ccaaaccaaa atcagcatac gttatccctt aatcgagctg ctgaccggcg
     9721 tattattcgg gctggtcgcc tggcaatacg gctggtcttg gattacattg ggcggtttga
     9781 tactgaccgc gtttctgatt tccctgacct ttatcgatgc ggacacccaa tacctgcccg
     9841 actcgatgac actgcccttg atttggctgg ggctgatatt taatttggac ggcggcttcg
     9901 tgcctttgca gtctgccgtt ttaggtgcgg ttgccggcta tggttcgata tggcttttat
     9961 gcgcggtata taaactgatt aaaggcgaaa tcggtatggg caacggagat ttcaaactga
    10021 ttgccgcatt gggcgcgtgg ctcggcatat ccgcattgcc cgtgctgatt tttgtttcct
    10081 cgctgatcgg tttggtcgcg gcaatcgtta tgcgcgtcgc caaggggcag cattttgcct
    10141 tcggccccgc actgacagtt tcgggctgga taatttttac ggcaaacgat tccgtatggc
    10201 gggcggtcaa ctggtggctg acccatccgg tgctgtaaga tgacggtatg ggtcgggctg
    10261 accggcggaa tcggcagcgg caaatcggca gccgcgcaat gttttgccga tttgggcgtg
    10321 ccgcgcatcg atgcggacgc ggcggcgcac tcgctgacgg cttcagacgg catcgccctg
    10381 ccggaaatca ggcggctgtt cggcgacacc gtttttgaca cacagggttt gttgcggcgc
    10441 gacatattgc gtaaagaagt ctttgcctcc ccatcgcgaa aagccttgct cgaatccgtg
    10501 atgttgccgc tgattttctc agaaatcaaa aaacagcaag aaacctttac cgatgcggtt
    10561 tacggcattg tcgaaattcc gctgctgacg gaaaagcgtc aatttgtcag cctgatacgg
    10621 cgtgtcctga ccataagcgc acctttggaa aaacgtatcg gcagggtgat ggcccgcagc
    10681 gggctgacgc gcggcgaggt ggcggacatc atcagccatc aggcatccga atccgaacgc
    10741 ctgctgcttg cagacgatgt gctgctcaat gacggcagcc tcaaaagcct gtgtgagaaa
    10801 acaatgcgcc tgcacgcgtt ttattcaggg attttcgcct caaaaccaac acaaggaaaa
    10861 cacaatgact gaatcgcggc aaacacgcct tcaagtcaaa tgtccgacct gtcaaacggc
    10921 agtagtatgg aaaccggaaa acgcattccg ccccttctgt tcgcaacgct gcaaactgat
    10981 cgacttgggc ggatgggcag acgggaaata tacggtttcc agccaaacgg aaagtttgcc
    11041 ggaaatatcc gaacccgaca tggcataccg ctgaccgccc cgccttcccg gcaaacaccc
    11101 tgaaagtcaa atgccgtctg aaacaaacac gcttcagacg gcattttcat tctcaaacct
    11161 aatcgttggt atttgccgtt acctcttcca atgaagtaat gccctgcata actttcaaaa
    11221 taccggcccg gcgcaaatcc accataccct ccttataggc aacgtccaaa atatccactt
    11281 ccgtaccgtt gttcataatc acacgctgca tttcttcgct gatgggcata acctcataca
    11341 cgcccgcacg ccccttataa ccctgccccc ggcaacggtc gcaaccgacg gcgcggtaaa
    11401 gtttccaatc ttttgcaagg tcctcatcgg tgaagccgac ttccttcaaa gcagaggcag
    11461 acgggcgttc cacttcctgt ttgcagctcg aacacagcct gcgtaaaaga cgctgcgcca
    11521 taatcaggct gaccgaactg gcaatattaa acggcgcgac acccatattc agcatacgcg
    11581 acaacgtcgc cggcgcatta ttggtgtgca gggtggaaaa caccatatgc cctgtttgtg
    11641 ccgccttaat cgcaatatcg gcagtttcca aatcacgaat ctcaccgacc ataatgatgt
    11701 ccgggtcctg acgcaggaaa gacttcaaag cagcggcaaa agtcaggccc tgcttatcat
    11761 tgacgttaac ctgattgatg cccggcaggt taatctcggc agggtcttcc gccgttgcaa
    11821 tatttaccga ctccgtattc aaaatattca aacaggtata gagcgacacc gtcttacccg
    11881 aacccgtcgg accggttacc agcaccatcc cgtagggacg gtgaatcgct tccaacaaca
    11941 attttttctg aaacggctca aaaccgagct ggtcgatgtt caaagacgcg gcatcggaat
    12001 tcaaaatccg catcacgacc ttttcgccaa acagcgtcgg caatgtgctg acacggaaat
    12061 cgacaggctt gccgcccttt tgaaaggtca gctgcatcct gccgtcctgc ggtatccgtt
    12121 tttcggaaat gtccaaacgc gacattacct taatccggga agcaagctgc ccccttaccg
    12181 caatgggcgg ctgaaccacc tcgcggagct gcccgtccac acggaaacgg atacgggcat
    12241 tgtgttcgta aaactcgaaa tggatgtccg atgccccgct gcgcaaggca tccgacaaag
    12301 tcttatggat aaacctcgga acagggccgt cttctgcctc ctcgttgtcg atatacaggg
    12361 tgtggctttc ctcttcctcc tgcccctccc caagctcctg aagcagcgat gtcgaacgcg
    12421 aacccaccca atcgagcaaa cccgccaact ggtcatcctc gacaatgacc aactcaacct
    12481 caatccctgc ggcagaaacg gttttctgaa tttgcggcat ctgtgtcgga tcggaaaccg
    12541 caaaaaatac tttgtcgccc cgacggaaaa ccggcacaca gtggaactcc accatctgct
    12601 cctccgtcaa cacccccatc agcaccctgt ggcgcggata atgacgcaaa tcaagaatcg
    12661 aataactgaa caccctcgca atcaatgccg caagcgactt gggcgaaatg acaccgtctg
    12721 aaaacagcat cggcaacact tccttacccg cctgcgactc attgtagtaa tgctcggcct
    12781 gctcaacagt aaccacctgg ttttgaacca gaatcctcag caaacctacg ctcatacgac
    12841 cttatcccca aatttattca ttgttatacc tgtacagctt
//
LOCUS       DAWFAD010000049        12748 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWFAD010000049 DAWFAD010000000
VERSION     DAWFAD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12748
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.049"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(142..232)
                     /locus_tag="WGO49_001017"
     tRNA            complement(142..232)
                     /locus_tag="WGO49_001017"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Ser,seq:gga)
     gene            complement(268..354)
                     /locus_tag="WGO49_001018"
     tRNA            complement(268..354)
                     /locus_tag="WGO49_001018"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(318..320),aa:Leu,seq:cag)
     gene            complement(390..476)
                     /locus_tag="WGO49_001019"
     tRNA            complement(390..476)
                     /locus_tag="WGO49_001019"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(440..442),aa:Leu,seq:cag)
     gene            565..2940
                     /gene="rnr"
                     /locus_tag="WGO49_001020"
     CDS             565..2940
                     /gene="rnr"
                     /locus_tag="WGO49_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ0306137.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQF
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAIKVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAGKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3279..4742)
                     /gene="guaB"
                     /locus_tag="WGO49_001021"
     CDS             complement(3279..4742)
                     /gene="guaB"
                     /locus_tag="WGO49_001021"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ0306138.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSADKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4889..7447)
                     /gene="glnD"
                     /locus_tag="WGO49_001022"
     CDS             complement(4889..7447)
                     /gene="glnD"
                     /locus_tag="WGO49_001022"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ0306139.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKTNRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGGIDELCESVRNDITGDTAFLEARFLFGNRQTADELAEKMNAQRNVAA
                     FIEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATNLPALVK
                     QGILTRTEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYQGLNLRR
                     QSEELMRVFYRAIKTVKQLSGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFERQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTEEESDLLAWLVENHLLMSTVAQKEDIQDPSVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLAGNGGNPHALFGRRQQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAEISSHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEIFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQALLEQLA"
     gene            complement(7496..7816)
                     /locus_tag="WGO49_001023"
     CDS             complement(7496..7816)
                     /locus_tag="WGO49_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0306140.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            complement(8140..8613)
                     /gene="bfr"
                     /locus_tag="WGO49_001024"
     CDS             complement(8140..8613)
                     /gene="bfr"
                     /locus_tag="WGO49_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0306141.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLEKEKEKHEALI
                     AAISVAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(8641..9105)
                     /gene="bfr"
                     /locus_tag="WGO49_001025"
     CDS             complement(8641..9105)
                     /gene="bfr"
                     /locus_tag="WGO49_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0306142.1"
                     /translation="MQGNQAVLDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(9568..10551)
                     /gene="lipA"
                     /locus_tag="WGO49_001026"
     CDS             complement(9568..10551)
                     /gene="lipA"
                     /locus_tag="WGO49_001026"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ0306143.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPKNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(10544..11167)
                     /gene="lipB"
                     /locus_tag="WGO49_001027"
     CDS             complement(10544..11167)
                     /gene="lipB"
                     /locus_tag="WGO49_001027"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ0306144.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNANRTASTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPIVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKGGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11169..11444)
                     /locus_tag="WGO49_001028"
     CDS             complement(11169..11444)
                     /locus_tag="WGO49_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ0306145.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHKLVKVVL"
     gene            11629..12699
                     /locus_tag="WGO49_001029"
     CDS             11629..12699
                     /locus_tag="WGO49_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ0306146.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LAVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
BASE COUNT         2717 a         3014 c         3729 g         3288 t
ORIGIN      
        1 taccgcaaaa cataccacaa aaaacggtaa cggatagttg taagcggttg atgacgatta
       61 tagacaataa cgggttttcc caatgaaatt attgtttgaa taataaaaaa tcccaaaccg
      121 taaaagtttg ggatttgtat ttggcagaga ggaagggatt cgaaccctcg atacgctatt
      181 cacgtataca cgctttccag gcgtgcgact taaaccactc atccacctct ctaatggcgg
      241 aaattatccc aatcggaata atttatttgg tgcccgggag aagactcgaa cttccacacc
      301 cgtgaggata ccagcacctg aagctggcgc gtctaccaat tccgccaccc gggcaatcta
      361 aatattgaaa taaagcaaag catttgattt ggtgcccggg agaagactcg aacttccaca
      421 cccgtgagga tactagcacc tgaagctagc gcgtctacca attccgccac ccgggctttg
      481 ctttatttgc tgttttgcgg tacagtgtcc tgccgcaaag aagtcgctat tatatagttc
      541 ataaagagaa tgtcaacagt ccaaatgaat aaaaatatta aatctttaaa tttacgggaa
      601 aaagacccgt ttttaagtcg tgaaaaacag cgttatgaac atcctttgcc cagtcgggaa
      661 tggataatcg aattgttgga gcgcaaaggt gtgccttcaa aaatcgaatc gcttgcgcgc
      721 gagctgtcga ttacggaaga cgagtatgtc tttttcgaac gccgtctgaa ggcgatggcg
      781 cgggacggtc aggttttaat caaccgccgg ggcgcggttt gcgcggcgga caaattggat
      841 ttggtcaaat gccgtgtcga ggcgcacaaa gacggtttcg gttttgccgt gccgctcacg
      901 cccgccaaag acggtgattt tgttttgtat gaacgccaga tgcgcggcat tatgcacggc
      961 gatattgtca ctgttcgtcc tgccggcatg gaccgtaggg gccgccgcga agggacagtt
     1021 ctggatattg tcgaacgcgc gcaaagcaaa gtggtcggcc gtttctatat ggataggggc
     1081 gtggcgattt tggagccgga agacaagcgt ctgaaccaaa gcatcgtatt ggaaccggac
     1141 ggcgtggcgc gtttcaaacc tgaatccggt caggtcatcg tcggcgaaat tgaggtttat
     1201 cctgagcaaa accggccggc agtggcaaaa atcatcgaag ttttgggcga ttatgccgac
     1261 agcggcatgg agattgaaat tgccgtgcgc aagcatcatt tgccgcacca attcagtgaa
     1321 gcgtgtgcca aagccgtgaa aaaaattccc gaccatgtac gcaaaagcga tttgaaaggc
     1381 cgcgtcgatt tgcgcgacct gcctttggta acgattgacg gcgaaacggc tcgagatttt
     1441 gacgatgcgg tgtttgccga aaaaatcgga cgcaattacc gtctggtcgt ggcgattgcc
     1501 gatgtcagcc attatgtccg ccccgatgac gctatcgaca cggacgctca ggaacgcagc
     1561 accagtgttt acttcccgcg ccgcgtgatt cccatgttgc cggaaaacct gtccaacggc
     1621 atctgctcgc tcaatcctaa tgtcgagcgt ttgtgtatgg tgtgcgatat ggttatcact
     1681 tacgcgggca atattaaaga ataccgcttc taccccgccg tgatgcgctc tcatgcccgc
     1741 ctgacctaca accaagtttg gaaatggctt tcaggcggca tcgggcatcc gttcaaagcc
     1801 caaatcgaca cgctttacaa actcttcaaa atccttcaga aaaagcgttt cgaacgcggg
     1861 gcggtggagt ttgacagcat cgaaacccaa atgcttttcg acgacaacgg taaaattgaa
     1921 aaaatcgtcc ccgttgtccg caacgatgcc cacaagctga ttgaagaatg tatgttggcg
     1981 gcaaacgttt gcgcagcgga ttttctgttg aaaaacaagc ataccgcatt gttccgcaac
     2041 catttggggc ccacgcccga aaaactcgcc gccttgcgcg agcagttcgg tctgttgggg
     2101 cttcaacttg gcggcggcga caacccgtcg ccgaaagact atgccgcgct tgccggacag
     2161 ttcaaaggca ggccggatgc cgaattgctg caagtcatga tgttgcgctc catgcaacag
     2221 gcggtttacg aaccgcattg cgacggacac tttggtcttg cctacgaagc atacgcccac
     2281 ttcacctcgc ccatccgccg ctatcccgac ctgaccgtac accgcgccat caaagccgtg
     2341 ttgaatcagc aaacctacac gccaaaaaaa agctggcagg ctttgggcgt gcatacctcg
     2401 ttctgtgagc gccgtgccga cgacgccagc cgcgacgtgg aaaactggct gaaaacctac
     2461 tatatgcgtg acaaagtcgg cgaaatattt gaaggcaaaa tttccggggt ggcaaatttt
     2521 ggaatatttg tcactttgga cgatatccat atcgacggtc tggtgcatat cagcgatttg
     2581 ggcgaagact atttcaactt ccgccccgaa atcatggcaa tcgaaggtga acgcagtggc
     2641 atccgtttca atatgggcga cagggttgcc attaaagtgg cccgtgccga tttggatgat
     2701 ggaaaaatcg attttgtcct gattgccgag gggagcggca gagggcggaa agttaaatca
     2761 tccgcgtctg ccaaaccggc agggacggcg gggaaaggga agccgaaaac cgccgccggg
     2821 aaaaaaacag cccgaggcgg caaagtaagg ggaaggggcg cgtctgccgc cgcagaatcg
     2881 gggaaaaagg caaagaaacc ggttccgatt aaggtaaaaa aacggaaagg caaatcataa
     2941 tgctgacggg gcggcttggg gagggagggg catactttcc cgaagccgcc gcccgatttt
     3001 ttgatatggc ggccggcgca tcatttcagg ccaatcgaaa cgcccgtatt gaaagattgc
     3061 gtttatttcc accgccgttt taaaggccag cggtattcgg cagacggggc gcaaacggcg
     3121 ttcagacggc attttcattc tttcggcgtg tccgtccgaa tggctttgcc cgtccgcgca
     3181 atcagcccgt gcggcttgcc ttgaacggac aaaaaatgcc gtctgaaacc cgaaaatcag
     3241 gtttcagacg gcattttgcc ttgaaaaggc tgttcaaatc agcggtggta gttcggtgct
     3301 tctttagtga tttgaacgtc gtgaacgtgc gattcgctca tacctgcgga agtgatttcc
     3361 acaaattcgg ctttttcgtg catttcggca atattggcgc aacccaagta acccatactg
     3421 gaacgcagac cgccggtcag ctggtggatg atgttcacaa tcgggccttt gtaaggaacg
     3481 cggccttcga tgccttcggg gacgtatttg tctgcgctgt cggttttgtc ttggaagtag
     3541 cggtcggcag aaccttggct catcgcgccc aaggaaccca taccgcgata ggatttgtac
     3601 gagcggcctt ggtagagttc gatttcgccc ggcgcttctt ccgtgcctgc aaacataccg
     3661 ccgagcatga cgctgtacgc gcctgcggcg agggctttgg cgatgtcgcc ggagaagcgg
     3721 atgccgccgt cggcaatcag cggaacgccc gtgcctttga gggcttcggc aacgttgtga
     3781 atggcggtca gttgcggcac gccgacacct gccacgatac gggtggtgca aatcgatccc
     3841 ggaccgatac cgactttgac ggcatccgcg ccggcggcga ccaaatccaa tgcggctttg
     3901 gcagtggcga tgttgccgcc gatgacttgg atgtgtggat aggtttcttt cacccatttc
     3961 acgcggtcaa tcacgccttg gctgtgcccg tgggcagtat cgacgacaat cacgtccacg
     4021 ccggcttcga ccaaggcttt gacgcgctct tcggtatcgc cgccggtgcc gactgccgca
     4081 ccgacgcgca gacggccctc ggagtctttg ttggcgttgg gaaactcggt ggtttttaaa
     4141 atatctttaa cggtaatcag acctttgagt tcgtcttttt cgttcagaac caaaacgcgc
     4201 tcgactttgt gcgtgtgcat cagttcgcgc gcttcgtcta tgcttgtgcc ttcggggacg
     4261 gtaaccagac gttcgcgcgg ggtcataatg gcggaaacgg gcaaatcgac gcggttttca
     4321 aaacgcaggt cgcggttggt tacgatgccg acgactttgc cgttttcaac gacgggcagg
     4381 ccggacattt tgcgtttgcg ctgcgcgcgc atttccaaga cttcgcggat gagcgtcgtc
     4441 ggtgcaacgg ttacggggtc tttgaccacg ccgctttcgt ggcgtttcac tttggaaacg
     4501 gcgcgcgcct gcatttcggg cggcatgttt ttatggatga tgccgatgcc gccttcctgt
     4561 gccatcgaaa tggcgaggcg cgcctcggta acagtgtcca tcgcggcgga aagcaggggg
     4621 aggttaagtg tgatttcgcg ggtgagcttg gtttgaagtt taacgtctcg cggcagcacg
     4681 gtcgaatgtg cgggaaccaa caaaacatcg tcgaaagtat aggctttttc tacgatacgc
     4741 ataatgctcg gtctttcagt ttgtgcaaga tgcacggcat tatagcacgt taccggcggc
     4801 ttgacagtct atattgtttg caatatcccc cgccttcaga cggcatacgg cgcggcggcg
     4861 gaatgccgtc tgaaaaccgg cagggtgctt atgccaactg ttccagcaaa gcctgtttca
     4921 atgaggactg gatttttggg tttttcaggt cggggctaaa aacggtaaaa ctgtcttcgg
     4981 cgcgttcatc cagtgtggag attttggcat agcgcaggct gacgttgtgg gcgaaaaaga
     5041 tttccgccat atcggcgagc aggaaggggc ggttgacggc ggtaatttcg acggagtacc
     5101 agtcggggta gtcttcttcg ggggtaatgg tgatgcttgg cgcgatcggc atgtagcggc
     5161 tgcggcggct gatgcggcgg ctatggctgg agatttcggc aacggtgtgt ccgtggataa
     5221 agctgttgag ttcggcttcg agcgcgcttt ggatgtcggg gtagtcttcg ggagcgtgct
     5281 gcgaggggat ttgcacgatg aaggtgtcga ggatgtagtc gtgttcggtg ataaaggcgc
     5341 gggcggcgag aatgtcgaag ccgtggcggc tgaagatacg gcagaggcgg gcgaacaggc
     5401 gcgggccgtt gggcataaag accatcactt ggaagctgtc gctttggggc aggatgcggc
     5461 tgcggacgat gggggtttca aaatcgtgta cgaggttggc ggcgtgccac aggatttcgc
     5521 gggactggtg gcgggcgaag taggcggaac cgagcgcgtt ccaaagcttt ttctgctgtt
     5581 tttcggggac ggcggcgcgg gtgagcaagt cggcggcttc ttgttggcgg cggccgaaga
     5641 gggcgtgcgg gttgccaccg ttgcctgcga ggtagcgtcc ggcggcatgg aagaggcttt
     5701 ccagcaggct ggcgcgccat gcgttccaca gcttgggatt ggtaccgcgt atgtcggaaa
     5761 tggtcagaag gtagagcgcg ctgaggcgtt cgtgggtttg gacgcgtttg cagaaggcat
     5821 cgagtacgct ggggtcttgg atgtcttctt tttgggcgac ggtggacata agaagatggt
     5881 tttcaaccag ccaggcgagc aggtcgcttt cttcttcggt caggaagtgg tcggcggcaa
     5941 attggcgcgc gtctgcgatg ccttgtatgg catggtcgcc gccgcgtcct ttggcgatgt
     6001 catggaaaaa ggcggcaagg tagaggatgt cttgtctttc aaaggactgc atcagtgcag
     6061 aggcgtaggg cagctcgtgg ctgtgcatat ccaaggcaag gcggcggacg ttgcggacga
     6121 cggtgaggat gtggtcgtcc acgggataga tgtggaacag gtcgtgctgg agcaggccga
     6181 cgattttttc ccacgcgggc aggtagcgtc ccaatacgcc gtagaggttg aggaagcgca
     6241 gggtttgggt cagtccgttg ccgttgcgga aaaagccggc gaagcggtgg cggttttcag
     6301 aattttggta gaagctgcgg ttgattttgc gcgtcgcgcc ccaccatgcg cgcagggttt
     6361 gcggttcgag cgcggtaata tcgttgcgct gctgcatgat ttcgacgatt ttgaaaatgt
     6421 gttcgggccg tctgaaaaaa atatcggtgt gccgcgcggc gatttggttg ttgacttgga
     6481 tgtagtcgtc gtcaatccgc agggtaacgc gcaacggggt ggaggaaacg cggctttgca
     6541 gcataggcgt gaggatgccg ctcagttgtt tgacggtttt aatcgcgcgg taaaacacgc
     6601 gcatcagttc ttcgctttga cggcggaggt tcaagccttg ataacccata ctttcggcga
     6661 cttgcggctg caaatcgaac agcaggcggt cttcggcgcg cttggcgttt aaatgcagat
     6721 ggatgcggat gtgggcgagg cggcggtagc cgtgtgaaag cataccggct tcagtgcgcg
     6781 tcagaatgcc ttgtttgacc agcgcaggca ggttggtcgc caagccttgt gctttcgcta
     6841 tccaaagcag ggtatggatg tcgcgtagac cgccgggaca gcttttgatg tttggttcca
     6901 acaccgctcc cgaaccttgc gatttggcat ggcggtgttc catctccacc aattttgctt
     6961 cgataaatgc cgctacattg cgttgcgcgt tcattttttc cgccagttca tccgccgttt
     7021 ggcggttgcc aaacaaaaaa cgggcctcta aaaacgctgt gtcccccgta atatcattgc
     7081 gcacgctttc gcacagttcg tcaatgccgc cgctttttac agacggcatc agtttgcagt
     7141 cccacagggt ttggacaaac cgggcaatct gttcctgaat gccgtctgaa agcggggcag
     7201 gggagacaac cgccaaatcc acatccgaac agggatacag ttcgccgcgc ccgaagccgc
     7261 ccaccgccat cagacataac gcgctgtttt gaaaatgttc tacccacaat gctgccagta
     7321 aggtttcgac tgccgccgtg tattccctga aaaataccga cacgcggttg gttttcaaat
     7381 aatgcgcttc ggcggcatcg cgctgctgtt tgaaggtttc cagtgctgaa gacaggtttg
     7441 caggcatttt tattctttcg attggcggga aaaagggagg cggatggttc agcggtcaaa
     7501 taccgctttc agacggcatt tgtcgggtat cggcggaatt ggtcatcagg ttcaaccgtt
     7561 cctgcggcgg cagcagcaac tgatacgccg ccacgcccaa ggccgccgcc attttttcga
     7621 tattcgacag ggcgatgttc cagcgtttgc gctcgactgc cgacacataa gtcctgtcca
     7681 aaccgcattg ccgcgccaat tcttcttgcg accaaccctt gttcacgcgg aaaagccgca
     7741 tattgtatgc caataccgcc cgcaaatcct gctcgtcagg cagttcggca ggcagagtca
     7801 atttgttgcc catcatttgt ttccggataa atgttaaagt taagcatttg ctgttacgga
     7861 ttttacttca cataaaagct aaaaaaaagg ggtggcggca gccacaccca aacacacaca
     7921 catcaagagg aaagaggtaa aatcaagaca gattgggaaa cagcaaacaa acaatctaga
     7981 tttcatgtcg gtattatcaa acaaaaaccg ttatcgagta ttgattcaaa tcaatttcga
     8041 ttttattcgc aatccgataa agaaaaacgg catatccgtt tatatggata tgccgtctga
     8101 tggtgcaggg gactgctata tttggcagtg atttgtgttt tagtcctctt gcgccgctgt
     8161 ttgcaggtag ttcggcaaac cgattttgcc gatcagttcc tgctgggttt cgagccagtc
     8221 gatgtgttct tcgttggtgt ctttttgttt ttccaacaaa tcacggctga cataatcctg
     8281 ttgcgcttct gccacggaaa ttgccgcaat caatgcctca tgtttttctt tctctttttc
     8341 caaatcgcag gcgatgattt cttcggtgga ctcgccaatc agaagcttgc ccagttcttg
     8401 caggttcggc agaccttcga ggaacagaat gcgctcgatc aaatcgtcgg cagctttcat
     8461 ttcaacgatg gactgtttga agaaatgttc gcccagttct tcaaagcccc agtttttcaa
     8521 aatacgggcg tgaaggaaat attggttgat ggttaccagc agcaagccta agtttttgtt
     8581 tagctcgcgg ataaccagac ggtcgccttt catacggact ccttttattc cgaatacgga
     8641 ttacagttgg ctttggtagt agttgccttc gccaattaac tcgatcaggc gaagctgctg
     8701 ttccagccag tgggcgtggt cttcttcggt atctttcagt tgggcaacca tcaggtcgcg
     8761 cgtaacatag tcttgagcct cttcgcacag tttgatgcct tttttcaacg catcacgtac
     8821 ttcatattcg gtttgcaggt cggctttgag gcaggaaacc acgtccgtgc cgatattcag
     8881 ttcggcgcgt gccattttcg gcgtgccgcc cagcatcagg atgcggcgga tgaagtcttc
     8941 ggcgtgtgtg gtttcttctt ccatctcgtg gttgagacgt tcaaaaagtt tggtgtagcc
     9001 ccattcggag tagaggcggg agtggataaa gtattggtcg cgtgccgcca gctcgccaga
     9061 cagcaattcg ttcatgtaat ctaaaactgc ttgattgcct tgcataatat ttctcttttc
     9121 ttaatttggg tttcgtgtag ttaaacagtg gatgtagcac tgttcggaat gcgtgcattg
     9181 tatgcaaaag ctgtcggcat gacaaatttt ctatttaaaa tacaaacaat tatcaaaaga
     9241 aatagggcgt attccccgat gccttccaaa tcagtatgct gttttttatc ggttttttgt
     9301 gctgttaaaa ctaataatcc atattatcca taaagaacat ttacacttgt taaccatagc
     9361 taaaagcaaa tacggtacgg ttttttatca aaatttataa tgaatcgttc tcattaaccg
     9421 acagattctt ttgagattat cggattttgg gaataaatta tgccgtctga ggggctttca
     9481 gacggcatag acaattaaag ctgattaaac aacgcggcag acagcagcgt aggaaattat
     9541 tttttacttc ggaacggtat tttgggttta atgatgcccg caaccgccgt ggctttcgcg
     9601 taaggcttcg gcagcctgtt cgtcggcgtg gtagctggaa cgtaccatcg cgccgatggc
     9661 ggcattgctg aagcccagtt cgtatgcttc tttttcaaag attttgaact gctcgggcgt
     9721 aacgtagcgc aggacgggca ggtgtccgtc tgaaggctgg aggtactgtc cgatggtaat
     9781 catttcgata ttgtgcgccc gcatatcgcg cataatttca cgcacgtctt cgtctgtttc
     9841 gcccaagccg accatgatgc cggatttggt cgggatgtgc ggcatcattt ctttataacg
     9901 ttttaataag tctaaagaat gttgataatt ggcaccggga cgggcttttc tgtacaggct
     9961 cggatgggtt tctaggttgt ggttcatcac gtcgggcggg gtttcggcaa ggattttgag
    10021 tgcgatgtcc aagcgtcctc ggaagtcggg gacgaggatt tcgattttgg tgttcgggct
    10081 ggtttcgcgg atggctttga tgcagtcggc gaaatgctgt gcgccgccgt cgcgcaggtc
    10141 gtcgcggtcg acggaggtga tgacgacgta acgcaggttc atggctttga cggattcggc
    10201 gaggtttttc ggttcgtcgg ggtcgagcat attgggccga ccgtgtccca cgtcgcagaa
    10261 cgggcagcgg cgggtgcaga tatcgcccat aatcatgaag gtcgccgtgc ctttgctgaa
    10321 gcattcgccg atgttggggc aggaggcttc ctcgcaaacg gtgtgcatct tttgttcgcg
    10381 caaaatgtct ttgatttcaa agaatttgcg cgatgggagt ttggcgcgta tccattcggg
    10441 ctttttcagt ttttcctgaa gggggacgac tttgatgggg atgcgcgcgg ttttgtccgc
    10501 gcctctgagt ttgatgccgc gtttggggtc gtcggttttg atttcactca ttgttatctg
    10561 ctttcggtgt gaattgtgtt tcaaggtgtg cggtaagttt ggcggcgact tcgtccggcg
    10621 tggggcaggg ttggacaaaa tccgcgattt gcgtcatttc cataccggcg tagccgcagg
    10681 ggttgatgtg ggtaaacggg cttaaatcca tattgacgtt gagcgcaagg ccgtgataga
    10741 cggaacctcc tttgatacgc agccccagtg aggcgatttt gcgttcgccg acgtaaacgc
    10801 cggggcgttt gggatctgcc gccgcttcga tgccgtattc cgccaatgtg gcgatgatgc
    10861 tgttttcaag cgcggaaaca atgttgcgga cgctggtttt gcgccgtttg aaatcaatca
    10921 tcgtataaac gaccaattgc ccgggtccgt gataggtaat ctgcccgccc cggtcgattt
    10981 ggacgatggg aatgtcgtcg cggatcagca ggtgttcggg tttgccggcc agcccctgtg
    11041 tgaacacggg gggatgttct accacccaca attcatcttc ggtagaggcg gtacggttgg
    11101 cgttgaaagt tttcattgcc tcaaaagtcg gcagatattc aaccaaacct ttatgtatga
    11161 ttttcatctc aaagtaccac tttgaccaat ttgtgcgaag tcagcgcgcg gtagatattg
    11221 tcaagttgtt cttggttttc aacctttact tgtacggtgg cgccggtata gttgcctttg
    11281 ctgctcggac gcgtggtgat gtggtgcgcc tgcgtgtcgg gggcgtggag gcggacggtg
    11341 tccaataccg cctgctcgaa ctcgggatgc accgcgccca ttactttcaa tgggaaggtg
    11401 cagggaaatt cgatgaggga tgttttgttt ttttgttcgg tcatgatgtg ctgccttgtc
    11461 gtgtacggta tgccgtctga aggcgggttt gcctttcaga cggcatcgga tgtgcgttat
    11521 tttagcctaa accgcgataa caggctatcg ggaaggcgga ggcttttttg acggcgcggc
    11581 ggttctgcta tactggcgca caatattatt tttagaaggg tggtttttat gtatcggagg
    11641 aaagggcggg gcatcaagcc gtggatgggt gccggtgcgg cgtttgccgc cttggtctgg
    11701 ctggttttcg cgctcggcga tactttgact ccgtttgcgg ttgcggcggt gctggcgtat
    11761 gtattggacc ctttggtcga atggttgcag aaaaagggtt tgaaccgtgc atccgcttcg
    11821 atgtctgtga tggtgttttc cttgattttg ttgttggcat tattgttgat tattgtccct
    11881 atgctggtcg ggcagttcaa caatttggca tcgcgcctgc cccaattaat cggttttatg
    11941 cagaacacgc tgctgccgtg gttgaaaaat acaatcggcg gatatgtgga aatcgatcag
    12001 gcatctatta ttgcgtggct tcaggcgcat acgggcgagt tgagcaacgc gcttaaggcg
    12061 tggtttcccg ttttgatgag gcagggcggc aatattgtca gcagtatcgg caacctgctg
    12121 ctgcttccct tgctgcttta ctatttcctg ctggattggc agcggtggtc gtgcggcatt
    12181 gccaaactgg ttccgaggcg ttttgccggt gcttatacgc gcattacagg caatttgaac
    12241 gaggtattgg gcgaattttt gcgcgggcag cttctggtga tgctgattat gggtttggtt
    12301 tacggcttgg ggttggtgct ggtcgggctg gattcggggt ttgcaatcgg tatggttgcc
    12361 ggtattttgg tttttgttcc ctatttgggc gcgtttacag gactgctgct ggcaaccgtc
    12421 gccgccttgc tccagttcgg ttcgtggaac ggcatcttgg ctgtttgggc ggtttttgcc
    12481 gtaggacagt ttctcgaaag ttttttcatt acgccgaaaa tcgtgggaga ccgtatcggg
    12541 ctgtcgccgt tttgggttat cttttcgctg atggcgttcg ggcagctgat gggctttgtc
    12601 ggaatgttgg caggattgcc tttggccgcc gtaaccttgg tcttgcttcg cgagggcgtg
    12661 cagaaatatt ttgccggcag tttttaccgg ggcaggtagg cggttccgaa acatatttga
    12721 agcggaatac aaccctgccc gagtttaa
//
LOCUS       DAWFAD010000050        12350 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWFAD010000050 DAWFAD010000000
VERSION     DAWFAD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.050"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(125..976)
                     /locus_tag="WGO49_001030"
     CDS             complement(125..976)
                     /locus_tag="WGO49_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ0306147.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDARFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEAACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLQHALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESLEIPAA
                     HKRKTAE"
     gene            1324..2838
                     /gene="katA"
                     /locus_tag="WGO49_001031"
     CDS             1324..2838
                     /gene="katA"
                     /locus_tag="WGO49_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0306148.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            2914..3051
                     /locus_tag="WGO49_001032"
     CDS             2914..3051
                     /locus_tag="WGO49_001032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306149.1"
                     /translation="MPSETSFDRFRRHFPIPPPPFQWAFFIKRKISRHSHAGGNLERK
                     I"
     gene            3371..3619
                     /locus_tag="WGO49_001033"
     CDS             3371..3619
                     /locus_tag="WGO49_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306150.1"
                     /translation="MHRTGDRVDVGWASAHQSHHSTNSANLTGAGKPMPSEGGGIGGG
                     GGLKPTLQPPHPQKNQKKPNLTKSDKKRQPETDKNCHN"
     gene            3877..4323
                     /locus_tag="WGO49_001034"
     CDS             3877..4323
                     /locus_tag="WGO49_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0306151.1"
                     /translation="MKAIQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQMSEAL
                     TLAEGQKSAVVEYYSDNGTFPNSNTSAGIAASNEIKGKYVASVKVEGNASVASITATM
                     NSSNVNKDIKGKTLVLVGKQNSGSFSWECKKGSVGEKFLPSTCRTK"
     gene            4777..5277
                     /locus_tag="WGO49_001035"
     CDS             4777..5277
                     /locus_tag="WGO49_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0306152.1"
                     /translation="MSNYRRDFTRGAHYFFTLALQDRTKDYLIRHIDRLRLAYRETKT
                     RYPFETAAICILPDHLHFIMRLPEGDHDYPIRIAYLKTRFSQLIPKECRAPNGSKTKK
                     REAGIWQRRFWEHLIRDEQDLNNHRDYIYFNPVKHGYAASVKEWRYSSFFKDVEKGIY
                     PLDWGG"
     gene            5741..6307
                     /locus_tag="WGO49_001036"
     CDS             5741..6307
                     /locus_tag="WGO49_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ0306153.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMHEGLSGEAEDARENKRES
                     WWLTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVLVAVGLTVGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIG"
     gene            complement(6583..8619)
                     /locus_tag="WGO49_001037"
     CDS             complement(6583..8619)
                     /locus_tag="WGO49_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ0306154.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSESKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLGLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGKTIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
     gene            complement(8707..9579)
                     /locus_tag="WGO49_001038"
     CDS             complement(8707..9579)
                     /locus_tag="WGO49_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0306155.1"
                     /translation="MEIWNRLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            9849..12239
                     /gene="gyrB"
                     /locus_tag="WGO49_001039"
     CDS             9849..12239
                     /gene="gyrB"
                     /locus_tag="WGO49_001039"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ0306156.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLNV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIVSDSRTIEGEELANTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
BASE COUNT         2907 a         3312 c         3424 g         2707 t
ORIGIN      
        1 gtttctagat tccctctttc gcgggaatga cgggatgcag gtttccgcgc ggacggattc
       61 ggcattcccg ctttcgcaga aatgccggct tttggtttgt tgtctttata agattattca
      121 gcaattattc tgcggttttg cgtttgtgtg ctgccggaat ctcaagggat tctctgtatt
      181 tggcaatggt gcggcgagaa acctccatgc cgcggaaagc cagcaggttg gcgagtgcct
      241 cgtcagaata cggcgtgggt gtggacgagg caaaaccggc ggcgggaaag ggattgagcg
      301 aagcaatgag gtcgagtgcg gcttcgagtg tgccgctgtc ggtttggggc aggtgttttt
      361 ttattcgtgc gagggtttgg ctgcggttgc cgtcaatgct gtcgagggcg tttcggacga
      421 tatgcagggc ggcgggtttg gcagcacatt cgcccgatct ttctatctgc agtatcagcg
      481 attcgttcaa atcggcggcg gccacgcctg ccgggtcgaa ttttttcaat gcggtcagcg
      541 cgtgttgcag cattgcttca tccaacatcc actctaaggg cgtatggtcg aggatgtctt
      601 cgatgctgtc ggtcagataa ccctgctcgt caaggaaatc gataaggatg tggacacagg
      661 cggcttcttg gtcggaaagc gggtgttcgc atacttgcgc gtgcaggtat tgcttgaaat
      721 cctgctcacc ggcgatgttg gacagcatat cttcgccttc gtctccgccg atttgacggg
      781 caggcgcggt gtaatgggaa aacctagcat cggaaaattc atccgtgtct ttgcgttcga
      841 gcagggggtt gtccgacagc cagttttcga cctcgcgttc aagttcgata cccgacatct
      901 gcaatacgcg caaagattgt tgcagccgct ggttgagctg ctgggtctgt ttgagcttta
      961 ttccgagtaa ggtcatgata atgtggggaa aattgttatt ttcagcctgt cggcgcaaaa
     1021 aatgccgcaa agcgtcattg cattataaat ggttttaatg agcgggttcg gattccgttc
     1081 gataacaaaa aacaaacgaa aatcaagaac cgattgctta taataatatt aaatcgattt
     1141 catagtttta atagcgaaaa tcttggcgta tagtcgcatc catagttttt acaaaaggga
     1201 aacaaaatgt cgattcaaga aatttattgc aatcaagaaa ccggttacga atacgctttc
     1261 cgccaaatcg tactgtaagc cgtttacccc catttgacca acctgagaaa aggaacaaga
     1321 gcgatgacta cctccaaatg ccccgtaacc catctgacca tgaacaacgg cgcgcctgtt
     1381 gccgacaatc aaaacagcct gaccgccggt cctcgcggcc ctctgctggc gcaggatttg
     1441 tggctgaatg aaaaactcgc cgacttcgtg cgcgaagtca tccccgaacg ccgtatgcac
     1501 gccaaaggtt cgggcgcgtt cggtacgttt accgtaacgc acgacatcac caaatacacc
     1561 cgcgccaaaa tcttcagcga agtcggcaaa aaaaccgaga tgttcgcccg tttcaccacc
     1621 gtggcaggcg aacgcggcgc agccgatgcg gaacgcgaca tccgcggctt tgccttgaag
     1681 ttctataccg aagaaggcaa ctgggatgtg gtcggcaaca acacgcccgt gttcttcctg
     1741 cgcgacccgc gtaagttccc cgacctgaac aaagccgtca aacgcgaccc gcgcaccaat
     1801 atgcgctctg ccacaaacaa ctgggacttc tggacgctgc tgcccgaagc actgcaccaa
     1861 gttaccatcg tgatgagcga ccgcggcatc cccgccggct accgccatat gcacggcttc
     1921 ggttcgcata cctacagctt ctggaacgaa gcaggcgagc gtttttgggt gaaattccat
     1981 ttccgcaccc aacaaggcat taaaaacctg accaacgaag aagccgccaa aatcatcgcc
     2041 gacgaccgcg aaagccatca gcgcgactta tacgaagcca tcgaacgcgg cgagtttccg
     2101 aaatggacga tgtacatcca agtcatgcct gaagcagacg cggaaaaagt accttatcat
     2161 ccgtttgact tgaccaaagt ttggccgaaa aaagactatc cgctgattga agtgggcgaa
     2221 ttcgagttga accgcaatcc cgaaaacttc ttcgccgatg tggaacaatc cgccttcgca
     2281 ccgagcaacc tcgttcccgg tatcggcgcg agcccagata aaatgttgca agcccgtttg
     2341 ttcaattacg ccgacgcgca acgctaccgc ttgggcgtga acttccgcca aatccctgtc
     2401 aaccgtccgc gttgccctgt tcacagcaac cagcgcgacg gacaaggacg cgccgacggc
     2461 aactacggca gcctgccgca ctacgaaccc aatagcttcg gccaatggca gcaacaaccc
     2521 gacttcgccg aaccgccttt gaaaatcaac ggcgacgcgg cgcactggga ctaccgccaa
     2581 gacgatgacg actatttcag ccaaccgcgc gccctgttca acctgatgaa cgacgcgcag
     2641 aaacaggcat tgttcggcaa caccgccgcc gcaatgggcg acgcgcccga cttcatcaaa
     2701 taccgccata tccgcaactg ctaccgttgc gatccggcat acggcgaagg cgtggcaaaa
     2761 gccctcggac tgactgtcga agacgcacaa gccgcccgcg cgaccgaccc cgcactcggc
     2821 cagcccggtt tgctgtaagg gggcattatg tggatggaaa ttgaagaaat cctgtcccgc
     2881 gccgtccgct accggaaata accgggcata aaaatgccgt ctgaaacgtc gtttgaccgc
     2941 ttcagacggc atttccccat cccgcccccg ccgtttcagt gggcgttttt tattaaacgc
     3001 aaaatatccc gtcattccca cgcaggcggg aatctagaac gtaaaatcta aagaaacctt
     3061 tttccccata agtttccgca ccgacagacc tggattcccg cctgcgcggg aatgacggtt
     3121 taggtatttc tgacgattca gtaagccgta tcggactgta gaatgggctg taaggtgggc
     3181 gtagggtggg cttcagccca ccaatcccac caacccactt ttccgcgtca ttcccacgaa
     3241 agtgggaatc tagaacgtaa aatctaaaga aacctttttc cccgataagt ttccgtgccg
     3301 acaggtctgg attcccacct gcgcgggaat gacggtttag gtattcacat aaggcggatg
     3361 tgtaaggtag atgcatagga cgggcgatag ggtggacgta gggtgggctt cagcccacca
     3421 atcccaccat tccaccaact ccgccaatct taccggtgcc ggcaaaccga tgccgtctga
     3481 aggcggcgga atcggcggag gcggtgggct gaagcccacc ctacagccac cccaccccca
     3541 aaaaaaccaa aaaaaaccca atctgacaaa atctgacaaa aaacgtcagc ccgaaactga
     3601 caaaaattgt cacaactgac aagaaacgtc agcccgccgc attattacgc cgcaagccgc
     3661 gccgcgttga tataaaaaaa tacccatacc agacagacaa ataaaaaaat attctgtaaa
     3721 atgccgagct gaaccctgtt ttggcacgcc gcttgctggt aagaaggcga accgaagtaa
     3781 aaaaaaccac ggtcaaatac attacgggtt tacgtttgcg gcggatgccg caaatttcca
     3841 atcaaactta aacattttca aacaggagtc atccaaatga aagcaatcca aaaaggtttc
     3901 accctgatcg agctgatgat cgtcatcgcc atcgtcggta tcttggcagc cgtcgccctg
     3961 cccgcatacc aagactacac cgcgcgcgcc caaatgtccg aagccctgac tttggcagaa
     4021 ggtcaaaaat ccgcagtggt cgagtattat tccgacaacg gcacattccc gaacagcaat
     4081 acttccgcag gtattgctgc ctctaacgag attaaaggta agtatgtggc atcggttaag
     4141 gttgaaggta atgcctctgt tgcttctatt accgctacca tgaactctag taatgtgaat
     4201 aaggacatca aaggtaaaac cttggtactc gtcggcaaac aaaactccgg ctcgttctct
     4261 tgggaatgta aaaaaggttc tgtgggcgaa aaattcttgc catctacctg ccgcaccaaa
     4321 taaggacaat gaccgggttt gacccggtcg tgaaaacaaa tgccgcccga accgctcggg
     4381 cggcattttc tattcccctt tgccgctgta gggctgtagg gtgaggcgta ggtgcgtagg
     4441 gtgggctgta gggtgggctt cagcccacca ttccaccaac ccaaccaact ccaccacatc
     4501 aaggattttt ggcgaaccgt tgggattcgg cgggtttcga gtttcggcgg ggattggcgg
     4561 aatggttggt ttgggttggg tgggaatggt tggattggcg gaatggtggg ctgaagccca
     4621 ccctacgaaa caccctacga aacaccctac agcctacaca tcagccctac gccctcccta
     4681 cccatttccc tgcaaatccc ctaaaaatcc tcccgcacgc cctgaaaaca aggaataata
     4741 caggtttccg cccatcgggc taaaaagggg ataaatatgt caaattaccg gagggacttc
     4801 acgcgcgggg cgcactattt tttcacattg gctttgcaag acaggacgaa ggattacctg
     4861 atacggcata tcgacaggct gcgcctcgcc tatcgtgaaa cgaaaacacg ttatccgttt
     4921 gaaacggcgg caatttgtat tttgccggat cacctgcatt tcattatgcg gctgccggaa
     4981 ggcgaccacg attacccgat acggattgca tatttgaaaa cccggttttc ccaactcatc
     5041 cccaaagagt gccgcgcccc caacgggagt aaaacaaaaa aacgcgaagc cggcatttgg
     5101 cagcgcaggt tttgggaaca cctgatccgg gacgaacaag atttaaacaa ccaccgggat
     5161 tatatttatt tcaaccccgt gaaacacggg tatgcggcga gtgtgaagga atggcgttat
     5221 tcttccttct tcaaagacgt tgaaaaaggc atttaccctt tggattgggg agggtaaagc
     5281 tcccgccgcc gccaaaatcc gcaggacgga cgcggggcag atgagtcggg cgggacgtag
     5341 ggtgaggcgt aggtgcgtag ggtggatgtg tagggtgggc ttcagcccac caatccaacc
     5401 acctccgcca atccaaccat tcccaccaac ccaaaccaac cattccgcca atccccgcca
     5461 aaaaccgaaa ccgccgaatc ccgacggttt gccaaaaacc cttgatgcgg tggaattggc
     5521 gggattggat gagttggttt ggcagaattg gtgggctgaa gcccacccta cagcccaccc
     5581 tacaacccgc cctaagtccc acctacgttc catccgacgc atccgcccta cggttccgga
     5641 ttcccctgcg aaccgccccg cgcacgaaaa ctgctttacg cggcgttttt cgcgttttgc
     5701 tgttttgcta taatccgccc tttttaagga cgggtgcggt atgagtcttt acgctttgct
     5761 cttgattgcg ctggggatgt cgatggatgc gtttgccgtc gcgctggcaa agggtgcggc
     5821 ggtcaggatg cctccgcgca aaattgcagc aacagccttg gtgttcggca cggttgaagc
     5881 gctcacgccg ctggcaggct gggtaggcgg tttttatgcc aagccgttta tcagcgaatg
     5941 ggaccattgg gcggctttcg tcctgctggg cgggctgggt ctgaaaatga tgcacgaagg
     6001 gttgtccggc gaggcggagg acgcgcggga aaacaaacgg gaaagctggt ggttgacggt
     6061 tctgactgct tttggaacca gtattgattc catgatagtc ggggtgggct tggcgtttat
     6121 ggaggtaaac atcgcctttg ccgccgcaat catcggtatg gcgacgacgg tgctggtggc
     6181 ggtcgggctg acggtgggaa gggctttggg cgtattgttc ggcaggcgtg cggaatttgc
     6241 cggaggtttg gtgttgattg ccatcggtac atggacgctc ttgtcgcact tggggctgat
     6301 cgggtaagga ctgccaaaaa atgctgtctg aagcgttgtt tgaccgtttc agacggcatt
     6361 ttttattttt tgggcgtagg gcgaatccgt aaggtgggat gtaaggtggg atgtagggtg
     6421 ggcttcagcc caccctacgc ccactgtttt tgattttttg tttttgcggg aatgacggga
     6481 ttttggctta ctgtttttgt gaaaatggcg aaaccgcgca aatgctgatg ccgtctgaaa
     6541 cactgtttga ttgtttcaga cggcattttt tgttcacatc gatcaagccg cgttgtcgaa
     6601 accgctgtgg cgcaagagtg cgtctatgct cggttcgcgg ccgcggaagg ctttgaagga
     6661 ttctgccgcg ctgcgcgatc cgccgacggc gaggatttcc tgccaaaagc gtttgcctgt
     6721 ggcggcgaca tcgtcgcttt cttcaaaggc ggcgtatgcg tccgcgctca atacttccgc
     6781 ccacgcgtag ctgtaatagc ctgcggaata gccgcctgcg aagatgtggc cgaagctcaa
     6841 ggcgaagcgg ttgtattcgg gcggctggat gacggcgact tttttgcgca cgctgtctaa
     6901 aacctgttgc cagtttttca gacggccttc gtcgtcttcg ctgtaaatca tcatatcaaa
     6961 gagggcgaac tccatttgcc ggacgaggaa catgccgcgt tggaagtttt tggcggcgag
     7021 cattttgtcg aagagttctt tcggcagggg aacgccggtt tcttcgtggg ctgacatttg
     7081 tgccaagaca ttgtattccc aaacgaaatt ttccataaac tggctgggca gttcgaccgc
     7141 gtcccattct acgccgttga tgccggatac gcccagttcg tccacttggg taagcaggtg
     7201 gtgcagcccg tgtccggttt cgtggaagag gatgaggatt tcgtcgtggc tcaggcgggc
     7261 ttccctgccg ccgacgggtg gggcgaagtt gcagacgagg taggcggtgg gcagttgcag
     7321 cgtgccgtct gaaaaacggc ggcggccttt gtagtcgttc atccacgcgc cgccgcgctt
     7381 gccttcgcgg gcgtagaggt ccatatagac gccgcctatg gttttgccgt tttgttgcaa
     7441 ttcaaaatag cgcacgtctt tgtgccagac ggggacggtt ttttcggtaa atccgatgcc
     7501 gtagagtttt ttgatttggg cgaacagtcc gtttaatact ttgccgacgg ggaagtattt
     7561 tttgacttcg gtttcgctga acgcgtattt ggcttcgcgc agtttttcgc tggcgtagcc
     7621 caagtcccac ggttgcaaat cggcgaggcc gaggctttcg cgggcgaagg ctttgacttc
     7681 ggcgaggtct ttttcggcgt agggtttggc gcggcgggcg aggtcgtgca ggaagtttaa
     7741 aacttgttcg ggggtgtccg ccattttggt tgccagcgac aattcggcgt agtttttgaa
     7801 gccgagcagt ttggcggttt gcagggcgtt tgcgagcgtg cggtcgatgt tggcggtgtt
     7861 gtcgaatttg ccgtcgtctg acagttcgct ggcgcgggta acgtaggcgc ggtagatttg
     7921 ttcgcgcagt tcgcggttgt cggcgtattg gatgacggcg aggtagtgtg gaatctgcaa
     7981 gccgattttg tagcctgttt tgctttcgct ttgcgcggcg gcggcaaaca tggcgagcgc
     8041 gtcttcggga atgccggcaa gcggtgcggc atcgtcaaag taaatgccga acgcgtcggt
     8101 cgcgtctagg acgttttggg agaatttggc ggaaagttgc gcgccttcgg tttgcagttt
     8161 tgccagttct gcctgctgtt cgggcggcag ttccgcgccg ctgaggacga aatcgcgcag
     8221 atcgtggttg agtttggttt tttgtgcggg ggagagggtg tcgaattcgg gggaattttt
     8281 gatggttttg aagcggttgt acagctcgat gtcttgtccg atttcggtga agaagacggt
     8341 gatttcgggc atcagttcgt tatagacggc gcgcagttcg ggcgtgtcgg cgacggagtt
     8401 gaggtgcgac accacgcccc aaatcctgcc gacgcgttcg gtgatgccgg tcaggggttc
     8461 gacagtgttt gcccagccgg tgtgcgtttg ggctttgatg gcggcgattt gttcgcgcgc
     8521 ttcggcgatg gcggtttgca gggcgggttt gatgtcttcg gttttgattt gatcaaaacg
     8581 gggttcttcg cccaaatgga gcagtgcgtt gtcagtcata agatgggttt cctttgcgtg
     8641 ttgtgcttca gacagcatat cggcaatatg ccgtctgaag ccggaaatgg ggtcggtcgg
     8701 ggaaagttaa agtgtttcgg agccggcggg gtaatttaac aggcggtatt gtacgcgcaa
     8761 aaattcgtcc ataaccgcct gttggatttc cagccgcttt gaaacggggg aggcgaagcg
     8821 gacgatgatg cggtatgcct tgtcatcgta cggcacgcgg gtaacgcgcg gtctggcggc
     8881 gggcgtgata aacagttttt ccgcctgcac gttttccaaa tgccgttgga tggcggggat
     8941 gtagggcgcg cacaagggct cgagtacggc tttcagacgg catacggctt catccgaatc
     9001 caaatggatg gggacgggga tttcgaccgt atggatgaca tagtcgccca aaatattgtc
     9061 gcggcgcacg gggtggctca acaacaggct gttggggaaa gaaacggtgg ttcccgcaag
     9121 ctgtccgacc aaggggttcg gaccgacctg catcatcagc gtgttcaaca ggttgatgtc
     9181 gaccacgcgc ccgcgcaggc cgttgatttc gatatagtcg ccgaccgagt attgctgggt
     9241 ggcagacctt aaaatactgc ccgacagaca cataatcagt tccttcgtcg ccacgacgac
     9301 cgccgccgcc accgcaaaca tcgacaaagc cagcgtttgg atttgcgccg accagataaa
     9361 tgccagcgaa aacagcacca aaagcagcgt tatattgcgg ctggcaacca aaaaccgccg
     9421 cttgctttcg atgccgaaat ccggatgccg tttgaagtgg atattcaaca gaagggcgcg
     9481 cgccagcagc aaagccgcaa ccgccgccac ggattcgacc gcctccgcac gtatcgggac
     9541 ggcaccgagc caagtgtcca acctattcca tatttccatt tccgcgcccc tgtccgaaaa
     9601 gtatagggtg tagattttag tggcaaaaaa acgcttttgc cactttacgg gaaaatcccc
     9661 gacctgatgc cggaggcaaa cgtaccgttt catcttttcc cggcggcaca ctgatgcgga
     9721 taacgctgaa ttaggcggca aaatcggcta taatacacaa ttatatatgc cgtcccgccc
     9781 gctttttcca caaggtgcgg acttgttttg gcggcatcgc aaaatctttt caaaatccgg
     9841 caaaaaatat gactgaacaa aaacacgaag aatacggcgc cgacagcatt caggtgctcg
     9901 aaggcttgga agcggtacgc aaacgccccg gcatgtacat cggcgacacg caggacggca
     9961 gcggcctgca ccatatggtg ttcgaagtat tggacaacgc cattgacgaa gcactcgccg
    10021 gacattgcga caaaatcacg gtaacgatac acgccgacca ttccgtcagc gtcgccgaca
    10081 acgggcgcgg tatgcccacc ggcatccacc cgaaagaagg acgctccgcc gccgaagtca
    10141 tcatgaccgt attgcacgcg ggcggtaaat tcgacaacaa cagctacaaa atctccggcg
    10201 gcctgcacgg cgtgggcgtg tccgtcgtca acgcgctgtc cgactgggta acgctgacca
    10261 tctaccgcga cggcaaagaa cacttcgtcc gcttcgtgcg cggcgaaacc gaagagccgc
    10321 tgaacgttgt cggcgattcc gataaaaaag gcacgaccgt gcgcttcctc gccagtacgg
    10381 aaaccttcgg caacgtcgaa tacagcttcg acatccttgc caaacgcatc cgcgaacttt
    10441 cgttcctgaa caacggcgtg gacatcgaat tgaccgacga gcgcgacggc aaacacgaaa
    10501 gcttcgccct ttccggcggc gtggctggat tcgtgcaata catgaaccgc aaaaaaacgc
    10561 ccttgcacga aaaaatcttc tacgcgttcg gcgagaaaga cggcatgagc gtcgaatgcg
    10621 caatgcaatg gaacgacagc tatcaggaaa gcgtgcagtg cttcaccaac aacatccctc
    10681 agcgcgacgg cggtacgcac ctgaccgcgt tgcgccaagt gatgacgcgc accatcaaca
    10741 gctacatcga agccaacgaa gtcgccaaaa aagccaaagt ggaaaccgcc ggcgacgata
    10801 tgcgcgaagg tttgacctgc gtgttgtccg tcaaactgcc cgaccccaaa ttttcgtccc
    10861 aaaccaaaga caaactggtt tccggcgaaa tcggtcccgt cgtcaacgaa gtcatcagcc
    10921 aagccctgac cgacttcctc gaagaaaacc ctgccgaagc caaaatcatc accggcaaaa
    10981 tcgtcgatgc cgcccgcgcg cgcgaagccg cccgcaaagc ccgcgaaatc acccgccgca
    11041 aaggcgtaat ggacggcttg gggctacccg gcaaactcgc cgactgccaa gaaaaagacc
    11101 ccgcattgtc agaactttat ttggtcgagg gcgattctgc aggcggttcc gccatgcagg
    11161 ggcgcgaccg caaattccaa gcgattttgc cgcttaaggg caagattttg aacgtggaaa
    11221 aggcgcgctt tgaaaaaatg ctcgccagtc aggaagtcgc cgctttgatt actgcgttgg
    11281 gtgcaggtat cggcaaggaa gaattcaatg ccgagaagct gcgttaccac cgcatcatca
    11341 ttatgaccga tgccgatgtg gacggcgcgc acatccgcac cctgctttta accttcttct
    11401 accgccaaat gcccgagctg gtcgaacgcg gctacatcta cattgcccag ccgcctttat
    11461 acaaagccaa atacggcaaa caagaacgat atctcaaaga cgaattggaa aaagaccaat
    11521 ggctgctcgg tttagccttg gaaaaagcca aaatcgtttc agacagccgc accatcgaag
    11581 gcgaagagct tgccaatacc gccaaacaat tcctgttggc caaaaccgtc atcgaacagg
    11641 aaagccgctt cgtagacgaa ctcgtcctgc gcgccatgct acacgcgtcg cccattgatt
    11701 tgacgtcgtc tgaaaacgcc gataaagccg ttgccgaact ttccggtctg cttgacgaaa
    11761 aagaagtcgc cctcgaacgc atcgaaggtc atgaaggaca ccgattcatc aaaatcacgc
    11821 gcaagctgca cggcaacgtc atggtcagct acatcgaacc caagttcctc aacagcaaag
    11881 cctaccaaac cctcacccaa accgccgccg cgctcaaagg catggtcggc gagggtgcca
    11941 agctttacaa aggcgaaaac gggtacgacg cggacagctt tgaaaccgct ttggacatct
    12001 tgatgagcgt tgcccaaaaa ggtatgtcca tccaacgata caaaggcttg ggcgagatga
    12061 accccgaaca gctgtgggaa accacgatgg atcccgccgt gcgccgcctg ttgaaagtgc
    12121 gcatcgaaga cgccatcgcc gccgacgaag tgttcgttac gctgatgggc gacgaggtcg
    12181 agccgcgccg tgcctttatc gaaaacaacg cgctgattgc ccaaaatatc gacgcataag
    12241 tgccgtttta aaaaaggaga cgggcatcgt gccgcgtctc cttttggttt gtcaaacgga
    12301 agccatgccg tctgaaaccg cctttcggag caaaaccatg atcagcattt
//
LOCUS       DAWFAD010000051        12252 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWFAD010000051 DAWFAD010000000
VERSION     DAWFAD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12252
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.051"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            26..262
                     /locus_tag="WGO49_001040"
     CDS             26..262
                     /locus_tag="WGO49_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEZ0306157.1"
                     /translation="MWIFSACSRGADPIGRPFAFVNILENLEIRSTLPLIANRPTFPQ
                     LWVNGELIGGSDIILQMYQSGELKPLIEEHSPKV"
     gene            430..2205
                     /gene="ggt"
                     /locus_tag="WGO49_001041"
     CDS             430..2205
                     /gene="ggt"
                     /locus_tag="WGO49_001041"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ0306158.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSRLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGSYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            2424..2619
                     /locus_tag="WGO49_001042"
                     /pseudo
     CDS             2424..2619
                     /locus_tag="WGO49_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2686..3936
                     /locus_tag="WGO49_001043"
     CDS             2686..3936
                     /locus_tag="WGO49_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ0306159.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLENVRKQITALCDKYPVYGA"
     gene            4027..4671
                     /locus_tag="WGO49_001044"
     CDS             4027..4671
                     /locus_tag="WGO49_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0306160.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            4690..6600
                     /locus_tag="WGO49_001045"
     CDS             4690..6600
                     /locus_tag="WGO49_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0306161.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIIQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     RVGHELENGSGEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKVQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELNPTYGRIRIGSKQEVAYFDQFRS
                     TLNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILILDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     IFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            6621..6986
                     /locus_tag="WGO49_001046"
     CDS             6621..6986
                     /locus_tag="WGO49_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306162.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            7097..8572
                     /locus_tag="WGO49_001047"
     CDS             7097..8572
                     /locus_tag="WGO49_001047"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ0306163.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVVDNLSGKIYLIVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSSLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIHAAQMVQEGLDK"
     gene            8569..9114
                     /locus_tag="WGO49_001048"
     CDS             8569..9114
                     /locus_tag="WGO49_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ0306164.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETGNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            9111..10247
                     /locus_tag="WGO49_001049"
     CDS             9111..10247
                     /locus_tag="WGO49_001049"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ0306165.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPGSDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELNYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            10294..10776
                     /locus_tag="WGO49_001050"
     CDS             10294..10776
                     /locus_tag="WGO49_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0306166.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            10882..11937
                     /locus_tag="WGO49_001051"
     CDS             10882..11937
                     /locus_tag="WGO49_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0306167.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSVVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDSFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            11934..12146
                     /locus_tag="WGO49_001052"
     CDS             11934..12146
                     /locus_tag="WGO49_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306168.1"
                     /translation="MTAMPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGALQGRITFLNHPF"
BASE COUNT         3375 a         3349 c         2790 g         2738 t
ORIGIN      
        1 tgaaaggcac gccccagttt ccccaatgtg gattttcagc gcgtgcagta gaggtgctga
       61 cccaattggc agaccctttg cttttgttaa tatcctagaa aacctagaaa ttcgcagtac
      121 tctaccactc atcgccaatc ggccaacctt tccacagctt tgggttaatg gtgagttaat
      181 cggtggtagt gatattatcc tacaaatgta ccaatcaggt gagctaaaac cactcattga
      241 agagcatagc ccaaaggtct gattacttaa tatcttaaaa taaatatcaa aaaagactgc
      301 tgatgacatt agcggtcttt ttttgatggt caagattgat agtttggatt gaattattta
      361 gccatattaa atacaataat cagcatgcct tgtatgaatc atcaatcaaa ctcaggcgaa
      421 ggagtgcttg tggctaaaac atatttattg actgcattga taatgtctat gacaatctct
      481 ggatgtcaag tcatccatgc caatcaaggt aaggttaata ctcattctgc tgtcatcaca
      541 ggtgcagacg ctcacacgcc tgaacatgca acgggactga ccgaacaaaa gcaggtgatt
      601 gcaagtgatt ttatggtagc gtcagccaat ccattagcaa cacaagctgg ctatgatatc
      661 ttaaagcaag gcggtagcgc tgcagatgcg atggtggcgg tgcagacgac actaagcttg
      721 gtagagccac agtcgtcagg cttgggcggt ggtgcatttg tgttgtattg ggataatacc
      781 gccaaaacat tgaccacatt tgatgggcgt gagacggcac cgatgcgtgc gacgccggaa
      841 ttatttttgg ataaagatgg tcaaccattg aaatttatgg aagcggtggt cggtggtcgc
      901 tcggtgggta cgcctgctat ccctaaactg atggagacga tacatcagcg atacggtgta
      961 ttgccttggg gaaaattatt tgatacgccg atccgcttgg caaaacaagg ctttgaggtg
     1021 tcgccaaggc ttgccatctc ggttgagcaa aatcagcagc atttggcacg ctatccaaaa
     1081 acagccgctt attttttgcc gaatggtgtg ccgctacaag caggcagctt gctgaaaaat
     1141 ttagaatttg ctgacagtgt tcaggcgtta gcagctcaag gtgcaaaagc tctgcatact
     1201 ggtaaatatg cccaaaatat cgtttcagtt gtccaaaatg ctaaggataa ccccggtcaa
     1261 ttatccttac aagatttatc tgattatcaa gtggtggaac gcccgcctgt ttgtgtgact
     1321 tatcgtattt atgaagtatg cggtatgggt gcaccaagct caggtgggat tgctgtgggt
     1381 cagattttgg ggattttaaa tgaattttca cccaatcagg tggggtatga tgctgaaggt
     1441 ttacgtctct tgggcgacgc ttctaggctt gcgtttgcgg atcgtgatgt atatttgggc
     1501 gaccctgatt ttgtaccagt acccattcgc cagttgattt ctaaagacta cctaaaacat
     1561 cgcagccggc tgcttgagca gtcggataag gcattgccta gcgtatctgc aggcgatttt
     1621 attcatgagt gggtgtcctc acaagcgatt gagttacctt ctaccagtca tatatcaatt
     1681 gtggataagg cgggcaatgt gctatcaatg acgacttcca ttgaaaatgc gtttggctca
     1741 actttgatgg caaatggcta cctgctgaat aatgagctga cagactttag ttttgagcca
     1801 ataaagcagg gcaaacaggt cgccaatcgt gtcgagcccg gcaagagacc acgctcctca
     1861 atggcaccaa ccatcgtatt taaagcaggc aagccttata tggcaatcgg ctccccaggt
     1921 ggtagccgta tcattggcta tgtcgctaag acgattgtgg cacatagtga ctggaatatg
     1981 gatatccaag atgccatcag tgcacccaat ttattaaatc gctttggtag ctatgaacta
     2041 gaaacgggta caaccgccct ccagtggcaa caagcattaa atgatttggg ctataagact
     2101 gacgtgcgtg agctaaattc aggtgttcaa gccattatta tcgagccaag caggctggtt
     2161 ggtggtgccg atccacgccg agaaggtcga gtgatgggtg attagaggtg aagcttgtat
     2221 tttgtgaatt tgaccgcatt aagttaatat taaaataggg ggaaattgtg agtaatcaaa
     2281 caaaaataca agccatcaag caagcttctg agcagatctt cgcaatttgt gagacgccaa
     2341 acacagcatt acaagccatt catctgattt taagacatgg tggggcagga gagttgtctt
     2401 ggcaagtggt gtatcagcgg gttatggctg ataaagatgt ggttggtgct ggttatttga
     2461 ttgattttgc acaaactgcc gaaaatttac catttgatgc attgcctttg ggtagccttg
     2521 tactaacata aaggtaatga gacgctaaaa accagcatgt taaataaatt acctgataat
     2581 gccaaagaaa atttacgcat tatggactat ctgccttagg ttttgaagta tttttttata
     2641 caaaaaacac tcatgatacc ctgattacac aaaggagccg agaacatgtt ttcaaaaagc
     2701 gttaccctcg cacaatatga ccccgatttg gcagcagcca ttgcccaaga agaccaacgc
     2761 cagcaagacc acgtcgagct gattgcctct gaaaactacg tcagctgcgc cgtaatggaa
     2821 gcgcaaggtt cccagctgac caacaaatac gccgaaggct accccggcaa acgctactac
     2881 ggcggctgcg aatacgtcga tattgtcgaa caattggcaa tcgaccgcgt caaaaaactg
     2941 ttcggcgcgc aatacgccaa cgttcaaccg cactccggct cgcaagccaa ccaagccgtg
     3001 tacgcttccg ttttaaaacc gggcgacacc attttgggta tgtctttggc gcacggcggc
     3061 cacctgaccc acggcgcgag cgttaatatt tccggcaaac tctacaatgc cgttacttat
     3121 ggtttggatg aaaacgaagt gctcgattat gccgaagtgg aacgcttggc tctcgaacac
     3181 aaacccaaaa tgattgtggc gggtgcgtct gcctacgcgt tgcaaatcga ctgggcaaaa
     3241 ttccgcgaaa tcgccgataa agtcggcgca tacctctttg tcgacatggc gcactacgcc
     3301 ggactgattg ccggtggcga atatcccaac cccgtgccat tctgcgactt cgtgaccacc
     3361 accacccaca aaaccctacg cggccctcgc ggcggcgtga ttctgtgccg cgacaatacc
     3421 cacgaaaaag cactgaactc ttccatcttc ccaagcctgc aaggcggtcc gctgatgcac
     3481 gtcatcgccg ccaaagccgt agcgtttaaa gaagcgttgc aacccgagtt caaacaatac
     3541 gcaaaacaag tgaaaatcaa tgctgccgcc atggcggaag aactggtcaa acgcggtttg
     3601 cgcatcgttt ccggccgcac cgaaagccac gttttcctcg tcgacctgca accgatgaaa
     3661 attaccggca aagccgccga agccgctttg ggcaaagcgc acatcaccgt caacaaaaac
     3721 gccattccga acgatccgga aaaacccttc gttacctccg gcatccgcat cggctccgcc
     3781 gccatgacca cacgcggttt taacgaagcc gacgcacgcg tattggcgaa cttggttgcc
     3841 gacgtattgt ctaaccccga agacgaagcg aatctggaaa acgtccgcaa acagattacc
     3901 gccctctgcg acaaataccc cgtttacggc gcgtaaactt ttttaaaaac aaatgccgtc
     3961 tgaaaatttc agacggcatt tttccaatcg gttaaaatac gcggtttgtt tttttaggaa
     4021 cgcgccgtgc ttgcctccgc catcgacttc atcctccata tcgaccaaca cctgctcgcg
     4081 ctgtcggcgc aatacggtgt gtggatttat gcgattctgt ttttgattgt tttttgcgaa
     4141 accggcctga ttgttacgcc cttactgccc ggcgattcgc tgctgtttgc cgccggcggg
     4201 attgccgcgc tgggcggtat ggatattcat ttgatggttg cattattaag ccttgccgcc
     4261 atactcggcg acgcgctcaa ttttaccgtc ggcaaatatt tcggcggcag gctgttcgcc
     4321 aaccccgatt ctaaaatctt ccgacgtgaa tatctcgata aaacccaccg tttttacgaa
     4381 aaacacggcg gcaaaaccat catcattgcc cgttttatgc ccatcgtccg cacctttgcc
     4441 cccttcgtcg ccggtatggg gaaaatgcac tatgccaaat ttatccgcta caacatcatc
     4501 ggtggcttgt tgtgggttat cctgttttct tacgcgggct atttctttgc caacttcccc
     4561 gtcgtaaaaa acaatctggg cttggtgatg ggcggcatca ttattgtttc cgtcctgccg
     4621 ggtgcggttg aaattgcccg cgccaaactt gcggtaaaat ccaaacggta actgttgaga
     4681 atttgcatca tgaatatatt atctgtagaa aacgcttctt ttgccatcgg ccacgttgcc
     4741 ttgctcgaca aaacttcttt tcaactcgac agcggcgaaa aagtcggctt gatcggacgc
     4801 aacggcgcag gtaagtcttc gtttctgaaa atcctcgccg gcgtgcaaaa gctcgacgac
     4861 gggcagatta tcattcaaaa caacctcaaa atcgtttatg taccgcagga atcctttttt
     4921 gataaggacg caaccgtatt tgataccgtt gccgaaggtt tgggtgaaat tcgcgattta
     4981 ttgcgccgtt accaccgcgt cggccatgag ttggaaaacg gttcgggtga ggctttgttg
     5041 aaagaactca acgaattaca acttgaaatc gaagcgaagg acggctggaa gctggatgcg
     5101 gcagtcaagc agactttggg ggaactcggt ttgccggaaa acgaaaaaat cggcaacctt
     5161 tccggcggtc agaaaaagcg tgtcgccttg gcgcaggctt gggtgcagaa gcccgacgta
     5221 ttgctgctgg acgaaccgac caaccatttg gatatcgacg cgattatttg gctggaaaat
     5281 ctgctcaaag cgtttgaagg cagcttggtt gtgattaccc acgaccgccg ttttttggac
     5341 aatatcgcca cgcggattgt cgaactcgat cgcggtattt tgcgttccta tcccggctcg
     5401 ttctccaaat acagcgaaaa aaaagtgcag gaattggcgg ttgaagcgga acacaaccgc
     5461 ctgtttgaca aattccacgc gcaggaagaa gcgtggatac gcaaaggcat cgaagcgcgc
     5521 cgcacccgca acgaaggccg cgtgcgccgt ttggaagagc tgcgccgcca acgcgccgaa
     5581 cgccgcaatg tgcagggaca ggtcaacttc aagctcgaca gcggcgagaa aagcggcaaa
     5641 atcatcgccg aattggaaca tgcctcgttt gcctatggcg acaaagtcat tatggacaaa
     5701 ttctccgcga ttttgcagcg cggcgataaa atcggtctaa tcggccccaa cggcatcggc
     5761 aaaaccacat ttttaaaact gattttaggc gaactcaacc cgacttacgg cagaatccgt
     5821 atcggcagta agcaggaagt tgcctatttc gaccaattcc gcagcacgtt gaacgaaaac
     5881 gacaccgtgt tttacacgct cggtcaaggc aacgattacg tcgaagtcgg cggcaagaaa
     5941 aaacacgtaa tgagctatct ggaagatttc ttattccatc cagcccgcgc acaaagtccc
     6001 gtttcatcgc tctccggcgg cgaacgcaac cgccttttgc tggcaaaact ctttacccgt
     6061 cctgctaata ttttgatttt ggacgaaccg accaacgact tggatatcga tacccaagag
     6121 ctgcttgaag atttgctgcg cgattatcaa ggcacggtat tccttgtctc acacgaccgt
     6181 atgttcctcg ataacgtgat tacccaaagc attatttttg aaggaaatgg aaggttaaaa
     6241 gaatatatcg gcggctatca ggactacatc gacgcaaaat cacgcgaagc gaagattcag
     6301 acggaatccg cacccaaaac agccgatgcc gaaccggtga aagaaaaacc caaagccaac
     6361 cgcacggtca aactgtccta caaagaacag cgcgaactcg acgccctgcc cgacgaaatc
     6421 gccgccttgg aagccgaaca ggcagaaatc aacgcgcagc tttccgatcc cgaaatcttc
     6481 aaagattacg aaaaagccgg cgcactgcaa agccgcgccg aagaaatcga aatgctgctt
     6541 ttggaaaagc tggaacgctg ggagctgctg gaaaccaaac agaacggcaa tgccgtctga
     6601 aatcaaacag gaagaaaaaa atgttcaaac gacctgaaga aatcattgtc ctgatacttg
     6661 ccgtcctctg gattgccggt acgtattttc tcgccgccct gtttggagcg gatgcttata
     6721 ccgtgttaaa aatcactgcc cttaccctgc tttggtctgc cgcatcattc ctcctttggc
     6781 aaaaaaacct cagccggcat atctggccgc tgctgctcgg ctgcctgact gcctgttggt
     6841 ggccgtatct gaatcaatcg gtcgcacccg gttctttacc ttggtatgca tcatggacgt
     6901 tcaaaaccat cttagccctg attcccgtaa ccgcaggtta tctgtttaaa tggaaacaat
     6961 accggcagcg caaaacaaag ccgtaagccc gtccttttca gacggcatct ccggtcagac
     7021 tgtcaacaat tccgataaaa tgctatactc gcgcccgttg ccgcgccgtc cgaaaccgcc
     7081 ctttgggaaa gcagaaatga tcagcaaaca agaataccaa gcccaagccg ctcaaggcta
     7141 caaccgcatc ccgctcgtgc aagaactcct tgccgatttg gatacgccgc tttccctcta
     7201 tctcaaactc gccaaccgcc cctataccta cctgctcgaa tccgttgtcg gtggcgaacg
     7261 tttcggccgc tattctttta tcggcctgcc ttgcagccac tatctcaaag ccagcggcaa
     7321 acacgtcgat gtttatcaaa acggcgaaat tgtcgagcaa cacgacggca accccctgcc
     7381 ctttatcgaa gccttccaca accgcttcaa aacacccgaa atcccaagcc tgccgcgctt
     7441 taccggcgga ctggtcggct acttcggtta cgaaaccatc tacaattttg aacacttcgc
     7501 ccaccgcctg aaaaacacca ccaaagccga cccgctcggc acgcccgaca tcctcctgat
     7561 gctctcgcaa gagcttgcag tagtggataa cctaagcggt aaaatctacc tgattgttta
     7621 cgccgatccg tcgcagcccg acggctacga acgcgcacgc gaacgcctcg aagacatccg
     7681 tacacagtta cgccaaagtt gcgccatccc gctttcgctc ggcagcaaac acaccgaagc
     7741 cgtcagcgaa ttcggcgaag agccgttcaa agcctgcgtt aacaaaatca aagactacat
     7801 ctttgcaggc gactgcatgc aggttgtccc cagccagcgc atgagcatgg aattcaccga
     7861 cagctcgctc gccctctacc gcgccttgcg cacgctcaac ccttcgcctt atctcttcta
     7921 ctacgatttc ggcgatttcc acatcgtcgg ctcctcgccc gaaatcctcg tccgccgcga
     7981 acgcgacgat gtcatcgtcc gccccatcgc cggcacgcgc ctgcgcggca aaacccccgc
     8041 cgaagacctt gccaacgaac aagatttgtt aagcgatgcc aaagaaatcg ccgaacacgt
     8101 catgttgatt gacttaggac gcaacgacgt cggccgcatc agtaaaaccg gcgaagtcaa
     8161 agttaccgac aaaatggtga ttgaaaaata ctcccatgta atgcacatcg tttccaacgt
     8221 cgaaggccgt ctgaaagacg gcatgaccaa catggacatc ctcgccgcta ccttccctgc
     8281 cggcacactc tccggcgcac ccaaagtccg cgccatggaa atcatcgaag aagtcgaacc
     8341 aagcaaacgc ggcatctacg gcggtgccgt cggcgtatgg ggtttcaaca acgacatgga
     8401 tttggcaatc gccatccgca ccgccgtagt aaaaaacaac acgctatacg tccaaagcgg
     8461 cgcgggcgtg gttgccgact ccgatccggc ctccgaatgg caggaaacac aaaacaaagc
     8521 ccgagccgtc atacacgccg cgcagatggt gcaggaaggg ttggataaat gacttatgaa
     8581 aaactttatg agcttcgcca aacactcaga ccgactacca aagacggtct ctatacagat
     8641 aatgaaaaaa gtcgagaaat cctaacggtt agatggagcg gcaacacaga aaatcctaaa
     8701 aatttctcag ctgttgctat aggaattaat ccaagtaaag caaatgatga aagaagcgac
     8761 aaaaccctta cccaacttgc acgtttcttg gatatgtacg gttttaccaa cttcaaaatg
     8821 ttaaatattt tttccagtta ctctacacag caaacaggaa ttagggcaaa cacccaaacc
     8881 gatttttcca aatttaaagg atgtttggaa gatgctgata tgattattct tgcatggggt
     8941 acagaccgcg gtgcttataa ggatgaaaaa aacagaatac tggaatttct aaaagcagaa
     9001 aaatttatgg aaaaagtatt ctgtatttct gaaactggta attccagcga tacaagacat
     9061 ccgtcaagaa ttagttatag ctatcaactt gtacaatttg aagagtcagc atgaaaaaca
     9121 tatctctttc tccgcccgcc atgcttggca tcctcggcgg cggacaatta ggcagaatgt
     9181 ttaccgttgc cgccaaaacc atgggctaca aagtaaccgt tctcgacccc gatccggacg
     9241 cgccggcagc agaatttgcc gactgtcatt tatgcgcgcc gtttaacgac caagctgctt
     9301 tggacgaatt ggcaaaatgc gcggcggtga ccactgaatt tgaaaacgtc aatgccgatg
     9361 cgatgcgttt tctcgccaaa cacaccaatg tttcccccgg cagcgactgc gttgccatcg
     9421 cgcaaaaccg cattcaggaa aaggcatgga tacgcaaagc aggattgcaa accgcgccgt
     9481 atcaagcaat ttgcaaagcc gaagacatca ctgaagaaag catacaattt ctgcccggca
     9541 tcctgaaaac cgctacattg ggctatgacg gcaaaggcca aatccgcgtc aaaacggtgg
     9601 atgaactcaa agccgcgttt gccgaacacc gcggcgtgga ttgcgttttg gaaaaaatgg
     9661 tggacttgcg cggcgaaatt tccgttatcg tatgccgtct gaacaatgac aacgtgcaaa
     9721 ctttcgatcc tgccgaaaac attcacgaaa acggtatcct cgcctactcc atcgtcccag
     9781 cccgactgag tgccgacatt cagcaacagg cgcgacaaat ggcgcagcgt ttggccgatg
     9841 aattgaacta cgtcggcgta ttggcggtag aaatgtttgt tgtcggcgac acgcatgaat
     9901 tggtcgtcaa cgaaatcgcg ccgcgtccgc acaattccgg ccaccatacc gtcgacgcct
     9961 gcgcggcaga ccaattccag caacaggtcc gcctgatgtg caacctgcca cctgctgaca
    10021 ccaaattgct gagttcttgc tgtatggcga atattttggg cgacgtttgg caggaagacg
    10081 gcggcgaacc ggattggttt cccctgcaaa gccggccgga cgcgcacctg cacctttacg
    10141 gcaaaaaaac cgcgcacaaa gggcggaaaa tgggacactt taccgtttta accaccgatt
    10201 cggacaccgc atttcaagaa gcaaaaaaac tgcatcagtc cctataatcc gaccaacggc
    10261 acagatgccg tctgaagccg ccgacatact gccatgaacc tgctgacact gttacgcccc
    10321 gccaccgtgc aagactgcaa agacatcttc aaagtccacc tgcactccgt ccaatacacc
    10381 tgcatcctca gctacaacga acacgcccta aaagtatggg aaggcttgtt aaacacagaa
    10441 agctacctgc cgaccatttc cgatcccgac aaagcacttt gggttgccga atacaaaggc
    10501 aacatacagg gatttttcca aatcgactgc caagaagcac aactcgatgc actttacgtc
    10561 caccccctgt tccacaacct cggactgggc actgccctgc ttcatcaggc ggaaaccatc
    10621 gcccacaaat ccggtttgag ctttctgaaa ctctacgcat cgctcaactc cgtcccgttt
    10681 taccttctca accgctacga atcgctcggt tcggcagtat tgcagcttga ccccagcatc
    10741 aaaatcaaat gcgaactgat gcggaaacac ctctgatccg tccttataac caaaactgat
    10801 aatcttcaaa tcaaacatta tttccacaag cgcagacagc cgtataaagt tgccccatct
    10861 accaaccgac aggacaagca tatgaaaacc tacgcaccgg cactctatac cgcagccctg
    10921 ctcaccgcct gcagccccgc agccgattca aaccatccgt ccggacaaaa tgccccggcc
    10981 aataccgaat ccgacggaaa aaacattacc ctgctcaacg cctcatacga tgtggcacgg
    11041 gatttttaca aagaatacaa ccccttattt atcaaaacat accaatccga acaccccggc
    11101 acatccgtca gcatccaaca gtcccacggc ggctccagca aacaggcatt atccgtagcc
    11161 aacggccttc aagccgatgt cgtaaccatg aaccaatcct ccgacatcga cctgctcgaa
    11221 aaaaaaggac tggtagaaaa aggctggcaa caagccctcc ccgaccacgc tgcgccctac
    11281 accagcacta tggttttcct tgtccgaaaa aacaacccca aacagatccg cgattggaac
    11341 gaccttgcca aagacggcgt taacatcgtc atcgccaatc ccaaaacctc gggcaacgga
    11401 cgctacgcct tcctcggcgc atacggttac ggtctgaaaa ccaccaacgg caacgaacag
    11461 gaagcccaaa aactcgtcgc atccatcctc aaaaacaccc ccgtttttga aaacggcgga
    11521 cgcgccgcca ccaccacctt cacacaacgc aacatcggcg acgtactcat tacttttgaa
    11581 aacgaagcca actacgtcag caaaaaactg acccaaggac agtttgaaat cgtctatccg
    11641 agctacacca tttccgccga aagccccgtc gccgtcgtca acagcgtcgt cgcgaaaaaa
    11701 ggcacgcaaa aaaccgcccg cgcctatctc gaataccttt ggagcgaacc ggcgcaagaa
    11761 ctcgccgcct cactctacct gcgcccccgc aaccccgaag tattggcaag acacaaagcc
    11821 gacttcccag acttagactc attctctccc gaagaaaaat tcggcggatg ggacaacatc
    11881 atgaaaacct acttcgccga cggcggcata ttcgaccgcc tgaccgcaca aaaatgaccg
    11941 ctatgccgtc tgaaccgcct ttcagacggc attccacaca tcaaaacaga aaaaccgcgc
    12001 ccaaaatcat catcacaaga cacaacggca aaccggatac aaaacccaac accgccgcca
    12061 aaatgccgtc tgaagccgtc ggcgcaacca tccttatctg gggcgcgctt caggggcgca
    12121 taactttcct taaccatccc ttttgacagc caaataaaca gtgcttttgc ctgtcagaaa
    12181 caaaaccaat attttgcagt tcgtatcgga acatttatag tggattaaca aaaaccagta
    12241 cagcgttctc gc
//
LOCUS       DAWFAD010000052        11760 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWFAD010000052 DAWFAD010000000
VERSION     DAWFAD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.052"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            97..813
                     /locus_tag="WGO49_001053"
     CDS             97..813
                     /locus_tag="WGO49_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0306169.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="WGO49_001054"
     CDS             962..1864
                     /locus_tag="WGO49_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ0306170.1"
                     /translation="MDSLMTLLSVLVPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFAFGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSTLG"
     gene            1907..2680
                     /locus_tag="WGO49_001055"
     CDS             1907..2680
                     /locus_tag="WGO49_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ0306171.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEAGSLKRHHGGASSGGNLPEGLPADRQTRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="WGO49_001056"
     CDS             2792..4480
                     /locus_tag="WGO49_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ0306172.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="WGO49_001057"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="WGO49_001057"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ0306173.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSGELSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQAMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            5841..6224
                     /locus_tag="WGO49_001058"
     CDS             5841..6224
                     /locus_tag="WGO49_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ0306174.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            6339..6812
                     /locus_tag="WGO49_001059"
     CDS             6339..6812
                     /locus_tag="WGO49_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ0306175.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMSAMDHGHHHGEAHQH"
     gene            6938..8041
                     /gene="mnmA"
                     /locus_tag="WGO49_001060"
     CDS             6938..8041
                     /gene="mnmA"
                     /locus_tag="WGO49_001060"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ0306176.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFNLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQTTDKPVIITR"
     gene            8112..9782
                     /locus_tag="WGO49_001061"
     CDS             8112..9782
                     /locus_tag="WGO49_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ0306177.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSISDVFRQSVEKFARLPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMMPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGTTAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRINVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVRKDPS
                     LTKEELIAFCRTELTAYKVPKDIEFRDELPKSNVGKILRRELRQSTGK"
     gene            9894..11528
                     /locus_tag="WGO49_001062"
     CDS             9894..11528
                     /locus_tag="WGO49_001062"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ0306178.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSEHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         2911 a         3175 c         3113 g         2561 t
ORIGIN      
        1 atttgtgcaa tgctttaatt cctctgacac gatttgaaac acagccgcct taaaacatct
       61 taaaaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccctgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc cggcaaaaag
      241 ctgaccgaat ccgaactttg ccgtcagatg gtgtgttcgc gcaacaccgt ccgcggcgcg
      301 ctgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc agatttgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgataa ggcgcgaaga agaggcttcc ggcaggggcg accgcgtcgg ctggaaccgc
      541 ctgtccaatg ccttccacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggaacgct tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca tatatcatgc ggcaataaag gaggtcagga
      961 gatggacagc ctgatgacat tgctttcggt attggtaccg atgttcgccg gattttttat
     1021 ccgtgtgccc aagccttacc tgcccgcttt ggacaaggtg ctgtcggttt tggtgtatgc
     1081 cgtgctgctg ctgatcggcg tatcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcggggcga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggcgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcattcgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaagcgcg ggcatgtatt gtctgatgct
     1381 gctggtgttc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcgggcgg
     1501 gctgctgttt gccgcatcga cagacggtgt gtcgtggacg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtcatgacc gaggcttacg gcgcggtatg
     1621 gggcagcatc atgctgctga acgatttggc acgagagctg tttgcactgg catttatccc
     1681 gctgctgatg aagcgttttc cggatgcggc ggtgggggtt ggcggtgcga ccagtatgga
     1741 ttttacattg cccgtgattc agggtgcggg cggtttggaa gtcgtgccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata ttgccgcccc gtttctgatg gtggtgtttt ccacgctggg
     1861 ctgaacacgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgagttgga
     2041 ggaggccggc agcctgaaac gccatcacgg cggcgcatct tcgggcggaa acttgccgga
     2101 gggcctgccc gccgaccgcc aaacccggtg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa cacatccccg acggttcgtc gctgtttgtc agtatcggca cgacgatgga
     2221 ggccgtggtg tcggagctgg tcaggcggcg cggcagcctg agggtgatta ccaacaatat
     2281 ccacgccgcc tcgattgctt cggcgcggac ggattatacg gtcattatca cttctggagt
     2341 cgtccgtcct ttggacggcg gtattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaatgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgcc tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat ttgcggacaa
     2821 ccattttatc cgcaccttca tcgaagaaga cctcaaaagc ggcaaacacg aaaccatcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggcgt atatttacga cggcttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagacg ttgagtggct
     3061 cggtttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttggtta aagacggcaa agcgtatgtc gatgatttga cgccagaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcaggcaaa aacagccctt atcgcgaccg
     3241 cagtatcgaa gaaaacctcg acctgttcac gcgcatgaaa aacggtgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatccggc aacatcaata tgcgcgatcc
     3361 ggtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgcatcta
     3421 tccgatgtac gactacacgc attgtatttc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctc gaatttgaag cccatcgtcc gctgtatgac tgggtgttgg acaacatccc
     3541 cgcgccgcac gccacacgtc cgcgccaata cgagttttca cgcttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacca tttccggtat gcgccgacgc ggctacacgc ccgaagggct
     3721 gcgcctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaattccgcc ccacgcctga tggcggtgtt
     3841 gaacccgctc aaagtgaccc tgaccaactt tgaaaccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aggaaatggg cgagcgcgaa gtacctattt cacaaaccat
     3961 ctatattgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtggttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tccagaaggc cgtaaagtta aaggcgtgat tcattgggtt tccgccgaac acgccgccga
     4201 aatcaaagtc cgtctgtacg accgcctctt taccgtcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagacggc gaatacctgc cgttcaccga tttcctcaat ccggaatccg tcaaggaaat
     4321 caccgcctac gccgaacctg ccgccaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgtaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ttgccgtctg
     4501 aatattgttc ggacggcatt tcccctttgc ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctac
     4621 aaagacacca cctacgccat gatgcgcgag atggcaaaac gcggttggcg gctgtttcat
     4681 accttgagcg gggaattgtc ggttaaaaac ggtctggtgg ttgctgatgc gtctgctttt
     4741 gagtttctcg gcgcacaaga cgaccacgac aaaaactggt ttgccgccgc cgacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccacaca gcttctgacg ctggcggagc agcagggcgc gaaggtcttt
     4921 aacagcggac aggcgatgcg tgatttcaat gaaaaactgg cgattttgaa cttcagccgc
     4981 tttaccgcgc ccacgctggt aacgacccgt tccgccgatg tccgcgcatt tttgaaagaa
     5041 cacggcgaca tcatcattaa accgctggac ggtatgggcg gtatgggtat tttccgcctg
     5101 accgaaaaag atcccaacat cggcagcatc ctcgaaaccc tgatgcggct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtatcc cgcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg acgcggcgtg gcgcaggaat tggacggacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgctggc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatt
     5461 atgaaacaaa aaggtttcga cgtggcggca atgtttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgccgt ttgaaaggct tttgcttcac aaccgtttgg tttgttcggg
     5581 caggcagggt tttccctcag ccggcaggga tgcggttaat gccgtacgga caccccgccg
     5641 ccccgttttc agacggtata tatagtggat taacaaaaat caggacaagg cggcgaagct
     5701 gcagacagta caaatagtac ggaaccgatt cactcggtgc ttcagcacct tagagaatcg
     5761 ttctctttga gctaaggcgg ggcaacgccg tacaggtttt tgttaatcca ctatattgag
     5821 aacattttga aaggataccg atggaacctt cctcctacgc ggcagaaaaa aaaggcaaag
     5881 gcggcatcag gcgcgtcatt aacgcattcg gctattcgat agacggcatc gccgccgcct
     5941 accgttacga agcggcattc cgtcaggttt tgtggctgaa cgtactgctg gtgtgcgcgg
     6001 catttttttg ggtttccgaa accgccgtcc gcctgccgtt gattatcgcg tcttttgtgt
     6061 cggtcattgt cgaactgttc aacaccgccg tcgaagctgc cgtcgatcat acttcgaccg
     6121 aaaaacacga gctggccaaa cgcgccaaag acgcaggttc tgccgcacaa ttgttcgcga
     6181 tgctgatgtt ggcggcggtt tggctgtctg ccctgttcgg gtaaaacgct tgcagcagga
     6241 tttgtaatcc tttaggatta gtatgtggta tttattattc acttaatcca ctataagatt
     6301 aagcacattc cccgtctgat attataagga gcagaaagat gaaaaaatta ttggcggccg
     6361 tgatgatggc aggtttggca ggcgcggttt ccgccgccgg agtccacgtt gaggacggct
     6421 gggcgcgcac caccgtcgaa ggtatgaaaa taggcggcgc gttcatgaaa atccacaacg
     6481 acgaagccaa acaagacttt ttgctcggcg gaagcagccc cgttgccgac cgcgtcgaag
     6541 tgcataccca catcaacgac aacggcgtga tgcggatgcg cgaagtcaaa ggcggcgtgc
     6601 ctttggaggc gaaatccgtt accgaactca agcccggcag ctatcacgtg atgtttatgg
     6661 gtttgaaaaa acaattaaaa gagggcgaca agattcccgt taccctgaaa tttaaaaacg
     6721 ccaaagcgca aaccgtccaa ctggaagtca aaatcgcgcc gatgtcggca atggaccacg
     6781 gtcatcacca cggcgaagcg catcagcact aatctgctga aaatatttga aatgccgtct
     6841 gaaaaagccc gggctttcag acggcatttt tatgaccgcc tttaaaatgt gttaaaatct
     6901 gcctttaaaa accgccgttt ccaaccatat tctacgcatg aatacgacag caaacccttc
     6961 caatatcatc gtcgggctct ccggcggtgt cgattcttcc gtaaccgccg ccctgctcaa
     7021 gcagcagggt tatcaagtgc gcggtgtgtt catgcagaac tgggaagacg acgacaacga
     7081 cgaatattgc agcatcaaac aggattcgtt cgatgccatc gccgttgccg atattatcgg
     7141 catcgacatc gacatcgtta atttcgccgc gcaatataaa gacaaagtct ttgcttattt
     7201 tcttcaggaa tacagtgcgg ggcgcacgcc gaatcccgat gtgttgtgca acgccgaaat
     7261 caaattcaaa tgctttttgg attacgccgt agggcagggc gcggatacca tcgccaccgg
     7321 acactatgcg cgcaaagaag tccgcaacgg cgtgcattac ctgctcaaag gtttggatcg
     7381 aaacaaagac caaagctatt tcctctaccg cctcaagcct ttccaactcg agcgcgcgat
     7441 ttttccgttg ggcggtttgg aaaaacctga agtccgccgc cttgccgccg aatttaattt
     7501 gccgaccgcc gccaaaaaag acagcacggg catctgcttc atcggcgaac gcccgtttcg
     7561 cgagtttctg caaaaatacc tgccgaccga caacggtaaa atggttacgc ccgaagggaa
     7621 aaccgtcggc gaacacgtcg ggctgatgtt ctacacgctc ggacagcgca aaggtttggg
     7681 catcggcggc gcgggcgaac cgtggtttgt tgcggctaaa gatttgacga aaaacgaact
     7741 gattgtcgtg caaggacacg accatccgct gctctatacc cgcagccttg tgatgaacga
     7801 tttgagtttc acgctgcccg aacgcccgaa ggcaggacgc tatacctgca aaacgcgtta
     7861 ccgtatggcg gacgcgcctt gtgagttgcg ctatttggat gaagaaaccg ccgagctggt
     7921 gtttgacgaa ccgcaatggg cggttacgcc gggccagtct gccgtgctgt acgacggcga
     7981 tatctgtttg ggcggcggca tcatccaaac gaccgacaaa cccgtcatca tcacgcgata
     8041 aaggtaatgc cgtctgaaac ggttttcaga cggcattgtt ccgctcaatt ccactttaaa
     8101 gaccgatacc tatggaaaaa atctggttag acagttacga gaagggcgtc agtgccgaaa
     8161 tcgatatcac gcaatacaat tccatcagcg atgtattccg ccaaagcgtg gaaaaatttg
     8221 ccaggctgcc cgcttttcaa aatatgggca aaacgctgac ttatgccgaa accggcaaac
     8281 tggcgactga tttcgcctct tatctgcaaa acgtcctcaa gctgccgcgc ggcgaacgtg
     8341 ttgccattat gatgccgaac gtattgcaat acccgatagc ccttttcggc attttgcagg
     8401 caggtttggt ggcggtgaac accaatccgc tctatacgcc gcgcgagctg gagcatcagc
     8461 tgaaagacag cggcacgacc gccatcatcg ttttggaaaa tttcgccaac acgctggagc
     8521 tggtgctgcc gcgcacgcag atcaaacatg tcatcgtcgc ctccgtcggc gaaatgttcg
     8581 ggctgcttaa aggttcgctg atcaatttca tcatccgaaa aatcaagaaa atggttcccg
     8641 aataccgtat tcgggaaacc gtttcctttc agacggcatt gaaagagggg gcgaagcacg
     8701 ttttccaacc tgtcgcatta aaccgcgaag ataccgcgct gttgcaatac acgggcggca
     8761 cgacaggtgt tgccaaaggg gcggtgctga gtcacggcaa catctgtgcc aatatgcttc
     8821 aggcaaaaga atggattaaa aaccagttgc gcgagggcaa agaaaccgtt atcgctgccc
     8881 tgccgctgta ccatattttc gccttaaccg tgaatctgat gatttttgcc aatgccggct
     8941 caaaaatcat cctgattacc aacccgcgtg atatgaaagg ctttatcgga gaactgaaaa
     9001 aacagcggat taatgtattt atcggcgtaa acacactgtt taacgcgatg gttaaccgtc
     9061 ctgattttgc cgaagtcgat ttttcagaat tgcggctgac tttgggcggc ggtatggcga
     9121 cccaaaaagc cgttgccgaa aaatggaaaa aaattaccgg tacgcctatt gtcgaagcct
     9181 acggtctgac cgaagccagc ccaggcgtgt gctgcaaccc cttaaacatc gaatcataca
     9241 gcggcagcat cggtttgccc gtctcgtcca ccgaagtcga actgcgcgac gcaaacggca
     9301 aagaagtccc cgtcgggcag ccgggcgaat tgtgggtaaa aggccctcaa gtgatgcaag
     9361 gctactggaa ccgtcccgaa gaaaccgcta aagccataga cgcgcgcggt ttcttggaaa
     9421 ccggcgatat tgccgtcatg gacgaaaaag gctggttgaa gctggttgac cgtaagaaag
     9481 acctcattgt tgtttccgga ttcaatgttt atccgaacga aatcgaagaa gtcatcgcgc
     9541 atcacggcaa agtgatggaa gttgcctgta tcggcgttcc cgacgaaaaa accggcgagg
     9601 cactcaaagt attcgtcgtc agaaaagacc cgtctttgac gaaagaagaa cttatcgcct
     9661 tttgccgcac cgaattaacc gcatataaag taccgaaaga tatcgaattc cgcgacgagt
     9721 tgcccaagtc caacgtcggc aaaatcctgc gccgcgagtt gcggcaaagt accgggaaat
     9781 aaagaaaaga tgccgtctga aaacagccgt ccaccgttca gacggcatcc gtccgtttgc
     9841 cagaaccgcg cgcttcacgt taaaatcacg cattccaata tgggtattcc atcatgacca
     9901 aattcatttt cgtaaccggc ggcgttgtct cctcactggg taaaggtatc gccgccgctt
     9961 ctattgccgc catcctcgaa tcgcgcggct tgaacgttac catgctcaag ctcgatcctt
    10021 atatcaacgt cgatcccggc acgatgagcc cgttccaaca cggcgaagtg ttcgtaaccg
    10081 acgacggcgc ggaaaccgac ctcgacttgg gacactacga acgctttatc gattccacga
    10141 tgacccgccg caacagcttc agcacggggc aggtgtacga aaacgtcatc gccaaagaac
    10201 gacggggcga ctatttgggc ggcacggttc aagtcattcc gcacattacc gacgaaatca
    10261 aacgccgcat ccacgaaggc gcggcaggtt acgatgtggc gattgtcgaa atcggcggta
    10321 cagtcggcga tatcgaatct ttgccattct tggaagccat ccgccagatg cgaagccagt
    10381 tgggacgcaa caacaccctg ttcgcccact tgagctacgt cccctacatc gccgctgcag
    10441 gcgaaatcaa aaccaagccg acccagcaca ccgttaaaga aatgttgagc atcggtttgc
    10501 aacccgacat cctgatttgc cgtatggaca ggacaatgcc tgcggacgaa cgccgcaaaa
    10561 tcgccttgtt ctgcaacgtg gaagaacgcg cgattgtcgg cagctacgat gtggacagca
    10621 tctacgaatg ccccgaaatg ctgcacgacc aaggcatcga caacatcatt accgagcagt
    10681 tgcagcttaa tgtgcagcag gcggatttga ccgcgtggaa aaaaatcgtc cacgccatcc
    10741 aaaacccgaa acacaccgtc aaaatcgcca tggtcggcaa atacgtcgat ttgaccgagt
    10801 cctacaaatc gctgatcgaa gccttgaaac acgcaggcat tcacaccgaa accgacgtac
    10861 aaattacctt tgttgacagc gaaagcatcg agaaaaacaa cggcgacgtt tccatgctta
    10921 aagacatgga tgccatcctc gttcccggcg gttttggttc gcgcggcgtg gaaggcaaaa
    10981 tcgccgctgt gcgctatgcc cgcgaaaaca acgtgccata cttgggcatc tgcctcggta
    11041 tgcagattgc gctgatcgaa tacgcccgcg acgtggcagg tttgaaaggt gcgaattcca
    11101 ccgagttcga cctcaaatgc gccgcccccg tcgttgccct gattgacgaa tggcaaactg
    11161 ccgacggcag cgtcgaaacc cgcgacgaat ccgccgattt gggcggcacc atgcgtttgg
    11221 gcgcgcaaga agtcgaattg aaagcaggca gcctcgccgc caaaatctac ggcagcgaac
    11281 acatccgcga acgccaccgc caccgctacg aagtcaacaa caactacgtt cctacgctgg
    11341 aacaggcagg tttggtcatc ggcggcgtat ccgccggacg cgaacgcttg gtcgaaacca
    11401 tcgaactgcc gaaccatcct tggttcttcg cctgccagtt ccaccccgag tttacctcca
    11461 atccgcgcaa agggcatcct ttgttcaccg cgtttgtcaa agctgcgttg aacaataaaa
    11521 aagcctgata aagcgttact tgatgatcaa aatgccgtct gaaagcctga atgaagactt
    11581 tcagacggca tttttgcaaa tcggggatca taaaagccat caatttaatt tttatggttt
    11641 tatctaatat tgaccaactg aatattgtta agcggggcgt gaaactcaag ttgagacctt
    11701 tgcaaaattc cccaaaatcc cctaaattcc caccaagaca tttaggagca ccttcttccg
//
LOCUS       DAWFAD010000053        11611 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWFAD010000053 DAWFAD010000000
VERSION     DAWFAD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.053"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(227..1270)
                     /locus_tag="WGO49_001063"
     CDS             complement(227..1270)
                     /locus_tag="WGO49_001063"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ0306179.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIWDIPTWEHWYGMPHAAPGLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(1267..1548)
                     /locus_tag="WGO49_001064"
     CDS             complement(1267..1548)
                     /locus_tag="WGO49_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306180.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPAGFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQRSDGIPANHTKEKP"
     gene            1857..2957
                     /locus_tag="WGO49_001065"
     CDS             1857..2957
                     /locus_tag="WGO49_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0306181.1"
                     /translation="MYQKPKLFKGAVRNLEAASWKDINEIYQRADPTAPLFYLRKKGA
                     IVPKEEYVESFDALGKTRYRFIKSVIYEHMKNGASLVYNHINNEPFSDHISRQVARLA
                     GAHTIVSGYLAFGSDESYKNHWDTRDVYAIQLFGKKRWQLTAPDFPMPLYMQQTKDTD
                     ISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHLAVGTFPPNGYNYLEWLMKK
                     FPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPVNYEAFSEDFLGKERTDTAFHLE
                     QFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISVDELGKKVLEHIGKNEPLL
                     LKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3186..3539
                     /locus_tag="WGO49_001066"
     CDS             3186..3539
                     /locus_tag="WGO49_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306182.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRDWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            3600..4226
                     /locus_tag="WGO49_001067"
     CDS             3600..4226
                     /locus_tag="WGO49_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ0306183.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTAEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQKLH
                     ISQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRATNLFIS"
     gene            4178..4378
                     /locus_tag="WGO49_001068"
     CDS             4178..4378
                     /locus_tag="WGO49_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306184.1"
                     /translation="MVSSDLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADI
                     LHEKRRLYEWVLELIYSMSGKL"
     gene            4687..4989
                     /locus_tag="WGO49_001069"
     CDS             4687..4989
                     /locus_tag="WGO49_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306185.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            5273..5512
                     /locus_tag="WGO49_001070"
     CDS             5273..5512
                     /locus_tag="WGO49_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0306186.1"
                     /translation="MPHLQNLSLGLKKKLPVILQTEISECGLACLAAVAGFHGFHTNL
                     RALRSKYCPRPLQNSPKSPKCLGGNFGEFCKGLIL"
     gene            5573..7315
                     /gene="hrpB"
                     /locus_tag="WGO49_001071"
     CDS             5573..7315
                     /gene="hrpB"
                     /locus_tag="WGO49_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ0306187.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISTFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGDILPGTSRMKIITAGLDAAAPFILGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGTQLMGAVVGFRGGHKVGGMFAYDLFAGKPLHKPKGFQ
                     TTNTVYGFNLNYSF"
     gene            7424..>11611
                     /locus_tag="WGO49_001072"
     CDS             7424..>11611
                     /locus_tag="WGO49_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0306188.1"
                     /translation="MNKGLHRIIFSKKHSTMVAVAETANSQGKGKQAGSSVSVSLKTS
                     GDLCGKLKTTLKTLVCSLVSLSMVLPAHAQITTDKSAPKNQQVVILKTNTGAPLVNIQ
                     TPNGRGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGAPQIGKDGALTGFDVRQGTLTVGA
                     AGWNDKGGADYTGVLARAVALQGKLQGKNLAVSTGPQKVDYASGEISAGTAAGTKPTI
                     ALDTTALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADG
                     TEASPTYLSIETTEKGAAGTFISNGGRIESKGLLVIETGEDISLRNGAVVQNNGSRPA
                     TTVLNAGHNLVIESKTNVNNAKGSANLSAGGRTTINDATIQAGSSVYSSTKGDTELGE
                     NTRIIAENVTVLSNGSIGSAAVIEAKDTAHIESGKPLSLETSTVASNIRLNNGNIKGG
                     KQVVLMADGDIQAKASNLNASGNLYIHAGKDLDLNADKDLSTQSISLRADNTALISSN
                     GNTLTAEKNLDIQAGSLSVRQSNLQSSGGNVQMSATKGNISLNQSWINASQNIDTAAL
                     QGNIISDGLTAVAEVGRVSLLANGNVDFNGLNTLIAEGDINAGSVGKGRLKMDNTDIY
                     ASAGDVKLVAGGQLDLGNGTVNGGHISLDSNKGSMVVQDVHLNARASLKVDADQTLTI
                     NNSKLNSGHNTQINTNHGHMTLNQLDAHSRRHMSISAQGKGKGKDSGQILQNDQQNSK
                     STLAADGVLSLNSSALQVLDNTTLRGGAINIKAGGGIIKRGYIDWETQDTATMRSAEL
                     KPLSGMMSIESGGDNPLTVEPGNRIVSAGDLAVNHNGTFQISARAGNNGNPSAQTASV
                     SAKGNIGIVAGEVDIDAANIAAGKDLALVATKGNISLNSIRNTFSNYQLKTDKHNITQ
                     QLTDVEQELSKLTSDPKYRKAQDLPQMLRRKYKRRDKVFGDSEARLRGLRAEINAADE
                     AWAELQSPVKALLERKQLLQQALLTVSQPGSGHENQGSTLSGQNIKLLAAGGIRIQGS
                     KVAATQQANIQAAGFLPAPAAEELQEGRLQSAIDISGVFDTFEYGQQGSDKYGYAIFS
                     RPSEISGKTGVTLSAPNANENSRISLSAANIEAENGKIKLQSYGDQSYYAGQGELYTL
                     ERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQGIDIKSGGNIDAYATAFDAPKG
                     SINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYSKAHDTTTQVMKTALPSRVVAE
                     SANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARADAKIILEGIKSSIHTETVSSSK
                     STLWQKQAGRGSNIETLQ"
BASE COUNT         3319 a         2648 c         2845 g         2799 t
ORIGIN      
        1 aatgactgaa accggagaaa tgacgaaatt gaagcgtgtg gaaatgtgtc ggaaatcgct
       61 gaagccggat aaactagatt cccgcttttg cgggaatgac gggattttag gtttttgttt
      121 tcgtgggaat ggatgggccc aaagccgtcc gaaccaaaaa aaaccgccgc tttcagacgg
      181 catcaaaaaa ccgcaggcac gatgcctgcg gtttgccgtt tgcgcttcag gggagcaggg
      241 ggattgcgcc caagccgtgt gtttctttca gtccgaacat gatgttcata ttttgcaccg
      301 cctgacccgc cgcgcctttg acgaggttgt cgatgacgga aaggacgacc cacacatcgg
      361 attgcgccgc ctgttggatg ctgatgcggc agaggtttgc gccgcgcacg ctgcgggttt
      421 ctggtgtcga accggcgggc aggatgtcca cgaacaggct gtcgcggtaa tagtcgcgca
      481 ggatggtttc ggggcagatg ccgtctgaaa ggtggaggta aacggtggcg tgcataccgc
      541 gtatcatcgg cgcgaggtgc ggcgtgaaca cgaatccttc ggcgatgccg tcctgaagcc
      601 cggcgatggt ctgcctgatt tcgggcaggt ggcggtgtcc ggctatgccg taggctttga
      661 agttgtcgcc gacttcgcac aacagcgaac cgacattgcc tttcctgccc gcgccggaca
      721 cgccggattt gcagtcggca atcagcggca taccgggctt cagacggcat tgccgcaaca
      781 gcggcacgag cggcagggat acgcaggtcg ggtagcagcc ggggttggcg acgaggcgcg
      841 cctgtgcgac ggcttcgcgg ttgagttcgc tcaatccgta cacggcttgg gaaacgaggc
      901 cgggggcggc gtggggcatg ccgtaccagt gttcccaggt cggaatgtcc catatgcgga
      961 agtcggcgga aaggtcgatg acgcgcacgc cctgttcaat caggcgcggc gcgtctttca
     1021 tggcgatgcc gttgggcgtg gcgaagaaga cgatgtcgca ttgttccaaa cctgcctcgt
     1081 cgggcgtttg gaaggcgagg ccgtacacgc cgcgcaaact cggaaagtaa tcggcaactg
     1141 cggttcccgc ttcgctgcgg ctggttacgg cggcgacttc gacatcggga tgggcggcaa
     1201 gcaggcgcag cagttccacg ccggtgtagc ccgtcgcgcc gacaatgccg actttgattt
     1261 ttttactcat ggtttttcct ttgtgtggtt ggcgggtatg ccgtccgaac gctgtccgac
     1321 ggggttttga ttggcggcgg aaggacggta cacaaagcgt ttgaaaagcg tgtctaattt
     1381 aatggcatcc gcctctgttt ttaaaaccca gccctgccgt ccggaataaa cataaccgta
     1441 ggcgcgaaag ccggcaggat atttcggtgc ctgccccaaa cccaaaccgt atgcggcgaa
     1501 agcggtggtc gtggagaagg aaagcaggag gaggacgagg gttttcataa ggttccaaac
     1561 cgaacggggc gatgaagccc gattatagca aaaggtcgtc tgaaaaccgt tttcagacga
     1621 ccttttattt aattacaata aaaatgttaa caacaaaaaa caaaccgctt ttttccgttt
     1681 ggagacaaat ttttaactaa aattgtcaac aattccttga cgcacacaca aaaaaaactt
     1741 taatatttgt aaatatatgt aacattataa attttaaata ttttctgagt aaggggaagt
     1801 aatgagcatc catctcgact tcggcattag tcctaaaacg ttccgacaga cttaatctgt
     1861 atcaaaagcc caagctcttt aaaggagcgg ttcggaatct cgaagccgca tcttggaaag
     1921 atatcaacga gatataccaa cgagcagacc caaccgcacc gctgttttat ctgcgtaaaa
     1981 aaggtgcaat tgttcctaaa gaagaatacg tcgaaagttt cgacgctttg ggcaaaactc
     2041 gctaccgttt tattaaatcc gttatctacg aacatatgaa gaatggtgcg tcgttagtct
     2101 ataaccatat taacaacgag ccgttttcag atcatatttc ccgtcaagtc gcccgccttg
     2161 ccggcgcaca tactattgtt agtgggtatc ttgcttttgg cagcgacgaa tcttataaaa
     2221 accattggga cacccgcgat gtgtatgcca tccagctttt cggcaagaaa cgttggcaac
     2281 ttactgcccc tgatttccct atgccattgt atatgcaaca gactaaagat actgatattt
     2341 ccattcctga acatatcgat atggatatta tccttgaagc aggtgatgtc ctctacatcc
     2401 cgcgcggttg gtggcacaga cctatcccgc tcggctgtga aaccttccac ctcgctgtcg
     2461 gtacctttcc gcccaacggc tataattacc tcgagtggct aatgaagaaa ttccccacca
     2521 tagaaagtct gcgccacagt ttctcagact gggagcaaga taggacgcgt atcaacgata
     2581 ctgccgcaca aattgctgcc atgattgccg accccgtcaa ttatgaagcc ttcagtgaag
     2641 actttctcgg caaagaacgt accgataccg cttttcatct cgaacagttc gcgaatccca
     2701 acgctactcc gctttcagac gacgtcaggt tgagattaaa tgccaataat ttggatacgt
     2761 tggaaaaggg atatttgatt gggaatggga tgaagataag cgtagatgag ttggggaaaa
     2821 aagttttaga acacatcggt aagaatgaac cgttattgtt gaaaaatcta ctggttaact
     2881 tcaatcaggg aaaatatgaa gaagttagga agttgattta tcagttgata gagttagatt
     2941 ttctggaaat tttgtgaggg attctatgaa aaactggaag caatttacat ttttcgtaat
     3001 attagtaata gcttgttatc aagtgctata ttttttatca gatatgtttc ttctcgacta
     3061 cattaacaaa tatagttgga atttgaattt tattcagggt actttgaatt ttttttcaat
     3121 atatctgcca tatgtttttg ttagccgaat ttttaggaat accaaccaag aaaaggagta
     3181 taaaaatgat tgaacttcaa cttcatgaat tgaagctggt ttcaggggga ggtcctgtaa
     3241 cagacaatat agctggaaat gtagctaatg ctgccacaac caaaggaggt cccacatggg
     3301 gggatttggt tgcaatacct gctgccgcag cggcagttta ttatctgccg aaaaatgctt
     3361 acggtgctgc tggtgcaaat ggtgtataca atgtgactcg tgattgggta aatgatgccg
     3421 ttaatgcacc tccttataac ggaagaccaa tctttgagat tgaacatgga ttaactgctc
     3481 ccgcaacaaa agcagataaa tcaggaaacg gctacactga cggtacagat tactgctgat
     3541 atttctcatt gttcagacga tctctagggg tcgtctgaaa ctttcttaac ttcaatttta
     3601 tgaatagacc caagcaaccc ttcttccgtc ccgaagtcgc cgttgcccgc caaaccagcc
     3661 tgacgggtaa agtgattctg acacgaccgt tgtcattttc cctatggacg acatttgcat
     3721 cgatatctgc gttattgatt atcctgtttt tgatatttgg taactatacg cgaaagacaa
     3781 cagcggaggg acaaatttta cctgcatcag gcgtaatcag ggtgtatgca ccggatacgg
     3841 ggacaattac agcgaaattc gtggaagatg gagaaaaggt taaggctggc gacaagctat
     3901 ttgcgctttc gacctcacgt ttcggcgcag gaggtagcgt gcagcagcag ttgaaaacgg
     3961 aggcagtttt gaagaaaacg ttggcagaac aggaactggg tcgtctgaag ctgatacacg
     4021 ggaatgaaac gcgcagcctt aaagcaactg tcgaacgttt ggaaaaccag aaactccata
     4081 tttcgcaaca gatagacggt cagaaaaggc gcattagact tgcggaagat cagtggcaaa
     4141 aacggcactg ggcagacagg aattgtcggg attgggcatg gtatcctccg atttggcgaa
     4201 gagcaacgaa cctgtttatc tcgtgaaaat aaaacccgac aaaccaacca tcactgcata
     4261 cggtgaggaa aaaccgctgc aaatcggcat gacgttggaa gcagacatcc tgcacgagaa
     4321 acggcggctg tacgaatggg tattggagct gatttatagt atgtcgggca aactgtaaga
     4381 cttgaggctg aaagataaag aagttgtctg aaaccttttc agacgaccag aagacaataa
     4441 aaaacacaaa cttcaaaact gcctgaaacc atcccctttt acaggcactc cgtttgtaaa
     4501 agcaaggata gggcatctgc ccttttgatt gtttgattaa cgatacaagg agtttcaaaa
     4561 tgagagtttt atagtggatt aacaaaaacc agtacagcgt tgcctcgcct tgccgtacca
     4621 tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatatgtgtt
     4681 catgaaatga cttgggtcgg aggctcaggt aatgcgcaac aaagctcata ttattgcgaa
     4741 atttgcgaat ctgcagggct taacgatacg ggaaatcctg ataaatcttt aggattgcca
     4801 aacaatacgt tcagtaatcc gcctggttgg ggagctacaa tcggagcttt agcaggtagc
     4861 cgcataggga tgcctgaatt tggtacgttt gcgagccatg ccattgaaaa tttcgactgg
     4921 tcatggtatc gacgttatag ggaaattgcc gaaacgattg aacgagaata ttcaggcggt
     4981 ttgccttaat agttgaggag gtcatgatgt ttgccaaaca ttatcaattc atcgcactcg
     5041 gcatcatgct gcttctttat atgttgattc tctatacgac cgatttttcc aatctgacgt
     5101 attggatgct gttttttatc tgttttatta caggaaaaat attagctcgt ttgttagaga
     5161 aaagctttaa ataaaatagc agctagtcgc aaaaggtcgt ctgaaacctt ttcaggcggc
     5221 ctttctaaaa tacatccaac ttcctaatcc ctatttttca aaaaggaaat ctatgcccca
     5281 tctgcaaaac ctgtctttgg gcttaaagaa aaagctgcct gttatcctgc aaacagaaat
     5341 atcagaatgc ggcttggcat gtctggcggc tgtggcggga tttcatggtt tccatacgaa
     5401 tttacgcgca ctgcgttcaa aatactgtcc gagacctttg caaaattccc caaaatcccc
     5461 taaatgtctt ggtgggaatt ttggggaatt ttgcaaaggt ctcattctat aactgtaaat
     5521 acttttaaat ttatgacaaa atagtaaata ttgctaaaat aatattgatg tcatgaaatt
     5581 ttttcctgct ccatgtctgt tggttatcct ggctgtcata ccccttaaaa ccttagctgc
     5641 cgatgaaaac gatgcagaac ttatccgttc catgcagcgt cagcagcaca tagatgctga
     5701 attgttaact gatgcaaatg tccgtttcga gcaaccattg gagaagaaca attatgtcct
     5761 gagtgaagat gaaacaccgt gtactcgggt aaattacatt agtttagatg ataagacggc
     5821 gcgcaaattt tcttttcttc cttctgtgct catgaaagaa acagctttta aaactgggat
     5881 gtgtttaggt tccaataatt tgagcaggct acaaaaagct gcgcaacaga tactgatcgt
     5941 gcgtggctac ctcacttccc aagctattat ccaaccacag aatatggatt cgggaattct
     6001 gaaattacgg gtatcagcag gcgaaatagg ggatatccgc tatgaagaaa aacgggatgg
     6061 gaagtctgcc gaaggcagta ttagtacatt caataacaaa tttcccttat ataggaacaa
     6121 aattctcaat cttcgcgatg tagagcaggg cttggaaaac ctgcgtcgtt tgtcgagtgt
     6181 taaaacagat attcagatta taccgtccga agaagaaggc aaaagcgatt tacagatcaa
     6241 atggcagcag aataaaccca tacggttcag tatcggtata gatgatgcgg gcggcaaaac
     6301 gaccggcaaa tatcaaggaa atgtcgcttt atcgttcgat aaccctttgg gcttaagcga
     6361 tttgttttat gtttcatatg gacgcggttt ggcgcacaaa acggacttga ctgatgccac
     6421 cggtacggaa actgcaagcg gatccagaag ttacagcgtg cattattcgg tgcccgtaaa
     6481 aaaatggctg ttttctttta atcacaatgg acatcgttac cacgaagcaa ccgaaggcta
     6541 ttccgtcaat tacgattaca acggcaaaca atatcagagc agcctggccg ccgagcgcat
     6601 gctttggcgt aacagacttc ataaaacttc agtcggaatg aaattatgga cacgccaaac
     6661 ctataaatac atcgacgatg ccgaaatcga agtgcaacgc cgccgctctg caggctggga
     6721 agccgaattg cgccaccgtg cttacctcaa ccgttggcag cttgacggca agttgtctta
     6781 caaacgcggg accggcatgc gccaaagtat gcctgcaccg gaagaaaacg gcggcgatat
     6841 tcttccaggt acatctcgta tgaaaatcat tactgccggt ttggacgcag ccgccccatt
     6901 tattttaggc aaacagcagt ttttctacgc aaccgccatt caagctcaat ggaacaaaac
     6961 gccgttggtt gcccaagata aattgtcaat cggcagccgc tacaccgttc gcggatttga
     7021 tggggagcag agtcttttcg gagagcgagg tttctactgg cagaatactt taacttggta
     7081 ttttcatccg aaccatcagt tctatctcgg tgcggactat ggccgcgtat ctggcgaaag
     7141 tgcacaatat gtatcgggca cgcagctgat gggtgcagtg gtcggcttca gaggagggca
     7201 taaagtaggc ggtatgtttg cttatgatct gtttgccggc aagccgcttc ataaacccaa
     7261 aggctttcag acgaccaaca ccgtttacgg cttcaacttg aattacagtt tctaacctct
     7321 gaatttttta ctgatattta gacggtcttt ccttatcctc agaccgtcaa actttaccta
     7381 cgtacttggc gcgcagtacg ttcatcttca aaatggaata gacatgaata aaggtttaca
     7441 tcgcattatc tttagtaaaa agcacagcac catggttgca gtagccgaaa ctgccaacag
     7501 ccagggcaaa ggtaaacagg caggcagttc ggtttctgtt tcactgaaaa cttcaggcga
     7561 cctttgcggc aaactcaaaa ccacccttaa aaccttggtc tgctctttgg tttccctgag
     7621 tatggtattg cctgcccatg cccaaattac caccgacaaa tcagcaccta aaaaccagca
     7681 ggtcgttatc cttaaaacca acactggtgc ccccttggtg aatatccaaa ctccgaatgg
     7741 acgcggattg agccacaacc gctatacgca gtttgatgtc gacgctaaag gtgctgtact
     7801 gaacaacgat cgcaacaaca acccgtttct ggccaaaggc agtgcgcagt tgattttgaa
     7861 cgaggtgcgc ggtgcggcca gcaaactcaa tggcatcgtt accgtaggtg gtcaaaaggc
     7921 cgatgtgatt atcgccaacc ccaacggcat taccgtgaac ggcggcggct ttaaaaacgt
     7981 cggccgcggt atcttaacta ccggtgcgcc ccaaatcggc aaagacggtg cactgacagg
     8041 atttgatgtg cgtcaaggca cattgaccgt aggagcagca ggttggaatg ataaaggcgg
     8101 agccgactac accggggtac ttgctcgtgc agttgctttg caggggaaat tacagggtaa
     8161 aaacctggcg gtttctaccg gtcctcagaa agtagattac gccagcggcg aaatcagtgc
     8221 aggtacggca gcgggtacga aaccgactat tgcccttgat actaccgcac tgggcggtat
     8281 gtacgcagac agcatcacac tgattgccaa tgaaaaaggc gtaggcgtca aaaatgccgg
     8341 cacactcgaa gcggccaagc aattgattgt gacttcgtca ggccgcattg aaaacagcgg
     8401 ccgcatcgcc accactgccg acggcaccga agcttcaccg acttatctct ccatcgaaac
     8461 caccgaaaaa ggagcggcag gcacatttat ctccaatggt ggtcggatcg agagcaaagg
     8521 cttattggtt attgagacgg gagaagatat cagcttgcgt aacggagccg tggtgcagaa
     8581 taacggcagt cgcccagcta ccacggtatt aaatgctggt cataatttgg tgattgagag
     8641 taaaactaat gtgaacaatg ccaaaggctc ggctaatctg tcggccggcg gtcgtactac
     8701 gatcaatgat gctactattc aagcgggcag ttccgtgtac agctccacca aaggcgatac
     8761 tgaattgggt gaaaataccc gtattattgc tgaaaacgta accgtattgt ccaacggcag
     8821 tattggcagt gctgctgtaa ttgaggctaa agacactgca cacattgaat cgggcaaacc
     8881 gctttcttta gaaacctcga ccgttgcctc caacatccgt ttgaacaacg gtaacattaa
     8941 aggcggaaag caggtggtac tgatggctga tggcgacatt caggcaaaag cctccaatct
     9001 gaatgcatcc ggcaatctgt atattcatgc aggtaaggat ctggatttga atgccgataa
     9061 agacttatca acacaaagta tcagcctgag ggcagacaac accgccctca tcagtagcaa
     9121 cggtaacacc ttgactgcag aaaagaatct ggatattcag gcaggtagct tgagtgtgcg
     9181 tcaaagtaat ctgcaatcca gtggcggtaa tgtgcagatg agcgcgacta aaggcaacat
     9241 cagcttgaat cagagttgga taaatgccag tcagaatatt gatacggcag cacttcaagg
     9301 caatattatt tcagacggtc tgactgctgt tgccgaagtt ggacgcgtat ctcttcttgc
     9361 caacggcaat gttgatttca acggtctaaa taccttgatt gcggaaggag acattaatgc
     9421 tggttcagtc ggtaaaggcc gtctgaaaat ggacaatacc gatatttatg cttctgcagg
     9481 cgatgtgaaa ctggttgccg gaggtcaatt agatttaggc aacggcaccg ttaacggcgg
     9541 tcatatcagc ttagacagca ataaaggcag catggtggtg caggatgtac acctgaatgc
     9601 ccgcgcatca ctgaaagtgg atgcggatca aactttaacc attaataaca gcaagctcaa
     9661 ttccggtcac aatacccaga ttaatacaaa tcatggtcat atgacgctta atcagcttga
     9721 tgctcattca cgtcgtcata tgagtatcag tgcacagggt aaaggcaaag gaaaagacag
     9781 cggtcaaatt ttacaaaacg accagcaaaa cagtaaaagt actttggcgg cagatggtgt
     9841 attgtcattg aacagcagcg cattacaggt tttggacaat actaccctgc gcggtggcgc
     9901 gataaacatc aaagctggcg gaggcatcat caaacggggc tatatcgatt gggaaaccca
     9961 agatacggct actatgcgtt cagcggaact gaaaccactg tctggtatga tgtcgataga
    10021 atcgggcggt gataaccctt taaccgttga acccggtaac cgtatcgttt ctgcaggtga
    10081 tttggccgtg aaccacaacg gcacattcca aatcagtgcc agagcaggaa ataacggcaa
    10141 tccgagtgcg caaacagcca gcgtttcagc taaaggcaat atcgggattg tggcgggaga
    10201 ggtggatatt gatgcagcaa atattgccgc agggaaagat ttggctttag tggcaactaa
    10261 agggaatatt tcactaaaca gtattaggaa tacgtttagt aactatcaat tgaaaacaga
    10321 taaacacaat atcacacagc aacttactga tgtagagcag gagcttagta agttaacgag
    10381 cgatcctaaa tatcgtaaag cacaggatct tccacaaatg ttgaggcgta aatataagag
    10441 gcgtgacaaa gtattcggtg atagtgaggc cagattacgt ggtttacgag cagagataaa
    10501 tgctgctgat gaggcttggg cagaactcca atccccagta aaagcgttac ttgaacgtaa
    10561 acaattgtta caacaagccc ttttaacagt ttcccaaccg ggtagtggtc acgaaaacca
    10621 aggtagcacc cttagcggtc aaaatatcaa actgctcgct gcaggtggta tccgtatcca
    10681 gggctccaaa gttgctgcca cccaacaggc aaatatccaa gctgcaggat ttttacctgc
    10741 accagccgca gaggaactgc aagaaggtcg acttcaatca gccattgaca tcagcggtgt
    10801 atttgatact tttgaatatg gtcagcaagg cagcgataaa tacggctatg ccattttcag
    10861 caggccatct gaaatttcag gaaaaacagg tgttaccctc tctgcaccaa atgccaatga
    10921 gaacagccgc atcagtctga gtgcggcaaa catcgaagct gaaaacggca aaatcaaact
    10981 gcaatcctac ggcgaccaat cctattacgc cgggcagggc gaactctata ccttagaacg
    11041 ccgcagctac aaaaccggca aatggtacaa ccgcaaacac attaccgaag tcaaagaaca
    11101 caaaaacgcc aagcccgacg cagtaaacct cagcgcatcc caaggcatcg acatcaaatc
    11161 tggtggcaac atcgacgcct acgccaccgc attcgatgcc cccaaaggca gcattaacat
    11221 cgaagccggg cggaaattga cactctatgc cgtagaagag ctcaactacg acaaacttga
    11281 cagccaaaaa aggcgcagat ttctcggcat cagctacagc aaagcacacg acaccaccac
    11341 ccaagtcatg aaaaccgcgc tgccctcaag ggtagttgca gaatcagcca acctccaatc
    11401 gggctgggat accaaactgc aaggcacaca gtttgaaacc acactgggtg gcgcaaccat
    11461 acgcgcaggc gtaggcgagc aggcacgggc agatgccaag attatcctcg aagggatcaa
    11521 aagcagcatc cacacagaaa ccgtgagcag cagcaaatct actctatggc aaaaacaggc
    11581 aggacggggc agtaacatcg aaaccttgca a
//
LOCUS       DAWFAD010000054        11533 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWFAD010000054 DAWFAD010000000
VERSION     DAWFAD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11533
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.054"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            121..543
                     /locus_tag="WGO49_001073"
     CDS             121..543
                     /locus_tag="WGO49_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306189.1"
                     /translation="MKKTFLSAVLLLSAATQTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGWGRGRWATMKFQNGEFMYTVDAGFDSV
                     THAVGSGVLVERRGKEIARFDCTSKTAQANFDDDDFAW"
     gene            582..1322
                     /locus_tag="WGO49_001074"
     CDS             582..1322
                     /locus_tag="WGO49_001074"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ0306190.1"
                     /translation="MLIVRPSGRAKDDVETCRRAEWQAEVLSPIEIETDKASLKRLPE
                     MYARADAVFWVSPTAVETAVPYLNLSDGIKMHISVGQGSRRALERCLGRTVIAPDDGN
                     DSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFRRHKPLNF
                     QNFQTENIAAAYITSAELVQSLFAQVPPQFSRFFKSLLYFTHHPRIAEALKREGVCSV
                     ETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            1337..2671
                     /locus_tag="WGO49_001075"
     CDS             1337..2671
                     /locus_tag="WGO49_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0306191.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFDQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLILDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            2668..3885
                     /locus_tag="WGO49_001076"
     CDS             2668..3885
                     /locus_tag="WGO49_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ0306192.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            3960..5024
                     /locus_tag="WGO49_001077"
     CDS             3960..5024
                     /locus_tag="WGO49_001077"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ0306193.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     MQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGNQVALQGNFDPSALFGTPESIRAEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            5202..6581
                     /gene="radA"
                     /locus_tag="WGO49_001078"
     CDS             5202..6581
                     /gene="radA"
                     /locus_tag="WGO49_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ0306194.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSSVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQTA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(6856..7335)
                     /locus_tag="WGO49_001079"
     CDS             complement(6856..7335)
                     /locus_tag="WGO49_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ0306195.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAAHRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(7467..7826)
                     /locus_tag="WGO49_001080"
     CDS             complement(7467..7826)
                     /locus_tag="WGO49_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ0306196.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPLAAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAKAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(7865..11479)
                     /gene="recB"
                     /locus_tag="WGO49_001081"
     CDS             complement(7865..11479)
                     /gene="recB"
                     /locus_tag="WGO49_001081"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ0306197.1"
                     /translation="MSAPIQAFDPLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLVPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQTDLPQARDAALASWQNICGNLAELKETFWRIHPLLNGNSYRKNSFAELF
                     DALEQKALSPDLPFLEAKLHERLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFRRD
                     LDALEEAEEATIIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAECRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSSDTADNPLAYLIEGLPQ
                     DSRETVRQTYDWEKDGIAMLKRNWRRLTDNAPAGTDFAFTEDAPPPAAYRGNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYCLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPDGNICVIDYKSNHLGADASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         2521 a         3049 c         3417 g         2546 t
ORIGIN      
        1 tgccgcccta tgtgttgccc taatttggaa ggggttacac ccttttcaaa taaaatctga
       61 tgctgctgcc acgaaggacg gatgtccaag tggcggggtt ttaaccatta aggaaatacg
      121 atgaaaaaaa cgttcctttc tgccgtattg cttctgtcag ctgcgaccca aaccgtgtgg
      181 gcagatacgg tgttttcctg taaaacggac aacaacaaat atatagaagt tcaaaaaatt
      241 aaccgcaatc tttacgaata ttcgttcggc agtgcggcaa aaaaagaaat tgccatacgc
      301 aacagcaaag ctgatctgtt ggggcgttcc gacaggtggc aaggctgggg cagaggccgt
      361 tgggcgacga tgaaattcca aaacggcgaa tttatgtaca ccgtagatgc aggcttcgat
      421 tccgtgaccc atgcagtagg cagcggtgtc cttgtggagc gtaggggcaa ggaaatcgca
      481 cggttcgact gtacgtcgaa aaccgcgcag gcgaatttcg acgatgacga ttttgcctgg
      541 taatcggggc ggataaggcg atagaaacag tgaaacccat catgctgatt gtccgaccgt
      601 cgggcagggc gaaggacgat gtggaaacct gccgtcgtgc cgaatggcag gcggaagtgt
      661 tgagtccgat tgaaatcgaa accgacaaag caagcctgaa acggctgccg gaaatgtatg
      721 cccgtgcgga tgccgtgttt tgggtcagtc cgaccgccgt tgaaaccgcc gtcccgtacc
      781 ttaacctttc agacggcata aagatgcaca tttccgtagg gcagggcagc cgccgtgcat
      841 tggaacgctg tttgggcaga acggtcatcg cgcctgatga cggcaacgac agcgaggcgg
      901 ttttgcgcct gccggtttgg aacagtctgc ccgaaggtgc gcgcgtattg tttgtgcgcg
      961 gacacggcgg gcgggatttt ctgatgaatg ccttgcagga gaaaggtttt cggacggagg
     1021 tggcagaagt ctatttcaga cggcataaac ctttgaactt tcaaaatttc caaaccgaaa
     1081 atattgccgc cgcctatatt acgtccgcag aactcgtgca gtcgctgttt gcacaggttc
     1141 cgccgcaatt ttcccgattc ttcaaatcct tgctatactt tacccatcat ccgcgcattg
     1201 cggaggcatt gaagcgcgaa ggcgtgtgtt cggtcgaaac cgtccctacg ctggaagccg
     1261 cgctttccca ttcttccatt tccgtttcag acggcatggt ctttcccgga acctcaaatt
     1321 aataaggagc aaaacggtgg gcgaacctga aaacaaatca tccgaacccg tacgcgagat
     1381 acaggcatca aaagaaatgc cgtctgaaac ctcttcccca cgcaaagaaa acgaaacaga
     1441 agtacacatt cctgccgctc cttttatcgt caaacagtcc ggcagcaacg ctttggcagt
     1501 ctgcgccctg gtattggcgg cattgggttt gggtacaagt ggttttttgt ttgtccaggg
     1561 acagaatgtc ttgaaaaacc aagagctggc attcaaccaa aaaatcgaca aagccgcctt
     1621 gggcgagtcg gaaaacgccg ccctgttgaa agacaacctc aaccggcaag ccgccataca
     1681 atcagagctc gaccgtttgg acggaaacgt caaagcaaac ggcgaacaaa tcttggaaat
     1741 gcaaaaatcc tatcgcgagt tgaccaaagg acgcgccgat tggctggtgg acgaaaccga
     1801 gaccatactc aatctggcgg cgcaacagct ggtgttgacc ggcaatatcc aaacggcagt
     1861 cggcgtattg gagcatatcg acagccgcct gtcccgtttc gatcaggcag agcttctgcc
     1921 gatcaaacag gcagtcagca gcgacttggc ggaactgaaa aaccgtccct atgtcgatat
     1981 ttccggcacg gcattgcgcc tcgacaggct ggaaaccgcc gtatccggac tgccgctgat
     2041 tttggacggc gtattgaagc cgggcgtaca ggtgaagaac gaagccgctt ctgcttcatg
     2101 gtggcagaac gtatgggaaa aatccctcgg cacattgaag gggctggtcg aaatccgccg
     2161 tttggaaaac aacgatgcca tgctgatttc tcccgaacag gcatattttg tgcgtgaaaa
     2221 cctgcgcctc cgccttttgg atgcgcggac tgcattaatg cagcgcaaca gcgaagtcta
     2281 tcagggcgat ttgaacaatg ccgaagccgc cgtcagacag tatttcgatg ccaagtctcc
     2341 cgccacgcag tcgtggctga aagaactggc ggaattgaag gcgttggatg tgcgcatgac
     2401 ggcggatgac ggtttgaaaa acagcctaaa tgccgtccgc gcctatcgcg acggtacgcg
     2461 catgacggcg gcggaaaatc aagaagcaga acaggcggct tccgaaccgg caaacgaaaa
     2521 cacagcttcc gaaccggctg ccgcatcgga tgtgaagacc atagaagcac cgtccctgcc
     2581 ttcggaacgc aaaccggaac agcctgcaaa aaaacagccc gtaccggaaa aggcagggcg
     2641 ttcgccgtcc gctaaaggag aacgcgcatg aaaacggtag tctggattgt cgtcctgttt
     2701 gccgccgccg tcgggctggc attggcttcg ggcatttaca ccggcgacgt gtatatcgta
     2761 ctcggacaga ccatgctcag aatcaacctg cacgcctttg tgttaggttc gctgattgcc
     2821 gtcgtggtgt ggtatttcct gtttaaattc attatcggcg tattcaatat ccccgaaaag
     2881 atgcagcgtt tcggttcggc gcgtaaaggc cgcaaggccg cgcttgcctt gaacaaggcg
     2941 ggtttggcgt attttgaagg gcgttttgaa aaggcggaac tagaagcctc acgcgtgttg
     3001 gtcaacaaag aggccggaga caaccggact ttggcattga tgttgggcgc acatgccgcc
     3061 gggcagatgg aaaacatcga gctgcgcgac cgttatcttg cggaaatcgc caaactgccg
     3121 gaaaagcagc agctttcccg ttatcttttg ttggcggaat cggcgttgaa ccggcgcgat
     3181 tacgaagcgg cggaagccaa tcttcatgcg gcggcgaaga tgaatgccaa ccttacgcgc
     3241 ctcgtgcgtc tgcaacttcg ttacgctttc gacaggggcg acgcgttgca ggttctggca
     3301 aaaaccgaaa aactttccaa ggcgggcgcg ttgggcaaat cggaaatgga acggtatcaa
     3361 aattgggcat accgccgcca gctggcggat gctgccgatg ccgccgcttt gaaaacctgc
     3421 ctgaagcgga ttcccgacag cctcaaaaac ggggaattga gcgtatcggt tgcggaaaag
     3481 tacgaacgtt tgggactgta tgccgatgcg gtcaaatggg tcaaacagca ttatccgcac
     3541 aaccgccgcc ccgagctttt ggaagccttt gtcgaaagcg tgcgcttttt gggcgaacgc
     3601 gaacagcaga aagccatcga ttttgccgat gcttggctga aagaacagcc cgataacgcg
     3661 cttctgctga tgtatctcgg tcggctcgcc tacggccgca aactttgggg caaggcaaaa
     3721 ggctaccttg aagcgagcat tgcattaaag ccgagtattt ccgcgcgttt ggttctggca
     3781 aaggtttttg acgaaaccgg agaaccgcag aaggcggagg cgcagcgcaa cttggttttg
     3841 gcaagcgttg ccgaggaaaa ccgaccttcc gccgaaaccc attgaaacct tcagacggca
     3901 tcgttgaaat gccgtctgaa aaccatttca cttgacaaac agactgacag aaaacccgta
     3961 tgactctttt aaaaaacgat accttcctcc gcgccctgct caaacagccc gtcgaataca
     4021 cgccgatttg gatgatgcgc caggcggggc gttatctgcc cgaatacaaa gccacacgcg
     4081 cgaaagcggg cagcttcctc gatttgtgca aaaacaccga attggcgacc gaagttacca
     4141 tccaaccttt ggaacgcttc gatttggacg cggcgatttt gttttccgac atcctgaccg
     4201 tccccgacgc aatgggcttg ggactgtatt ttgccgaagg cgaaggcccg aaattcaaac
     4261 gcgccatgca acacgaggcc gacatcgcca agctgcacgt tcccgatatg gaaaaactgc
     4321 aatacgtttt cgacgcggta acttccatcc gtaaagcatt ggacggccgc gtaccgctta
     4381 tcggcttttc cggcagcccg ttcacactcg cctgttatat ggtcgaaggc ggcggcagca
     4441 aagaattccg caccatcaaa accatgatgt actcgcgccc cgatttgctg cacaaaatcc
     4501 tcgataccaa cgcccaagcc gttaccgcct acctcaacgc ccaaatcgac gcgggcgcgc
     4561 aggcggtgca aattttcgac acttggggcg gcgtgttgag cgacgcggcg tttaaagaat
     4621 tcagcctcaa atacatccgc cagatcgtcg ccggactcaa acgcgaaagc gaaggccgcc
     4681 gcgtgcctgt tatcgtattt gccaaaggcg gcgggctgtg gctggaaagt atggcccaaa
     4741 tcggcgcaga cgcattgggc ttggactgga cgtgcaacat cggcgaagca cgccgccgcg
     4801 tcggaaacca agtcgccctg caaggtaact tcgacccgtc cgccctcttc ggtacgccgg
     4861 aatccatccg cgccgaagtc gcacgcatcc tagccgacta cggacacggc agcggccatg
     4921 tcttcaacct cggacacggc atcaaccaac atgccgaccc cgaacacgcc aaaatcttag
     4981 tcgacaccgt acacgagctg tcgcggcagt atcacggcgg gtaagccggc aggaaaccgc
     5041 ccgatatgcc gtctgaagcc gagggatgga aaaataaggc aatgcggcaa tatccgccgt
     5101 gtatggatag tacatgacgg cggcggtgtt gccgcattgg cgcaatccca accgtcccta
     5161 ttttcagacg gcatttttgt tttcagatgc agggaaaacc gatggcaaaa acgcttaaaa
     5221 ccctttacca atgcaccgaa tgcggcggca cttccccgaa atggcagggc aaatgcccgc
     5281 attgcggcga gtggaacaca cttcaggaaa gcttggccgc gcccgagccg aaaaacgccc
     5341 gcttccaatc ttgggcggcg gatacctcga ccgtccaatc cctctcctct gttaccgcga
     5401 ccgaagtgcc gcgcaatccg accggtatgg gcgaactcga ccgcgtattg ggcggcggtt
     5461 tggtcgatgg tgcggtcatc ctgctcggcg gcgatcccgg catcggcaaa tccacgctgc
     5521 tgttgcaaac cattgccaaa atggcgcaaa gccgcaaagt gctgtacgtt tccggcgagg
     5581 aatctgccca acaggtcgcc ctgcgcgcgc aacgtttgga actgcccaca gaaggtgtga
     5641 acctgcttgc cgaaatccgc atggaggcga ttcagacggc cttgaaacag catcagcccg
     5701 aggtcgtcgt catcgactcc atccaaacca tgtattccga ccaaatcact tccgccccag
     5761 gctccgtgtc gcaggtgcgc gagtgtgccg cccaactgac gcgcatggcg aaacagatgg
     5821 gcatcgccat gatactggtc ggacacgtga ccaaagacgg cgcgattgcc ggcccgcgcg
     5881 tgctggaaca catggttgat accgtgctgt atttcgaggg cgaccaacat tccaactacc
     5941 gcatgatacg cgccatcaaa aaccgcttcg gcgcggcaaa cgaactgggc gtgttcgcga
     6001 tgacggaaaa cggtttgaaa ggtgtgtcca acccgtccgc catcttcctc gccagctacc
     6061 gcgacgatac gcccggctcg tgcgttttgg ttacacagga aggcagccgc ccgcttttgg
     6121 tcgaaattca ggcattggtc gatgacgcgc acggcttcac gcccaaacgc ctcaccgtcg
     6181 gactggaaca aaaccgtctt gcgatgctgc ttgccgtgtt aaaccgccac ggcggcatcg
     6241 cctgtttcga tcaggatgtg ttcctcaacg ccgtcggcgg cgtgaaaatc ggcgaaccgg
     6301 cggcggattt ggcggtcatc ctcgcgatgc tttccagctt ccgcaaccgc cctatgcctg
     6361 aaaaaaccgt ggttttcggc gaaatcggct taagcggcga agtccgcccc gtcgcacgcg
     6421 ggcaagagcg gctcaaagaa gcggaaaaac tcggcttcaa acgcgccatc gttcccaaag
     6481 ccaatatgcc gcgcaacgcc aaagagtttc cgaacctgaa aatctacggc gtttcgagtt
     6541 tgcaggaagc catcgatatt tgccgtgaca gcagggaata aacggaaatg ccgtctgaaa
     6601 tcaggtttca gacggcattt ggtttgtggc ggattgaaac aagaaggcat accggcgaca
     6661 gataagattt gcggcaaagt tgcctgtgat atggcaaaac gcacacgccc gtcatccccg
     6721 caagggcggg aatccggaat cgtccgtttc ggcaatgatt gaaaatcacg gtaacccaac
     6781 cgcttggatt cccgacttcg tgggaatgag gggcgtgtgt gtttgatttc catccgccat
     6841 atgtcggcga cgggcttatt cgcctacggt tttttgtatc agtttttcgg cgtttgccaa
     6901 agtgtttgcc acttcgtcaa aaccgatgcg gctgccggcg atgagggcgc gcgtatcggt
     6961 ataggcggcg cgtactttgc cgtctgtttc ggtaacgagg acgcgcaggg gcagttgcag
     7021 ggcgaaggcg gggtctttga ccatcagcgg cgtgccggct ttgggcgtgc cgaagacgat
     7081 gacttttgcc ggctgcatcg ttaaaccgtt tcggtgggcg gcttcctgat ggtcgatgac
     7141 ggcaaaaatg tccatccctt tgctttttat ggcggtttca aggcggctga cggtttcgtc
     7201 aaaactgtat tttgaggtga gggtatgcgt ggtcatagcg gtttcgtttt gggtttgcgg
     7261 ttcgctggca ggatgtgccg aagcggttga aatgcagagt gcggatgcgg caatcagggg
     7321 gagtatgtgt ttcatcgtat ttcctttttc cttttcggtt tgtttatcag gcttcggacg
     7381 gggcggcacg cgccgcgccc gatgttgcgc cgcgtgctgg ggtgtcgtat gccgtctgaa
     7441 agcctgtcct ttcagacggc attgcgtcat ttcatccctt ttttgagcag gtcttcataa
     7501 ccgccgtgat tggcaacatt tgtataacct gcttttttca gctcctgaag ggcggctttg
     7561 gcacgccgtc cgctgcggca gtagaggttg accggcgtgt ctttgtcggg cgcggcttcg
     7621 tgtatgcggc ggacgatttg gtcgacgggg atgttgaccg cgttgtgcaa atgaccttcg
     7681 ctaaattcct gttcggaacg gacatcgatc caaacggccg gatgttgcgc ggtttgggcg
     7741 gcggctaggg gtttttgcgg ggctgcctgc gcggcaaagg cggctgaggc aatgagtgcg
     7801 gcggtaatca ggtgtttgat attcataggg ttctcctgcg gttgtcgtct taagggacgg
     7861 gaagttattt tatctgttcc aaagcggcgg catctatgtc ccaacgccaa acgccgccgc
     7921 ctttgccatc caatccgcgc aaaaacaggt agcggacgga aacggcggcg ggcggttgtc
     7981 cgcgcagttt gaagtagcgt gcggcggcaa cggcgtaaat cagcgcctga aggtaatagt
     8041 ggtggtgcgc gacggcttcg tccatcgcct gttgcgtgta ggcggatgcg tccgcgccga
     8101 gatggtttga tttgtagtcg atgacgcaga tattgccgtc ggggtcttgg cagaccatat
     8161 cgataaagcc gtttaaaaag ccgttgacgg tgtggaagtc gagcgtttcg gcggcggcac
     8221 ggcagacttc gggcaatcgg atatcgtcgc gggcaaacca gtcgcgcagc cgtttgaggc
     8281 cgaagtcttc ggtgtggagg gtaaagccca tttcgggaca gcggcactcg ggcgggatgt
     8341 cggacaggca gtatgccccc gtcagcggcg ttttgcggca ggcttccgcc atttcggcaa
     8401 cggcaggcag ccatatttct tcaaatccgt attttttcag cttgtcggca atgagagttt
     8461 cctgtccggc ggctgcttgt ccgaatttga aatcttcaag aatttcgtgc aggcacagcc
     8521 ccgcctgcgt gcctttcgga aaatcgtgta tcgatatgcc gtctgaagcc gtcggcgttt
     8581 cagacggcat cgccggcacc gaggtttcgg cggcatccaa ggacgggcag gcatcttctt
     8641 cgccgccgtc gggcgtttgg gtatggcggc ttaaggcggt aaagctggtg tggcggacaa
     8701 atcggaatct gcgttcggga atgctgtttg cggcaaattc ggcggtttga ccggcgttgc
     8761 cgcgataggc ggcagggggc ggcgcatctt ctgtgaaggc gaaatccgtg ccggccgggg
     8821 cattgtccgt caaacgccgc cagttgcgtt tgagcatcgc gatgccgtct ttttcccagt
     8881 cataggtttg gcggacggtt tcgcggctgt cttgcggcaa gccttcaatc aggtaggcga
     8941 gggggttgtc ggcggtatcg gaagaatagg cggcgtagat gttgagctgt tcctcggcac
     9001 gcgtcagcgc gacataaagc aggcgcaggc gttccgccat ttcctcatcg gcgtattgct
     9061 tctgttcgtc ttccgacagt tgcgcctttg ccaacagttc ggttcgattt gcgctttggt
     9121 ggaggatttg ccagtcggac ggcccggtat cttgcgcgtc ccacgcaaac gggcagtaca
     9181 ccagcggata ctgcaaacct ttcgaggcgt gcatggtaac gattttgacc aaatcttcgt
     9241 cgctttccag acggatggcg cggttgtcgc cgctgttgtt gccggccagg ctgatttggt
     9301 cgcgcaacca tttgtgcagc gcggcggggt tgcggttttg cgcgtcttcg gcggcaagca
     9361 gttcgagcag ttggaaataa ttggtcaggc tgcgcccgtt gttccggctt aagaggcgcg
     9421 tttcgatgcc gtgtgtttgg gaaaattgct gcatggcggc gaaaatgccg tattgattcc
     9481 agttgtcgag cgcggttcgg gcagattccg cccaatgcaa aatctcgctt tcgttttggt
     9541 tgaagtcgtg caattgctgc gcgtcataac cgaatatgcc gcttgtcagg acaaagcgca
     9601 gcgttccggc gcggcgcggt tcgagccaga agccgatgag tgcggaaagg gcggcggctt
     9661 cgggcgaggc gaacacggat tcgcgcgaaa gcaggacgct ttgcacctgc cgttttttca
     9721 gggcagcgga aaccatcacc gcctcgttgt gcgtgcgtac cagcacggca atatcgcccg
     9781 actgcaacgg gcagcctttg aaattcagac ggcattcggc ggcttcgttg agcgcgtggg
     9841 cgatttcgtc ggcgcaatag tcggcggcac ggcggcgcaa aacgtctttg ttggcttttt
     9901 cattgtcgtt ttcgtgcagc caacgaacct gtacggcagg acgttcgggg gacaacctgc
     9961 tttcggcacg cgccgcaccg acttccgaat agccgatgtt ttccaaaacg aacgggcgtt
    10021 ctttgaggcg gaacagcgcg cctatgctgc cgataagcgc ggcgtggctg cggtagttgg
    10081 tggcgagcgt gtagcggtgc cgcgcgtctt ccgccgcctg aaggtaggcg taaatgtccg
    10141 ctccgcgaaa gctgtaaatc gcctgtttgg gatcgccgac gaggaacagc ggtcggtttt
    10201 gggcgatgaa gattttttgg aagatttcgt attgcagcgg gtcggtgtct tggaactcgt
    10261 cgatcagcgc ggtttcccag ttttcggcaa cggcgcgggc gagggtttcg gcgtgcggat
    10321 tgtcggtcag cgcggtgtgg acatcgagca gcaggtcgtc gaaaccgcgt tcgcggcgcg
    10381 attttttcat ctcggcaagg ctgcggttga ggtatccgat taaatccagt tgcagccgga
    10441 ttattgttgc ttcttccgct tcttcgagtg cgtccaaatc acgcctgaag tctgccagtt
    10501 tctgcaattc ggcaaatact gccgcatcgg gcgttttgcc ttttttcagt ccggcttcga
    10561 gtttgtcgga cgcaagtttc aagagccgtt cgtgcagctt tgcctccaaa aagggcaaat
    10621 ccggcgacag ggctttttgt tccaatgcgt caaacagttc ggcaaaactg tttttccgat
    10681 atgaattgcc gtttaaaagg ggatggatgc gccaaaaagt ttcttttaat tcagccagat
    10741 ttccgcaaat gttttgccag cttgccaaag cggcatcccg ggcttgcggc aaatcggttt
    10801 gcggacggcg gaaattcagg tacgggcggg acaggtaggc gcggatttgg gcaaggacgg
    10861 tttgcggcac agctttgcgt ttaaacgcca atgcggcaag caccggatca ttgctgacgc
    10921 gttcccgcca aaaatcttgc gccgggacaa gcaggcggtc gccgtcttct tcggtcagtt
    10981 cgacatcgaa cggtgcttgg cacaaaaacg cgtagtcgcg caggatgcgc tggcagaagc
    11041 cgtggatggt atagatggcg gcgttgtcga attgcccgat ggcggctttg aggcggacaa
    11101 tcagacgcgt ccggccctct ttttgcaaag cctgttttaa gagtgcgggc aggaaggtgt
    11161 cgcctttgtg gtgttcggcg cagtaggcgg cgatgccgtc tgaaagcgtg tcgtctccaa
    11221 gtttggcaat ttctttactt tctaaaacct gcaacacatc gtccaaacgt gcgcgcaggc
    11281 gtgttttcaa ctcggcggtg gcggctttgg tgaaggtaac gaccaatacg cgctcgacgt
    11341 ttttttgttc caatacgatc aggcgcgtaa acagggcggc aatgccgtag gtttttccgg
    11401 tgccggcaga ggcctcaatc aggttggtgc cggcaatggg gacggtcagc gggtcaaatg
    11461 cttggatggg tgcagacata gtgcgcgctc ggaaaacggt tggacggtat agtggattaa
    11521 caaaaatcag gat
//
LOCUS       DAWFAD010000055        10807 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWFAD010000055 DAWFAD010000000
VERSION     DAWFAD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.055"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(227..1861)
                     /gene="groL"
                     /locus_tag="WGO49_001082"
     CDS             complement(227..1861)
                     /gene="groL"
                     /locus_tag="WGO49_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ0306198.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVDELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGVVISEEVGLSLEKATLEDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAMPDMGGMGGMGGMM"
     gene            complement(1954..2244)
                     /locus_tag="WGO49_001083"
     CDS             complement(1954..2244)
                     /locus_tag="WGO49_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ0306199.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            2517..3125
                     /locus_tag="WGO49_001084"
     CDS             2517..3125
                     /locus_tag="WGO49_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0306200.1"
                     /translation="MLRFFVLEVTARSAADILGIHPNSAALFYRKIRMVINHHLALAA
                     DEVFEGSVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRVYTVVVDNAKSETLLP
                     VIKKKIMPDSIVYTDSLSSCDKLDVSGFIYYRINHSKEFADRQNHINGIENFWNQAKR
                     VLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            3338..4873
                     /locus_tag="WGO49_001085"
     CDS             3338..4873
                     /locus_tag="WGO49_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0306201.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            4870..4962
                     /locus_tag="WGO49_001086"
     CDS             4870..4962
                     /locus_tag="WGO49_001086"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ0306202.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(4998..6242)
                     /gene="lysA"
                     /locus_tag="WGO49_001087"
     CDS             complement(4998..6242)
                     /gene="lysA"
                     /locus_tag="WGO49_001087"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ0306203.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDATKTIFSGVGKNEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTASVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQDENVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGS
                     LLTRVEFVKYGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACLQAEHQNAV"
     gene            complement(6253..6423)
                     /locus_tag="WGO49_001088"
     CDS             complement(6253..6423)
                     /locus_tag="WGO49_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0306204.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDTARFGAIQTGLQL
                     QNKPQSAPQTRK"
     gene            6494..6817
                     /gene="cyaY"
                     /locus_tag="WGO49_001089"
     CDS             6494..6817
                     /gene="cyaY"
                     /locus_tag="WGO49_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ0306205.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDGRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            6848..7267
                     /locus_tag="WGO49_001090"
     CDS             6848..7267
                     /locus_tag="WGO49_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ0306206.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCVNDYIHAVYDFVEQ
                     AGYCRNGFPEAGGEWVKVENRVLDDELLDKILSREPT"
     gene            7289..7795
                     /gene="luxS"
                     /locus_tag="WGO49_001091"
     CDS             7289..7795
                     /gene="luxS"
                     /locus_tag="WGO49_001091"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ0306207.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCIPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVGNVKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNA"
     gene            7941..10733
                     /gene="polA"
                     /locus_tag="WGO49_001092"
     CDS             7941..10733
                     /gene="polA"
                     /locus_tag="WGO49_001092"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ0306208.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSGETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVMEHAS
                     EIKGKVGENLRAALPRLPLSYDLVTIKTDVDLHAELSDGIESLRRTAPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDVFGSDSIGEQAALNAEMPSEKQAEKATAPERLDYQA
                     VTTEAQFAALLDKLSRANTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLGNPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAADQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGTAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNLSAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVRNCLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
BASE COUNT         2441 a         2877 c         2902 g         2587 t
ORIGIN      
        1 ttcgcgggaa tgacggcgga aaggttgctg tttttccgat aaattcctgc cgctcttcgt
       61 ttttgggatg gcgggaaata aaacaaaagc gcgcgtatca aaaaataaca atgcaaagaa
      121 cggtgttggc aattttaagg tttgcgcctt agccgcactt attcgcaaaa aaaccgcacg
      181 gcgttgaccg tgcggcttgt tgtctgaagg cttcagacgg cattgcttac atcatgccgc
      241 ccataccacc catgccgccc atatcaggca tagccggttt gtcttcaggg atttcagcaa
      301 tcatgcagtc tgtggtcagc atcaggccgg cgatagacgc ggcgtgttgc agcgcggaac
      361 gggttacttt ggcggggtcg agtacgccca tttcgatcat gtcgccgtat tcgccgctgc
      421 cagcgttgta accgtagttg cctttgcctt ccaacacttt gttcacaacc acgctaggtt
      481 cgccgcctgc gttggcaacg atttggcgca gcggagactc aacggcgcgc aagacgattt
      541 gtacgcctgc gtcttggtcg gcattgccgg tgtgcaggtt ttccaaagcg gcacgggcgc
      601 gcaacagggc tacgccgccg cctgcgacca cgccttcttc aacggctgcg cgggtagcgt
      661 gcagcgcgtc ttccacgcgg tcttttttct ctttcatttc gacttcggta gcggcgccga
      721 ctttgatcac tgccacgccg cctgccaatt tagccacgcg ctcttgcagt ttttctttgt
      781 cgtaatcgct ggttgcggtt tcgatttgtt ggcggatttc ggcaacacgc gcttcgattt
      841 gggctgcgtc gccaaagccg tcgatgatgg tggtgttttc tttaccgatt tcgatgcgtt
      901 tggcttgacc caagtcttcc aaagtcgctt tttccagaga caggccgact tcttcggaaa
      961 tcaccacgcc gccggtcagg atagcgatgt cttgcagcat cgctttgcgg cggtcgccga
     1021 agcccggagc tttgacggca acggttttca ggatgcctcg gatgttgttc accaccaaag
     1081 tcgccaaggc ttcgccttct acgtcttcag cgataatcaa cagcggacgg ctggctttcg
     1141 ccacttgttc caaaacaggc agcaggtcgc ggatgttgct gatttttttg tcaaacagca
     1201 atacaaacgg attgtccaaa gcagcgattt gtttttcagc atcgttgatg aagtaagggg
     1261 acaggtagcc gcggtcgaac tgcatacctt caaccacgtc cagctcgttt tccaaagatt
     1321 tgccgtcttc aacggtaatc acgccttctt tgccgacttt ttccatcgct tcggcgataa
     1381 tcgcgccgac ttgttcgtca gagttggcgg aaatcgagcc gacttgggcg atttctttgg
     1441 aagtatcgca aggtttggcg atgtttttca gctcgtcaac caaagcggcg acggctttgt
     1501 cgataccgcg tttcaggtcg gtcggattca tacctgcggt aacatatttc atgccttcgg
     1561 caacgatgga ttgtgccagt acggtggcgg tagtcgtacc gtcgcccgcc acgtcgttgg
     1621 ttttggacgc aacttctttc accatttgcg cgcccatatt ttcaaacttg tctttcagtt
     1681 cgatttcttt ggcgacggtt acgccgtctt tggtgatgtg cgggccgccg aaagcgcggt
     1741 caaccactac gttgcggcct ttgggaccca gggttacgcg cacggcgttt gccaaaatgt
     1801 tcacgccgtt taccattttt tggcggactt cattgccgaa ctgtacgtct tttgctgcca
     1861 tttcaattct ccaaaaatca ttaaaactgt ctgataaaac cgtttatgcc gtctgaaggc
     1921 ggtttgccgt ttcagacggc atcgtgtcca tatttatttt tcaacgatgc cgaagatgtc
     1981 ttcttcgcgc atcaccaaca gctcttcgcc gtcggctttg acggtttggc cgctgtattt
     2041 gccgaagatg attttgtcgc cgactttgac atccagcgga cggcgcgcgc cgtctttacc
     2101 gattttgccc gcgcccacgg cgatgacttc gcccatatcg ggtttttcgg cggccgcacc
     2161 cggcaaaacg atacccgatg cggttttttc ttcagcttcc aagcgtttga cgacgacgcg
     2221 gtcgtgtaaa ggacggatgg tcatatttat gctccgataa aatggtttga aaacaatcat
     2281 ctgcccgaac ggttcggaca gattgaagtg gaaaccgggc tgccgcctgc cggccgcccg
     2341 tatgaatcgg caaaattagg gtgtgtgcgg gcaaattcaa gtgaggcgga aaaaatttat
     2401 ttccgccgtt ttttatagtg gactaaattt aaggggctgt actagattag cagatatgtt
     2461 accctcgaaa tatgaagata acgcactgca aatgaaagaa aaaagtacag aaagaactgc
     2521 tccgtttttt tgtactcgaa gttaccgccc gttctgccgc cgatattttg ggtatccatc
     2581 ccaattcggc agcactgttc taccgtaaaa tccgcatggt tatcaaccat catttggcct
     2641 tggctgccga tgaggttttt gagggctctg tcgagccgga cgaaagcgat ttcggcggac
     2701 ggcgtaaagg cagacgtggt cgcggtgcgg caggaaaagt ggttgtcttc ggcattctga
     2761 aacgcaacgg acgggtctat accgttgtgg tggataatgc caagtctgaa acgttactcc
     2821 ctgtcatcaa aaagaaaatc atgccggaca gtattgttta taccgatagt ctgagcagct
     2881 gcgacaagtt ggacgtgagc ggtttcattt attaccgcat caaccattcc aaggaatttg
     2941 cagaccgtca gaaccacatt aacggcattg agaatttttg gaatcaggca aaacgtgtct
     3001 tgcgaaaata caatggaatc gatcgcaaat ctttcccgct gttcttgaaa gaatgcgaat
     3061 ttcgatttaa cttcggcaca ccgtctcaac agcttaaaat cctgcgggat tggtgtggga
     3121 tttagggcta atctagtaca gcccctaaaa tttttcgttt tcaagccttc accgcttgcc
     3181 atcagcgtta aattttttta cgataagcac atagattgta aacaatcggc cacaagccgg
     3241 tttgtttttt cagaagacat tatccctgtc agacgctatt tctatatatt tcgcctataa
     3301 tggcttgttt ttaataaata attcaagagg tatcaacgtg tctgattcca agacgaaaga
     3361 acgcgccaca ttcggcacgc gccgcgcgtt tatgattgcc gccatcgggt ccgccgtcgg
     3421 cttgggcaat atttggcgtt tcccctatat tgcttttgaa aacggcggcg gcgcgttcat
     3481 cctgccctat ctggtcgcgc ttctgacggc gggcatcccg ctgctgctgc tcgattatgc
     3541 catcggccac cgttaccgtg gttctgcgcc cttggctttc cgccgcctcg gacgatggtt
     3601 tgagccggtc ggctggtgga acgtgatgac caatatcgtc atctgcatct attacgcggt
     3661 aattatcggt tgggcggcaa gctataccta ttattcggtc aacgccgcct ggggtgcgga
     3721 tccgcagggt tttttcttta aggacttcct gcaaatggcg ggcccggaag ccttgggttt
     3781 ggattttgtc ggcaaagtcg ccggtccttt ggcgggcgtg tgggttttta ccgccgccat
     3841 tatggcgttg ggcgtgcaaa agggcgtggc gcgcgcctcg tcgttcttta tgccgctgct
     3901 tttggtgatg tttttgatta tggtcggcat ttcactaacc ctgccgggtg cggcaaaggg
     3961 cttggacgca ttgtttacgc ccgactggtc gaaactcgcc gattccaagg tctgggtggc
     4021 ggcatacggg cagattttct tttcgctttc catctgcttc ggcattatgg ttacctattc
     4081 ttcttatttg aagaaaaaaa ccgacttggg cggaacgggg ctggtggtcg gttttgccaa
     4141 cagcagcttt gaactgctcg cgggcatcgg cgtgtttgcc gcattgggct ttatggcgca
     4201 ggcgggcggt aaggcggtca acgaggttgc ctcaggcggc atcggtttgg cgtttatcgc
     4261 ctttccgacc attatcaacc aggcaccgat gggctggctg atcggcatat tgtttttcgg
     4321 ttcgctggta ttcgccggcg ttacgtcgat gatttccatc cttgaagtga ttgtggcggc
     4381 gattcaggac aagctgaaca tcgggcgcgt caacgccacg ctgctggtct gcattccgat
     4441 gggcattgtt tccacgctgc tgttcggtac ggcgacgggg ctgccggttt tggacgtgat
     4501 ggacaaattc gtcaacacct acggcattgt tgccgccggc tttgtttatg ttgccgccat
     4561 catcatcagc ggcaggctgc cggaattacg caagcacctg aacgctttgt cctccatccg
     4621 catcggcggc ttgtggacgg tctgcgtcgt ggttaccgtc gtgatgctcg gctatatgct
     4681 gtttaaagat accagcggcc tgatggagaa aaattacgaa ggttatccgg atggtttcct
     4741 cagtattttc ggctggggga tgtcggcggc gttggtcgtg ttcgggctgc tgctgtcgtt
     4801 gctgccttgg aaacacggtc aggatttcaa cgtcaaagac gaacacgaac atgaacaagg
     4861 agaagaaaaa tgagtacttc cgccattgtg atgatgattg tctcaatcgt gataatctgg
     4921 ggagggctgc tgctttccct gttaaggctg ccgaacgagt aagcctttag agcgttaaaa
     4981 atgccgtctg aaccgcttca gacggcattt tgatgttcgg cttgcaggca ggcgagttcg
     5041 tttgccattt gctgttccaa ggtttcgcgc cggcggatga gtcggtattc gttgccgtcc
     5101 accaacacct ctgccgcacg gttgcgcgcg ttgtaattgc tcgccatact ggctccgtat
     5161 gcgcccgcgc tgcggataag cagcaaatcg ccttcttcgc aggcgatggt gcggtctttg
     5221 ccgaggaagt cgccggtttc gcaaatcgga ccgacgatgt tggcggtcag aggctcaatg
     5281 tctttggttt cgaccgcctc gatgtgatga taggcatcat aaagcgccgg gcgcatcaaa
     5341 tcgttcatcg ccgcatcgac catcacaaag tttttctctt cgccgtattt gacgaattcg
     5401 acgcgtgtca gcagcgaacc tgcgttgccg accaagctgc ggccgggctc aagaatgagt
     5461 ttcagacggc gtgtgccgat cagtttttga acggcttggg catacgcgcc caaatcaggc
     5521 acattttcgt cttggtaaac aatgccgacg ccgccgccca agtccaagtg ttccaacaca
     5581 atgccttcgg cggcaagcgc gtcaaccaaa atcaaaatgc gctcgcaggc ttcgaccagc
     5641 gggcttaagt cggtcagttg cgaaccgatg tggcagtcga tgccgatgat tttcaaattg
     5701 ggctgttgtg cggcatggcg gtaggcttcg agcgcgtcgg cgtaggcaat gccgaatttg
     5761 ttggctttca gacctgtgga gatgtaggga tgggttttcg catcgacatc gggattgacg
     5821 cgtagggaga cggacgcggt tttgcccaaa cgcgcggcaa ccttttgaat acggtcgatt
     5881 tcgggaatgc tttccatatt gaagcatttc acgcctgcat tcagcgcgaa ctcgatttcc
     5941 gcctcgtttt tgcccacgcc ggaaaaaatc gttttcgtcg cgtcgccgcc tgccgccaaa
     6001 acgcgtgcca attcgccgcc ggacacaatg tcaaaaccgc tccccaaaga agcaaagtgt
     6061 ttgatgatgc tcaggttgcc attggcttta accgcatagc agacgagcgg ggagagctcg
     6121 gcaaaggcag tttggtagtg ttcaaatgct tcggtcagcg cggattggct gtacacataa
     6181 agcggtgtgc cgaatgcttc ggcaaggcgg gggtagggga cttgttcgca aaacagggtc
     6241 atatcttcgt tttcattttc gggtttgtgg ggcggattgc ggtttgtttt gaagctgcaa
     6301 accggtttgg attgcgccga aacgcgccgt atcgccttct ttgggcaggt agaggtcgcc
     6361 tttgtaaccg caggcggaaa gcaggagggc ggttgccgcc gcaaaaaata cgccgtattt
     6421 catcggtaaa cttccttcat aagcgcgaat gtggcaagat tcggtatctt aaacaaaaac
     6481 cacacaaaaa gccatgatga ccgaaagcga gtttatccgc gcgagcgaag cattatttga
     6541 acacatcgaa gaccaaatcg acgaaaacgg ctgggatttc gactgccggt ttgccggaaa
     6601 cgtcctgacc atcgaagccg aaaacggcac gcaaatcatc gtcaaccgcc acacgcccaa
     6661 ccaagaatta tggattgccg ccaaaagcgg tggctaccat ttcgccgaac aaaacggcaa
     6721 atggctggca acgcgcgacg gacgggattt ttacgacgtt ttaaacgaag ccctgagcgc
     6781 ggcttcgggc gaagcggtag agattgagga attgtgattc aatttgatat aaaaagattg
     6841 ttacccaatg gcacaattac ctctatatcg gactgccgaa atcggaaatt ttactgtcgg
     6901 cacgcctaaa gttttagaat cattttccaa acatatccct tatggtgtcg tctttgaaga
     6961 cgacggcgac acgggctact tctatgctgc ttcgcaagac gggattttag atgccttgca
     7021 catctacaat gtcgaagatg tatccgacaa acatatcccc aatcatgtct tgattttatg
     7081 ggatgatgcc tgcaccatag ccgcattgtg tgtcaacgac tacattcatg ccgtctatga
     7141 ttttgtcgaa caggcaggat attgccgcaa cggcttccct gaagcaggcg gcgaatgggt
     7201 gaaagtcgaa aaccgcgtct tggatgatga attgctggac aaaatcctat cccgagaacc
     7261 tacataaccc tcacaaaagg atacccaaat gcccctacta gacagtttca aagtcgatca
     7321 cacccgtatg catgcccccg ccgtacgcgt ggcgaaaacc atgaccacgc ccaaaggcga
     7381 caccattacc gtgttcgacc tgcgcttttg cattcccaac aaagaaatcc tgcctgaaaa
     7441 aggcatacac acgctggagc atttgttcgc aggttttatg cgcgaccact tgaacggcaa
     7501 cggcgtggaa atcatcgaca tttccccgat gggctgccgc accggtttct acatgagcct
     7561 tatcggcacg ccttccgaac agcaggtcgc cgatgcatgg ctcgcctcga tgcaggatgt
     7621 gggcaatgtc aaagaccaaa gcaaaatccc cgagttgaac gaataccaat gcggcactta
     7681 tcaaatgcac tcgctcgccg aagcgcagca aatcgcgcaa aacgtgttgg cgcgcaaagt
     7741 ggcggtgaac aaaaacgaag agctgacgct ggatgaaggg ctgctgaacg cctaatccgc
     7801 caaaaatgcc gtctgaacaa gggtttcaga cggcatttgc cttttccgtt ataatccggg
     7861 gttgtgcggg gtcgggtttt aagccggcat cgtccttccc tatttttttc tgtcccttat
     7921 cggttttaag cgggtttttt atgtccaaca gacctacact cctcctcgtt gacggctcgt
     7981 cctacctcta ccgtgcgtat cacgcgatgg ggcaaaacct gaccgccccc gacggcgcgc
     8041 cgacgggtgc gctgtatggc gtattgaata tgttgcgccg tttgcggtcg gaatatccgc
     8101 acgattattg cgcggtggtt tttgatgcga aaggcaaaaa tttccgccat caaatgtttg
     8161 aagaatacaa ggcgacgcgc ccgccgatgc ccgacgattt gcgcccgcag gcggaagcac
     8221 tgccggattt agtgcgcctg acaggctggc cggtattggt gattgggcag gtggaggcgg
     8281 acgatgtgat cggcacgctg gcgaaacagg gggcggaaca tggtttgcga gtcattgttt
     8341 cgaccggcga taaggacatg gcgcagttgg tggatgagcg cgttacgctg gtgaacacga
     8401 tgagcggaga aacgctggat attgaaggcg tgaaggcaaa attcggcgtg cgccccgacc
     8461 aaatccgcga ttatctcgcg ctgatgggcg acaaggtgga caacgtgccg ggcgtggaaa
     8521 aatgtggccc gaaaacggca gtgaagtggc tggaagccta cggctcgttg caaggcgtga
     8581 tggaacacgc ttcggaaatc aagggcaaag tgggcgagaa tctgcgtgcc gccctgccgc
     8641 gcctgccgct gtcgtatgat ttggtaacga ttaaaaccga tgtggacttg cacgccgagc
     8701 tttcagacgg catcgaaagc ctgcgccgta ccgcgccgaa atgggcgcaa ctggttgtcg
     8761 atttcaaacg ctggggcttc cgcacctggc tgaaagaagc ggaatcaaac atgaataccg
     8821 gctcgaccga tgatgtgttc ggcagcgaca gcatcggtga gcaggcggct ttgaatgcgg
     8881 aaatgccgtc tgaaaaacaa gctgaaaaag ccaccgcccc cgaaaggctg gattatcaag
     8941 ccgttaccac cgaagcgcag tttgccgctt tgttggacaa actgtcgcgg gcgaacacaa
     9001 tcggcatcga tacggaaacc acgtcattag acgcgatgaa cgcctcgctg gtcggcatca
     9061 gcatcgcttt ccaagcaggc gaagcggttt acatccccgt aggacacagc ctgaccgcag
     9121 cgcctgaaca gcttgattta caagacgtat taggccgtct gaaaccgcat ttgggaaacc
     9181 ccgccctaaa aaaaatcggg caaaacctca aatacgacca acacgttttc gccaactacg
     9241 gcatcgccct gaacggcatt gccggcgacg ccatgctcgc ttcctacatc atcgagagcc
     9301 atctcggaca cggcttggac gaattgtccg aacgctggct cggcttggaa accattacct
     9361 acgaatcgct gtgcggcaaa ggcgcgaagc aaatcggttt tgccgatgtc gccatcgggc
     9421 aggcgaccga atacgccgcc caagacgccg atttcgccct gcgcctcgaa gcgcacctgc
     9481 gcgcgcaaat ggacgaaaaa cagcttgaaa tgtatgaaaa aatggagctg cccgtcgcgc
     9541 aggtattgtt tgaaatggaa cgcaacggcg tgcaaatcga ccgcgccgaa ctcgcccgcc
     9601 aaagcgcgga actcggcgcc gagctgatga agctcgaaca acaagcctat gccgccgcag
     9661 accagccgtt caacctcaat tcgcccaaac agctgcaaga aatcctgttc gacaaaatgg
     9721 gcatccccac caaaggcctg aaaaaaaccg ccaaaggcgg catttccacc aacgaagccg
     9781 tgctcgaaca gctcgcgccc gactaccccc tgcctaaaat catcctgcaa aaccgcagcc
     9841 tggcgaagct caaatccacc tacaccgaca aactacccga aatgatttcc cccaaggacg
     9901 gccgcgtgca taccacctac gcccaagccg tcgccattac cggccgcctc gccagcaaca
     9961 accccaacct gcaaaacatc cccatccgca ccgccgaagg ccgccgcgtg cgccgcgcct
    10021 ttaccgcacc gcaaggcagc gtcatcgttt ccgccgacta ttcccaaatc gagctgcgca
    10081 ttatggcgca cctctccggc gacaaaaccc tgattgccgc gttccaaaac ggcgaagacg
    10141 tacaccgccg caccgccgcc gaagtgttcg gcactgcgcc cgaaaacgtc tcgtccgagc
    10201 aacgccgcta cgccaaaacc atcaacttcg gcttaattta cggtatgggg caatacggtt
    10261 tggcaaaatc attgggcatc gacaaccttt ccgccaaaaa ctttatcgac cgctacttcg
    10321 cccgctaccc cggcgtcgcc gaatacatgc agcgcaccaa agaacaagcc gccgcccaag
    10381 gctacgtcga aaccctgttc ggcagaaggc tctatttgcc cgacatccgc aacaaaaacg
    10441 ccaacgcccg cgcaggagcc gagcgcgccg ccatcaatgc ccccatgcaa ggcaccgcgt
    10501 ccgacctcat caaacgcgcc atgatagacg tgcgaaactg tctttcagac ggcatcggca
    10561 gcaaactggt catgcaggtg catgacgaac tggtgctgga agtcgttgaa accgaactgg
    10621 attttgtcaa agaaaaactg ccgcagatta tggcgaaagt ggacggcgga ttattggatg
    10681 taccgctggt ggctgaggtt ggcgtagggg agaattggga agaggcacat tgattgaaag
    10741 gtgttatatg ctatctttat ttaaataaaa tttaattttt ggtatatttt ttctaaatgt
    10801 tcctata
//
LOCUS       DAWFAD010000056        10568 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWFAD010000056 DAWFAD010000000
VERSION     DAWFAD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10568
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.056"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            153..1058
                     /locus_tag="WGO49_001093"
     CDS             153..1058
                     /locus_tag="WGO49_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0306209.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEIETVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
     gene            1104..1253
                     /locus_tag="WGO49_001094"
     CDS             1104..1253
                     /locus_tag="WGO49_001094"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306210.1"
                     /translation="MNWQAVKTGKTKHASNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="WGO49_001095"
     CDS             complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="WGO49_001095"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ0306211.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPASDIPDGTYRYPDDTDP
                     SEIHNTDMFHSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(2634..4427)
                     /gene="lepA"
                     /locus_tag="WGO49_001096"
     CDS             complement(2634..4427)
                     /gene="lepA"
                     /locus_tag="WGO49_001096"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ0306212.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(4570..5271)
                     /locus_tag="WGO49_001097"
     CDS             complement(4570..5271)
                     /locus_tag="WGO49_001097"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ0306213.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFTAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAADCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDDLLVGKAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            5457..6569
                     /locus_tag="WGO49_001098"
     CDS             5457..6569
                     /locus_tag="WGO49_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0306214.1"
                     /translation="MTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQDALKNADSAHDLRLAVQLRSRRAQSSGPDLELL"
     gene            6604..7581
                     /locus_tag="WGO49_001099"
     CDS             6604..7581
                     /locus_tag="WGO49_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ0306215.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKGIGKTEFARFAAQA
                     LLCENPSPDHRACGACASCHFFEQGSHPDFYEITPLSDESENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVVLIHPAESMNIQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            7585..7935
                     /locus_tag="WGO49_001100"
     CDS             7585..7935
                     /locus_tag="WGO49_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEZ0306216.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTDDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGSTIGGSRPTFTM"
     gene            7940..8719
                     /locus_tag="WGO49_001101"
     CDS             7940..8719
                     /locus_tag="WGO49_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0306217.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSVAEMVEAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDFGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRDQTLEQVAAYTTENFYRLFQKVPDMRAV"
     gene            8745..8960
                     /locus_tag="WGO49_001102"
     CDS             8745..8960
                     /locus_tag="WGO49_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ0306218.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            9061..9372
                     /gene="grxD"
                     /locus_tag="WGO49_001103"
     CDS             9061..9372
                     /gene="grxD"
                     /locus_tag="WGO49_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ0306219.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            9478..10104
                     /gene="upp"
                     /locus_tag="WGO49_001104"
     CDS             9478..10104
                     /gene="upp"
                     /locus_tag="WGO49_001104"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ0306220.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            10127..10447
                     /locus_tag="WGO49_001105"
     CDS             10127..10447
                     /locus_tag="WGO49_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ0306221.1"
                     /translation="MNFQDYLATFPSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
BASE COUNT         2484 a         2693 c         2822 g         2569 t
ORIGIN      
        1 gggttttatc cggcaggacg gttttttgcc ccaacggaaa atagtcggcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcagatcc gtccccgtcg gttcgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttag cacggttttt caggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt tctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tctttttcgg cgagcgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg tcagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gacggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgc gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtccg agccttgact ccgtccggca tcagattttc
     1081 tgccgtggca aacctgcgta cgattgaact ggcaggcagt caaaaccggc aagaccaaac
     1141 acgccagcaa cggcgcggag cggaaaatgc cgtctgaagc cctttcagac ggcatcgggg
     1201 cgggcagaat caaaaaagcc gcctccgctg gaatggcaat gcaactaaaa taatgtccgt
     1261 atttccatat gccgcccaca ccgtcagtcg gacgaaatgc cctttattca atgggatggg
     1321 tttccacaca aacggaaata tagtggatta aatttaatcc actatattat ccccgaggct
     1381 gccggcaggg aaacaaggcg tttgatgata aaaatgccgt ctgaaacagg gttcggatgg
     1441 catttttgat gttccggcgt aattttccgg cctaacggat tgccgtaccg gccctgccga
     1501 aatcgccgaa gttcatcaaa atgaacattg ccttgccgac aaccagcttg tcatccacaa
     1561 atccccagta gcgcgaatcg gcactgttgt cgcggttgtc gcccatagcg aaatagcgtc
     1621 cttcgggaac tttgcacacg aaaccgctgc cgtcgtcggc atattggcag tgttccaaac
     1681 cgctttgctc tatggaatat ccgttttcag acataatatc ggaggtatat ttgcccaata
     1741 cgggcaggga aacggcaggc tgtccttctt ttttcagaat attgaaggat ttgccgtcca
     1801 aaccgctgtg gaacatatcc gtattgtgga tttcggatgg gtcggtgtcg tcgggataac
     1861 ggtatgtgcc gtcaggaata tcggaagcag gttttccgtt taccgtcaaa actttattcc
     1921 gatattcgac cacatcgccc ggaatgccga caatacgctt gatgtaggtc atctccggtt
     1981 gcaaaggata attaaaaacg accacatcgc cccgttcgat tttgcctgta ggaataaata
     2041 tattgtttaa aacgggtacg cgcaggccgt aggaaaattt gccgaccaaa atgaaatcgc
     2101 ccttaatcag gcccgggcgc atcgagctgg acgggatttg gaacggttcg gcaataaacg
     2161 accggatgag gaacaatacc aaaacggtag ggaagaaact gccgaaataa tcgccgaagt
     2221 ggctgctttc cgagatttcg ggatgagtct tcaggcggta tttgtatacc ccccaagccg
     2281 taccgcacaa tacaacgaaa atcaggaaaa cggcggtaaa gctcataaac agggacaaag
     2341 cggcaaacac gccgaccgcc gtcaggatat aggcgtattc cagaccggaa ctccattcac
     2401 cgttttcctg ccgctttttg tcgcttttga aataaaggat gatgccggca agcagcgcgg
     2461 cagccgcgcc cgacattagc attgtgttca ttgttgttcc ttaatgctta aaaacccgcc
     2521 tgtccgtgca accgttttaa ggcggcaaat tgcaaaattt gtttgcgggc gtgtgcccct
     2581 gaaatcaggg cggtttgagg ggtgttcccg acgcgccgcc ctgtgtgcca gagttatttg
     2641 tcgctcacct gcaaaatcgc caagaacgcg ctttgcggaa tttccacatt gcccacttgt
     2701 ttcatacggc gtttacctgc cttttgtttt tcaagcagtt ttttcttacg cgtaatatcg
     2761 ccgccgtaac atttcgccaa aacgttttta cgcagggctt tgacgttttc gcgggcgata
     2821 atctggctgc cgatggcggc ttggacggcg atgtcgaaca tttggcgcgg aatcagctcg
     2881 cgcatttttg atgccagctc gcggcctcgg tgaaccgcgc tttgacggtg cacaatcagg
     2941 cttaatgcat cgactttttc gccgttgacc ataatatcca gcttaatcaa atcagacggt
     3001 tggaactctt tgaaatggta gtccaacgaa gcataaccgc gcgaagtgga tttgagtttg
     3061 tcgaagaagt ccatgacgac ttcgttcatg ggcaaatcgt aagtcagcat gacttggcgg
     3121 cccatgtact gcatattgac ctgaacgccg cgcttttggt tacacaaagt catgacgttg
     3181 ccgacgtatt cctgcggcac aaggatggtc gcggtaataa tcggctcgag tatggtttcg
     3241 atgctgccga tgtcgggcag tttggacggg ttttcaactt cgattttttc gccgcttttc
     3301 aacacgactt cgtaaaccac cgtcggcgcg gtggtaatca aatccatatc gaactcgcgc
     3361 tccaagcgtt cctgcacgat ttccaagtgc aacagaccca agaagccgca acggaagccg
     3421 aagcccaatg cctgggaaac ttcaggctcg aatttcaacg aagcgtcgtt aagctgcaat
     3481 ttttccaaag cgtcgcgcaa ggcttcgtag tcgtggcttt ccacgggata aagtccggcg
     3541 aatacttggc tttgtacttc ttggaagccg ggcagcggct cagtggcagg gttggcaacc
     3601 agtgtaaccg tatcgccgac tttcgcctga cccaattctt ttacgccggt aatcaaaaag
     3661 cccacttcgc cggctttgag ttcttgtttt tgaaccgatt tcggtgtgaa tacgcccaac
     3721 tgctcaacct gcgtttccgc cttggtgctc ataaagcgca ctttgtcttt cagtttgatg
     3781 gtgccgtttt tcactcgaat cagcatgacc acgccgacgt agttgtcaaa ccacgaatcg
     3841 acaatcacgg cttgcagcgg cgcgttttca tcgccggtcg gcgcaggaat tttggcaacg
     3901 atttcttcca aaacgtcttc cacgccgatg ccgcttttgg cggaacactg caccgcgccg
     3961 accgcatcga tgccgatgat gtcttcaatt tcctgctcca cgcgttcggg gtcggcggct
     4021 ggcaggtcga ttttgttcaa aacaggcaca acctccacgc ccaaatcaat cgcggtgtaa
     4081 cagttcgcta cggtttgcgc ttccacgccc tgcgacgcgt caacgaccaa aagcgcgcct
     4141 tcgcaagccg acagcgaacg ggaaacttcg taagagaagt cgacgtgtcc cggcgtgtca
     4201 atcaaattaa gctgatacac ctgcccgtcg cgcgctttat agttgagcgc ggcggtttgc
     4261 gctttgatgg taatgccgcg ctctttttcg atgtccatgg aatcgagtac ctgcgtactc
     4321 atttcgcgca aatccaaacc gccgcaatat tggatgaagc ggtcggcaag cgtcgatttg
     4381 ccgtggtcga tgtgggcaat gatggagaaa tttcggatat ttttcattag agttgttttg
     4441 aatgtcggac agtgggtttg ggaaatgccg tctgaacaaa cggcgttgcg tccgaatatc
     4501 gggcgcaacg tggaaatagc ccgatattct aacggaaaac cgctgttttg gcatgatttg
     4561 ataaaggttt tataaagatt tgacgatttc tgccaccatt tttgcagaac ttgccgccgc
     4621 cgttttcaaa aattcgtcaa agctgatgtc tgctttttca tctgccgaat cggaaaccgc
     4681 gcggatgatg acgaaaggcg tttccaactg atgacaggtt tgggcgattg ccgccgcttc
     4741 catttctacc gccttaactt cggggaagtg cttgcggatt tccgccacgc cttcgctgct
     4801 gtggacgaag cggtcgccgc tgacaatcag accttgttct accgccgcgc cttcaaacgt
     4861 ccgcgccgcc cgttttgcct tgccgaccaa gaggtcgtct gaaacgaata cggcgggcag
     4921 ttgcggcact tgtccccaaa catagccgaa tgcggttaca tcgacatcgt ggtgtgcggt
     4981 ttccgtgcca atcaccacgt cgccgacttt caaacccttg cccaaaccgc ccgcgctgcc
     5041 ggtgttgatg acgcagtccg ctgcgaattc acggataatc caagccgttg caaccgccgc
     5101 gttgaccttg ccgatgccgc tcaatgcaag caccatgcgt tttcccgcta attcgccttc
     5161 ataggcggta aacttgccga aagagacggc tttgacattt tccatcatct cgcgcaaaag
     5221 ctcgatttct tgttccattg cgccgataac ggctactgtt ttcaaagaca tattgctgac
     5281 ctgttgtgaa tttcggatag aatgcctgat tatacacgct aacacggcag gattgagtgg
     5341 gggcggtttg tccgtgccgt ctgaaacggt ttcagacggc acggcgggtt tttggtagaa
     5401 tgggaaggta cagattgttt gaagattagg ggacgaggat gtttaccgat gaaaatatga
     5461 ccgcaaagga agaactgttc gcatggctgc gccatatgaa ccaaaacaaa ggttccgacc
     5521 tgttcgtgac gactcatttc ccgcccgcaa tgaagctgga cggcaaaatc acccgcatca
     5581 cggacgaacc gctgacggcg gaaaaatgta tggaaatcgc cttttcgatt atgagtgcga
     5641 agcaggcgga agaattttca tcgaccaacg agtgcaactt cgccatcagt ctgccggaca
     5701 ccagccgctt ccgcgtcaat gcgatgatac agcgcggcgc gacagcgttg gtattccgtg
     5761 cgattaccag caagattccc aagtttgaaa gcctgaacct gccgccggtc ttgaaggatg
     5821 tcgcgctgaa aaaacgcggg ctggttattt ttgtcggcgg caccggctcg ggcaaatcga
     5881 cttcgcttgc ctcgcttatc gactaccgca atgaaaattc gttcggacac atcatcacca
     5941 tcgaagaccc gatcgagttt gtccacgaac acaaaaactg catcatcacc cagcgcgagg
     6001 tcggcgtgga tacggaaaac tggatggcgg cgttgaaaaa cacgctgcgt caggcgccgg
     6061 atgtgattct gatcggcgaa atccgcgacc gcgaaacgat ggactacgcc attgcctttg
     6121 ccgaaacggg gcatttgtgt atggcgacgc tgcacgccaa cagcaccaat caggcactcg
     6181 accgcatcat caactttttc cccgaggagc ggcgcgaaca attgctgacg gatttgtcgc
     6241 tcaaccttca ggcgtttatt tcgcaacgcc tcgttccgcg agacggcggc aagggcaggg
     6301 tggcggcagt cgaggtgctg ctcaattcgc ccctgatttc ggagttgatt cacaacggca
     6361 acatccatga aatcaaagaa gtgatgaaaa aatccactac cctgggtatg cagaccttcg
     6421 accaacacct ttaccaattg tatgaaaaag gcgatatttc cttgcaggat gccttgaaaa
     6481 atgccgattc ggcgcatgat ttgcgtttgg ctgttcagtt gagaagccgc cgcgcgcaaa
     6541 gttccggacc ggatttggaa ttgctctgat gaagggtatg gatttccgga cggatggttt
     6601 gaaatgattt atccgtggca taatgagcaa tggcggcaga ttgcggaaca ttgggagcgc
     6661 cgtcccaatg cttggctgtt tgccggcaaa aaaggcatag ggaaaaccga gtttgcccgc
     6721 tttgcggcgc aagcattatt gtgtgaaaac ccaagtccgg atcaccgggc ttgcggggct
     6781 tgtgcttctt gtcatttttt tgaacaaggc agtcatccgg atttttacga aatcactccc
     6841 ttgtcggacg aatccgaaaa cgggcgcaaa ctgttgcaga tcaaaatcga tgccgtcagg
     6901 gaaatcatcg ataatgtgta cctgacttcg gtacggggcg gtttgcgcgt ggttctgatt
     6961 catcctgcgg aaagtatgaa tatccaggcc gccaacagtt tgttgaaagt gttggaagag
     7021 cctccgccac aagtggtctt tttgctggtc agccacgcgg cggacaaggt tttaccgacc
     7081 attaaaagcc gctgccggaa aatggttttg cccgcgcctt cccatgaaga ggcattggca
     7141 tatctgcgtg aaaggggtgt ggcggaacct gaggaacgtc tggctttcca ttccggagcg
     7201 ccgctgtttg atgaggcgga cggtgtccgt gcgttgcgga ttaaactgtt ggatattttg
     7261 gcagaaccga ggttgttgaa gattttggat tacgccgcgc ttttcgataa ggaaaaactt
     7321 ccgctcgccg tatttgtcgg gtggatgcag aaatggctgg tcgatttggg attgtgcctg
     7381 caacacatga aacccgtcta ttatcccgct tatgaagaca ggctgcttca gacggcatcc
     7441 ggtttccgcc cgcgcaatgt atttgcggcg gaggatatgc tcaaacagct tgccccctac
     7501 gggtttcata ctttaaatgt caaaatgcag atcgagcatc tgctcatcaa ttatttggaa
     7561 ttgaagaaag agaacgggtg aattatgtca gacggacaaa atattccggc aaaaatgatg
     7621 tcgttgcagc tgaaagacat gaatctgctg tacagctcct atatgccgtt tttggaacat
     7681 ggcggcctgt ttgtgcagac cgacgacgta ttttccatcg gggacgatat tctgcttgcc
     7741 gtagaaatcc tcaacttccc caaactgttc ctgccaacca aggtcgcttg gattaatcct
     7801 gcgcgtactt cctccaaacc caaaggggtt gggctggcat tcacaaaaca cgaaaactgc
     7861 ctgaaagtca aagaccagat cgaagtcgaa ctgggcagca caatcggcgg cagcagacct
     7921 acgtttacca tgtaacgcca tgcatatcat cgattcgcac tgccacctca attttgaagg
     7981 tttgaaagaa cgcctgcccg aagttttgtc caacatggaa gcaaacggcg tggggcaggc
     8041 gctcgccatc agcgtcagta gggaaagctt ctccgaagtc tttgccatcg ccgaagcgca
     8101 cgaacacatc tattgcacca taggcgtaca tcccgacagt aaggaagccg aagaattttc
     8161 cgtcgcggaa atggtcgaag ccgccgccca tccgaaagtg gtcggcattg gcgagacggg
     8221 tttggattat tactggtgca aaggcgattt gtcctggcag cacaaacgct ttgcagacca
     8281 catcgaagca gccaatcaaa ccggactgcc cgttatcgtc catacgcgtg atgcggcggc
     8341 ggataccttg tctatcctga aagaatgccg ggttaattcg ggcgttatcc actgtttttc
     8401 cgaagacatc ggttttgccc gtgcagcaat ggattttggg ctttatattt ctttctcggg
     8461 aatcgttacc tttaaaaacg cacccttggt tcaggaggcg gcaaaatacg ttccggacga
     8521 ccgcattttg gtggaaaccg atgcgccgtt cctcgccccc gttcccaaac gcggcaggca
     8581 gaacgaaccg gcttttgtgc gccataccgc cgaacatatc gccaaattgc gggaccaaac
     8641 attggaacag gttgcggcat atacgacgga aaacttttac cggctgtttc aaaaagtacc
     8701 cgatatgcgg gccgtctgac cctgtgccga cgataaggaa aaccatgaag gcaattcatc
     8761 cgtatgcatg tccgcgctgc tgccggctgc ctgccaacac gtttcggaca ggcatggcaa
     8821 attccgcttc caaattctgc attgccaaag gcggcagacg ggaagtaaaa aaagacgaaa
     8881 gcggcggcgg atatgccctg tgccatttgc cggacagtag gattgtcgag gagtgggaat
     8941 atttccgttc acaacattga tactgcgcga tatacggcaa atattgtggg aagtttccgc
     9001 ttttgcgtat aatgcgccct acctgacaaa ttttgtcaac tttatcaaaa ggaataagcg
     9061 atggcttcca tccacgacca aattaaagaa gtagtaacga cacaccgcgt cgtattgttt
     9121 atgaaaggta cgaagcagtt tccgcaatgc ggtttctctt cccgcgccgt gcaaatcctg
     9181 aacgcggcgg gctgcaccga ttacgttacc gtcaacgtat tggaaaatcc cgaagtacgc
     9241 caaggcatta aggaatacag cgactggccg accatccccc aactttatgt gaacggcgag
     9301 tttgtcggcg gttcggacat cctgatggaa atgtatgagg caggcgagct gcaagagctg
     9361 ctgaaagcct gatggattcg gcaatgccgt ctgaacgtgt ttcagacggc attttctttt
     9421 ccggcaaatc aaaaaaaagt ataatggcgc gtctcaaaat cacactggaa caccgcgatg
     9481 aacgttaatg ttatcaacca tccgctcgtc cgccacaaat taaccctgat gagggaggcg
     9541 gattgcagca cctacaaatt ccggacgctt gccatcgagc tggcgcgcct gatggcatac
     9601 gaggcaagcc gtgattttga aatcgaaaaa taccttatcg acggatggtg cggtcagatt
     9661 gaaggcgatc gcatcaaggg caaaacattg accgtcgttc ccatcctgcg tgcaggtttg
     9721 ggtatgcttg acggcgtact cgacctgatt ccgactgcca aaatcagcgt agtcggactg
     9781 cagcgcgacg aggaaacgct gaaacccgtt tcctacttcg agaaattcgt ggacagcatg
     9841 gacgaacgtc ctgccctgat tatcgacccg atgctggcga cgggcggttc gatggttgcc
     9901 accatcgacc ttttgaaagc caagggctgc aaaaacatca aggcactggt gctggttgcc
     9961 gcgcccgagg gtgtgaaggc ggtcaacgac gcgcatcccg acgttacgat ttacaccgcc
    10021 gcgctcgaca gccacttgaa cgagaacggc tacatcatcc ccggcttggg cgatgcgggc
    10081 gacaagattt tcggcacgcg ctaactgact gattttcgga gttgatatga attttcaaga
    10141 ctatctcgcc acatttcctt caatcgacca tctgggcggt ttggacgttc aggatgccga
    10201 cggcaaaacg gttcaccaca ttcccgccgt tcagggtaag ctcggttcgc tcaagctgta
    10261 caatgctttg gcagaacgtt ttgacggaaa attggataaa gaagcggcag aacagggttt
    10321 gatatggttt gccgaacacg ttgccgacgc gcgcgcccat cccggcaagc atcccaatat
    10381 cgacctgctt gaaaaagttg tgcaaagcgg cgaaaccttg ctgctcaagc cgcttgccgc
    10441 gcaataattt tcgaccatgc cgtctgaaat ccgtttcaga cggcatttta tcggaaagaa
    10501 gaccgtaaaa cgggcatttt cttttctatt tcaggatacg ggcaatgatg tttcaacaca
    10561 caggacga
//
LOCUS       DAWFAD010000057        10508 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWFAD010000057 DAWFAD010000000
VERSION     DAWFAD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.057"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            185..367
                     /locus_tag="WGO49_001106"
     CDS             185..367
                     /locus_tag="WGO49_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306222.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(429..1430)
                     /locus_tag="WGO49_001107"
     CDS             complement(429..1430)
                     /locus_tag="WGO49_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ0306223.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1507..5340)
                     /locus_tag="WGO49_001108"
     CDS             complement(1507..5340)
                     /locus_tag="WGO49_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ0306224.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEVAPLVRALIELDLVIIPRGGGTGYTGGAIPLDAMSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVAAVKERWDWLLANLDTPLADYKARYGAA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLAEIFSGKTDTKIIQGLGKIHAKTV
                     RGRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVQGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYTDDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5601..5697
                     /gene="ffs"
                     /locus_tag="WGO49_001109"
     ncRNA           5601..5697
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WGO49_001109"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5865..7556
                     /gene="dld"
                     /locus_tag="WGO49_001110"
     CDS             5865..7556
                     /gene="dld"
                     /locus_tag="WGO49_001110"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ0306225.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGY
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGVGKTSKKKNWAE"
     gene            7688..7795
                     /locus_tag="WGO49_001111"
                     /pseudo
     CDS             7688..7795
                     /locus_tag="WGO49_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7851..8222
                     /locus_tag="WGO49_001112"
     CDS             7851..8222
                     /locus_tag="WGO49_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ0306226.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8377..8925
                     /locus_tag="WGO49_001113"
     CDS             8377..8925
                     /locus_tag="WGO49_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0306227.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDDELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            9060..10151
                     /locus_tag="WGO49_001114"
     CDS             9060..10151
                     /locus_tag="WGO49_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0306228.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
BASE COUNT         2437 a         2884 c         2842 g         2345 t
ORIGIN      
        1 gaaatcccct aaatgtctta gtaggaattt aggggatttt gaggaatttt gcaaaggtct
       61 ctatttgtac tgtcttcggc tcgccgcctt gtccttattt aaatttaatt cactatattt
      121 gcccgtgcgt ttgtgataaa ttcggcactg tatttgatat gattttaaca aatcgggagg
      181 ggcaatgttt tggtatgtaa tcggcttttg ctcctttgtc gtcgcgctgc tgtcgctgtg
      241 ggtcaatgtc ggcgcattcg gtatgcagga agacgatacc ccgcaatcgg attatgaaaa
      301 acgtttgggc ttgggtgcga agctgaaaaa caaaaatacg ccggatacgg aagaaaagca
      361 gcaataaaga tatgaaatgc cgtctgaaag attttcagac ggcatttgct ttgaagcatg
      421 ggagacagtc aaacatttat ctcatgctca aatgcgttca ggattcggcg gacttcatcc
      481 gcatccttaa ccgttcggac gcggtcaaac agcgtttgcg ccgggtcgaa cgtttttttc
      541 atcatgccca accattgctt aagacgggcg atcgggtatt tattgtttgc ctcttttgtc
      601 aggcacagct cgaaaaactg ccgtatccat ttagaaactt cggcaaaatc cgtgtctttg
      661 accggcccgc cgttctcgta ttgcttgatt tgccgcgcca aatcggggcg gatgaccgcg
      721 ccgcgaccga gcatcacgct gttgcagccg ctgattgttt tgatgccgat atagtcttgc
      781 aggctgaaaa cgtcgccgtt ggcggtaacg ggaatattga cgctgtctcg gattttcctt
      841 atccattccc aatgcgccgg cggttcgtaa ccctcggctt tggtacgcgc gtgtacggtc
      901 agtccgcatg cgcccccttc ggcaatcgca caggcgcatt ccaaagcccg gcttttgtct
      961 tcgtaaccga gccgcatttt tgcggtgagc ggaatatgtg cgggcaaacg tccgcgcagc
     1021 gttttgacga tgtggaatat cagttccggc tcttttaaaa ggattgcgcc gcctttgtgt
     1081 ttgttgacgg tgggcgcggg gcagccgaag ttcaaatcga ttttgttcgc accgaagcgg
     1141 acggcttcca gcgcattcgc cgccatattg tccgcatcgc tgcccaaaag ttggacggtg
     1201 cagggcgtgc cggaaaacgt tttgtttccg ttggcgattt cggggacata ttttaaccat
     1261 atggatcggg aatgcacggt atgggtaatg cgtacaaatt cgctgacgca ttcgtcgtag
     1321 ccgccaatac gcgtcagcag gtcgcgcatc acgtcatcga ccagaccctg cataggggca
     1381 aggatgattc gggttttttg tttgggttcg gttgtttgtc cgtcaatcat gattcgggta
     1441 ggtatttgat tcaaacaatg ccgtctgaag gctttcggcg ttcagacggc atttccgtgt
     1501 ccgttgttac agcaatactt tttccacgcc gccgttgttg gcttttttca caaactcgtc
     1561 tagccagttt tcgccgagga tgtgtttcgc catttcgatg acgatgtagt cggcaggcat
     1621 attgttgtcg tcggtgtagc ggctcaagcc ttgcaggcag gctgggcagg aagtaagcat
     1681 tttgacgggt tcaccctgcg gcagctcttt gaggtttttc tcgatttcct cttgtttgcg
     1741 gaatttgact tgggtggcga tatcgggtcg tttgacggcg aacataccgg actcgccgca
     1801 gcagcggtcg cttaagacga ctttctgccc catcaggctg ctggccattt gggtggcgtt
     1861 catggttttg atgggggtgt ggcaggggtc gtggtagagg tattgctgac cttgcacgcc
     1921 gtcgagtttc acgccttttt cgagcaggta ttcgtggatg tcgatgatgc ggcagccggg
     1981 gaagatttct tcaaagcggt atttttcgag ctggtcgtaa caagtgccgc aactgacaac
     2041 gacggtctta atgtcgaggt agttgagggt gtttgccata cggtggaaag ccacgcggtt
     2101 gttggtgctc atttcttcgg ctttagcctt gttgccgccc gcgtcttgcg gatagccgca
     2161 acacatatag ccgggcggca ggacggtttg tacgccgacg tgccagagca tggcttgaac
     2221 ggcaagtccg atttggctga acaggcgttc cgaaccgcaa cccgggaagt agaacacggc
     2281 ttcggcatct tcgggcgcgg cggggttgcg gatgatgggg atgcttttgc cgtcttcgat
     2341 gcccaataag gagcgcggtg ttttggcggg tacgcttttc ggcaaaggac ggttgatgaa
     2401 atggataacc tgttctttaa ttggggcttt gccgacggtt gccttgggtt cggctttttg
     2461 ctttttcgtg ccgatcggaa gaagcttgcc gattttgtag gcaaagtttt gcgctgggaa
     2521 gccggtctgt atcatggcgg cgcgaagggc tttgatggtt ttcgggccgg tggcgttcaa
     2581 aaacgccata cccatagctg cggcaggcgc aaatcgtttg tggccggaat cggcaaggta
     2641 gttgcggacg gctacggtta cgtcgccgaa gtcgatgttg acggggcagg gtttgacgca
     2701 gcggtggcac acggtgcagt ggtcgccgat gtccatgagt tcttcaaagt gtttgacgga
     2761 aacgccgcgg cgggtttgtt cttcgtataa gaaggcttcg gtcagcaagc ccacgccgag
     2821 gattttgttg cgcggactgt acagcaggtt ggcacgcgga acgtgagtag agcagacggg
     2881 tttgcatttg ccgcagcgca ggcagtcttt gacggaatcg gcaatcgtgc cgaggtctga
     2941 tttttccata atcagcgatt ccgcgcccaa aagctcgaaa gacggcgtgt aggcgttgcg
     3001 taagtccgag cctttcatca gtttgtggcg gttgaaggtg tgtttgggat cgacttggtt
     3061 tttgtagtcc caaaacggct gcatttcttc atcgcttaaa aattcgagct tggtgatgcc
     3121 gatgccgtgt tcgccggaaa ttacgccgcc gagcgaacgg gcgattttca taatgcgttc
     3181 gaccgaacgg taggctgtct gaagcatttc ggcatcgtct gagttgaccg gaatattggt
     3241 gtggacattg ccgtcgccgg cgtgcatatg cagggcgaca aagactctgc cgcgtacggt
     3301 tttggcgtgg attttgccca agccttggat aatcttggta tcggttttgc cgctgaagat
     3361 ttcggcgagt ggtttcatta cgtctgcttt gacagacacg cgcaggcgga aatcgcggaa
     3421 tgcggtaaag cagctttcat tgtctttggc ttcgggcgcg gcgtggacgg ctgcaccgta
     3481 gcgggctttg tagtcggcaa gcggcgtgtc caaattggcg agcaaccagt cccaacgttc
     3541 tttgacggcg gcaacgtggg ccaaggcgtg tttgccgcgt tcgcccaaaa gttcggcggt
     3601 cggcaggtca gtgcccattt tgtcgatggg gagtttgccc gaaagatatt gctccaaggc
     3661 ggcacagagt ttgagcttgt tttggatgga aagctcaatg ttgatgcgtt cgatgccgtc
     3721 cgaatactcg ccgagccttt cgagcgggat gaccacgtct tcgttaattt taaaggcgtt
     3781 ggtgtgcttg gcgatggcgg cggtgcggct gcggtcgagc caaaaggttt tgcgcgcttc
     3841 gggagacacg gcgataaagc cttcgccatc gcgggcgcgg gcaagttcgc aaatgtgttc
     3901 ggcggctgcc tctacggcgg cttcgtcgtc ggaaaccacg tccgccagca agaccatttt
     3961 cggccgtccc ttgcccgccg ctttggttgc gtagccgacg gcgcggacgt aacgccagtc
     4021 caaatgttcc aaacctgcca gccgaacgct gtcgtgggcg agcaggaaat cgcggatttc
     4081 gacgatagaa ggcgtagcgg tggcgaccgt gccgaaaaac tccatacaca cagtgcgcgt
     4141 gtatttcggc attttgtgca acacgaaagc aacgccggtg atgatgccgt ccgtgccttc
     4201 tttctgtacg ccgggcaggc cgctcaagaa tttgtcggta acgtctttgc ccaaaccgac
     4261 tttgcgaaat ttgtggccgg gaatttccaa acgttcggtt ttaacgatat tgaggccgtc
     4321 tgaatccagc gtatgtacgt caaacacggc ggtttcttca tcgtggattt tgccgaaatt
     4381 gtggcgcacg cgctcgatac gcagccattc gccttgaggg ttgaccatct gccagtaggc
     4441 gaggttgtcc aacgccgtcc cccacaacac ggcttttttg ccgcccgcgt tcatcgccac
     4501 attgccgccc acgcaggacg catcggcaga agtcggatcg acggcgaaca ccaaacctgc
     4561 ctgatgcgcg gtttcttcca cccgccgcgt aaccacgccc gcgccgcacc ggataatcgg
     4621 atgcttgccg tccaatcctg ccagctcaac gtattccacg ccgcgatgct tgtcgagctt
     4681 ttcggtattg atgaccgcgc tcattgcgtc caaaggaatc gcgcctccgg tataacccgt
     4741 gccgccgccg cgcgggataa taaccaagtc caattcgatt aaagcgcgca ccaaaggcgc
     4801 gacttccgcc tccgtgtcgg gattgacgac gacaaacgga tactccacgc gccagtcggt
     4861 tgcatcggta acgtgcgtta cccgcgccag cccgtcgaac ataatattgt gcggcttggt
     4921 gattttgctc aaacgctcca aaatctgccg ccgtttttgg ctggtttcat caaaactgct
     4981 atcaaaacgc tcgactgctt tttctgctgc ggcaaccaac acatccactt gccgattatc
     5041 gtcgcggcgt ttgcggattt catttaagcg gtggcgcatt tcacgtacca gcgcggcgcg
     5101 gcgttttggg tgctccagca agtcatcgac cagatacgga ttgcgcacga ccacccaaat
     5161 atcgcccaac acttcaaaca gcatccgcgc cgaacgcccg gttttgcgct gtccgcgcaa
     5221 gtcttgcagg atttgccacg cctcatcgcc cagtaggcgg atgacgattt cgcggtcggt
     5281 gtaggaagta tagttgtagg gaatttcccg aatacgctga ggggcggtag tcgtggtcat
     5341 ggtgtgtccg tgtttggcag tttttatttg aatgccgtct gaaaattcgg tttcaggcgg
     5401 gtttgaaaag gcgataatgt agttcaattt ttgatatttt tcaatgccga aacaggaaat
     5461 acccttatag aaacaaaata acgctttgag aaattgttga caatattatc aaatataaat
     5521 tttaggtggt gtttccggcg tatgaccgta tcaaaacggt tgagtttttc agaaaacccg
     5581 ttataatgcc gtttctctga ggcgggtctc cccgcatggc aaatcggaac accgggtcag
     5641 gggcggaagc cagcagccca ctccgatgcg ccagtgccgg gggtttggcc cgccgcccta
     5701 tttgaaacgc cggagctttg atgttccggc gttcccgtat cgcaccgttg ccgtctgaaa
     5761 gccgttcaga cggcattttc tttactttgc cataacgtat aactacaatg tgaaaacaat
     5821 tccaatgtgc atatgcacct gccgacaatt ttaaggaaac tgccatgagc gcatcacaat
     5881 tactgtcaag actgacccaa accgtaggcg aaaaatacat catcaccgac cccgcgaaaa
     5941 ccgaacaata ccgccaaggc taccgcttcg gcgagggtaa ggcgttggcg gtggttcgcc
     6001 ccggaagcat tctggaaatg tggaaaattc tgcaggcgtg cgtcgaagca gacgtgattg
     6061 tgattacgca ggcggcgaat accggactga ccggcggttc gacccccgac ggcaacgatt
     6121 acgaccgcga catcgtgatt gtgaacacca tgcggatgaa catcatccaa accatcaaca
     6181 acaacgaaca agtcgtctgt ctgcccggtt cgaccctgaa ccagcttgaa ttgctgctga
     6241 aacctttggg gcgcgaaccg cattcggtca tcggctcatc ctgtatcggc gcgtccgtct
     6301 tgggcggcgt gtgcaacaac tccggcggcg cgttggtaca gcgcggcccg gcctacaccg
     6361 aaatggcgtt gttcgcccaa atcaacgaag aaggcaggtt ggaactggtc aaccacttgg
     6421 gcatagactt gggcgacacg cccgaagaaa tcctgaccaa cctgcaaggt catcattatc
     6481 aaaacaaaga catcaaacaa gacgcgggca aaggatacga ccacgcctat tgcgaacacg
     6541 tccgccaagt ggacgaaccg accgccgcgc gtttcaatgc cgaccccgcc cgccattacg
     6601 aagcctcggg ctgcgcgggc aaactgatgg tttttgccgt ccgtttggac accttcccgc
     6661 aagaaaaaca aaccgccgtg ttctacatcg gcacgaacga catcaacgag ctgaccgaca
     6721 tccgccgcgc cgccttgggc gaatttgaaa gcctgcctgt ttccggcgaa tacatccacc
     6781 gccacgcttt cgatattgcc gacgtgtacg gcaaagacac gttttacgtc atcaaaaaat
     6841 tcggtacgca ccaactgccg aaattatttg acttgaaggc gcgcgtggac aggttcggca
     6901 aaaaagccag cttcctgccc aaacattttt ccgacaaggc catgcagttc gtcagcaaat
     6961 acctgcccga ccacctgccc aaatccatgc gcgattaccg cgacaaatac gaacaccacc
     7021 tgattctgaa aatgggcggg aaaggcgtgg atgaggcgcg cgcgttctta aaagaatatt
     7081 ttgcacacca cagcggcgcg tttttcgagt gcaacgccga agaaacccaa gccgcgatgc
     7141 tgcaccgttt cgccgtcgcc tccgccgcca tccgctaccg cgccgtgcat gatgacgaag
     7201 tggaagactt ggtcgcgctc gacatcgccc tgcgccgcga cgatcgcgac tggtttgaaa
     7261 aactgccgtc ggaaatcgac aataaaatca tccataaact ctactatgga catttcatgt
     7321 gccacgtttt ccatcaggat tacatcatca aaaaaggcaa cgactgcatg gcgttggaac
     7381 acgaaatgct gcatctttta gaccaacgcg gcgcgcaata tcccgccgaa cacaacgtcg
     7441 gtcatttgta tgaagccaaa cccgcgctca aacagtttta ccgcaaactt gacccgacca
     7501 acagcttcaa cccgggcgta ggcaaaacca gcaaaaagaa aaactgggcg gaataagcgc
     7561 gtccgctttg aaggcaggca atgccgtctg aaggcaaaac gccgttcaga cggcattgtc
     7621 gggtttgaag ttccccatcg ggtaatcgct ttcaagccgt atgccggcac aggcgcgcgc
     7681 gcctcccaaa tatagtggat taaatttaaa tcaggacaag gcaacgaagc cgcagacagt
     7741 acagatagta cggcaaggcg aggtaacgcc gtactggttt aaatttaatc cactataaag
     7801 tctgatccta ccttcccaaa gggcagggtt tcaaccgaaa aggaaacacg atgaaaccat
     7861 acaaaatcta cacccatccc gccctgccgc cccaagccgt caaacaaggc tggtcgtggc
     7921 cgggcctttt gttcggcacg ctgtgggcgt gtttcaaaag aatgtggggt ttggggctgg
     7981 ggctgacggg cgcgatattc gtgctggcgg tgtttgccca actggtttac ggcgacacgc
     8041 ccgccacaga ctcggcgttt aacgtattgg gcttggcggt ttccgtctgg ttcggcgcaa
     8101 agggcaacag cctttatgca cgccaccttt tatcgcgcgg ctataccgaa ttgcccgaaa
     8161 cggttgaggc agccaatccc caagccgcat tggcgcaata tttcgggcgc gggggccgat
     8221 aagcgcgttt tcttttccat catgccgtct gaaaaatgtt cagacggcat ttcttataac
     8281 cttttgtttg cagcacataa ccaatcagtc tttcctattc cgttccgaaa tgcataccat
     8341 gcgcccgtac ccaatatttg cgaacaagga aagaaaatgg cacgtttaac cgtacacacc
     8401 ctcgaaaccg cccccgaagc cgccaaagcg cgcgtcgagg cggtacttca aaacaacggc
     8461 tttatcccca accttatcgg cgtattatca aacgcccccg aagccttggc gttttaccaa
     8521 gaagtcggca agctcaacgc cgccaacagc ctgaccgctg gcgaagtcga agtcatccag
     8581 atcatcgccg cccgtaccaa ccaatgcggt ttttgcgtgg cagggcacac caaactcgca
     8641 accctgaaaa aactcctgtc cgaacaatcc gtcaaagccg cgcgcgcttt ggcagcaggc
     8701 gaatttgacg atgccaaact cggcgcgctc gccgccttca cccaagccgt aatggcgaaa
     8761 aaaggcgcgg tatccgatga cgaactcaaa gcatttttcg acgcgggcta caaccagcag
     8821 caggcagttg aagtcgtgat gggcgtagcc ttggcaactt tgtgcaacta cgtcaacaac
     8881 ctcggacaaa ccgaaatcaa ccccgaattg caggcttacg cctgatacgg caaaccgccc
     8941 gaatatcggg cggtttttca aaaacacccc ctcaaacaaa aacaagccgc ctaagcggca
     9001 gggaagcagc cgcgcatcag gcgcgccccc gatttccccg caaaccttaa ggaacaaaga
     9061 tgaacgccca aaccctgatt gccaacgttg ccgaattcgt caaaaccaag ctcaaaccca
     9121 tagtggacga catcgaccgc aaaggatact accccgaagc atttatgcgc gaactcggcg
     9181 caatcggtgg attcggcgca gtcggtacag aagccgaagg cggcaacggc ttgggtttgg
     9241 caacgcaaat cgccgtattg cgcgaaatcg gcaaagaatg cggcgcaacc tctttcagtg
     9301 cgtggtgtca ggcggcttgc gcgtggtatc tgcaccaaac gcccaaccgg gccgtcaaag
     9361 acaaatacct tgccgacatc ctgcaaggca aagtattggc gggtacgggc atgtccaata
     9421 ccgtcaaaca ccttgccgac atcgaaaaac acaacctcca agccgagcgc gtggagggcg
     9481 gctacacagt caacggcgcg ctgccgtggg tgtccaacat cggcgaagac cacatttggg
     9541 cgaataccgc ccaaatcggc ggcggctacg tcatgttcat cacaggcgga caatgggaag
     9601 gcgtaagcct gcaaaactgc cccgaatttt gcgccctcga aggcacgcgc accttcagcc
     9661 tgaacttcaa agacgtattt atccccgacg aagacatcat cgccgcgccc gaacagtttg
     9721 ccgactacat ccaaagcatc aaagcaggct ttatcctcct gcaaatcggc atcggcgcgg
     9781 gcgtgataga cggcagcctc ggcatcatcc gcctcgccaa cgtcgtcaac gccgaagtca
     9841 accgctatct cgacgacggc tacgacagcc tcaaaacaag gctggacggc gcgtgggcag
     9901 aaaccgaacg gctcgccgga ttggcttgga gcggcacgcc cgacaacctt accaccctca
     9961 agctgcgcga agccgccgcc gtactgacac ttgccgccgc ccaatccgcc gccctgcatt
    10021 ccggcgcaaa aggctacctg atgcggagtc ccgcccaaag gcgcgtccgc gaagcaatgt
    10081 ttgtcgccat cgtaactccc gcgatcaaac acctccgcaa agaaatagcg gcaatcgaag
    10141 ccgcaaaata aacaggcgga aaaaaccgaa acccccatcg cgtcattccc gcgaaagcgg
    10201 gaatccagcc cccaacgcaa caggaatcca tcggaaaaaa cagaaacccc caaaccgtca
    10261 ttcccgcaaa agcgggaatc cagcccccta acgcaacagg aatccatcga aaaaaccgaa
    10321 acctccaaac cgtcattccc acggaagtgg gaatccagta accgaaaaac cacaggaatc
    10381 tatcgaaaaa aaacccaacc cccaaaaaaa ccgggcggat acctcaccat cctcccaaac
    10441 cctgatttat agcggatgaa caaaaatcag ggcaagacgg cgaaaccgca gacagtacaa
    10501 atagtacg
//
LOCUS       DAWFAD010000058        10217 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWFAD010000058 DAWFAD010000000
VERSION     DAWFAD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.058"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(69..143)
                     /locus_tag="WGO49_001115"
     tRNA            complement(69..143)
                     /locus_tag="WGO49_001115"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Glu,seq:ttc)
     gene            complement(161..237)
                     /locus_tag="WGO49_001116"
     tRNA            complement(161..237)
                     /locus_tag="WGO49_001116"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(201..203),aa:Arg,seq:acg)
     gene            complement(347..2020)
                     /gene="recN"
                     /locus_tag="WGO49_001117"
     CDS             complement(347..2020)
                     /gene="recN"
                     /locus_tag="WGO49_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ0306229.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKSVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSREQAET
                     VRQLYQNWANAKKALQEAQEHADAVIIERERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIALLPSSPTSHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2204..2806)
                     /locus_tag="WGO49_001118"
     CDS             complement(2204..2806)
                     /locus_tag="WGO49_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ0306230.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            complement(2907..3848)
                     /gene="ylqF"
                     /locus_tag="WGO49_001119"
     CDS             complement(2907..3848)
                     /gene="ylqF"
                     /locus_tag="WGO49_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ0306231.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTRAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3929..4783)
                     /gene="rapZ"
                     /locus_tag="WGO49_001120"
     CDS             complement(3929..4783)
                     /gene="rapZ"
                     /locus_tag="WGO49_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ0306232.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEEAVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYVVEKLARRLKGRYELLIRHR
                     QAQNLSDR"
     gene            complement(4764..5726)
                     /locus_tag="WGO49_001121"
     CDS             complement(4764..5726)
                     /locus_tag="WGO49_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ0306233.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGNLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5730..6179)
                     /gene="ptsN"
                     /locus_tag="WGO49_001122"
     CDS             complement(5730..6179)
                     /gene="ptsN"
                     /locus_tag="WGO49_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ0306234.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6364..7254)
                     /gene="ubiA"
                     /locus_tag="WGO49_001123"
     CDS             complement(6364..7254)
                     /gene="ubiA"
                     /locus_tag="WGO49_001123"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ0306235.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVGNSVPVEAWILFTANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            complement(7284..7760)
                     /locus_tag="WGO49_001124"
     CDS             complement(7284..7760)
                     /locus_tag="WGO49_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ0306236.1"
                     /translation="MEHLFGKWLPDLPAAVSDGIDLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDRIPVVEARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7775..8422)
                     /gene="bioD"
                     /locus_tag="WGO49_001125"
     CDS             complement(7775..8422)
                     /gene="bioD"
                     /locus_tag="WGO49_001125"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ0306237.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGMLAKQLLQQGKSVITQKPVQTGC
                     QDIAEDIAVHRKIMGIPMQEADKRRLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEIVLVEGAGGLMVPLTEKLLTIDYIQQQAYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLHSLVFNHIHDSRDAHIAQYSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8419..9720)
                     /gene="bioA"
                     /locus_tag="WGO49_001126"
     CDS             complement(8419..9720)
                     /gene="bioA"
                     /locus_tag="WGO49_001126"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ0306238.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLVDGMSSWWCAIHGYNHPVLNQAVETQIKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYRQVRGLTAKQNIATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNPTSRFDDEWDEADLQPVRALFEVHHADIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKGRLKAAWDIRGVKDVRVLGAIGVIELDKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
BASE COUNT         2377 a         2791 c         2593 g         2456 t
ORIGIN      
        1 tatgagaaat gagaaatgag aaatgagaaa tgagaaatga ctccgatata atcggaggca
       61 ttttgaattg gcacgcccac ggggaatcga accccggtta ccgccgtgaa agggcgatgt
      121 cctaaccgct agacgatggg cgcgtattgt attttcttac tggcgcaccc ggagcgattc
      181 gaacgcccga ccctctggtt cgtagccaga tactctatcc aactgagcta cgggtgcagc
      241 acaatcactt gttcaagtga ttaagaaagc gtgataatac ggatttatct gagttctgtc
      301 tattgtttta gacttttatt tttagttatt ttttaactga ttgattttaa aataaactat
      361 ttttcgacgc aagttgcagc aattctgccg catgttgccg cgtcgtatcg gtaatgactt
      421 ctccgcccaa catacgggca acctcttcga cccgttggat ttcatccaat atactgattt
      481 cgctgacggt ttgctctccc tcgctgtgct tgcgcacccg ccagtggttt tcgccgcagg
      541 atgcgacttg cggaagatgg gtaacggcaa gcacctgatg ttttctgccc aacgcacgta
      601 atgccttgcc gaccatttca gccactcccc ctccaatacc ggtatcgacc tcatcaaaaa
      661 tcagggtggg aacttgggta tattggctgg caacaacctg taaggcaagg ctgatacgcg
      721 ccaattcgcc gccggaggca actttattca gcggacgggg cggattgcct ttgttggcgg
      781 caacttgaaa ctgtacttgc tccaagccgt gcgacgtcgg ggaagacggc agtagggcaa
      841 tgtcaaaacg cgcacctttc atggcaaggt gttgcatatg ctcggtcgtt tcgccgctca
      901 aacgctctgc cgcctgatgg cgcatggcag aaaggatgtg ggcagcttcc tgatattcgg
      961 caaaattgtg ggcaacatta tgctcgagcg cgtccaaatc ggcggcagct tgcaggcttt
     1021 gcaggcgttc ttcgatttct gccaacttgg caggcaactc ttcaggctcg atccggtatt
     1081 tccgcgccat ccccatcagc tcgcccatgc gctgttcctg tgccgccaat tcattgggat
     1141 tgatgtcgct gcgacctgcc acatcgcgca tattggcact gatttcgccc aattcggctt
     1201 cgatgcttgc caacatattc aggctctcgg caaagcgcgg ctcgatgttt tgcagattgg
     1261 ccaatagttt ttgacattga tagatatggc gttggatgcc gttgtcgccg tcaatcttgc
     1321 ttccgacttc ttcggcagcc tgcaacagct cggcagaatg ggcaaggctg tcgtggcttt
     1381 ggctgagggc ttcccattcg ccttgtttaa tgtccaactg attcagttcg ttaaactgcc
     1441 attccagacg ctcccgctcg ataatgacgg catcggcgtg ttcctgcgcc tcttggaggg
     1501 cttttttcgc attggcccaa ttttgataaa gctgcctgac ggtttccgcc tgctccctgc
     1561 tacccgcaaa tgcgtccaac aattcgcgct gggcggcttc ctgattaagc gaatgatggg
     1621 cgttttgccc gtggatgtcg ataagctggc taccgacgga tttgagttgc gccaaggtag
     1681 cggcctgatt gttgataaag ctgcggcttt tgcctttggc atcgataatg cggcggatac
     1741 tgagttcttc ttctccgtcg tttaaaagcc cctgttcata caattctgct tttaaaacag
     1801 gcaaatggga aatatcgaac aatgccgaca actgcgcttc ttttgcaccg ctgcggactt
     1861 ggctgtaatc ggctttgtcg cccaacagca gaccaatcgc atccaaagta atggacttgc
     1921 ccgcgccggt ttctccggtc aatacggtaa agccgctttg aaaatccaga ttcagatttt
     1981 caacaatgac aaaatcacgc aaagaaagtg ttagaagcat tgtgaggatt ccttagcaat
     2041 tctaccgaca aaagcaatat caactttaga tatacgaatt ataactaaga tttttttaaa
     2101 ttaaaaataa gttttcctta attttgctta gtacttgttt ttatctgctt atttgttgca
     2161 atgccgtctg aagcaatgtg cgtttcagac ggcatttgga atttcagttg ggcagggtat
     2221 cggacggtac ggcttccggc tcttcttcat ccgtaggcgg catttccatt aaatcgggta
     2281 aaaccagttc gcccagttcg gtcagcggcg gaagttcttc cagaccgtcc aagcgcaaat
     2341 cgctgagaaa agttgccgtt gtcgcccaca atgcgggttt tcccaatgtg tcccgatgtc
     2401 cgatgacttc aatccacccc cgatcctgca aggtctgcat cacgttctgc gacaccgcca
     2461 cgccgcgtat gccctcgatg tcgccgcgcg ttacgggctg ctggtaggcg ataatcgcca
     2521 gtgtttccat cacggcgcgg gagtaacgcg gcgcacgctg ttcttgcagg ctgcccagcc
     2581 gctcgaatgc cgtctgaaca atctgaaaac gccagccctc ttgcgtatgc accagttgca
     2641 acgccctatc ctgccaacgc gttttcaact gcgccaacac atcaatcagt ttgtcctgcg
     2701 acagtggcgg cacacacaat tcgcgcatag atttttcggt cagcggttcg gtttgggtca
     2761 gcagtgcggc ttcaattagt gcgtcgggag aaattttgtc ggtcatacgg gtatccgtgc
     2821 tgaaacggca tgggtttgga tatgccgtct gaaatcggtt ggagtagaga gaagctgcct
     2881 gaaaaatatt tttcagacgg cattctttat gcttccgaag gcttctgccc tttacgctct
     2941 gcttttctgg cttcgcgtat ggctttgagt tcggcttctt tctgacgggc ttttttgagc
     3001 caagtttccc attggttcgg cgtttcgagg gtgattctgc cgattttgcc ttcacggaag
     3061 tcggtgagga tgttttcggc ggctttttgg tagttgatcc gtccgccgct gaggactgcg
     3121 ccgcgttttt tggctatcca ttcgagccaa acgttttcgt cccagtggct gctggggtct
     3181 ttgtcggctt ggtagcgttc ttgcaacata gggaggtagt ggcggcggag gtagtctaaa
     3241 agttcgaggg cgacttcttc ttcgtccaac gcgttgcgtc cgactgcgcc gccggcggca
     3301 aggttgtagc cgccttcttc gacgatgatt ttcggccaca gcattccggg ggtgtcgtag
     3361 agccagaagt cgtcggcaag gaaaaggcgt tgttcggctt tggtgatgcc gggttcgttg
     3421 ccggttttgg cggatttttt gcctatcatg ccgttgatga gggtggattt gccgacgttg
     3481 ggaatgccgc aaatcaagac gcgcaagggt ttgtctatgc cttgccggtg gggaatcatc
     3541 gcgcggcagg cttgggtgat tttgccgtgt gcgccggttt cggaggaatc gagggcaatg
     3601 gcgcgcgtgt cggggcggct gttgtagtgg tcaagccaga cctttgtgcg ttcggggtcg
     3661 gcaagatctt gtttgtttag gattttaagt ttgggtttgc ctttggaaag ctgggcaagc
     3721 agggggtttt cgctggaggc gggcatacgc gcgtccagca tttcaatcac catatcaacg
     3781 ctttttgcac gctcggcgat ggcttttttc gccttgttca tatggccggg aaaccattgg
     3841 attgccatgt ctgttctttc ttttcaatat ttgaaatgcc gtctgaaacg gaggacgggg
     3901 tttcagacgg cataatggtt tgacggaatt agcggtctga caggttttgc gcctgtctgt
     3961 gccgtatcag caattcataa cgccctttca accttcgggc gagtttttcg acaacataga
     4021 cggaacggtg ctgcccgccg gtacagccga tggcgacggt aacgtagctc ctgctttcat
     4081 cctccaaacg cggcagccaa tgcgtaacaa acctttcgat gtcgtcaacc atttcctgca
     4141 caagcggctg tccgtccaaa taatcccaaa cgggcttgtc cataccggtg taaggcctca
     4201 actcgggatc gtaatacggg ttgggcagac tgcgcatatc gaacataaaa tccgcgttgt
     4261 ttggcacacc gtatttgaac ccgaaggact ccaaaatcac cagcagcccg gtacgttcga
     4321 ccttcagcca ctgccggact gcatggcgga gctgttgggc attcatcttg gaagtgtcga
     4381 tacaataggc gatttcttta agcgggaaca gccattcccg ttctttcttt aagctttcca
     4441 acaaggtcat atcctgatta ctcagagggt ggcctcgcct ggtttcggaa aaccggcgga
     4501 ccagcacggc ctcttccgcc tcgacaaaca aaacttcaac cctgtgcccc agtccgcgca
     4561 gagaggcaat ctgttcccgc gcctgtccga tgtctatgcc ggaacgcaca tcgacgctga
     4621 ccgccaattc ggtttcgtcc gcacgctcga tatgatacga caccagcgcg ggcaacattt
     4681 ccaagggcag gttgtccacg cagaaataac ccgaatcttc catttggcgc agtgcgacgg
     4741 atttgcccga accggacaac ccgctaatca ggacgatttt catgattctg ttcgttttct
     4801 ttaagctgcg tctgatggcg ttccaaaaat tcgcgcgtac tatccttacc gcgcaactga
     4861 agaatataat tgcgcaccgc cgcttcaacc aatacggcaa ggttgcgtcc gaccgcgaca
     4921 ggcagcgtta ccgaacggac gttgacgttg aggatggatt cggtttcggt acggatactc
     4981 aaacggtcga gctgcttcat atactcgtcg tccgcctcga ccaaattgat aatgagttgc
     5041 aggatttttt tggggcggat ggaagtttcg ccgaaaatat ggcggatatt gagtatcccc
     5101 aagccgcgca cttccaaaaa atcgcgcagc ataggcgaac accgcccttc cagcgtttcc
     5161 gggccgatgc ggaacagctc gaccgcatcg tcggcaatca ggctgtggcc gcgcgaaatc
     5221 agctccaacg ccaattcgct cttacccaaa cccgaatgtc cggtaatcag cacgccgatt
     5281 tcaaacacat cgagaaatac gccgtgtttg acggacgatg ccgccagggt gcgttgcagg
     5341 taaatccgca acacgtccat cagatagggg ctttcgagtt tggaagtcag cagtggaata
     5401 tcgtttttgt ggcagtagtc gcgcagtccc ggggaaaccg gcaagccgtt tgccacaatg
     5461 accaaagaca tagaaatgtc gaacaggttt ccgaactgat aacccgtttc ccccgattcg
     5521 aggcggttca gatattccga ctccgccaaa ccgaccactt ggatttggtt ggggtggatg
     5581 aaattcaggt gtccgaccaa agcgaggacg ggcttgtccg cctctacgcc gatacggttg
     5641 tccgcacccg aattgccggc ggcccaggcg agttgcagtt tgtattggtt gtcgtcaaac
     5701 agtcggcgga cagagatact gggcatattt tattcttcgg tcaggatggc gcgggcttct
     5761 tccgcagaag aaaccgtcat cagcgattcg cggatgcttt tttgggaaaa cttgccggcc
     5821 agtttggaca atacctccaa atgctcaccc gtcgcatttt ccggaaccag caagataaaa
     5881 accagggaaa ccggtttgcc gtccggtgcg tcaaatccga cgggttcgcg cgtgcggatg
     5941 aacgcgcccg tcgcctgctt cacgccggca tgacggccgt gcgggatggc aacgccctgc
     6001 cccaaaccgg tcgaaccgag tttttcacgg gcaaaaagac attcgaaaac atcagcatgg
     6061 gacaatgagg attcgcgttc caaaagcagg cccgcttcct caaacagcct ttttttactg
     6121 cccacctcca tatccaaaac aatatgggac aaaggcaaaa tttcgccgat aaggctcata
     6181 agcttctctt ttcagacatc gcaaaacaga aagattgtac cgactgccgg ggcaaatctc
     6241 aatcccgcat acggtacggg ctgacataac acagcgtttt aaaaacatat tttaacgctt
     6301 ttcggcacag atagaaatgc cgtccaaagc agtttacggc tcttcagacg gcattgccct
     6361 gccttatttc gcgaaaaacg tatgggcaaa aatggcggta aaccacaccc aaccaattct
     6421 gttgtttgcc aaaaacgttt caaaacagat ttgccggacg cggcttttga tggcggcata
     6481 ttggcggtat tgcagcagca ggacgatggg gattgccgtc caatatgccc atgccgcacc
     6541 gataaccgcc cccaatactg ccatcagcag ggtaaagccg ccatgacaca gcataacggc
     6601 ggcgatgtcg taacgcccga acgtgacggc ggaggttttg atgccgattt tcaaatcgtc
     6661 ctctttgtcc gccatggcgt aaacagtgtc gtaagccagt gtccataaaa cattggcggt
     6721 aaaaagtatc catgcttcaa cggggacgct gttgcccact gcggcaaacg ccatcgggat
     6781 accgaaggaa aaggcaagcc cgagatagag ttggggaatc ggaaaaaaac gtttggtaaa
     6841 cgggtaagtc agcgcaagaa acagggcggg caggctcatc agccaagtca gatgattcag
     6901 cggaatcagg cacaatgcgg caagcaggca caaaaatgcc gtcagcagca gggcttcttt
     6961 tttcttgacc ctgccctgtg cgaacggacg gtttttggtg cgctcgacag caccgtcaaa
     7021 atcgcggtcg gcaaaatcgt tgatgacgca accggcactg cgcattaaaa acgtaccgat
     7081 tgtaaacgcc gccaataccg ccaaatcggg aatgccgtct gaagccagcc acaatgccca
     7141 gtaggtcggc cacagtaaaa gcagcgtccc aatgggcttg tccgcccgca tcaggcgcag
     7201 gtacacatcc aaacgatcgg acagtcgtaa aaataaaggg gatttaggat tcatattgcc
     7261 gcacagcttg aaaaaacggt attttatccg ataaagcgtt tcagttcggg cagaaaatac
     7321 tcggtcagca gcatttcctc gccgtgacgg gaaaaccgag aacgccgcgc ggcaaagtac
     7381 cgtccgcatc cttcgccgga aacggcaaac tcaaacgccg aacgcgcccc ttccaaatcg
     7441 gcttgaaaca gacgctcgcc caaagggcgc gtgccgcagt ccaaaatgtt ttgccaaaac
     7501 gccgaaccta tccggcattc gctccttgcc tcaacaacag ggatacggtc cagcttcaac
     7561 aaaacttcgc gcaccagcct ccctccgcat tccgtctcca attcgcccag tttcagcagt
     7621 tccaccgaaa atgtatgcgg caaggcgcgc aatgcggcgg tcagcgaccg ggctttcaat
     7681 aaccggctca tcggcaggtc gatgccgtct gaaacggcgg cgggcaagtc gggcagccat
     7741 ttcccaaata ggtgttccat atttttccca atctttatac cgcgtctgtt tttgccaact
     7801 ccatccattc cgcttcggaa aaatcggctt tcagacggca tttcaagtag ctcaggctgt
     7861 attgggcgat gtgtgcgtcg cggctgtcat gaatatggtt gaatacgagg ctgtgcaagc
     7921 gaatgccgta ttgtttgagc gcggcgaaac tgagcagggt gtgattaatg ctgccgagcc
     7981 gaccgctggt aacgaggatg acgggataag cctgttgctg aatataatca atggttaaca
     8041 gcttttccgt cagcggaacc atcaaaccgc ccgcgccttc gaccaagact atttcgtatt
     8101 gtgccgccaa ttcttgcgta gcggtacgga ttttgtccaa gtccaaagcc ctgccgtcca
     8161 gacgggcggc gagatggggc gaggcgggat agctgaagat ttcaggcata gtcagccgcc
     8221 gtttgtcggc ttcctgcatg ggtatgccca tgattttgcg gtgaacggcg atgtcttcgg
     8281 cgatgtcttg gcaaccggtt tgcacgggct tttgcgtaat cacgcttttg ccctgctgca
     8341 acaattgttt tgccaacata ccggtggcga cagttttgcc gatgtccgtg tctatgccgc
     8401 tgacgaagta aacgcctttc atttgctgtg ttccttcaag atttgcacgg ttttgtcggc
     8461 aagtttggtc aaaacgccgt ctgaaataat atacggcggc atcagatata ccagcctgcc
     8521 gaacgggcgc acccaaatgc cctgcgccac gcaatctgct tgaaaacgcg ccatatccac
     8581 gcctttatcc agctcgatca cgccgatggc acccaaaacg cgtacgtctt tcacgccgcg
     8641 aatgtcccac gcggctttca gacggccttt taagatgctt tcaatgcggc ggatatttgc
     8701 ctgccagtct tgagacaaaa gcagtttgac cgaagcgcag gcaacggcac acgccagcgg
     8761 gtttgccata aacgtcgggc cgtgcataaa cacgcccgct tcgccgcgcg aaatcgtttc
     8821 ggtaactttt tgcgaagtga ttgctgccgc cagcgtcata tagccgccgc tcaaaccctt
     8881 gccgatgcac ataatatccg gcacgacctc cgcgtgttcg caggcaaaca tcttgcccgt
     8941 gcgcccgaat ccggtggcga tttcatcaaa aatcaaaacg atatcaaact cgtcgcacaa
     9001 atcgcgcaat ccgcgaagat actgcggatg ataaaaatac atgccgcccg cgccctgcac
     9061 gaccggctct aaaataaagg cggcaatatc cgcatgatgc acttcaaaca aggcgcggac
     9121 aggctgcaaa tccgcctcgt cccattcatc gtcgaaacgg cttgtcgggt tatcgacaaa
     9181 ataacgctgc ggcaacgcgc tgccgaaaat atggtgcatc cccgtttccg gatcgcagac
     9241 ggacatcgcg ttccaagtat cgccgtgata cccgcgccgc accgtcgcaa tattctgctt
     9301 cgccgtcaac ccccgcacct gccggtattg caccgccatt ttcaacgcaa cttccaccga
     9361 aaccgaacct gaatccgcat aaaaaatacg gtccaacccc tgcggcaaaa tcccaaccaa
     9421 caatttaccc agctccaccg caggctcatg cgtcaaaccg ccgaacatca catgcgacat
     9481 ttgtttaatc tgcgtctcaa ccgcctgatt caaaacagga tgattgtaac cgtgtatcgc
     9541 acaccaccag gacgacatcc cgtcaaccag ccgcgtgccg tccgccaatt cgataaacac
     9601 cccttctgca cgtttgacag gataaacggg aagcggatcg gtcatggaag tatagggatg
     9661 gagcagatgg gtacggtcga aatcaagtaa tgatgatgtg tgttgatgtt cagacggcat
     9721 aagtttctct cttttcttat tgctgcattc aaacgcaaac gcgtattcta ctccgacaga
     9781 ccgtttccca cacctctcca tccgtttcgg gcgcaaaacc gcgaaacaaa tcgtccgcag
     9841 tataagcgca caccgtttcg cattccccaa gcccgattgg aatcagacga cccaacgccc
     9901 aatattgttt cacacagcct ccgcccaacg cttcaatcag tcatagacaa aacggcaatc
     9961 catacgatac agatcatagc aacagccatc gcaacagcgt tagcaaaatc aggggactcc
    10021 gacataggcg catagcatct accgatgcac agctcctcat tcggctctat gaataccata
    10081 cccatcacaa aatccaccgc caaaaccagg cacggcttct tatacttatg atagatttcc
    10141 accatcctgc cccatatatc ccaaacattc ataccgcata tcccgcaggc aacaaattcc
    10201 gattgaaggt tacagcc
//
LOCUS       DAWFAD010000059        10156 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWFAD010000059 DAWFAD010000000
VERSION     DAWFAD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.059"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(257..1474)
                     /locus_tag="WGO49_001127"
     CDS             complement(257..1474)
                     /locus_tag="WGO49_001127"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ0306239.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVEAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLAAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1676..2404)
                     /gene="rph"
                     /locus_tag="WGO49_001128"
     CDS             complement(1676..2404)
                     /gene="rph"
                     /locus_tag="WGO49_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ0306240.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2486..2950)
                     /locus_tag="WGO49_001129"
     CDS             complement(2486..2950)
                     /locus_tag="WGO49_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ0306241.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3167..3943
                     /locus_tag="WGO49_001130"
     CDS             3167..3943
                     /locus_tag="WGO49_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ0306242.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPGCQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            4188..5042
                     /locus_tag="WGO49_001131"
     CDS             4188..5042
                     /locus_tag="WGO49_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ0306243.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIEAYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTETVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5083..6291
                     /gene="pncB"
                     /locus_tag="WGO49_001132"
     CDS             5083..6291
                     /gene="pncB"
                     /locus_tag="WGO49_001132"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ0306244.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEAELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     AEGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AATQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6383..8101
                     /gene="argS"
                     /locus_tag="WGO49_001133"
     CDS             6383..8101
                     /gene="argS"
                     /locus_tag="WGO49_001133"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ0306245.1"
                     /translation="MNLHQTVEREAAAAFTAAGISGSPVVLQPTKNAEHGNFQINGVM
                     GTAKKAKQNPRELAQKVAEALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGIAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVSEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPESVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGSIKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDANAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            8639..10057
                     /locus_tag="WGO49_001134"
     CDS             8639..10057
                     /locus_tag="WGO49_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0306246.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHGKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLNFKIK"
BASE COUNT         2314 a         2984 c         2571 g         2287 t
ORIGIN      
        1 cagtacggcg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc
       61 aagtgaatca gttccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt
      121 tgttaatcca ctataaattt aatccactat attttttgtt ccaaagtcaa aatatgccgt
      181 ccgaacattc gggcggcaga caaaacggca ctgcccgata aaggcagtgc cgttgtccgt
      241 ttcaaaccgt gaaacatcag cccaaattaa aggctttatg caataccctg gttgccagtt
      301 ccatgtattt ttcatcaatc aatacggaaa ctttgatttc ggaggtggaa atcatttgga
      361 tgttgatacc ctcttcggcg agcgtgcgga agattttggc ggctacaccg acgtgcgaac
      421 gcatacccaa accgactgcg gagactttgc acacggtgtc gtcgccgtcg atagaagccg
      481 cgccgatact gtcttgacgt tccgacagga tttccaaagt ctgcttgtaa tcgccgcgcg
      541 gtacggtaaa ggaaaaatcg gttgtgcctt cgctgccgac attttggata atcatatcga
      601 cttcgatgtt ggcatcggca accgcgccta aaatctgata ggcgacgccg ggtttgtcgg
      661 gtacgccgcg cacgttgatg cgggcttggt ttttgtcgaa tgcgataccg gttacggcag
      721 ctctttccat attgtcgtcc tcttcaaagg taattaaggt gccgttgccg ccgtcttgca
      781 ggctgctcag tacgcgcagg cgcactttgt attttccggc aaattctact gaacggattt
      841 gcaaaacttt cgagccaagg ctggccagct caatcatctc ttcaaacgtt accgtatcca
      901 tgcggcgcgc ttcgggtacg acgcgggggt cggtggtgta aacgccgtcc acgtcggtat
      961 agatttggca ttcgtctgct ttgagggctg cggcgagcgc aacggcggag gtgtcggagc
     1021 cgccgcgtcc gagcgtggaa atgtcgcctt cgctgctgat gccttggaaa ccggcgacga
     1081 tgacgacttt gccggcggca aggtcggcac gcattttttc gtcatcaatg ctttcgatgc
     1141 gggctttggt gtgggcggta tcggttttga gggcgacctg ccagccggtg tagcttttgg
     1201 cttccacgcc gatgtctttc aatgccatcg ccaaaaggcc gatggttact tgttcgccgg
     1261 tggacaaaac cacgtccaat tcgcgcggat cgggatgctc ctgcatttcg tgcgccaacg
     1321 cgaccaggcg gttggtttca ccgctcatgg cggatacgac gacgacgatg tcgtgtcctt
     1381 cggcgcgggc tttggcgaca cgtttggcca cgtttttaat gcgttcgggc gagcctacgg
     1441 atgtgccgcc gtatttatgt acgattaacg ccatgtttcg tgctttcttg tgtgggattg
     1501 gtcgggcagc atggtttgct ggaaaagcgg ctattattac tattttttac atgaagttca
     1561 agaacggact gcgctttccc gcctgccgtt tgacagcggt cagcgaaaaa cctgttcttt
     1621 cagattgttg acaaaatgcc gtctgaacgg ttttcagacg gcatccggac gacaatcagg
     1681 cggcggacaa cgcattttgc tggtgttgca gcagttcgcc tatgcctttt tgcgccagtg
     1741 caaccagttt gcccaattcg tccaaactga acggcgcgtc ttccgccgta ccttggattt
     1801 cgatgatttt tcccgatgcg gtcatgacga tattcacatc actgtcgcaa ccggagtctt
     1861 cgggataatc caaatccaaa agcggcacgc cgttcactac gcctactgac acagcggcaa
     1921 cggcttcgcg gatggggttt tcactcaaaa tgccgtctga aaccagtttg ccgacggcga
     1981 tttgcagcgc gacaaacgca ccggtaatcg aagccgtgcg cgtaccgcca tctgcctgaa
     2041 tcacatcgca gtcaatcaag atttgtcgtt cgccgagttt ttccatatct acgaccgcgc
     2101 gtagcgaacg cccgataagg cgttggattt cttgtgtgcg cccggactgt ttgcccgccg
     2161 aagcttcgcg gcgcatccgg gaagcagttg aggcaggcag catcccgtat tccgccgtta
     2221 cccagccttg gtttttaccg cgcagaaacg gcgggacgtt ttcatctatg gaagcggtac
     2281 aaatcacttt ggtattgccg cattcaataa ggcacgaacc gtccgtatgc ggcaggaaat
     2341 gaggggtgat tttgatatcg cgcaggctgt ccgcggcgcg cgagatgcgg atgtaatcag
     2401 gcatactgcc ctcccgttaa aaacagataa attaaaaagc cttaaatatg aaaaatcaca
     2461 tttaaggcct tcaaactgaa aatttctacg cctcttcggc tttgctgcgg ataatcaaaa
     2521 gcggcaggtg gctttggcgc attaccgttt cggcaaaact gcccattaaa aggtgcatca
     2581 gtccggtacg tccgtgcgta cccaacacca gcaggtcggc accgttttca tcggcataat
     2641 caaccaaatc ctgcgccatt tcacgcgcac ccttattggc aaccagcagg tgtttgacgg
     2701 tattttccac acccagttcc cgggcggtgc gctcggcggc atccaaaact tcgttgcctt
     2761 gcgcgacggc ggcggcttcg tagctttcgt gttgcaaaaa ttcgggggcg agcgccatat
     2821 attcggcagg attggcaacg tgtaccaaag tcagacgcgc accgttgacc ccggcaagct
     2881 cggcggcatg tttcagggca ttgatggacg tttcactgcc gtcaacggca acaaccagat
     2941 gtttgtacat atcgtattct ccttttgcac cgcctcgcgg tgccctcttg tcggatgggc
     3001 gcagggacag tttgcgccgt ttcattatag acccgctgtc gggctttata caacagccga
     3061 acagcccgac cgctttccag tataatatgc cgcttccgtg cagtcaggca ttttttgccg
     3121 gctttcgttc actttttgat ttgacgcaat cttgcaggat tcgaccatgt ccgacaacgc
     3181 tttgacctct tcgcgacgct tcggcggcat cgccagactc tacggagact ctgccttggc
     3241 gcacttttca caggcacacg tctgcgtagt cggcgtgggc ggtgtcggct cgtgggcggt
     3301 cgaggctttg gcgcggacgg gcatcggacg tttgactttg attgatttgg acaacgttgc
     3361 cgaatcgaat gtcaaccgcc agctgcacgc cctgaccggc gacttcggca aagcaaaagt
     3421 taccgccttg cgcgaacgcg tcgcccaaat caacccagga tgccaagtgt ttgaaattga
     3481 agatttcgtt accgaagaca atttgccgga atacttcgga aaaggttttg atttcgtcat
     3541 cgacgcgatc gaccaagtgc gcgtcaaagc agcaatggcg gcttattttg tggaacgcaa
     3601 acaaccgttt gtcctcagcg gcggcgcggg cggacaaaaa aatccggcgt taatccaaac
     3661 cgccgatttg agccgcgtaa cccacgaccc gctgcttgcc aacctgcgct acaccttgcg
     3721 gaaacgctac ggattcagcc gcgatacgaa agcaaatatg cgcgtgcctt gcgtgtattc
     3781 gaccgaaaat atcgtgccgc cgcagtctag ggaggcttgt tcggcagatg ccgctccgca
     3841 aggcttgtcg tgcgccggct acggtgcaag catgctcgtt accgcttcgt tcgggctata
     3901 ttgcgcacag gcggcggtgg aacacatcgc agacaaaaaa taagcaatgc cgtctgaaac
     3961 aggattcaga cggcatttga acaaacgcgg caatcaagct gtcggttcag aaaaaccaac
     4021 atcggcacgc ctaaaaccgt attcacaacc tgctcctttt caaaacattt gcatttaaaa
     4081 gccgttataa tgccgtctga acatctgccc gaccacatta tgcgtgaatg tcggcagatt
     4141 gttttctttt gtaaacttat attaaaatcc acttaccgat tcacgccatg ccgcccatcc
     4201 ctgccccatc cgcaccatcc gagcacactg tcgcatgggt attcggccaa cccgttaccg
     4261 atttgccgca ggatttgttt atcccgcccg acgcgctgaa agtcgtattg ggcagttttc
     4321 aaggcccttt ggatttgctg ctctacctta tccgcaagca gaacatcgat gttctcgata
     4381 ttccgatggt gaagattacc gagcagtatc tgcactacat cgcccaaata gaagcctatc
     4441 agtttgattt ggcggcggaa tacctgctta tggcggccat gctgattgaa atcaaatcgc
     4501 gcctgctgct gccgcgtacc gaaaccgtcg aagacgaaga agccgacccg cgtgccgagt
     4561 tggtgcgccg cctgctggct tacgaacaga tgaagctggc ggcgcagggt ttggacgcgc
     4621 tgccccgagc cggacgggat ttcgcgtggg cttacctgcc gctggaaatt gccgtcgaag
     4681 ccaagctgcc cgaagtctat attaccgact tgacgcaagc gtggctgggt attttgtctc
     4741 gggcaaaaca cacgcgcagc cacgaagtaa tcaaagaaac catctccgtg cgcgcgcaaa
     4801 tgacggcaat cctgcgccgt ttgaacggac acgggatatg caggtttcac gacctgttca
     4861 atcccgaaca gggcgcggct tacgtggtcg tcaacttcat cgcactgttg gagcttgcca
     4921 aagaaggttt ggtcggaatc gtacaggaag tcggtttcgg agaaatccga atcagcctca
     4981 atcatgaggg ggcacattca gacggcattt ccggcacacg gggcgggcgc gatgtgttct
     5041 aatacgcccc aagccgccac caaaaatcgg gagacacgcc atatgaccgg catcatacat
     5101 tcgctgcttg acaccgacct ctacaaattc actatgctgc aagtggttct gcaccagttt
     5161 ccgcagacgc acagccttta cgaattccgc tgccgcaacg cctcgaccgt ctatccgctt
     5221 gccgacatca aggaagactt ggaagccgaa ctcgacgcgc tctgccggct gcgcttcacc
     5281 cacgacgaac tcggctatct gcgctccctg cgtttcatca aaagcgactt tgtcgattat
     5341 ctcgaactct tccagctcca acgccgcttt gtcgaagtcg gcaccgatgc cgaaggccgt
     5401 ctgaacatcc gtatcgaagg ccccatgatt caagcgatgt tctttgaaat cttcatcctt
     5461 gccattgtca acgaacttta cttccgccgc ttggaaaccc ctgcagtcat agaagaaggt
     5521 gaacgtcggc ttcaagccaa agccgcgcgt ctgaaagaaa tagccgccac acagaatccg
     5581 gacgaaccgc ccttcctgat ttccgacttc ggcacgcgcc gccgctacaa gctcgcgtgg
     5641 caggaacacg tcatccgcac cctgctcgaa gccgccccaa gcatcgtgcg cggcaccagc
     5701 aatgtctttc tcgccaaaaa actcggcatt acccccatcg gcaccatggc gcacgaattc
     5761 ctccaagcct tccaggccct cgacgtacgc ctgcggaatt tccaaaaggc cgcgctcgaa
     5821 agctgggtgc acgaataccg gggcgatttg ggcgttgccc tgaccgacgt ggtcggtatg
     5881 gatgccttcc tgcgcgattt cgacctctat ttcgccaaac ttttcgacgg gctgcgccac
     5941 gacagcggcg acccttacgt ttggggcgac aaagcctacg cccactatca aaagctcaaa
     6001 atcgacagcc gcaccaaaat gctgaccttc tccgacgggc tggacatcga acgctcttgg
     6061 gcattacacc aatatttcaa agaccgcttc aaaaccggct tcggcatcgg caccaacctg
     6121 accaacgata tggggcatac gcctttgaat atcgtgttga aactggtcga atgcaacggg
     6181 cagtccgtcg ccaagctgtc cgactctccg ggcaaaacca tgaccaacaa cagcaccttc
     6241 ctcgcctacc tgcgccaagt gttcgacgta cccgaacccg aaacgccgta aaccggcaga
     6301 aaaagcgcac aattcctgtt tctgccgcat aaaatctttt aaaataccgc ctgatttgaa
     6361 tttaaccgaa agaccgaact tcatgaacct acatcaaacc gtcgaacgcg aagccgccgc
     6421 cgcctttacc gccgcaggca tctccggcag ccccgttgtc ttgcagccga ccaaaaacgc
     6481 cgaacacggc aatttccaaa tcaacggcgt gatgggtacg gcaaaaaaag ccaaacaaaa
     6541 tccgcgcgaa ttggcgcaaa aggttgccga agcattggcg ggcaacgccg tcatcgaaag
     6601 cgcggaagtc gccggtcccg gttttatcaa cctgcgcctg cgccccgaat ttctcgcgca
     6661 aaacattcag acggccttga acgacgcgcg tttcggcata gcgaaaaccg acaaaccgca
     6721 aaccgtcgtt atcgactatt cttcgcccaa tctggcgaag gaaatgcatg tcggtcacct
     6781 gcgttccagc atcatcggcg acagcatttc gcgcgtgtca gaatttatgg gcaataccgt
     6841 tatccgtcaa aaccacgtcg gcgactgggg tacgcagttt ggcatgttgg tcgcttattt
     6901 ggtcgagcag caaaaagaca atgccgcatt cgagctggcg gatttggagc agttttaccg
     6961 cgccgccaaa gtgcgctttg acgaagaccc cgcctttgcc gacaccgcgc gcgaatacgt
     7021 tgtgaagctg caaggcggcg atgaaaccgt tttggcattg tggaaacagt ttgtcgatat
     7081 ttcgctctcg cacgcccaag ccgtttacga cacgctgggc ttgaagctgc gccccgaaga
     7141 cgtggcgggc gaatcgaaat acaacgacga tttgcagccc gtagtcgatg atttggttca
     7201 aaaaggtctg gcagtcgaag acgacggcgc aaaagtcgtg ttcttggacg agtttaaaaa
     7261 caaagagggc gaacctgccg catttatcgt gcaaaaacaa ggcggcggct tcctttatgc
     7321 ttccaccgac ttggcgtgtt tacgctaccg tgtcggcacg cttcatgccg accgcctgct
     7381 gtacgtcgtc gaccaccgcc aagccctgca ctttgagcaa ctctttacca cttcacgcaa
     7441 agccggctat ctgccggaaa gcgtcggcgc ggcatttgtc ggcttcggca cgatgatggg
     7501 caaagacggc aaaccgttca aaacacgcag cggcgacacc gtgaaactgg tcgatctgct
     7561 gaccgaagcc gtcgaacgcg ccaccgcttt ggtgaaagaa aaaaatcccg aattgggtgc
     7621 ggacgaagcc gctaaaatcg gcaaaaccgt cggcatcggc tcaatcaaat acgccgattt
     7681 gagcaaaaac cgcaccagcg attatgtgtt cgactgggac gccatgctct cgtttgaagg
     7741 caacaccgcc ccctatctgc aatacgccta cacccgcgtg caaagcgtgt tccgcaaagc
     7801 aggcgaatgg gatgcaaatg cgccaaccgt tttgaccgaa ccgctggaaa aacagcttgc
     7861 cgccgagctg ctgaaatttg aagacgtact gcaaagcgtg gcggacacgg cgtatccgca
     7921 ctacctcgcc gcctacctct atcaaattgc gaccctgttc agccgcttct acgaagcctg
     7981 tccgatactc aaagccgaag gcgcaagccg caacagccgc ctgcaactgg cgaagctcac
     8041 cggcgacacg ctgaaacaag gcttggattt gctgggcatc gatgtgttgg acgtaatgta
     8101 aaaccgcacc gcccgattgc ggacaacagc ctcgccatcc ttatccgaat ctgaaaaaag
     8161 cggcgcgata caccgtatcc gccgcccctc ccaaaatgcg aaacaaacaa acgccaagca
     8221 agcaagcaag caagcaagca agcaagcaaa aaattataac ccccttcctg ccgacgcacg
     8281 cactttccgc gcggcgcatt ccccttttcc cgcccctcaa atccgccttt tcttcaggca
     8341 gggtttcagc ccgcctcttt tccctgtttt cctttccccg acacgcgtgc gcgccccctg
     8401 ccgcactgtg ctgcactttc gcgcccggac ggcatcgttc cgccatccgg ttctctgttt
     8461 taaatacccc tgtttcagaa agaaatgcag atgtttcaac acacaggacg acacataaag
     8521 caccgcccta tgtgttgccc tgatttggaa ggggttacgc ctcccaaata aagtctgatc
     8581 ctgccgcccc gaaggacaga tgtccgagtg gcgaagtttc aaccgaaaag gaaatacgat
     8641 gaatattcac accctgctct ccaaacaatg gacgctgccg ccattcctgc cgaaacggct
     8701 gctgctgtcc ctgctgatac tgcttgcccc caatgcggtg ttttgggttt tggcactgct
     8761 gaccgccacc gcccgcccga ttgtcaattt ggactatctt cccgccgcgc tgctgatcgc
     8821 cctgccttgg cgtttcgtca aaattgccgg cgtattggcg ttttggctgg cggttttgtt
     8881 tgacgggctg atgatggtga tccaactctt cccttttatg gatctcatcg gcgccatcaa
     8941 cctcgtcccc ttcatcctga ccgcccccgc cccttatcag ataatgaccg ggctgttgct
     9001 gctgtatatg ctggcgatgc cgtttgtgtt gcagaaagcc gccgccaaaa ccgacttccg
     9061 gcacattgcc gtctgcgccg ccgttgtggc ggcagccggc tatttcaccg gccatttgag
     9121 ttactacgac cggggtcgga tggccaatat cttcggcgca aacaacttct actacgccaa
     9181 aagtcaggcg atgctctaca ccgtcagcca gaatgccgac tttattaccg ccggcctggt
     9241 cgatcccgtc ttcctcccct tgggcaatca acagcgtgcc gccacgcatc tgaacgagcc
     9301 gaaatctcaa aaaatcctct ttatcgtcgc cgaatcttgg gggctgccgg ccaatcccga
     9361 acttcaaaac gccacttttg ccaaactgct ggcgcaaaaa gaccgttttt cggtttggga
     9421 aagcggcagt tttcccttta tcggcgcgac ggtcgaaggc gaaatgcgcg aactgtgcgc
     9481 ctacggcggt ttgcgcgggt tcgcactgcg ccgcgcgccc gacgaaaaat ttgcccgctg
     9541 cctccccaac cgtttgaaac aagaaggtta cgccaccttt gcgatgcacg gcgcgggcag
     9601 ttcgctttac gaccgcttca gctggtatcc gagggcgggc tttcaagaaa tcaaaaccgc
     9661 cgaaaacctg atcggtaaaa aaacctgtgc cattttcggc ggcgtgtgcg acagcgagct
     9721 gttcggcgaa gtgtcggcat ttttcaaaaa acacggcaaa ggactgtttt actggatgac
     9781 gctgaccagc cacgccgact atcccgaatc cgacattttc aaccacaggc tcaaatgcac
     9841 cgaatacggc ctgcccgccg aaaccgacct ctgccgcaat ttcagcctgc acacccaatt
     9901 cttcgaccaa ctggcggatt taatccaacg ccccgaaatg aaaggcgtgg aagtcatcat
     9961 cgtcggcgac catccgccgc ccgtcggtaa cctcaatgaa accttccgct acctcaaaca
    10021 ggggcacgtc gcctggctga acttcaaaat caaataacaa caatgccgtc tgaacgcacc
    10081 aacagccttc agacggcatt ttgcagacag accgaccctt caagcccact tttttcatca
    10141 tctccgataa attgct
//
LOCUS       DAWFAD010000060        10099 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWFAD010000060 DAWFAD010000000
VERSION     DAWFAD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.060"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(129..464)
                     /locus_tag="WGO49_001135"
     CDS             complement(129..464)
                     /locus_tag="WGO49_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0306247.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            593..1498
                     /gene="mtrA"
                     /locus_tag="WGO49_001136"
     CDS             593..1498
                     /gene="mtrA"
                     /locus_tag="WGO49_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ0306248.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKKPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1560..2048)
                     /locus_tag="WGO49_001137"
     CDS             complement(1560..2048)
                     /locus_tag="WGO49_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0306249.1"
                     /translation="MKKYLIPLSIVAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2195..3097
                     /locus_tag="WGO49_001138"
     CDS             2195..3097
                     /locus_tag="WGO49_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0306250.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPCWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3138..4268)
                     /locus_tag="WGO49_001139"
     CDS             complement(3138..4268)
                     /locus_tag="WGO49_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0306251.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPPAEKPAPAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4473..7097
                     /gene="alaS"
                     /locus_tag="WGO49_001140"
     CDS             4473..7097
                     /gene="alaS"
                     /locus_tag="WGO49_001140"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ0306252.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQVQIEEALKNEESRFAQTLETGMALLENALAKGSKKLDGEI
                     IFKLYDTYGFPYDLTADICRERNIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAVLANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKALTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPAVLDSVKDWVGAKLV"
     gene            complement(7186..8208)
                     /locus_tag="WGO49_001141"
     CDS             complement(7186..8208)
                     /locus_tag="WGO49_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0306253.1"
                     /translation="MLTKKEFEKFLDEVCTVLRQEAKNAPFKSQDIFENRVRALCIAK
                     IPAYADIVINPEPVPQIFPDVPIGEYGVEVKFTLKDTWRSVANSIQEKNKADGVQHIY
                     VVFGKMGGIPDVKWQLYEDSVIHVRTSHVPRFEVEIESDRPSLFEGFGISYAEFADLD
                     MHEKMEYIRKYARNRLKEGERLWWIEDIDAVDSHDLPLEVRLYTSLPQEEKIRLRAEA
                     ALVSPRIVCSGRAKHKYDNAVLYLITYRGVLCHQARDLFSAGSVANSAEDYRGGNYVE
                     KSLKLIEHEIEKAALEMDDALIVEYWGESVPTEKRLEYWIKMLDDLADGWIPSESLFN
                     GRYKAK"
     gene            complement(8262..9413)
                     /locus_tag="WGO49_001142"
     CDS             complement(8262..9413)
                     /locus_tag="WGO49_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0306254.1"
                     /translation="MTEFARKNWNKLAGDAIKAKMHEHGIGFIQLSELFLEQGESETA
                     NAICNKVNRGTFSHAFYLKCMELMTKQEKQEDREKKYKIISLFSGCGGMDLGFLGGFE
                     FLNRTYDKHDFDIIWANEIDEHAVRTYRHNLGNHIVHGDIWQHLDEIPTSADIIIGGF
                     PCQDISINGKGAGISGSRSGLYKAMLSAVERVRPKIFVAENVKGLLMKHHRKDLETVL
                     SDFRNLGYQVDYYLLNAADYGVPQTRERVIIVGRLPEIPEIQIEKTNTSNSYVTARQA
                     IGDLVDKVEDEIANHIWSRAEKSPDQGSRKLDAERAGYTIRAECHGNIQWHYELPRRI
                     SMREAARIQSFPDNFIFQAKLRATERQIGNAVPPVLGWHIAAAVSKALK"
BASE COUNT         2506 a         2673 c         2391 g         2529 t
ORIGIN      
        1 cttacccctt gtttctcaaa tgccgtctga aaaaacggct gcatatgttg tctacaaaat
       61 atcgggaagc ataacaggca aaatgccgtc tgaaatattt tcagacggca tttcttatcc
      121 gaaacggatt atttttgcgt ttcaaccgct tccaatgcac gcagggcata agtgtaagcg
      181 gcaccggcat tcagggcgat ggcggttgcc aatgcgcctg cgatttcgct gtcggtcgca
      241 ccggctttgg cggcagcggc ggcgtgaacg ctgatgcagc tctcgcaacg agtggtaatg
      301 gcaacggcga tggcaatcag ttcgcgcgtt ttggcatcaa gtgcttcggc ggcggcggcc
      361 tgttccaatg cgccgtaggc ctgcagcatt ttaggatgcg ccttacccag ctcgccgaac
      421 gattttttaa ccaatgcggt atgttctttc caatctttaa acattttctt ttcctttctc
      481 ttgcgtttaa ccctgatacg cgcttgcgta tctgttttcg atgtgcgtat tattgcaatt
      541 attcagttgt gtttctcgtt taatcatctc attttatggt tcaaaaagat ttatggacat
      601 tctggacaaa ctggtcgatt tcgcccaatt gacgggcagt gtggatgtgc agtgcctttt
      661 gggcggacaa tggtcggtac ggcatgaaac cttgcaacgc gaaggattgg tacacattgt
      721 tacatcgggc agcggctatc tctgcatcga cggcgaaact tccccgcgtc cggtcagtac
      781 aggggatatt gtatttttcc cgcgcggctt gggtcatgtg ttgagccacg acggaaaatg
      841 cggagaaagt ttacaaccgg atatgcggca gcacggtgcg tttacggtca agcagtgcgg
      901 caacggacag gatatgagcc tgttttgcgc ccgtttccgc tacgacaccc acgccgattt
      961 gatgaacggg ctgcctgaaa ccgtttttct gaacattgcc catccgagtt tacagtatgt
     1021 ggtttcaatg ctgcaactgg aaagcaaaaa acctttgacg gggacggttt ccgtggtcaa
     1081 cgcattgtcg tctgtcctgc tggtgcttat cctgcgcgcc tatctcgaac aggataagga
     1141 tgtcgaactc tcgggcgtat tgaaaggttg gcaggacaaa cgtttgggac atttaatcca
     1201 aaaggtgata gacaaaccgg aagacgaatg gaatgtcgac aaaatggtgg cggctgccaa
     1261 tatgtcgcgc gcgcaactga tgcgccgttt caaaagccgg gtcggactca gcccgcacgc
     1321 ctttgtgaac catatccgcc tgcaaaaagg cgcgttgctg ctgaaaaaaa aaccggattc
     1381 ggttttgtcg gtcgcactgt cggtaggctt tcagtcggaa acgcacttcg gcaaggcgtt
     1441 caaacggcaa tatcacgttt cgccgggtca ataccggaaa gaaggcgggc aaaaataaat
     1501 cggggcttca aacgcaaatg ccgtctgaaa aggctttcat acagcatttg cgtaccgcgt
     1561 catttcaagg gctgcatctt catcacttcc atcaaaaagt tggtaaatgc ggggttgttg
     1621 ggtttgacat ccatattttt ccaacgctgc tgccagccgc gcaaggcatt ctggatatac
     1681 agcttggact gttccgtatt gattgcgccc cgctggctgt ctatcgccga acgcaggtag
     1741 atttcataca tactgtcatc gacggcattg cgtccgacca ggcgttttct gaagttgttc
     1801 agatattgcg ccgcctgaac cttggtcatt ttaccgatac ccacctgata gcccaagcgc
     1861 gtcgcttcat cgctgatttt ggcaacatcc gtccaatgcg aagaggcaag gcggaaacct
     1921 tttgcaggtg cttccgtttt gacggtattg ataggattca cggggatttc cgtcaatgtg
     1981 ggcacataaa tagactggca gccggaaaga actgccacaa tggaaagagg gataaggtat
     2041 tttttcatgc ccccattata atcaagtttg ccttgagaaa acaaattgtt cggcaagaaa
     2101 aataaaattt cggcatcaga agcaggcaaa aacacattcc acaagccttg ccgcaaggtt
     2161 tacaatccga ccgtccttat cgcaacgacc gtttatggat accgcaaaaa aagacatttt
     2221 aggatcgggc tggatgctgg tggcggcggc ctgctttacc attatgaacg tattgattaa
     2281 agaggcatcg gcaaaatttg ccctcggcag cggcgaattg gtcttttggc gcatgctgtt
     2341 ttcaaccgtt gcgctcgggg ctgccgccgt attgcgtcgg gacaccttcc gcacgcccca
     2401 ttggaaaaac cacttaaacc gcagtatggt cgggacgggg gcgatgctgc tgctgtttta
     2461 cgcggtaacg catctgcctt tggccaccgg cgttaccctg agttacacct cgtcgatttt
     2521 tttggcggta ttttccttcc tgattttgaa agaacggatt tccgtttaca cgcaggcggt
     2581 gctgctcctt ggttttgccg gcgtggtatt gctgcttaat ccctcgttcc gcagcggtca
     2641 ggaaacggcg gcactcgccg ggctggcggg cggcgcgatg tccggctggg cgtatttgaa
     2701 agtgcgcgaa ctgtctttgg cgggcgaacc ctgctggcgc gtcgtgtttt acctttccgt
     2761 gacaggtgtg gcgatgtcgt cggtttgggc gacgctgacc ggctggcaca ccctgtcctt
     2821 tccatcggca gtttatctgt catgcatcgg cgtgtccgcg ctgattgccc aactgtcgat
     2881 gacgcgcgcc tacaaagtcg gcgacaaatt cacggttgcc tcgctttcct atatgaccgt
     2941 cgttttttcc gccctgtctg ccgcattttt tctgggcgaa gagcttttct ggcaggaaat
     3001 actcggtatg tgcatcatca tcctcagcgg cattttgagc agcatccgcc ccactgcctt
     3061 caaacagcgg ctgcaatccc tgttccgcca aagataaaaa atgccgtccg aacatccttc
     3121 agacggcata tcgggcttta tttccccgcc ttcacatcct gccactggcg caccataaac
     3181 ttcaatgccg ccggctggat aggcaccatg ataaagctgt ttttcaaatc ctcctcggtt
     3241 gggaaaatcg tattgtcgtt tttaaattcg tcttccatca gctcacgcgc cggcttgctc
     3301 gaaggcgcat aagtaacgaa attgccgttt ttcgccgaca cttccgggtc gaggaagtcg
     3361 ttgatgtatt tgtgcgcgtt ggcgacgttt ttcgcatctt tcggaatcac gaaagaatcc
     3421 acccaaatcc ccacgccctc tttgggcatc atcacgcgga ttttttcctt gccgcccgct
     3481 tcttcggcac ggcgtttggc gatgttcaaa tcgccgccga aaccgattgt tacgcaggta
     3541 tcgccgcgcg ccaaatcatc gataaagccg gacgaagtaa agcgtttgat attggggcgg
     3601 tttttcttga gcagggcggt tgcctccctg atgtcttccg tattgctgct gttcgggttt
     3661 ttacccaaat agttcaacac cataggatag atttccgccg cgctgtctaa ataactgata
     3721 ccgcattgtt tgagtttgga cgtgtattcg gggtcgaaca ccaaatccca ctggttgtcc
     3781 ggcagcttgt ccgtacccaa agcctttttc acgcgttcgg tattgatggc gaaggtattt
     3841 gtcccccaat aaaacggcac ggcgtattcg tggccgggat cgaccccgtc catcagcctc
     3901 atcatttcgg gattgaggtg tttataattg ggaatcagcg atttatcgat tttctgatac
     3961 gcacctgcct taatctgcct gcccacaaac gcattggacg gcgcgacaat gtcgtaaccg
     4021 gattttccgg tcagcacctt gctttccagc gtttcatcgc tgtcgtacac atcataagta
     4081 accttgatgc cgtttttctt ttcaaaatcg gcaacggttt ccggatcgac gtattccgac
     4141 cagttgtaaa ttttcaatac gtttcggttt tccgccggcg ccggtttttc ggcaggcggt
     4201 ttgtccgaac cgccgcacgc tgcaagcagc aaagcagtca ggacggcgag tggcagatgt
     4261 ttggccatta tcattccttg catatcgggt tggagaaagc ggtcattata gccgatattg
     4321 gcaacagggc ttcagacggc attcaaaatc ccgccacacc cttccgaaaa ccgccgcttc
     4381 catagctaga aacagggatt tgcggtaaga taccgccgtt cgttttccct gcttttacca
     4441 tgacaagaca tttgagagac attgaaaaaa ttatgaaaac ctccgaactg cgccaaaaat
     4501 tcctaaaatt ttttgaaacc aaaggccaca ccgtcgtccg ctcttccagc ctcgtgccgc
     4561 acgacgaccc gaccctgctg tttaccaacg cgggcatgaa ccagtttaaa gacgtattct
     4621 taggtttcga caaacgcccg tacagccgcg ccaccaccgc gcaaaaatgc gtacgcgcag
     4681 gcggcaagca caacgacttg gaaaacgtcg gctacaccgc ccgccaccac accttctttg
     4741 aaatgatggg caacttctcc ttcggcgact acttcaagcg cgacgcgatc cacttcgctt
     4801 gggaatttct gacttccccc gaatggctca atatccccaa agacaaactg ttggcgaccg
     4861 tttacgcgga agacgacgaa gcctacaaca tctggttgaa cgaaatcggt atgccgtccg
     4921 agcgcatcgt ccgcatcggc gacaacaaag gcgcgaaata cgcgtccgac aacttctggc
     4981 aaatgggcga caccggccct tgcggcccct gctccgaaat tttctacgac cacggcgaag
     5041 aaatttgggg cggcattccc ggcagccccg aagaagacgg cgaccgctgg atcgaaattt
     5101 ggaactgcgt attcatgcag ttcaaccgcg acgaacaagg caatatgaat ccgctgccca
     5161 agccgtccgt cgataccggc atgggcttgg aacgcatggc cgccgttatg cagcatgttc
     5221 acagcaacta cgaaatcgac ttgttccaag acctgctcaa agccgttgcc cgcgaaaccg
     5281 gcgcagcttt cagcatggaa gaaccaagcc taaaagtcat cgccgaccac atccgctcct
     5341 gctcattcct gattgcagac ggcgtattgc cttccaacga aggccgcggc tacgtattgc
     5401 gccgcattat ccgccgcgcc gtgcgccacg gttacaaact gggtcaaagc aaaccgttct
     5461 tccacaaact ggtggccgat ttggtcaaag agatgggcga tgcctaccct gaattgaaag
     5521 aaaaacaagt tcaaatcgaa gaagcgttga aaaacgaaga aagccgtttt gcccaaactc
     5581 tggaaaccgg tatggctttg ttggaaaacg cgcttgccaa aggcagcaaa aaactcgacg
     5641 gcgaaatcat cttcaaactc tacgatacct acggtttccc atacgacttg actgccgaca
     5701 tctgccgcga acgcaatatc gaactggacg aagcaggctt cgagcgcgaa atggaagccc
     5761 aacgcgcacg cgcacgcgcc gcacaaagct tcaaagccaa cgcccaactg ccttatgacg
     5821 gtcaagacac cgagtttaaa ggttatagcg aacgccaaac cgaatccaaa gtcctcgccc
     5881 tctacaaaga cggcgagcaa gtcgacgaat tgaacgaagg cgacagcggc gcagtcgtca
     5941 tcgactttac cccgttctat gcagaatccg gcggccaagt cggcgatgtc ggctatatct
     6001 tctcaggcga aaaccgcttt gaagtacgcg atacccaaaa aatcaaagcg gccgtattcg
     6061 gtcaattcgg cgtacaaact tcaggccgtc tgaaagtcgg cgacagcgtt accgccaaag
     6121 tggacgacga aatccgcaat gccaatatgc gcaaccacag cgcaacccac ttgatgcaca
     6181 aagccctgcg cgatgtattg ggcagacacg tcgaacaaaa aggctctttg gttaccgccg
     6241 aatccacccg tttcgacatt tcccatcccc aagcggtaac tgccgaagaa atcgccgaag
     6301 tcgaacgccg cgtcaacgaa gccgttttgg cgaacgttgc cgtcaacgcc gccattatga
     6361 gcatggaaga cgcgcaaaaa accggcgcca tgatgctctt cggcgaaaaa tatggcgacg
     6421 aagtacgcgt attgcaaatg ggcggtttct ctaccgaatt gtgcggcggc acgcacgttt
     6481 cacgcaccgg cgacatcggc ctcttcaaaa tcatcagcga aggcggtatt gccgcaggcg
     6541 tgcgccgtat cgaagccatc accggcctga acgcactcaa atgggcgcaa gagcaagagc
     6601 gtttggtgaa agacatcatt gctgaaacca aagcccaaac cgaaaaagac gtactggcga
     6661 aaatccaagc aggcgcggca cacgccaaag cattggaaaa agaattggca cgcgccaaag
     6721 ccgaactcgc cgtccacgca ggcgcaaaac tcttggacga cgcaaaagac ttgggcgcag
     6781 ccaaactcgt tgccgcccaa atcgaagccg acgcagccgc cctgcgcgaa atcgtaaccg
     6841 acctgaccgg aaaatccgac aacgccgtga ttcttttagc ggcagtaaac gacggcaaag
     6901 tctccctgtg cgccggcgta tccaaagccc tgaccggcaa agtcaaagca ggcgatctgg
     6961 ttaaatttgc agccgaacaa gtcggcggca aaggaggcgg cagaccagat ttggcacagg
     7021 cagggggcac ggatgccggc aaattgcccg ccgtgttgga tagcgtgaaa gactgggtcg
     7081 gcgcgaagct ggtttgatgt gggaaaggca gcctgaaagg gattcaggct gccttttgtg
     7141 ttaaagatgc cgtctgaaag atattttcag acggcctctg tcagtttatt tggctttata
     7201 cctaccattg aatagcgatt cagaaggtat ccagccatct gccaaatcat ccaacatctt
     7261 aatccaatac tctaggcgtt tttctgtcgg gacgctttct ccccaatatt cgacaattag
     7321 agcatcatcc atttccaaag cagctttttc aatttcatgc tcaatcaatt ttaaagattt
     7381 ttctacatag tttccgccac ggtagtcttc cgccgagttg gcaacgctgc ccgcactgaa
     7441 taagtcccgc gcctgatgac aaagcacacc gcgataggta atcagataca ggacggcatt
     7501 atcgtatttg tgcttcgccc tgcctgagca gacaatacgg ggggagacta atgctgcttc
     7561 agctcggagg cggatttttt cttcctgcgg cagggaagtg taaaggcgca cttccaaagg
     7621 cagatcatgg ctgtctactg cgtcaatgtc ttctatccac cataaccgct cgccttcttt
     7681 cagcctgtta cgggcatatt ttcggatata ttccattttt tcatgcatat ccaaatcggc
     7741 aaattcagca taggaaatac cgaaaccctc aaacagagac ggtcgatcag attctatttc
     7801 tacctcaaag cgcggaacat gcgatgtgcg gacatgaatt acgctatctt cgtaaagttg
     7861 ccatttcaca tcgggaatgc cccccatttt tccgaatacc acatagatat gctgtacacc
     7921 gtcagctttg tttttctctt ggatactgtt tgctacgctg cgccaagtat cttttagcgt
     7981 gaatttgact tctacaccat attcccctat tggaacatcg gggaaaattt gcggcacagg
     8041 ttcaggattg ataactatgt cagcataagc gggaattttt gcaatgcaca gcgcacgaac
     8101 tctgttttca aaaatatctt gggatttgaa aggtgcgttt tttgcctctt gcctcaatac
     8161 ggtgcaaacc tcgtctaaaa acttctcaaa ctcctttttt gtcagcataa tgcattacca
     8221 cttaaaaata taaagtagta attttatctt atttaacagg gttatttaag tgccttacta
     8281 acggcggcgg cgatgtgcca ccctaaaaca ggagggacgg cgttgccaat ttgccgttct
     8341 gtggcacgca attttgcctg gaaaataaaa ttgtcgggaa acgattggat gcgcgccgcc
     8401 tcacgcatgg agatgcggcg gggtaattcg taatgccact gtatgtttcc atggcattct
     8461 gccctaatag tgtagcccgc cctttcggca tccaattttc gactaccttg atcggggctt
     8521 ttttctgctc tactccaaat atggttggca atttcgtctt ctactttgtc gaccaaatcg
     8581 cctatagcct gtctagctgt tacatagctg ttgctggtat ttgttttttc aatctgtatt
     8641 tcgggaattt caggtagcct tccaacgatg ataactcgct cccgagtctg tggaacaccg
     8701 taatcggctg cattcagcaa gtagtaatct acctgataac ccaaattgcg aaaatctgaa
     8761 agcactgttt ccaaatcttt ccgatgatgc ttcatcagta gccctttgac attttcagca
     8821 acaaatattt tggggcgaac gcgttcaaca gcagacaaca tggctttata cagcccgctg
     8881 cggctgcctg aaatacctgc acctttacca ttaatagaaa tatcctgaca aggaaagcca
     8941 ccgataatga tgtctgcgct agtgggtatt tcatccaaat gctgccaaat atcaccatgt
     9001 acaatgtgat ttcctaagtt gtggcggtag gttctaacag cgtgttcgtc gatttcgttc
     9061 gcccaaataa tatcaaaatc atgtttgtca taagtacggt ttaaaaactc aaagcccccc
     9121 agaaaaccca aatccattcc gccacaaccg ctaaataaag aaataatttt atattttttt
     9181 tcgcggtctt cttgcttttc ctgtttggtc attaactcca tacatttaag ataaaaagca
     9241 tgggaaaaag tacctctatt tactttgtta caaattgcat ttgccgtttc actttcccct
     9301 tgctccaaga ataattctga taattgtata aagccaattc catgctcatg cattttggct
     9361 tttatggcat ctccagctaa cttgttccaa ttttttcgtg caaattcggt catggttaat
     9421 ctttcagtca ttcataaatg acattatttt agcgtttttt ataatttaag ccaagattta
     9481 aaatctacct ttaggtttac aggccgtctg aaaacaaaaa tctcgttttc agacgacctt
     9541 tttccgctaa aatcccccta ccgtccaacc cgaaacaagg agaaccatca tgcaagttcc
     9601 tacttccgcc atcatcaacg gcgaatttgc cgacaaatac ggcaaacgcg gcagcaagcc
     9661 gtagccagca cccctgtcaa ccgcgtgcgt ggctagacca cacaccctac cactgcgggc
     9721 gacgcaaacc ttaagattga ggtgtagggt gtgtgcggta cgcacgcacg cggtttttgt
     9781 tactaccgaa tgattccaaa ccatgccgta atccagtcag cagcaaacgc tgcttgggtc
     9841 atgtaccaac aaaactcaat atcaaaggcc gtctgaagaa taattttcag acggcttctc
     9901 tctatcaaaa gcagcctgca cattcaaaaa tcgaagtgca ggctgctttt tgtgatgatt
     9961 ggaaaaaaca cacactattt gagacctttg caaaattccc caaaatcccc taaattccca
    10021 ccaagacatt taggggattt ctcatgagca ccttcttcca gcaaaccgcc caagccatga
    10081 ttgccagaca catcgaccg
//
LOCUS       DAWFAD010000061         9925 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWFAD010000061 DAWFAD010000000
VERSION     DAWFAD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.061"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(112..1554)
                     /gene="aldA"
                     /locus_tag="WGO49_001143"
     CDS             complement(112..1554)
                     /gene="aldA"
                     /locus_tag="WGO49_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ0306255.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVVAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1589..1789)
                     /locus_tag="WGO49_001144"
     CDS             complement(1589..1789)
                     /locus_tag="WGO49_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306256.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTGANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTVIYLGIN"
     gene            complement(1798..2187)
                     /locus_tag="WGO49_001145"
     CDS             complement(1798..2187)
                     /locus_tag="WGO49_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306257.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLIN
                     YSYLDNCLVTLTVDRISVSFIREIQAA"
     gene            complement(2384..3244)
                     /locus_tag="WGO49_001146"
     CDS             complement(2384..3244)
                     /locus_tag="WGO49_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ0306258.1"
                     /translation="MEGSYRFDTLSNGISIHGGTVTARCDFCSSRLAEPYVSFVLLLE
                     GSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRYLYRGGKTVKMTIKGMEQWLLRPE
                     YARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPKGHLGETLRREADVLRLLSDLWDT
                     VSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGAHQVNRLTDALNISERTLQRRMRD
                     HFGITASEWLHHKQMQHALYLLQNGGKSIGETAYFMRLPPRFQLYSGIQAIFRQHAGG
                     NQKRKPISRI"
     gene            3486..4286
                     /locus_tag="WGO49_001147"
     CDS             3486..4286
                     /locus_tag="WGO49_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0306259.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSVMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4335..5111
                     /gene="mlaE"
                     /locus_tag="WGO49_001148"
     CDS             4335..5111
                     /gene="mlaE"
                     /locus_tag="WGO49_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ0306260.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVTWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5162..5656
                     /gene="mlaD"
                     /locus_tag="WGO49_001149"
     CDS             5162..5656
                     /gene="mlaD"
                     /locus_tag="WGO49_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ0306261.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVGFLAFRVAGGAAFGGSDKTYAVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5693..6283
                     /locus_tag="WGO49_001150"
     CDS             5693..6283
                     /locus_tag="WGO49_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ0306262.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            6340..6618
                     /locus_tag="WGO49_001151"
     CDS             6340..6618
                     /locus_tag="WGO49_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ0306263.1"
                     /translation="MHTELNNGTLHISGDITVKTLTGEAFERFQQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6615..7442
                     /locus_tag="WGO49_001152"
     CDS             6615..7442
                     /locus_tag="WGO49_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ0306264.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7567..7929
                     /locus_tag="WGO49_001153"
     CDS             7567..7929
                     /locus_tag="WGO49_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ0306265.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7926..8312
                     /locus_tag="WGO49_001154"
     CDS             7926..8312
                     /locus_tag="WGO49_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0306266.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            8309..8797
                     /locus_tag="WGO49_001155"
     CDS             8309..8797
                     /locus_tag="WGO49_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0306267.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLSDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKVIKTANDYKNKDFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVIYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8881..9048)
                     /locus_tag="WGO49_001156"
     CDS             complement(8881..9048)
                     /locus_tag="WGO49_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0306268.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(9015..9395)
                     /locus_tag="WGO49_001157"
     CDS             complement(9015..9395)
                     /locus_tag="WGO49_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ0306269.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGTNCEICRGLVVGKNVMMEPECLFYSNNHKFDHS
                     KNALRATRKSVRLRWRTMSGRGTG"
     gene            9405..9531
                     /locus_tag="WGO49_001158"
                     /pseudo
     CDS             9405..9531
                     /locus_tag="WGO49_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            9633..9848
                     /gene="rpmE"
                     /locus_tag="WGO49_001159"
     CDS             9633..9848
                     /gene="rpmE"
                     /locus_tag="WGO49_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ0306270.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
BASE COUNT         2323 a         2754 c         2485 g         2363 t
ORIGIN      
        1 gacaaaaaca tcgatgtcgg tcatgactga ttgccggtga aactgtaaaa atgccgtctg
       61 aacctgaaaa cgggttcaga cggcattttc tatcggggtt ttaaagcggc attaaatgtc
      121 ggtttccaga taaacgactt gggtttgcag atattcttcc aaaccgtgtt tgccgtccgc
      181 gccgccgatg ccggattttt tccagcccgc gtggaaaccc tgcatggctt cgaagttttc
      241 gcggttgatg taggtttcgc cgaattgcag gcggcgggta acgtagaagg cttcgtttaa
      301 attagtcgta taaacagaac tggtcagacc aaactcgcaa tcgttagcca gtgcgatgac
      361 ttggtcgagc gtgtcgaaag cggaaacggg cagcacgggg ccgaaggttt cttctttcat
      421 aatgtccata ctgttgtcgg tgtcggtcag cagggtcggc tcgaagaaat agccgcgccc
      481 ttcggcgcgt ttgccgccgc agaccagttt cgcaccttgt ttgactgccc gttccacttt
      541 ttcggcaacg gctttgacgg cgcgctcttc aatcagcgga cccatttcca gcgcgcctgc
      601 ttcggcttcg gcagggttgc cgtagcgcac gcctttcatg gcggcggtca ttttttcaat
      661 gaacgcgtct ttcaggctgc tgtggacata gacgcgctcg gcgcagttgc agatttgacc
      721 ggtgttgccg acgcgcgaag ccaagatgga tttcaccgcc aaatccaaat ccgcgtcttt
      781 caaaacgatg gcgggggctt tgccgccgag ttccaacgaa actttggtga tgttggcgga
      841 ggcggcttcc atcacttggc ggcctgcttc gacggagccg gtcaggctga ccatatcgac
      901 ttgcggatgg gcggacaagg cattgccgat ttccgcacca gggccgttca ctacgttgaa
      961 cacgcctgcg ggcagtccga ccgcatcgac gatttcggcg aagatgtggc agttgatcgg
     1021 ggttacgctg ctgggtttga cgacgatggt attgcccgtt accaaagccg ggcccatttt
     1081 gcgggcaatc aggaagaagg ggaagttcca cggcaaaatg cctgccacga cgcccagcgg
     1141 acgtttgaac aataaaatgt tttcgcgcgg gcggtcgctt tggatgattt cgccttcgta
     1201 acggcgcgcc cattcggctt ggtaatcgag ataatcggca gtgaacatga cttccacgcg
     1261 cgccaagtct ttggttttgc cgccttcggc aacgatggtg tcggtcagct cgtcggcgcg
     1321 ttcgcgtatg ccttgggcga ttttgcgcaa atacgcgccg cgttcgaccg caggcagacg
     1381 ctcccaagcc ggttgcgccg cacgcgccgc cgcgacggcg cggtcaacgt ccgccttgcc
     1441 gcctttgggt tcgcgggcga tggtttcttc ggtggatggg ttcaatacgt cgcgccattc
     1501 gccgttgaaa tcgttttcaa agcgtccgtt gatgtacatg gccaattgtt tcatttcggg
     1561 ttctccagtt ttgtagtcag atgtagtttt agtttattcc caaataaatt accgtacaag
     1621 tttcttttga aatgtttaga cgagaagcta ctatgtctgc atattgttct gctaatttat
     1681 attcactcga atgcaactgc cgattattcc aatctgcatt cgcaccagta aaagccccag
     1741 cattgccgcc tgtggcataa ccaatcgcta aactgccaac ggtatccaca acaggtttca
     1801 ggctgcctga atttctctaa taaatgaaac acttattcta tcaaccgtta atgtaaccaa
     1861 acaattatca agataagagt agtttattaa atactcatct tcttcaaatt gtatattttg
     1921 tagggttaga tgattatttt ttaattctgc cgttatttca tacttgaaac tatcatctat
     1981 ttttctaaat ccaatatttg aagttgattt atccagtatg taatcattaa agatttcata
     2041 ggataattcc acttcataaa attgattttc aaatattttg aatgggcagt acgatataaa
     2101 acaatgtagt tcataaccat ttatttcaat caatactgcc tcttcaatgg tgttatctaa
     2161 ctttttgatt ttacatttat ataacattta tcttgccttt ggataaattg ttttgttttt
     2221 tggattgtta ttcaaagttc tctaaaagtc ttttcctgat tttgtatgta tcgtggtaat
     2281 actaccattt tcattggttc caacaaaaat aaatttagat tgacctttgg aatcagtacc
     2341 caaaaactga acatatccag acatgcggat ttcgggtttt aaatcaaatg cggcttatcg
     2401 gttttctttt ttggtttccg ccggcgtgct gccgaaatat tgcctgaatg cctgagtaaa
     2461 gctggaaacg tggcggtagc cgcataaaat atgcggtttc gcctatgctt ttgcccccgt
     2521 tttgcaacag gtagagcgcg tgctgcattt gtttgtggtg cagccactcg cttgcggtaa
     2581 taccgaaatg gtcgcgcata cggcgttgca gcgtcctttc actgatgttc agcgcgtctg
     2641 tcagccggtt gacttggtgt gcgccgtcgg caaacgcggc atttagggtg cggctgaagt
     2701 cttcagacgg catagcgtct gcttccgccg tttgccccgc cgccggcccg atgccgtctg
     2761 aaaccgtgtc ccacaagtcc gacagcagcc gcaacacgtc cgcctcgcgg cgcaatgttt
     2821 cgcccaaatg cccctttggg acggctttca ggcaggatgc cgccaagccg cgcaggttcg
     2881 ggggcaaatc ccatatcctg accggttcgc ggtaaagcag gggtgcgaaa cgcgcgtatt
     2941 ccggacgcag cagccattgt tccatacctt taatggtcat tttgaccgtt ttgccgcctc
     3001 ggtaaagata gcggctgaac aggacttctt ccccgacagc aattaggaca atcttgccgc
     3061 cgtccgcatc gatttggaag cggcagcggt tgatgccgaa gtccaaactg ccttccagca
     3121 agagcacgaa cgacacataa ggttcggcga ggcggctgct gcaaaaatca caccgtgccg
     3181 ttactgtgcc gccgtggatg gaaatgccgt tggacaaagt atcgaaacgg tagctgccct
     3241 ccacatagct gccgccgccg cttttgacta gattgacgaa ccggctgctg ttcaaatgtt
     3301 ctgctttttc catagttttc tgataaaggt tatcatttga aagataacat ttttcgccac
     3361 agcaaacaat ccgccgccga caacggcatt ttcacaacat ttaccgtccg tatgccgtct
     3421 gaaaattgcc cgtcttcccg ataagcctta aaatacacgc cgtcaaacct accgtccgtc
     3481 cgtacatgag cccatccccc tttatcgaaa tgaaagacgt cgccttcgcg tatggcgacc
     3541 gcccgattct gaagaacatc aatttcagca ttccgcaagg caattttgct gccgtaatgg
     3601 gcggttcggg cagcggcaaa accacgctga tgcgactgat tacaggacag attcgtccgc
     3661 agtccgggca ggttttgatt gaaggacggg atttggcggg cttttcggct gacgagttgt
     3721 atgaacaccg ccgccgtatg ggcgtattat tccaacacgg cgcgctgttt accgatttgt
     3781 cggtattcga caatatcgct tttccgatgc gcgaactgac gcgcctgccg gaagcggtga
     3841 ttcgagattt ggttttgttg aaactgaacg cggtcggttt gcgcggcgtg gaaaacctga
     3901 tgccgtccga gttgtccggc gggatgtcgc gccgcgtcgc gcttgcccgc acgattgcgc
     3961 tcgaccctga aattatgttg tacgacgaac cgtttaccgg cctcgatccg atttccttgg
     4021 gcgtgattgc ccacttgatc agccgcgtca ataaggcttt gcgttcgacc agcgttatgg
     4081 taacgcacga cattgaaaaa tctttggaaa tcgtcgatca ggtgattttc ttggcgcacg
     4141 gcgaaattat gttctccggc tcgccgcagg aaatgcgcga actggattcg ccttgggtgc
     4201 gccagtttgt cggcgggctg gcagacggcc ccgtagcata ccgttatccg gcgcaaacgt
     4261 cgttgcagca ggatttgctc gggtaaattg agccggtgcc gtctgaaacc gttgaacctt
     4321 acatatagaa atttatgaac tttatccgtt ccgtcggggc gaaaaccctc ggccttattc
     4381 aatccctcgg cagtatcacg ctgtttctgc tgaacattct ggcgaagtcc ggtacggctt
     4441 tcgtccgtcc gcgcctgagc gtgcgccaag tgtatttcgc cggcgtgctg tcggtgctga
     4501 ttgttgccgt ttcggggctg ttcgtcggta tggttttggg tttgcagggc tatacgcagt
     4561 tgtcgaaatt caaatccgcc gatattttgg gctatatggt tgcggcttcg ctgttgcgcg
     4621 aactgggtcc ggtattggcg gcgattctgt ttgccagcag cgcgggcggt gcgatgacca
     4681 gcgaaatcgg tctgatgaaa acgactgaac agctcgaagc gatgaatgtg atggcggtaa
     4741 accccgtcgc ccgcgtggtt gcgccgcgct tttgggcggg cgtgttttcc atgccgcttt
     4801 tggcttcgat tttcaacgtg gcgggtattt tcggcgcgta tttggtcggt gtaacctggc
     4861 tgggcttgga cagcggcatt ttctggtcgc aaatgcagaa caacatcacg atacattacg
     4921 atgtaatcaa cggtctgatc aaatccgccg cgttcggcgt ggcggtaacg ctgattgccg
     4981 tgcatcaggg cttccactgc gtcccgacct cggaaggcat tttgcgcgcc agcacgcgca
     5041 cggtggtttc gtccgccctg acgattttgg cggtcgattt tatattgacc gcgtggatgt
     5101 ttacagattg acagggcgtc tgaagacgaa acgcggaaca tcggatattc aaggaacttt
     5161 aatgaaaaag aacatattgg aattttgggt cggactgttc gtcctgattg gcgcggcggc
     5221 ggtcggcttt ctcgctttcc gcgtggcggg cggtgcggcg ttcggcggtt cggacaaaac
     5281 ttacgccgtt tatgccgatt tcggcgacat cggcggtttg aaggtcaatg cccccgtcaa
     5341 atccgcaggc gtattggtcg ggcgcgtcgg cgctatcgga cttgacccga aatcctatca
     5401 ggcgagggtg cgcctcgatt tggacggcaa gtatcagttc agcagcgacg tttccgcgca
     5461 aatcctgact tcgggacttt tgggcgagca gtacatcggg ctgcagcagg gcggcgacac
     5521 ggaaaacctt gctgccggcg acaccatctc cgtaaccagt tctgcaatgg ttctggaaaa
     5581 ccttatcggc aaattcatga cgagttttgc cgagaaaaat gccgacggcg gcaatgcgga
     5641 aaaagccgcc gaataaacgt atgccatcat attcaaacta ggacacttaa tcatgaaaaa
     5701 atcctccctc atcagcgcat tgggcatcgg tattttgagc atcggcatgg catttgccgc
     5761 ccctgccgac gcggtaaacc aaatccgtca aaacgccact caagtattga gcatcttaaa
     5821 aagcggtgat gccaacaccg cccgccaaaa agccgaagcc tatgcgattc cctatttcga
     5881 tttccaacgt atgaccgcat tggcggtcgg caacccttgg cgcaccgcgt ccgacgcgca
     5941 aaaacaagcg ttggccaaag aatttcaaac cctgctgatc cgcacctatt ccggcacgat
     6001 gctgaaatta aaaaacgcca acgtcaacgt caaagacaat cccatcgtca ataaaggcgg
     6061 caaagaaatc atcgtccgcg ccgaagtcgg cgtacctggg caaaaacccg tcaacatgga
     6121 cttcaccacc taccaaagcg gcggtaaata ccgtacctac aacgtcgcca tcgaaggcgc
     6181 gagcctggtt accgtgtacc gcaaccaatt cggcgaaatt atcaaagcga aaggcgtgga
     6241 cggactgatt gccgagttga aggctaaaaa cggcagcaag taatccataa gccatgccgc
     6301 cagactgaag atggcatgga ttttttccta ccgaacacta tgcatacaga actcaacaac
     6361 ggaacactgc acatcagcgg cgacatcacc gtcaaaaccc tgaccgggga ggcttttgag
     6421 cgtttccagc agcaatgccg tctgaaagaa accattgccg tcgatttcgg cggtgtcaaa
     6481 cgtgccgatt ccgcctgcgt gtcgctgctg ctcgaagtgc tgcgcggttg caaaggcagc
     6541 gttaggctga ccggcattcc cgaatccgtg cgtgcgctgt ccgaactgta cgaaatcaaa
     6601 gactggctga aatcatgaaa aaaaccgcct atgccttcct cctgctgatc gggttcgctt
     6661 ccgcccctgc atttgccgaa acccgccccg ccgaccctta tgaaggctac aaccgcgccg
     6721 ttttcaaatt caacgaccaa gccgaccgct acattttcgc ccctgccgcg cgcggctacc
     6781 gcaaagttgc gccgaaaccc gtccgcgccg gcgtgtccaa tttttttaac aacctgtgcg
     6841 acgtggtcag cttcggcagc aatatcttgc gcttagacat caaacgcgca agcgaagacc
     6901 ttgtccgcgt cggcatcaac accactttcg gtttgggcgg gcttatcgac atcgccggcg
     6961 cgggcggcat tcccgacaat aaaaacacct tgggcgacac gtttgcttcg tggggatgga
     7021 aaaacagcaa ttatttcgtg ttgcccgtct tagggccgtc caccgtccgc gacgcgctcg
     7081 gcacgggtat tacctccgtt tattcgccca agaatatcgt cttccgcacc cctgtcggac
     7141 gctggggcac gactgccgta tccgccgtca gtacgcgcga aggcctgctc gatttgaccg
     7201 acagtctgga cgaagccgcc atcgacaaat acagctacac gcgcgacctc tatatgaaag
     7261 tccgtgcgcg gcagaccggt gcaacacctg ccgaaggtac ggaagataac atcgacatcg
     7321 acgaattggt cgaaagtgcc gaaaccggcg cggcggaaac tgccgttcaa gaagattccg
     7381 tatccgaaac acaggcagaa gcagcagggg aagccgaaac gcaacctgga acacaacctt
     7441 aaagccatgc cgtctgaaaa tccgtgttcg gacggcattc ccttctgtcg gaacggacag
     7501 tccgaaacca cagattgggc tataatctct tttttgcgca taccgcgcga cagcaaagga
     7561 aaaaccatgt acgaagtcaa ccgcagcgta ttcgtcctca tccctttaga acctttttgg
     7621 aactggctgc aaaccctgcc gggcaaccac ctcgacggac tgactcttga agacatccaa
     7681 gccgacgcca attcctacct tgtccgcccg tgcgaaaccg ccgacgaagt atgggacgaa
     7741 atcgaagccc gttttgaaga cattttcgcc gccgaacttg ccgactggtg cgaagacgaa
     7801 cgcgagtggc ctgcgctcga tgccgacatt ttcaacgaat ggttcgacat ccagctttct
     7861 accgtcatca ccgacctcga acacgaaccg cttgcccgcg aagccttcca acccatcaac
     7921 ctgaactgat gaagctgacc gtccgcaact accatctcga cggctacggc catgtcaaca
     7981 atgcgcgcta cctcgaattt ttcgaagaag cgcgctgggc gttttttgaa gaacgcggac
     8041 tgctgcacga gttggcaggc ctcatactga ttgtcgcccg catcgacatc cgatacagcc
     8101 gccccgccgt cgaaggcgac gtattgcagt tttcatgccg cctgaaaacc cccggcacgc
     8161 gccgtattgt gctgacccag acgattacac tgccaaacgg caaaaccgcc gcagaagccg
     8221 acatcaccct gatgcccgtc cacgccgcca cacaacgcac cgtcagcctg cccgccaccc
     8281 ttgcccgcgc attggaagcc ttgtccgaat gaaaaaaata ctcaccgccg ccgtcgtcgc
     8341 actgatcggc atcctccttg ccatcgtcct catccccgac agcaaaaccg cgcccgcttt
     8401 ctccctgtcc gacctgcacg gaaaaaccgt ttccaacgcc gacctgcaag gcaaagtaac
     8461 cctgattaat ttttggtttc cctcctgtcc cggttgcgtg agcgaaatgc ccaaagtcat
     8521 caaaacggca aacgactaca aaaataaaga tttccaagtc cttgccgtcg cccagcccat
     8581 cgatccgata gaaagcgtcc gccaatacgt caaagactac ggactgccgt ttaccgtcat
     8641 ttatgatgcg gacaaagccg tcggacaggc attcggcaca caggtttatc cgacttccgt
     8701 ccttatcggc aaaaaaggcg aaatcctcaa aacctacgtc ggcgaacccg atttcggcaa
     8761 actctaccaa gaaatcgata ccgcgctggc acaatagcga tgccgtctga agcgcaaaaa
     8821 aaaacagaaa cgatgttcct gttttttcag atcatccgcc ccgacacgtt cagacggcat
     8881 tcaaccttcc ggcagattct ttttcaccac tgccggattg cctgccgcca aagagtaggg
     8941 cggaatgtct tttgtaacca ccgcgcctgc gcccacgacc gaaccgcgtc cgacggttac
     9001 gcccgccatt acaatcaccc tgtgccccgg ccagacatcg tcctccaacg taatcggacg
     9061 gatttccgtg tagccctcaa agcgtttttt gaatggtcaa acttgtggtt atttgaataa
     9121 aacagacatt ccggctccat catcacattt ttgccgacca ccagcccacg gcagatttca
     9181 cagtttgtcc cgatgcccga gccgtcgccc aaaaccgtat ccggaaacac atacgccccg
     9241 cgttcgatat tgaccccgcg tccgatatgc ggagaaaccc gccgcgccaa aaaaccgcgc
     9301 acccgtctgc cgacgatgcc gatgccgcgc atatgtgacg gcggcaaccc cgaaccggca
     9361 aaacgcagca gcaggaaaaa gaattttgat cccatcaaac ctccctcaaa agaacttttt
     9421 atagtggatt aacaaaaatt aggacaaggc aacgaagccg cagacagtac agatagtacg
     9481 gcaaggcgag gcaacgctgt actggttttt gttaatccac tatacatttt atcgaatata
     9541 ctcttgacgt aacaacagaa aatagagaga atgtcggact tgtctgtagc ataggtacat
     9601 cccctatgcc cgcttttttg aaaggaaatc aaatgaaaca aggtattcac ccgaactacc
     9661 acgaagtcaa cgttacctgc tcttgcggca acaaattcgc aaccaaatcc gcgatggaaa
     9721 aagaaaactt caatatcgag gtttgctccc tgtgccaccc gttctatacc ggcacccaaa
     9781 aaatcgttga taccaccggc cgcgtggaca aattcaacaa caaattcggc aacctgttca
     9841 aacgctgatt gccgctttgc aagaattaag ccctgccatc atttcgatgg cagggctttt
     9901 taatatttgg cacactgact tctta
//
LOCUS       DAWFAD010000062         9910 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWFAD010000062 DAWFAD010000000
VERSION     DAWFAD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.062"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(36..761)
                     /locus_tag="WGO49_001160"
     CDS             complement(36..761)
                     /locus_tag="WGO49_001160"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ0306271.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSRDLDAGRLLEAYRN
                     GVFPWFLRDGWFFWYAVGPRAVIVPERLHVPRSLEKTLRNCSYRVTVNGCFAEVVAHC
                     AAAVRPNQDGTWIAPEFQTAYLKLHEMGYAHSFECHYPDENGKTRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLASLGVELIDCQQDTGHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(831..1334)
                     /gene="fur"
                     /locus_tag="WGO49_001161"
     CDS             complement(831..1334)
                     /gene="fur"
                     /locus_tag="WGO49_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ0306272.1"
                     /translation="MIVGKQAEIAYYSVYYFTGILNIMEKFSNIAQLKDSGLKVTGPR
                     LKILDLFETHAEEHLSAEDVYRILLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGK
                     AVYELDKGDHHDHIVCVKCGEVTEFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSD
                     CQAKGKR"
     gene            1492..1869
                     /locus_tag="WGO49_001162"
     CDS             1492..1869
                     /locus_tag="WGO49_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0306273.1"
                     /translation="MNKTLILVLSALFSLTACSVERVSLFPSYKLKIIQGNELEPRAV
                     AALRPGMTKDQVLLLLGSPMLRDAFHTDRWDYTFNTSRNGIIKERSNLTVYFENGVLV
                     RAEGDALQNAAEALRAKQNADKQ"
     gene            1879..2688
                     /gene="dapB"
                     /locus_tag="WGO49_001163"
     CDS             1879..2688
                     /gene="dapB"
                     /locus_tag="WGO49_001163"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ0306274.1"
                     /translation="MIPLKIAIAGVNGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDTVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIQAAGEKTGVVFAANFSVGVNLTFHLLDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKASSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2952..3362)
                     /locus_tag="WGO49_001164"
     CDS             complement(2952..3362)
                     /locus_tag="WGO49_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306275.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGSRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            3585..3974
                     /locus_tag="WGO49_001165"
     CDS             3585..3974
                     /locus_tag="WGO49_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0306276.1"
                     /translation="MNVKIKYNLLEYCEYLGGIFGVYKNYLNMDIKDPNLKIRFFDFL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRRIWPDMEEMLLYFPDNPWFYVEHF
                     WWGATCPIELTQLPKIEIYEEQMKQGK"
     gene            complement(4018..4176)
                     /locus_tag="WGO49_001166"
     CDS             complement(4018..4176)
                     /locus_tag="WGO49_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306277.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGQLTD"
     gene            complement(4184..5338)
                     /gene="lpxB"
                     /locus_tag="WGO49_001167"
     CDS             complement(4184..5338)
                     /gene="lpxB"
                     /locus_tag="WGO49_001167"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ0306278.1"
                     /translation="MVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARFVGIGGE
                     LMKAEGFESLYDQERLAVRGFAEVVRRLPEILRIRRGLVRDLLSLKPDVFVGIDAPDF
                     NLGVAERLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLYLDAGGR
                     AEFVGHPMAQLMPLEDDRETARKTLGVDAGIPVFALLPGSRVSEIDYMAPVFFQTALL
                     LLERYPAARFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAADAVLVTS
                     GTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPELLQSEAK
                     PEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(5404..6396)
                     /locus_tag="WGO49_001168"
     CDS             complement(5404..6396)
                     /locus_tag="WGO49_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0306279.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVSKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPLDLAQFAVMLENGTKM"
     gene            6889..9648
                     /locus_tag="WGO49_001169"
     CDS             6889..9648
                     /locus_tag="WGO49_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ0306280.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFRDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVHDQVAEFMSYVMPGNVGRLKLYQDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGSDIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVHAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRIDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGSEKAKASRPEPAVKGVRHTQPAPTVAPEKKASWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTTNRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAGRADEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRSE
                     RGQTAPETATVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSETATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEVATVPAEEMIQV
                     ETRQG"
BASE COUNT         2559 a         2990 c         2303 g         2058 t
ORIGIN      
        1 accatatttg aacctgaccg aagcataagc cgccatcaaa gccccttgca cgccacttcg
       61 cgccgcccga tttcctcttt caacggcacg gcgttcaaca tccgcagacg ttcggcaaaa
      121 tccgcaaacg gcagcagctc cgaaccgaaa cggcgcatat gccccgtatc ctgctggcag
      181 tctatcagtt ccacacccaa agacgcgaga aacggcacgg cgcaggcaaa cgcgattttc
      241 gacgcatccg gttgtaatgc gaacatcgat tcgccgtaaa acaccctgcc gatctgaacg
      301 ccgtaaaacc cgcccgccaa cctcgtttta ccgttttcat cgggataatg gcactcaaac
      361 gaatgcgcgt atcccatttc gtgcagcttc aaatatgccg tctgaaactc gggcgcaatc
      421 caagttccgt cctgattcgg gcgcaccgct gccgcacaat gcgcgaccac ttccgcaaaa
      481 cagccgttga ccgtaacccg atagctgcag ttgcgtagcg ttttctccag cgagcgcgga
      541 acgtgcagac gttcgggaac aatcaccgca cgcggcccga ccgcatacca aaaaaaccaa
      601 ccgtctcgca aaaaccaagg aaacacgccg ttccgatacg cctcgagcag ccgccccgcg
      661 tccaaatccc tgctcacgcc gaccagcccg tcgcaccgtg ccaaagcata ggcaggatcg
      721 ggaaaggcat aattgtcagg ggcaagcagc ggaatacgca tggcagttct ccaaaaacac
      781 agacggcatc caaaatcatg aatgccgtct gaacaacaaa ccgtccggat ttaacgtttg
      841 cccttggcct gacagtcgct gcacacgccg tacatataaa gcgcgtgatc gacgatgcgg
      901 tagccgtttt cctcggcgat tttgtcttgc agggcttcga tttcgggatt gtggaattcc
      961 gttacctcgc cgcacttcac acagacgata tggtcgtggt ggtcgccctt gtccaactca
     1021 taaaccgcct tgcccgtttc aaaatggtgg cgttgcaaaa tgcccgcctg ctcaaattgc
     1081 gtcagcacgc gataaatcgt cgccacaccg atttccacac cctcttccaa caaaatacgg
     1141 tacacatcct cggcactcaa atgctcttcc gcatgagtct cgaacaaatc caaaatcttc
     1201 aaacgcgggc cggtaacttt cagtccactg tctttcagtt gcgcaatgtt gctgaatttt
     1261 tccataatat tcaatatccc tgtaaaataa tagacgctat aatacgcaat ttcagcctgc
     1321 ttgcccacta tcacaccata gcagtttgca atagcaaaac cgcaacggcg gacgggcagt
     1381 cggacacaga cagggtgaaa taccgcttaa gcgtatgatt atcgttcgct tttataaaat
     1441 attcaagcag tgctacacta gacatcccga tttgcacaga aaggttctcc cgtgaacaaa
     1501 accctcatac tcgtcctttc cgccctgttc agcctgaccg cgtgcagcgt cgaacgcgtt
     1561 tcactgttcc cctcgtacaa actcaaaatc atacagggca acgaactcga accccgcgcc
     1621 gttgccgccc tgcgccccgg catgaccaaa gaccaagtcc tgctcctgct cggcagcccc
     1681 atgctgcgcg acgcattcca taccgaccgc tgggactata ccttcaacac ctcccgcaac
     1741 ggcatcatca aagaacgcag caatctgacc gtctattttg aaaacggcgt gctcgtacgc
     1801 gccgaaggcg acgccctcca aaatgccgcc gaagccctcc gcgcgaaaca aaacgcagac
     1861 aaacaataag gaacacacat gataccgctc aaaatcgcca tcgccggcgt caacggccgc
     1921 atggggcgcg tattggttga agccgtcaac aaccatcccg acaccgtcct ttccggcgcg
     1981 ctcgaacact cgggctcaga agccctcggg ctggacgcag gctacgccgt cggactcaaa
     2041 accggcatcg ccatttcaga cgacgttgac accgttctcg cacaaagcga cgtactcatc
     2101 gactttaccc gccccgagcc gaccctcaaa cacctgcaaa aatgcgttga aaaacaagtc
     2161 aacatcatca tcggcacaac aggcttcgac gatgcgggca aagccgccat ccaagccgca
     2221 ggcgaaaaaa caggcgttgt cttcgccgcc aacttcagcg tcggcgtcaa cctcaccttc
     2281 cacctcctcg acaccgtcgc ccgcgtatta aacgaaggct acgacatcga aatcattgaa
     2341 ggtcaccacc gccacaaagt cgatgccccc agcggcaccg cgttacgcat gggtgaagtc
     2401 atcgccggcg cgctcggccg cgacctcaaa caatgcgccg tttacggccg tgaaggccac
     2461 accggtccgc gcgatccgtc caccatcggc tttgccaccg tccgcgcagg cgacatcgtc
     2521 ggcgaccaca ccgccctctt cgccaccgac ggcgaacgcg tggaaatcac ccacaaagcc
     2581 agcagccgca tgacctttgc cgccggtgcc gtccgcgccg cagtttgggt aaacggcaaa
     2641 acgggtttgt acgatatgca ggatgtactc gggctgaaca accgttaacc cccatacaaa
     2701 atgccgtctg aaaagatatt gttcagacgg cattttgccg acaggctccg tatcggcata
     2761 tcaatgtttc aacacacagg acgacacata aagcgtcgcc ctatgtgttg ccctgattcg
     2821 gaaggggtta cgcccctccc aaataaagtc tgattctacc gccccgaagg acagatgtcc
     2881 gagtggcggg gtttcaaccg aaaaggaaat acgataaagt tgcctgctca gcataacaag
     2941 ctacggctcg tttaattgaa actctcctga tctaaaaatt ctaactctat ttcccggcaa
     3001 actatatcta tactaaaacc ggtcggaaga tcatacctac tgcctatcac ttccagcgca
     3061 cctccataat ccaagatttc atcatcctgc tttaattctc caagttccaa tacatttttg
     3121 atatttttat cttggttctt cgtggcgtat ccatagataa atttcagata gctgaactgc
     3181 ttgaatttaa tttttaccca gtctgtataa tttggtgtag gaatctcttg aggaaagtcc
     3241 ggataatcca cattctggac gatgatatgg aattcatcgt ttttcaaagt atagtttaat
     3301 agagtgccct catatatata cagaaattga taatcttttt ttggaatggt ataagttgtc
     3361 atattatttt accttttcaa aaacaatgag tttaagattt tttgagtcca agttggttct
     3421 actactctta tgaaatttca gttgtttaaa ctcttccttt attttaagga atttttcaac
     3481 tagccgagaa caaactggag caaaatggac gattgagttt aggaatcaac ctgattcagt
     3541 acatgggcaa agattagaat taaaatttag ataaaggtta cgttatgaat gtgaagataa
     3601 aatataatct tttagaatat tgtgaatatt taggtggtat ttttggtgtt tataaaaatt
     3661 atttaaatat ggatatcaaa gatccaaatt taaaaattag attttttgat tttttggaag
     3721 aacttttatc agatggtgtt attgaattat gcgattatcg tgaaaatcct ccaaaaatat
     3781 taactggttc gccaaaaaca caggtagatg aattaagaag aatttggccg gatatggaag
     3841 agatgttgct atattttcca gataatcctt ggttttatgt agaacatttt tggtggggag
     3901 caacttgccc tattgaactg acacaattgc ctaaaataga aatttacgaa gaacaaatga
     3961 aacaaggtaa gtaagcgtat tagtataagc tataatcata aatcatatat aacaaaatta
     4021 atcagttaac tgaccattat attttaacct taatcccatt cgggccagtt ctgtttcatt
     4081 tggaattcct cttaaatact tattgtttga tgtgccatta atcacaattt ctcccccttt
     4141 ttttgtaata cgtgctgctt cgggaaaata atccattaac cgctcaacat cccgcctctt
     4201 ccaaaaccgc gcgcgcggcc aaatccgccg tatctttttt caacagcagg tgcaacgccc
     4261 tgaaatcctg ttgcagcgcg gcaaccttat cggggtgttc gtaccagtcc gccaacgccg
     4321 ccgccagttt ttccggtttt gcttcagatt gcaataattc cggcacagcc tccttaccca
     4381 acaggatatt cggcaggccg acatgcggca ctttgatttt gcgtttcaca taagcatagg
     4441 tcagcggcga aatcttgtag ctgatgacca tcggacgctt acacaacgcc acctccaaag
     4501 ttgccgtacc gctcgttacc agcaccgcat ccgccgccct gcacactgtt tcagactgtc
     4561 tgtcgattac cgtcagcggc aatccggcaa actccggccg ctgcaaaact tccgccaaac
     4621 gccgcttcgt cgcctccgtt gcggcaggca gcaggaagcg tgcggcggga tagcgttcca
     4681 acaacaataa tgccgtctga aaaaacaccg gcgccatata gtcgatttcg ctgacgcggc
     4741 tgccgggcag cagggcgaat acggggatgc cggcatccac gcccaaagtt ttccgcgccg
     4801 tttcacggtc gtcttccaag ggcataagct gcgccatcgg atgaccgaca aactccgcac
     4861 gtccgcccgc atcaagataa agctgcggct ccatcgggaa caggcacagc acgcggttga
     4921 cctgatgcac gattttgccc acgcgttccc gccgccacgc ccaaaccgac ggactgacat
     4981 aatgcacggt cggaatcccc gaccgtttga gccgctccgc cacacccaga ttaaaatcgg
     5041 gcgcatcgat gccgacaaag acatcaggtt tcaacgacag caaatcccgt accagccccc
     5101 tgcgtatccg taaaatttcc ggcagccgcc tgaccacttc ggcaaagccg cgcaccgcca
     5161 gtcgctcctg atcataaagg ctctcgaaac cttccgcctt catcagttcg ccgccgatac
     5221 cgacaaaacg cgcctgcggg caacgcttgc ggatggcgcg tatcaggtgc gcccccaaca
     5281 ggtcgcccga cgcttcgccg acactgacag caatcaaagg gcttttttta tcaaccatat
     5341 tcgttcttct tcacatactt tcacgtcccg atgccgtctg aaggcttcag acggcatcgg
     5401 ggttcacatt ttcgtcccgt tttccaacat caccgcaaac tgtgccaaat ccagcggcag
     5461 ctccgccttc aacaccagcg gctcgcccgt gagcggatgg ttcaagtgca gctcggacgc
     5521 gtgcaaaaac atccgcttca aacccaactt ctgcaaacga cggttcgcct gataatcgcc
     5581 gtagcgttcg tcgcccgcaa tcggacagcc ttgagattgc aggtggacgc ggatttggtg
     5641 cgtgcgcccc gttttcaacg tcgcccgcac caaagtcagg tgcgacagcc cgacaccgtg
     5701 caaaatgccg tctgaaaaac ggcttaacac acggaacacc gtatgcgccg actgcccgtc
     5761 cgcactgacg cgcaccatct tttcgccttg tgcgccggta tatttgaaca ggggcagttt
     5821 gacatggaaa ttgtcgtccg gcagtttgcc cacccccagc gcaaggtaga tttttttggg
     5881 gtggtcgtta cggatggctt cgtgaagttt gacgagcgcg ctgcgtttct tcgccaccat
     5941 caacaagccg ctcgtatcct tgtccaaacg atgaaccaac tccaaatact tcgcctccgg
     6001 acgggcgcgg cgcaactgtt cgataacgcc gaaactcacg ccgctgccgc cgtggacggc
     6061 aacgccggac ggtttgtcga tgaccaaaag cgcatcgtct tcgtaaacaa cgtcaaacgc
     6121 acgcgccggt acggcggcac gcctttcaga cggcatttcc ttctccgcca cgcgcacagg
     6181 cggaatccgc accgtatccc cctccgcaat acggctgtcg ggtttgcagc gtttcttgtt
     6241 caaccgcacc tcgccggcgc ggataatgcg gtggatatgg ctcttggaca cgcccttgag
     6301 gatttttatc agatagttat caaggcgttg ccccgcctca tgttcggcga cccctatcaa
     6361 gctgaccgaa tctttgctta tttcgtgcgt tttcatctat aatccgaaca tccgtttcag
     6421 caaaaagcgc gcccgccgcg cctcctgaaa cggtttactt taaaacggaa ttttatatta
     6481 aaaacgcact ccgcaaagca ttttccctgc gcctgttccc agccggcagg cgcagaacgt
     6541 agtcaagttt gaattgattt tgccgtttcg gcgcggaagt aagacggcag ccgggcccaa
     6601 gccccaaacg caaaccgccc gaaaacacag gcatcacgat aacaagaaga agccggcacc
     6661 catttttccg accataccgc tcggaacgcg gcagacagac cgccgcatcg atctttatag
     6721 ccttctgtat ccgacccttc cgcacacggt tctccggaac gtgcatccct gcggattttc
     6781 aactcaaaaa ctgattaccg gttttatccg aagacatcgg aaaacccagc cttacggaat
     6841 gccgaaccgg accggcgcgc ccgaatgccg cccgtccacg aggtgatcat gaaaagaatg
     6901 ttattcaacg caacgcaggc cgaagagctg cgcgttgcca tcgtcgatgg ccaaaacctt
     6961 ttggacttgg acatcgaaac gctgggcaaa gaacagcgta aaggcaatat ctacaaaggt
     7021 atcattaccc gcatcgagcc gtcgctggaa gcatgtttcg tcgattacgg aaccgaccgc
     7081 cacggctttt tgccgtttaa ggaagtatcg cgttcatatt tccgcgacta cgaaggcggc
     7141 agggcgcgta ttcaggacgt gctcaaagag ggcatggaag tcattgtcca agtcgaaaaa
     7201 gacgaacgcg gcaacaaagg cgcggcgctg accactttca tcagcctggc cggccgctat
     7261 ttggtattga tgccgaacaa cccgcgcggc ggcggcgtat cccgccgtat cgaaggcgaa
     7321 gagcgtcaag agttgaaagc cgccatggcc gaactcgaca ttccaaacgg catgagcatc
     7381 attgcccgta ctgccggcat cggccgcagt gcggaagagt tggaatggga cttgaactac
     7441 ctcaaacaac tctggcaagc cattgaagaa gcaggcaaag cacaccatga cccttacctg
     7501 ctctttatgg aaagctcact gctgatccgt gcgattcgag actatttccg tcccgatatc
     7561 ggtgagattt tggtggacaa tcaagaagtt cacgaccaag ttgccgagtt catgagctac
     7621 gtcatgccgg gcaatgtagg ccgtctgaaa ctctatcaag accacacgcc gctgttctcc
     7681 cgtttccaaa tcgaacacca aattgaaagc gcgttctcac gcagcgtcag tctgccttcc
     7741 ggcggcgcga ttgtgatcga ccacaccgaa gccctggttt ccatcgacgt caactctgcc
     7801 cgcgccacac gcggctctga cattgaagac accgcgttca aaaccaatat ggaagccgcc
     7861 gaagaggttg cccgtcaaat gcgcctgcgc gacttgggtg gtttggtcgt catcgacttc
     7921 atcgacatgg aaaatccgaa acatcagcgc gatgttgaaa acgtcctgcg cgatgcgctc
     7981 aaaaaagacc gcgcccgcgt gcaaatgggc aaactttcgc gtttcggcct tttggaattg
     8041 agccgccaac gtctgaaacc ggctttgggc gaaagcagcc acgtcgcctg tccgcgctgc
     8101 gccggcacag gcgtcatccg cggcatcgaa tccaccgccc tgcacgtttt gcgtatggtt
     8161 caggaagaag cgatgaagga caacaccggc gaagtgcatg cacaagtgcc cgtcgatgtc
     8221 gccaccttcc tgctgaacga aaaacgcgcc gagctgtttg cgatggaaga gcgtttggat
     8281 gtcaacgtcg tcctgattcc aaacatccac ttggaaaatc cgcactacga aatcaaccgc
     8341 atccgcattg atgacgtaga agaagacggc gaaccgagct acaaacgcgt tgccgagccg
     8401 gaagaagacg aatccgccaa acctttcggc agcgaaaaag ccaaagcttc ccgtcctgag
     8461 ccagctgtca aaggcgtacg ccatacccag cctgcaccga ctgtcgcccc tgagaaaaaa
     8521 gcctcttggt gggacagctt caaagcttgg ttgaaacgca ttttcggcgg cagcgaaacc
     8581 caagccgcgc ccgctgccga aacctccgaa aaacgcagca cgacaaaccg cagcggcagc
     8641 cgcgccaaca accgccgcca aaacccgcgc cgcagcaaac gcgaaggcag caaagtagaa
     8701 gtccgcgaag tggcaggcaa aactgccgga caggaagcgc gcgccgacaa agccgaaacg
     8761 cgcaacaacg gcaaccgccg ccgcaacgaa cgcggcgacc gtgccgccgg acgcgccgac
     8821 gaagcggaaa tccaagaccg caacatacag cctgccgcaa ccgttgcaga tgccgcaccg
     8881 tccgaaaccg aagtgcaaac cggaaaacgc cgccgcaacg gcagccgcag cgaacgcggc
     8941 caaaccgcgc cggaaaccgc caccgttgcc gaaacaaccg ttcagacagc ggaaaacacg
     9001 ccgtccgaac cgcataccgc agaagacaaa ggcagcaagc ccaaatccga acgcaaccgc
     9061 cgcgaacgcg acagccgaga tgccaaagaa cgccgcgagc gcaacaatca gcgcgaccgc
     9121 cgtcaaaacg gcaaaaaacg caatattccg tctgccgcca aaatcgagca gtacctgaat
     9181 attcacgaca ccgccgacaa agtccgttcc gccgccgcgc acgttttcgg cgaaaccgac
     9241 gcaaacgcgc cgattaccgt cagcattgcc gatccggttg cagaaaggga tcttccgaca
     9301 gcatctcccg ccgtttcaaa cggcgacgca ccggtttatg atgcggcgga aaaaatccgc
     9361 cgtgccaccg ccgccatcct gcccgaaggc gcgacaccga aagccgaagc acaggaaatg
     9421 ccgtctgaaa ccgcaacctt tacggctgcg gcggaacagg cacgggaaac cgcacaaacc
     9481 ggcggactcg tcctgatcga aaccgaccct gccgcattga aggcatgggc ggcacaaccc
     9541 gaagtccaag ccggacgcgg tttgcgccgt tccgaacagc ccaagccgtc tgaagtcgca
     9601 accgtccctg ccgaagaaat gatccaagtc gaaacccggc aaggctgaac cgacggcggc
     9661 aaaaagaggt tctgttccgc agaacctctt ttttacatgg gttcggatac ctgcaatgcc
     9721 gtctgaaact tcgccattcc cgtgattacc gaaacattcc tccattccca tgattcccgc
     9781 aacattccgt cattcccatg attcccgcaa cattccgtca ttcccgtgaa aacgggaatc
     9841 tagaacctct aaactttcag ataatttttg aatattgccg ccgcccaaag gtctagattc
     9901 ccacaacctt
//
LOCUS       DAWFAD010000063         9874 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWFAD010000063 DAWFAD010000000
VERSION     DAWFAD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.063"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(312..545)
                     /locus_tag="WGO49_001170"
     CDS             complement(312..545)
                     /locus_tag="WGO49_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ0306281.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(711..911)
                     /locus_tag="WGO49_001171"
     CDS             complement(711..911)
                     /locus_tag="WGO49_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ0306282.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WGO49_001172"
     CDS             complement(1083..1958)
                     /gene="xerD"
                     /locus_tag="WGO49_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ0306283.1"
                     /translation="MEEGLIDRLLETLWLDRRLNQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVGRYYTEARPALLKGRSCDALFVSQKKTGISRQLA
                     WMIVKEYAGRAGIGHISPHSLRHAFATHLVQHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2010..2450)
                     /locus_tag="WGO49_001173"
     CDS             complement(2010..2450)
                     /locus_tag="WGO49_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ0306284.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            2771..3709
                     /locus_tag="WGO49_001174"
     CDS             2771..3709
                     /locus_tag="WGO49_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ0306285.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRLKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            3838..4131
                     /gene="hfq"
                     /locus_tag="WGO49_001175"
     CDS             3838..4131
                     /gene="hfq"
                     /locus_tag="WGO49_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ0306286.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPASTLVQVETVQQPAE"
     gene            4215..4781
                     /locus_tag="WGO49_001176"
     CDS             4215..4781
                     /locus_tag="WGO49_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0306287.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALNNTRCRLQEAGYSNVRFILDGHENLKQYIPKPLDAAIFNFGWLP
                     GGDKSLTTRTETSIAALSATLSLLKENGMLIAVLYPGHENGKQEAEAVGQWAKNLPQE
                     QFAVLRYGFTNRKNSPPYLLAFEKLRQK"
     gene            4835..5209
                     /locus_tag="WGO49_001177"
     CDS             4835..5209
                     /locus_tag="WGO49_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ0306288.1"
                     /translation="MQYLFVKYSHQIFVTITILVFNIRFFLLWKNPEKPLAGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            5219..5713
                     /gene="folK"
                     /locus_tag="WGO49_001178"
     CDS             5219..5713
                     /gene="folK"
                     /locus_tag="WGO49_001178"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ0306289.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLKQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDTRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDR"
     gene            complement(5838..6317)
                     /locus_tag="WGO49_001179"
     CDS             complement(5838..6317)
                     /locus_tag="WGO49_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ0306290.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYIALSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDCKDNAYKRALSALSRKL
                     GRCKDKLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAVFQI"
     gene            complement(6537..7274)
                     /gene="ubiE"
                     /locus_tag="WGO49_001180"
     CDS             complement(6537..7274)
                     /gene="ubiE"
                     /locus_tag="WGO49_001180"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ0306291.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7317..7685)
                     /locus_tag="WGO49_001181"
     CDS             complement(7317..7685)
                     /locus_tag="WGO49_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ0306292.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            7805..9424
                     /gene="mnmC"
                     /locus_tag="WGO49_001182"
     CDS             7805..9424
                     /gene="mnmC"
                     /locus_tag="WGO49_001182"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ0306293.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLREHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSPR
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMQQTYAKLALDKNYRIDAPCPYLPNAYANTAHGTRGLATAPICAAAVAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(9421..9570)
                     /locus_tag="WGO49_001183"
     CDS             complement(9421..9570)
                     /locus_tag="WGO49_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306294.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT         2626 a         3006 c         1989 g         2253 t
ORIGIN      
        1 taccgattac acaaacacat atcatcttta cacaatcatg cttccatcaa cagtaaaaca
       61 tgatatgatt gccaacaata acatctcaca ataaattttc taatttttat tgaaaaaatc
      121 aataaataag aattctccca accgacaaat aaaataaaga aagggttaat atgcaacatc
      181 gaagaagact agcaatttac caagcatcca aacgtgcttc ctttaccggc aggtcatccg
      241 ccccgcaaaa acgtaaagaa cgttgatttg aaaaaaatgc cgcctgaagt cctgcttcag
      301 acggcatttt tttaccgttc gagaaactgt ttcaacctgt cctcatccaa aaaccagtgg
      361 cagatttcct catctcccgc caacaaaacg ggaaccagct cattgtattt ttcttccaaa
      421 acaggatttt catccacatc gaccacttcc agcccgaacc cgtattcatt ttgaaaaggt
      481 ttgagtgcat cgcgcatttt gtggcacaag ctgcaatatt cacgaaacat caaggtcaat
      541 ttcatccgcg tttccttatc tgtcaatttg cacacgccaa agccttagac gcagcagaat
      601 catggtctat ttgggaaaaa acaatgtttt caaggaagat gatactcaag tcctgccaaa
      661 aacagcaaaa atgccgtctg aacagttcag acggcatttc caaaaccgtt ttacgcttga
      721 acgttgatac ccgccgtaac cgtcggttgc gcattgtgcg ccgtcagaaa cgaagcggca
      781 agctccccgc agcagccgca gcagctcgct acgcccaatt gcgactgcaa atcgcccatt
      841 gtggtcgcgc cggcggcgat tgtttccttg atttgatggt cggtaacggc attgcagatg
      901 cagacaaaca ttttgtgctc cgtgtgttct caaactatcg taccgatagc ggcttttatt
      961 atcgtatgca aatataaata aaaacggttc gcattgcaag gtcggcatac acggtttgtc
     1021 ttggtaattt tttatcaagt ttgcattttc gattgatttc ttattaaacc gaagcagaag
     1081 cctcagtttc gggaatggtg ttccttcact acaccctgca accatacatt ggcaacatgg
     1141 gtataaatct gcgtcgtatt caaatcggca tgtcccaaca tatcctgaac cacgcgcaaa
     1201 tccaatccgt gctgcaccaa gtgcgtggcg aaggcatggc gcaggctgtg cgggctgatg
     1261 tgcccgatgc ctgccctacc tgcatattct ttgacaatca tccatgccaa ctgacgggaa
     1321 atacctgttt ttttctgact gacaaacaag gcatcgcaac tcctgccctt caacaaggcc
     1381 ggacgcgcct ccgtgtaata gcgtcccacc caatacgccg attcctgccc catcgggacc
     1441 atcctctgct tgtcgccctt gcccagcgcg gtaatacagc ccctgtccaa atccacattg
     1501 ccgaagttca gcccgaccgc ctcgctgacg cgcaagccgg tcgcgtacat taattcaagc
     1561 agggccttgt cccgcaaacc gtgcggcgtg tcggtatccg gtgcggcaag cagtcgggaa
     1621 atctgctgtt ctgtaatcag ggtcggaata ttcttgtcga ttttgggcgg tttcagcaaa
     1681 cgggtgggat tgtccgtcct tatgccttca cgctccatcc atatatacag gcgtttgcat
     1741 gccgataatg cgcgcgcctg cgaactccgt tgctctccgt caacataaac cgccgccgcc
     1801 aaatccgctt cgtccgcatc cttcagcatt ctgcccgatt gggacaggcg gcgggcgatt
     1861 ttttccaaat cgcgccggta accgtttaaa gtattctgat tgagccgcct gtccaaccac
     1921 agcgtttcaa gcagcctgtc gatcaaacct tcttccatac cgttccaaac aaatgccgtc
     1981 tgaatcttct tcagacggca tggttcacat tatcgggaaa gcgtttccaa tacttcctgc
     2041 gcgtgacccg ccactttgac tttccgccat tcatgggcga tttctccatc cttattcaag
     2101 acgaacgtac tgcgctcgat acctaacgac tctttcccgt acagtttctt caatttgatg
     2161 acatcaaaca ggcggcacac tgtttcatcc ttgtcgctca acagctcgaa ccggaaaccc
     2221 tgcttggcgc aaaaattctg atgcgccttt acgccgtcgc gggaaatacc gaccacggta
     2281 taacccaatg cctcaaactg ttccaaacgc gcattgaaat ccaagccttc cgtcgtacag
     2341 cccggcgtac tgtctttcgg ataaaaatac acgaccaaag gcagatgttc tgccgaatga
     2401 aaatccgcac cgctgctcga aggcagggta aattcatatt tcacatccat agtcctactc
     2461 ccgatattcc cattattcaa aacggcacgc agaggcaccg ccgcaattgc caaaccaacc
     2521 ccgattctac cgccccaaag gacaaggatt caactgccgg aaacatccaa accgacacac
     2581 gacggcatga aaaatatcca tgtcaaacca caaaatatgt tccgatttaa aaacagaatg
     2641 ttataaaacc caatccccaa aacacaacaa gaccgcccgc ctacgggcag tctcctgtca
     2701 gacgacatac tttacagatg gctgtttttt caacaaaata acgccaatac tcaaaaatat
     2761 ggaatcaaaa atgtccatcc atactctgaa acgcctgccc tcatcgctgc tgctcggtct
     2821 ctgcctttcc ctgccgtcag cccacctttt tgccgacaac gacattttag ggcaattttt
     2881 agaacagaac atgcttacct cctccgatcc gatagaaata ttcgccgaaa gcacaataca
     2941 ccccaccaac acccaagcca ttacaggcgg tctgattctc tcctcacagt ctgccctggt
     3001 cgtcaacaac aaaaccggac agatactgta tcagaaaaac gccgacagga ttatgcccat
     3061 cgcctccatt tccaaactga tgagcgcgat ggtcgttttg gatgcaaact tggacatgaa
     3121 cgaaaccgtt accattacgc ccgacgaaat cgaccgcctc aaagggaccg gcagccgtct
     3181 tgccataggt acggcactta cacgcaaaga actgctgcac ctgagcctga tgagcagcga
     3241 aaaccgcgcc acccatgcat tgggcagaac ctaccccggc ggcatgggcg catttgtcgc
     3301 cgccatgaac cgtaaagccc aaagcctcgg tatgtacggc agccgctttt acgaaccgac
     3361 cggactcaac ttccaaaacg tttctaccgc caaagacctg agccttatgg tcaacgccgc
     3421 cgcccaatat ccgcaaatcc gcaccaactc gacttccaac tacgcctcgg tacagaccaa
     3481 aaacgggcag cagaactaca aaaactccaa tgccctggtc agagaaggca tgtggaacat
     3541 cgaattgcag aaaaccggct acatacgcga agcaggcagg tctatggttg tcaaagccaa
     3601 cattcaaaat caacccgtta ccatcgtatt gctgaactcg cccacatccg ccacacgcgt
     3661 caacgacgcc cgcaaaatcg aatcgtggat gctgcagcaa cgctcctgac atacaaatgc
     3721 ccggcggaaa accgcccaaa cacaaaaaaa attggtaatt tttcctattc aaggctacaa
     3781 ttcgacacgc acagggcgtc tgcccgattt cacaatcata ataacggagt taaaaatatg
     3841 acagctaaag gacaaatgtt gcaagatccc ttcctgaacg ccctgcgtaa agagcacgtt
     3901 ccggtttcga tttacctggt caacggcatc aaactgcaag gtcaggttga atctttcgac
     3961 caatacgtcg ttcttctgag aaacacttcc gtcacccaaa tggtttacaa acacgccatt
     4021 tccaccatcg taccggcacg ctccgtcaac ctacaacatg aaaacagacc ccaagccgca
     4081 ccggcttcga ccctcgtcca agtggaaacc gtccagcagc ctgccgaata atccgcacga
     4141 agcatgacgt gtcatatctt tcaatacctt accgacatcg gtaaggtatt tttattttca
     4201 gacagcattt aaaaatgtta ttgcaaaaca tccttccatt cgcccattgc cttttgcgga
     4261 aggcacttcc cgaaggcggc aatgcgctgg acggcaccgc cggcaacgga cacgacaccc
     4321 ttttcctcgc acaaaccgca ggcatccggg ggaaagtgtg ggcattcgac atccagccgc
     4381 aagccctgaa caacacccga tgccgtctgc aggaagcagg ttacagcaat gtacggttca
     4441 tcttggacgg acatgaaaac ctgaagcaat atattccaaa gccgctggat gcagccattt
     4501 tcaatttcgg ctggctgccc ggcggggaca aaagccttac cacccgcacg gaaaccagca
     4561 ttgccgccct ttctgccacc ttatccctac tgaaagaaaa cggtatgctt attgccgtcc
     4621 tctatccggg acacgaaaac ggcaaacagg aggcagaagc cgtcggacaa tgggcaaaaa
     4681 acctgcctca agaacagttt gccgttttac gttacggctt taccaaccgg aaaaacagcc
     4741 caccctatct gttggcattt gaaaaactgc gtcaaaaata actgtttgcg gtaaaataag
     4801 ccgattccaa cccttatttt gataagagcc gaccatgcag tatctgtttg tcaaatacag
     4861 ccaccaaatc ttcgttacca tcaccatttt ggtattcaac atccgttttt tcctactttg
     4921 gaaaaatcca gaaaagccct tggcgggctt ttggaaggca ctgccacacc ttaacgacac
     4981 catgctgctg tttacgggat tgtggctgat gaaaattacc catttctccc cgttcaacgc
     5041 gccttggctc ggtacaaaaa tcctgcttct gctcgcctat atcgcattgg gtatgatgat
     5101 gatgcgcgcc cgtccgcgtt cgaccaagtt ctacaccgtt tacctgctcg ccatgtgttg
     5161 cgtcgcctgc atcgtttacc ttgccaaaac caaagtcctg cctttctgaa acaccgttat
     5221 gaacaacaga cattttgccg tcatcgccct gggcagtaat cttgaaaacc ctgcccaaca
     5281 ggtacgcgcc gcattggaca cgctgtcgtc ccatcctgac atccgtctta aacaggcttc
     5341 ctcactgtat atgaccgcgc ccgtcggtta cgacaatcag cccgattttg tcaatgccgt
     5401 ctgcaccgtt tccaccactc tggacggcat tgccctgctt gccgaactca accgtatcga
     5461 ggctgatttc ggacgcgaac gcagcttccg caacgcgccg cgcacattgg atttggacat
     5521 tatcgacttt gacggcatct ccagcgacga cacccgactc accctgccgc atccgcgcgc
     5581 gcacgaacgc agtttcgtca tccgcccttt ggcagaaatc ctccctgatt ttgttttagg
     5641 aaaacacgga aaggttgccg aattgtcaaa acggctgggc aatcaaggta tccgtctttt
     5701 acccgacagg taattccgca cgcggatgcc gtctgaaagc ctttcagacg gcatttttcc
     5761 tttgccgcca acacgcgtgc aaaaaaatcg ccccttggaa aaggggcgca aaaggaacac
     5821 aaaccactac caaaacttta aatctgaaac actgcctgcc gcatactgta tccgacagga
     5881 tataaagccc tcactaaatc gtttcgagaa atccaaactt cttcatcgcc gacagaaaat
     5941 ctgcctttct ccggtaccag ctccaacaga aacggttgaa ccgccgtatg caacttgtcc
     6001 ttacaccgcc ccagctttct agacaaggcg gacagggcgc gtttgtaggc attatccttg
     6061 cagtcaagct cccgcgcact gccgacccag ctcaaacgaa ggttgcggct ttcatcctca
     6121 atcagcaaaa tgcctgtttt gacttccccc acctcgggct cgcatgaaag cgcaatataa
     6181 tatttgccgt cgatgttgac atacgccgct tcatgcacct gacagttgca gccggaagtg
     6241 gcaagcagcg aataaacaga gtcccgatgc agtttcaaaa tatcaaggga aatctgttta
     6301 aaaatacttt ccggcatttt tccctccttt cgtctgctgc gttgtttact ggattgactg
     6361 cattatacag acatctccca atcttgcaaa tgataattgt tcctattttt taaaaaattt
     6421 ataaattatt gatggatatg tgaattaatt ttaagattta aaatctcttt ctcaaaaaaa
     6481 cacaaaaatg ccgtctgaaa cgtgttcaga cggcattggc ttcacaggca gtccgtttaa
     6541 aatttcacgc ccttatgcag cgcgacgatg cccgcactca tattgtgata atccacgctg
     6601 tcgaagcccg catccagcat catctgtttc aaagtttcct gatcggggtg catacggatg
     6661 gattcggcga gatactggta gctgtccgca tctttggcaa tcagtttgcc catgaccggc
     6721 agcagcttga acgaatagaa atcatacgcg ccttcgagcg gcttgtagat tttggaaaac
     6781 tccaacacca gcaacgtgcc gccaggtttc aaaacacgat acatctcttt cagcgcggca
     6841 tctttatgcg tcatgttccg caagccgaac gccacggaaa ctaagttgaa ataattgtca
     6901 gggaaaggca gtttttccgc atcggcaagc gacacgggca aaatcatgcc ttcgttcaac
     6961 agacggtcgc gcccgacggt cagcatagag gaattaatat cggtcagcca aacctcgcct
     7021 tccttgccga cccgtttcgc ccaaccgcgc gacaaatcgc ccgtaccgcc cgcaatgtcc
     7081 aacactttat cgcctttttt caggtgcgcc gtgttgatgg tgaaatgctt ccacaccctg
     7141 tgcagccctg ccgacatgac atcattcata atgtcgtagt ttttggcgac ggagtggaac
     7201 acttccgcca ctttgccggc tttctcatct tcgttgaccg tgctgaaccc gaaatgcgtt
     7261 ttttgtccac ccatattcta accacctccc tgataccgcc ctacggcatc ccaaacttaa
     7321 caatctgatt gcttttcaaa acgcgacgcg cccgccgccg ccaatttgtc caaatattcc
     7381 tgccacatcg catcgtatcc gtatgccagt ctgtgcagat acgcccaatc gtaaagaccg
     7441 ctgtcgtgcc cgtccgaaaa actgattttc agcgcgtact gtccgacagg ctgcaaatcc
     7501 gtaatttgca catctgcctt accggtctgc aaaacatcct gtcccacacc gtgtccgcgc
     7561 acttccgcac tcggcgaata gacgcgtaaa aattcggcag gcagattttt aggttcgtcc
     7621 ccataagtca aaaccaacac ggtacggttt ttcaacaaac ggatttcaac aggaatatta
     7681 ttcaaaaaac acctcggttc ggcatacaat atccggcagg cgcaggcgtg ccttcaaacg
     7741 gcgcgtattt taacagcttt atcccgatac cgatattcaa acccacactt taaacggcat
     7801 tgatatggac aatctcgtat gggacggcgt tcccgacatc cgcacactcg accaaaccat
     7861 ccgcaaacac gcacacccgc tcaacctgat tgtctgcctc cccgataatc agattcccga
     7921 ttttcaaacc gcacaagatg cttcggacgc ggaatgccgt ctgaagcacc gtttggatca
     7981 ggcaacgcag tgcctccagt tcgacagcat caacctcatc gaacacatcc tgcccgatgt
     8041 ccgcttctgg ctggttcccc cttcacgcac ccgccgcctg cgcgaacact tccaccacat
     8101 ttcctggcag accgaagcca tcccgcaaac cgaaagtaag cccgacaaac cctggtttgc
     8161 acttccacaa acatccgaac ggcaaaaacc ggaacacatc ctcgttatcg gcgcgggcat
     8221 atccggcgcg gcaaccgccc acgccttagc atcgcacggc atttccgtta ccgtattgga
     8281 agcccgaaaa gccgcccaag ccgccagcgg caaccgccaa gggctgctct acgccaaaat
     8341 ctcgccgcac gacaccgaac agaccgaact tttgcttgcc ggctacggct acaccaaacg
     8401 cctgctcgga cacatcctgc ccgaatccga aacctggggc ggcaacggca tcatccacct
     8461 caattacagc cgcaccgaac aacaacgcaa tcacgaattg ggtttgcaaa aacaccataa
     8521 ccacctctac cgcagcatca cgcaggcaga agccgaaaaa atcgccggta tcccactgtc
     8581 cgtcccatac gaccaccctt catgcggact ctactggcaa cacggcgtat ggctcaatcc
     8641 acccgcattc gtccgcaccc tcctcaacca tccgctcatt ggactacacg aagacacacc
     8701 gttaaccgac atttcccacg acggggaaaa gtggattgca agcacgccaa acggcatatt
     8761 ttccgccaca cacatcatct actgcaccgg cgcgcacagc ccccgcctgc ccgaaaccaa
     8821 cctcgccgcc ctgcctctca ggcaaatacg cggacaaacc ggcctcacac cgtccacccc
     8881 gttttccgaa caactgcgtt gcgccgtttc aggcgaaagc tacatcagcc cgtcgtggca
     8941 cggactgcac tgctacggcg cgagttttat tcccaacagc agccataccg gatggaacga
     9001 agccgaagaa gcctcaaacc gccaagcatt ggcacacctt aaccccgccc tttccgaatc
     9061 attgtttgcc gccaacccaa acccccaaaa acaccaaggg cacgccgcca tacgctgcga
     9121 cagccccgac caccttcccc tagtcggcgc actcggcgac attgccgcta tgcaacaaac
     9181 ttacgccaaa ctcgcgctgg acaaaaacta tcgcatcgat gccccatgcc catatctgcc
     9241 taatgcctac gccaacaccg cccacggcac acgcgggctt gccaccgccc ccatctgcgc
     9301 cgccgccgtt gcagcccaaa tcctaggctt gccccatccc ttttcacaac gcctgcgcca
     9361 cgccctacac cccaaccgca ccatcatccg cgccatcgtc agaaggaagg atctaacccc
     9421 ttaagccttt gccgcatgcc gtcccgacca cagaaaatca tattcaaata aacataccaa
     9481 tcctgacggt aaaatatcga aaacacccat aacattttct tcccaaaata aggagtaagg
     9541 aatgcctaac aaattgcctt tgctgtccaa gatgaatgtt ccgtaacatt cgaagacttc
     9601 cttaaccctg ccagccgttt cggtttctgt accccctgtt agataaggaa ggaatgaaaa
     9661 agccctgttg attgtatgaa gtaaagggag ttaatttttg ttaatcttcc cttctcagga
     9721 actcaatata taaggatgtt aatttttatt tcccttctcg aggactcaat atagtggatt
     9781 aacaaaaacc agtacggcgt tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc
     9841 atcgccttgt cctgattttt gttaatccac tata
//
LOCUS       DAWFAD010000064         9807 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWFAD010000064 DAWFAD010000000
VERSION     DAWFAD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.064"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WGO49_001184"
     CDS             complement(128..1174)
                     /gene="adhP"
                     /locus_tag="WGO49_001184"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ0306295.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATHCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDEKLAFAKESGADLVINAA
                     KEDAAKVIQEKTGGAHVAIVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAESLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1457..1846)
                     /locus_tag="WGO49_001185"
     CDS             complement(1457..1846)
                     /locus_tag="WGO49_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0306296.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLSEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNKYFNVTLSKVSPDHFTLQADPNPTTND
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2072..3250
                     /locus_tag="WGO49_001186"
     CDS             2072..3250
                     /locus_tag="WGO49_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ0306297.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITEAVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATAKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3316..5250
                     /locus_tag="WGO49_001187"
     CDS             3316..5250
                     /locus_tag="WGO49_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ0306298.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAMSVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5553..6353)
                     /locus_tag="WGO49_001188"
     CDS             complement(5553..6353)
                     /locus_tag="WGO49_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ0306299.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAEPAVKAKS
                     AGKSVAASLKARLEKTYSAQDLKVLSVNETPIKGIYEVVVSGKQIIYTDAKGDYMLVG
                     DLINVNTRQSLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRL
                     EHEFEKMTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSI
                     CDNPVAETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6492..8681
                     /locus_tag="WGO49_001189"
     CDS             6492..8681
                     /locus_tag="WGO49_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ0306300.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSVRTAFVEEKPLPQSWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRPYHGQASHSEFVLNANQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SRMAAGRRTQDYLSAMSGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCSDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPVPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            complement(8728..9801)
                     /locus_tag="WGO49_001190"
     CDS             complement(8728..9801)
                     /locus_tag="WGO49_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0306301.1"
                     /translation="MRPFCVGATVLAILGALVFFISPGVVVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPATTLMAVLLLVAAVLLPFLPQLAAFFVAAYWLVLLLFCAW
                     LIWLARNTDNFALLMLLAAFTVFQTAYAATGDLNLLRAQVHLNMAAVMFVSIRVSVLL
                     GAEALKECRLKDPVFIPNIVYKNIAITFLLLHAAAELWLPAQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRTVLMNVNPIFFITVPAILTVA
                     VFVLYLFTFVPIFQANAFTGDPE"
BASE COUNT         2309 a         2522 c         2841 g         2135 t
ORIGIN      
        1 gacgtgttgc gatacgggtt ttcagacggt atttcaaaca gaacagatta aaatcaaatc
       61 caaatccata aaaaatgccg tctgaacagc gttcagacgg catttcgatg tgtatcgcca
      121 cgtcaaatca gtggtgatga ccgcagccgc attctttttt catatcgatc accatacggc
      181 cggtgatttt gccttcgcgc atttcttgga aaatggcggg tgcttcatcc aaagcacgca
      241 gttggacttt cggcacaacc aaactttccg cgccgaattg gaaggcttct tccaaatctt
      301 tgcgcgtgcc gaccaaagag ccgaccactt cgatgccgtc caaaaccaaa cgcgggatgg
      361 acaaatccat cgattccggc ggcagtccga tggcaaccac acgtccgccc gcgcggacgc
      421 aattcacggc agagttgaat gcggcggcgg atacggcggt aacaatggct acgtgtgcac
      481 cgccggtttt ttcctgaatc actttggcgg catcttcttt ggcagcgttg atgactaaat
      541 ctgcgccgct ttctttggca aaagccagtt tttcatcgtt gatgtcgatg gcgacgacgt
      601 gtgcgccaaa tacttttttc gcgtattgga cccccaagtt gcccaaaccg cccgcgccgt
      661 agatggcaat ccactgtccc ggacgaacac tggaaacttt aatggcttta taagtggtta
      721 caccggcaca agtaatgctc gaagcttgcg caggatccaa gccttctggg actttgaccg
      781 cgtaatcggc actcacgata cagtgggtcg ccatgccgcc gtcggcagta tagccagcgt
      841 tcaatacaga gcggcacagg gtttcgcggc cggtattgca gtattcgcaa gagccgcagc
      901 tttggaacag ccaggcgatg ctgacgcggt cgccgacttt cagatttttc acaccgtcgg
      961 caacttcttt aaccaaaccg atgccctcgt gtcccagcac gcggcccggt ttttcgccgt
     1021 agtcgcctgc cgcaacgtgc aggtcggtgt ggcacacgcc gcaatattcg acttcgacca
     1081 acgcctcgcc gtattccaac gggcgaacct cgcgttcgac tatttccaca tcgcccgcta
     1141 catttttatt cacaacaact gcctgcattt tcatgattgc gctccttagt tacggcaaaa
     1201 aaacctgtaa acggaatgtt gtccgatata agggtaagca tacgcttccg catctcacag
     1261 gtcaagtggt atgttgttga aaaatataga ttatatgtta tattataaca tcttggaaag
     1321 gcacggcatc ggggcggttg ccggatgagg ggcggcaggt ttcaagtttg aaaaaccgga
     1381 cggcaaaccc gtaaaaatgc cgtctgaagc tgtgtccgga cggcatcttt acgggtttgc
     1441 gggcttcggc ggaggattag tcgaagccgg ggcaggattg gtttgtaccg gaagcggcaa
     1501 tggtaccgcc gtcgttgagc gtaacgacgc aggtttcgcc gtcgttggtg gtgggattgg
     1561 ggtcggcctg aagggtaaag tggtcggggc tgactttgct taaggtaacg ttgaaatatt
     1621 tgttttgttt cagtttgttt ttgtcgtagg ttttaaacgt ccctttttgg cggtagtaac
     1681 gttccatggt ctgcgcgttg tgcagcaggg tcgtcctgac ttccgacagg cggacgcgcc
     1741 ggatgtaggt tttataggaa gggtaggtga tgagcgtcag gatgccgagg atggcgacgg
     1801 caatcatcag ctcgagcagc gtaaagcctt tttgaacgtt tttcatagca atgtgtttcc
     1861 atttgtttgt cggtcggcga cattataacg ccgtatcgga aatggcggaa tatgtaaacg
     1921 gattgaaatt ttcgggaaag cagattgtat aagccattta aaacaaatgg ttatttttat
     1981 tgtcggcagt ttgccgcctt ggatggggca gggacttgcg gtagaatccg cttccgattt
     2041 atgggattga cgcatacaga gaattgaaaa catggcaaaa atgatgaaat gggcggctgt
     2101 tgcggcggtc gcggcggcag cggtttgggg cggatggtct tatctgaagc ccgagccgca
     2161 ggctgcttat attacggaag cggtcaggcg cggcgacatc agccggacgg tttctgcaac
     2221 aggggagatt tcgccgtcca acctggtatc ggtcggcgcg caggcatcgg ggcagattaa
     2281 gaaactttat gtcaaactcg ggcaacaggt taaaaagggc gatttgattg cggaaatcaa
     2341 ttcgacctcg cagaccaata cgctcaatac ggaaaaatcc aagttggaaa cgtatcaggc
     2401 gaagctggtg tcggcacaga ttgcattggg cagcgcggag aagaaatata agcgtcaggc
     2461 ggcgttgtgg aaggatgatg cgaccgctaa agaagatttg gaaagcgcac aggatgcgct
     2521 tgccgccgcc aaagccaatg ttgccgagct gaaggctcta atcagacaga gcaaaatttc
     2581 catcaatacc gccgagtcgg aattgggcta cacgcgcatt accgcaacga tggacggcac
     2641 ggtggtggcg attctcgtgg aagaggggca gactgtgaac gcggcgcagt ctacgccgac
     2701 gattgtccaa ttggcgaatc tggatatgat gttgaacaaa atgcagattg ccgagggcga
     2761 tattaccaag gtgaaggcgg ggcaggatat ttcgtttacg attttgtccg aaccggatac
     2821 gccgattaag gcgaagctcg acagcgtcga ccccgggctg accacgatgt cgtcgggcgg
     2881 ttacaacagc agtacggata cggcttccaa tgcggtctac tattatgccc gttcgtttgt
     2941 gccgaatccg gacggcaaac tcgccacggg gatgacgacg cagaatacgg ttgaaatcga
     3001 cggcgtgaaa aatgtgctga ttattccgtc gctgaccgtg aaaaatcgcg gcggcaaggc
     3061 gtttgtgcgc gtgttgggtg cggacggcaa ggcggcggaa cgcgaaatcc ggaccggtat
     3121 gagagacagt atgaataccg aagtaaaaag cgggttgaaa gagggggaca aagtggtcat
     3181 ctccgaaata accgccgccg agcaacagga aagcggcgaa cgcgccctag gcggcccgcc
     3241 gcgccgataa acgaatatgc cgtctgaaca cggaaacggt ttcagacggc atttgttatt
     3301 gatttacgga atattatgag cttgatcgaa tgtaaaaaca tcaaccgcta tttcggcagc
     3361 ggcgagaacc gcgtccatat tttgaaagac atcagcctgt cgatagagaa gggcgatttt
     3421 gtcgccatca tcgggcagtc cggttcgggc aagtccacgc tgatgaacat actcggttgt
     3481 ttggataccg ccggttccgg ttcgtaccga atcgacggca tcgaaactgc caaaatgcag
     3541 cctgacgaac tggcggcatt gcgccgcgaa cgcttcggct ttatcttcca acgctacaac
     3601 ctcttaagct cgctgaccgc aagggataac gtcgcgctgc cagccgtcta tatgggcgcg
     3661 ggcggcaaag agcgttccgc gcgggcggac aaactcttgc aggatttggg tttggcaagc
     3721 aaagagggca acaagcccgg cgaactctcg ggcggacagc agcagcgcgt ctccatcgcc
     3781 cgcgccctga tgaacggcgg agaaatcatc ttcgccgacg agccgaccgg cgcactcgat
     3841 accgccagcg gcaaaaacgt gatggaaatc atccacaagc tgcacgaagc cgggcatacc
     3901 gtcattatgg tcacgcacga ccccggcatc gccgccaatg ccaaccgcgt catcgaaatc
     3961 cgggacggcg aaatcatttc cgacacctcg aaaaatcccg aaatccccgc aagcaatgtc
     4021 gggaggattc gggaaaaagc ctcgtggtcg ttttattacg accagtttgt cgaagccttc
     4081 agaatgtcgg tgcaagcagt attggcgcac aaaatgcgtt cgcttctgac gatgctcggc
     4141 atcatcatcg gtatcgcttc ggttgtctcc gtcgtcgcat tgggcaacgg ttcgcagaaa
     4201 aaaatccttg aagacatcag ttcgataggg acgaacacca tcagcatctt cccggggcgc
     4261 ggcttcggcg acaggcgcag cggcaggatt aaaaccctga ccatagacga cgcaaaaatc
     4321 atcgccaaac aaagctacgt tgcttccgcc acgcccatga cttcgagcgg cggcacgctg
     4381 acctaccgca ataccgacct gaccgcttct ttgtacggcg tgggcgaaca atatttcgac
     4441 gtgcgcggac tgaagctgga aacggggcgg ctgtttgacg aaaacgatgt gaaagaagac
     4501 gcgcaggtcg tcgtcatcga ccaaaatgtc aaagacaaac tctttgcgga ctcggatccg
     4561 ttgggtaaaa ccattttgtt caggaaacgc cccttgaccg tcatcggcgt gatgaaaaaa
     4621 gacgaaaacg ctttcggcaa ttccgacgtg ctgatgcttt ggtcgcccta tacgacggtg
     4681 atgcaccaaa tcacaggcga gagccacacc aactccatca ccgtcaaaat caaagacaat
     4741 gccaataccc aggttgccga aaaagggctg accgatctgc tcaaagcgcg gcacggcacg
     4801 gaagatttct tcatgaacaa cagcgacagc atcaggcaga tagtcgaaag caccaccggt
     4861 acgatgaagc tgctgatttc ctccatcgcc ctgatttcat tggtagtcgg cggcatcggc
     4921 gtgatgaaca tcatgctggt gtccgttacc gagcgcacca aagaaatcgg catacggatg
     4981 gcaatcggcg cgcggcgcgg caatattttg cagcagtttt tgattgaggc ggtgttaatc
     5041 tgcgtcatcg gcggtttggt cggcgtgggt ttgtccgccg ccgtcagcct cgtgttcaat
     5101 cattttgtaa ccgacttccc gatggacatt tccgccatgt ccgtcatcgg cgcggtcgcc
     5161 tgttcgaccg gaatcggcat cgcgttcggc tttatgcctg ccaataaagc agccaaactc
     5221 aatccgatag acgcattggc acaggattga ggtttgaaca aagatgccgt ttgaagctgc
     5281 cggaccggtc attttggagc agaaacttat tggacaaaaa cggtttctta gattctacgt
     5341 tccagattcc cacttgcgtg ggaatgacgg cggtgtgttt cgtattgttc agtttattat
     5401 tttcaatcat tcaatgggtt aggatgtatt tgttggcttg ctgtactggt ttaaatttaa
     5461 tccactataa atttcagtgc ggattggttt tcaggcggca tttcccgcaa aaaaggcttg
     5521 gaattttcca agccttttgt tttgcggatg gattattgat ttttgcggat gatttcctcc
     5581 agttggggca tcgggctgta gccgctttgg ctgcgcccgt tggggaagac gagggtcggc
     5641 gtgccgttga agccgaattg ttcgcccaag gaagtggttt ccgcgacggg attgtcgcag
     5701 atgctgccgc cgaccgggaa tttgccttta cgcatccaat ccgtccacgc tttggcgcgg
     5761 tcgggctgac accataagat ttgcgccttg cgcgcggcat cagggtgcag gccggcaatg
     5821 ggcatcataa agctgtaaac cgtcacgtcg gtcatttttt caaactcgtg ttccaagcgt
     5881 ttgcagaacg gacaatcggg gtcggagaag acggcgactt tcagcttgcc gttgccgcgc
     5941 acttctttga tggctttgtc caaaggcagg gaagcaaagt cgattttgtt caaatcggcg
     6001 gcgcgttctt cagtcaggct ttggcgcgtg ttgacgttga tgaggtcgcc aacaagcata
     6061 tagtcgcctt tggcatcggt atagatgatt tgtttgccgc tgacgacaac ttcgtaaatg
     6121 cctttaatcg gtgtttcatt gacgctcaac actttcaaat cttgggcgga ataggttttt
     6181 tccaaacgcg ctttcaaaga ggcggcaacg gatttgccgg cggacttggc tttgacggcg
     6241 ggttcggctt tgacggcggg ttcggcgttg gcattggaaa cgggcgtttg cccgcaagcc
     6301 agcagcggga ggacggtaaa gggggtcaag attttgatta acttggtttt catataaaga
     6361 tgattgcgcg tgttggaaag gcggaattgt atcaaatctc tgttgcgcct gcattgcgcc
     6421 taggctcaat ttatcgtctg aaaatagctt ccgcctgtta aaatacgcaa aaaaatgatt
     6481 tgcctgcctg tatgatctac caccgcatcg ccgtaaacgt gccgctttca gacggccttt
     6541 tgacttattc ccattccgat ccgcttcctc caggaacgcg ggtgcttgtg cctttccgca
     6601 ataaaaccgt ggtcgggatg gtgtgggaaa cggatattgc gccggatatg gatacggcgc
     6661 ggattttgag cgttcggacg gcctttgtgg aagaaaagcc gttgcctcaa agctggcgtg
     6721 atttgttggc atttacgtcg cgttattacc actatccgac cgggcaggcg gtgtttgccg
     6781 cgctgccgca gggtttgaag gaaacgcgcg cggtggaaat gccgcagccg ccgttgtttt
     6841 atgctttgaa cgaagcgggc agggcgcaaa cgccgccgcc ggctcggttc aacaaaaaag
     6901 cggctttgtg ggatgcgctg ctgtcgggcg gaatgacgat ggcggcgttg aagcaggtga
     6961 acgcgcaggc ggcgaaatta atcgaagatt gggcggaaca gggttggatt gaaacgacgg
     7021 aagcggcgaa acctgtattg aggccgtacc acgggcaggc ttcgcactct gaatttgtgt
     7081 tgaatgccaa ccagcagaaa gcttccgatg ccattcagac ggcattggga cgcttccagc
     7141 cgtttctgct gtacggcatc accggcagcg gcaagaccga ggtgtatttt gatgcgatgg
     7201 cgaaagtgtt ggcgcagggg cggcaggtgt tgtttctgtt gcccgaaatc aacctcacgc
     7261 cgcagctttt gaagcgggtg gaaaaccgtt ttgccgacgt accgaccgcc gtgttgcaca
     7321 gccggatggc ggcaggcagg cgcacgcagg attatctgag cgcgatgtcg gggcaggcga
     7381 agctggtcat cggcacgcgg ctggcggtgt tcacgccgat ggatgatgtc gggctgattg
     7441 tggtcgatga ggaacacgac ggctcgttca aacaggacaa cgaattgcgc tatcacgccc
     7501 gcgatttggc ggtgtggcgg gcgaagcaga gcggctgccc gatcatattg ggcagtgcca
     7561 cccccagctt ggagagttgg cacaaggcgc aaagcggcgc gtaccgcctg ctgcaactga
     7621 ccgaacgcgc ccataccgcc gcgcaactgc cgcaagtgga catcctcaac gtaggccgtc
     7681 tgaaactcga caacggtttc tcgccgcaag ccttgcagct tttgaaacag aattttgaag
     7741 cgggcggcat gtcgctggtg tacctcaacc gtcgcggttt cgcgccagcg ctgttttgca
     7801 gcgactgcgg ccataccttc ggctgcccga actgctccgc caaaatggtg ctgcaccaac
     7861 gtgcccgcca actgcgctgc caccactgcg accaccgcga acccgtcccg ttcaaatgcc
     7921 ccgactgcgg caaccaagat ctgaccgccg tcggacacgg cacgcagcgc gtcgaagaaa
     7981 ccctgcgcgc cttcctgccc aaggcagccg tcgtccgtgt tgacagggac agcaccgcgc
     8041 acaaaaacga ctgggcggat ttgtaccgcc gcattgccga caacgaaatc gacattctgg
     8101 tcggcacgca gatgctcgcc aaagggcatg atttcgcgcg gctcaacctt gttatcgtgt
     8161 tgaacgccga cggcagcctg tacagcgcgg actttcgcgc cccggaaagg ctgttcgccg
     8221 agctgatgca ggtgtccggc agggcggggc gcgccgacaa acccggcaag gtgttgatac
     8281 agacccaact gcccgaacat cccgtcttcg ccgccgtcaa agcgcaggat tatgccgtgt
     8341 tcgccgaaaa cgaattgaac gagcggcaaa tgttcgcgat gccgcccttc ggtttccaga
     8401 ccgccgtccg cgccgatgcg ccgcgtgttg ccgatgcgat ggaatttctc aacgccgcca
     8461 aagaaaccct cgccccgctg ttgcccgaaa gcgtttccca gtttggcgcg gctccgatgc
     8521 tgatggtgcg ccttgccgaa cgcgaacgcg cgcaaatctt cctcgaatca acatcccgac
     8581 aagatttgca ccgagccgtg agtttgtggg tgcaggtgtt gcagcaaaac agggacggca
     8641 aaatccgatg gtcggtggat gtcgatccgc aggaggcttg attattggca atccgatgcc
     8701 gtctgaaaac cgtttcagac ggcattttta ttccggatcg cccgtaaacg catttgcctg
     8761 aaagatcggt acgaacgtaa agagataaag cacaaacacg gcgacggtca gaatcgcggg
     8821 gacggtgatg aagaatatcg ggttcacgtt cattaaaaca gtgcgcgaaa cggcagcggc
     8881 gaaaaggatg gggacggcga tgcggcagag tttggggtag tcgagcttgg taaagccgct
     8941 gtgccacagt ccggcggtca gccacaccat catcacgccg cccatcatgc ctccgagggt
     9001 aatcaggtgc aggggcgcgg aggcaggtaa gttttgcagt ttcgccgcgc ctgtccacaa
     9061 atagcctgcg gcggcaaaga gctggagcag gtaataagtg cggacgtagt gtttgcgcag
     9121 gagttcgtga tggtgcagtt cgcgcagttt ggcaagcagg ataaagccga cggcgagcgc
     9181 ggtaaaaccg gcggtttgcg cgggcagcca aagttcggcg gcggcgtgca agagcaggaa
     9241 agtaatggcg atgtttttat aaacgatatt tggaataaaa acagggtctt tcagacggca
     9301 ttctttaagg gcttccgcgc ccagcaaaac actcacacgt atggaaacaa acatcaccgc
     9361 agccatattt aaatgcactt gcgcgcgcaa caggttcaag tcgccggttg ccgcgtatgc
     9421 cgtctgaaaa acggtgaacg cggcaagcag catcagcagg gcgaagttat ccgtgttgcg
     9481 ggccagccaa atcaaccaag cgcagaacag caataaaacc agccaataag ccgctacaaa
     9541 gaatgcggca agttgcggta aaaacggcaa taaaacagcc gcaacaagca acaacaccgc
     9601 catcaaagta gtggcaggtt tcaggttgcc cgaaaaaccc gtccagtcca acaaagccgc
     9661 agtcaggaaa ccaccatatg ctgcaggcag catgagttct aagaagattt ggcggtgcag
     9721 gacgacgaca ccggggctga tgaaaaacac caacgcgccg agtatggcaa gcactgtcgc
     9781 gccgacgcaa aacgggcgca tagcaac
//
LOCUS       DAWFAD010000065         9800 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWFAD010000065 DAWFAD010000000
VERSION     DAWFAD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9800
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.065"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(13..1308)
                     /locus_tag="WGO49_001191"
     CDS             complement(13..1308)
                     /locus_tag="WGO49_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0306302.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1434..2321)
                     /locus_tag="WGO49_001192"
     CDS             complement(1434..2321)
                     /locus_tag="WGO49_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0306303.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2321..3286)
                     /locus_tag="WGO49_001193"
     CDS             complement(2321..3286)
                     /locus_tag="WGO49_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0306304.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNITISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WGO49_001194"
     CDS             complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WGO49_001194"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0306305.1"
                     /translation="MTATTASSAKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4622..4729)
                     /locus_tag="WGO49_001195"
                     /pseudo
     CDS             complement(4622..4729)
                     /locus_tag="WGO49_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4808..5077)
                     /gene="rpsO"
                     /locus_tag="WGO49_001196"
     CDS             complement(4808..5077)
                     /gene="rpsO"
                     /locus_tag="WGO49_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ0306306.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5300..6235)
                     /gene="secF"
                     /locus_tag="WGO49_001197"
     CDS             complement(5300..6235)
                     /gene="secF"
                     /locus_tag="WGO49_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ0306307.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTKGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6239..8092)
                     /gene="secD"
                     /locus_tag="WGO49_001198"
     CDS             complement(6239..8092)
                     /gene="secD"
                     /locus_tag="WGO49_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ0306308.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRVGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGAVWKPEAETAAGKE"
     gene            complement(8304..8570)
                     /gene="yajC"
                     /locus_tag="WGO49_001199"
     CDS             complement(8304..8570)
                     /gene="yajC"
                     /locus_tag="WGO49_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ0306309.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8698..>9800
                     /locus_tag="WGO49_001200"
     CDS             8698..>9800
                     /locus_tag="WGO49_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ0306310.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKIFRL
                     DNDTAISTGSLSAAHFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAIAEYRLKRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2509 a         2585 c         2434 g         2272 t
ORIGIN      
        1 tgcctgcgac gcttaacggc tgtctttcca ctgataatat ttcgagccga ggaaagaacc
       61 gagtttgcgc aaaaacggtt gcaggataat attcggctgg cgcaaccgct caaacggctt
      121 cagacggcat tcgtccccta aaattgcttc ggcaaccgcc agacctgcaa tgcctgttat
      181 cgccatcccg tgtccggaat aaccttgtgc ataaaaaaca tttgcggcca aacgtccgaa
      241 atgcgggaca aggttggcgg taatgtcgca ctccccgccc cacgaatatt cgattttgac
      301 atcgtcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg tcatacgcgc
      361 aggattgtcg ataaactcgt tatccttacc gccgaaaagc agtctgccgt ccgcgctgag
      421 gcggtaataa tccaaaatat ggcggttgtc gcatactgcc atattgttac ggataagccc
      481 ttttgcgcgc gcccccaagg gttcggtcgc aataataaag gtgctaacgg caatcgcctt
      541 gcgttccaaa ggctggaata tcgggttcaa acctgcatag gtattgacgg catacaccag
      601 atttttgcat tcgacgctgc cttcgggggt gtaaaccagc caaccgtttt gataaggttc
      661 aatgcgtatc atgggagaat gctcaaaaat cttcgtacca gcttcggcag cggcgcgggc
      721 gatgcccaac gtgtaattga gcggatggag atgcccggac aagggatcga actgtgcgcc
      781 ttggtacata tcgctgtcaa gctgctgctt cagttcagtg ttatcccaga gttgataatg
      841 agttgcaccg taatattttt gggcgtgctc atgccattgt tgcaattctt cccaatgctg
      901 cgaacggatg gcaaccgtgg cataaccgcg ctgccaatcg caatcaatgg catgtttgcg
      961 gacgcgttcg tccaccagtt cgaccgcctg caaagactgt tgccaaaacc attgcgcttg
     1021 ctccaaaccg acctgttttt caatttcctc cataccgcag gcgtaatcgc tgataacctg
     1081 cccgccactc cgtcccgacg cgccgaaacc gatacgcgcg gcttccaaca caaccgtttc
     1141 atgtccctgc tccgccaagg gcaatgcagt gcacaaacca ctcaatccgc cgccgatgat
     1201 acaggtatcg gttttcagac ggcattgaag tttcggataa acagtatgag gattaaccga
     1261 actgaaataa taagaaggca gatattcttg aaaatcaggg cgaatcattg tgtttgcttt
     1321 atcgggtata ttttcggacg gaatgataca ggctgtcgag ccatatcgtc caaacagaaa
     1381 atcggttgaa gaaaacaggc tgacccagtc atgcggtcag cctgccttat taattaattt
     1441 gctttctcgg cagccaattt ttcttggcgg taggcttctg ccgcttctcg gtcacgcttg
     1501 gttgcctgcc tcatcatcca ataattgacg atgatgacca atgttccgat gatgccgatc
     1561 aggatggttg ccaaaacgtt catctgcgga tcaagaccca gcttgatttt ggagaaaatc
     1621 acctgcggca atgtggatga accggggccg gagaggaatg aggtaatcac caaatcatcc
     1681 aaagacaggg taatgccgag cagaaagcct gaagcgatgg caggggcaat caaaggcaaa
     1741 gtgatgacaa aaaagatttt cagcggacgc gcgcccaaat ccattgcggc ttcttcgagc
     1801 gattggtcaa gctcaaccag acgcgaacgg ataacaacgg taatgtatgc catgcacagc
     1861 gtcgtatgtc ctaagaaaat agtgaaaaag ccacgatcga agtagagatg ttgtaaccat
     1921 tcgctgccct gcaaaaatat ctgtacctga ataatcagca gcagcataga cagaccggta
     1981 atcacgtcgg gcatcaccat aggtgcggaa atcataccgg cgaacaaggt actgccgcga
     2041 aaacgtttaa tccgcgccat cgcatagcct gccagcgtgc ccaaaacgac ggcggcaagc
     2101 gaagacacaa cggcaatccg cagcgacagc caagcggctt ccaagatggt gtcgttttcc
     2161 agcaatgcgc cgtaccactt ggtcgaaaag ccgccccaaa cggttaccag cttggattcg
     2221 ttaaacgaat agatgaccaa aacaaccagc gggatataca gaaacgccag cgacagtgcc
     2281 aacatcagtt tcaagaacca agataatttg gatttctgca ttatttggct ccttcttcca
     2341 attcgcggtt ttcataatgc tgaaacaggg caatcggcac gaccagcagc gcgaccatca
     2401 cgacggcgac ggcggaagcc agcggccagt tgttttgatc gaagaacgcc tgccacaaga
     2461 ctttaccaat catcaggttt tccgaaccgc cgaccagctc gggaatgacg aactcgccga
     2521 cagcagggac gaaaaccagc atggagcctg caataatgcc ggttttcgac aaaggcaggg
     2581 taatcgtcaa gaacgatttg accggccccg cgcccaaatc ggaagccgct tcaagcaggc
     2641 ggttgtcgag tttcaccagt tgcgtgtata gcggcagaat cataaacggc agataggcgt
     2701 aaaccatcac caaattgagc gaaaaggcat tgtagaacaa atccaaaggc tcgctgataa
     2761 tacccatttt aatcaacagg ttgtttacaa taccgttatg cccgagcaga cccatccacg
     2821 catagacgcg caacaggaac gatgtccaaa agggcagcat aatggcaagc agcaaaccat
     2881 tgcggacaga aggattggca cgagaaatcg cataggcggt cggataaccg accaacaggc
     2941 aaatcactgt cgtcatcaat gcggttttga ttgaagacca gtaggtcatc agatagatat
     3001 tgctgttttc actgtcgccg aacgggttaa gggtatgcca aaaattttgg aaaatatcgg
     3061 catagttttg ataactgatg gtgatattca gacgacctaa atcttcgtcg atgctggtca
     3121 gcggcgtaaa cggcggaatg gcgatttctt gttcggcaaa gctgattttc agcacgatgg
     3181 cgaacggaat cagaaacagc accaaaagcc aaatatacgg tacggcaatc accacacgct
     3241 gccccgggcg gcggaacagt ttgtttttca gtttattaag gttcattgca ttccccttaa
     3301 atcaacggaa cagtggcgta ggctggtttt ccggccagct gatatacacg gtttcgtccc
     3361 aagttggcgg tgtaatgttg cgtacatacc agtaaggtgc aggaacttgg cttttgacga
     3421 cgcgcccgtt ggcgagtttg atatggtata tggcaaagct gcccaaatag gcgatttctt
     3481 ttaccgtgcc tttcgcccag ttgtagtcgc ccaaatattc gggtttttct ttatataaat
     3541 caatatcctc tggtcgaata ctaacccaaa ggtcctgctc gctcggacca cccaaaccgt
     3601 gatcgatgcg gacgtggttt tccaaacctt cgcattcgat aacggcatag tcggcatgat
     3661 cttcaatcac cacaccgtca aagatgttgg tttcgccgat aaactcggca gtgaagcggc
     3721 tgttgggata gtcgtacacg tcgctgggtg tgccgacttg ctgcaactga ccgtcagaca
     3781 taatggcgat gcgggtcgcc atcgtcatcg cctcttcttg gtcgtgcgta accataatac
     3841 aggttacgcc gacttgttcc agcgtattga ccaactcaag ctgggtttgt tggcgcagtt
     3901 ttttgtccaa tgcaccgagg ggctcatcca gcagtagaat tttcggacgt tttgccagac
     3961 tgcgtgccaa agcaatgcgc tgctgctgac caccagaaag ctggtgaggt ttgcgtttgg
     4021 caaatttggt catctgaacc aggcggagca tttcttcgac gcgcgcggcg atttcgcctt
     4081 taggcatttt gtcctgtttc agaccaaagg caatgttttg ttctacggtc atatgcggaa
     4141 aaagcgcgta actttggaac atcatattga tggggcggtc atagggtgca agtttggtaa
     4201 tatcctgacc atcaaggata attttcccct gattggggct ttccataccc gccagcatac
     4261 gcagcagcgt ggattttccg ctgccggaac tgcccaaaag ggcgaagatt tcgtgttgat
     4321 aaatgtccaa gtcgatgtta tcgacagcgt aattgtcacc aaactttttc accaagcctt
     4381 ggatttgcag ataaggtttg gctgaagacg cagtggttgc ggtcataatg gcaatactcc
     4441 aataaaaaaa cgagtaccgg caaaacggat tttcgaatgg gtgataaaaa gctgtttgat
     4501 tgctggcggg agtcgcaacg tctgatgccg tctgaaactc ttgtgaagcg cacgggcagc
     4561 atgaaatgga acaagattcc aaagaacttt atattatatt agtttatgcg gttttcgggc
     4621 aatatagtgg attaaattta aaccagtaca gcgttgcctt gccttgccgt actatttgta
     4681 ctgtctgcgg cttcgtcgcc ttgtcctgat ttaaatttaa tccactataa atatggattt
     4741 gagcttgtcg gaaagcaaca gaaaagaaaa caccgcccat ttttctgggc ggtgtcggaa
     4801 agcgtaatta tttacgcaga cccaagcggg taatcaacgc gcgatacgta tcgggctggg
     4861 tacggcgcaa gtaggccagc aggcggcggc gttggctgac cattttcaac aggccgcgac
     4921 ggctgtggtg gtctttgggg ttggctttga agtggggggt caggtcgttg atgcggaaag
     4981 tcaacagagc gacttgtact tcggaagagc cggtgtcgcc ttctttgcgt tggaaatctt
     5041 taacgatttg tgctttttgt tctacggtca gtgccataat gaaaactcca aaaatataag
     5101 aatccccata gggattcaga caagtttgcc aagcctgaag acaacggcaa actccctatg
     5161 cccaagatag gacaacgtgg cattatgaca caattcccgc atttctgcac aatattttaa
     5221 gaccgtggaa gtaaccgtta aaaatgccgt ctgaagcatt gtctgcttga gacggcaatg
     5281 ttcagacggc atatgcgctt caaaccacga tttcttcttt cttcttcggt tctttaccga
     5341 tattgtcgcg gctcaaaccg aacattagca gaagcgggct ggcaaccaat acggaagaat
     5401 aaatgccgaa cacgatgcca atggtcaacg ccatagaaaa gccgtgcaag gccgcaccgc
     5461 cgaacaccag catagaaaca accatcgcct cggtcgaacc gtgggtaatg atggtgcggc
     5521 tcatcgttgc ggtaatcgcg ttgtcgatga cttccggcac ggcatgtccg cgcatcgccg
     5581 gcttgcggaa gttttcacgg atacggtcga agacgacgac ggattcgttc acagaatagc
     5641 ccaatacggc aaggataccc gccaagacgg tcagcgaaaa ttcccattgg aagaaggcaa
     5701 agcagccgag aataatcacg atgtcgtgca tattggcgat aatggcagat acggcaaaac
     5761 gccattcaaa acgcatcgac aggtaaataa tgatgccgat aacgacaaaa cctaaagcca
     5821 tcaatccatt acttaccaat tcctcaccga cttgcgggcc gataaattcg acttggcgca
     5881 aggtaacgtc gggactgtct tttttcagca aatccataac ctgattggac aactgtgcgg
     5941 aagtaacacc ttctttgttc ggcaggcgga tcatgatgtg tttgttcgta cccaatgcct
     6001 gaacctgtac atcacctatt ttcagcgtat cgaggcgttc gcgcatctta ttgacatccg
     6061 caccctgctg atattggact tccattaccg taccgccggt aaattcgaca gagaaattca
     6121 ggcctttggt caccaagaag aacacggcag cgataaacgt aaccaacgaa atgaaggtcg
     6181 tcagtttgcc gtagctcata aacggaatat cgcgtttgat tttaaagagt tccatagctt
     6241 actccttacc tgccgcagtt tcggcttcag gtttccatac cgcaccgatg gaaatattct
     6301 gcaatttgcg tctgcgtccg taccacagat tgaccaacgc acggaatacg acgacggacg
     6361 aatacatcga agtcagaata cccaaacagt gtacgaccgc aaaaccgcgt accgggccgg
     6421 aaccgaatac caaaagcgcg ataccggcaa tcagcgaagt caggttggaa tcgacaatgg
     6481 tcgcccatgc gtgttggaaa ccgagattga ttgcctgctg cggcggcacg ccgacacgca
     6541 attcttcgcg gatacgttcg ttaatcaaga cgttggagtc gattgccata cccaaagtca
     6601 acgccagcgc ggccataccc ggtaacgtca acgttgcctg catagcagac aaaataccga
     6661 ttaggaacag tatgttggca ctcaatgcaa tggtagaaaa gaaacccatc agacgatagt
     6721 aaaccaccat gaatgcagca acgatggcaa aaccccataa agtcgaatgg aagccttttt
     6781 cgatgttctc cttacccaaa gacggaccga tggtacgttc ttcgacaatc tgcatcggcg
     6841 cggcaagaga accggcacgc aacagcaaag acgtatcatt ggcttcggct gtcgtcatgc
     6901 ttccggaaat ttccacgcgt ccgccggtaa tggcagtacg gataaccggc gcggttacaa
     6961 cctcggattt tccttggtcg atcaaaacca tcgccatgcg tttgccgaca tttgcggcag
     7021 tcagttcgcc gaaaatgctg ccgcccgcgc tgtccaagct cagactgacg gcaggtgcgc
     7081 ccatttggtc gaaactcggt tgcgcatcgt tgatgttgtc gcccgtcagc tcgacctgtt
     7141 tgctgattag cagagtttcg ggacgatctc cgccgcttga aagcagctca taaccgctcg
     7201 gcacgttgcc ttccaatgcc tcgcgcaact tggcaggatc gtcctccacc atacgcaatt
     7261 ccaaagtcgc ggtacggccg atgatgtctt ttgccttggc agtatcctga acgcccggaa
     7321 gctgcacgac gatacggtct gcacctgact gctggatgac gggttcggcc acgcccaact
     7381 cgttcacacg gttgtgcagg gtagtgatgt tctgtttgac cgcatcggaa cgcactttat
     7441 tgaccgcctc ttccgaaagc gtcaagacga tattgctgcc gtctgaattc agcgttgctt
     7501 caggaaacag cttgcgcaac tgcggcagtg ccttttgcac atcacctgca tcctgcaaag
     7561 ggacggtcag gctgtttcca gcctgacgca ccgtgccgct gcggattttt tcgcggcgca
     7621 gttcgcggcg gatgtcgccc gaataacgtt caaacgtttt ctgcatcgcc gctttcatat
     7681 cgacctgcat ggtgaaatgc acgccgccgc gcaagtccaa acccaaaaac atcggattgg
     7741 ctttgatttt cgccatccat tcggggctgt ccgccaacag gttgagcgcg gtaatatacc
     7801 cttcgcccaa agtgttttcg atgacgtcgc gcgctttaag ctgcgtttcg gtgtctttga
     7861 aacgcacttt cagtgaattg tccacaacaa acatcccgtc ggtctgaata cccgcgtttt
     7921 gcagcgcggc atccactttg gattgagtct gttcgttgat gatgatggct tgtcggttgg
     7981 tcgatacctg cacggcgggt gtttcgccga ataggttggg cagcgaatac actgcggcaa
     8041 ccgcaatcgt gaacacaatc agcagatatt tccataaagg ataacggttc atcattgttc
     8101 cttaatggtt ggaaccccac cctttcggtg gtgtcggaat cgggctattt cagaagaggc
     8161 aaaaaccctt cccagccagg caagaccgga aggcggcatc ttgaatatgc cgccctgcgt
     8221 gtcggaacat ggtcaagcct tcggttggaa ttcaaaacaa agtgccgcat tcgggctttc
     8281 cagatgcggc ttgtcggcac aaatcaatcg acttttgcgg caatcgcatt gcgttctact
     8341 tcgacctcga tttttgtacc ctgtccgata tccacggtaa aaaactgttc gccgactctg
     8401 gttaccttac ccttgaaacc tgccgccaag accactttgt cgccgacttt caaggcggca
     8461 agcattgcct gatgcgcttt gaatttcttt tgctgcggac gcatgatcag gaagtagaac
     8521 accaccataa tcaacactaa aggagcaaat tgtgcaacag cttgattcat aatttatccg
     8581 ttctttctaa tatggttgaa aatcgagagg ggtatataat aacataagac cgtaaacaat
     8641 atatcgggtt tgccgtccgt accgaccgta taccgcagcc tgcccgtcca caaacccatg
     8701 tccttgaccc gcctaatctt gaaattctat gcactgttgc gccttttttt gggcaaaaac
     8761 gcccgcaccg catggatttc gcatcccgcc tgtgccgggc acgaacccgg cgcaaaccat
     8821 cccgattcgc ccgaccgcat cctctgcatc gagcaggcat tgcgccgcgc cggtatttgg
     8881 cagcacctcc aaaccataga ggcggaagaa atcagcgata cgcgcctcgc acttgtccac
     8941 tcgagcaaat atctgaaccg tttggaatct tgcctgcccc aaaaaggcaa gattttccgc
     9001 ctggataacg acactgcaat cagcacaggc tcgctgtctg ccgcacactt tgccgccggt
     9061 tcggcagttc aggcagtcga catggtcatg aaccgtaaag catggcatgc cttttgcgcc
     9121 gcccgcccgc ccggacacca tgcgggcagc ggcaaagccg gcggattctg cctgctgaac
     9181 aacgttgccg ccggcgtcat gcatgccatt gccgaatacc gcctgaaacg cattgccgtc
     9241 atcgatttcg atgtccacta cggcgacggt acggcagaaa tattcaaaga cgatccgcgc
     9301 atcctgtttt tcaacctgtt tgaaaccgac cttttcccct tccccgaaaa caacgatatg
     9361 cccgacggcg gcaatatggt gcacctgccc ttgccgccgg gaacgggcag ccgcacattc
     9421 cgcgaagccg tccgcaggca gtggctgccc cggcttgccg cattcaaacc cgaactggtg
     9481 ctgctgtcgg caggattcga cgcacaccgt ctagacgaat cgggcaggct caacctgcac
     9541 gaggcggatt ttgcctggct gacacacaaa atcattcaga cggcatcggg ctgccccggc
     9601 aaaatcgtat ccgtactcga aggcggctac acccttgaac ctctggcaca atctgctgcc
     9661 gagcatatcc gcgtgctggc ggggctgggc aaatccgatg ccgcaaccgc ctatcagaaa
     9721 acactggatc caacaaaaaa acggtttgcc aaaccgaaaa ccggacaggt gcgccaacct
     9781 acccaatccg accgttacga
//
LOCUS       DAWFAD010000066         9678 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWFAD010000066 DAWFAD010000000
VERSION     DAWFAD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.066"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            66..260
                     /locus_tag="WGO49_001201"
     CDS             66..260
                     /locus_tag="WGO49_001201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306311.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
     gene            431..955
                     /gene="nspA"
                     /locus_tag="WGO49_001202"
     CDS             431..955
                     /gene="nspA"
                     /locus_tag="WGO49_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ0306312.1"
                     /translation="MKKALATLIALALPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            1091..1480
                     /locus_tag="WGO49_001203"
     CDS             1091..1480
                     /locus_tag="WGO49_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0306313.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            1558..2388
                     /locus_tag="WGO49_001204"
     CDS             1558..2388
                     /locus_tag="WGO49_001204"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ0306314.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            2403..3860
                     /locus_tag="WGO49_001205"
     CDS             2403..3860
                     /locus_tag="WGO49_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ0306315.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSCIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(3901..4290)
                     /locus_tag="WGO49_001206"
     CDS             complement(3901..4290)
                     /locus_tag="WGO49_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306316.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKGIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(4472..4873)
                     /locus_tag="WGO49_001207"
     CDS             complement(4472..4873)
                     /locus_tag="WGO49_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306317.1"
                     /translation="MKIKPLQFSNNSRRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFGF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            complement(5290..5619)
                     /locus_tag="WGO49_001208"
     CDS             complement(5290..5619)
                     /locus_tag="WGO49_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306318.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            complement(5645..6352)
                     /locus_tag="WGO49_001209"
     CDS             complement(5645..6352)
                     /locus_tag="WGO49_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0306319.1"
                     /translation="MVKTADGYKAIARIRTGDRVFAKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITF
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNDCPYDKGNQRYKDASYHGKNDNSV
                     KSRAPTNGQAALDNSVQVKSTSPRRVGVDKANNEIVVLDKTQTFNNGFAEYHGHVRSW
                     QDLHTDQKNALKKAGLD"
     gene            complement(6488..7033)
                     /locus_tag="WGO49_001210"
     CDS             complement(6488..7033)
                     /locus_tag="WGO49_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0306320.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFE
                     QFLLDNEVGNLSDMNRKPFTW"
     gene            complement(7020..7172)
                     /locus_tag="WGO49_001211"
     CDS             complement(7020..7172)
                     /locus_tag="WGO49_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306321.1"
                     /translation="MDAKSLDGKIKMPLYFSDGISVNKKVGIGAQNSLRLAGIGMKSL
                     RKNDYS"
     gene            complement(7154..7426)
                     /locus_tag="WGO49_001212"
     CDS             complement(7154..7426)
                     /locus_tag="WGO49_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306322.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPMGILPKPVALSIVQYEN
                     DPGFYLFYLDETGQEQTDTYHDTLDSAFEQAEFEFGISKEEWMQSP"
     gene            complement(7659..7982)
                     /locus_tag="WGO49_001213"
     CDS             complement(7659..7982)
                     /locus_tag="WGO49_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ0306323.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKSMIIEFFIEGSQKNLQSIES
                     QIISYRQDFQISIEQNEFGEMWTCYCSVNIIPSLENILAIETTLFDIAQKNDCNYEGF
                     GSYGN"
     gene            complement(7985..8692)
                     /locus_tag="WGO49_001214"
     CDS             complement(7985..8692)
                     /locus_tag="WGO49_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0306324.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDEASGKTGYKPVTARYGNPYR
                     ETVYIEISDGIGNNQTLISNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGDKAETEGVWVHNECPYGEKYRTEVGSYTNTHESGRT
                     YSGKGTRQRSQISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNN
                     YNRIESPGKKYRKQDGD"
     gene            complement(8826..9143)
                     /locus_tag="WGO49_001215"
     CDS             complement(8826..9143)
                     /locus_tag="WGO49_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306325.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEERRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(9146..>9678)
                     /gene="mafB"
                     /locus_tag="WGO49_001216"
     CDS             complement(9146..>9678)
                     /gene="mafB"
                     /locus_tag="WGO49_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0306326.1"
                     /translation="DQWMQENPNAAETVEALVNVLPFAKVKNLTKAAKPGKAAVSGDF
                     SDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGKHISGHRNYIEGKST
                     LNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKSGLSTNFGTIHSGKN
                     GVHIVPANPKTIKKVQ"
BASE COUNT         2466 a         2515 c         1948 g         2749 t
ORIGIN      
        1 cgcaaacaaa tcggcggccg cccgggaaaa cccgccccga acgcgtccgg aaaatatgct
       61 tatcgatgga aaacgcagcc gcatcccccg ccgggcgttt cagacggcac agccgccgcc
      121 ggaaatgtcc gacgcttaag gcacagacgc acacaaaacc gtatgcctgc acctgcaaca
      181 atccgacaga taccgctgtt ttttccaaac cgtttgcaag tttcacccat ccgccgcgtg
      241 atgccgccgc caccatttaa aggcaacgcg cgggttaacg gctttgccgt cggcaaagca
      301 gccggatacc gctacgtatc ttgaagtatt gaaaatatta cgatgcaaaa agaaaattta
      361 agtataatac agcaggattc tttaacggat tcttaacaat ttttctaact gaccataaag
      421 gaaccaaaat atgaaaaaag cacttgccac actgattgcc ctcgctctcc cggccgccgc
      481 actggcggaa ggcgcatccg gcttttacgt ccaagccgat gccgcacacg caaaagcctc
      541 aagctcttta ggttctgcca aaggcttcag cccgcgcatc tccgcaggct accgcatcaa
      601 cgacctccgc ttcgccgtcg attacacgcg ctacaaaaac tataaagccc catccaccga
      661 tttcaaactt tacagcatcg gcgcgtccgc catttacgac ttcgacaccc aatcgcccgt
      721 caaaccgtat ctcggcgcgc gcttgagcct caaccgcgcc tccgtcgact tgggcggcag
      781 cgacagcttc agccaaacct ccatcggcct cggcgtattg acgggcgtaa gctatgccgt
      841 taccccgaat gtcgatttgg atgccggcta ccgctacaac tacatcggca aagtcaacac
      901 tgtcaaaaac gtccgttccg gcgaactgtc cgtcggcgtg cgcgtcaaat tctgatatgc
      961 gccttattct gcaaaccgcc gagccttcgg cggttttgtt ttctgccacc gcaactacac
     1021 aagccggcgg ttttgtacga taatcccgaa tgctgcggct tctgccgccc tattttttga
     1081 ggaatccgaa atgtccaaaa ccatcatcca caccgacaaa gcccccgccg ccatcggcgc
     1141 gtacagccaa gccgtccgag caggcgacac cgtttacatg agcggtcaaa tccccctcga
     1201 tcccgccacg atgaccgtcg tcggcaacgg cgatttccgc gccgaagcgc gccaagtgtt
     1261 ccaaaacctg caagccgtcg ccgaagcggc aggcggcacg ctggccgaca tcgtcaaact
     1321 caacgcctac ctgaccgact tgggcaattt tgccgtcttc aacgaagtca tggcggaatt
     1381 tatcgccgag cctttccccg cccgcgccgc cgtcggcgtt gcctcgctgc ccaaaggcgt
     1441 gcaggtcgaa gccgaagccg tcctcgtttt gaacgcataa ttccacgttc ggactgcatg
     1501 atatttcaaa accatgccgt ccgaaccgca acactttccc acttcacaac atctgcaatg
     1561 gcacattacg caatcggcga catacaaggc tgtttcgacg aactgaccgc gctgctcggc
     1621 aaaatcggtt tcaaccacgg cacggacacc ctctggctga cgggcgacat cgtcaaccgc
     1681 ggcccgaaat ccctcgaaac gctgcaattc tgcatccggc acgaaaacag cgtgcaaatc
     1741 gtcctcggca accacgacct gcacctgctc gctgtcggct gcggcgaagg tgcgcccaaa
     1801 cgcagcgaca caatcgaacc catactcaaa caccccgacg gaaaaaaaat gctcgactgg
     1861 ctgcgtgcgc aaccgctgtt gatacgcgag ggcagccgcg tgatggtaca cgccggcatc
     1921 ctgccgcaat ggcgcataac caaagccgaa tcgctcgcta gagaagccga agccgaactg
     1981 cgcggcaaaa aatacgtcaa attcttctcc aaaatgtacg gcaacaaacc ggccgcgtgg
     2041 gacgaaggtt tgaaaggcta tgcccgcctg cgcttcatcg tcaacgcctt cacgcggatg
     2101 cgcgccctga cctttaaaaa cgaactggat ttcgactaca aatccacagt gaaaaaaatg
     2161 ccgccttacc tgcgcccgtg gttcaaagcc cccgaccggc aaaacctcga ccgcaccatc
     2221 atcttcggac actggtcctc gctgggctac acgaatgccg acaacgtcat ctcgctggac
     2281 accggcgcgc tgtggggcgg acagctgacc gccgtcaacc tcgaaacgga agaaattacc
     2341 caagtccaag ccgccggcgg catagactgg aaaagcttcg caaaataacg gaccgcccca
     2401 ctatgcacaa aatcctgccc atcgcccacg tcctctcccg actgggtatg ctgttttcct
     2461 ttatcctgct gatacccgcc gccctctcct acgccttttc ggacggcgcg tacaccgcct
     2521 tcgccaccac cgcgaccgtt accctttccg gctcgtgcat cgtccggctc gccaccctcc
     2581 ggttcaggcg cgaactgcgc ccgcgcgacg gctttaccct cgtcctgatg ttgtggctgg
     2641 cgtttgccgc tatggctgcg atgccgatgt acctgtattt cccgaatatg ggctttaccg
     2701 acgcattttt tgaatcgatg tcgggactga ccaccaccgg cgcgaccgtc atcccccacg
     2761 tcgacgggct cgccccctcc gtcaactttt ggcggcatat gctcaactgg ctgggcggga
     2821 tgggcatcat cgtccttgcc gtcgccatcc tgcctatgct cggcgtaggc ggcacgcagt
     2881 tattcaaagc cgaaatcccc ggcattgaca aagaaagcaa aatgtcgccg cgcatttccc
     2941 aagtggcgaa aaaactttgg ttcggctaca ccctgatcac catcctcgcg gcagcctgcc
     3001 tgcattttgc cgggatgggc tggttcgatg ccgtctgtca cgcgatggcg accctctcgc
     3061 tgggcgggtt ttccacccac gatgccagca tagcttatta caactccccc ctcatcgaag
     3121 cggtcatcat cgttttcacc attgtcggcg gcatcaattt tgccaaccat ttcgccgccc
     3181 tcaacagccg cagcctcaaa acctattgga aagacgaaga atgccggacg atgctgctgc
     3241 tgctctccgg cagcatcctt gccgccgccc tgtacctgtg gcacaccggc tattacgccg
     3301 gttttaccga atccctgcgc tacaccgcct tcaacttcgt ctccatcgga ctggcaaacg
     3361 ggctggccaa caccgacttc gcacaatggc cgctcctgat ttccctgtgg atgtttttcc
     3421 tcgccaacat cctcgccaac tccggctcga ccggcggcgg catcaaaacc atacgcgccc
     3481 tcgtcctgtt caaattcagc ctgcgcgaaa tgatggtgct gctgcacccc aaagccgtcc
     3541 gcaccgtcaa aatcagcggc aaggccatac ccgaccgcct cgcgctgacc gttatgtcct
     3601 tcatcttcat ctactttatg accgtcgtcc tcttcagctt cctgctgatg gcgagcggta
     3661 tggaatttac caccgccttc accgccgtca tcgcctgcat caccaacgcc ggccccggcc
     3721 tgggcgaagt cgggcccgcc ggcaattacg ccggtttgga cgtgatgcag aaatggatct
     3781 gcgttaccgc catgctcttg ggcaggctgg aaatcttcac cgtcttcatc ctcttcaccc
     3841 ccgcctactg gaagaaataa aaaatgccgt ctgaaaactt ttcagacggc atcttgtcct
     3901 ttaaagaagc ggaagctcta caaaaggaaa gaaatgctga aacctctgat aaatttcagg
     3961 atatttctct aaggctttta atgctttttc ttttgaaata ggcggatcat agacatctat
     4021 cccccttaag aaggcaatgc ccgttaatgc atttttttga tcatttgaaa tgtaaccata
     4081 attaacgact ccaattacat cataaatttc ccctataccc ttatcaaaat caatcaaatc
     4141 aaaaatatac aatggaattg tatcaatagg agtatctctg acaactatat ccaaccataa
     4201 tttaaattca gaaagattaa tagcatcata gaacatacag actattgcaa atcctaaatc
     4261 ttcacaattt tctttactaa ttttccacat aattatttac ctttatccat tgcttcccaa
     4321 ccgatacatc cggtatttta gcttctcctt tgccgtcaaa cactcctttg gttacctgct
     4381 ttgtgtcaaa aataccgtct gaaagccgaa tatcgtttca gacggcattt tgattgttgg
     4441 gtttttaatc agagctttgt ttgataattt gttattttga ataaaataca ggttctgtcg
     4501 agctgataac ttctattaca gaatcgctaa aaaagaaaat gtaagcctta taatatttct
     4561 tttcaaaaaa accaaaactt tcttctttca accagtccat ataactgtca tctattatct
     4621 cgaaaaaata atttatagca ggatttctat cccaatcatt tttttcttta ttttcaaata
     4681 aatattctga aaattttact ttataagatt catctgttat ttgaaaatga attatattgt
     4741 caataaatat ttcaatattt aattcataat ttcggtcgga tgccaaaatg ttcacatctt
     4801 gttcggtaac acaaattttg tctacgtaaa atctgcgact attgttagaa aattgtaatg
     4861 gtttaatctt cacaatcgtt ttccatatct aatttttgtt ttcttattgc cctgccagat
     4921 aaagcaggtc tgcctccgta ctgtctatcc tcacatttac tttcctgcca tcggctttta
     4981 tctccccggt tttaccgttt ttgccaactt tgcggctttg gcggcggcaa tgttgaaggc
     5041 ggcttcgacg gtttcggcgg cattggggtt gacccgtact catccgcaaa cggcaacacg
     5101 acggacggtg tttgcttttc agccgtctgc cgttttgacc aaggtgctgc cgtggaagga
     5161 gcaagtgtag gatttttgaa gaactcagtt ctcaatatcc tatttcaaac gaaggaaatg
     5221 ccgtctgaaa attaaacacg gtttcagacg gcattttgat tgttgggttt tcaacccgac
     5281 ctacggttcc taatgatttt cagataaaaa taaagaaata tcttctaacg catcttcagc
     5341 cctaccttga agctctggat catcttgctt tgaatgtagt aaagaaataa ctttatcgta
     5401 atcaccgata tttgaatgta gcctggcgag atgtccgaaa caggttagcg ataatccggc
     5461 aatatccaag tctttatttt tcgataattc catcaatttc tcagaaatcc acggtacttc
     5521 atatgctttg gaattaaata ccatatcaag aatgccatct ataacacttt gtttatcatc
     5581 agaagaaatc aacttttctg ttttactttt atcagtcatt ttttaatttt tccttttgaa
     5641 ttaactaatc caatcctgct ttttttaaag catttttctg atcggtatgc aaatcttgcc
     5701 aacttctgac atgcccgtga tattccgcaa aaccgttatt aaaagtttga gttttgtcta
     5761 atacaacgat ttcattattg gctttatcaa ccccaactct tcgaggagaa gttgatttaa
     5821 cttgaacgga attatcaaga gctgcttgtc cgtttgttgg tgctctactt ttcacagaat
     5881 tatcattttt gccatgataa gaagcgtctt tatatcgttg gttgccttta tcatacggac
     5941 aatcattatg aacccaaacc ccttccgttt ccgcctgatt gcccttgacg aagtaggtat
     6001 gccaatcggc gacggtcaga ttgtaggctt tgagcggctg ctgtttgaag gtaatgtttt
     6061 gaaccgtctg ttttgcgccg ctttcggaaa gcagggtgtc gccttttttc agacgacctg
     6121 cctgtatcca ttttccttga ctgtaaaacg ggtggatttt attggaaatc agggtttggt
     6181 tgttgccgat gccgtctgaa atttcaatgt aaacggtttc ttgatacgga ttgccgtatc
     6241 gggcggtaac gggtttgtat cccgtttttc cgcttgcctc gtccttggcg aagacgcggt
     6301 cgccggttcg gatacgggca atggctttgt agccgtctgc cgttttgacc aaggtgctgc
     6361 cgtggaagga gcaggtgtag gatttttgaa gaacttagtt cccaatatcc tgtttaaaat
     6421 aaggggaagg tcgcctgaaa attaaacacg gtttcagacg gcactttgat taaatttaag
     6481 aaaatcctta ccaagtaaac ggtttccgat tcatatccga aaggttgcca acttcattat
     6541 ccagcaagaa ctgctcaaaa gcattccaac cctttttttc caccaattct gcttcctgtt
     6601 tatacaacgg gacaagcaaa gggaaaacga tattgtagtg ttcgccataa cagacctgaa
     6661 aatcatcgtc gaaataaaat ggggcggaaa catacagtgc atccattttg ctaccttcga
     6721 taattaccct gggtaattga ataatttcac ctcttaaaat aggttgtcta ttgtcaatca
     6781 aatattcagc cactcctgat agaaaagctg caatattttc tttattccat tcttcagaac
     6841 aggtaaaaat taattcaaac ctactcttat agttcaagtc atatcgattc aagcctaaag
     6901 tagaaaaagt agttacccct ttatatggtt gtgatgggaa gacggaaacg ctgagatgat
     6961 aacgtcgatc caagcatttt ataccgcttt cgatttcacc caaataatgt tctaaatgtt
     7021 caactataat cattttttct taaagatttc atccctatcc cggcaagacg taggctgttt
     7081 tgggctccaa taccgacttt tttattgacg gaaatgccgt ctgaaaaata aagcggcatc
     7141 ttgattttgc cgtctaagga ctttgcatcc attcctcttt gctgattcca aattcaaatt
     7201 cagcctgttc aaatgccgaa tctaatgtgt catgataagt atccgtttgt tcctgaccgg
     7261 tctcatccaa ataaaacaaa taaaatcccg ggtcattttc atattgaaca atacttaaag
     7321 caacaggttt gggaagtatc cccatcgggg tacgatgaat cgttctaata ggaaaatctt
     7381 tattttcatc taagatgact ttgaataatt cttttacact catcattctt caatcccgaa
     7441 taattgttac cgcactattt tctattacac gtccattggc tttgtagccg tctgccgttt
     7501 tgaccaaggt gctgccgtgg aaggagcagg tgtaggattt ttaaagaact tagatctcaa
     7561 tatcctgttt taaatgaagg aaaagccatc tgaaaattaa acatggtttc agacgacctt
     7621 ttgattgttg tgtttttcaa cccaacctac acttcttgtt agttcccata cgaaccaaaa
     7681 ccttcataat tacaatcatt tttttgtgca atatcaaata acgtagtttc aatagctaaa
     7741 atgttttcaa gagaagggat aatattgacg gaacaatagc aagtccacat ttctccgaat
     7801 tcattttgct caatactgat ttgaaaatct tgccgataag aaatgatttg actttctatt
     7861 gattgcagat tcttttgaga accctctata aaaaactcta ttatcatcga ttttgtcaaa
     7921 tcaaacccta gttcttgata ttgtttcagt acatctgaag tagatttttc gtcaacaatc
     7981 atttttaatc tccatcttgt tttcgatatt ttttccccgg gctttcaatt cgattgtaat
     8041 tattggtact tttggaacca ccgtgagcgt caattcttct agattcatct ttgaatgcct
     8101 ctcgattatt tttggcagga gtaaaatctg tagctatatg agggtcatta ttaaccctag
     8161 cttctcttgc tccagagatt tgggaacgtt gtcttgtgcc ctttccagaa taagttcgac
     8221 cactttcatg agtattagta taagaaccaa cttctgtcct atatttttct ccatacggac
     8281 actcattatg aacccaaacc ccttccgttt ccgccttatc acccttgacg aagtaagtat
     8341 gccagtcggc aacggtcagg ttgtaggctt tgagcggctg ctgtttgagg gtaatgtttt
     8401 gaaccgtctg ttttgcaccg ctttcggaaa gcagggtgtc gccttttttc agacgacctg
     8461 cctgtatcca ttttccttga ctgtaaaacg ggtggatttt attggaaatc agggtttggt
     8521 tgttgccgat gccgtctgaa atttcaatgt aaacggtttc ccgatacgga ttgccgtatc
     8581 gggcggtaac gggtttgtat cccgtttttc cgcttgcctc gtccttggcg aagacgcggt
     8641 cgccggcttg aatatgggca atggctttgt agccgtctgc cgttttgacc aaggtgctgc
     8701 cgtggaagga gcaggtgtag gatttttgaa gaactcagtt ctcaatatcc tatttcaaat
     8761 gaaggaaatg ccgtctgaaa attaaacacg gtttcagaag acattgtgat tgttggtttt
     8821 ttaacctacg cttgcgaaat taattccttt ctcctgaata ctgcaccatt tatttggttt
     8881 aactccatag aagaatgatt agaataaata cattcaaagt cagcttcatc aaaatattct
     8941 ccacacatag accataactc tgtaatcaaa tattccatat cttcatggtc ttctgtgatg
     9001 gcttctcttg ataaataatt tttgattttt aaaaaccttc ttctttcctc gtaatctacg
     9061 gtaattaacc tgatatctga gtgtatcatg cccagcattg ctatattcat accgactcgc
     9121 agccagcttg gtaatatatt cataactatt gcaccttttt aatggtttta gggttagccg
     9181 gaacaatgtg aactccattt ttacctgaat gaatcgtacc aaaattggta cttaatcctg
     9241 atttcccatc gctgcctata ggatacccaa aatcaacaac aggatttcct cttgctcctt
     9301 tagaaataac tggataagca cctgaatgta ttccgttcaa caattcttga ggattaatgt
     9361 tttgatttaa agtactctta ccttcaatgt agtttctatg tcctgaaata tgtttccctt
     9421 gagctccatc atgaatttta gtcccaatag attttgcttt aaaatcaaca ttgcgggttt
     9481 gaaacatttc tccatctaca gactgagata atcttgaagc agtgttatgc ttgtaggagt
     9541 ctgagaaatc cccactaacc gcagccttcc ccggttttgc cgcctttgtc aggtttttga
     9601 ctttggcaaa cggcaggacg ttgaccaggg cttcgacggt ttcggcggca ttggggtttt
     9661 cctgcatcca ctggtcaa
//
LOCUS       DAWFAD010000067         9651 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWFAD010000067 DAWFAD010000000
VERSION     DAWFAD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.067"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(44..3076)
                     /locus_tag="WGO49_001217"
     CDS             complement(44..3076)
                     /locus_tag="WGO49_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEZ0306327.1"
                     /translation="MFWIVLIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNGAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDARRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDETADIHIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPSGFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSSGQYLSETEAFGHDSRAVCPFEDVPSERPSCRVSDTEADE
                     GAFQSEETDAVYEHLPTTDLLLPPLFNPGATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGGGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3241..3921
                     /locus_tag="WGO49_001218"
     CDS             3241..3921
                     /locus_tag="WGO49_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ0306328.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSAGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLACAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3950..4309
                     /locus_tag="WGO49_001219"
     CDS             3950..4309
                     /locus_tag="WGO49_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ0306329.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
     gene            complement(4442..4870)
                     /locus_tag="WGO49_001220"
     CDS             complement(4442..4870)
                     /locus_tag="WGO49_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306330.1"
                     /translation="MISIFKAYYDVYEFNGELLKRCIFLDEENEENNEIIKDRIYGYL
                     IYVLTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYNNEFDEE
                     DGWPDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            complement(5353..5889)
                     /locus_tag="WGO49_001221"
     CDS             complement(5353..5889)
                     /locus_tag="WGO49_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ0306331.1"
                     /translation="MDLREVKLGGETIYEGGFVSISKDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYVAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5923..6279)
                     /gene="folB"
                     /locus_tag="WGO49_001222"
     CDS             complement(5923..6279)
                     /gene="folB"
                     /locus_tag="WGO49_001222"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ0306332.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            6335..6937
                     /gene="plsY"
                     /locus_tag="WGO49_001223"
     CDS             6335..6937
                     /gene="plsY"
                     /locus_tag="WGO49_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ0306333.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAVLTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHLSWIWATLLIALLVLFRHKSNISNLLKGKEGKIGEKR"
     gene            7025..7870
                     /locus_tag="WGO49_001224"
     CDS             7025..7870
                     /locus_tag="WGO49_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ0306334.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPKLYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKELISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            8116..9090
                     /locus_tag="WGO49_001225"
     CDS             8116..9090
                     /locus_tag="WGO49_001225"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ0306335.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPSKPGKLRLMYEANPMA
                     LILEQAGASASNARQDILGIRPESLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(9249..9467)
                     /locus_tag="WGO49_001226"
     CDS             complement(9249..9467)
                     /locus_tag="WGO49_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ0306336.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         2272 a         2800 c         2189 g         2390 t
ORIGIN      
        1 ccgtctgaaa cagtcttcag acggcatttc catttgcaaa aaatcaagca ttgtccaagg
       61 ggacgagaat cgtacggttg ccgttgtgtt ccggtgcgga cacaatgcct tccgcctcca
      121 tctggtcaat cagacgcgcg gcgcggttgt agccgatacg caaggcgcgc tgtacgcccg
      181 aaatgctggc tttgcgcgtt ttcaggacaa cggatacggc ctcgtcgtac atcggatcgg
      241 tttcgtcgtc gccgctgcgc ccgatgccgg gcagctcttc gctgccgccg ccgctcaaaa
      301 tatcgtcaac atagtccggt tcgccaaact gtttcaaata ttcgaccacg cggtgcacct
      361 cttcatccga ggcaaatgcg ccgtgaacgc gctgcggata ggcagtaccc ggcggcagga
      421 acagcatatc gccctgaccg agcaggtttt ccgcgcccat ttggtcgaga atcgtgcggc
      481 tgtcgatttt gctggacact tggaacgcga tacgcgtcgg gatgttcgcc ttaatcagac
      541 ccgtgatgac atcgacgctg gggcgttgtg tggcaagaat caaatggatg cctgccgcgc
      601 gggctttttg ggcgaggcgg gcaatcagtt cctcgatttt cttgcctgcc gtcatcatca
      661 aatcggcaaa ctcatcgacc acgaccacga taaacggcag tttttccaaa ggttcgggat
      721 cgtcgggcgt gaggctgaac gggttgccga ttttctcccc ccttgctgcg gcttcggcga
      781 ttttttgatt gaaacccgca agattgcgca cgcccataaa gctcatcagg cggtagcgtt
      841 tttccatttc gttaacacac cagttcagcg cgtttgccgc cagcttcata tcggtaacga
      901 caggggcgag caggtgcggg atgccttcgt aaatgctcaa ttccagcatt ttcggatcga
      961 tcataatcat acgcacgtct tccggcgcgg ctttgaaaag catagacaga atcatcgcgt
     1021 tgacacccac cgatttgccc gaaccggtcg tgccggcaac caacaaatgc ggcgcttttc
     1081 ccaagtcggt tacgacgggc tgtccggtga tgtcctgacc gagcgcgagc gtcagcttgg
     1141 atttggattc ggcaaactcg ggtgaattga agatttcgct caggcgtatc atttggcgtt
     1201 tcgggttcgg aagttccaaa cccatacagg ttttgccggg gatggtttcg acaacgcgga
     1261 tggaagccac gccgagcgaa cgcgccaagt ctttttccag attcagaacg gaattgccgc
     1321 gcacgccgac atcgggttcg atttcataac gcgtaatcac ggggccggaa taagaatcga
     1381 caaccttgac cttgactttg aactccgcca atttttcttc gatggtgatg ctgttttcca
     1441 acagttcttc ttcggtttgc gtcgccccgg gattgaacag cggcggcaga agcaggtcgg
     1501 ttgtcggcag gtgttcgtat accgcatcgg tttcttccga ttggaacgcc ccttcatccg
     1561 cttccgtatc cgatacccgg caggacgggc gttcagacgg cacatcttca aacggacaaa
     1621 ccgcccgact gtcatgcccg aacgcttcgg tttccgacag atattgcccg cttgaccgct
     1681 ctgccgcacc ctcactgccg tcatccgcaa tagcggcggt ttcctcctgc caacctccat
     1741 tcaaaacatc atcggcaaga tggtcggttt cggcaatgcg gctgcgttgc gcctgctcga
     1801 atcctgacgg cggttcatag gtacggttgt agatttccga tacgggaggc ggcggcggaa
     1861 tatcgattgc gggcatggga actttcggca cttcgggtgg ttcgaccacc caagcatcgg
     1921 gcgcggcagg ttcttcaata tggatatcgg cagtttcatc ataaaccgga ccgccgtatc
     1981 ccacagacga aacggtttcc gtgaaaacat tttcggtttc agacggcatt tcgacatccc
     2041 gtttcccgac aaccgtccga ctttccggaa tcaggcgcga aatccgcgcc tccgcagaaa
     2101 ccgttgcctt gttcggttct ttcaaattga cggaaacgcg gcggcgggca tcgcggacgg
     2161 ctgtgccgtc tgaaacggac tgtccttggg aaacatccgg ggattttgcc tccgcctgcc
     2221 ctgcatgacg gcggaacggc gcattccgtg cattttcggc tttgactgca gaaaaggcag
     2281 acggatgctg cttctcaaaa tacggctcaa atccgtaatc cgcagactcg gaaaacgctt
     2341 ctttgtccgc atcgaacata cgggaatacg tgcgttgaag cacagggtcg tccaaaccga
     2401 taatctgcag cccttccatc ggggtatcgg acacgcggac tttggggacc gctgtttcgt
     2461 ttttctcaaa tgcatcgata tagcgttttt tcgtttccct taaagccgcg ccgtttaagg
     2521 cacgcgttgc ttcttccaaa gtgatttcct taaaaaccgg acggacgggc gaaatttcgc
     2581 tttcagacgg catcaaccct tcagcaatac tccggtcgaa tgggatgcgg cggttgtcga
     2641 ttacggcagt tgcaatgtct tccgtatcgg cagcctcttc ctccgcagct tctgctgctt
     2701 ccgtttccgc ttcttccgtc ccgtttccac tgtctgaata tccgtcggat gcgttcggaa
     2761 catccgcacg gtttgcagaa gcaggcggaa caggcctatc cggttcggat tcgtgcgcca
     2821 gatagtttcg gcagaaacgg acgacaccga cgaacagcca atacactgcc gttttgacgg
     2881 catggaaaag catcagggca agttcgggaa aatcgggcat accgtctttg atttcaggca
     2941 gctccgcctg tttttcccct ttcttttcct gccacgcaga aacctcgcgc atccactcgc
     3001 gttcggattg tgcgcggaca aaaaacaggc cggcaagcgc aagcaacaaa ataacgatca
     3061 aaactatcca aaacatatgc cgtttcccgc aagacaaacg attaaacggg tttattttaa
     3121 cgtgtttcca tcccaaaaaa aacaaccagc cggcaattct gccgaaaccg attcggcttt
     3181 cggtttgccc ccgccgacag tataatagcc gtcccttccc ttaatctttg cggcattgcc
     3241 atgattttcc aaacagtctg gttttcagat gtggtactgt cggtttcgtg gattgtcctt
     3301 atcctgatac tggcagcttc cgcgccgtcc gcattccgct cgcttgcccg ctaccgttcc
     3361 gcccttccac tgtgtaccgt aattttttcc gcagcatggt gtctgaacgc ctctgccggc
     3421 ggcggacagc ttgcccagat gaactaccat ctgctcgccg tcaatctggt tgcactgatg
     3481 gtgggcactt ccgccgcctt ttggcttgcc gcgctgctga tgctgcccta ctgcctgctg
     3541 tttgccggtt ctgccggcgc atatccgccg aacgctttgg tgctgattct gcccgcgctt
     3601 gccgtcaacc gcctgtcgcg tatgctggtc aaccgcctgc cgcccaatat tttcatcttt
     3661 attttcgtca acggctttct cgcttccgcc gccggcattc tgttgaccgg gctggtgctg
     3721 accggtattt tggatgctgc aaacgctttt ccgtccgaaa tattgtggac gaccgccctg
     3781 cccgtcttta ttctgctggc gtgtgcggaa gcttttctca gcggcatttc aactgccata
     3841 tttgttgcac tgaagcccca ttggatcaac actttcgacg acaaccgcta tctgaagtcc
     3901 gaccgcggca tatggcggta gccttcagac ggcatagaca ggcaaaacca tgctttccaa
     3961 catcatcccc ctttccatcg gtgcggcact cggtgcaacg gcgcgttggc tgctcaacct
     4021 cgccgttcct gccgccatgt ctcccgccac gggcaacctg tttgccaact ggacgggcgc
     4081 gctgctcatc ggcatctttg ccgaaaccat caatcacccg caatggaagc tgctgctgat
     4141 taccggtttt ctcggcagcc tgaccacgct ttccggattt tcactggaaa ccgtaaccct
     4201 gctccaatcg aatcgtcctg cttcagcact tgccaatatt ttcctacaca cggcaggttc
     4261 gctgctgctg acttggctcg ggctgaagat aggggcggca gtcaaataac cgcaacaaat
     4321 tgcttccttt ggttttgaca caaactatac aagtcctaac atcttaatca tggcgcagca
     4381 tataagcgtg gatttgcagc ccaatctata ctttctgatt ctattcatta agccattatg
     4441 cttattcctc aatcacggtt tccaccaccg aatgaatact tttaggcaat tgcaaaaaag
     4501 cattccaagc aattaaagca gttttaaaag tatgcagcga acagcttaaa tcaggccagc
     4561 cgtcttcttc atcaaattcg ttgttataga aaactacttt ttcagcagaa atatcccagc
     4621 aaaaaccttg tccacctcct gaataaactt cattgggatt attttctgct tgatggataa
     4681 attctaaatt ttcatctatg tctttttgta gataaaggtc tcctgttaaa acataaatta
     4741 agtatccata tattctatct ttaattattt cattattttc ttcattttct tcatctaaaa
     4801 atatacatct ttttaatagt tccccattaa attcataaac atcgtaataa gctttaaaaa
     4861 tagatatcat atcatttcct tatcttccat aaatcggctt cggttgttta aataccgccc
     4921 gtgccaatta ccgttctcaa attcaaattg ggcttttagg tctataacaa agaaaaagcc
     4981 ttggtaggat aaacctacca aggctttttc ttaaaaaaac gctctattac cgtgcctaaa
     5041 aatgcaaaaa agtaaccgtc ataacccttt tgccaaaatc aatatcatca tccgatgaat
     5101 gaattgacgg tatataagaa agaattaata agctcataaa ccggtaagaa aaatcagtaa
     5161 caaagatatt cattattaac cattcttaaa aatgatcatg ttcgtttggg taattatcat
     5221 acccttttcc ctcttattac taaagcacga atgactttaa aggacgcgcc cagagacaag
     5281 ccgaaaaggc atatcccatc cgccaaacaa aatgcccgcc gacttactcc ggcgggcagc
     5341 tccaacaccc tgtcaatcct tcatcagcca gtattgcaaa ccgattaatg tcttgccgtc
     5401 tttaatttca tcgtttgcca atgcctgacg gacttcctcc ttcgacatca atacggtttc
     5461 cgtaatctcg tcttcgtcat tggcaagcgt actgcccaag cgtacgcctt ccgcctcgaa
     5521 cagatacatt ttttcgttgc aaaaaccgac cgccgtataa aaactgtaca acaggcgtac
     5581 actgtcggca acataaggcg tttcctccgc caattctcgc agcgcacacg ctgccatatc
     5641 ctcgcccgcc acatccagct tgcccgcagg aagttccaat gtcgcctgat ttgccgcata
     5701 ccgccactgc cgtaccaaaa ccactttccc ctcgtccgta accgccaaca cgcatgccgc
     5761 accgggatga cggatgacga tacgctgccc ttcgttaccg ttgggcaacc tgaccttatc
     5821 cttgctgatg ctgacgaaac cgccctcgta aatggtttcg ccgcctaatt ttacttccct
     5881 caaatccact tcatatctcc tttccgtttc ctattctgcc gttcaatctt cacgcttgcc
     5941 gcgctcgatt tccacgccaa cctcgcgcac gccttccaaa atacccggct tgacgatttt
     6001 cacgcgcacc cacaccgcgc cgaaatatcc caaaaccaaa tcggcaatat attccgccaa
     6061 cgcttccaaa agcaggaaat cctgttcttt cagatgtcgg cgcagcgttt cgcatacctc
     6121 ggcataatgc accgtattgg caatatcgtc gtccgaaccc gctttctcgg gaacgccgat
     6181 gtccaaatcg acaatcaggg tctgcaaccg ttcgcgttcc cagccgtaca cgccgataag
     6241 cgtatctgcc ttcatgccgt gcaaaaagat tttatccata atccgccccg ttttttgcta
     6301 aaataaccgc accattctaa gtaaagtacc gcaaatgttc aacataccgg ctgttgctgt
     6361 ttcctatctg atcggctcac tttctttcgc cgtcatcgtg tccaagtatt acggcatgga
     6421 cgacccgcgc acctacggat cgggcaatcc gggggcaacc aatgttttac gcagcggcaa
     6481 aaaaaaggcg gccgtgctga cgctcttggg cgatgccgcc aaaggtttgg ttgccgtttt
     6541 gcttgcacgc gtgcttcaag aaccgctcgg tttatccgac agcgcaatcg ctgccgtcgc
     6601 actcgccgcg ctggtcgggc atatgtggcc ggtgtttttc ggatttaagg gcggcaaagg
     6661 cgtggcaacg gcattgggcg tgcttttggc actctctccc gcaactgcct tggtctgcgc
     6721 gttgatttgg cttgttatgg cattcggctt caaggtgtcc tcccttgccg cattaaccgc
     6781 cacaatcgcc gccccccttg ctgcgctgtt ttttatgccc cacctttctt ggatttgggc
     6841 gacgctcctt atcgccctgc tggtgttgtt ccgccacaaa agcaatatca gcaacctgct
     6901 taaaggcaaa gaaggcaaaa tcggcgaaaa acgctgattc ggcatttgtt ttccattacg
     6961 tcatgccgtc tgaaaccata tttccgcgtt cagacggcat ttccgcacaa acggctttca
     7021 taccatgctc agttacagac acgcattcca cgccggcaac cacgccgata tgctcaaaca
     7081 cttcaccttg tttttagtgc tgcaatattt caaccgcaaa gacaagccct actggtacat
     7141 cgacacgcac ggcggcgcgg gtgtgtacaa tctcgaaggc agcgaggcac aaaaagtcgg
     7201 cgaataccgg caaggcatcg ccctacttcg acaagcaaaa aacctgccaa ccgagttaaa
     7261 cggctttatc gcgcacatac aaaaaatcct gccctcgccc aaactttact gcggttctcc
     7321 gtggctggcg caatcgctga cccgtgtcgg cgacaaattg cgcctgtttg agctgcaccc
     7381 caccgacttt gtccatctgc aaaacaatat gggcgaagca ggattgggca aacgcggaca
     7441 agtgttgcgc gaagacggtt acaaagagct gatttccctg ctgccgcccc cgccgcgccg
     7501 cgccaccgtc ctcatcgacc cgccctatga ggaaaaacag gactaccggc gtgtaacgga
     7561 aacgctgaaa gccgctttaa aacggtttga gtccggctgt tatctcattt ggtatccctg
     7621 cctcagccgc gaagaaagcc gcaagctgcc cgaagaattg aaaaaactcc tgcccgacaa
     7681 ctaccttcac gccgaacttc acgtccacgc gccgaaagcc gacggtttcg gtatgcacgg
     7741 cagcggaatg ttcgtcatca atccgcccta tctgttggcg gaacaattgg cggcaaacct
     7801 ccccgccctg acgcggctgc tggcgcaaga cgaaggcgcg cgctaccttt tggacagcaa
     7861 aatccgctga ccccgatgcc gtctgaaacc cttcagacaa catctcccgt caaataaaca
     7921 aaaccggacg atataccggc agtcggcact tttaagaata aatatcccct catccgcctg
     7981 ccgcgttccg acttgcccca tgccgttttt ttacactaca atcccctaga ctatcctgca
     8041 aaacgcacca aaggaatacg ccacaataac ctgccgcctt tcagacggca caaacctttc
     8101 cgaaacgagc aaactatgga cacactgacc cgattcctcc ccgaacacct gcaacaaaac
     8161 cagctgcccg aagcactcgg cggcgtttta ctgtccgttg tttccgcctg caccgaaatc
     8221 aacgccaaag tccgcctcgg cgcactggcc ggcgtattgg gtatggcggg cacgggcaat
     8281 attcagggcg aagaccaaaa aaaactggat gttatcgcca acaacatcat gattgacaca
     8341 ctcaaagcca accctgccgt tgccggtttg gcaagcgagg aagaagactc tttcgtaagt
     8401 gccggtgaaa acggacgcta tctcgtccta ttcgaccctt tagacggatc gtccaatatt
     8461 gatgtcaaca tttccgtcgg tacaattttc tccatccttg aaaaacccga aggcgcattg
     8521 gcaaccgaat cattccttca aacgggcaga cagcagcttg ccgctggtta cgttctttac
     8581 gggcctcaaa cccagctcgt attcacattc ggacacggcg tatatatgtt cacgctcaat
     8641 gccgaaaacg aatttgtgct gacaaaagaa aaaccgaaag tacccgaaag taccaaagaa
     8701 tttgccatca atatgtccaa ccgccgccac tggctgcccc ccgtccaaca atacatcgac
     8761 gagctcttgg cgggcgaaac cggcacgcgc ggcaaaaact acaatatgcg ctgggtagcc
     8821 agcatggttg ccgaaatcca ccgcatactg atgcgcgggg gcgtgttcat gtatccgcaa
     8881 gacaaacgcg acccgtccaa acccggcaaa ctgcgcctga tgtatgaagc caatcctatg
     8941 gccctgatac tcgaacaagc cggcgcatcg gcaagcaacg cccgtcagga catactcggc
     9001 atccgtcccg aaagcctgca ccagcgcgtc gccgtcatta tgggcagcag cgaagaagtg
     9061 gattacctca accgcctgca ttcaaaataa aaggcagcct gaccgataaa gaaaggcggc
     9121 agcgcgcatc cgacgctgcc gccgatattt cgtttatagc cttaaaaaga tagaaaacca
     9181 cctttccgaa cggcaaaaat gccgtctgaa aagttttcag acggcatttt aaaaattaaa
     9241 gcccgaattt atttgcccgc tgctttttgc aggatggcgt acaactcgtc tttcagtttc
     9301 aatttggctt ttttcagctc atcgatggtt tccgcaccgc tggtaaccgg attgttgacc
     9361 agaccggtaa ttttatcgtc cagctcgttg tgttcgtcga acagacgggc gaagcgggaa
     9421 ttttcctgtt tcaatttgga aatcaaatca cggtattctg ggaacatatc gctttcctct
     9481 cttggtttac ggttaaaaag tcaaactgaa tctttacatt aattataaca aagataatat
     9541 ttttatccag tgttttatcg gatacggcaa aattttcttt taacaccgcc gtccggttga
     9601 taaaaacgtt gacaatatca cgctttacgc ataaattttc agcctttcct a
//
LOCUS       DAWFAD010000068         9650 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWFAD010000068 DAWFAD010000000
VERSION     DAWFAD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.068"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..545)
                     /locus_tag="WGO49_001227"
     CDS             complement(<1..545)
                     /locus_tag="WGO49_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0306337.1"
                     /translation="MFCTVSHLRRLKGHDVLLTAFARALAQCPQLRLNIGGSGQEEQR
                     LKQQAADLGITHAVTFLGALQPEAVLDLMRNSDAFVLASRTETFGVVYIEALSQGLPV
                     IATRCGGAESIVSDGNGYLVPVDDDDALADALIKMYEHHSDFEPARLRENCLNEFGEN
                     AVIGRLIGIFRQAIAEYGKKIR"
     gene            complement(577..960)
                     /locus_tag="WGO49_001228"
     CDS             complement(577..960)
                     /locus_tag="WGO49_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0306338.1"
                     /translation="MYFFPRFPFIDIDCIRWVCAGLKAFKHYIRENGLPDLIHAHRMN
                     YAGILAQKISQKYGIPYVVTEHSSTITRGLIRPHQWQPMEKAAAHASALLAVSRHFAH
                     VLQHKYGCEWQYLPQHTGRNIQPTV"
     gene            1329..2033
                     /locus_tag="WGO49_001229"
     CDS             1329..2033
                     /locus_tag="WGO49_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ0306339.1"
                     /translation="MLRSILAASLLAVSFPTAAEALNYNIVEFSESAGVEAVQDTMSA
                     RFQVTAEGRDKNAVNAEFVKKFNNFTRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAVLRFK
                     ARAEKLAGVLGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     PGVEEISISVNGTVQF"
     gene            complement(2097..2663)
                     /locus_tag="WGO49_001230"
     CDS             complement(2097..2663)
                     /locus_tag="WGO49_001230"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ0306340.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2729..3145)
                     /locus_tag="WGO49_001231"
     CDS             complement(2729..3145)
                     /locus_tag="WGO49_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306341.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(3197..4096)
                     /locus_tag="WGO49_001232"
     CDS             complement(3197..4096)
                     /locus_tag="WGO49_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ0306342.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4127..5584)
                     /gene="der"
                     /locus_tag="WGO49_001233"
     CDS             complement(4127..5584)
                     /gene="der"
                     /locus_tag="WGO49_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ0306343.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5738..6367)
                     /locus_tag="WGO49_001234"
     CDS             complement(5738..6367)
                     /locus_tag="WGO49_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ0306344.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RATSQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISTAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6368..7663)
                     /gene="hisS"
                     /locus_tag="WGO49_001235"
     CDS             complement(6368..7663)
                     /gene="hisS"
                     /locus_tag="WGO49_001235"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ0306345.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            complement(7789..8331)
                     /locus_tag="WGO49_001236"
     CDS             complement(7789..8331)
                     /locus_tag="WGO49_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0306346.1"
                     /translation="MQSWKARRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVRQIRQARAE"
     gene            complement(9448..9648)
                     /locus_tag="WGO49_001237"
     CDS             complement(9448..9648)
                     /locus_tag="WGO49_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306347.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         2264 a         2320 c         2457 g         2609 t
ORIGIN      
        1 cgtattttct taccgtattc tgcgattgcc tgtcggaaaa tgccgatcaa cctgcctata
       61 acggcatttt cgccaaattc gttcagacag ttttccctaa gtcgggcagg ttcaaaatca
      121 gagtggtgtt catacatttt gatgagtgcg tcggcaaggg catcgtcgtc gtcaacagga
      181 accaaatatc cgttgccgtc tgaaacaata gattccgcac cgccgcagcg tgttgcaatg
      241 acgggcaatc cttgggacag tgcttcgata tagactacgc cgaaggtttc ggtgcggctg
      301 gcaaggacga atgcgtcgct gttcctcatc aaatccaaga ctgcttcggg ctgcaatgcg
      361 cccaaaaatg taacggcatg ggtaatgccc aagtctgccg cctgctgttt cagccgctgt
      421 tcttcctgtc cgctgccgcc gatgttcagg cgcagttgcg ggcattgtgc caacgcccgg
      481 gcaaaggcag tgagtaggac atcgtgtcct ttgagacggc gaaggtgcga gacggtgcag
      541 aacacgaaat gcggtttgtt gtttttttct ctttgttcaa acggtcggtt gaatattccg
      601 cccagtatgt tgggggaggt actgccattc gcagccgtat ttgtgttgca ggacgtgtgc
      661 gaaatggcgg ctgacagcga gcagtgcgga ggcgtgcgcc gctgcttttt ccatcggctg
      721 ccattggtgg ggacggatca gaccgcgcgt gatggtgctg ctgtgttccg tgacgacata
      781 ggggatgccg tatttttggg aaattttttg ggcgagtatg ccggcatagt tcatgcggtg
      841 ggcgtgaatc aggtcgggca gcccgttttc gcggatgtag tgtttgaaag ctttcaaacc
      901 cgcacacacc cagcggatgc agtcgatgtc gatgaacgga aagcggggga agaaatatat
      961 gccgtgccat gcatagatgt ccaaaccgct ttgccgatat tttgcaaaac cgtaaggccg
     1021 gtcaggatgc ttgctgtttc tttccgcaag tagcggaaca tcggtgcaag cacggcggtt
     1081 ttgatgcctt tcctctgcaa tgccagtgcc tgattttgaa aaaaatcccg tccacatcct
     1141 gttcggattg cggataccat gaggggatga cgaggacgtg caagggttcg ggcatagtgg
     1201 gattccgtat cggaaaggcg gttattataa gacagacgca gaccgaatat ttaaattgtt
     1261 gccttacgct aatgcaattt agcgcgccga tatgctagat tggcggtttt ataagtaagg
     1321 atacggatat gttgcgttct attttggcgg cttccctgct ggcggtatct tttccgacgg
     1381 cggctgaagc attgaattac aatattgtcg aattttctga atcggcgggt gtcgaggcgg
     1441 ttcaggatac aatgtccgca cgtttccaag tgacggcgga aggacgggac aaaaatgccg
     1501 tcaatgccga gtttgttaaa aaattcaaca atttcaccag aaaatcaaaa aatggtagct
     1561 ttaaaaccga attggtatcg cgcagtgcga tgccgcgcta tcaatatacc aacggcagac
     1621 gcattcaaac aggttgggag gagcgtgcgg aatttaaggt cgagggtagg gattttgatg
     1681 cgttgaaccg ttttattgcc gatgttcagg cagatgccgc gttggaatat acggatttcc
     1741 atgtgtcgcg cgaacgccgc aacgaggtca tcgatcaggt cagcaaggat gccgttttgc
     1801 gtttcaaggc gcgtgccgaa aagttggcgg gcgttttggg tgcgtccggt tataaaatcg
     1861 tcaaattaaa tttgggacac atcggcagcc atatcgcggg agggggagct gctcaggcaa
     1921 aaatgcttcg tgccatgccg atggcggcaa gcgtcaatat ggagggtgcg gattccgccg
     1981 cgcctggtgt ggaggaaatc agcatcagcg tcaatgggac ggttcagttc tgatttgagg
     2041 tgaacggcaa atgccgtctg aaacccgacg ataagggttc agacggcatt tatatttcag
     2101 gctttgggca gggtaacgcc ggtttgcccc atatatttgc cgttgcggtc tttgtatgag
     2161 gtttcgcaga tttcgtcgct ctcgaagaag aggacttgcg ccacaccttc gcctgcgtag
     2221 attttggcgg gcaggggggt ggtgttggaa aattcgaggg taacgtagcc ttcccattcc
     2281 ggttcgaacg gggtaacgtt gacgataatg ccgcagcggg cgtaggtgga tttgcccaag
     2341 cagacggtca ggacgttgcg cgggatgcgg aaatattcga ccgtgcgcgc cagtgcgaag
     2401 gaattgggcg ggatgatgca gcagtcgtct tcaacggtaa cgaagttttt cgggtcgaag
     2461 tttttgggat cgacgatggt gctgttgatg ttggtgaaga ttttaaattc gtttgcgcag
     2521 cggatgtcgt agccgtagct ggacgtaccg taggagatga tgcgtttgcc gtcggcttct
     2581 ttgatttggt tcggctcgaa agggtcgatc atgccgaatt cttcgctcat tcggcgtatc
     2641 catttgtcgg acttgatgct cataatgttt tccttgtttc ttgcagtgtt cggacaaagc
     2701 attgggggat gccgtctgaa aacggggctt atttgttttt gggcagcttc acttctttaa
     2761 tcatgccgtt ttcgcatttc ataatgaaga gggtttcgcc gttttgcgag acggtaaatc
     2821 tgccgtgtgc caagcctttt ttgaatgtgc ccgagagtac catgttgcgg aatttggtac
     2881 tgtcggaatt gaacggttcg ataaatattt cgcggttggc ggcaacggta taaacgcctt
     2941 gtccgtcgaa tttgccattt ttaaacgaac cggtatagtt gcgcccgtct tggcagcgcc
     3001 atgtgccttt gccggcgggt ttaccgtctt tgccgacatc gccttcgtag gtgcagccgg
     3061 gttcttgata ggaagtcaga acggcggacg cggcggggag ggcgaacatc atggcgggca
     3121 gtaggaatgc gagatgttta agcataaggg ttattccatt ggattttggc tgacggtata
     3181 aaagccgtct gaaaaatcaa tcttgccagc cgcccaaata ggaaaccagc tcttccaaca
     3241 tgatgctgac ggattccgcc atcaggattt gcgaggcgaa ggcgagaccg gcggcatcgt
     3301 cgccgttgct ttcggcttct tcctgcaata cgtcgaggta ttggatgcgc ttgagtgtga
     3361 agtcttgagt gaggataaag gcgatttgtt cgcgccaaac caaaccgagc tgggttacgg
     3421 ttttaccgtt tttgacgtgt tgaaccactt cgtcggcggt taaatcttgt ttggatactt
     3481 tgacgacggg aacaatatcg cccgtgcctt tgagttcgca atcgctgtcc aattcaaaac
     3541 cgccttcgca atgcccttgc aacagccagc cggtcatcaa ggaagagggc gattgcttgg
     3601 tattcggcag cgaggcttcc aaaccgccca aagcttcgcg cagcttggtc aggatgtttt
     3661 ctgctttggc ggaagccgcg ttattgacga gcaggtagcc gtggcgggtg ttaaacaccg
     3721 cttctgtacg gctgctgcgg gtaaacgctc ggggcagcag gtcgtctgta atttgctctt
     3781 taagctcttg tttttcttta cggccgacat tgcgggcttc attgttttgg atttccgcta
     3841 ccttctcttc caaaatatcg cggatgacgc cggcaggcag gactttttct tcttttttca
     3901 gggcgacgcg caaggtaaag tcggcaggga aaacgagttc aggggagaat gaaaccggtg
     3961 cggtaaagcc ttcgctgaac cagtctaagc cttggcaatg ggtaaattca gcttcagcaa
     4021 gtttgtcggc aagtacgtct gcctcaggca gcttttcttt gttgagcgga taaaaactaa
     4081 tctgcttgaa ccacataatg tttcctattg tttgaaatgt cgggaattat ttgctgaatt
     4141 gttttttcac actgactttg gttttcttct tgaagcggtt tttctcttct ttgcggcctt
     4201 cgcgtttctc aatacggttg ctcaggctga cgcggcgcag cggttttttc ttcggtttgt
     4261 cttccgcatt ttcatacggg ttttccgaaa cattgtattg aatccgcaac ggcgtgcctt
     4321 gcaggttgaa ggctttgcgg aacgtttggg tcagatagcg cgtatagctg tcggaaatcg
     4381 cgtgcagcga attgccgtgt accacaatta cgggagggtt catgccgcct tggtgggcat
     4441 aacgcatttt cggacgcacc aagccggcac gcggcggttg ctgacgctcg atcgcgcttt
     4501 gtaatacgcg cgtgattttc ggcgtcggca tcttaatcat cgccgcgttg taggcagcct
     4561 gaatgctgtc aaacaaaccg tctataccgc gctctttcaa tgcggaaata aagtggaact
     4621 tggcaaaatc gaggaaatac agtttgcggt tgatatcgcg tttcacttgc tcgcgacgct
     4681 cttcgctgat gccgtcccat ttgttgacgg caaccaccaa ggcgcggcct gcttccaaag
     4741 caaaaccggc aatcgtcgca tcttggtcgg cgatgtcctg ctgcgcgtcc aataccaaaa
     4801 cagcgacgtt tgccgcttca accgcctgca tcgctttgat aacggagaat ttttctactg
     4861 cttcatccac tttgccgcga cggcgcacgc ctgcggtatc gatgatggta aacggtttgc
     4921 cttcgcgttc gaaatcgatg tggatgctgt cgcgtgtcgt gcctgccata tcgaaagcaa
     4981 tcacgcgctc ttcgccgagg atggcgttaa ccagtgtgga tttgccgacg ttcggacggc
     5041 cgatgacggc aaagacggga tgttttgcat cggcttcttc ggcttccggc tctgggaagg
     5101 tttccaaaat atcttcaatc agataataca cgccatcgcc gtgcgcacct gaaataacat
     5161 aagggtcgcc caaagcaagt tcgtagaact cggcggcaag cacggccctg ttgccgcctt
     5221 cgcctttgtt taccgccaga taaacagggc gcgggctttg gcgcaggcgg tcggcaatga
     5281 ttttgtcttg cggggtcagg ccggtacggc cgtccaccaa aaacacaacc gcatcagctt
     5341 catcgacagc ctgcaaggtt tgttttgcca tttcgtgcaa aatgccgctg tccacaaccg
     5401 gctcgaaacc tccggtatcg atgaccaaat aaggtttgct gccgactttg ccgtgtccgt
     5461 aatggcggtc gcgggtcagg ccgggcaggt cgtgtacgag tgcgtctttg gtgcgcgtca
     5521 aacggttgaa taaagtagat ttgccgacgt tagggcggcc gacaagcgcg atggtgggtt
     5581 tcattattgg gtctttctgt gtcaagtgcc gttcgggaga actgaacacg agcaggtgtc
     5641 cgttggacac ggtaaatagg ttcttcacgg caagaggcca taaagaggga gggtaaaatg
     5701 ccgtctgata aataatggac agcatttcag acggcattta tttcagcgaa tcaagtttca
     5761 tttgaaccaa ttcgcgaccg acagaatctt gaggcatttt ttctaaagcc tgtccgtagt
     5821 tttttaaggc ttcctggctt tttccctgtg cggcatagac atcgcctttg gtttccatca
     5881 gcaggggggc gaagtccgct tcaaccggcg tatcgagcgc ggcaagcgcg gcatcgtatt
     5941 ttttttgttg caacaacaca acgcccagac gctgcgccgc caacgcttga atcaggctgt
     6001 ctttttggtt ggacaacacc catttcaaat ggccttcggc aacatcgtaa cgctgtgcgt
     6061 caaattcggt tgccgccgcc atcagcgtgg cttgggcggt ggaaatggaa tgcgggtagc
     6121 tttgctggag tttggtcaat tcggcattga tttcgctttg cggggctttg ttttgcgcct
     6181 tttccacgat gtttgccagc accgccgccg cttcctgatt ttgggaagtc gcacggtttt
     6241 ggtaaaccgt gtatcccaag tagccgagcg ccgccaaaat cagcagggca aacagccatt
     6301 tgcccgtggt tttccaaaaa tatttaaagt tgtccaactc ttgttgttct tccaaatggg
     6361 ctgccattta tgcgttcttc cattgttgta aagtgtttgt caaatcagcg gcggcgacag
     6421 tttgctgacc gtgtgcgccg ttcatgtctt tgagcgtaac cgtaccgttc gccagttcgt
     6481 cttgcgccac aatcagggca aagcgcgcgc cgctgttgtc ggcttttttc atttgtgctt
     6541 tcaggctttg atagccggaa tgctgcatta cattgaaacc ttgcgcgcgc aaggcttgcg
     6601 cgtatttcat cacctgcaag tccgcccttt cgccttggtg cattgcatag acatcaggcg
     6661 cagcgttcac ttccagcgag ccgtattcgc tcaccaaaag cagcagccgt tcgatgccca
     6721 tcgcaaagcc gatggacggc gcaggtttgc cgccaagttc ctcaatcaaa ccatcgtaac
     6781 ggccgccgcc gcacacagtt gcctgcgcgc cgagtttgtc ggtcgtccac tcaaaaaccg
     6841 tctgattgta ataatccaaa ccgcgaacca agcgcggatt ttcaatatat tggataccca
     6901 aaccgtccaa catcgccttg aagcgtgcat agtggttttg cgaagcctcg cccaaataat
     6961 ccaccaaacg cggcgcggcg ttgcagattt cctgcaaatc agggtttttc gtatctaaaa
     7021 cacgcaaagg attggttttc agacggcgtt tgctgtcttc gtccaattta tcttcatagc
     7081 gtgtcaaata ttcaaccaac gccgcacggt gtgccgctcg ctcctcacgg ttgcctaagc
     7141 tgttgatttc caaagtcagg tattcgcgga tacccaattt ttcccacaag tcggcagaca
     7201 tcgcgatgat ttccgcatcg atatccggcc cttcaaaacc caaagcctcg ataccgacct
     7261 gatggaattg acgataacgg cctttttgcg gacgctcgcg gcggaacatc ggccccatat
     7321 accacagctt ttgcgggctg ttgtacagaa ggttgtgttc gaccaccgca cgcagacaag
     7381 aagccgtacc ttccggacgc aggctcaaac tcaaagaatc gtttgaatcg gaaaaggtgt
     7441 acatttcctt gccgaccaca tcggtttctt cgccgatgga gcggacaaac aaaccggttt
     7501 gctcgacaat cggcgtacgg atttgctgat agccgtaagc gcgcgtccag cggtttaccg
     7561 tatcctcaaa cgcctgccaa aacgcagccg tcagtttgaa atctttttgc tcgacaggca
     7621 gaaggtcgtt catgcctttg acggattgga ttttttgtgc catttcaagt aagaatgctt
     7681 aaatcaaatt gcgggcgatt atagcggatt tttaagggtt tgtgaggttg ggggtggttt
     7741 gcggacgggt aaaatgccgt ctgaaacagt tttcagacgg catttgactt actctgcacg
     7801 tgcttgcctg atttgtcgga ctgcaaactc tgtctgccgc ttgggatgat gtgtgaaaaa
     7861 caatttattt gaaattgtct cggctttttt cggcacgacc gccaaaatct tacctgccaa
     7921 atttccctca cgggtttgcc aagcatccaa aaactgcgcc ctgctcatcg aaacatgacc
     7981 cagcgacggg tcggcaagca aaaccgtatt gccgtctata ccgcgcaata ccgagaaatg
     8041 atcatcctta cggtatttca gatacacgat gacggggatt ttcaactgcg cgagctgctc
     8101 gaaagacagg gcatagcctt tcgcttcaaa acccaaatct ggcataatgc gccgcatatc
     8161 ctcaaacgac gcgcgcatct gctccttatc cagctttttc aacacttctt cttccgtcag
     8221 cgtttgcccg taaaaattgt tcaaaagcgt cgccaccgaa gccgccccgc aggaaaaatc
     8281 caaatcctgc tttacaatat tgaaatcccg ccgcgctttc caactctgca ctttgatttt
     8341 tccgtaaaca acaggattat cgttaaacat cggtgcagca ttcagacgat aagataaaga
     8401 aacgacaaca cacgccaaca gaaaaacata tttgaacttc atcatattgt ccacataaag
     8461 ggcagcctga aaatctttca ggctgccctt gtcaaattat tcctagcttt cggctttttt
     8521 ggcaaaccaa acaatccgat tacccgcata atactttcca tttattgaaa tccgacaagc
     8581 cgcgcccaaa aatgccatgc actgtcgatt tccgcagcaa tctttgtacc gttttcttca
     8641 aattccaaat attcacccaa taataaactt gaaacagaac gcgtgggcag caagtgccca
     8701 ccctacgctt attcaaataa tttgattaaa taaagaataa agaaaatgca tcaggaacaa
     8761 aattataatc tgccacctga ctcacaccgc ttttaaagta aggggcatca aaatcaaaac
     8821 cgcaaaaaaa ataatttttg cattgatttt taatagattt aaaattcgaa tatagtgttt
     8881 ctccagattt aaaaagttta tctttatcta tatatttgat gctttcccta tccaaaataa
     8941 atattcaaac attaaaaaat cattacatga ccaagccaaa tcataaattt gattcaaatg
     9001 ggtatcataa agataaaaat aataaggttt gggaacaggt aaaatattta atggaaaagg
     9061 aggactaatt ttcttaatat ctgatgactg atatccatat ctttctatat tcttaaaaat
     9121 ttcatctttc ttaagataac aagacattct gaagataaca agacattctg acaatcacaa
     9181 cctgttcatc ggatatatta tctatttgca tggcgcgtat aacacgccat gcctgattaa
     9241 aattagtctc ccttacctta gaatctatta tcttttccaa agatgaaaat gccccttatc
     9301 ttgaactctc cagttagaac ctggaagttt cgtacctctt aaatgtgtat taatatttct
     9361 tatagtttca ttaaaatgcc acgcgctgcc gatttcaacg gtaattttcg taccgctttc
     9421 ttcaaattcc aggtattccc ccattagcta acgcaaagaa gcagacgcca tttcggcttc
     9481 gttatgataa acccgccttc cgttgatata gacttccgcc cctgtccgct ccaaattcca
     9541 aaaattccgt actgaaattt ccatatcccg atattgtgca gaccatgttt tttcgaaggt
     9601 tttcataaaa tttcctatac ctgtccaatc ggcacatatc aattgcatta
//
LOCUS       DAWFAD010000069         9557 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWFAD010000069 DAWFAD010000000
VERSION     DAWFAD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.069"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            41..196
                     /locus_tag="WGO49_001238"
     CDS             41..196
                     /locus_tag="WGO49_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306348.1"
                     /translation="MHKLCLATSRSDDFIRKTAASGTISNRPTEDGILSTRPAAYAAE
                     EPLYQAS"
     gene            205..1254
                     /locus_tag="WGO49_001239"
     CDS             205..1254
                     /locus_tag="WGO49_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0306349.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKGETRKTATQAY
                     GQKASDGIKPQNAPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWITNGWITVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGEYRRKKA"
     gene            complement(1323..2864)
                     /locus_tag="WGO49_001240"
     CDS             complement(1323..2864)
                     /locus_tag="WGO49_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ0306350.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGTSAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSIGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYGVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(3157..4512)
                     /locus_tag="WGO49_001241"
     CDS             complement(3157..4512)
                     /locus_tag="WGO49_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ0306351.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSILENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVAGKISIYETRGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPARPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGTGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKDTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            4709..5530
                     /gene="nadE"
                     /locus_tag="WGO49_001242"
     CDS             4709..5530
                     /gene="nadE"
                     /locus_tag="WGO49_001242"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ0306352.1"
                     /translation="MMRIFSTGGKMDTQAVITHIARWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRPTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGIHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(5598..6071)
                     /locus_tag="WGO49_001243"
     CDS             complement(5598..6071)
                     /locus_tag="WGO49_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0306353.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLAYMQNELLMHMMKEERMLFPMINQGVGRGAAMPISVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            6369..6701
                     /gene="trxA"
                     /locus_tag="WGO49_001244"
     CDS             6369..6701
                     /gene="trxA"
                     /locus_tag="WGO49_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ0306354.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
     gene            complement(6754..7701)
                     /locus_tag="WGO49_001245"
     CDS             complement(6754..7701)
                     /locus_tag="WGO49_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0306355.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMRPEEYEAWLAEVLEAVAFVTGFEPEQIRLKRRERQKGLQQ
                     YEKTGKAGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            8029..9417
                     /locus_tag="WGO49_001246"
     CDS             8029..9417
                     /locus_tag="WGO49_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0306356.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQRLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPTDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGRGERRYKDR"
BASE COUNT         2332 a         2659 c         2534 g         2032 t
ORIGIN      
        1 ttgaaagatt ctacccccgc acgcaaaccg atttcaaccg ttgcacaaac tttgcctcgc
       61 cacaagccgc agcgacgatt tcatccgcaa aaccgccgca tcaggtacaa tatcgaaccg
      121 tccgactgag gacggcattt tatcaacccg tcctgccgca tacgccgcag aagaaccgct
      181 ttatcaagcg agttaggaaa aatgatgtcc aaacagccca ccagcaaacg ccaatggcgc
      241 gacggcgcag ccccgtctgc caagaaaacc gccaaacctt tcaaaagcaa agcccgtccc
      301 aaaggcgaaa cgcgcaaaac cgccacccaa gcttacggac aaaaagcttc agacggcatc
      361 aagccgcaaa acgcccccaa acagcgcgcc gccaaagcca aaaaactcgt cgtccgcaat
      421 cccaaccaaa aaattatgga acacgcgcgc gatttgaaag aacgccgcag cgacctgtcg
      481 cgcatggaac ccgaacgcct gcaaaaagtg cttgccgcgt ccggcgtcgg ctcgcgccgc
      541 gaaatggaag aatggattac caacggctgg ataacggtca acggcaaaac cgcgcaactg
      601 ggcgacaaag ttacccccga cgaccacgtt accgtcaaag gcagcatcat caagctcaaa
      661 tgggcggacc gcctgccgcg catcatcctg tattacaaac aagaaggcga aatcgtttcc
      721 cgtgacgacc cgcaaggccg cgtcagcata ttcgaccgcc tgccgcaggc cgccagcagc
      781 cgctgggtcg ccatcggacg cttggacatc aacaccagcg gacttctgat tcttaccacc
      841 tccggcgaac tcgtccaacg tttcgcccac cccagcttcg aagtcgaacg cgaatacgcc
      901 gtgcgcgtct tgggcgggct gaccggcgaa caaatgcgcg tcctcaccga agaaggcgtg
      961 atgctcgaag acggcttggc aaaagtcgaa cgcatccgcg aacaaggcgg cgaaggcgcg
     1021 aacaaatggt acaacgtcgt gattaaagaa ggccgcaacc gcgaagtgcg ccgcattttt
     1081 gaaagccaag gactcaccgt cagccgcctc gtgcgcgtcg gcttcggtcc catcggactg
     1141 cccaaccgcc tcaaacgcgg gcagttctac gaactcaacc ccgccgaagt cgccaacatc
     1201 ctcaaatggg cggaaatgct gctgccgggc gaataccgcc gcaaaaaagc ctaaacccgc
     1261 caaaacacaa aaatgccgtc tgaaacatct gctgtttcag acggcatttt attcgggcgt
     1321 tttcaggaga aaaggtcgag tgctttgaca aagaccatca ccacgccgta ggcgagtggt
     1381 atgacggcca atgcccaacg ccaccagacc gatatgccgc cgccggaaac tttttcgccc
     1441 acaaggtaag cgtcggatat ggcggtttcg tcgtcggggt tgccgctgtg tgcggcggtt
     1501 ttgatgtctt tttcgtggtg tttttcgtgt acggatttga cggcgaggtt gcacaacaaa
     1561 ccgataatca gcaggcacgc catgatgtac atggttacgc cgtatgcctg tgccgccggt
     1621 atgccgctgt cgatttggct ttggcgtatg taattgacca gtaccgggcc gatgacggcg
     1681 gcggttgacc aggccagcag gatgcgcccg tgaatcgcgc cgacctgata ggtgccgaac
     1741 aggtctttca ggtaggcggg aatggcggca aatccgccgc cgtacatgga aataatcacg
     1801 caaaagccga tgatgaacag ggctttgctg ccgccctcgc cgatggaggg aacggcgaaa
     1861 tacagcagcg agccgagtac gaagaagatg gtgtaggtgt ttttgcgtcc gattttgtcg
     1921 gaaacgctcg accacaaaaa gcgtccgccc atgttaaaca ggctcaggag gctgacgaag
     1981 cctgccgccg cacctgcgcc gactgctgcc tgcctgccta tggaggtttc ggaaaagagt
     2041 tcctgaatca tcacggatgc ctgacccaat acgccgatgc cggcggttac gttcaggcac
     2101 aatacccaga acaacagcca aaactgcggc gttttcatgg cttgggacac gttgacatga
     2161 ttactgctga ccagcttgtt ttgcgttttc ggcgcggtat agccttcagg tttccagccg
     2221 tcggcaggta cgcggatggt aaacgcgccg aacatcatca gtgcgaggta aagcaggccc
     2281 aatacggcaa aggtttcggc aaccccgacc gaagcggcgt ttgaaaaggc gttcatcagt
     2341 gatacggaaa gcggagaggc cagcattgcg ccgccgccga aacccataat cgccaaaccg
     2401 gtcgccatac ccggcttgtc gggaaaccat ttcatcagtg tggaaaccgg cccgatgtag
     2461 cctaagccta agcctacgcc gccgatgacg ccgttgccca aatagagcag gaagaggttg
     2521 tgcgtacgca cgccgaatgc ggatacgaag aagcccaggc tgaaacagca ggcggcggca
     2581 aatatggctt tgcgcggacc tacccgttcc atccatgtgc cgaacagggc agccgacgtg
     2641 cccagcatcg cgagtgcgat actaaaaatc caacctacgg tcgtcagctt ccaatctccg
     2701 gccgccgatt cggttatgcc gataagtttg gtcagcggcg cgttgaatac ggaataggcg
     2761 taaatctgcc cgatggcaag gtgcaccgcc aatgctgcgg gcggtacgag ccaacggttg
     2821 aaacccggct tggcaatgct tgcctcacgg tctaaaaact tcataacatc ctctttctgt
     2881 cagttgaaaa ataaaatttc atttgcccaa tggaaactta ttgaaaatta taaaaaaata
     2941 tcggatcggt tttttatccg ccccaagatg ccccgtctga aacatttcgg gtgtacggaa
     3001 aggtttctgt tttttccgac aaattcctgc ggcttttcgc ttccggattc ccgctttttc
     3061 aggaatgacg aattaaagat tatcttaagg tcaagggact ggattcccgc tttcgcggga
     3121 atgacggcgg agggggagcg gtttttccga ttgggtttaa atgcaatcga acaaatcctg
     3181 ctgcccttgt tctttgctta cgcgcacgtc ggtttcgccg tcggcgaagg taatgtgcag
     3241 cttctgcccc tgcttcaaaa catcggcgtt gcggatgact tgtccgcgtg tatctttgac
     3301 gacggaaaag ccgcgttcca aaatgtgctg cggcgaaacg gcttcgagca atgcggcttg
     3361 ggcggtcagg ctttggcggc ggtgggtaag cagttggctg aaggcggcgg gcaatgccgt
     3421 ctgaaggcgg acgatgttgt ttttgcaaac ggaaacatca ggacggtaat gttgcagggc
     3481 ttgggtttgg cgttcaaaac gggcggtgtg ggcacggagg ttttgcttca tagagtaaga
     3541 cagcgtttgc gccagcttgc cgattgaagc gcgctgctca tcaagttttt ggcgcggatg
     3601 acggatttgc cgcgcaagcc agtcgagttt ttggctggca tcgaaatagc gttgttccaa
     3661 aacggttttc agacggcctt gcgcctgagc gagacgatgt agcgattctt ggcggttggg
     3721 gctgaccagc tccgccgcac cggtcggcgt gggcgcgcgc acgtcggcga caaaatcggc
     3781 aagcgtgaaa tcggtttcgt gtcccacgcc actgacgacg ggaaccgtgc aggcttcgat
     3841 tgcgcgcacg accggttctt cgttaaacgc ccacaagtct tcaatgctgc cgccgccgcg
     3901 acagacaatc aacacgtcgc attcggcgcg ttgcgaggcg gttttaatcg cttgtgcgat
     3961 ttgtaactcg ctgcctgtgc cttgaacagg tgtcggataa acgataacgg ggatttcggg
     4021 tgcgcggcgt ttcaaggtag taacgacatc gcgcaaagcc gccgccgcca gactggttac
     4081 gatgccgata cattgcggtc gggcgggcaa aggtttcttg cgttccgccg caaacgcgcc
     4141 ttccgcctgc aactgcgcct tcaaccgctc gtaggcttcg taaagctgcc ccaagccctt
     4201 gagccgcact tcgttcacgg taatctgaaa ttcgccccgc gtttcataaa tactgatttt
     4261 gcccgccact tcgatatggt cgccttcttt caaaggtttc gccaaacgcg ccgccgcacc
     4321 cttgaacatc gcgcaacgca cctgcgcgcg gctgtctttg agcgagaaat aataatgccc
     4381 gctggcggca cgggtcaggt tggacacttc gcccgcaatc cacagaccgg caaggtggtt
     4441 ttccaaaata cttttggcaa atgcgttcaa ctcggaaacg gacaacacgt cagaatggaa
     4501 aaaatcagac atcgaatcaa tcaaatagta aaaaatatga atatgttttg aagcccaagg
     4561 cggcatagag ccgcctaaat tgtcaacaat attataacac gcgccatctt gccgcccgcc
     4621 ttttcccata tgactttttt aagcggggaa tgggaaaaat attcatcaac ctgcctgcaa
     4681 tctattcaaa ttgaaccgcc gacaagctat gatgcggata ttttcgacag gaggaaaaat
     4741 ggatacgcag gcagttatca cacatatcgc ccgatggttg gacgaatacg ccgcacgggc
     4801 aaatgcaaaa gggtttgtcg tgggcgtttc cggcggcatc gattccgccg tcgtctccgc
     4861 actcgccgcc cgcaccggcc gccccacgct gcttctggat atgccgatac gccaacaccc
     4921 cgaccagctt gagcgggcaa ggctgcacat ccgcaatctg caacggcaat atgccaatgt
     4981 aagcgcgcaa acggtcgacc tgaccgacac cttccagacc tttgaacaaa ccgtcggcat
     5041 tcatcagacg gcatttgaca atcagccgct ttccctcgcc aacgccagaa gccgcctgcg
     5101 tatgctgacc ctgtactact acgggcagat acacggactg ctggttacgg ggacaggtaa
     5161 taagattgaa gatttcggcg tgggcttttt tactaaatac ggcgacggcg gcgtagacat
     5221 cagcccgatt gccgacctga ccaaaacgca ggtttaccgg cttgccgaag cattgggcgt
     5281 ggacgaggcg attcaaaaag ccccgccgac cgacggcctg tgggatacgg aacgcaccga
     5341 cgaagaacag atgggcgcaa gctatcccga actggagtgg gcaatgggcg tgtacggcac
     5401 gcgcaaaccc gaagattttg aagggcggca gcgcgaagtt ctggaaatct atacgcggct
     5461 tcaccgcgcc atgcagcaca aaatcaaccc gattcccgtg tgccgcatcc cgcccgaatt
     5521 gttgggctga acgtgaaaat gccgtctgaa gcggaaaacc gtgtttcaga cggcatggaa
     5581 atatccggac gcgctcctta agaatcgagt acgcgggcaa acagaatatc gttttccaaa
     5641 tgaatgtggt cgttcaaatc ctccaccatt tctttcgcca gcgcgtaaag ccgcgtccag
     5701 cttccgcaag ccccttctgg cggttggaaa ttgtcggtca gctctttgag ccgtgcgatg
     5761 gcgcggtcgt gttcttcgtg ttcgtgcatc atcacgctga tgggcattgc cgcaccgcgt
     5821 ccgacaccct gattaatcat cggaaacagc atcctttcct ctttcatcat atgcatcagc
     5881 agttcgttct gcatataggc aagcagcccg gcaatttccg ccggaaaggt atcggcatgg
     5941 acttgggcca ctttctgagc cagcggcacc aattcttcaa attgtgcgcg gtggacattg
     6001 tggtagcgtt gcaggatatg atcgacggtt gcaccaaagg gggcggtctc ccaaacggaa
     6061 aaatcagtca tcgcagtgtt ccttttacag ggtttcgggt ttggttttga acattcatac
     6121 tttaagaatc aattcaaacg gagcatacac cgcccgcgcg cttctgtaca gcctcaaacg
     6181 tattccttac attttgataa taaaagtaat tttcagaaat aaaatactgt ccgaaccgtt
     6241 ttttagaatt tgcaaaggcg attggggcgg tacagaaaaa ctattatccc gcccgcccac
     6301 ttgaaatttt tatgcccaag ccctatcctg cacgctatcg tgccaatccc aaccgaaaag
     6361 gaaaaataat gagcagcgaa ttgattgtac acaccagcga tgccgctttt gaaaaagacg
     6421 ttttaaatgc agatatcccc gtcctgctgg acttttgggc tccgtggtgc ggcccctgca
     6481 aaatgattgc cccgattttg gacgacattg ccgccgaatt tgaaggccgt ctgaaagtgg
     6541 tcaaaatcaa catcgacgac aacgaagcca ccccgtcccg tttcggcgtg cgcggcattc
     6601 cgaccctgat ggtgttcaaa aacggcgaag tcgtcgccac caaagtcggc gcattggcaa
     6661 aaggtcagct gaccgccttt gtcgaagcct ctatcgcctg ataaagcgca atcgaaaaag
     6721 ccgccggaag attccggcgg ctttttcgca cccttaagat ttgtggcgga tttcccagca
     6781 cctatggatt tttttgttgc ggaaatcttc gggaacggat tgtttggaaa tgtctttgac
     6841 ggcgtattgt tccgatacca agtcgtctaa gacgaagctg cgcaggttgt tggaaaagta
     6901 caaaatgccg tctgaagcga gcagcttcac cgcgccgtca atcagctttt tgtggtcgcg
     6961 ctggatgtcg aggatgtcgg acattttctt gctgttggaa aaactgggcg ggtccatcac
     7021 aatgaggtcg aaccgcctgc cttccccata tgccgtctga agatattgga acacgtcggc
     7081 gcggacgatt ttatgccgtt ccgtatcgat gccgttcaat tcaaaattgc gtctcgccca
     7141 atcgagataa gtgttagaca aatcgacagt ttcgctggat gccgcgccgc cggtggcggc
     7201 atagacagtg aagctgccgg tgtaggaaaa caggtttaaa aaacgtttgc ccgccgccgt
     7261 ttcgccgact tttttgcgcg tgtttcgatg atccaaaaaa agccccgtat ccaaatactt
     7321 atcaaggttg acccaaaact tgcggccgtt ttcggtgatg acgaaatcgt cgcccgcctt
     7381 gccggttttt tcgtattgct gcaaaccttt ttggcgttcg cggcgtttga ggcggatttg
     7441 ttcgggttca aaaccggtaa cgaaagcgac ggcttccaag acttcggcaa gccacgcttc
     7501 gtattcttcg ggccgcatca gccagccggt atcgtattcc tgaaggtgga ttcgatcgcc
     7561 gtaaacatcg gcggcaaagg ggaattgggg gatgtcgcgg tcgtaaatgc gccaggcttc
     7621 aatgccgttg cgtttcgccc atttcataag gtgtttgatg tttttgccca agcggttggc
     7681 aaacggtgtg atgtcggtca ttggtttcag gcggaataaa gtggaaaacg gcaattttac
     7741 tgtaattaac gcccgattgc ttgaccgttt cgggcaaacc ctataccatc cgccgcttat
     7801 cttgtcatac gaagccatcg ccttccaacc taaaccgccc ttacgggcgc gtttcttttg
     7861 ttgctttgat tttgcaaagc ctttctgtgc aggttgtcgt cgatgtaaac cacaagcaag
     7921 ccgcttgcga caaccctgta acttcacatt ccccgtatcg ttacccttcc ctgcttcagg
     7981 ccgtctgaac ctttcggacg cgggcgttgt tgtcttccaa ggatagccat gtctattaaa
     8041 tttgccgatt tgaaccttga taaaaacatt ttgtccgccg tcagcagcga gggttacgaa
     8101 agcccgacgc cgattcaggc gcaggcgatt ccgtttgctt tggaaggccg cgacatcatg
     8161 gcttcggcgc aaaccggctc cggcaaaacc gccgcctttc tgttaccgac tttgcagcga
     8221 ctgaccaaac gcagcgaaaa accgggcaaa ggcccgcgtg ctttggtgtt gaccccgacc
     8281 cgcgaattgg cggcacaagt ggaaaaaaac gcgctggcgt atgccaaaaa tatgcgctgg
     8341 ttccgtaccg tcagcatcgt cggcggcgca tcgttcggct accaaacccg cgccctgagc
     8401 aaaccggtcg atctgattgt cgccacgccg ggccgcctga tggacctgat gcaaagcggc
     8461 aaagttgatt ttgaacgttt ggaagtgctg attttggacg aagccgaccg tatgttggat
     8521 atgggcttta tcgacgacat cgaaaccatc gtcgaagcca ccccgaccga ccgtcagact
     8581 ttattgttct ccgccacttg ggacggcgcg gtcggcaaac tggcacgcaa actgaccaaa
     8641 gaccctgaaa tcatcgaagt cgaacgcgtg gacgaccaag gcaaaatcga agaacaactg
     8701 ctgtactgcg acgatatgcg tcacaaaaac cgcctgctcg accacatcct gcgcgatgcc
     8761 aatatcgatc aatgcgtgat tttcacgtcc accaaagcga tgaccgaagt catcgcggat
     8821 gaattgtacg aaaaaggttt cgccgccaac tgcctgcacg gcgacatgcc gcagggctgg
     8881 cgcaaccgca cgctgatgga tttacgcaaa ggccgctgca aaattttggt tgccaccgac
     8941 gttgccgcac gcggtatcga cgtaccgacc attacccacg tcatcaacta cgacctgccg
     9001 aaacaggcgg aagactacgt ccaccgcatc gggcgcaccg gccgcgcagg ccgcacgggt
     9061 attgcgatta cgtttgccga agtgaacgaa tacgtcaaag tccacaaaat cgaaaaatac
     9121 attaaccgca aactgcccga actgaccatc gaaggcatgg aaccgacccg caaacgcaaa
     9181 tccgcaggcg gtaagccaaa aggcaaaggc ggctggggcg ataggaaatc cggcggccgg
     9241 cgcggcgatc ataaaccggg caaagaaggc ttcggcggca aaacgcgcgg cgaaggtttc
     9301 aagaaagaag gctttaagaa agacggtttc aaaaaaaccg gcgaaggctt caaaggcaaa
     9361 cgtaaagccg gcgattcttt tgcaggcaga ggcgaacgcc gttacaaaga ccgctaagcc
     9421 ccaacctgcc tcataaacca atgccgtctg aaaccgattt cgagtttcag acggcatttt
     9481 tgcaatgttt caacaccgcc cggctttgat acccaaagga ttaatctgca atatagtgga
     9541 ttaacaaaaa ccagtac
//
LOCUS       DAWFAD010000070         9418 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWFAD010000070 DAWFAD010000000
VERSION     DAWFAD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.070"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            134..343
                     /locus_tag="WGO49_001247"
     CDS             134..343
                     /locus_tag="WGO49_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ0306357.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            432..1646
                     /locus_tag="WGO49_001248"
     CDS             432..1646
                     /locus_tag="WGO49_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ0306358.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(1733..4312)
                     /gene="clpB"
                     /locus_tag="WGO49_001249"
     CDS             complement(1733..4312)
                     /gene="clpB"
                     /locus_tag="WGO49_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ0306359.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSCLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMSIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            4573..5583
                     /gene="trpS"
                     /locus_tag="WGO49_001250"
     CDS             4573..5583
                     /gene="trpS"
                     /locus_tag="WGO49_001250"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ0306360.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFRELFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETVEFTKMLADGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5589..5930
                     /locus_tag="WGO49_001251"
     CDS             5589..5930
                     /locus_tag="WGO49_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306361.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            6250..6615
                     /locus_tag="WGO49_001252"
     CDS             6250..6615
                     /locus_tag="WGO49_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306362.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAKEAVTEAAKDTL
                     NKAADATQEAADKMKDAAK"
     gene            complement(6824..8332)
                     /gene="ppx"
                     /locus_tag="WGO49_001253"
     CDS             complement(6824..8332)
                     /gene="ppx"
                     /locus_tag="WGO49_001253"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ0306363.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPDGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(8449..9180)
                     /locus_tag="WGO49_001254"
     CDS             complement(8449..9180)
                     /locus_tag="WGO49_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0306364.1"
                     /translation="MSREINAGGHHFLYGGISGGGVHYWDNKDFSEQCLHLSLGYKNR
                     SVTRSFGIVPFVGQNLLGGSRYNFVGGFNADFSQHLSERWRLTLNAGNMWKHYQEDRT
                     AARYDSHMPLAGATLMYSAPKDWLLYGGADWSHDLTKEAEQASVRKGLRIGAVKTFDG
                     GLGLRANLRYTRRTFDAPGTIVYRFPRKDHEYQANLSFWHDKISWKGFTPQLNFRYLK
                     IDSNMKSFYTRKNTQIFMSVEKDFK"
BASE COUNT         2174 a         2440 c         2484 g         2320 t
ORIGIN      
        1 tgcggcttcg tcgccttgtc ctgatttttg ttaatccgct atatcgattt gaatttttca
       61 gaaaatgaag cggacgtttg ggcggaggca acttgtttga taagatagca atatttcaaa
      121 acggagaaag atcatgcctt atgtcaatat taaagtaacc ggcggcaagg aagcaccgac
      181 tgccgcgcaa aaagcggaac tgatcggcgg cgtaaccgaa ttgctggcac gcgtgctggg
      241 caaaaatccc gaaacaacgg ttgtcgtgat tgatgaagtg gataccgata actggggaat
      301 aggcggcaaa agcgtcagcg aacggcgcaa agagggcagg taaaaagcct gaaaatctcg
      361 gtttgatgct ttaaattccg cgtgaaaaag agtacattcc cacccattgc ccaaaattta
      421 cggaacacat catggataaa tttcccaagt ctgcaaagct cgatcacgtc tgttacgaca
      481 tacgcgggcc ggttcacaaa aaagcccttc agttggaaga ggagggcaat aaaatcctta
      541 aactcaatat cggcaaccct gcgccgttcg gctttgaagc ccctgatgaa atcttggtcg
      601 atgtcatccg caacctgccg acttcgcaag gctattgcga ttctaaaggg ctgtattccg
      661 cccgcaaagc cattgttcac tactatcaga ccaaaggttt gcgcgatatt acggttgatg
      721 atgtctatat cggcaacggc gtgtccgagc tgattacgat gtctatgcag gcattgctca
      781 acgacggcga cgaaatcctg attcccgcgc ccgactaccc tttgtggacg gcggcggcaa
      841 cgcttgcggg cggtacggta cgccattatc tgtgcgacga agaaaacggc tggttcccca
      901 accttgccga tatggaagcc aaaatcacgc ccaaaaccaa agccatcgtc gtcatcaatc
      961 ccaataatcc gacaggcgcg gtgtacagca gggaaatcct gttggaaatc gccgaactgg
     1021 cgcgcaagca cggtttgatt attttcgccg acgagattta cgataaaatc ctttatgacg
     1081 gcgcggttca ccaccacatc gccgcgcttg cccccgacct tttgacggta acgttcaacg
     1141 gtttgtccaa atcctaccgc gtcgccggct tccgccaagg ctggatggtg ctcaacgggc
     1201 cgaaacatca cgcaaaaggt tacatcgagg gtttggatat gctctcgtct atgcgcctgt
     1261 gtgccaatac gccgatgcag cacgcgattc agacggcatt gggcggctat cagagcatca
     1321 acgaatttat tttgcccggc gggcggcttt tggaacagcg caacagggcg tgggaactgg
     1381 tcagccaaat ccccggcgtg tcctgcgtca aaccgatggg cgcgatgtat atgttcccaa
     1441 aaatcgatac cgaaatgtac cgtatccacg atgacatgaa attcgtttac gatttgctgg
     1501 tgcgcgaaaa agtcttgctg gtacagggaa cggggtttaa ttggattaaa cctgaccatt
     1561 tccgcattgt cacgctgcct tacgtccatc agattgaaga ggcgatgggc aggttggcaa
     1621 gattcctgca aacctaccgc caataagggg acggtttgtc tgccgaggat aaaaaatgcc
     1681 gtctgaaacg gagattcccg tttcagacgg cattttcaac agcaggaacg aatcaggcaa
     1741 atttcagtct gtcgccgtcg gcttctaccc tgatttcgct ttcgggcgca tagtttccgg
     1801 caagcagggc ttttgccagc gggttttcga tttccgactg gatggcgcgt ttgagcggac
     1861 gtgcgccgta aatcgggtcg aaaccggctt tggcgatgat gtccagtgcg gcatcggaaa
     1921 cagtcaggcg caggttttgt ttttccaaac gtttttccaa acctttgagc tggattttcg
     1981 caatgctgcg gatgtttgcc tgatccaaac cgtggaacac gaccacttcg tcgatgcggt
     2041 tgatcatttc aggacggaaa tgttctttca catcctccat cacaacttct tttaccgctt
     2101 cgtaatcctg aatgctcatt tgttggatgt gctggctgcc gatgttggaa gtcatcacaa
     2161 tcacggtatt tttaaaatct accgtcctgc cttgcccgtc ggtcaaacga ccgtcatcca
     2221 atacttgcag caggatgttg aacacatcgg gatgggcttt ttccacttcg tccagcagaa
     2281 tcacgctgta cggtttgcgg cgcacttgtt cggtcaggta gccgccttct tcgtagccga
     2341 catagcccgg aggcgcaccg atcaggcgtg caacggcgtg tttttccata tattcggaca
     2401 tatcgatgcg aatcagatga tcttcgctgt cgaacagaaa gcctgccagg gctttacaca
     2461 actcggtttt acccacgccg gtcgggccca agaacaggaa gctgccgtaa ggcttgttcg
     2521 gatcggcaag accggagcgg ctgcggcgga tagcgtcgga cacggcacgc acggcttcgt
     2581 cctgtccgac cacgcggcgg tgcaatactt cttccatttt cagcagtttg tcgcgttcgc
     2641 cttccatcat tttggatacg ggaatgccgg tcatacggga aaccacctct gcgatttcct
     2701 ctgcgccgac attattacgc aagagtttgt ttgccggttt tgtgctgtcc gtatctgccc
     2761 gttcggcggc tgcacgctgt ttttccaaat gctccaaatc ttcatacatc aattttgaag
     2821 ccagtgccaa atcgccttgc cgttttgcct gttcgatttt aattttgact tcgtcaattt
     2881 gtttcttaat attagcagca ccgtctgaaa ttgctttttc ggctttccag atttcgtcta
     2941 aatcggcgta ttctttttgc agaccgttga tttcctcgtc tatcagttcc aaacgttttt
     3001 tgctggcatc gtctttttct ttttcaacgt gcgccttttc catccgaagc tgaattagac
     3061 ggcggtcgat tttgtccatt gcttccggct tggtttcttt ttccatcttg acacggctgg
     3121 cggcttcgtc aatcaaatca atcgctttat caggcaggaa gcggtcggta atgtagcggt
     3181 cgctcaactc cgctgcggca acgatagcag ggtcggtaat atcgataccg tggtggattt
     3241 cataacgctc ctgtaaaccg cgcaagatag cgatggtgtc ttctacactt ggctcgccaa
     3301 ccaatacttt ttggaagcgg cgttcgagtg ccgcgtcttt ttcgatgtat tggcggtatt
     3361 cgtccaaggt ggtcgcgccg atacagtgca attcgccacg tgccaaagcc ggtttcagca
     3421 tattgcccgc atccatcgca ccgtcggttt tgcctgcgcc gaccaaagta tggatttcgt
     3481 cgatgaaaat cagagtgttg ccgtcgtctt tcgccaagtc gttcaacacg cctttcaagc
     3541 gttcttcaaa ttcgccgcgg tatttcgcgc cggcaatcaa tgccgccaag tccaaaacca
     3601 gtaggcgttt gttgcgcagg gattcgggta cttcgccgtt gacgatgcgt tgtgccaaac
     3661 cttcgacgat ggcggtttta cccacgcccg gttcgccaat cagcacaggg ttgtttttgg
     3721 tacggcgttg caatacctga atcgcacggc ggatttcgtc gtcacggccg ataacggggt
     3781 cgagcttacc gtctcgggcg cgttgggtca gatcgagcgt atattttttc aaagcatcgc
     3841 gttggtcttc ggcattggca tcgttcacgt tttgtcctcc tcgtaccgcg tcaatcgcgg
     3901 cattgatgtt ttgttcggtc gcgccggctt ctttcaaaat tttgccggcc gcatcgttct
     3961 gctgcaccaa ggcaagcagg aaaagttcac tggcgatata ggcatcgccg cgtttggttg
     4021 cggctttgtc catcaggttc aacaccgcct gcaattcgcg gctgggcaga atgtcgccgc
     4081 cctggccgga cactttcggc aggctgttca aatgctgctg caaacgctgt ttcacctgcg
     4141 gcacgttcac gcccgcatga gccaagagcg cggcagcacc gctgttttgg tcgtcaagca
     4201 gggctttcag cacaaagccc gcttccagac agctgccgtc cgcagccaac gccaaactct
     4261 gagcttctgc aagggcctgt tggaatttgg cggttaattt gtcgtatcgc atttttgttt
     4321 ccttttcaaa atgtccgctg tcgaagccta tatgtgcata attgtggata actcaagttc
     4381 tgttttctgt ttttctatat ttaattcgat atatcattga atttaaagta tataaaaatg
     4441 tataataatg tgtataacta tatcttctta atatggaaaa gtctgttgtc ggctggatgt
     4501 aggtggcaaa tcgggtataa tcggcgcatc tttttccctt tcagacggca ttgatgccgc
     4561 aaggacattt ttatgagcaa aaaacgagtt ctgaccggcg taaccaccac cggcatcccg
     4621 catctgggca actacgtcgg tgctatccgc cccgccgtcc gcgcggcgca aaaccctgat
     4681 accgaatcct tcctcttcct cgccgattac cacggtatca tcaaatgcca cgagccggag
     4741 atgattcacc aatccaccca agccgttgcc gccacttggc ttgcctgcgg actcgacccc
     4801 gagcgcacca ccttctaccg ccaaagcgac attcccgaag tgatggaatt gaactggatt
     4861 ctgacctgca tcaccgccaa gggtttgatg aaccgcgccc acgcctacaa agccgccgtg
     4921 caggcaaatg cggaaaacgg gcaggaagac cccgatttcg gcgtggaaat gggtttgttc
     4981 agttatccga ttctgatgac tgccgatatt ctgatgttca acgccaacga agtccccgtc
     5041 gggcgcgacc aaatccaaca cgtcgaaatg gcgcgcgaca tcgccggccg cttcaatcac
     5101 cgcttccgag aactcttcac cctgcccgaa gtgaaaatcg acgaaaacgt cgaactcttg
     5161 gtcggtttgg acggacgcaa aatgtccaaa tcctacggca acaccattcc gctttgggaa
     5221 aacgacaaaa aaacccaaaa atcggtcaac aaaatcatca ccaatatgaa agagccgggc
     5281 gagccgaaac agcccgacga aagcccattg tttgaaatct acaaagcctt ctccacgccg
     5341 tctgaaacgg tggaatttac gaaaatgctt gccgacggcc tggcttgggg cgaggcaaaa
     5401 aaacttttgg cggcgaaaat caacgccgaa ctcgccgaac cgcgcgaacg ctacaacgag
     5461 ctgaccgccg acccttcgca aatcgaagag attttacagg caggtgcgca aaaagcgcgt
     5521 aaagaagcac gcgaattgtt ggacaaagta cgcgatgcgg tcggcatccg cccgttgaaa
     5581 taaacccgat gccgtccgaa cctcttaaag gcttgccgga acaaaacgtc gtccggctga
     5641 ccggcaagca tcccaacgac ttggaagccg tcagcggcaa atgcatggaa accgacgata
     5701 agggcggtcc ggtaggctgg gcggaaaacg gcgtgtgcca taccttgttt gccaaactgg
     5761 tgggcaatat cgccgaagac ggcggcaaac tgacggatta cctgatttcg cattccgccc
     5821 tgcaacccta tcaggcaggc aaaagcggct atgccgccgt gcagaacgga cgctatgtgc
     5881 tggaaatcga cagcgagggg gcgttttatt tccgccgccg ccattattga ggtattcgga
     5941 catcccggaa tatatttggg tttttcaaac cttgcgaaaa aaagtccgca ccgcagggaa
     6001 actcaaaagg agggggatat gtgttacaat tttccgaact gttttcataa aatagttttc
     6061 ggacgtgttt caatatggca ttgatgccgc cttattgttc gagaaaaaaa cctttatatt
     6121 taaatataat gggttttaac taaacgggaa accgttttct ctccggccga tgggcaaaat
     6181 cagccgattg atggaacacg gttcgatttt taagcaaaac cttaatcaac aactcaagaa
     6241 ggataagaca tgaaaaaatt attgattgcc gcaatgatgg cggctgcctt ggcagcttgt
     6301 tcgcaagaag ccaaacagga ggttaaggaa gcggttcaag ccgttgagtc cgatgttaaa
     6361 gacactgcgg cttctgccgc cgagtctgcc gcttctgccg tcgaagaagc gaaagaccaa
     6421 gtcaaagatg ctgcggctga tgcaaaggca agtgccgagg aagctgtaac tgaagccaaa
     6481 gaagctgtaa ctgaagccaa agaagctgta actgaagcca aagaagctgt aactgaagca
     6541 gctaaagata ctttgaacaa agctgccgac gcgactcagg aagcggcaga caaaatgaaa
     6601 gatgccgcca aataatttgt tgccttggca aaaatgatgg gatgccgcct gccggcaacc
     6661 caaacgaaac cgcctgaaga ttttcaggcg gtttttgagt tgtatgcccc ttgtttttta
     6721 cgcctgcatg accgttccga gcagatgtgc cgcgcatatc gggatgttgc ggacaggcaa
     6781 atgccgtctg gacagggttt ggacggcatt tgttccgtaa ggttcagacg acctcgactt
     6841 taaacggcat attgattttt tgccattgga cgctttcata ttccaatgcg tcggcaatca
     6901 ggggatggcg ttccagccat tccctgtcaa tacgcaggat gaagccgcag ctttccgtat
     6961 ccgtgcgcaa ctgcatattt ttcgggaaag acaggtcttg gcgcgaacgg cagaacaggg
     7021 cagcaaggcg cagggacaaa acggcatacc acaacatttc gttggtgccg atgatgccgc
     7081 tcattttttt catatcgccg cgatgaccga tgaccagttg ggcaagtatg gtctgttctt
     7141 tgcgtgagaa acccggcata tcggcgtttt cgaggatgta ggcggaatgc ttgtgatagc
     7201 cggtgtgggc gatgtccaaa ccgatttcgt gcagcgcggc ggcgcgtccg agatactgtt
     7261 gccacaaggc aagctcttga actgtaacgt ttttagcgtg gcagaggctg tccataaagg
     7321 tttgcgcggt ctcggcggtg cgtttcgcct gattgaggct gacgtggtag cggtgttgga
     7381 actcggcaac cgtttgtccg cgcatatctt cgtttaaacc gcgcccgatc aaatcgtaaa
     7441 acacgccgtc gcgcagggcg gcttcggtta cggtcatcct gtcgagtttc atttcctcaa
     7501 acgccgccat catcacggca agtccgccgg caaaaacttc gatgcgttcc ggtttcaggt
     7561 tttcaaattt ggcttttttg accgaaccgg cttcgatgat gcgttcggcg agggcgcgca
     7621 tgcctttgta ggtaatgtcc gcctcttggg gcatttcggc ggcaagcacg tcgcggatgg
     7681 attttgccga acccgatgtg ccgacggcga aatcccaacc ttcgcgcctc atatttttgc
     7741 tgatgcgctg gatttcgttg cgggcggcgg aaatggcaga ttggaagtct ttggcggtga
     7801 ttttgttttg gaagaagcgc aggctgtagg ttacgcagcc caagggcagg ctttcagtaa
     7861 tgtcgggatt cagcgtcgag ccgatgacaa attctgtcga accgccgccg atgtcgataa
     7921 ccagcatttt gccgccgtcc ggggggaggg tgtggatcac gccggtataa atcagccgcg
     7981 cctcttcgcg cccggcgatg atttcgatgg ggaaacccag tgccgcttcg gctttgggaa
     8041 ggaaatctgc gatgtttttg gcgacgcgga atgtattggt tgccacggcg cgtacctgtt
     8101 cagggcggaa gccgcgcagg cgttcgccga attttgccag acagtccaaa gcctgttctt
     8161 gggaagcggc actcaggttt ttctgttcgt ccagtccggc ggcgaagcgc accatctgtt
     8221 tgaacgaatc gatgactttt aattgtccgt tgttgttttc gcaaatctgg aggcggaaac
     8281 tgttggaacc caaatcgacg gaggcgagga tgtttgcggg ggtggtggtc atggcggata
     8341 tcggtggggg aaaaacgcaa tgttactctg acggcgcagg cgttgacaat aaatgatgcg
     8401 gcggtttttg attctgtcca cggatgttgc cgacggcatt ttttgcgctt atttgaaatc
     8461 cttttccacg ctcatgaaaa tctgcgtgtt tttgcgtgtg taaaaacttt tcatattgct
     8521 gtcgattttc agatagcgga aattgagttg cggcgtaaag cccttccaag agattttgtc
     8581 gtgccaaaac gacaggtttg cctgatattc gtggtctttg cgcgggaagc ggtacacaat
     8641 ggtcccgggc gcgtcaaacg tcctgcgtgt atagcgcagg tttgcccgca gacccaagcc
     8701 gccgtcgaac gttttgaccg cgccgatacg caaacccttg cggacggaag cctgttccgc
     8761 ctccttcgtc aagtcgtgcg accagtccgc cccgccgtaa agcagccagt ctttcggcgc
     8821 ggaatacatc agcgtcgcgc ccgccagcgg catatggctg tcgtatcggg cggcggtgcg
     8881 gtcttcctga taatgcttcc acatattgcc cgcgtttagt gtcaaccgcc agcgttcgct
     8941 caagtgttgg gagaaatcgg cattaaagcc gccgacgaaa ttgtatcggc tgccgcctaa
     9001 gaggttttgc ccgacaaacg gcacgatgcc gaacgagcgc gttaccgaac gatttttata
     9061 gccgagcgac aggtgcaggc actgttcgct gaaatctttg ttatcccaat aatgcacgcc
     9121 gccgccgctg atgccgccgt agaggaaatg atgcccgccc gcattgattt cgcgcgacac
     9181 gcctaagccc tagcgcaaac cgtgtgcctt ttgcggcagg ctgtcggcgg ttttcgtcca
     9241 gcttctgccc gcaaattcaa tcgttttttt ggacgaagcg ttgtttatag cggattaaca
     9301 aaaatcagga caaggcgacg aagcctcaga cagtacagat agtacggaac cgattcactc
     9361 ggtgcttcag caccttagag aatcgttctc ttcgagctaa ggcgagacaa cgccgtac
//
LOCUS       DAWFAD010000071         9372 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWFAD010000071 DAWFAD010000000
VERSION     DAWFAD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.071"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            40..645
                     /locus_tag="WGO49_001255"
     CDS             40..645
                     /locus_tag="WGO49_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ0306365.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QLSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            708..1070
                     /gene="ssrA"
                     /locus_tag="WGO49_001256"
     tmRNA           708..1070
                     /gene="ssrA"
                     /locus_tag="WGO49_001256"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1387..2136)
                     /locus_tag="WGO49_001257"
     CDS             complement(1387..2136)
                     /locus_tag="WGO49_001257"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0306366.1"
                     /translation="MPSVELLLEIVGLPRGTFYYQLAVQSAEDKYADLKRHIHDIYRR
                     HKGRYGYRRIAAAIRHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEI
                     LKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWF
                     KFNPLYNNDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(2141..2551)
                     /locus_tag="WGO49_001258"
     CDS             complement(2141..2551)
                     /locus_tag="WGO49_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ0306367.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            complement(2548..2778)
                     /locus_tag="WGO49_001259"
     CDS             complement(2548..2778)
                     /locus_tag="WGO49_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306368.1"
                     /translation="MVSNPATGKISHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGA
                     MVGGYALIKRGIAAVPQLAEVKKSANQEGGRQ"
     gene            complement(2768..2977)
                     /locus_tag="WGO49_001260"
     CDS             complement(2768..2977)
                     /locus_tag="WGO49_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306369.1"
                     /translation="MSKKSILALMAAAMQPDFSHSDLGIRYAMPTQGCWTQAHRKSGV
                     AAAKRAAKKSVTNNRLFPMAGRLGL"
     gene            complement(2988..3491)
                     /locus_tag="WGO49_001261"
     CDS             complement(2988..3491)
                     /locus_tag="WGO49_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ0306370.1"
                     /translation="MSDEFNRFIERVLSHEGGYVNHPQDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAISIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VPDDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     AAADNAD"
     gene            complement(3581..4063)
                     /locus_tag="WGO49_001262"
     CDS             complement(3581..4063)
                     /locus_tag="WGO49_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306371.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            complement(4060..4971)
                     /locus_tag="WGO49_001263"
     CDS             complement(4060..4971)
                     /locus_tag="WGO49_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306372.1"
                     /translation="MFDLEVALRRVLSAAPTVQDTQIEQYNQLTQIMHRTLDPKDVKG
                     NSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPPSGYSFLRKEAFLYIVGHHV
                     IFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFKAVGNCDKLSLIQQHGVKSI
                     LLDVSAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSDEELEAQSEIHINLEVLLNG
                     NSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVIQPSDVKLSKSVRIGRYDEA
                     NSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            complement(5261..5506)
                     /locus_tag="WGO49_001264"
     CDS             complement(5261..5506)
                     /locus_tag="WGO49_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306373.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            complement(5535..5912)
                     /locus_tag="WGO49_001265"
     CDS             complement(5535..5912)
                     /locus_tag="WGO49_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0306374.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            6026..6205
                     /locus_tag="WGO49_001266"
     CDS             6026..6205
                     /locus_tag="WGO49_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0306375.1"
                     /translation="MYGYTVEKLKENFAQNGQTLAQWARENGFKPRDVYLVVGGQRKG
                     NYGKGHEIAKKLGLK"
     gene            6217..6507
                     /locus_tag="WGO49_001267"
     CDS             6217..6507
                     /locus_tag="WGO49_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0306376.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            6552..7427
                     /locus_tag="WGO49_001268"
     CDS             6552..7427
                     /locus_tag="WGO49_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ0306377.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(7449..7760)
                     /locus_tag="WGO49_001269"
     CDS             complement(7449..7760)
                     /locus_tag="WGO49_001269"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306378.1"
                     /translation="MTSPVSPSIRAASIFPLARSISTACRTDINRLPLRLIASTKTAE
                     INLASSGLSSVLPASLRRLRVGLMLTASSLRYSVARLMPNSCACCLRYAERAPRPIAS
                     A"
     gene            7739..8290
                     /locus_tag="WGO49_001270"
                     /pseudo
     CDS             7739..8290
                     /locus_tag="WGO49_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            8384..8647
                     /locus_tag="WGO49_001271"
     CDS             8384..8647
                     /locus_tag="WGO49_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306379.1"
                     /translation="MADILPSDKQRQPPKTKGNPMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMIAATGLVTAIAAVLALIFKFA"
     gene            8684..8965
                     /locus_tag="WGO49_001272"
     CDS             8684..8965
                     /locus_tag="WGO49_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306380.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDGEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            8977..9189
                     /locus_tag="WGO49_001273"
     CDS             8977..9189
                     /locus_tag="WGO49_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0306381.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWIEKTNAV"
BASE COUNT         2572 a         2398 c         2146 g         2256 t
ORIGIN      
        1 ccgcccgaaa cagccatcgg acaaataaca ggaaaaaata tgaataccga cttccaaaac
       61 atcctcaaac aactgggcaa acaagcccaa aaagaagcgg cagaaaaaca ggcagccgaa
      121 aaaaacaagc aaaaacaaga acaggatttc gatttttcac aagcagtcgg acaactctcc
      181 ccattaaaaa accggcagca atattatgcg ccgcccgaca aaacccccat caaagtccgt
      241 cccaaagaca accgcgccga cgaagaaaac tacttctaca tcggcagcac atacaacgac
      301 ccgcccgcca gcttcagcaa aaacggacag ggaaaaaacg acatccaacg cctgaaaaac
      361 ggatactatc ccgtcgttac cgatgtcgac ctgcacggct acacacagga agaagcccaa
      421 aaagtcctca acgaattcat cgcattcacc caaaaacgcg gcgtatgcgg cgaaatcatc
      481 cacggcagcg gattaggttc caaaggctac aaacccgttc tgaaaaatat gacccgaaac
      541 tggctgatgc aacaccccga cgtactcgcc tacgccgaac cccgggaagg caacgacggc
      601 tgcgtccgca tcctgctcaa acgcaaatta aggcagcaag actgacaaat ttctccgaca
      661 gacttggcag caccgcacaa tatccgtata atttgcaaac catttttggg ggcgaccttg
      721 gtttcgacgg gggttgcgaa gcagatgcgg gcataccggg gtctcagatt cccgtaaaac
      781 actgaattca aatagtcgca aacgacgaaa cttacgcttt agccgcttaa ggctagccgt
      841 tgcagcagtc ggtcaatggg ctgtgtggcg aaagccaccg caacgtcatc ttacattgac
      901 tggtttccta ccgggttact tggcaggaaa taagacttaa ggtaactggt ttccaaaagg
      961 cctgttggtc ggcatgatgg aaataagatt ttcaaataga cacaactaag tatgtagaac
     1021 gctttgtaga ggactttcgg acgggggttc gattcccccc gcctccacca taccctattt
     1081 tcagcagcat cctgcaacac aaaaaagcct tgaacttcaa cagtttcaag gcttttttgt
     1141 gttgcaggat gctggcgtat gcttgagttt tcgccattta tggggtaact tttggggtaa
     1201 cttttcacga attaccccaa aaaacgcctt aaaaatgcaa aagtcatcac gcattttttt
     1261 aaaaaggtta cccaaaactt aaatgtaatc ctttatttat aagcttgcag ctttccaaac
     1321 aagttacccc caaacggata aaaaaatgcc gaagccgaac tgaccccaaa aagtcggaca
     1381 gtttaatcaa gcggctttca gggactgaat tctgtactga acaaggctca gcccttttaa
     1441 tttcaacttg attctatcgt tgttgtatag tggattaaat ttaaaccagt acggcgttgc
     1501 ctcgccttgc cgtactattt gtactgtctg cggcttcatc gccttgtcct gatttaaatt
     1561 taatccacta taaaaaaaca tctgatattg ccaaccttga tccgaatgca gtatcggctt
     1621 ttcagacgat cccggtttct ccggcgcacc tttcagtatc tcgccggcca aatcgaaagt
     1681 cgggcggatt tgaatacggt aactgacgat ttccccgtta aacaaatcca taatcggaga
     1741 aaggtatatc ttttctccgc ctacattgaa ctcggtaacg tccgttaccc atttctcatt
     1801 cggcttttct gcatggaaac agcgtcgcag gatattcggc gcaattttgc cgacttctcc
     1861 tttgaacgag cggtatttgc gccgccgtat cactgccttc agccccgtct tcgccatcag
     1921 acggctgact ttcttgtgat tgaccggtgt tcctgcgtga cggatggctg ccgcaatcct
     1981 ccggtagccg tatcttccct tatgtcgtcg ataaatatca tggatatgcc gtttcaaatc
     2041 ggcatatttg tcttctgccg attggacggc caattggtaa tagaaggtgc cgcgtggcag
     2101 tccgacaatc tccaacagca gttcaacgga tggcattgcc ttaaccctgc gacgagttgc
     2161 gttctttctg ccgcacttct ttcccatgga ttaaggcatc gggctttttt agggcagcca
     2221 tttccgctgt aaggcaagcc aattccgcaa gcagttcttc cttggttttc agatagccgg
     2281 cttttttcgt ttgcggcgga tactgttttt tcacgggctt tcttccttgt ttttgggaaa
     2341 agtcgtacca ccgctgcctt tcgtcctgcg cttttttaag ctgcaaggca tattgttccg
     2401 ccgcaaccat agaagaatgc cgataggtgg caatcagggc ggtttgttcc gcttcggcct
     2461 tgtcggcggc gtatcggtaa ccgcacgtcc acacaagcaa aagcacaaat gcccaaagca
     2521 gccgtttttt ggctcggaca aattcaatca ttgccgcccc ccttcctgat ttgcggattt
     2581 tttgacttcc gccaactgcg gtacggcggc gatgccgcgt ttgattaatg catacccgcc
     2641 gaccatcgcg ccgtatgccc accaaagcca ttcgggcgcg tccgccgcct gcacgaactt
     2701 ccaagtcatg gcggctgcgg cgacgtttgc ccaaagtttg gtatggctga ttttccctgt
     2761 ggccggatta gagaccaagc cgcccagcca tcggaaaaag gcggttattt gtgacgcttt
     2821 ttttggctgc gcgtttcgcg gcggctaccc cgctcttgcg gtgggcttgc gtccaacatc
     2881 cctgagtcgg catggcgtag cgaatgccta ggtcgctgtg gctgaaatcg ggctgcatgg
     2941 ctgcggccat cagggcgaga atggactttt ttgacatggt ttgctcctca atcggcattg
     3001 tctgcggcgg cgtgaatcag gttttgcgcc acacggcgta cccagccctt gccgaaagag
     3061 gtgaacgtac ccagcttggt ataaaagacc agacgctcgg cgttgaaccg caataaaagg
     3121 tcgttttcgg gaagggaatt gatggctttg aggctgattt cgccgatgat tccatcgtcc
     3181 ggcacgcctg cggcgcgttg gagcatacgg gcggcattgc cgtaaccgtg gttgacgcag
     3241 gcatcaaaaa attggaacgc gaccgcttcc ggcatttggt cggcgcggta acgctcccaa
     3301 aacgctttac ggtaaatgct gattgcctgt tcacgcgtca tggcacgcat ggagccgttg
     3361 tagccgtttg cctgggcggt gcgcttagtg atgccccaat tggtttcgcc gccggggtct
     3421 tgagggtggt tgacgtaacc gccctcgtgg gagaggactc gttcgataaa tcggttgaat
     3481 tcgtctgaca tggaaaaatc cctgtattga attaaaaatc aatacaggga ttttagaaaa
     3541 ggtcgtctga atgggatttt aaagggggtt aaaactcgtt ctagattttg ggtttcttct
     3601 tttccattaa ttttttaaac tcttcttttc ggcttttaat taaattccca ggtaagaata
     3661 cagaataaaa aatggaaata caggctaaaa ataaatagct ccgttcaagc caaaaaatgg
     3721 ctggtccatt tttgtattct tctttatgag ccaacaagat gacaataaaa acacaaacta
     3781 atacggcaag atatgtgtaa aaaagtaagg aatgttttat ataccgttgt tcttgtacgc
     3841 cttcacgaat ttgaatttgt cgccaatttg catcttcatc aaacctcata tcactaaaca
     3901 atgtcataat ggcaattaaa aaaccggcta agattgcaaa tacgttaata attaaatcaa
     3961 cggccttctc gttcccatga acaaaaggtt gccctttcca tgctaaaaat gctgatatac
     4021 aagcaatcaa caaaaaacgt agtaagggca gatatttttt cattgttcag ttaagtttct
     4081 gctttggagt tctaggaaat attcactgat tgctgtaaat gccgagtcag ggagtaacga
     4141 gttggcttca tcgtatcgtc ctattctaac agatttagac agttttacat cagagggctg
     4201 aatcacttca ccttgttgcg tggtaatgga aaatccttga ttaaccgttt catcatcaat
     4261 aatttcctct gcctgctcct gcatcagcga ttgcgcttca atactggctc tcgaattacc
     4321 gtttagcaag acttctaaat tgatatgaat ttcggattgt gcttccaatt cctcatcact
     4381 caattcgctt gtaaatacac taccaacttt ccctaatgct ttagcaatgg ttgaacggct
     4441 atcttgatac agtctgtttc tggataattg atatgccgaa acatctaaaa gaatactttt
     4501 taccccatgt tgttgtatca atgacaactt gtcgcaatta cctactgctt tgaattcaat
     4561 attaaattga acaatatccg gattgttctc tctcaattta ttgctaagta aacttaaata
     4621 agaagcaacc gttgatgctg acaaaaaacc atgtccgcaa aaaattacat ggtgtccaac
     4681 aatatacaaa aaagcctctt tacgcaaaaa ggaatatcca cttggaggag cttgtgttcc
     4741 accatgatca tcttgctgta caggtctatt gctcatagtg cggatatgtt catcttttgt
     4801 accgctgcca atgtgtatca gtaatccacc aattgaattg ccttttacat ctttaggatc
     4861 caaagttcta tgcatgattt gggtaagctg attgtattgt tcaatttgtg tatcttgaac
     4921 agtaggagca gctgacaaaa cccttcttaa tgctacctct aaatcgaaca agctatggtt
     4981 ttgctgcaca cgtaaataat gaatagtttt agtagttttt ttgccatttg tacagtcctt
     5041 tctcttttaa tatctgtgtc gaaaaaataa attcagacgg catcaattca aatctttcaa
     5101 cacctgaacc acctgcccga tgacctgtat gtcggggtag tttttaatgc gttttaaaat
     5161 gcacggccgc ttcaattgct taatatccgt ttcagaacac cggagcagcc cgactgtcgg
     5221 agtgtcctaa atgccgtctg aaaatttcag acggcatttt ttatttttca aatagctctt
     5281 tgccgattgc ttggtaatct acttcttttt cagttggatt taccgtgtct gcataggctt
     5341 ctaccaaaga aaacacacca gcttggctta gataatgcac ttttaccgcc ttagctaatg
     5401 ccgaagccaa aggttcggga atttcgatat tgttctgggt ggctgcctct ttaacgattt
     5461 cttccgctcg ctcaccccat ttgatttgtt gttcaaagtc aaacataaga ctgtctccat
     5521 tttggattta aaaatcacgc tgttttatgc gtggctgaaa cttgagtatt ttcggctgct
     5581 tttttctctg cgccttttgc cacactgaga atgacaaatt tgccgttatc gctggcttgg
     5641 cggtaatacc ctaataactc tgactcttcc ttactaattt cagaattttc agacggccta
     5701 attccctgtt cagcagcaaa taagctgctc acatctattc ccatattcaa aaaagagagc
     5761 aatacatctc cactaggcat aaatacgcca cgttcgtatt tgccccatgt ttcgcgctca
     5821 atacctgctt tttcagcagc ttgtgcttgg gtaagcccta aaaactttct tttttctttc
     5881 aaacgattac caaaaagaga tttagaaatc acaaaataat ccttgcaaaa gagattttaa
     5941 aatctcataa ttcacgcatt ggtaatgcac taattaattt ctagtgcata aatagttcac
     6001 tattttatca caatgggaaa gggctatcta tggctatact gttgaaaaac taaaagaaaa
     6061 ttttgcccaa aacggccaaa cgctggcgca gtgggcaaga gagaacggct ttaagccgcg
     6121 cgatgtgtat ctggtggtcg gcggacagcg caaaggcaat tacggcaagg ggcatgaaat
     6181 tgccaagaag ctgggactga aataaggagg atgcggatgg cagaaagtaa aagggtacaa
     6241 cggctattga gggtctttat cgcgcttgac gagcatccga ttatcggtct gagcaataag
     6301 gatttatcgg tcggactggg gctgacgcca tcgcaagtca gcagggatat tgatgatttg
     6361 gttgcctcgg gattggtcat caaacttgaa aacggcaact acgcctacgg catcaaaacc
     6421 ctgcaaatcg cagagcggtt caggcagcag catgaacggc tgcaaagcaa gattgccgaa
     6481 atcggcaagc gggtcgatgt ggattaacgg attttgaaag tttccgacgt cggaaacttt
     6541 ggagataaaa aatgaataca gaaattgaaa ttatcgcaac cgacaaagta agcaatcagg
     6601 cagcgatgcg cagtgtgctg gtgatggagc agtggggtaa cggcgagact tatagcgaag
     6661 agcgttgggt tgaacgcggt cgtcaagcag tacggcagac gatggaaggc atgttcgaac
     6721 tgggacgtgc gctgattatt ttaaaagagc atacggaaca cggacggttt atggaaattg
     6781 tgaaaagcca atttgggctg ggtatcgcag aaacatcacg tctgatgtca gccactcgcc
     6841 gctttgcgac cccgcaaatg cagaaagccg cgccgaagct gatggatttg ggcaagtcaa
     6901 agctgctgga actcttggtc gaagaagacg ttacgttggt gggacttgcc gaaggagaag
     6961 aagtcaacgg catgaccttt gacgatgtgg accgaatgac ggtgcgcgag ctgcgcgtcg
     7021 ccctgcgcga aagccgtgaa aacctcgccg ccaaagacga agtgatgaaa accaaaacag
     7081 ccaaaattga tgagctgacc gagaagctgg ctaagaagca aaccgttgtc agagagccga
     7141 aggcggaaga cgtgggcagt gagttggcga tgcagttaac aagccttgag gtcggtatcc
     7201 gcagtcaagt gagccgtttg aaagatttgt tcgaccaact taatgcgcac agtgaggcac
     7261 acgagatcag ccatcaggca aagatggtcg gcacgctcaa tcaaattatt ttggactgcg
     7321 agcaactgcg cgaaagctat gccctgccga ctgaagcacc gacagacaat gtgccggaat
     7381 ggttgggcgg tgaaacggga gaaggcgatg aatccggcaa tgattgagcg tcttaaggca
     7441 gtcgagaatc aggcggaagc aatgggacgc ggcgcacgct ctgcatatct taagcagcag
     7501 gcgcaggaat tgggcatcag ccttgccacg ctataccgca agctggaggc ggtcagcatc
     7561 aagccgacgc gcaaacggcg cagcgatgcg ggcaagacgg agcttaaacc ggaagaagcc
     7621 aaattgattt cggcggtttt ggtggaggcg atgaggcgca acggcaagcg gttgatgtcg
     7681 gtgcggcagg cggtggaaat gctgcgcgcc aacggaaaaa tcgaggcggc gcggattgat
     7741 ggggaaaccg gggaagtcat cccgtccgtc cgcactgctt tccgcccgct ggcaagacat
     7801 cgacctcacc gcccggttat ggcacatccc cgccgccgac atgaagacca aacacccctt
     7861 taccgtccca ctgtcggact gggcggtaga aatactccga gagctgcaca cacagaccgg
     7921 cgacaacatc caccttttcc caggcatccg cccacggcgc aaacccgccc cccatcccga
     7981 ccacatcagc atcaaattcg cccacaacgc catccgacga ctgggctatg acggcagcac
     8041 ccccggcaaa tccaaacaca caatgcacgg attccgccac ctcttcacca acatcagcct
     8101 gaccgccgga aaagacaccc tcacaacaga cctcgccctc ggccacatca gccgcgccgc
     8161 cctccaacgc gccggcttct caagcctgca ccactacctg accgccgaca gctaccgcct
     8221 ccaagagcgg cgcgaactcg ccgaatggta cagccgccgc caccgccaag catacgaagc
     8281 cgccgccaaa accccgcaaa acaaaaaacc gcctgaaaaa aattcagacg gcatcctgcc
     8341 caaaacactt gcaataaaac ctaaaaggta ttaatgtaca cacatggcag acatcctgcc
     8401 atccgacaag cagcggcaac cgccaaaaac gaaaggaaat ccaatgaaca taatcgaaca
     8461 agaaatgaaa cactccagtc tgagaaaagt agatgccgaa atcgccaaaa tcattgcaga
     8521 cgcgcacaaa atcaacgccg aatccgtaaa aatagcccaa gagtcccgct ggtatcccat
     8581 gatagcggcc accgggctgg ttaccgccat cgccgccgtg ttggcactga tattcaaatt
     8641 tgcctgacca caagccccgc gaggggcttt atccctcaac aaaatgaaca gagccgcact
     8701 attcaaccgc taccccgaat ggattatcgg gcaagacggc gcaagccgtt tcattaccca
     8761 ttgccgctat ccccgcctga ttgccaaaat ccacaggcag acagacggcg aatgcccggg
     8821 cgggcattac cgccattccg aaaacggcat caccctttac gattttattt ttttcgacgg
     8881 caaaccggca gacgaagccc gatttgccgc cgtccttacc gaaacctgcc gcagggcagt
     8941 caaaaaaata tgcgaaacaa aataaaccgc aacgacatgg aactcggcta cacgccttac
     9001 aaccttcgca cccttcgcaa ccgctgcaaa ctgacacagg ccgagttggc gcaaatcgtc
     9061 ggggtgaagc actacataca ggtaggccgc tgggaagccg aacccgatac cgaaacccgt
     9121 cgggccgata tgccgctgga aaaatggcgg cagttcctcg attggataga aaaaacaaac
     9181 gccgtctgaa accttcagag tgcgaagcct gcccgccggg tacggcattt tttacccacc
     9241 cccaaaccta aaagccccac gccgcttgcc cccgacccgc cgccgcccca aaataaactc
     9301 actctgagac ctttgcaaaa ttcctttccc tcccgacagc cgaaacccaa acacaggttc
     9361 gccccaaata cc
//
LOCUS       DAWFAD010000072         9328 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWFAD010000072 DAWFAD010000000
VERSION     DAWFAD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.072"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1067..1735)
                     /locus_tag="WGO49_001274"
     CDS             complement(1067..1735)
                     /locus_tag="WGO49_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0306382.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLESGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLESYGKTYFYSDSKNDLPLLRLVDEPVAVNPDAELEEEAKEKGWPV
                     LNFK"
     gene            complement(1732..2400)
                     /gene="hda"
                     /locus_tag="WGO49_001275"
     CDS             complement(1732..2400)
                     /gene="hda"
                     /locus_tag="WGO49_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ0306383.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            complement(2505..3122)
                     /locus_tag="WGO49_001276"
     CDS             complement(2505..3122)
                     /locus_tag="WGO49_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0306384.1"
                     /translation="MVWPEYSPQENVALFRHNGEILLSGINIKLKSGDSLLIRGPSGC
                     GKTSLLCALAGLWPFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVCYPDIDKQHPE
                     LAEAMNTCRLGYLIDKLDKTADWQHKLSPGELQRVAFVRALLSKPKIVLLDEATAALD
                     EPAEAALYRALKQKLPDSIIISIGHRSTLNTFHNMHLNVGNVACD"
     gene            complement(3477..4376)
                     /locus_tag="WGO49_001277"
     CDS             complement(3477..4376)
                     /locus_tag="WGO49_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEZ0306385.1"
                     /translation="MPALTVTWMIYTKYLVYQIYLVIFCGVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            4420..4671
                     /locus_tag="WGO49_001278"
     CDS             4420..4671
                     /locus_tag="WGO49_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0306386.1"
                     /translation="MQANEIYKSLEEKNISARMLASALGVTNQSVSEVIRNGRGSKRI
                     AEAIAKVIQKDLVEVFPHYKKTDCRDKKIAELKEMLGVG"
     gene            4673..6652
                     /locus_tag="WGO49_001279"
     CDS             4673..6652
                     /locus_tag="WGO49_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0306387.1"
                     /translation="MEYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKTLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     DARCAVVAAVLGIKYQYDCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSRSAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIAGNPQGRGI
                     IERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPGWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAPDMLSA
                     QELATMFMPQEVRKVQRGWLDLFNNSYFSVELAEYHKDEVRVSYDLSDASAVNVFDMD
                     GKFITKAQANGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALEQAAVW
                     DELGHLGGNVIEAEYAVLPKTGTDDGIFLFEADRS"
     gene            6688..7602
                     /locus_tag="WGO49_001280"
     CDS             6688..7602
                     /locus_tag="WGO49_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0306388.1"
                     /translation="MKQINQALQQKLAEFKEKSGMSQTQLARGIGTSPASVSMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKAKREELVLGFVSTKTTRRIVEAMRNAHEGGEIS
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGHLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKYG
                     ELVQLHSRVAGALNLGDTMPDAELEQIARATLPEADDETIAELVKHSNGNTRRMSKLM
                     RGSVRVANKNGIRMQAGIVKKYSTLIIR"
     gene            7692..7958
                     /locus_tag="WGO49_001281"
     CDS             7692..7958
                     /locus_tag="WGO49_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306389.1"
                     /translation="MQVLKKVDWKMFVARSFWQCVPVGLTAGVWCFVAGMALHSCAKE
                     PEPVAKEPTKVEAMERQADLEVKVWEGQYESMSVEEKMKGIVYE"
     gene            8067..8240
                     /locus_tag="WGO49_001282"
     CDS             8067..8240
                     /locus_tag="WGO49_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306390.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            8233..8400
                     /locus_tag="WGO49_001283"
     CDS             8233..8400
                     /locus_tag="WGO49_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306391.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            8464..8691
                     /locus_tag="WGO49_001284"
     CDS             8464..8691
                     /locus_tag="WGO49_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306392.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            8706..9224
                     /locus_tag="WGO49_001285"
     CDS             8706..9224
                     /locus_tag="WGO49_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ0306393.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDTILNQKECFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
BASE COUNT         2483 a         1855 c         2611 g         2379 t
ORIGIN      
        1 gtcgccttgt cctgattttt gttaatccac tatacgtcct agattcccac tttcgtggga
       61 atgacgggat gtgggttttt gtgcggattt gaaccggtaa gggtggtgtg ggattggtgg
      121 tttgcttagg atcttttgga ttgtattttg tatatacatt tacttgttga taaaagataa
      181 aataaaatta gaaactaaaa gtgagaaaaa attaataata atagggatgt ataaatgtaa
      241 agactccgtt tcatagctaa ggttatctga atatatggaa aaaaagtaaa agtccataaa
      301 actaaaatat atagataatg ctaatgataa tagaattatc actttcctag agtaaggata
      361 atattttttt atcgtaaaaa aatataaaat aggataaata attgccatat agatagattt
      421 tgtaacaaaa ttaaaaataa ataataaaaa taatgtaata aagtatattt tttggctatg
      481 aaataaaatt gtacataatt gaacgagcag atcaaaaaat gaactacata taacaataaa
      541 taataacgta tttaccatac taaatttaat aggtctcatt atcatattta ataaccactt
      601 catagtatag tggattaaat ttaaaccagt acagcgttgc ctcgccttgc cgtactatct
      661 gtactgtctg cggctttgtc gccttgtcct gatttttgtt aatccactat aaatgcagag
      721 tgggtggaaa cactcacttt atggtttgct acgctctgct caattagcaa cccgataacc
      781 caatatggat aatagggtaa ttaatccaat ctaatttgtc agcatctgtt aatttattgc
      841 aaaataaagt attgaattat gtcgggtgca aatgacgaaa tataagtttc cgtgcggacg
      901 gatcaagatt cccactttcg tgggaatgac ggtggaaaga ttgttgtttt tcccgatgaa
      961 ttcctgtgtt ttttgttttt ccggataaat tcctgtggct ttgagttttt tggatttcag
     1021 cctcaatgcc gtctgaacgc cgaattgggc ttcagacggc attgcgtcat ttgaaattta
     1081 aaaccggcca gcctttttct ttggcttctt cttccagctc ggcatcgggg ttgacggcga
     1141 cgggttcgtc gacgaggcgc agcagcggca ggtcgttttt ggagtcgctg taaaaatagg
     1201 ttttgccgta gctttcgagc gtttcgccgc gttcggcgag ccattggttc aggcgggtga
     1261 ttttgccttc tttgaggctg ggcgtgccga tgtaattgcc ggtgtagcgg ccgtcggaac
     1321 cggattcgag ttgtgtgccg atgatgttgg taatgccgaa aaggtggcag acgggggtga
     1381 tgatgaactc gttggttgag gaaatcacaa gggtttcgtc gcctgccatt tggtggctct
     1441 gcactagcat acgctgcata ggcgagatgt gggggatgat gtattccgcc ataaattcgc
     1501 ggtgaaattc cgccagctct tctttgctgt aacgggcgag cggggcgagg tggaatttga
     1561 ggaatgcgtc gatgtcgagg cagccgtttt ggtagtcgcg gtagaatttt tcgttttgcg
     1621 cttcggtttc ggcagcgtca accaagcctt ttttgatgag gtattgcggc caggcgtggt
     1681 cggaatcggt gttgatgagg gtgttgtcga ggtcgaagat ggcgaggttt ttcattgggt
     1741 ttcctgttgt ttcaaaagct ggcgcaaaag cggcagggtg atgcgtttgc ccatcgtgac
     1801 ggcgtagttg tccagcgtgt cgagcatcat catcaggctg tccatatcgc gccgccagtg
     1861 tttgagcagg tattcgaaaa tttcggaatc aacggttact tggcgtgccg ccgccatact
     1921 ggcgagcgcg tcgatttttt cttggtcggt caggggtttg acttcgtaaa cgaggcagta
     1981 agccatacgc gtccgcaaat cttcgcggat gacgagctgc tggggcgtgt attccgaacc
     2041 gagcagcaaa aagcctttgc cgctgttgcg gaagcggttg aagatggaaa aaagcagggc
     2101 ttgttcttcg ttgcccagct tttcgacttg atcgacggcg aggtattccg cctcgaacgc
     2161 ggcatcggtc agcggcatgg aggcggcatc gatataggcg gcgtttttgc cggcttcgag
     2221 cgcctgtgcg acccacgcct gcaaaagatg gcttttgccc gcgccttctt cgccccaaac
     2281 ataaatgaat tgtccgtgtt tgtgtcggag gacatagacc agttccgcgt tttccgtgcc
     2341 gaggaatttg tcgaaactcg gatagtcgtg tgcggcaaag tcgaaaataa gctggttcac
     2401 ggttcggcat tccgaggggt ggtaaacggg tttattgtac gttgttttcg cgcgcctttc
     2461 caatttgaac gatgccgtct gaaaacggct tcagacggca tcgttcagtc gcaggcaacg
     2521 ttgccgacat tgaggtgcat attgtggaac gtgttgagcg tgcttcggtg tccgatgctg
     2581 atgatgatgc tgtcgggcag tttttgcttt aaggcgcggt agagtgcggc ttcggccggt
     2641 tcgtccaagg cggcggtggc ttcgtcgagc aggacgattt tgggcttgga aagcagggcg
     2701 cggacgaagg cgacgcgttg cagttcgcca ggggagagtt tgtgctgcca gtcggctgtt
     2761 ttgtccaatt tgtcaatcag ataacccaag cggcaggtgt tcatggcttc ggctaactcg
     2821 ggatgctgtt tgtcgatgtc cggataacaa accgcgtcgc gtaggctgcc ttgcgccgtg
     2881 tacgggcgtt gcggcaggaa gaggatgtct tgatgcggcg gacggctgac tttgccgctg
     2941 ctgccgaacg gccaaagccc agccagcgcg cacagcaacg aagttttgcc gcaaccgctc
     3001 gggccgcgta tcagcaggga atcgccgctt ttgagtttga tgttgatgcc gctcaacagg
     3061 atttcgccgt tgtggcggaa cagagcgacg ttttcctgtg gggaatactc cggccatacc
     3121 actcaacacg gcctatgatg gcgatgtcgt cttgggtatt actcaaatct atttcaaacg
     3181 gtgcgtaacg tggattttca gacgttacaa gcagtttgcc cggtatacgt tgcacacgtt
     3241 tgacaaagag gtcattgcct atacgcaaga catataggcc gtcgcgcggc tcggtttcgg
     3301 cgtggttgat tagaatgttt ataaaaggct ttaactagaa actgaacatg gaacttgtat
     3361 aatagttcca tgtttgaaaa aatcttttaa tatcatacat ctaaaaaaac aaagcatctg
     3421 aagaccataa gctaaacatg aaacttgaat ttaaaggcgg agtttcatgt ttaagcctat
     3481 tttttccgtt ttttagtcga tactgctgca tatgtttttt gcaaatcttc atcacttaat
     3541 tcagtcaggc tttctactga gaaatttttt tcgaggtaac ttttcaacca atcttcaagc
     3601 tcaagctgtt ttacatttaa tttgatataa gcgtatttct tttttcgcca gtcgggatcg
     3661 ttgttctttg atgttgctgt attgctcaac ctacccagcc attttctgag ataagtttct
     3721 gctttgtcgt aatcgctcaa tgcgattagt ttgtaacttg gaactttaca gtgagcattc
     3781 agactagccc aaattgcacg gattgatttt ggcgaccgtt tggcaatctc ttctgctgcg
     3841 gcaacttgtt taacaagttg ttgcagcttt gaagcttgct caagcgttat atgttccttg
     3901 ccgggttttg aatcgacacg ggttctgatg acgtgatttt gggtattaat ttgatgaaca
     3961 accgaaccag atgaggcgat aacagcgtta ctgacgttgc cgttaatctg attctcaacg
     4021 tttgatttgc caatagcgtc gtccaatttt ttattaaggt cgtctgaaac agcatttaaa
     4081 tttgccgaat gaatgcctgt aaagacaaat tgaatatcca cccctaattg gacagcagca
     4141 agtaaaaagt ccgcaggaat gttgacctgc ccgctttcat acagtctcaa actttctgca
     4201 ctacatccag tttgctcagc aaaattcttt cgagagtatc caagtcggct tctctcttca
     4261 actaaattca atgctaaatc aatgcgttcc ataaaaacac cgcaaaatat taccaaatat
     4321 atttggtata ccaaatattt tgtgtatatc atccaagtaa cagttaaggc aggcattaaa
     4381 gcctgtaagg taagtaatgg attctatcat ggaggcatta tgcaagcgaa cgaaatctat
     4441 aaatccttag aagaaaaaaa tatatcggca agaatgttgg cttcggcttt aggggtaact
     4501 aaccaatctg tttctgaggt cataagaaat ggacgaggaa gcaaaagaat tgcggaggca
     4561 atcgcgaaag tgattcaaaa agatttagtt gaagtttttc cgcattacaa gaaaacagat
     4621 tgtcgagaca aaaagatagc tgaattgaaa gagatgctcg gagtgggttg aaatggaata
     4681 tatggatctc aaaagcctgc ttaattttgg gttgccaaat atgccaagaa cagtaatagg
     4741 gctgagaaaa aaagcgcaaa gggatggttg gcaaacgaga aggcgtcaag gtaagggtgg
     4801 tggtgttgaa tatgccctcc ctgccgaaat ccgagcagcc atcatgaaac ggcagtcgga
     4861 cgagctggcg gagaagatgc cgaaaaccct gccccaagtc agaccgggga cggcgatgtc
     4921 ggctcaggca ctggctgaag cggccaagct gttgaacgag aaacaacggt cggtggcgga
     4981 tgcgcgatgt gcggtggtgg cggcggtgtt ggggattaaa taccaatacg attgctctgc
     5041 caaggctgcg gtggctcagt ttttgggctt gctggcagaa ggtaaattgg acgcggtcac
     5101 gcttgggaac ttggaaaagg ccaatgaccg cagccggtcg gcgaaggttg gcgaacgtac
     5161 tttagacggc tggatttctg cttatttgaa agcggaaaac gcgacggagc ggttggttgc
     5221 tttggctccg aagacgacga aggcggtcaa gccgattgaa agttacggat ggttgccgat
     5281 gtttatgcag tttcacaata ttccgtccgc gccaaagctg gcgcacagct accgccggtt
     5341 tgtgcagtgg gcggaagcgg aaaatatgcc ggtcaatgat gtgcctaact tgagtatggt
     5401 gcggcgcgtt tgggacaagc tgccgctgat tatgcaggag cgcggcagga aaacgggggc
     5461 ggcttataaa tcgctgctgc cttatgtgaa acgtgattgg ggggctttga agccgaacga
     5521 tgtttggatc ggcgacggcc acagctttaa ggcgaaggtg gctcatccgg tacacggcag
     5581 gccgtttaag ccggaagtga cggtgattat tgatggttgt acgcggtttg tggtgggttt
     5641 ttcggtgtcg ttggctgaaa gttgtgtggc ggtatcggac gctctgcgta tcggggtcaa
     5701 gcactttggt ttgccgatta tctactactc ggataacggt ggcggccaaa ccggcaagac
     5761 gatagaccat gaaatcacgg gtattacgtc ccgattgggt atccggcatg aaacgggtat
     5821 cgcgggcaac ccgcaaggcc gaggcatcat cgagcgatgg tggaaagaca atctgattga
     5881 gatggcgcgc cagtatgaga cgtttgcggg cgcggggatg gacagcagta ccaagaacct
     5941 gatgtaccgc aagatggaaa gtgcgtttaa cgctttggaa aaaggcaagg atttgacgga
     6001 ggaacaacag aaatatttga aaaaactgcc gggctggtcg cgttttatcg cggatgtggt
     6061 caagtgtatc gacgaataca acaaccgccc gcacggcgag ctgccccgac atccggacgg
     6121 cgggcattat tcgccgaagg cttatcggga aatgaggctg gaacaggacg gtatcgcgcc
     6181 ggatatgttg tcggcgcaag agctggcgac gatgtttatg ccgcaagagg tgcgaaaggt
     6241 acagcgcggt tggctggatt tgttcaacaa ctcttatttt tcggtggagc tggcggagta
     6301 tcacaaagac gaggtacggg tcagctacga tttgagcgat gcgtcggcgg tcaatgtgtt
     6361 tgatatggac ggcaagttta tcactaaggc gcaggccaac ggcaataccc gcgaggcttt
     6421 cccgacggct cgtatcgacc aactggcgga aaaacgccga aaaggcaaaa tcaagcgggc
     6481 ggaaaatgca atcaagctcg caaatgcaga agtcaatcct gctttggaac aggctgcggt
     6541 ttgggacgag ctgggacatt tgggcggaaa cgtcatcgag gcggagtatg cggtattgcc
     6601 gaaaacgggc acggacgacg ggattttctt gtttgaggcg gatagaagtt aaaacggttt
     6661 taaacctctt ttaaaaggac taaaaaaatg aaacaaatca atcaagcatt gcaacaaaaa
     6721 ctggctgaat ttaaagaaaa atcgggtatg agccaaaccc aactggcacg cggtatcggt
     6781 acttcgccgg catccgtcag tatgtatctg aacggtactt atgcggaaaa aggcggcaat
     6841 tatgaaacca tcgagccaaa aatcgaggcg tttttggaga tgcaggacag caaagcgaaa
     6901 cgcgaagagc tggtgttggg ttttgtatcg actaagacga cacgccgaat cgtggaagcg
     6961 atgcgcaatg cgcacgaagg cggcgagatt tccgttatct acggtcaggc gggtttgggc
     7021 aagacgcagg cggtcaaaaa ctactgcgag aaaaaccctg cagccatctt gattgaggct
     7081 aatccgagct ttacggcttt ggtcttgatg cgcaagttgg cgacggcggc gaaggtatcg
     7141 gcgatgggca gcttgaatga tttgtttgaa tcggtatcgg acagattgcg tgattcaggc
     7201 catctgattg tggtcgatga agcggaaaac ctgccattac gcgcccttga gattatccgc
     7261 cgactgcacg atgagactgg ctgcggtttg gtgttgagcg gtatgccccg actggtggcg
     7321 aatttgcgcg gtaagtatgg cgagttggta cagcttcaca gtcgtgtggc gggtgcgctg
     7381 aatctaggtg atacgatgcc cgacgcagag ttggaacaga ttgcgcgggc aaccctaccc
     7441 gaagccgatg atgaaacgat tgcggaactg gttaagcata gcaatggtaa tacacggcgt
     7501 atgagcaagt tgatgcgcgg atcagtacgg gtagcgaata aaaacggtat tcgaatgcaa
     7561 gcgggaatcg tgaaaaaata ttcgacattg attatccgat aggccgtctg aaacggtaag
     7621 tctctgacag ggctatatat tttttaccct atgattttaa taagttattg ttttctaaag
     7681 gaaaactcaa aatgcaagtt ttgaagaaag ttgattggaa gatgtttgtg gcacgctctt
     7741 tttggcagtg tgtgccggtt ggtttgacgg cgggcgtgtg gtgttttgtg gcgggaatgg
     7801 cgttgcattc ctgcgcgaaa gaacccgaac cggttgcgaa agagccgacg aaggttgagg
     7861 cgatggaaag acaggcggat ttggaggtca aggtatggga agggcaatac gagtcaatga
     7921 gcgtcgagga aaaaatgaaa gggattgttt atgagtgata agccaatttt attgagccct
     7981 gcggcgaaaa aagaggcttt ggcacgggcg gtaaaggaaa tccgcgcgaa atatggcgat
     8041 aaggcgattg tgaaaggatg tgtgaaatga gtttcggacg acgtaatacg gattggcagg
     8101 cttggggaca acaccgcagg cgtgcgacgg cgcgaatggc gcaaaaaagc cgggagcgcg
     8161 aaatcgagga atatcaggcg cagtttgaag ataaggacgg caaaggtcgt ctgaaaacgg
     8221 aaggaaacaa agatgaatga aaaagattta atcgaatggc tcgaagaccg tggcgagctg
     8281 atggtcatga agaaggacgg cgaaggcttt gtgattgcag cgcgtgcgcc ggacgggatg
     8341 tggaagactg ccgaggcgga aactttggct caggcgataa ctttatggga ggaagtgtga
     8401 tgtttaagtt tttgagaaac cgcgcgaaag ttgaagcaat tttaaacaag aaaggaaagt
     8461 gaaatgaaag tgtatgtttt taaaattagt aatgaaaacg gtaagttaaa gatagaactc
     8521 cccgaaatcc caatgggtaa gcaaattgac gaagttgatt tgattgctgg gctaaccacg
     8581 gaatttattg caagtatgtt acgtgatgcc caaaaagatc gtcgcaaatt cgtaatcgac
     8641 gcatcaaatc aattggctgc aatccagaca tatcaaaaaa tcttcaatta aggaaggaag
     8701 agaaaatggc taaaacccga atcaaacagc ccgccatcga agcggcacaa gacaaaacgg
     8761 aagttactgc atttatccgc caaatcggcg acttgcagcg cgaagtcaaa cgcctggaaa
     8821 ccgaagccgg agacaaaaaa gcggctatcg aagaagaata tgccgccaaa gccgcgccga
     8881 tgtgtgccga aatcatgagc ctgaccgaac gtgtggccgc ctactgcgaa gcgcataagg
     8941 acgagctgac ggaaaatggt aaaaccaaaa ccgtggactt taccaccggc ctgattaaat
     9001 ggcgcatccg tccgccatct gtcaaggtaa cgggcgtggc cgccgtcctg gcgtggctct
     9061 cggaaaaatc cgcctttgcc gagtttgtcc gcactaaaaa ggaaatcgac aaagacacca
     9121 tcctgaatca aaaagagtgt ttttcagacg gccaagttcc ggggattaag attgtgagcg
     9181 gcgtggagga ttttgtgatt gagccgagcg aacaaattct ataaaatcaa cagcctgatt
     9241 tcaattcagt gtgttgaaac gtgattgagc ctacggagca ggagcttgcc tagccggttc
     9301 aaataggctt taacccatga tttaaagg
//
LOCUS       DAWFAD010000073         9325 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWFAD010000073 DAWFAD010000000
VERSION     DAWFAD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.073"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            168..1511
                     /locus_tag="WGO49_001286"
     CDS             168..1511
                     /locus_tag="WGO49_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ0306394.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     DRYAPDALAKLSGKEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTGRVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LLGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            1514..2746
                     /locus_tag="WGO49_001287"
     CDS             1514..2746
                     /locus_tag="WGO49_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ0306395.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFAVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            2739..3515
                     /locus_tag="WGO49_001288"
     CDS             2739..3515
                     /locus_tag="WGO49_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ0306396.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            3515..4141
                     /locus_tag="WGO49_001289"
     CDS             3515..4141
                     /locus_tag="WGO49_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ0306397.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            4145..4738
                     /gene="nqrE"
                     /locus_tag="WGO49_001290"
     CDS             4145..4738
                     /gene="nqrE"
                     /locus_tag="WGO49_001290"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ0306398.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            4752..5969
                     /gene="nqrF"
                     /locus_tag="WGO49_001291"
     CDS             4752..5969
                     /gene="nqrF"
                     /locus_tag="WGO49_001291"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ0306399.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPEEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            6121..7176
                     /locus_tag="WGO49_001292"
     CDS             6121..7176
                     /locus_tag="WGO49_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0306400.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKSGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     TGGYRTAMSSEFEKLLR"
     gene            7202..7417
                     /gene="nqrM"
                     /locus_tag="WGO49_001293"
     CDS             7202..7417
                     /gene="nqrM"
                     /locus_tag="WGO49_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ0306401.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRTIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(7541..8119)
                     /gene="grpE"
                     /locus_tag="WGO49_001294"
     CDS             complement(7541..8119)
                     /gene="grpE"
                     /locus_tag="WGO49_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ0306402.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            8303..9121
                     /gene="cysE"
                     /locus_tag="WGO49_001295"
     CDS             8303..9121
                     /gene="cysE"
                     /locus_tag="WGO49_001295"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ0306403.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
BASE COUNT         2252 a         2624 c         2314 g         2135 t
ORIGIN      
        1 taaaaacggc ttccgcaagg aaaaaagcag caaatctgac aaacaggtac aattcctgat
       61 agaatctaac actttgctac attaatttat tcaatgcttt tgatgccacg cgacacggta
      121 tcaaaacacg ccgtttttac cttaaccatc acaagtaatg caaatatatg attaaaatca
      181 aaaaaggtct aaacctgccc atcgcgggca gaccggagca agtcatttat gacggcccgg
      241 ccattaccga agtcgcgttg cttggcgaag aatatgccgg tatgcgcccc tcgatgaaag
      301 tcaaggaagg cgatgccgtc aaaaaaggcc aagtgctgtt tgaagacaaa aagaatccgg
      361 gcgtggtgtt taccgcgccg gcttcaggca aaatcgccgc catccatcgc ggcgaaaagc
      421 gcgtacttca gtcggtcgtg attgccgttg aaggcaacga cgaaatcgaa ttcgaccgct
      481 acgcgcccga tgccttggca aaattgagcg gcaaagaagt gcgccgcaat ctgattcaat
      541 ccggtttgtg gactgcgctg cgtacccgtc cgttcagcaa aatccctgcc gtcgatgccg
      601 agccgttcgc catcttcgtc aatgcgatgg acaccaatcc gctggcggca gaccctgtgg
      661 ttgtgatcaa agaagccgcc gaggatttca gacgaggttt gctggtattg agccgtttga
      721 ccgagcgtaa aatccatgtg tgtaaagcag caggcgcaga cgtgccgtct gaaaacgccg
      781 ccaacatcga aacacatgaa ttcggcggcc cgcatccggc cggtttgagt ggcacgcaca
      841 ttcatttcat tgagccggtc ggtgcaaaca aaaccgtttg gaccatcaat tatcaagatg
      901 taattgccat cggacgtttg tttgcaacag gccgtctgaa caccgggcgc gtggttgcct
      961 tgggtggttc tcaagtcaac aaaccacgcc tcttgcgtac cgttttgggt gcgaaagtat
     1021 cgcaaattac tgcgggcgaa ttggttgacg cagacaaccg cgtgatttcc ggttcggtat
     1081 tgaacggcgc gattgcacaa ggcgcgcacg attatttggg acgctaccac aatcagattt
     1141 ccgttatcga agaaggccgc agcaaagagc tgctcggctg ggttgcgccg cagccggaca
     1201 aatactccat cacgcgtaca accctcggcc atttcctgaa aaacaaactc ttcaagttca
     1261 acacagccgt caacggcggc gaccgcgcca tggtgccgat tggtacttac gagcgcgtga
     1321 tgcccttgga tatcctgccc accctgcttt tgcgcgattt aatcgtcggc gataccgaca
     1381 gcgcgcaggc attgggttgc ttggaattgg acgaagaaga cctcgctttg tgcagcttcg
     1441 tctgcccggg caaatacgaa tacggcccgc tgttgcgcaa agtgctggaa accattgaga
     1501 aggaaggctg attatgggct tgaaacattt tctggaaaaa atcgaaccgc acttcctgcc
     1561 gggcggcaaa catgaaaaat ggtatgccct ctacgaagcg gcggcgacga ttttctacac
     1621 atccggtgcg gtaacgcgca aagcggcaca cgtccgcgac gcgctcgact ccaagcgcat
     1681 gatgattttg gtgtggctgg ctttgttccc cgccatgttt tacggcatgt acaacgtcgg
     1741 cgcacaggca ttcggtgcct taacgcccga tttgctgcaa caaagcatcg ccaacgactg
     1801 gcattacacc cttgccgacg ctttgggcat caatatgtcg tctgaagcgg gcgtgttggg
     1861 caaaatgctg ttcggcgcga tttacttcct gccgatttac gcgaccgtat ttgccgtcgg
     1921 cggtttctgg gaagtcttgt tcgccaccgt acgcaaacac gaaatcaacg aaggtttctt
     1981 tgttacctcg attctgtttg ccttaatcgt tccgcccacg ctgccgctgt ggcaggcggc
     2041 tttgggtatt tctttcggcg ttgtggttgc gaaagaagta ttcggcggta cgggtaaaaa
     2101 ctttatgaac cctgcgctgg caggccgcgc cttcctgttc ttcgcctacc ccgccaactt
     2161 gagcggcgat gcggtttgga cggcggttga cggctattcc ggcgcaaccg cgctggcgca
     2221 atgggcggca cacggtgcag acggcctgaa aaacgccgta accggtcaaa ccatcacttg
     2281 gatggacgcg tttatcggca aactgcccgg ctccatcggc gaagtctcca ctttggcact
     2341 cttaatcggc ggcgcgttta tcgtgtttgc ccgcatcgct tcttggcgca ttattgccgg
     2401 cgtgatgatc ggtatgattg cgatgtcttc gctgttcaac ttcatcggtt cggacaccaa
     2461 cgctatgttt gccatgcctt ggtactggca tttggtcgtc ggcggcttcg ccatcggtat
     2521 gctgtttatg gcgaccgacc ctgtttccgc ttcctttacc aatgtcggca aatggtggta
     2581 cggcgcgctg atcggcgtga tgtgcgtgtt gatccgcgtg gtcaatccgg cttaccccga
     2641 aggtatgatg ttggcgattc tgtttgccaa cctgtttgcc ccgattttcg actatttcgt
     2701 cgcacaagcg aacatcaaac gcagaaaggc gcgcagcaat ggctaagaaa ttcgataaag
     2761 acagcttcag cggcacgctg attgtcgtgt tggcggtcag cctgatttgc tcggtcatcg
     2821 ttgccggtgc ggtcgtcggc ttgaaaccca tccaagagaa acaaaaactc caagacaaac
     2881 aaggctatat cttgagcgta gccggtttga tggataagga caccgacatc ggcaaaacct
     2941 ttgccgagcg tatcgagcaa cgtgttgtcg atttggcgac cggcgaatac gtcgccgatg
     3001 cgcctaaaga cttcagcgcg cgcatcgcag gcaaagaccc tgcccaaagc atccgcatca
     3061 aacccgaaga cgatttggca ggcatcaaaa gccgtgccaa atacaccgag gtttatttgg
     3121 taaaaggcga agacggcaaa atcgggcaaa tcatcctgcc tatgcacggt aacggtttgt
     3181 ggtcggtcat gtacggcttt gtcgccatcc agcccgacgg caacaccatc aacggcatta
     3241 cctactacga acaaggcgaa accccgggct tgggcggcga aatcggcaat ccgttgtggc
     3301 agcaaaaatt cgtcggcaaa aaactgtttg acggacaagg caaactcgcc ctgcacgtcg
     3361 gcaaaggcgc gggttcggac aaagaacacg gcgtagatgc cctctccggc gcatcgctga
     3421 catccaaagg cgtgcaaggt tcgttcgcct actggttcgg cgaaaacggc tatatcccct
     3481 acctgaacaa attgaaatca gcaggagcgc aataatggct gatatgaaac gcttgaaaca
     3541 tttgatgttt tcacccttta tcgacaacaa cccgattgcc ttgcaggttt tgggtatttg
     3601 ttcggctttg gcggttacca ccaaacttca gacggccatc gtgatgggta tttccgtagc
     3661 tttggtaacc ggtttttcca gcttcttcat ttcgctggta cgcaactaca tccccaacag
     3721 catccgtatt atcgtgcaaa tggcgattat cgcgtcgctg gttacgctgg tcgaccaact
     3781 cttgcaggca tttgcctacg aattgtccaa acagctttcc gtattcgtcg gtctgattat
     3841 taccaactgt atcgtgatgg gccgcgccga agcatttgcg atgaaagagc cgccgctgga
     3901 aagcctgatc gacggcatcg gcaacggcgc gggctacggg ataatgctgc ttgtcgtcgc
     3961 caccgtccgc gaactgattg gctcgggcaa actcttgggc tacaccgttt tccaaaccgt
     4021 gcaggacggc ggctggtatc agaccaacgg cttgttcctg ctcgccccca gcgcgttctt
     4081 catcatcggc tttttgattt ggggactgcg tacctggaaa cccgaacagg cggaggaata
     4141 agctatggaa cactatttga gcctcttcat caaatccgtc ttcattgaaa atatggcact
     4201 atccttcttt ttgggtatgt gcacgttttt ggcggtatcc aaaaaagtat ccaccgcatt
     4261 cggtttgggt gtggcggtaa ttttcgtact cgggctgtcc gtccctgtca accaactcgt
     4321 ttactcgctg ctcaaagacg gcgcgattgt cgaaggcgtg gatttgacct ttttgaaatt
     4381 catcaccttc atcggcgtga ttgccgcttt ggtgcagatt ttggaaatgt tcttggataa
     4441 attcgtcccc gccctctata acgcattggg catctacctg ccgctgatta ccgtgaactg
     4501 cgcgattttc ggcgccgtat cgtttatggc gcaacgcgaa tacaacttcg gcgagtccgt
     4561 tgtatacggc ttcggcgcgg gcttgggctg gatgttggcg attgtcgctt tggcgggcat
     4621 taccgaaaaa atgaaatatt cggacgctcc caaaggcctc aaaggcttgg gcattacctt
     4681 tatcgccgcc ggcctgatgg cgatggcgtt tatgtcgttc tctggcatcc agttataaga
     4741 aagggatcgg tatggagatt attttaggta tcgtgatgtt taccgtcatc gttttggttt
     4801 tggcactgat gattctgttt gccaaatcca aactggtcag cgaaggcgac atcaccatca
     4861 aagtcaacgg cgaaaaagag ctgacgatgc ccgccggcgg caaactcttg ggcgcgcttg
     4921 ccaacgaagg catctttatc ccctccgcct gcggcggggg cggttcgtgc ggacaatgcc
     4981 gcgtcgtcgt aaaaagcggc ggcggcgaca tcctgccgac cgagctgtcc cacatcagca
     5041 aacgcgaagc acgcgaaggc tgccgtctgt cgtgccaagt caacgtcaaa accgacatgg
     5101 acatcgaagt ccccgaagaa gtgttcggcg tgaaaaaatg ggaatgcacc gtcatctcca
     5161 acgataacaa agccacgttc attaaagaac tcaagcttgc cattcccgaa ggcgaagaag
     5221 tccccttccg cgccggcggc tacattcaaa ttgaagcccc gccgcacacc gttgcctaca
     5281 aagacttcga cattcccgag gaataccacg aagactggga caaatacaat ttgtggcaat
     5341 acgtttccaa agtggacgag ccgattttgc gtgcctactc tatggcttcg tatcctgaag
     5401 aaaaaggcat cattatgctg aacgtgcgta tcgccacgcc gcccccgcgc gtacctgatg
     5461 cgcctccggg acaaatgtcg tcctacatct ggtcgctcaa acccggcgac aaagttacga
     5521 tttccggccc gttcggcgaa tttttcgcca aagacaccga tgccgaaatg gtatttatcg
     5581 gcggcggtgc gggtatggct ccgatgcgtt cccacatttt cgaccagttg aaacgtttga
     5641 actccaaacg taagattacc ttctggtacg gcgcacgttc caaacgcgaa atgttctatg
     5701 tcgaagactt cgaccaactc gcagcagaat tcccgaactt cacatggcac gtcgccctgt
     5761 ccgacccatt gcctgaagac aactgggacg gctacacagg cttcattcac aacgtggttt
     5821 acgaaaacca tctgaaaaac cacgaagcgc cggaagactg cgaattttat atgtgtggtc
     5881 ctccgatcat gaaccagtcc gtcatcaaaa tgcttaaaga cttaggcgtg gaagacgaaa
     5941 acatcctctt ggacgatttc ggcggttaaa tattcggctt caaacacaaa aggaagaaac
     6001 ctcggtttct tcctttttct atccgatgcc gcccgaaccc ccttcagacg gcatcggcat
     6061 agaaacatat cggccgtttt gcgatataat tcaaaccatt tgccaaaacc gtcaaacact
     6121 atgccgtctg aaacacgcct gccgaacttt atccgcgtct tgatatttgc cctgggtttc
     6181 atcttcctga acgcctgttc ggaacaaacc gcgcaaaccg ttaccctgca aggcgaaacg
     6241 atgggcacga cctataccgt caaatacctt tcaaataatc gggacaaact cccctcacct
     6301 gccgaaatac aaaagcgcat cgatgacgcg cttaaagaag tcaaccggca gatgtccacc
     6361 tatcagcccg actccgaaat cagccggttc aaccaacaca cagccggcaa gcccctccgc
     6421 atttcaagcg acttcgcaca cgttactgcc gaagccgtcc gcctgaaccg cctgacacac
     6481 ggcgcgctgg acgtaaccgt cggccccttg gtcaaccttt ggggattcgg ccccgacaaa
     6541 tccgttaccc gtgaaccgtc gccggaacaa atcaaacaag cagcatctta tacgggcata
     6601 gacaaaatca ttttgaaaca aggcaaagat tacgcttcct tgagcaaaac ccaccccaag
     6661 gcctatttgg atttatcttc gattgccaaa ggcttcggcg ttgataaagt tgcgggcgaa
     6721 ctggaaaaat acggcattca aaattatctg gtcgaaatcg gcggcgagtt gcacggtaaa
     6781 ggcaaaaacg cgcgcggcga accttggcgc atcggcatcg aacagcccaa catcgtccaa
     6841 ggcggcaata cgcagattat cgtcccgctg aacaaccgtt cgcttgccac ttccggcgat
     6901 taccgtattt tccacgtcga taaaagcggc aaacgcctct cccatatcat taatccgaac
     6961 aacaaacgac ccatcagcca caacctcgcc tccatcagcg tggtcgcaga cagtgcgatg
     7021 acggcggacg gcttgtccac aggattattc gtattgggcg aaaccgaagc cttaaagctg
     7081 gcagagcgcg aaaaactcgc tgttttcctg attgtcaggg atacaggcgg ctaccgcacc
     7141 gccatgtctt ccgaatttga aaaactgctc cgctaaacat acaaaccgat taggaaacac
     7201 catgaaaacc ctgctcctca ccttcggcat cttcctgacc gtcatcatcg gtatggcggt
     7261 cggctatatc ttctccaaac gcaccatcaa aggcagttgc ggcggcatta ccgccttggg
     7321 tatgaaaaaa atgtgcgact gcgacacgcc ttgcgacacc ctgcaaaaga aactggatga
     7381 agaaaaacag gcaggcggca tacgggttga ccgttaacac cgaaacaaaa tgccgtctga
     7441 agcccgacag gcgggttcag acggcattgc cgctttactt cgatcggttt atacgcaaaa
     7501 cgcgcttcag gaaataaatc agattattcc ccagacgcct tcaggcttcc ttctgtgcca
     7561 ccgtaaccat agccgggcgc aacacgcggt cggacagcgt ataacccttc ttcatcacgc
     7621 ccaccacggt attgggttcc tgttcgcttg ccaccgcctg catcgcctga tggatattcg
     7681 gatcgagctt atcgcccgct ttaggattga tttccttgat ttgcgtagca tcaaatgctt
     7741 tctgcaactc gttcaaagtc atctgcacgc ccattttcag cgcatcgaaa ttgccgctct
     7801 gatccaaaag cgccatttcc agataatcct tgaccggcag catttccacg gcaaacttct
     7861 gtccggcgaa cttgtgcgta tccgcaattt cctgctggtg gcggcggcgc aggttttgct
     7921 cgtttgccaa agcgcgcagc tgctcgtctt tcaactgcgc ttccagctcg gcaatccgcg
     7981 cctgcaaatc ctcataagcc ggctctgcgg cagcctgttc ctgcacaccg tccgcatttc
     8041 ctactgtctc gacggtttcc accgcctcca cattttcaac cgcttcttca ctgttttgct
     8101 gctgtgtctg ttcgctcata tcgtatccct taaaacaaat tggaaatcaa aatccagtta
     8161 ttacccgcgc aagataagga cattttcaag aaacttcaag caaaagcaga gaatttcaaa
     8221 ttcattttca aatcccctac cgaaaaaata atatagacgg taagatactg ccgaccatat
     8281 cgattttcaa acgtcatcac tcatgaaaaa agaccattta aacacaaccg gtttcgacct
     8341 ctggcacacc atccgcgaag aaaccgcggc cgcggtctcc gccgaaccga tgctggcaag
     8401 ttttttgcac caaaccgtgt tgcgccacga gtccctcggc tccgtccttg cctaccacct
     8461 ttccagcaaa ctcggcagcc cgattatgga cgtgcgcgcg ctgtttgaaa tctaccagca
     8521 ggcgttgggc agcgacaccc aaatcggcaa atgcgtcgaa gcagacttaa aagccatcta
     8581 cgaacgcgat cccgcctgcg acgaatattc gctgccgctt ttatatttca aaggcttcca
     8641 cgcgattcag gcacaccgca tcaaccatcg gctgtatctc gacgggcgca aaacgctggc
     8701 gtatttcctg caaaaccgta tgtccgaagt attcggcgtg gacatccatc ccgccgcccg
     8761 tttcggatac gggctgatgc tcgaccacgc caccggcttt gttgccggcg aaaccgccgt
     8821 gttgggcaac aatatttcga ttttgcacgg cgtaacgctc ggcggttcgg gcaaagaagg
     8881 cggcgaccgc caccctaaaa tcggcgacgg cgtgatgatc ggcgcaaacg cctcgatatt
     8941 gggcaatatc cgcatcggca gcaatgccaa aatcggggcg ggcagcgtcg tggtttcaga
     9001 cgtgccgccg tccatcacgg ttgtcggcgt acccgccaaa cccgtggcgc gatcgctcaa
     9061 aacaccgtcg gcggatatgg atcaaaatat ccagtttacc gaaatcgact ttatgatttg
     9121 aatatccgca ccaatgccgt ctgaaacgcc aaacgggctt cagacgacat tgcccgcttc
     9181 aagcctgcgc cttgatatgc cgccgatgtc ggcacttgcc agtcggacaa aatgcctttt
     9241 attcaataca cccgattccc acgcaaccgg aaatatagtg gattaacaaa aatcaggaca
     9301 aggcgacgaa gccgcagaca gtacg
//
LOCUS       DAWFAD010000074         9290 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWFAD010000074 DAWFAD010000000
VERSION     DAWFAD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.074"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..402)
                     /locus_tag="WGO49_001296"
     CDS             complement(<1..402)
                     /locus_tag="WGO49_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0306404.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTRE"
     gene            641..1540
                     /gene="crgA"
                     /locus_tag="WGO49_001297"
     CDS             641..1540
                     /gene="crgA"
                     /locus_tag="WGO49_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ0306405.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1767..4982)
                     /gene="carB"
                     /locus_tag="WGO49_001298"
     CDS             complement(1767..4982)
                     /gene="carB"
                     /locus_tag="WGO49_001298"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ0306406.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFKLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQVLGYGICATRGTA
                     QYLTEQGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4989..5537)
                     /locus_tag="WGO49_001299"
     CDS             complement(4989..5537)
                     /locus_tag="WGO49_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306407.1"
                     /translation="MRTIFFSGSRSISRLNPQIRERINNILSNNFDIVIGDANGADKA
                     IQKFLQEQDYANVHIYFSGKIYRNNVGNWQFVQVDSKGTGRVFYTAKDKKMAEIADYG
                     FILWDGKSIGSLNNIAELLQLNKPSLVYHSQTKEFFKIKSSADLENILSNIEDDVLAS
                     ILEKGNTFLKSYVTKQPSLIQE"
     gene            complement(5540..6103)
                     /locus_tag="WGO49_001300"
     CDS             complement(5540..6103)
                     /locus_tag="WGO49_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0306408.1"
                     /translation="MAIELNGNWTKGFALDHHMQKSILLGYDELGHPHFDSKRSVIGE
                     WVYQLKYRNQTQNVSLLVDYILQKFSGLESINLIVPAPFTTERINQPVQLIAKELSDR
                     LNIPYSPILRKSSSHTPLKNIEEKSEKLEILNNSITIDNIDLSNKNILVIDDLFDSGA
                     TLEISTEKLFSKNARSVIVLAMTKTRG"
     gene            complement(6510..6677)
                     /locus_tag="WGO49_001301"
     CDS             complement(6510..6677)
                     /locus_tag="WGO49_001301"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306409.1"
                     /translation="MADFDWIIENNRIKPTELSIYKGRLKNKKQPAQPVFPAEPLIPT
                     AAPSSLPVGKR"
     gene            complement(6681..7070)
                     /locus_tag="WGO49_001302"
     CDS             complement(6681..7070)
                     /locus_tag="WGO49_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ0306410.1"
                     /translation="MSTHEKLLTADNPVLHQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7662..7787)
                     /locus_tag="WGO49_001303"
                     /pseudo
     CDS             complement(7662..7787)
                     /locus_tag="WGO49_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7833..8966)
                     /gene="carA"
                     /locus_tag="WGO49_001304"
     CDS             complement(7833..8966)
                     /gene="carA"
                     /locus_tag="WGO49_001304"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ0306411.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWELGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         2111 a         2335 c         2377 g         2467 t
ORIGIN      
        1 ttcgcgggta aacgcctcaa tcggcgcatt ccaagtcagt gaaagcatat gttttttgcc
       61 ttgcaacaag ccgcctgtgc cgtagccctc agtcggattg acgcggtgtc tgccgtcgct
      121 ttggtagagt ttgccgtgtc cagcggttaa tacttcgtct atgtattttt tcactgtcca
      181 aggctcgtgc atccaccagc ccggcatctg ccaaatcacg gcatccatcc aaacgaattt
      241 ttcgatttct gcctcaacat catagccggc atcaatcacg gtttcttgaa cattgtgtcc
      301 gagcgcggtc aaaacttctt tcgctttttt gtgaagcgtg cggtttaacc cgccgtgaga
      361 atgtccgaac gccttgccgc cgtctaataa taaaatattc atttgttacc tcgtttgtga
      421 attgatgaat gtattgtgcc gttttacttt ataatttaaa agtgcaaaaa taagaaaaca
      481 cttttgcgtc aaatgaaata atcagatgtt tcaacacacg ggatgacaca taaagcgtct
      541 ccctatgtgc cgtcctgatt cggaaggggt tacacccctc ccaaataaag tctgatcctg
      601 ccgccctaaa gggcggggtt ccaaccgaaa aggaaacacg atgaaaacca attcagaaga
      661 actgaccgta tttgttcaag tggtggaaag cggcagcttc agccgtgcgg cggagcagtt
      721 ggcgatggca aattctgccg taagccgcat cgtcaaacgg ctggaggaaa agttgggtgt
      781 gaacctgctc aaccgcacca cgcggcaact cagtctgacg gaagaaggcg cgcaatattt
      841 ccgccgcgcg cagagaatcc tgcaagaaat ggcagcggcg gaaaccgaaa tgctggcagt
      901 gcacgaaata ccgcaaggcg tgttgcgcgt ggattccgcg atgccgatgg tgctgcatct
      961 gctggcgccg ctggcaacaa aattcaacga acgctatccg catatccgac tttcgctcgt
     1021 ttcttccgaa ggctatatca atctgattga acgcaaagtc gatattgcct tacgggccgg
     1081 agaattggac gattccgggc tgcgtgcacg ccatctgttt gacagccgct tccgcgtaat
     1141 cgccagtcct gaatacctgg caaaacacgg cacgccgcaa tctacagaag agcttgccgg
     1201 tcaccaatgt ttaggcttca ccgaacccgg ttctctaaat acatgggcgg ttttagatgc
     1261 gcagggaaat ccctataaga tttcaccgca ctttaccgcc agcagcggtg aaatcttacg
     1321 ctcgttgtgc ctttcaggtt gcggtattgc ttgcttatca gattttttgg ttgacaacga
     1381 catcgctgaa ggaaagttaa ttcccctgct cgccgaacaa acctccgata aaacacaccc
     1441 ctttaatgct gtttattaca gcgataaagc cgtcaacctc cgcttgcgcg tatttttgga
     1501 ttttttagtg gaggaactgg gaaacaatct ctgtggataa ttctataaaa acctgccata
     1561 aaattgtttt tactcactta ataactttga gaaaaaataa ataagatgat gtatagccgg
     1621 cggcaatggc agtctatatg gggagcgtga tagggctgtt ccttatgtac ggcttgtgtg
     1681 tggcaacatc ggctttccaa atgacaaaat gccgtctgac accgtaaaaa aaggtttcag
     1741 acggcatttt caacctgatt caggcatcag ttgcggtttt tcaaacgccc gtgcaactct
     1801 tgaacgctgt acacgcccag atggtctcgg cttttcgcac cttcgctcat cgcttcgccg
     1861 ccggcagtgg tggtgtattg cggcacacgt tgctgcaatg cgctttggcg gatgatgtgg
     1921 ctgtcggaca cggattgcgg atcgctggaa acggtattca cgaccagtgc gatttcaccg
     1981 tttttcagtg cgtcgccgat gtgcgggcgg ccttcgggga ctttattgat ggtttgtaca
     2041 atcagtccct gttcggtcag gtattgcgcc gtgccgcgtg tggcgcagat gccgtagcct
     2101 aaaacctgga agtttttagc ggttttgata acgcgttctt tgtcttcttc gcgcacggag
     2161 aggaagattt tgccggtcgg gttgaggcgt tcgcccgcac cgagttgggc tttgtagtag
     2221 gcttcgccga agcttgcgcc cacgcccatc acttcgccgg tggagcgcat ttccggtccc
     2281 aaaatcgtgt ctacgcccgg gaatttgatg aatgggaaca cggcttcttt aacggcatag
     2341 aaatcgggga caacttcttt ttccacgcct tgttctttca gggaaatacc tgccatgcag
     2401 cgtgcgccga ctttggcgag cggcacgccg gtggctttgg agacgaaggg cacggtacgg
     2461 ctggcgcgcg ggttcacttc caacacgaac accacgccgt cctgcacggc aaactgcacg
     2521 ttcatcagac cgaccacgcc cagcgcgtac gccatcgctt tggtttggcg gcggatttcg
     2581 tcttggattt cttcgcttaa ggagtagggc ggcagcgagc agccggagtc gccggagtgg
     2641 atgcccgcct gttcgacgtg ctgcataatg ccgccgataa ccacgtcttt gccgtctgaa
     2701 acgcagtcca catcgacttc aatcgcgttg ttgaggaaga aatcgagcaa cacggggctg
     2761 tcttcggaaa cctgcacggc ttcgcgcatg tatttttgca aggcttcggc agagtggaca
     2821 atctgcatgg cgcggccgcc caaaacataa gacgggcgca cgaccagcgg atagccgatt
     2881 tcttcggctt tgacgagtgc ttcttcttcg ttgtgggcga tgcggttggg cggttggcgc
     2941 agacctaagt cgttcaacac tttttggaag cgttcgcggt cttcggcggc gtcgatgctg
     3001 tcggcggatg tgccgatgat gtttacgccg ttttcaacca gcgcgttggc gagtttgagc
     3061 ggggtttgac cgccgtaatg cacaatcacg ccccacgggt tttcggtgcg gacgatttcc
     3121 aacacgtctt ccaatgtcag cggctcgaaa tagaggcggt cgctggtgtc gaagtcggtg
     3181 gacacggttt ctgggttgca gttcaccata atcgtttcaa agcccgattc gcgcagggcg
     3241 agtgcggcgt gaacgcagca gtagtcaaac tcgatgccct gaccgatgcg gttcgggccg
     3301 ccgccgagaa tcatcacttt tttacggtcg gaaggacggg attcgcattc ttcttcgtaa
     3361 gtggagtaaa gataggcggt ttcggtggcg aactcggcgg cgcaggtgtc aacgcgtttg
     3421 taaaccggat gcagcttcag cgcgtagcgg tgttcgcgca cttctttttc gcttacgttc
     3481 aacaattgtg ccaaacgttt gtcggagaag cctttgcgtt tcagacggcg tagggcggcg
     3541 aaatccaaat cactcaaaat gccgtctgaa accgcttttt cttccttcat caagtcttcg
     3601 atttgcgcca ggaaccaagg gtcgatggcg cagatttcgt ggatttcttc cagcgtgaag
     3661 ccggcgcgga acgcgtctgc cacaaacagc atacgttcgg ggccggggtt tgccagttcg
     3721 cggcggattt ccgctttgtc ggagcttctc ggattgaagc cgcacaagcc tgtttccaag
     3781 ccgcgcaggg ctttttggaa gctttcctga atcgtgcggc ccatcgccat cacttcgccc
     3841 acggatttca tctgcgtggt caggcggtcg tctgcggcag ggaatttttc aaacgcaaaa
     3901 cgcgggattt tggttaccac atagtcgatg gacggctcga atgacgcggg ggttttgccg
     3961 ccggtgatgt cgttgcgcaa ctcgtccagc ttaaagccga ccgccagttt cgccgccacc
     4021 ttcgcaatcg ggaagcccgt cgctttggaa gccagcgcgg acgaacggct cacgcgcggg
     4081 ttcatctcaa tcacaatcat ctcgccgttt tcagggttca ccgcaaactg cacgttcgag
     4141 ccgccggtat ccacgccgat ttcgcgcaat actgccaacg aagcattacg catgatttgg
     4201 tattctttgt ccgtcagcgt ttgcgcaggc gcaaccgtaa tcgagtcgcc cgtatgcacg
     4261 cccatcgggt cgaagttttc aatcgagcag ataatgatgc agttgtcgtt cttgtcgcgc
     4321 accacttcca tctcgtactc tttccagccg agaacagact gctcaatcag cagctcgtgc
     4381 gtaggcgacg catcgaaacc gcgttcgcaa atcgccaaaa actcatcttt attgtaggca
     4441 atgccgccgc ccgaaccgcc catcgtgaaa gacggacgaa tcagggtagg gaagccgacc
     4501 tgttcttgcg ctgccaaagc ttcgttcatc gtgtggcaga caaaagattt cgggcaggag
     4561 aggccgattt tttccatcgc ttctttaaaa cgaccgcggt cttccgcctt gtcaatcgcg
     4621 tcttccgtcg cgccgatcag ctcgacattg tatttcgcca gcacgccgtt gcgcgccaaa
     4681 tccagcgcac agttcagcgc ggtctgaccg cccatcgtgg gcagaatcgc atcaggacgc
     4741 tccttggcga taatcttctc caccgtctgc cacataattg gctcgatgta ggtaacatcc
     4801 gccatttcag ggtcggtcat gatggtggcg gggttggaat tcaccagaat gactttatag
     4861 ccttcttcac gcaaagcctt acaggcctgt gcgcccgaat agtcaaattc gcaggcctga
     4921 ccgataacga tagggccggc gccgatgata aggatggatt ttaggtcggt acgtttgggc
     4981 atgggtggtt actcttgaat taaagaaggt tgttttgtta catagctttt aagaaaagta
     5041 tttccctttt ctaaaattga agctaaaaca tcatcttcta tgttagataa aatattctct
     5101 aaatctgcgc ttgatttaat tttaaagaat tcttttgttt gagaatggta tacaagagaa
     5161 ggtttattta gctgtaaaag ttctgctata ttatttaatg aaccaatact cttaccatcc
     5221 cataaaataa agccataatc agcaatttct gccattttct tatcttttgc tgtataaaat
     5281 acccttccag ttcctttgct gtctacttgg acaaactgcc aattccccac attatttcgg
     5341 taaattttgc cagaaaagta aatatgcaca ttagcataat cttgttcttg tagaaatttt
     5401 tggatagctt tatcagcccc attagcatct ccaataacaa tatcaaaatt attgctaaga
     5461 atattgttaa ttctttctcg tatttgtgga tttagtcgtg aaatgctgcg tgaaccagaa
     5521 aagaaaatag tgcgcatttt tatcctctag tctttgtcat tgctaaaaca attacagatc
     5581 tagcattttt tgaaaacaat ttttcagttg atatttccaa tgttgctccg ctatcaaata
     5641 aatcatcaat tactaaaata ttcttattgg atagatctat attgtcaatt gtgatggaat
     5701 tattcagtat ttctagtttt tctgattttt cttcaatatt tttgagtggt gtgtgtgagc
     5761 tagattttct taaaatagga gaatatggaa tattcaagcg atcacttagt tctttggcaa
     5821 ttaattgaac tggttgatta atacgctctg ttgtaaaagg ggcgggaaca attaaattta
     5881 tagattctaa accactaaat ttttgtaaaa tatagtctac cagcaaagaa acattttggg
     5941 tttgattacg atattttaat tgataaaccc attctccaat cacacttctc ttgctatcaa
     6001 agtgaggatg ccctaattca tcataaccta acaaaatact tttttgcata tgatggtcta
     6061 aagcaaatcc ttttgtccag ttaccattta gttctatcgc catttcattg actccttgtc
     6121 aaaattaagt ataacgagca atcctagcaa gccgtagccc gcatgtaggg tatgtgcggt
     6181 acgcacgcac gcgttcttca ttttccctgt aaccccaacc caccgcatgc gcgccttgcg
     6241 gcacacactc tgccgacgga ttcaaaggtc gtctgaaaaa acgctcgcgg cgtttgttac
     6301 atctgccttg cgataaaggc tgcctgaaaa attcccactg ttgatatttc gggcattctt
     6361 tctcaaaccg cctgcaaccg caaaatctgc tgaaacctac tccctccccc gtgggggagg
     6421 gctggggaga gggcattctc cgaatttcgg caacctttcc taacactcta accgccaaga
     6481 cacaagcctt gcggcttgtt gccctctctc taacgctttc ccacagggag agaggacggg
     6541 gcggctgttg gaattaaggg ttctgcagga aaaacaggtt gtgcgggctg ttttttgttt
     6601 ttcagacggc ctttataaat gctcaattca gtcggcttta tcctattatt ttcaataatc
     6661 caatcaaaat cagccatctg ctatcgtgca gcttgctttt ccaattcttg caacacgagc
     6721 aggatttctg tcagcacatc gtttgtctgc tgtaaaatct cgtgattcca aaaccgcagt
     6781 acggtaaagc ccaagctgtt gagatatgcc gtccgcgcgt ggtcgtatgc ggcttgttct
     6841 gtgtgctgcc ctccgtctgc ttcgacaatc aacttgggcg ttacgcacat aaaatcaaca
     6901 atatagtttc ccatcggctg ctggcggcgg aatttatagc cgttcagacg gcctgcccgc
     6961 aggtgctgcc acaattttgc ttccgcctcg ctcatttctt ggcgcatggc tttggcgcgt
     7021 tgatgcagga cggggttgtc ggcggttaaa agtttctcgt gcgtgctcat tctgttgtcg
     7081 ggttactgtt tgtgtaagcc ttacagtttg ttgccctctc tctgactctc tcccatggga
     7141 gagaggacgt ggtggcggtt gggattaagg gttctgcaag gaaaacaggt tgtgcaggct
     7201 gctttttatt tttcagatga cctgaaacaa cagctgcaaa tctaagttgc ctttcaaatc
     7261 gcctgcaacc gcaaaatctg ctgcaacctt ctccctcccc cgtgggggag ggctggggag
     7321 agggcattct tcgaatttcg gcaaccttcc ccaacaccct aaccgcccaa acacaagcct
     7381 tgctgtttgt tgccctctct ctaacgcttt cccacaggga gacaggacgg ggcggctgta
     7441 gtgattaagg gttttgcaag gaaaacaagg aaaacaagtt gtacaggctg ttttttattt
     7501 ttcaagcgac ctgaaatcaa cgcttgtaaa tccaagtcgc ttttcaaact gcctgcaacc
     7561 gcaaaatctg ctgcaaccta ccccctcccc cgtgggggag ggctggggag agggcatttt
     7621 ccaagttgcg gcaatctttc ccaaatccct tagtcttcta aaatacaagc cttacggttg
     7681 ttgccaaatg aaacttcttc catacccaaa acttgcgtgt aaaacgcaat ggttcggtca
     7741 atgtcggcaa cggtcagtac cagatggtcg agtgcgctaa tcttcatttc ccgctttctt
     7801 tttcaggcgg cggcagtgcc gtctgaaagc cattatgccc gttttgccgc tttcatattg
     7861 ccaatgaatt tgtcaaacaa atagccgaca tcttgcggac cggggctggc ttcggggtgg
     7921 ccttggaagc agaacacagg tttgtcggtc agctcgatgc cttgcaaagt gttgtcaaac
     7981 aaggatttgt gggtaatgcg tgcgttagcg ggcagggtgt cggcatcaac ggcaaaaccg
     8041 tggttttggc tggtgatgac gactttgccg ctgtccaaat cttgcacagg gtggttcgca
     8101 ccgtggtggc tgaagcgcat tttcagggtt ttcgcgccga tagcgaggct gatgagctgg
     8161 tgtcccaagc agatgccgaa aatcggtttg ccgctttcca tcagtttttg cacggcttcg
     8221 atggcgtagg tgcaaggctc ggggtcgccg gggccgttgg acaggaacac gccgtcggga
     8281 ttgagtgcca acacgtcttg tgcgctcgtt tgggcgggga cgacggtcag gcggcagccg
     8341 cgcgaggcga gcatacgcag gatgttggtt ttcacgccga aatcgtaggc gacgacgtgg
     8401 taaggctgtt cgtcaggggt aacgaaaccc ttgcccaatt cccattcgcc ttccgtccat
     8461 tcgtaagttt ccgtgcagga aacttctttt gccaagtctt tgccgaccat actgccgaac
     8521 gcggcgatga gttcttgcgc tttttcaacc gtagcatccg cgccggtcaa gatcgcgccg
     8581 ccttgcgcgc ctttttcacg caacagcgtg gtcaggcggc gggtgtcgat gtcggcgatg
     8641 gcgacggttt tgttgcgtac caaatagtcg tgcaggcttt cggaggcacg gaagttgctg
     8701 tgcaagagcg gcaggtcgcg gataatcagg ccggcggcgt aaacgctgcg gctttcttca
     8761 tcttcggcgt tggtgccggt gttgccgatg tgggggtagg tgagggtaac gatttgtttg
     8821 cagtaggacg ggtcggtcag gatttcctga tagccggtca tcgaagtatt gaacacgact
     8881 tcgccggaag tcgaaccttc gtaaccgatt gatgtgccgt ggaatacgct gccgtcagcg
     8941 aggacgagga gggcgggggt gctcatgatg ggaatcctgt ttttaataaa attcttgaca
     9001 atgccgtctg aagggacttc ggcagggggc atccggtcgg aaaaatccgg tcaaaaaaaa
     9061 acacgccgcg caagatagac gcgtaacgtg cttttcgggc aagtgcctat accctgaaag
     9121 cagcatattt taagacgaaa catacccgat ttcaagcgca aacgcggcag gcgggctgat
     9181 atgttcgcgc gaaaacggaa atccgtggcg taaaaccgcc cgctctgcgc ccactatgcc
     9241 cgttacgcat ttccccacgt ccgtccggcg aaactatggg aatacccgaa
//
LOCUS       DAWFAD010000075         9268 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWFAD010000075 DAWFAD010000000
VERSION     DAWFAD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.075"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(58..1338)
                     /locus_tag="WGO49_001305"
     CDS             complement(58..1338)
                     /locus_tag="WGO49_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ0306412.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYKTREDKDRMDESKQFYAIEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1560..2180)
                     /locus_tag="WGO49_001306"
     CDS             complement(1560..2180)
                     /locus_tag="WGO49_001306"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ0306413.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAVIDSNLGLDEVRNSIEKVLDGHFGC"
     gene            complement(2240..3235)
                     /gene="mltG"
                     /locus_tag="WGO49_001307"
     CDS             complement(2240..3235)
                     /gene="mltG"
                     /locus_tag="WGO49_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ0306414.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3319..3891
                     /gene="ampD"
                     /locus_tag="WGO49_001308"
     CDS             3319..3891
                     /gene="ampD"
                     /locus_tag="WGO49_001308"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ0306415.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVERLFANRLDPNDHPFFGLIHTLRVSSHFLIKRDGKTVQFVSCDNMAYH
                     AGVSSFGGREKCNAFSIGIELEGCDFEPFAEAQYRSLEALLDAICRHYPVTAVTGHQD
                     IAPGRKTDPGHFFDWRRIREKGFLVDRNAV"
     gene            4087..5373
                     /locus_tag="WGO49_001309"
     CDS             4087..5373
                     /locus_tag="WGO49_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ0306416.1"
                     /translation="MIYIVLFLAVVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5521..8046
                     /gene="ligA"
                     /locus_tag="WGO49_001310"
     CDS             5521..8046
                     /gene="ligA"
                     /locus_tag="WGO49_001310"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ0306417.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMLKADFVALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARQQDGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVVKVNSLAQQHELGFI
                     SRAPRWAVAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKSIEGLQTDKSLPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            8103..>9268
                     /locus_tag="WGO49_001311"
     CDS             8103..>9268
                     /locus_tag="WGO49_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ0306418.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
BASE COUNT         2191 a         2788 c         2330 g         1959 t
ORIGIN      
        1 ctgacagcaa aatgccgcct gaaaacagtt cagacggcat tttaaaccgc aaacggctta
       61 cagcttccat tcgctcagct tggcagcgta agcttccaaa tctgcaatcg gacgggttgc
      121 cacgccgctt tccatcgctg ctttggcggc agccgtagca acgcgcggca gcaggcggga
      181 atcgaacgga gtcggaatca ggtattccgc gccgaattca aatttcttac cgtaagcggc
      241 aaccacttct tcggttactt cttccatcgc caaatccgcc aaagcataca cgcaggcgcg
      301 tttcatttct tcgttgatgg tcgtcgcgcc gacatccaac gcgccacgga agatgaacgg
      361 gaagcacaat acattgttca cttggttcgg gaagtcggag cggccggtac cgataaccac
      421 gtccggacgg gtttctttcg ccagcggcgg caggatttcc ggattcgggt tggccatagc
      481 gaacacgatg ggtttttcgt tcatcgtgtt caacatttcg ggggtcagca ggttcgcgcc
      541 ggacagaccc aagaagatgt ctttgccttt aaccgcatcg gccaatacgc gctggccgtt
      601 gtcttcaatg gcgtagaact gtttagactc atccatgcga tctttgtctt cgcgggtttt
      661 gtaaatcacg ccttttgagt cgcaaacggt cacgttttcg cgtttcaagc ccaaatccag
      721 caattggttc aagcaggcaa tcgcggccgc acccgcacca gagcaaacca aggtcgcttc
      781 ttcgatttta cggccggtaa aacgcagggc gttcaatacg gcagcggcgg taatgatggc
      841 cgtgccgtgc tggtcgtcgt ggaatacggg gattttgcag cgtttgcgta attcgcgttc
      901 gatgtagaaa cactcgggtg ctttgatgtc ttcgaggttg atgccgccga aggtcggctc
      961 taaagcggcg atgatgtcca cgagtttttg cgggtctttt tcatcgattt cgatgtcgaa
     1021 cacgtccaca ccggcgaatt ttttgaacaa tacgccttta ccttccatca cgggtttgcc
     1081 cgccagcgcg ccgatgtcgc ccaagcccaa aacggccgta ccgttggaaa tgacggcgac
     1141 caagttgcct ttggcggtgt atttgtaggc attttgcgga tcggcatgga tttccataca
     1201 aggagcggct acgcccggag agtacgccaa cgccaaatct ttgtcggtcg ccaaagattt
     1261 ggtcggggta acggaaattt tgcccggaac gggtaattcg tggaacttga gggcggcttc
     1321 tttcaatgag ttttccatgt gtcaatcctg ttgcgagaaa aagatttgaa tcggactgtc
     1381 cgaaacgctt tcgggtcatc atgttacggg cggttcgtta atattgccga cgcatcctgc
     1441 cttgtaaccg agcgtaattc tatcataccg aaagtttggc gcataccgcc attttcacct
     1501 gcggcactgc cgtttgattt tcggcgcggg cggatgcgct tgtttcaata tttgccgcat
     1561 taacagccga aatgtccgtc caacactttt tctatgctgt ttctgacttc atccaagccg
     1621 aggttactgt cgataacggc gtaacgttcc ggacaggcgg cggcacggtt cagataaacg
     1681 ctgcgcacac gcatgaagaa atctgcctgt tcctgctcga aacggtcttt ctcgcgcgtc
     1741 tgcccgatac gcgccatcga cacttccagc ggcacatcca acagcagggt caaatcgggg
     1801 cgcaaaccgc cctgcaccca atgttccaaa atttcaatgt cttcagacgg catcccccgc
     1861 ccgccgccct gataggcgaa ggttgcatcg gtaaaacggt cggacacgac atggatgccg
     1921 tctgaaagtg caggcaatat cacgtcttca atatgctgca ttctggcggc gaacatcatc
     1981 agtgtttccg cacgcaaacc ggctttggtt tcagggttga gcaggatttc gcgcaaggcc
     2041 tcaccgaccg gcgttccgcc cggctcgcgc gtgaacagca cgggcagccc cctccgttca
     2101 aaccatgctt tgatgacggc aaggttggtg gattttccgg ctccgtctat gccgtccaaa
     2161 gtgatgaatt gcggtttcat gcttgcagtt ccggtaaggg tatgccgtct gaaaacacaa
     2221 acttttcaga cggcatggtt tattttttca aaatatattt gcggacggcg gcattgtgtt
     2281 cggtcaaatc atggctgaac tggctcaagc ccgtaccgtc cattttagaa acaaaataaa
     2341 ggtatttttc gccagacgga tgggcggcgg catcaagcgc ggccttgccg ggcagcgcaa
     2401 tcggggttgg cggcagaccg ccgcgcgtgt aggtgttgta cggcgtgtcg cggcgcaggt
     2461 cggctttacg gattttgccc ttgtatgccg cacccatgcc gtaaatcacg gacgggtcgg
     2521 tttgcaggcg cataccgatt ttcaggcggt tgacgaagac ggaagcgaca tggtcgcggt
     2581 cggcttcatg ccccgtttcc ttttcgacca ggctcgccat aatcagcatt tcataaggat
     2641 ttttataagg cagcccgtcc tgcctgcttt cccatgcctc attcaggcgg cgttgcatcg
     2701 ccttgtaggc ggtttggtaa atccgtaaat cgctgccgcc cgcatcgatt tcgtagctgt
     2761 cggggaaaaa ctgcccttca ggattgccgc tgaaggcatc aggggcaact tccgccatca
     2821 gtttttcatt gctccagcct ttggtgtcgt gttcgatgtc gggcgttgcg tcgatgactt
     2881 tcctcatatg cgaaaaacgc gaaccttcga taatctgcac ggtaacggaa tccggcctgc
     2941 cgccgcgcat tttctgcaag atatcccaag cagacacttc cgaaggcagt ctgtacgtcc
     3001 ccgtatgcag cctgttgtgc acacccaaaa cgtaggccgc cgccgtcaaa acatgcctgc
     3061 tgaacacgat gcggtcttcg gcaagtttcc tgccgaccga cgaaataccc tggtttttgg
     3121 caattttaat tcggtatgcc ctgccgttgt ctttagggac gaaaagcagc gcggcgaaaa
     3181 cggctgccga tacggtcaaa aaaacggcag accatttcaa caattttctc aacatggtag
     3241 gattcccagc attcggaata cgtcttaaaa ttcgggcgac agtataaccc aaattgcccg
     3301 ccggacagga agaacatcat ggacaatcac gccgaagcac actggcaaaa cggctggctt
     3361 caaagcatac gccatacccc gtcacccaat ttcagcccga gggaaacggg ggaaacggtt
     3421 tccctgattg tgttgcacaa catttcgctg ccgccgttcg aatatggtac ggatgcggtt
     3481 gagaggcttt ttgccaatcg cctagacccg aacgaccacc cgtttttcgg attgatacac
     3541 actttgcgcg tatccagcca tttcttaatc aaacgcgacg gcaaaacggt acagttcgtt
     3601 tcctgcgaca atatggcata ccatgccggc gtatcttcat tcggcggcag ggaaaaatgc
     3661 aacgcatttt caatcggcat cgaactggaa ggctgcgatt tcgaaccgtt tgccgaagcg
     3721 caataccgtt cgctcgaagc cttgttggac gcaatctgcc gccactaccc cgtcacggcg
     3781 gtaacggggc accaagacat cgcgcccggc cgcaaaaccg accccggcca ttttttcgac
     3841 tggcggcgga tacgggagaa agggtttctc gtggacagaa atgccgtctg aaaaccacag
     3901 atttcagacg gcatttcgga aaatttccgc ttatttcgac acactgctgt cagaccctgc
     3961 aattttcgtt ataattcaaa cgttataatc ggccgttccg tcagactgcc ggaaacgcaa
     4021 gcccgattta accgcagtac ccgcactttg cgtatcctgc atcccgaaat ttaacaagtg
     4081 attgccatga tttacatcgt actgtttcta gctgtcgtcc tcgccgttgt cgcctacaac
     4141 atgtatcagg aaaaccaata ccgcaaaaaa gtgcgcgacc agttcggaca ctccgacaaa
     4201 gatgccctgc tcaacagcaa aaccagccat gtccgcgacg gcaaaccgtc cggcgggtca
     4261 gtcatgatgc cgaaacccca accggcggtc aaaaaaacgg caaaacccca agaccccgcc
     4321 atgcgcaacc tgcaagaaca ggatgccgtc tacatcgcca agcagaaaca ggcaaaagcc
     4381 tccccgttca aaaccgaaat cgaaaccgcc ttggaagaaa gcggcattat cggcaactcc
     4441 gcccacaccg tttccgaacc ccaaaccgga cattccgcac cgaaacctgc cgacgcgccg
     4501 gcaaaacctg cacccgttcc gcaaacacct gcaaaaccgc tgattacgct caaagaactg
     4561 tcaaaagtcg aattaccctg gtttgacgtg cgcttcgact tcatctccta tatcgcgctg
     4621 accgaagcca aagaactgca cgcactgccg cgcctttcca accgctgccg ctaccagatt
     4681 gtcggctgca ccatggacga ccatttccag attgccgaac ccatcccggg catccgctat
     4741 caggcattta tcgtgggtat tcaggcagtc agccgcaacg gacttgcctc gcaggaagaa
     4801 ctctccgcat tcaaccgcca ggtggacgca ttcgcacaaa gcatgggcgg tcagacgctg
     4861 cacaccgacc ttgccgcctt tatcgaagtg gcttccgcac tggacgcatt ctgcgcgcgc
     4921 gtcgaccaga ccatcgccat ccatttggtt tccccgacca gcatcagcgg cgtagaactg
     4981 cgttccgccg taacgggcgt gggtttcgtt ttggaagacg acggcgcgtt ccactatacc
     5041 gacacgtcgg gctcgaccat gttctccatc tgctcgctca acaacgagcc gtttaccaat
     5101 gcccttttgg acaaccagtc ctacaaaggc ttcagtatgc tgctcgacat cccgcactct
     5161 ccggcaggcg aaaaaacctt cgacgatttg tttatggatt tggcggtacg cctgtccggc
     5221 cagttgaacc tgaatctggt caacgacaaa atggaagaag tttcgaccca atggctcaaa
     5281 gacgtgcgca cttatgtatt ggctcgtcag tccgagatgc tcaaagtcgg tatcgaaccg
     5341 ggcggcaaaa ccgcattgcg cctgttctcc taaaatcgaa tgcaatcaaa gcggatatgt
     5401 cgttacggca tatccgcttt ttttcgggta aaattcagct gttttcaacc tatcattttt
     5461 cagacgacct ttgcccgttt catcccgtat cagacaaagg tcgtctgaaa accttctctc
     5521 atgaatccaa actcaaaaca taacactaac tttaccaatc ttcttaaacc gtctgatagt
     5581 gatataaaac aatttgccgc acaacacatc cgccacctca ccgacctgct caaccgctat
     5641 gcctacgaat actacaccct cgacgcgccc agcgtacccg atgccgaata cgacaaatta
     5701 ttccgcgagc tcgaagcgtt ggaattaaac catcccgagc tcaaactgcc cgacagtccg
     5761 acccagcgcg tcggcggcga gcctttggcg ggatttgccg aagtgcgcca cgaagtgccg
     5821 atgctgtcgc tgaccaacgc tttttccccg caagatgaaa acggcgtgtt cgaccatgcc
     5881 gaaatgtacg ctttcgacca acgcgtgcgc gacggtttgg acggcggcaa acccgaatac
     5941 gttatcgaac ccaaattcga cggcctcgcc atcagcctgc tctaccgcga cggcgtattg
     6001 gtgcaggcgg caacgcgcgg cgacggcacg acgggcgaag acgttaccca aaacatcaaa
     6061 accgtatcca acatcccctt gcggctgcac ggcgaaaata cgcccgaact catcgaggta
     6121 cgcggcgaag tgctgatgct caaagccgat tttgtcgccc tcaataaaag acaagccgaa
     6181 aacgggcaaa aaccctttgc caatccgcgc aacgccgccg ccggcagcct acgccaactc
     6241 gattcgcgca tcaccgcgca acgcaaactg cactttttcc cctactccgt cgcccgccag
     6301 caagatggtt tcgtcgcgga agaacacatc caggaactcg cctatttcca agcactcggc
     6361 ttcagtctgc ccaatggcaa tttcggctgt ttcaaaaata tcgacgaagt attggcgttt
     6421 tacgaacaca tgcagcaaaa acgccccgaa ctgccctacg aaatcgacgg catggtggtt
     6481 aaagtcaaca gcttggcgca acagcacgaa ctcggcttca tctcccgcgc gccgcgttgg
     6541 gcggttgccc acaaattccc tgccgaagaa gccctgacca ttgtcgaagc gattgacgtg
     6601 caaatcggca ggacgggcgc ggtaacgccg gttgcccgat tacagcccgt attcgtcggt
     6661 ggcgtaactg ttaccaacgc caccctgcac aatcaggacg aagtttcccg aaaagacgtg
     6721 cgcgtcggcg ataccgtcgt cgtgcgccgc gccggagacg tgattcccga agtcgtgcgt
     6781 gtgatttttg aacgccgccc gatgcaggaa accgccgttg ctgtttcaga cggcatcggg
     6841 catcggcagg acgatttgtt tgccgaagca ccggctgcaa accaaaccca atccgttccg
     6901 ctctacaagc cctaccgcct gccgacccat tgccccatct gccgcagcga aatcgaacgc
     6961 gaagaaggcg aagccgtcgc ccgatgcagc ggcggtatgc tttgtcaggc acaacgcgcg
     7021 caaggcttaa tccacttcgc ctcgcgcaaa gccatggaca tcgacggctt gggtgaaaaa
     7081 caaatcgaac agcttgtcgc ccaagacctc gtccgccact tcgccgacct ctaccgcctc
     7141 gatattccga ccctgcaaaa aatgaaggaa acggcggaca aaaccgttgc cgaatcggat
     7201 caaatgccgt ctgaaggcag ttcggtcggt gcgtcgggca aacataaaaa acaaccgacc
     7261 aagtgggcgg aaaacatcct cgcaggcata gaatccggca aaacgcccga actcgcgcgc
     7321 ttcctgttcg cgctcggcat ccgccacgtc ggcgaacgca ccgccaaaac gctggcacag
     7381 gcattcggca cattggaacg cgtccgccgc gcccccgaac ccgtcctcgc ctgcctgccc
     7441 gacatcggta cggtagtggc gcgttccatt gcccacttct tcgcccaagc cgaacagcag
     7501 gcgatgatag acgagctgct cgccgcaggc gttgccccgc aagcccaagc cgtcagcctg
     7561 cctgccgcac aatatgccga accgcaacgc tggattgccc gcctgcccgg tttcaaaatt
     7621 agcgaaaaca aagcccaagc cttatgggaa ctcgccggca aaagtataga gggtctgcaa
     7681 accgacaaat ccctccccgc cgactggcaa gcatggcgca gcgaaccaca aaacgccgcc
     7741 ctactcgaaa acctgaaaac cttcttcgcg caaatgccgt ctgaagacga ggcggcgcaa
     7801 ggttcagacg gcatcaataa agccgtagcg ggcaaaacct tcgtgttaac tggcacactg
     7861 cccaccctca aacgcgacca agcccaatcc ctaatcgaag ccgcaggcgg caaagtttcc
     7921 ggcagcgtgt ccaaaaaaac cgattacgtc gtcgccggag aagccgcggg cagcaagctg
     7981 gaaaaagcca atgccttggg cgtttccgtc ctcagcgaag ccgaactgct taccctgctc
     8041 ggctgaaccc atgccgtctg aaccgccttc agacggcatt gcccactttt ccgaaccaca
     8101 ccatgcacac catttccttt ccaaaccgca cccgactcac cgccctgccg cccctgtcgc
     8161 tttacatcca catcccgtgg tgcatcaaaa aatgcccgta ttgcgacttc aattcccaca
     8221 gcctgaaaaa cggattgccc gaagccgcct atatcgacgc gctgctgacc gatttgcagc
     8281 ttgaattgcc caatatttgg ggcaggccgg tggaaacgat ctttttcggc ggcggtacgc
     8341 ccagcctgtt tcaggcggaa tcgattgacc gtttgttaag cggcgtgcgt tcgctgttgc
     8401 gcttgcagcc ggaagcggaa attaccttgg aagccaatcc ggggacattt gaaatcgaga
     8461 agtttcaagg atttaaagac gcgggtatta cgcggctttc tatcggcgta cagagtttca
     8521 acgacgatat gctctcaagg ctggggcgcg tccacaacgg taaggaagcc ttaaccgcta
     8581 tcgataccgc cttgaaatta tttgataaag tcaatatcga cttgatgtat gccctgccga
     8641 accaaaccgt tcagacggca ttggacgacg tacaaaccgc tatcgctacg ggcgcgaccc
     8701 acatcagcgc gtatcatctg acgatggaac cgaacacgcc gttcggtcat acgccgccga
     8761 aaggtttgcc gcaagacgaa gccgccctcg acatcgaaga cgcggtacac ggcgcgctgg
     8821 aaggcgcggg ttttgtccac tacgaaacat cggcttttgc gaaaccagcc atgcagtgcc
     8881 gccacaattt gaactactgg cagttcggcg attatttagg cataggcgcg ggcgcgcacg
     8941 gcaaaatttc ctatcccgac cgcatcgagc gcaccgtccg ccgccgccac cccaacgact
     9001 acctcgcctt aatgcaaagc caaccgagtg aagccgtcga acgcaaaacc gtcgctgccg
     9061 aagatttgcc gttcgaattc atgatgaacg ccctgcgcct gaccgacggc gtacccgccg
     9121 cgatgttgca ggagcgcacg ggcgtaccga gtgccaaaat catggcgcaa atcgaaacgg
     9181 caaggcaaaa aggcctgctg gaaaccgacc ctgccgtatt ccgcccgacc gaaaaaggac
     9241 gcttgttttt aaacgatttg ctgcagtg
//
LOCUS       DAWFAD010000076         9268 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWFAD010000076 DAWFAD010000000
VERSION     DAWFAD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.076"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(100..1017)
                     /gene="thrB"
                     /locus_tag="WGO49_001312"
     CDS             complement(100..1017)
                     /gene="thrB"
                     /locus_tag="WGO49_001312"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ0306419.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLVAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(1054..1782)
                     /gene="ubiG"
                     /locus_tag="WGO49_001313"
     CDS             complement(1054..1782)
                     /gene="ubiG"
                     /locus_tag="WGO49_001313"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ0306420.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            1934..3175
                     /locus_tag="WGO49_001314"
     CDS             1934..3175
                     /locus_tag="WGO49_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ0306421.1"
                     /translation="MPTKTPSLFGCAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGIGNALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPAGTGYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWMGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            3233..4287
                     /locus_tag="WGO49_001315"
                     /pseudo
     CDS             3233..4287
                     /locus_tag="WGO49_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            complement(4354..6006)
                     /locus_tag="WGO49_001316"
     CDS             complement(4354..6006)
                     /locus_tag="WGO49_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0306422.1"
                     /translation="MVAYVFLFLFVTAALVLIIRSHYRWTYFFASALFVFLAGGMLML
                     TAQWQRALNFASVWFVVLILFHRLKIHYYKQPLLISDFLLIADWRNWETLFHYKEAVI
                     GMAGLLALAAYAVFGWSGADSLGVPWRWAGAALFAAAFVSMWYFSKHPGAVKTWLDSL
                     PDDGRDVFLNLPMSCRAVFFQVPVFEGDGEAFAKQMPSETQPCGMSDEKPDIVVTLME
                     STLDPHCFDFAAAKIPDLKMFGRQEDTVFSSPLRVHTFGGATWKSEFAFLAGVPSTDF
                     GALASGVFYSVVPHLQTGLVRNLREHGYFCVALSPFTKGNYNAKAAYDHFGFNLMFQP
                     QDLGYPAPMGKNLWHISSEEMMQYARMILEKRHPDLENVRQPMFVYVLTMKEHGPYRT
                     DTDNVFDLDAPDLNAKTVSALNDYIGRIADLDKAVESFDRYLHERGKPFVFGYFGDHQ
                     VPFEGVSVRKKWDYAQPDYVTQFAVRSNIAGGFVQRQDFLDLAFAGGVLMEAAGLEAK
                     DGFMRANMAMRGLCGGGLEDCPNRKLVGDYRNYLYDVLKIAS"
     gene            6176..6749
                     /locus_tag="WGO49_001317"
                     /pseudo
     CDS             6176..6749
                     /locus_tag="WGO49_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            6746..7309
                     /gene="gmhB"
                     /locus_tag="WGO49_001318"
     CDS             6746..7309
                     /gene="gmhB"
                     /locus_tag="WGO49_001318"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ0306423.1"
                     /translation="MKLIILDRDGVINQDRDNFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            7339..8082
                     /locus_tag="WGO49_001319"
     CDS             7339..8082
                     /locus_tag="WGO49_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0306424.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            8079..8771
                     /locus_tag="WGO49_001320"
     CDS             8079..8771
                     /locus_tag="WGO49_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0306425.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPEHIWFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPAPSSLTSAKTFWGV
                     CRKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHADHSRAFWALTRRFAPYTPEAKQW
                     LKIHGRELFALG"
     gene            8882..9067
                     /locus_tag="WGO49_001321"
     CDS             8882..9067
                     /locus_tag="WGO49_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306426.1"
                     /translation="MPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRPSETRNP
                     PGPTCGMKRLSWREVFY"
BASE COUNT         2318 a         2861 c         2164 g         1925 t
ORIGIN      
        1 ggaaaatacc cggctatcgt ctcattttcg ttttaatcag ccataaaaat gccgtctgaa
       61 accctttcag acggcatttc tgtcaaacgc cggacgcact caatccaaac tcaacagcag
      121 gttgcggaac gcgttcgggt ctttgataaa cgtcatctcg cccgcctgcg gaaaatgaaa
      181 atccaacagg cgcgacaccc aaaaacggat gcagccggcg cgttgggcgg tcggaaaata
      241 cgccttttct tcggcactca aggggcgcac gccctcataa ccgccgataa acgccttttt
      301 caacgcctca tccaacttat tgtccgccgt ccttgcccaa tcgttgaccg caatcgccaa
      361 gtcatacata aaattgcccc ggcaggcata ataaaaatcg ataaagcccg acacctgacc
      421 gccgtcaagc aacacattgt ctttaaacaa atcggcgtgg atgatgcccg aaggcagatg
      481 attgccgaga ttgtccttca gcgcatcgat ttcggaacac agcagcgcag catcgtcttg
      541 cgacaggacg ggcagcagcc gggcgcacgc ctccgtccac cacgcatcgt aacgcgggtt
      601 ttccatttcc aaagggaaat cggcggcgac aaggtgcatt ttggccaaca tcgcaccggt
      661 atggaaacac tgctccgccg tcggcagcgc agtatccgaa cctttcaggc aggcaaccaa
      721 gcaggcgggc ttgcccgcca aaacggaatc aagccggccg tctttgcgcg caaccggcgc
      781 ggcaaccgcc acgcccttca tactcaaatg ccggttaagc tccagaaaaa acggcagctc
      841 ttcctgtttc aacacttcaa acacggtcag cacataacgt cccgaagtcg tcgtcagaaa
      901 ataattgctg ttggtaatcc cctgcgcgat gccctgcagg gaaacaaatt cccccaaatc
      961 gtaaccgctc aggaagccgc gcatttcatc atcggaaaca ctggtataga cagacatatc
     1021 aactcacttg ctcaaaaacg ggcaaacccg ccgtcagaac gccggacggc aggcaaacat
     1081 ataattcaca tcggtcgaat cgcacagggc ataagtttgc gacaacacat ggtaagtcat
     1141 acccttcgta tccgccacat ccaagcctgc ctgacggcac attcgcgcca gctcggcagg
     1201 tgcgatgaat tttttccagt cgtgcgtgcc tttggggaca aacttcaaca gatattccgc
     1261 cgccacaatc agatgcaggt acgatttcgg gtttttattg atggtagaaa aaaacaccat
     1321 gccgtccggt ttgaccagat tggcacaagc acgcacgatg gcggcgggat cggggacgtg
     1381 ttccatcatt tccatgcacg ttaccacatc gaacgagtgc ggttccgcct cggcaaggtc
     1441 ttccacgcgg atacattcgt attcgatatc ggcgacattg ttcaaagccg cgtgcaggcg
     1501 ggcggtttcc agcgactgct ccgccatgtc gatacccttt acaaacgccg cgccgcgccg
     1561 cgccatgctt tccgccagga tgccgccgcc gcagcccaca tccaaaaccc gtttgccgca
     1621 caaatccgcg tgtccgtcga tataatccag ccgcagcgga ttgatgtcgt gcaaggtttt
     1681 gaactcgccc gacttgtccc accatttgtc ggcaatccgg ctgaatttgg cgatttcccc
     1741 ctcatcgaca ttatattttt tgtcggacat tttccctccc atctgacgaa ccgcccactc
     1801 caaaacccaa gattatacta tggaacgcaa tgccgtctga aagccttttc gggcggcgcg
     1861 gcgcaaatac cttacaaatc cttacacttt acggcataat ggcggctcgc tttttctggc
     1921 agaaagacaa aatatgccca ccaaaacccc ttcactgttc ggctgcgcga tgattatcgc
     1981 cggcacggtc atcggcgcag gtatgctcgc caacccgacc gccacatccg gcgtatggtt
     2041 taccggctcg ctggccgtgt tgctgtacac ctggttttcc atgctctcca gcggcctgat
     2101 gattttggaa gtcaacaccc actaccccca cggcgcgagc ttcgacacca tggttaaaga
     2161 cctgctcgga cgcggctgga acatcatcaa cggtatcgcc gtcgccttcg ttttatacct
     2221 gcttacttac gcttatatct tcgtcggcgg cgacctgacc gccaaaggca tcggcaacgc
     2281 cttgggcggc aatgtttcgc ttaccgtcgg acaactcgtc ttcttcggca tcctcgcctt
     2341 ttgcgtatgg gcatccgcac gcttggtcga ccgattcacc agcgtcctca tcggcggcat
     2401 ggtattaacc tttatttggg caaccggcgg cctgattgcc gatgccaaaa tgcctgtttt
     2461 gtttgacacc caagcccctg ccggcaccgg ctactggatt tacgccgcca ccgccctgcc
     2521 cgtctgcctc gcttccttcg gcttccacgg caacgtttcc agcctgctca aatactttaa
     2581 aggcgacgcg cccaaagtgg ctaaatccat ctggatgggc acactgattg cgctggtaat
     2641 ttacgtcctc tggcaaaccg ccatccaagg caacctgccg cgcaacgagt tcgcccccgt
     2701 gattgccgcc gaagggcaag tctccgtcct catcgaaacc ctgtccaaat tcgcccaaac
     2761 cggcaatatg gacaaaatat tgtccctgtt ttcctatatg gcgatcgcca cctcgttttt
     2821 aggcgtaacg ctcggactct tcgactacat cgcagacatc ttcaaatgga acgacagcgt
     2881 gtccggccgc accaaaaccg ccgcgctgac cttcctgccg cccctgattt cctgcctgct
     2941 cttccccacc ggcttcgtta ccgccatcgg ctacgtcggc ctggcggcaa ccgtctggac
     3001 aggcatcatc cccgccatgc tgctctaccg ttcgcgcaaa aaattcggcg caggcaaaac
     3061 ctataaagtt tacggcggct tgtggctgat ggtttgggtc ttccttttcg gcatcgtcaa
     3121 catcgccgca caggtattga gccaaatgga actcgtcccc gtatttaaag gataaaggca
     3181 aaatgccgtc tgaagccctg ttcagacagc cttacgacat tcggaaaatc atatggaact
     3241 caaaatagac attgcaacca acaacttcaa acacggcggc ggcacggaac gctacacatt
     3301 ggatttggta aagggtctga acagacaaaa catcacaccg gccgtttatg cgacgaaatt
     3361 tgatcacggc attcctgaat acgccatgat cgaaccccat cttgtcgatc aacaccggac
     3421 gctgaaaaaa ctacgctcat tcctcttttc aagtcggctc gctcaaacca gaaaaaacag
     3481 tgccgccaaa ctgattgcct gccaccacgc cgattacgcc gacctcctca tctgcggcgg
     3541 cacacacttg ggctacctgc accatatggc gcaaaaaccg aacctgctcg accgtctcgc
     3601 catacgccgc aaccgcagca actacgccac cgccaaactg attatggcgc attcccatat
     3661 gatgcggcgc gaactggtcg gactgtacgg cgttccccct gaaagaatcc aagtcgcccc
     3721 ccccccgcag atacggaacg cttctttcca caacccggag aaactgccga cctgcgcgcc
     3781 aaatacggct ttgccgacca tgaaaccgtt ttcctgttcc catcgaccgg ccacacgcgc
     3841 aaaggtctgg aactgcttgc cgactttttc gaacataccg gcctgcccgt caagcttgcc
     3901 gttgtcggct ccccgctccc ccgccctatg aaaaacgtcg tcggactggg cttctgcacc
     3961 gatatgcccg aactctaccg cgccgccgac tttaccatta tggcctccct gtatgaaccc
     4021 ttcgggctgg tcggtgtcga atccgtccta tgcggcacac gcgtcgtcct ctccgaaaac
     4081 atggcatgta cagaggtcat gaacgaagaa gccggattct ttttctcacg ccaaaacccg
     4141 gaaaccctgg cgcaagccgt tgcccaagcc gtcagcctta aaaaacaggg cggacaccgc
     4201 ctgtccgacc cgatgcgtgc actgaactac aacccgtccc tgtcgcacca catcgaccga
     4261 ctgaccgaca tactggcatc cgtataacaa aatgccgtct gaacgagttt tcagacggca
     4321 tttttgcaac cgttgcagcc gtgcggcaaa aagctaactg gcaattttca aaacgtcgta
     4381 cagatagttc cgatagtctc cgaccagttt ccggttcggg cagtcttcca accctccgcc
     4441 gcacaaaccg cgcatcgcca tattcgcacg catgaagccg tctttggctt ccagaccagc
     4501 cgcctccatc agcacgccgc ctgcaaaggc aaggtcgagg aaatcctgcc gctgtacgaa
     4561 tccgccggca atattgctcc tgacggcaaa ctgcgttaca taatccggct gcgcgtaatc
     4621 ccatttcttc ctgacggaca cgccctcaaa cggtacctga tgatcgccga aataaccgaa
     4681 aacaaagggt ttgccgcgtt cgtgcagata acggtcgaaa ctttccaccg ctttgtccaa
     4741 atcggcaatg cgcccgatgt agtcgttaag cgcggatacg gttttcgcat tcaaatcagg
     4801 tgcatccaaa tcaaacacat tgtcggtatc cgtccgatac ggcccgtgct ccttcatggt
     4861 cagcacatat acgaacatcg gctgccgcac gttttccaaa tcgggatggc gtttttccaa
     4921 aatcatccgc gcatactgca tcatttcctc actggaaata tgccacaggt ttttgcccat
     4981 cggcgcggga tagcccaaat cttgcggctg gaacatcaga ttaaagccga aatggtcata
     5041 tgctgccttg gcattgtagt tgcccttggt aaacggcgag agtgccacgc aaaaataacc
     5101 gtgttcgcgc aggttgcgga ccaaaccggt ctgcaaatgc ggcacgaccg agtaaaacac
     5161 gccacttgcc aacgcgccga aatccgtgga cggaacaccc gccaaaaacg caaattcgga
     5221 cttccacgtt gcgccgccga aagtatgcac gcgcaaaggt gaggaaaata cggtatcttc
     5281 ctgccgcccg aacattttca aatcgggaat cttggcggca gcaaagtcga aacagtgcgg
     5341 atcgagcgtc gattccatca gggtaacgac aatatcgggt ttttcatccg acatgccgca
     5401 cggctgggtt tcagacggca tctgtttagc aaacgcttcc ccgtcgccct cgaacacggg
     5461 tacttggaaa aacaccgccc gacaggacat cggcaggttc aaaaacacgt cgcgcccgtc
     5521 gtccggcagc gagtccagcc acgtctttac cgcgcccgga tgtttggaaa aataccacat
     5581 cgacacgaac gccgccgcaa acaacgccgc gcccgcccac cgccacggca cacccaaaga
     5641 atccgcaccg ctccagccga aaaccgcata tgccgccaaa gccagcagcc ccgccatacc
     5701 gataaccgct tccttataat gaaacagcgt ttcccaattc cgccagtcgg caatcagcaa
     5761 aaagtcggaa atcaacagcg gctgtttgta ataatggatt ttcagcctgt ggaacagtat
     5821 cagcaccaca aaccagaccg aagcgaaatt caaggcgcgc tgccactgag ccgtcaacat
     5881 cagcataccg cccgccaaaa agacaaacag cgcggatgcg aaaaaatacg tccagcggta
     5941 gtgcgacctg ataatcagca ccaacgccgc cgttacaaac aaaaatagga aaacataggc
     6001 aaccatcccg tcccactcct caaaacggca tacctgagcc gttgcgaatt attatcgaaa
     6061 gtgtgcatta taatgcagca caggcgcatc actgccgata tttcagtata attattcgag
     6121 atggaatcac tgaaaatggt gaagttacaa tgagcccttt tagaggaact aaaaagtgaa
     6181 ttataaaata tacgatggtg caaagattat taaaaaagaa aatttatcaa ttggggatta
     6241 ttctcaaatt gatgattttg tattttttaa ttgtggagag aaaagtattt taggaagttt
     6301 tgttcatata tcatcattta ccagtattat tggtggtgga gaattttata tggatcattt
     6361 ttcaggatta agcgcagggt gtagaattat taccggtagc gatgatttta tggggggggg
     6421 gggggttaac caatccaaca gtcccatcta aatatactaa tgttaaaaaa tctagtgtta
     6481 gaattggaaa acatgcaatt ttaggaacaa atgtaacagt attaccaggc gttactattg
     6541 gtgatggggt tgctgttgga gcaggggcta tagtgagaaa aaatcttgag ccgtggacta
     6601 tttatgtcgg ggtagattgt aaaccactga agcgcagatc acaagataaa atattggagt
     6661 tagagaaaca tcttgtagca gaatttaatc tagatttatg tcctataatc ccatccccga
     6721 acctgcccaa accaaaggaa acgcgatgaa actcatcatt ctcgaccgcg acggcgtcat
     6781 caatcaggac cgcgacaact tcgtcaaatc cgttgacgag tggatacctg tcgaaggcag
     6841 catggatgcg gtggcatttc tgacgcaggc aggctacacc gtcgccgttg ccaccaacca
     6901 atccggcatc gggcgcaaat attttaccgt tcaaaacctt accgaaatgc acgccaaaat
     6961 gcaccgcctc gtccgtcagg caggcggcga aatcaacggc atctggttct gcccgcacac
     7021 cgatgccgac aactgcaact gccgcaagcc caaaccgggt atgattgaag acatcatcgg
     7081 acgcttcaac gcccaagcct cggaaacctg gctggtcggc gacagcctgc gcgatttgca
     7141 ggcaatcgat gccgtcggcg gaaaacccgc gctggttctg accggaaaag gcaaaaaaac
     7201 gctctcccaa cacggacacg aattacccga acacacacag gttttcgata ccttgctcga
     7261 tttctcacaa tacatcatgc aggaaaacgc cgcaccgcaa gccgactgaa cataccgcat
     7321 ttccgacaag gcaaaaccat gctcatcatc cgcaacctga tttactggct gatactctgt
     7381 tccaccctga ttttcctctt tccctttatg ctgctcgcct cgcctttccg ggacggggcg
     7441 cacaagatgg cgcgggtctg ggtcaaaatc ctcaacctct cgctcaaaca catcgtcggg
     7501 ctcaaatacc gcatcatcgg cgcggaaaac atccccgacc gccccgccgt catctgcgcc
     7561 aaacaccaaa gcggctggga aacgctcgcc cttcaggaca tttttccgcc gcaggtttac
     7621 gttgccaaac gcgagttgtt caaaatcccc tttttcggct ggggcttgaa actggtcaaa
     7681 accataggca tagaccgcaa caaccgccgc gaagccaacg agcagctcat aaaacagggg
     7741 ttggcgcgca aaaacgaagg ctattggatt accattttcc ccgaaggcac gcgccttgcg
     7801 cccggaaaac gcggcaaata caaactcggc ggcgcgcgca tggcgaaaat gtttgagatg
     7861 gacatcgtcc ccgtcgccct caacagcggc gaattttggc cgaaaaactc ctttctgaaa
     7921 tatccggggg aaatcaccgt cgtcatctgt ccgaccatcc cgcacgcaag cggcagcgaa
     7981 gccgaattga tggaaaaatg cgaacatctc atcgaaacgc aacaaccgct tatttccggc
     8041 gcaggcccgt ttgccgccaa aatgccgtct gaaaccgcat gaccgccttt gtccacaccc
     8101 tttcagacgg catggaactg accgtcgaaa tcaagcgccg tgccaagaaa aacctgatta
     8161 tccgccccgc cggcacacat accgtccgca tcagcgtccc accctgcttc tccgtctccg
     8221 ctctaaaccg ctggctgtat gaaaacgaag ccgtcctgcg gcaaacactg gcgaaaacac
     8281 cgccgccgca aactgccgaa aaccggctgc ccgaacatat ctggttccac ggcagacagc
     8341 ttgccctcac cgcccatcaa gacacgcaaa tcctgctgat gccgtctgaa atccgtgttc
     8401 ccgaaggcgc acccgaaaaa cagcttgcgc tgctgcggga ctttttggaa cggcaggcgc
     8461 acagttacct gattccccgc ctcgaacgcc acgcccgcac cacacaactg ttccccgccc
     8521 cctcctcgct gacctctgcc aaaaccttct ggggcgtgtg ccgcaaaacc acaggcatac
     8581 gcttcaactg gcggctagtc ggcgcaccgg aatacgttgc cgactatgtc tgcatacacg
     8641 aactctgcca cctcgcccac gccgaccaca gccgcgcgtt ctgggcactg acccgccgct
     8701 tcgcacccta cacgcccgaa gcgaaacagt ggctcaaaat ccacggcagg gaacttttcg
     8761 ccttaggctg acgcggcccg ggcaggcatc cgccaattca gacctcctcc caaacggaca
     8821 tccggaaagc agcatcacag ccggcgacac ataccgctgc cgccgctttc agacggcatc
     8881 cgtgccggaa caggcacgcg cccgcccgat tcaaaccgcg atgacgcttt gccgccggtt
     8941 cggggcagga tggcggcaca cacgccgtct gccgcgtttc atttcacacc gcccttccga
     9001 aacccgaaac ccgcccggtc cgacgtgcgg tatgaaacgc ttaagctggc gcgaagtctt
     9061 ttactgattt gcccgcgaaa atgccgtctg aaaggttttc ggacggcatt tttttgcgtt
     9121 tcccaggatg gcggcggatt cgtaaaaggc ggtcagggtg gattgtagga tgggttgaga
     9181 cctgccgaat ccgccgcatc tgccaaatct accgccgtca ttcctacgaa agtgggaatc
     9241 tagaacgcgg ggttaagaaa acctgcat
//
LOCUS       DAWFAD010000077         9153 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWFAD010000077 DAWFAD010000000
VERSION     DAWFAD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9153
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.077"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..210
                     /locus_tag="WGO49_001322"
     CDS             <1..210
                     /locus_tag="WGO49_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEZ0306427.1"
                     /translation="WLDTGTHASLLEAASFVETIENIQNLQVACLEEIAWRNGWLSSE
                     EIRNLAKPMCKNEYGRYLLRLVGKD"
     gene            265..804
                     /gene="rfbC"
                     /locus_tag="WGO49_001323"
     CDS             265..804
                     /gene="rfbC"
                     /locus_tag="WGO49_001323"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0306428.1"
                     /translation="MNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERTF
                     VQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVLS
                     ADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLGE
                     PSLSAKDLAGKMLAEAATF"
     gene            966..3014
                     /locus_tag="WGO49_001324"
     CDS             966..3014
                     /locus_tag="WGO49_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ0306429.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPEINRL
                     VQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMGIYFNAEAPSRLEHILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGNSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYH
                     NLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQY
                     ADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGCFAKKLGKIKQLYRSFK
                     "
     gene            3151..4410
                     /locus_tag="WGO49_001325"
     CDS             3151..4410
                     /locus_tag="WGO49_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide modification protein
                     LipB"
                     /protein_id="HEZ0306430.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            4777..6183
                     /locus_tag="WGO49_001326"
     CDS             4777..6183
                     /locus_tag="WGO49_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0306431.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     DHLLQNGHPEQALPWLEAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAETQAHAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            6401..7615
                     /gene="gltS"
                     /locus_tag="WGO49_001327"
     CDS             6401..7615
                     /gene="gltS"
                     /locus_tag="WGO49_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ0306432.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            7714..8730
                     /locus_tag="WGO49_001328"
     CDS             7714..8730
                     /locus_tag="WGO49_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0306433.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            8741..8938
                     /locus_tag="WGO49_001329"
     CDS             8741..8938
                     /locus_tag="WGO49_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306434.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            8956..9105
                     /locus_tag="WGO49_001330"
     CDS             8956..9105
                     /locus_tag="WGO49_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306435.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFLCVSYADVPTHRTIQKAS
                     PYVPS"
BASE COUNT         2361 a         2550 c         2136 g         2106 t
ORIGIN      
        1 tggcttgata ccggcacaca cgccagcctg ttggaagcag cctcgtttgt cgaaaccatt
       61 gaaaacattc aaaacctaca agtcgcctgt ttggaagaaa tcgcatggcg taatggctgg
      121 ttgagcagcg aagaaatcag aaacttggca aagccaatgt gtaaaaatga atatggccgc
      181 tatttattgc gcttggttgg caaagattaa gaggttgctt ttaccagttt tcaggccgtc
      241 tgaaacgctt tttaggaaaa catgatgaat atcatcgata cccgcattcc cgatgtcaaa
      301 ttgctcgaac ccaaagtctt cggtgacgag cgtggttttt ttatggaaac cttccgtgat
      361 gaatggttcc gccaaaatgt agccgagcgc actttcgtgc aggaaaacca cagcaaatcc
      421 acccaaggcg tattacgcgg tttacattat caaaccgaaa atacacaagg caaactggtg
      481 cgcgtggtat caggcgaagt ctttgatgtt gccgtggata tgcgtcgtga tagtcctact
      541 ttcggccact gggtcggtga ggttttatcg gcagacaaca aacgccaatt atgggtgccg
      601 gaagggtttg cccatggatt ttatgtgttg agcgatgaag cggaattcgt ctacaaatgc
      661 accgactatt acaacccgaa tgcggaacat tccctaattt ggaatgatgc cacagtggga
      721 atcgactggc ctttattggg agaaccaagc ctatcggcca aggatttggc aggcaagatg
      781 cttgctgaag cggccacatt ttaattttca cttttaggtc atcgtgggta atttgtagca
      841 agatgaccta atttttacca gctagcctac taactaaatt tctactatat ttaaatagtc
      901 agaaaattta cacaaaatct gttaataaga tattatattg tcagtaatat tttttcctat
      961 ttattgtgat tcctgcctac attcctcttc gctctttcgg tatacgtaaa attccgaata
     1021 ttacgcaatt tctaccggaa tattcactta acaaaaccgt attcaacgcc caaaccatca
     1081 ttggctgggg cttacgcccg accacccacc aagcacgtgc ttttgcacaa tcacatggct
     1141 tgccgtttgt agccttggaa gacggttttt tacgctcact gggcttaggc gtggccggtt
     1201 atccgccctt cgcactggtt tacgacgata tcggcatcta ttacgacaca acccgcccat
     1261 cgcgtttgga gcagttgatt ttagccgcaa acatgccgtc tgaaatgctt aaccaagcgc
     1321 agcaggccat tgatttgatt ttgacgcacc gtttgtctaa atataaccat gctctggatt
     1381 tttcagacgg cctcgtacaa gacagcaaaa acattgtttt ggtaattgat caaactttcg
     1441 gcgatatggc gattcaatat ggcggcgcag atgaagccac attccgccaa atgtttcaga
     1501 cggccttagc cgaaaatcct gatgcggaaa tctgggtcaa aacccatccc gatgtattga
     1561 gcggcaagaa acagggttac cttactgatt tggcacagca gccgcgtgtg cgtttattgg
     1621 ctgaagacat taatccgatt tcgctgctgc aagcggtgga caaagtgtat tgcgtgacct
     1681 cgcaaatggg ttttgaagcc ttgttgtgcg gcaagccttt ggtaacgttt ggtttgccgt
     1741 ggtatgccgg ctggggtgta agcgacgacc gccatcctga aatcaaccgc cttgttcaaa
     1801 cccaacgccg cgccacccgc aacttgctgc agctcttcgc cgcagcctat ctgcaataca
     1861 gccgctacct caaccccaat accggcgaag caggcagcct ctttgatgtc atcgactatc
     1921 tggcgacggt caaacgtaaa aacgacaaat tgcgtggcga gttatattgc gtcggtatgt
     1981 ctttgtggaa acgcgcggtt gccaaaccgt tctttaacgt accctcttgc cgtctgaaat
     2041 ttatctcttc cacccaaaaa ctggcaaggg tcaaactgtc cgacgatgca cgcatcctgg
     2101 cttggggcaa cggcaaagag gccatcgtcc gctttgccga acaacaccac atccccctgc
     2161 tgcgcatgga agacggcttt atccgctcgg tcggactcgg ctccaactta gtgccgccgc
     2221 tgtcgctcgt taccgacgat atgggtattt acttcaatgc cgaagcccca tcccgccttg
     2281 aacacatcct gcaaaaccaa aacttcgacg atcaagactt tcagacggcc ttgaagttgc
     2341 aaaaaatgct gaccgaaaac catatcagca aatacaacgt cggcaactca gactttaccg
     2401 ccccatcaac cgacaaaacc gtgatcctcg ttcccggtca ggttgaggat gatgcgtcta
     2461 tccgctatgg ttcgccccaa atctatcaca atctggattt gctccgtacc gtacgcgaac
     2521 gaaaccccaa tgcctatatc atctacaaac cgcatcccga tgtagtcagc ggtaaccgca
     2581 tcggccatat ttcccctgaa gatgctgcac gatatgccga ccaaaccgcc gaacaagccg
     2641 acatcctgac ctgtctccaa tacgcagacg aaatacatac catgacttcg ctgaccggtt
     2701 ttgaagcctt gttgcgcggc aaaaaagtca gctgctacgg cctgcctttt tacgcaggct
     2761 gggggcttac ccaagatctg ctccccatcc cgcgccgtag ccgcagactt gagctttggc
     2821 agctgattgc cggcacgctc atccactatc ccgactacat ccaccccgaa acccatcagg
     2881 ccataaatgc ggaaaccgca gcccaaatcc tgatacgaca aaaaaatatg caaaaaaaca
     2941 acaacggatt acatcgcggg tgctttgcca aaaaattagg taaaatcaaa caactatatc
     3001 gatctttcaa ataaatacca tcaaagttaa cgatgcgtca taaacttgcc tctattgcgg
     3061 catcattgcc tttgcatcgt taattctctt ggcgtatgct tgaaagttca acctaaaact
     3121 attacataaa aaacaaaacc acattgcaac atgaaacaga ccgtcctcaa aaataacctg
     3181 caaaacctgc ttgaaagcgc agaaaatatc ctgctgcttc aaggccctgt cggcgatttt
     3241 tttctgcgcc ttgccgactg gctgactgca aacggcaaaa ccgtacataa attcaacttt
     3301 aatgcaggcg acgactattt ttatccgccc actcaagcgc ataccgttgt ttttaacgac
     3361 aactacgatg cctttcctga gtttttgcaa gaatacatca ctcaacatca catccaggcc
     3421 gttgtctgct ttggcgacac acgcccttat cacgtcattg caaaacgcat tgcaaacgaa
     3481 aaccaagcca gtttctgggc gtttgaagaa ggctatttcc gcccctacta catcacctta
     3541 gaaaaagacg gcgtcaacgc attttccccg ttgccgcgcc gtgccgactt ttttcttgaa
     3601 caattcccta agcttgccca gcaagaatat aaagcgccaa cgccggtaca cggcggtttt
     3661 acgcccatgg caaaaaacgc tatccgttac tatatcgagt tgttccgcaa tccacgcaaa
     3721 taccccgact acatccacca ccgcgcaccc aatgccggcc attacctcaa accgtggtcg
     3781 ctctccatcc tcaagcgttt gaactactat attgaagaca tccaaatcgc caaacgtgtg
     3841 gaagcaggca aatacggcaa gttttttatt gttcccttac aggtattcaa cgacagccaa
     3901 gtccgtatcc attgcgactt tcccagcgtc cgcagcttcc tgctccatgt tttgagttca
     3961 tttgccgagc acgcgcctgc cgataccaac atcatcatca agcatcatcc gatggaccgc
     4021 ggttttatcg actactggcg cgacattaaa cgctttatca aagaacaccc cgaactcaaa
     4081 ggccgcgtga tttatgtcca tgatgtcccc ctgcccgttt tcctgcgcca cggtctcggc
     4141 atggtcacca tcaacagcac cagcggcctg tccggactga ttcacaatat gccagttaag
     4201 gttctcggcc gtgcctatta tgatattccc ggcattactg accaaaatac cttggcagaa
     4261 ttttggaatc atccgacacc gcctgacaaa gagctgttcc atgcctaccg aatgtaccac
     4321 ctcaacgtga cccaaattaa cggcaacttc tacagtcagg tgtttttccc caacaaaaaa
     4381 acctccaact cttccacacc agtaatctga cttagcgaag gaagttcagg ccgtctgaaa
     4441 acatttcaga cggcctgaaa caatcaatac cttagctact gccatgtaaa taaaacacaa
     4501 aaatctgcat ttatcattaa caataaatta caaaaacagt ataatgaccg agctgccatg
     4561 agcgcatacc gactcaacct gagcccttta taacacacaa aatatggatg tatccccagg
     4621 caaaacaata taacaagcca aacatcctaa agataagccg gcaaggaaat acactctgca
     4681 aaacgatgcc gagcaatatt tttacaaagc cctcaaccgg tatcgccgcc catatgccgc
     4741 agcatccgtc ttccacttta tatccgcccg caaaccatga ccgccgctcc tgatatccta
     4801 taccgtcaag ccgccgccct tttggaacaa tccaataccg cccaagccct gcccctgctg
     4861 caacaggccg cagagcaggg atatacggaa gctgcttttg tattgggcga ccatctgctg
     4921 caaaacggtc atccggagca ggcacttcca tggttggaag ccgccgcggc ccaacgccat
     4981 cccaaggcac tctttaccct gctgcaacaa cgcgaacaca acggcacccc gaccggacag
     5041 cttcttaacg aatacgcctg gctgagtgag caaggtcacc tcgaagccca attaattctc
     5101 atgcgttacc acacgcagcg caacgatcca caatcgctct actgggcgga gcttgccgct
     5161 gcccaatatg ccgcacctgc gtattaccat ttggcacgcc atcatcaacg ccaaggcgac
     5221 gttgcaacag ctatcgaaca atacgaaaaa gcggcagcgc tcggcgtgac tgcagcctgc
     5281 tggcaactcg gtcaaatcta cttctacggc acaggtgtca gccccaacca cgcacaagcc
     5341 gaacactatc tcgaacaagc cgcgcaagcc ggccacatcg ccgcacaaac cctgctggcc
     5401 gaccttcttg ctgcccaacg caaacccgaa gccttagaat ggtatcgtcg tgccgccgac
     5461 aaagagcaag cggaagcaca ggccaagctg gctcaatacg ccctgaccgg cgaactttcc
     5521 gaacgcgatc cgtttcaagc ggcacgatat gccaaagccg ccgctgagaa agaccatccc
     5581 gaagccctga aaatcatggg cgacctctac cgctacggcc tcggcatcaa agctgacaac
     5641 catatcgcgc acgattacta ccaccgtgct gccgcgctgg gttctgccgc cgcagcacaa
     5701 aaactcatca gcgacgccgc gctgtaccat ccgcaacaat acgaacaaat caaaactgcc
     5761 gccctgcaac agcaacaaac cgaaaccatc taccgtttgg cggaaacaca agcccatgcc
     5821 atcggtcgtc ccgccgacta taatgccgca cgcaaaaatt acatggaagc tgccgggttc
     5881 caccataaaa acgcggcggc tgccttaggc cgcatctacc attacggcct cggtacggcg
     5941 caagatcctc gggcggctgc acactggtac gccattgctg ccgaacaaaa ccacccttcc
     6001 gcccaatacc acctcgcctg tttttactat cacgggcaag gtgtcggctg tcatgttccg
     6061 accgcctgct actggctgca ggccgccatc ggcaacggcc acacttcggc cgaatcatta
     6121 atatccctat tagaacaatg gcgacgcgaa gcacaccatg ccatcggaca aaaggccgtc
     6181 tgaaaagatt tacactcgca ttttttgaca atctttaact attcccctaa tatttgccag
     6241 ttatttttca cggacacgcc attgttttca tttctttctg aaaacacctt gtccgcgcat
     6301 caataccatg acactcggcg gataacgcca agcgttgaaa cacactacat ccggaacaaa
     6361 aacggatgct cggaaaaata tttctaggag gtgaaacaac atggaatggg aattcaacag
     6421 ttattacaca ctgattgccg ccacgctcgt gttgctggtt ggtaaatttc tggttcaaaa
     6481 aatcaaattc ttacgagact tcaatattcc cgagccggtc gctggcggtt tggttgccgc
     6541 gattatcctg tttgcgctgc acgaggcgta cggcgtgagc ttcaaatttg agaaaccgct
     6601 gcaaaatgcg tttatgctga tttttttcac gtccatcggc ttgagcgcgg atttttcccg
     6661 tttgaaggcg ggcggtttgc cgctggtggt ttttaccgcg attgtgggcg gatttatctt
     6721 ggtgcaaaac tttgtcgggg tcggactggc tacggctttg ggtttggacc cgctcatcgg
     6781 tctgattacc ggttcggtgt cgctgacggg cggacacggt acgtcaggtg cgtggggacc
     6841 taattttgaa acgcaatatg gcttggtcgg cgcaaccggt ttgggtattg catcggctac
     6901 tttcgggctg gtgttcggcg gcctgatcgg cgggccggtt gcgcgccgcc tgatcaacaa
     6961 aatgggccgc aaaccggttg aaaacaaaaa acaggatcag gacgacaacg cggacgacgt
     7021 gttcgagcag gcaaaacgca cccgcctgat tacggcggaa tctgccgttg aaacgcttgc
     7081 catgtttgcc gcgtgtttgg cgtttgccga gattatggac ggcttcgaca aagaatacct
     7141 gttcgacctg cccaaattcg tgtggtgtct gtttggcggc gtggtcatcc gcaacatcct
     7201 cactgccgca ttcaaggtca atatgttcga ccgcgccatc gatgtgttcg gcaatgcttc
     7261 gctttcgctt ttcttggcaa tggcgttgct gaatttgaaa ctgtgggagc tgaccggttt
     7321 ggcggggcct gtaaccgtga ttcttgccgt acaaaccgtg gtgatggttt tgtacgcgac
     7381 ttttgttacc tatgtcttta tggggcgcga ttatgatgcg gcagtattgg ctgccggcca
     7441 ttgcggtttc ggcttgggtg caacgccgac ggcggtggca aatatgcagt ccgtcacgca
     7501 tactttcggc gcgtcgcata aggcgttttt gattgtgcct atggtcggcg cgttcttcgt
     7561 cgatttgatt aatgccgcga ttctcaccgg ttttgtgaat ttctttaaag gctgattttc
     7621 cgcctttccg ccaaagcacc tgcaaggttt accgcctgca ggtgcttttg ctatgatagc
     7681 cgctatcggt ctgcaccgtt tggaaggaac atcatgtatc ggaaactcat tgcgctgccg
     7741 tttgccctgc tgcttgccgc ttgcggcagg gaagaaccgc ccaaggcatt ggaatgcgcc
     7801 aaccccgccg tgttgcaagg catacgcggc aatattcagg aaacgctcac gcaggaagcg
     7861 cgttctttcg cgcgcgaaga cggcaggcag tttgtcgatg ccgacaaaat tatcgccgcc
     7921 gcctacggtt tggcgttttc tttggaacac gcttcggaaa cgcaggaagg cgggcgcacg
     7981 ttctgtatcg ccgatttgaa cattaccgtg ccgtctgaaa cgcttgccga tgccaaggca
     8041 aacagccccc tgctgtacgg ggaaaccgct ttgtcggata ttgtgcggca gaagacgggc
     8101 ggcaatgtcg agtttaaaga cggcgtattg acggcagccg tccgcttcct gcccgtcaaa
     8161 gacggtcaga cggcatttgt cgacaacacg gtcggtatgg cggcgcaaac gctgtctgcc
     8221 gcgctgctgc cttacggcgt gaagagcatc gtgatgatag acggcaaggc ggtgaaaaaa
     8281 gaagacgcgg tcaggatttt gagcggaaaa gcccgtgaag aagaaccgtc caaacccacg
     8341 cccgaagaca ttttggaaca caatgccgcc ggcggcgatg cgggcgtacc ccaagccgca
     8401 gaaggcgcgc ccgaaccgga aatcctgcat cctgacgacg gcgagcgtgc cgataccgtt
     8461 accgtatcac ggggcgaagt ggaagaggcg cgcgtacaaa accagcgtgc ggaatccgaa
     8521 attaccaaac tttggggagg actcgatacc gacgtgcaaa aagagttggt cggcgaacaa
     8581 cgcaagtggg cgcaggaaaa aatcagcaac tgccgacaag ccgccgcgca ggcagaccgg
     8641 caggaatacg ccgaatacct caagctgcaa tgcgacacgc ggatgacgcg cgaacggata
     8701 cagtatcttc gcggctattc catcgattag gggcaaaccg atgactaccg tccccaaaag
     8761 caggattccc gtcaaaccgc tgcccgaaaa aaccacagac gaagccaaag tcgaaaaatg
     8821 gcggcagctc ggtgcggaac acggtttgtc gggcgaatgg gcagttgccg ccagattggg
     8881 cgagaacggt tttaccgaag aacagatgga aaatatcgcc aacctgttcg gcagataaag
     8941 ggaaaattga cggaaatgcc gtctgaaacc ctgttatcgg tttcagacgg cattttgact
     9001 aatacggtac gcaggcgcaa aacagccggc tttttatgtg tttcctatgc tgatgttcca
     9061 acacacagga cgatacaaaa agcgtcgccc tatgtgccgt cctgattcgg aaagggttac
     9121 gctcctccca aatatagtgg attaacaaaa acc
//
LOCUS       DAWFAD010000078         8922 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWFAD010000078 DAWFAD010000000
VERSION     DAWFAD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.078"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            188..622
                     /locus_tag="WGO49_001331"
     CDS             188..622
                     /locus_tag="WGO49_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0306436.1"
                     /translation="MNFALSVITFTLASFLPVPPAEAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(671..2014)
                     /locus_tag="WGO49_001332"
     CDS             complement(671..2014)
                     /locus_tag="WGO49_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ0306437.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLVSDGIPLAGAWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(2019..2672)
                     /locus_tag="WGO49_001333"
     CDS             complement(2019..2672)
                     /locus_tag="WGO49_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ0306438.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIVAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2823..3113
                     /gene="gatC"
                     /locus_tag="WGO49_001334"
     CDS             2823..3113
                     /gene="gatC"
                     /locus_tag="WGO49_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ0306439.1"
                     /translation="MALTLADVDKIARLSRLHLTAAEKEKSLQELNDIFAMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRNRLYIVPQVIEE"
     gene            3176..4621
                     /gene="gatA"
                     /locus_tag="WGO49_001335"
     CDS             3176..4621
                     /gene="gatA"
                     /locus_tag="WGO49_001335"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ0306440.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAITGFDPKDSTSLEREKEDYTRDLNQPLKGVKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4618..5550
                     /locus_tag="WGO49_001336"
     CDS             4618..5550
                     /locus_tag="WGO49_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HEZ0306441.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEARGISDGIQTEKPLRRTA"
     gene            5593..7023
                     /gene="gatB"
                     /locus_tag="WGO49_001337"
     CDS             5593..7023
                     /gene="gatB"
                     /locus_tag="WGO49_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ0306442.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPELR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGSKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ASGQGKPTANWMNGELAAALNKAGLELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPESTIAEIIEKHGLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7115..8125
                     /locus_tag="WGO49_001338"
     CDS             7115..8125
                     /locus_tag="WGO49_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ0306443.1"
                     /translation="MNHTVTLPDQTTFAAGDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQIGGHSEQALSEAEKAQGKILMCCTTAQSDISLNIPGYKADALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANSPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSGRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8254..8886)
                     /gene="pdxH"
                     /locus_tag="WGO49_001339"
     CDS             complement(8254..8886)
                     /gene="pdxH"
                     /locus_tag="WGO49_001339"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ0306444.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
BASE COUNT         2278 a         2744 c         2171 g         1729 t
ORIGIN      
        1 tctgcggctt cgtcgccttg tcctgatttt tgttaatcca ctatattttt gggaatctgt
       61 tttaccccaa tatataaagc accatattaa ggcggagttt cttccccact ttgaaccgaa
      121 cccggaaaag acaccgccca agccaatcct gatgctgccc cggcagccaa ccatcaagga
      181 aaccctaatg aactttgctt tatccgtcat cacatttacc ctcgcctctt tcctgcccgt
      241 cccgcccgcc gaagccgccg tctttacttg gaaggacggc ggcggcaaca gctattcgga
      301 tgtgccgaaa cagcttcatc ccgaccagag ccaaatcctc aacctgcgga cgcgccaaac
      361 caaaccggcg gtcaaacccg cccaagccga cgcagggaag cgcacagacg gcgcggcaca
      421 ggaaaacaat cccgacactg ccgagaaaaa ccggcagctt gaggaagaaa agaaaagaat
      481 tgccgaaacc gaacggcaga acaaagaaga aaactgccgg atttcaaaaa tgaacctgaa
      541 agcggtggga aattcaaatg caaaaaacaa ggatgatttg attcggaaat acaataacgc
      601 cgtaaacaaa tactgccgtt aatcggctct agcgcaaacc ccatgccgtc tgaaacggca
      661 cggggtttgt ttatttctgc cagtaggttt tgacgttgac gaactcgtac agcccgaatt
      721 cggacaattc gcgcccgtaa ccggaatctt tgactccgcc gaaaggcagg cgcaaatcgc
      781 tgctggtatg gcggttgata aacaccgatc ccgcctgtat tttttcggca aaccgccaag
      841 cgcgttcggt atcggcggta taaatgcagg cacctagccc gaacggggaa tcattggcaa
      901 gggcgatggc atgttcttcg ttttcggcgc gcaaaatcag ggcggccggc ccgaatactt
      961 cttctctcca gacgcggcag gcaggattta ccctgtctaa aaccgtcgcg ggataaaacc
     1021 agcctcgccc ttgtgggatt tttccgccgg tcaggcatac cgcgccgttt gaaacggcat
     1081 cttcaacctg cccgtgaacc ctgtcccgca aatcttcgcg gtgcagcggt gcaagcgtag
     1141 tatcgggatg tttggggtcg cccattttca atttggcgca ttcggcaaga aacagcgtga
     1201 taaaacgatc ggctgcggct tcggttacga tgatgcgttt ggcggcgttg cacgattgcc
     1261 ccgcatcgcg gaaacgggaa taacaggctt ctgcggcggc gcgttccaaa tcggcatcgg
     1321 gcatcacgat aaaggcgttg ctgccgccga gttccaagac ggttttcttg aggtttgcgc
     1381 ccgcatgtgc cgcaaggatg cgccccgtat gcgtcgagcc ggtaaacgcc atcgcatcgg
     1441 tatcttcaac cgccttgagc gtgcccgcct catccaacca cgcgcctgcc aaaggaatgc
     1501 cgtctgaaac caaatcaaac aacgcctgac tgacgcgtgc cacgctgggc gcgggtttga
     1561 cggcgcacgc gttgcccgcg cacatagcgg gaacggcgaa acgcaatacc tgccagacgg
     1621 gatagttcca aggcatgacg gcaaacacca cgcccaaagg ctcaaacctt acctgactca
     1681 aactcgcctg cgtcgcgatg gttttgtggg cgagcagttc gggagcgagg cgggcgtaat
     1741 agcgtatcag ttcgatagac ttgccgattt ccgcacggca ttcgtgcagg cagcgtccga
     1801 cttcttcgca caccatttcc gcaaaacgct ctttctccgc ctccaaacgg tcggcaaatt
     1861 tttgcagacg cgcggcacgt tcggttacgc ccagttgcgc gaacgccccg ccgcgcattt
     1921 tcaaatccgc cagccgccgt tcaaactccg cataatcttg agctggacgg cggtaaagcg
     1981 tttcgcccgt aaatacattg acactgtgaa acatcgaatc aacctgccag ttgcgggaat
     2041 atcgttttca gtcccgacac aataatctcc accgataccg ccgccagcat catacccatg
     2101 atacggttta aaatcgtcag ccccgtcgcg cccagcaggc ggctgacctt ccctgcaaca
     2161 attaaaatgg cataacaaat cgcactgacc accaaaccgg ccacgataat cagcgcgatg
     2221 tcgctgtacg ttttggctgc cgaagcgtaa ataatcacgg tcgaaatacc gcccgggccg
     2281 atggtgatcg gtatggcgat gggcacgacg gcaatcgctc cggcattgcg ggcggggcgc
     2341 gcctgccccg tttccggctg cgcgccgaga ttctgcttgg cgggattgtc gttgccgttc
     2401 atcatcgaaa tggcgatcag cagcaccaaa atcccgccgc cgacctgaaa cgaaccgacg
     2461 ctgatgccca aaaccttcag cagcgtaccg ccgatcagcg caaataccgc aatcacggca
     2521 aacacggcaa cggcggccgt ccgcgcgacc ttcctgcgct ccttcgtgct gtgcccgttg
     2581 gtcaggtcaa ggtaaagcga caacgcgcta aacggattaa tcagcaccaa aaaagccaca
     2641 atcagcttgc cgatttccat gcccaatccc attatttccc cctccttcaa acccgtgcgg
     2701 caggcatccg atgctgcaaa ttgccgccgc aacggatttt tccgttataa ttaaaaattc
     2761 aagcaatacg ccccatcata cccgaacgac ggtatcttta ccatcagaca aggatgcttt
     2821 tcatggcact gacacttgcc gacgtagaca aaatcgcccg actctcccga ctgcacctga
     2881 ctgcggcaga aaaagaaaaa tcgcttcaag aattaaacga cattttcgct atggtcgaac
     2941 agatgcaaac catcaacaca gacggcatcg aaccgatggc gcatccgcac gaggccgccc
     3001 tgcgcctgcg cgaagacgaa gtaaccgaaa ccgaccgcgc cgccgaatat caggcggttg
     3061 ctccggaagt acgcaaccgt ctgtatatcg taccgcaagt tatcgaagaa taatccgaat
     3121 atgcttcaga cggcatcagc aataccgccc gaagcccttt aaggatggaa gatttatgac
     3181 ccaatacaca ttgaaacagg caagcgtcct gttgcagtcc aaacagattt ccgccgtcga
     3241 actggcaagc gcataccttg ccgccatcgc cgaaaaaaat cccgccctca acggctacat
     3301 caccatcgac caagataaaa cccttgcaga agcccgtgcc gccgacgaac gtatcgcgca
     3361 gggcaacgcc tccgcgctta ccggcgtacc cgtcgcctac aaggatattt tctgccaaac
     3421 cggctggcgc agcgcgtgcg cttccaaaat gctcgacaac ttcatctccc cttacaccgc
     3481 caccgtcgtc caaaacctgc tcgacgaagg catggtaacg ctcggccgca ccaatatgga
     3541 tgagttcgct atgggttcga ccaatgaaaa ctcattctac ggcgcggcca aaaatccgtg
     3601 gaatccggaa cacgtccccg gcggttcgtc aggcggttcc gccgccgtcg ttgccgcgcg
     3661 ccttgcccct gccgcgctcg gttcggatac cggcggctct atccgccagc ctgcatcgca
     3721 ctgcggcatt accggcatca aacctaccta cggcacggtt tcccgcttcg gtatggtcgc
     3781 ctacgcctcc agcttcgacc aggccggccc gatggcgcaa actgccgaag actgcgcgat
     3841 tctgttgaat gcaataacag gtttcgaccc caaagactcc accagtctcg aacgtgaaaa
     3901 agaagactac acccgcgatt tgaaccaacc gctcaaaggc gtgaaaatcg gtctgcccaa
     3961 agaatatttc ggcgaaggca acagcgccga tgttctgacg gcattgcaaa acaccattga
     4021 tttgctgaaa gcccaaggcg cggaattgat tgaagtttcc ctgccgcaaa ccaagctgtc
     4081 catccccgcc tactacgtcc tcgcctccgc agaagccggc accaaccttt cacgttacga
     4141 cggcgtacgt tacggacacc gtgccgccca attcgccgat ttggaagaaa tgtacggcaa
     4201 aacccgcgcc gaaggtttcg gcagcgaagt caaacgccgc atcatgatcg gcacttatgt
     4261 actgtcgcac ggctactacg atgcctacta tctcaaagcc caaaaactgc gccgcctcgt
     4321 tgccgatgat tttcagacgg catttgcacg gtgcgacctc atcctcgcgc cgaccgcacc
     4381 cactgcagcc ccaaaaatag gagcggatgc ttcgccggtt gaaacctact tgagcgatat
     4441 ctacaccatc gccgtcaacc tcgccggact gcccgcattg accctgcccg caggcttcag
     4501 cggcggcgga ctgcccgtcg gcgttcagct tgtcggcaac tacttcgccg aagccaaaat
     4561 cctcggtgcg gcgcatcaaa tccaactcaa cagcgattgg cacggcaaac gacccgaatg
     4621 aagcagaacc gcacctttac cttccccgat tttcgcaccg tttacagcta tgcgccttta
     4681 tatcggctgc aacatttaaa atacacattg cgaaaatttt tcggaaaaaa agaaatttac
     4741 gccttcgagc agtttgtcaa cgcctcccct atccgtcagg ggctgttcct ccactgcccg
     4801 caaaatgcct atccgctgct gcgcgaattt gttgacaggc gttttaactg caaacgccgt
     4861 ttagatgcga tgacggcaga ttttctcatg gcggaaaaac tgttcggcac agacatcctg
     4921 caccaaatgg aagactaccg cttccatttg gtcttggcgc acctttcaga cggcatcagc
     4981 ttgtggctca accgcaacga caactgcgtc gaagaaggcg cgtggtcttt atctttgcgc
     5041 gacgaagcag gcaaccggct gtatatggcg actttcgcct ttgtcggcac acacctgctg
     5101 acagcctccg tacaagggcc tgcgggtgaa gaagccaaag acactgtccg ccgcataacc
     5161 aaacaacttc acggcttgcg tccccaacaa ctgatggtaa ccgcactgca atatttcgcc
     5221 gccgtactcg gcttggacgg cgcaatgggc attgcacaaa aacatcaggt caaactgcgc
     5281 tggaaactta aaaagcgcgt caaaatgaat tacgacgcat tctggcagga atacggcgca
     5341 agtttggaac gggacggcta ctggcatctc ccccaaaccc ccgcccgcaa agaccttacc
     5401 gacatcgaaa gcaaaaaacg ctcgatgtac cgcaagcgtt atgaaatgct ggacaacatg
     5461 gttgcagaga tgaaggacgg tctgaaaaca gaagcacgcg gcatttcaga cggcatccaa
     5521 acggaaaaac cgctccgccg gacagcctga cgcgaagact atcgaattga tattttagag
     5581 aaagaagctc ttatgacctg ggaaaccgta atcggcttgg aaatccacgt ccaattgaac
     5641 accaaatcca aaatcttcag cggcgcatcg accgcattcg gcgcagaacc caacgcgcac
     5701 gccagcgtag tggaatgcgc gctgccgggc gtactgccgg taatgaaccg agaagtcgtt
     5761 gaaaaagcca tcaaattagg cttggctttg gatgcgaaaa tcaaccggaa aaacgtgttc
     5821 gaccgtaaaa actacttcta tcccgactta ccaaaaggct atcaaatcag ccagttggat
     5881 ttgccgattg tcgaacacgg caaattggaa atcgtagtcg gcgacgatgt gaaaaccatc
     5941 aacgtaaccc gcgcccatat ggaagaagac gcgggcaagt ccgtgcatga aggcttgaac
     6001 ggcgcgaccg gtatcgacct gaatcgcgcc ggcacgcctt tattggaagt cgtatccgaa
     6061 ccggaattgc gctccgccgc cgaagccgtt gcctacgcca aagccttgca cagcttggta
     6121 acctggctgg acatttgcga cggcaatatg gcggaaggtt cgttccgcgt cgatgccaac
     6181 gtatccgttc gcccgaaagg tcaagcggaa ttcggcacgc gccgcgagat taaaaacctc
     6241 aattccttcc gtttcttgga gcaggcgatt aattacgaag cggaagcgca aatcgagatt
     6301 ttggaagacg gcagcaaagt gcagcaggcg acgatgctgt tcgacccaga aaaaggcgaa
     6361 acccgcgtaa tgcgcctgaa agaagacgcg caagactacc gctacttccc cgacccggat
     6421 ttgttgcccg tcatcatttc agacgctcaa atgcaaaaag ccaaagcaga aatgcccgag
     6481 ctgccgaaag aaatggcggc acgtttcgtg gcagattacg gcgtgtccga atacgacgcg
     6541 cgcctgctca ccgcaagccg cgcacaggcc gcctattttg aagaagccgc caaagcaagc
     6601 gggcaaggca agccgactgc caactggatg aacggcgaac tcgccgccgc gctgaacaaa
     6661 gccggcctgg aacttgccga cagcccgatt accgccccgc gcctcgccgc gctggtgggc
     6721 aaaatcgccg acggcacatt aagcagcaag ttagcgaaaa aagcctttga agccatgtgg
     6781 gcagaacccg aatccaccat tgccgaaatc attgaaaaac acggcttgca gcaaatgacc
     6841 gataccggcg cagttgaagc gatggtggac gaagtgctgg caaacaacgc caaagccgtg
     6901 gaacagttta aatccggcaa cgaaaaagcc ctgaatgcga ttgtgggaca agtgatgaaa
     6961 gccagcaaag gcaaagccaa ccccgcgcaa gttcaagaac tgattaaagc caaacttgcc
     7021 tgataagcaa gatgccgtct gaaacccaga tccgacgttc agacggcgtt ttcacagtct
     7081 gaccacatta aaacagcata aaaaacaata ttccatgaac cacaccgtta ccctgcccga
     7141 ccaaaccacc tttgccgccg gcgacggtga aaccgttttg gccgctgccg cccgtcaaaa
     7201 cctcaatctg ccccattcct gcaaaagcgg tgtctgcggg caatgcaaag ccgaactggt
     7261 cagcggcgat attcaaatag gcggacactc ggaacaggct ttatccgaag cagaaaaagc
     7321 gcaaggcaag attttgatgt gctgcaccac tgcgcaaagc gacatcagcc tcaacatccc
     7381 cggctacaaa gccgatgccc tacccgtccg caccctgccc gcacgcatcg aaagtatggt
     7441 tttcaaacac gatgtcgccc tcttgaaact tgccctgccc aaagcccctc cgtttgcctt
     7501 ctatgctggg caatacatcg atttactgct gccgggcaac gtcagccgca gctactccat
     7561 cgccaattcg cccgaccaag aaggcatttt ggaactacac atccgcaggc gcgaaaacgg
     7621 tgtctgctcg gaaatgattt tcggcagcga acccaaagtc aaagaaaaag gcatcgtccg
     7681 cgttaaaggc ccgctcggtt cgtttaccct gcaggaagac agcggcaaac ccgtcatcct
     7741 gctggcaacc ggcacaggct acgcccccat ccgcagcatc ctgctcgacc ttatccgcca
     7801 agacagcggc cgcgccgtcc atttctactg gggcgcgcgt catcaagatg atttgtatgc
     7861 cctcgaagaa gcacaagggt tggcatgccg tctgaaaaac gcctgcttca cccccgtatt
     7921 gtcccgcccc ggagagggct ggcagggaag aaaagggcac gtacaagaca tcgcggcaca
     7981 agaccacccc gacctgtcgg aatacgaagt atttgcctgc ggctctccgg ccatgaccga
     8041 acaagcaaaa aatctgtttg tgcaacagca taagctgccg gaaaacttgt ttttctccga
     8101 cgcattcacg ccgtccgcat cataattccc cggtataaaa aagattcagg ctttccgttc
     8161 ggaacacaaa aaacttcccg tccgtatttt ccccataaaa aatgccgtct gaagcccgat
     8221 tccggttttc agacggcata tgttttttcc tgttcaaggc gacagccgct cgcgtatcca
     8281 gccaccatcc agcaaacggt attggatgcg gtcgtgcagc ctgctcggcc tgccctgcca
     8341 aaactcaatc aaatcgggaa tcacaatata gccgccccaa tgcggcggac gcggcacgtg
     8401 caaaggatgt ttgagtccga ccgccgccgc ctttgccacc aataccgcct tgttcggaat
     8461 cacctcgctc tgcgcacttg cccacgcacc caaacggctc tgatacgggc ggctctcaaa
     8521 atattcgtcc gacaacttct ccgccagcct ttcaacacgc ccttccacgc gcacctgacg
     8581 ctccagctcc ggccaaaaaa acgtcatcgc cgcaaatgga tgagcatcca gcgaacgccc
     8641 cttgcggctg tgataattcg taaagaaaac aaaaccttca gaattaactt ccttcagcag
     8701 caccatacgg ctgttaggcc tgccgcgtcc gtcgaccgcc gccacattga ccgccgtcgg
     8761 ctcgttgacc tgtgcgcgta ccgcctcgtc caaccaccgc tcgaactgct cgatcggatt
     8821 gtcggcgcaa tcgccttccg acaattcccg tttgctgtaa tcttcccgaa tattgtgcaa
     8881 atccatttac cgctcctctt aatagtggat taacaaaaac ca
//
LOCUS       DAWFAD010000079         8830 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWFAD010000079 DAWFAD010000000
VERSION     DAWFAD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.079"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            179..1354
                     /locus_tag="WGO49_001340"
     CDS             179..1354
                     /locus_tag="WGO49_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ0306445.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIKRESKLQTNAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(1565..2113)
                     /locus_tag="WGO49_001341"
     CDS             complement(1565..2113)
                     /locus_tag="WGO49_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ0306446.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WGO49_001342"
     CDS             complement(2116..3612)
                     /gene="ccoG"
                     /locus_tag="WGO49_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ0306447.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            4140..6119
                     /gene="tkt"
                     /locus_tag="WGO49_001343"
     CDS             4140..6119
                     /gene="tkt"
                     /locus_tag="WGO49_001343"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ0306448.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEKGAKLEAEWDKLFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DGYVQTALKEVCAKAETVATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIINGLVLHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDTAYQAAVLPEGLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKEFGFTVDNVVDTVKSVL"
     gene            6267..7655
                     /gene="fumC"
                     /locus_tag="WGO49_001344"
     CDS             6267..7655
                     /gene="fumC"
                     /locus_tag="WGO49_001344"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ0306449.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(7720..8643)
                     /locus_tag="WGO49_001345"
     CDS             complement(7720..8643)
                     /locus_tag="WGO49_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0306450.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKWRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAKPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
BASE COUNT         2233 a         2538 c         2161 g         1898 t
ORIGIN      
        1 cactataata ttttctctcc cgattgaaac aggcgtaaca gaatatgccc gaagctccgg
       61 ctgctttctt gtttaccgcc gcgatattta gagtataata ccaaatttga gcaatagttc
      121 taaaacagtt agaaccattt ttcatgagcc tgactgattc gtacactcgg agaaactgat
      181 gcagaatatt tttgaccctt tggttattcg tggaaaatcc cttaccccca tcgtgcaagg
      241 cggtatgggg gtcggtgttt ccgcatcggg tttatccagc gcggtggcgc gtgaaaacgg
      301 tatcggaacg attgccagtg tggatttgcg ccaccttcac gaagacctac tcgccgaatc
      361 acaaatcaat ccgagtgaag agaaatatac atctttgaac tgtaccgcat tagacaggga
      421 aatccaaaaa gccaaaagcg cttcagaggg aaaaggactg attgcggtca acgtgatgaa
      481 ggcggtcaaa gaccacgccg cgtatgtccg ccaggcttgc gaatcagggg cggatgcggt
      541 tgtaatgggt gccggcctgc ctttagacct gccggaaatg accgagggct atcataaaga
      601 tgtcgcgctg ctgccgattc tgtccgaatc gcgcggtatt aatatcgtct tgaaacgttg
      661 gatgaaaaaa ggcatattgc ccgatgcgat tgtagtcgaa catcctgccc acgcggccgg
      721 acatttgggt gcatcaaccg ttgaaggcgt aaacgatgcc aagttcgact tcaaacgcgt
      781 gattgaggaa acgtttgaag ttttcaaaag tttagggctg gaaagcgaaa aaatcccgct
      841 tattcttgcg ggaggcatgg caaattttga aaaagtcaaa accgccctaa agaactgggg
      901 agcatccgcc gttcaaatcg gtacggcttt tgccgttacc gaagaaggag atgcacacct
      961 taacttcaaa aaaacgctcg ccggtgcgga aactgaaaaa gtagtcgaat ttatgtctgt
     1021 tgccggtttg ccggcgcgcg gtgtccgcac caaattccta gacagctaca tcaagcgtga
     1081 aagcaaactt cagacaaacg ccaaagccga cccgcgccgc tgtacccaag gtttaaactg
     1141 cctaaccagt tgcggtctgc gcgacgggct ttccaaagca ggacagttct gtattgatat
     1201 ccagcttgcc gccgcattcc gtggagaagt agataaaggc ctgttcttca gaggtaaaga
     1261 cccgctgccc ttcggcaatg ccatccgcac cgtccgcgag acgatacaat atctgctgac
     1321 ggggagcgaa cctgttgcaa cgctcggacg ctgacatcag gtaggatttg atttgcaaac
     1381 aaaatgccgt ctgaagggct ttcagacggc gtttttcagg ctgcgttccg gaatagtgtt
     1441 aaaaaataaa cgggatgaga tacatttatt ttcgtccgac aaatcaaacc atcgcgccga
     1501 atgatcaaat aatgcctgca cggcattaca tctggcaaag caatgcaatg aaaacacggc
     1561 ttttttattt gctttcagta ttattgaaaa gcttgtccat cggggtcaaa tcgaccgcat
     1621 tgccttggct ggtaatccat ttgttctcga cgcgccacac gcctgccgcg tcctccacgc
     1681 gcacatacca gtggttggtc ggcgaaaggg ttttgaacac cgcctcatat tccgccctgc
     1741 cgttctgcgc gctgccgacg ggcttgaggg cgacggtttg atcgtccgcc ttgcgggtcg
     1801 ggtgcatcag cagcaggttc aaaggctgtt tgccgtcaaa ctcgccgccg acaaacactt
     1861 ttgccgcatt catatcgggg gaaatgagaa cctgcacccc gatatgccgt ctgacggctt
     1921 cttcatcccg atgaagctgg atgtcgatat gtttgccgtc tttataataa tcgtccgtaa
     1981 ccaaatctgt cgcgtgctgc tgcgcgacaa aaaacatagc gacgctggcg atgacgacaa
     2041 aaatcggccc cgccatcaag atccacggcc agacgtgttt gtaccaaggt ttgattggag
     2101 tgttttgaga cacggttatt ctccgataaa ggttgcatct tcttccaaga cgaccggctt
     2161 gccgtcgggc gcgccgcttt cgcggtattg gaaggtaaat tcgatagggt ggctgccttt
     2221 gtccgcgtat tccggaatgg tggacacttg gacgggaagg gttaccgttt cgcgcggggc
     2281 aaccttgata ccgccttcgg gcagcccggt cagggcgatt tcgtcaaagc ctttgacact
     2341 tgcggtaatg agctgttctt tttcactttt gttgatgata cgcaggctgt atgcgttttc
     2401 cagccagcct ttggcgtttt cgcgcaccag tacgccacgg tctttcaaaa tatcgacctc
     2461 gaccattttg cgcgtggaca aaccggccag gaaggcaatg ataactaacg ccaacaccgc
     2521 gccgtaacct gccacgcgcg gtctgagcag ccgtttttta atgtcttttt cagaatattc
     2581 gtgttccagc gcgctttcgg tcgtataacg gattaatccg cgcggatagc ccattttgtc
     2641 cataatctca tcgcacgcgt cgatacaggc ggcgcagccg atacattggt attgcagacc
     2701 gttgcggatg tcgatgccga cggggcagac ttggacgcac atcgcacagt tgatgcagtc
     2761 gcccaaaccc gcctcttcct tattgaccgt tttcttgcgc gcgccgcgcg gttcgccgcg
     2821 ttccgcgtca taagaaacaa tcagcgtgtc cttgtcgaac atcgcgcttt ggaaacgtgc
     2881 atacggacac atatgcaggc atactttttc acgcataatg tgggcgaaga agaaggtcat
     2941 aaagccataa aacgctgcgg caaacatcgc gccgccacct gctgctccag tgaataaatc
     3001 gggaacgaac tggcggatag ggacaaacca gcctgcaaac gtgatgcccg tccacgcgca
     3061 gacaaggaaa atcagcaggt atttggtggc tttgatgcgg attttagtga aattccacgg
     3121 cgatttttcc agtttcagcc gtttgtttct atcgccttcg accaggttgt caatccacag
     3181 cataatttcg gtgtaaaccg tttgcgggca ggaatagccg caccacagtc gccctgcaat
     3241 cgtcgtccac caaaacagcc cgaaggcgca aatcatcagc agcaaggcaa ggtaaatcaa
     3301 atcgcccacc cccaacgaca atccgaaaat gaagaaatgc cgttcgggga tattgaaaac
     3361 gacggcctgc ctgccgctcc agttgaacca cggaatgacg taaaacacaa actgcgtcgc
     3421 caatacggcg gcgatacgca gtttggcgaa ccgtccttcc gcctttttgg gatggatgcg
     3481 ttcgccttcg ggatggattt gaatcacgct ggctcgcgga tcgaatgttt tttttgcttt
     3541 cggcgcggct tttgtttgtt cggacgtgcc gattccggat gccggactgc cggcttggtt
     3601 ttccgtggtc attctgcatt ccttagattt ttgattgatg gtttgcccgt taccgccgcc
     3661 gtttgctttt cagacgtcat ttttcttgtt ttttaaggcg ttgtgtttca agttttgaga
     3721 aaatccgttt ttcccaaaat atatttccgc tattgtacaa ctttatgcgc cgtccggatg
     3781 tatggggcgg atacatttcc catccgcatc aaaacgcctg gattttacct taccgcccga
     3841 acaaaatccg aatacggtta aaaaaaaaga ctaaaaaaac cgacaccccc atatcggcag
     3901 aaccgacggc gcaagctcat aaacaaacgc tatcgacaat ccggcacaca atctataact
     3961 ttttatttca aaaggaataa tggcaggctt cgcccgcaaa tcgaaaatcc ttccccgcct
     4021 gtcccctgcc gccgccttcc cacgcgtccg cccttttctt gaaagcataa gcgaatcggg
     4081 cgataatcaa cgctttccga ttatccactt atctgaaaca ccagcaagga aaatacaaaa
     4141 tgtctcaact ggcaaacgca atccgcttcc tctcggccga tgccgttcaa aaagccaatt
     4201 ccggccaccc cggcgcgcct atgggtatgg cggaaatggc ggaaacattg tggacgaaat
     4261 tcctcaatca caaccccgcc aaccccaaat tctacaaccg cgaccgcttc gtcctctcca
     4321 acggccacgc gtctatgctg ttgtacagcc tgctgcacct gaccggctac aacctaagca
     4381 ttgaagactt gaaaaacttc cgccaactgc acagcaaaac ccccggccat cccgaatacg
     4441 gctacaccga cggcgtggaa accacgaccg gcccgttggg gcaagggatt gccaacgcgg
     4501 tgggtatggc attggcagaa aaaatccttg ccgccgaatt taataaagac ggtttgaaca
     4561 tcgtcgatca ttacacctac gtctttatgg gcgacggctg tctgatggaa ggcgtatcgc
     4621 acgaagcctg ttcgctcgcc ggcaccttgg gcttgggcaa actgattgtt ttatatgatg
     4681 acaacaatat ttccattgat ggtaaagtgg acggctggtt taccgaaaac atcccgcaac
     4741 gctttgaaag ctacggctgg cacgtcgttc ctaacgtaaa tggtcatgac accgccgcca
     4801 ttcaagccgc catcgaagcc gcacgtgccg aaaccggcaa accgtccatc atctgctgta
     4861 aaaccttaat cggcaaaggc agtgccaata aagaaggcag ccacaaaacc cacggcgcac
     4921 ctttgggtgc ggacgaaatc gaagccacgc gcaaacattt gggctggact taccctgcct
     4981 ttgaaatccc gcaagaaatt tacgatgcgt ggaatgccaa agaaaaaggc gcgaaactgg
     5041 aagccgaatg ggacaaactg ttcgcgcaat accaagccaa atatcctgcc gaagccgcag
     5101 aatttgtgcg ccgtatggat aaaaaactgc cggaaaactt tgacgggtac gttcagacgg
     5161 cattgaaaga agtgtgcgcc aaagccgaaa ccgtcgccac ccgcaaagcc agccaaaaca
     5221 gcatcgaaat cttggcaaaa gagctgcccg aactggtggg cggctctgcc gacctgaccc
     5281 cgtccaatct gaccgactgg tcaaacagcg tctccgttac ccgcgacaaa ggcggcaact
     5341 acatccacta cggcgtgcgc gagttcggca tgggtgcgat tataaacggc ttggtattgc
     5401 acggcggcgt gaaacccttt ggcgcgacct tcctgatgtt cagcgaatac gagcgcaacg
     5461 ccctgcgtat ggcggcactg atgaaaatca accccgtgtt cgtgttcacc cacgattcca
     5521 tcggtttggg cgaagacggc ccaacccacc agccgattga gcaaaccgcc accctgcgcc
     5581 tgattccgaa tatggacgta tggcggccgt gcgacaccgc cgaatccttg gtcgcttggg
     5641 cagaagccgt caaagccgcc gatcacccgt cctgcctgat tttcagccgt caaaacctga
     5701 aattccaagc gcgcagcgaa caacaactga acgacatcaa acgcggcggc tacgtcatca
     5761 gcgaagccca aggcaacgcc caagccgtca tcattgccac cggttccgaa gtcgagctgg
     5821 ctttggaagc gcaaaaagca ctcgccgcgc aaaacatcgc cgtgcgcgtc gtttccatgc
     5881 cgtccaccaa cgtattcgac cgccaagaca ccgcctatca agccgccgtc ctgcccgaag
     5941 gcctgccgcg cattgccgta gaagccggac acgccgacgg ctggtacaaa tacgtcggcc
     6001 tgaacggcgc agtcgtcggc atcaaccgct tcggcgaatc cgcccctgcc gatctgctgt
     6061 tcaaagaatt cggctttacc gtggacaatg tggtcgatac agtgaaatca gtgctgtaac
     6121 cccacaccta aacaaatgcc gtctgaaacc aattagggct tcagacggca tttgtttgcc
     6181 ctctctcctt gatacttcct cattcctcca atctccatta taatatttgc gaatcactct
     6241 tattcacatt tcaaaaggag aaacgtatga acacccgcac cgaacacgac accatgggca
     6301 atgtcgaagt cccgtccgaa gcctattggg gcgcgcagac ccagcgcagc cgcaacaatt
     6361 tcaaaatcgg cggcgaaacc ctgccgcagc cgctgattta tgctttggcg ctggtgaaaa
     6421 aagccgctgc cgcgaccaat gtttccctcg gcaggattaa gccggaacag gcggatttga
     6481 ttacgcaggc ggcggatgat gtgttgaacg gcaggcttga cggacagttt cctttagtgg
     6541 tttggcagac cggctccggc acgcagtcca atatgaacat gaacgaagtg ctggcgaacc
     6601 gcgccaacga aatcgccggt acgggtttgg cggcgtatca gcccgtccat cccaacgacc
     6661 atgtgaacca cgcgcaatcg accaacgacg cattcccgac cgccatccac gttgccgccg
     6721 cgattgaaat caaccgccac ctcatccccg ccgtaaaagc cctgcgcgac accttggaca
     6781 aaaaagccca agccttcgcc cccatcgtca aaatcggccg cacccacttg caggacgcga
     6841 cgccgctgac tttaggccaa gaattttccg gctacgtttc ccagctcgac cacggcttag
     6901 gccgtctgaa tgacgcgctt aaagacttgt atgaacttgc tttgggcggt acggcggtcg
     6961 gcacgggttt gaacagccat cccgaatacg ccgaaaaagc cgccgccaaa ctcgccgaat
     7021 tgtccggctt gccgtttgtc agcgcgccga acaaatttga agccttgggc ggacgcgatg
     7081 ccgccgttgc cgcttcgggc gcattgaaaa cgctggcggc aagcctgaat aaaatcgcca
     7141 acgacatccg ctggctggca agcggcccgc gctgcggttt gggcgaaatc aaaatccccg
     7201 aaaacgagcc gggttcgtcc atcatgccgg gcaaagtcaa cccgacccaa tgcgaagcga
     7261 tgaccatggt gtgctgccaa gtgttcggca acgacgttac catcggcatg gcgggcgcgt
     7321 cgggcaattt cgagctgaac gtctatatgc ccgttatcgc ctacaacctc ttgcaatcca
     7381 tccgcctgtt gggcgacgcg tgcaacagct tcaacgaaca ctgcgccgtc ggcatcgaac
     7441 ccgtaccgga aaaaatcgac tatttcctgc accattccct gatgctggtt actgcgttaa
     7501 accgtaaaat cggctacgaa aacgccgcca aagtcgccaa aaccgcctac aaaaacaaca
     7561 aatcgttgcg cgaaaccgcc gttgagttgg gcttgctgac gggcgaagaa tttgacgaac
     7621 tggtcgttcc tgccgatatg gttcatccgc gctaatcttt ccctcaaata aaatgccgtc
     7681 tgaaacctcg ttcagacggc attttccgtt gcctgcgaac tagcggcgtt tgaacagcct
     7741 gtcccccacc gccgccgtaa ccgcaccccc gaccacgacc agtgcgccaa cataacccaa
     7801 accgttcata tccggcgcgg caaaagtatc aggcatcaca taatgcccga gcaaagaaaa
     7861 tattacggta aacacgggga gcaaggttgt taccgcgctg actttggaag cctcccaatg
     7921 tttcaacgcc tcgccgaacg agccgtaacc gattaacgta ttcaagcagc aatacgcaaa
     7981 acaaacccac gccaacgtac cgtccaaact tccgatgtgt gccggtttgg caaacggcag
     8041 gaacacggcg gcacttgccg cataaatcaa cagcagaatc tgttgcggtc cgaattgcgc
     8101 cgacagcagc ttttgcgcca cggcataaca cacccatgcc atactgcctg ccgcacacag
     8161 caacacgccc ttcgcatacg cgcccaaacc cgacaactcg ccgaatttat cgttaaaaaa
     8221 cataagcaaa ccggcaagca gcaaaaccaa gccgattttc tgagcggcag tcatccggtc
     8281 tttaaacacc aacacaccga caacaatcat cgtaaacggc gaaatctgcc acaaaacctg
     8341 cgtcgtggtc ggcgaaatat aatgcagccc ttgggcaatc agcacaaagt ttgccgaaat
     8401 gcccgccacg ccgagcagca gcagcctgaa tgagcaccaa gaaaaatccc gccacttcgg
     8461 cagccgcccg cccaatgcca gcaaaacaaa caataccgcc gccgccacgg taaaacgcac
     8521 ccacaccagc gtcggcgcat cgacaaactt caatacctgc cgcacggcaa tcggcagcgt
     8581 tccccacgtc atcgccgcca aaagtgccaa cgcgaagcct aggagcggcc tttggttttc
     8641 catcctgatt ttcctatttt taaacaaccg tattgccgga cgatgccggt ttgccgcatc
     8701 gggcaatgat ggttcaagcg tttgacgttt gattccaacc ctttgatttc aaacaaaccg
     8761 gctgaagctc ggctattgct tcgcgctatt tgaaaacacc gcctgaattt taaaatatag
     8821 tggattaaca
//
LOCUS       DAWFAD010000080         8603 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWFAD010000080 DAWFAD010000000
VERSION     DAWFAD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.080"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(280..3132)
                     /gene="gcvP"
                     /locus_tag="WGO49_001346"
     CDS             complement(280..3132)
                     /gene="gcvP"
                     /locus_tag="WGO49_001346"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ0306451.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDEFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDARVYPQTLDVMKTRAKYFGFELVVSDFAKADDGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGINVVHKVFFDTVTVDFGNK
                     EKADQVFAAALESGYNLRRVNDTQVAAAFHETSACEDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQDDILQHPVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKSITGFDAISFQPNSGAQGE
                     YSGMLAIRRYQEAQGEAQRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3163..3522)
                     /locus_tag="WGO49_001347"
     CDS             complement(3163..3522)
                     /locus_tag="WGO49_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306452.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRRASLMAMLPL
                     FVVILVLLNIVFPLLAHPLAWLVPAGFMVLVGGFPLSLPLVALLVLTCCILAHCPPLS
                     RLLCCPCPNHPMSKNSA"
     gene            3470..3700
                     /locus_tag="WGO49_001348"
     CDS             3470..3700
                     /locus_tag="WGO49_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0306453.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKTPYRRNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            complement(3693..4334)
                     /locus_tag="WGO49_001349"
     CDS             complement(3693..4334)
                     /locus_tag="WGO49_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ0306454.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4324..4875)
                     /locus_tag="WGO49_001350"
     CDS             complement(4324..4875)
                     /locus_tag="WGO49_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ0306455.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLNWTLMLKKRQAFQTAFEGFDIDTVAAFDDTDRERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDTHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4879..5397)
                     /locus_tag="WGO49_001351"
     CDS             complement(4879..5397)
                     /locus_tag="WGO49_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ0306456.1"
                     /translation="MRLFPIAAALSLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGQVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFIPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5397..7037)
                     /gene="pqiB"
                     /locus_tag="WGO49_001352"
     CDS             complement(5397..7037)
                     /gene="pqiB"
                     /locus_tag="WGO49_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ0306457.1"
                     /translation="MTDNSPPPNGHAQAHVRKNNTFLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYADDTVIATQGGGLDDLQVKLADLLDKFGKLPLDKTVSGLN
                     ASLAELKSTLKSINTLSNNPQTQNIPNELNQTLKELRTTLQGVSPQSPIYGDVQNTLQ
                     SLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7030..8490)
                     /locus_tag="WGO49_001353"
     CDS             complement(7030..8490)
                     /locus_tag="WGO49_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ0306458.1"
                     /translation="MGSAVGGQPLEKIFLPDDKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDRGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDSAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPTGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGIAFNETEKHD"
BASE COUNT         1926 a         2199 c         2431 g         2047 t
ORIGIN      
        1 gaggcaacgc tgtactggtt tttgttaatc cactatattt gtttgtccgt gtggaaatga
       61 catcaaaggg tgtgaatagg cttttcatct ttgaaatctg tttgcacgat cggatgaata
      121 aaaagtagga tgataaaaaa gctgatgtat ggatactggt ttaactcatt tgttttaaag
      181 ttatagtcga gatacgtgta ttggtgcaac cagtctgccg ttgacgaaaa tgccgtctga
      241 aaatgtttca gacggcattt gtcttcgggg tttcaacggt cagtcttcat aattttccat
      301 cggtgggcag ctgcacacga gattgcggtc gccgtacacg tcgtccacac ggttgacgct
      361 cggccagaat ttgtgttcgc ggacgaatgg caacggaaag acggcttctt cacgggagta
      421 cggatgcgcc cattcgccgg ttacatcgga tgcggtatgc ggagcgttga ccagcggatt
      481 gtcctctttc ggccattcgc cgcgcccgac tttcagcact tcctgtttga tttgtttcag
      541 ggcggcgatg aagcggtcga gttcggcttt gctttcgctc tcggtcggct cgatcatcag
      601 cgtaccggca acggggaagg aaaccgtcgg ggcgtggaag ccgtagtcca tcaggcgttt
      661 ggcgatgtcg gtttcagtga tgccgctttc ggctttgagc ggacgcaaat cgacgataca
      721 ttcatgcgcg acgcggccgt ttttgcctgt atacagaatc ggatagtctt cgctcaggcg
      781 tttggcaacg tagttggcgt tgagcaatgc ccagcgtgtc gcctgttcca tgccttgttt
      841 gcccatcatg gtcaggtaca tccaagtaat cggcagtatg gacgcagaac cgaaggccgc
      901 agcagcaacg gcggtttggt cggcactggc gctgtgggtg tcggtcaaag cgtgtcccgg
      961 tgcgaacgga gccagatggg ctttcaagcc aatcggaccc atgcccggac cgccgccgcc
     1021 gtgagggata cagaaggttt tgtgcaggtt catgtgcaac acgtccgcgc cgacttcggc
     1081 aggctgcatg atgccgattt gggcgttgag gttggcgccg tccatgtaaa cctgtccgcc
     1141 gttttcatgg acgatgcggc agatgtcgcg gatgccttct tcgtacacgc cgtgggtgga
     1201 cggataggta atcatgatgg cggacaaagc gtcgcggtgt tgctcggctt tggctttcaa
     1261 atcgtcgatg ttgacgttgc cgtgttcgtc ggtatcgacg acgacgactt tcaaaccgag
     1321 catggcggcg gtggcggggt tggtgccgtg ggcggatttg ggaatcagac agatgttgcg
     1381 ctgcgcttcg ccttgcgctt cctgatagcg gcggatggca agcataccgc tgtattcgcc
     1441 ctgcgcgccg gagttgggct ggaaggaaat tgcatcaaaa ccggtgatgg atttcaggct
     1501 gttttccata tcggcgagca attcgcggta gccggcggtt tgcgcttcag gagcgtaagg
     1561 atggatgtcg gagaactccg cccaagtaat cggcaacatt tccgcagtcg cgttgagttt
     1621 catggtgcag ctgcccaatg aaatcatgct gcggttcatc gccaagtcgc ggtcttcgag
     1681 ttttttcagg tagcgcagca tttcgtgttc ggtgtggtaa cggttgaaca cgggatgttg
     1741 cagaatgtcg tcttgacgca gcagttcggc gttcagacgg cctttgacat catcggcaaa
     1801 tgcgaacgta tccttgccgg taaacgcgcg gtacaaatca accaaatctt cgcatgccga
     1861 tgtttcgtgg aatgcagccg caacttgagt atcgttaacg cggcgcaggt tatagcccga
     1921 ttccaaagca gcggcaaaca cttggtctgc tttctctttg ttgccgaaat cgacggtaac
     1981 ggtatcgaag aagactttgt gaaccacatt aatgccgtct gaaaccagcg catcggcaaa
     2041 agcagaagcc agcgcgtgaa tgcggttggc gatgcgtttc acgccttcag ggccgtggta
     2101 aacggcatac atacccgcca aattcgccag caatgcttgt gcggtacaaa tattggacgt
     2161 ggctttttcg cggcggatgt gttgctcgcg ggtggacaac gccatgcgca aggcaggttt
     2221 gcctgatgcg tctttggata cgccgatgat gcggccaggg gcggaacgtt tgaacgcgtc
     2281 tttaaacgcg aaataagcgg cgtgcggccc gccgaagccc ataggcacgc cgaaacgttg
     2341 ggtgttgccc aaagcaatat ccgctcccaa ttcggcaggt gatttcagta aaaccaagct
     2401 catgatgtcg gcggcaacag caacgatcgt gccttttgct ttcagacgac cgataacatc
     2461 ctgcaagtct tgcacatcgc cgtctttgcc gacgtattgg aacagcgcgc cgaagtattc
     2521 gccgtcatcc gctttggcaa aatcgctgac caccagctcg aagccgaaat atttggcgcg
     2581 ggttttcatc acgtccaaag tttgcggata cacgcgcgca tccacaaaga aacgctcgga
     2641 tttcaccttg cccacgcggt gcgccatcgc catcgcttca gccgcagcag ttgcctcgtc
     2701 cagcaaagac gcgcccgcca cagggaaacc ggtcaaatcg atacacactt gttggaagtt
     2761 taacaacgct tccaaacgac cctgggcaat ttccgcctga tacggcgtgt aggcggtgta
     2821 ccaacccgga ttttccaaca cgttgcgcaa aatcacgttc ggcacgcggg tcgggtaata
     2881 gcccaaaccg atataggact tgttgatcac gtttttcgac gcaatgcctt tcaatttcgc
     2941 caacgcgtcc gcttcggtca gggcatcggg caaatcgagt tcggacggca tacggatgct
     3001 ttgcggcacg gtattgccga caaattcgtc catgcttttc tcgccgacag cggcaagcaa
     3061 cgccgcttcg tcgccgaaac tcaaatggcg cgcggcaaat tcgttcgggt tgaacaattc
     3121 agatagtttc atttctactc cttcgggttt gtagggctgc catcaggcag aatttttaga
     3181 catcggatga ttcgggcaag ggcagcacaa aagacgggat aatggcggac aatgcgccag
     3241 aatgcagcag gtcaggacaa gcagcgccac aagcggcagc gataagggaa agccgccgac
     3301 caaaaccatg aaacctgcag gcaccagcca agccaaggga tgcgccagaa gcggaaaaac
     3361 aatattgagc agaaccaaaa tcacaacaaa cagcggcagc atcgccatca ggctggcacg
     3421 cctgtatgtt gcgtacgggt cggatgcttc ttcaggttcg caatattcca tgatttcagc
     3481 ctgtcctggc tttatccaca gcaggctcgg acggtcggtc atgccgtccg aaccggcagg
     3541 ccgtcttttt caaaaaacgc cataccgccg caacttcatg cggctgtatg gcgtaggttt
     3601 tgcacttcag acggcatatg cgtcccgaag cagtttggtg ttccagtcgg ttcatgatta
     3661 tataaacaac ttgggtgcgg ataaaccttt gtttatctaa acccctgttt tctcaaaaat
     3721 tgattcaaat cttcggcaaa tttccgatag cctttgccgt tggcgtggat ttggtcggat
     3781 ttcagattat tatcgcccaa aatttccgcc cacgcgccgc cgaacagcgg aatgccgtat
     3841 tcctcggaca aatcctcata cagcggatga tcgctcaaat gcccgaacaa cgcgcccagt
     3901 gtgatgtgcg gcacgccgac gaggacggcg gggatgtttt ccttctgcac ggtttcgatg
     3961 attttcgcga tattggcgcg ggtctgctcc ttgggaactt tgcgcagaaa gtcgttgccg
     4021 cctatgccga caatcacaag cttgggtttg cgtgccaaca gcgcgggcag gcgcgacagg
     4081 gcttgggcgg atgtatcgcc cgatacgccg ccgttgacaa tattccaacc cgtcagtttt
     4141 tgcagttgcg cggggtagga ttcgccaggg tttgcgccgt agccgaaggt aagcgaatcg
     4201 cccaaggcaa gtacggtgct tccttcggga attttggcgt gggttcgggc ggattttctg
     4261 ccgcaggcgg taaggagcaa cgcgcctgcg ccgaggagga aggttcttct gttcattggt
     4321 ttttcagatg gcatcgagcc atttcgggtg gtatttcagg gtttcacggt aaaccggaca
     4381 gctttcggcg tgcgcgcctt tgaggtagcc ggtactcatc agaaattcgc cgacgatttc
     4441 gccgccgacg aatttgaaat gttttttaaa gagtttaacc cattcgtctt tgcttcgcgg
     4501 atggtgcgtg tcgagccagt tcttgaacga gccgtattct tgttgcaacg cttggatttg
     4561 ccgtgcattg aaaatggcgg catcgatttt caggcggttg cggacaatgc ccgcgtcagc
     4621 aagcaggcgt tcgcggtcgg tgtcgtcgaa ggcggcaacc gtatcgatgt cgaaaccttc
     4681 aaatgccgtc tgaaacgcct gccgcttctt cagcatcagc gtccagttta atcctgcctg
     4741 attgatttcc aacaccagcc gctcaaacaa ttcattgtcg tcctcaatcg gaaaaccgta
     4801 ttgcgtgtcg tggtaatgtt tgttcgggtt atcggtgttt tcgggaagtg aggcggcaaa
     4861 ttcgcaatag ttcacgtttt actcgaccat ctgttgcgcc gcctgtttca gtccctgttc
     4921 gagtgcggcg gtcatcgcgg cgtagccgtc accctgctgt tcggtttcga tatggaaggg
     4981 tctgttcgta ccgtcgggta ggacggcgta gccgctgatg agggttttgc ccgtgtagct
     5041 gccttggaat gcgtcaatat agaccgtcca tttttcggta ctgccgctgc gtgaggcagg
     5101 aataaagatg cgtgtgctgt ccaaacggtt gaatgcattg ctcaacgccg cttcgagcat
     5161 atcgtccaag gtgtccgccc aaacgtggtt ttgtgcggtg ttgaggcggt aggggtcggt
     5221 ttgatagacc tgtccgccgc gtttgagcgg ttcggcaaga cggacttcga cggcagtttc
     5281 gccgccttgc gttgcaggac ggatgtagcg gctgtcgggc aacacgaaat attgtgtgct
     5341 ttgcacagta ccgcaggcgg caagcgacag ggcggcggca atcgggaaaa ggcgcattat
     5401 cggcttcctt tcgggatagg gtctttgctg ctgctgttga aaatcagcgc gttgggtttt
     5461 tctttcaaag tgttaatgac gggttgaacg tcttttaagg ttttgtccaa actttgcagc
     5521 gtattttgta cgtcgccgta gataggcgat tgcggcgata cgccttgcag ggttgtgcgc
     5581 aactctttca gggtttggtt caactcgttc ggaatgtttt gcgtttgcgg attattgctt
     5641 agcgtgttaa tggatttcag cgtggatttg agttcggcga gcgaagcgtt taaacctgaa
     5701 acggttttgt ctaaaggcag tttgccgaat ttgtccagca aatccgccaa tttgacctgt
     5761 aagtcgtcca aaccgccgcc ttgtgtagcg atgacggtat cgtctgcata aacggtatgc
     5821 ggtcgcagct tgggcgaaga cgaaggctga tcgttcaact caatcatttt gctgccggtc
     5881 agcaggttgt tgctggagat ggtggcggtc aggcctttgt ttaaagccgt ctgaaattgt
     5941 tgtttccaat gctctttgct ttgttcgtcg gcattgattt ccaaacggga aggctcgatg
     6001 cggatgcgta cgggaatcca gccgttttca aataaatgca ggctgtcgtt gcggtcaaaa
     6061 taagggacat cggaaaccac gccgacattc agccctttgt actcgacggg cgaaccgacg
     6121 gtcaggccgc gcacggattg tttgaaaaac gcggtgtagt acagcgagcg gtcgtcaggc
     6181 aggttggcga cttcgctgcg gctgtcgtaa agcgtgaagc tgtcttcgct tttgacgttt
     6241 ttactgtttt tggttttcgg cgaatcaaat gaaatcgcgc ccgacagcag ggcaggcaga
     6301 ggggcggaat tgagtttgat gccgctgcct gtggtttcga tattgatgcc gctttccagc
     6361 caaaaacggc tggcggaatg aatcagtttg tcgttggggc tttggatgaa gatggtgtaa
     6421 tgcacgcttt ggtcggacgg gtcgaaatgc gcgctttcga cttgcccgac cataaagttt
     6481 tcatacaaaa cagggctgtt gacgttgagg atgcggtcgt ttttaccaat caaattcaag
     6541 cgcagcccgc tttgcccgat ggcggtaacg ggcggaatgt cctgcacttg gaacacgtct
     6601 tttgcctcgt cgcttttgcc gggtgtaaaa gcgatgtacg aacccgaaag cagcgtaccc
     6661 aaaccggtta cgccgctttg gtcgatacgc ggcttgacca cccaaaactg ggtatcgctg
     6721 cggatgaggc cggatacgtc cgcattgagt tgggcggtta cttccacgcc tttttggtcg
     6781 tcgcgcagtt tgattcgggt aacgcgtccg acatcgatgc tcaatacttt gatgaccgta
     6841 ttgttgacct caatgccttc cgcgctgtcc atcaagagcg taaccacagg ccccctgttg
     6901 cggatttcct taatccacag ccagccgccg gcaatcagcg cgatcagcgg aaccagccag
     6961 acggcagaga ggaaggtgtt gtttttgcgg acgtgtgctt gggcgtgtcc gtttggagga
     7021 gggctgttgt cagtcatgtt tttccgtttc gttgaaagca atgccgtctg aagcgcgttt
     7081 gtcccaaagc aggcgcgggt cgaaataata ggcggacagc atcgtcagaa tcacgaccag
     7141 gcagaaatag actgccgcac tgcccggaat gacgcgcgcg gcataagtgt ggaacgaaca
     7201 cattaaaata ataatcacaa aaatatcaat catcgaccag cggccgaccg cttcggtgat
     7261 gcggtagagg tgtgacaatt tctttgcgcc tgtcggcaaa gcgaagcggg cggacgcaat
     7321 taaaaccgac attgccgcaa tcttcagcac cggcaccaaa atactcgcgc tgaaaataac
     7381 cgccgcaatc agcctgtcgc cctcgtccca catataagcg atgccgttaa ggatggtatt
     7441 gacctccgtg gcggcaggat tggacgaaat cataatcggc aggatattgg cagggaaata
     7501 caaaataacc gccgccgtca gaaacgccga cgaaatactc agacttttcg gccgtcggcg
     7561 gtacagttcc gcgccgcaca caccgcaggg ggattcggca ctgtcgcgga aatacaggca
     7621 gcggctgcaa caggttttgc cttccgatgc cgtctgaacc gcattatccc ccgtcagccg
     7681 cccgatttga aaatacaccc aatgctgggg aacggatacc gaagtccgaa tcagcataac
     7741 cgacagcgcg aacatcagat aaaacgccga cccgaagcga acctctgcca cagacgagag
     7801 cttgatatac gccaccagcg tggaaacaaa aaacacatcc accatcatcg cctgcctcaa
     7861 gcgcaccatc acacgcgttg ccaaacgcag cgcaggatac acccgtttcc gtatcagcgc
     7921 ggcatagaca tacaggcaca gcagcagaaa cagaaccggc gcgccgaagg tcagcacaaa
     7981 catcacttcg gccaaaaaac cataatcctg aaacaccatc aggcgcatca tctcgggcag
     8041 cgaaaggacg gatgccgcac ccggtatccc gacctcgata tacgtcatac cgtaagcaaa
     8101 cgccatcaaa atcagcgaag ccgccgcata agcgggcggg gcggaaaaag gatgcctgcc
     8161 caccctgaag agcttatagc cgcagcgcgg gcagaacgcc gcctcccccc tgtccaaccg
     8221 aggcacatcc gcgcggcagc cgcattccgg gcaatctacc gtatgcggcg gtagggcttc
     8281 gtcgcggcag ttgccgcgaa ggctgtgggg gatattttcg gcaaacggct tcatcgggaa
     8341 agtggcaaat ataagtggcg gcattataaa ggaaaaaagg cgatacaccc aatcaggatg
     8401 caaaaccggc aggttcggca gtatgaacaa acccttgtcg tccggcaaaa atattttttc
     8461 gagcggctgt ccgccgactg ccgaacccat ttgataaaat cccgattctg taaagaatag
     8521 taaaatacct gcttaaaaac atcacgcaaa tgatattttt tccaaaaata tgtgataata
     8581 aatttcgttt atgattcttt att
//
LOCUS       DAWFAD010000081         8552 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWFAD010000081 DAWFAD010000000
VERSION     DAWFAD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.081"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1017..1787)
                     /gene="xth"
                     /locus_tag="WGO49_001354"
     CDS             complement(1017..1787)
                     /gene="xth"
                     /locus_tag="WGO49_001354"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0306459.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSAPEDVYIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWYEKIHCSSVERQWFQNLLDLGLTDSLRHIHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVAAEFDW"
     gene            complement(1847..2128)
                     /locus_tag="WGO49_001355"
     CDS             complement(1847..2128)
                     /locus_tag="WGO49_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0306460.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIVELAKSLSLSAT
                     AVSNHLNRLRVEGLVDFTRYHRIIEYRLVSEEAAAILHTVRDLENKRAA"
     gene            complement(2599..3480)
                     /gene="nadC"
                     /locus_tag="WGO49_001356"
     CDS             complement(2599..3480)
                     /gene="nadC"
                     /locus_tag="WGO49_001356"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ0306461.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSRENGVIAGMDLARLAFQTMDPSVRFQAEIQDGQTVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATALAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGPLTCVEIEVDTLAQLDEAIAAG
                     AKRILLDNMDDETLKEAANRCHTQTSHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(3615..4091)
                     /locus_tag="WGO49_001357"
     CDS             complement(3615..4091)
                     /locus_tag="WGO49_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306462.1"
                     /translation="MSYLILDDDMYLIYDFKKVMKREKEFIEKTRLLSLNDESIFGIK
                     KDKGLFASEEWWSNIDSGQIITRSVSGVISSIYEAGMERRNIPNSFSFIDGEGIVRNE
                     SMYMMNKSDRHLFCEGKKIVIFYAYNELKKSNQRKGDVIDLENNYVEQVIEMAISK"
     gene            complement(4088..4333)
                     /locus_tag="WGO49_001358"
     CDS             complement(4088..4333)
                     /locus_tag="WGO49_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306463.1"
                     /translation="MEVLKKIGTTGNSATGKLFPDLPRTNKGWTEKTQVQFKLEGQGK
                     PHVNKGKGVVNTGLGRAGGKETFEKQIIYFEKIGDVK"
     gene            complement(4452..5390)
                     /locus_tag="WGO49_001359"
     CDS             complement(4452..5390)
                     /locus_tag="WGO49_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306464.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYDYFPWEELRTDGRRRDAVVSRLRIWANSADTEEVRQKRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIAEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLYRFRDDVLPDRLISDIGLPLGSR"
     gene            5592..6704
                     /gene="nadA"
                     /locus_tag="WGO49_001360"
     CDS             5592..6704
                     /gene="nadA"
                     /locus_tag="WGO49_001360"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ0306465.1"
                     /translation="MQTAARRPFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKQKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEADTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKQFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6758..8266
                     /gene="nadB"
                     /locus_tag="WGO49_001361"
     CDS             6758..8266
                     /gene="nadB"
                     /locus_tag="WGO49_001361"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ0306466.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFCIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRAILSQGKPAIEWLLTQGVAFDQ
                     NNNDLHLTREGGHTCRRIAHVADYTGEAVMQSLIAQIRCRPNIRVCEQQMALDIQTES
                     GSACGLTVLDRRTQETHRIRASHTVLAGGGLGQIYAATTTPPECTGDAVAMAIRAGCA
                     VENLEFIQFHPTGLARPSENGRTFLISEAVRGEGGILTNQAGERFMPYYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISRQSAEFVRQHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAVPSQRLSESPTAEAGIFSDDLQNTFSRPVLQAFNQRHLGILRNDTGLRC
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            8449..>8552
                     /locus_tag="WGO49_001362"
     CDS             8449..>8552
                     /locus_tag="WGO49_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0306467.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPI"
BASE COUNT         2013 a         2284 c         2181 g         2074 t
ORIGIN      
        1 ctgatgcaga tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta
       61 caaatagtac ggcaacgctg tactggttta aatttaatcc actatatttt gtaatagctg
      121 ccattatgcc tactaggata tataccttct atataatcct tatttaaata aaaaccctta
      181 taaaaatctg tgttatctgt cgggattgaa aaaaagattt tatctttctc atcaggttta
      241 tatttgctat caatatcaaa aagagaataa ggattacaat gagttcgaca tactaggtaa
      301 aaatttatat cttggatcat gaaatattcg atatttatca ggatcgaaag atgctaaaaa
      361 ttcatcaata tcggtactgg atattttgtc atcactaata aacatcgcta gagatgggcg
      421 actttgattt tccatttgtt gattagtaaa ggatataaat actaacttgc cattgtcaaa
      481 ccacatagat ttatatagtg gattaaattt aaaccagtac gtcgttgcct cgccttgccg
      541 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatag
      601 ttattgcctt tgggagaatc aattctgaca gcacataaca taggttcatc tttacagtcg
      661 ttatattggg tttcatcaaa ctcaactgca tagcacaata aaggcaatag gaaaaaaagg
      721 agattaattt attactatac ataaggcagc ctgattattc attctaaaat aaatccggtt
      781 tttaatattt gattctgctc cggattatat acataataaa ttttactgct ccaaaatctg
      841 atattaaccc tatggatttc ataaattttt cagaaaaata ggctgtgctt gaacaatccg
      901 ccgactccgg ctgtttgtca gggaaaaggc aattattttc ggttagtcag cagcctatcc
      961 tattgaatca cttaaagata tggatgtttg tcaacgccat atcaaaacac ggtcttttac
     1021 caatcgaatt ctgccgccac gggcgcgtgg tcgctcggac gctccagcgc gcgcgtctcc
     1081 aaatcgacgc ggacatcctt caacgccgcc gccatcgcag gcgacaccaa aatatgatcg
     1141 atacgcaggc ccagtttgcg ttggaacatc gcgccgcgat aatcgaacca agtatagaac
     1201 gcgccttcgg gatggatatg gcgcaaactg tcggtcaatc ccaaatccag cagattttga
     1261 aaccactgcc gttcgacgga cgaacagtgg attttttcat accatttttc agggtcgtaa
     1321 cagtccgcat cggcaggcgc gatattgaaa tcgcccagca gcaccagttt gccgtagcgg
     1381 gtcatttcgt cgcggacaaa ctccgtcagt gcggcaaacc actgttcctt atatttgaat
     1441 ttggggctgt ccaaagcctc gccgttgacg caatagacat tgatgacgcg cacgccgccg
     1501 acggttgccg caatcacgcg ccgttgcgga tcgtccggca gcgcgggcag cccgatatac
     1561 acgtcttccg gcgcgctgcg gctgacaatt gccacgccgt tgtaggtttt ctgcccgctc
     1621 caaacacagt gccagcccat catctgcaag gcggcggcgg gaaacttgtc ctgatcgagc
     1681 ttgagttctt gtaaaacaag gatatccggc ggattatcgg caagcaggtt ttgcacctgc
     1741 ggcaaacgta cattgagcga attgacgttc caagtggtaa ttttcataac atttccgaaa
     1801 aatgctgtcc gaaacgttca gacggcaaaa ggaaaggatt ctaacactat gccgcgcgtt
     1861 tgttttccaa atcgcgaacc gtgtgcagaa tcgccgccgc ttcttcggaa accaggcggt
     1921 attcgataat gcggtggtaa cgcgtaaaat cgactaaacc ttccacgcgc aggcggttca
     1981 aatggttgga aactgcggta gccgacaggg ataaggattt tgccagttcg acgatattgc
     2041 gttcgctgtc caacagttgg atcagtatcg ccatacgttc gggatgcgcc ataagtttga
     2101 gtatggtgtg gagcggtatt gtgttcatcg gtattcctaa aaccttttcc gcccgccaag
     2161 cggattgacg ggcggcagat atggatgatt ttgattatct ttataaattg tattataaat
     2221 caattgttta tggctcattg tacaggattg tatgttcctt ttcccactat ttttgcaaac
     2281 aagcgtaaag acaggctttc agacggcata aaactgccgc gcccgtcctt ttccctatcc
     2341 tgccgatgcg tcggaatcat ccgcattatt caaaagatac cttacaagcg ccgcatacct
     2401 tgccctgccg atgcctccgc gctgtcgggc aaacaaaata cctgcctgaa atgctgtttt
     2461 ttctaaagca tcacgacctt cacgaaaaat gccgtctgaa gaatctttca gacggcattt
     2521 cctccctgag gcaataaccg gattacggtt aagcggttcc ttgcctgaca caccaaacag
     2581 cagccctcac cctaaaactc acgccacgaa atccaaacct atatccaacg agcggctgct
     2641 gtgggtcaga tagccgaggg cgatgccgtc cacgccggtt tgcgccacgc gcttcagtcg
     2701 gtcgaagccg atgccgcccg atgcttcgca atagacggtg tgggggtggg aggtttgcgt
     2761 gtggcagcgg tttgccgctt ctttcagggt ttcgtcgtcc atgttgtcca gcaaaatccg
     2821 ttttgcgccc gccgcgatgg cttcgtccag ttgtgccaac gtgtccactt cgatttccac
     2881 gcaagtcaac ggtccgaccg cctgttttgc ctgacgaacg gcttgggcga tgctgccgca
     2941 ataggcgagg tggttgtctt tgatgagtac ggcgtcgtcc aaacccatgc ggtggttcac
     3001 gccgccgcct gccctgacgg cgtatttttg caggacgcgc agcaggggga tggttttgcg
     3061 gctgcacacg atgtctgtac cgtattcggc gacttcggca acggcaagcg cggtggcggt
     3121 ggcgatgccg ctcaagtgcg tgaggtagtt gagcgcggtg cgttcggcgg cgagcagggc
     3181 gcgggcgttg ccttcgacgg cggcaagcgt ctgacctgcg cggacggttt gcccgtcttg
     3241 gatttcggct tggaaacgga cggacggatc catcgtctga aaggcgaggc gcgccaagtc
     3301 catgccggcg atgacgccgt tttcgcggct gacgaggaag agtttggcgg ttttgtcagg
     3361 cgcgatgacg gcggcggacg taatgtcgcc gcgcctgccc aaatcttcgc tcaaggcttg
     3421 ttcgaccatg gggcgcaaca gggtgtcggg cagggggaag agggtgtttt cagacggcat
     3481 ggggtttcct ttgttgaaaa atattggtgt tgcaccgatg gggcaaaact tcattttata
     3541 gtggattaac aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga acaattctct
     3601 ccggcgtcat ctgattattt tgaaattgcc atttcaatga cttgctcaac atagttgttt
     3661 tccaaatcaa ttacgtctcc ttttctttga tttgattttt ttagttcatt atatgcataa
     3721 aaaatgacga ttttcttccc ttcacaaaat aaatgtctgt cggatttatt catcatatac
     3781 atactttcat tccttacgat gccttcacca tctataaaac taaaactatt cggtatattt
     3841 cttcgttcca ttcccgcttc atatattgac gatatcactc cggaaacgct tctggtgata
     3901 atctgtccgg aatcaatatt actccaccat tcctctgatg caaataagcc tttgtctttt
     3961 ttgatgccaa agatactttc gtcatttaat gataataatc ttgtcttttc aataaattct
     4021 ttttctcttt tcatgacttt tttaaaatca tagattaaat acatatcatc atccaaaatc
     4081 aaataactca ttttacgtct ccaatttttt caaaataaat aatctgtttc tcaaaagttt
     4141 ctttaccgcc agccctaccc agtcctgtat tgactacccc tttcccttta ttcacatgtg
     4201 gttttccttg gccttcaagc ttgaattgca cttgtgtttt ttcagtccat cccttatttg
     4261 tgcgaggtaa atctggaaaa agtttgcctg tggctgagtt gcctgtggtg ccaatctttt
     4321 ttaaaacttc catagttcct ggtaaggtgt tcaagcacca agtgaatcgg ttccgtacta
     4381 tctgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccgc tataggtcgt
     4441 ctgaaaacgg gctaacggct gcccagcggc agtccgatgt ccgaaatcag cctgtcgggt
     4501 aggacgtcgt cgcggaagcg gtaaagctgc gcgggacggc ctttgctgcc cgatacgccg
     4561 gtatccgacg gctcgatgag gttttgctgc tgaatctggc ggcggaagtt ttgtttgtgc
     4621 agcaatctgc cgctgatggc ttcgacgctg ttttgcagtt gcagcagcgt gaattcgggc
     4681 ggcatcaatt caaaaatcac ggggcggtat ttgattttgg cgcgcaggcg ggacagggcg
     4741 gttgccagca cgcggcggtg gtcgtggcgc atgggctgcc ccgtgagcgc gaagtcgaag
     4801 tttgcctgcg gctcggcggc ttccgctatc aggccgcttt catacagcat ttcatagcgt
     4861 tgcaaaacgt attcttccga ccagttttcc ggttcgaccc cccaacacaa atgaatgcgc
     4921 ttgagccgct tttggcgcac ttcctccgtg tccgccgagt ttgcccaaat gcgcaggcgg
     4981 ctgacaacgg cgtcgcgccg cctgccgtcg gtgcgcaact cttcccacgg gaaatagtcg
     5041 tagcagtctt gccatttcgc gtcgggatgc aggatgctgt cggctgcctc gcgcaccaac
     5101 cccaaatagc tgacgtacag aacgggcata ccgtgttcgt tacggcggtg ggtatcgaca
     5161 aaggtgtaaa gctgttccac atagcccatg ggctgcgaag tttgcttggc gacccacagc
     5221 tttacgcctg cctgcaggga attgcgcagg ggggagagcg ggccgttggg caggagcgtg
     5281 ccttgggcga cggtcaatac ccgcagtccg ccgtcggtaa cggcaatcaa tacgggaacc
     5341 agctcgacga tgctttgcgg cggtgcttcg gcttcggggt aggcatccat gctcggcttt
     5401 ctgcgggtgg gaaaggcttt attgtaaacc aaagcggttt gtcggaacga agcgggtcgg
     5461 ttttgcggta tcgcaaacct gttttcagac gaccttgctt attatactca acttgagcat
     5521 aatataaaaa cagtttgaaa agcagacggc aaaccttcat gccgtctgaa aaccagctac
     5581 aaggaaacat catgcaaacc gccgcccgcc gcccgttcga ctacgatatg cccctcatcc
     5641 agacaccgac ttccgcctgc caaatccgtc aggcgtgggc gaaggttgcc gacacgcccg
     5701 accgcgagac ggcaggccgt ctgaaagacg aaatcaaggc tttgctgaaa cagaaaaacg
     5761 cagtcttggt ggcgcattat tacgttgatc cgctgattca ggatttggct ttggagacgg
     5821 gcggatgcgt gggcgattcg ctggaaatgg cgcgcttcgg cgcggaacac gaagccgata
     5881 cattggtggt ggcgggcgtg cgcttcatgg gagagagcgc gaaaatcctc tgccctgaaa
     5941 aaacggtgct gatgcctgat ttggaagcgg aatgttcttt ggatttgggc tgtccggaag
     6001 aggcgttttc ggcgttttgc gaccaacacc ccgaccgcac ggtggtggtg tacgccaaca
     6061 cttccgccgc cgtgaaagcg cgtgccgatt gggtggtaac gtcttcggtg gcgttggaaa
     6121 tcgtgtcgta tctgaaatcg cgcggcgaga aactgatttg gggacccgac cgccacctcg
     6181 gcgactacat ccgccgcgaa acgggcgcgg atatgctgtt gtggcagggt tcgtgcatcg
     6241 tccacaacga attcaaaggg caggagctgg cggcgttgaa ggcggagcat cccgacgcgg
     6301 tggtgctggt tcatccagaa tcgccgcaaa gcgtcatcga actgggcgac gtggtcggct
     6361 cgaccagcaa actgctgaaa gccgccgtat cgcgccctga aaaaaaattc atcgtggcga
     6421 ccgatttggg catcctgcac gaaatgcaaa agcaggcgcc cgacaaacaa tttatcgccg
     6481 ccccgacggc gggcaacggc ggaagctgca aaagctgcgc gttctgcccg tggatggcga
     6541 tgaattcgct gggtgggatc aaatacgccc tgacaagcgg acgtaacgaa atcctgttgg
     6601 acaggaagct gggcgaagcc gccaaactgc ctttgcagcg tatgctcgac ttcgcggcag
     6661 gactcaaacg cggggatgtg ttcaacggca tgggtcccgc ctgatttacc gtcaatattg
     6721 ccgtttcaga cgacctctca aacaaggaca acaccatatg caaaccgatt gcgacgtatt
     6781 gattgccgga aacgggctgg cggcactgac gctcgccctg tcgctgcctg aatcgttctg
     6841 catcgtcatt ttgtgcaaaa accggctgga cgacaccgcc agccgtcatg cgcaaggcgg
     6901 gattgcggcg gcgtggtcgg gagaggacga catcgaaaaa cacgtcgccg atactttgga
     6961 agcgggcgcg ggtttatgcg acgaagccgc cgtccgcgcc atcctgtcgc agggcaaacc
     7021 ggcaatcgaa tggctgttga cgcagggcgt agcgttcgac caaaataata acgacctgca
     7081 cctgacgcgc gaaggcgggc atacctgccg ccgaatcgcc cacgttgccg actacacggg
     7141 cgaagccgtc atgcagagcc tgattgccca aatacgctgc cgcccgaaca tccgagtttg
     7201 cgagcagcag atggcgttgg acatccaaac cgaatcaggc tcagcgtgcg gactgaccgt
     7261 cctcgaccgc cgaacgcaag aaacccaccg catccgcgcc agccataccg tactcgcagg
     7321 cggcggcttg ggacagattt atgccgccac caccacgccg cccgaatgca caggcgacgc
     7381 cgtcgctatg gcgatacgcg caggctgcgc agtcgaaaac ctcgaattta tccaattcca
     7441 ccccacaggc ttggcaaggc cgtctgaaaa cggccgcacc ttcctgattt ccgaagccgt
     7501 gcgcggcgaa ggcggcatcc tgactaacca agcgggcgaa cggtttatgc cgtattacga
     7561 ccgccgcgcc gaactcgcgc cgcgcgacat cgttgcccgc gccatcgccg ccgaaatcgc
     7621 caaacagacg caagacttcg tctcgctcga catcagccgc caatccgcag agttcgtccg
     7681 tcagcatttc ccgtccatcc atcggcactg cctgtcccaa tgcggtttgg acattacgcg
     7741 ccaagccatc cccgtccgcc ccgtgcagca ctacacctgc ggcggcatcc aaaccgaccc
     7801 ctgcggcaga acttccctgc cgcagctcta cgccttaggc gaaaccgcct gcaccggctt
     7861 gcacggagcc aaccgcctcg ccagcaactc cctgctcgaa tgcgtcgtta ccgccaggct
     7921 tgccgcccaa accatcgcgg acggacaagc attccaagca gtaccgtccc aaaggttgtc
     7981 tgaaagcccc accgccgaag caggtatctt ttcagacgac ctccaaaaca cattcagccg
     8041 tcccgtcctg caagcgttca accaacgcca tctcggcatc ctccgcaacg ataccggtct
     8101 acgctgcgcc atcgcccagc tgcggctttg gaagcaaaac caaaccgagc cgcacactgc
     8161 gtccgaatac gaaaaccgca acctgctcga atgcagcctc gccgtcgccc aagccgcata
     8221 caggcggaga cagaacatcg gcgcgcattt taatagtgat tgttaaggtt ggctggttag
     8281 aaaaaatcgt tcaaatgaat tttaatagca tggttaagag attggcattc agtttttagg
     8341 gttgcgtaag ttgggttatc aatctgttct aaaagcagaa aggccgagac ctttgcaaaa
     8401 ttccccaaaa tcccctaaat tcccaccaag ccatttaggg gattttccat gagcaccttc
     8461 ttccggcaaa ccgcacaagc catgattgcc aaacacatcg accgcttccc gctattgaag
     8521 ttggaccagg tgattgattg gcagccgatc ga
//
LOCUS       DAWFAD010000082         8500 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWFAD010000082 DAWFAD010000000
VERSION     DAWFAD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.082"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            83..1267
                     /gene="coaBC"
                     /locus_tag="WGO49_001363"
     CDS             83..1267
                     /gene="coaBC"
                     /locus_tag="WGO49_001363"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ0306468.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1334..3490)
                     /locus_tag="WGO49_001364"
     CDS             complement(1334..3490)
                     /locus_tag="WGO49_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0306469.1"
                     /translation="MPVPQPTAPYDHLTAEARTLLFRTASYLKPEEQVELEKAVAYAF
                     HAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLDQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3583..3789)
                     /locus_tag="WGO49_001365"
     CDS             complement(3583..3789)
                     /locus_tag="WGO49_001365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ0306470.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WGO49_001366"
     CDS             complement(3848..4465)
                     /gene="gmk"
                     /locus_tag="WGO49_001366"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ0306471.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4593..5192
                     /locus_tag="WGO49_001367"
     CDS             4593..5192
                     /locus_tag="WGO49_001367"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ0306472.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYHLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5238..6026)
                     /locus_tag="WGO49_001368"
     CDS             complement(5238..6026)
                     /locus_tag="WGO49_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ0306473.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVHEVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEREGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            6351..7706
                     /gene="yegQ"
                     /locus_tag="WGO49_001369"
     CDS             6351..7706
                     /gene="yegQ"
                     /locus_tag="WGO49_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ0306474.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLNVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDARLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHHTQDYQNYLTGHSTAKQSQYVGHVTEIDKNGWATIEVKNR
                     FAVGDSLEIIHPNGNQTIKLEQMTRKGQPVDVAPGNGIQVKIPNMRGKEKALIARVLN
                     P"
     gene            7840..8316
                     /locus_tag="WGO49_001370"
     CDS             7840..8316
                     /locus_tag="WGO49_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ0306475.1"
                     /translation="MPAVCQSGSAPKNAHTASAGTPAGESCKVIRDDTAGSGKNIIYR
                     CSNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQTGSFHVRVVMRANVVR"
BASE COUNT         2165 a         2327 c         1998 g         2010 t
ORIGIN      
        1 ttgtatccaa acggtatcta atgtatccac actttgttat agaatggctg ctgttttttc
       61 ttcgtaatta gaaattgtca aaatgggcaa acatattctt ttaggtgtaa cgggcagtat
      121 tgcggcgtat aagtcttgcg agttggttcg tctgctgaaa aaacaggggc attcggttac
      181 ggtggttatg agccgctcgg caactgaatt tgtttctccg ctgacttttc aggctttaag
      241 cggcaatcct gtcctgaccg acacgcacgg cggcaacggt tcaaacggta tggaacatat
      301 caacctgacc cggaatgcgg atgtttttct gattgcgccg gcaagtatga ataccgtggc
      361 aaaaatctgt aacggcgtgg cagataacct actgaccagt ctggcagccg cacggaaatg
      421 tccgcttgcc atcgctcccg cgatgaatgt ggaaatgtgg ctcaaccctg ccaaccaacg
      481 gaatatcgca caactggttt cagacggcat tactgtctat atgccgggct tgggcgaaca
      541 ggcttgcgga gaaaatggca tgggaaggat gccggaacct gccgaattgc tggatttgct
      601 tccggacttg tggacaccga aaattttaag gggcaagaaa atcttgatta ccgcaggtgc
      661 gacatttgaa gccattgacc ctgtcagggg catcacaaat acctccagcg gaaaaatggg
      721 ggtagctttg gcgcgggcgt gccgtgccgc cggtgcggaa gtcagcctga ttcacggaca
      781 gcttcaaacc gcgctgcctt tcggcatatc cgatacggtt caagccgtca gcgctgaaga
      841 tatgcatcgc gcagtgcatc gtttgattga aaaacaagat gcttttattt ctgttgccgc
      901 cgtctcagac tataaggtta aaaacaggag tacacaaaaa ttcaaaaaag ataaaaatgc
      961 caaaccgtta tccatcgaat tggatgagaa ccccgatatt ttggcttcta ttgcctcatt
     1021 gccgaacccg ccgttctgca tcggttttgc cgctgaaacg gagaatgtaa tggcatatgc
     1081 acgagaaaaa cgtattaaga aaaaaatacc ggtgatagtt gccaatgatg tttcaatcgc
     1141 gatgggcaaa ccgaccaacc ggattaccat tatcggggac gacggggaac tatcttttcc
     1201 cgaaacaagt aaagatgaag cggcgatgca gattgttgaa aggcttgccg tatatttgaa
     1261 caaataagca attgaacgga taaaccataa aacgggttgc ctgttaatca aaaggcaacc
     1321 cgttttacct gcttcaactt ctgatgactt tgcggatata tggaatacta tgcagatttt
     1381 gaataatctg attcaactga tccaaattct tgactttcaa taagaatttg aattcgacaa
     1441 aaccttccgt tcccgactgg gatttagacg gtgtttcgac cgactcgatg tccgcaccgg
     1501 aatcggaaat cgcttgcgcc attaatgcca acaggccgtg gctgtcttcc gattggactt
     1561 gaagcccgac acggtagttc tgcccgttca tattttccca gtctgcatcc agctgctgtt
     1621 cgggatcgga cttcaacaac gtcgggcagc tatccctatg gataatcatg ccttttccct
     1681 taaccaacag caaacggatg gaatcgccgg gaacagggtg gcagcactct gcaaaatgaa
     1741 tatgcccgct ttcctgacca tcgactttaa tggaactgag cctgacctcg ctgccgaaat
     1801 gctcccctgc caactcagca atgtgcatgg cgacataaac aggcagggta tgccccatcc
     1861 ctacgttgta cagcacttct tcaaacgatg tctgcttgtc gttgagatcg gcaagatatt
     1921 tttccttgat gccgtctgaa agcaggacat ctttgggcag caaactggac agggcttttt
     1981 gtaagaggct ctctcccaaa acgaccgcat cgtgccggtt aaggttttta atatattggc
     2041 gtatggcact gcgcgccctg ccggacacgg cgaaattcaa ccacgcggga ttgggtttgg
     2101 cgtgttcgga tgtgataatt tcaacagaat caccggtttt gagcttcgta cgcaacggca
     2161 tcatgatatt gttgatacgt gcggcaacgg ttttgtgccc gatatcggta tgcaccgcat
     2221 aagcaaaatc gacaggcgtt gcccctttgg gcaaagttag gatttttcct tttggcgtaa
     2281 ggatgtagat ttcgttcgga aacaaatcga ctttgacgtg ttcgagaaac tcaatggcat
     2341 tggcactgct tgcctgcaaa tctaagatat ttttcagcca ccggtttgtg tgaagcaccg
     2401 cctgatcgac cgtcttagaa tatgatttat agctccaatg tccggcgatt ccaccttcgg
     2461 caacagcatc catttccttg gtacgtatct gaacttcaat cggcaagccg taagggccga
     2521 ccaaagtcgt atgcagactt tgatacccgt tgcttttcgg aatggcgata tagtctttga
     2581 accgcccggg cttgggctga tagagggtgt gcaatgcgcc gagtgcggca taacaggctg
     2641 gaatgctgtt gacaatgacg cggaaaccgt aaatatccat aacctcggca aagcgcagct
     2701 ttttcgccat cattttctga tggatgccgt acaggttttt ttccctgcct ttgattttgg
     2761 cctctatatt cgcgcctacc agccgctggc cgaatgcgcg caagactttg ccgacaacgt
     2821 cctgccggtt cttccggctc ttgtccatcg ctttttttaa agtctcgtag cggttgggat
     2881 gcaggttttg gaacgataaa tcctgaagct cttgatatgc gttattcaaa cctatacggt
     2941 tggcaatctg tgcatagatt tcaagggttt cccttgcaat ccggcggcgt ttgtccgggc
     3001 gcatcgaacc gagtgtccgc atattatgca gacggtcggc aagtttaacg acaatcacgc
     3061 gcacatcttt ggtcattgcc aaaatcagtt tgcggaaact ctccgcctga tgttccgcat
     3121 gatcttcaaa tttgagtttt tcgagtttgg acagaccgtc caccatctcg gcaatcgtat
     3181 tgccgaacac cgccgccatt tccccttttg tcacgcccgt atcttccaat acgtcgtgca
     3241 tcacgcctgc acacagaccc tgtatgtcca tatgccaaag ggcgagctgc gtcgcaacgg
     3301 caattggatg cgtaatgtat ggttcgccac ttttccgggt ttgtccgtca tgagcgtgaa
     3361 atgcataggc aacggccttc tcgagctcaa cctgttcttc aggcttgaga taggaagcgg
     3421 tacggaaaag caaggtgcgt gcttcggcag ttaaatggtc gtaaggtgcg gtgggttgag
     3481 ggacaggcat ttcagacggc tttcggaaca tattttattt ttcgtttcaa accgtcggac
     3541 tgcacggcgg cagaatgtgc cgacgtgcgt ttccggcaga atttatttat tgcgcgtcaa
     3601 cagttccgta ccgatatgcc cggcggcgat ttcccttaag gcggtaacgg tcggtttgtt
     3661 attgcggaca tcgtccacaa gcggcgtatt gccattttca agctggcggg cccgacgggc
     3721 cgctaccaat gtcaggtcaa aatggttgga aatttttccg gtacagtctt cggtggtaat
     3781 acgtgccata ttatttgctt tctttcaaaa atatttaaaa taggaaactg ggtattttcg
     3841 cggtttttta ggaattttcc aacaaatctg caataaatcc cagttgccgc gaccttttca
     3901 gacggcaggc attcacaata tggcgcaaat cctcctccgc ccgcgccaag tcatcattga
     3961 ccacgacaaa atcgaacaat acggactgtt cgatttcatg ccttgccttc gacagcctcc
     4021 tttggataac ttcccgactg tccgtcccgc gtccgttcag acgtgcagca agcacatcga
     4081 aagacggcgg caggataaag atgccgacgg cttccggcag cgcgttgcga acctgcgccg
     4141 cgccctgaac gtcgatttcc aaaattacgt catagcctgc cgccgccaac gcattcacgc
     4201 cctccgcgct tgtgccgtaa tagttgccga acacgtctgc gtattccaaa aaagcttcct
     4261 gcgcgataag cgactcaaac tcttctttag aaacaaaatg ataatgtacg ccgtttactt
     4321 cgccttcacg cggcggacgc gtcgtgtgcg atacggaaac gcgcaaaccg ttatggtttg
     4381 ccaacagccg cgacaccagc gtggttttgc ccgtgccgga agcggccgaa atgataaaga
     4441 tgttgccttt tcgataagcg gacatatttt ttacctgtat attttccagc cgattgtatc
     4501 acaacggaca ctcggtttcc tatttgccga tgcccatatt ttgccgctat tgttttgatt
     4561 gatttggcaa gcggcaggct gacggctaca atatggcgtt aaaaacatca aacttggaac
     4621 acgcaatgct ggttcatccc gaagctatga gtgtcggcgc gcttgccgac aaaatccgca
     4681 aaatcgaaaa ctggccgcaa aaaggcatct tattccacga catcacgccc gtcctgcaaa
     4741 gcgcggaata cttccgactt ttggttgatt tattggttta ccgctatatg gatcagaaaa
     4801 tcgacatcgt tgccggtttg gacgcgcgcg gcttcattat cggcgcggca ctcgcctacc
     4861 atctcaacgt cggttttgtc cccatccgca aaaaaggcaa gctgcctttt gaaaccgtat
     4921 cgcaaagcta cgcgctcgaa tacggggaag ctgcggtgga aatccacacc gatgccgtca
     4981 aacccggttc gcgcgtgctg ctggtcgatg atttggttgc cacgggcggc acaatgcttg
     5041 ccgggctgga actgatccgc aaactcggcg gagaaattgt cgaagctgcc gctattttgg
     5101 aatttaccga ccttcaaggc ggcaagaata tccgtgcaag cggcgcgccc ttatttaccc
     5161 tgcttcaaaa cgaaggctgt atgaagggct gaaaaccgac cctgccgtct gaaaccggca
     5221 gggttgttat tatgcgttca aatcacgccc aaatcttgca agcccctcaa cacgccgtct
     5281 tcatcaacgc tggggcaaac atatttcgcc gcttctttcg ccgcctgttc cccgttgccc
     5341 attgccacgc cgaacccgac ttcagacagc atttccacat cgttcaaacc atcgccgaac
     5401 gccatcacgt ccgccatttc caaacccaat gcttcaacca cgcttctgat gccgtctgtt
     5461 ttcgacgcac ccgcaggcag cagatcgacc gcttcctcat gccagcgcac cgttttcaag
     5521 ccttcccgtt ccacaatatc cgaccaaagc ggcatttcgt tttcctccgc aaacaccagc
     5581 atctgataca ccggtttgct tgaaaaataa tccttatcgg caaaaaaatc gctggcgata
     5641 tgcttcaagg cgcggcacac gcattccgac agtgcggaca ccgcaatccc ctctccgccg
     5701 acaaacgcat aatccatgcc caagccatcc aaatgcgcgc aaaccctgcc catcaaaccg
     5761 gcatccatcg gtacttcgtg cacagttttt ccgtgcagca gcgcaaactg tccgtttatc
     5821 gttaccacgg catccatacc cgtttccgcc atcatgtccc tgaccttttc gggaatcgtc
     5881 gccaaagacc gccccgttgc caacgccgtc aatatacctt tgccgcgcaa agccgccacc
     5941 gccgttttca cggaaggacg caaggtatcc gtatatttgc ggtacagcgt atcgtcaata
     6001 tcgaaaaaca cgattttggg attcatcaca ttctctctcg cattcaaatt gccgcattgt
     6061 atcccaagca ggcaaatact tgataaatcc ttataaattt cccggcaaaa ttgaccgcga
     6121 acacgaaaag gcggataatc cgcccatcct caaacccttt tcagacggca tttgcagcaa
     6181 tgccgtctga aacattttta caaagcacac aaatcatgtt tcaacacaca ggacgacaca
     6241 taaagcgtcg ccctatatgt tgccctgatt cggaaagggt tacacccctc ccaaataaag
     6301 tctgattcta ccgccctaaa gggcggggtt tcaaccgaaa aggaaacacg atgaaagcac
     6361 ccgaactctt attgcccgcc ggcggattgg aaagaatgcg cgccgcctac gattacggcg
     6421 cagatgccgt ttacgccggc agcccgcgtt actccctgcg cgcccgcaac aacgaatttg
     6481 ccaaactcaa cgtccttgag caaggcatta aagaagcgca cgagcgcaac aaaaaattct
     6541 ttttgaccgt caacaccctg ccgcacaact ccaaactcaa aacctttgtt tccgatatgg
     6601 aacccttgat tgccatgaaa cccgacgcgc tgattatggc ggatccgggt ttgatcatga
     6661 ccgtgcgcga aaaatggccg gaaatgccga tccacctgtc cgtacaagcc aacaccacca
     6721 actactgggg cgtgaaattc tggcagaata tcggcgtcga acgcatcatc ctgtcgcgcg
     6781 aattgagtat ggaagaaatc gccgaaatcc gccaacaatg ccccgacatc gaactcgaag
     6841 tcttcattca cggcgcattg tgcatcgcct actccggccg ttgcctactc tccggctact
     6901 tcaaccaccg cgaccccaac caaggcacct gcaccaactc ctgccgttgg gactacaaag
     6961 tccacaacgc tacggaaagc gatgcgggcg atgcccggct tctgcaaggt ttcaactttg
     7021 aaaaagccca agaagaagcc aaccaaaact ttgaaggcat caacggtcaa aaacgccatc
     7081 cctacgccga caaggttttc ctgattgaag aatccaaccg tccgggcgag atgatgccga
     7141 ttatggaaga cgaacacggc acctacatca tgaactccaa agaccttcgt ggcattgaag
     7201 tggttgagaa actcgccaaa atcggcgtgg acagcctcaa agtcgaaggc cgcaccaagt
     7261 cgctctatta tgtcgcacgc gtcgcccaat cctaccgcaa agcgattgac gatgccgtcg
     7321 caggccgtcc gtttgattac agcctgttga gcgaactcga aggccttgcc aaccgcggct
     7381 acaccagcgg cttcctcgaa cgccaccaca ctcaggatta tcaaaactac ctgaccggcc
     7441 attccaccgc caaacaaagc caatacgtcg gacacgttac cgaaatcgac aaaaacggct
     7501 gggcgaccat cgaagttaaa aaccgctttg ccgtcggcga ttcgctcgaa atcatccacc
     7561 cgaacggcaa ccaaaccatc aaattggaac aaatgacccg caaaggccaa cctgtcgatg
     7621 tcgcacccgg caacggcatc caggtcaaaa tccccaatat gcggggcaaa gaaaaagccc
     7681 tcatcgcacg cgtgttgaac ccctgagcca caatgccgtc cgaaccccgt tttcagacgg
     7741 cattttgttg aatcttacgg tacaatggca atgatttccc acagcaaaca aggacatccg
     7801 acatgaaaaa aaatcctcat actctccgcc gccgccctga tgccggcagt ctgccaatcc
     7861 ggcagcgcac ctaaaaacgc gcacacggca tccgccggca caccggccgg agaatcatgc
     7921 aaagtcatcc gggacgacac cgcaggaagc ggcaaaaaca tcatttaccg ttgcagcaac
     7981 ggtcaggcgg caataaatgc agccattgca gacggcgtat tggaatcagg gattcccgtc
     8041 agcttcggcg gcgggggcgt accggttgca aacggcagga atctggttac acggcaagca
     8101 gcaaacggcg ttggcaaatc cgactccgag gcctgcgaac acgccctaat caatgccgcc
     8161 cgcaaattcc agcagaccgc aggcaaactc ggcgggcgca gtgttaccgg cttccacagc
     8221 tacttcgaca agcagccgct gcaaggcggg caatacgact gtcagacagg ttcgttccac
     8281 gtccgcgtgg tcatgcgcgc caatgtcgtc cgttaacata tcggcaatca aaaaattagg
     8341 ctgtgtttta gtaatctgtt gatttcaatt atttgcaaag gaaaagacaa ttattttttc
     8401 ggttaggaag agacctatcc tattgaatac atttaagtta aatacgtcta tcaacactat
     8461 attatagtgg attaacaaaa atcaggacaa ggcgacgaag
//
LOCUS       DAWFAD010000083         8390 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWFAD010000083 DAWFAD010000000
VERSION     DAWFAD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.083"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            37..174
                     /locus_tag="WGO49_001371"
     CDS             37..174
                     /locus_tag="WGO49_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-like fold family protein"
                     /protein_id="HEZ0306476.1"
                     /translation="MHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHLEKVF
                     G"
     gene            complement(383..1768)
                     /gene="pntB"
                     /locus_tag="WGO49_001372"
     CDS             complement(383..1768)
                     /gene="pntB"
                     /locus_tag="WGO49_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ0306477.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKGANSVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1834..2217)
                     /locus_tag="WGO49_001373"
     CDS             complement(1834..2217)
                     /locus_tag="WGO49_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ0306478.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAAQSTINTLACLFILFRLAFIWCYIA
                     DKAAMRSLMWAGGFACTVGLFVAAA"
     gene            complement(2221..3762)
                     /locus_tag="WGO49_001374"
     CDS             complement(2221..3762)
                     /locus_tag="WGO49_001374"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ0306479.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWACPLIYKVNAPSEGELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTHDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVSLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLN
                     MFKKG"
     gene            4053..4886
                     /gene="serB"
                     /locus_tag="WGO49_001375"
     CDS             4053..4886
                     /gene="serB"
                     /locus_tag="WGO49_001375"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ0306480.1"
                     /translation="MPQALVLQFPSAEALPSDFPSRLPEPDCADEKRMRFIVEEGFSL
                     SGADAALLGSRQIDHAVLPDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKAQAAADACINFGGLERVRGLF
                     G"
     gene            4950..6172
                     /locus_tag="WGO49_001376"
                     /pseudo
     CDS             4950..6172
                     /locus_tag="WGO49_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            6337..7917
                     /gene="purH"
                     /locus_tag="WGO49_001377"
     CDS             6337..7917
                     /gene="purH"
                     /locus_tag="WGO49_001377"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ0306481.1"
                     /translation="MPSIKRALISLSDKTGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLDEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     LNRADLKVVSKRQPTEQEWNDLMFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDANLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         1875 a         2287 c         2379 g         1849 t
ORIGIN      
        1 gatttctttg aaggcaaagg cgttgatgtt ttgtatatgc acttccacaa agccaacgag
       61 tttttgggta tgacccgcct gccgacattc ttatgtaacg atgtggttaa aaatccgcaa
      121 gtggaaaaat acttggcaga ttatcaggca cacttggaaa aagtgttcgg ctaattaaaa
      181 atccatcttc aacacggaga tggattttgt ttgtttcgtt gattttgtgt cagtttcaga
      241 tgtaggtgct tattcggacg ggccgtctga aaatgtttgc cccaatgcaa aaaaaatcac
      301 tgcaaacctt cataaacggg gtttgcagtg attttttcaa atcaaacaga ttgaaaacct
      361 gcgccgaatt gttcagacgg cattattttt tcagttcgga cagaatgtca tccacggttt
      421 tcttcgcatc tccgaaacac attacgctgt tttcgttgaa gaacagcggg ttttgtacac
      481 ctgcgtagcc ggtattcatc gagcgtttga agacgacgac ttcttttgcc ttccatactt
      541 ccaacacggg catacccgca atcgggctgt tcgggtcggt ttgggcggca ggattgacgg
      601 tatcgttcgc accaatcacc aagaccacat cggtttcggg gaagtcgtcg ttgatttcgt
      661 ccatttccaa cacgatgtcg taggggactt tggcttcggc aagcagtacg ttcatatgac
      721 cgggcaggcg gccggcgacg gggtggatgc cgaagcgcac ttctgtgccg tttttacgca
      781 aaagctcggt gatttcggca acggggtatt gcgcttgtgc gactgccata ccgtagcccg
      841 gggtaatgat gacactgttt gcgcctttca gcatttcggc aacatcggca gctttgactt
      901 ctcggtattc ccctatctct tggctgccgg aagatgatgt gccgctgtcg ctgccgaaac
      961 cgccggcgat taccgagaca aacgaccggt tcatggcttt gcacataatg taggacagaa
     1021 tcgcgccgct tgagccgacc aacgcgccgg taacgatgag caggtcgttg gagagcataa
     1081 agcctgccgc tgcggccgcc cagccggagt aggagttgag catggacacg accacgggca
     1141 tatctgcgcc gccgatggag gcaaccaagt gccagccgaa tgcgagggca atcagggtca
     1201 taatcagcag gatgaagccg ctgccgtcaa tgccgacgaa tacgagcagc aacacaaacg
     1261 atacgccaag tgccagtgcg ttgagcttgt gtttggcggg cagttgcagc gggctgctgc
     1321 tgattttgcc gttgagtttg ccgaatgcga ccagcgagcc ggtaaaggtt accgcgccga
     1381 tgaagatgcc taaatacact tcgaccagat ggatggtgtg catatcgtgc gaaacgttgc
     1441 ccggcgcgat atagctgttg aagccgacca aaaccgccgc taggccgacg aagctgtgca
     1501 gcagggcaat cagttcgggc atttcggtca tttccacctt tttggctttg tagatgccga
     1561 ttgccgcgcc gatgagcatg gcgatgatga tccagcccag tccgtgggta ttgtcggaaa
     1621 aaacagttac aaaaagggcg accgccatac cggcgatacc ggaatagcag ccctgtttgg
     1681 cggtttcctg tttggacagc cccgccagtg agaagatgaa taaaattgcg gcaacgatat
     1741 acgccgctgt tacgagtcct gaagacatgg aaattctccg attttcgatg atttgttttc
     1801 aatgccgtcc gaactgttca gacggtatct gtttcaagca gccgcgacaa acagcccgac
     1861 ggtgcaggca aatccgcctg cccacatcag tgagcgcata gcggctttgt cggcgatata
     1921 gcaccagata aaggcgaggc ggaacaggat gaacaggcag gcaagcgtgt tgatggtcga
     1981 ttgcgccgca ttgccggttg cgtgtgccgt caaaacggcg gcggcaaacg gtgcaaaggc
     2041 ttcaaaaccg ttttgctgcg cggcgtgggc acgggcggct gtgccttgcg tgtgcgccag
     2101 aaaatcgcgc ggattgtggt tgtctttaaa ccggaatccg cccgcttttt tggcatatgc
     2161 cgcacaaaaa agcggcaata ggcaggcaat caggatgcac caataggcga aagtcatggc
     2221 ttaccctttc ttaaacatat tcagcatacg ccgtgtaacc gcaaagccgc cgaagatgtt
     2281 gatgccggca atcaggatgg caacaaacga cagcagcgaa acaaagccgt tgccctgacc
     2341 gatttgcagc agcgcgccga cgacgatgat gccggagatg gcgttggtca cggacatcag
     2401 cggcgtatgc agcgagtggc tgacattcca aacgacgtag tacccgatga cgcaggcaag
     2461 gacgaacacg ataaagtggt tcaggaatgc tgcgggtgcg accgcgccga cccacagcac
     2521 caatacggcg gcgatgacgg cgggcgcgag ttttttccac agggaaacag gtttcggctc
     2581 gggcttggcg gcaggcgcgg ctttttcaga cggcgtttgc tgcggctggg cggaaacttg
     2641 aatcggcgga ggcgggaagg tgatttcgcc gtcgtgggta acggtcatgt tgcggataat
     2701 cacgtcttcg aagtccaacg tgatttcgcc gtctttgttc gggcttaaca gcttggtcag
     2761 gttgaccaag ttggtggcgt aaagctggga agactgtccg gcaaggcggt ttgccatgtc
     2821 ggtgtagccg atgattttca cgccgttgcc ggttacggac aattcgcccg ggcgggtgag
     2881 ttcgcagttg ccgcccgtcg ccgccgccaa atcgacgatg acggagccgg atttcatgct
     2941 ttccaccatt tctttggtaa tcagcttggg cgcgggtttg cccggaatgg cggcggtggt
     3001 gatgatgatg tccacttctt tcgcctgctc ggcaaagagc ttcatctcgg cggcgataaa
     3061 ttcgtcgctc atcactttgg cgtagccgtc tccgctgccg cccgattctt gtgggaagtc
     3121 gagtttcagg aacttgccgc ccatcgattc gatttgttcc gccacttcca agcgggtatc
     3181 gaacgcgcgt accactgcgc ctagtgaatt tgccgtaccg attgccgcca aacctgccac
     3241 acctgcacca atcaccaaaa cctgcgcagg cggcactttg ccggcggcag taatttgacc
     3301 ggtgaagaaa cggccgaagg cgttggcggc ttcaatcacg gcgcggtagc cgctgatgtt
     3361 tgccatcgaa gacaaagcgt ccaaagcctg cgcgcgggaa atacggggca ccatgtccat
     3421 cgccagcgca ttgactttct tggcgcgcaa ggcttcgacc aaagcctcgt tttggcgcgg
     3481 ccacaggaag ctgacgatgg tttgaccttc gttcaaaagc ggcagctcgc cttcggacgg
     3541 cgcgttaacc ttataaatca aagggcaggc ccaaaccgcc gctttgtcgg caacggttgc
     3601 gcctgctgtt tggtaagcgg catcgtccaa acttgccgcc aaacctgcac cgctttcgac
     3661 aacggtttca aagcccagtt tgcccagcag ggcgacggtg gcgggcgtgc aggcgacgcg
     3721 ggtttcgccg gataatgact cgcgtgggat accgattttc atctctgaat cctttttcgg
     3781 gtagtttata tgtatcgtgg gttaaattta aatcgggggg ggggcggagc aacgccgtac
     3841 cggtttaaag ccgactcact tcaaatgtta atatatttta gataatcccc ttataacgaa
     3901 ttttcatcag gctggcaata gttgcggcat tttcccgtgt tgtccgacac atactgccgt
     3961 ttgtgtttga agtcattttc cgattgcagg cccgcccgcc gtaacattct ttgccgtttc
     4021 actatataat ccgcattttt tgagccgccg ttatgccgca agccctcgtc ctccaatttc
     4081 cctccgccga agccctgcct tccgacttcc cctcccgcct gcccgaacct gattgcgccg
     4141 atgaaaagcg tatgcgtttt atcgttgaag aagggttttc tttaagcggg gcggacgcgg
     4201 cgttgcttgg cagccgtcaa atcgaccacg ccgtgttgcc ggatatggat ttcggcgaac
     4261 tcggtttgat tgtcagcgat atggattcga cgctgattac catcgaatgc gtcgatgaaa
     4321 ttgcggcagg cgtgggttta aaagacaagg tagcggaaat taccgagcgt tcgatgcgcg
     4381 gagagttgga tttcgagcag tcgttgcgca gccgcgttgc gctgttggcg ggattggacg
     4441 aacagatttt ggcggacgtt tatgaaaacg ttttgaagct ctcgcccggt gcggaatttt
     4501 tgttggacga atgcaaaagg cacgatgtga aattcctgct ggtgtccggc ggcttcacgt
     4561 tttttaccga acgtctgcaa caacgcctcg gcttcgaata ccaacacgcc aatgttttgg
     4621 aaattgaaaa cggcaggttg accggccgtc tgaaaggcag aatcatcgac gcgcaggcaa
     4681 aggcggattt attgcgcgaa taccgcagcc gcctcggatt gcagccacat caggtgttgg
     4741 cgatgggcga cggtgcgaac gatattccga tgctcaaaga agcgggcata ggcgtggctt
     4801 accgtgccaa accgaaagcg caggcagccg ccgatgcctg tatcaatttc ggcggtttgg
     4861 agcgtgtacg cggcctgttc ggataggcgg ataggaaacg gatgtcgtcc gaaaggtttt
     4921 cagacggcat ttgaacggca ggaacgacag tgggacgcag aaaactttgg tttgccctgg
     4981 cagcagcagg cgttatcggc ggtttggtcg gcattgtgct gacggaactg atgcacttca
     5041 tacagcatac ggcaacggtt atggcgcgga cggcgtgtac acttcgttcc gcgaaggcgt
     5101 ggcacaggct tccggtatgc ggcgcgttgc cgtgctgacg ctgtgcggcg cggtcgcagg
     5161 cagcggctgg tggttgctga aacgtttcgg caagccgcaa atcgaaatca aagccgcctt
     5221 gaaacagccg ttgcaggggc tgccgtttct gacgacggtt ttccatgttc tgctgcaaat
     5281 cataacggtc ggactcggtt cgccgctcgg acgcgaagtc gcgccgcgag aaatgaccgc
     5341 cgcgtttgct tcggcaggcg gcaaacgctt gggtttggac gaaggcgaaa tgcgcctctt
     5401 gattgcctgt gcgtccggcg cgggtttggc ggcggtgtat aacgtgccgc tcgcctccac
     5461 gctgtttatc ctcgaagcga tgttgggcgt gtggacgcag caggccgccg ccgccgcgtt
     5521 gctgacttcg gtcattgcca ccgccgtcgc gcgcatcggc ttaggcgacg tgcagcaata
     5581 tcatcccgcc aaccttgccg taaacacctc attactttgg ttttccgccg tcatcggccc
     5641 gatactgggc gcaacggcgg tatatttccg gcgcagtgcc gaaaagttcc ccttcctcaa
     5701 gcgcgacaat ccgagaatta ttcctttggc gattgctttg tttgcactca tcggcgcgat
     5761 tgccgtttgg tttcccgaaa ttttgggcaa cggcaaagca ggcaaccaac tgacctttgg
     5821 cggattgacc gactggcaac acagccttga gctgaccgcc gtcaaatggc tggttgtctt
     5881 gatggcgttg gcggcaggcg catacggcgg actgattacg ccgtctatga tgctcggcag
     5941 caccatttct tttgcagccg ccgccgcgtg gaacagcgtt ttccccgaaa tgccgtctga
     6001 aagcgcggca gtcgtcggcg ccgccgtttt cctcggcgtt tccctcaata tgccgctgac
     6061 cgccgccgtc tttattttgg aactcaccta cgcccccgcc gctttgttga tgcctttatg
     6121 tacgggtatg gcgggcgcgg tatggacggc aaggaaaatg ggatgtaaat aaccgaaagg
     6181 caaaatgccg tctgaaaccg gttttcagac ggcattcctt tggggattgt caacaatatc
     6241 tgcaaaaacc tactgccgga aagtggaaaa tagcggataa taccgccccg acaacattac
     6301 cgtttcccat cccaacgtca gaaaaaggaa atccctatgc cttccatcaa acgcgccctg
     6361 atcagcctgt ccgacaagac gggcgcagtc gaatttgccc aaaccctgac caagctcggt
     6421 gtcgaaatcc tctctaccgg cggcacggcg aagctgcttg ccgatgcggg cgttcccgtg
     6481 attgaagttg ccgactacac cggtttcccc gaaatgctcg acggccgcgt gaaaaccctg
     6541 catccgaaaa tccacggcgg tattttgggc cggcgcgatt tggacgaaca cgtcgccaag
     6601 atggaagaac acggcatcgg caacatcgac ctcgtgtgtg tcaacctcta ccccttcgcc
     6661 gccaccatcg ccaaaccaaa ctgcacgctg gaagacgcga ttgaaaacat cgacatcggc
     6721 ggcccgacca tggtgcgctc cgccgcgaaa aactggaaac acgtcgccat cgttaccgac
     6781 acagccgatt tcccggccat cgccgccgaa ctcgaagcca acaatggcgc attgagcgac
     6841 aaaacccgct tcaacctgtc gcgcaaggcg ttcagccaca ccgcccaata cgacggcatg
     6901 atttccaact acctgacctc actttcagac gacgttttga gcggagagcc tgaaatcggc
     6961 gaatttccaa gccagttcaa ccaaagctgg attaaagtgc aagacatgcg ctatggcgaa
     7021 aacccgcatc agcgcgccgc gttctaccgc gatattgacc ccgccgcagg cagcctttcc
     7081 gcctacaaac aactgcaagg caaagaattg tcttacaaca acatcgccga tgccgatgcc
     7141 gcatgggaag ccgtcaaatc ctttgacgcg ccagcctgcg tgatcgtcaa acacgccaat
     7201 ccgtgcggcg tcgccgttgc agccgataca ttgaccgcct acaaactcgc ctacgtcacc
     7261 gacaccacca gcgcgttcgg cggcatcatc gccttcaacc gcgaagtgga cggcgaaacc
     7321 gtcaaacaga ttaccgacaa ccagtttatg gaagtcttga tggcgccgaa gtttaccgcc
     7381 gaagccctcg aaatcgccgc cgccaagaaa aacgtgcgcg tattggaagt gccgcttgag
     7441 gcaggcgcaa accgcttcga actcaaacgc gtcggcggcg gactgttggt acaaacccct
     7501 gatattcacc gcctcaaccg cgccgatttg aaagtcgtct ccaaacgcca accaaccgag
     7561 caagaatgga acgacctgat gtttgtttgg aacgtcgcaa aatacgtcaa atccaacgcc
     7621 atcgtcttcg gcaaaggcgg tcaaacctac ggcatcggcg caggccaaat gagccgcgtg
     7681 gacagcaccc gcatcgccgc ccgcaaagcg caagatgcca atctcgacct caacggcgca
     7741 tgtgccgctt ccgatgcttt cttccccttc cgcgacggcg tggacgtgat tgccgagcag
     7801 ggcatcaaag ccatcatcca tccggcaggt tcgatgcgcg atcaggaagt cttcgacgca
     7861 gcggacgaac acggcatcgc catggtcgta accgacgtgc gccacttccg ccattaacgc
     7921 agatgaacaa ggcaatgccg tctgaagggc tttcagacgg cattttgcgc tattttgtga
     7981 aggtagggat gacggttcgg gtattcctga cagggtggat tttcaaggtg ttgtataggg
     8041 tgtaggagga ttcgtaaaag gtgggatgca gggtgtgctt cagcccgctg catcaaaaat
     8101 ttttggagaa tcggcgggag tcggcggttt tggtttcggc ggggacggtg gaaatgggta
     8161 acattgacgg aatcgacgga agcggtgggc ttcagcccac cgcttgtata ttggaatcac
     8221 cgtatcatag cgccacccgc gcccacccaa ggcacacaac cgttgcgtag ctcagggagc
     8281 ggcagggcaa cccatcgaca caaccggaca gttgccggac aacacaaccg aatgcaaggc
     8341 aggtttatga tgagtaccca ataccattac gcaggtatag tgaattaaat
//
LOCUS       DAWFAD010000084         8247 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWFAD010000084 DAWFAD010000000
VERSION     DAWFAD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.084"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(149..1081)
                     /gene="cysK"
                     /locus_tag="WGO49_001378"
     CDS             complement(149..1081)
                     /gene="cysK"
                     /locus_tag="WGO49_001378"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ0306482.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1275..1880)
                     /locus_tag="WGO49_001379"
     CDS             complement(1275..1880)
                     /locus_tag="WGO49_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306483.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="WGO49_001380"
     CDS             complement(1877..2728)
                     /gene="dapF"
                     /locus_tag="WGO49_001380"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ0306484.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPRFMPSEIPFVPELGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAEAVF
                     EGELAYS"
     gene            2865..3161
                     /locus_tag="WGO49_001381"
     CDS             2865..3161
                     /locus_tag="WGO49_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306485.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANRILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3175..3843
                     /locus_tag="WGO49_001382"
     CDS             3175..3843
                     /locus_tag="WGO49_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0306486.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3915..6035)
                     /gene="pnp"
                     /locus_tag="WGO49_001383"
     CDS             complement(3915..6035)
                     /gene="pnp"
                     /locus_tag="WGO49_001383"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ0306487.1"
                     /translation="MFNKHVKTFQYGNQTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYVNGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQVAINA
                     INEFADEVNPELWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTSV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RMGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6272..7135)
                     /locus_tag="WGO49_001384"
     CDS             complement(6272..7135)
                     /locus_tag="WGO49_001384"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ0306488.1"
                     /translation="MSEIGLVKIYFGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLVHIMPNHFTGQTVYDVLPENEAKALEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIQLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECERIIGKELAAEVRAKAIQLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPANVIQKTVEK
                     YQEALTLLTQD"
     gene            7334..8197
                     /gene="rfbD"
                     /locus_tag="WGO49_001385"
     CDS             7334..8197
                     /gene="rfbD"
                     /locus_tag="WGO49_001385"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ0306489.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGNAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLL"
BASE COUNT         1975 a         2377 c         1910 g         1985 t
ORIGIN      
        1 atatttccct caagcgatgt ggcggcacca acacctgatt aaaacacaac cggcgttttc
       61 ggcatatacg caaaaacgca ttttccggca cggcgttaca ccgataccga aaaatgcgtc
      121 tgaatgtttc gacaatccga ttaaagcatt atgccaaatc tgcaaaaagt ggcgtagaga
      181 gatagcgttc gccataagaa ggcagcagca cgactatcag cttgccttcg ttttcaggct
      241 gtttggcaag ctgcaacgca ctccaaaccg ccgcaccgga agaaataccc accaaaatgc
      301 cttctttttc cgctattgcg cgggcggttt caaaagccgc ttcgttcgac actttggtaa
      361 tgctgtcgta aattttggta ttcaaaacgg tcggaataaa gcctgcgccg atgccttgaa
      421 tcgggtgcgg gcctttttcg ccgccgctca atacgggtga agcttcaggc tcgacggcaa
      481 ccactttaac ttcgggtttg tattttttca acacttcgcc cacgccggta atcgtaccgc
      541 ccgtgccgac gccggcaacg aagacatcga ctttgccgtc cgtatcccgc caaatttcct
      601 cggcggttgt tttgcggtgg acttcggggt ttgcctcatt gtcgaactgg cgcggcataa
      661 aataagtgtc gggatgcgcg tccaccaagg atttcgcttt ggcaatcgcg cccgccatac
      721 cttcggcggc aggggtcaga atcagctccg caccaaacgc gcgcaacagc attttgcgct
      781 ccttactcat gctttccggc atggtaatcg ccagcttgta gccgcgtgcg gcacatacca
      841 ttgccaaacc gacacccgta ttgccgctgg ttgcttcgac aatgacggtg tttttgttga
      901 ttttgcccgc tttttcggca ccctcgatca ttgcttcggc aatgcggtct ttgacgctgc
      961 tgcctggatt gaaaaattcc agtttcacgg caacctccgc cttcaaacct tcggtcagac
     1021 ggttcagttt gaccaaaggc gtgttgccga ttagttcggt gatgctgttt gcaattttca
     1081 tcttctgctc ctgaataata aggtttcgac agtaaggcgc aatccccata ggcatcctgt
     1141 ccgattgcag gggatttgcg ccgaaacata aacaaatttc ggatatggct ttatcctaaa
     1201 cctatgccgc ctaaaaaacc aatatccaaa atttctttct ttataacttt ttgttccgat
     1261 acggccgcac cgtattatct tccaatttct gaaagcagca gcaccagtac cgagacctgc
     1321 atcaacgctg ccgtcaaccc gccgagtcgc agctgcgagc cgccgaacgt tgacggcggc
     1381 tgccggtcta taccgcagcg gtggacatag gcaaccgcca tcagcacaaa ctgcaacacg
     1441 aaccaataaa ccggcttcag cgcataaagt ttgagcagtg ccgcaaacac aaacacttga
     1501 accgttcccg aaaagcaata atgcaccaac gcacatattc cgacaaaagc aagcgtcatc
     1561 gatacattac tgacggcaaa cagcccgagc agcggatgtt cggggtctgc ctgccatccg
     1621 tttccatccg ttttccggtt ccaatgcccg atgaaaaaaa atatgctgcc caaagtcagg
     1681 taaaaatggg ggatgaacaa gggcgcggcg cgggtcattc cccatatgcc gggcatcagc
     1741 tttgccccca aaaccgatat gcccagccac agcataatac tcgcccaaaa ccattgcaac
     1801 attcctgcgc ggaatatcag aaacagaaaa accgccgcat acagtaaggc agacaaaaaa
     1861 tccaaatgca gcaaaatcat gaatacgcca actcgccttc aaacaccgct tccgcagggc
     1921 cggtcatcat cacatcgccg ccgcaggccc attcgatata taaagtcccg cccggcaaaa
     1981 tcacctctac cgttttccct tcatccaaca accccagacg gatacccgcc accacagccg
     2041 cacacgcgcc cgtaccgcac gcttgggttt cgcccacgcc gcgctcgaac acgcgcaaac
     2101 gaatcgcggt tcggctgaca atctgcataa agccgacgtt gacgcgttcg ggaaactgcc
     2161 tgtgcggttc gataagcgaa ccggtttcgc gcaccggcgc gcattctacg tcatcgacca
     2221 caatcaccgc atgggggttg cccatattga cgcagctgac aggctgaatg ccggattcga
     2281 gatgcacccc gtaaatacag gcatcatccc cctcgcccaa ttcggggaca aacggtattt
     2341 cagacggcat aaacctcggt ttgcccatat tgaccgtaac catgccgtta tcggacaatt
     2401 tcggaaaaat aacgccattt gccgtttcaa cacaaatttc tttcttatcg gtcaaaccct
     2461 tgtctgcaac aaaacgggca aaacaacgcg ctccgttgcc gcattgcccg acctcgctgc
     2521 cgtcggcatt gaaaatacgg taacggaaat ccacgccttc ggtttccgaa cgcccgacca
     2581 ccaaaagctg gtcgaagccc acgcctcgga agcggtccgc ccatgccgca atcggcgcgt
     2641 cctcgggggt aaaatcctga ctgaccgcgt caatcaccat aaaatcgttg cccaaaccgt
     2701 gcattttggt aaatttcaac gtgttcatat tgttccgaaa tcccgtatcg tctgttgctt
     2761 ccgaatccga aatcccccgc accgaaggga aatgcttcca tctcccgccg cctgaggatt
     2821 aaaatcagcc attatactgt ttgttacccc ttacaagaaa caccatgttc cgcatattac
     2881 tgttgctttt cccttgtgca atccttgccg cctgcacccg aaacataccg gcaaacccgg
     2941 ccgtttcaaa ttgcgcgcat cttcaaaacc gcaccatcct gtattttgac gcacacggca
     3001 aacccgccga cggcgcagta aagggcggat tttacaaaat tatcgaaata cagggtgcaa
     3061 accgcatcct tattcaagac tttaacagca cgggcaatgc cctcggcgga tcctatgtgc
     3121 cggactgccg caaatcatcc cttttcccga aaaatatttg acttcaaact gcctatgaac
     3181 cacgacatca ctttcctcac cctgttccta ctcggtttct tcggcggaac gcactgcatc
     3241 ggtatgtgcg gcggattaag cagcgcgttt gcgctccaac tccccccgca tatcaaccgc
     3301 ttttggctga tcctgctgct taacacagga cgggtaagca gctatacggc aatcggcctg
     3361 atactcggat taatcggaca ggtcggcgtt tcactcgacc aaacccgcgt cctgcagaat
     3421 attttataca cagccgccaa cctcctgctg ctctttttag gcttatactt gagcggtatt
     3481 tcttccttgg cggcaaaaat cgagaaaatc ggcaaaccga tatggcggaa cctgaacccg
     3541 atactcaacc ggctgttacc cataaaatcc atacccgcct gccttgcggt cggaatatta
     3601 tggggctggc tgccgtgcgg actggtttac agcgcgtcgc tttacgcgct gggaagcggt
     3661 agtgcggcaa cgggcgggtt atatatgctt gcctttgcac tgggtacgct gcccaatctt
     3721 ttggcaatcg gcattttttc cctgcaactg aaaaaaatca tgcaaaaccg atatatccgc
     3781 ctgtgtacgg gattatccgt atcattatgg gcattatgga aacttgccgt cctgtggctg
     3841 taagccccat tcattcccga tacccgtaaa aataccgtct gaaacctgtt cagacggcac
     3901 tttcacccta agcgttactc ggcggcattt tcctcacggg caggcgcgtc cagcagggct
     3961 ttgatggaca gacggacacg gcctctgtcg tccacttcca atgctttcac gttcaccacc
     4021 tgaccgactt gcaggtagtc gccgacattg cgtacgcgct cgtgggcgat ttggctgatg
     4081 tgtaccaaac cgtctttgcc cggcatcacg ctgacaatcg cgccgacatt gttgtcgagg
     4141 attttcacca cagtgccttc gtacactttg cccacttcca cttcggcagt aatctgctcg
     4201 atgcgttttt tcgccgcatc gccggcttct tgagtggttg cggcaatggt aatcgtaccg
     4261 tcttcggcaa tattgatttc cgtaccggtt tcagcggtaa tcgaacggat ggtttcaccg
     4321 cccttaccga taacttcgcg gattttgtct tggttgattt tcatcgtgaa caagcgtggc
     4381 gcgtgtgcgg acagctcttg cgggcccgca acggcggctt tcatctgatc caagatgtgc
     4441 agacgcgctt ctttggcctg tgccaaagcg atttgcataa tttctttggt aatgccttgg
     4501 attttgatgt ccatttgcag cgcggtaacg ccttcggtcg taccggccac tttaaagtcc
     4561 atatcgccca agtggtcttc gtcgcccaaa atgtcggtca ggacggcaaa tttgttgcct
     4621 tccagaatca gacccatcgc gataccggca acgtgtgctt tcaaaggcac gccggcagac
     4681 agcaggctca ggcagccgcc gcagacggaa gccatagagg aagagccgtt ggattcggta
     4741 atttcggaga ccacgcgcat ggtgtagctg aaatcttcag gtttcggcaa tacggccaac
     4801 aatgcacgtt tagccaaacg gccgtgaccg atttcacggc gtttcggtgc gcccatgcgg
     4861 cccacttcgc cggtagagta cggcggaaag ttgtagtgca gcataaagcg gtcggtgtat
     4921 tcgccggaca gcgcgtcgat gatttgctcg tcgcgcgaag tacccaaagt tgcaacggcc
     4981 aaagcttggg tttcgccacg ggtaaacaat gcagaaccgt gcgtgcgcgg caatacgctg
     5041 gtttggatgt tcagcggacg gacggtgcgg gtgtcgcggc cgtcgatgcg cggttggcca
     5101 tccaaaattt ggctgcggac gacatcggct tccaagtgtt tgaaaatgcc tttgatttcg
     5161 ttggctgcca aagtgtcggt ttcttcggta atcaacgctt ctttcacggc attccaagct
     5221 tcgtccaact tggcagaacg cgcttgtttt tgacggattt tgaacgcttc tttaatggtt
     5281 tcgccggcaa tcccgcggac tttggcaacc agttcctcat tggtttcagg tgctttccaa
     5341 tcccaaagtt ccggattgac ttcgtcggca aattcattga ttgcattgat ggcaacctgc
     5401 atttgatcgt ggccgtaaac caccgcgccc agcatcacgt cttcgggcag gattttggct
     5461 tcggattcca ccatcaacac ggcttttgaa gtaccggcga ccaccaagtc caattgcgat
     5521 ttagccaatt cggctttagt cgggttcaaa acgtacacgc cgtttacata acccacgcgt
     5581 gccgcaccaa tcggaccggc aaacggtacg ccgctcaaca ccagcgcggc agatgcgccc
     5641 aacattgcag gaatgtcgga atcgatttcc ggatcgacag acaccaccat cgctacgatt
     5701 tggatgtcgt ggtagaaacc ttcagggaat aaaggacgaa tcggacggtc gatcagacgg
     5761 ctggtcagga tttctttttc gctttgtttg ccttcgcgtt tgaagaagcc gccggggatt
     5821 ttgcctgcgg cgtaagtacg ctcgaggtaa tcgacggtca gggggaagaa gtcttgacct
     5881 tctttcactt ctttattggt ggtaacggca accaaaacga cggtgtcgcc catagatact
     5941 ttaacggcag cggcggcttg gcgggcgatt tcgccggttt ccaaagtaac ggtttgatta
     6001 ccgtattgga aggtcttaac gtgtttattg aacattattg ttcctttcaa aataccgcac
     6061 tgctaaaaca ctaataatgc acactaaaat ccgaatgtgc atagttaggg tttcagaccg
     6121 tgcggcaggt tataaacaag ctttcagacg gcactccgga cgctgaaaac agcccccgat
     6181 tataaaggca accatcctta aaatccagta ttgatacaat aaaaaggccg tctgaaccat
     6241 atttctttca gacggccttc aaacttaaaa atcaatcctg agtcagcaaa gtcaatgctt
     6301 cctgatattt ttcgactgtt ttttgaatca cattggcagg cactttaggc gcaggggctt
     6361 ttttgttcca accgctttgt tccagccagt cgcggacaaa ttgtttgtca aaagacggcg
     6421 gatttgtgcc gactttatat tggtcggcag gccagaagcg gctcgaatcg ggggtcaata
     6481 cctcatccat cagcgtcagc gtaccgtttg tatccaaacc gaattcaaat ttggtatcgc
     6541 aaatgatgat gccgcgcgat ttggcatatt ccgccgcttc tgtataaagc tgaatcgctt
     6601 tggcgcgcac ttccgccgcc aattcttttc cgataatgcg ttcgcattct tcaaagctga
     6661 tgttttcatc gtgatcgccg actgcggctt tggttgaggg cgtaaaaatc acttcaggca
     6721 gttgttgcgc ttcctgcata ccttcaggca gttgaatacc gcaaaccgag ccggtttttt
     6781 gataatcttt ccaaccgctg cctgccagat aaccacgcac aatcgcctct actttcaccg
     6841 gagtgagctt tttagccacg acggcgcgtt tctctaaagc tttggcttcg ttttcaggca
     6901 aaacatcgta aaccgtttga ccggtaaagt ggttgggcat aatatgcacc agttttttaa
     6961 accaaaaatt ggaaatctgc gtcagaatct cccctttgct cggaatcggg tcgtccaaaa
     7021 tcacatcgaa tgcagacagg cggtcggaag cgaccatcag catacgttta tcgtcgattt
     7081 catataaatc gcgcactttt ccaaaataga tctttaccaa accaatctca ctcatttcgc
     7141 cccccctgaa aatatcttga aaataccgac ccgacacccg acaggtttga atcacaaacc
     7201 aatattctag ccgaagtcgg cgcaaaacaa tacccatggc acaaaaaacc aacccgtcaa
     7261 ccgtcggcaa agttttggca ctataatacc gacagcaagt cctacaatac acttttacca
     7321 aaggaaatac ctcatgagaa tcctattgac aggctcgaaa agccaactgg cacgctgcct
     7381 gcgcgaccgt cttccggaag actgggaaac cattgcgacg gattccgcat ccctagacat
     7441 taccgatgcc gatgccgtct gcaacatggt caaaagtttc caacccgacg ctattgtcaa
     7501 cacggctgcc tatactgccg tcgacaaagc ggaaggcaat gcggcagcgg catttgccgt
     7561 caatgcttcc gccgtttaca accttgcctt ggcagcacat cgcgcccatg cccgattcat
     7621 ccacatctca accgactata tctttgacgg taaagggaaa aggccctatc aggaaagcga
     7681 ctttaccaat ccttccaatg tatacggaca atccaaaacc gcaggcgagc tgctcgcact
     7741 gtctgccaat cccgacagcc ttatcctgcg gacttcttgg ctgtttagcg aatacgggga
     7801 caactttgtc cgcacgatgc tgaaccttgc gcgggaacgt tccccgctgt ccgccgtcca
     7861 caaccaaatc ggctgcccga cctatgccgg cgatttagct gccgccatca tccgcctttt
     7921 gcagcaaacg tcgcccgtgc gcggcattta ccactacgcc ggcggcaaat ccgtatcctg
     7981 gtacgaattt gcccaacata ttttccaagc ggcatcgcaa cagcagacat ccttccccgt
     8041 tcccgaattg actgccgttt cagacaagga atatccgacc gccgccccca ggcccgcata
     8101 cagcattttg gactgccgca aaatcgaaaa cgacttcagc atcaaaccgt cagactggca
     8161 aaaagccctt gcacaggtcg tttccaagct gctctgatgc cgcccgcctc tccgtttccg
     8221 ccgtcaagca ccgccttggc ggttttc
//
LOCUS       DAWFAD010000085         8238 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWFAD010000085 DAWFAD010000000
VERSION     DAWFAD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.085"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(107..1030)
                     /gene="era"
                     /locus_tag="WGO49_001386"
     CDS             complement(107..1030)
                     /gene="era"
                     /locus_tag="WGO49_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ0306490.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELLKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
     gene            complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WGO49_001387"
     CDS             complement(1077..1796)
                     /gene="rnc"
                     /locus_tag="WGO49_001387"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ0306491.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRRADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            1963..2286
                     /locus_tag="WGO49_001388"
     CDS             1963..2286
                     /locus_tag="WGO49_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ0306492.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNRPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADIAKTNPETAVLLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            2333..2809
                     /locus_tag="WGO49_001389"
     CDS             2333..2809
                     /locus_tag="WGO49_001389"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ0306493.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNLASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            2887..3312
                     /gene="nusB"
                     /locus_tag="WGO49_001390"
     CDS             2887..3312
                     /gene="nusB"
                     /locus_tag="WGO49_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ0306494.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(3680..4714)
                     /gene="pyrC"
                     /locus_tag="WGO49_001391"
     CDS             complement(3680..4714)
                     /gene="pyrC"
                     /locus_tag="WGO49_001391"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ0306495.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAVLPEGSAFEPLMTLYLTDQATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQDILFLVHGEVTDPEIDIFDREAAFIERVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAASVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPVSVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(4774..5379)
                     /locus_tag="WGO49_001392"
     CDS             complement(4774..5379)
                     /locus_tag="WGO49_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306496.1"
                     /translation="MPSEMRMSRLMLLLSAFVLAACNAQNRQSGVRHQDFARVAVDLF
                     RSACVLTQGSFESVSKFAAVGHFVPVGKEDLSHLPPTVAEPDVQALWTLERDGGIYYL
                     SLTRDSCSVKAERADSAALLEHFDGLVRQPPKNANIELRAEQSVQASFEIRQVSYAWR
                     EAGSSEETVLTAQTADSPDLPVQAVLNLTHRSHNGKPLILP"
     gene            complement(5380..5604)
                     /locus_tag="WGO49_001393"
     CDS             complement(5380..5604)
                     /locus_tag="WGO49_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ0306497.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVKHK"
     gene            5713..6324
                     /locus_tag="WGO49_001394"
     CDS             5713..6324
                     /locus_tag="WGO49_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HEZ0306498.1"
                     /translation="MRRTALLLLALTAGTSLAAGFNQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGDVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            complement(6384..7256)
                     /locus_tag="WGO49_001395"
     CDS             complement(6384..7256)
                     /locus_tag="WGO49_001395"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ0306499.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPTDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRREMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(7293..8078)
                     /locus_tag="WGO49_001396"
     CDS             complement(7293..8078)
                     /locus_tag="WGO49_001396"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ0306500.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAENA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
BASE COUNT         1988 a         2499 c         1959 g         1792 t
ORIGIN      
        1 tgcacagata atatcaaccc gtttttaaca aagatattcc cgattgaaac aaaatgccgt
       61 ctgaaacctg ttcggacggc atttgcgtaa agttcagcaa gaaaaactac aaacccagct
      121 cgcgcaggaa gcggatgtcg tccgcccaac cggatttgac tttgacccag accttcaaaa
      181 atactttggt atcaaacagt ttttccatat ccaaccgcgc ttcggtggaa attttcttca
      241 aacgctcccc gcctttgccg attaaaatcg ccttttggct ttccttatcg accaaaacgg
      301 cgatatagat gcggttcaaa ccgtcttcct cttcaaactg ctccacttcg acgttcatcg
      361 cataaggcaa ttcctcgccc aaatagcgga acaatttttc acgcacgatt tccatcgcta
      421 aaaaacgcgc cgatttgtcc gtaaccatat cttcgggata catcggcacg ctttcgggca
      481 gatacggctt gagcagctcc aacaggttgg caatccgcaa tccgtgtttc gcactgaccg
      541 cctccgccgc cgcaaattca aattcggcgc gcacttgggc aacaaacgcc tccagcgcgt
      601 aacggtcttt cgccttgtcc ttgtcgattt tgttgaccac taaaatgacc ggcgtgtgct
      661 tgggcagttg tttcaacacg acacggtcgg catcggtaaa gcgcatcgct tccacgacga
      721 aaaccaccac atccacgccg ccaagcgcct cggtaacgtt ttgattcagc ctgtcgttga
      781 gcgcgttgcg gtggtcggtt tgaaagccgg gcgtatcgac aaacacgaac tgcgcggtat
      841 cgtcggtata aatccccgtt acgcggttgc gcgtcgtctg cgcctttttg ctggtgatgc
      901 tgattttctg accgatgaga tggttcatca gcgttgattt gcccacgttc ggacgaccga
      961 caatcgccac gaagccgcaa cggtatccgc cggcggcgcg ttcccctgca aggaaggttt
     1021 caatatccat attcgctttc atatccacat ttcagacggc atattcacgc gccgcctcat
     1081 ttctttttct tcttcagcgg cagcttctct tccagccatt tcaaagcctc tttcgccgcc
     1141 tcctgttccg ccgccttgcg gctcgtccct ttggcacggc acacgaaacc cagttcgccc
     1201 aaatcgcagg aaaccacaaa catactgtcg ttggcatggc cgatttgctc ttcgatacgg
     1261 tatttcggca aagcaaagcg gcgtgcctgc aacgcctcct gcaaagcagt tttgccgtct
     1321 tttgcctgat tttgaaaatc ggcgcgccgg acgcgatcgg caaacaaatg gcgcaccacc
     1381 ttttccgccg tgttgaaatc ggcatcgaag ctgacggcag caaacatcgc ctccatcgcg
     1441 tctgccagta tcgaaggccg tctgaagccg ccgctcttca actcgcccgc ccccaagtac
     1501 aggccgtcgc cgacattcat ttccgccgcc atttccgcca gcacgccctc attgaccaga
     1561 cttgcccgca accgcgacaa ctcgccctcg gtcaacttcg gaaacgcgtc aaacagcatc
     1621 cgcgccaccg tataattcaa aatcgaatca ccgacaaact cgaaccgctc gttgtgcttc
     1681 gcatgatggc tcctgtgcgt caaagcctgc cgcaaaagcg aaatatcgcg gaacgcgtag
     1741 cccagtttct tctgtatcgc cgcgtgtgcc tgctgtttca aaacatcgtc tttcattacc
     1801 gtcttccgca aaaaacagcc cgaatgcgtt tccatactcc aaaaatacag aaacacctta
     1861 aggctgcaaa cggcgcacat tgtaccacca aaccgccgcc gtctgccgac aacgcacgaa
     1921 tcgtggtaaa atccgtaagt tttcccccaa cgtattcccg taatgaacgc atttgcacaa
     1981 gcactttcct cggcactcga ccgctgtatc gccaccaaca ccgtcgccaa acaaaaccgg
     2041 cccgtcggct tcctctaccg cgaagccccc gtttttgaaa acgacagcgg ctggcgtttc
     2101 ttcagcggcg acgagaccga cgaatacacc gacgatcccg acaacttcag catcgtcagc
     2161 ctcgccgaca tcgccaaaac caaccctgaa accgccgtac tgctttccca acctgaaggc
     2221 agcgcgtggg aattggcaga agacggcaca ttccaaaccg tcgccgactg gcaaccgcaa
     2281 gactgaaacc ttcagacggc attccgaaac cccgaatacc aaaggacacc caatgaacac
     2341 catcgccccc aaccttgacg gcaaacacct ccgcatcggc atcgtacagg cacgtttcac
     2401 caacgaaatc ggcagcgaaa tgctcaaagt ctgctgccgc accctccaag aattgggcgt
     2461 ggcagacgaa aacattaccg tcgccaccgt acccggcgcg cttgaaatcc ccatcgcgct
     2521 gatgaacctt gcctcttctg aaaaatttga cgcactgatt gccatcggcg tcgttatccg
     2581 tggcgaaacc taccatttcg agctggtttc caacgaatcc ggagcagggg tcagccgcgt
     2641 cgcactcgac tacaacatcc cgattgccaa tgccgtcctg accaccgaaa acgacgcaca
     2701 ggcaatcgaa cggattgaag aaaaagcctc ggatgccgcc aaagtcgccg tcgaatgcgc
     2761 caacctcgtc aacctgctgc tcgaagaaca gtttgaagac gaagaataac aaacagggcg
     2821 tacaccaata cgccctttcc gtttcagacg gcataccgtg ccgcccaacc gtttcaagga
     2881 aaaatcatga aaacagcccg ccgccgttcc cgcgagcttg ccgtacaagc cgtttaccaa
     2941 tcccttatca accgcaccgc cgcgcccgaa attgctaaaa acatccgcga aatgcccgac
     3001 tttgccaaag cggacgaaga attgttcaac aaacttttct tcggtacgca aaccaacgca
     3061 gcggactaca tccaaaaaat ccgccccctg ctcgaccgcg acgaaaaaga cctcaatccc
     3121 atcgaacgcg ccgtcctgct gaccgcctgc cacgagctgt ccgccatgcc cgaaacgccc
     3181 taccccgtca ttatcaacga agccatcgaa gttaccaaaa ccttcggcgg cacggacggg
     3241 cacaaattcg tcaacggcat cctcgacaaa ctcgccgccc aaatccgccc cgacgagccc
     3301 aaacgccgtt gatgcatcac aaatttaaag aaagttctat gtccttattt ctgctttctt
     3361 tgtttcgtca ttcccacgca tgtgggaatt tggttttttg aattttgggt atttcaaata
     3421 aattctgaaa cttcaatctc gtcattcccg cgcaggcggg aatccggttt tttgagttgc
     3481 tgccgttttt gataaactct agtcgcatcg aatttccaga ttcctgcctt cgtgggaatg
     3541 gcgaaatttg aaaattgtgg gaatttatcg gaaataaccg aaacgtaaca aactcaaacg
     3601 ccgttgacgt atcaatcagt cagcaacaca aatgccgtct gaaagccctc ccttcagacg
     3661 gcattttgtt tccagcccat caatactgca ccgtccagcc gatttcgccg cccgcgcgca
     3721 tcgggacaag ttcgccgtcg ccgtagggaa cacttacggg gacggtttgg ctttgtttga
     3781 cgagggtgat cgtgccggcg ttttccggaa tgccgtaaaa ccttgcgcca tttttcgagg
     3841 caaaggcttc gagtttgtcc aacgcgcctg ctttttcaaa cacttcggcg taaagctcga
     3901 cggcggtcat cgcgctgaac ataccggcgc agccgcaggc gttttctttg gcggacttgg
     3961 cgtgcggcgc ggagtcggtg ccgaggaaga atttgtgcgc cttctcgccg gtaacggcgg
     4021 caaccaatgc ctgacggtgg gtttcgcgct tgaggacggg caggcagaaa tgatgggggc
     4081 gcacgccgcc gaccaagagg tcgttgcggt tgagcaggag gtgttgcggg gtcacagagg
     4141 cggcaacgtt gtcgcctgct tccaaaacca ggcgggcggc ttcggcggtg gtaatgtgtt
     4201 caaacacgac tttaagattc ggcacttgcg ccaaaacggg tttcatcacg cgctcgataa
     4261 aggcggcttc gcggtcgaaa atgtcgattt cggggtcggt tacttcgccg tgaacgagga
     4321 acaggatgtc ctgttttgcc atttcttcca acacggggat gagcttgaat aggtcggtta
     4381 cgccggaatc ggaattggtc gtcgcgccgg cggggtagag tttgaaggcg acgatgccgg
     4441 cggctttggc ttcgcgcacc agctcgggcg tggcttggtc ggtcaggtaa agcgtcatca
     4501 gcggctcaaa cgcgctgcct tcgggcaaca ccgccataat gcgcgctttg taggcaagcg
     4561 cgtcggctac actgacgacg ggcggtttga ggttgggcat aatgacggcg cgccccattt
     4621 ggcgggcggt ataaggggca acggctttga gcgcgtcgcc gtcgcgcagg tgcaggtgca
     4681 tatcgtcggg gcggataatg gtcagggttt gcatggtttt cctttgtttt atttgaatgt
     4741 tcagacggca tgccgacttt atgccgtctg aagttagggg agaattaggg gtttcccgtt
     4801 gtgggaacgg tgcgtcaggt tcaaaactgc ctgtacgggt aaatccgggg aatcggcggt
     4861 ttgtgcggtc aatacggttt cttcggagct gcctgcttcc cgccaggcat agctgacttg
     4921 gcggatttca aatgatgcct gcaccgactg ttccgccctc aattctatat tggcattttt
     4981 cggcggctgt cggacaagtc cgtcaaaatg ttccaacagc gcggcactgt ctgcccgctc
     5041 ggctttcaca ctgcaactgt cgcgcgtcag gctcaaataa taaatgccgc catcgcgttc
     5101 aagcgtccac aacgcctgta catcgggttc tgcaacagtg ggcggcagat gggacaggtc
     5161 ttcctttccg acagggacaa aatgtccgac agcggcaaac ttggaaaccg attcaaaact
     5221 gccttgagtc aggacgcagg cactccgaaa caaatcgacg gctacacgcg cgaaatcctg
     5281 atgccgtaca ccggattgcc ggttttgggc attacacgca gcaagcacaa atgccgacag
     5341 caataacatc aatcgggaca tccgcatttc agacggcatt tatttgtgct tgacgaccag
     5401 cgtgaacacg ccgtcctgtt cggaagcatc caacagcaca tgaccggttt ggcggcaaaa
     5461 agcctcaaaa tcccccggtg cgccgccgtc ggttgccaga acggtcaaca cgtcgccctg
     5521 ctgcatttgc gccaaagcct ttttcgcccg caaaatcggc aggggacatt tcaatccggt
     5581 tacgtctaaa gtttcgctgt tcatcgtttg taccgtttaa aaaatgggaa tcggggggag
     5641 attatacccc agttgcttgc agcccccttt gccaacccat aaaataacgc cgttttcaat
     5701 ttggagaaac ttatgcgccg caccgccctc ctgctgctcg cactcaccgc cggcacgtcc
     5761 cttgctgccg gcttcaacca aaaagacacg ccgatcaaca cccgctaccg cgaaacgccc
     5821 gaagaagccg ccgcccgcga atttaaagaa cataccgcca aactgccgcc gctgcccgat
     5881 acgcactcgg acggctggtt cgacatttat gtggatgaaa attacggcaa acagccgaaa
     5941 atcctgctcg acagcctgca aattatgccc gcgcccgacg gcagcatccg ctacattctc
     6001 aatatccgct ccgacaaagg ctacgacaac ctgactgccg aaggcatatt ctgcgcccgc
     6061 ttctccatca ggttcggcaa cggcaaacct tcctcctata aagtgttcgg ctacggcgac
     6121 acggtcaacc gccgctggat acagccccga aacgccgaat ggaaaccgat aggcggtaca
     6181 ttaagccgca acgacgcact gcgcaccgtc ctgtatcagg cattctgcga gggcgatgta
     6241 cccgccgaca cgaaaggttt ggtacaacgc ctgaaagaac gtgccggacg ctatgccccg
     6301 tcgataaagc cgcacaacaa ataaccagac acaaaaatac cgtctgaaac atcggtttca
     6361 gacggtattc gattcatcag ccatcaagcg gcggaaactt tgtcctgacg gcacaacagc
     6421 gtaatcaaat cactgatgcg ccgcttcatc tcacgccggt caacaatctg gtcgattgcg
     6481 cctttctcca gtaaaaactc agcacgctgg aagccttccg gcaaggtttc ccgtacagtc
     6541 tgttcaataa cgcgcggtcc cgcaaaaccg attagggcat tgggttcagc caatacgaca
     6601 tcacccaaaa atgcgaagct ggcggatacg ccgcccatag tcggatcggt caacaccgat
     6661 ataaacggca ggcgtttttc cgtcagcaaa tgcagcgcgg cactggtttt cgtcatctgc
     6721 atcaatgagt tcacaccctc ctgcatacgc gcaccgccgg aagccgccac acagacgaac
     6781 ggacaattgt cggcaaccgc ccggcggacg ccctgtacga atcgttcgcc cacaaccgaa
     6841 cccatcgaac cgccgataaa gcggaactca aacgcagcaa ccacaacggg cagaccgttc
     6901 atcttgcctt tcattaccac caaagcatca tcttccccgg tcagcttgcg tgccgcactc
     6961 aaacgatccg gatatttttt gccgtcttta aacttcaaag gatctgtcgg tttgacattt
     7021 ccggcaacct cctctctgcc atcctcatcc aaaagcagat tcaggcgttg tcgtgccgac
     7081 aacggattgt ggtggttgca tttcggacaa acctgattgt tctgctgcaa ctcggtcgaa
     7141 taaacggttg ccgaacaaga cggacatttg tgccacaaac cctcgggaac attggaagaa
     7201 ccgtctttgc tgcggttttt gattttgggt ggcaggattt tatctaacca gctcatggac
     7261 aactcctaaa atatttagaa tgcgtgacag aatcagcgca cggcatcctt caactctttg
     7321 accaaagcac cgacggccgc agcctcgttg cccgcattgt tttcaatctc tttcacaatc
     7381 cggctgccga caatgaccgc gtcggcaacc cggccgattt tgcgcgcgtt ttccgcattg
     7441 ctgatgccga aaccgacacc gatgggaata tcgatatact gatgcaaata ctctatttta
     7501 cgcgaaacct catccgtatc caaacttgcc gcgcccgtta cgcccttgag cgaaacataa
     7561 tagacaaatc cgcctgccag ctcggcaatg gttttaatac ggtcttccgt cgtcgtcggc
     7621 gcaatcagga aaatacagtc gaccccgtta tccttcagct cgcgatagag cggatcgatg
     7681 gtttcgacag gggaatccac cgtcaacacg ccgtccacac ccgccattgc ggcttcctga
     7741 gcaaactccc gataacccat cttatgtaca gggttcaaat atcccatcaa aacaaccggc
     7801 gtttgcgtgt cggtttcacg gaattttctg acgacatcca agacatcgcg cagcgaaatc
     7861 ccgtttgcca acgcccgctc cgccgcacgc tgaataaccg gtccatccgc catcggatcg
     7921 gaaaacggca cgcccaactc caaaatatcc gcaccgtttg caaccatgcc gtgcatcaat
     7981 gccaaagttg tccgaatatc ggggtcgccc accgtaatat agggaatcaa tgcctttccg
     8041 ccatccaaag cggcaaaagc ctgccggatt ctgctcattt gtctcttcca tcctatcggc
     8101 aaatatgcca taaaacacaa tcagtaaatt tttcccagta taacatttgc ggtacggata
     8161 ggcgaatacg gcaaacagaa accacgcaaa tcagagccgc cgtttgaacg aggcggaaat
     8221 aaacggcaag catctcgg
//
LOCUS       DAWFAD010000086         8222 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWFAD010000086 DAWFAD010000000
VERSION     DAWFAD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.086"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(63..623)
                     /locus_tag="WGO49_001397"
     CDS             complement(63..623)
                     /locus_tag="WGO49_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ0306501.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
     gene            complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WGO49_001398"
     CDS             complement(1040..2080)
                     /gene="mutY"
                     /locus_tag="WGO49_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ0306502.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            2243..3284
                     /locus_tag="WGO49_001399"
                     /pseudo
     CDS             2243..3284
                     /locus_tag="WGO49_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            complement(3542..4180)
                     /locus_tag="WGO49_001400"
     CDS             complement(3542..4180)
                     /locus_tag="WGO49_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ0306503.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAIDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEVQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            complement(4361..6196)
                     /gene="edd"
                     /locus_tag="WGO49_001401"
     CDS             complement(4361..6196)
                     /gene="edd"
                     /locus_tag="WGO49_001401"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ0306504.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYASMPKSIKIEMLQDTVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLDAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKAGNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLSAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVSGELNVLIDEAE
                     WNAREVEHIDLGANQQGCGRELFAGFRSLTGGAETGAMSFGGEFA"
     gene            6712..8157
                     /gene="zwf"
                     /locus_tag="WGO49_001402"
     CDS             6712..8157
                     /gene="zwf"
                     /locus_tag="WGO49_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ0306505.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAARGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALESRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
BASE COUNT         1923 a         1951 c         2270 g         2078 t
ORIGIN      
        1 ctaaatatct agcaaaaaag tgcggtcaaa attcaccgca cttttcgttt cgaacaatcg
       61 aattatttaa tcacgccaca tgccatacgt gggccgccac cgccaagtgg agctggatga
      121 tcggagtgat tatcaccacc cgtgtggatc ataatagagt gaccgcgaac atcatctaaa
      181 tgtttaagac gtggtgctaa aacaggattt gttgctgtgc catcatgcaa tacagttaat
      241 gcaggtaaat cacctaagtg tgcatcatct tgccatgggt aaccatgttg ttttgcacct
      301 ttaggatccc agtgaccgcc tgcgcctaaa ccagctgtca atttaccttc tttttctttt
      361 ggctcacagc ttgggttttc atggatgtgg aaaccatgta agccttcgct taatccttgt
      421 aaatcagggg taaacacaag accatagtta gattcagtaa tagtcactgt acccacatct
      481 ttgttaccgt ttactggatc aagttgttgc actttcactt caatagaagc acctttaggt
      541 atcgtattat gctcgtgtgc ttgcgcaaca ccaactgaac atactgcact aaccgctagt
      601 gctaataagg ttttcatatt cattgcaatc tcctttattg cagtaaaaac tcgcgactat
      661 tttagtcaat tttgttttaa aagccgataa atttttacga aaattttaca ttaattgtta
      721 tttgcagaaa attagaaccc gttatcacgt cttggataaa tgccaaaacc attgtgattc
      781 agagaattaa aaagaaaaat ttcggaatcc tcatcctttt ctgaaacctg attgcggtat
      841 ccatggcttt gtctttgtca tttaagtttg tgttcatgtt tttgaccttc tttgcttttt
      901 aaatcctttt taaattgccg acttcttccg ttccaaatgt cccatttgaa cattatcccg
      961 ccgcctaaaa tacgacccaa attcatcggt tgttcttgct gcgatacctg caaacaacag
     1021 ttcaatgggt ttattttgtt tattctaacc tattaccgtt taaataaatt tccaaaggct
     1081 tgggcaggcc gtaatctttc aaatgcgtcg gctttatcca aatgccgtct gaagggcttt
     1141 cagacggcat ttgtccttca aacggcgtaa tcatcagcaa ccggtgcgtc agccggtggg
     1201 tcagggcggt ttgttcgtcc atatctgcca tggtcaggga gaatttggcg gcaaagtcgg
     1261 aaagcccgtt caaactttca aaacacggca cgcaatacag cccgccccaa atgcctttgg
     1321 cggggcgttt ttccagcaaa atcgcgccgt tccggttgcg gataatcagc cagtaaagcg
     1381 gcagggtcgg tacttcggcg gcggtttttt tgcgcggcag ctcggcggtg cggttttgct
     1441 tttttgcttc gcagatgtcc gccatcgggc attggtggca caagggtttc gtccgtttgc
     1501 acacggttgc gcccaaatcc atcaaacctt gtgtataggc aggcatatcg gcgttttcag
     1561 acggcagcag gctttcggca agtgtccaga gcgagttttc aaattttttg tcctgcggat
     1621 tgccgtcgcg ggcgaacacg cggcagagta cgcgtttgac gttgccgtcc aaaatggttt
     1681 cgcggcggtt gaaggagaag gcgcaaatgg cggcggcggt gcttctgcct acgccgcaga
     1741 gcgtttccaa gtctttgcgt tccgatggaa acgtgccgcc gaattgcctg acgacttgtt
     1801 gcgcggcttt gtgcaggttg cgcgcgcggc tgtaatagcc caagcccgcc cacaacgaca
     1861 acacttcgtc ttgcggcgcg gcggcaagcg tctgaacggt cgggaatttt tctaagaaac
     1921 gcggatagta gtccaacacg gtggcgacct gcgtttgctg gagcatgatt tcggaaagcc
     1981 agacgcaata agggtttttg acctgccaag ggaggtggtg gcgaccgtgt tgtttttgcc
     2041 agcggatgag ccgttcggag aaggggatgg gtgtgttcat taatatcaat cggtggtttt
     2101 atttatattt aaaacagtat gttattgtat aacattgtga aaataattct tattgactta
     2161 ttttttgtag gggtatataa ctcatataaa gaaactttat tgcggtattg aaattattta
     2221 tcaacaagca aggagtatca gcatgaaagc aatggtttat cacggcgcaa acgacatccg
     2281 ttttgaagaa aaaccccgtc cgcagattat cgatccgacc gatgcggtgg tgaaaatcgt
     2341 caaaaccacg atttgcggta ccgatttggg tatttggaaa ggcaaaaaaa accgaagtcg
     2401 ccgacggtcg tattctcggt catgaaggca tcggtattgt agaagaagtc ggcgaggctg
     2461 taaaaaacat caaagtcggc gataaagtca ttatttcatg cgtcagcaaa tgctgtactt
     2521 gcgacaactg caaaatccaa ctttattcgc attgctgcaa cggcggctgg attttgggct
     2581 acatgattga cggcacgcag gcagaatacg tccgcacgcc ttatgccgac aacagcctcg
     2641 tgccgctgcc cgacaacgtc aacgaagaag tcgccctgtt gttgagcgac gctttgccga
     2701 ccgcacacga aatcggcgtg caatacggcg atgtcaaacc cggcgacacc gtattcatcg
     2761 caggtgccgc tcctgtcggt atgtccgcct tgttgaccgc ccaactgtat agccccgccg
     2821 ccattatcgt gtgcgatatg gacgaaaacc gtttgaaact ggcgaaagag ttgggcgcga
     2881 cccataccat cagccccgcc tcaggcgatg tctccaaaca agtttttgcc attgtcggcg
     2941 aagacggcgt agattgtgcc atcgaagccg tcggtattcc tgcgacttgg aatatgtgcc
     3001 aagacatcgt gaaaccgggc ggtcatatcg ccgttgtcgg cgtacacggc caatccgttg
     3061 atttcaaact ggaaaaactc tggattaaaa acctcgccat cactaccggt ttggtcaatg
     3121 ccaatactac cgaaatgctg atgaaggcaa tttccagcag ctcagtcgat tacaccaaaa
     3181 tgatgaccca ccatttcaaa ttcagcgaat tggaaaaagc ctacgacgtg ttcaaacacg
     3241 ccgccgaaaa ccaagcgatg aaagtggttt tggaagcgga ttaatcggcc ttgaatcagt
     3301 tgttttttta agcggatgcc gtctgaatgc gtttcagacg gcattttttc agccgcgtag
     3361 tatgggctgt ccggctgctg tcgaatgaac tgaacgttat ttttattatg cggaaggcgg
     3421 atgggcattt taggggcggt atgtctgatc agtgctgccg tgtatgccga ctgtgccctc
     3481 aatcggcaaa atgccgtctg aaacgggaaa ggtttttcag acggcatttt tacgatgcga
     3541 atcaggcttt gggtttcaac gccgccgctt ctttggcgag gcgggtgatg gtgtcccagt
     3601 ctttgttttt cacggcttct ttcggtgtca gccaagagcc gccgacgcac aggacgttgg
     3661 gcagtgccaa gtactcgggc gcggtggcga ggctgatgcc gcctgtcggg cagaagcgaa
     3721 catcggcgta aggaccgtac agggctttga gcatggcttt gccgccaacg acttcggcag
     3781 ggaagagttt gagggtgtcg atgccgtgtt ctaaagccag ttggacttcg cccggggtgg
     3841 caacaccggg aatcaggggg atgccgctgt tgtggccggc tttggcgagg gattcgtgca
     3901 gccccgggct gatggcgaaa accgcgcctg cgtcttcgac ggctttgagc tgttcgggat
     3961 tggttaccgt acccgcgccg acgatggcgt tgggcacttc tttggcaatc aggcggatgg
     4021 cgtcgaggcc gacaggggtg cgcagggtga tttcgagggt agggatgccg ccttcgacaa
     4081 gggcgtggga caaatcgatg gcggtgctta agtcgtcaat cgccattacc ggcacaactg
     4141 cgccggcggt caaaatttcg cggggggtca gtttggacat ttcggttctc cgggtggaat
     4201 ggggtatttt attaagatgg gacaggttgt aggtcggatt caagaaaccg acatcttgga
     4261 cggctgtgtc ggattcgaga atccgaccta cgtctatctt aaaacgcttg aataattttt
     4321 cagacggcct tcggtctgaa aaggccgtct gaaacgcaca tcaggcaaat tcgccgccga
     4381 agctcatcgc gccggtttcc gcgccgccgg tcaaactgcg gaagccggcg aagagttcgc
     4441 gaccgcagcc ttgttggttc gcgcccaagt cgatgtgttc gacttcgcgg gcgttccatt
     4501 cggcttcgtc aatcaggacg ttgagttcgc cgctaacgga gtcgaagcgg atcaggtcgc
     4561 cggtacggat tttggcgatg ttgccgccca tcagggcttc gggtgtcatg tggatggagg
     4621 ctggaacttt gccggacgcg ccggacatac gaccgtcggt cagcagtgcc actttgaagc
     4681 cgcggtcttg caggatgccc aaaggcgggg tcagtttgtg caattcgggc ataccgttgg
     4741 cacgcgggcc ttggtagcgg acgacgcaga cgaaatcgcg ttccaactcg ccgcgttcaa
     4801 atgcggacaa tacttcgcgt tggtcgttga acacgatggc aggtgcttcg atgatgcggc
     4861 agccttcgcg cacggcggac actttaatca cgccgcgacc gatgttgcct ttcatcaggc
     4921 gcagaccgcc gtcgggggag aacgggttgc cggctttgcg caggatgtcg tcgttgccgc
     4981 tggtttcggg ggcttcgcgc cattcgagtt tgccgtcgat aaggaaaggc tctttggtgt
     5041 agtggcgcat accgtgtccg acaacggtat cgacatcgtc gtgcaacagg cctgcgtcca
     5101 gcaattcgcg gataacgaaa ggtagaccgc cggcagcggt aaagtggttt acgtcggctt
     5161 tgccgttggg gtacacacgg atcagcaatg ggatgatgga agaaatttcg tcaaagtcgt
     5221 cccagttcaa aatcacgccg gcggcgcgtg ccatggcaac gaggtgcatg gtgtggttgg
     5281 ttgagccgcc ggttgccatc aggccgatta gggcgttaat gaaggatttc tcggtcaaca
     5341 tttcgcccaa tggtttaatc gtgccgtttt tgatgccgcg tgcgaggtgt ccggcggcat
     5401 agcgggtcag ggcttcacgc aggtcggtgt aagggtggac gaaggcggcg gcaggcaggt
     5461 gcacgcccat catttccatc atcatttggt tggagtttgc cgtgccgtag aaagtgcagg
     5521 tgcccgggct gtggtaagaa cccatttcgc ttttcaaaag ttcgttgcgt ccgaccttgc
     5581 cttcggcgaa aagctggcgg gtgcgggctt tttctttgtt gccgataccg ctggacatcg
     5641 ggcctgcggg gacgaagata cccggaatat ggccgaaaga caacgcaccg atcatcaaac
     5701 cgggaacgat tttgtcgcac acgcccataa acaggctgcc gtcaaacatt tgatgagaca
     5761 gaccgacagc agtactcatg gcaattacgt cgcgggagaa cagcgacaat tccatgccgg
     5821 catagccttg ggtaataccg tcacacatgg cgggcgtacc gccggcaact tgcgcggttg
     5881 cgccgttttt ctgcgcttcg tctttgattt ggtcagggaa gtctttaaac ggctggtgtg
     5941 cggaaaccat gtcgttgtag gcggtgatga tgcctaagtt gggaacggtg tcctgaagca
     6001 tttcgatttt gatgcttttg ggcatggagg cgtagccgtg cgccaaattg ctgcagccga
     6061 gttggttgcg ctctaagcgt cccatctgtt tggcactacg gattttcgcc agatattttt
     6121 cacgcgtcgg acggctgcgt tcaataatgc gcccggtgat ttcggcgagt ttggagtgga
     6181 taggagtagt gttcacggtc ggtctcctgt cggaacgggg ggtattgggg atttgtttgt
     6241 gcgtttctga tttttgtatc gggtattcag acggtgagta tagtggatta acaaaaacca
     6301 gtacggcgtt gcctcgcctt gccgtattat ctgtactgtc tgcggcttcg tcgccttgtc
     6361 ctgatttttg ttaatccact atattttaaa aggtataccg tctgaaagtg ggaagcgggt
     6421 gttattatag tacaaaattt gtaattttgt tacaaaaaaa tcagacatcg aaaagaaatt
     6481 tgatttaaat cgcagaatct tccgattgtt gtaacggaat aggggaaacc tgcccgattt
     6541 ttggactagg tcaaataaat tgtaaaaatg ggaatagaca aaaagtgtag cggtgtgtag
     6601 tattactact cattatgccg cctgcctttg tttccgatat tgcgatgcag cgtgatgggg
     6661 gaaacaggtt tcagacggca tcgaaaaatt ctttcctgac gagataatac gatgagtaca
     6721 caaacaaatt ttgatttggt gttgttcggt gcgaccggcg atttggcgat gcgcaagctg
     6781 ttgccctgtc tgtaccaggc gcatgttgcc ggtctgcttc atcccgaagg gcgtatttta
     6841 ggggtcagcc gcagcgagtt ggataccgaa ggttttctgg cgaaggtgga aaccagttcc
     6901 aaaatccatg tgaaagaaaa tttttccgat gaggcttggg catcgtttgt cgaacgtctc
     6961 gcatatctca aagtcgatgt tacgcagccg gacgattttg ccgctttggg cgatttggtc
     7021 aaagcgcgca aagaaaccga caatgtcgtc atctatcttt ccaccgcgcc gaaattcttc
     7081 gcgcaaacat gtgaaaacct tgccaaaatc ggtttgaacg ccgacaacgt gcgtgtggtt
     7141 ttggaaaaac cgttgggtac ggatttggct tcttcccagc aaatcaatac cgatgttgcg
     7201 cgttatttca aagaagggca aatttaccgc atcgaccact accttggcaa agaaagcctg
     7261 caaaacctgc ttgcgctgcg ttttgccaat gtaatgttcg agccgctgtg gaacaataaa
     7321 tacatcgaaa gcgtgcagtt gaccatcgcc gagcagttgg gcgtggaaga gcgcggcgag
     7381 ttttacgaca ttaccggcgc gttgcgcgat atggtgcaaa accatttgat gcagatgctg
     7441 tgcatgactg cgatggaagc ccccgccagc ttggatgccg acgcggtgcg cgatgaaaaa
     7501 gtcaaagtca tcaagtcatt gaagccgctg accgtcgagt ctgtcaatga aaacgtcgtg
     7561 cgcggacaat ataccgccgc cagaggcatg aacggctatc ttgaagaaat caacgttccg
     7621 caagacagct ttaccgaaac ctacgtcgcc attaaagccg aaatcgaaaa cgaacgctgg
     7681 aagggcgttc ccttctacct gcgtaccggc aaacgcatgg cgggcaaagt ggcggaaatc
     7741 gttttgaact tcaaagattt gaacagccat atttttgaag gcagccgcac cgcgcccaac
     7801 cggctcgtta tcgagttgca accatatgaa tccgtgcgcc tctatacgca gatgaaaacc
     7861 ccgggggcag gaaataaggt cgaaaccgtg ccgctggcaa ccgatttggg caaagcattg
     7921 gaaagccgcc gcgcggaagc ttacgagcgc ctgctgctgg atgtgattaa cggcaaactc
     7981 gctttgttta accgccgcga cgaactcgaa gccgcgtggg aatatgtgat gccgattttg
     8041 gaaaactgga caaataacac cacgccgccg cacggctacg gcgcacactc gtgggggcct
     8101 gaagccgcgc gcgaactatt ggcgcgcgac ggacacaagt ggcacgaaga gcagtaatac
     8161 aataatgcgt tcagacggca tggggtttga aatgccgtct gaacgtaagt aaagcagtat
     8221 ag
//
LOCUS       DAWFAD010000087         8135 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWFAD010000087 DAWFAD010000000
VERSION     DAWFAD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8135
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.087"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(68..292)
                     /locus_tag="WGO49_001403"
     CDS             complement(68..292)
                     /locus_tag="WGO49_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ0306506.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(293..1681)
                     /locus_tag="WGO49_001404"
     CDS             complement(293..1681)
                     /locus_tag="WGO49_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ0306507.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(1767..2588)
                     /gene="prmC"
                     /locus_tag="WGO49_001405"
     CDS             complement(1767..2588)
                     /gene="prmC"
                     /locus_tag="WGO49_001405"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0306508.1"
                     /translation="MTFDEWLGLSKLPKNEARMLLQYASEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYLLGWREFYGRRFAVNPNVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPSLETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(2595..3038)
                     /locus_tag="WGO49_001406"
                     /pseudo
     CDS             complement(2595..3038)
                     /locus_tag="WGO49_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGO49_001407"
     CDS             complement(3103..4587)
                     /gene="tldD"
                     /locus_tag="WGO49_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ0306509.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      4765..4868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4935..6158
                     /gene="cytX"
                     /locus_tag="WGO49_001408"
     CDS             4935..6158
                     /gene="cytX"
                     /locus_tag="WGO49_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ0306510.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRQARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGVSANNISAKLSETPIAVAVAVVGTLLAVLLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            6155..7255
                     /locus_tag="WGO49_001409"
     CDS             6155..7255
                     /locus_tag="WGO49_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0306511.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRGIRCRLNTHTMMQENGSLIVWHGQDKPL
                     SSEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTAWNASAEHGSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVVGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSLARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALVDGKDAPERDEESGLAYIRRQD"
     gene            7276..7893
                     /gene="thiE"
                     /locus_tag="WGO49_001410"
     CDS             7276..7893
                     /gene="thiE"
                     /locus_tag="WGO49_001410"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ0306512.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKTLHGDE
                     LKREIARCVAACQGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAENPEAVVKAFQALWDG"
BASE COUNT         1735 a         2505 c         2205 g         1690 t
ORIGIN      
        1 cttaggtttg catcggcgga atattcaaac acagcctttt ttaaggaaat ccggatacgg
       61 cggcgcatca ataatgcggc ggaatctcgt cgcgcaggga atacggctct tgcgcgtcgg
      121 gattcctgtc ctgcattttt tgatacagca gcctcaactg agcctgctgc aaatccagcg
      181 tctgccgcaa ttccgccacc atcgcgttca ggccggcgat tacgtcctcc tgaagcgcgg
      241 attggatttc cagctctgtg atgcggtgtt ccaactcttg aaccgcgtcc atttacagca
      301 ccatcgcggc aatccagccg gcaatcagca gcgggatatt gtagtggatg aaggtcggga
      361 taacggaatc gcggatgtgg tcgtgttgtc cgtcggcgtt cagccccatc gtcgggccca
      421 acgtggaatc ggacgcaggc gaaccggcat cgcccaacgc ccccgccgtg ccgacgatgg
      481 cgacggtggc aagcggcgaa aaacccaaac cgacacacaa aggcacataa atcgcggcaa
      541 taatcggcaa agtggaaaag gacgaaccga tgcccatcgt taccaaaagc cccaccacca
      601 gcatcgccaa tgccgccata cctttgctgt tgccgaatat cgccatactg ctttccacca
      661 gcggctgaat atgcccggtc gcattcatca cggcggcaaa accctgcgcg gcaatcataa
      721 tgaagccaac catcgccatc atcttgatgc cttcgccgaa tacgtcgttt gccttgtcgc
      781 ggttgatgac gcccaacatc ataaatacgg cgaaaccgag catcgcgccc aacaccagcg
      841 agtcttcata catcaactgg atggcaaagc atacggcaat ggcggcggcg gcggccaggc
      901 tgcggtaggc ggacggctgc ggacggtttg ccgcatcggc gttgcccgcc gtatcggcat
      961 tgttgctttg gtacaggcgc ggtttgcggt aatggacaaa cgccagcagg agtccggcca
     1021 gcattcccaa cgcgggaatc gccattgccg ccattacgtt gatgtttttc acatcaagct
     1081 gcggcgcggc ggaatggatg ttgcccaaca ggatttcgtt caaaaaaatc gcgccgaagc
     1141 cgtaaggcag gaacatatag gtcgtaacca gcccgaaagt aatgacgcac gcaatcaggc
     1201 ggcggtcgat tttcaggcgg ttgaacacca aaagcagagg cggaacaatc atcgggataa
     1261 aggcaatgtg gatggggatg atgttctgac tcatcatgcc catcacaagg atgatggaaa
     1321 gcagcagcca tttgaccgcg ccctcgcccg aacgcacgct gtcgggcata ccgccccggt
     1381 tcagcttgcg gacgaccgcg ccggcaagct gctgcggcag gccggaatgg gtaatcgcca
     1441 ttgcaaacgc gccgagcatc gcataagaaa gcgcaatctt cgcaccgcct tccaaacctt
     1501 tgttgaacac ggggataatc cccgcctggc tgacctgtcc cgccgcatcg gcaatgtttt
     1561 gcagcggcat acccgccacc gcgccgccga caaacgcgcc gaccgtcagg ctcaatacca
     1621 cgtgcacgcg cgacagcgac agcaccagca taacgattac ggcaactacg actgcattca
     1681 ttgtttatcg ctccaaacct ataaatgttt acatatcgaa acacatcata acccaataac
     1741 ggaaaacccg ccaattttgc aaacaattat ttcaaatgct tcatatactt ccccagcgta
     1801 accctgtcca aacccgccaa gtccggcagg gtttccactc ccgaaaaacc attctccgcc
     1861 aacacgccgc gcaccgccgc gccctgatcg aaaccgtgtt ccagcaataa aaaaccgcct
     1921 tccgccaaac ggtcgggcgc gccttgcgcc aaggtgcgga tgcagcttag gccgtctgaa
     1981 aagtcggtca gcgcgatttg cggctcaaac cgcaaatcgc cttgcgacaa atgtttatca
     2041 ccgttttcga tatagggcgg gttggacacg atgatgtccc atttcccttc agacggcata
     2101 tcggtgtcga accacgaacc gtgtgcaaat tcgacccgcg cgcccaaatc cgccgcattt
     2161 ttccgcgccg tttcaaggga gggcgggctg atgtcggatg cgcgcacaaa cgcatcgggg
     2221 cgttcgagcg cgacggttac ggcaaccgcg ccgctgcccg tccccaaatc ccacacgcgc
     2281 ccgttttccg gcaggcgcgc caatacggct tcgaccaaat gttcggtttc ggggcgcgga
     2341 atcagcacat tcgggttgac ggcgaaacgc cgtccgtaaa actcgcgcca gcccaacaga
     2401 taggcaaccg gctcgccgtt cagacggcgt tgcgccagcc tgtccgcccg ctgtcggact
     2461 tcgtccggca tttcttcacc gccccgcgtc aacaactgca cgcgcgtata ttccgaagca
     2521 tattgcagca gcattcttgc ttcatttttg ggcagttttg acaagcccaa ccattcgtca
     2581 aacgtcattt ttatcccgtc tgccgctgat gcggcttttc tttccttatt ctttccggca
     2641 aacgtaccga tggtggcaac cgcaaatgcg gcataccaca aataaaatcc tgcaccgtag
     2701 cgcacaatat ccgatgtatt ccctgcttca tcgacgtata cggctttcac actgaaagcc
     2761 accaacgcca agccccaaag tgccgcatgg acaggcacga ccttcttccg caacgccagc
     2821 aaaacaatgg ccgccaacca aacataattc gcatagaccg cacaatacct gatatccaaa
     2881 gaagcaaata tcgaccccaa aatcaaaacg gtcaaaccct ccatgcttcc atgattgccc
     2941 aaataaaatg caacattgga taaagacgct atccacaggg caaccgacac cagcaacatc
     3001 actatgggaa aacttgattt ccgattctgt tcctgcatgg ttttatccta atgtaaaagg
     3061 ccgcctgaaa acctttcaga cggcatcgtg ccggattccg cgtcagattg cgctgccgcc
     3121 gacggtcagc ccggcatcga tacgcagggt cggctgcccc acgccgacgg ggacgctctg
     3181 cccttctttg ccgcacacgc cgacaccgct gtccaacgcg gtatcgttgc ctatcatcga
     3241 aacgtgtttc agcacttcgg gcccgttgcc gataatggtc gcgcctttga cggggtattg
     3301 cagcctgccg ccttccaccc accatgcttc ggacgcgctg aacacaaatt tgccgctggt
     3361 aatgtccacc tgtccgccgc caaagttgac ggcgtaaatg cctttgtcga tggacgcgat
     3421 gatttcttcc ggctcatagc tgccattttc cataaaggta ttggtcatgc gcggcatcgg
     3481 gacggaggcg tagctttcgc ggcggccgtt gccggtggat tgcgtacccg tcaggcgggc
     3541 attggtttcg tcctgcatat agccgactaa aatgccgtct tcaatcaata cggtgcggcg
     3601 ggtttcgttg ccttcgtcgt cgatgttgag cgaaccgcgc cggccggcaa tatcgccctg
     3661 atcgacgacg gtaacgcctt tggcggcgac gcgctcgcct attctgccgg aaaagacgct
     3721 ggtccccttg cggttgaaat cgccttccaa accgtgtccg accgcttcgt gcagcaacac
     3781 gcccggccag ccgttgccca aaacgacggt catttcgccg gcgggcgcgg ggcgggattc
     3841 gaggttggtg agtgcctgtt tgacggcggc atcgacaaac tgccgcacca agttttcatc
     3901 gaaataagcc aagtcgtagc gtccgccgcc gcccgcgctg ccctgttcgc ggcgttcgcc
     3961 ctgtttggcg atgacggtaa cgttcaggcg caccatcggg cggatgtcgg cggcgtgttt
     4021 gccgtccaga cgggcgaggt aaaccatatc gtattcgcag gtcaaaccgg ccatcacttg
     4081 cacgatgcgc ggatcggcgg ctttggcgat tgcttccact ttgttcaaca gcgcgacttt
     4141 ggcggcggaa tcgaggccgg caatggggtc ggacgcggaa caaaccggct tgccgcgcgt
     4201 ttcagacggc attttggcgg acaccttgcc gcctgccgcc ccaatcgcgc ggacggcgcg
     4261 ggcggaacgg tttatcgaat cgatgcacag gctgtcggcg taggcaaagg cggttttgtc
     4321 gcccgaaacg gcgcgcacgc ccacgccctg attgatttgg aagctgcccg atttgacgat
     4381 gccctcttcc aaatgccagc tttcataagc ggtgcgctgg cagtagatgt cggcgtaatc
     4441 gacgtggtgc gcgccgatga tgcacaggct tttggcgagc agttcggggg aaaggcggtt
     4501 ggcttcgagc agccgcgcct gtacggcgga ataggtcgga tgcatagtgt cggcgcataa
     4561 aaaatcaggg gcttgattat acggcatttg ttatatagtg gattaacaaa aaacagtacg
     4621 gtgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     4681 ttttgttaat ccactataga aatgcgccgt gccgcctgaa atgtaagatt tttgccaacg
     4741 ccccctgctt ttgtgtacac ttaaagctcc ttgtcggagt gccgccgccg ggcggctgag
     4801 attgcgaaag cagaatccgt agaacctgtc ggggtaatgc ctgcgtagga aacaaaccgt
     4861 caaatgcctt atcaggcttc cgttcccttt tccgcacttc cccgcccacc ttccttcttt
     4921 ttaaaggacg tcatatgtcg ggcaatgcct cctctccttc atcttccgcc gccatcgggc
     4981 tgatttggtt cggcgcggcg gtctcgattg ccgaaatcag cacgggtacg ctgcttgcgc
     5041 ctttgggctg gcaacgcggt ctggcggctc tacttttggg tcatgccgtc ggcggcgcgc
     5101 tgttttttgc ggcggcgtat atcggcgcac tgaccggacg cagctcgatg gaaagcgtgc
     5161 gcctgtcgtt cggcaaacgc ggttcagtgc tgttttccgt ggcgaatatg ctgcaactgg
     5221 ccggctggac agcggtgatg atttacgccg gcgcaacggt cagctccgct ttgggcaaag
     5281 tgttgtggga cggcgaatct tttgtctggt gggcattggc aaacggcgcg ctgattgtgc
     5341 tgtggctggt tttcggcgca cgcaaaacag gcgggctgaa aaccgtttcg atgctgctga
     5401 tgctgttggc ggttctgtgg ctgagtgccg aagtcttttc cacggcaggc agcaccgccg
     5461 cacaggtttc agacggcatg agtttcggaa cggcagtcga attgtccgcc gtcatgccgc
     5521 tttcctggct gccgctggcc gccgactaca cacgccaagc acgccgcccg tttgcggcaa
     5581 ccctgacggc aacgctcgcc tatacgctga cgggctgctg gatgtatgcc ttgggtttgg
     5641 cagcggcgtt gttcaccgga gaaaccgacg tggcaaaaat cctgctgggc gcaggtttgg
     5701 gtgcggcagg cattttggcg gtcgtcctgt cgaccgttac caccactttt ctcgatgcct
     5761 actccgccgg cgtaagtgcc aacaatattt ccgccaaact ttcggaaaca cccatcgccg
     5821 ttgccgtcgc cgttgtcggc acactgcttg ccgtcctcct gcccgttacc gaatatgaaa
     5881 acttcctgct gcttatcggc tcggtatttg cgccgatggc ggcggttttg attgccgact
     5941 ttttcgtctt gaaacggcgt gaggagattg aaggctttga ctttgccgga ctggttctgt
     6001 ggctggcggg cttcatcctc taccgcttcc tgctctcgtc cggctgggaa agcagcatcg
     6061 gtctgaccgc ccccgtaatg tctgccgttg ccattgccac cgtatcggta cgccttttct
     6121 ttaaaaaaac ccaatcttta caaaggaacc cgtcatgacc cgtatcgcca tcctcggcgg
     6181 cggcctctcg ggaaggctga ccgcactgca gcttgcagaa caaggttatc agattgcact
     6241 tttcgataaa ggctgccgcc ggggcgaaca cgccgccgcc tatgttgccg ccgccatgct
     6301 cgcgcctgcg gcggaagcgg tcgaagccac gcccgaagtg gtcaggctgg gcaggcagag
     6361 catcccgctt tggcgcggca tccgatgccg tctgaacacg cacacgatga tgcaggaaaa
     6421 cggcagcctg attgtgtggc acggacagga caagccatta tccagcgagt tcgtccgcca
     6481 tctcaaacgc ggcggcgtag cggatgacga aatcgtccgt tggcgcgccg acgacatcgc
     6541 cgaacgcgaa ccgcaactcg gcggacgttt ttcagacggc atctacctgc cgaccgaagg
     6601 ccagctcgac gggcggcaaa tattgtctgc acttgccgac gctttggacg aactgaacgt
     6661 cccttgccat tgggaacacg aatgtgcccc cgaagacctg caagcccaat acgactggct
     6721 gatcgactgc cgcggctacg gcgcaaaaac cgcgtggaat gcttctgccg aacacggcag
     6781 caccctgcgc ggcatacgcg gcgaagtggc gcgggtttac acacccgaaa tcacgctcaa
     6841 ccgccccgtg cgtctgctcc atccgcgtta tccgctctac atcgccccga aagaaaacca
     6901 cgtcttcgtc gtcggcgcga cccaaatcga aagcgaaagc caagcccctg ccagcgtgcg
     6961 ttccgggctg gaactcttat ccgcactcta tgccgtccat cctgccttcg gcgaagccga
     7021 catcctcgaa atcgctaccg gcctgcgccc cacgctcaac caccacaacc ccgaaatccg
     7081 ttacagcctc gcccgacgcc tgattgaaat caacggcctt ttccgccacg gcttcatgat
     7141 ttcccccgcc gtaaccgccg ccgccgtcag attggcagtg gcactggttg acggaaaaga
     7201 cgcgcccgaa cgtgatgaag aaagcggttt ggcgtatatc cgaagacaag attaaagccg
     7261 tctgaaagga accccatgac cttcccgccc ttgaaatccc cgctcaaatt ctacgccgtc
     7321 gtccccaccg ccgattgggt ggggcgcatg gtcaaagcag gtgccgacac ggtgcaactg
     7381 cgctgcaaga ccctgcacgg cgatgaattg aaacgcgaaa tcgcccgctg cgtcgccgcc
     7441 tgtcaaggca gccgtacgca gcttttcatc aacgaccact ggcgcgaggc aatcgaagca
     7501 ggcgcgtacg gcgtgcatct cggacaagaa gacatggaca ccgccgacct tgccgccatc
     7561 gccgccgccg gtttgcgctt gggtttgagt acgcactccg ttgccgaact cgaccgcgcc
     7621 ctgtccgtac accctagcta cgtcgccagc ggcgcgattt tcccgaccac gaccaagcaa
     7681 atgcccaccg ccccgcaagg cttggacaaa ctgtgcgagt atgtgaaaca ggcaggcggc
     7741 acgcccgtcg tcgccatcgg cggtatcgac ttgaacaacg cccgagccgt actcgccacc
     7801 ggcgtttcct cactcgccgc cgtccgcgcc gtgaccgaag cggaaaatcc cgaagcggtg
     7861 gttaaagcgt ttcaggcttt gtgggatgga taaaaccgaa agaagaaaat tcaatttgcc
     7921 gtgtcggcaa aacttagccc gttatcgcaa acatacttaa ctttaaatgt ggcatatcat
     7981 caaattccgt catttccgcg caagcgggaa tccgccttaa aacttgagaa accatcattt
     8041 gaaaaacagt ttccgaattt caaaaatgga ttcccgcctg cgcgggaatg acggcaaccg
     8101 gccggttgcg tatcaaaaaa taaagtaatt cggct
//
LOCUS       DAWFAD010000088         8073 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWFAD010000088 DAWFAD010000000
VERSION     DAWFAD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.088"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            96..404
                     /locus_tag="WGO49_001411"
     CDS             96..404
                     /locus_tag="WGO49_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306513.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            486..1517
                     /gene="ruvB"
                     /locus_tag="WGO49_001412"
     CDS             486..1517
                     /gene="ruvB"
                     /locus_tag="WGO49_001412"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ0306514.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1540..2268)
                     /gene="rpe"
                     /locus_tag="WGO49_001413"
     CDS             complement(1540..2268)
                     /gene="rpe"
                     /locus_tag="WGO49_001413"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ0306515.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            2414..2848
                     /locus_tag="WGO49_001414"
     CDS             2414..2848
                     /locus_tag="WGO49_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ0306516.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(2861..3724)
                     /locus_tag="WGO49_001415"
                     /pseudo
     CDS             complement(2861..3724)
                     /locus_tag="WGO49_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(3732..4349)
                     /locus_tag="WGO49_001416"
     CDS             complement(3732..4349)
                     /locus_tag="WGO49_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ0306517.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(4512..5126)
                     /locus_tag="WGO49_001417"
     CDS             complement(4512..5126)
                     /locus_tag="WGO49_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ0306518.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPGDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQIDLQEG"
     gene            5332..7104
                     /locus_tag="WGO49_001418"
     CDS             5332..7104
                     /locus_tag="WGO49_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ0306519.1"
                     /translation="MILPARFSDGISLSLRLKLLTGVWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGESDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFSDGISLTGDDRTLLQTLIRQLGVSLASAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENISFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQAQQGTTVSLTVASEESLK"
     gene            7101..7757
                     /locus_tag="WGO49_001419"
     CDS             7101..7757
                     /locus_tag="WGO49_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0306520.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
BASE COUNT         1983 a         2368 c         1889 g         1833 t
ORIGIN      
        1 agttttgagg acgataccca atccaagctt tgcaacagcc gccgccatat ccgctataat
       61 tcacgcttca gccatcctgc ccccgacata aaatcatgac cctgaaaacc gatttattgc
      121 ctaaaatcaa caacgaagat tatcaacgcc tcatcctcaa acacagtgcg gaatttagcg
      181 gtggcgaaat ccgcctgttg aacgaaatcc tcgaaaaatt caatttcgac gttgttcagg
      241 cgcaggcatt ggcgcaagcc gtcatgcagc aaatccgctt cgaccccaac gcctaccaca
      301 tcgacagcga cgacgaagac accaccggca tctgccccca ctgcatcaac ccgcctatgc
      361 cgcccctgcg cgactatctc gtttggcgcg aaacccgcgg ataaaacgct tttgaccgtt
      421 atcttttcaa tgccgtctga aacgccgccg accgttcgga cggcataccc gacaaaggga
      481 acactatgct gcaaaccgac aacctgaccg ccgcgcaacc gcaacgcatc gttgccgccc
      541 aaaccgcctc cgcacaggaa gaactgctcg aacgcgccct ccgccccaaa acgctggacg
      601 actacatcgg gcaagacaaa gccaaagaac agcttgccat tttcatccaa gccgccaaaa
      661 aacgcggcga agcactcgac cacgttttgc tcttcggccc gcccggactg ggcaaaacca
      721 cactggcgca catcatcgcc aaagaattgg gcgtaaattt gcgccaaacc agcggccccg
      781 tcctcgaacg cgcaggcgac ctcgccgccc ttttgaccaa ccttgatccg cacgatgtat
      841 tgttcatcga cgaaatccac cgcctcagcc ctgttgtcga agaaatcctc tatcccgcgc
      901 tcgaagacta ccggctcgac attatgatag gcgaaggacc cgccgcccgt tccgtcaaaa
      961 tcgacctgcc gcccttcacg ctcatcggcg cgaccacccg cgccggtatg ctgaccaatc
     1021 cgttgcgcga ccgcttcggc atcgtctccc gccttgagtt ttacgaaaac cgagacctta
     1081 ccaccatcgt cagccgttcg gcacaactgt tgcagctcga tatgtccgaa gaaggcgcgg
     1141 aagaaatcgc caaacgcagc cgcggtacgc cgcgcatcgc caaccgcctg ttgcgacgcg
     1201 tgcgcgattt cgccgacgtg aaaaacaacg gcacaatcga cggcggcatc gccgatgccg
     1261 ctttaagtat gctggacgtg gacgcgcagg ggctggacgt gatggacagg aaatttctcg
     1321 aagccgtttt gcacaaattc ggcggcggcc cggtcggttt ggacaatgtt gccgccgcca
     1381 tcggcgaatc tacagacacc atcgaagacg ttatcgaacc ctaccttatc caacaaggct
     1441 tcctgcaacg caccccgcgc ggcaggatgg cgaccgaacg cgcctacctg catttcgggc
     1501 tgcccgtcga aaaataacgc aatgccgtct gaaacagagc taattttcag acggcatttc
     1561 tatttcaatc attggcgcaa ggttcagcct gccgcttttt tccagttccg ccctcatcgc
     1621 atcaatcacc gccttatagt ctggtttgcc gaaaatcgca gaaccggcaa caaaggtatc
     1681 cgcaccagct cgggcaacgg cggcaatatt gtcggttttg atgccgccgt ccacttcgat
     1741 ggcgatgtgc cgcccgcttt gtgcttcgta ccgatccagc atcgcccgca cccggcggat
     1801 tttttcaagg gtgtgcggga tgaagctttg tccgccgaat ccggggttga ccgacatcag
     1861 caaaaccata tccagcctgt ccaatacgtt ttccaacaga tatacgggcg ttgccggatt
     1921 caacaccagc cccgcctgac agcccatatc acgaatcagg ctcaagctgc ggtcgatatg
     1981 gcggctcgct tcgggatgaa aagtgataat cgatgctcct gctttggcaa acgactgaat
     2041 caggtcgtca acgggttcga ccatcagatg cacatcgatc ggcacgcttg cataaggctt
     2101 caacgccgcg caaaccatag ggccgaaggt caggttcggc acataatggt tgtccatcac
     2161 gtcaaaatgg atcagatctg cacctgccgc aatgacgctt tccacctctt ctccgaggcg
     2221 ggcaaagtct gccgacaaaa tactgggtgc gatacggtag gcggtcatat tttttccttc
     2281 aatgccttta cgggcggact caacgcggga aagccccgaa acagcattat taacaaaagt
     2341 taactgccat aataccatct ttcacacggc aatcaagtat attgtccgaa atatctggat
     2401 taaaggaaaa gccgtgccac tgcctgctcc ctgccgtttt gccaaacctg ccgcctcttt
     2461 tttaagtatg gctttgcttt cctgtcagct ttcccacgcc gccacggctt atatcccccc
     2521 gaacgatttt caaccgaact gcgacatacg ccgactcggg ctgacccaaa gtcagcacaa
     2581 tgagctgcgt aaaatccgca ccgccttcaa aatggcgggc gacagggcgc gtttgaaggt
     2641 tatgcattcc gaacacagcc gccgccggtc tgtcgtcgaa atcatttcct cggatgtttt
     2701 taatcggaac gaggcgcgcg attatgtcga aagccgctat ttgtccggta tggattttgc
     2761 ggtggacgaa ttggaaatcc aacaccggtt cttccatatc ctcacaccgc aacagcagca
     2821 aatgtggctt tcttcctgcc tcaaataatc cccgaaacgc tcacaacgcc cgttgtttcg
     2881 gcagcctgcc cgcccagtcg caggcaaact gccacgcgga acgtcccgaa cggctgcccc
     2941 gcgtctgcgc ccactgcaat gccgccatct gtgcggtttc atcatacggc acgccgaaat
     3001 cttccagcca attttgcacc gccgccagat aatcgttttg atcgaacgga taaaaactga
     3061 gccacaatca gaatcggtcg gacagggata ttttttcttc caccgcttct ttctgatgga
     3121 tttccccccg catccccgtc gtaccggcat tctcgtcaaa atattcgggc atcaggtgcc
     3181 gtctgttgga agtcgcgtaa accaaaacgt tggcgcaacg ttgagacaga ccgccgtcta
     3241 acgcggtttt caatgcctta taggtttcat cgccgctttc aaacgacaaa tcgtcgcaaa
     3301 atacgataaa tttttccgga cgttccttca aaagcgtcaa caggtaaggc aggccgatta
     3361 aatcgctttt atcgacttcg atcaggcgca atcccttatc cgcatattcg tgtagcaggg
     3421 ctttgaccag cgaggatttg cctgttccgc gcgcgccgct catcaataca ttgttcgcgg
     3481 gtctgccgac aatgaactgt tcggtattac gcaccagcaa ttcggtttgc ctgccgactc
     3541 ccgccagcct taccaaggga aaggtgtgcg gatcgggcaa gtgttccaaa aaaccttttt
     3601 tgcccgcact ctgccagcgg aaggcaagcg cgttccaatc cgtatgcccg ggttcgggcg
     3661 gaagcacggc atccaaacgc cgcaaaacgg cataggcttt atcgagaaat tcgttcaatt
     3721 ccatctctgc ctcactttgc atatctttgc gccatcagcc gttcgacggt atcgacgatt
     3781 gcctgcgtat tcggatcgat ttcgatgttg atcctgtcgc cgacctttct gctgccgaac
     3841 agcgtccgtt ccaaagtttc gggaatcaga tggacattga aacggccgtc ttcgactttg
     3901 cctatggtca ggctgcaacc gtccaagccg acgaaccctt tggtcaggat atagggtttg
     3961 agttcatgcg ggagcgaaaa ccaaaccgtg cggttgaacc cgtcccgttc gatttcgaca
     4021 ataggcacgg ttgccataat gtgtccgctc atgacgtgtc cgccgatttc gtctccgaaa
     4081 cgcgccgccc gttcgatgtt gacgcaatcg ccttccttca gcagccccaa attggttttt
     4141 gccaaagttt ccgccattaa atcgaaactg acgcggtttc cttcgatttc ggtaatcgtc
     4201 aggcagcagc cgttattggc gaccgatgcg ccgcgttgca gattgtccgc cgcctcttgc
     4261 ggaagctcga cgacataagt gtgaaatgcc tccgacgggc ggtggattgc cgtcagtttt
     4321 cccaatcctt gaacaatgcc tgtaaacata atcctgtttc cctgtgtcgg taaaaatggt
     4381 gcaaattgta gcatctcccc gcgaaaaatg ccgtctgaaa tgccttcaga cagcattatg
     4441 cctccgattc gggcaaaaac cgcccggtat ggcttgacct ttcctttcca cgccggtcgg
     4501 cggtcttgcc cttatccctc ctgcaaatcg atttgcgtgt tcaagtcggc aaaatgcccg
     4561 tcaaactcga atctgaccgg ccgcgccctt tgctgctgca accagcttct taatgttttc
     4621 atacccgaaa acagataggg aatcgcgctt tgcagaatct gcgggcggat atacataatg
     4681 ttgtagtgca tcgttatcgg cgtttccaca taatacgcat tgcacaacgg tgtgcgtttc
     4741 gacacggttt caaacctcgc caccaaatcg cccggcagat acggcatatc gcacggcaca
     4801 accaaaagcc agtcggcagt cgccaactgc aaatcgttgg ctgcggtaca caatgccgaa
     4861 agcgggccga aatgctgcca ctgccgtgcg tccggaaaaa tatgcggact tcttcgagca
     4921 tattcttcca aattccggtt ggtgctgatg gcgatatggc tgacctgcgg cctgaccctg
     4981 tcgatgacat gatctatcag tgccttaccc cccaaaagag caagcccctt gtcctcgcct
     5041 cccatacggc tcgcctgacc gtcggccagt atcagggcaa aagttttcat tgcggatgtt
     5101 ctcttggaaa agttcgaggt tttcatgatt gcagtctgcc gttcccaatg actcaaaatg
     5161 ccgtctgaag cagacggcaa ataaattcat attatctgaa ttttatcata acatgattta
     5221 acactgaagc ggtacggatt atgttttcct taaccaattc tttctgagca gttgtatcta
     5281 atcccaaaga atgatattgt ttgcattatt tagaacaatt tttcgccgag catgatactg
     5341 ccagcccgtt tttcagacgg catcagcctt tccctacgcc tgaaactcct gaccggagtg
     5401 tgggtcgggt tggcggcatt gtctgtcgtt ttgacactgc tgctctcttt gcgtctggaa
     5461 aacgcggcct ccgtcatcga agaggcgggc aacttgagaa tgcaggcata ccgtctggca
     5521 tacatggcgg gtgaaggatc tccccgctcc caaatagaca atcaggttga cgaatttgaa
     5581 aaaagcctga atcgtatttc acaaagtgat gccatccatc cgctgattcc ttcggacacc
     5641 cctcttgctt atgatttgat acaatccatg ctgattatag attggcaggc acacatcctc
     5701 cccccgctcc agtcctaccg gcgaccgact caggtcgatc tctaccgctt tgccggaaac
     5761 atcgaactgt ttttgcaggc attggaaaat gccaacgaaa aaaacacatg gtggctcagg
     5821 cgttttcaat gggcaattat gttgatgacg ctggtgtcgt ctgtactgat gctgttttgg
     5881 caccagattt gggttatccg gccgctgcag gcgttaaggg aaggtgcgga acgcatcgga
     5941 cggaggtgtt tcgatattcc ggttcccgaa ggcggtacgc cggaattcaa acaggtcggg
     6001 cgttgtttca atcaaatggg cggcaggttg aaaattttat atgatgattt ggaaggacaa
     6061 gtcgccgagc agacacgcag tctcgaaaaa caaaatcaaa acctgaccct gctgtaccaa
     6121 actacacggg acctgcacca atcctacata ccgcaacagg ctgcagaaca ttttctaaac
     6181 cgtatcctgc ccgccgtagg agcagattcc ggcagagttt gtttggacgg cgaatccgat
     6241 gtttatgttt ccattcatca tgcggattgc ggcacagcag cttcggattt ggggaagtac
     6301 catgaggaaa tcttccccat tgagtaccag aacgaaacat tgggcaggct gttgctcagc
     6361 ttttcagacg gcatttccct caccggagac gaccgcacgc tgcttcaaac cctgattcgg
     6421 caattgggcg tatcgctcgc tagcgcgaag caggaagaag aaaaacgcct gcttgcagta
     6481 ttgcaggaac gcaacctgat tgcgcaagga ttacatgaca gcatcgcaca agcattaacg
     6541 ttcctaaacc tacaggtaca gatgctggaa accgcctttg ccgaaaacaa acgggaggaa
     6601 gccgcagaaa acatcagctt catcaaaaca ggcgtgcagg aatgttatga agatgtccgc
     6661 gaactgctgc tcaacttccg taccaaaatc agcaataaag aatttcccga agccgttgcc
     6721 gacctattcg cccgctttac gcaacaaacc gggataacgg tcgaaaccgc ctgggaaaac
     6781 ggttcgttcc tgccgcctca ggaagcgcag ctccaaatga tttttatcct gcaggaaagc
     6841 ctgtccaaca tccgcaaaca cgcccgcgcc acccatgtaa aattcaccct ttccgaacac
     6901 ggcggacgct ttaccatgac catccaagac aacggacaag gtttcgacac ggagaaaata
     6961 ggagaaccca cgggcagcca tgtcggactg cacatcatgc aggagcgtgc caaacgcatc
     7021 catgccgttt tagaaatccg ttcccaagct caacagggaa ccaccgtctc attgacggtt
     7081 gcatctgaag aaagcttgaa atgactatta aaattattct gatagacgac cataccctct
     7141 tccgcagcgg cattaaagcc cttttgtcgc gccaacacgg ttttgaagtc atcggcgaag
     7201 ccgcagacgg cctctcgggc atcaaaatga ttagtcggct gcaacccgat gtcgtcctgc
     7261 tcgatttgga tatgcccggc atgaacggac gcgaagcact ctcccaaatc atcagcatca
     7321 atccgcagca ggcagtgatt atgctgaccg tttccgaaga cagcgacgac ttgaccgaat
     7381 gtattcgcat cggcgcgcgc ggttacctgc tgaaaaacat caacgctgac tttctgctcg
     7441 aaagcatacg caaagccgcc gaaggcgata atgtattctc gcccgagatg accgccaaac
     7501 tcgttaaaag cctgatttcc ccccaacctg cccaagggac acaggcactc tcctcactta
     7561 cccctcgcga actggaaatc ttgggctatc tcgccgcagg acacagcaac aaaatcatcg
     7621 cccgccacct cgatcttgcc gaatccaccg tcaaagtcca cgttcaaaac ctgctccgca
     7681 aactcaacct cagcagccgg gtgcaggccg ctgtttacgc catccggcac aacgtccccc
     7741 aacccgtgct ggaataaggc gggttcagac ggcatattag gggttttaat ccccgtacgg
     7801 tcattcggat aacagaccaa gcatgtaagt ttatgccccc ataagtacgc ttggcatagc
     7861 agtaatattg ttcggtttag tgttttccgt ttgcccctat ctgatactgc aatatcagct
     7921 atgccgtctg aaaacgtatc atcatgatat tttcagacgg cataataaaa agcggaaata
     7981 ctaatgcggg gtaaaatgtt ccattccgaa tcccataaat atacaatggc ttatatcgtt
     8041 taagcatgtg taacccaccc tcatatcaat caa
//
LOCUS       DAWFAD010000089         7900 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWFAD010000089 DAWFAD010000000
VERSION     DAWFAD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.089"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            306..1619
                     /locus_tag="WGO49_001420"
     CDS             306..1619
                     /locus_tag="WGO49_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ0306521.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDLVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1842..2017
                     /locus_tag="WGO49_001421"
                     /pseudo
     CDS             1842..2017
                     /locus_tag="WGO49_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2013..3263
                     /locus_tag="WGO49_001422"
     CDS             2013..3263
                     /locus_tag="WGO49_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0306522.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVRGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGMEQQGTGLGLSIADTLATEYGRYL
                     ELSDSRRFGRGLLIRALLDKESLK"
     gene            complement(3297..4790)
                     /gene="rng"
                     /locus_tag="WGO49_001423"
     CDS             complement(3297..4790)
                     /gene="rng"
                     /locus_tag="WGO49_001423"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ0306523.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIDLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            5003..5260
                     /gene="grxC"
                     /locus_tag="WGO49_001424"
     CDS             5003..5260
                     /gene="grxC"
                     /locus_tag="WGO49_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ0306524.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5281..5724
                     /gene="secB"
                     /locus_tag="WGO49_001425"
     CDS             5281..5724
                     /gene="secB"
                     /locus_tag="WGO49_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ0306525.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5810..7852
                     /gene="recG"
                     /locus_tag="WGO49_001426"
     CDS             5810..7852
                     /gene="recG"
                     /locus_tag="WGO49_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ0306526.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTATETLEQLQTALPELNIG
                     LVHGRMKAAEKAEVMAQFAAGRLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLSELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQAREIAPILIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1665 a         2056 c         2324 g         1855 t
ORIGIN      
        1 cactataaaa tcccgcgtgt acggcacgcc ccgcgctccg caatgatagg ggcggtttcg
       61 tttgaatcgg cgcaccggga tgaatgcccc cgcgccgtcc cgataccggt ttgccggttg
      121 ttccgtatcg gttgccgccg tgaaatgacg gacggataca ggaatggcgg caggcgtaaa
      181 tgggaggaaa tgaccgattg acttgtttca aaataaaaaa gtagaatgca aggcttatcc
      241 cgcgtctgtg ggcggttttg cctgcacggt cggttttctg tttaaaataa aataaaggag
      301 aaatgatgct gacgtttatc ggactgctga ttatcggggt catcgtatgg ttgctgctga
      361 cggaaaaagt gtcgcccatc atcgcattaa tcttggtgcc gctgtttggg gcgttgctgg
      421 cggggtttga tgtatcccaa ttaaaagaat tttattcggg cggcaccaaa tcggtgatgc
      481 agattgtgat tatgtttatg ttttccattt tgttttttgg aatcatgaac gatgtggggc
      541 tgttccgtcc gatgataggc ggtttgatta agctgactcg gggtaatatc gtggcagtga
      601 gtgtggggac ggtcttggtg tcggtggtgg cgcagttgga cggggcgggt gcgacgacgt
      661 ttttattggt cgtccccgcc cttttgccgc tttacaagcg tctgcatatg aatccttacc
      721 tgctgttttt gctgctgact tccagtgcgg gattgattaa ccttctgccg tggggcgggc
      781 cgactgggcg ggttgcaagc gtgttgggcg cagatgtggg cgaattgtat aaacctttgt
      841 tgacggtgca aattatcggt gtagtgttta tccttgcgct gtccctgctt ttgggtgtgc
      901 gtgaaaaaag gcggattgtc cgggagttgg gcgcgttgcc cgccgtggcg gatttgataa
      961 agccggtgcc tttgtcggaa gaagaacaaa aattggcgcg tccgaaactg ttttggtgga
     1021 atgtcctgct gtttttggcg gcgatgagcc tgcttttttc gggcatcttc ccgccgggtt
     1081 atgtatttat gctggctgca acggcggcgt tgcttttgaa ttaccgcagc ccgcaggaac
     1141 agatggagcg gatttatgcc cacgccggcg gcgcggtgat gatggcgtcc attattttgg
     1201 cggcaggtac gtttttgggg attttgaagg gtgcggggat gttggacgcg atttccaaag
     1261 accttgtgca tatcctgccg gacgcgctgc tgccttatct gcatattgcc atcggtgtgt
     1321 tgggcattcc gcttgagttg gttttgagta cggacgctta ttatttcgga ctgtttccga
     1381 ttgtggagca gattacctcg caggcgggcg tggcgcctga agcagcaggt tatgcgatgt
     1441 tgatcggcag tatcgtcggc acttttgtta cgccgctttc gccggctttg tggatgggct
     1501 tgggtttggc gaaattgtcg atgggcaaac acatccgtta ttcgtttttc tgggcgtggg
     1561 gtttgtcgct ggcgatattg gccagttcga tagcggcagg aatcgtgcct ctgccgtaaa
     1621 cggcgaagcc gtctgaagcc cgaatcgttc ctgtggtctg tcgcaggctc tgcctgcctg
     1681 cccgtgtgtt gcaatcgcgc cccaatgccg tctgaaggca gttgccggcg cggttttttt
     1741 gtgctgcagt tttgaaaaat accggcaagc gtaaagaccg ctaaattgcg gctaattttg
     1801 gggtgttaga ataggcacgg tttattttga agggaaagtt gatgcgtgtt ttgctggtgg
     1861 aagacgatgc gatgattgcg gaggcggtgt cggcaagttt gaaagacggc ggctatgcgg
     1921 tggattgggt caaaaacggc gcgcggttgc cgctgcccag ccttatgact tgatgctgct
     1981 ggatttgggt ttgccggggc gggacggttt ggatgtttgt tccgctggca atgcttgcag
     2041 gtatgttttc ctactacgaa accttccacg aaacggaagc gttgcaggac gacctgctcc
     2101 gtcaggcggc attgtacgtc gcccccgatt ccaaacccga aactttgccc gagggcgacg
     2161 gcgatacgcg tattttggta cagatgccgc agcaggaaga ccctgttgtc agcctgcctg
     2221 cgcatctggc ggacggtctg cacacgcttc aggcggacgg ggacgacgat tattaccgcg
     2281 tctatatccg cacgaccgaa cagggacgga ttgccgttat gcaggaaaac gaataccgtg
     2341 aagatttggc ggcggatgcg gcacggcaaa gcgtgttgcc cctgctggcg gcactgccgc
     2401 tgatgatact gctgaccgtg tggattacgc acaaagccat gcgccccgtc cgcaaattgt
     2461 cgcaaagtct cgaacaacgc cgaatcaatg acctgtctgc tttgagtgtg gacaatattc
     2521 ccagtgaaat cagagggttc gtaaccgcca tcaacctgct tttgaaacgt gcggatgaag
     2581 acatccgcca ccgtcagcgg tttgtcgccg acgcggcaca cgaattgcgt acgccgatga
     2641 cagccctttc ccttcaggcg gaacggctca acaatatgtc gctcccaccc gatgcagcgc
     2701 ggcagcctgc cgttttgcag cagagcatca ggcgcaacaa acacctgctc gaacagcttt
     2761 tggcactggc gcgttcgcag tcggacgaaa cccctctgac gaaaacgaca ttcggtctgc
     2821 aaagccgttt ccgccaagtg ttgcaggaac tgatgccgct ggctttggaa aaacgtcagg
     2881 acatcggtgt ggcggtcaga ggcgatgtcg aagtgtctgc cgacgaaaca gaaatctata
     2941 cgctgattaa aacctttgcc gataacgcgg tacgttacac gccgcctgaa ggcaggatag
     3001 atttgggttt cacggacgaa gggaaatatc ttgccgtgtg ggtggaagat aacgggaacg
     3061 gcattcccga atccgaacgc gcccgcgtcc tcgatccgtt ttaccgtatt ttgggaatgg
     3121 agcagcaggg gacggggctg gggctgtcca tcgccgacac gcttgccaca gaatacggca
     3181 gatatttgga attgtccgac agccgccggt ttgggcgcgg gctgctgata cgcgccttgt
     3241 tggacaagga atccctgaaa tagacagatg ccgtctgaag gcttcagacg gcatttttac
     3301 atcaaaacga tgtcgtattg ttcctgcgtg taagcggttt cgactgccag agaaatcggt
     3361 ttgccgatga aatctatcag cattgccaag gattgcgatt cttcgtccaa aaacaaatcg
     3421 atgacgttgg gggcggcgag gatgcggaaa ctttcggcat cgtaacggcg cgcttcgcgg
     3481 acgatttcgc gctggatttc gtagcatacg gtttgcggcg ttttcagacg gcctctgcct
     3541 tggcaggaag ggcagggttc gcagaggact tggtttaagt tttcgcgcga gcgtttgcgc
     3601 gtcagctcga ccagtcccag gctggtaaaa ccgtgcaggg taacgcgggt acggtcgaag
     3661 gcgagggctt tggcaagctc ctgcaacacg gcttcgcggt ggctttcctg cgccatatcg
     3721 atgaagtcga tgatgatgat gccgccgagg ttgcgcagcc tcaattcgcg ggcgatggtg
     3781 tggcaggctt cgaggttggt gcggaaaatc gtttcatcga aattgcgtgc gccgacgaag
     3841 ccgccggtgt tcacgtctat cgtggtcatc gcttcggtgg attcgataat caggtagctg
     3901 ccgaagttga ggttgacgcg cggttgcagg gcgcggctga tttcctgctc gacgttgtgg
     3961 gtttcaaata gcgggcgttc gcctttgaac aattctatcc tgcccaatgc gccgtggacg
     4021 tattgttcgg caaaacgcgt catgcggccg tggtttacgg tggaatcgac gaggattttt
     4081 tgcgtgtcgc agccgaccat atcgcgcaac acgcgcaaac tcaagggcaa atcctgataa
     4141 agcagggttt ccggcggccg gattttcgcc tgttcttgga tgtgttccca cactttggtc
     4201 aggtagtcga tgtcggactg gagctgttcg tcggtggcgt tttcggcgtt ggtgcggatg
     4261 atgtagcccc ggcaggcatt ttccggcagg agcttgtcga ggcgttcgcg caggctgctg
     4321 cgttcggcat cgtcttcgat gcgttgggac acgccgatgt ggtcttcttg cggaagatgg
     4381 acgaggaaac gccccgccag cgagatttgg gtggaaagcc gcgcgccttt ggtgttgatc
     4441 gggtctttga tgacctgcac caaaacagac tgcccttcaa acagcatatg ttcgatgcgc
     4501 tgggtttctt cggggttgcg gcgttgttcg aggacatcga cgatgtgtaa aaacgccgcg
     4561 cgttccaagt cgatgtcgat aaacgcgctc tgcatcccag gcagcacgcg gcgcaccacg
     4621 cccaaataga tattgccgac taggctgtgt tcgctgttgc gctcgatgtg cagctcgcag
     4681 atattgtttt cctccaacac cgctacgcgc gtttcctgcg gcgtgatgtt gaccaatatc
     4741 gtttcgggcg ggcgcgcgat gtctttgggg atggggagtc ctgacaacat ggtttttcct
     4801 gaaaatgtaa taaaaatatt ttgcatccgt tccgccgcgc agcgcgggtg caaaaatatt
     4861 tttcacaatg tctatcatac tttaaaaaag aaactttgac acattccgca catgccgttt
     4921 gaacgtgcat ctgttccgca cttgccaaac aggggaaatg cccctatatt gattatcatt
     4981 gcaaaacttt cggaaaccca atatgcagac cgttaccatg tacacaggtc cgttttgtcc
     5041 ctactgcgcg atggcgaaaa ggctgctgca cgcggcaggt gtcggacata tcgacgaaat
     5101 ccgtgtcgat gcaagccccg aagcctttgc cgaaatgcag cagctttcgg gacagcgcag
     5161 cgtgccgcag attttcatcg gcgaaacgca cgtcggcgga tttaccgacc tctaccgcct
     5221 ccagcaggaa ggcgggctgg acggactgct gaacccttaa cccccaacta ggaaaacaaa
     5281 atgagcgaag aactgcaacc cgtattcagc atcgagcgac tgtatgtcaa agacttgtct
     5341 ttggaagtgc cgcacgcgcc gcaaatcttt ttggaacagg gcgagcccga agtggatatg
     5401 cgcgtttcca ccggcagcca aaagctggaa gacggctact acaacgtgga tgtaaccgtt
     5461 accgtaaccg ccaaattgga taacgagcgc acgatgtttt tgaacgaagt aacccaaagc
     5521 ggtattttcc gtttggaaaa catccccgaa gaagatgtgc agctgctgct gggcgtggcg
     5581 tgtccgaaca tcctcttccc ttacgcgcgc gaagccgttt ccggtacggt aacacgtgcc
     5641 ggcttcccgc ccgtcctgct cgcgccgatt aattttgagg cgatttacca acaacagcag
     5701 gaagccgaag ccgccggggc ttgattcctg cctgccgatg ccgtctgaat ccgtttcaga
     5761 cggtattttt gttttcggat aaaataggtg cgttcaaagg aatctgccta tgatgtcgcc
     5821 cgaaacccag aaacagctca aaatcaccga tgtttccgcc aagaagctcg acaaactcaa
     5881 cctccatacc gcgtgggatt tggtgttgca cctgccgctg cgttacgagg acgagacgca
     5941 cattatgccg attaaggacg cgccgattgg cgtgccgtgc caggtcgagg gcgaggttat
     6001 ccatcaggaa gtaacgttca aaccgcgcaa gcagttgatt gtccaaatcg ccgacggttc
     6061 cggcagcgtc ctttttctgc gcttcatcca cttttacgcc agccatcaga aacagacggc
     6121 ggtcggcaaa cgcatccgcg ccgtgggcga aatcaaacac ggcttttacg gcgacgagat
     6181 gattcatccc aaaatccgcg atgccgaggg cggcggtttg gcggaaagcc tcacgccggt
     6241 ttacccgacc gtaaacggtt tgaaccagcc cactttgcgc cgtattattc agacggcgtt
     6301 ggacgttacg ccgctgcacg acacgctgcc cgatgccctg ctgtgccgtc tgaagctgcc
     6361 acacctcgcc gaaagcctgc gtcttttgca ttcgccgccg ccgagtttca cgattcatca
     6421 gctttcagac ggcacgctgc ctgcatggca acggctcaaa ttcgatgaac ttttggcgca
     6481 acagctttcg atgcgcttgg cgcgacagaa gcgtatcggc ggcacggcgg cggcattggg
     6541 cggcgacggc acattgaccc aagccctgcg ccaagccctg ccgtttgccc tgaccgatgc
     6601 gcaagaaaaa gttgtttccg aaatctgccg cgatatggcg caaacctacc cgatgcaccg
     6661 cctgctgcag ggcgatgtcg gcagcggcaa aaccattgtc gccgccttgt ccgcgctgac
     6721 cgccatcgaa tccggtgcgc aggtggctgt aatggcgccc actgaaatcc ttgccgaaca
     6781 acattttatt aaatttaaac aatggctcga acctttgggc attgaagttg tccgcctttt
     6841 tggcagtttg cgtaaaaaag ccaaagacga agccaaagcc aaactcgccg acggcagcgt
     6901 caaaatcgcc gtcggcacgc acgccctgtt ttcagacggc gtggcgtttc acaatttggg
     6961 cttgaccatt gtggacgaac agcaccgttt cggcgttgcc caacgcctcg ccctcaaaaa
     7021 caaagggcgc gaagtccatc agctgatgat gtccgccacg cccatcccgc gcacgcttgc
     7081 gatgagtttt ttcgccgatt tggacgtgtc cgtcatcgac gaattgccgc ccgggcgcac
     7141 accgattaaa acgcgcctcg tcaacaacgt ccgccgcgcc gaagtcgaag gcttcgtcct
     7201 cggcacttgc cgaaaagggc ggcaggcgta ttgggtctgc ccattgattg aagagagcga
     7261 aaccctgcaa ctgcaaactg ctaccgaaac cctagagcag cttcagacgg ccttacccga
     7321 actcaacatc ggcttggtac acgggcgcat gaaagccgcc gaaaaagccg aagtcatggc
     7381 tcagtttgcc gcaggccgtc tgaacgtctt ggtcgccacc accgtcatcg aagtcggcgt
     7441 agatgtgccc aacgccgccc tgatggtgat cgaacacgcc gagcgcatgg gcttggcgca
     7501 gctgcaccaa ttacgcggtc gggtagggcg cggcgcggca gaaagcgtgt gcgttctcct
     7561 gtttgccgag ccattgagcg aactcgccaa agcgcggttg aaagtcatct acgaacacac
     7621 cgacggcttt gaaatcgccc gccaagacct caacatccgc ggccccggcg aatttctcgg
     7681 cgcgcgtcaa agcggcgtgc ctatgctgcg tttcgccaag ctcgaagaag acttacacct
     7741 tttggaacaa gcgcgcgaaa tcgccccgat actgattgag caaaaccccg aaatcgtcga
     7801 agcgcatttg gcaagatggc tttccggcag ggaagggtat ttgggcgtgt gagcaaaatg
     7861 ccgtctgaaa tattagaaaa aaaagtgtaa cggaaatctg
//
LOCUS       DAWFAD010000090         7747 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWFAD010000090 DAWFAD010000000
VERSION     DAWFAD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.090"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(13..1215)
                     /gene="trpB"
                     /locus_tag="WGO49_001427"
     CDS             complement(13..1215)
                     /gene="trpB"
                     /locus_tag="WGO49_001427"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ0306527.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(1400..2110)
                     /locus_tag="WGO49_001428"
     CDS             complement(1400..2110)
                     /locus_tag="WGO49_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ0306528.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFGDADYFKVPSNGEAAHS
                     EKAFDAEKIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECR
                     HICTTGGKATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKK
                     AAAYRAFFEMAGLCEKQL"
     gene            2307..3086
                     /gene="rsmA"
                     /locus_tag="WGO49_001429"
     CDS             2307..3086
                     /gene="rsmA"
                     /locus_tag="WGO49_001429"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ0306529.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            3259..3987
                     /locus_tag="WGO49_001430"
     CDS             3259..3987
                     /locus_tag="WGO49_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0306530.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            4060..4419
                     /locus_tag="WGO49_001431"
     CDS             4060..4419
                     /locus_tag="WGO49_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306531.1"
                     /translation="MYFVDRTAVVLKPTARFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDINKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEAELNITPVFDNMM"
     gene            4424..4864
                     /locus_tag="WGO49_001432"
     CDS             4424..4864
                     /locus_tag="WGO49_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ0306532.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LVASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKDKTAATLAGSSMVTPYALFLQ
                     WDTGGKTVSQCIIPDMTDKESYRRLKVWVLWRQPKKTAETDTPS"
     gene            4896..6170
                     /gene="folC"
                     /locus_tag="WGO49_001433"
     CDS             4896..6170
                     /gene="folC"
                     /locus_tag="WGO49_001433"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ0306533.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGFKVGTLTSPHLLRYNERIAVNAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNALAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPASLVAHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFHPQHSDDPTRNRNALPFPALRGAYQLSNAACTLTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHGIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            6183..7181
                     /gene="ftsN"
                     /locus_tag="WGO49_001434"
     CDS             6183..7181
                     /gene="ftsN"
                     /locus_tag="WGO49_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0306534.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETNGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKETKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            7174..7671
                     /locus_tag="WGO49_001435"
     CDS             7174..7671
                     /locus_tag="WGO49_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ0306535.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGILIITLLVMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHSGGTAE
                     TPEDD"
BASE COUNT         1942 a         2202 c         1996 g         1607 t
ORIGIN      
        1 agacggacga ggttacagtt taatcccttt gagcttcgcg acggtattga tgtctttgtc
       61 gccacgaccg gataggttga ccagaatcac ttggtctttg cccattttcg gcgcgttttt
      121 caccgcccaa gcaacggcgt ggctggattc gagcgcgggg atgatgccct cgaagcggca
      181 gagcaagtca aaggcttcga gtgcttcgtc gtctttggca acagtgtatt cgacgcgctt
      241 gatgtcgtgc agatggctgt gttccgggcc gatgccgggg taatccaagc ctgcggaaac
      301 agagtgcgta cccaaaacct gaccgttttc gtcctgcatc agatagctgc ggaaaccgtg
      361 caatacgcca atcggtgcgc ccgaagtaat cggcgcggcg tgatcggggg tgttcacgcc
      421 caaaccgcca gcctccacgc cgacgaggcg cacgttttct tcgccgatat acgggtggaa
      481 caaaccgatg gcgttcgatc cgccgcccac gcaggcaacg gcaacgtcgg gctgtctgcc
      541 gatggcttcc tgcatctgcg ctttagcttc gttgccaatc acgcattgga aatcgcgcac
      601 catttcggga tacggcgcgg ggccggcggc ggtaccgatg atgtagaacg tgtcgtccac
      661 gcgggcgacc cattcgcgca tggcttcgtt catcgcgtct ttcagcgtgc ggctgccgct
      721 ttctacacca accacgttcg cacccaataa tttcatacgg aacacgttgg gcatttggcg
      781 ttggatgtcg tccgcgccca tatacacgtc gcaagtcata ccgaagcgtg cggcaacggt
      841 ggcactcgcc acgccgtgct gacccgcgcc ggtttcggcg atgacgcgtt ttttacccat
      901 gcgttttgcc aacagtgcct gaccgatggt gttgttgact ttgtgcgcgc cggtgtggtt
      961 caagtcttcg cgcttcaacc agatttgcgc accgcccaga tgttcggaca accgcgcggc
     1021 gtggtaaacg gggctgggcc tgccgacata atgtttcaaa tcatggcgga acgcttccca
     1081 aaattcagga tcgtttttcg ctgccttata ggcatccgcc agctcttgca aagccggaat
     1141 cagggtctcg gacacgaaca gcccgccgtg ttcgccgaaa aagcccttct cgtcgggcgc
     1201 gtggtagttc ttcatcagat ttcgtccttg tgcttcaaat tttcagacgg caatttatag
     1261 tggattaaca aaaaccggta cggcgttgcc ccgccttgcc gtactatttg tactgtctgc
     1321 ggcttcgccg ccttgtcctg atttttgtta atccactata tttttcaaac atgccgtccg
     1381 aacgggaaat tgtcggcaat tataactgtt tttcacacaa acccgccatt tcgaaaaacg
     1441 cccgatacgc cgccgccttt ttcgccaaac tcaaaggata ggcacgcgaa gtcgaaggca
     1501 ggcgcgtcag cgtcaaatcc cgtccggcaa acggaaacgg cacggtttcg cccgttttcg
     1561 gcattttgat gccgccacct tgaatatcga gcaggatttc cgtcgccttg ccgcctgtcg
     1621 tacaaatatg gcggcactcg ggtatttttg ccaacaccgc cgccaaatcg acggtttcaa
     1681 ctacctttaa aaacttgtcg gacgcattgc cgtgctgacg taccgccttc aaaacggtcg
     1741 gacaggacgc aatcccccgt tcgtgcaaaa acgccttgat tttctcggca tcaaacgctt
     1801 tttcagagtg cgcagcctcc ccgttggacg gcactttgaa ataatccgca tcgccgaaaa
     1861 acaccagccc gtaaacgcgc cacatatcgt tttggaaatt cggataatga aactgcatcg
     1921 cgcgtttgtc ttccttgggc ggaaacgtcc ccatcatcat gaccgcagcc ttcggcggca
     1981 acacggaatc gaagggatgg gtttcgattt caagcagatt ttcagacacg ttcaatcctc
     2041 caacaggcaa aacggcagac agccggtttt tgcaccaaac cgccaaacac ggcaagcctt
     2101 caaacagcat tatcacgccc tgccgttttc ggttacaatg ccgacatcaa acggatactg
     2161 taaacacatt tatgtttcaa cacacaggac gacacataaa gcaccgccct atgtgccatc
     2221 ctgatttgga aggggttacg cccctcccaa atagagtctg attctaccgc cctaaagggc
     2281 ggggtttcaa ccgaaaagga aacacgatga aagaacacaa agcccgcaag cgtttcgggc
     2341 agaatttttt gcaggacacg cggattatca gcgatattgt caacgccgtg cgcccgcagg
     2401 cggatgatgt cgtgattgaa atcggtccgg gtttggcggc gattaccgaa cctttggcga
     2461 aaaagctgaa ccgcctgcac gttgtcgaaa tcgaccgcga catcgtatgc cgtctgaaaa
     2521 cgctgccgtt tgcggataaa ctggtgattc acgaaggcga tgtattgcag tttgatttca
     2581 acggcatcgc aggcaaaaag aaaatcgtcg gcaacctccc gtacaacatt tccacaccgc
     2641 ttttgttcaa gctggcggag gtggcggacg atgtcgtcga tatgcacttt atgctgcaaa
     2701 aagaagtggt cgagcgtatg gttgccgcgc cgaaaagcaa cgactacggc cgcttgggcg
     2761 tgatgctgca atattttttc gatatggaaa tgctgattga cgtaccgccc gaatcgttcg
     2821 accctgcgcc gaaagtggat tccgccgtcg tccgtatgat tccggtgaaa caccgcatcg
     2881 gcaaggcgga cgatttcgag catttcgcca aactggtgaa actcgccttc caccaacgcc
     2941 gcaaaaccat acgcaacaac ctgaaagagc ttgcaggcga cgatgatttg caggctgtcg
     3001 gcatcaatcc gcaagaccgt gccgaacaca tcgcgccgga aaaatacgta gcgttgagca
     3061 attatctggc gggcaaggcc gtctgaaaag ggagtaagcc gatgaagaaa tacctgcttt
     3121 tacccctgat actgccgctt gcagcctgcc tgaccaatcc gaacgaccgt ttttttgacg
     3181 gcagacgcta cacgcttcat catatggagc tcccgtaacg acaacctgca acggcaaatg
     3241 ccgtctgaaa gaaaagacat gatcaaattc aaaaacgtac acaaacattt caaagacctg
     3301 cacgtcatca acggcgtgaa cttggaaatc aaaaaaggcg aagtggtcgt cgtctgcggg
     3361 ccttcgggca gcggcaaatc cacactcatc cgcaccgtca accagcttga aagcatcgaa
     3421 agcggcgaga tttgggtgga cggtgtcaac gttgccgacc caaaaaccga tttgaacaaa
     3481 atccgcgaag aagtcggctt tgtgtttcaa agtttcaacc tctacccgca cctgaccgtg
     3541 ttggaaaaca ttaccctcgc accgatgaag gttaaggggc aaaatgcgga acaggcggag
     3601 aaaaaggcaa tggagctttt ggaacgcgtc ggactggcac acaaaaaaga cgccttccct
     3661 tcccaacttt ccggcggcca gcagcaacgc gtggcgattg cacgcggttt ggcgatggaa
     3721 ccgcgcgtaa tgttgtttga cgaaccgacc tccgcactcg accccgaaat ggtcggcgaa
     3781 gtgttgaaag tgatgaaaga cttggcagaa agcggcatga ccatgatgtg cgtaacccac
     3841 gaaatggggt ttgcccgcga agttgccgac cgcgtgattt tcgtcgataa agggcaaatc
     3901 ctcgaagacg aaacgccgga agcgtttttt accaacccga aacacgaacg cgccaagcag
     3961 ttcctgcaac aggtcatgac ccattgatgc tatgccgtct gaaaaccgaa cgttcagaca
     4021 gcatatcccg acaccgcaag atgattgaga aagaaaacca tgtattttgt tgaccgcacc
     4081 gccgtcgtcc tcaagccgac cgcgcgcttt ttggaatggc tcaaaagcac cgacgaaaat
     4141 atgcccgacc tgaccataga gcagcttcgt gccaactgtt ccgtcttcct cgttccgcag
     4201 ttcgacgaac cggaagccgt cgtttcctac ttcgacgaac gctaccgcca gattttcgag
     4261 gcggagctgg caggttggga catcaataag gataaatggc cgcaagatat ggggctgaag
     4321 gcgttttggg aatttttcga catcgaaatc cacgatatgg tgttggacat ggaagaagcg
     4381 gaattgaaca ttacccccgt gttcgacaac atgatgtaag gctgtgcgcg cctttcagac
     4441 ggcattgagg ccgtcccgca tcctgaacat cctgaccgta tcgctccact cggcttccct
     4501 tgccgtctgc ctgacctggt tttacggccg gatgatgtgg ttcggtttgg cggctttggt
     4561 ggcaagttac gcatactcgc tcaggataac gaatctgaaa caccgccatg ccataaccgc
     4621 cattaccata gaccgcgacg gacaggcgga aatcgtatcc ggcaaagaca aaacagcggc
     4681 aacacttgcg ggcagcagta tggttacgcc ttatgcctta ttcttacaat gggacaccgg
     4741 cggcaaaacc gtcagccaat gcatcatacc cgacatgacg gacaaagaat cctaccggcg
     4801 gctgaaagtc tgggtgcttt ggcggcagcc gaaaaaaacc gccgaaaccg atacaccaag
     4861 ctgatttcag acggcatttg aacacggaac actcaatgaa aacactgcaa gactggctct
     4921 cccatttgga aaccgcccac agcggtggct tgatcgatat gggcttggaa cgcgtaggcg
     4981 aagtgaaaaa acggatgaag ctcacgccgc aatgccccgt cgtcgttgtc gcgggaacaa
     5041 acggcaaagg ttcggtctgc gcctacctga cacagattta caaacaggca ggctttaaag
     5101 tcggtacgct gaccagcccg catttattgc gttacaacga acgcatcgcc gtcaacgccg
     5161 aacccgtttc ggacgatacg attattgcct ctttcgagcg catcgaagcg gcgcgcggcg
     5221 aaatatcgct gacttatttt gaattcaatg ccttggcggc cgtcgacatc ttcatgcgcg
     5281 aacaggttga cgtgatgata ttggaagtcg gcttgggcgg acgcttggac gcggtcaacg
     5341 catttgacgg cgattgcgcg gtggttacca gcgtggattt ggaccatcag gcgtttttgg
     5401 gcgatacggt cgagcaggtc ggctttgaaa aagcaggcgt gttccgcagc ggcaaacccg
     5461 ccatctgcgg tcaaaacccc gcgcccgcat cattggtcgc acacgccgaa gccataggcg
     5521 cgaaactgct gatggtgcag cgcgatttcg agtttcacgc gctggaaaac atccaatgga
     5581 actaccgctt ccatccgcaa cattcagacg accccacgcg caaccgcaat gccctgccct
     5641 tccccgcatt gcgtggcgca taccagcttt ccaacgccgc ctgcacgttg accgtattgg
     5701 aatgcttgaa cgacaaattg cctgtggaca tcggcgccat caaacgcgga ttgctgctgg
     5761 ttgaaaatcc cggccgcttc caagtcctgc ccggccgtcc gctgaccgtt ttggatgtcg
     5821 gacacaatcc ccacgctgcc cgcgccctgc gccgcagcct gattaatttg gcgtttgcgc
     5881 aaaaacgcac cgccgtattc agtatgctgt ccgacaaaga catagacggc gtgttggaaa
     5941 tcgttaaaga ccagtttgac gagtggtata tcgcaccgtt ggatgtaccg cgcggcatga
     6001 cgctcgagag cctgcaaaac aaactgcacg aacacggcat cgaaaacata cagacattcg
     6061 ccgccgtccg cgaagcatac cgggcggcag cctcaaaagc gggcgagaac gacagaatcg
     6121 tcgttttcgg ctcatttcat accgttgccg atgtcatgtc ggtactttaa aaggaaactt
     6181 ctatgtccga aaacaaacaa aacgaagtcc tgagcggtta cgaacaactc aaacggcgca
     6241 accgccgccg cctcgtaacg gcaagttgcc tggttgccgc ctcctgcatc ctgctggcag
     6301 ccgccctcag ttccggccct gccgaacaga ctgccggcga aacaaacggc gtagaaaaca
     6361 aagcggcagg tgcggcacaa acccctgcct tgaaatccgc cgccgacaaa ccgcaggact
     6421 tggcaggcga agacaagcct tctgccgccg acagcgaaat cagcgagcct gaaaacgtag
     6481 gcgcgccgct ggtgctgatt aacgaccgcc tcgaagacag caacatcaaa ggtttggaag
     6541 catccgagaa actgcaacag gcagaaaccg ccaaaaccgc accgaagcag gcaaaacaac
     6601 gcgctgccga aaaagtgccg gcaactgccg acagtacgga tacggtagcg gttgaaaaac
     6661 cgaaacgcac tgccgaaaca aaaccgcaaa aagcggaacg cactgccaaa gccaagccca
     6721 aagccaaaga aaccaaaacc gccgaaaaag ttgccgacaa accgaaaact gccgccgaaa
     6781 aaaccaaacc ggatacggca aaatccgaca gcgcggtaaa agaaacgaaa aaagccgaca
     6841 aggctgaaag caaaaaaaca gccgaaaaag accgttcgga cggcaaaaaa cacgaaacgg
     6901 cacaaaaaac cgacaaagcg gacaagacca aaaccgccga gaaggaaaaa tccggtaaaa
     6961 aagccgccat tcaggcaggt tatgccgaaa aagaacgcgc cttaagcctc cagcgcaaaa
     7021 tgaaagcggc gggtatcgat tcgaccatca ccgaaattat gaccgacaac ggcaaagttt
     7081 accgcgtcaa atcaagcaac tataaaaacg caagggatgc cgaacgcgat ttgaacaaat
     7141 tgcgcgtaca cggtatcgcc ggtcaggtaa cgaatgaata acctccccgt tgccgacctc
     7201 ctcgtcttcg ccatcatcgc cgcctgcatc gtgctatccg cgatgcgcgg cgtgattgcg
     7261 gaggcaggtt cgatggcggc atgggtggtt tccttctttt ttgccaaact ctttgccgca
     7321 cccttcgccg acctcgcctt tgcctcgttc caaccccgcc tgtttgcatt ggctctgtcg
     7381 ttcatttccc tgttcgtcat tgcctgtctg atccagaaaa tgctccgttc gctgctgacc
     7441 ggcgcagttt cggcggtcgg tttgggcttt gccaaccgca ttttgggcgg cgtattcggt
     7501 gcattgaaag gcattttgat tattaccctg ctggtcatgc tcgcttcaaa aaccgacctg
     7561 cccgataccg aagaatggcg gcaatcttac acactgccgt ttttcgtatc gctttccgaa
     7621 gccgtgttga accatagcgg cggcacggcg gaaactccgg aagacgattg attgccctgc
     7681 tggcgtttca gacggcattc tcttttatgc cgtctgaaat gttttttcct ttcctcagcc
     7741 atatcac
//
LOCUS       DAWFAD010000091         7733 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWFAD010000091 DAWFAD010000000
VERSION     DAWFAD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7733
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.091"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..1973
                     /locus_tag="WGO49_001436"
     CDS             <1..1973
                     /locus_tag="WGO49_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0306536.1"
                     /translation="KNYSLDQKNHIFVIQAPLANNKYIYTYKDVVIVLIAKKKIAFIN
                     FGDTDSFETFREDFIEDLNAISDKYEYKSKIGRPRDWKTIFKNFSLDELVSNDIQNFV
                     KNIEISSEREQRLSELIISLLTGSINDIEKIDGLNIQDNILDKIKKKIILFDSNQTNF
                     LYQNKNQKKIIIQGLSGTGKTELLLHKLKELYLESDNNRIFFTCNNKILAESLRNRIP
                     RFFNFMQVSQQIDWDNRLWCNHAWGSGKNKNSGLYSFICHFYGIPFLNYAQESSFSKI
                     CSMAIEQIKSNSDNLNKYAFDYILIDESQDFGESFFELCSLVTKHKIYIAGDIFQSIF
                     GEQKIYDHVDYLLNQCYRTDPKTLMFSHGLSMGLFESEKLQWLTDQQWEQYGYIFQKN
                     NNRYIFTREPIRRFEDLKDNDNSIHIENIKELNNSQISDKIVELIMDIKSKYPSVQPD
                     DIAIIFLEQNRRYNSEMLNYIRYTLEEKGIDWPINPAFESKQQLTGYLSITNFNHVKG
                     LEFPFVICISPYEVTADLRKRNAIYMAMTRSFLHSSLLMPVSTDNFFLYSRGLNEINS
                     TKRMTIEEPNEENKNRIREINIQFDGSHSFKDIVIEILDSFPKSSSITDKDREWCIQG
                     VLRSLGETYDKNAIKSHLKPLIDYIQGERNES"
     gene            1963..2631
                     /locus_tag="WGO49_001437"
     CDS             1963..2631
                     /locus_tag="WGO49_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306537.1"
                     /translation="MKADFFILDGNHLSRFKYIFQEAIRDKILVLRILFESIDYILHL
                     PKSDRYLNDYNKIGDYTIILSINKHSRIIFYSNQKIFSIVLPFTYFNDKKTYEFYFNS
                     ILINSGIVSYIKEFLDMLSKNGNTLVLDEIMEFYTNNPEKDSDKDNAIIILSDLILLE
                     DGYIRYDYDPEHENGNLHPLHHYDIFYQNKNTFKIGLKSAIGCKEFIELLDCTEERSF
                     LSKL"
     gene            complement(2745..2930)
                     /locus_tag="WGO49_001438"
     CDS             complement(2745..2930)
                     /locus_tag="WGO49_001438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306538.1"
                     /translation="MFDKQARFWIGEFDGEKECAAGNEVSAITRHRIFVFGLNVGEGT
                     VCCLGFNFVEVEVFRRH"
     gene            2953..3282
                     /locus_tag="WGO49_001439"
     CDS             2953..3282
                     /locus_tag="WGO49_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0306539.1"
                     /translation="MDVQKQYPFETVAVCVLPNHIHAIWTLPPDDADYSLLRRLIKTK
                     FSAYSPYTKNLSAGKQRQHERGIWQRRFYGHTVRDETDLQRCADYIHSNPIKQKMSRA
                     KTESCKK"
     gene            complement(3517..4407)
                     /gene="sucD"
                     /locus_tag="WGO49_001440"
     CDS             complement(3517..4407)
                     /gene="sucD"
                     /locus_tag="WGO49_001440"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ0306540.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPDECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKTKGLA"
     gene            complement(4418..5584)
                     /gene="sucC"
                     /locus_tag="WGO49_001441"
     CDS             complement(4418..5584)
                     /gene="sucC"
                     /locus_tag="WGO49_001441"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ0306541.1"
                     /translation="MNLHEYQAKELLAGYGLPVQGGILAHNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            complement(5684..5971)
                     /locus_tag="WGO49_001442"
     CDS             complement(5684..5971)
                     /locus_tag="WGO49_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306542.1"
                     /translation="MNKEIVGIFFIPAGIISMCMAVLWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            complement(6096..7529)
                     /gene="lpdA"
                     /locus_tag="WGO49_001443"
     CDS             complement(6096..7529)
                     /gene="lpdA"
                     /locus_tag="WGO49_001443"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0306543.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELVTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
BASE COUNT         2198 a         1659 c         1615 g         2261 t
ORIGIN      
        1 tgaagaatta ttcattagat cagaaaaatc atatatttgt gattcaagct cctcttgcaa
       61 ataacaaata tatatataca tataaagatg tagttattgt attaattgca aagaaaaaaa
      121 tagccttcat taattttgga gatacagatt cttttgaaac atttagagag gattttattg
      181 aagatttaaa tgctatctct gataaatatg aatataaaag taaaattggt agacctagag
      241 attggaaaac catatttaaa aatttctccc tagatgaatt agttagcaat gatatacaaa
      301 attttgttaa aaatattgaa atttcttccg agagagagca gcgattgagt gagttgatca
      361 tttctcttct tactggaagt attaatgata ttgaaaaaat cgatggtcta aatattcaag
      421 ataatattct tgataaaatt aaaaagaaaa ttattctttt tgactctaat caaacaaatt
      481 ttttgtacca aaataaaaat cagaaaaaaa ttattattca aggcttatca ggcacaggga
      541 aaacagaact attactacat aaattaaaag aattgtattt agagtcggat aacaatagaa
      601 tattttttac atgcaacaat aaaatattag cagaaagttt aagaaacaga atcccaagat
      661 tttttaactt tatgcaagta tcacaacaaa tcgactggga taatcgatta tggtgtaacc
      721 atgcgtgggg gtcaggcaag aataaaaatt ctggactata tagttttatt tgccattttt
      781 atggtattcc atttctgaac tatgcccaag agtcatcatt ctctaaaatt tgctccatgg
      841 caattgaaca aatcaaatca aattcagata atcttaacaa atatgcattc gattatatat
      901 taattgatga gagccaagat tttggtgaga gtttctttga attatgcagc ttagtgacta
      961 aacataagat ctatattgca ggagatatat tccaatctat ttttggagag caaaaaattt
     1021 atgatcatgt agactactta ttgaatcaat gttatagaac ggatcctaaa actttaatgt
     1081 tttctcatgg cttgagtatg gggctatttg aaagtgagaa attacaatgg ctaacagatc
     1141 aacaatggga acagtacgga tacattttcc aaaaaaataa taatagatat atatttacaa
     1201 gagaaccaat tcgacgattt gaagacttaa aagacaatga taatagcatt catatagaaa
     1261 atattaagga acttaataat tcccaaattt cagataaaat tgttgaatta attatggata
     1321 ttaagtctaa atacccaagc gttcagcctg atgacatagc cataattttc ttagagcaaa
     1381 atcgtagata caatagtgag atgcttaatt atattagata tacattagaa gaaaaaggga
     1441 tagattggcc tataaatcca gcctttgagt ctaaacaaca attaactgga tatttatcaa
     1501 tcacaaattt taatcatgtg aaaggattag aatttccttt tgtaatctgt atctcaccat
     1561 atgaagttac agccgactta cggaaaagaa acgctattta catggcaatg actagatcat
     1621 ttttacatag tagcctttta atgcctgtta gcactgataa cttcttttta tactctcgag
     1681 gactcaatga aattaattct actaaaagaa tgactattga agaaccaaat gaagaaaata
     1741 agaaccgaat tcgagaaatt aatatccaat ttgatggtag tcattcattt aaggatattg
     1801 ttatagaaat tctggattca tttcctaaat cgtcatctat tacagataaa gatagagaat
     1861 ggtgcattca aggagtttta cgttcattag gagaaaccta tgataagaat gcaattaaat
     1921 cacatctaaa acctctgatt gattatatcc agggtgagag aaatgaaagc tgattttttt
     1981 atattagatg gtaatcattt atcaagattt aaatatattt ttcaggaggc aatcagagat
     2041 aaaattttag tcttaagaat tttatttgaa tcaatagatt atattttaca tcttccgaaa
     2101 agtgatagat atctgaacga ttataataaa attggagact atacaattat attatctatt
     2161 aataagcatt cacgtattat tttttattcc aatcaaaaaa ttttttcaat agttctacca
     2221 tttacttatt ttaatgataa aaaaacatat gaattttatt ttaatagcat tcttattaat
     2281 tctggtatcg tttcgtatat taaagaattt ttagatatgt tatccaaaaa tggaaataca
     2341 ttagttttag atgaaataat ggaattttat acaaataatc ctgaaaaaga ttctgataag
     2401 gataatgcta ttattatatt aagcgatcta attttattgg aggatgggta tattcgatat
     2461 gattatgatc ctgagcatga aaatggtaat ttgcatcctc tacatcatta tgacattttt
     2521 tatcaaaaca aaaatacttt taaaattggt ttaaagtcag ccatcggatg caaggaattt
     2581 attgaattac tggattgcac cgaagaaaga agctttctta gcaaactgta gtctgcatac
     2641 tcaacccaca gcgtgcgtgg ctgcaccaca cacgcgggtt gcttgattgt cggtttgcca
     2701 ttttcaaatt tgctttcgtt gaagctagcg ctttcagacg gcctctaatg tcgtctgaaa
     2761 acctcaactt caacaaagtt aaaccctaaa caacaaaccg ttccttctcc aacgttcaaa
     2821 ccaaaaacaa aaatcctatg gcgcgttatc gcagaaactt cattgccggc ggcacattct
     2881 ttttcaccgt caaactcgcc gatccaaaat cgcgcctgct tgtcgaacat atcggcttcg
     2941 cgtgcggctt atatggatgt gcaaaaacaa tatccctttg aaaccgtcgc cgtatgcgtg
     3001 ctgccgaacc atattcacgc catttggacg ctgccgcccg acgatgcgga ttattcccta
     3061 ctccggcggc tgattaaaac caaattctcc gcatattccc cttatactaa aaacttatcc
     3121 gccggcaaac agcggcagca cgaacgcggt atttggcaac ggcgttttta cggacacacc
     3181 gtgcgcgacg aaacggattt gcagcgttgc gcggactaca tccattccaa tcctatcaaa
     3241 caaaaaatgt cgcgtgctaa aacagaatcc tgcaaaaaat aatatcagtc ttttcaaatg
     3301 cgatagttat cattccccaa cttcaaacaa ggcgtgtgca acctgactta cgggtttgct
     3361 cctgtttgac cgataaatat tctgcctgcc tcaaagggca aaaataatgt gggtatgttg
     3421 aggctgcctc tttttgaaat gacaaacaaa atgccgtctg aaacccgatt tccggtttca
     3481 gacggcattt gatgttcaat cagttgtcaa acctgattat gccaaacctt ttgttttcaa
     3541 cacttccagc atggtagtgc ccaactcggc agggctgcgg gtgtaagcga taccggcttt
     3601 ttcgaaagcg gcgaattttt cctcggcagt acctttaccg ccggaaataa tcgcaccggc
     3661 gtggcccata cgtttgcctt taggagcggt aacaccagca atgtaaccta caacaggttt
     3721 ggttacgttg gattggatgt attcggctgc ttcttcttcc gcagtaccgc cgatttcacc
     3781 gatcatgatg atggcgtcgg tatccgggtc ttcttggaag agtttcagtg cgtcgatttg
     3841 attcatacca ggaatcgggt cgccgccgat accgatacag gttgattgac ccaagcccag
     3901 tttggtggtt tgtgccacgg cttcgtaagt taatgtaccg gaacgggaaa taataccgat
     3961 gcggcccgga gtgtggatgt ggcccggcat aataccgatt ttgcactcat ccggagtaat
     4021 cacgcctggg cagttagggc cgaccaagcg ggtaccgttg ccgttggttt ccaaataacg
     4081 tttggctttg agcatgtcca gagtaggcac gccttcggta atcacaacga ccaagcctac
     4141 gcctgaatca acggcttcaa cgatagaatc caacacaaac ggagcgggaa cgtaaattac
     4201 agatgcatcc gcgccggttt ctttaaccgc ttctttcatg gtgttgaata caggcaggtt
     4261 caggtgggtt tgaccgcctt tgcccggggt aacgccgccg acaactttag tgccgtaagc
     4321 cagagcttgt tcggagtgga aagtaccgtt tttaccggtg aaaccttgaa ccaatacttt
     4381 ggtgtcttta ttaatcaata cgctcattct tttctcctta ggcgtttacg gctgcgacaa
     4441 ttttttcggc tgcgtcattc aggccgtctg cagaagtcag tttcagacct gattcgttca
     4501 ggattttcgc gccgagttcg gcgttgttgc cttccaaacg aacaacgaca ggaacgtcga
     4561 cgttgatttc tttaacggct gccacgatgg cttccgcaat catgtcgcaa cgtacgatac
     4621 cgccgaagat gttgatcaat acgccttgaa cggatttgtc ttccagaatc agtttgaacg
     4681 cttcaaccac gcggtctttg gttgcgccgc cgccaacgtc caagaagttg gcaggttggc
     4741 cgcctttcag tttgatgatg tccatagtgg ccatcgccaa accggcaccg ttcaccatac
     4801 agccgatgtt gccttccagg gcaacatagt tcaggtcaaa ttcagaagct ttcaactcgc
     4861 gttcgttttc ttgagatttg tcgcgcaatt cggcgatttt cggcaggcgg tagagcgcgt
     4921 tgctgtcgat gccgattttg ccgtccacgc aggcgagcgc gccgttttcg cgaactgcca
     4981 gcgggttgac ttcaaacagg gcgaagtcat tttcgacaaa cgctttgtac gcaccggtca
     5041 tcagtttgac gaactcgttg atttgtttgt ctttcaagcc cagttggaac gcaacttcgc
     5101 gagcttggca aggttgcagg ccgaccagcg gatcaacggt tactttgaag attttttcag
     5161 gagtttcggc agcaactttt tcgatttcca cgccgccttc ggtagaggcc atgaatgtaa
     5221 tgcggcgggt agaacggtca accactgcgc ccaagtacag ctcggtttga accggataca
     5281 tgtcttcgca aaccaaaacg ctgttgacag gttggccgtt ggcatcagtt tggtaagtta
     5341 ccaagttggt gccaatcagg ctttcagcca cttctttagc ttcttcgcgg cttttaacga
     5401 cttttacgcc gcccgcttta ccgcggccgc cggcgtgtac ttgtgctttg acaacagcga
     5461 atttgccgcc caatttgtcg taagctgcag cggcttcttc gccgttgtgt gccaaaatac
     5521 cgccttgtac gggcaaaccg tagccagcca gcagttcttt agcctgatac tcgtgtaaat
     5581 tcatggattt ctcctttaga ttagtggaat gcccccgagt ttgcctccaa atggcaagcc
     5641 cgtgcgtttc agactacctt tcggcggtcg gaaaatcgtc gtttcacttg ctgaaaggca
     5701 accacatccg cgccagcaga tacatggctg caccgcctcc cccgagaata aaaaagacga
     5761 tgcctttttt acgcgaattc gggcagagca gataaacggc aaggctgaaa ctgataaaca
     5821 acaatgccac gcgccaaacc aattctttat ctttgaaacg gttgagcgta taagtttcgg
     5881 tcatcatcac atacatctgc cacaatacgg ccatacacat gctgatgatg cccgccggta
     5941 taaagaaaat accgactatt tctttgttca taatgaatgt aaatgctgtt tgtttgtctg
     6001 acggaattat aaatcagaaa acgaagctct cggaacattc aatttgtcgg aggccttaag
     6061 gccgtctgaa aagtttcaga cggccttaat gtctatcaac cgtgcaaagc gcgtttgtcg
     6121 gccgccaatg cagcttcgtg aaccacttcg gacaaggttg ggtgggcatg gataatgcgg
     6181 gcgatgtctt cgctgctggc gaagaattcg agcgcagtca cgccttcggt aaccaattcg
     6241 ctgacaaccg gaccaatcat gtgtacgccc aagatgcggt cggttttggc atctgccaac
     6301 actttaaccg tacctttggc tttgcccatt gccaatgcgc gaccattcgc accaaaacct
     6361 gaagtacctt ttttgtactc cacgccttcg gctttgagct gctcttcggt tttacccacc
     6421 caagcgattt caggatcggt gtaaatcacg aacggtacgt tgttgaagtc gatatgcggt
     6481 ttttgaccgg caatgcgttc ggcaacggca acgccttcgt cgctggcttt gtgtgccaac
     6541 atcgggccgc gaaccacgtc gccgattgcc catacgttag gcaggttggt acggcattcg
     6601 ccatctactt tgataaagcc gcgctcgtct ttttccaagc ctacggcttc cgcgttcagg
     6661 cctttggtgt ttggaatacg gccgatggca acgatcagtt tgtcgaatac ttcggttttg
     6721 gcttcgccag cagcagtttc gtaagcaacg gaaacacctt tgccttcaga cttgatgtcg
     6781 ccgattttca cgcccaattc gatgctcaga ccttgctctt tggtgaagta tttgaaggct
     6841 tctttggcga tttgttggtc ggcggcagcc aggaaggtcg gcgcggcttc aagaatggta
     6901 acttccgcac ccacgcggtt ccatacggaa cccatttcca aaccaatcac accggaaccg
     6961 atcacgccga gtttggcagg tacttcggtc aggttcaatg cgccttcgtt gtccaatacg
     7021 ttcacattgt cgatagcgac ttgcggcaac ggacgcggta cggaaccggt cgctacgatg
     7081 acgtgtttgg cttcgataac ggttttctca cctttgttat cgacttcgat ttggtaagca
     7141 tcgccatttt tgccggcaaa ggaagccgta ccgaacaggc tggttacttt gtttttctgg
     7201 aacaggaatt tcacgccgcc ggtcagtttg gttacgatgg catctttgcg ctcaatcatt
     7261 ttggccacgt cgaattttac gtcgccgaca gtgataccgt gttcggcaaa ctcgtgttgc
     7321 gctgcgtgga aatgttcgct ggattgcaac agggctttag aagggataca acctacgttc
     7381 aagcaagtac cgcccaatgc aggggcattg cctgctttgt taacgcctgc atcgacacag
     7441 gcagttttga aacccagttg tgcggcacgg atggcggcaa cgtatccgcc cgggcctgca
     7501 ccaatcacta ctacatcata ttgagacatc gtgtcatcct ttgcttttag cagtatctca
     7561 aaataaaaac gatacttttc gtttcttttt tattttgaat gactgcgttt tgatggtttt
     7621 aagaaaaagc cgtctgaaag tccatatctg ccgattcatt cgaatcaaat gtttcagacg
     7681 gtctcaatcg tttgttgagc ctattgtagc acgatactac attacgctac atc
//
LOCUS       DAWFAD010000092         7732 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWFAD010000092 DAWFAD010000000
VERSION     DAWFAD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.092"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="WGO49_001444"
     CDS             complement(79..1425)
                     /gene="mnmE"
                     /locus_tag="WGO49_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ0306544.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1556..1711
                     /locus_tag="WGO49_001445"
     CDS             1556..1711
                     /locus_tag="WGO49_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306545.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1708..2139
                     /locus_tag="WGO49_001446"
     CDS             1708..2139
                     /locus_tag="WGO49_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ0306546.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2273..6637
                     /gene="app"
                     /locus_tag="WGO49_001447"
     CDS             2273..6637
                     /gene="app"
                     /locus_tag="WGO49_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ0306547.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFTYKIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFHD
                     NNNGTGKIDAKHEHNSLPNRLKTRTVQLFNVSLSETAQEPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTNTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     DLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSLLSVTPPASVESRFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAG
                     RDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPATTAFP
                     RARRARRDLPQLQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAEDR
                     RNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDD
                     GIGNSARLAHGAVFGQYGIDRFYIGISAGAGFSSGSLSDGIGSKIRRRVLHYGIQARY
                     RAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHI
                     SITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAK
                     GPQLEAQHSAGIKLGYRW"
     gene            complement(6746..7468)
                     /locus_tag="WGO49_001448"
     CDS             complement(6746..7468)
                     /locus_tag="WGO49_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0306548.1"
                     /translation="MFPDKYKLSLKENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASVSLEDIQTILNLKRAYQYVISHISNGEPVNISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGIDYFGTHEDIDFPDPPNMG"
BASE COUNT         2067 a         2154 c         1869 g         1642 t
ORIGIN      
        1 aaccaaacgg atcggattcc cgtttttatg ggataacgga atgttcagcc ggacaccgcc
       61 acgatgcttt cgatccgttt attttccgat acaaaacctc gaaaaaatca cgccgagcag
      121 gtcgtccgcc gtaaactcgc ccgtgatttc gccgcatgcg acctgcgcca agcgcaagtg
      181 ttcggcaaac agctcgattt gatggttgcc gcacaatgcc gccagcgaca attcttcctg
      241 cgctgctttg agtgcgttga cgtgccgcgt ccgcgccaaa aacagccctt cgccctcgcc
      301 ctgccatccg gcctcgcgca acaacgtccg tttcagcgcg tccaagccgt cgccggtttt
      361 cgccgacagc gcgatgacgg tttccgcgcc cgtaccgaac ccgcctgccg cgtgtgcgtg
      421 caaatcggat ttgctgtgga tttcgatgcg tttcaactcc ggcggcaacg cgtccaaaat
      481 cgcccgtgtc ttgtcgttca cgccttcgcg cggatcgacc aacaccagcg cgacatcggc
      541 ttcggatacg gctttgcggc tgcgttcgat gccgatgcgt tcgaccacgt cgtccgtctc
      601 gcgcaaacct gccgtatcga caatatgcac cggaacgccg tcaatcagga tacgttccct
      661 gaccgcgtcg cgcgtcgttc cggcaatatc ggtaacaatc gccacttcgt cgcccgccag
      721 cgcgttcaac aggctggatt tgccgacatt cggcgcgccg accaatacga cattcagacc
      781 ttcgcgcaaa atcgcgccct gctgcgcgtt ggcaagcaca tcatccacgg cgcggcgcaa
      841 gccgtccagt ttgccgcgtg cgtctgccgc ttcgagaaaa tcaatgtctt cctcgggaaa
      901 atctaacgtc gcttcgacca gcatccgcaa ggtaatcaag tcttcgacta gaccgtgtat
      961 ccgccgcgaa aaatcgcctt tgagcgagcg caacgccaga cgcgccgccg aacggctgga
     1021 tgcgtcaatc aaatccgcca cgccttccgc ctgtgccaag tccagtttgt cgtttaaaaa
     1081 cgcacgcttc gtaaactcgc ccggttcggc aaggcgcgcg cccaattcca aacagcggtt
     1141 cagcagcata tccatcacta ccggcccgcc gtgtccctga agctcgataa catcttcgcc
     1201 cgtaaaactt gccggtgcgg caaaaaaaag caaaagcccg ctgtcgattg cctgaccgtc
     1261 cgcgtccgta aaatcagcat aggttgccac gcgcggcttg ggcgttttcc cacacaaagc
     1321 ctctgccatc ggcagcaggt ttttccccga tatgcgtatc acgcccacgc cgccgcgccc
     1381 gggtgcggta gcgactgccg caatcgttgg aacgttatcc gacataaaac ccccgaaaat
     1441 tcaaaacagc cgcgattata gcaaatgccg tctgaagtcc gacggtttgg ctttcagacg
     1501 gcataaaacc gcaaaaatgc ttgataaatc cgtccgcctg acctaatata accatatgga
     1561 aaaacgaaac acatacgcct tcctgctcgg tataggctcg ctgctgggtc tgttccatcc
     1621 cgcaaaaacc gccatccgcc ccaatcccgc cgacgatctc aaaaacatcg gcggcgattt
     1681 tcaacgcgcc atagagaaag cgcgcaaatg accgaaaacg cacaggacaa ggcgcggcag
     1741 gctgtcgaaa ccgtcgtcaa atccccggag cttgtcgagc aaatcctgtc cgacgagtac
     1801 gtgcaaataa tgatagcccg gcgtttccat tcgggaccgt tgccgccgcc gtccgacttg
     1861 gcgcaataca acgacattat cagcaacggg gcagaccgca ttatggcaat ggcggaaaaa
     1921 gaacaagccg tccggcacga aaccatacgg caagaccaaa ccttcaacag gcgcgggcaa
     1981 ctgtacggct tcatcagcgt catcctgata ctgctttttg ccgtcttcct cgtatggagc
     2041 ggctaccccg caaccgccgc ctcccttgcc ggcggcacag tggttgcctt ggcgggtgct
     2101 ttcgtgattg gaagaagccg agaccaaggc aaaaattaat gacaaatcct agggcgtgct
     2161 tcatatccgc ccgaacgccg aaccgcacat ataggcacat cccgcgcgcc gcccggaagc
     2221 ggaagccgcg ccctcccaaa caaacccgaa tcccgtcaga taaggaaaaa taatgaaaac
     2281 aaccgacaaa cggacaaccg aaacacaccg caaagccccg aaaaccggcc gcatccgctt
     2341 ctcgcctgct tacttagcca tatgcctgtc gttcggcatt cttccccaag cttgggcggg
     2401 acacacttat ttcggcatca actaccaata ctatcgcgac tttgccgaaa ataaaggcaa
     2461 gtttgcagtc ggggcgaaag atattgaggt ttacaacaaa aaaggggagt tggtcggcaa
     2521 atcaatgaca aaagccccga tgattgattt ttctgtggtg tcgcgtaacg gcgtggcggc
     2581 attggtgggc gatcaatata ttgtgagcgt ggcacataac ggcggctata acaacgttga
     2641 ttttggtgcg gaaggaagca atcccgatca acatcgtttt acttataaaa ttgtgaaacg
     2701 gaataattat aaagcaggga ctaacggcca tccttatggc ggcgattatc atatgccgcg
     2761 tttacataaa tttgtaaccg atgcagaacc tgttgaaatg accagttata tggatgggcg
     2821 gaaatatatc gatcaaaata attaccctga ccgtgttcgt attggggcag gcaggcaata
     2881 ttggcgatct gatgaagatg agcccaataa ccgcgaaagt tcatatcata ttgcaagtgc
     2941 gtattcttgg ctcgttggtg gcaatacctt tgcacaaaat ggatcaggtg gtggcacagt
     3001 caacttaggt agtgaaaaaa ttaaacatag cccatatggt tttttaccaa caggaggctc
     3061 atttggcgac agtggctcac caatgtttat ctatgatgcc caaaagcaaa aatggttaat
     3121 taatggggta ttgcaaacgg gcaaccccta tataggaaaa agcaatggct tccagctggt
     3181 tcgtaaagat tggttctatg atgaaatctt tgctggagat acccattcag tattctacga
     3241 accacgtcaa aatgggaaat actcttttca cgacaataat aatggcacag gaaaaatcga
     3301 cgccaaacat gaacacaatt ctctgcctaa tagattaaaa acacgaaccg ttcaattgtt
     3361 taatgtttct ttatccgaga cagcacaaga acctgtttat catgctgcag gtggtgtcaa
     3421 cagttatcga cccagactga ataatggaga aaatatttcc tttattgacg aaggaaaagg
     3481 cgaattgata cttaccagta acatcaacca aggtgctgga ggattatatt tccaaggaga
     3541 ttttacggtc tcgcctgaaa acaacgaaac gtggcaaggc gcgggcgttc atatcagtga
     3601 agacagtacc gttacttgga aagtcaatgg cgtggcaaac gaccgcctgt ccaaaatcgg
     3661 caaaggcacg ctgcacgttc aagccaaagg ggaaaaccaa ggctcgatca gcgtgggcga
     3721 cggtaaagtt attttagatc aacaagcaga tgaaaataat aaaaaacaag cctttagtga
     3781 aatcggcttg gtcagcggca ggggtacggt gcaactgaat gccgataatc agttcaaccc
     3841 cgacaaactc tatttcggct ttcgcggcgg acgtttggat ttgaacgggc attcactttc
     3901 gttccaccgt attcaaaata ccgatgaagg ggcgatgatt gtcaaccaca atcaagacaa
     3961 agaatccacc gttaccatta caggcaataa agatattgct acaaccggca ataacaacag
     4021 cttggatagc aaaaaagaaa ttgcctacaa cggttggttt ggcgagaaag atacgaccaa
     4081 aacgaacggg cggctcaacc ttgtttacca gcccgctgca gaagaccgca ccctgctgct
     4141 ttccggcgga acaaatttaa acggcaacat cacgcaaaca aacggcaaac tgtttttcag
     4201 cggcagacca acaccgcacg cctacaatca tttaaacgac cattggtcgc aaaaagaggg
     4261 cattcctcgc ggggaaatcg tgtgggacaa cgactggatc aaccgcacat ttaaagcgga
     4321 aaacttccaa attaaaggcg gacaggcggt ggtttcccgc aatgttgcca aagtgaaagg
     4381 cgattggcat ttgagcaatc acgcccaagc agtttttggt gtcgcaccgc atcaaagcca
     4441 cacaatctgt acacgttcgg actggacggg tctgacaaat tgtgtcgaaa aaaccattac
     4501 cgacgataaa gtgattgctt cattgaccaa taccgacatc agcggcaatg tcagccttgc
     4561 cgatcacgct catttaaatc tcacagggct tgccacactc aacggcaatc ttagtgcaaa
     4621 tggcgataca cgttatacag tcagccacaa cgccacccaa aacggcgacc ttagcctcgt
     4681 gggcaatgcc caagcaacat ttaatcaagc cacattaaac ggcaacacat cggcttcggg
     4741 caatgcttca tttaatctaa gcgaccacgc cgtacaaaac ggcagtctga cgctttccgg
     4801 caacgctaag gcaaacgtaa gccattccgc actcaacggt aatgtctccc tagccgataa
     4861 ggcagtattc cattttgaaa gcagccgctt taccggacaa atcagcggca gcaaggatac
     4921 ggcattacac ttaaaagaca gcgaatggac gctgccgtca ggcacggaat taggcaattt
     4981 aaaccttgac aacgccacca ttacactcaa ttccgcctat cgccacgatg cggcaggggc
     5041 gcaaaccggc agtgcgacag atgcgccgcg ccgccgttcg cgccgttccc tattatccgt
     5101 tacacctccg gcttcggtag aatcccgttt caacacgctg acggtaaacg gcaaattgaa
     5161 cggtcaggga acattccgct ttatgtcgga actcttcggc taccgcagcg acaaattgaa
     5221 gctggcggaa agttccgaag gcacttacac cttggcggtc aacaataccg gcaacgaacc
     5281 tgcaagcctc gaacaattga cggtagtgga aggaaaagac aacaaaccgc tgtccgaaaa
     5341 ccttaatttc accctgcaaa acgaacacgt cgatgccggc gcgtggcgtt accaactcat
     5401 ccgcaaagac ggcgagttcc gcctgcataa tccggtcaaa gaacaagagc tttccgacaa
     5461 actcggcaag gcagaagcca aaaaacaggc ggaaaaagac aacgcgcaaa gccttgacgc
     5521 gctgattgcg gccgggcgcg atgccgtcga aaagacagaa agcgttgccg aaccggcccg
     5581 gcaggcaggc ggggaaaatg tcggcattat gcaggcggag gaagagaaaa aacgggtgca
     5641 ggcggataaa gacaccgcct tggcgaaaca gcgcgaagcg gaaacccggc cggctaccac
     5701 cgccttcccc cgcgcccgcc gcgcccgccg ggatttgccg caactgcaac cccaaccgca
     5761 gccccaaccg cagcgcgacc tgatcagccg ttatgccaat agcggtttga gtgaattttc
     5821 cgccacgctc aacagcgttt tcgccgtaca ggacgaatta gaccgcgtat ttgccgaaga
     5881 ccgccgcaac gccgtttgga caagcggcat ccgggacacc aaacactacc gttcgcaaga
     5941 tttccgcgcc taccgccaac aaaccgacct gcgccaaatc ggtatgcaga aaaacctcgg
     6001 cagcgggcgc gtcggcatcc tgttttcgca caaccggacc gaaaacacct tcgacgacgg
     6061 catcggcaac tcggcacggc ttgcccacgg cgccgttttc gggcaatacg gcatcgacag
     6121 gttctacatc ggcatcagcg cgggcgcggg ttttagcagc ggcagtcttt cagacggcat
     6181 cggaagcaaa atccgccgcc gcgtgctgca ttacggcatt caggcacgat accgcgccgg
     6241 tttcggcgga ttcggcatcg aaccgcacat cggcgcaacg cgctatttcg tccaaaaagc
     6301 ggattaccgc tacgaaaacg tcaatatcgc cacccccggc cttgcgttca accgctaccg
     6361 cgcgggcatt aaggcagatt attcattcaa accggcgcaa cacatttcca tcacgcctta
     6421 tttgagcctg tcctataccg atgccgcttc gggcaaagtc cgaacgcgcg tcaataccgc
     6481 cgtattggct caggatttcg gcaaaacccg cagtgcggaa tggggcgtaa acgccgaaat
     6541 caaaggtttc acgctgtccc tccacgctgc cgccgccaaa ggcccgcaac tggaagcgca
     6601 acacagcgcg ggcatcaaat taggctaccg ctggtaacac cggatatgcc gaaaggggtt
     6661 tgacgatgcc gccgtgcgct gtcaaaccct ttttctgccg ccgccttgtg cctgctccat
     6721 tgtctgataa ccgtcaaatt gatttttagc ccatgtttgg tgggtcgggg aaatctatat
     6781 cttcgtgcgt gccgaaatag tctatgcccg atatacaatt ttgatacaca aacttggaaa
     6841 tatcggtatc gtcgccggag cgatagaatg cggacagttt ttcattgaat tgcggcatct
     6901 gcatttcgga gatttccaag atgccgcagc cccccgccat catcagaccg ttggcaaata
     6961 aggttgccgt tcgtttgttg ccgtcccaaa aaacctgctg ccgcatacaa taaagcataa
     7021 aacggacggc tgcctccgtc gtcgaaccgc tttgcagtcc gatattttgt aacacgcggg
     7081 ccacttcaat ttccttcact ggattcgggg catgacggga accgtccaat agcgttacgc
     7141 cgaccgaacc ggtacggaaa tcaccgggtg ccaaagaatc gtccttggca acaatgttgt
     7201 tgattttttt aaggagtgaa atattgaccg gttcgccatt tgaaatatgc gaaatcacat
     7261 attgataggc acgtttcagg ttcaagatgg tttggatgtc ttcaagtgac acggaggcta
     7321 catttttgcc atggataatt tgttcggtct gcaataaggt cgtctgacaa ttctcaaaac
     7381 ggctgaggtt gtgaatttgg gcaaccaata ctttctttgc caaaaaaata ttttctttca
     7441 aactcaactt gtatttgtcg ggaaacacga taattcctta aataattaaa gcagtacgtt
     7501 aaatcatcat cttatcatca ctttcggggt ttatgttgca tgcgtccgtt ttctaatacg
     7561 gtttcccatc ccgcgaaacg aggcggaggc ctgtgcaaaa aacctgttca cgaaattttc
     7621 cgcattaacg atacgctgaa aatgcgctaa aaatgactgt gtttgaatat cggttgattt
     7681 catccgtttg tgtaacctcg cgccggtttt gtcatgattt tgcattatag tg
//
LOCUS       DAWFAD010000093         7726 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWFAD010000093 DAWFAD010000000
VERSION     DAWFAD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.093"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(69..485)
                     /gene="dksA"
                     /locus_tag="WGO49_001449"
     CDS             complement(69..485)
                     /gene="dksA"
                     /locus_tag="WGO49_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ0306549.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(632..1423)
                     /locus_tag="WGO49_001450"
     CDS             complement(632..1423)
                     /locus_tag="WGO49_001450"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ0306550.1"
                     /translation="MNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRERLQKEL
                     GVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTLSRYLGG
                     TRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1472..1879)
                     /locus_tag="WGO49_001451"
     CDS             complement(1472..1879)
                     /locus_tag="WGO49_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ0306551.1"
                     /translation="MEIRAIKYTATAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRAKEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1879..2565)
                     /locus_tag="WGO49_001452"
     CDS             complement(1879..2565)
                     /locus_tag="WGO49_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ0306552.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSGQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVALAVEVAKLPNLVVRG
                     LMCVAKANSSETELKTQFQTMQKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2685..3728
                     /locus_tag="WGO49_001453"
     CDS             2685..3728
                     /locus_tag="WGO49_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ0306553.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3891..5117
                     /locus_tag="WGO49_001454"
     CDS             3891..5117
                     /locus_tag="WGO49_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0306554.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5403..7553
                     /gene="yccS"
                     /locus_tag="WGO49_001455"
     CDS             5403..7553
                     /gene="yccS"
                     /locus_tag="WGO49_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ0306555.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQENVANAYEALGSYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDSNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETSSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVYQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLSETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1901 a         2565 c         1692 g         1568 t
ORIGIN      
        1 gactggtgcg cctttgtgaa tatactgtct gaaacttagg gcttcagacg gcattttgtt
       61 tgccgccatc agtcggcaaa ctgttttttc atcctctctc ggcgttcttg ggactcaaca
      121 gataaagtgg ctgtcgggcg tgccagcagc cgcttcagac cgataggctc tcccgtatcg
      181 gcacagaatc cataatcccc ttcatcaata ttgcggatgg tcgcctgtat tttactgaga
      241 agttttcgtt cccgatcgcg ggtacggagt tccaatgcgt actcttcttc ctgtgtggca
      301 cggtcggcag gatcgggggc tgattcgtgt tcttggagat gccctgtcgt agcggaagca
      361 ttttcgatga gttcgtcttg catttttacc agcaattcgc ggaaaaaagc caaatggtcg
      421 tcattcatat aatcgtcttc cggcccgctc caattcaaaa tatcttgttc tgtcagcttt
      481 gccataatgt atatcctctc ggttcaccga aaaaaacaaa gcaccacaaa gtggctttat
      541 tcctttgatt tttataaggc ttattgccta aggcgttttt gaaaacgcgc atcataacac
      601 gttttggatt ggttttttta ttttctttac attattgata ttgccgttcc atttcctgcg
      661 aacggcgcac acaggcacaa acgccctcac ttatggcttc ggcgacacga tgccgcctga
      721 aggcttccac ggcttcgtgg gttgtcccgc cttttgacgt tacatttttt tgaagcttct
      781 cgaaatcttc acccgtctgc tcggcaaggg caaccgctcc tttaaacgtt gccagactga
      841 gcgcgcgtgc ttctgccata tcaaaccctt gtcggatggc ggcattttgc aatgcgtcca
      901 gcagataaaa cacatatgcc ggcccgctgc cgctgatacc ggtaatgctg tgcatttgcg
      961 cttcatcttc caaccaaaca gtcaaaccga ctgatttcat gattcgatcg gcaatcctgc
     1021 ggtctgtttc cgatacttcc gcttcggcat acataccgga tacgcccagc ccgatttttc
     1081 cgggtgtatt cggcataacc cggacaatgc ggcgtgttcc cccgaggtaa cggctgagcg
     1141 tatcgaccgg caatccggct gcgacagaaa gcaccaatgc gccgttggtg cggatatttt
     1201 tgcacgcggc ttccatatcc tgcggcttga cggcaaggat taaaacatcg tcggaatgaa
     1261 gctccggcaa ggtttcagaa gtttcgaccc ccaactcttt ttgcaaacgt tcgcgctttt
     1321 ccgcaccccg gttggcaata tggatgcggt aaccgccttg tttgaccaat ccgcccgcaa
     1381 cggcagccgc catattgccg ccgccgagaa aataaacatt cattatttct accctattca
     1441 aaattacctg actaaattca ttcccaattt tttaccatcg gacgcgctcg gacagaccgt
     1501 tgcgccgcaa cccctgcttt ttcaaacaat caaaaccttc ctgtttgaac gtctcaatca
     1561 aaggtttgcc gtcacgcgta cgccaatcca acccgcaggc ttcaaacttc cctattttcc
     1621 tgtaaaagta agattgttgt gcggattcat attcctttgc cctgacggaa cgattgccgt
     1681 ctgaaagcgg tatctcgtag tcctctaaag acggcggact ctcgccgcca atatcccaaa
     1741 aatcgatggc ggccggtttt ctcggcttac accagccgga cagggacgaa cactcatacc
     1801 aacccgccaa ccggcagcct gcaaccgtaa aggcaagcaa cgcagccgtt gccgtatatt
     1861 ttattgcccg aatttccatc atcccctttt cccgaaaatc gcgctgccga tacggacgtg
     1921 tgtcgcaccg cactcaatgg cggcaggcat atcgtccgac atccccatag acagcacgtc
     1981 cgccttaacg ccagccgtat tgaggtcggc aagcaacttt tgcatggtct gaaactgggt
     2041 tttcaactcc gtttcactgc tgttggcttt ggcaacgcac atcagtccgc gcacgacaag
     2101 gttgggcaac ttcgccactt cgaccgcaag cgcgaccgct tcttcgggag caacaccgtg
     2161 ctttgccgcc tcgcctgcaa tattcacctc gatacacacc tgcaaaggcg gcattgaaga
     2221 aggacgctgc ccactcagcc ggacggcggt ctttagacgg catacggtat gcacccaatg
     2281 cgcgcgttcg gcgacaaact tggttttgtt ggactgcaca tcgccgatga cgtgccacac
     2341 gatgtcggtc aaatccgcca actcttccgt tttgccgtac cactcctgaa tatagttctc
     2401 gccgaaatca cgctgtccgg cggcgtaaac ttcgcggatg ccgtctgaag ggaaagtctt
     2461 accgacggca atcaggctga cggaatgcgg cttccttccc gcctgcagaa ccaattttcc
     2521 gatacggtcg gacacctccc gataacgttc ttgcaacacc gtcatagatt atcccctaat
     2581 taaaaatgat taaacagttg aaacccctcc agtcagggcg gtacaatcaa ggttgttaga
     2641 accattccaa ccaatcgaaa cattatacta aacagagccg cattatgcag attaccgact
     2701 tactcgcctt cggcgctaaa aacaaagcat ccgaccttca cctgagttcg ggcatatccc
     2761 ctatgattcg ggttcacggc gatatgcggc gcatcaacct tcccgaaatg agcgcggaag
     2821 aggtcggtaa tatggtaact tcggtgatga acgaccacca gcggaaaatc taccagcaaa
     2881 acttggaagt cgacttctcg ttcgaactgc ccaacgtcgc ccgattccgc gtcaacgcct
     2941 tcaacaccgg ccgcggtccc gccgccgtat tccgcaccat tcccagtacc gtcttatcgc
     3001 tggaagaatt gaaagccccg agcattttcc aaaaaatcgc agaatcgccg cgcggcatgg
     3061 tattggttac cggccctacc ggttcgggca aatcgaccac gcttgccgcg atgatcaact
     3121 acatcaacga aacccagccg gcacacatcc tgaccatcga agacccgatc gaattcgtcc
     3181 accaaagcaa aaaatccctg attaaccagc gcgagctgca ccagcacacc ctcagcttcg
     3241 ccaacgcgct gcgttccgca ttgcgcgaag accccgacgt tatccttgtc ggcgagatgc
     3301 gcgaccccga aaccatcggc ttggcactga ccgccgccga aaccggacac ttggttttcg
     3361 gcacgctgca cacgaccggc gcagcaaaaa ccgtcgaccg tatcgtggac gtattcccgg
     3421 cgggagaaaa agaaatggtg cgctctatgc tgtccgaatc gctgaccgcc gtcatctccc
     3481 aaaacctgct gaaaacgcac gacggcaacg gccgtgtcgc ctcgcacgaa atcctgattg
     3541 ccaaccccgc cgtccgcaac ctcatccgcg aaaacaaaat cacgcagatt aactccgtcc
     3601 tgcaaaccgg gcaggcgagc ggtatgcaga caatggacca atcgctgcaa tcgctggtgc
     3661 gccaagggct gatcgcaccg gaagtcgcac gcagacgcgc gcaaaacagc gaaagtatga
     3721 gtttctgaca cacaaccgcc ttccggccat accggcggga aaacaaggcg caaacacgcg
     3781 gggcgggacg cagcatcccg cccggctacc tttccgaaca agaagcgtcc gccttcctgt
     3841 tgaaacctgc cgccgcaaac tgcaaggctt aaaccgaaaa gaagctaacg atgaataccg
     3901 ataacctgca cgacatcttg gacgaaatgg ttcaagtgta ttctcaaaaa aaacaaagcc
     3961 gatccgaaac cccggccgaa atcggcgcac acttccaccc gctgctcgac cgcctgtgcg
     4021 aaaccgcaga agcacaaaac gcgtccgaca tccttatcag caaaggattc ccgccctcgt
     4081 tgaaaatcaa cagcgcatta accccgcagc cgcaaaaggc gctgacgggc gaggaaaccg
     4141 ccgccatcgc cgcatcgacg atgaacgccg aacaatcgga aatattccgg cgcgacggcg
     4201 aaatcaacta ctccgtccag tcgcgcagcg gcacgcgcta ccgcgccaac gcctaccaca
     4261 gccaaggcag cgcaggtttg gttttgcggc gcatcaacca cgtcatcccg caaatgcagg
     4321 aattgggcct gcccgaaaaa ctcaaagacc tcgccgtcgc accgcgcggg ctgctgatta
     4381 tcgtcgggcc taccggttcg ggcaaatcca ccacgatggc gactatgctc gaacaccgca
     4441 acaaaaccct gcccagccat atcgttacca tcgaagaccc gattgagttt atctacaaac
     4501 cgcgccgctg catctttacc cagcgcgaaa tcggcgtcga caccataaac tggcagacgg
     4561 cggtacaaaa cgctatgcgc cagtcccccg acgtggtctg catcggcgaa gtccgcagca
     4621 gggaaagtat ggaatacgcg atgcagctcg cccaaaccgg ccacctgtgc atttttacgc
     4681 tccacgccaa caccgcgccg cagtcgctcg aacgcatact caacttctac cccaaagaac
     4741 agcacaacca aatactgatc gacatcgccc tcaacctgac cggcatcatc tgccaacgcc
     4801 tcgccctcaa acaagacaaa acgggcagga cggcggttgt cgacttgctc atcaacacgc
     4861 ccgccatcca agacttcatc ctgaagggcg acctgatgaa catcagtaaa atcatggaaa
     4921 ccgccaaaac cgacggaatg cagacgatgg atcaaaacct tttcgaactg taccgtcacg
     4981 gcatcatcag ttacgaagaa gccctgcgcc agtccgtttc cgccaacaac ctgcgattgc
     5041 acatccaact gcacaaagaa ggcaaaacgc ccgaactcct ttacgacagg gtcaacggtc
     5101 tcaacctcat ttcctgatcc gcaaaaccca atgccgtctg aaaaccgcat ccccgttttc
     5161 agacggcatg attttatccg ccccattcat gtgctacact ttatagtgga ttaaatttat
     5221 agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagcacaa atagtacgga
     5281 accgattcac ttggtgcttg agcaccttag agaatcgttc tctttcagct aaggcgaggc
     5341 aacgccgtac tggtttttgt taatccacta tattcacttc ataacaataa accggtaaaa
     5401 ccatgaaaac cccactcctc aagcctctgc tcattacctc gcttcccgtt ttcgccagtg
     5461 tttttaccgc cgcctccatc gtctggcagc taggcgaacc caagctcgcc atgcccttcg
     5521 tactcggcat catcgccggc ggccttgtcg atttggacaa ccgcctgacc ggacggctga
     5581 aaaacatcat cgccaccgtc gccctgttta ccctctcctc gctcacggca caaagcaccc
     5641 tcggcacagg gctgcccttc atcctcgcca tgaccctgat gaccttcggc ttcaccattt
     5701 taggcgcggt cgggctgaaa taccgcacct tcgccttcgg cgcactcgcc gtcgccacct
     5761 acaccacact tacctacacc cccgaaacct actggctgac caaccccttc atgattttat
     5821 gcggcaccgt actgtacagc accgccatca tcctgttcca aatcatcctg ccccaccgcc
     5881 ccgttcaaga aaacgtcgcc aacgcctacg aagcgctcgg cagctacctc gaagctaaag
     5941 ccgacttttt cgatcccgac gaagccgaat ggataggcaa ccgccacatc gacctcgcca
     6001 tgagcaacac cggcgtcatc accgccttca accaatgccg ttccgccctg ttttaccgcc
     6061 ttcgcggcaa acaccgccac ccgcgcaccg ccaaaatgct gcgttactac tttgccgccc
     6121 aagacataca cgaacgcatc agctccgccc acgtcgatta tcaggaaatg tccgaaaaat
     6181 tcaaaaacac cgacatcatc ttccgcatcc accgcctgct cgaaatgcag ggacaagcct
     6241 gccgcaacac cgcccaagcc ctgcgcgcaa gcaaagacta cgtttacagc aaacgcctcg
     6301 gccgcgccat cgaaggctgc cgccaatcgc tgcgcctcct ttcagacagc aacgacagtc
     6361 ccgacatccg ccacctgcgc cgccttctcg acaacctcgg cagcgtcgac cagcagttcc
     6421 gccaactcca gcacaacggc ctgcaggcag aaaacgaccg catgggcgac acccgcatcg
     6481 ccgccctcga aaccagcagc ctcaaaaaca cctggcaggc aatccgtccg cagctaaacc
     6541 tcgaatcagg cgtattccgc catgccgtcc gcctgtccct cgtcgttgcc gccgcctgca
     6601 ccatcgtcga agccctcaac ctcaacctcg gctactggat actactgacc gcccttttcg
     6661 tctgccaacc caactacacc gccaccaaaa gccgcgtgta ccaacgtatc gccggcaccg
     6721 tactcggcgt aatcgtcggc tcgctcgtcc cctacttcac cccctccgtc gaaaccaaac
     6781 tctggatcgt catcgccagt accaccctct ttttcatgac ccgcacctac aaatacagtt
     6841 tctccacctt cttcatcacc attcaagccc tgaccagcct ctccctcgca gggttggacg
     6901 tatacgccgc catgcccgtg cgcatcatcg acaccattat cggcgcatcc cttgcctggg
     6961 cggcggtcag ctacctatgg cccgactgga aatacctcac gctcgaacgc accgccgccc
     7021 ttgccgtatg cagcaacggc gcctatctcg aaaaaatcac cgaacgcctc aaaagcggcg
     7081 aaaccggcga cgacgtcgaa taccgcgcca cccgccgccg cgcccacgaa cacaccgccg
     7141 ccctcagcag caccctttcc gacatgagca gcgaacccgc aaaattcgcc gacagcctgc
     7201 aacccggctt taccctgctc aaaaccggct acgccctgac cggctacatc tccgccctcg
     7261 gcgcataccg cagcgaaatg cacgaagaat gcagccccga ctttaccgca cagttccacc
     7321 tcgccgccga acacaccgcc cacatcttcc aacacctgtc cgaaaccgaa cccgacgact
     7381 ttcagacagc actggataca ctgcgcggcg aactcgacac cctccgcacc cacagcagcg
     7441 gaacacaaag ccacatcctc ctccaacagc tccaactcat cgctcggcag ctcgaaccct
     7501 actaccgcgc ctaccgacaa attccgcaca ggcagcccca aaacgcagcc tgaaaaagtt
     7561 tcggcatttt gtaagagagg gcagattgtc agacaggttg caagatagtg gataaagctt
     7621 tgcgcagggt aaatgcgtag caactgaacc gtcattccca cgaacctaca tcccgtcatt
     7681 cccacgaaaa cagaaaacca aaaacagaaa cctaaaatcc cgtcat
//
LOCUS       DAWFAD010000094         7677 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWFAD010000094 DAWFAD010000000
VERSION     DAWFAD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.094"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(171..1472)
                     /locus_tag="WGO49_001456"
     CDS             complement(171..1472)
                     /locus_tag="WGO49_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ0306556.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCTAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPNLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDGPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWWLAASRHTCPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(1462..2373)
                     /locus_tag="WGO49_001457"
     CDS             complement(1462..2373)
                     /locus_tag="WGO49_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0306557.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            2481..2879
                     /locus_tag="WGO49_001458"
     CDS             2481..2879
                     /locus_tag="WGO49_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0306558.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            2928..3533
                     /locus_tag="WGO49_001459"
     CDS             2928..3533
                     /locus_tag="WGO49_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0306559.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEIVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALCAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            3719..5830
                     /locus_tag="WGO49_001460"
     CDS             3719..5830
                     /locus_tag="WGO49_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0306560.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGAVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGYSRAFTASIDPYD
                     RASWPASGRLQPILTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNINPVHTLYASYNKGFAPYGGRGGYLSINTSSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDEQNDPYTWAVGGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAMLGWNHKNVNITFAAANLLNQKY
                     WRSDAMPGAPRTYTARVNYSF"
     gene            6033..6692
                     /locus_tag="WGO49_001461"
     CDS             6033..6692
                     /locus_tag="WGO49_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ0306561.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRCNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            6863..7558
                     /locus_tag="WGO49_001462"
     CDS             6863..7558
                     /locus_tag="WGO49_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0306562.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSSSSAPAAPAE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
BASE COUNT         1895 a         1947 c         2053 g         1782 t
ORIGIN      
        1 ttcatcgact tctatttttg ccggcgatgg gaacggtgtc ggcttgggtg gcggtgattt
       61 gggcatacct gatgattgaa agtgaaaaaa acggaagata ttgagtcatt cggacgcaat
      121 gccgtctgaa acggaagttc agacggcatt tgttttaggt tccgtaccgc ttagggaata
      181 ccggcgacag gataggcggg atagccgtgg gtatcgaccg aacaggcaaa ccgccaaggc
      241 gtgtggacgg tgtcggcgga caggtgggcg agttcgggaa tgtgccgttt gataaaagta
      301 ccgtccgggt cggtacggta tgaggcggcg gcaatgtcgg ggcaggtgtg ccgtgaggcg
      361 gcaagccacc agttgccttg gttgattgcc gcatcgaaat cggtcagctg ccgggcaaac
      421 catatctcgc cttcgcggcg ggggaggttt aaaacgtggc agaaaaaatc cgcgctcaag
      481 cgtctcaggg cggggtggag gctgccggtt ttgtgcaaac agcgcatcgc ggcatcgata
      541 atcggaatgc cggtctggcc ctgctgccaa agcgtcaggc gcagggtgtg ttccggattg
      601 ccgtctgaag ggccgtcatc cgtgtgctgc aaggcaagtt gaaggaaaaa atcgcggcgg
      661 atgatgttgt ccgcccacgc gttcagacgg cgttcgaggc tttcccgcgc gagcaggcgc
      721 ggcgagatgc agccggcact caaatacgcg cccatcagcg aagtgtgttt gcgcgagggg
      781 aaatccttta aaacggagta ggaatccgcc tgttcgagaa accgccgcca ctgccgccaa
      841 gccgccgttt cgccgctgtt ttgcggcagg aagatgccgt ctgaaagggc ggcaggcggc
      901 ggggcggaaa ggttttcggg gaagggttgg cggtatgccg cgaataggtt cggaccggcg
      961 gggggctgct tggaaaagcg gtcgagccat acttcgcggt agcggtcgaa atcggtgtgt
     1021 gccgtgccgc cgtcgggtat caggtcggtt ttgccgaaaa cggcgcggtc attgacgaag
     1081 gttaacgcga tgccgtgttt gtccaattca tgccaaaggg cgttgtcggc gagtttgtcg
     1141 gcaaaagtat gggattcgtc ggcgatgacg gcgcggatat tgaggcggac ggcgagccgg
     1201 acgagctcgg caggcgatgc cgccgtgtag agcgggatgc cgcgccctgc aagcccttgg
     1261 gcgagttcgg cggcggattg gcggtagaac gcggcgcggc gagggttgtc tgtttcggca
     1321 tcgtcaatcc aaataccgat aacgggcaaa ccttcggcaa cggcggtgca taaggcggcg
     1381 ttgtcgcgga tgcggaggtt ttggcggaac cagacgagcg tgtgtgcggc gcacgtgtcc
     1441 gcataaaggg ggcgggcggt ttcagacggc atttcggcag cctttcctgc tggcgatttt
     1501 ttcgttcaga aaatcgatga agctgcggac tttcgcgctt aagaacgccc tgtccgcata
     1561 aacggcattc aggcggtcgg tcgggacggt atattcgggc agcagcctca ccagcgagcc
     1621 gcagcgcaaa tcgtgttccg ccgcccaaag cggctgataa ccgatgcacg cgcccgcctt
     1681 aatcatttcg cgcatcatca gcgtgttgtc ggtacggatg acgggggtca gttcaagccg
     1741 gtattttttg ccgtccgatt tgcgggtgag gtcgagtttc tgctggttgg tgtaggtcgg
     1801 caggacggcg ggcagccccg ccacttcttc cggcgtttcc ggtacgccgt tgcgcctcag
     1861 gaaatcgggc gaggcgagca gggcaaattc gatttccgcc agcgggcgcg caatcagcga
     1921 cggggacagg gtttgggaaa cgcgcaacgc caaatccacg ccttcggcaa tcaaatcgac
     1981 gtggcggttg tccaaaatca gttctaatgt cacttcggga taacgttcgc ggtattccgc
     2041 cagccagttg catatctggc tgccggcaaa ccacagtggc atcgttacgc gcagcagccc
     2101 ctgcggtttt tccgtccccc cggcggcttt ttgcgcggca tcgtcgagcg tgtcgagcgc
     2161 gtaactgcat tgccggtagt attcttcccc ggcttcggtc aggctgaggt tgcggctgtt
     2221 gcggtgcagg agtttggctt ggacggtgtt ttccaagtgg ctgacgtgtt tgcttgccat
     2281 tgcggtggag atgccgagcg cgtcggcggc gcgggtgaag ccgccgcttt ggacgacttg
     2341 gcggaaaacc ttgaggctga acagggtgtc catattttct tgtgtggaaa agttgtatca
     2401 ataaaagcag tatatatttg aaaaggggaa acatctatac tctaccgcct gaaatgaaga
     2461 caaatatcaa aggagctttt atgtccgatt gctgcaaccg tatccaaccg gttttgcttt
     2521 ctgttttgcg tatcgtaacc gcttacctgt ttttgttgca cggtacgtcg aaaatcttcg
     2581 ccttccccat tgaaatgggc agcggttcgc ccggcgggct gttgctgctt gccggtattt
     2641 tagaaattgt cggcggcatt ttgctggtgt tgggcctgtt tgcgcgccct gccgcgtttg
     2701 ttttgtccgg ccagatggcg gttgcctatt ttatggcgca cgcttccgga aatgctttgt
     2761 tcccgattgc caacggcggc gagtccgcag tgctgttctg cttcgtattc ctctatatcg
     2821 cggcggcggg cggcggagca tggtcgctgg acaggctgtt tttcaagcgt aaagcctgaa
     2881 tcagactgcc taaagtgtat tttgttgaat gtttttgagg aaaagaaatg acccgtcaat
     2941 ctctgcaaca ggctgccgaa agccgccgtt ccatttattc gttaaataaa aatctgcccg
     3001 tcggcaaaga tgaaatcgtc caaatcgtcg aacacgccgt tttgcacaca ccttcttcgt
     3061 tcaattccca atctgcccgt gtggtcgtgc tgtttggcga agagcatgat aaggtgtggc
     3121 aatttgtcga agacgcgctg tgtgccgtcg tgcctgccga cagttttgaa ccgaccgcgc
     3181 aaaaattgaa cctgtttaag gcgggtgcgg caactatttt gttttatgaa gatcaaaatg
     3241 tcgtcaaagg tttgcaggag cagttccctg cttatgccgc caactttccc gtttgggcgg
     3301 accaggcgaa cgcgatggtg cagtatgccg tttggacgac acttgccgcg gtcggcgtag
     3361 gtgcaaacct gcaacattac aatcccttgc ccgatgcggc gattgccaaa gcgtggaata
     3421 tccccgaaaa ctggttgttg cgcgcacaaa tggttatcgg cggtattgaa ggggcggcag
     3481 gtgaaaagac ctttgaacca gttgcagaac gtttgaaagt gttcggcgca taatttcgcg
     3541 gtcaaaaaaa tgccgtctga accctgttca gacggcattt ttcagtatca ggcggcgagt
     3601 tttccgcatt ctgagacctt tgtttacaaa tatcatgttc aatatagtta aaagaaatta
     3661 ttctcatttc ctccgtgagg caatataatt cggttgtttt gttaaattga gtataaaaat
     3721 gaaaatatca tttcatttag ctttattacc cacgctgatt attgcttcct tccctgttgc
     3781 tgccgccgat acgcaggaca atggtgaaca ttacaccgcc actctgccca ccgtttccgt
     3841 ggtcggacag tccgacacca gcgtactcaa aggctacatc aactacgacg aagccgccgt
     3901 tacccgcaac ggacagctca tcaaagaaac gccgcaaacc atcgatacgc tcaatatcca
     3961 gaaaaacaaa aattacggta cgaacgattt gagttccatc ctcgaaggca atgccggcat
     4021 cgacgctgcc tacgatatgc gcggcgaaag cattttcctg cgcggttttc aagccgacgc
     4081 atccgatatt taccgcgacg gcgtgcgcga aagcggacaa gtgcgccgca gtactgccaa
     4141 catcgagcgc gtggaaatcc tgaaaggccc gtcttccgtg ctttacggcc gcaccaacgg
     4201 cggcggcgtc atcaacatgg tcagcaaata cgccaacttc aaacaaagcc gcaacatcgg
     4261 tgcggtttac ggttcgtggg caaaccgcag cctgaatatg gacattaacg aagtgttgaa
     4321 caaaaacgtc gccatccgtc tcaccggcga agtcgggcgc gccaattcgt tccgcagcgg
     4381 catagacagc aaaaatgtca tggtttcgcc cagcattacc gtcaaactcg acaacggctt
     4441 gaagtggacg gggcaataca cctacgacaa tgtggagcgc acgcccgacc gcagtccgac
     4501 caagtccgtg tacgaccgct tcggactgcc ttaccgcatg gggttcgccc accggaacga
     4561 ttttgtcaaa gacaagctgc aagtttggcg ctccgacctt gaatacgcct tcaacgacaa
     4621 atggcgtgcc caatggcagc tcgcccaccg cacggcggcg caggattttg atcatttcta
     4681 tgcaggcagc gaaaatggca acttaatcaa acgtaactac gcctggcagc agaccgacaa
     4741 caaaaccctg tcgtccaatt tcacgctaaa cggcgactac accatcggcc gttttgaaaa
     4801 ccacttgacc gtaggcatgg attacagccg cgaacaccgc aacccgacct taggttacag
     4861 ccgcgccttt actgcttcca tcgatccata cgaccgagca agctggccgg cttcgggcag
     4921 attgcagcct atcctcaccc aaaaccgcca caaagccgac tcctacggca tctttgtgca
     4981 aaacatcttc tccgccacgc ccgatttgaa attcgtcctc ggcggtcgtt acgacaaata
     5041 cacctttaat tccgaaaaca aactcaccgg cagcagccgc caatacagcg gacactcgtt
     5101 cagccccaac atcggcgcag tgtggaacat caatcccgtc cacacacttt acgcctcgta
     5161 taataaaggc ttcgcgcctt atggcggacg cggcggctat ttgagcatca acacgtcgtc
     5221 ttccgccgtg ttcaacgccg accccgagta cacccgccaa tacgaaaccg gtgtgaaaag
     5281 cagttggctg gacgaccgcc tcagcactac gttgtctgcc taccaaatcg aacgcttcaa
     5341 tatccgctac cgccccgacg agcaaaatga tccctacact tgggcagtcg gcggcaaaca
     5401 ccgttcgcgc ggcgtggaat tgtccgccat cgggcaaatc atccccaaaa aactctatct
     5461 gcgcggttcg ttgggcgtga tgcaggcgaa agtcgttgaa gacaaagaaa atcccgaccg
     5521 agtgggcatc catttgaata acaccagcaa cgttaccggc aacctgtttt tccgttatac
     5581 cccgaccgaa aacctctacg gcgaaatcgg cgtaaccggt acaggcaaac gctacggtta
     5641 caactcaaga aataaagaag tgactacgct tccaggcttt gcccgagttg atgccatgct
     5701 tggctggaac cataaaaatg ttaacattac ctttgccgca gccaatctgc tcaatcaaaa
     5761 atattggcgt tcggatgcca tgcccggcgc gccgcgcact tatacggcgc gggttaatta
     5821 cagtttctaa tcccgttcgt gcaacagaat gccgtctgaa aatcctgata atccgtaaat
     5881 tcagcacacg aactttttga caggctttat ctacatcagt ataatccaaa aagaaatagc
     5941 gcccttggca gtacgcatta cccattaaat catagcgggt atgccgatcc caagggcttt
     6001 tttatatctt tgacaaatat aggggaagca atatgaaaac cgccattttg attgacggtg
     6061 cattttttat taagcgggta cggtcattag gcctgtctca aaatccacac gatgcaaaaa
     6121 gaattgctga tttggctttc cgatattcga tgctgcatct gaaagataaa atcaacggca
     6181 cagatcaaat ggatgattta tatagaattt tcttttacga ctgcgtgccg tttgaaaaga
     6241 aaatgcacaa tcccgtatct catcgtgcca tagatttttc aaagactccg gaagccatat
     6301 ttcgttgcaa tctgcatacc gaattgaaga agaagcgtaa attagcgtta cgtttaggca
     6361 agctgtcgga caatacagca tggatattaa aaccccaagt catgaaaaat cttctgaaaa
     6421 acccgtcaac tcaaattacg gaaaacgatg tcgtgctcga tgttaaacaa aaaggtgtag
     6481 atatgcgtat aggcttggat atttcatcta ttaccttaaa aaaacaagcc gataaaatca
     6541 tcttgttttc tggtgattcc gattttgtcc cagcagccaa attagccaga cgggaaggta
     6601 tcgattttat tcttgatcct atgtggaatc atgtcccacc tgacttaatg gaacacatcg
     6661 acggattgaa gacaaaatgt ccgaaaccgt aaattactag aacaaaggcc gtctgaaaat
     6721 aaggctaatt atctaagcca tatttcagac ggcctttata aaggctgaaa taaaactaaa
     6781 cacatttttt cactcaaatc gaacacgcct tcaataaaat gccataaaat ccgcacatta
     6841 atctgacaca caagagatac ctatgaaact gaaaacctta gctttgactt cattgaccct
     6901 gttggcattg gccgcttgta gcaaacaggc tgaaaccagt gttccggcag acagcgccca
     6961 aagcagctca tctgctccgg cagcccctgc tgagttgaac gaaggtgtga actacactgt
     7021 attgtctacg cctattccgc aacagcaggc cggtaaaatc gaagtattgg aatttttcgg
     7081 ctacttctgc ccgcattgcg cccatcttga gccggtcttg agcgagcaca tcaaaacgtt
     7141 taaagacgat acctatatgc gccgggagca tgtcgtgtgg ggtgatgaaa tgaaaccttt
     7201 ggcacgtttg gcggccgcag tggaaatggc cggtgaatca gataaagcca acagccatat
     7261 tttcgatgcg atggttaatc aaaaaatcaa tctggccgat accgataccc tgaaaaaatg
     7321 gctgtccgag caaacagcgt ttgacggcaa aaaagtattg gctgcatttg aggctcctga
     7381 aagccaagcg cgtgcggctc aaatggaaga gttgaccaat aaattccaaa tcagcggcac
     7441 accgactgtg attgtcggcg gcaaatacca agttgaattt aaagactggc agtctggtat
     7501 gaccacgatt gaccagttgg tggataaagt acgcgaagag cagaaaaagc cgcaataagt
     7561 tgaggattga atgagtaaag gccatctgaa aataggattt cagacggcct tttgtattta
     7621 ggctttatag aagagatgat tgcttaaagc cttatggttt taaatcagaa tatatag
//
LOCUS       DAWFAD010000095         7670 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWFAD010000095 DAWFAD010000000
VERSION     DAWFAD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7670
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.095"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(308..730)
                     /locus_tag="WGO49_001463"
     CDS             complement(308..730)
                     /locus_tag="WGO49_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ0306563.1"
                     /translation="MQVTSKWIDGMCFVGTTESGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(848..1519)
                     /locus_tag="WGO49_001464"
     CDS             complement(848..1519)
                     /locus_tag="WGO49_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ0306564.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1535..2161)
                     /locus_tag="WGO49_001465"
     CDS             complement(1535..2161)
                     /locus_tag="WGO49_001465"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ0306565.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AERGIPTDSLHHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGVVPILDGDTA
                     DDVAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2268..3074)
                     /locus_tag="WGO49_001466"
     CDS             complement(2268..3074)
                     /locus_tag="WGO49_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0306566.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAASEPAAASAAQGDTSSIG
                     STMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMM
                     KFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQP
                     SKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYI
                     PSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3333..3773)
                     /locus_tag="WGO49_001467"
     CDS             complement(3333..3773)
                     /locus_tag="WGO49_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ0306567.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3836..5242)
                     /locus_tag="WGO49_001468"
     CDS             complement(3836..5242)
                     /locus_tag="WGO49_001468"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ0306568.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLVDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5412..6527
                     /gene="lptF"
                     /locus_tag="WGO49_001469"
     CDS             5412..6527
                     /gene="lptF"
                     /locus_tag="WGO49_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ0306569.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGEFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6524..7594
                     /gene="lptG"
                     /locus_tag="WGO49_001470"
     CDS             6524..7594
                     /gene="lptG"
                     /locus_tag="WGO49_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ0306570.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRFFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
BASE COUNT         1663 a         2097 c         2021 g         1889 t
ORIGIN      
        1 aataaattaa tatatggctt aaaataacgg gatgcccgcc ttccgcccgc ccgcagaagc
       61 aggcgggtat cattttaaaa cgcggcattt aaaatttgac cgaaaattgt tgacaatccg
      121 gaatcaagtc tgcacaatac cccgacaagt ccaagtatta taaaggctga ataaagagga
      181 aacagcaggc agatatattc gggaggtgca gtccgaatat atctgctttt ttatgcgcct
      241 ccggattgcc tgccgcacct ttcccttcag acggcatcag ccgtttcccc ataatgccgc
      301 ccgatgccta tttatctgcc ccggcaattt caaaactgtg ggtaatcttt gccgctttgc
      361 ccaacataat cgaagccgaa cagtattttt cggcagacat ctgaacggcg cgctcaatgg
      421 ccgattcttt caaatcatgc ccgaatactt tgaaatggat gtggatttcg gtaaacacgc
      481 gcggcgcatc gtccgcccgt ttcgccgtaa ccgtcgcacg gcagtcagtc actttctgac
      541 gctgtttttc ggcaatcatc accacatcga tgctcgaaca gcccgccacg cccaacagca
      601 gcatttccaa agggctgggc ccgcgcttgg ccgcaccttc tgccgccgaa ccctccataa
      661 cgacgctgtg cccgctttcc gtcgtgccga caaagcacat tccgtctatc cattttgatg
      721 taacctgcat ggtgtcattc ctgaaaatag cgttaaaacc gctttgcata tggcgttatt
      781 gtaaacaatt tcaagcggct tatgcagaaa tatggacaaa acggcaaaaa aacgcctgaa
      841 aaccgattta cggtttggcg gactggccat tgatctgcac cgatttgagt ttcagcgtat
      901 aggttttgcc gtcgtcggta tagccgattt gtgccggaat attgttcagg gacggtgcga
      961 agaaatacat taccgtatcg tcgccgcgcc gcacccgata tttgacgact tcggtttcca
     1021 cgccgcctat gctgtatttt cctgtacccg ccttattcaa accgccgacg gaataaagtt
     1081 ttttgccgtt ggtgattttc agccccgggg ggagtttcgc gtcatttgcc gccaactgcc
     1141 aggcaagcgt gaacaaatcc atagccttgg ggctttgctc ggttttgctc tcgcccgctt
     1201 tgccgtaggt tacgctgccg tcggcgaatt tggcttccgc atacagtttg cccctgcgta
     1261 tgtctctata gtaggtaggg tgcagggtat tgccgacaac cgtaccgccg gactcgaaac
     1321 ggatattgta tagcggcact ttaatcgtcg aaacgatttt gtaagcattg ccgctgcgtt
     1381 caaatgtcat cgtggcggga atgccgtagc tgccggaata gtgcagcacg gcggattggg
     1441 gcagccctgc cgcatacgcg cacggcaggg cggcggacaa aatggcggcg gaaaatatat
     1501 ttttaaaagt cttcatcatt tgctcccgcc cggtttacgc cgtcagaaaa cgggcggcat
     1561 cggcgttttc cgaatttctg acgcggtttc cctcgataat caggcggccg gcggcaaaat
     1621 cggcaacggc tttcggatag agtttatgct cgacagccaa aacccgggcg gcaacatcgt
     1681 ctgccgtatc gccgtcgagt atcggcacaa ccccttgcga tacaatcggg ccgcaatcca
     1741 gttcggcagt aacgaaatgg atggtgcagc cggcaacgcg acaacctgcc tccaaagcgc
     1801 gttcgtgcgt atgaagtccg gtaaacgagg gaaggatgga cgggtgaatg ttcatcagcc
     1861 tgccttcgta atgggcacag aactcagggg tcagaatccg cataaaacct gccaaaacca
     1921 ccaagtcggg ttgatatgcg tcgatttttt ccatcatggc ggtatcgaag gcaagtcgtg
     1981 attcaaagtt tttatgatgc aggctgtcgg tcgggatgcc gcgttcggcc gcccattgca
     2041 aaccggcagc cgtttcgctg ttgctcaaca cggcggcaat gcggacgttg ggaatggcgg
     2101 cattgacgat tgcctgcata ttgctgccgc gtccggaaat caggatgacg atgtttttca
     2161 taatggtacg cttttgaaag ggatgccgtc tgaaccgctg tttggtggtt tcagacggca
     2221 tttgccgtaa aaatgcccga aaacctgttt cgggcatgga ttcggactta atttactttt
     2281 ttgatgtcga cttgagccgg ctgcttggcg ggcgcgtttt cgggtgcgcc gattttgacc
     2341 agtttcacat caaataccaa agtggcgttc gggccgattt tgtcgcccgc accctgttcg
     2401 cggtaggcaa ggttggacgg gatgtagaac gtggcttcgc cgccttcttt cagaagctgt
     2461 acgccttcgg tccaacccgg aatcacttgg ctcaaaggga aggtgaccgg gccgccgttg
     2521 gctttgctgc tgtcgaatac cgtaccgtca atcaggcggc cttcgtattc cacggtaacg
     2581 atgtcgtctt tggacggctg tttgccttca ccctgtttgg tgattttgta ttgcaggccg
     2641 gaagcagtgg tcttcacgcc gtctttggcg gcattttctt tcagaaaggc ttcgcctttt
     2701 tctttattgg ccttcgcgtc cgccttgtgt ttttctacgg ctttagcctg ttgttcctga
     2761 aggaatttca tcatgacttc ctgagcctgc tcttcagtca ttttgatttc tttgccgtca
     2821 tacactgcct gcatggcttc ggtaaagact ttcaaatcga tttccgcacc ctgttccttc
     2881 atttgcttca gggagcgtcc gatgtccacg cccatcgcat agcttgcctg ctgcatcgtg
     2941 ctgccgatcg aagaggtgtc gccctgcgcg gcagaagcgg cggcaggttc ggatgcggct
     3001 tcttttttgc cacaggcgga aagtgccaaa gcggcggaaa gggtcagtgc gctgattttg
     3061 aaaatggtgt tcatgatgga tcttcgctgt cgataaggtc ggaaaaacgg gattatagcc
     3121 gagtttgaat gtttcaacac acaggatgac acataaagcg tcaatcgtgt gttgccctgt
     3181 tttggaaggg attgaacctt ccaaaataag ttttgattct accgccccga gggacagatg
     3241 tccaagtggc ggggttcaac cgataaggaa attttaatca aatagaatca agcctgttta
     3301 aattttgtaa atgcggcatt tcagacggca ttttatgcct tgccctccat gccgtgatgt
     3361 tcgatggcaa aaccgcttcg gcggtaggcg gtaaagcgtt cgcgcgcgtc ggcgagatct
     3421 tccaagctgt tgccgacgat ttccaaaacg cgcgcgggca agaccggagc ggtgttccaa
     3481 aaaccgtcgg acaggttcaa aacagtcatg ccttcgggaa ttcggggcgg attgccgccg
     3541 caagcaagca ggacggatgt ttcagacggc atggcttctt ccatttccca aatttcgtgc
     3601 ggaatgaaac tctcggcttc gtattgccaa agcattttgt ccaattcctg aagccgcccg
     3661 aacgaatcgg accacaccag tatcctgccg ccgtcccgaa tcgcacgggc aatcaggcgg
     3721 caggtgaaaa tcggaacttg ggcaacgtgc gtgtaaaagg tggctttcgg catattgttt
     3781 gagcatttgg caggacgatg ccgtctgaaa ggcttcagac ggcattgtgg caaaattaaa
     3841 gattccgcag atagttcagc agcaagggaa cgggacggcc agtcgcacct ttttccgctc
     3901 cggatttcca cgccgtaccc gcgatgtcaa ggtgcgccca cgggtagcct tcggtgaagt
     3961 aagacaggaa tgttgcggcg gtaatcgtgc ccgcgccggg cgtgccgata ttggggatgt
     4021 cggcaaagtt ggatttgagc tggtctttgt aggtttcaaa gagcggcagt tgccacgctt
     4081 tgtcgtccac gttgtaggaa gcggcaagca ggctgtcgac caaatcttga ttgttgccca
     4141 tcacgccgct gacatcgtgc cccaaggcga caatacacgc gccggtcagg gtggcgacgt
     4201 cgatgacggc tttgggtttg aactgttcgg cgtaagtgag cgcgtcgcac aaaatcagac
     4261 ggccttcggc atcggtgttc aacacttcga tggtcagacc tttcatactt ttcacgacat
     4321 cgcccggttt gtttgccgcg ccggaaggca tattttcaca agtggcgacg acggcaatca
     4381 ggttaatcgg cagttgcagt ttgacggcgg cgcagaaggt gctgatgacg gttgccgcgc
     4441 cgcacatatc aaacttcatt tcgtccatgt tcaggccggg tttgagggaa atgccgccgg
     4501 tgtcgaaggt aatgcctttg ccgaccaata ccacaggcgc ggcttctttg tccgccgcac
     4561 cgaaatagct cagttcgacc aaatagggat cttcgacact gcctttggcg accgaccaaa
     4621 acgaacccat gttttctttg atgtagtctt tttcgatgat tttggcgtgc gcgcccagtt
     4681 tttcggcttc ggctttggcg gtgcgcgcta aaaattcggg cgtgcattcg ttgggcgcgg
     4741 cgttgcccaa gtcgcggcag aggctttgtc cgtaaacttg cgcttcggcg acgcgcaagg
     4801 cttctttaac gacggcttcg tgtgcgctgt ggaacacggc ggtttcaaat ttggcgggct
     4861 tggcttcttt tttgtagcgg tcgaaacggt aggcggcgtt gccgaacgca atcgcaaacg
     4921 cttcggctac ggcggcggct tgcgcttcat cgaaggcatg gatgtccaca ttgaccgttt
     4981 cctgattttg cgcccatttg gcggcttcgg cggcggcctt gttcaatgcg gcgcggccgg
     5041 tgcttttcag acagcatacg gcaacagcct gcaaaccgtt gcctgtcggg atttttgtgt
     5101 cggcaaaatt ttgaccttct tcaagcgaag acaaaagggc aagggcggtc ggatggttta
     5161 attgcgcctc ttcggtgcag acaaataact gtgcgcctgc ctgctgttcc tgcaagattt
     5221 cggtttttgt gctaaattcc acgtttattc tcctgattga gacggttgtc ggtagttttc
     5281 ggacggcctt tcgctcaaaa gaccgtctga agacggctgg cacgattgta ccccatttga
     5341 agcaccgtct gaaaccttgc gcggacaatc cgcctgcgcc gaaccgctta ccgccccctg
     5401 accgcgattc tatgatttat caaagaaacc tcatcaaaga actctctttt accgccgtcg
     5461 gcattttcgt cgtcctcttg gcggtattgg tctccacgca ggcaatcaac ctgctcggcc
     5521 gtgccgccga cgggcgtgtc gccatcgatg ccgtgttggc attggtcggc ttctgggtca
     5581 tcggtatgac gccgcttttg ctggtgttga ccgcatttat cagtacgttg accgtgttga
     5641 cccgctactg gcgcgacagc gaaatgtcgg tctggctatc ctgcggattg gcattgaaac
     5701 aatggatacg cccggtgatg cagtttgccg tgccgtttgc cgttttggtt gccgtcatgc
     5761 agctttgggt gataccgtgg gcagagctac gcagccgcga atacgctgaa atcctgaagc
     5821 agaagcagga attgtctttg gtggaggcag gcgagttcaa cagtttgggc aagcgcaacg
     5881 gcagggttta ttttgtcgaa accttcgata ccgaatccgg catcatgaaa aacctgttcc
     5941 tgcgcgaaca ggacaaaaac ggcggcgaca acatcatctt cgccaaagaa ggtaacttct
     6001 cgctgaacga caacaaacgc acgctcgaat tgcgccacgg ctaccgttac agcggcacgc
     6061 ccggacgcgc cgactacaat caggtttcct tccaaaaact caacctgatt atcagcacca
     6121 cgcccaaact catcgacccc gtttcccacc gccgtaccat tccgaccgcc caactgattg
     6181 gcagcagcaa cccgcaacat caggcggaat tgatgtggcg catctcgctg accgtcagcg
     6241 tcctcctact ctgcctgctt gccgtgccgc tttcctattt caacccgcgc agcggacata
     6301 cctacaatat cttgattgcc atcggtttgt ttttaattta ccaaaacggg ctgaccctgc
     6361 tttttgaagc cgtggaagac ggcaaaatcc atttttggct cggactgctg cctatgcaca
     6421 tcatcatgtt cgtcatcgca atcgtacttc tgcgcgtccg cagcatgccc agccagccct
     6481 tctggcaggc ggttggcaaa agtctgacat tgaaaggcgg aaaatgaacc tgatttcacg
     6541 ttacatcatc cgtcaaatgg cggttatggc ggtttacgcg ctccttgcct tcctcgcttt
     6601 gtacagcttt tttgaaatcc tgtacgaaac cggcaacctc ggcaaaggca gttacggcat
     6661 atgggaaatg ctgggctaca ccgccctcaa aatgcccgcc cgcgcctacg aactgatgcc
     6721 cctcgccgtc cttatcggcg gactggtctc tctcagccag cttgccgccg gcagcgaact
     6781 gaccgtcatc aaagccagcg gcatgagcac caaaaagctg ctgttgattc tgtcgcagtt
     6841 cggttttatt tttgctattg ccaccgtcgc gctcggcgaa tgggttgcgc ccacactgag
     6901 ccaaaaagcc gaaaacatca aagccgccgc cattaacggc aaaatcagca ccggcaatac
     6961 cggcatttgg ctcaaagaaa aaaacagcat tatcaatgtg cgcgaaatgt tgcccgacca
     7021 taccctgctg ggcattaaaa tctgggcccg caacgataaa aacgaactgg cagaggcagt
     7081 ggaagccgat tccgccgttt tgaacagcga cggcagttgg cagttgaaaa acatccgccg
     7141 cagcacgctt ggcgaagaca aagtcgaggt ctctattgcg gctgaagaaa actggccgat
     7201 ttccgtcaaa cgcaacctga tggacgtatt gctcgtcaaa cccgaccaaa tgtccgtcgg
     7261 cgaactgacc acctacatcc gccacctcca aaacaacagc caaaacaccc gaatctacgc
     7321 catcgcatgg tggcgcaaat tggtttaccc cgccgcagcc tgggtgatgg cgctcgtcgc
     7381 ctttgccttt accccgcaaa ccacccgcca cggcaatatg ggcttaaaac tcttcggcgg
     7441 catctgtctc ggattgctgt tccaccttgc cggacggttc ttcgggttta ccagccaact
     7501 ctacggcatc ccgcccttcc tcgccggcgc actacctacc atagccttcg ccttgctcgc
     7561 tgtttggctg atacgcaaac aggaaaaacg ttgaaccaat gccgtctgcc ccccttcgga
     7621 cggcattggt ttttattatc atattcccgc agacagatag ccgttcccta
//
LOCUS       DAWFAD010000096         7548 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWFAD010000096 DAWFAD010000000
VERSION     DAWFAD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.096"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            24..419
                     /locus_tag="WGO49_001471"
     CDS             24..419
                     /locus_tag="WGO49_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0306571.1"
                     /translation="MFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKAAAAGHGI
                     LYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHGNLGWLLF
                     AAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
     gene            complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WGO49_001472"
     CDS             complement(454..1329)
                     /gene="rsmI"
                     /locus_tag="WGO49_001472"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ0306572.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPVVMFETPHRIGATLADMAELFSERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            1376..1723
                     /locus_tag="WGO49_001473"
     CDS             1376..1723
                     /locus_tag="WGO49_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ0306573.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1728..2321
                     /locus_tag="WGO49_001474"
     CDS             1728..2321
                     /locus_tag="WGO49_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ0306574.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDILVGISTSGNSANVIEAIKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAICDCIDSVLLEGM"
     gene            2385..2993
                     /locus_tag="WGO49_001475"
     CDS             2385..2993
                     /locus_tag="WGO49_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ0306575.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            3001..3651
                     /locus_tag="WGO49_001476"
     CDS             3001..3651
                     /locus_tag="WGO49_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306576.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRAVLLICI
                     TAIGALAIGIVSTFNPNGDKTLQAEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            3691..4470
                     /gene="map"
                     /locus_tag="WGO49_001477"
     CDS             3691..4470
                     /gene="map"
                     /locus_tag="WGO49_001477"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ0306577.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGSVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            4625..4924
                     /locus_tag="WGO49_001478"
     CDS             4625..4924
                     /locus_tag="WGO49_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0306578.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            5162..5536
                     /locus_tag="WGO49_001479"
     CDS             5162..5536
                     /locus_tag="WGO49_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0306579.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(5724..7190)
                     /locus_tag="WGO49_001480"
     CDS             complement(5724..7190)
                     /locus_tag="WGO49_001480"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ0306580.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSTPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLCAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1945 a         2290 c         1846 g         1467 t
ORIGIN      
        1 aagaggcgga atgggcgggc agcctgttcg gcctgcacaa atctttcggc ttcctcacgc
       61 tgacggtgat tacattgcgc atcgtgtggg cagttgccaa ccgcgccaag cgtccgcaaa
      121 gcgacagcaa ggctgcggcg gcaggacacg gcattctgta tctgctcatg cttgccgttc
      181 ccgttatcgg catgatccgc caatacggca gcggccgcgg cccgttgaaa gtgttcggcg
      241 ttgaggtgat gcagggttcg ccggaaaaaa tcgagtggat ggcaaacttg ggcaacacgt
      301 tccacggcaa tttgggctgg ctgctgtttg ccgccgtcgt cggacacgtc gccatggtcg
      361 tcgtccaccg tgttcaaggc aaagacgtgc tgtaccgcat gacggggcgt gtccgttgat
      421 tccgttcgca ctatggtgcc ggtccgtccg gcatcatttg tttttccaag acagtgccag
      481 attgtacaaa gctttttttc cctcgcccgt gattttggcg gcaagctccg ccgcctgttt
      541 ggtcggcagc tcggctgtga ggattttcat gatgttttgc gcggactcgg acaagccttc
      601 gtgtttttca tcctgcgccg gataaagcac caacaccatc tcaccgcgcg attggttgcc
      661 gtcggcagac aatgccgtct gaatttcccc aaccgtgccg cttaagaacg tttcaaacgt
      721 tttcgtaatt tcgcgcgcca gcattaatcg gcgttcggag aacagttccg ccatatcggc
      781 aagcgtcgca ccgatgcggt gcggcgtttc aaacatcacg acgggaaacg ccgcccgcac
      841 ccatttggca aacagtttcc tgcgctctcc cgatttcggc ggtacaaaac cgttgaaata
      901 aaaatcggat cccgccacac cggccacact caaagccgcc atcaccgcgc ttgcgccgac
      961 aacagggaca actttaaacc cgacctcacg cacgcggcgg gcgagtttcg cgcccgggtc
     1021 gcacacggcc ggcgtacccg catcggaaac ctgtgccaca accatgccgt ctgaaagata
     1081 gccgacaatc ttgtccgcca tctgccgttc gttgtgttcg cgcacgctga caagtttgcc
     1141 ctgaatgccg tacgcgctca aaagctgcgc ggtaacgcgc gtgtcttcgg cacagatgat
     1201 gtccgccttt tgcaataccg ccaaagcgcg cagggtaatg tccgccaaat tgccgatggg
     1261 cgtggcaacc acgtataatg tccctccgac gacgctgtcg gaggctttct gcaagtgttt
     1321 ctgaaacata aaaaatgccg tctgaaaaac agacattata aaggttaaac cgattatgcg
     1381 cctaaaccac aaacagggcg aggcagggga agatgccgcg cttgccttcc tccaatccca
     1441 aggctgcaca ctgcttgccc gcaactggca ctgcgcctac ggcgaaatcg acctgattgt
     1501 caaaaacggc ggcatgattc tgtttgttga agtaaaatac cgcaaaaatc ggcaattcgg
     1561 cggtgccgca tacagcattt ccccatccaa attattgaaa ctgcaacgaa gtgtagagta
     1621 ttatctgcaa cagaacaggt tgacaaacgt accgtgccgc ctcgacgcgg tactcatcga
     1681 aggcagccgc ccgcccgagt ggatacagaa tattacaggt tgacgatatg acgacattac
     1741 aagaacgcgt tgccgcccat tttgccgaaa gcatccgtgc caagcaggaa gccggaaaag
     1801 tattggtcga gccgaccgta caggctgccg agctgatgct gcaatgcctg atgaacgacg
     1861 gcaagattct ggcgtgcggc aacggcggtt cggctgccga cgcgcagcac ttcgccgccg
     1921 aaatgaccgg acgctttgaa aaagagcgca tggaactcgc cgccgtcgcc ctgaccacag
     1981 acacctccgc gctgaccgcc atcggtaacg actacggctt caaccacgtc ttcagcaaac
     2041 aggtacgcgc cctcggacgc gccggcgaca tcctcgtcgg catttccact tccggcaatt
     2101 ccgccaacgt catcgaagcc attaaagcgg cacacgaacg cgatatgcac gtcatcgccc
     2161 tgaccggccg cgacggcggc aaaatcgccg ccatactcaa agacaccgac gttctgctca
     2221 acgtccccca tccgcgcacc gcccgcattc aggaaaacca catcctgctg atacacgcca
     2281 tatgcgactg tatcgactcc gtactgctgg aaggaatgta acccttttca gacggcatgg
     2341 cgcaaagcaa tgccgtctga aacgcccaag aaaggaagcc cccgatgaaa cccaaaccgc
     2401 acaccgtccg caccctgatt gccgccattt tcagccttgc ccttagcggc tgcgtcagcg
     2461 cagtaatcgg aagcgccgcc gtcggcgcga aatccgccgt cgaccgccga accaccggcg
     2521 cgcaaaccga cgacaacgtt atggcgttgc gtatcgaaac caccgcccgc tcctatctgc
     2581 gccaaaacaa ccaaaccaaa ggctacacgc cccaaatctc tgttgtcggc tacaaccgcc
     2641 acctgctgct gctcggacaa gtcgccaccg aaggcgagaa acagttcgtc ggtcagattg
     2701 cacgttccga acaggccgcc gaaggcgtat acaactacat taccgtcgcc tccctgccgc
     2761 gcactgccgg cgacatcgcc ggcgacactt ggaacacatc caaagtccgc gccacgctgt
     2821 tgggcatcag ccccgccaca caggcgcgcg tcaaaatcgt tacctacggc aacgtaacct
     2881 acgttatggg catcctcacc cccgaagaac aggcgcagat tacccaaaaa gtcagcacca
     2941 ccgtcggcgt acaaaaagtc atcaccctct accaaaacta cgtccaacgc tgactcggca
     3001 atgccgtctg aaccgccttc agacggcatt gcccgacacc ccaaaagcac aatcaaaatg
     3061 gcaaaaaaac cgaacaaacc cttcaggctg acccccaaac tcctgatacg cgccgtattg
     3121 ctcatctgta tcaccgccat cggcgcattg gcaataggca tcgtcagcac attcaacccg
     3181 aacggcgaca aaacccttca agccgaaccg caacacaccg acagcccccg cgaaaccgaa
     3241 ttctggctgc caaacggcgt agtcggacaa gatgccgccc aacccgaaca ccaccacgcc
     3301 gcctcatccg aacccgcaca gccggacggc acagacgaaa gcggcagcgg actgccgtcc
     3361 cctgccgcac ccaagaaaaa ccgggtcaaa ccgcaacctg ccgacacagc tcaaaccgac
     3421 aggcagccgg acgacgccgg aacacaagct gaaaacacac tcaaagaaac ccccgtactg
     3481 cccacaaacg tcccccgtcc cgaaccccga aaagaaacac ccgaaaaaca ggcgcagccc
     3541 aaagaaacac ccaaagaaaa agaaacgccc aaagaaaacc ataccaaacc ggacaccccg
     3601 aaaaacacgc cgcccaaacc ccataaagaa attctcgaca acctcttctg acccggcacg
     3661 gcaggcacac ccgcaatcca aggaagcatt atgaacggca tcatcatcaa aacccccgaa
     3721 gaaatcgaaa aaatgcgcga gctgggcaaa ctcgtcgccg aagccctcga ctacatcgga
     3781 caattcgtca aacccggcgt aaccaccgac gaaatcgaca aactcgttta cgactaccac
     3841 gtcaacgtcc aaggcggcta tcccgccccc ctgcactacg gcaacccgcc ctaccccaaa
     3901 tcctgctgca cctccgtcaa ccacgtcatc tgccacggca ttcccgacga caagccgctc
     3961 aaagaaggcg acattatcaa catcgacctc accatcaaaa aagacggctt ccacggcgac
     4021 tccagccgta tgtttaccgt cggcaaagtc tcccccatcg cccaacgcct gatcgacgta
     4081 acccacgcct ccatgatggc gggcatagaa gccgtcaaac ccggcgcgac actgggcgac
     4141 gtaggttacg cctgccaaca ggttgccgaa aacgccggct attccgtcgt acaggaattc
     4201 tgcggacacg gcatcgggcg cggtttccac gaagccccgc aagtgttgca ctacggaaaa
     4261 aaaggacagg gctccgttct aaaaccgggt atgattttta ccgtcgaacc gatgatcaac
     4321 caaggcaaac gccacctgcg tatcctcaac gacggctgga cggtggttac caaagaccgc
     4381 tccctctccg cccaatggga acacgaagtc ttggtgaccg aaaccggcta cgaaatcctc
     4441 accgtcagcc ccgcctccgg caaaccctga agccggacgt atccgcccca taaaaacaaa
     4501 caatgccgtc tgaaagaaac ggcagatatg atatataata taaaaacagg cttgacccgg
     4561 cacattacga aaacaaagca aatcggaatt tgccccgcaa ccagacaaac ttaaaggaag
     4621 ttttatgaaa atatttgaaa atatagaaga tgttaaagcc atccgtaaaa agaccgggct
     4681 gaaccagata gacttctggg gcaaggtcgg cgttacccag tctggaggct cgcgctacga
     4741 aaccggccgc aaaatgccca aacccgtacg cgaactgctc cgcctcgtcc atatcgaatg
     4801 catcgatttg gcgaaagtca acaaaaaaga tatggaaatc gccgccctgt tgaaaaaaca
     4861 ccatcccgac ctgtatgccg agttgtccaa acagaccaag tccgaaagaa aaaaacaaag
     4921 ttaaaccgca acctccggat gcccgacagt ttttcatttc cgaaaaacgc aaacaatgcc
     4981 gtctgaaaca ccggacaggt cgccgtatcc cgcctgccgc ccctgcctca aaccgccgaa
     5041 ccgcccgaac ccgccttttt acaaacttta tccaatttcc tgtttatttc aggatacgcc
     5101 gacattagaa tgtcaaacag ctcgaaacgg gcaaactcca catccatcca aaggaataaa
     5161 aatgaaactt ctgaccaccg caatcctgtc ttccgcaatc gcgctcagca gtatggctgc
     5221 tgctgccggc acgaacaacc ccaccgttgc caaaaaaacc gtcagctacg tctgccagca
     5281 aggtaaaaaa gtcaaagtaa cctacggctt caacaaacag ggtctgacca catacgcttc
     5341 cgccgtcatc aacggcaaac gtgtgcaaat gcctgtcaat ttggataaat ccgacaatgt
     5401 ggaaacattc tacggcaaag aaggcggtta tgttttgggt accggcgtga tggatggcaa
     5461 atcttaccgc aaacagccca ttatgattac cgcacctgac aaccaaatcg tcttcaaaga
     5521 ctgttcccca cgttaatcag gcaacaaaaa acagcgtttt cagaaatgaa aacgctgttt
     5581 ttttgaccgt tccattattc gcaaaaggga aaaaacgatt acctgccccg tgtatcaaaa
     5641 cctgccctgc cggatgaagg gcataaccgg cagggacggc atcaacacca tatgggggta
     5701 cggcttttct tgaaagattc ggcttaaata tccaacactt tcgcggtata ggcgataatt
     5761 tcatccgccc tttcggggtt ttcgttcaac ttgatgccgt aacccggtac cagctctttc
     5821 agacggcctt cccaagacgg ggcgcgctcg gggaagcatt ggtgcatcag ccggatcatc
     5881 agcggcacag cagtcgatgc gcccggcgac gcgcccagca atgcggcgag tgagccgtcg
     5941 gcgtgggcga caatctccgt accaaactgg agcacgccgc ctttttcgga gtctttttta
     6001 atgatttgga cgcgttgccc tgcggtgatg agttcccagt cgtcggggtt tgcctcgggg
     6061 tagtattcca gcagggaggc gaagcgttct tctttggttt tacgcaattc gcccagcagg
     6121 tatttggtca gcggcatatt cgcccagccg gcgcacagca taggatagag gttgtccata
     6181 tggatggaca gcggcaaatc cataagcgag ccttgcttga ggaagttgga acggaagcct
     6241 gcataagggc cgaacataag gtggcgtttg ccgtccacgt tgcgtgtgtc gaggtgcggg
     6301 acggacatcg gcggcgcgcc gacggaagcc tgcccgtaca ctttggcgtt gtgttgttcg
     6361 gcggtttcag gggtgctgtt gcggaagaac aggccggaca cggggaagcc gccgtagcct
     6421 ttgccttcgg ggatgccgga tttttgcagc agggtcagcg cgccgccgcc cgcgccgagg
     6481 aagaggaagc gggtacggag ggtgagctgc ccgtcggggt tgcgggtatc ggcggttttg
     6541 agcacccacg cgccgtcgga ttcgcgtttg atgtcttcga cgtggcggtt gaactcggtt
     6601 tttacgccct tgccctgcaa atatttcacc atttggcgcg tcagccgtcc gaaatcgaca
     6661 tccgtgcctt cggcggagta gttggcggcg acgggttggt tttcgtcccg gccgcgcatc
     6721 atcagcggag cccaatcgga aattttgttc cgatcggtgg aaaattccat attttcaaaa
     6781 agtttttggg ttttaaacgc gtcataacgt ttttgaagat aagaacaatg gtcttcattc
     6841 atcaccaaag acatatgcgg cacggcattg atgaaggaat tgtcttccaa cttgccttcc
     6901 gcgaccaacg tcgcccaaaa ctggcggctg acatgaaact gttcggcaat attgagggcg
     6961 cgcgccggat cgataatccc atttgcaccc aacggcgcat agttcaattc gcacagcgcg
     7021 gaatgccccg tgccggcgtt gttccacgcg tttgacgatt ccaacgccac atcttccaag
     7081 cgttcaatca gggtgatttc ccaagacggt tcgagttctt tgagcaaaac gcccaaagtc
     7141 gcgctcataa tgccgccgcc caccaagaca acgtctgtcg cttcagccat ggtttactcc
     7201 taaaaaacag gcatcttctg cccttatggt tatttgccgt actacaaacg cctgaatcgc
     7261 aaaagcaggg aaaaccggca atggtgtgtg tccgagtatg ctgtttcggg gttggaatgc
     7321 gttgcaagta tggcttccga caccgcttca ggggcttgta atatgttatc gtgaatgtag
     7381 tggattttac tgggaaatgc aaagtttttc tgtcgcccgc caagcccggg aactgcgaaa
     7441 tgaaaaataa aaatagttat ttatctatat atatcaaatt tttaatagat aaaaaaccaa
     7501 aattgtttat atattaattt ttaaaagatt gtcagcatat tgcgttaa
//
LOCUS       DAWFAD010000097         7479 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWFAD010000097 DAWFAD010000000
VERSION     DAWFAD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.097"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(16..771)
                     /locus_tag="WGO49_001481"
     CDS             complement(16..771)
                     /locus_tag="WGO49_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ0306581.1"
                     /translation="MKYVRLFFLGAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(896..2055)
                     /gene="nirK"
                     /locus_tag="WGO49_001482"
                     /pseudo
     CDS             complement(896..2055)
                     /gene="nirK"
                     /locus_tag="WGO49_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
     gene            2429..2713
                     /locus_tag="WGO49_001483"
     CDS             2429..2713
                     /locus_tag="WGO49_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NorB protein"
                     /protein_id="HEZ0306582.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELVA"
     gene            2714..4684
                     /locus_tag="WGO49_001484"
     CDS             2714..4684
                     /locus_tag="WGO49_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ0306583.1"
                     /translation="MDLTAQQTYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSV
                     VISDTRVKAIESILPYYHGVYGDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTS
                     WSASTNRPDETFTYTNNWPHEPLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLT
                     KHEEVEVPSEDPISKIQLTPSQKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYG
                     IDEALGFEMSDWFPYALTRTWHIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGV
                     NFLYIALFIVVGGSYAGNFFALTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLL
                     WLFLMLRCTVSAFKEKGVDKNLLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWW
                     VVHLWVEGFFEVFATAAFAFVFYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYF
                     SGSTSASMAIGACFSALEVVPLVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFW
                     NMIGAGVFGFLINPPISLFYIQGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNA
                     QFDDKLMTWGFWLLNGGLVGMIAISLLPVGVIQAYASITHGLWYARSEEFLQMEILDT
                     LRWVRTAADLVFIGGAICVAIQATKIVFGRDK"
     gene            4863..5396
                     /locus_tag="WGO49_001485"
     CDS             4863..5396
                     /locus_tag="WGO49_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ0306584.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5459..6643)
                     /locus_tag="WGO49_001486"
     CDS             complement(5459..6643)
                     /locus_tag="WGO49_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ0306585.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNAGFTVTNQQAGSRRF
                     QRFAGTDAQRVADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGILWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6683..7087)
                     /locus_tag="WGO49_001487"
     CDS             complement(6683..7087)
                     /locus_tag="WGO49_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ0306586.1"
                     /translation="MTAQPCPICTAQDEDILLQTPKLRVIAVHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1654 a         1826 c         2090 g         1909 t
ORIGIN      
        1 ctataattga aggggttatc ggcttgtgca acggaagccc aagttgtgca agacatattt
       61 ggattgcagg ctggtgcgga tgccgtagcg gaggaaggcg gcatagttgg acgagtcgct
      121 cgacccgata gacgcgccgc tgcaaaacat ttgcgcgttg gcattgccgg aagaaagcag
      181 gctgctgttg aaatcttccg tccattccca aatcagcccg tgcatatcat aaacgcccca
      241 gtagttcggg cggcctttgc cgacatcgtg caggcctttc cgtccgccgt cggcatacca
      301 atcgagaata gtgcggttgt agccgggttc gtttgagccg tttttctgcg tggcggaagc
      361 aagtccggca aattcccatt cgtcaatggt cggcaggcgt ttgccttgtg cggcgcaata
      421 ggcgttggcg gcaaaccagg aaacatttgt taccggttgt tttaattcgc ccgccttcgg
      481 cgcatagctg cggctgccgt ttttcatcca atgcttcagg taagcgggtt ctgcctgttt
      541 ggaaccgatc ctgccttttt gccattgggg gtggctgttg acaaattcgg caaactcggc
      601 attggtaacg ggatatttat ccagtttgaa cggtttgact ttaatcaggc cggtatcttt
      661 tttcagataa agcgggcggt agctgccgcc ttcgatttga accatttcgg cagccgccgc
      721 ttgagtgccg gcgagtgccg cgccgaggaa aaataaccgg acatacttca taaagcctcc
      781 tgacaggcgg ttaaaatcaa tcttccgaaa ggaaagattg gttgttaaaa aaccactgcc
      841 gtgcgtaatg aagtacagcg gcagtggttc gtcccgctaa tgacgggtat ccaatttaat
      901 aaacgctttt ttcggatgca gaggctgccg gggcagaagc tgcgggagcg gaagcagcag
      961 gagctgcacc gttaccggcg taagcggtat cactcaattt ttgagtcatg atttcagggt
     1021 tttccgcacc ctctactttc aattggccca acgcgccttt gttgaatgcg cggaagatgg
     1081 agtggtcgac caaagtgtag ctgcccggga tgtcgacttt gaattcgacg atggcggaac
     1141 cgccggcagg aacgatggtg ctttgtacgt tttcgttaat cagcttgccg ccttccacat
     1201 aaactttgtc gaagatttcg ccgatgacgt ggaaggaaga cactaggttc ggaccgccgt
     1261 taccgacgta catacgcacg gtttcgcctg ctttggcttt cagcgcgtta tcgccggcga
     1321 tagaacctac atgaccgttg aatacgacgt attcaggctg ttcggcaacg gctttgtcca
     1381 tatcgaacgg ttgcaggcct tgcgcgcctt ttttgccttt ggtgtagaag tcgccttgga
     1441 cgatgtagaa ctctttatcc actttcggca gaccttcttt aggctcgacc aaaatcagac
     1501 cgtacatacc gttggcgatg tgcataccga ccggcgcgac ggcgcagtgg tagatgtaca
     1561 gacccggttg cagggctttg aagctgaatg tggaagtgcg gcccggggcg gtaaaggttg
     1621 cggctgcacc gccgccctga ccggttgcgg cgtggaagtc gacgttatgg ggaacggtag
     1681 aagacgggtt gttggaaaat tccacttcaa ccgtatcgcc ttcgcgtacg cggatcatgc
     1741 gacccggaac gtcgccgtca aatgtccagt agcggtattc cacaccgtct tccatggtca
     1801 tggtttttcg acagtttcca tttttacgcg gactttggca ggatagtcgc ggtcgattgc
     1861 gggaggtact tcaggagcgt gggtggtaac tgcatcgata acgggcagtt cgcctgtcgg
     1921 cgtttcggcg gcggtttgtg cggcggaact tgcggcttct gcggaagcgg caggggcttg
     1981 cgcggcaggt tcgccgccgc aggcggctaa tgcgaataag gaagcaatca ttgcagctaa
     2041 ggcttggcgt ttcataatgt tttccttttg taagaaaagt agaggaattg gttaagaact
     2101 tggtgtgatt atgcgtttgc cccattgggg gcattttgat ttaagtcaaa taattcataa
     2161 aatatgaact taaaatgata cttctaatac tttatagcta ctaaaaatta caatgaatag
     2221 gctgatttgc ttaagggctt cggcaaatca ggcaaaggca gggtagttgc cgactaaaga
     2281 aaatgatagt tattatcata tatgtatttg ttttatatgt gagtttcttt gaacaaatgt
     2341 ttatttgtgc gctaaaccgt gctacaatct ttaacattca tattctgtga attttaaaca
     2401 accactttta ccaaaaggag ctctaaaaat gggacagtac aagaagctat ggtacttgct
     2461 gtttgccgtt ctggcggtat gctttaccat tcttggctat atgggcagcg aggtttacaa
     2521 aaaagccccg ccttaccctg aacaggtcgt ttccgcatca ggcaaagtgc tgatgacgaa
     2581 agacgatatt ttggcaggtc agtctgcgtg gcagagtacc ggcggtatgg aagtcggttc
     2641 gattctgggt cacggcgcat atcaggctcc ggactggacg gccgactggc tgcatcgtga
     2701 gttggttgca tagttggatt tgaccgcgca acagacttac ggcaaaaaat tcgatgaagt
     2761 ttcccctgaa gaacaagccg ttctgaaaac ccgactggct gacgaatacc gcaaccaaag
     2821 ccgtattaaa gaagacggca gcgtcgtcat cagcgatacg cgcgtgaaag ccatcgaaag
     2881 catcctgcct tattaccacg gtgtttacgg cgatgatccc gcgctgcaaa ccacccgcga
     2941 acactttgca atgaaaaaca acacattgcc aagccaagaa gcgcgtgaaa aactgttcga
     3001 cttcttcttc tggacttctt ggtctgcttc gaccaaccgt cctgacgaga cgttcactta
     3061 taccaacaac tggccgcacg agcctttgat caacaacgta ccgaccactg aaaactatat
     3121 gtggtcattc accagcgttg ttcttctgct gatgggcatc ggtttgttga tgtggggtta
     3181 ttccttcctg accaaacacg aggaagtgga agtgccgtct gaagacccga tttccaaaat
     3241 ccaactgacc ccttcacaaa aagcattggg caaatacgtc ttcctgacgg ttgccctgtt
     3301 tgtggtacaa gtattgctgg gcggtctgac cgcgcactac accgtcgaag gtcagggttt
     3361 ctacggcatt gacgaggcgt tgggctttga aatgtccgac tggttccctt acgccctgac
     3421 ccgtacttgg cacatccaat ccgccatctt ctggattgca accggctttt tgacggcagg
     3481 tttgttcctt gcgccgattg tcaacggcgg caaagatccc aagttccaac gtgcaggcgt
     3541 gaacttcctc tatatcgccc tcttcatcgt ggtcggcggc tcttacgcgg gcaacttctt
     3601 cgcgttgacg cacatccttc ccccggaatt taacttctgg ttcggacacc aaggttacga
     3661 atacctcgat ttgggacgtt tctggcaact cctgctgatg gtcggcctgc tgttgtggct
     3721 gttcctgatg ttgcgttgca cggtttccgc cttcaaagaa aaaggcgtgg acaaaaacct
     3781 gctggcaatc tttgtcgctt ctatggtcgg tgtgggcgta ttctacgcac cgggactgtt
     3841 ctatggcgag aaatccccga ttgccgtaat ggaatactgg cgttggtggg tggttcacct
     3901 gtgggtggaa ggcttctttg aagtatttgc cactgccgct tttgcctttg tcttctacaa
     3961 tatgggcttt gtccgccgca gtaccgccac agcttctact ctggccgctg ccgccatctt
     4021 tatgttgggc ggcgttccgg gtacgctgca ccacctgtac ttctccggct ctacctccgc
     4081 ctctatggca atcggtgcct gcttctccgc tttggaagtc gtgccgctgg tgttgctggg
     4141 ccgtgaagca tacgagcatt ggtcttacca acacctgtcc gactgggcga aacgcctgcg
     4201 ttggccgctg atgtgcttcg tggcagtcgc cttttggaat atgatcggtg ccggtgtatt
     4261 cggtttcctg attaacccgc cgatttccct gttctatatc caaggtttga atacttctgc
     4321 tgttcatgcg cacgccgcct tgttcggtgt gtacggtttc ttggcattgg gcttcgtatt
     4381 gctggttgcc cgttatttga aaccgaacgc gcagtttgac gacaaactga tgacttgggg
     4441 cttctggctg ctcaacggcg gcttggtcgg tatgattgcc atcagcctgt tgccggttgg
     4501 cgtgattcag gcatacgcct ccatcacgca cggcctgtgg tatgcacgca gcgaagagtt
     4561 cctgcaaatg gaaatcctcg acaccttgcg ttgggtacgt acggcagctg acttggtctt
     4621 tatcggcggt gcaatctgcg ttgccatcca agccactaaa atcgtattcg gccgcgacaa
     4681 ataagttttg cggtatgaaa tgagcaactg cacccgtttt cgggtgcagt tttttattgc
     4741 ggattgggac gggaatggaa aaggatgtgg attttttatt ttaatcggct atacaattgc
     4801 tgctttcatg ccgtctgaag cacgtttcag atggcatcgg tttacatcag ataaggataa
     4861 aaatgggtat ttacgatttt caaatgaaag atgcggaagg caatgcggtt gatttgtcag
     4921 gctatcgcgg caaggttctg ctgattgtca acacggcaac gcgttgcggt ctgaccccgc
     4981 aatacgaggc tttgcagaag ctgtatgcac aatataccgc agaaggcttg gagattttgg
     5041 attttccgtg caaccagttc cgcgaacagg ctcccgaaag cagcggagaa attgcccaag
     5101 tgtgtatgat gaagtttggc acgaagttta agattttcga caaaatcgaa gtcaatggag
     5161 caaataccgc gcccttgtat gcctacctga aatccgtcaa accgcaagat aaaggcaatc
     5221 atctgtttaa agatttcgtg ttgaagctcg cagctttggg agagaagcgc gacgaaggcg
     5281 acatcaaatg gaactttacc aaattcctcg tcaaccgcga cggcgaagtg gtcgagcgtt
     5341 ttgcgcctag tgttacgccc gaagaaatcg aagccgatat tcgggcattg ctgtaatctg
     5401 ccgccgtctt gggaaatgcc gtctgaaggc ttcagacggc atttgtccgt ttgcgggttt
     5461 attccgaaat atcggcaaca ccgctttcgg ggaagatggg caaatccggc ggtgggagca
     5521 gggtatccaa agtcagggcg gacgcatcga gtgtggggta gccttcaaac gcgaccgaat
     5581 aaccgctgtt tccgttcata cggattcggg cgtgcgcgcc cagagggaaa gtgattttgt
     5641 cggcaatgtg cccgaaggga aagcccgtca gcacggggat tttcgccgtg cgcgaaacat
     5701 ggttggcaac ggcggaaaaa tcataagacg gatcatagac atctcgaatt ttttccatac
     5761 ggaaattgcc gaacacgatg gcgcgctgtt tcttcaaaat acccgaaaga tacagcgtat
     5821 tgagcatacg ttcgatgcgg tagggctgtt cgccgacatc ttcgaggaac aaaatgccgc
     5881 cgtcgatgtc gggcatataa ggcgtgccgg cgagcgaggc gaggacgctt aagttgccgc
     5941 cccacaatat gccttcggtt tcgacatcgg cgcgttggat ataaggaaca tcaacggtca
     6001 ggcggttttg ggttgcaccc ttgataaagg catccatcgt aaacgcaccg ggggcgggtt
     6061 tgccaaaatc gctataagcc atcgggccgg caaaactcat catattgcct tttgccaaca
     6121 atgccagctg gacggcgcat acgtcgctga atccgaaaaa cagcgtgccg tgttcgcgca
     6181 tccttgcgcc gagcgaagca aaatcgatat gcggcagaat ccgcgccgca ccgtaaccgc
     6241 cgcgcaagcc catcagcact ttaggcgtgg cgacgcggcc ggaagcgacc tcttggaaat
     6301 cggcgacacg ttgtgcgtcc gtaccagcaa accgttggaa gcggcggctg cccgcctgtt
     6361 ggttggttac ggtaaaaccg gcattgtaaa ggcgggttaa ggctgtgttg acgcggttgg
     6421 tgtcttcggc aaagcctgac gaagcgacaa cgcgcagaag gttgccgtcc gaactttgac
     6481 ggcgtggcgt ttggagaggc acggttcggg ctttcacaac agaatgggaa gagggaaggg
     6541 gcggaacgga tgtggcggat gtgccgcaag cctgaagcag tcccgcgccg gcggcggcgg
     6601 tgcaggtttt cagaaaacga cggcgggagg tcggttcggt cataatttcc ctttcatacg
     6661 gtttcagacg gcatcggcgg gcttaaagca gctttttaag ctgttccgtc caatcttgcg
     6721 gcagggtcat accgtgtttt cggacggggt ttgcccaaat cggcgcaggg aaggacgcat
     6781 cgttttcaaa gcgggcgata atatgccaat gcaggtgcgg cacgacattg cccaagctgg
     6841 cgaggttaat cttggcaggt tgaaacactt ggcgcatagc ggcttcgact ttgtacacca
     6901 tttccatcaa ttcgccgcgt tccgctgccg aaaggtcggt catttcggca atatgcttac
     6961 gccaaatgac gcggcagaat gcaggcgaac cgctgtcgtt atggacggcg atgacgcgca
     7021 gcttgggggt ttgcagcaaa atatcttcat cttgtgccgt gcagatgggg cagggttggg
     7081 cggtcatttt tccttattcc tgaaagtgtt taaaaaacgg tagggtaatt tttaatattt
     7141 caatctgtct gtccgcattg ccgttaattg taccacttca tagggcggtg ctctatgtgt
     7201 cgtcctgtgt gttgaaacat atcggacgcg tttgaacagt tgtgcggcaa actgttttag
     7261 cggcaggcaa gagatgccgt ctgaagccca ttgtccgtca ttcccacaca ggcagttgct
     7321 acggttgctg aagcccctta gccgtcgcgc aggcggttgc tacggttact aaaatcccgt
     7381 cattcccgcg caggcgggaa tccagtccgt tcggtttcag ttgtttctga taaatcttgc
     7441 agcgttgggt ttttagattc ccgctttcgc gggaatgac
//
LOCUS       DAWFAD010000098         7362 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWFAD010000098 DAWFAD010000000
VERSION     DAWFAD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.098"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            46..990
                     /gene="tehA"
                     /locus_tag="WGO49_001488"
     CDS             46..990
                     /gene="tehA"
                     /locus_tag="WGO49_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HEZ0306587.1"
                     /translation="MSASKKFPIPLSYFSIALGLFALGLSWRYGASVGLLPALAAESL
                     LAAASVVWLLLVAAYLIKMFAYRNDFLSDLRDLVQCCFISAIPITAMLEGLALKPYQA
                     GAAAVLIYVGVAGQLAFSMYRAAGLWRGLHSLEATTPIIYLPTVATNFVSASSLAALG
                     HHDYAALFFGAGMFSWLSLEASILGRLRTAAPVGTAARGVVGIQLAPAFVGCGAYFAV
                     GGKVDGFALALIGYGCLQLLFLLRLTRWFWEGGFTMSFWGFSFGFAAMAGCGLHLAAS
                     GVLSGLGLTLATAGSAGVALLLVGTLHRIATGRFLVRG"
     gene            complement(1064..1747)
                     /locus_tag="WGO49_001489"
     CDS             complement(1064..1747)
                     /locus_tag="WGO49_001489"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ0306588.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDKDIMGLEIPTGQPLVYKLDDN
                     LKVLEKFYL"
     gene            2044..4347
                     /gene="parC"
                     /locus_tag="WGO49_001490"
     CDS             2044..4347
                     /gene="parC"
                     /locus_tag="WGO49_001490"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ0306589.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSSK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRVVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAVFRLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEIQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTTFKEGDRLKQTLEGRTV
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTVDSGETVLPPVAVYASSLINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNTGESMTHTAVSSEAEILIESEGRRGAAHKDRIPV
                     SLLEAKRGKKGRLLPISGSLKQLSSPK"
     gene            4368..5885
                     /locus_tag="WGO49_001491"
     CDS             4368..5885
                     /locus_tag="WGO49_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0306590.1"
                     /translation="MVISNPRELEKLKDRIPNLINIARIAIVLPLLIMHIFGLEVSGH
                     ENLYASWTTWAFYLWLAISCWMIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVGSSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
                     TASATFILVAASYLSAIFTSLSVKYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAV
                     VVINVEHQTILFNKKAKDLLPMLEIGQHTVLFDPITVLWDKASSRTFERNIDTPALTA
                     RIRAVPMNKEQNKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFW
                     AEFKQEFLLGHPDAAGCIRPDIQGGSLTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWHTPTSAI"
     gene            5937..6404
                     /locus_tag="WGO49_001492"
     CDS             5937..6404
                     /locus_tag="WGO49_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEZ0306591.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNVDQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            6508..7260
                     /locus_tag="WGO49_001493"
     CDS             6508..7260
                     /locus_tag="WGO49_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0306592.1"
                     /translation="MTDILIDNTATETVRTLIQAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         1672 a         2214 c         1912 g         1564 t
ORIGIN      
        1 cactatatct gtgcgttacg acggcatgcc gtctgaaggg tgtttatgtc tgcatctaag
       61 aaatttccga ttcctttgag ctatttcagc atcgcgctgg gcttgtttgc cttggggctg
      121 tcgtggcgtt acggcgcgtc tgtcgggctg ctccccgcct tggccgccga atcgctgctt
      181 gcggcggctt cggttgtctg gctcttgctg gtggcggcat acctgatcaa aatgtttgcg
      241 taccgaaacg attttttgtc tgatttgcgc gacttggtgc aatgctgctt catcagcgcg
      301 attccgatta ccgctatgct ggagggactc gcgctgaagc cctatcaggc aggcgcggcg
      361 gcagtcctga tttatgtcgg cgttgccgga cagttggcgt tttcgatgta tcgggcggcc
      421 ggtctgtggc gcggcctgca ttccttggag gcgacgacgc cgattattta tctgcctacg
      481 gttgcgacaa actttgtcag cgcgtcatct ctggcggcgt tggggcatca tgattatgca
      541 gctttgtttt tcggcgcggg tatgttttcc tggctgagct tggaagcctc catcttgggc
      601 aggctgcgca cggcggcacc ggtcggcacg gcggcgcgtg gcgtggtcgg catccagctt
      661 gcgcccgcct ttgtcggctg cggcgcgtat tttgccgtcg gcggtaaagt cgacggtttt
      721 gcgttggcat taatcggcta cggctgcctg cagcttttgt tcttgctacg cctgacccgc
      781 tggttttggg aaggtggttt tacgatgagc ttttggggat tttcattcgg tttcgcggca
      841 atggcaggat gcggtctgca tctggcggct tctggcgtat tgtcgggctt ggggctgacg
      901 cttgccaccg ccggatcggc aggcgtggcg ctgctgcttg tcggtacgct gcaccggata
      961 gcgacggggc gtttcttggt acgcggctga tgcgttttgc cgccttgtca aaaatgccgt
     1021 ctgaacgctc aagccttcag acggcatttt ttatttcaca cccttacagg tagaattttt
     1081 cgaggacttt caaattgtcg tccaatttgt acaccaacgg ctgaccggtc gggatttcca
     1141 agcccatgat gtctttgtcg gaaatgccct cgatgtgttt tgccagcgcg cgcagggagt
     1201 tgccgtgcgc cgccaccaag acgcgtttgc cgctcaaaat cgcgggggcg atttggtctt
     1261 cccaaaacgg caatacgcgc tccagcgtta ctttcaggtt ttcgccgtcg ggtacgacat
     1321 cggcaggcag atgggcatag cggcggtctt tgtgtgcgga aaactcatcg tctttgtcca
     1381 aaagcggcgg cagggtgtcg tagctgcgcc gccagatgcg gacttgctcg tcgccgtatt
     1441 gttcggcggt ttgttttttg tccaggcctt gcagttggcc gtagtggcgt tcgttcagcc
     1501 gccatgtttt gatttgcggt acgaacagtt ggtcggattc ttccaaaacg atgttgcagg
     1561 tcttaatcgc gcgggtcagg acggatgtga aggcgatgtc gaactcatag ccgttttctt
     1621 tcagtttttt accggcggcg gcagcctcgg caagcccctg ctcgctcagc ttcacgtcgc
     1681 gccagcctgt aaacaggttt ttcgcgttcc attcgctttg tccgtggcgg ataaatacca
     1741 gttccatatc gtctccaatg tgtgaaagtg ggaaagcctt atttataaca tattttcaca
     1801 tttcccgtat ttgattcaga ttcagacacg cgcccactat ggtttgccgt tttgatttac
     1861 aataatgtcc tttgttttac attccgcata cacaatgttt caacacacag gacgacacat
     1921 aaagcaccgc cctatgtgtt gccctgattt ggaaggggtt gccccctccc aaataaagtc
     1981 tgattctacc gccccgaagg acagatgtcc aagtggcggg gtttcaaccg aaaaggaaat
     2041 acgataaata tgcaaccgca cgctccacat accgattcca atacgctgat gctcggccga
     2101 tacgccgaac gcgcctatct cgaatacgcc atgagcgtgg tcaaaggccg cgcgctgcct
     2161 gaagtttcag acggccaaaa gcccgttcag cggcgcattt tgtttgcgat gcgcgatatg
     2221 ggtttgacgg cgggggcgaa gccggtgaaa tcggcacgcg tggtcggtga gattttgggt
     2281 aaataccatc cgcacggcga cagttccgcc tatgaggcga tggtgcggat ggcgcaggat
     2341 tttaccttgc gctatccctt aatcgacggc atcggcaact tcggttcgcg cgacggcgac
     2401 ggtgcggcgg cgatgcgtta caccgaagcg cggctcacgc cgattgcgga attgctgttg
     2461 tccgaaatca atcagggaac ggtggatttc gtgccgaact acgacggcgc gtttgacgag
     2521 ccgctgcacc tgcccgcccg cctgcctatg gtcttgctca acggcgcgtc aggcatcgcg
     2581 gtgggcatgg cgaccgagat tccgtcgcac aatttgaacg aagtgacgca ggcggcgatt
     2641 gcgctgttga aaaagccgac gctggaaacc gccgacctga tgcaatatat tcctgcgccc
     2701 gattttgccg gcggcggtca aatcatcacg ccggcggacg aattgcgccg gatttatgaa
     2761 accggcaagg gcagcgtgcg cgtgcgtgcg cgttatgaaa tcgaaaaatt ggcgcgcgga
     2821 caatggcgcg tcatcgtaac cgagctaccg ccgaacgcca attcctccaa aatccttgcc
     2881 gaaatcgaag agcagaccaa cccgaaaccg aaagcaggca agaagcagct caaccaagac
     2941 cagctcaata ccaaaaagct gatgctggat ttaatcgacc gcgtgcgcga cgagtccgac
     3001 ggcgaacatc ccgtacgact ggtgttcgaa ccgaaatcca gccgcatcga taccgatacc
     3061 ttcatcaaca cgctgatggc gcaaacttcg ctggaaggca atgtgtccat gaacttggtg
     3121 atgatgggtt tggacaaccg ccccgcgcag aaaaacctga aaacgatttt gcaggaatgg
     3181 ctggatttcc gcgtcgttac cgtaacacgc cgtctgaaat tccgtttgaa ccaagtggaa
     3241 aaacggttgc acatcctcga aggccgtctg aaagtctttc tgcacatcga cgaagtgatt
     3301 aaagtcatcc gcgaatcaga cgacccgaaa accgatttga tggcggtgtt caggctgacc
     3361 gaaatccaag ccgaagacat tttggaaatc cgcctgcgcc agttggcgcg tttggaaggt
     3421 ttcaaactcg aaaaagaatt gaacgaattg cgcgaggaac aaggccgtct gaacatcctt
     3481 ttgggcgacg aaaacgaaaa acgcaagctg attatcaaag aaatacaggc ggatatgaaa
     3541 caatacggcg acgcgcgacg cacgctggtg gaagaagccg gacgcgccgt gttgacgcag
     3601 accactgccg acgaacccat cacgctgatt ctgtcggaaa aaggctggat acgcagccgc
     3661 gccggacaca accttgattt gagccaaacc acgttcaaag aaggtgaccg cctcaaacaa
     3721 acccttgaag gccgcaccgt tttacctgtc gtcatcttgg attcatcggg cagaacctac
     3781 acgctcgatg ccgccgaaat ccccggcggg cgcggcgacg gcgtaccggt ttcatcctta
     3841 atcgagctgc aaaacggcgc gaaacccgtt gcgatgttga caggattgcc ggaacaacat
     3901 tatttattat caagcagcgg cggctacggc ttcatcgcca aactgggcga tatggtcggg
     3961 cgcgtgaaag cgggcaaagt agtgatgact gtggatagcg gcgaaaccgt cctgccgccg
     4021 gttgccgtct atgcttcttc gctgatcaac cccgactgca aaatcattgc cgccaccaat
     4081 caaaaccgcg ccctcgcctt ccccatcggc gaattgaaaa ttatggcgaa aggcaaagga
     4141 ctgcaaatca tcggattaaa caccggtgaa tcgatgacgc ataccgccgt ttcttccgag
     4201 gcagaaatcc tgattgaaag cgaaggcagg cgcggcgcag cgcacaaaga ccgcatcccc
     4261 gtctccctgc ttgaggcaaa acgcggcaaa aaaggcagac tattacccat atcgggcagc
     4321 ctgaaacagc tttcttcccc caaataaacc cgattccaca cataattatg gtgatttcca
     4381 acccccgcga acttgaaaaa ctcaaagacc ggattcccaa tctgatcaac atcgcccgca
     4441 tcgccatcgt cctccccctg ctgattatgc atatcttcgg gttggaggtc agcgggcatg
     4501 aaaacctgta cgcctcatgg acgacatggg cattttactt gtggctcgcc atttcctgct
     4561 ggatgatttt cttttccatc atccatccgc attggcaatg gcagtcgctg aaaatgccgc
     4621 gtttcagcgc ggtggcggac atcacgatga tcggcgtgct gacctacctg ttcggcggca
     4681 tcgactcggg gttcggcatc ctgatcctgc ccttcgtggg cagctcctgc ctgctcagtt
     4741 acgggcgtta ccccctgctc tatgccagct acgccgccat cctgctgata ttcaacgcca
     4801 ttgccgacgg cgatatcggc aaatatccgc acatatcgga tgcccgaacc gcctcggcaa
     4861 ccttcatcct cgtcgccgcc tcctatcttt ccgccatctt cacctcactg tcggtcaaat
     4921 acatcgacag cgccggccaa ctggcccgcg acaaccacct cgcctaccgg cgcatcaaag
     4981 gcttgagcca aaccgtactc gaacgcgttc aagaagccgt cgtcgtcatc aacgtcgagc
     5041 atcagaccat actgttcaat aaaaaggcaa aagacctgct acccatgctt gaaatcggac
     5101 agcatactgt cctgttcgac cctattaccg ttttatggga caaggcctct tcgcgtactt
     5161 tcgaacgcaa tatcgacacg cccgccctga ccgcccgcat ccgcgccgtg ccgatgaaca
     5221 aagagcagaa caagctgctc atcctctaca tccgcccgca aagcgaaatt caggcagaag
     5281 ccctgtccgt caaacttgcc gcactcggac aactgaccgc caacctcgcc cacgaaatcc
     5341 gcaacccgat gtccgccatc cgccatgcaa acgacctgtt gcgcgaaaat atggaagcgg
     5401 gggcggcaga tccgttcaac gccaaattgt gcaaaatcat cgacggcaac gtctgccgta
     5461 tcgacaaaat gctcgaagac atttcctcgc tcaacaagcg caacaaaacc gaacgcgaaa
     5521 ccatcggcct gataccgttt tgggcggaat tcaaacaaga gttcctgctc ggccatcccg
     5581 atgccgccgg ctgcatccgt ccggacattc agggcggcag cctgaccgcc tatttcgatc
     5641 ccgcccacct gcggcaaatt atgtggaacc tcgccaacaa cgcgtggcgg cacagccgca
     5701 aacagcccgg ctcgatttcc gtcaccatcc gccccgcgca aaaaaacacc gtctgcatcc
     5761 tctttgccga ccgcccgaag tgcaggaaca cctgttcgaa cccttttaca ccacggcgga
     5821 aaacggcacc ggcctcgggc tgtatgtcgc ccgcgaactg gcacacgcca acttcggcga
     5881 tttgacctac ctgccggaag ccaaatgttt caaactcaca ttaccggaaa aaagcaatga
     5941 ctgaactgca acatcccgtc ctcgtcgttg atgacgaaac cgatattctc gacctgatgg
     6001 aaatgaccct gatgaaaatg ggcttgcgcg tccataccgc gtcaggcgtt gccgaagcaa
     6061 aaaacaagct cgacagccaa cgctattcgc tcgtcctgac cgatatgcgt atgccggacg
     6121 gctcggggct agaagtcgtc caacacatca acagccgcct gctcgatacg ccggttgctg
     6181 tcatcaccgc cttcggcaat gtcgatcagg cacaggaagc cctgcgttgc ggcgcgttcg
     6241 accccgatac catgcagata caggactatc tcgaccaaat cgaacgcgac atcatcgaac
     6301 aaaccctcaa acaaaccgaa ggcaaccgca cgcaggccgc caaacgcttg ggcatcagct
     6361 tccgttcgat gcgctaccgt atggaacgcc tcaacatcgg ctgacgacaa aacggcatcc
     6421 gcaccatctc cgcccacccg aaaaaatgcc gtctgaaacg gcacggcaaa gcgggttcgc
     6481 cccacgcccg aacggacaca aaacaccatg accgacatcc tcattgacaa caccgccacc
     6541 gaaaccgtcc gcaccctgat acaggcattc ccccttgtgc ccgtttccca accgcccgaa
     6601 caaggcagtt acctccttgc tgaacacgat accgtcagcc tcaggcttgt cggggaaaaa
     6661 agcagcgtca tcgtcgattt tgcctccggc gcggcacaat accggcgcac aaaaggcggg
     6721 ggcgaactca tcgccaaagc cgtcaaccac accgcgcacc ccaccgtttg ggacgcaacc
     6781 gcaggattgg ggcgcgacag cttcgtcctc gcctcgctcg ggctggccgt taccgccttc
     6841 gagcaacatc ccgccgtcgc ctgcctgctt tcagacggca tccgccgcgc cctcctcaat
     6901 cccgaaacgc aagacaccgc cgcgcgcatc accctgcatt tcggcaacgc cgcagtacaa
     6961 atgcccgcac ttgttcaaac acaaggcaaa cccgacatcg tctatctcga ccccatgtat
     7021 cccgaacgcc gcaaaagtgc cgccgttaaa aaagaaatga cctacttcca ccggctcgtc
     7081 ggcgaagcgc aagatgaagc ggcactcctg cataccgcac gccaaacagc aaaaaaacgc
     7141 gtcgtcgtca aacgcccccg cctcggcgaa caccttgccg gacaagcccc tgcctaccaa
     7201 tacaccggca aaagcacccg cttcgacgtt tacctgccct acgggacgga caagggataa
     7261 cgcccataaa acaagacacc gaaaaatttg ccgttcttat gcaaacgaga aaccggtttt
     7321 tgcgtttcga ctgttttgga taaatcatca caccttaaag tt
//
LOCUS       DAWFAD010000099         7348 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWFAD010000099 DAWFAD010000000
VERSION     DAWFAD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.099"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            144..1154
                     /gene="dusB"
                     /locus_tag="WGO49_001494"
     CDS             144..1154
                     /gene="dusB"
                     /locus_tag="WGO49_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ0306593.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTKKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQVIDINMGCPA
                     KKVCNAQAGSALMQNEPLVAAILEAVVRAVDVPVTLKTRLGWHDDHKNLPAVARIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAAAVFKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIGEMPDGEQARREINRLDSAAAQYDMLAGYLERLAGKTDRWACGYRTN
                     AF"
     gene            1184..1423
                     /locus_tag="WGO49_001495"
     CDS             1184..1423
                     /locus_tag="WGO49_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ0306594.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            1426..1962
                     /gene="ruvC"
                     /locus_tag="WGO49_001496"
     CDS             1426..1962
                     /gene="ruvC"
                     /locus_tag="WGO49_001496"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0306595.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPPDAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            2013..2882
                     /locus_tag="WGO49_001497"
     CDS             2013..2882
                     /locus_tag="WGO49_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ0306596.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYLLVKPRRRIGEIN
                     LAKCFPEWDGKKRETVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDDAL
                     AAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDAQILKGRNRYDNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWESFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            3632..4426
                     /locus_tag="WGO49_001498"
     CDS             3632..4426
                     /locus_tag="WGO49_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0306597.1"
                     /translation="MNTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLIYGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSTQTEDKPHWSEFAQAWK"
     gene            4638..7241
                     /gene="pepN"
                     /locus_tag="WGO49_001499"
     CDS             4638..7241
                     /gene="pepN"
                     /locus_tag="WGO49_001499"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ0306598.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSTKLLSVKINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDCFTTMGGRNVKIEFYTAEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAESRL
                     KNNIFELTIKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
BASE COUNT         1789 a         1959 c         1973 g         1627 t
ORIGIN      
        1 ccgcctgtga gtggcggggc agggaatctg tgtgcggatt atgccataaa acggtgtccg
       61 ccccaatcgc gggcgcgtcc ggagattgga aatcctgctt aaaaaatgta caatggcgca
      121 cttttttgaa acgcggatcc attatgcaca tcggcggcta ttttatcgac aaccccatcg
      181 cacttgcacc gatggcgggc attaccgaca aacccttccg ccgcctctgc cgcgatttcg
      241 gcgcgggttg ggcggtgtgc gaaatgctga ccagcgaccc gacacttaga aataccaaaa
      301 agaccctgca ccgcagcgat tttgccgatg aaggcggcat cgttgccgtg cagattgccg
      361 gcagcgatcc gcagcagatg gcggatgccg cgcgttacaa cgtcagcctt ggggcgcagg
      421 ttatcgacat caacatgggc tgtcccgcca agaaagtgtg caatgcccaa gccggcagcg
      481 cgctgatgca gaacgagccg ctggttgccg ccattctcga agccgtcgtc cgtgcggtgg
      541 acgtacccgt taccctcaaa acccgtttgg gctggcacga cgaccacaaa aacctgcctg
      601 ccgtcgcccg catcgccgaa gattgcggca ttgccgccct tgccgtccac ggacgcactc
      661 gcacgcaaat gtacaaaggt gaagcggctt acgacctgat tgccgaaacc aaatgccgtc
      721 tgaacatccc ggtctgggtc aacggcgaca tcacttcgcc gcaaaaagcc gccgccgtct
      781 tcaaacaaac cgccgccgac ggcatcatga tagggcgcgg cgcgcaaggc agaccgtggt
      841 tcttccgcga tttgaaacat tacgccgaac acggtgtttt accgcctgcc ttgagtttgg
      901 cagaatgcag tgccgccatt ttgaaccacg tccgcgccat gcacgcgttt tatggtgaga
      961 ccgccggtgt gcgcattgca cgcaaacaca taggctggta catcggcgaa atgcccgacg
     1021 gcgaacaggc gcggcgtgaa atcaaccgct tggacagtgc ggcggcgcaa tacgacatgc
     1081 ttgcaggtta tcttgaaaga cttgccggaa aaaccgaccg ttgggcgtgc ggctaccgca
     1141 caaatgcctt ctgaacactt gattatcctt tgaaagtgca atcatgcccc atacccttcc
     1201 cgatatttcc caatgtatca gacaaaattt ggaacaatat ttcaaagacc tgaacggtac
     1261 cgaaccttgc ggcgtgtacg atatggtatt gcatcaggtg gaaaagccgc tgctggtgtg
     1321 cgtgatggag caatgcggcg gcaaccagtc caaagcctcc gtcatgttgg gactgaaccg
     1381 caatactttg cgtaaaaaac tgattcaaca cggtttgctg tgaatatgtc ggcaaccgtc
     1441 cgtatcttag gtatcgaccc gggcagccgc gtaacgggtt tcggcatcat cgatgtcagg
     1501 gggcgcgatc atttttacgt cgcctccggc tgcatcaaaa cgcctcccga tgcgcctctg
     1561 gcagacagga ttgccgtgat tgtgcggcac atcggcgaag tcgttgccgt ttacaagccg
     1621 cagcaggcgg cggtggaaca ggtgttcgtc aacgtcaatc cggcatcgac gctgatgctg
     1681 ggtcaggcta ggggcgcggc attggcggca ttggtcagcc ataagctgcc cgtttcggaa
     1741 tacacggcct tgcaggtcaa acaggcggta gtcggcaaag gcaaggcggc gaaagaacag
     1801 gtgcagcata tggtggtgca gatgctgggg ctttcgggaa cgccgcaggc ggatgcggcg
     1861 gacggtcttg ccgtcgcgct gacccacgcc ttacgcaacc acgggcttgc cgccaaactc
     1921 aatccttcgg ggatgcaggt caagcgcggc aggtttcaat agtttcagac ggcatttgta
     1981 ttttgccgtc tgaaaagaaa atgtgtatcg agatgaaatt tatatttttt gtactgtatg
     2041 ttttgcagtt tctgccgttt gcgctgctgc acaaacttgc cgacctgacg ggtttgctcg
     2101 cctacctttt ggtcaaaccc cgccgccgta tcggcgaaat caatttggca aaatgctttc
     2161 ccgagtggga cggaaaaaag cgcgaaaccg tattgaagca gcatttcaaa catatggcga
     2221 aactgatgct tgaatacggc ttatattggt acgcgcctgc cgggcgtttg aaatcgctgg
     2281 tgcgttaccg caataagcat tatttggacg acgcgctggc ggcgggggaa aaagtcatca
     2341 ttctgtaccc gcacttcacc gcgttcgaga tggcggtgta cgcgcttaat caggatgtac
     2401 cgctgatcag tatgtattcc caccaaaaaa acaagatatt ggacgcacag attttgaaag
     2461 gccgcaaccg ctacgacaat gtcttcctta tcgggcgcac cgaagggctg cgcgccctcg
     2521 tcaaacagtt ccgcaaaagc agcgcgccgt ttctgtatct gcccgatcag gatttcggac
     2581 gcaacgattc ggtttttgtg gattttttcg gtattcagac ggcaacgatt accggcttga
     2641 gccgcattgc cgcgcttgca aatgcaaaag tgatacccgc catccccgtc cgcgaggcgg
     2701 acaatacggt tacattgcat ttctacccgg cttgggaatc ctttccgagt gaagatgcgc
     2761 aggccgacgc gcagcgcatg aaccgtttta tcgaggaacg cgtgcgcgaa catcccgagc
     2821 agtatttttg gctgcacaag cgtttcaaaa cccgtccgga aggcagcccc gatttttact
     2881 gactacgtaa aattacaaaa catatcaggc gtttcaagtc gaaattcctg attgtttttg
     2941 gaaatttgaa atccgggtcg tgcaaacagg atttgccgga cggttttaac ggttcagttg
     3001 tttgtaaaaa caatgctttt taagattgac aaaaaaccag aatcaggttt tagggcttat
     3061 tccgcgaaca aagggggcgg atgccgaaaa tccgattaat atattaaaat taaatttgtt
     3121 taaaaacata aagttgtaaa caagtatctc atataagcct ttttcattaa acagatagtc
     3181 agatattttg tgctaaaaat ttatataata tttaaattaa tatcaagtta taaaaatata
     3241 tagaatttta ttttgtttat ttataatttt aagcaataat ctaaatcagg cattttgatt
     3301 cccctgtttt aaaaaatact tggcaaggtg tgaaaaaagg cgtacattcc gctacacaga
     3361 attacagacg cagcggagca atgccgtctg aaaggatttt ccggtcagtc ttgcgattgg
     3421 tcggggtttc atcggaaacg gtgaaacgaa agtttgccgg cgtagagttg agctacgcgg
     3481 gcaaagccgc aggcgaaagc ctgtattgtt tgtgaagcgt aaatctctga tttgaggtat
     3541 tggggcaatc ctgtgggggg ttgcctcttt ttttatccgc cttttaatga cacaataggc
     3601 gcaagtctgt ttttgaatgc aaggtactgc catgaatacg attttggctt tcgatattga
     3661 aaccgtaccc gatgtgcagg gtatccgaac attgtacgag ctgccgtcct cgctgcccga
     3721 cgatgaagtg gtgctgtttg cccagcaaaa acgccgtgct cagacgggtg gggattttat
     3781 gcagcatcat cttcatcaga ttgtggcgat ttcgtgctgc atgcgttggg ggcaggataa
     3841 agtccatgtc ggcaccatcg gcgagatgga cgatggcgaa gaagtcgtga ttgccaaatt
     3901 tttcgagttg attgaaaaac atacgccgca actggtcagt tggaacggcg gcggtttcga
     3961 cctgccggtt ctgcattacc gctccctgat atacggcatt aacgccgcgc gctattggga
     4021 tatgggcgac ggcgatttcg gcgacagccg cgatttcaag tggaacaact acatcagccg
     4081 ttatcaccaa cgccactgcg atttgatgga tttgctcgcg ctttaccagc cgagggcaaa
     4141 cgtgccgctg gacgatatgg cgaaactgtg cggttttccg ggcaagctgg gtatggacgg
     4201 cagcaaggtg tgggatgcgt tccacgcggg caggctgaaa gaaatccgca attattgcga
     4261 aaccgatgcc gtgaatacgt atttgatgta tctgcgtttt tgtctggtca gcggcagatt
     4321 cgacgcggac gaatacgaaa tggaaatcaa gcggatcaga aactatcttt ccacccaaac
     4381 agaagataaa ccgcattggt cagaatttgc ccaagcgtgg aaatagaaaa cctgctgtcc
     4441 gaaggcggta cgcttgtgcc gccgtttcgg tttcagacgg catatggtgg attaaattca
     4501 aaccggtaca gcccgtacta tctgtactgt ctgtggcttc gtcgccttgt cctgattttt
     4561 gttaatccgc tttaaaagct gtatccgtgc tgtctgaagc cttttccagt caaaccaaac
     4621 aatattgaaa gaaaaaaatg agcaaaaccg tccgttatct gaaagattac caaacgcctg
     4681 cctaccgcat ccttgaaacc gacctgcatt ttgatattgc cgaaccgcaa actgtcgtga
     4741 aatcccgttt gacggtcgag ccgcagaggg tgggggagcc gctggtgttg gacggttcga
     4801 cgaaactctt gtccgtcaaa atcaacggcg tggcggcgga ttatgtgttg gaaggcgaga
     4861 ctctgacgat tgcagacgtg ccgtccgaac gcttcaccgt cgaagtggaa accgagatcc
     4921 tgccggcgga aaacaaatcg ctgatggggc tgtatgcttc cggtggcaat ctgtttaccc
     4981 aatgcgagcc ggagggcttc cgcaaaatca cgttctacat cgaccgtccg gatgtgatgt
     5041 ccaaattcac gaccaccatc gttgcggaca aaaaacgcta tcccgttttg ctctccaacg
     5101 gcaacaaaat cgacggcggc gagtgttcag acggccgcca ttgggtgaaa tgggaagacc
     5161 cgtttgccaa accgagctat ctgtttgctt tggtcgcggg cgatttggcg gtcacggaag
     5221 actgtttcac cacgatgggc ggcagaaacg tcaaaatcga gttttacact gccgaggcgg
     5281 acaagcctaa ggtcggcttt gccgtggaat ccttgaaaaa cgcgatgaag tgggacgaaa
     5341 cgcgcttcgg tttggaatac gacttggata ttttcatggt cgtcgccgtg ggcgatttca
     5401 atatgggtgc gatggaaaac aaggggctga acatttttaa taccaagttc gtccttgccg
     5461 acagccgtac cgccaccgat accgattttg aaggcatcga atccgtggtc ggacacgagt
     5521 atttccacaa ctggacaggc aaccgcgtta cctgccgcga ttggttccag ctttcgctga
     5581 aagaagggtt gaccgtgttc cgcgaccaag aattttccgg cgaccgcgcc agccgcgccg
     5641 tgcgccgcat cgagaacatc cgcctgctgc gccagcacca gttccccgaa gacgcaggcc
     5701 cgaccgccca tccggtgcgc cccgccagct atgaggagat gaacaatttc tacaccatga
     5761 ccgtttatga aaaaggcgcg gaagtggtgc ggatgtatca caccttgctc ggcgaagagg
     5821 gcttccaaaa aggcatgaag ctctatttcc aacgacacga cgggcaggcc gtgacctgcg
     5881 acgatttccg cgcggcgatg gcggacgcga acggcatcaa tctcgaccag ttcgccttgt
     5941 ggtacagcca ggcgggcacg cccgttttgg aagcggaaag ccgtctgaaa aacaatattt
     6001 tcgagttgac cattaaacaa accgtgccgc ccacgcccga tatggcggac aagcagccga
     6061 tgatgattcc cgtcaaaatc gggctgctga accgcaacgg cgaagcggcg gcattcgatt
     6121 atcagggcaa acgcgcgacc gaagccgtat tgctgctgac cgaagccgaa cagatcttcc
     6181 tgctcgaagg cgtaaccgaa gccgtcgttc cctcgctgct gcgcgggttc agcgcgccgg
     6241 tgcatctgaa ctatccgtac agcgacgacg acctgctgct tctgctcgcc catgacagcg
     6301 acgccttcac gcgctgggaa gccgcacaaa cgctctaccg ccgtgccgtc gccgccaacc
     6361 ttgccgcact ttcagacggt attgaactgc cgaaacacga aaaactgctt gccgccgtcg
     6421 aaaaagtcat ttcagacgac ctcttagaca acgccttcaa agccctgctt ttgggcgtgc
     6481 cgtctgaagc cgagctgtgg gacggcgcgg aaaacatcga cccgctgcgc taccatcagg
     6541 cgcgcgaagc cttgttggac acgcttgccg tccgcttcct gccgaaatgg cacgaattga
     6601 accgtcaggc ggcgaagcag gaaaaccaaa gctacgagta cagccccgaa gctgccggct
     6661 ggcgcacgct gcgcaacgtc tgccgcgcct tcgtcctgcg cgccgacccc gcgcacatcg
     6721 aaaccgttgc cgaaaaatac ggcgaaatgg cgcaaaacat gacccacgaa tggggcatcc
     6781 tgtccgccgt caacggcaac gaaagcgata cgcgcaaccg cctgctggcg cagtttgccg
     6841 acaagttttc agacgacgcg ctggtgatgg acaaatattt cgcccttatc ggctcaagcc
     6901 gccgcagcga caccctgcaa caggttcaaa ccgccttgca gcatccgaaa ttcagcctcg
     6961 aaaaccccaa caaagcccgt tcgctcatcg gcagcttcag ccgcaacgtc ccgcatttcc
     7021 acgcagaaga cggcagcggc taccgcttca tcgccgacaa agtcatcgaa atcgaccgct
     7081 tcaacccgca ggtcgccgcc cgtttggtgc aggcgttcaa cctctgcaac aagctcgagc
     7141 cgcaccgcaa aaacttggtg aaacaagcat tgcagcgcat tcgggcgcag gaaggattgt
     7201 cgaaagacgt gggcgaaatc gtcggcaaaa ttttggattg aggccgtcaa acagaaaaac
     7261 aatgccgtct gatatagtta cacacacgtt ttatcatcac ttccccatcg ttttattacg
     7321 caatggcaaa acggccgcaa agtcatat
//
LOCUS       DAWFAD010000100         7204 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWFAD010000100 DAWFAD010000000
VERSION     DAWFAD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.100"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(64..148)
                     /locus_tag="WGO49_001500"
     tRNA            complement(64..148)
                     /locus_tag="WGO49_001500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(112..114),aa:Leu,seq:caa)
     gene            425..1078
                     /locus_tag="WGO49_001501"
     CDS             425..1078
                     /locus_tag="WGO49_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ0306599.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQVASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1157..1810
                     /locus_tag="WGO49_001502"
     CDS             1157..1810
                     /locus_tag="WGO49_001502"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ0306600.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1914..2813
                     /locus_tag="WGO49_001503"
     CDS             1914..2813
                     /locus_tag="WGO49_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ0306601.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2810..4099
                     /gene="hisD"
                     /locus_tag="WGO49_001504"
     CDS             2810..4099
                     /gene="hisD"
                     /locus_tag="WGO49_001504"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ0306602.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4145..5242
                     /locus_tag="WGO49_001505"
     CDS             4145..5242
                     /locus_tag="WGO49_001505"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ0306603.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5239..6156
                     /gene="hisB"
                     /locus_tag="WGO49_001506"
     CDS             5239..6156
                     /gene="hisB"
                     /locus_tag="WGO49_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ0306604.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6251..7120
                     /locus_tag="WGO49_001507"
     CDS             6251..7120
                     /locus_tag="WGO49_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0306605.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1880 a         2123 c         1723 g         1478 t
ORIGIN      
        1 ttagttttat ctatttcatt aaacagcaat agacaaaaaa aataaccgct ctaaaagcgg
       61 ttgtggtgcc cagggtcgga ctcgaaccga cacaccttgc ggcgggggat tttgagtccc
      121 ctgcgtctac caatttcgcc acctgggctg gtgaagaagt cgtcattata atggcttttg
      181 aaattctgta aacctttttt ttgaaattat tttatctgtt tttattttat ttttgatttt
      241 aaatagaatt tttattattt taatcttact gttctttccg ctccaaagat tctgtatgat
      301 tcggcaattc ctgccgtgca gacaacgtaa aaaaatacta cattaaatct gccaaacgcg
      361 ttaagatgga aatattcaaa ttccgtacga atcaggtttt gctatttatt cttgggagat
      421 tgtcatgttt tccgtaccgc gttccttttt gccgggcgtt ttcgtacttg ccgcgcttgc
      481 cgcctgcaaa cctcaagaca acagtgcggc gcaagtcgct tcttcaagtg catccgcgtc
      541 ggctgcggaa aatgcggcaa agccgcaaac gcgcggtacg gatatgcgta aggaagacat
      601 cggcggcgat ttcacgctga ccgacggcga aggcaagcct ttcagcctga gcgatttgaa
      661 aggcaaggtc gtgattctgt ctttcggctt tacgcactgt cccgatgtct gcccgacaga
      721 gcttttgacg tacagcgaca cgttgaagca gttgggcggg caggctaagg acgtgaaagt
      781 ggtgttcgtc agcatcgatc cggaacgcga cacgcctgaa atcatcggca agtatgccaa
      841 acagttcaat ccggacttta tcggtctgac ggcaacgggc gaccaaaacc tgccggtcat
      901 caagcagcaa taccgcgtgg tttctgccaa agtcaatcaa aaagacgaca gcgaaaacta
      961 tttggtcgac cactcttccg gtgcgtatct catcgacaaa aacggtgagg ttgccatttt
     1021 ctcgccttac ggaagcgagc cggaaacgat tgctgccgat gtaaggactc tgctctgata
     1081 aaaccgtatg ccgtctgcac cgtcggcgcc tattcagacg gcattattgt ttcaaccgac
     1141 aaaggacatc cacaccatgc aggataatgc tttgacaatc gccttatcca aggggcgcat
     1201 ttttgaggag acgctgccgc tgcttgccgc tgccggcatt gttccgactg aagagcctga
     1261 aaaatcgcgc aagctgatta tcgggacgaa ccatgaaaac atccgccttg tcattgtccg
     1321 cgcaaccgat gtgccgactt atgtccgcta cggcgcggcg gacttcggca ttgcgggcaa
     1381 agacgtgctg atcgaacacg gcggcacggg gctttaccgg cctttggatt tggagattgc
     1441 caagtgccgc atgatggttg ctgtgcgtaa agggtttgat tacgaagcag cttcgcaacc
     1501 cggatgccgt ctgaagattg ccacgaagta tcctgaaatc gcggcatctc attttgccgg
     1561 caagggtgtc catgtggaca ttatcaaact gtacggctcg atggaacttg cgccgctggt
     1621 cggcttgagc gatgcgattg tggacttggt ttcgacgggc aacaccttga aggcaaacgg
     1681 cttggaagca gtcgaacaca tcgtcgacat ttccagccgc ctggtggtca acaaggctgc
     1741 tttgaaaacg aaatacgcgc tgctggagcc gattattcag gcgttcggcg gcgcagtgaa
     1801 ggcgaagtaa gcatccattt gaataaagat gcgttttcag acgaccctat ccgttcccgc
     1861 cgacaggtcg tctgaaaata tcaccggcag taaactgtat aggagaagtt aaaatggttg
     1921 caaaaataaa aaaattctca gattcaaccc tttccgtttt gaataacggc gagcgtcggt
     1981 tttatgtcta ttgtctgacc gacctgaaaa aagacaaaat cctctacatc ggcaaaggct
     2041 gcggtaatcg tatcttcgag catgaatggg ttgctagtcg ttcacaagac ccagtctccg
     2101 gcgagattat cgatcggaaa ctcaaagcca tctccaaatg caagaaactc ggtcgctata
     2161 tcatcagcta tcatctgact gaagtcgaag cactcgccgc cgaatctgcc ttaattcatt
     2221 ttgttaaatc tgtcttgggt aaaaaactca aaaataaaat tgccgggcat ggtccgggtg
     2281 gtattagcgt agaagaacta gatcgccgct ttggattctc ttctctccca cttaacgaga
     2341 ttaaccccga cgggctgatt ctcgccatca aaatccacaa tgctttcgat ttagatactg
     2401 acgaagaatt agactacctt ttcgacaacc aagacgatgc caacctcaaa tcgcgtacgt
     2461 tgggcaactg ggttatcggt aaagatgttg cttcaaaagt gaaatacgtt atcggcgttc
     2521 acaccggtct gcaaaacgct gttgtcagtg catacgaagt ggacggtttt gaaacaatgg
     2581 ttgaggaaac caaaaacggt agaaaacaat cccgttaccg tttccgcact acctctcgta
     2641 gcgaagaggt attagccaaa ctcggtctgc aacaaaaatg cctgcccgaa ttgaagtttg
     2701 gtagcggggg agaaaaagcg tatatcagac ccaaaacaga gacagaaact gaacaagaga
     2761 atattcagac gacccccaat ccaaaaataa aaaaggaaaa aaccaaatca tgaaaaaact
     2821 caacacccaa tcgcccgatt tccaagccgg actcaaagcc ctgctggctt ttgaaaccgc
     2881 gcaaaacccc gaaaccgaac gcatcgtcgc cgacatttgc gccgacgtgc aaaagcgcgg
     2941 cgatgcggct ttgattgaat acaccaacaa attcgatcag acaaacgcta aaagcatcga
     3001 tgatttaata ctcacgcaag ccgatttgaa cgcggcgttc gagcgcattc cgaacgacgt
     3061 tcagacggca ttgcagaccg ccgcccgccg tgtcgaaagc taccaccaac gccaaaaaat
     3121 ggaatcgtgg agctacaccg atgaagacgg cacgctgttg ggacaacaga ttaccccgct
     3181 tgaccgcgtc ggcatttacg tccccggcgg caaggcggca tatccgagtt ccgtcatcat
     3241 gaacgccatg cccgcccacg tcgcaggcgt gaaagaaatc atcatggtcg tgccgacacc
     3301 gaaaggcgaa cgcaacgaca tcgtgcttgc cgccgcatac gtcgccggcg taaccaaagt
     3361 cttcaccgtc ggcggcgcgc aggcggttgc cgccctcgcc tacggcacgg aaaccatccc
     3421 ccaagtcgat aaaatcaccg gtccgggcaa cgccttcgtc gccgccgcca aacgccgcgt
     3481 gttcggcgtg gtcggcatcg acatggtggc ggggccgtct gaaatcctgg tcatcgccga
     3541 cggcacgaca cctgccgatt gggtggcgat ggatttgttc agccaggccg aacacgacga
     3601 aattgcccaa gccatcctca tcggcacgtc gcaagcgtat ctcgacgaag tagaagccgc
     3661 tatggaccgc ctgatcgaaa ctatgccgcg ccgcgacatc atcgaagcct cgctcggcaa
     3721 caggggcgcg atgatactcg ccaaagactt ggacgaagcc tgcgaaatcg ccaactacat
     3781 ttcccccgaa cacttggaac tgtcagtcga aaatccgcag gaatgggcga aaaaaatccg
     3841 ccacgccggt gcgattttca tgggacgcta caccggcgaa agcctcggcg actactgcgc
     3901 cggtccaaac catgtattgc ccaccagccg aaccgcccgc ttttcctcgc ctttggggac
     3961 atatgatttc caaaaacgct ccagcctgat tcaggtttcg gaacagggcg cgcaaaaatt
     4021 aggcgaaacc gccagcgtgc tggcacacgg cgaaagcctg accgcccacg cccgcgcggc
     4081 agagttccgt atgaaataat gccgaaacgg cgtacaggca tattccaacc attaaggaaa
     4141 cacgatgaaa tccgtccgct ccttcatccg cgacgacata caagctatgt cggcatatca
     4201 gattgccgac gttccgcccg gctttgccaa actcgattcg atggaaagtc ccgtccaccc
     4261 ttttgccgga catgaaacgc tgttgcagga atggcaggca cggcttgccg ccgcgcccat
     4321 ccatctttac cccaatccct ccggcagcgg tttacaggaa gcattacgtt cggcgttcga
     4381 cattcccgac tgcgccgaca tcgcgctggg caacggctcg gacgagctga tacagttcat
     4441 cacgatgctg accgccaaac cgggcgcggc aatgttggca gccgaaccca gtttcgtcat
     4501 gtaccgccac aacgccgcgc tgtacggcat ggattatgtc ggcgttccac tgaacggaga
     4561 tttcaccctc aacctgcccg ccgtcctcga agccgtcagg aagcaccgcc ctgccctgac
     4621 ctttatcgcc taccccaaca accccaccgg cgtatgtttc acgcgtgccg aaatcgaagc
     4681 cgtcatcgaa gcttcagacg gcatcgtcgt cgtcgacgaa gcctacggcg cattcaacgg
     4741 cgacagcttc ctgccgcagg cgggcagcat tcccaacctc atcgtcatgc gtaccgtcag
     4801 caaaatcggt tttgccggac tgcgtatcgg ctatgcggca ggctgtcccg aagtcatcgg
     4861 cgaactgcaa aaaatcctgc cgccctacaa tatgaaccaa ctgagcctga ccaccgccaa
     4921 actcgccctg caacactacg gcatcatctc tgccaacatc gacagcctga aaaacgaacg
     4981 cgaacggatg ttcgccgaat tgggcaaaat atgccgtctg aacacctttc caagtcaggc
     5041 aaacttcatt accatacgcg tacccgatgc cgatttgttg tttgacacgc tcaaacaaaa
     5101 ccgcatcttg gttaaaaaac tgcatggcgc gcacccgctt ttggaacact gcctgcgcat
     5161 taccgtaggc agccccgcac aaaacgatgc cgttctcaac atcattcgcc aactttactg
     5221 ccaaccaacg gatttcctat gaatttgact aaaacacaac gccaactgca caactttctg
     5281 accctcgccc aagaagcagg ttcgctgtcc aagctcgcca aactctgcgg ctaccgtacc
     5341 cccgtcgcac tctacaaact caaacaacgc cttgaaaagc aggcagaaga cccagatgca
     5401 cgcggcatcc gtcccagcct gatggcaaaa ctcgaaaaac acaccggcaa acccaaaggc
     5461 tggctcgaca gaaaacaccg cgaacgcact gtccccgaaa ccgccgcaga aagcaccgga
     5521 actgccgaaa cccaaattgc cgaaaccgca tctgctgccg gctgccgcag cgttaccgtc
     5581 aaccgcaata cctgcgaaac ccaaatcacc gtctccatca acctcgacgg cagcggcaaa
     5641 agcaggctgg ataccggcgt acccttcctc gaacacatga tcgatcaaat cgcccgccac
     5701 ggcatgattg acatcgacat cagctgcaaa ggcgacctgc acatcgacga ccaccacacc
     5761 gccgaagaca tcggcatcac actcggacaa gcaatccggc aggcactcgg cgacaaaaaa
     5821 ggcatccgcc gttacggaca ttcctacgtc ccgctcgacg aagccctcag ccgcgtcgtc
     5881 atcgaccttt ccggccgccc cggactcgtg tacaacatcg aatttacccg cgcactaatc
     5941 ggacgtttcg atgtcgattt gtttgaagaa tttttccacg gcatcgtcaa ccacagtatg
     6001 atgaccctgc acatcgacaa cctcagcggc aaaaacgccc accatcaggc ggaaaccgta
     6061 ttcaaagcct tcgggcgcgc cctgcgtatg gcagtcgaac acgacccgcg catggcagga
     6121 cagaccccct cgaccaaagg cacgctgacc gcataaaaaa ccataccgtc tgaaacaccc
     6181 gcaggctttt cagacggtat cggaacagat aagattacac tacactacaa acagaaaagg
     6241 agtaaacatc atgtccgcaa acgaatacac acaaatcggc tggataggct tagggcaaat
     6301 gggtctgcct atggtaacgc ggctcttgga cggcggcatc gaagtcggcg tatacaaccg
     6361 ctcgcccgac aaaactgccc ccatctccgc caaaggcgca aaagtttacg gcaacaccgc
     6421 cgaactcgtc cgcgactatc ccgtcatttt cctgatggtt tccgactatg ccgccgtgtg
     6481 cgacatcctg aacggagtcc gcgacggatt ggccggcaaa atcatcgtca acatgagcac
     6541 catctccccg accgaaaacc tcgccgtcaa agcacttgtc gaagccgcag gcggacagtt
     6601 tgccgaagca cccgtttccg gatcggtcgg gcccgccacc aacggcacgc tgctgattct
     6661 gttcggcggc agcgaagccg ttttaaaccc gctgcaaaaa atattttccc tcgtcggcaa
     6721 aaaaaccttc catttcggcg atgtcggcaa aggttcgggc gcgaaactcg tcttgaactc
     6781 gctcttgggc attttcggcg aagcgtacag cgaagcgatg ctgatggcgc ggcagttcgg
     6841 catcgatacc gacaccatcg tcgaagccat cggcggctcg gcaatggact cgcccatgtt
     6901 ccaaaccaaa aaatccctgt gggcaaaccg cgaattccca cccgccttcg ccctcaaaca
     6961 cgcctccaaa gacctcaacc tcgccgtcaa agagcttgaa caggcaggca acaccctgcc
     7021 cgccgtcgaa accgttgctg ccagctaccg caaagcagtc gaagccggct acggcgaaca
     7081 ggacgtttcc ggcgtttacc tgaaattggc agaacactga ttgccttttc caaacacaat
     7141 gccgtctgaa catatttcag acggcatttt tatcacccca cgcttaaaat cagtcccgat
     7201 tatg
//
LOCUS       DAWFAD010000101         7119 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWFAD010000101 DAWFAD010000000
VERSION     DAWFAD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.101"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..139
                     /locus_tag="WGO49_001508"
     CDS             <1..139
                     /locus_tag="WGO49_001508"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0306606.1"
                     /translation="LQVRIGQRFYRTTARAVIGSGGTAEIPAIADEPGAAANVATARR
                     N"
     gene            139..750
                     /locus_tag="WGO49_001509"
     CDS             139..750
                     /locus_tag="WGO49_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0306607.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLKIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVPSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            747..1307
                     /locus_tag="WGO49_001510"
     CDS             747..1307
                     /locus_tag="WGO49_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ0306608.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            1318..3291
                     /locus_tag="WGO49_001511"
     CDS             1318..3291
                     /locus_tag="WGO49_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ0306609.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLSTAVGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGMTAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDTA
                     AVGYEDHNERQRSALVSTWTDGDLNDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRDGLLEALQD"
     gene            complement(3996..4307)
                     /locus_tag="WGO49_001512"
     CDS             complement(3996..4307)
                     /locus_tag="WGO49_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HEZ0306610.1"
                     /translation="MKMHNPAHPGLVLKEYINGASITDIAKRLGVSRVALSRIVNAQS
                     SITPEMAVRLSQLLNTSPDFWLNMQAGYDLWQIRQRKTFDIAPLFPEPPLSSMQTGAR
                     A"
     gene            complement(4315..4593)
                     /locus_tag="WGO49_001513"
     CDS             complement(4315..4593)
                     /locus_tag="WGO49_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0306611.1"
                     /translation="MIVSFKHKGLERFFKTGSLSGIQAGHSVKLNLLLTALNAAQTPS
                     DMAVPSWNLHPLKGSLSGHWAVKVNGNWRLTFRFNDGNAEVVDYQDYH"
     gene            4678..5283
                     /locus_tag="WGO49_001514"
     CDS             4678..5283
                     /locus_tag="WGO49_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306612.1"
                     /translation="MTIYFKNGFYDDTLGSIPEGAVAVRAEEYAALLAGQTQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAAARGVELDVLIEKVVEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            5262..5558
                     /locus_tag="WGO49_001515"
     CDS             5262..5558
                     /locus_tag="WGO49_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HEZ0306613.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            5555..5764
                     /locus_tag="WGO49_001516"
     CDS             5555..5764
                     /locus_tag="WGO49_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306614.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(5874..7017)
                     /locus_tag="WGO49_001517"
                     /pseudo
     CDS             complement(5874..7017)
                     /locus_tag="WGO49_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
BASE COUNT         1793 a         2082 c         1946 g         1298 t
ORIGIN      
        1 cctgcaagtg cgtatcggcc agcgttttta ccgcactacc gcccgcgccg ttatcggcag
       61 cggcggcacg gcggaaatac cggcaatcgc cgacgagccg ggcgcggccg ccaatgtggc
      121 gacggcgagg cgcaactgat ggccgccccc gccggtgtgg ccaccgaatg ccgccttacc
      181 gtacaaggcg gcaccgaccg agaaagcgat gcctcactgc tggcgcgtct gttgaaaatc
      241 atccgccgac cgcccgcagg cggcaaccgt tacgactata aaaactgggc gttgagtgtt
      301 gacggcgtaa ccagcgcata tgtttatccg ctgcgccgcg gcttgggtac ggtggatatt
      361 gccattacct ccgccgacgg tgtgccatcg gaagaaactg tgcgccgcgt acaggcttat
      421 atcgacgaga tgcgcccggt aacggcaaaa aatgcgctgg tactcaagcc aaccgtaacg
      481 gcggtgcctg ttaccgtgca agtcaagctc gacggcatcg acttggacga ggccaagcgc
      541 cgcatacgga cggccctaaa agaatatttc gacaccctga tccccggcga cggcctgact
      601 gtgtcgcaaa tcgaggcggc tatcagcaat gtggatggtg tgatcgaccg ccgtctgact
      661 gcgccgacgg ccaaccgtgc cgccgatacg gttaaccgca tcgagtggtt taaagcgggc
      721 gcgattaatg taacggagat gccgtcatga gctatcaaga catcttgcgg ggtctgttgc
      781 cccccgtgtc gtatgcccgc aatgccccgc gtgtgcgggc gcaggcagaa atagacggcg
      841 cagcgctgga tgcggtagcg gaatcggctc aaagcgttgc cgatgccgtc gacccgcgca
      901 gcgccggcca aatgctggcc gattgggagc gcgtattagg tttggacggt acgggcaaaa
      961 accgccagcg ccgtgtgttg gccgtcatgg ccaagctaaa cgaaacaggc ggcttgagta
     1021 ttccttattt tgtgcgtttg gccgaggcgg cgggctatca aatccaaatc gacgaaccgc
     1081 agccgttccg cgccggtgtc aaccgtgcgg gcgaccgtct tgcgccgcag gaaatcatgt
     1141 gggtgtggca cgttaacgtg cgcggcggca acaaccgcat tacccgattc cgcgccggta
     1201 tctcggcggc gggcgacagg ctgaccgatt acagcgatgc cgtgatcgaa agcctgttca
     1261 accgcctcaa gcccgcccac accgctatcc gatttaccta ccgataagga ctaaaccatg
     1321 caccccatcg aaacccccga taagaccttc cacgacggcg acggcgtgtc cgaattgggc
     1381 accatcctgc ccgcgtggtg gctcaaccaa gtgcaatccg agctgctggc cgtgctgact
     1441 gcggccggta tccagccgga taagtcgcag cctaatcaat tactggcagc actgaataga
     1501 ctggctgtag ttaccaccgg caaccaagaa atcggcggcc aaaaaacctt taccgcccaa
     1561 acccaattcc aaagcggcat ccatttatcc gccaaccaga cgaactggaa cggcggccac
     1621 aaagcctaca tcggcgcgga tgccgacaac gcccacatcg tcttcggcga cgacaccctg
     1681 cgcctgcacg gcgcaaacaa ccgcatttcc tacaacaacc acgacatctt ccacaaagcc
     1741 aacaaaccgc gttttgccga agacatcgaa ggcaaaccga acacactgtc cggatacggc
     1801 atcggcaatt tcaaagtcga aaccttccgg ggcgatttga acaccctcaa aacagacggc
     1861 atctattccc tgtcgacggc ggtcggcagc tccaacctgc ccgttgaaaa caccgcctgc
     1921 catatccaag tcatcgccgg cacgaaacac ggctggtgca ggcagttggg ttatcccgcc
     1981 tacacgtccg acgtgtacga acgccaccaa acgagcagcg caaacgacaa ctggtccgca
     2041 tggaaaaaac tcaattcgga cggcatcccc gtcggcgcga tcgtatcctt tcccaaagcc
     2101 gtccgaaacc ccgcaggcta tctcaaagcc aacggcacga cctttgcaca aaacaccttc
     2161 cccgacctct accgcgcctt gggcaacagc aaccgcctgc ccgatttaag ccgtaccgac
     2221 atcggcatga ccgcgtggtt tccgtccgac caaatcccga ccggctggct ggcgtttgac
     2281 gacatccgca cgcgcgtaac cgaaaccgct tatcccgagc tgtaccgtct gctgaccgga
     2341 aaatacggca gcatccaaaa cgtcccgcag gcggaagacc gctttatccg caacgcaggc
     2401 aacagcttgg cagtcggaac gaagcaggaa gacgaaatca aacggcacgt ccacaaagta
     2461 ttttcacact ggacaaacca cacagacacg gcagccgtcg gttacgaaga ccacaacgaa
     2521 aggcagagaa gcgcgctcgt atcgacttgg acggacggcg atttaaacga caacggcttt
     2581 ttaaccccgc gcccggacag caaaatggcg acaggcggcg acgaaaaccg ccccaaagcc
     2641 ctggttttaa aactgtgcat caaagccgcc gacaccttgg gcgaagccgt gttttggata
     2701 aagtcccacg gcgaaaccat caacgccggc gcgctggacg cgggcacgct ggcgcaaaac
     2761 ctccaagaca aagcagaccg cgcccacacc cacaccgccg cccaaatcca agggctggac
     2821 gaaaaaatca gcaccgccgt tgccgcgcaa ttcacacgcc aaaccatcgg cggcgtggat
     2881 attgtcagat tccccgacgg cacaatgata cagaccggca gctacaggtt cacacgaagc
     2941 ggcggcccca tcgaaaacga agtcgtcttc cccgtcgcct ttgccgacgg caacgtcaaa
     3001 tgcttcgtat ccgaacgcca ttcgggacgc gttaccggcg atcgaaggca acacaactgg
     3061 ctgtttatcc gcgcaaaaaa ccacgccgcc gccattatta ccaactggta cgaaggcagt
     3121 tgcgactgga tggccatcgg caaagccgcc tcgggaaacg ccgccagctc cccgataggc
     3181 cccgaaatac ctgaaaccaa cgaagaaccg caaagagaga gtggaagaac atcaaccgga
     3241 ccccgaaacc gccgccgccg agacggcttg ctcgaggcac tgcaagacta gcgggctgta
     3301 gagatggctg tagagacggg ctgtagagac gggctgtaga gatggctgta gagatggctg
     3361 tagagacggg ctgtagagat ggctgtagag acgggctgta gagatggctg tagagatggc
     3421 tgtagagacg ggctgtagag atggctgtag agacgggctg tagagacggg ctgtagagac
     3481 gggctgtaga gatgggctgt agagatgggc tgtagagatg ggcttcagcc cgccgatcca
     3541 agcaatccga ccgaaaccgg ccgcgccgcc aatcccccga aacctatgcc ccgccaatcc
     3601 tgccactctt cgtcattccc gccgctttcg tcattcccgc gaaagcggga atccagcccc
     3661 taaacgcggc aggaatctat cggaaaaaac agaaaccccc gccaccgtca ttcccgcgca
     3721 ggcgggaatc cagaccccaa acgcggcagg aatctatcgg aaacggctga aaccgaacgg
     3781 actggattcc cgcctgcgcg ggaatgacgg cggcaggggt ttcgggattc ccgccttcgc
     3841 gggaatgacg gaaagtggcg ggaataacga aaggcgggaa tgaccgcgca aaaagccgct
     3901 gcccccttcg gacggcaccg gcaacaaaaa accgcacggc cgaaaccgcg cgggaaagga
     3961 tagtcgggcg cgcccgataa gcagcggccg cagggttaag cccttgcacc tgtttgcatc
     4021 gaagacaaag gcggttcggg aaataagggc gcaatatcaa aagttttgcg ttgcctgatt
     4081 tgccacaaat cataaccggc ctgcatattc agccaaaaat caggcgaagt attgagcagt
     4141 tggcttaaac gaaccgccat ctcgggcgta atcgaagatt gggcgttgac aatgcgcgat
     4201 aaggcaaccc ggctgacacc caggcgtttc gctatatcgg taatgcttgc accgttaata
     4261 tactctttta aaacaaggcc cgggtgggca ggattgtgca ttttcatatt attcctagtg
     4321 ataatcctga taatcgacaa cttcggcatt gccgtcgttg aaacgaaaag tcaaacgcca
     4381 atttccgttg actttaaccg cccaatgtcc cgacaaactg cccttgaggg gatgaagatt
     4441 ccaactcggc acagccatat ccgaaggcgt ttgcgcggca ttcagcgcgg ttagaagcag
     4501 attcagtttg acggaatgcc cggcttggat gcccgatagc gagcctgttt taaaaaagcg
     4561 ttcaagccct ttgtgtttga aagaaacaat catcatccca ccccgtctgt atcgccaaag
     4621 gatacaaaac ttttatttaa ttgtaaagcg acgatataca aaaaaggaaa ccccgaaatg
     4681 accatctatt tcaaaaacgg cttttacgac gacacattgg gcagcatccc cgaaggcgcg
     4741 gttgccgtcc gcgccgaaga atacgccgcc cttttggcag gacagacgca gggcgggcag
     4801 attgccgcag attccgacgg ccgccccgtt ttaaccccgc cgcgcccgtc cgaataccac
     4861 gaatgggacg gcaagaaatg ggaaatcggc gaagccgctg ccgccgcccg tttcgccgaa
     4921 caaaaaaccg ccacggcatt ccgcctcgcg gcaaaggcgg acgaactcaa aaacagcctc
     4981 ttggcgggct atccccaagt ggaaatcgac agcttttaca ggcaggaaaa agaagccctc
     5041 gcgcggcagg cggacaacaa cgccccgacc ccgatgctgg cgcaaatcgc cgccgcaagg
     5101 ggcgtggaat tggacgtttt gattgaaaaa gttgtcgaaa aatccgcccg cctggccgtt
     5161 gccgccggcg cgattatcgg aaagcggcag cagctcgaag acaaattgaa caccatcgaa
     5221 accgcgccag gattggacgc gctggaaaag gaaatcgaag aatggacgct aaacatcggc
     5281 tgaaaaaata cgtttaccac ctgttggtag ccatcgacca actgttcaac gccctaaccg
     5341 gcggcgcggc ggacgaaacc ctctcaagcc gcacctatcg cggcgcgcgg ctcgcccaaa
     5401 agcccaaaac ccgctggaag gttttataca ccctgatcaa cggcgtgttt ttcgaccgcc
     5461 aacactgtcg gcaggcgtat atcagcgaac tgaaaggcag gcagcacgac gcgcggttca
     5521 accaaagccg cgccgccggg gaaaagggga cgcgatgaac tacttcggca tggtagagtt
     5581 tctgcgcctg atggcaatgg tgcggccgcc atttgtcttc ttctccggca cccgcagcga
     5641 actgcctact tatcttgacc tcgtggcaga actgcgcctg accggatggg agcgttttgc
     5701 aggcagccaa accttgactg tgagcagcac atcaacagca attcaagcta cgacgaccac
     5761 ctgatttata agttctgacc gcaagtagcg tactactttt aaaggcataa gataatcccc
     5821 gtttaacaga ccattaaacg gggataaatt tgtgcaaaag ctaatacaat ttcctacgct
     5881 tcggcggtgc aaaagctgcc gccaattcgt gcaaaagctg ccgccgcctt acattccagt
     5941 gcaatgccgt ctgaaacttc gctaatctcg ggttgccgcg cgctgtgttg ttcttcggta
     6001 ctcagcaaaa agccgtacag acgctccaat cgggcgcggt aggcggtgaa tttgttgtag
     6061 aacatccgga aaaaagatag cgcgttttgc agtcgcgcaa aggcttggac ggtctgctgg
     6121 atgtcgccga ttttgatttg tccggcaaac aggcgcggcg cttgcaaaat aatggggaag
     6181 agtttgatgc cgttggtgaa catatcgtta aagccgctta agcagacgct ttgccgcgcg
     6241 atgcgccaac ggttgcggat aatggcttta aagcggtcgg aaagccggcc gtgttcgtgt
     6301 tgttcgccgt tgtaaaacgc tacgctttcg gcgtggtcgc gtacgcggat gagggaataa
     6361 cggtagtcgc cgttgagttt ttcgttttca tagttgtaac gaatcaaggg gttgcctatc
     6421 cacatcgcga taaaggtcgc caaaatcaca aaaataaaga cgagccaaac aatgccgcgc
     6481 ggaatgtcga agccgaacac ggtcaggatg ccagccaagc cccacaaaac aacggcaaat
     6541 tccaaagagg taacgaccga attgaccatg ccgcgtacaa attcgatggt cgaagcgatg
     6601 aaatcctgcg cgtcctgttg gatacgttgg tcgatgttgt ccggcgcgtg gcggcgcatt
     6661 tgcaggcggt agtagttttt gtcggcaagc cagcgcgtcg tcagcacttc attgagctgc
     6721 tccgaccatt taatcgccaa gccttgatcg aggaagtcgt tgacgacatt gttaaacgcc
     6781 cgtatcagca ccacgcccgc gttcattgcg gcaaacatcc aaaatgccga agcatttcaa
     6841 atcctgcatc gagtcgtaaa gccctttgga cataaaggta ctcaacacat tcagccgcat
     6901 ttcggttaac accagcgtaa tcatcgccgt aatcagcagc aagactttga ccgcgctttt
     6961 cggtgtcaga caaagccaaa gcggtgtgga ataaagctcg gtttgccatt tctgcatggg
     7021 aaatttctta cggtatcaat gccgtctgaa aaagacgggt acagttgatt ttttgatgaa
     7081 gtttggggaa gttttgccgg tcagggtaca ttgcgtgtt
//
LOCUS       DAWFAD010000102         7088 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWFAD010000102 DAWFAD010000000
VERSION     DAWFAD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.102"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(66..425)
                     /gene="rnpB"
                     /locus_tag="WGO49_001518"
     ncRNA           complement(66..425)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WGO49_001518"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(504..1607)
                     /gene="prfB"
                     /locus_tag="WGO49_001519"
     CDS             complement(504..1607)
                     /gene="prfB"
                     /locus_tag="WGO49_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ0306615.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1710..2603)
                     /locus_tag="WGO49_001520"
     CDS             complement(1710..2603)
                     /locus_tag="WGO49_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ0306616.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2945..3394
                     /locus_tag="WGO49_001521"
     CDS             2945..3394
                     /locus_tag="WGO49_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0306617.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3468..3776
                     /locus_tag="WGO49_001522"
     CDS             3468..3776
                     /locus_tag="WGO49_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306618.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3892..4734
                     /locus_tag="WGO49_001523"
     CDS             3892..4734
                     /locus_tag="WGO49_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ0306619.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4724..5470
                     /locus_tag="WGO49_001524"
     CDS             4724..5470
                     /locus_tag="WGO49_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ0306620.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLLDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5492..6079
                     /locus_tag="WGO49_001525"
     CDS             5492..6079
                     /locus_tag="WGO49_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ0306621.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6085..6264
                     /locus_tag="WGO49_001526"
     CDS             6085..6264
                     /locus_tag="WGO49_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ0306622.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6496..6936
                     /locus_tag="WGO49_001527"
     CDS             6496..6936
                     /locus_tag="WGO49_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306623.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1808 a         1884 c         1615 g         1781 t
ORIGIN      
        1 caacctaaat aaaaatgccg tctgaaccat atttcaggtt tcagacggca tttgcgtgtc
       61 ggatgcacac cggacaggcg gtaagccggg ttctgtctcg gacagtcatt cctctaggca
      121 taccgttacc agtatgctca agcaacctac ccgaacgctc ggcgggcagc gtcattgcgt
      181 tctgtttggt cttgctccga atggggtttg gcctgccgca tattgttacc aaatgcgcgg
      241 tgcgccctta ccgcaccttt tcacccttgc ctgtgctgcc aaagcagcca tcggcggttt
      301 tgctttctgt tccactttcc gtcgcgttac cgcgcccggc cgttaaccgg cattctaccc
      361 tgcggagccc ggactttcct ccccgtatgc cttacgcgat acgcggcgac tgtctgctcg
      421 tcccgtgtgc ggcgcggatt ataacacgaa acgcaaaaat gccgtctgaa acggtacagg
      481 tttcagacgg catacagcct aaactacacg ccctgtttca ggctggcttc gataaagccg
      541 tccaaatcgc catccaatac ggctttggtg ttgccgactt cgtagcctgt acgcaagtct
      601 ttgatacgtg aggaatccaa aacatacgaa cggatttggc taccccaacc tacatcggat
      661 ttaccttctt ccaacgcctg tttctcttca ttgcgtttgc gcatttccaa ttcatacagt
      721 ttggacttca acatttccat cgcggcggct ttgttggcgt gttgcgaacg gtcgttttga
      781 cattgcacca caatccccgt cggctcgtgg gtaatacgca cggcggagtc ggttttgttg
      841 atgtgctgac cgcccgcgcc cgatgcgcga taggtgtcga tacgcaaatc ggcggggttg
      901 atttcgattt cgatggaatc atcgatttca gggtaaacga acacggaggc aaacgaagta
      961 tggcgtttgt tgttcgagtc aaacggtgaa tagcgcacca agcggtgaac gccagtttcg
     1021 gtacgcagca aaccataggc gtattcgcct tccactcgga tagtcgcgcg gttgatgccc
     1081 gcgatttcgc cgtcgtcttc ttcgaggatt tcgattttga agcctttgcg ctcggcgtag
     1141 cggctgtaca tgcggaacag catacccgcc cagtcttccg cttccgtacc gcccgcgcct
     1201 gcggtgatgt cgataaagca gttgttcggg tcggcgggct ggttgaacat ccgtttgaac
     1261 tccaaatccg ccatctgttt ttccagcccc gccacatctt cctgcacggc ggcaaaacct
     1321 tcttcgtcgt tttcttcgac ggtcatttca atcagcatgc ggttgtcttc gatgcccgaa
     1381 gcgatgttgt cgagcgtcaa cacgatgcct tcgaggattt tgcgctcttt gccgatttct
     1441 tgggcgcgtt tcgggtcgtt ccaaagttcg gggtcttcgg aaagaccgat aacttcttcc
     1501 aatcggtctt tcttaccctg ataatccata taaactcgga tgtcttcgct gcgcttttcc
     1561 aaatcattca gggtattgtt gagctggttg attacttcgg cttccatgat tcttttgttc
     1621 tttcaaaatt ttaggggcgt attgtacggg attcgggtat ttttttctat ggataaagcc
     1681 ttctagaaac acgttcggac ggcatagcgt caataacggt atgccgccag tttgcgtttg
     1741 atttcaggca atgcggcacg tgctgcctcc tcacccaact ggatggcacg ttttttctga
     1801 tcgaatccgc cgactgcacc caaatccaaa acctgcggtt tgataaccac atccgcctgc
     1861 cccaactcat tttgcaacgc ggaaacgctc attacgttca gcgtctgatc gagataagag
     1921 aagaagcctt ggctgatgtt tttgcccgga cgggcggaaa tatcgacggc aatcacgaaa
     1981 ttcgccccct gccgccgggc ggcactgacg ggcacgggct gcgacagacc gccgtcaaca
     2041 tatgtatgcc tgccgatgat aacgggttgg aacacattgg gaatggcggc ggaagcgcgc
     2101 acagcctgcc cggcattccc ttgattgaaa gcgacggcct tgccggtttc aaaatcagta
     2161 gcaacagcgg caaatttgat gggaaactgc tgaatctgcc tgccgccgac ttttcggttg
     2221 atgtaatttt gcagcttttc gcctttgata aaaccactgg tggacaaggt taaatcgact
     2281 aaatcggttt tgcctaaaat ttcggcttcc aattcgaggc ggtcgggcga catacccgat
     2341 gcaaaaaggc tgccgacaat cgaacctgcc gatgtgccgg taaccacctt cacaggaata
     2401 ccgttttctt tcaaaacctt aataatacct acatgggcaa atcctttaga tgcgccgcca
     2461 ccgagtgcca aaccgaccac tgcggcgggt ttggcggttt gcaccggctt gcgggcggca
     2521 ttatttcccg ccgtgccgca ggcggcaagc aacgcggcgg cggcgattgc caaaagcggt
     2581 ctgatttttg aaaacgttac catattttcc attcctttat atatcgcacc ccgtcaaaaa
     2641 gagggattgc ttttcttaac aacccccttt gacagccaag caaatggagg ctttgttaag
     2701 tcatcatcaa aattaatatt tctttttttc ctttacggaa attatatttg aaggcatact
     2761 atccaaggtg ggaattatct cacaacaccg ccgttatcca aatatcccgc ctttttccct
     2821 tcctttccat caaaatactt tctttttata ttcattaact tgttaaatca ttggctgccg
     2881 ggtgtcagtt tttccgacaa aatccgtcta atggggtatc aacagaacca aaacaggaac
     2941 acttatgaaa atcggaacaa cttggcagac ggcatccgct atgctggttt tgcgtctgtt
     3001 tgccgcatat gaatttttgg aatcgggttt gcaaaaatgg aacggggaga attggttttc
     3061 tgaaatcaac gatcaattcc cattcccgtt caacttgctg ccggacgcgt taaactggaa
     3121 tctcgctatg tatgcggagc ttttactgcc cgtactgttg cttttgggtt tggcaacgcg
     3181 tctgtcggca ttagggctga tggtcgttac cgccgtcgct tgggctgcgg ttcacgccgg
     3241 ttcgggttac aatgtctgcg acaacggtta taaaatggct ttaatttata tcgtggtatt
     3301 aatcccgctg cttttccagg gtgcgggcgg atggtcgctg gatacgctgc tgaaaaaacg
     3361 gttttgcccc caatgccgtc tgaagcaaga ttgattcagt cgtggaatct gactttaaac
     3421 attccaacct tatctcgtta acttgatatt ctgaaaagga aatagaaatg aacaaaaaca
     3481 ttgctgccgc actcgccggt gctttatccc tgtctttggc cgccggtgca gttgctgcca
     3541 acaaaccggc aagcaacgca acaggcgttc ataaatccgc ccatggctct tgcggcgcgt
     3601 ccaaatctgc cgaaggttcg tgcggcgcgg ctggttctaa agcaggcgaa ggcaaatgcg
     3661 gcgagggcaa atgcggtgcg accgtaaaaa aaacccacaa acacaccaaa gcatctaaag
     3721 ccaaggccaa atctgccgaa ggcaaatgcg gcgaaggcaa atgcggttct aaataatccc
     3781 accccttcaa accaagccgc gtttttcagt aaaatgcggc ttttttaacg gcaaacaaag
     3841 attttttaac aagcacatca ttcttttgtg ccatccgaac cgggtaaaaa tatgattcaa
     3901 cacgcaggct tgggctaccg ccgcgacttg gcggaagact ttctctcgct ttccgaaaac
     3961 agcccgatat gctttatcga agccgcaccg gaaaactggc tgaaaatggg cggctgggcg
     4021 cgcaaacagt ttgaccgtgt ggcggaacgg ctgccgctgg cgttgcacgg attgtctatg
     4081 tcgctgggcg ggcaagcacc gctggatact gatttgatag acggcatcaa agaaatgatg
     4141 cgccgttacg attgcacgtt tttctccgac catttgagct actgccacga cggcggtcat
     4201 ctttacgatt tgttgccgct gccctttacc gaggaaatgg tgcatcatac ggcgcggcgt
     4261 atccgcgaag tgcaagaccg tttgggctgc cgcatcgccg tggaaaacac gtcctactat
     4321 ctgcattccc cgcttgccga gatgaacgag gtcgagttcc tcaacgccgt cgcacgtgag
     4381 gccgattgcg gcattcatct ggatgtgaac aatatctacg tcaacgccgt caatcacggc
     4441 ctgttgtcgc cggaggcttt tttgaaaaac gttgacgcag atcgcgtgtg ctatatccat
     4501 attgccggac atgacgtgga aacgccggaa ttgttgattg atacacatgg cgcggcagtt
     4561 ttgccgactg tttgggactt gctcgaactt gcctatgcca agctgccgac gattccgccc
     4621 accctgttgg aacgcgattt taatttcccg ccttttgccg aactcgaagc cgaagtcgcc
     4681 aaaatcgccg attatcaaac gcgtgccgga aaggaatacc gccgtgcagc ctgaaacctc
     4741 cgcccaatac cagcaccgtt tcgcccaagc catacgcggg ggcgaagccg cagacggtct
     4801 gccgcaagac cgactgaacg tctatatccg cctgatacgc aacaatatcc acagctttat
     4861 cgaccgctgc tataccgaaa cgcggcaata ctttgacagc gaagaatgga gccgtctgaa
     4921 agaaggtttc gtccgcgacg cgcacgccca aacgccctat tttcaagaaa tccccggcga
     4981 gttcctacaa tattgccaaa gcctgccgct tttagacggc attttggcac tgatggattt
     5041 tgaatatacc caattgctgg cagaagttgc tcaaattccg gatattcccg acattcatta
     5101 ttcaaatgac agcaaataca caccttcccc tgcggccttt atccggcaat atcgatatga
     5161 tgttaccgat gatttgcatg aagcggaaac agccttgtta atatggcgaa acgccgaaga
     5221 tgatgtgatg taccaaacat tggacggctt cgatatgatg ctgctagaaa taatgggttc
     5281 ctccgcgctt tcgtttgaca ccctcgccca aacccttgtc gaatttatgc ctgaggacga
     5341 taattggaaa aatattttgc ttgggaaatg gtcaggctgg actgaacaaa ggattatcat
     5401 cccctccttg tccgccatat ccgaaaatat ggaaggcaat tccccgggcc aaaaccatct
     5461 atccgcataa aattaccttg ttcccgatac tatgccgcta cccgacctga ccgatgccga
     5521 attaatagag tcgcgtaaac tgcttctgca ttttgcgcgg cttcagttgc ccgaccaccc
     5581 tgatttggct gaagatttag tgcaggaaac attgctgtcc gcatacagcg caggcgacag
     5641 ttttcaaggc agggcacttg tcaacagctg gctttttgcc atattgaaaa acaaaattat
     5701 tgacgcatta cgtcaaatcg gaaggcagag gaaagtcttt accacactgg atgacgagct
     5761 actggatgaa gcatttgaaa gccatttttc ccaaaacggg cattggacgc cggaagggca
     5821 gccgcaacat tggaacactc cggaaaaatc attaaacaac aacgaattcc aaaaaattct
     5881 gcaaagctgc ctatacaacc tgcctgaaaa caccgcacgg gtatttaccc tgaaggaaat
     5941 actcggtttt tcatccgacg aaatacaaca aatgtgcggt atcagcacgt ccaactacca
     6001 caccattatg caccgcgccc gagaatcatt gcgccaatgc ctgcaaatca aatggttcaa
     6061 ccaagaaaac ccgaagtaaa cgttatgaaa aaatgccgcg atatcgccct gcttctttcc
     6121 aaacatcagg accgggaaac caccccgggc gagaagattt ccatatacac acacctgctg
     6181 ttctgtccgt attgccgtga atataaaaga caacttcaaa ccatcaaaag atcactggca
     6241 aaaacaacca gaacttcaaa ataaatgccg tctgaaaagg cttcagacgg cataagctga
     6301 cggaaacaaa tcaaaccgat ttactgttat cggcagttca tccataatac acgcttcaaa
     6361 agcagcatat tcctccatac ggaatatata aatacgcaaa acacgaaggc tgcatcaatt
     6421 tgctttattt gccttatttc acagacggcg ctacccctcc cgcccaaccc gttctttctg
     6481 aatgagcaga tttcaatgat taaggaaacc ctaatgcgcc caatcttcct atctttcgtt
     6541 ttactcccta ttttgataac cgcctgcagc acaccggaca agtctgcccg atgggaaaat
     6601 atcggcacaa tctcaaacgg caatattcat acatatatca ataaagacag cgtgagaaaa
     6661 aacggaaatc tgatgatttt ccaagataaa aaagttgtta ccaatctaaa acaagaacgt
     6721 tttgccaaca cccccgcata caagactgcc attgccgagt gggaaatcca ctgcaacaac
     6781 aaaacatacc gcttaagttc gctacagttg tttgatacaa aaaacacgga aatttccaca
     6841 caaaactaca cagcctcttc cctccgcccg atgagcatcc tgtccgggac attaaccgaa
     6901 aaacaatatg aaaccgtatg cggaaaaaaa ctctgattgc aacttataca caaacttacc
     6961 cacaaacctt atcataaaaa tgccgtatga aatactgaaa tatcagcatt tcagacggca
     7021 ttttgccatt ccctgaaaat tatccacaaa gttatccaca ttatttttta aaaccggctt
     7081 ccatccga
//
LOCUS       DAWFAD010000103         7007 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWFAD010000103 DAWFAD010000000
VERSION     DAWFAD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.103"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(75..1496)
                     /locus_tag="WGO49_001528"
     CDS             complement(75..1496)
                     /locus_tag="WGO49_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ0306624.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINTMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(1854..2213)
                     /locus_tag="WGO49_001529"
     CDS             complement(1854..2213)
                     /locus_tag="WGO49_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ0306625.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(2210..2815)
                     /locus_tag="WGO49_001530"
     CDS             complement(2210..2815)
                     /locus_tag="WGO49_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ0306626.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVIENGLEGIVQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(2862..3344)
                     /locus_tag="WGO49_001531"
     CDS             complement(2862..3344)
                     /locus_tag="WGO49_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0306627.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      3548..3645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3856..4956
                     /gene="gcvT"
                     /locus_tag="WGO49_001532"
     CDS             3856..4956
                     /gene="gcvT"
                     /locus_tag="WGO49_001532"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ0306628.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVHNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEADVRVLKLPFVRNGQKQFD"
     gene            5119..5505
                     /gene="gcvH"
                     /locus_tag="WGO49_001533"
     CDS             5119..5505
                     /gene="gcvH"
                     /locus_tag="WGO49_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ0306629.1"
                     /translation="MSSNIPAELKYVASHEWLRLEEDGIITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDPYGAGW
                     FFKLKPANVADYDVLLTAEQYAGEVD"
     gene            5657..6904
                     /locus_tag="WGO49_001534"
     CDS             5657..6904
                     /locus_tag="WGO49_001534"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ0306630.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAACLPEAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEISPYLYTLGMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSIQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         1783 a         1677 c         1974 g         1573 t
ORIGIN      
        1 tgtcggaagg ggggatattg atttgattat gccggaattt aaaatgccgt ctgaatgttc
       61 agacggcata gcgtttacag cagtttgaaa acgaaaaaga taagggtatg tacgatgaag
      121 acgggtgtca ggaaggcgac cgaccacatc atatagccga agaaagtcgg catcggtacg
      181 ccgcgctgtt cggcaatggc cttgaccatg aagttcggtg cgttgccgat gtaggtcagt
      241 gcgcccatga ataccgaacc catagaaacc gccagcagcg aatgaaacag ggtacccgtc
      301 atcaaggctt gggcatcgcc gcccgccata ttgaaaaaaa cgagataagt gggcgcgtta
      361 tccaagaatg ccgacaatat gccgctcatc caaaaataca tcgtattaat cggatgacct
      421 gccgtatcgt gaaccagcga taccaccccg cccagcgcgc ctgcctcgcc tgctttcaga
      481 atgctcagga cgggaaagat ggtgatgaag atgccgagga agagtttgcc cacttcggcg
      541 atgggttcaa agttgaattc gttgcctgcg cggacttgtt tgggcgtgat tgccatagat
      601 acggcggtca atgcaatcag gatgacatcg cggacgaggt tttgcagggc gtaacggctg
      661 ccgaggattt caaatcccgg gtgttcgggt ttccaaaggc cggacattag aaccgcgccg
      721 accacgcccg aaagcaggag gaagttccat ttgccgaaga tggcgatttt ttcgggtttt
      781 tcctgttgtg ccggcgtatc ttgtgcaatg ctttcctgtt tgaagaaacg gttgtcgatg
      841 aaatagaagg cggtcaacag gacagcggtg ctgatcagga cgggggcgaa catatgtttg
      901 accgtccaca tgaaatctac gcctttgagg aagccgagga agagtggggg gtcgcccaaa
      961 ggggtcagac cgccgccgat gtttgcaacc aggaaaatga agaagatgac gatgtgcacg
     1021 cggcgggtac ggttttggtt ggctttcagc agcggacgaa tcatcagcat tgctgcgccg
     1081 gtcgttccca tgatagaggc aagtgccgta ccgacggcaa gcagggcggt gttgagcttg
     1141 ggtgtgccgt tcaagtcgcc ccaaaccaaa atgccgcctg aaatggtgta cagggcaagc
     1201 agcagcagga tgaaggggat gtattcttca acgagtgcgt gtgcgacggt atggataccg
     1261 gcggacgcgc caaaaaccaa actgaacggg atgaggaaga gcaatgtcca aaaggcggtg
     1321 attttgccgt aatggtgatg ccaggtatgc gaaaaaaaca agggacccaa tgcgatagac
     1381 agcaaaatca gggcaaaggg caggctccac agcaggttta ggtttgcgcc gtccaaatct
     1441 gcggcgtaaa ccgatgcggg gaaaagcatt agtgaaaaca ggggtaggtg gcgcatcgtg
     1501 tttcctcgat tcaagcactg ccttgcgcgg cgcgtgggag tgatacaggc accgtgccgc
     1561 ccggacatag gcggactgtg ctgcctgttt gtttttgaaa aaagttttcc gattgattgt
     1621 aaagtaaata atcaggataa accagtttcc caaaccggaa ggcggcggga aggcggattg
     1681 ctgtgcttgg gaatatatgt tctttttgat aaataatttt atttaaaaca aataaatata
     1741 tgtctttaat aaataatcta tcgaaaacga aaaatgaatt tattttaaca tatatttgca
     1801 atgaaacagg tttgcccccc gtttgtttgc ccttatccct ttcagtacgg cattcaagat
     1861 tcgggcctgc gccacatcca tatggcgaca agggaacaaa aaaccgatga aaccgccccg
     1921 acccaccagc gttggggaaa ctgccaaaac attatcaggc aggatgcggt catcatactg
     1981 atggcgaata ttttggcttt gcgcggcact gcgccgtttt gttcccagtt atgaaccatc
     2041 ggaccgaaat agcggtgccg gtgcagccag cggtgaaagc gcggggatgc ctttgcccag
     2101 caggcggcgg agagcagtac gaacggcgtg gtcggcaaca gcggcaaaaa aatgccgatg
     2161 atacccaaca gcagggagat gcagccgcag gcaattaaaa gataacgtat cattttgaaa
     2221 tatttttctt attgtgcgga taagggcagg atgtgatacc gagttttgcc cagccttcat
     2281 gtcccatttt ttccagcagg gcgatattgc gttcgaatat tgccgaagcg tcgggaaagg
     2341 cttgtgcggc tttggcaatg ctgtcttcgc ggatgaggtg cagcgtcgga tagggagaac
     2401 ggttggtgta gttgccgatg tcgtcgggtt cggtattctg aaagcggaat tggggatgga
     2461 acggtgcgat ttggacgatg ccttccaagc cgttttcgat aacggcggca tcggcaatat
     2521 cgagcatatc gttgaatacg tcgaaatcgg ggaatagggt cgggtgaacc agcagggtgg
     2581 tttccagttc ggtggcgggt gtattgccca gtcgctgcag ttcttcgtcc aagtcttcca
     2641 aaaaactgtc aaggtgtttg gcttcgctga ttgcgatgcg gacaaggttt ttaacgtggg
     2701 gggctttggc aaagggacac aggttcagac cgatgacggc tttttccaac cattgtccgg
     2761 tgtgttcggc aacagcatct ttattttcgg aagtattgat attcattatt gtcatgtaaa
     2821 tgtgtttgca gattgcacgt gcgggaaaat cgggaagggc actattcctt cagcaggtgg
     2881 ttgagcggca gggaggtggt gtgtttgatt tcttttaaaa caaagctcga ttgcgcatct
     2941 tgtacgccgt ggtgggacag gagcgtatcc aaaacaaaat gggaaaacgc gttcatatcg
     3001 gtaaaaaacg cctgaagcag gtagtcggtt tcccctgtca gggcgaagca gctcaagact
     3061 tcaggccatt ttcgaaccga tgcggcaaag tcttcccgcg cgtcttttgc tttgcggatg
     3121 gaaacgcgga taaatgcctg aagccccagg ttgacggatt ccggagacag cagcgcggca
     3181 tattggcgga cgatgccggc atcttccaac tgcttcagac ggcgcaggca cggagaaggc
     3241 gaaagtgcga cacgttcgga cagttcgaca ttggtcagcc tgccgttttc ctggagaacc
     3301 tgtaagattt taatatcggt tttgtctaaa gtgagttggg gcatatttgc gttccgtttt
     3361 aaggaattcg gattgtctgt ccgtatgttt gcggcaatcc gcacagatgg agaccatatt
     3421 aacatataaa aagttatacc gtcatccggg acaaattttg ttttcggaaa atcatgtgaa
     3481 aacagaggcg gtcggtttgc atctctttta agacggcttg cccaaaccgc cgattcaaga
     3541 cataatcggg aaatgtgcag gagagtgtta cacccaacta caatgtaacc accgaaggcg
     3601 cagacaccct taaatcgctc aggtatcagg gactgcacat tgaaacaaac aatctggaga
     3661 gcggcgttgg aataacgtcc accgaagggg agaaggccgt ctgaaccacc attcagacaa
     3721 ccgcgcaaag cagtgagcag actggtttgc catcatgcgg atacggccga aaatctcagg
     3781 ttcaaggaca gatagggtca tccgcgcaca ggtgcgcggg tggcatctga acaaaaaatc
     3841 cggagaaact tgagaatgat tactctgaaa accaccccat ttcatcaagc ccatcaagat
     3901 gcaggcgcga agccggtaga ttttgccggc tgggagctgc ccatccatta tggttcgcaa
     3961 atcgccgaac acgaagccgt gcgcaccgac gccggtatgt ttgacgtatc ccatatgctt
     4021 gttaccgacg tagcaggcgc aaatgccaaa gcctttttcc gcaaattgat tgccaacgat
     4081 gtcgccaagc tcgcttttgt cggcaaagcc ctttattccg ctttgctcaa cgacaacggc
     4141 ggcgtgattg acgacttgat cgtttaccgc accaatgaag ccgaaaccca ataccgcatc
     4201 gtgtccaacg gcgcgactcg cgaaaaagat acggcacaat tccacaaagt cggacaagag
     4261 ttcggtgttt ccttcaatcc gcgctacgac ctcggcatgc tcgccgtaca aggccctaaa
     4321 gccattgaaa aactcctgac cgtcaaaccc gaatgggcag atgtcgtcca taacctcaaa
     4381 ccgttccaag gcgcggattt gggcaacgac tggtttgtcg cccgcaccgg ctacaccggc
     4441 gaagacggcg tcgaagtcat cctgcccggc accgaagccg tcgcattctt caaagccctg
     4501 caagcagcag gcgtacaacc ttgcggtctc ggcgcgcgcg acaccctgcg catggaagcc
     4561 ggcatgaacc tctacggcaa cgatatggac gacgatacca gcccgctcga agcaggcatg
     4621 ggttggaccg ttgatttgaa agacgaaagc cgcgattttg tcggcaaagc tgccttgttg
     4681 gcattgaaag aaaaaggcgt tgccgtcaaa caggtcggtt tgctgctcgg caaaggcggc
     4741 atcctgcgcg cgcatatgga agtgttgacc gacaaaggca aaggcgaaac caccagcggc
     4801 gtattctctc caagtctgaa acaatccatc gccatcgccc gcgtaccgaa agattttgac
     4861 ggcgataccg ccaaagtgct gattcgcggc aaggaagcgg acgtgcgcgt actgaagctg
     4921 ccgttcgtcc gcaacggtca aaaacagttt gattaatatc ggttcagacg gcattttcat
     4981 ttcatatgcc gtctgaaagc aggttttaat tgtcgtccga tacggacgtt tgtagaaagc
     5041 attgaacaag gcatctgtgg atattgattc atgcagatgc cgtctgaaaa taacccccta
     5101 tcaatggagt atcaaaccat gagcagcaac atcccggcag aactgaaata cgttgccagc
     5161 cacgaatggc tgcgccttga agaagacggt atcatcaccg tcggtattac ccaccacgcg
     5221 caagagctgt tgggcgacat cgtgtttgtc gagctgcctg aagtcggcgc aaaccttgcc
     5281 gccgaagaac aagccggtgt ggttgagtct gtaaaagccg cgtccgacgt gtacgcaccg
     5341 attgcaggtg aagtcgttgc cgtcaacgaa gatttgccaa gcgctccgga aactgccaac
     5401 agcgatcctt acggtgcagg ctggttcttc aaactcaaac cggcaaacgt tgccgattac
     5461 gacgttctgc tgactgccga acaatacgcg ggcgaagtgg attaaaccgc ccggctgccc
     5521 gatggcaacc gccggacaaa cggaaactgc accttcagac ggcatttttg cggtcggagg
     5581 tgcagttttt tgtccgtgtt ttaaggaagc agttaggcta taataacggt ctatattcat
     5641 ctttaccgat tttttcatgc aacttaccgc tgtcggactc aatcatcaaa ccgcaccttt
     5701 aagcatacgg gaaaagctgg cgtttgccgc ggcctgcctg cccgaagccg tccgcaatct
     5761 tgcccgaagc aatgcggcaa cggaggcggt aatcctttct acctgcaacc gtaccgagct
     5821 ttactgtgta ggtgattcgg aagaaatcat ccgttggctc gcagactatc acagccttcc
     5881 catagaagaa atcagcccct acctttatac tttggggatg caggagactg tgcgccatgc
     5941 tttccgcgtc gcctgcggct tggattcgat ggtgttgggc gagccgcaga ttttaggaca
     6001 gattaaggat gcggtcaggg ttgctcaaga gcaggaaagt atgggtaaga aactcaatgc
     6061 cctgttccaa aaaacctttt ctgttgctaa agaggtccgt accgatactg ccgtcggcga
     6121 aaactcggtt tccatggctt ccgcttccgt caagttggca gagcagattt tccccgacat
     6181 cggcgatttg aatgtcttgt ttatcggtgc gggtgagatg attgagctgg ttgccactta
     6241 ttttgccgcc aaaagtcccc ggctgatgac ggttgccaac cggacgctgg cgcgtgcaca
     6301 ggagttgtgc gacaagctcg gtgtcaacgc cgaaccgtgc ctgctgtccg atctgcctgc
     6361 cattttgcat gagtacgacg tggtggtttc ttcaacggca agccagttgc ccattgtcgg
     6421 caaaggtatg gtggagcgcg cattgaaaca aaggcagagt atgccgttgt ttatgcttga
     6481 cttggccgtg ccgcgagaca ttgaggcgga agtcggagat ttgaacgatg cctatcttta
     6541 tacggtggac gatatggtca atatcgtcca aagcggcaag gaggcaaggc agaaggccgc
     6601 cgccgccgcc gaaacgctgg tgtccgagaa ggttgccgaa tttgtcaggc agcagcaggg
     6661 caggcagagt gtcccgttaa tcagggcatt gagggatgag ggagagaaag cgcgcaaaca
     6721 ggtcttggaa aatgcgatga aacagcttgc caaaggcgca acggcagaag aggttttgga
     6781 aaggctgtcg atccaactga ccaacaagct gctgcattcg ccgacccaaa ccttgaataa
     6841 ggcgggggaa gaagataaag atttggttca cgccgtcgcg cagatttatc atttggacaa
     6901 ataacggtgc gccggaaaag gatgccgtct gaaggggttt cagacggcat tttttgtgca
     6961 ggccggcaac atcgtgaaat cccacattat atcgatgtaa tcacaaa
//
LOCUS       DAWFAD010000104         6970 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWFAD010000104 DAWFAD010000000
VERSION     DAWFAD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.104"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            337..636
                     /locus_tag="WGO49_001535"
     CDS             337..636
                     /locus_tag="WGO49_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0306631.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPAGMAVSSLPPQPH"
     gene            576..812
                     /locus_tag="WGO49_001536"
     CDS             576..812
                     /locus_tag="WGO49_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0306632.1"
                     /translation="MRLRDAGGYGGFFPAAAAALTQRPYATLDLTAHYKIGKSTRIGL
                     DFENVFNKRYRPMPDIHVYGTPRSLTATVKHIVD"
     gene            complement(1004..2407)
                     /gene="mtrE"
                     /locus_tag="WGO49_001537"
     CDS             complement(1004..2407)
                     /gene="mtrE"
                     /locus_tag="WGO49_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ0306633.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(2462..5665)
                     /gene="mtrD"
                     /locus_tag="WGO49_001538"
     CDS             complement(2462..5665)
                     /gene="mtrD"
                     /locus_tag="WGO49_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ0306634.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYLSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(5677..6915)
                     /gene="mtrC"
                     /locus_tag="WGO49_001539"
     CDS             complement(5677..6915)
                     /gene="mtrC"
                     /locus_tag="WGO49_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ0306635.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFIVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWAPSENQA
                     AAPQSGVQTASEAKTASEAE"
BASE COUNT         1616 a         1953 c         1827 g         1574 t
ORIGIN      
        1 atcaaacatc aaattggact gaccaatcag ggcggattct aatgacacgc cgttcccgcc
       61 gtcaacggca tttacctcgc accgcccccg aaacacaaga aaaaactaca ccaaactaca
      121 atttttgttt atacaaatat ttgttttgac aggatttaaa cgaaagctcc gattcaaatc
      181 tgccgaaccg cccaaaaata tatagtggat taacaaaaat caggacaagg cgacgaagcc
      241 gcagacagta cagatagtac ggtaaggcga ggcaacgccg tactggttta aatttaatcc
      301 attctattga cctgaatatt aaagtttcgc aaagtaatgc aacgttgctt taattggttt
      361 gaccactatt gccgacgatt agaaaaatat tttcggagat gttcaattat ggaaacttgg
      421 gttcaaaact acacggcaat cggcggcagc ctgtatctga ctgccgccgc cgcactctta
      481 cccatcgtct ttttctttgc cgcgctgacc gtcctgaagc tgaaaggcta tcaggcgggg
      541 ctttatacgc tgctgattgc gcttgccgtt gccgtattcg gcttcgggat gccggcgggt
      601 atggcggttt cttccctgcc gccgcagccg cactgaccca acgcccctac gccacgctgg
      661 atttgaccgc gcattacaaa atcggcaaat ccacccgcat cggtttggac tttgaaaacg
      721 tgttcaacaa acgctaccgc cctatgcccg acattcacgt ttacggcacg ccgcgcagcc
      781 tgaccgcaac cgtcaaacat atagtggatt aacaaaaatc aggacaaggc gacgaagccg
      841 cagacagtac aaatagtaca acttggtgct tcagcacctt agagaatcgt tctctttgag
      901 ctaaggcgag ccaacgctgt actggttttt gttaatccac tataaagaaa gaaatgccgt
      961 ctgaaacctt atcgtttcag acggcatttt attgcccgac cggttatttg tcggtttggg
     1021 tatcccgttt caatccgccg ccgagtgcct tgtacaaatc ggcaaggttt tcggcgcggg
     1081 tcagttgtgc cgacaaagcc gcaccttccg ccgaatagct gctgcgttcc gcatcgagca
     1141 agtcgagcgc gccggatacg ccgtgtttgt aacgcagacc gaccaaacgc aacgcttctt
     1201 tagaggcgcg gctttgtttg cttaaagcgt cataggcttt atccaactgc tcgcgcgccg
     1261 ccaatgcgtt tgccacgtct tgaaatgcgg attggacggc ggattcatag gcaacgattt
     1321 gtgcctgttg gcgcagcttg gctacatcaa ggttcgcctt gttcgtaccc caggtaaaaa
     1381 tcggcagggt aatagacggc gcgaacgacc aaacgcccgt gccgcttttg aacaacccac
     1441 ccaattcggc agaacccgta ccgacggttc cggtcaggcg gatggatggg aaaaaggcgg
     1501 cgcgtgccgc accgatattg gcgtttgcct gtttgagcgc gtgttcggca gcacggatat
     1561 cgggacggtc gagcaatact tcggaactca aaccggccgg cagtttttca acaaaaaact
     1621 gcttgtccag cggcaaaccg gcaggcaggt cttcgggtat cggttggtta atcaaggttg
     1681 ccaaggcatt gcgcgcctgt tcgcggctgc gcgcggcatg ggcataatcg gctttggcag
     1741 attcgattag ggcttcctgc tgacgtaggg cgacggcgga aatcacgcct gccttgtaac
     1801 gtaattcgga cagcttgtag gtttcctcgc gcgttttcaa aacacgttgc gccaaagaca
     1861 tcgcttcttc ggcgtaacgt tcgttgaaat aggctttggc aacggtggca atcaggctca
     1921 aatgtgccgc atcgcggttg gcggtgctgg cgaaatagcc ttgcagtgcc gcctcgctgc
     1981 tgctgcgtac acgcccgaac aaatcgagtt cgtaagatgc cgcacccagt ccgactttgt
     2041 agctgctgct tacattgccg ccgctcaagc tgccttggcg cgagtcgttc gcattggcgg
     2101 caagcgtggg caggaggttg ttgcgctcaa tcatgtattg tttgcggtag atttcgctgt
     2161 tcaatacggc ggtacgcaaa ctggtattgc gctcgagtgc gatgtcgatc agcttttgca
     2221 ggcgcgggtc ggcaaaatag tcatgccaac ctaaatcgac cgcacggatg ccgctgtcgg
     2281 cggtatcgtt tttgaacgtt tcggcaactt cgactttggg ctgctcgtat tggggaatca
     2341 tggtgcaggc ggataatgcg aaggctgctg caacagaagt caaggtggtt ttcaatgtag
     2401 tatccataaa aaagtcctga tgccgtctga aaacccgtag gcgttcagac gtcatggttg
     2461 cttaatgttg gctgtcgtcc gaaccggtga tgcccgcttc ggcggcgtgt tttactgcca
     2521 tttcgtgttc gtgcgcggtt tctttgaaga atttgcgcac caccacatag aaaagcggaa
     2581 caaggaacac ggacaagagc gtgccgatga gcatccccca gaatacggtc gtgccgatgg
     2641 cgcgctggct ggcagaactt gcaccgccgg caatatacag gggaaccacg cccaaaataa
     2701 aggcgaacga ggtcatgata atcggacgga aacgcaggcg ggcggcttcc aaagcagctt
     2761 cgaccgcgct tttcccttgc gcttgaaggt ctttggcaaa ttcgataatc aaaatcgcgt
     2821 ttttcgcact caaacccatc acggtaacga aaccgacttg aaagtagatg tcgttggcga
     2881 acgagggaac gctgcccaac agtccttcaa acaggttgcg cccggttacg cccgcagccg
     2941 caccgatcaa acccaacgga atcacaagga tgaccgccag cggaatcgac cagctttcat
     3001 aaagcgcggc aagtaccaaa aatacggctg caaccgccaa accgtacaaa atcagggttt
     3061 gcgagccgcc ttttgcctct tcgcgcgact gtccgcccca ttcgaagctg taaccgccgc
     3121 ccaattcgtc aaccattttt tgaaccgccg ccatagcctg cccggtggaa acgccggttg
     3181 caggcgaagc ggacagcttc atcgaaggat aaccgttgaa gcgtacgctc tgttccgtac
     3241 cgttttccca agaaacagta gcaatggtgg aaagcggtac ggcgacgccg gatttgttcg
     3301 gcacggtcag gttcaaaata tcggcaggct gcatacgggc atcctcgtcg gcctgcacca
     3361 tcacgcgttg cagacggcct tggttcggga agtcgctgac ataagacgaa ctcagcgcac
     3421 ttgccaatgc ggtgcggatg tcggcaaacg aaatgccttg cgccgccgcg gcggcacggt
     3481 tgatgtcgat tttcaactgc ggcgagtctt ccaaaccgcc ggcacggacg gtgctggggt
     3541 caaacaaacc gctggcacgc attttctgaa tcaactcgtt gcgcttcgcc agcaatgcgg
     3601 tatggccggt attgttgcgg tcttgcaggt tgatgctcag gcccgaacca ttgcccaact
     3661 ccaaaatcgg aggcgggacg acggcgatgc caaaaccgtc tttaagcgtc cccatcatca
     3721 tacccgtcag cttgccggca atcgcaacgg catcgctgcc gggcgcggta cgctcgttcc
     3781 aatctttcaa tatggcaaaa cccatcgcca tattctgacc gctgcccgaa aagctgaagc
     3841 cggaaacggt aatgatgttt tctatttcag gaatgctttt cgccagttgg gtaacttgcg
     3901 ccaaagtcgc attggtgcgc tcttgggtcg ctcctgcagg cagttgtacg ctgaccatga
     3961 cgaagccttg gtcttcggtc ggcaggaacg aagtcggcag gcgcataaac aggaacacgc
     4021 ccacaaccgc caagccgata tagacaacca tcatgcggaa agtcttacgc agcactttgg
     4081 caacccggcc ttcgtaaccg tgcgtccaac tgttgaattt cttgttaaac cagccgaaga
     4141 aacctttttt ctcttcgtga tgccctttcg ggattgtctt caacattgtg gcacacaaag
     4201 caggggtaag ggtcagcgca aggaaggcgg aaaatgcgat tgatgacgcc atcgtcaggg
     4261 caaactgttt gtaaatattg cccgtcgccc cgctgaacat cgccaacggt acgaacacgg
     4321 aaatcagaac ggcggtaata ccgatgaccg cgcccgaaat ctgacccatc gcttttttgg
     4381 tcgcttcttt gggcggcaag ccttcacccg ccataatgcg ctcgacgttt tcaaccacca
     4441 caatcgcgtc atcgaccacg atgccgatga ccaaaaccat cgcaaacatg gtcagtacgt
     4501 taatcgacat gcccatataa gagatgaagg cgaaaccgcc caacagcgaa atcggtacga
     4561 cgatggtcgg aatcagcgta taacggatgt tttgcaggaa gagatacatt acgacaaaca
     4621 ccagcaccat cgcttcgatt aaagtatgaa tcactttttc aatcgaaatt tcgacgaatt
     4681 tggaagtatc gtaaggggtt ttccagctca taccctgagg aaagtatttt tccaacgtcg
     4741 ccatgcgttc tttaaccgcc tttgccgtcg ccatcgcatt gccgctgttg gacagcatca
     4801 ccgccatacc ggtggtattt acaccgttca gacgggttga ggaagaatag tcttccatac
     4861 ccagtccgac ccttgccaca tccttcaggt aaacattaga accgtcggta ttggcgcgga
     4921 ggatgacgtt gccgaattct tctgccgtac ccaactgccc ttgcgccgtt acggtagccg
     4981 taaccgtctg tccgcgaacg gcgggaagcg aaccgataga acccgctgaa atctggacgt
     5041 tctgggcgga cagcgcactg ccgatatcgg caaacgacaa attgtagttt tgcagtttct
     5101 taggatcaac ccaaatccgc atcgcgcgtt gcgcgccgaa caggcgtacc tgccccacgc
     5161 cttcgatacg ctgcaactcg ggaacgatat tacgctgcgc gtagtcgttc atctcttcgg
     5221 ttgactgcac atccgacgaa agcatcacaa tcatcaggaa attggaacgc gccttggata
     5281 cggttacgcc gtattgctgg acggttgccg gcagcgtgct caatacttcg gaaagcttgt
     5341 tctgcacttc cacctgcgcc agattctcgt cggtatcggg cgtaaaggtc aggctcacgc
     5401 tgccgctgcc gctcgaatcg gcagaagtgg acagataatc caaaccttcc acgccgttca
     5461 tattccgctc gatcacggaa agcacgctgt cttccattac ctgcgcggac gcgcccggat
     5521 aagtggccct cagggtgatg gtcggggcgg cgacagacgg atattgcgaa acaggcaggc
     5581 ttttaatgcc gaaaataccc gccgcaataa tgaaaatcga aataacccac gcgaaaatgg
     5641 ggcggtcgat aaaaaattta gccatcgatg ccttccttat tccgcttcag aagcagtttt
     5701 ggcttcagat gccgtctgaa cgccggattg aggcgcggcg gcttggtttt cagacggcgc
     5761 ccattctttg ggcgttacct ttttcgcacc cgttataccg gcgatactga tgccttccac
     5821 aaccaccttg tccccgtcct tcagacccga cgtaacaatc caattcgtac cctgctgttg
     5881 cgcgaccgtt acctcgcggg gttccatacc gccttgggcg ttcacaatca tcacggtatc
     5941 tttcgcaccg cgcgttaccg cctgctgcgg cacgatgaat gcgttatcca ccgccacttg
     6001 gtccatcagc acgcgcacat acaggccggg catcaggata ttctgatcgt tcggtacggc
     6061 ggcgcgcagg gtaatctgac cggtcgattc gttgacggtc ggatcggcaa acagcaggcg
     6121 gcctttttca gggtaaactg tgccgtcgtc aaatttgatg ccgaccgcaa tcacaccatc
     6181 cgccgccagc agtttgcctt cggctatctg acggcgcaat ttcatcactt cggatgcaga
     6241 ctgggtaacg ttcacataca tcggattggt ttggcggatg gttgccagca cggtcgtatc
     6301 gcccgcattc aacagcgtac cttcggaaac tttggactga ccgataaagc cggaaatcgg
     6361 cgcggtaatg cgcgaacggt tcagattaat gccggcagat ttgattgccg cctgtgctgc
     6421 tttgacacct gcctcggcag aacgtttcgc cgttaccgca gcatcgtatt cctgccggct
     6481 gacggcttcg gcggcaacca aaggcttgta ccgcgccaaa tccgcatccg ctttggcaag
     6541 cgttgcctga gccgttgcca gttgcgcgcg tgcgctttcc agacctgctt cataagtgga
     6601 actgtcgatc tgatacagcg gctgtccggc acggacataa ctgccttctt ggaacaggcg
     6661 tttttggatg atgccgccga cttgggcgcg gacatcggcg gtacgcagcg attccaaacg
     6721 ccccggcaac tcgacggtca atgcgacggt ttgcggatgg acggttacga caccgacgac
     6781 gggagccggt gcttcccgac cagcaggctg cccgccctgc gccgcgtctc cgcctttacc
     6841 gcaagacgac agtaccaatg caacggcggc agccaacgcg gccgcacgca tcgccttaaa
     6901 agcataaaaa gccattattt atcctatctg tctggtttga tgtaaagggt tttgccaatc
     6961 aacaggcatt
//
LOCUS       DAWFAD010000105         6774 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWFAD010000105 DAWFAD010000000
VERSION     DAWFAD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.105"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            120..1025
                     /gene="glyQ"
                     /locus_tag="WGO49_001540"
     CDS             120..1025
                     /gene="glyQ"
                     /locus_tag="WGO49_001540"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0306636.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTLDGNTVTYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAVEGASLALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1102..1512
                     /locus_tag="WGO49_001541"
     CDS             1102..1512
                     /locus_tag="WGO49_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ0306637.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAA
                     DDAEAFGRFMDELIAYLEANDFEMAGGGDCGDFSCLFQKSAPRGLGETEKKAIISWLA
                     GREDVATLHATPLVDGFYGDDGWENYSETYKQAD"
     gene            1533..3596
                     /locus_tag="WGO49_001542"
     CDS             1533..3596
                     /locus_tag="WGO49_001542"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ0306638.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINAAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDVVNVSVLGLQSGNKTLGHRFLSNGEITIENADSYAAQMREQGKVVA
                     PFAERKAAIQTALERQARRLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTADPSHIIQGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3613..4659
                     /locus_tag="WGO49_001543"
     CDS             3613..4659
                     /locus_tag="WGO49_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0306639.1"
                     /translation="MPSEAFRRHRAYRENKLQSLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLEILIVDDGSTDGTLAIAKDFQKRDSRIKILAQAQNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTPPAGAWLDFAADGRMRRLFTL
                     RQYFGILRRLLKNR"
     gene            4701..5528
                     /locus_tag="WGO49_001544"
     CDS             4701..5528
                     /locus_tag="WGO49_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0306640.1"
                     /translation="MQNHVISLASAAERRAHIADTFGAHGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGMPVCQLDPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5540..5635
                     /locus_tag="WGO49_001545"
     CDS             5540..5635
                     /locus_tag="WGO49_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ0306641.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            5677..6519
                     /locus_tag="WGO49_001546"
     CDS             5677..6519
                     /locus_tag="WGO49_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0306642.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1744 a         1804 c         1779 g         1447 t
ORIGIN      
        1 aatttaatcc actatatcat gtatgccgtg ttttcaaatc agtctgtctt tgagataaac
       61 ctatccaaac agcaatggaa acagtaaaat aaacccttta ttttatcgaa tcataagcca
      121 tgcttacctt ccaacaaatc atcttcaaac tgcaaacctt ctgggcagac aaaggctgca
      181 ccgtcatcca acccttcgac atggaagtcg gtgccggcac atcccatccc gccacctgcc
      241 tgcgcgcgct cggtcccgag ccttggtttg ccgcctacgt ccaacccagc cgccgcccta
      301 aagacggccg ctacggcgac aatcccaacc gcctgcaaca ctactatcaa ttccaagtcg
      361 ccctcaaacc cgcccccgcc aatatccaag acctctatct tgattctctg cgcgaattgg
      421 gcatcgaccc caaagtccac gacatccgtt ttgtcgaaga cgactgggaa aatccgactt
      481 tgggcgcgtg gggtttgggc tgggaagtct ggctcaacgg catggaagta acccagttta
      541 cctacttcca acaagtcggc ggcattgact gcacccccgt actcggcgaa atcacctacg
      601 gcatcgaacg cttggcgatg tacctgcaag gcgtggaaaa cgtctatgac ctcgtttggg
      661 caaaaacgct ggacggcaat accgtaacct acggcgacgt gtaccaccaa aacgaagtcg
      721 aacaatccac ctacaacttc gaatacagcg atgccgattg gctgctgcgc cagttcaacg
      781 actacgaagc gcaagccaaa cgcctgcttg ccgttgaagg agcaagcctt gcgctgcctg
      841 cctacgagct ggttctcaaa gcggggcata cgttcaacct cttggacgcg cgcggcgcga
      901 tttccgtaac tgagcgggca acttatattg gacgtattcg tgcattgagc cgcgccgtgg
      961 cgcaaaaata tgttgaaagc cgcgaaaaac tgggcttccc tttaatgaag gcaaatgctg
     1021 cctaaggggg cgacgatttt ttacgttcgg gcggcacgat aaggcagccg tccggaagcc
     1081 attatatttt ttaggaaaat catgcctgtc tatttccgca ccccaagcag gaaacgcctg
     1141 cacaaaatga accgccgcca gcataaaaag ctgcatttgg gcgagtttca aacattgatt
     1201 ttcggcgttc aaggcagttt gcaccaagct gcggacgatg ccgaagcgtt tggccgcttt
     1261 atggacgagc tgattgcgta tcttgaggca aacgactttg aaatggcggg cgggggcgat
     1321 tgcggcgatt tttcgtgcct gtttcaaaaa tccgcccccc gaggcttggg cgaaaccgag
     1381 aaaaaagcca ttatctcttg gctggcaggc cgcgaagatg tcgccacttt gcacgccacc
     1441 ccgctggttg acggttttta cggggatgac ggctgggaaa attacagcga aacctataaa
     1501 caggcagatt aaccaaattc aaagaaagcc gaatgatgac ccaaaccctt ttaatcgaac
     1561 tccttaccga agaactcccg cccaaagctc tgaataatct gggcaaccat tttgccgccg
     1621 ccgttgccga aggcttggaa aaagcgcaac tggttgacgg tgcagccgaa tttacggctt
     1681 atgcctcgcc gcgccgtttg gcggttcaag tcaaaaacgt caaagccgtt caggccgacc
     1741 aaaaaatcgt gaaaaaaggc cctgccgtgg cgaatgccat gaaagacggt gcgccgacca
     1801 aggctttgga aggtttcgca cgcggtgcgg gcgcgaaaat cgaagacttg accatcgtcc
     1861 acgacggcaa gcaggacgtg tacgcctacg aatacgtcca aaccggcaaa ccgttgggcg
     1921 agcttttgga agacattatc aatgccgcag tgaaaaaatt gccgattcca aaagtaatgc
     1981 gttggggcag cagcacattt accttcgtgc gccccgtcca cgggctgatt gtgctgcacg
     2041 gcggcgatgt cgtgaacgtc agcgttttgg gtctgcaaag cggcaacaaa accttgggac
     2101 accgcttcct ctccaacggc gaaatcacca ttgaaaacgc cgacagctac gccgcacaaa
     2161 tgcgcgaaca aggcaaagtc gtcgctccgt ttgccgagcg caaagccgcg attcagacgg
     2221 cattggaaag gcaggcgcgc cgtctgaacg ccgccgtcgc cgccgatgaa gccttgttgg
     2281 acgaagtaac cgcgctggtc gaatggcctg tggtattgga agccggtttt gaggaacact
     2341 tcctcgccgt gcctcaagaa tgcttgattt tgacgatgca gcaaaaccaa aaatacttcc
     2401 cgctgctcga ccaaaacggc aagctgatga accgcttcct gctggtctcc aacctgcaaa
     2461 ccgcagaccc gtcgcacatc atccaaggca acgaacgcgt attgcgcgcg cgcctgtctg
     2521 atgccgagtt cttctacaag caagaccaaa aagcgactct ggaaagccgc ctgcccaagc
     2581 tggcaaacgt ggtgtaccac aacaaaatcg gttcgcaggc cgaacgcatc gaacgcctgc
     2641 aaagcattgc cgcccacatc gctaaagcgt tgggcgcaga tgctgccgca gccgaacgcg
     2701 ccgcacgttt ggcaaaagcc gacttggtga ccgaaatggt cggcgagttc cctgaactgc
     2761 aaggcacgat gggcaaatac tatgcccgct tggacggcga aaccgaagaa atcgccgaag
     2821 ccatcgagca gcactatcag ccgcgttttg ccggcgacaa gctgcccgaa agcaaaattg
     2881 ccgccgccgt ggcactggcg gacaagctgg aaaccctggt cggcatttgg ggcatcggtc
     2941 tgattccgac cggcgacaaa gacccctacg ccctgcgccg cgccgccttg ggtattttgc
     3001 ggatgctgat gcagtacggt ttggacgtga acgaactgat tcagacggca ttcgacagct
     3061 tccccaaagg tttgctcaac gaaaaaacgc cgtctgaaac cgccgacttt atgcaggcgc
     3121 gccttgccgt gttgctgcaa aacgattatc cgcaagacat cgttgccgcc gtactcgcca
     3181 aacagccgcg ccgtttggac gatttgaccg ccaaactgca ggccgttgcc gcgttcaaac
     3241 aactgcccga agccgccgcg ctcgccgccg ccaacaaacg cgtgcaaaac ctgctgaaaa
     3301 aagccgatgc cgagttgggc gcggttaacg aaagcctgtt gcaacaggac gaagaaaaag
     3361 ccctttttgc tgctgcgcaa ggcttgcagc cgaaaatcgc cgccgccgtc gccgaaggca
     3421 atttccaaac cgccttgtcc gaactggctt ccgtcaagcc gcaagtcgat gccttctttg
     3481 acggcgtgat ggtgatggct gaagatgccg ccgtgaaaca aaaccgcctg aacctgctga
     3541 accgcttggc agagcagatg aacgcggtag ccgacatcgc gcttttgggc gagtaaccgt
     3601 tgtacagtcc aaatgccgtc tgaagccttc agacggcatc gtgcctatcg ggagaataaa
     3661 ttgcagtctt tagtcagcgt attgatttgc gcctacaacg tagaaaaata tttcgcccaa
     3721 tcattagccg ccgtcgtgaa tcagacttgg cgcaacttgg agattttgat tgtcgatgac
     3781 ggctcgacag acggtacgct tgccattgcc aaggattttc aaaagcggga cagccgtatc
     3841 aaaatccttg cacaagctca aaattccggc ctgattccct ctttaaacat cgggctggac
     3901 gaattggcaa agtcaggaat gggggaatat attgcacgca ccgatgccga cgatattgcc
     3961 gcccccgact ggattgagaa aatcgtgggc gagatggaaa aagaccgcag catcatcgcg
     4021 atgggcgcgt ggctggaagt tttgtcggaa gaaaaggacg gcaaccggct ggcgcggcat
     4081 cacaggcacg gcaaaatttg gaaaaagccg acccgacacg aagatattgc cgactttttc
     4141 cctttcggca accccataca caacaacacg atgattatga ggcgcagcgt cattgacggc
     4201 ggtttgcgtt acaacaccga gcgggattgg gcggaagatt accaattttg gtacgatgtc
     4261 agcaaattgg gcaggctggc ttattatccc gaagccttgg tcaaataccg ccttcacgcc
     4321 aatcaggttt catccaaata cagcatccgc caacacgaaa tcgcgcaagg catccaaaaa
     4381 accgccagaa acgatttttt gcagtctatg ggttttaaaa cccggttcga cagccttgaa
     4441 taccgccaaa taaaagcagt agcgtatgaa ttgctggaga aacatttgcc ggaagaagat
     4501 tttgaacgcg cccgccggtt tttgtaccaa tgcttcaaac ggacggacac gccgcccgcc
     4561 ggcgcgtggc tggattttgc ggcagacggc aggatgcggc ggctgtttac cttgaggcaa
     4621 tacttcggca ttttgcgccg attgctgaaa aaccgttgaa aaacgccgtt ttatccaaca
     4681 gacaaaaaac aggataaatt atgcaaaacc acgttatcag cttggcttcc gccgcagaac
     4741 gcagggcgca cattgccgat accttcggcg cgcacggcat cccgtttcag tttttcgacg
     4801 cactgatgcc gtctgaaagg ctggaacagg caatggcgga actcgtaccc ggcttgtcgg
     4861 cgcatcctta tttgagcgga gtggaaaaag cctgctttat gagccacgcc gtattgtgga
     4921 aacaggcatt ggacgaaggt ctgccgtata tcgccgtatt tgaagatgat gtcttgctcg
     4981 gcgaaggcgc ggagaaattc cttgccgaag acgcttggct gcaagaacgc tttgaccccg
     5041 attccgcctt tatcgtccgc ttggaaacga tgtttatgca cgtcctgacc tcgccctccg
     5101 gcgtggcgga ctactgcggg cgcgccttcc cgctgttgga aagcgaacac tgggggacgg
     5161 cgggctatat catttcccga aaagcgatgc ggtttttcct ggacaggttt gccgccctgc
     5221 cgcccgaagg gctgcacccc gtcgatctga tgatgttcag cgattttttc gacagggaag
     5281 gaatgccggt ttgccagctc gatcccgcct tgtgcgccca agagctgcat tatgccaagt
     5341 ttcacgacca aaacagcgca ttgggcagcc tgatcgaaca cgaccgcctc ctgaaccgca
     5401 aacagcaatg gcgcgattcc cccgccaaca cattcaaacg ccgcctgatc cgcgccttga
     5461 ccaaaatcag cagggaaagg gaaaaacgcc ggcaaaggcg cgaacagttc attgtgcctt
     5521 tccaataaaa ggaggagaga tggacatcgt atttgcagca gacggcagga tgcggcggct
     5581 gtttaccttg aggcaatact tcggcatttt gcgccgattg ctgaaaaacc gttgaaaaac
     5641 gccgctttat ccaacagaca aaaaacagga taaattatgc aaaaccatgt tatcagctta
     5701 gcttccgccg cagaacgcag ggcgcacatt gccgatacct tcggcaggca cgacattccg
     5761 tttcagtttt tcgacgcact gatgccgtct gaagaactga atcggatgat ggcggaattg
     5821 gtgccgggat tggcaaaaca gcacctgttg agcgaagtgg aaaaagcctg ctttatgagc
     5881 cacgccgtat tgtggaaaca ggcattggat gaaggtctgc cctatgttgc cgtatttgag
     5941 gatgatgtcc tgcttggcaa agacgcagaa aagttccttg ccgaagatac ttggttggaa
     6001 gagcgttttg ataaggattc tgcctttatc gttcgtttgg aaacgatgtt tgcgaaagtt
     6061 attgtcagac cggataaagt cctgaattat gagaaccggt catttccttt gctggagagc
     6121 gaacattggg ggacggctgg ctatatcatt tcgcgtgagg cgatgcggtt tttcttggaa
     6181 aggtttgccg ttttgccggc agagtggatt aaagcggtag attggatgat gtttacttat
     6241 ttctttgata aggaggggat gcctgtttat caggttaatc ccgccttatg tacccaagaa
     6301 ttgcattatg ccaagttcct cagtaaaaac agtatgttgg gtagcgattt ggaaaaagat
     6361 agggaacaag aaagaagaca ccgccgttcg ttgaaggtga tgtttgactt gaagcgtgct
     6421 ttgggtaaat tcggtaggga aaagaagaaa agaatggagc gtcaaaggca ggcggagctt
     6481 gagaaagctt acggcaggcg ggtcatatcg ttcaaatagt ttgtgtaaaa tatagcggat
     6541 taaaattaga aatggataca ctgtcattcc cgcgcaggcg ggaatctagt ccgttgagtt
     6601 tcagtcattt ccgataaatc accgcaacgt taaatttcta gattcccgcc tgcgcgggaa
     6661 tggcggtggt gggatttctg tttttccgac aaattcctaa aacttaaagt tccgttattc
     6721 ccgcgaaaac agaaaaccaa aaatagaaac ctgaaaacag aaacctgaaa acag
//
LOCUS       DAWFAD010000106         6500 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWFAD010000106 DAWFAD010000000
VERSION     DAWFAD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.106"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            13..339
                     /locus_tag="WGO49_001547"
     CDS             13..339
                     /locus_tag="WGO49_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0306643.1"
                     /translation="MGEKIKDGKNQKTVLSREEEQKICNTFTHKQAVEDFSVVIGYDE
                     IKAKNHSLSAGQYFEVKIDYVDISADEFAQKMAGFSADLDKLFAESAELEKEIKDRLA
                     MLKFNS"
     gene            350..487
                     /locus_tag="WGO49_001548"
     CDS             350..487
                     /locus_tag="WGO49_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0306644.1"
                     /translation="MKPVKLKMPSESLSDGILFGLENTYAVLSLKERIQKTKATGIYR
                     K"
     gene            complement(647..1984)
                     /gene="ribB"
                     /locus_tag="WGO49_001549"
     CDS             complement(647..1984)
                     /gene="ribB"
                     /locus_tag="WGO49_001549"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ0306645.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPADIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPAAVICEIINDDGTMARMPELMKFAEEHNLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPAADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2206..3221)
                     /locus_tag="WGO49_001550"
                     /pseudo
     CDS             complement(2206..3221)
                     /locus_tag="WGO49_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(3389..3982)
                     /gene="ribA"
                     /locus_tag="WGO49_001551"
     CDS             complement(3389..3982)
                     /gene="ribA"
                     /locus_tag="WGO49_001551"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ0306646.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGNFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(3975..4652)
                     /locus_tag="WGO49_001552"
     CDS             complement(3975..4652)
                     /locus_tag="WGO49_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306647.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMDMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVQSAAVYALMPSENQAFQ
                     LICLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECADEAAQAEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            4806..4890
                     /locus_tag="WGO49_001553"
     tRNA            4806..4890
                     /locus_tag="WGO49_001553"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4840..4842,aa:Leu,seq:tag)
     gene            5411..6445
                     /gene="purM"
                     /locus_tag="WGO49_001554"
     CDS             5411..6445
                     /gene="purM"
                     /locus_tag="WGO49_001554"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ0306648.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGELDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQDLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT         1608 a         1593 c         1697 g         1602 t
ORIGIN      
        1 gacgcatcgg gcttgggcga aaaaatcaaa gacggcaaaa accaaaaaac cgtactttct
       61 cgcgaagaag aacaaaaaat ctgcaatact ttcacgcaca aacaagcagt ggaagatttc
      121 agcgtggtga tcggctacga tgaaatcaaa gcgaagaatc acagcttgtc ggcggggcag
      181 tatttcgagg taaagattga ttatgtggat atttccgccg acgaatttgc gcaaaaaatg
      241 gcgggatttt cagcggattt ggataaactt ttcgccgaat ctgccgaatt ggaaaaagag
      301 attaaggata gattggcgat gttgaaattc aattcataaa taccgatgaa tgaaaccggt
      361 caaacttaaa atgccgtctg aaagcctttc agacggcatt ttgtttggtt tggaaaatac
      421 ttatgccgtt ctttccctga aagagagaat ccaaaaaacc aaagccacag gaatttatcg
      481 gaaatgacaa aaacccgacg aaccggattc ccgcctgcgc gggaatgacg aattagaagt
      541 tacccgaaat ttgcaaaaac aaaatccaac ccaacagacc gggttttcgt ttgcacggaa
      601 ataatgcaat aaataaagca aatataaagt atttgaattt actatattat ttttccgctt
      661 cttcaaagcc gacgacttcc aaaccgaagc cggtcagacc ggtgaaagat gagggctgcc
      721 cgaggacgcg cagttttttg acgttgaggc cgacgaggat ttgtgcgccg atgccgtagc
      781 ttttgctgtc ccatttgtag gcttggtttg cgcctttggg tagggtgcgg tcgagcaggg
      841 atgcgccgtc ttcggtgcgg tgcagaagga tgacgacgcc gctttctgcc tgttggacgc
      901 gctcgagtgc tttgggcaat gaccatgaat ggcgcgggtt ggcttgaatg aagtccatca
      961 cactgaaggg ttcgtggacg cggacgaggg tttcggtgtc ggcggcgggc gtgcctttga
     1021 cgagggcgag gtgggtttcg ccggagagtt tgtcgacgta aacgtgttgt tggaactcgc
     1081 cccacggggt ttgtacaggc gcattgccca tgtcttcgag caggctttcg gtacggctgc
     1141 ggtattcgat gaggtcggca atggtgccga ttttaaggtt gtgttcttcg gcaaacttca
     1201 tcagctcggg catacgcgcc atggtgccgt cgtcgttgat gatttcacag atgacggcgg
     1261 caggaatcag cccgttcatt tgcgccaagt cgacgccggc ttcggtgtgt ccggcgcgga
     1321 cgagtacgcc gcctttttgg gcgcgaagcg ggaagatatg accgggttgg acgatgtctg
     1381 cgggtttggc ggtcggggaa acggcggttt gaatagtcag ggcgcggtcg gcggcggaaa
     1441 tgccggtggt aatgccgtgt gcggcttcga tggagacggt aaagttggtg ccgtattgcg
     1501 cgccgttttt ttgggtcatc atcggcagcc cgagtttttc gaccatttcg ccgtccatcg
     1561 gcaggcagac caagccgcgc gcgtgtttga tcatgaagtt gatggcttcg ggcgtgacga
     1621 attgcgccgc catcagcagg tcgccttcgt tttctcggtc ttcggcatcg gtgatgatga
     1681 ccattttgcc ggctttgatg tcggcaagga tttcggggat gggggaaata tgggacatgg
     1741 tgtgttttcc tgtgtgttcg ggcgggcggg cggtgtgttc ggtatcgagc cagaaggcgg
     1801 gcatttttgc cgcctgttcg attttacggg cttttttctc gccgaaggat ttgtttttca
     1861 aaagggcgga gagttcgcct gtgttgaggg caacggcttc ggcaaaacgg gtttgcaaac
     1921 cgccgtagtg ttctgctatc cattgccgca ggttgcggcg gcgcagaccg gctgtgtcgg
     1981 tcatcgtttc atcctttgta gaaatcggat gtagcggatt gtaatcgcgt atttaccaaa
     2041 aagcaaatct gtttttttcg ccaaaccgaa ttatttgctt tttggtaaac agatgccgtc
     2101 tgaagccgga ttcgtggctt cagacggcat tgccgcaatc cttgccggcc gggggctgtt
     2161 tgtcccaact tcccgcgcac ttctgatttg gggtttgccg gaagcttatt ccgaaaaaaa
     2221 tttctgataa acggccttga ttgccgggta cagcataggc ggtacgagca ggcgcaatat
     2281 cggcaatcct atgatgaatg cggtcttccc gataaaaacc ggacggattg ccttcgcgcc
     2341 gaatatcttt ctgcattttt ctttaaatga aaaatatttc cggtagatga ggataatgtt
     2401 tttggaaaaa taaagcctta ccaatataga cctcagatag ccgatattgt cgaagacaaa
     2461 tttttgcaaa ccgccgtaga tgggggaatt tttgttttcg actaaaaaat ccacaccccg
     2521 gtcgagatgc gtcagcacat cgctgaaatt tttgtatttg atgttgtggc tgatagagcc
     2581 gccgagaacg cggtactggt aaaaagggtt atcgtaaaag gcaaaagtct tgataaaacg
     2641 cgccaattgc aaactgtacg ggaaatcctc gtgaatgtat ccttttggga aaaacagatt
     2701 gtttttgatg attatttccc gcctgacgat ttttgtccac gcgttggcga tatagtaccg
     2761 cccctccacc agcgtttcaa aatggcggac aaaatcatta tccgaaaagt ccgccccttt
     2821 ggggatgtcg cggtaattga aggacgaggg atgcaggatc aaatcaacct ttttgtctgc
     2881 aagttgttgt aaatcaaaga gaatcccccc ccccgcgcgt tttttgaacg attggtgtcg
     2941 gcccaatagt cgtcgctgtc caaaaagatt aggtaatcgc cttttgccgc ccggataccg
     3001 gcgttgcggg catccgacag cccgccgttt tcttgatgaa tcacttttat atgcggatat
     3061 ttgcctgcat attcgtcgca aatcttcccg cagccgtccg gcgaaccgtc atcgaccaaa
     3121 atcatttcat aatcggcaaa attttcggca agcacggaat ccacgcagca gcggaggtat
     3181 ttttccacat tgtaaatagg gacgatgatg gagaaaatca taaatatcaa tccattatat
     3241 taagatgttt gcgcgtatgc ctcaaacccg cacccgcaat gcgtttgcat ccgcaccctg
     3301 taactttata aaacttattg ctttatcaag gtatggaaac ctgtttcccg aaaggcggcg
     3361 caggatgccc gttccctgca tctttgcctt attccgacat cagatgcccc agcttgtctg
     3421 ctttggtttg gaggtagcgt ttgttttcaa ggttttcccc gacgtgcagg gggatgcgtt
     3481 cgaccacgtt aatccccgca tctttcaggg tttggatttt ttcggggttg ttggtcagca
     3541 gtttgaccga gcggatgccc agatattcgt agatagattg cgccaaacgg aaatcgcggg
     3601 catcgacggg cagcccgagt gccaaattgg cttcaaccgt atccatacct tggtcttgca
     3661 gatgataggc gcggattttg ttaatcagcc cgatgccgcg tccttcctga cgcagataga
     3721 cgatgatgcc gcgcccctct gtctgtaccg ccctcatggc cgcttcaagt tgaggtccgc
     3781 agtcgcattt tctcgagaac agcgcatcgc ccgtcaggca ttcggagtgg atgcgcgtca
     3841 gaaccggatt gccgtctgaa aaattaccga cggtcagcgc gacgtgttcc tgcccgtttg
     3901 cctcttcaaa gccgtgcatc gtaaatacgc cccattcggt cggcaggcgg caggatgcca
     3961 catggttcaa catttcagac atcttcactc ccatcttttc cggcagacat acccaacagc
     4021 agtttcaaag gttcggacaa agccaaggca agagccaccc attccgcctg cgctgcctca
     4081 tccgcacact ctgcgcattc aaattccaca tgaaccacgc ccaacacacc gccactttcc
     4141 gtgcagaccg gaatggaaat ttgcgccgcc gaagcatgat tgcgttctcc cgaaagctcc
     4201 cccaaatcca accaacggcg tacatccgag gcaacattca tccaaccgct ttgcgccgaa
     4261 cggcaagcca gcgaaacctt gcctgccgct tcatccaaat cccacaggtt ttccaaaacc
     4321 tcgccctgtc gggacaggca tatcagttgg aaagcctggt tttcagatgg catcaaggca
     4381 tagaccgccg cactctgcac gcctgtcgag cgcgaaaaca ccgaatccaa agccatgaaa
     4441 agccgtttca gtgcatcttc gcggacattg tcgcacgaca ggtaatcggc aagtttccaa
     4501 ccctcatcac tgcgccacaa aaccgaacgg tctatcgaag ccgtccccat atccattacc
     4561 gtctgcgccg tcagatacgc cgcccgaacc tcgtcaagcg gcagcttcaa accctgagtc
     4621 agcagaaaat ccttaatcaa agcagcaggc atacccaaat tccgtcaata aaaacaaaaa
     4681 accgcccgat tccgatgtca ggcggacgaa aaacagattt taccgcccca aaccccaagc
     4741 catcaacagc cccgccgaaa accttgcaca aaaaaacaaa atctgtataa tcgtccaatt
     4801 cccatgcgga cgtggcgaaa ttggtagacg caccagattt aggttctggc gccgagaggt
     4861 gtgagagttc gagtctctcc gtccgcacca ataatgataa ataaattata tagttaatgc
     4921 ttatcgggac aaattagtgc cacaaaacat aagtcgtctg cttttcagcg ggcggctttt
     4981 ttatctgcct atatgtttca acacgcagaa cggtacataa agtgccgcca tttgttccgc
     5041 accgaatcgg aaacgggtgc ttccgcccgc aatcagattc accgccccta atgccggggt
     5101 ttcaaccgga aagaagaaaa tattgatttt gatgatggca tcaaaaaccc ccttccgctc
     5161 gtctgccaaa aggaatggtt aagaaaagca atgcgtcttt atgatggaac gcgatatata
     5221 aggaattgag ggaaagggca ggcgggtttc tgcccgttaa tcctgcggac ggtcccgcca
     5281 aacccgacac gcgcgggcat atggcggact gtttacaata ttgtctaaaa atctgaacct
     5341 gcaaacgtga aataaggtag aatacgcccc gttattttac cgtccaatcc ccatttacca
     5401 aggaaaaacg atgagtactt cattgagtta ccgcgatgca ggtgtcgata tcgacgcagg
     5461 cgaccaactg gtcgaaaaca tcaaaccgtt tgccaaacgc accatgcgtc cggaagtatt
     5521 gggggatttg ggcggttttg gcgcattggt cgaaatcggc aagaaatata aaaatcccgt
     5581 attggtcagc ggtacggacg gcgtgggtac caagctcaaa cttgcctttg attgggataa
     5641 acacgatacg gtgggcatcg accttgttgc aatgagtgtc aacgatattt tggttcaagg
     5701 agcagaacct ctgtttttct tggattattt cgcctgcggc gaattggatg ttccgcgcgc
     5761 aaccgatgtc atcaaaggca ttgcacaagg ttgcgaagaa tccggctgcg ccctgattgg
     5821 cggggaaact gccgaaatgc cgggtatgta tcccgtcgga gaatacgatt tggcgggttt
     5881 tgccgtcggc gtagtggaaa aagagaatgt cattaccggc cgcagcatcg gcgcgggcga
     5941 tgtggtattg ggtttggctt ccaacggcgc acattcaaac ggctattccc ttatccgtaa
     6001 aatcatcgaa cgcgacaatc ccgatttgga tgccgagttt gataatggca aaaccttgcg
     6061 tgaggctgtt attgcgccga cccgtctgta tgtgaaaccg attcttgccg ctttggaaaa
     6121 atttaccatt aaaggtatgg cacacattac cggcggcggc attaccgaaa acgtgccgcg
     6181 cgtgttgcct gaaaacacgg ttgcacaaat cgatgctaaa tcgtgggaat tgcccaagct
     6241 cttccaatgg cttcaaaagg cgggcaatgt ggaaacccaa gaaatgtacc gaacctttaa
     6301 ctgcggcatc ggcatggtcg ttattgttgc tgccgaagat gccgatgcgg ttcaggatct
     6361 cttgggtgaa caaggtgaaa cagtttaccg tttaggtttg atccgtgagc gtcagggaga
     6421 cgagcatcaa acccaggttg cctgattgct tctatagcga aatgccgtct caaacatggg
     6481 tttggacggc atttttatag
//
LOCUS       DAWFAD010000107         6434 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWFAD010000107 DAWFAD010000000
VERSION     DAWFAD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.107"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            109..1650
                     /locus_tag="WGO49_001555"
     CDS             109..1650
                     /locus_tag="WGO49_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ0306649.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAAMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGWQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRACSDKAVSPVMPSEPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVFAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDGGEGGK
                     RTETL"
     gene            1679..2278
                     /locus_tag="WGO49_001556"
     CDS             1679..2278
                     /locus_tag="WGO49_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ0306650.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESRIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2275..2880
                     /gene="nadD"
                     /locus_tag="WGO49_001557"
     CDS             2275..2880
                     /gene="nadD"
                     /locus_tag="WGO49_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ0306651.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRNLASQGVSDGIPPAAARYIREHGLYEK"
     gene            2939..3325
                     /gene="rsfS"
                     /locus_tag="WGO49_001558"
     CDS             2939..3325
                     /gene="rsfS"
                     /locus_tag="WGO49_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ0306652.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3379..3849
                     /gene="rlmH"
                     /locus_tag="WGO49_001559"
     CDS             3379..3849
                     /gene="rlmH"
                     /locus_tag="WGO49_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ0306653.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYVLKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAIPQGAFLVVLDERGKAPTSVELAEHLKTWQQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            3924..4190
                     /locus_tag="WGO49_001560"
     CDS             3924..4190
                     /locus_tag="WGO49_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ0306654.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4347..5030
                     /locus_tag="WGO49_001561"
     CDS             4347..5030
                     /locus_tag="WGO49_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ0306655.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5154..5666
                     /gene="coaD"
                     /locus_tag="WGO49_001562"
     CDS             5154..5666
                     /gene="coaD"
                     /locus_tag="WGO49_001562"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ0306656.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5698..6351
                     /locus_tag="WGO49_001563"
     CDS             5698..6351
                     /locus_tag="WGO49_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ0306657.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNRESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1402 a         1708 c         1859 g         1465 t
ORIGIN      
        1 ttcagacggc gggaggaagg cggatacggt atgtagtcgg attgaaatat acggtatgtg
       61 gggcttttgg gtacaatcgc aacatcagtg tttcaagtgg ggaaggcgat ggatggacgg
      121 cgttgggcgg tatggggtgc ttttgccctg ctgccttcgg cttttttggc ggcaatggtc
      181 gttgcgcctt tgtgggcggt ggcggcgtat gacggtttgg cgtggcgcgc ggtgctgtcg
      241 gatgcctata tgctcaaacg tttggcgtgg acggtatttc aggcagcggc aacctgtgtg
      301 ctggtgctgc ctttgggcgt gcctgtcgcg tgggtgctgg cgcggctggc gtttccgggg
      361 cgggctttgg tgctgcgcct gctgatgctg ccgtttgtga tgcccacgtt ggtggcgggc
      421 gtgggcgtgc tggctctgtt cggggcggac ggtctgttgt ggcgcggctg gcaggatacg
      481 ccgtatctgt tgttgtacgg caatgtgttt tttaacctgc ccgtgctggt cagggcggcg
      541 tatcaggggt ttgtgcaagt gcctgcggca cggcttcaga cggcacggac gttgggcgcg
      601 ggggcgtggc ggcggttttg ggacattgaa atgcccgttt tgcgcccgtg gcttgccggc
      661 ggcgtgtgcc ttgtcttttt gtattgtttt tccgggttcg ggctggcatt gctgctgggc
      721 ggcagccgtt atgccacagt cgaagtggaa atttaccagt tggtcatgtt cgaactcgat
      781 atggcggttg cttcggtgct ggtgtggctg gtgttggggg taacggcggc ggcagggttg
      841 ctgtatgcgt ggttcggcag acgcgcgtgt tcggataagg cagtttctcc tgtgatgccg
      901 tctgaaccgc agtcggtcgg ggaatatgtg ctgctggcgt ttgcggcggc ggtgttgtct
      961 gtgtgctgcc tgtttccttt gttggcaatt gttgtgaaag cgtggtcggc cggcgaatcg
     1021 tggcgtgtgt taatggaaag tgaaacgtgg caggcggtgt ggaatacttt gcgcttctcg
     1081 gcggcggcgg tgtttgcggc ggcggttttg ggtgtggtgt atgcggcggc ggcgcggcgg
     1141 tcggcgtgga tgcgcgggct gatgtttttg ccgtttatgg tgtcgccggt ttgtgtttcg
     1201 gcgggcgtgc tgctgcttta tccgcagtgg acggcttcgt tgccgttgct gctggcgatg
     1261 tatgcgctgc tggcgtatcc gtttgtggca aaagatgttt tgtcggcttg ggacgcactg
     1321 ccgccggatt acggcagggc ggcggcgggt ttgggcgcaa acggctttca gacggcatgc
     1381 cgcatcacgt tccccctctt gaaaccagcg ttgcggcgtg gtctgacttt ggcggcggca
     1441 acctgcgtgg gcgaatttgc ggcaaccttg ttcctgtcgc gtcccgagtg gcagacgctg
     1501 acgactttga tttatgccta tttgggacgc gcgggtgagg ataattacgc gcgggcgatg
     1561 gtgctgacat tgctgttggc ggcgttcgcg ctgggtattt tcctgctgtt ggacggcggc
     1621 gaaggcggaa aacggacgga aacgttataa tgcgaaccct ttttcagagg acggcaaaat
     1681 gagcgagttg gataacatcc ttgcccataa ccggcagttt gtcgagtcgg gcgaatatga
     1741 aaaatacttt accgacaaat accccgaacg cgggctggca gttttgtcct gtatggatgc
     1801 gcggattatc gggctgctgc ccgacgcgtt gggtttgaaa aacggcgatg ccaagctgat
     1861 taaaaatgcc ggcgcgctgg ttacgcaccc gtggggttcg gtgatgcgga gccttttggt
     1921 tgccgtgttt gaactgaagg tcagagagat tatggtcatc gcccatcacg attgcggtat
     1981 gcaggggctg aatgccgaag aattcctcgg gcgcgtccgg gaaagccgga ttcccgaaga
     2041 ccgtatcgaa accctgcgtt atgccggtat cgacctcgac ggctggctga ccggtttcga
     2101 caacgtcgaa gacagcgtgc gccacacggt ggaccttatc cgtaaccatc cgctgatgcc
     2161 gcgccatatc gccgttcacg gattggtcat ccatcccgtt accggcaaac tgacgctggt
     2221 tgtggacggc agtgtttcag acggcatgga cttatcggaa ggaatggaaa catcatgaag
     2281 aaaatcggac tgttcggcgg cactttcgac ccgatacaca acgggcatct ccacatcgcc
     2341 cgcgcctttg ccgacgaaat cggcttggac gcggttgttt tcctgccggc aggcggcccg
     2401 tatcacaaag acgcagcctc cgcttccgcc gccgaccgcc ttgccatggt cgaactggcg
     2461 acggcagaag acgcgcgttt tgccgtcagc gattgcgaca tcgtccgaga aggtgcaacc
     2521 tatacttttg ataccgtcca aatcttccgc cagcagttcc catccgcgca actctggtgg
     2581 ctgatgggca gcgacagcct gatgaagctg cacacatgga aaaaatggca gatgctcgtg
     2641 cgcgaaacca atatcgccgt cgccatgagg cagggcgaca gcctgcacca aaccccgcgc
     2701 gaactgcacg cgtggctggg caagtccctt caggacggca gcgtccgcat cttgtccgcc
     2761 ccgatgcata atgtgtcgtc aacggaaatc cgccgcaacc ttgccagcca aggcgtttca
     2821 gacggcatcc cgcctgccgc cgcacgctac atccgcgaac acggtttgta tgaaaaataa
     2881 agtcaaatca gtaagaaacg gctataatgc cgtctgaaaa catcccgtta ggaaaataat
     2941 gaacgaacaa gaactgcaag acctgcaaaa aatggtcggg gtcgccgtca acgccctcga
     3001 agacatcaaa gccaaagaca tttccgttct cgaaacgcaa gacaaaactt cgctgtttgc
     3061 cagaatgatt atcgccagcg gcgacagtac gcgccaagtc aaagcactgg ccaacaacgt
     3121 tgccgtcgat ttgaaagaag ccggttttga aatcctcagc accgaaggcg acagcggcga
     3181 atggacgttg gttgatgcag gagacctcgt cgtccacgtt atgctccccg ccgtgcgcga
     3241 cttctatgat atcgacaccc tttggggcgg cgagaaaccg agtttccacg ccggaatgca
     3301 gaagccgtgg cacgcggcag actgattccc gatgccgtct gaatgttcag acggcatttt
     3361 cctgtaaggg agaaagcatt gaacatcacc gttttggcag tcggcaccaa aatgccgcgc
     3421 tgggttgatg aggccgtcgc cgaatacgcc aaacgcttcg gacgcgacgt cgcctacgta
     3481 ctcaaagaaa tcaaacccga aaaacgcggc gcgggcgtga atgccgccca aggaatggcg
     3541 gcggaagaaa aacgcatcct cgaagccata ccgcaaggcg cgttcctcgt cgtccttgac
     3601 gaacgcggca aagcaccgac ctccgtcgag ctggcggaac acctcaaaac ttggcagcaa
     3661 aacggcgaac acgtctgctt cgtcatcggc ggcgcggacg gcatgaccga ccgcctcaaa
     3721 cagcaggccc gcatgatgat gcgcctgtcc agcctgaccc tgccgcacgg gatggtgcgc
     3781 gtccttctga ccgaacaact ctaccgcgcc gcctccatcc tgcacaacca cccctaccat
     3841 cgggaataag aaggcttttg tccgcactcc caatcggtta aaatcccccc gttatttctg
     3901 aacacacagc aaaggaatcc gctatggcac gcatggtatt ctgcgtcaag ctcaacaaag
     3961 aagccgaagg catgaaattt ccgccgcttc ccaacgaatt gggcaagcgc atttttgaaa
     4021 acgtatcgca agaagcgtgg gcggcgtgga cgcgccacca aacgatgctg attaacgaaa
     4081 accgtttaag cctcgccgat ccgcgcgcgc gcgaatacct ggctcagcag atggagcagt
     4141 atttcttcgg cgacggcgcg gatgccgttc agggatacgt tccgcaataa cggttttccg
     4201 ttttgaacac aggctgtccg aaactgcttc agacggcctt taaaatacgc cggacaactt
     4261 tatcttcacg caaccggaca aattttgaca ttgggcaatg ttaaaatccc ccattattcc
     4321 caaccgcgct ttcaggagca gatgatatgc aacacgacgt ttacgactac accgcgcata
     4381 cggtttctaa aaacaccgtc ctgcagaaaa cctaccgcct gctcggattt tcattcattc
     4441 cggcagccgc aggcgcggca cttgccgcca atgccggttt caatttttac gccgccttcg
     4501 gttcgcgctg gatagggttt gccgtcgtgt tggcgttttt ctacggtatg atccacttca
     4561 tcgagaaaaa ccgttacagc aataccggcg ttaccctgct gatggtattc acattcggta
     4621 tgggcgtatt gatcggcccc gtgctgcaat acgcgctcca tattgccgac ggtgcgaaaa
     4681 tcgtcggcat tgccgccgcg atgaccgccg ccgtcttttt aacgatgtcc gccttggcgc
     4741 gccgaacccg gctcgatatg aacgcgctcg gacgcttcct gaccgtaggt gcggtcattc
     4801 tgatggtcgc cgtggttgcc aatctgtttt tgggtattcc cgcactcgcc ctgaccattt
     4861 ccgccggttt tgtcttgttc agttccttaa tgattatgtg gcaggtacgc accgtcatcg
     4921 acggcggcga agacagccac atcagcgcgg cactgacact gtttatctcg ctttacaaca
     4981 tcttcagcag cctgctgaac atcctgttgt ctttaaacgg cgaggattga tgccggtaaa
     5041 acgccgtccg gctatgccgt ctgaaaatgc ttcagacggc attttacttt gggcatacaa
     5101 tacggatgca cacgcaccaa cacacagccg gctatgaggc cgggaggaat caaatgttac
     5161 cgaatacacc gcgccgcgcc gtttatgccg gcagtttcga tccgcccaca ttggggcatc
     5221 tgtggatgat acggcaggcg caatctatgt ttgacgaact catcgtcgcc atcggcatta
     5281 accccgacaa acgcagcacc tataccgtcg ctgaaaggca ggatatgttg tgcgatatta
     5341 ctaaaatgtt tcccaacgtc agaaccgatg tatttgaaaa ccgatttctg gtgcattatg
     5401 cccgtgaggt agatgcagga ttcatcgtgc gcggcatccg ttctgcttcg gattacgaat
     5461 acgaacgttc catgcgccac atcaacagcg acctcgcccc cgaaatatcc accgtattcc
     5521 tcatgccgcc gcgcgaaatc gccgaagtgt cgtccactat ggtcaaagga ctggtcgggc
     5581 cggaaggctg gacggaaacg atccaccgct acgtgccgca agctgtgtac gaaaaaatcc
     5641 ttgccgaaca tcaacacgaa aattgaaccc ttctattttt gcaaggcata tatggatatg
     5701 ttggaaattt tgttccgcca tcaagacttc gtcgccatca acaaaccggg cggcatatcc
     5761 gtccaccaag acagcggcga gaccggactg gcccgaacac tcgccataca gttgggcgta
     5821 gagcgtgtgt ggctgctgca ccgattggac aaacagacca gcggcatttt actgtttgcc
     5881 ctcaacagag aaagcgcgtc cgcccttagc ggacagtttg ccggcaaaag catcaaaaaa
     5941 acctatttgg cactatccga ccgcaaaccg tccaaaaagc agggttggat taaaggcgga
     6001 atggaaaaat ccagacgcgg aatgtggaag ttgacgcgca atacagaaaa tatcgccgtt
     6061 acccgattcc acagcatcag tatcgccgaa aaactgcggc tgttcatcct ggaaccgcat
     6121 acgggcaaaa cgcaccaatt gagagtggcg atgaaaagtt tgggcagtcc gattctgggc
     6181 gacagcttat acggcggaac agaatccgaa accatgtttc tgtatgcatg gaaaatacaa
     6241 tttgcctacc aaaaccgaca aatcgaaatt gttgcaccct taaaaaacga atggcagact
     6301 gaaaatatct ctcatgcgct ggaagaattt tatatggcaa aagcaggtta ataaatataa
     6361 attaatttat aaacaatatg ataaatattt ttataaaaat ttttcaagaa aacggttgac
     6421 tgcaaatctc ttca
//
LOCUS       DAWFAD010000108         6416 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWFAD010000108 DAWFAD010000000
VERSION     DAWFAD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.108"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            175..1233
                     /gene="alr"
                     /locus_tag="WGO49_001564"
     CDS             175..1233
                     /gene="alr"
                     /locus_tag="WGO49_001564"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ0306658.1"
                     /translation="MRPLNVQIRLGNLRHNYQILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLIRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1297..2652)
                     /locus_tag="WGO49_001565"
     CDS             complement(1297..2652)
                     /locus_tag="WGO49_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ0306659.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAASKADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVNRGGRIPAPVQSMK
                     N"
     gene            complement(2668..2940)
                     /locus_tag="WGO49_001566"
     CDS             complement(2668..2940)
                     /locus_tag="WGO49_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ0306660.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3066..3803)
                     /locus_tag="WGO49_001567"
     CDS             complement(3066..3803)
                     /locus_tag="WGO49_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ0306661.1"
                     /translation="MKKLSRIVFSTVLLGFSAALPAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAHAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGGKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIRHHQRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4084..4983)
                     /gene="prmB"
                     /locus_tag="WGO49_001568"
     CDS             complement(4084..4983)
                     /gene="prmB"
                     /locus_tag="WGO49_001568"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0306662.1"
                     /translation="MFNQAAQELTTIRDILRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLAVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5128..5721
                     /locus_tag="WGO49_001569"
     CDS             5128..5721
                     /locus_tag="WGO49_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306663.1"
                     /translation="MADGMGNLIGWEKTGLVVGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNKSLNIGVIVREFPDDHKHYDHFILKDE"
     gene            5833..>6416
                     /locus_tag="WGO49_001570"
     CDS             5833..>6416
                     /locus_tag="WGO49_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ0306664.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIAAAAMKA
                     AIPDYMKFGAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVERPANPDAVSTCFKADKGAETVNNDTEHNAT
                     YFSHFPSKQKSTDGGRCPFLYRFPYFLSAGRAGFA"
BASE COUNT         1718 a         1620 c         1499 g         1579 t
ORIGIN      
        1 tttgggaaag cgattatatc aggaaaagca aaccgccttc ctacctgaaa actgctgctt
       61 cggcttgaag acacaagggt ctttaatatt ttaaaagcct tgccgttgga ttataattcc
      121 ctaattgatt tcttaatttt gctaataaac acttgtttgg taaggaatga atttatgcgt
      181 cctttgaacg tgcagatcag gttgggcaac cttaggcaca attatcagat tttaaaggaa
      241 atgcacggag gcaagctgtt ggcggtagtg aaggccgacg catacggaca cggtgcggtc
      301 agatgtgctt tcgcgctggc agacttggca gacggctttg ccgtggcgac aatcgatgaa
      361 ggaatcaggc tgcgggagag cggcattacc catccgattg tccttttgga aggcgtattt
      421 gaagcatcag agtacgaagc ggtcgaacaa tactcgcttt ggccggcagt cggaaaccaa
      481 tggcagcttg aggctttgct gatccgccat tggaaaaaac ccgtcaaagt ctggttgaaa
      541 atggattcgg ggatgcaccg taccggtttt ttccctcatg attacgcttc ggcatatgcg
      601 gcattgaaac agtcggaata tgtggacagt attgtcaaat tctcgcattt ctcctgtgcg
      661 gacgaacccg aaagcggtat gacggaaata cagatggaag cattcgattt gggtacggaa
      721 gggctggaag gcgaagaaag ccttgccaat tcggcggcta ttttgaatgt tcccgaagca
      781 cgcagggatt gggggcgcgc gggcttggcg ttatacggca tttccccgtt cggaggcagc
      841 gatgacaggc tgaaacccgt gatgaggctt tcaacccgta tttttggcga acgcgtttta
      901 cagccgcact cccctatcgg ttatggcgca acattttata ccagcaaatc tacgcgcgtc
      961 ggtctgattg cctgcggtta tgcggacggt tatccgcgcc gcgccccaag caattccccc
     1021 gtcgctgtcg acggcaaatt gacccgggtc atcggcaggg tctctatgga tatgatgacc
     1081 atcgagctgg acgcttcgca agaaggtttg ggacacgagg tcgaactgtg gggcgatacg
     1141 gtcaacatca atactgttgc cgaagcggcc ggaaccatcc cttacgaatt gatgtgcaat
     1201 atcaaacgtg caaaattcac ttatatcgag taatcaagtc caaacaaaaa tgccgtctga
     1261 agcctttcag acggcatttc cccatcaaaa ccgcaatcag tttttcatcg attgaaccgg
     1321 tgcgggaatc ctgccgcccc ggttgacgaa cacttcgcat gagccttctt ttaccggcat
     1381 tacaggcgcg tagcccaaca ggccgccgaa ctcgacgctg tcgccgacgg tttttccggt
     1441 taccggaata atgcgcacgg cagtggtttt gctgttgatc atgccgatgg cggcttcgtc
     1501 ggcgatgatg ccggaaatgg tgtgtgcggg cgtatcgcca gggacggcaa tcatgtccag
     1561 cccgaccgag cagacggcgg tcatcgcttc gagtttatcc aacgtcagca cgcctgcttc
     1621 ggcggcggca atcatacctt cgtcttcgga aacggggata aacgcgccac tcaaaccgcc
     1681 aaccgcgctc gaagccatca tgccgccctt tttcacggca tcgttcagca atgccaaagc
     1741 tgctgttgtg ccgtgcgtac cgcagacgct caaacccatt tcttcaagaa tgcgcgccac
     1801 cgagtcgccg acggcagggg tcggtgccag cgacaagtcg agaataccaa acgggatatt
     1861 cagcattttt gaggcttcgc ggccgatgag ttcgcccacg cgggtaattt tgaaggcggt
     1921 tttcttcaca acttcggcaa cttcggtcaa tgtcgttgca tccgaatttt ccaacgcggc
     1981 ttttacgaca cctgggccgg atacgccgac attaatcaca gcatccgctt cgcccgatcc
     2041 gtgaaacgca cccgccataa acggattgtc ttccaccgca ttgcagaaca cgacgatttt
     2101 cgcgcagccg aaaccttcgg gcgtaatttc agccgtgcgt ttgatggttt cgcctgccag
     2161 cttgaccgca tccatattga taccggcgcg cgtactgccg acattgatgg agctgcacac
     2221 gatgtcggtg gtcttcatcg cttcgggaat ggaacggatt aacacctcgt ctgaaggcga
     2281 catgcctttt tgcaccaacg cggaaaagcc gccgataaag gacacgccga tggctttggc
     2341 agccttatcc aaagtttgcg ccacgctgac gtaagaatcg gctttgctcg ccgccgcgat
     2401 ttgggcaatc ggcgtaacgg aaatgcgctg attcacaatc ggcacgccgt atttggcaga
     2461 cagatatttt gccgtcgcca ccaagtcttt gccgaccgtg gtgattttgt tgtaaatgtt
     2521 ttggtttaac acgtcgatgt cggtgctgat gcagtcgtgc aaatcaatgc cgatggtaat
     2581 ggtgcgcacg tcaaaattct ggtcggccac cattttgacg gtttctaaaa tctcgccgga
     2641 ttgaatactc atggctctcc ctccgactta aatacggtgc atggcttgga aaatttcttc
     2701 gttttgcata cggatatcaa gcgcgagttt tttgctctct tccgcaaaca aatccaaaac
     2761 ctcctgccga gatttggggc atttcgaggt atccaccaag ataatcatgg taaaaaaatc
     2821 gtccatcagc tgttggctga tattaaggat gttgattcgg ttttccgcca aaattttgga
     2881 aacatcgtac acgatgccga cgcggtcttt gccgatgacg gtgatgactg aattgttcac
     2941 aggcttactc cttgcagata tccgttaaag tccgaaatta taccaccgtt ggattttgaa
     3001 gaaatattgt caacaatata tacatacaaa atgccgtctg aaactatttc agacagcatc
     3061 aagattcagg gttcgattaa ataaccatcc ttatcccact gggttttcct gaccaacttg
     3121 tcatcctgat aaacagcctc gctcttttta gaaccatctt cataccactc caaaaccacc
     3181 ccgttgcgtt gatggtggcg gatagacagt tccgagagta atcggccgct ttcatcccaa
     3241 gtcagaattt tggcaggctc atcgttgacc ataaccattt ccgttttgat attgccgtcg
     3301 gcataccatt gcttccatac gccgtttgcc ttattctgtt taaactggat ttcgctttcc
     3361 ttgccgccgt tacggtaata gcggtacccc gtaccttcac tcaaaccatt tttataaggc
     3421 ataacggcag attttttacc gttcggatac cagttgaccc actccccgtc cggcttaccc
     3481 ttgctgaagc cccccgccat ttttttctga ccattaaaat gccacaaaat caacataccg
     3541 ttttgcaggg taggcacaaa agatttgatt tgcgttgaag caacgatata aggttcggaa
     3601 tatttcttca tcgacggata ataaaaatcc tgcgcgtgcg caataccctc cgccacacta
     3661 tattgcctga tataagcggc agaagacatc gtcgccgtca gtttcccgtt ctgattaaaa
     3721 taaacagaat aggtctgcgc cggcaaagcg gccgaaaaac ccaacaggac agttgaaaat
     3781 acaatccgag ataatttttt cattgcaata gcgatataaa aacaaggctg tgttttagta
     3841 atcttttgat ttcaattatt tgcaagggaa aagacaatta ttttccggtt aggaataaac
     3901 ctattctatt gaatatattg aagccaagta cgcctatcaa cactatatta aaacactgcc
     3961 aaaaacaatt aacttataaa caatatggta aggatttctc tgccaagcat caaacccgag
     4021 acaacgtatc gtaaaaatgc cgtctgaaaa caaatcgtct tcagacggca tttccccttc
     4081 aactcactct tcacccaata actgctcgcg cgtcaagagg aaaacaaaac cgtcgccccc
     4141 gctggtttcc aaccaagtaa aaggcaactc cggatacgct gcttccaata catccctgtt
     4201 atgcccgatt tccaccagca atacaccttt gggattcaga aactttgccg cattcagaag
     4261 aatctgcctg gtggcatcca acccgtccgc cccgctgccc aatgccaatt ccggttcgtg
     4321 caaatactct tcaggcaata actcaaccga ttccgcatcc acataaggcg gattggaaac
     4381 aatcaaatca taagtgcctt ccaatccttc aaacaaatcc gtatgaataa gccggatgcg
     4441 ttcttccaaa ccataatctt cgacattaat ccctgccact tccaaagcat ccaagctcac
     4501 atcaaccgca tcaatttggg catcaggata atgatgcgcc atctgaatgg caaggcaacc
     4561 gcttccggtg caaagatcca aagcattatg caccagctca tcgtattcta tccaaggacg
     4621 aagtccgtta cccaacaatt cataaataaa agaacgaggt atgattacgc gctcatccac
     4681 atagaaatca aactctccct gccatgcctg gtgtgtcaaa taagcggctg gaatgtgttc
     4741 gacagcacga cgctcaataa ccgccagcac ttcctctttt tcagcttcca agagttttgc
     4801 atcaagatat ggggcaagca tatccaaagg caaattcaaa gtatgcagaa tcaaataagc
     4861 cgcttcatca tgcgcattat ctgttccatg accaaaaaag agccctgcct cattaaaacg
     4921 gctgactgca aaacgtaaaa tatcgcggat agtcgtcaat tcttgtgctg cctgattaaa
     4981 cataatatga accattctgc gtatagatac ttttaattat aacagaaaca acaagcaaac
     5041 cttttcatat cgccaaataa ccacccaatc tacccataca actacataaa tgcccgcgcg
     5101 aaaaccatcg cccgaacgga aacgacaatg gccgacggta tgggcaatct gattggctgg
     5161 gaaaaaacgg ggcttgttgt cggtaagcag tggataaccg caaaagacga caaggtgtcc
     5221 gatgtctgca atgccaacgg cgagatgggc gtaatcgggc tttacgagcc tttctcacac
     5281 ggcgcattga cgatacccgg tcatccgaac tgccgatgcg aggttgtttc cgtatcgggt
     5341 ggcgaattgg gggaatttgc cgaaaaaaag gagcttcgta aagcggctat gcagtatgcg
     5401 cgggataact ttatcggcaa aagctatgtc aatgaaaaca gcgggcatga actgaaggta
     5461 acttggcaag gtgtgaaaca cgctgcgtca aaggcaaatc aggcggaatt atccatcatg
     5521 acaaaacttg atgacttatt gcgctacgca aaatatgagg gttcttattc ggataggaaa
     5581 ggtcatccta atattattgc agcacataag tatcgtgccg ttgccaaggt tgggaataag
     5641 tctttaaata tcggtgtgat tgtaagggaa tttccagacg accataaaca ttacgaccat
     5701 ttcatcttga aggatgaata aagccctttt gcagtgtcgt tctggagcgg atagcgttaa
     5761 ggcaagtaca cttccagcct tgaaaaaggg ctttaaattc agcatgccat ttatacaggc
     5821 aggagtaaac ccatgacaaa gttatacgca gaaatcgcca agatggagac gcaggacgac
     5881 gacacggtca aggtttgggg ttacgcttca agcgaggaaa tcgattcgga cggcgaagtc
     5941 atcgcggcgg cagctatgaa ggcggcgatt cccgattata tgaagtttgg cgcggggcgc
     6001 gagatgcacg gctcaaacgc tgcgggaacg gcaattgaaa tcaacgttga agacgacggc
     6061 ataacctttt tcggtgctca tatcatcgac cccgttgtcg tgtcgaaagt caaaacaggc
     6121 gtttacaaag gcttttccat cggcggcagc gttaccgccc gcgatgattt gaacaagtcg
     6181 caaatcacgg gcttgaagct gacggaaatc agccttgtcg agcgccctgc caatcccgat
     6241 gcggtgtcta cctgctttaa ggcggacaaa ggtgcggaaa cggtaaacaa cgatacagaa
     6301 cataatgcta catattttag ccatttccct tccaaacaaa aaagcaccga cggcggccga
     6361 tgccccttcc tttacaggtt cccctatttt ttatccgcgg gcagagccgg ttttgc
//
LOCUS       DAWFAD010000109         6255 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWFAD010000109 DAWFAD010000000
VERSION     DAWFAD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.109"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(391..825)
                     /locus_tag="WGO49_001571"
     CDS             complement(391..825)
                     /locus_tag="WGO49_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ0306665.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGO49_001572"
     CDS             complement(828..1490)
                     /gene="exbB"
                     /locus_tag="WGO49_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ0306666.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1556..2392)
                     /locus_tag="WGO49_001573"
     CDS             complement(1556..2392)
                     /locus_tag="WGO49_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ0306667.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     ETGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMENDE
                     QGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVKFE
                     LN"
     gene            2647..3285
                     /locus_tag="WGO49_001574"
     CDS             2647..3285
                     /locus_tag="WGO49_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ0306668.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3737..4537)
                     /locus_tag="WGO49_001575"
     CDS             complement(3737..4537)
                     /locus_tag="WGO49_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ0306669.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(5516..6157)
                     /locus_tag="WGO49_001576"
     CDS             complement(5516..6157)
                     /locus_tag="WGO49_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ0306670.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMMMSEKSGVPLYFDRAL"
BASE COUNT         1676 a         1565 c         1288 g         1726 t
ORIGIN      
        1 ggtatagcgg attaacaaaa accagtacgg cgttgcctcg ccttaactca aagagaacga
       61 ttctctaagg tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtctacggc
      121 ttcgttgcct tgtcctgatt tttgttaatc cactataaaa attagaaatg cacattttca
      181 ttattctcgc gcaggcagga ctccagactt acccatttca gtaatgtttg aaaataaaag
      241 aaaaatcaga tgtttgtatt cccgtctgcg cagaaatgga gacggcgctc tgtcgtctca
      301 tttttgtttt aatcaactat atatagctga ttaaacataa gaaatgccgt ctgaaagact
      361 ttcagacggc attcgttcaa gcgtcgaact ttattgcgcc ttggtttcgg ttacaaaacc
      421 gattttggtg attcctgcct gacgggcggc ttctaaagct ttgtttacat aatcgtattc
      481 caccgccttg tctgccgcaa tcgccacaat cacgttttca ttctgctcct tggcggcttt
      541 cagacggctt tccacttccc cgatttccac tttgcttgca gaatccccgc cgacataata
      601 gccgccgttc gcatcaatcg tcaggcgcag ggggtcttta ggctgtttgt cctgcttgtt
      661 tgtctgctcg gacgcggtcg gcagttccaa agggatggaa tgcgtcagca ccggcatagt
      721 aatcataaac acaatcagca acaccagcat cacgtccacc aacggcgtaa cgttgatgtc
      781 ggacatcgga gaatcgtcgc cggaattcat cgaaccaaat gccataatca gctatccttt
      841 tgattaagca ggcggacgtg caaatcgtgc gccatcgcat ccaaatcctg ggtcagtatt
      901 tttgtgccgc gattgaggaa gttgtatgcc aacaccgccg gaatcgccac gaacaaaccc
      961 gccgccgtcg ccaccagtgc ctcgccaatc gggccggcaa ccgccgcaat actcatctgc
     1021 ccgctttgcc cgatattgat cagggcgtgg taaatccccc aaaccgtgcc gaacagcccg
     1081 ataaacggcg cggtcgcgcc gatggaggca agcgcggtca tcccgtaatc aaaccggcgc
     1141 ataatctgcg ccatactgtt gcggatttga atgaccaaat actcgttcaa cggcaaagcc
     1201 tgcgccagtt cggacgcttc gtttcggcgg tagttgcggt aagactgcaa tgcctcttgc
     1261 gccagtttgg acaaaggcgc atcgacggcg cgcacttttt cgaccgcgtc gttcagcgac
     1321 aaagtatcgc gcatatgccg tttgacggcg gcattccctt tgcgcgcccg atacagcttg
     1381 atgcagcgca agacaaccaa acaccacgtt acgatactca tcaacagcat caacacaaac
     1441 acaccgatca ggacgggatc gcccgattca aatactaatt tcaaattcat aatgattcca
     1501 acactgaaaa aaccaatcaa acatccaagc tgccgcaaac cgctgcggca accgcctaat
     1561 tcaattcaaa cttgacgggg actttaaact ccgtccaggc attggcttga aaatgcccgt
     1621 tttgcgccgc cttgcgtgcc gcattgtcca accgagaaaa accactgctt ttcacgattt
     1681 taacggactc gacatgaccg cccggagaaa ccaaaacgct caaaacaacc gtaccctgct
     1741 cgtcattctc catagaaagc gtgggataag ccgggcgcgg aatgctgccg ttggcgcgta
     1801 aaggattgcc tttgctgctg ccggctcctt ccccgtgttc gcctttgaca ccgccgctac
     1861 ctttaccgct gccttctccg cgccccgttc cgtctccttt ggtaccggtt tcttccccat
     1921 taccctgctc gctgtctgct ttggcagaag cattgctggg atgttcggca ggtttttcag
     1981 acggcttctc gaccggtttt tccgccggtt tcgggacagg cttcgcttcc ggcttaggct
     2041 ctggtttcgg tttttcttcg ggtttcggct tttcttcagg tttcggctct tccttaggct
     2101 gctgaatatc cgcatccgcc tttttcgtaa ccaccggctt caaaaccggc ttgggcggct
     2161 cgacaggttt gggcggctca ggctcaggtt gcggctcagg cgcagcaggc gcacctgcgc
     2221 cttcgggtgc accgtcccct ccgccaaaat cacccaaatc gacaaattcg ataacatttc
     2281 ctgactctat cacgggcagc ttgtgcgcct gccagagcaa tgccaccatt gccaaatgca
     2341 gcagtgcgac ggaaaacacg actgcggggg ttaaaattcg ttctttatcc ataattcggg
     2401 cataataata gcaacaattc ctatttgcaa cctattttta caatttttgg tcatatgaat
     2461 gtctgttccg ttcacaggca aaccgtgttt aaacgctgta ttacagcaaa tcatcagata
     2521 acgggccggc agaaaaaatg attccgtctg atttcttatt ccaataaaat caggttagat
     2581 gatatattgc cgcttctgtc tgtcagccgt ttcgggctgc acaccacatc tgttcaaagg
     2641 aaaaccatgt ttcaaaattt tgatttgggc gtgtttctgc ttgccgtcct gcccgtgctg
     2701 ctcgccatta ccgtcaggga ggtggcgcgc ggctatacgg cgcgctactg gggagacaac
     2761 actgccgaac aatacggcag gctgacactg aaccccctgc cccatatcga tttggtcggc
     2821 acaatcatcg taccgctgct tactttgatg ttcacgccct tcctgttcgg ctgggcgcgt
     2881 ccgattccta tcgattcgcg caacttccgc aacccgcgcc ttgcctggcg ttgcgttgcc
     2941 gcgtccggcc cgctgtcgaa tctggcgatg gctgttctgt ggggcgtggt tttggtgctg
     3001 actccgtatg tcggcggggc gtatcagatg ccgttggctc aaatggcaaa ctacggtatt
     3061 ctgatcaatg cgattctgtt cgcgctcaac atcatcccca tcctgccttg ggacggcggc
     3121 attttcatcg acaccttcct gtcggcgaaa tattcgcaag cgttccgcaa aatcgaacct
     3181 tatgggacgt ggattatcct gctgcttatg ctgaccgggg ttttgggtgc gtttattgca
     3241 ccgattgtgc ggctggtgat tgcgtttgtg cagatgttcg tctgactggc tttcagacgg
     3301 cataaacgct ccagaaaacg cggcaggaca tattgccctg ccgcgttttc ctgtagtgta
     3361 atcttatttt tttcatcatt attagaacca agttgcatga taataccttt cattaactga
     3421 aacactgatt aagaaactcc agtctgtcta atgatgaggt tttcacatcg ccaaaacttg
     3481 ccaatcaaat gctggattta ttgccgtctg agatttggtc aaatccaaag gcgacattct
     3541 tagaccctgt gtgtaaatca ggggtatttt tgcgtgaaat cgtcaaacgc ttggatgaag
     3601 gcttgaccaa tcaaatacca gataaacaaa ctcgcattaa ccacatttta aaaaatcaag
     3661 tttttggaag tactgccacg tatgtaggta gctttgaccg atatttgcat aaaaactcct
     3721 ttgctggtga aaggaattat tttgccaatt ttaaaatatt tctagcacca aatagtacaa
     3781 tgacaaagac aatcatgcca atgattaaat caggatagct agaatgagtc aataacgtca
     3841 atgctcccgc cgctatcaca ccgatattga tgataatgtc attggatgta aaaatcatgc
     3901 tggctttgat atggatttct ttattttgat ttttgctcag tagatataag cacagccagt
     3961 ttgcaatcaa tgccaaaaat gccgtgccaa tcatcagttg ataattgggc agctgctcag
     4021 caccgataaa acgcctaatc acttctatca ccccaaataa cgccaatatt atctgcgtta
     4081 tccccgccaa aaatgccaca cgttttttat acgccagcgt cataccaatg gctgatagcg
     4141 ccaatatata gacaaagctg tccgccagca tatctagact atcagcaatc agccccatag
     4201 aattagcaaa aataccaacc gaacactcta tgataaaaaa cacaaagtta atcatgagca
     4261 cttgatataa taatcttttt tctaagtgct catcaggctt gttaaacact atcttatcaa
     4321 caatcacttc ggtggaaatg atatgactat caaaattaag cggttcaagt acttgtaaaa
     4381 tcgttgtatc ttgattatcg tgatagacgg ttaagcaccg cccagcaata tcaaactgta
     4441 attcataaat atcagacaca tcttttaaac gcatgcgaat gagctgttct tcggacgggc
     4501 agtccatttt ggtaatgtta aaaatggtct ttttcatcta tttagttcct tgttttgatc
     4561 aggttggctc aaataaatct gtgtttatat tgctgcttgg taatttttgg atggtttgag
     4621 taaattgatt aggttaaaat ttacctttgg aagtaccgcc acgcataata gtttagatat
     4681 gtttataatc tctggataaa aaaacgtaat aagtgtttgc tggataacaa agtccaaacc
     4741 aatagcagat aaaataaggc atccaccccc tttcttcatt aaggataaga gatatcgaga
     4801 aacaaatcat aactaaacag aaaaaatttt ggagataaag ccatttcatt cccctattca
     4861 agaatctggc caagataggt attttgtata ctctagaaaa gaaaggcatt tccaagggaa
     4921 acatatcaga taaaaacttt tgtttatttt ttactataga tagaacccta tttctcaaga
     4981 taaatccaaa tgcaatgcca attatcatta ttaataaaca aaggatagta taactatgga
     5041 taaccttcat taaacaaaaa gaagaaatgg cagataaatt acatacgata tagtggaata
     5101 taaaatattt acggtctaaa ccttgttcag ttgcaatttt tttatactta ccttgcgcaa
     5161 aaaaatcaaa gaaatccatt aaactatctt tcacattaga aatttaaaag ctaaataata
     5221 caacaaacaa tgtgaagtac tattcatggt ttatttaaaa aataatacta ttctgaacat
     5281 tatttagata cagaaattaa caaattagaa ctaaacaagc ttttaaatac tttaatttta
     5341 ttggaaagct ataaaaggaa ctataacttt acacactagt cacttctttt taagaggcaa
     5401 aagggattgg gaaggtcgtc ttggagataa gcactggtat ttcggccaat ggtaaataga
     5461 gtttacctca aatagggtag aacctccttc atctgtcagt taataacagc cacttttaca
     5521 atgccctgtc aaaataaagc ggcacgcccg atttttcact catcatcatc aaataaccca
     5581 tcaccttttg aggccattcg ataccgcgca ccacggtcag attcctcaaa acggggaaaa
     5641 ccaaaatatc ctccatgccg attccgccgt tgatgccgtc tgaagcaccg tccatcaaat
     5701 tctccaactc ttgcaaatct gcgtttatgc gttcgagata ttgggcggtt ttattcaaat
     5761 tggcggaaaa gctgccgatg cttttctctt ttttgtctgt aaaatatttt accgcttccg
     5821 gcgttgcaaa ttcgggcagc ccgattttga tcacgcgcgg ctgcaccagt ttgtcgttgt
     5881 atccgcccac cttgtccagc cacgcccgta tctcggggcg gacttcgtct ttcagacggt
     5941 cttcgcggtc gaaatgccgc acaatgtcca aactctcgcc cataaacgaa ccgtcttctt
     6001 tttgcaggac gggcacttgt ttcgcaccga tcataccgat cggcgttgcc tcgtcgtcgt
     6061 ttgccagcac ggcttcttca acgtccgcgc caaacagccc ggcagccatc cgcgcacgca
     6121 cacaaaacgg gcaatggtcg taaatataca gtttcatcaa aatattcctc gtcaacctgt
     6181 cggtaccggc taccttaaca ccccgcgcca cccgaaacaa gtttatcttc ccgcctatgc
     6241 accgtaaata aataa
//
LOCUS       DAWFAD010000110         6118 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWFAD010000110 DAWFAD010000000
VERSION     DAWFAD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.110"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="WGO49_001577"
     CDS             complement(152..2080)
                     /gene="dnaK"
                     /locus_tag="WGO49_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ0306671.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDHIIAEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTSD
                     IDDVILVGGQSRMPKVQEAVRDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2288..2560)
                     /locus_tag="WGO49_001578"
     CDS             complement(2288..2560)
                     /locus_tag="WGO49_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306672.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2746..3432
                     /locus_tag="WGO49_001579"
     CDS             2746..3432
                     /locus_tag="WGO49_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0306673.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRNTKNLSVISVKGDSMEGVLNDGDSILVN
                     HSENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNHPADDVEII
                     GRVEWFGRTI"
     gene            3564..3902
                     /gene="erpA"
                     /locus_tag="WGO49_001580"
     CDS             3564..3902
                     /gene="erpA"
                     /locus_tag="WGO49_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ0306674.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3919..4065
                     /locus_tag="WGO49_001581"
     CDS             3919..4065
                     /locus_tag="WGO49_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306675.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4062..4526
                     /locus_tag="WGO49_001582"
     CDS             4062..4526
                     /locus_tag="WGO49_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0306676.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPKVDCGLIRECLRENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWEKRAELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     GDGEIPEYLELHSEREDDISAPSQAEPPPRNIKPLVFGLLIAFLGYLLGKIF"
     gene            4561..6072
                     /gene="ubiB"
                     /locus_tag="WGO49_001583"
     CDS             4561..6072
                     /gene="ubiB"
                     /locus_tag="WGO49_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ0306677.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPVASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAVWVERLFADGKRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1378 a         1359 c         1644 g         1737 t
ORIGIN      
        1 aaaaatttcc agagaaccga tacaacagtt ggaacttggg tttgggaata ttacggtaga
       61 tgaacttgga acctctgtta tgctatggtc ttttatctca attgaaaaaa gcgcgaatca
      121 aacggttcgc gcttttttca gacggtatta attatttttt gtcgtctttt acttcttcaa
      181 agtcggcatc tacgacatca tcgtctttct ttgcagaagc attggcttgt tcgctttcgc
      241 ctgcttgggc ttcagcttgt gcttgagcgt aaaccatttc ccccagtttt tggctggctg
      301 cgcccagcgc ctcggttttg gcatcgatag cggctttgtc gtcgccttta actgcttctt
      361 cggcttcttt cagcgcggct tcgatttttt ctttctcggc tgcgtcgagt ttgtcgccgt
      421 agtcggccaa agattttttc acagagtgaa tcagggcttc agcttggttg cgggaagcga
      481 ccaattcagt cagtttttta tcttcctcgg cattggcttc ggcatctttc accatgcgtt
      541 cgatttcttc ttcgctcaaa cctgaagaac cttggatggt gatgttggct gctttgccag
      601 tgcctttgtc tttggcggaa acgtgcagga tgccgttggc gtcgatgtcg aaggtgactt
      661 cgatttgcgg cataccgcgc ggtgcgggtg cgatgtcgcc caagttgaac tgacccaaag
      721 atttgttggc agaagcgcgt tcgcgttcgc cttgcagtac gtggatggtt actgcgcttt
      781 ggttgtcttc ggcggtagag aacacttgcg acgctttagt cgggatagtt gtgttctttt
      841 gaatcagctt ggtcatcacg ccgcccatgg tttcgatacc caaggacaga ggggttacgt
      901 ccagcagcaa tacgtcgctg cggccgccgc tcaatacttc gccttggatc gctgcgccta
      961 cggcaacggc ttcgtcaggg ttcacgtctt tgcgcggttc tttgccgaag aaatctctaa
     1021 cggcttcttg tactttcggc atacgggact gaccgccgac caaaattacg tcgtcgatgt
     1081 cgctggtgct caagccggca tctttcaatg cggtgcggca aggctcgata gagcgggcaa
     1141 tcaggtcttc aaccaggctt tcgaatttgg cgcgggtaat tttcatcgcc aagtgtttcg
     1201 ggccggttgc gtccatggtg atgtacggca ggttaatttc ggtttgctgg ccgctggaca
     1261 attcgatttt ggctttttcg gcagcttctt tcaggcgttg tagagccatc acgtcttgtt
     1321 tcaaatcaat gccttgttct tttttgaact cggcgatgat gtggtcgatg aggcgttggt
     1381 cgaagtcttc accgcccaag aaggtatcgc cgttggttgc caatacttcg aattgtttgt
     1441 cgccgtcgag gttggcgatt tcgatgatgg aaatatcgaa agtaccgccg cccaagtcat
     1501 atacggctac tttgcggtct ttgttgtcgc ctttgtccat accgaatgcc aaagcggctg
     1561 cggtcggctc gttgatgatg cgtttcacgt ccaaaccggc gatacggcct gcgtctttgg
     1621 tggcttgacg ttggctgtcg ttgaagtagg cagggacggt aatcacggct tcggttactt
     1681 tttcgcccaa gtaagcttcg gcggcttctt tcattttacg caggacttct gcggaaattt
     1741 gaggaggaga cagctctttg ccttgtgctt ttacccatgc gtcgccgttg ttggctttga
     1801 tgatttcgaa aggcatagat tcgatgtcgc gttggacttc tttgtcttca aatttgtggc
     1861 cgatcaaacg tttggcggcg taaatagtgt ttttggcgtt ggttaccgct tggcgtttgg
     1921 caggcgcacc gacgaggatt tcgccgccgt ccaaataagc gataacggac ggcgtggtgc
     1981 gtgcgccttc tgcgttttcg atcactttgg tttgaccgtt ttcggaaatg gccaaacaag
     2041 agttggttgt acctaagtcg ataccgatta cttttgccat gtggataatc ctatttgatt
     2101 ttgcttattt tgagaaatat gttggaacat tttgtcccga tgggctgtaa atagggcggg
     2161 cggcgggctg tttcaagcta cagcatggct ataagtatat aactttatga atatattggt
     2221 tttatatttg atttaataca tttggctcca atgcattcaa gcataatgtt tcaaatggca
     2281 ggcaggttta ttcatagacg atgccggcga gcatttcctg ttcgttcaag ttgccgtact
     2341 ctttttccca gtcgtgtgaa gactcgatga tgtcgcattc tttggaaagg gagacttgtt
     2401 ctgcatccat atctttggcg ttcagtatgt tgaattgttc gcatagggat gcggataaag
     2461 tgatgtcagg ctgtttggct tcagaacggt tttcttggaa ggcaaagcag aatgcggtaa
     2521 atgccgcagc atagataaga tatttgccgg ttttcttcat tttctatcct ttttctgtca
     2581 actcaggatt aaacctatgg aaaatcttaa aaattatgta ttgagtaaga aaaatcataa
     2641 tttaaattta gtttatcata gttgttccgt ttttgggata gctaaggtaa aatatatttc
     2701 atgtttactt tagatgattg aatgaagggg agcggaagga tatttatgga aacctttaaa
     2761 gacagactgg tttttttatg gaaaagcgaa gcgaagcagg caaaaatcgc atccgatatt
     2821 gaaatgacga ttgcgggctt cagcaggata tggaatgaag gcggtctgcc gaagtctgaa
     2881 acattaaaaa aaatcaagca gttgaagggg tgtagtatcg attggctgct gaccggggag
     2941 ggtaatccgt ttccggatga agccccaaaa aaatcccttg cttacgatac cttgggaaat
     3001 gaagtcgata cggacgagtt tgtctttgtg ccgagatatg atattcgggc ggctgcggga
     3061 tacgggcagt ttgtcgatca tgaggaaccg gtatttacaa tggcgttcag acggcattgg
     3121 attgagaatt atgttacccg caatacgaaa aacctgtctg taatttccgt caagggggat
     3181 tcgatggagg gggttttgaa tgacggcgat tcgattttgg tcaaccatag cgagaatacg
     3241 ccgagggacg gactgtatgt gttgcggatt aatgaaaatc tgctggttaa acgtttacag
     3301 attgtaccgg gcgggataat caacgttatt tccgccaacg aggcttaccc tgcttttgaa
     3361 attaatttga atcatcctgc cgatgatgtg gaaatcatcg ggcgggtcga gtggttcggc
     3421 aggacgattt gagtttgggg cttgaaatcg ggagcggtca aacttatctg taaggcagtt
     3481 ccttttcagg gaacaagccc ccgcttgcct ttgaaggggg tttgagagag aatgcagaaa
     3541 atattatatt aaggaataac accatgtcgg atgaaagccc tattattttt actgacagct
     3601 gctgtgccaa agttgccgat ttgattgccg aagaaaacaa tcccgatttg aaattgcggg
     3661 tttttgtcaa tggcggcggc tgttcgggtt tccagtacgg atttactttt gatgaaatca
     3721 aaaacgacga cgattttgaa attgagaaaa acggtttggt ctttttggtc gatccgatga
     3781 gctatcaata tctggtcggc gcggaaatcg actatacgga aagtttgcag ggttcgcaat
     3841 tcgtcatccg caatccgaat gcggaaacaa cctgcggttg cggatcgtcg ttttccgtat
     3901 gaccgcttgg tttgtgtgat gccgtctgaa cgttcagacg gcatttttac ttttggaaaa
     3961 tatattatcg ggatgaattc acatataatc cgattgtttg aagatgaatc gggtttcccg
     4021 aaaggaacgg gcggaacggt atcaggcgta tttgttccct tatgattgag atgagtaaag
     4081 attaccgaaa cgatttgtac gatgtatatg tttcctaccc gcccaaagtg gattgcgggc
     4141 ttatccggga gtgtctcagg gagaatctcg gcgaagaaaa ggcggaagga ttgattgagt
     4201 ccctcgattc caaaccgcaa gtgctggttg aggaaaaatg tacctgggaa aagcgggcag
     4261 agttgcacga ttatttcagc tatttgggtt tggatattat tactcggaga tatatggagt
     4321 tagaaacggt cgtgccgccg gaggaagggg agggcgaagg agaaggcggg gatggggaaa
     4381 tacccgaata tcttgaactt catagcgaac gggaagatga tatttccgca ccttcgcaag
     4441 ccgaaccgcc gccccgcaat atcaaaccgc tggttttcgg gctgctgata gcctttttgg
     4501 gctatctgct cggtaagatt ttttgattgt ccgataaatg ctgtattcgg gattttatat
     4561 atgaaatggt tgaaacgcct gacggttatt gtcgggactt tttaccgcta tcggctggca
     4621 ggtctgtgcg cttcgctgat gggtagcggt tggatatgcg ctctgctgaa aatgatgccg
     4681 cagtcgtcca aattgaaaaa cgaaccgcct gccgtccgtc tgcgccttgc cttggaaagc
     4741 ctggggccga ttttcatcaa gttcgggcag gttttgtcca cacgccctga tttgattccg
     4801 cacgattacg ccgtcgaact ggcaaagctg caagacaaag tgccgccttt tgacgcgcgg
     4861 ctttcgcgtg aacaaatcga aaaatcgttg ggtcagtcca tcgaaaagct gtatgcggaa
     4921 tttgaaaccg agcccgtcgc cagcgcgtcc atcgcccaag tacacaaagc ccgcctgcat
     4981 tcgggcgaac gagtggctgt caaagttttg cgccccaacc ttttgcccct tatcgaacag
     5041 gatttgtcgc tgatgcgttt cggcgcggtt tgggtggagc gtctgtttgc cgacggcaag
     5101 cgtctgaagc cgctcgaagt ggtggcggag ttcgacaaat acctgcacga cgagttggat
     5161 ttgatgcgcg aagccgccaa tgccggacag ctcgggcgca atttccaaaa cagcaatatg
     5221 ctgattgtgc ccaaggtgtt ttacgactac tgcaccagcg acgtgctgac aatcgaatgg
     5281 atggacggca cgccggttgc cgacatcgcc aaactcaaag cagacggcat cgatttgcac
     5341 aaactcgccg attacggcgt ggaaatcttt ttcacgcagg tcttccgcga cggctttttc
     5401 cacgcggata tgcaccccgg caatattttg gttgccgccg acaaccgcta catcgccctc
     5461 gatttcggca tcgtcggcac gctgaccgat tacgacaaac gttatctcgc catcaatttc
     5521 ctcgcctttt tcaaccgcga ttaccggcgc gtcgccaccg cccacatcga atcgggctgg
     5581 gtgcctgccg acacgcgtgc ggaagaattg gaagccgccg tccgcgccgt gtgcgaaccg
     5641 gtgttcaaca aaccgatttc gcagatttcc ttcggcttgg tgctgatgcg cctgtttgaa
     5701 gtcagccgcc gcttcaatgt cgaaatccaa ccgcagcttg tgctgctgca aaaaacgctg
     5761 ctcaacatcg aaggcttggg ccgccagctc gatcccgatt tggacttgtg gaaaaccgcc
     5821 aaaccgtttt tggtgaaatg gatgaacggg caggtcggcc ctaaagccct ttggcgcaac
     5881 ctcaaaaacg aagcccccga ctgggcgcaa atcatccctt cattgccgcg caaaatcagt
     5941 gcgttgattg atgaaaaccg ccagcaggaa atgcgtgatg cctatatcca tttggtcaaa
     6001 gtgcagcagc ggcaaagcct gtggctggct gtgattgcgg ttgttttgct gctgattttg
     6061 cttttgaaat aggctttgtc cgaatcatcg cccgactccg cccgtttata aggaaatc
//
LOCUS       DAWFAD010000111         5947 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWFAD010000111 DAWFAD010000000
VERSION     DAWFAD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.111"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(81..485)
                     /locus_tag="WGO49_001584"
                     /pseudo
     CDS             complement(81..485)
                     /locus_tag="WGO49_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(791..2050)
                     /gene="rho"
                     /locus_tag="WGO49_001585"
     CDS             complement(791..2050)
                     /gene="rho"
                     /locus_tag="WGO49_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ0306678.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDTINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTSNYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(2270..4654)
                     /gene="ppsA"
                     /locus_tag="WGO49_001586"
     CDS             complement(2270..4654)
                     /gene="ppsA"
                     /locus_tag="WGO49_001586"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ0306679.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMRH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYSGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGNVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            5066..5887
                     /locus_tag="WGO49_001587"
     CDS             5066..5887
                     /locus_tag="WGO49_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ0306680.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
BASE COUNT         1279 a         1377 c         1691 g         1600 t
ORIGIN      
        1 tctctttgag ctaaggcgag gcaccgccgt actggttttt gttaatccac tataaaaatt
       61 tatggggctg tcctagataa ctaggataaa ctcgatttta ctaattgttt taaaatggaa
      121 atttgaactt ttatctcact gttgttaaaa cgtcgttcgt acccctttaa atacagctca
      181 aaatgcgctt tgggaatgcc gtcaaacttg cgtaaatgac gttttgcccg gtttcaaaag
      241 ttcccaattc cattgatatg gttttgtcgt tcagcaaaat aactttcatc tgcttctact
      301 ttgccgtcaa acatttccaa atgcggactg ttttgataaa taagtaatcg taaacgatga
      361 aaataatagg ctgcggtatt tttattaacg cctactaact ctgctgctgt tcttgcagtt
      421 acacctgcga caaacagttc aatgagttta ttttgtttat accggcttag acgacttttt
      481 ctcatagggg caactctaac ttaatttgaa tttccctagt tatctaggac agccccaaat
      541 ttatacaaaa atgagcgcgg ttcggcgcaa ccttgaatca agttcccgca tcggttttca
      601 ttgccggtac ggatgcgttc aagccggttt tgcaaaggcc gcgctttcgg caagcggaca
      661 cggacactgc cgacggttgc gccgttaacg gggggaggga ggagctgcgc cgaccgtgtg
      721 aatgaaagtg ccgtctgaaa cccgattttc aggcttcaga cggcatttcg cattaatgcg
      781 ggcggcgcgt ttatttgccg cgcatcagtt caaagaaatc atcattgttt ttagaggctt
      841 tgatttttcc gattaaaaat tcggctgcct cgatttcgtc catcgggtgc aggaacttgc
      901 gtaagagcca catacgttgc aactggtcgt tcggcacaag cagctcttcg cggcgcgtgc
      961 cggatttgtt gatgttgatg gcggggaaga ggcgtttttc cgccatacgg cggtcaaggt
     1021 gcaattccat attgccggtg cctttgaatt cttcgtaaat cacatcgtcc atacggctgc
     1081 cggtttcaac caatgcggtg gcgatgatgg tcagcgaacc gccttcttcc acgttgcgcg
     1141 ccgcgccgaa gaaacgtttg gggcggtgca gtgcgttggc atcgacaccg ccggttagga
     1201 ttttgcccga ggtaggcacg acggtattgt aggcgcgggc aaggcgggta atcgaatcca
     1261 gcaggatgac cacgtctttt ttgtgttcta ccatacgctt ggctttttca agcaccattt
     1321 cggcaacttg gacgtggcgt tgcgccggtt cgtcaaaggt ggaggaaacc acttcgccgc
     1381 ggacggaacg gctcatttcg gttacttctt cgggacgctc gtcaatcaag aggacgatga
     1441 gttcgacttc gggatagttt gaggtaacgg cgtgggcaat gttttgcagc atcacggttt
     1501 taccgctttt gggcggggca accaagaggg cgcgctgacc tttgccgata ggggaaatca
     1561 ggtcgatggc acgtccggtc aggttttctt cggactttaa gtcgcgttcc agcttcaact
     1621 gttcggtcgg aaacagcggg gtcaggtttt caaacaggat tttatggcgg catacttccg
     1681 ggtggtcgcc gttgatggta tcaagcctga ccagggcaaa atagcgttcg ttgtcttttg
     1741 ggacgcgcac gcttccttcg atggtgtcgc ccgtatgcag gttgaagcgg cggatttggg
     1801 tgggcgagac atagatgtcg tcggggccgg caagatagga cgtgtccgcg ctgcggagga
     1861 agccgaagcc gtcgggcagg atttcaagcg tgccggagca ggtgaaaccc tcgccttttt
     1921 tcatcatctg gcggacgatg gcaaatacga gatcttgttt gcggaatcgg ttggcgtttt
     1981 cgatgccgtg ttcttccgcc aattctaaga gtttggaaat gtgcagggtt tgtaattcgg
     2041 agacgtgcat aataatgatg tattttgaag aggaaaaaga caggcagatg ccgtctgaaa
     2101 gaagaagctg accgttgccg gttgctcggg gaagggggaa ttgtaggcag tcggcgcgtg
     2161 ggtgtcaaat attatcgcgg acggggcatc ggcaggaaat gccgtctgag cggagctgct
     2221 tggaaaaaaa tacccccgcg cttttcaggc tcgggggtat gggcattgat tatttgttca
     2281 attcattcgc caaatatagc caagtttcga tgacggtatc cgggttcagg gaaacgcttt
     2341 caatgccttc ctcaaccagc catttggcga agtccggatg gtcggacggg ccttgaccgc
     2401 agatgccgac atatttgttc tgcttgcggc aggcggagat ggcaaggtgc agcatcactt
     2461 tgacggcagg gttgcgttcg tcaaacgatt cggataccaa gccgctgtcg cggtcgagac
     2521 cgagggtcag ttgggtcatg tcgttcgagc cgatggagaa gccgtcgaag tattgcagga
     2581 attgttccgc caataccgcg ttgctcggca gctcgcacat cataatcagg cgcaggccgt
     2641 ttttgccgcg ttccaagccg ttttctttca gggctttgac aacggcttcg gcttcgccca
     2701 aagtgcggac gaacggaatc atgatttcaa cgttggtcaa ccccatttca tcgcggacgc
     2761 gtttcaaggc tttgcattcc aaggcgaaac agtctttgaa gttgtcggcg acataacgcg
     2821 ccgcaccacg gaagcccaac atcgggtttt cttcatgcgg ttcgtatacg ttgccgccga
     2881 ccaggttggc gtattcgttg gatttgaagt cggacatacg gacgatggtt ttacgcggat
     2941 aaaccgatgc ggccaatgtc gccacgcctt cggcgatttt atcgacgtag aagtcgacag
     3001 gggacgcgta accggcgata cggcgggtaa tttccgcttt taattcgtcg tcttgtttgt
     3061 caaattccaa caaggctttg gggtggatac cgatttggcg gttgatgata aattccatac
     3121 gcgccaagcc gatgccttcg ctgggcaggt tggcgaagct gaatgcgagt tcgggattgc
     3181 cgacgttcat catgactttt acaggtgctt taggcatgtt gtccaaagca acatcggtaa
     3241 tttgtacgtc cagcaggccg gagtagatga agccggtatc gccttcggca caggatacgg
     3301 taacttcttg accgtttttc agcaattcgg ttgcattgcc gcagccgaca acggcaggaa
     3361 tacccagttc gcgcgcgatg atggcggcgt ggcaggtacg tccgcccctg ttggtcacga
     3421 tggcggaagc gcgtttcatc accggttccc aatctgggtc ggtcatgtcg gtaaccagta
     3481 cgtcgccggc ttcgacggaa tccatctcgg aagcatcttt aatcaggcgt accttgccct
     3541 gaccgacttt ctgaccgatg gcgcggcctt cgcacaagac ggttttttcg ccgttgatag
     3601 aaaagcggcg caggttgcgg ctgccttctt cctgggattt gacggtttcg ggacgggctt
     3661 gcaggatgta gagtttgccg tccaagccgt cgcgtcccca ttcgatgtcc atcgggcggc
     3721 cgtagtgttt ttcgatggtc agtgcgtaat gcgccaactc ggtgatttct tcgtcggtaa
     3781 tggagaagcg gttgcggtct tcttcgggga catcgacgtt ggttaccgat ttgccggctt
     3841 ctgctttgtc ggtaaaaatc attttgatgt gttttgagcc catggttttg cgcaggatgg
     3901 caggtttgcc tgctttcagc gtgggtttga acacatagaa ttcgtcggga ttgactgcgc
     3961 cttgtacgac gttttcgccc agaccgtagg atgaagtgac aaagacgact tggtcgtaac
     4021 cggattcggt atcgagggtg aacatcacgc cggacgcgcc gctgtcggaa cgcaccatac
     4081 gctgcacgcc ggcggaaagg gcgacgatgt cgtgttcgaa gcctttgtgg acgcggtaag
     4141 aaatggcgcg gtcgttgtac agggaggcga atacatggcg catcgcttct ttaacgtttt
     4201 ccaagccgtt gatattcagg aaggtttcct gttggccggc aaatgatgcg tcgggcaggt
     4261 cttcggcggt tgcggaagag cgtacggcaa cggaaatgtc cgcgccgccg gcatcggcaa
     4321 ccattttgtt ccatgccgct tcgatttcgg catcgagctg ttcggggaag ggcgtgtcca
     4381 aaatccattg gcggatttct ttgccgacgc gtgccagttc ggcaacgtct tcgacatcta
     4441 attttgccag tgcggcggaa atgcgttcgc tcagaccgtt gtgtgcgagg aatgcgcggt
     4501 aggcatcggc tgtggtggca aagccgccgg ggacgcgaac gcctttttcg gtcagctgac
     4561 tgatcatttc gcccagcgag gcgtttttac cgcccacgcg ttcaacatct gtcatacgca
     4621 ggttttcaaa ccaaattacg tagttgtcgg ccatttgtgt gtccaatcca aaatatgtta
     4681 aaaaagaaac aaatccgcgt gcttatttta agcgattcgt tcctctgctg tcatgtgttt
     4741 tatccgtttt aaaatcatga tgccgtctga aaaattgcgg tttcggcgtg tgtagcggtt
     4801 tgaaacttgc agccggtata cttctttttt tgggtatttt ctttgtaaaa caggtggttt
     4861 gaataggtta atgttttttc tgtttgattt ttttgtttat tttttaaaat tttctgccaa
     4921 aaaatacttt atataaataa ttttatttca aaattatatt gtgtctgttt gggtgtaatc
     4981 cgaggtaggt gtgctgcgag tgctttcctt gtgtctgccg ctgctgttat gatgggattt
     5041 taaacctgtg ttttaaggat ggaagatgag cagtccgcgc catgtgtttt acatttccga
     5101 ccgtaccggt ctgactgccg agaatatcgg cgaggctttg ctgaaccagt ttggcaatct
     5161 gtcgttcaaa cgccatacgc atccgtttgt cgatacgccg gaaaaggcgc gcgcggtggt
     5221 ggagaaggtc aatcggagcc ggcaggaaaa cggtcagcgt ccgattgcgt ttgtcagtgt
     5281 ggttgatgac gaaatccgtc ggattatcaa aggggcggat gcttttcaga ttaatttctt
     5341 tgagactttt ttgggactgt tggagaaaga actcaatacc gaagccacgg catccgagca
     5401 ggggcatcac agtatcggta atacgaagcg ttacgatgcg cgtatggagg cggtgaattt
     5461 ctcattgaac cacgatgacg gggtcagcga taagaacctt caggaagcgg atgtaatctt
     5521 gatgggtgta tcgcgttcgg gcaaaacgcc gacctgcctt tacctcgccc tgcaatacgg
     5581 catccgtgcg gcaaactatc cgctgattcc cgacgatttg gaatcggccg atctgccgcg
     5641 tatggtcaag ccttataggg ataagctgtt cgggttgacc atccagccgg aacgtttgca
     5701 ggccatccgc caagagcgcc gcccgaattc aacttatgcc aaaatcgata catgccgcag
     5761 cgaggtggcg gacgcgcaga gtatgttcag acggcatggg attccgtttg cgaatacgac
     5821 ggataaatca gtggaagagc ttgccgtaca catccttcag gcgtgcaagc tcaaacgcag
     5881 gttttgacgg gttttgattc ggtttgaagg tggaactgcc gtctgaaatc aggtttcaga
     5941 cggcagt
//
LOCUS       DAWFAD010000112         5929 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWFAD010000112 DAWFAD010000000
VERSION     DAWFAD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.112"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            55..1593
                     /gene="murJ"
                     /locus_tag="WGO49_001588"
     CDS             55..1593
                     /gene="murJ"
                     /locus_tag="WGO49_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ0306681.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLASGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLWAAQACLPFEWVRAGGMRKAGQLCILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            1726..2682
                     /locus_tag="WGO49_001589"
     CDS             1726..2682
                     /locus_tag="WGO49_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEZ0306682.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSGSHYAVELMLAVRDRGWNGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGERGENALPQAAA
                     VISAPVDAEAAGRRFDSGITRLLYTRYFLRTLIPKARSLHGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHAGFVGSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            2733..3515
                     /gene="trpC"
                     /locus_tag="WGO49_001590"
     CDS             2733..3515
                     /gene="trpC"
                     /locus_tag="WGO49_001590"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ0306683.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMQSRGVHTFLIGETFMRADNIEAEVGKLF"
     gene            3581..5881
                     /gene="recQ"
                     /locus_tag="WGO49_001591"
     CDS             3581..5881
                     /gene="recQ"
                     /locus_tag="WGO49_001591"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ0306684.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSGTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGM
                     LAERYPNVPRIALTATADAATRADIKHYLHLDDAPEFISSFDRPNIYYQVIEKNNGKK
                     QLLEFIRKEMAGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGAELSDKEWRSVIRQCISLGYLTVNIARYQALQLTEAAKKVLKGETEVMLRPLK
                     RGKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEGIPAYMIFGDKTLRDLVEKMP
                     QNLNGLHDIYGLGEAKTERFGHGILEVCRNAAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDETAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         1293 a         1762 c         1618 g         1256 t
ORIGIN      
        1 aaagtctgat cctgccgccc taaagggcgg ggtttcaacc gaaaaggaaa tacgatgaat
       61 atgctgggag ctttggcaaa agtcggcagc ctgacgatgg tgtcgcgcgt tttgggattt
      121 gtgcgcgata cggtcattgc gcgcgcattc ggcgcgggca tggcgacgga tgcgtttttt
      181 gtcgcgttca aactgcccaa cctgcttcgc cgcgtgtttg cggagggggc gtttgcccaa
      241 gcgtttgtgc cgattttggc ggaatataag gaaacgcgtt ctaaagaggc gacggaggct
      301 tttatccgcc atgtggcggg gatgctgtcg tttgtgctgg tcatcgttac cgcgctgggc
      361 atacttgccg cgccttgggt gatttatgtt tccgcacccg gttttgccaa agatgccgac
      421 aaatttcagc tctccatcga tttgctgcgg attacgttcc cctatatctt attgatttca
      481 ctttcctctt ttgtcggctc ggtactcaat tcctatcata aattcagcat tccggcgttt
      541 acgcccacgt ttctgaacgt gtcgtttatc gtattcgcgc tgtttttcgt gccatatttc
      601 gatcctcccg ttaccgcact ggcatgggcg gtttttgtcg gcggcatttt acaactcggc
      661 ttccaactgc cctggctggc gaaactgggt tttttgaaac tgcccaaact gaatttcaaa
      721 gatgcggcgg tcaaccgcgt catgaaacaa atggcacctg cgattttggg cgtgagcgtg
      781 gcgcagattt ctttggtgat caacacgatt ttcgcgtctt atctgcaatc gggcagcgtt
      841 tcatggatgt attacgccga ccgcatgatg gagctgccca gcggcgtgct gggggcggca
      901 ctcggtacga ttttgctgcc gactttgtcc aaacactcgg caaaccaaga tacggaacag
      961 ttttccgccc tgctcgactg gggtttgcgc ctgtgtatgc tgctgacgct gccggcggcg
     1021 gtcggactgg cggtgttgtc gttcccgctg gtggcaacct tgttcatgta ccgcgaattc
     1081 acgctgtttg acgcgcagat gacgcaacat gcgctgattg cctattcttt cggtttaatc
     1141 ggtttgatca tgattaaagt gttggcatcc ggcttttatg cgcggcaaaa catcaaaacg
     1201 cccgtcaaaa tcgccatctt cacgctcatc tgcacgcagt tgatgaacct tgcctttatc
     1261 ggcccactga aacacgtcgg actttcgctt gccatcggtc tgggcgcgtg tatcaatgcc
     1321 ggattgttgt tttacctgtt gcgcagacac ggtatttacc aacctggcaa gggttgggcg
     1381 gcgttcttag caaaaatgct gctctcgctc gccgtaatgg gcggcggact gtgggcggcg
     1441 caggcttgcc tgccgtttga atgggtacgc gccggcggaa tgcggaaagc ggggcagctc
     1501 tgcatcctga ttgccgtcgg cggcggactg tatttcgcat ctctggcggc tttgggcttc
     1561 cgtccgcgcc atttcaaacg ggtggaaagc tgaccgatgc cgatatcctt tttcggaaaa
     1621 cgctttttcc gcgcctatat tcgggcgcgc atcccccacg tccgcaccag ccggacgggc
     1681 tgccgcatca tcaatgccgt ctgaaaacaa gaaaaccgat accttatgat tttaacaccg
     1741 ccggacacgc cctttttcct ccgcaacggc aatgccgaca cgattgccgc caaattcctg
     1801 caacgccccg cacctgcata ccgccgcgag ctgcttccag acagcacggg taaaaccaaa
     1861 gtcgcctacg acttttcaga cggcatttcg cccgacgcac cgctggtcgt gctgtttcac
     1921 ggtttggaag gaagcagtgg cagccattac gcggtcgaac tgatgctcgc ggtacgcgat
     1981 cgcggttgga acggcgcagt cgtccatttc cgcagctgcg gcggcattgc caacaccgct
     2041 ccggtgttct accacttggg cgataccgcc gaaatcgcct ttactttgga cacgcttgcc
     2101 gcgcgttacc gtgaaatata cgccgtcggc gtatcgctgg gcggcaacgc gctggcaaaa
     2161 tatttgggcg aacggggcga aaacgcgctg ccgcaagccg ctgccgtcat ctccgcaccc
     2221 gtcgatgcag aggcggcagg cagacgcttc gacagcggca tcacgcggct gctctacacg
     2281 cgctacttcc tccgcaccct gatacccaaa gcacggtcgc tccacggttt tcagacggca
     2341 tttgccgcag ggtgcaaaac actgggcgag tttgacgacc gcttcaccgc accgctgcac
     2401 ggctttgccg accggcacga ctactaccgc caaacttcct gcaaaccgct gctcaaacac
     2461 gttgccaaac cgctgctcct gctcaatgcc gccaacgacc ccttcctgcc gcccgaagcg
     2521 ctgccgacac cggacgaagt gtccgaagcc gttaccctgt tccagccggc acacggcgga
     2581 cacgccggct ttgtcggcag caccggcggc aggctgcacc tgcaatggct gccgcagacc
     2641 gtcctgtcct atttcgacag cttccgcaca aacaggcggt aacggtttga tgctaatatt
     2701 ccccctttcc ccggacaaat acggaacacg acatgaccga catcctcaat aaaatcctta
     2761 ccgccaaggc acaggaagtt gccgctcaaa aagccgccgt caacgccgaa cacatccgcg
     2821 cacttgccgc agaagccgcg cccgtccgca gcttcatcgg ttcgatacgc ggcaaacacc
     2881 gcctaaacct gcccgccgtc attgccgaaa tcaaaaaggc aagcccgagc aaaggcctga
     2941 tccgtccgga cttccgaccg gccgagattg cacgcgccta tgaaaacgcc ggggcggcgt
     3001 gtttgtccgt actgaccgac gaaccctatt tccaaggttc gcccgaatac ctcaaacagg
     3061 cgcgcgaagc cgtatcgctg cccgtgctgc gcaaagactt catcatcgac gaatatcagg
     3121 tttatcaggc gcgcgcatgg ggggcggatg ccgtcctgct gattgccgca gcactggaac
     3181 aggaacaatt ggaacgcttt gaagcggtgg cgcacgaatt gggcatgacc gtcctgctcg
     3241 agctgcacga tgaaagcgaa ttggaaaaat gccgcaacct gaccacgccg ttgtggggtg
     3301 taaacaaccg caacctgagg acttttgaag tctccctcga ccaaaccctg tcgctgctgc
     3361 ccgcgctgga aggcaaaacc gtcgttaccg aaagcggcat tacaggcaag gcggatgtgg
     3421 aatttatgca atcgcgcggc gtgcatacct tcctgatcgg cgaaacgttt atgcgtgccg
     3481 acaatattga ggcggaagta ggcaaactct tctaaatccc gatttcagac ggcattccct
     3541 ttctttaacc gacacaccct ttaccggaca cggaaccatc atgcaccgac cgaccgccaa
     3601 acagattctg cacgaagtat ttggttatcc cgaatttcgc ggcaggcagg aggctgtcat
     3661 caatactttg gcaggcggcg ggagtttgac ggtgctgatg ccgacgggcg ggggcaagtc
     3721 tttgtgttac cagattcccg cgctgatgcg cgaaggcgtg gcggttgtcg tatcgccgct
     3781 gattgcgctg atgaacgacc aagtggccag cctgcatgtg gccgggattg aagcagcggc
     3841 agtcaacagc ggcacatcgg cagatgaggc gcgcgagatt gccgacaagc ttgcccaagg
     3901 ccgtctgaaa ctgctttatg tcgcgccgga acgcttggtt accgatcgct ttttgcgttt
     3961 tctcgaccaa caaaccgtca gcctgtttgc cattgatgag gcgcattgcg tcagccagtg
     4021 gggacacgat ttccgccccg aataccaaca gctcggcatg cttgccgaac gctatccgaa
     4081 cgtcccgcgc atcgccctga ccgccaccgc cgatgccgcc acgcgcgccg acatcaagca
     4141 ttatctgcac ttggacgatg cgcccgaatt tatttccagc ttcgaccgcc cgaatattta
     4201 ttatcaggtt atcgaaaaaa acaacggcaa aaaacaactg cttgaattta tccgcaaaga
     4261 aatggcgggg caaagcggca ttgtgtattg cctaagccgc aaaaaggttg aagatgtggc
     4321 gcagtttttg cgtgaaaacg gattaaacgc gattccgtat catgctggtt tgagcatgga
     4381 cgtgcgcgag gaaaaccaac gccgctttac gcatgaagac aatattatcg tggtggcgac
     4441 cgtggcgttc ggcatgggca tagacaaacc cgacgtgcgc tttgtcgccc atctcgatat
     4501 gccccagagt gtcgaacatt tctatcagga atcggggcgt gccggcaggg acgggctgcc
     4561 tgccgcgagc tggctgtgtt acggcttgaa cgattgggtg ttgctgcgcg aacggattgc
     4621 cgaaggcaac agcgatgagg tgcaaaagca aatcgaaatg caaaaactcg atgccatgct
     4681 ttccgtctgc gaaaccgccg cctgccgccg cgtactgttg ctcaaacatt ttggcgaaga
     4741 atccgaaccc tgcggccatt gcgacaactg cctgcatccg cctgtgcggt ttgacggcac
     4801 ggtgttggta caaaaattac tcagctgcgt gtaccgcgcc ggacaacgtt ttgccgccgg
     4861 ttacatcacc aaccttttgc gcggtaaaag cgacgattgg atacgcggca accggcacga
     4921 acaactgtct accttcggca tcggtgcgga gttgtccgac aaagaatggc gcagcgtcat
     4981 ccgccagtgc atcagcctcg gctacctcac cgtcaacatt gcccgatatc aggcattgca
     5041 actgaccgaa gccgccaaaa aagtcctcaa aggcgaaacc gaagtgatgc tgcgtccgct
     5101 caagcgcggc aagcccgccg cccgcaccct caaagacaac tggctgcgta ccgaacgcga
     5161 agaacgcctg tggcaggcat tgcgcgtttg gcgtatgaaa caggcagaag ccgaaggcat
     5221 ccccgcctat atgattttcg gcgacaaaac cctgcgcgac cttgtcgaaa aaatgccgca
     5281 aaacctcaac gggctgcacg acatctacgg cttgggcgaa gccaaaaccg aacgtttcgg
     5341 acacggcata ctcgaagtct gccgcaacgc cgccggcttc agccgcgacg cggtcatccg
     5401 tccgcaaacc gaacgcgaac aacaactgcg tcaaaaactc gaggcctggc ggtatgaaca
     5461 ggcaagggcg gaaaactgcg ccctgcatgc cgtcctctcc gacgaaagcc ttgccgatat
     5521 gcttgccgat acgcccgaaa ccgaaaccga cctcgaaggc gtacacggct tgggcagcgt
     5581 acgcgccgcc aaatacggac gggacatcct cgccgtctgc cgtccgtttt cagacggcat
     5641 cgatgaaacc gccaaacaca aacgccgcct gatgcgcgcc ctgatccaat ggtgcaacga
     5701 aacggcaaaa cacgaacagt ccgaacccta ccgcattctc agcaaagccg ccctacgcgc
     5761 cattgccgcc aaacagccgg aaggtttggc ggagcttgcc gccgtatatg gcgtaggcga
     5821 agaaaaagcc gcacgttacg gtgcggcggt gttggcggta ttggaacgaa atgccgtctg
     5881 aaaaccgtta ccaagtttca gacggcattg cctctattta aaaattcct
//
LOCUS       DAWFAD010000113         5827 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWFAD010000113 DAWFAD010000000
VERSION     DAWFAD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.113"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1040..3766)
                     /locus_tag="WGO49_001592"
     CDS             complement(1040..3766)
                     /locus_tag="WGO49_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HEZ0306685.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAEQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKSSDTLSKEQVLNIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGVSQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSS
                     EYGNGALAGSVAFQTKTAADIIGEGKQWGIQSKTAYSGKDHALTQSLALAGRSGGAEA
                     LLIYTKRRGREIHAHKDAGKGVQSFNRLVLDEDKKEGGSQYRYFIVEEECHNGYAACK
                     NKLKEDASVKDERKTVSTQDYTGSNRLLANPLEYGSQSWLFRPGWHLDNRHYVGAVLE
                     RTQQTFDTRDMTVPAYFTSEDYVPGSLKGLGKYSGDNKAERLFVQGEGSTLQGIGYGT
                     GVFYDERHTKNRYGVEYVYHNADKDTWADYARLSYDRQGIDLDNRLQQTHCSHDGSDK
                     NCRPDGNKPYSFYKSDRMIYEESRNLFQAVFKKAFDTAKIRHNLSINLGYDRFKSQLS
                     HSDYYLQNAVQAYDLITPKKPPFPNGSKDNPYRVSIGKTTVNTSPICRFGNNTYTDCT
                     PRNIGGNGYYAAVQDNVRLGRWADVGAGIRYDYRSTHSEDKSVSTGTHRNLSWNAGVV
                     LKPFTWMDLTYRASTGFRLPSFAEMYGWRAGESLKTLDLKPEKSFNREAGIVFKGDFG
                     NLEASYFNNAYRDLIAFGYETRTQNGQTSASGDPGYRNAQNARIAGINILGKIDWHGV
                     WGGLPDGLYSTLAYNRIKVKDADIRADRTFVTSYLFDAVQPSRYVLGLGYDHPDGIWG
                     INTMFTYSKAKSVDELLGSQALLNGNANAKKAASRRTRPWYVTDVSGYYNIKKHLTLR
                     AGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMKF"
     gene            complement(3853..5652)
                     /locus_tag="WGO49_001593"
     CDS             complement(3853..5652)
                     /locus_tag="WGO49_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0306686.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVETVQDMHSKPK
                     YEDEKSQPESQQDVSENSGAAYGFAVKLPRRNAHFNPKYKEKHKPLGSMDWKKLQRGE
                     PNSFSERDELEKKRGSSELIESKWEDGQSRVVGYTNFTYVRSGYVYLNKNNIDIKNNI
                     VLFGPDGYLYYKGKEPSKELPSEKITYKGTWDYVTDAMEKQRFEGLGSAAGGDKSGAL
                     SALEEGVLRNQAEASSGHTDFGMTSEFEVDFSDKTIKGTLYRNNRITQNNSENKQIKT
                     TRYTIQATLHGNRFKGKALAADKGATNGSHPFISDSDSLEGGFYGPKGEELAGKFLSN
                     DNKVAAVFGAKQKDKKDGENAAGPATETVIDAYRITGEEFKKEQIDSFGDVKKLLVDG
                     VELSLLPSEGNKAAFQHEIEQNGVKATVCCSNLDYMSFGKLSKENKDDMFLQGVRTPV
                     SDVAARTEANAKYRGTWYGYIANGTSWSGEASNQEGGNRAEFDVDFSTKKISGTLTAK
                     DRTSPAFTITAMIKDNGFSGVAKTGENGFALDPQNTGNSHYTHIEATVSGGFYGKNAI
                     EMGGSFSFPGNAPEGKQEKASVVFGAKRQQLVQ"
BASE COUNT         1377 a         1488 c         1287 g         1675 t
ORIGIN      
        1 gttcccacgg tcaggatttc agattgcgga catctgtcag aaaagacaaa aaaccttccg
       61 ccgtcattcc ctacaggcga gaatccgatc cgttgaaatt cggttgtttt aaataaattc
      121 ttacagcttt gattttctgt ttttccgata acgccgtaac tttgaaacgc gaaagcggta
      181 atccgatccg ttgggatttt gcaacttcaa atcaatccgc aaactgaaat cccgtcattc
      241 ccgcgcagtc gtgaatccga acgcgtccgc acgaaaacct gcatcccgtc attcccacgg
      301 aagtgggaat ctaggacgta aaatctcaag aaaccgtttt atccgataag tttccgcacc
      361 gacagacctg gattcccgcc tgcgtgggaa tgacgaaatt tcagcgagcc gtagggtggg
      421 ctgtagggtg ggcttcagcc caccaatttc accgcatcaa aggtttttgg gaatacggcg
      481 gtttcggttt tcggtgggaa tgggcggaat ggataacatt gacggagttg ggggaatagg
      541 tggaaacggt gggattggtg ggctgaagcc caccctacag ccagccctat gtccacgctg
      601 cacatcctac gcccgccttg cgtttcttat aagcagcatt ttacgcaata ctttgcgtcc
      661 gctgaaaaaa tttcaaacgg tcttttttat caaatgcgaa aaatataacg tcattcccgc
      721 gcaggcggga atctagtctg tcggtgcgga aacttatcgg ataaaacggt ttctttagat
      781 tttacgccct agattcccgc ctgcgcggga atgacgggat ttgaggtttc tgtttttgat
      841 tttccgcctt cgcgggaatg acggtttaga agttacccga aacctcaaaa aaaaccgaaa
      901 ccgaacggtc tggattcccg cctgcgcggg aatgacgggt ttcgagatta cggtgtatcg
      961 gggatgatgg gaaacggtgg gaattgtgta aaaaatgccg tctgaagcct ttcagacggc
     1021 atttgcggcg tttggacgtt taaaacttca tttccaagct aaatgtgtag tttcggccgg
     1081 gggcggcata tcggttgtaa acgccgacat ttttgtgttg gttgactgcg ccgccggcag
     1141 tttgccgcac attttcccaa gtaacatagc ggtagttgag gaggttgtac acacctgcgc
     1201 gcagggtcag gtgtttcttg atattgtaat atccggaaac atccgtaaca taccaaggcc
     1261 gcgtccgccg tgatgctgct tttttagcat tggcattacc gttcaacagc gcctggctgc
     1321 cgagcagttc gtcaacagat tttgccttgg aataagtaaa catcgtattg atgccccata
     1381 ttccgtcagg atggtcgtaa cccaaaccca atacatatcg tgaaggttgg acggcatcaa
     1441 agagatatga agttacaaac gtcctgtcgg cgcgtatatc ggcatctttg accttgatac
     1501 ggttataggc aagcgtggaa tacaacccgt ccggcaaccc gccccatacg ccgtgccaat
     1561 cgattttacc caaaatattg ataccggcta tccgtgcatt ttgggcattt cggtatccgg
     1621 ggtcgccaga agccgaagtt tgcccgtttt gagttcgggt ttcataaccg aatgcaatca
     1681 ggtcgcgata ggcattgttg aaatagctgg cttccaaatt gccgaagtcc cctttaaata
     1741 caatacctgc ctctctatta aaggattttt ccggtttcag atccaacgtt ttcaaagact
     1801 ccccggctct ccagccatac atttcggcaa acgacggcag acggaagccc gtagaagcgc
     1861 gataagtcaa atccatccag gtgaaaggtt tgaggactac gcccgcgttc caagaaaggt
     1921 tgcggtgagt gccggtagag acactcttat cttccgaatg cgtgctgcgg taatcgtaac
     1981 gtatgcctgc tccgacatcc gcccacctgc ccaaacggac attgtcttga acggctgcat
     2041 aataaccgtt gccgccgata ttcctcggtg tgcagtctgt ataggtgtta ttgccgaaac
     2101 ggcatatcgg cgatgtattg accgtggtct tgccgataga caccctatac gggttgtctt
     2161 tgcttccgtt gggaaacgga ggctttttcg gggttatcaa atcatatgcc tgaactgcgt
     2221 tttgaagata ataatcgctg tgggacaatt gcgacttaaa gcggtcgtac cctagattga
     2281 tactcaaatt gtgacggatt ttggccgtat caaatgcctt tttaaatact gcttggaaca
     2341 ggtttcggct ttcttcataa atcatccggt cggatttata gaaagaatac ggtttattgc
     2401 cgtcgggacg gcaattttta tccgaaccgt cgtgagagca atgcgtctgc tgcaaacggt
     2461 tgtccaaatc tataccttgc cggtcataag aaagtcgggc gtaatcggcc caggtatcct
     2521 tatcagcatt atggtaaaca tattcgaccc cgtagcggtt tttagtatgg cgttcatcat
     2581 aaaacacgcc ggtaccgtaa ccgataccct gcaatgtact gccctctccc tgaacaaaca
     2641 gcctttctgc cttattatcg cccgaatatt tgccaagacc tttcagcgaa ccgggtacat
     2701 aatcttcact ggtaaaatag gcaggaacag tcatatcccg tgtatcaaag gtctgctgcg
     2761 tacgttcgag aacggctccg acataatggc ggttgtccaa atgccaaccc ggtcggaaca
     2821 gccatgattg gctgccatac tcaagcgggt tcgcaagtaa gcggttggag ccggtataat
     2881 cctgcgtgct gacggttttg cgctcatctt tgaccgaggc atcttctttc agcttgtttt
     2941 tacaggccgc atatccattg tggcattctt cttcgacaat gaaatatctg tactgactgc
     3001 caccctcctt cttgtcctcg tccaacacca gccggttgaa gctctgcaca cccttgccgg
     3061 catctttatg cgcatggatt tcccgacccc gccgtttagt ataaataagg agggcttccg
     3121 cgccgccgct gcgtccggca agcgcaaggg attgcgtcag ggcatggtct tttcccgaat
     3181 aggcagtttt actctgaatg ccccactgtt ttccctctcc gataatgtcg gctgcggttt
     3241 tggtttgaaa tgcgaccgaa cctgccaatg cgccgtttcc gtattctgat gaattcgaac
     3301 ccttgctgat ttcaacggcc ttgacgtttt catactcgat ttcattgatt gcgccgctgc
     3361 tacccgccgt cctcgtccca cccaatgccg cctgcgcggt gtaggactgt atttgcgaaa
     3421 cgccgtctac cgttaaggaa acgcggtttt tatccatgcc gcgtattgaa tagccggaac
     3481 ttgcgccccg accctgttcg accacggcaa tacccggatc ataacgggtc aggtctcgga
     3541 tattcaaaac ctgttcttta cttagcgtat cggaagactt gaccaacttg cccagcccgg
     3601 ttacttcgtt atcgcggcgg gttttctgtt ttttggcttt tacctgtatg gtatccaact
     3661 gtttttcctg tgcttgttcg gcttgcacat tttctgcata aacgggcagc gcggtcatta
     3721 aagacaggca taaaatattt aatcggaaca aatgttgctg ttgcatagtg tttccctaat
     3781 cttcgctttc agacggcatc ggaaggaacg gtgccgtctg aagccttatt ctcgattgtt
     3841 cggcagccgt gcttattgca caagctgttg gcgtttcgca ccgaatacca ccgatgcttt
     3901 ttcttgtttt ccctctggtg catttcccgg aaatgagaac gatccgccca tctcgatggc
     3961 gtttttgccg tagaaaccgc cggatacagt ggcttcaata tgcgtatagt gggaatttcc
     4021 ggtattttgc ggatccagcg caaagccgtt ttcaccggtt ttcgccacac ctgaaaaacc
     4081 gttgtcctta atcatggcag taatagtaaa cgcaggagac gtacggtctt ttgccgtcag
     4141 tgtgccactg atttttttag tggaaaaatc cacgtcaaac tctgccctat taccaccttc
     4201 ctgattggag gcttcgccgc tccagcttgt gccgttggca atatatccgt accaagtacc
     4261 gcgatatttg gcgtttgcct ccgtccttgc cgccacatcg gatactggag tgcggacacc
     4321 ttgcaggaac atatcgtctt tattttcttt tgacagcttc ccaaaactca tgtaatccaa
     4381 gttggaacaa cacaccgttg ccttcacgcc gttttgctca atctcgtgct gaaatgccgc
     4441 cttattgccc tcagacggca gcagtgaaag ctccactccg tcaaccagca gctttttcac
     4501 atctccaaaa ctgtctattt gctctttctt aaactcctcg ccggtaatac ggtatgcatc
     4561 tatcacggtt tccgttgcag gccctgccgc gttttcccca tccttcttat ctttctgctt
     4621 cgcaccaaac accgctgcaa ctttgttgtc gttgctcaag aatttaccgg caagttcctc
     4681 gcctttcggc ccgtaaaatc cgccttccaa actgtcggag tcggaaataa agggatgact
     4741 tccatttgtt gcacctttat ctgccgccaa cgccttacct ttgaaacggt tgccgtgaag
     4801 agttgcttga atggtgtaac gcgtagtttt tatttgtttg ttttcactat tattttgagt
     4861 aatacggttg ttacgataaa gtgtgccctt tattgtttta tcagaaaaat caacctcaaa
     4921 ctcactagtc ataccaaaat cggtatgacc ggatgatgcc tctgcctgat tacgcaatac
     4981 cccttcttct aatgcagaca acgcccccga tttatctcct cctgctgcac tacccaatcc
     5041 ttcaaacctt tgtttttcca tagcatcagt aacataatcc caagtacctt tataagttat
     5101 cttttccgat ggcagctcct tggaaggttc tttcccttta tagtaaagat atccgtcagg
     5161 tccaaaaaga actatattat tcttaatatc aatattattt ttattaaggt aaacatatcc
     5221 cgaacggaca taagtgaaat ttgtataacc aactacacga ctttgcccat cttcccattt
     5281 tgattcaata agttcagaac taccccgttt tttttccaat tcatccctct cactaaaact
     5341 atttggttct cctctttgca gttttttcca atccattgaa cccaatggtt tgtgcttttc
     5401 cttatattta ggattaaaat gtgcattccg gcgaggtagt tttactgcaa agccataagc
     5461 cgcgccgctg ttttccgata catcctgttg gctttcaggc tggctttttt catcctcata
     5521 cttaggtttg gagtgcatat cttgcacggt ttccacgctg tccaaatcga aactgccgcc
     5581 gccacccaga caagcactca acaaaaacac aggcagcacc atagcagcct gatttaccaa
     5641 tggattgttc atataaaccc aattcaatta aagaatgata aggattatta ttttatttat
     5701 ttttaacaaa tttgcaaata cttttttatt tttttaggga atacaccaaa atcccgtcat
     5761 tcccgcgcag tcgtgaatcc gaacgcgtcc gcacggaaac ctatatcccg tcattcccac
     5821 gtatatc
//
LOCUS       DAWFAD010000114         5808 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWFAD010000114 DAWFAD010000000
VERSION     DAWFAD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.114"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(238..345)
                     /locus_tag="WGO49_001594"
                     /pseudo
     CDS             complement(238..345)
                     /locus_tag="WGO49_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(391..1119)
                     /gene="pdxJ"
                     /locus_tag="WGO49_001595"
     CDS             complement(391..1119)
                     /gene="pdxJ"
                     /locus_tag="WGO49_001595"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ0306687.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQEKIAGFTKILTDAGIRVSLFIDADDRQIQAAYDVGAPVVELHTGAYADAR
                     SHAEQLKQFERLQNGAHFAGDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(1143..1934)
                     /gene="recO"
                     /locus_tag="WGO49_001596"
     CDS             complement(1143..1934)
                     /gene="recO"
                     /locus_tag="WGO49_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ0306688.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLHADGTGGDI
                     LADKTYRLMPEEAVMPVCEDADALSHEAGAIVEGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(1968..3095)
                     /gene="pheA"
                     /locus_tag="WGO49_001597"
     CDS             complement(1968..3095)
                     /gene="pheA"
                     /locus_tag="WGO49_001597"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ0306689.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(3134..4366)
                     /locus_tag="WGO49_001598"
     CDS             complement(3134..4366)
                     /locus_tag="WGO49_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ0306690.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLAVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYHVTPHQYAWAFALNIITMMFFNRITAWRL
                     KTGAHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            complement(4886..5740)
                     /locus_tag="WGO49_001599"
     CDS             complement(4886..5740)
                     /locus_tag="WGO49_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0306691.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGVGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT         1355 a         1687 c         1575 g         1191 t
ORIGIN      
        1 acataatcat cgcctcaata tttaatatcc caaaatatag atttaaaacc atcgcctttg
       61 tggtttgtca gcattcctaa ttgagataga aagtcaaata atttcttgtt tctccggctg
      121 ccatcaagat ggtttagatt aagacctcga acaatacaat acccaatact ctgtactgta
      181 ttgtgaattt cttctatcaa actattattg tttgcagata aaatatactt ttgattcata
      241 tagtgaatta aatttaaacc agtacggcgt tggcttgcct tgccgtacta tttgtactgt
      301 ctgcggcttc gccgccttgt cctgatttaa atttaatcca ctataacata tccctgtcgt
      361 gaaatgctgt ctgaaagggt ttgcctgccc ttacggcagc agccttgccc tgaacatcgc
      421 ctccttcatt tggcgcacgg cttcgggcag tccgaggaag agggcttggg caatcagcga
      481 atgcccgatg ttcagttcgc ggatggcgag gatttgggcg atgggggtaa cgttgtgtat
      541 ggtcagtccg tgtccggcgt tgacgaccaa gcccaaatcg ccggcaaaat gcgcgccgtt
      601 ttgcagacgc tcgaattgct tgagttgttc ggcgtggctg cgcgcgtcgg catacgcgcc
      661 tgtgtgcagc tcgacaacgg gcgcgccgac atcataggcg gcttggattt gcctgtcgtc
      721 ggcatcgata aagagagaga ctcggatgcc tgcgtcggtc aggattttgg tgaacccggc
      781 gattttttcc tgttgcgcca atacgtccaa gccgccttcg gtcgtgattt cctgacgttt
      841 ttcaggcacg atgcacacgt cttccggcat cactttaagc gcgttttcga gcatttcttc
      901 cgtcaacgcc atttcaaggt tcaggcgcgt gcggatggcg tttttgacgg caaacacatc
      961 cgcgtctttg atgtggcggc ggtcttcgcg caggtgcatg gtaatcaaat ccgcaccgtg
     1021 cgtttcggca accagtgccg cctccacggg gctgggataa gtcgtgccgc gcgcattgcg
     1081 gacggtagcg atgtggtcga tgttgacacc taaaagcata atctttcctt ttatttctgc
     1141 cttcagacgg catttgaagc cgtgccgtcc gaagtcggga cggtttcccg ggcggtttct
     1201 ttgcggtcaa actgccgtat ctgttccaac acctgccgcg atttcagccc ctcgggcaac
     1261 aggtggcgga taaaaagccg tgtgattttc aatgcctgtt gcaggctttc ggcggtgcgg
     1321 aaactgcctt cgcgcaaatc gatcaggctt tgcccttcga caattgcgcc ggcttcgtgc
     1381 gaaagcgcgt ctgcgtcctc gcatacgggc atgaccgcct cttcgggcat cagccggtag
     1441 gttttgtccg ccaaaatatc gccgcccgtg ccgtcggcgt gcaaatcggg ggcaacgccc
     1501 aacaggttca gcagccgcca ctcgaaacgg cgcaagtcgt cgatataagc ggctttgcag
     1561 cacaccgcct ccatcacttc cgccaacgcg tcgtataact cgggcaacgg atcttcgcgg
     1621 acggtcaact tcagcatcaa ctcgttcaca tacaatccgc tgaacaaagc cctaccctgc
     1681 ggctgcggcc acccgcctat ccattcggcg cgatgtaggg tcttcaactc ttgcgaacca
     1741 taccaagacg cactcaccgg cacaaacggc accaacacgc cgcgcaattc gctctgccgt
     1801 ttgcgcgcgc ttcttgccaa aagtgccaca cgcccgtaac ggcggctgaa cgcttcgacc
     1861 cgcaggctgc tttcgcgcca gggcgaggaa gccaacagga agacgggttc gtggttgact
     1921 cggtgttcgg acatcatgcc tgaaatgccg tctgaacgct gccgccgcta caaaacggcg
     1981 gtcgggtacg aaccgatgac tttgacgaac gaagcgcgtt cgcccaagcg ttccaatgcc
     2041 gtctgaatct gcgcgtcccg gcggtgtcct tcgatgtcga tgaagaacag gtattcccac
     2101 aaaacggatt tactcggacg gctctcaaac ttggtcatgg aaatacccga ctcagtcagc
     2161 ggttgcagca gcgaggcaac cgcgcctgcc cggttgggcg cggaaacggc cagcgaagtc
     2221 ttgtcgctgc cgcttgcacc ggtttcgtga tgtcccatca ccaagaagcg cgtggtgttg
     2281 ttcggttcgt cttcgatgca ctcggcaacc atatcgagtc cgtagatttc cgccgccgtg
     2341 cgtccggcga tggcggcaac cgtaccgtcg tccgattcgg caaccagcct tgcggcttcg
     2401 gcattgctgg acacggcaat ccgttcggcg ttgggcaggt gtctgcccaa ccagtcgttg
     2461 cactgcgcca acgcctgcgc gtgggaaaag actttggcaa tgccttcggt gctgccgttg
     2521 tttttacgca aaaggttgtg gtggatgcgc aaaacgactt cgccgcacgc ctgcaacgcg
     2581 gtaacggcaa gcaaatccaa cgtgcgcccg accgagcctt cggtcgagtt ttccacgggc
     2641 gcgaccagat aatccgcctg acgggtttca acctgcttga agcagttgtc tatggtcgga
     2701 cacgccatgg tgtgcgcggc gtgtccgaaa tgcttgatcg ccgcctgctg ggtaaacgtg
     2761 ccctgcggcc ccagataggc gatggtcagc gggcgttcga cggcgaggca ctcgctcatc
     2821 acttcccgaa acaggcgtgc taccgattcg tcgggcagcg ggcctttgtt caaatcctga
     2881 atgcggcgca acacggcgac ttcgcgttcg gggcggtaca ctgcgcccgt gcctttcagc
     2941 tcgccgatgg cgtgggcgtg ttgcgcacgt tcgttgagca ggcgcaggat ttcggcatcg
     3001 atggtgtcga tggcgttgcg gtggggaagg aggagttcgt cgatagtttg ggacataatt
     3061 tcgcctgaac agtaatttat cgtgcattct aacatcaaat gccgtctgaa acggtttcag
     3121 acggcatttt ccgttaaagg tattcgctct gttcgttttc cttccacgcc cggtgcgaac
     3181 acagccacag aagcgcaatg ccgcaagagg tggacgcggt catcgttgcc gccatcacgg
     3241 ttgccgaacc gtcgtgcaag aaggtcgccg ccatacccac ccccgcgccg attaaggact
     3301 ggaacacgcc caacacggcg ttcgcgctgc cgccctcttc tttgaaataa gacataaagc
     3361 atgcctgcgt gttcgcgccg accaagccct gcgtaccgac ggaaaacatc acgcacgcaa
     3421 ccagcagcca aaacgggggc aacccgaaaa acagcacggc ggcgagttgg gacaggttgg
     3481 cggcaaattg gacgacaatc ccccacagca ggatgctttg cggatgcgcg ccggttttga
     3541 gccgccacgc ggtaatacgg ttgaaaaaca tcatcgtgat gatgttgagc gcaaacgccc
     3601 aagcgtactg gtgcggcgta acgtggtaga gctgctggta cacgaaggaa gattcggtca
     3661 gaaaggcgaa catcgaaccg aagctgaatg cctgaaaaaa cagataaccc atcgcggcac
     3721 gggttttcaa tacgcgcttg aaccgccccg ccaccagccc gaacacgtcc ctgccgattt
     3781 tgccgccgac ggcgggcttg ggcaggaaat actgtaccaa accgagcagc accagcgaat
     3841 acgccgccag aaaaacaaaa atcgcctgcc agccacccaa gccctgcaac aatgcgccga
     3901 ccatgggtgc gaccagcggc acaaccatca aaatgatgcc gataagggca aacatctggg
     3961 cggctttgcg tccggaataa taatcgcgca ccattgcgcc gacgatgacc acagtcatgc
     4021 ccgcaccgaa tgcctgcacg acgcgcaggt tgaggagctg ttcggcactc gaagcaaata
     4081 cgatggcggc aacggcaagg cagtagacgg ccagtccggt cagcgcgacg ggtttgcgcc
     4141 ctttgatgtc ggacaccgaa ccgccgacca cctgtccgaa cgccgtgccg aacataaaca
     4201 aactcaggct ctgttcgatg cggtggacat ccgcgttcag cgactgcgcc atttcgggaa
     4261 tcgcgggcag gtaggcatcg atggaaaacg gcatcagcgt aaccagcatc gccatcaaaa
     4321 ccgccatcag tttttcgctc atttcaggat aatgggcaga aggcataaat tctcgtccga
     4381 ttcgcaaaca aaccgcccgg cggcggaaaa cacaatacgc ggaaacggaa aattttaaca
     4441 caccggcaca aacaggcggc aacaaaagcc ccggcaaaac cggacgaacc ggaacgatat
     4501 agtggattaa caaaaaccag tacagcgttg ccttgcctta gctcaaagag aacgattctc
     4561 taaggtgctt aagcacccag tgaatcggtt ccgtactatc tgtactgtct gcggcttcgt
     4621 cgtcttgtcc tgatttttgt taatccgcta taaaatcccg tcattcccgc gcaggcggga
     4681 atccggacat ccaacgctgc agcaatttgt cggaaataac cgaaactcaa aaaaccggat
     4741 gcccgcctgc gcgggaatga cgatgcaaag atggtcgtta tttcggataa atatccgtct
     4801 tttcgttccg gatacccgct ttcgcaggaa tggagatgga aagatttcag cctattcggc
     4861 aatcatcaaa taccgcttcc caactttatt tttgatgcga cagccggccg gcaaccaagg
     4921 tggcggcgat gacgacaaac acgcccaagg cggacacggg cgacaggtgt tcgcccaaaa
     4981 tcaaaaccgc cagcagcacg ccgacgacgg gttcgagcga aatcaacagt cccgacacgt
     5041 tggcaggaac acggctcatc cccttgttcc acagccaata ggcgtaccag ccgcacccca
     5101 cgcccaaata cagcagcgac aataccattc cgacgctcca gtccacggta taactttgcg
     5161 ccaaagcaag cgaaaacggc aggcacatca acgatgcggc ggcaatggaa acagatgtga
     5221 atgccggtgc gccgatgcgt gcaatcagcc tttgcgtcgg acgcatagcg gcacaaaagc
     5281 ccgcgcccgc caacaacacc agcaggcagc cgaaccagcc gacttcgccg ccctcttccg
     5341 caccgcccgc catcagcagc gcgacaccgg caaatgccgc cgcgccgcat atccagtggt
     5401 aggcacgcgc tttgtcgttg aagaaaaagt gtccgacaaa caccatcagc agcggctcga
     5461 gtccgacaat gaccgatgcg ctggcggcgg aagtgtattt caacccgaca aactgaagca
     5521 gcagggtcag cacatagttg acgaacgaca caatcagcaa cggcttccat tcctcacgcg
     5581 gaatcttgcc gacatgacgg cggcaggcgg gcagtgcagg cagcgcagca atcagcaggc
     5641 gcacgccgac catcaatgcg ggatcgatgc cgccatagac atatttggcg gcaataaacg
     5701 agctgctcca gataatcagg gcaaggattt ggtaaaacat agtgagtgat aaagtcagat
     5761 ggtcatacaa aagtatagcg gattaacaaa aatcaggaca aggcgacg
//
LOCUS       DAWFAD010000115         5804 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWFAD010000115 DAWFAD010000000
VERSION     DAWFAD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.115"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(103..690)
                     /locus_tag="WGO49_001600"
     CDS             complement(103..690)
                     /locus_tag="WGO49_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ0306692.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTSKGQGKPAGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            853..2259
                     /gene="dnaB"
                     /locus_tag="WGO49_001601"
     CDS             853..2259
                     /gene="dnaB"
                     /locus_tag="WGO49_001601"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ0306693.1"
                     /translation="MNDYAAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMSGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            2414..3091
                     /locus_tag="WGO49_001602"
     CDS             2414..3091
                     /locus_tag="WGO49_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ0306694.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGTVNNKCDSGKKGQGMLAFGDKNGNK
                     GYDNDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTTDRVVWTFNQNGTFGYTK
                     DQHLTKQSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRGDRRTR
                     RTMCQYK"
     gene            3115..3726
                     /gene="pilV"
                     /locus_tag="WGO49_001603"
     CDS             3115..3726
                     /gene="pilV"
                     /locus_tag="WGO49_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ0306695.1"
                     /translation="MKNNDCFRLKDSQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMRNHTLSAVDGDFAIDAI
                     KTKAQLAEEQLKRFSYELKNALPDAAAIRYAVCKDSLGAAPTLSAGSTFSPNCDNKAN
                     GDTLIKVLWVNDSAGDSDIARTNLEVSGDNIVYTYQARVGGRE"
     gene            3723..4661
                     /locus_tag="WGO49_001604"
     CDS             3723..4661
                     /locus_tag="WGO49_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ0306696.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHTEKDVVSDVAQKNRL
                     FSLKRNSTNKLIPITESLNIGYPGFTQRLNALIFQYGIDDVNASADTTVVSSCAKIAK
                     PGKKISTLQEAKSALQITNDDKQNGNITRQRHVVNAYAVGRIAGEEGLFRFQLNDDGQ
                     WGNPQLLVKKINKMDIRYIYVSGCPEDDDAGKEETFRYTDKFNSAQDAVTPAGVEVLL
                     SSGTDTKIAASSDNHIYAYRIDATIRGGNICANRTL"
     gene            4640..5233
                     /locus_tag="WGO49_001605"
     CDS             4640..5233
                     /locus_tag="WGO49_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ0306697.1"
                     /translation="MRKQNALTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRFCPANSTDLCIDKKGMEYKKGTRSVSKPPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            5238..5711
                     /locus_tag="WGO49_001606"
     CDS             5238..5711
                     /locus_tag="WGO49_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ0306698.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSSDVGCEAFSNRKK"
BASE COUNT         1582 a         1282 c         1501 g         1439 t
ORIGIN      
        1 aagtgggaat ctagaaataa aaagcagcag gaatttatcg gaaataactg aaattcaata
       61 ccgcaaaaat ctacccgaaa tgatatagcg gattaacaaa aatcaggaca aggcggcgaa
      121 gcgtttggcg acttcgtccc agttgacgat ttcccagaaa cctttcaggt agttgggacg
      181 gctgttgcgg tagtcgatgt agtaggcgtg ttcccacacg tcgcaggtca gcagcggcgt
      241 gttttcagtg gtcagcggcg tagcggcgtt ggaagtagaa accaaatcca atccgccggc
      301 aggggttttt accagccacg cccaaccgga accaaaagta ccggccgcgc aggcattgaa
      361 cgcttcttgg aatttctcga agctgcccca tttcgcgtcg atggcggcgg ccagttcgcc
      421 ggcaggtttg ccttgacctt tggacgtgaa acccagccag tagaaggtgt ggttccaagt
      481 ttgtgccgcg ttgttgaaca cgccgcccga agattttttc acaatctctt ccaaaggcag
      541 gttttcaaat tcggtgcctt tgatttgatt gttcaggttg gtgatgtagg tttgatggtg
      601 tttgccgtag tggaattcca aagtctcttt gctcagatgc ggggacaatg cgtccagttc
      661 ataaggcagt tgcggcagct tatgttccat tttgtgctcc taatattgtt ttaaatgttg
      721 tattttggca gtgttgctgc aaatagcttg gcagcgcaca tattttacct gttttgaggg
      781 atggaaacca attaaacctg ctttacgcta taatagaaga ttgcaatttc ggcacgacag
      841 ataggatgta ccatgaacga ttacgcagcc atgccgtctg aagaccgtga ggtcggcgca
      901 ttgtcgctgc ctccacattc gatggaggcg gaacaatccg ttttgggtgg gttgatgctg
      961 gaaaatccgg cttgggacag gattgccgat gtggtttccg gtgaagactt ctaccgccac
     1021 gaacaccgcc tgattttccg atccattgcc aaattaatca atgagagccg tcccgcagat
     1081 gtgattacgg ttcaggaaga tttgcagcgg aacgaagaat tggaagcggc aggcggattt
     1141 gaatatctga ttacgctggc gcaaaacacc ccgtctgccg ccaacatccg ccgctatgcc
     1201 gaaatcgtgc gcgagcgttc catcatgcgc cagctcgccg aagtggggac ggaaatcgca
     1261 cgcagcgcat acaatccgca aggcagggac gcggggcagc ttttggacga ggcggaaaac
     1321 aaagtattcc aaatcgccga aagcacggcc aaatccaagc agggcttttt ggagatgccc
     1381 gatttgctga aagaagtcgt acagcgcatc gatatgctct actcgcgcga caatcccgat
     1441 gaagttaccg gcgtgccgac ggggttcatc gacctcgaca aaaaaacctc gggcctgcaa
     1501 cccggcgacc tgattatcgt cgccggtcgt ccttctatgg gtaagaccgc cttttctatc
     1561 aatatcgccg aacacgttgc cgtagaaggc aagctgccgg ttgccgtctt ctcaatggag
     1621 atgggcgggg cgcaactggt catgcgtatg ctcggctcgg tcggacgctt ggatcaaagc
     1681 gtcctgaaaa ccggcaggct cgaagacgaa cactggggtc gtctgaacga agcagtcgtc
     1741 aaactctccg acgcgcccgt gtacatcgac gagaccccgg gtttgactgc gctcgaactg
     1801 cgcgcccgcg cccgtcgtct cgcccgtcaa tttaacaata agcttggatt aatcgtcatc
     1861 gactacctgc aactgatgtc gggttcgggg cgttcggaca accgagcttc ggagctggga
     1921 gagatttcac gttcgctcaa agcgttggcg aaagaattgc aagtccccat catcgccctg
     1981 tcgcaattga gccgtaccgt cgaatcgcgt accgacaaac gcccgatgat gtccgacctt
     2041 cgcgagtccg gcgcaatcga gcaggatgcc gacctgatta tgtttatgta ccgcgacgaa
     2101 tactacaacc aagactcgcc catgaaaggc cttgccgaat gtatcatcgg caaacaccgc
     2161 aacggtcccg tcggtaaaat cttcctcaca tggacgggac aattcaccaa attcgacaat
     2221 gctgcctata ttcccgagga ggcaaagata gaagattaaa tggctatata aaaatttatt
     2281 aggcgaaatc aggcaaaatc gtttaaaatc atgctgagag attgccctaa aaaataaaac
     2341 gcggtcttga ggcatttttg cattcagccc gcatataatt gaaaatacat taccagttaa
     2401 cgtcctatcg cttatgtgta cacgaaaaca acaaggtttc acgctaacag agctgctcat
     2461 cgtgatggtc attgcagcca ttatggcgat gatagccctc cccaatatga gccaatggat
     2521 tgcatcccgc cgcattgcca gtcacgcgga gcggattgcc aaccttttgc gtttctccag
     2581 gggcgaagcc gtccggctca atctccctgt ctatatctgt cctgttcaag ttaaaaaaga
     2641 cggtacggtc aacaataaat gtgactccgg caagaagggg cagggaatgt tggctttcgg
     2701 cgacaaaaac ggcaataagg gatatgacaa tgatacggcg gatgttcttc tccgcagtgt
     2761 ggtattgaat gatgatatca atgataagcg gattaattat gccttcaacc atatcgcttt
     2821 tggtcagact cagccgacca ccgaccgtgt agtttggaca ttcaatcaaa acgggacgtt
     2881 cggttatacg aaagaccagc atcttacaaa acaatccagc tttttttatt ccgacggtta
     2941 tatccaaatc gtgctgacag atgcgagagc agtttcagat gccgataaga aattccgttc
     3001 ggcggtggtt ttgattgaca gcagcggcag ggtcgaagtt tgccctaggg gtgatcggcg
     3061 tactcggcgt actatgtgcc agtataaata acagtttcag ttttaaaaat gaatatgaag
     3121 aataatgatt gcttccgcct gaaagattcc cagtccggta tggcgctgat agaagtcttg
     3181 gttgctatgc tcgttctgac catcggtatt ttggcgttac tgtctgtgca gttgcggaca
     3241 gtcgcttccg tcagggaggc ggagacacaa accatcgtca gccaaatcac gcaaaacctg
     3301 atggaaggaa tgttgatgaa tccgaccatt gattcggaca gcaacaagaa aaactataat
     3361 ctttacatga gaaaccatac actatcagct gtggatggcg attttgcgat tgatgccata
     3421 aaaactaagg cgcagttggc agaggaacaa ttgaagagat ttagttatga gctgaaaaat
     3481 gccttgccgg atgcggcagc catccgttac gccgtctgca aggattcgtt gggtgctgcg
     3541 ccgacattgt ccgccggcag tactttttct ccaaattgcg ataataaggc aaacggagat
     3601 actttaatta aagtattgtg ggtaaatgat tcggcagggg attcggatat cgcccgtacg
     3661 aatcttgagg tgagcggcga caatatcgta tatacctatc aggcaagggt cggaggtcgg
     3721 gaatgagacg taaaatgcta aacgtaccaa aaggcagtta tgatggtatg aagggtttta
     3781 ccattattga atttttggtt gcgggcatgc tcagtatgat tgtcctgatg gcggtcggat
     3841 cgagttactt cacatcccgg aaattaaatg atgcggcaaa cgagcgtctt gctattcaac
     3901 aggatttgcg gaatgcggca acattaattg tccgcgatgc aagaatggcg gggagcttcg
     3961 gttgtttcaa tatgtctgag cataccgaaa aggatgttgt ttccgatgtg gcgcaaaaaa
     4021 accgtctttt ttccttaaaa aggaacagta caaataaact tatccccata acggaatctt
     4081 taaatatcgg atatccgggt tttacccagc gccttaacgc attgattttc caatacggaa
     4141 tcgatgatgt taatgcaagc gccgatacta ccgtcgtcag cagctgtgcc aaaatagcaa
     4201 aaccgggtaa gaaaatatct accttgcaag aagcaaagag tgcattacag attactaatg
     4261 atgataaaca aaatggaaat atcacccgtc aaaggcatgt ggtcaatgcc tatgcggtcg
     4321 gcaggattgc cggtgaggaa ggtttgttcc gcttccaatt gaatgatgat gggcagtggg
     4381 gtaatcctca gttgttggtt aaaaagatta ataaaatgga tatacggtat atttatgttt
     4441 ccggctgtcc tgaagatgac gatgccggca aagaggaaac attcagatat acggataaat
     4501 tcaacagcgc ccaagatgct gttacgcccg ccggggtgga ggttttattg agtagcggta
     4561 ctgataccaa gattgccgct tcttcagaca atcatattta tgcttaccgt atcgatgcga
     4621 caatacgcgg gggaaatata tgcgcaaaca gaacgctttg acgggaatcc cgacttctga
     4681 cggacagagg gggttcgcgc tgtttatcgt gctgatggta atgatcgtcg tggctttttt
     4741 ggttgtaact gccgcgcagt cttacaatac cgagcagcgg atcagtgcca acgaatcaga
     4801 caggaaattg gctttgtctt tagccgaggc ggctttgcgg gaaggcgaac ttcaggtttt
     4861 ggatttggaa tatgatacgg acagtaaggt tacatttagc gaaaactgtg gaaaaggtct
     4921 gtgtaccgca gtgaatgtgc ggacaaataa tgataatgaa gaggcttttg acaatatcgt
     4981 ggtgcaaggc aagcccaccg ttgaggcggt gaagcgtttt tgccctgcaa attctaccga
     5041 cctgtgcatt gacaagaaag ggatggaata taagaaaggc acgagaagcg tcagcaaacc
     5101 accgcgctat attatcgaat atttgggcgt gaagaacgga gaaaatgttt atcgggttac
     5161 tgccaaggct tggggtaaga atgccaatac cgtggtcgtc cttcaatctt atgtaagcaa
     5221 taatgatgag taataaaatg gaacaaaaag ggtttacatt gattgagatg atgatagtcg
     5281 tcgcgatact cggcatcatc agcgtcattg ccataccttc ttatcaaagt tatattgaaa
     5341 aaggctatca gtcccagctt tatacggaga tggtcggtat caacaatatt tccaaacagt
     5401 ttattttgaa aaatcccctg gacgataatc agaccatcaa gagcaaactg gaaatatttg
     5461 tctcaggcta taagatgaat ccgaaaattg ccgaaaaata taatgtttcg gtgcattttg
     5521 tcaataagga aaaaccaagg gcatacagct tggtcggcgt tccaaagacg gggacgggtt
     5581 atactttgtc ggtatggatg aacagcgtgg gcgacggata caaatgccgt gatgccgctt
     5641 ctgcccgagc ccatttggag accttgtcct cagatgtcgg ctgtgaagcc ttctctaatc
     5701 gtaaaaaata gggctgtttt gccgatgccg tctgaagatt aattttcaga cggcattttt
     5761 atgggtatag tggattaaca aaaatcagga caaggcgacg aagc
//
LOCUS       DAWFAD010000116         5700 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWFAD010000116 DAWFAD010000000
VERSION     DAWFAD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5700
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.116"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            309..1256
                     /locus_tag="WGO49_001607"
     CDS             309..1256
                     /locus_tag="WGO49_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0306699.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMTYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVALHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1408..2532)
                     /gene="rluD"
                     /locus_tag="WGO49_001608"
     CDS             complement(1408..2532)
                     /gene="rluD"
                     /locus_tag="WGO49_001608"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ0306700.1"
                     /translation="MQNTSFDNESDYSDDSDFASASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTLWIKEGAVIVNDKPSQPKDKMIGGEQIRVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWRGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLTHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NPRHPCSDPVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2531..3334
                     /locus_tag="WGO49_001609"
     CDS             2531..3334
                     /locus_tag="WGO49_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ0306701.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3392..5611)
                     /locus_tag="WGO49_001610"
     CDS             complement(3392..5611)
                     /locus_tag="WGO49_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ0306702.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTAVRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVAAAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRIRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1358 a         1814 c         1380 g         1148 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat atgtttcaac aggggatggc
       61 acatcaagca ccgcactgcg tgttgcccga acgagactac cctccgatta gattctatcg
      121 ctataaacag acgggtttca accgaaaagg aatggggata aagtccattt ctgacacctc
      181 tcgggcgatg ccgtctgaaa accaatctcc actttcagac ggcattgttt aggcacggtc
      241 attttgcaaa ttaaggttaa ggcgtgtatg cttgccttcc cttaaaaaca aacacgcaac
      301 gcattattat gaatatcctc agtaaaatca gcagctttat cggaaaaaca ttttccctct
      361 gggccgcgct ctttgccgcc gccgcttttt tcgcgcccga caccttcaaa tgggcggggc
      421 cttatattcc ttggctgttg ggcattatta tgttcggtat gggtttgacg ctcaaacctt
      481 ccgacttcga tattttgttc aaacatccca aagccgtcat catcggcgta atcgcacaat
      541 tcgccattat gccggcaacc gcctggctgc tgtccaaact gttgaacctg cctgccgaaa
      601 tcgcggtcgg cgtgattttg gtcggctgct gcccgggcgg tacggcttcc aatgtgatga
      661 cctatctggc gcgtggcaat gtggctttgt cggttgccgt tacgtctgtt tccaccctga
      721 tttccccatt gctgactccc gccatcttcc tgatgcttgc cggtgaaatg ctggaaatcc
      781 aagcggccgg tatgttgatg tccatcgtca aaatggtttt gctccccatt gttttgggtt
      841 tgattgtcca taaggttttg ggcagtaaaa ccgaaaagct gaccgatgcg ctaccgctgg
      901 tttccgttgc cgccatcgtg ctgattatcg gcgcggttgt cggggcaagc aaaggcaaga
      961 ttatggaaag cggcctgctg atttttgccg ttgtcgcact ccacaacggc atcggctacc
     1021 tgctcggctt ctttgccgcc aaatggaccg gcctgcctta tgatgcacaa aaaacgctgg
     1081 ccatcgaagt cggtatgcaa aactcggggc tggccgccgc gcttgccgcc gcacactttg
     1141 ccgctgcgcc ggtcgttgcc gttccgggcg cattgttcag cgtgtggcac aatatctccg
     1201 gctcgctgct ggcaacttat tgggcggcca aagccggtaa acataaaaaa ccctaaacgc
     1261 ctttcatcat aatgccgtct gaacggggca aacgaaaccg gcggtttctg cccgacccct
     1321 tcagacgcct ttgtttgaaa tcggcgggga cgattatccg gctgctccga ttcggctacc
     1381 tgcgttcgcc ccgcctttca agccacctca ctccctcaca taaaccactt cgacatcgta
     1441 gtcgtcttcg ttccaatcgt catcgtcgtc cgcgccgaat ttgtcctgcc attcctcttc
     1501 attgctcaaa gacgaatcca aaccggcttc aagacggagg acggacaaca aatgatatat
     1561 gtcgtccgga atcggtgcct caaacgaaac ggtttcgccg ctttccggat gggtgaaact
     1621 caagcggtag gcgtgcaatg cctgacgcgc gcccaaactt ttaacggctt ctttcaccgg
     1681 gtcgctgcac ggatggcgcg ggttgccgta cacgggatcg ccggcaagcg gatggttggc
     1741 ctcgcgcata tggacgcgga tttggtgcgt cctgcccgtt tcgagcgagc attcgatata
     1801 gctgtgggta agatagcgtt ccaacacttt gacgtgggta acggctggtt tgccgccgaa
     1861 tttgacgact gccattttca ggcggttgtg cggatcgcgt ccgatttggg tttcgatttt
     1921 accgtcgaag gggacgatgc cgttggcgac ggcgcggtag atgcgtttga ccgtgcgctc
     1981 ttgaagctgc ctcacgaggg aattttgcgc cggcagggtt ttggcaacca ccatcagccc
     2041 gctggtttct ttgtccaaac ggtgtacgat gcctgcacgc ggtacttggc tcaattcggg
     2101 acagtgcgcc aacaggccgt tgagcagcgt ccccctccag ttgcctgccg ccggatgcac
     2161 caccagtccg gccggtttgt tgacgacaat gacggtatcg tcttcgtaaa caatatccaa
     2221 agccataggc tctggaacaa acgccagatt ttcctcactc ggacggacgg ttacacggat
     2281 ttgctcaccg cctatcattt tgtctttggg ttgcgaaggt ttatcgttta caataaccgc
     2341 gccctcttta atccataatg tcaggcggct gcgcgaatag tcgggcagaa gtttcgccaa
     2401 caccgcatcc aaccgcccgc cggcaagctc gagcggaacg gtcagaccga tacggttttc
     2461 agtttcggaa gctgacgcaa agtctgaatc gtcgctataa tcggattcat tatcaaagga
     2521 agtattctgc atgaaaaaaa ttcttttaac ggtttcatta ggtttggcac tgagtgcctg
     2581 tgccactcaa ggtacggtcg ataaagatgc ccagattacc caagattgga gtgtggagaa
     2641 gctctatgcc gaagcccagg acgaattgaa cagcagcaat tatacgcggg ctgtcaagtt
     2701 atacgaaatc ttggaatcgc gcttccccac cagccgccat gcccggcagt cgcaactgga
     2761 taccgcatac gcctattata aagacgatga aaaagacaaa gctctggcgg caatcgaacg
     2821 cttccgccgc cttcacccgc aacacccgaa tatggattac gcgctatacc tgcgcggttt
     2881 ggtactgttc aacgaagatc agtctttctt gaacaagctg gcctcgcaag actggtccga
     2941 ccgcgacccg aaagccaacc gcgaagcgta ccaggcgttt gcggaactcg tccaacgctt
     3001 ccccaacagc aaatacgccg ccgatgcgac cgcacgtatg gtcaaactgg tcgatgcact
     3061 gggcggcaat gaaatgtcgg tggcgcgcta ctacatgaaa cgcggtgcat atatcgccgc
     3121 cgccaaccgc gcccaaaaaa tcatcggcag ctaccagaat acgcgttatg tcgaagaatc
     3181 gctcgccata ttggaacttg cctaccaaaa actcggcaaa ccacagcttg ccgccgatac
     3241 gcgccgcgtg ttggaaacca acttcccgaa aagcccgttt ttgacgcacg cttggcagcc
     3301 cgacgatatg ccctggtggc gttactggca ttaagcccat cttcgtaatg ccgtctgaag
     3361 ccgtttcaga cggcatttta tgtttcaaac ctcattcaaa cggttttttc tgccaataga
     3421 atttataccc tctcaaacgt tgagccttca cgccgccgcg ccccaagccg aattgcagcg
     3481 cgcccgacaa atcggtacgc aacaatttga tgccgtgtgc acggatacgg ttttgcaccg
     3541 cttcggtcgg atgtttgtag gcgttggcat aaccgcttga agcaacggca tattcgggcg
     3601 agacggcatt gaggaacacg cccgacgagg acgtattgct gccgtgatgt cccaaaacca
     3661 aaacctggct gtacaggttg cctccatact tgccgaccag gctttcctcg cccttcgtat
     3721 ccaaatcgcc cgttaccagc agtgccgcac cgcccgccac aacacgcaaa acacaacttt
     3781 tcccattatc atcgatgttt ttgcgttcag acggcatcaa aaactcaaaa tccacgccgt
     3841 cccattgcca acgctgttcc gcacaatgcc gcgccccctc ataaaattcc ggctgcccgg
     3901 cataaatccc gccgttcggt atcttgccga ctgcctgaaa accgccgtcg tggtcgctgt
     3961 cgtgatgcga cagaaccagc ttgtccaaac ggcggacacc cgccgcattc aaactcggca
     4021 caatccccat ctgcgccgcc gctgccgttc cggtgtcaaa caaaagatga tgatttgccg
     4081 tctgaaccga caccgacaaa ccctgccccg catcccaaac cgtaaccgca acctcatttt
     4141 ccggcacgcc gggtgaacgg taaaacacaa accctgccaa cagcaacacc gcccacggac
     4201 gcaaacccaa gccgcgcggc agcagcaaca gcaaagcggc acacaccgcc aacaccaaca
     4261 gcggcaaagg tgcggcggca acggcaaact cgggcgacac atcggcaagc cacaccaaaa
     4321 accgcaaagt atattccgcc aaaaatgccc ccaactgttg caaaggcgca aacggcacga
     4381 ccgaacccag caacgccagc ggcgtcaata cccaagaaaa ccacggaatc gccaccgcat
     4441 tgaccaaagg gctgattaaa ggcagcgaag caaacagata accgagcaaa accagcgaca
     4501 acaccgaagc cgcccactgc ccgcgcacgg cggtttgccg tttcccttcg tgcaaacgcc
     4561 ccgaacacgc ccatatcagg gccgccacca aaccgaaaga cagccaagtc cccacaccca
     4621 agaccgccga cgggtcgaac agcagcaccg ccgccaacgc ctgccaccac gtcgcccacg
     4681 ccgacaatct tcccctgcgc caagcccacg caaacgccgc caacatcaaa acgctgcgct
     4741 gcgtcggcac ggaaaaaccg gcaagcagcg cgtaaaacag cgcgcctgca caccccgccg
     4801 ccaaaaccca cgcacgcggc cgggcaggaa ggcgcgggga acaggcaagc agccgcttcg
     4861 ccagccacgc aaacattacc gccaccatcg taacgtgcaa acccgaaata ctgaccaaat
     4921 gcgtcagccc caacggccgg aacgcctgcc acaattcggg gcgcaatgcc gactgttcgc
     4981 ccacgctcaa cgcgcgcatc agcccgatgc cgtctgaaag cccgccgtcc gcatccgcct
     5041 gctgccaatt acggctgatg cggctgcgcc aaaccgcaat cccccaaccg cttccgccat
     5101 gcagcaaaac cctgtccgca ccgaccgtcc ccgcgccgcc tatcccgttg gataatgccc
     5161 acgcctcacg gttcaaaccg cgcaggttca attcgccgac gacggggtgc acacgtgccg
     5221 ttatccgcca tctgctcccg accgcccatt cgcgccgttt gtagtccgac agcagcaaat
     5281 caaacgtccg accaccgctg tccacagcct ttgccgcaaa ctgcacgcgc cgcccgtccg
     5341 acctcggcat atccgccact tccaccgtca acggcacacc tgaaaccgcc tccgcccgcc
     5401 attgcgaaga cagtgccgct tccgttctga atacgccgta agccgcgccc gccaacacgc
     5461 acagcatcag accggcaaac gcaaaacgcc ttgcaaacac agcaaaaacc gcaaaagccg
     5521 ccagccaaaa cagccaatgc ggcacgaccg gcagcgcaaa cgaagccgcc gcgccgacaa
     5581 cccaacacgg caaaaaccaa cgacggaaca cgtctcccca ccccagatat ttcaaaaata
     5641 aacggataat aaagcaaatc cgaaaaaccg tcataacaag atttgaaaat gatatagtgg
//
LOCUS       DAWFAD010000117         5675 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWFAD010000117 DAWFAD010000000
VERSION     DAWFAD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.117"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(81..842)
                     /locus_tag="WGO49_001611"
     CDS             complement(81..842)
                     /locus_tag="WGO49_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306703.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKVVPTYAPLSRPSETGTDASVP
                     KLDDASRNRVLKAYLKKWQEEHNAAVADCGEEIETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRIRFDTKKDSVEFETGQIEGGENADEAESGTNEPAVSENGENDAEETLMPV
                     QLNILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            942..1193
                     /locus_tag="WGO49_001612"
     CDS             942..1193
                     /locus_tag="WGO49_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306704.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEVA
                     LESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1245..2051
                     /locus_tag="WGO49_001613"
     CDS             1245..2051
                     /locus_tag="WGO49_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ0306705.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALEKVRESGEYDKIYAKYFAKEDEQAAK"
     gene            2358..3881
                     /locus_tag="WGO49_001614"
     CDS             2358..3881
                     /locus_tag="WGO49_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ0306706.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKSIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTGLLGMIGKSERGVATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPRKWQAKIGIIPVES"
     gene            3979..5391
                     /gene="trkA"
                     /locus_tag="WGO49_001615"
     CDS             3979..5391
                     /gene="trkA"
                     /locus_tag="WGO49_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ0306707.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1424 a         1712 c         1383 g         1156 t
ORIGIN      
        1 ttttgcgcct gttccatgag gctttcaagt cggatgagaa tgcaaatgcc gtctgaaacg
       61 gctttcagac ggcatggcaa tcagcgtttg tattttaact cgtacttgat gtcgttgagg
      121 attttgcgga catcgtgttc caacacgtct tcgactaccg cccccgcctg ctcgtgcagc
      181 atctgctgga gctgataggt gaaaaccgcc atctgctttt gcaccgccgt tcggatgatg
      241 ccgttgacgg tatcggtcag atgggggcgc aggcgtttga tcagccgttc ggtcagctcc
      301 tgttcggaca ggcagaacac ttcgcgccgg ttgacggctt tcgggttcag gatattgagt
      361 tggacgggca tcaacgtttc ttccgcatcg ttttccccgt tttccgaaac cgccggctca
      421 ttcgtgccgg attctgcctc gtcggcgttt tccccgcctt caatctgtcc ggtttcaaat
      481 tcgacactgt cttttttggt atcaaaccgg attctccgcc gcgattcgat gtgtttttcc
      541 gaaaccgaca tttgcaggga agcctgcgcg ttgagccagt tttcctgaag gacgatcatc
      601 gggtcggttt cgatttcttc cccgcaatcg gcaacggcgg cattgtgctc ctcctgccat
      661 ttcttcagat acgcctttaa cacacggttt cggcttgcat cgtccagttt cggcacagac
      721 gcgtccgttc cggtttcaga agggcgggac agcggcgcgt aagtcggcac taccttcata
      781 cggcgcgtct gacgcaggtt ttccaaacgt ttttcccaat tcggctcttt attcgcatcc
      841 attttcggct tccggtcctt aatctttgca agcagacaaa cccgcgccca aagcgcggtt
      901 tgatataatg gcgcatttta acagattcgc gaggatacat catgggcagc atcgaacagc
      961 gtttggaata tctggaagag gcgaacgacg tgctgcgtat gcagaaccac gtcctgtcca
     1021 ccgccttcaa agccttaatc cgcgcccttc ccgccgacac cgccgaagtc gcgctcgagt
     1081 ccatccagct tgcttttgag gacgccttgg cagaattgag ctatgaggac agcccgcata
     1141 cggatttgtt ccacgacgtt acttatgcgt ttttccgtga aaaagaacgt taattttatg
     1201 ttaaactgat tttttaggct ttttgattac cgaaaggaat tttgatgaat atgaaaaaat
     1261 ggattgccgc cgcccttgcc tgttccgcac tcgcgctgtc tgcctgcggc ggtcagggca
     1321 aagatgccgc cgcgcccgcc gcaaaccccg acaaagtgta ccgcgtggct tccaacgccg
     1381 agtttgcccc ctttgaatct ttagactcga aaggcaatgt cgaaggtttc gatgtggatt
     1441 tgatgaacgc gatggcgaag gcgggcaatt ttaaaatcga attcaaacac cagccgtggg
     1501 acagcctttt ccccgccttg aacaacggcg atgcggacgt tgtgatgtcg ggcgtaacca
     1561 ttaccgacga ccgcaaacag tctatggact tcagcgaccc gtattttgaa atcacccaag
     1621 tcgtcctcgt tccgaaaggc aaaaaaatat cttcttccga cgatttgaaa aacatgaaca
     1681 aagtcggcgt ggtaaccggc tacacgggcg atttctccgt atccaaactc ttgggcaacg
     1741 acaatccgaa aatcgcgcgc tttgaaaacg ttcccctgat tatcaaagaa ctggaaaacg
     1801 gcggcttgga ttccgtggtc agcgacagcg cggtcatcgc caattatgtg aaaaacaatc
     1861 cgaccaaagg gatggacttc gttaccctgc ccgacttcac caccgaacac tacggcatcg
     1921 cggtacgcaa aggcgacgaa gcaaccgtca aaatgctgaa cgatgcgttg gaaaaagtac
     1981 gcgaaagcgg cgaatacgac aagatttacg ccaaatattt tgcaaaagaa gacgaacagg
     2041 ccgcaaaata agcccgccca tccgaacaca atgccgtctg aagccctttc agacggcatt
     2101 gttcatcaat cggcctacaa tgaactgcct gctgatttct ccctaccgca aagcaacagg
     2161 caaagattac aaatatcaaa atccgagtaa aacagtattt tattaaaaca aattgataat
     2221 caagagatta gaattatgta ttgtctttac cgtacaaacg ctggcactat ttcaacctga
     2281 taaaaaacag ccttcaaaaa ggttgcttaa aacagcagca gacacttacc gccacaacct
     2341 tgaaaaggaa cacaatcatg accgtcatca aacaggaaga ctttatccaa agcatttgcg
     2401 atgccttcca attcatcagc tactaccatc cgaaagacta catcgacgcg ctttataagg
     2461 cgtggcagaa ggaagaaaac cccgccgcca aagacgcgat gacgcagatt ttggtcaaca
     2521 gccgcatgtg tgccgaaaac aaccgcccca tctgccaaga taccggtatc gcgaccgtct
     2581 tcctcaaagt cggtatggat gtgcagtggg atgcggacat gagcgtcgaa gaaatggtta
     2641 acgaaggcgt gcgccgcgcc tacacttggg aaggcaacac cctgcgcgcc tctgtcctcg
     2701 ccgatccggc cggcaaacgt caaaacacca aagacaacac ccccgccgtc atccatatga
     2761 gcatcgtgcc gggcggtaaa gtcgaagtaa cctgcgcggc aaaaggcggc ggctctgaaa
     2821 acaaatccaa actcgccatg ctcaatcctt ccgacaacat cgtcgattgg gtattgaaaa
     2881 gcatcccaac catgggcgca ggctggtgtc ctcccggcat cttgggcatc ggcatcggcg
     2941 gcacgcccga aaaagccgtg ctgatggcaa aagagtccct gatgagccac atcgacattc
     3001 aagagttgca ggaaaaagca gcgtcaggtg cagaattgtc caccaccgaa gccctgcgcc
     3061 tcgaactctt tgaaaaagtc aacgcgctag gcatcggcgc gcaaggcttg ggcggtctga
     3121 ccaccgtgtt ggacgtgaaa atcctcgatt acccgaccca cgccgcctcc aaaccgattg
     3181 ccatgattcc gaactgcgcc gccacccgcc acgtcgaatt tgaattggac ggctcaggcc
     3241 ctgtcgaact cacgccgccg cgcgtcgaag actggcccga tttgacttac agccccgaca
     3301 acggcaaacg cgtcgatgtc gacaagctga ccaaagaaga agtggcaagc tggaaaaccg
     3361 gcgacgtatt gctgttgaac ggcaaaatcc tcaccggccg cgatgccgca cacaaacgcc
     3421 tcgtcgatat gctcaacaaa ggcgaagaat tgcccgtcga tttcaccaac cgcctgattt
     3481 actacgtcgg ccccgtcgat ccggtcggcg atgaagtcgt cggtccggca ggtccgacca
     3541 cagccacccg catggacaaa ttcacccgcc aaatgctcga acaaaccggc ctcttgggca
     3601 tgatcggcaa atccgagcgc ggcgtggcca cctgcgaagc catcgccgac aacaaagccg
     3661 tgtacctcat ggcagtcggc ggcgcggcgt atctcgtggc aaaagccatc aaatcttcca
     3721 aagtcttggc gttccccgaa ttgggcatgg aagccattta cgaatttgaa gtcaaagaca
     3781 tgcccgtaac cgtcgccgta gatagcaaag gcgaatccat ccacgccacc gccccgcgca
     3841 aatggcaggc gaaaatcggc atcatccccg tcgaatcttg aggcgccatg ccgtctgaac
     3901 acaaaatctg ccttcagacg gcatttccgc ccccggttgc ggtacaatcc accatttcat
     3961 cactcggcga cccacaccgt gaaaatcctc attttaggca acggacaggt aggttctacc
     4021 gtcgcacaaa accttgccgc catacccaac aacgacgtaa ccgttatcga catcgacgaa
     4081 aaagcattgc aggaaacagg cagccgcctc gatgtccaaa ccgttttcgg caacggcgca
     4141 tcccccttca cattagaacg cgccggcgcg gaagatgccg acttgctgct cgcgctctcc
     4201 cgcagcgacg aaaccaacat cgtcgcctgc aaagttgccg ccgacctgtt caacatcccc
     4261 ggccgcatcg cgcgcgtccg ttccagcgaa tacctcgaat acctcagccc caagctcgaa
     4321 aacaacgaaa acggcagcct ttccatattc ggcataaccg aaaccatcag ccccgaacag
     4381 ctcgttaccg aacagcttgc cggcctgata gactgcccgg gcgcattgca ggttttacgt
     4441 tttgcagacg accgcgtgcg gatggtcatc atacaggcgc ggcgcggcgg actgcttgtc
     4501 ggacgcagca ttgccgacat cgcccaagat ttgcccgacg gggccgactg ccaaatctgc
     4561 gccgtttacc gcaacaaccg cctcatcgtc cccgcgccgc aaaccgtcat catcgaaggc
     4621 gacgaaatcc tatttgccgc cgccgccgaa aacatcggcg cggtcatacc cgaattgcgc
     4681 cccaaagaaa ccagcacccg ccgcatcatg attgccggcg gcggcaacat cggctaccgt
     4741 ctcgccaagc agctcgaaca cgcatacaac gtcaaaatca tcgaatgccg gccgcgccgt
     4801 gccgaatgga tagccgaaaa cctcgacaac accctcgtcc tgcaaggttc ggcaaccgac
     4861 gaaaccctgc tcgacaacga atacatcgac gaaatcgacg tattctgcgc cctgaccaac
     4921 gacgacgaaa gcaacattat gtccgccctt ttggcgaaaa acctcggcgc gaagcgcgtc
     4981 atcggcatcg tcaaccgctc aagctacgtc gatttgctcg aaggcaacaa aatcgacatc
     5041 gtcgtctccc cccacctcat caccatcggc tcgatactcg cccacatccg gcgcggcgac
     5101 atcgttgccg tccaccccat ccggcgcggc acggcggaag ccatcgaagt cgtcgcacac
     5161 ggcgacaaaa aaacttccgc catcatcggc aggcgcatca gcggcatcaa atggcccgaa
     5221 ggctgccaca ttgccgccgt cgtccgcgcc ggaaccggcg aaaccattat gggacaccat
     5281 accgaaaccg tcatccaaga cggcgaccac atcatctttt tcgtctcgcg ccggcgcatc
     5341 ctgaacgaac tggaaaaact catccaggtc aaaatgggct ttttcggata aaccgcccca
     5401 ttccggacat attgccgcca agcggtatgg aagcggaata atggtaggtg ggcttcagac
     5461 ggcatccgcc ctccccgtca ttcccgcgta agcgggaatc cagaccttgg gatagcggca
     5521 atattcaaag gttataaaag acccgtcatt cccgcgcagg cgggaatcca gaccttggga
     5581 tagcggcaat attcaaaggt tagctgaagc tttagagatt ctagattccc gtttccacgg
     5641 gaatgacgaa aggttgcggg aatccaaacc attgg
//
LOCUS       DAWFAD010000118         5618 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWFAD010000118 DAWFAD010000000
VERSION     DAWFAD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.118"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="WGO49_001616"
     CDS             complement(98..1231)
                     /gene="nrdB"
                     /locus_tag="WGO49_001616"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ0306708.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGMHNVGGKPITVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(1445..2425)
                     /locus_tag="WGO49_001617"
     CDS             complement(1445..2425)
                     /locus_tag="WGO49_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEZ0306709.1"
                     /translation="MTAARAGLVRFNYLKVKQAVQSKQIKGFKLWKSYDEQLEILRSR
                     GLQIDDEKKALGYLRTIGYYRLSGYFYSFRQFAPESSKSRLDEFITDTRFEDVKSLYM
                     FDKKLRQLALDALERIEVALRVNIAHTLGRHDPLAYKDSKYSATGFDHAEWLDRYNRL
                     VEREKKTGFVKHHLEHYADLPVWVACEVWDFGTMSMLYKGMLNKDKDSIAKLYRLKDG
                     KHLQTHLHAFNFIRNVSAHHSRLWNRDITFKASLKGLSDAEWKSLPINKPFVYFCLMK
                     RMLDVICPRTEWGNRLLALLDEFPDVANEAVSLRDMGLENNPRDWRLWQG"
     gene            complement(2391..4670)
                     /gene="nrdA"
                     /locus_tag="WGO49_001618"
     CDS             complement(2391..4670)
                     /gene="nrdA"
                     /locus_tag="WGO49_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ0306710.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKEARLGYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKDAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPNKFEGSKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQADIQDDGCAGGACKI"
     gene            5199..5510
                     /locus_tag="WGO49_001619"
     CDS             5199..5510
                     /locus_tag="WGO49_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HEZ0306711.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFRSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         1406 a         1364 c         1310 g         1538 t
ORIGIN      
        1 gaacaataac cgatcctacc cgctgttttt cccatcatac aacatacaaa tgccgtctga
       61 aacatccggc ttcagacggc attttgtcaa aaaacgttta caactcaaaa tcgcccaaat
      121 catccgtatt cacttcagaa tcgatctgac cgatcaaata agaggatatt tccacttcct
      181 gcggcgcgac ctgtacgttg tcggacgaca gccacgcatt aatccacgga atcgggtttt
      241 gatttgcgcc ttcaaatccg gccggcaacc ccaccgcctg catacgcaga ttggtaatat
      301 attcgacgta ttgggataag atttctttgt tcaaaccgat catcgaaccg tccttaaaca
      361 aatatgccgc ccattctttt tcctgttccg ccgctttttt gaagagttgg aaacattcgt
      421 cctgcaactc ggcggcaatt tctgccattt cagaatcatc aacacccgaa cgcatcagat
      481 taagcatatg ctgcgtgccg gtcaggtgca gggcttcgtc gcgggcaatc agtttgatga
      541 ttttggcgtt gccttccatc aactcgcgct cggcaaaagc aaacgagcag gcgaatgaaa
      601 cgtagaaacg gatggcttcc aacacgttga cgcacatcag gcagagatag agttttttct
      661 tcaacccgcg caaagacaca gtaatgggtt ttccgccgac attatgcatc ccttcgccca
      721 acaggttgta atactgggtg tattcgatta agtcatcgta atagcaggca atgtcttcgg
      781 cgcgggcggt aatgtattcg ttttcgacaa tatcatcaaa cacgaccgac ggatcattca
      841 caatattgcg gatgatgtgg gtatagctgc gcgagtgtat ggtttcgctg aagctccacg
      901 tttcaatcca cgtttccagc tcgggaatcg aaaccaaagg cagcaaggca acattcggac
      961 tgcgcccttg gatggaatcg agcagtgttt ggtatttcag attgctgatg aaaatatgtt
     1021 tttcgtgttc aggcaggttg gcgtagtcga tacggtcgcg cgacacgtcg atttcttccg
     1081 gccgccaaaa aaacgacaat tgtttttcaa tcagtttttc aaatacctcg tatttctgct
     1141 ggtcataacg ggcgacattc acaggctgac cgaaaaacat cggctcattc agcgcgtcgt
     1201 ttttggtttt gggaaaggtg ctgtatgaca tgactaggtg ttcgcaggac atggttttct
     1261 caggtttatt taaatttagg aatttggatt tcagacagcc ttatatgttt taagtaaggt
     1321 catctgaatg tatctgtaca ttgaattctt gccttaaata tcgctgttag ttataatctt
     1381 ccccaacaac tcccccaact ttgccaacct acgcggttgg agcaacaagg ttgggggctt
     1441 tcttttatcc ctgccacaat ctccagtctc gcggattatt ttccaaaccc atatcgcgca
     1501 aacttacagc ttcattcgcg acatcgggaa actcatccag taaagcaaga agacggtttc
     1561 cccattcggt tcgcggacaa ataacatcca acatccgttt catcaggcaa aaatacacaa
     1621 acggtttatt gataggcaat gatttccatt ctgcatcaga caaacctttc aagctggctt
     1681 taaaagtaat atccctattc cataatcgac tgtgatgtgc cgaaacgttg cggataaagt
     1741 taaatgcgtg cagatgtgtt tgcagatgct taccgtcttt caaacgatac aatttggcaa
     1801 tagagtcttt atctttattc aacatccctt tatacagcat agacatcgtg ccgaaatccc
     1861 atacttcgca ggctacccag acaggtaagt cggcataatg ttccaaatga tgtttaacaa
     1921 aaccggtttt cttttcgcgc tcgaccagcc tgttatacct atccagccat tccgcatggt
     1981 cgaaacctgt ggcagaatat ttgctatctt tgtaagccaa tgggtcatgc cgtcctaatg
     2041 tatgggcaat atttacccga agcgctactt caattctttc cagtgcatct aatgccaatt
     2101 gccgcaattt cttatcgaac atataaaggc ttttgacatc ttcaaaacga gtatctgtaa
     2161 taaactcatc caacctgctc ttagaacttt caggagcaaa ttgtcggaaa gagtaaaaat
     2221 aaccgcttaa tcggtaataa ccgatggtgc gtaaatatcc taatgccttt ttctcatcat
     2281 caatctgcaa tcctctactt cttagaatct ccaactgctc atcgtaagat ttccaaagct
     2341 tgaagccttt tatctgcttg ctttgcacag cctgtttcac tttcaaatag ttaaatctta
     2401 caagccccgc ccgcgcagcc gtcatcctga atatcggctt gcgtatcgtc cgcgccgtcg
     2461 cgggtgttgt ggtagtacag ggttttgacg ccgtatttgt aagcggtcaa taagtctttg
     2521 agcatttgtt tcatggaaac tttgctgcct tcgaatttgt tcgggtcgta ggcggtattg
     2581 gcggaaatcg attgatcgac gaatttttgc attacgccga caagtttcaa gtagccttcg
     2641 ttgccgggaa gctgccacag ggtttcgtag gcgtctttca gggtttcaaa ttccggtacg
     2701 acttgtttca aaataccgtc ttttgatgct ttgaccgtta ccaatccgcg cggcggctcg
     2761 ataccgttgg tggcgttggc gatttgcgaa ctggtttcag acggcatgag tgcggttaga
     2821 gtagagttac gcaggccgta tttgacgatt tcggcacgca ggctttccca gtcgtaatgc
     2881 aaaggctcgc cgcagacggc atccaaatct tttttgtagg tgtcgatggg cagtttgcct
     2941 tgcgaataaa cggtttggtt aaagagcgta cacgcaccgt attctttggc aaggtttgcc
     3001 gatgctttga gcaggtaata ctgtatggct tcaaaggtac ggtgggtcag accgagcgcg
     3061 gagccgtcgc tgtagcggac accgtttttc gccagataat aagcatagtt aatcacgccg
     3121 atgccgagcg aacggcggcc catagtagag gtacgcgcgg cttctaccgg atatccctga
     3181 taatctaaaa gtgcatcgag cgcacgaacg gtcaagtcgg caagcccttc caattcgtcc
     3241 aagctgttta atgcgcccaa gttaaaggca gacagtgtac acagggcgat ttcgccgttc
     3301 ggatcgttga tattgtccag cggtttggtc ggcagggcga tttccataca caagttggac
     3361 tgatgaacag gcgcgacgcg cggatcgaac gggctgtgcg tattgcagtg atcgacgttt
     3421 tgaatgtaga tgcgcccggt tccggcacgc tcctgcatca gcgtggaaaa caggtcggca
     3481 gccggaatga tgcgcttgcg gatatcaggg tcttgctcgt atttcgtata gagccgctca
     3541 aattcgtctt ggtcggcaaa aaacgcttcg tacaatcccg gaacctcgtt gggcgaaaac
     3601 agcgtaatgt tgccgccctt aatcaggcgg gtgtacagca ggcggttgat ttgcacgccg
     3661 taatcaagct gacggatacg gttgtcttcc acaccgcggt tgtttttcaa caccagcagg
     3721 ctttcggctt cgatatgcca caaggggtag aacaaggttg ccgcgccgcc gcgcacgccg
     3781 ccttgcgaac aggatttgac cgccgcctga aacattttaa agaagggaat gcagccggta
     3841 tgccgcgctt cgccgccccg gatttcgctg tccaaaccgc ggatacgtcc ggcattgatg
     3901 ccgatgcccg cacgctggga aacgtatttc acaatcgcgc tggtagtggc attgatggaa
     3961 tccaaactat cgtcgcattc aatcagcaca cagcttgaga actggcgcgt aggcgtacgc
     4021 acgccgctca taatcggagt cggcagcgat actttaaatg tagaaacggc atcgtaaaac
     4081 cgtttgacgt aacccaagcg cgcctctttc gggtatttgc tgaaaaggca catcgccacc
     4141 aaaacatata aaaactgcgg cgtttcgtaa atttggcggg taacgcggtt ctgtaccaga
     4201 tatttgcctt cgagctgttt gacagcggca taggaaaagg acatatcgcg ttcgtggtcg
     4261 atataggcgt tcagttcgtc aaattcttcg cggctgtaat cctcaaggat atgcctgtcg
     4321 tattttccgg catcggtaag ttttttaacg tggtcgtaaa ggtgcggcgg ctcgtactcg
     4381 ccgtaggcta ttttacgaag atggaaaatc gccaaacgcg cggcaaggta ttggtagtcc
     4441 ggggtatctt ccgaaattaa atcggcagcg gctttgatga tggtttcgtg gatgtcgtcg
     4501 gtgcggatgc cgttgtagaa ctggatgtgc gatttcaact cgacctgaga tacggaaaca
     4561 ttttccaatc cgtccgccgc ccaagtgacg acacggtgaa tcttatccaa atcaatggct
     4621 tctaatcttc cgtctcgttt ggttactttc aaatcagtcg gtgtattcat cgcttcctct
     4681 tccactcttg atattcaaga cacagtcttt tcaaataaat taaggcagac aatatagtgg
     4741 atttttggca ttttctccag tcttgacaac ggttgtattt ttcagtttca ggcaatgcgc
     4801 gataaaaccc gcctgtcgta tttttcctat aatatttgtt ttatctgata attctttacc
     4861 gataaaaaac gggtaaattt tttgcctttt gaccggctcc ggctacaagg cggtgaaata
     4921 aggattttcc gacgaaaaag gaaagcttcc tgttttctgc ccctgcaaat tgttaaattt
     4981 tgcaaagtat gattttgccg cgccgccgcc gacaaattcc attttcttac cgattggaat
     5041 ttattattga gattaatgtg ttatttgaat ctgcatatca aacggcaagt tttgtcggct
     5101 gaatgagtct gaaactgccg acaatttgcc cgttccatcc ttccatccta tactggaaag
     5161 aatgacaaac tgaaaggatc gtcatgtctg tcaaaaagat gcttgccata ctgttgtctg
     5221 caatattggg actggtatcg acaactgccg ctgccggtac gtcagaaccc gcccaccgcg
     5281 ataccaaaca tatccgcaag gcaaacaagc agatgctgca ccccgaatgc aggaaatatt
     5341 tggaacgccg tgccgcgtgg taccgatcgc aaggcaacgt gcaggaattg cgcgaaaaca
     5401 aaaaggcgcg caaagcattc cgctccctgc cttatgcgga acagaaaatc caatgccggg
     5461 cggcttatga ggctttcgat gatttcgacg gcggcagttt ccgccgttaa tcccatataa
     5521 aatatgccgt ctgaacacag gttcagacgg cattttccat agatacaaca aactatcctt
     5581 acggcatttc gtttgaggtg ctcccgttgc cgcagcct
//
LOCUS       DAWFAD010000119         5596 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWFAD010000119 DAWFAD010000000
VERSION     DAWFAD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.119"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..381)
                     /locus_tag="WGO49_001620"
     CDS             complement(<1..381)
                     /locus_tag="WGO49_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
                     /protein_id="HEZ0306712.1"
                     /translation="MEDKQGMKKAVAGVMTDASSDGRKPATASNLPPLSNRGGNRGGT
                     ETETAGRVQEVFECYEKYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYG
                     VRVSLLEDEDFIRAASMLAEEVFGF"
     gene            483..698
                     /locus_tag="WGO49_001621"
                     /pseudo
     CDS             483..698
                     /locus_tag="WGO49_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(850..1908)
                     /gene="trpD"
                     /locus_tag="WGO49_001622"
     CDS             complement(850..1908)
                     /gene="trpD"
                     /locus_tag="WGO49_001622"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ0306713.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQVAQSIRQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLEGREGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(1973..2563)
                     /locus_tag="WGO49_001623"
     CDS             complement(1973..2563)
                     /locus_tag="WGO49_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ0306714.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVYHSGKGMFKGLPNPVTCTRYHSLVIERGTLPECLEITAWTEDGEIMGVRHKEY
                     AVEGVQFHPEALLTEYGHDMLNNFFIEFQNFKPQKI"
     gene            2985..5261
                     /locus_tag="WGO49_001624"
     CDS             2985..5261
                     /locus_tag="WGO49_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0306715.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKILSGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGELGLRLSSGNLEKLTSGGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSHADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDDAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVHLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSAISEAVKQPMLLDNKVQHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
BASE COUNT         1441 a         1567 c         1275 g         1313 t
ORIGIN      
        1 gaaaccgaaa acttcttcgg cgagcatgga cgcggcgcga ataaaatctt cgtcttccaa
       61 aagacttaca cgaacgccgt atttatcgaa aaaggttttt tcatgaaatg aaaagctaat
      121 ttgatcgatg aacgccgaat cagatacacc gcgacgaagc ggaacgccta aaaggtttcc
      181 tttgccgtct gttatgtact tttcataaca ctcgaagact tcttgaaccc tgcccgccgt
      241 ttcggtttct gtaccccccc tgttaccccc cctgttagat aaggggggaa gatttgaagc
      301 ggttgccggc ttcctgccgt ccgatgacgc gtccgtcatc acgccggcaa ccgccttttt
      361 catcccttgc ttatcttcca tggtgcgaat cctcaaaaac gggcaaaaaa aagccctgtt
      421 acttgtagaa agtaaaggac gttaattttt gttaatcgtc ccttcttagg gacgcaatat
      481 ataaggttta taccgtcgtt gttcctaatg cgcaatcagc gacattgttg ccaattatcc
      541 gaaagaaagt taagcctgat ggcattgtat acacggatac ctttcgtagt tatgatgtgc
      601 ttgatattag tgaatttagc catttacgta agtttaacgg cattcccaaa gagcatttgg
      661 ggctgcattt aaagaaatgc caatggcatt ttaaatagaa tctcaaattc agattttaag
      721 acaattagtt aatgggaatt tggtctggtt atctagtaca gccccaagtt tttatataaa
      781 aacagtttgg taagttctct tcgtgaagtg cttatgtttt gccacaaaag ttccataatc
      841 atggcggcat cacccaagct ccatctttcc aagaaaatgg catctttgtt gtggttgaaa
      901 accgacaaac tcctcttttt tcgatttggc cctgcctgaa tcgatggctt cccgtgcggc
      961 agatatgccg tctgaaagcg aagcagcgac atttccggca tacagggcgg cggcggtgtt
     1021 gagcaatacg atatcgcgcg cagccccttc tcttccttcc agcacctcat tcattttcaa
     1081 caaagattcc tgagtattgg caactttgat ttcatccaaa ttgcggcggg tttcgatacc
     1141 gaaatcttct gggcggatgt cgtattcgct gatttttccg tctttgagct cggcaacgcg
     1201 tgttttgccc gtcagtgtaa tttcatccaa accgccctcc ccgcaaacaa ccaaaacgtg
     1261 ttttgaacca agttgttgca agacccgcga caaaatgcca cacaaatcgg tgtggaacac
     1321 gcccaaaagc tggttcggcg cgcccgcagg attcgttaac ggacccaata tgttgaaaat
     1381 acttcggaaa ccgagcgaac ggcgtacagg ggcgacatgg cgcatggcac tgtggtgatt
     1441 gggcgcgaac ataaacccga tgccggtctg cctgatactt tgggcaacct gttcgggagt
     1501 caggttgagg tttgcgccca tctgctccac cacgtcagcc gcaccgctgg aggaagagac
     1561 cgaccggcct ccgtgtttgg caaccttcgc gcctgccgct gcggcaacaa acatcgaagt
     1621 cgtcgaaata ttgaaggttt tcgcgccatc cccgcccgta ccgacgatat cgaccagccc
     1681 ctctgcattc tccagcggca cttttgtcgc aaactcacgc atgacggctg cagctgcggt
     1741 aatttcggaa acggtttcaa ccttgatacg caatcctgtc aaaatggccg ctatctgctc
     1801 cggcagaacc tgtcctctca taatctgacg catcaagtcg gtcatttcat cgtaaaacaa
     1861 ctcgttattg ctgattaatc gttcgatggc ctgttgcggt gtaatcattt ttttgtcctc
     1921 cgttcaatat tcggacgaaa atgccgtctg aagggcttca gacggcatca cgtcagattt
     1981 tttgcggttt gaagttttga aattcgataa aaaaattgtt caacatatca tgtccgtatt
     2041 cggtcagcag agcttcaggg tggaattgta ccccctcaac ggcatattcc ttatggcgca
     2101 cgcccataat ctcgccgtct tccgtccatg ccgtgatttc caaacattcg ggcagcgtgc
     2161 cgcgttcgat aacgaggctg tgataacgcg tacaggtaac cggattgggc aaacccttaa
     2221 acataccctt gcccgaatgg tacacgggcg acaccttgcc gtgcatcagc gttttggcgc
     2281 ggactatcct gccgccgaac gcctcgccta tcgtctgatg cccgaggcac acgcccataa
     2341 tcggcagcct gccggcaaaa tggcgcatcg ccgccacgga aatccccgct tctttgggcg
     2401 aacacggacc ggggccgata acgagatatt gcggattcaa tgcctcgatt tcctccaacg
     2461 taatatcatc gttgcggcgc acggcaactt cctgccccaa ttcagtgaaa tactggacga
     2521 tgttgtaagt aaaactgtcg taattgtcga taaacaaaag cattttgtga ttaacctatt
     2581 gatttataat ttgtttgtta ttaactgcat ttccgatacc cccatccata ccctcttata
     2641 tcttagcgtg cccgatgcgc cctcgtgaac ctggcgcaga gcctcgcgtt ctgacaagta
     2701 cgccattttg ccaccttttc gaccgttcgg gaacgtgccg tttttatgca ctcataaatt
     2761 ttggttatcc cgcagccgaa cataatgggc aaacacagcg gaatctgcgt caacatcaac
     2821 cgcgcccccg aaggacggcg gatacaaatg gacaccgccc gatgccgcaa ccgtttcatt
     2881 tgaaaaactt ttgcctatgc cctctgagtg ttctcaaaat cttatgatat aaggaatgca
     2941 atgttatata ataacaaact tttgaaatgc gacgagaagc caaaatggca caaactacac
     3001 tcaaacccat tgttttatca attcttttaa tcaacacacc cctcctcgcc caagcgcatg
     3061 aaactgagca atcggtggat ttggaaacgg tcagcgtcgt cggcaaaagc cgtccgcgcg
     3121 ccacatccgg gctgttgcac acttcgaccg cttccgacaa aatcctctcc ggcgatacct
     3181 tgcgccaaaa agccgtcaac ttgggcgatg ctttggacgg cgtgccgggc attcacgcct
     3241 cgcaatacgg cggcggcgcg tccgctcccg ttattcgcgg tcaaacaggc aggcggatta
     3301 aagtgttgaa ccatcacggc gaaacgggcg atatggcgga tttttcgccc gatcacgcca
     3361 ttatggtaga taccgccttg tcgcaacagg tcgaaatcct gcgcgggccg gttacgctct
     3421 tgtacagctc gggcaatgtg gcggggctgg tcgatgttgc cgatggcaaa atccccgaaa
     3481 aaatgcctga aaacggcgta tcgggcgaac tcggattgcg tttgagcagc ggcaatctgg
     3541 aaaaactcac gtccggcggc atcaatatcg gtttgggcaa aaactttgta ttgcacacgg
     3601 aagggctgta ccgcaaatcg ggggattacg ccgtaccgcg ttaccgcaat ctgaaacgcc
     3661 tgcccgacag ccacgccgat tcgcaaacgg gcagcatcgg gctgtcttgg gttggcgaaa
     3721 aaggttttat cggcgtagcg tacagcgacc gtcgcgacca atatggtctg cctgcccaca
     3781 gccacgaata cgatgattgc cacgccgaca tcatctggca aaagagcttg attaacaaac
     3841 gctatttaca gctttatccg cacctgttga ccgaagaaga catcgattac gacaatccgg
     3901 gcttgagctg cggcttccac gacgacgatg atgcacacgc acacacccac agcggcagac
     3961 cgtggataga cctgcgcaac aaacgctacg aactccgtgc cgaatggaag caaccgttcc
     4021 ccggttttga agccctgcgc gtacacctga accgcaacga ctaccgccac gacgaaaaag
     4081 caggcgatgc agtcgaaaac ttttttaaca accaaacgca aaacgcccgc atcgagttgc
     4141 gccaccaacc cataggtcgt ctgaaaggca gctggggcgt gcaatattta caacaaaaat
     4201 ccagtgcttt atctgccata tccgaagcgg ttaaacaacc gatgctgctt gacaacaaag
     4261 tgcaacatta cagctttttc ggtgtagaac aggcaaactg ggacaacttc acgcttgaag
     4321 gcggcgtacg cgtggaaaaa caaaaagcct ccatccgcta cgacaaagca ttgattgatc
     4381 gggaaaacta ctacaaccat cccctgcccg acctcggcgc gcaccgccaa accgcccgct
     4441 cattcgcact ttcgggcaac tggtatttca cgccacaaca caaactcagc ctgaccgcct
     4501 cccatcagga acgcctgccg tcaacgcaag agctgtacgc acacggcaaa cacgtcgcca
     4561 ccaacacctt tgaagtcggc aacaaacacc tcaacaaaga gcgttccaac aatatcgaac
     4621 tcgcgctggg ctacgaaggc gaccgctggc aatacaatct ggcactctac cgcaaccgct
     4681 tcggcaacta catttacgcc caaaccttaa acgacggacg cggccccaaa tccatcgaag
     4741 acgacagcga aatgaagctc gtgcgctaca accaatccgg tgcggacttc tacggcgcag
     4801 aaggcgaaat ctacttcaaa ccgacaccgc gctaccgcat cggcgtttcc ggcgactatg
     4861 tacgaggccg tctgaaaaac ctgccgtccc tacccggcag ggaagatgcc tacggcaacc
     4921 gtcctttcat cgcacaggac gaccaaaatg cccctcgcgt tccggctgcg cgcctcggcg
     4981 tccacctgaa agcctcgctg accgaccgca tcgatgccaa tttagactac taccgcgtgt
     5041 tcgcccaaaa caaactcgcc cgctacgaaa cacgcacgcc cggacaccat atgctcaacc
     5101 tcggcgcaaa ctaccgccgc aatacgcgct atggcgagtg gaattggtac gtcaaagccg
     5161 acaacctgct caaccaatcc gtttacgccc acagcagctt tctctctgat acgccgcaaa
     5221 tgggccgcag ctttaccggc ggcgtgaacg tgaagtttta aaatcggaca ggcaaacaaa
     5281 aaaggttttc agacggcatt ctgaaaacct tttttctttt taatcaattt gttaggaaat
     5341 atggcaaaga caaagaacaa accactatgc catcattccc attttctttg tttcatcatt
     5401 cttgctttca cgccgtcatt ctcgcaaaag cagaaaaatc aaaagcagaa acccatcatt
     5461 cccgcgcagg cgagaatcca attcattcgg tttcagccgt ttccgataaa tcgccttagc
     5521 atttgggttt tagattccca ctttcgtggg aatgacggcg taaaggtttc tgttttttaa
     5581 gttttaggta actaat
//
LOCUS       DAWFAD010000120         5564 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWFAD010000120 DAWFAD010000000
VERSION     DAWFAD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.120"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(22..135)
                     /gene="rrf"
                     /locus_tag="WGO49_001625"
     rRNA            complement(22..135)
                     /gene="rrf"
                     /locus_tag="WGO49_001625"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(232..3123)
                     /locus_tag="WGO49_001626"
     rRNA            complement(232..3123)
                     /locus_tag="WGO49_001626"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3530..3605)
                     /locus_tag="WGO49_001627"
     tRNA            complement(3530..3605)
                     /locus_tag="WGO49_001627"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3570..3572),aa:Ala,seq:tgc)
     gene            complement(3611..3687)
                     /locus_tag="WGO49_001628"
     tRNA            complement(3611..3687)
                     /locus_tag="WGO49_001628"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3651..3653),aa:Ile,seq:gat)
     gene            complement(3788..5328)
                     /locus_tag="WGO49_001629"
     rRNA            complement(3788..5328)
                     /locus_tag="WGO49_001629"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1189 a         1619 c         1193 g         1563 t
ORIGIN      
        1 tcgggggttt tctgaaaggg tgtttggcag tgacctactt tcgcatggaa gaaccacact
       61 atcatcggcg ctgagtcgtt tcacggtcct gttcgggatg ggaaggcgtg ggaccaactc
      121 gctatggccg ccaaacttaa actgttacaa atcggtaaag ccttaatcaa tatattcggt
      181 aatgactgaa tcagtcagta agcttttatc tcttgaagtt cttcaaatga tagagtcaag
      241 cctcacgagc aattagtatg ggttagcttc acgcgttacc gcgcttccac accccaccta
      301 tcaacgtcct ggtctcgaac gactctttag tgcggttaaa ccgcaaggga agtctcatct
      361 tcaggcgagt ttcgcgctta gatgctttca gcgcttatct cttccgaact tagctacccg
      421 gctatgcaac tggcgttaca accggtacac cagaggttcg tccactccgg tcctctcgta
      481 ctaggagcag cccccgtcaa acttccaacg cccactgcag atagggacca aactgtctca
      541 cgacgtttta aacccagctc acgtaccact ttaaatggcg aacagccata cccttgggac
      601 cgactacagc cccaggatgt gatgagccga catcgaggtg ccaaactccg ccgtcgatat
      661 gaactcttgg gcggaatcag cctgttatcc ccggagtacc ttttatccgt tgagcgatgg
      721 cccttccata cagaaccacc ggatcactat gtcctgcttt cgcacctgct cgacttgtcg
      781 gtctcgcagt taagctacct tttgccattg cactatcagt ccgatttccg accggaccta
      841 ggtaaccttc gaactcctcc gttacgcttt gggaggagac cgccccagtc aaactgccta
      901 ccatgcacgg tccccgaccc ggatgacggg tctgggttag aacctcaaag acaccagggt
      961 ggtatttcaa ggacggctcc gcagagactg gcgtctctgc ttctaagcct cccacctatc
     1021 ctacacaagt gacttcaaag tccaatgcaa agctacagta aaggttcacg gggtctttcc
     1081 gtctagcagc gggtagattg catcttcaca accacttcaa cttcgctgag tctcaggagg
     1141 agacagtgtg gccatcgtta cgccattcgt gcgggtcgga acttacccga caaggaattt
     1201 cgctacctta ggaccgttat agttacggcc gccgtttact ggggcttcga tccgatgctc
     1261 tcacatcttc aattaacctt ccagcaccgg gcaggcgtca caccctatac gtccactttc
     1321 gtgttagcag agtgctgtgt ttttaataaa cagtcgcagc cacctattct ctgcgaccct
     1381 ccggggctta cggagcaagt ccttaacctt agagggcata ccttctcccg aagttacggt
     1441 atcaatttgc cgagttcctt ctcctgagtt ctctcaagcg ccttagaatt ctcatcctgc
     1501 ccacctgtgt cggtttgcgg tacggttcga ttcaaactga agcttagtgg cttttcctgg
     1561 aagcgtggta tcggttgctt cgtgtccgta gacactcgtc gtcacttctc ggtgttaaga
     1621 agacccggat ttgcctaagt cttccaccta ccggcttaaa caagctattc caacagcttg
     1681 ccaacctaac cttctccgtc cccacatcgc atttgaatca agtacaggaa tattaacctg
     1741 tttcccatcg actacgcatt tctgcctcgc cttaggggcc gactcaccct acgccgatga
     1801 acgttgcgca ggaaaccttg ggctttcggc gagcgggctt ttcacccgct ttatcgctac
     1861 tcatgtcaac attcgcactt ctgatacctc cagcacactt tacaatgcac cttcatcggc
     1921 ctacagaacg ctcccctacc atgccgataa accggcatcc gcagcttcgg ttatagattt
     1981 gagccccgtt acatcttccg cgcaggacga ctcgaccagt gagctattac gctttcttta
     2041 aatgatggct gcttctaagc caacatcctg gctgtctggg ccttcccact tcgtttacca
     2101 cttaatctat cattggggac cttagctggc ggtctgggtt gtttccctct tgacaacgga
     2161 cgttagcacc cgctgtctgt ctcccgagga accacttgat ggtattctga gtttgccatg
     2221 ggttggtaag ttgcaataac cccctagcca taacagtgct ttacccccat cagtgtcttg
     2281 ctcgaggcac tacctaaata gttttcgggg agaaccagct atctccgagt ttgtttagcc
     2341 tttcacccct atccacagct catccccgca ttttgcaaca tgcgtgggtt cggtcctcca
     2401 gtacctgtta cggcaccttc aacctggcca tggatagatc actcggtttc gggtctacac
     2461 ccagcaactc atcgccctat taagactcgg tttccctacg cctcccctat tcggttaagc
     2521 tcgctactga atgtaagtcg ttgacccatt atacaaaagg tacgcagtca cacctctagg
     2581 gcgctcccac tgtttgtatg catcaggttt caggttctgt ttcactcccc tcccggggtt
     2641 cttttcgcct ttccctcacg gtactggttc actatcggtc gatgatgagt atttagcctt
     2701 ggaggatggt ccccccatat tcagacagga tttcacgtgt cccgccctac ttttcgtacg
     2761 cttagtaccg ctgttgagat ttcgaatacg ggactgtcac ccactatggt caagcttccc
     2821 agcttgttct tctatctcga cagttattac gtacaggctc ctccgcgttc gctcgccact
     2881 acttgcggaa tctcggttga tttcttttcc tccgggtact tagatggttc agttctccgg
     2941 gttcgcttct ctaaatctat gtattcaact taggatactg cacagaatgc agtgggtttc
     3001 cccattcgga catcgcggaa tcattgcttt attgccggct cccccgcgct tttcgcaggc
     3061 ttacacgtcc ttcgtcgcct atcatcgcca aggcatccgc ctgatgcact tattcacttg
     3121 actctatcat ttcaagaact tctttgactt tgcctaacat tccgttgact agaacatcag
     3181 acttgaattt cctactttga taaagcttac tgctttgttg tgtcttaatc ctgccttttg
     3241 tgtttcagga ttaagtcgat acaatcatca cccaaatact gtgtttgttt tcttttctct
     3301 tgtgagcgat tttttatcct ttgcaaagaa taaaaaatca aaacaaacgc tttgtctttg
     3361 tttgttgatt tcggctttcc aatttgttaa agatcgatgc gttcgatatt gctatctact
     3421 gtgcaaatca aaacgagctg attattatat cagcattttg ttcttggtca agtgtgatgt
     3481 cgccctgaat ggattctgtt ccattcttcc gttttgattt gtacagtatt ggtggaggca
     3541 aacgggatcg aaccgatgac cccctgcttg caaagcaggt gctctaccaa ctgagctatg
     3601 cccccgttct tggtgggtct gggaggactt gaacctccga ccccacgctt atcaagcgtg
     3661 tgctctaacc agctgagcta caaacccgga ttctcttctt aagcgaatct tgccttcact
     3721 caagcttctt ccgcatcttt tcagtttacc gataagtgtg aatgcctaaa gcctcttctt
     3781 tctctagaaa ggaggtgatc cagccgcagg ttcccctacg gctaccttgt tacgacttca
     3841 ccccagtcat gaagcatacc gtggtaagcg gactccttgc ggttacccta cctacttctg
     3901 gtatccccca ctcccatggt gtgacgggcg gtgtgtacaa gacccgggaa cgtattcacc
     3961 gcagtatgct gacctgcgat tactagcgat tccgacttca tgcactcgag ttgcagagtg
     4021 caatccggac tacgatcggt tttgtgagat tggctccgcc tcgcggcttg gctaccctct
     4081 gtaccgacca ttgtatgacg tgtgaagccc tggtcataag ggccatgagg acttgacgtc
     4141 atccccacct tcctccggct tgtcaccggc agtctcatta gagtgcccaa ctgaatgatg
     4201 gcaactaatg acaagggttg cgctcgttgc gggacttaac ccaacatctc acgacacgag
     4261 ctgacgacag ccatgcagca cctgtgttac ggctcccgaa ggcactcctc cgtctctgga
     4321 ggattccgta catgtcaaga ccaggtaagg ttcttcgcgt tgcatcgaat taatccacat
     4381 catccaccgc ttgtgcgggt ccccgtcaat tcctttgagt tttaatcttg cgaccgtact
     4441 ccccaggcgg tcaatttcac gcgttagcta cgctactaag caatcaagtt gcccaacagc
     4501 taattgacat cgtttagggc gtggactacc agggtatcta atcctgtttg ctacccacgc
     4561 tttcgggcat gaacgtcagt gttgtcccag gaggctgcct tcgccatcgg tattcctcca
     4621 catctctacg catttcactg ctacacgtgg aattctacct ccctctgaca cactcgagtc
     4681 acccagttca gaacgcagtt cccgggttga gcccggggat ttcacatcct gcttaagtaa
     4741 ccgtctgcgc ccgctttacg cccagtaatt ccgattaacg ctcgcaccct acgtattacc
     4801 gcggctgctg gcacgtagtt agccggtgct tattcttcag gtaccgtcat cagccgctga
     4861 tattagcaac agccttttct tccctgacaa aagtccttta caacccgaag gccttcttca
     4921 gacacgcggc atggctggat caggcttgcg cccattgtcc aaaattcccc actgctgcct
     4981 cccgtaggag tctgggccgt gtctcagtcc cagtgtggcg gatcatcctc tcagacccgc
     5041 tactgatcgt cgccttggta ggcctttacc ccaccaacta gctaatcaga tatcggccgc
     5101 tcgaatagcg caaggcccga aagtcccctg ctttctctct caagacgtat gcggtattag
     5161 ctgatctttc gatcagttat cccccactac tcggtacgtt ccgatatgtt actcacccgt
     5221 tcgccactcg ccacccgaga agcaagcttc tctgtgctgc cgtccgactt gcatgtgtaa
     5281 agcatgccgc cagcgttcaa tctgagccag gatcaaactc ttatgttcaa tctctaactt
     5341 tttaacttct ggtctgcttc aaagaaacca acaggacaat gttcaaaaca ttatcttgtc
     5401 tgtctttcaa acagtgtgag gctcaaggca ctcacactta tcggtaatct gttttgttaa
     5461 agagcgtttg aattataaag tatttcttcc gcctgtcaag atatctctcg atatccccaa
     5521 cattctgtgc tatacttttc agttcgtccg ccgcttctgc agca
//
LOCUS       DAWFAD010000121         5500 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWFAD010000121 DAWFAD010000000
VERSION     DAWFAD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.121"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..151)
                     /locus_tag="WGO49_001630"
     CDS             complement(<1..151)
                     /locus_tag="WGO49_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ0306716.1"
                     /translation="MLKTLTFAALHFSTAFSITYVLTGSIGVSGAVALVEPLINTVVF
                     YSGLTK"
     gene            273..1226
                     /locus_tag="WGO49_001631"
     CDS             273..1226
                     /locus_tag="WGO49_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0306717.1"
                     /translation="MNQKKVVVLDADTLPGRTFHFDFPHELAVYGTTDAAETAGRVRD
                     AHIIITNKVMISADIIAHAPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(1304..3358)
                     /gene="metG"
                     /locus_tag="WGO49_001632"
     CDS             complement(1304..3358)
                     /gene="metG"
                     /locus_tag="WGO49_001632"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ0306718.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTDFGIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQAE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFESRLKDVADSALLAKLTAQSEAIAECYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDARLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AARFLNLDAITWANTRVTLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIQTTPAPVE
                     DSKYEKVAEQAGFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIAA
                     FYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGDK
                     VG"
     gene            complement(3464..5302)
                     /gene="glmS"
                     /locus_tag="WGO49_001633"
     CDS             complement(3464..5302)
                     /gene="glmS"
                     /locus_tag="WGO49_001633"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ0306719.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGNIK
                     RVRRVGRVQLMEDAAREKHLNGSLGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEDLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPVVHTIPVQLLSYHVALARGTDVDKPRNLAKSVTVE"
BASE COUNT         1116 a         1332 c         1676 g         1376 t
ORIGIN      
        1 tttttgttaa tccactatag aaaacgacgg tgttgattag aggctcgacc agcgcgaccg
       61 cccccgatac gcctatactg cccgtcagta cataggttat actgaaggcg gtgctgaaat
      121 gcagtgcggc aaaagtcagg gttttaagca tcatcatctc tcggattgga cattgacgga
      181 gggatgataa agattatcat aaggctgtgc ggtttaaatt tgctatttgc tgttagtgta
      241 aataaatcat tttctaaata gggataaaaa ttatgaatca gaagaaagta gtcgtattgg
      301 atgcggatac tttacccggt cggacttttc acttcgattt cccacacgag cttgcggttt
      361 acggtacgac agatgctgcg gaaacggcag gacgggtgcg cgacgcgcat attatcatca
      421 ccaataaagt gatgatttca gcggatatca ttgcacacgc tccgcaactt gaattgattg
      481 ccgtcagtgc gaccggtgtg aacaatgtcg atattggggc ggcgaaggcg gcgggcgttg
      541 cggtatgcaa tgtccgcgcc tacggaaacg aatcggttgc ggaacacgcc tttatgctga
      601 tgattgcgtt gatgcggaat ttgcctgcct atcagcgtga tgttgcggca ggattatggg
      661 agaagtcgcc gtttttctgc cattacggcg cgccgattcg ggatttgaac ggtaaaacgc
      721 tggcggtttt cggacgtggc aatatcggac ggacgcttgc cggatacgcg caggcattcg
      781 gtatgggggt ggtgtttgcc gaacacaaac acgcgcccga cgtgcgcgaa ggctatgttt
      841 cctttgaaga tgcggtacag actgccgacg tgttgtcgct gcactgtccg ctaaacgccc
      901 aaactgaaaa tatgataggc gaaaacgaat tgcggcagat gaagcctggc gcggttttaa
      961 tcaattgtgg gcgcggcggg ctggtggatg aaaacgcgct gcttgccgca ctcaaatacg
     1021 ggcagatcgg tggggcgggt gtcgatgttt tgacggagga gccgccaagg ggcggcaatc
     1081 ccttgctgaa tgcacgcctg cccaatctga ttgttacgcc gcataccgcg tgggcgagcc
     1141 gcgaggcttt ggacaggctg tttgatatat tgttggcgaa cattcacgcc tttgtgaaag
     1201 gcgaggcaca aaatcgggtg gtttgagctt gtcgggatta tgggaaaaca atgccgtctg
     1261 aaggtctcaa gggtccagac ggcattgttt ttgatatttc tacttaaccg actttgtcgc
     1321 ccggttgcgc gcctgcgtcg acatcaagca gtttcagttt cccgtctgcc gtggcggcac
     1381 tcaaaatcat gccttcggat acgccgaatt tcgccatttt gcgcggggcg aagttggcga
     1441 cggcaatgac cattcttccg tttaactcgg cagggttggg gtaaaacgcg gcgatgccgg
     1501 agaagatgat acgtttttca aaaccgaaat cgaggtcgaa tttcaggagt ttggtgctgc
     1561 cttcgacggc ttcgcagttc aatactttgg cgacgcgcat atcgattttc ataaagtcgt
     1621 cgaaacccgc ctgttcggcg actttttcgt atttgctgtc ttcaacaggc gcgggggttg
     1681 tctgaatact ttgtttgttg gcttcgatta aatcgtccac ttgtttttgc tccactcgtt
     1741 gcattaaatg ttcgtatttg ttgatggcgt gttcgcccaa ggttaccctt gtgttcgccc
     1801 aagtaatcgc gtccagattg aggaaacgcg cggcgttggc ggcggtttgc ggcaacacgg
     1861 gggcgaggta ggcggtcaac atggtgaagg cgttgatgag ctcgctgcat acttcgtgca
     1921 ggcgtgcgtc ttggccttct tgtttcgcca gttcccaagg tttgttggca tcaacatatt
     1981 cgttgacaat gtctgccaat gccatgatgt cgcgcagggc tttggcgtat tcgcggcttt
     2041 cgtagcattc ggcaatggct tcgctttgcg cagtcagttt tgccagcaac gcgctgtcgg
     2101 caacgtcttt cagacggctt tcaaagcgtt tggcgataaa acctgacgcg cgggcggcga
     2161 tgttgacgta tttgcctacg aggtcgctgt tgacgcgcga gataaagtct tgcaggttca
     2221 aatcgatgtc ttcgattttg ctgttgagtt tggcggcgat gtagtagcgc atccactcgg
     2281 ggtttaaacc ttgttccaga taggatttag cggtgatgaa ggtgccgcgc gatttggaca
     2341 ttttttgtcc gtccacggtc aaaaagccgt gtgcgtacac gccggtcggg gcgcggtggc
     2401 cggagaagtg cagcatggca ggccagaaca gggcgtggaa atagaggatg tctttgccga
     2461 tgaagtggta catctcggtt tggctgtcgg ctttgaagta ttcgtcaaaa tcgacgccga
     2521 tgcggtcgca caggttttta aacgacgcca tgtagccgac gggcgcgtcc agccagacgt
     2581 agaagtattt gcccggcgcg tcggggattt caaagccgaa atacggcgcg tcgcgggaaa
     2641 tgtcccagtc ggacagcgtg gtttcttcgc cttcgcccag ccattctttc attttgttga
     2701 gggcttcggc ttgcagatgg ggcttgccgt cgtgcgggtt gttgccggaa gtccatgctt
     2761 tgaggaagtc ggcgcattcg cccagtttga agaagaagtg ttcggattcg cgcaattcgg
     2821 gtttcgcgcc ggaaacggcg gaatacgggt tgatcagttc ggtcggggaa taggtcgtgc
     2881 cgcagacttc gcagttgtcg ccgtattggt cttgggcgtg gcatttcggg cattcgcctt
     2941 tgacgaagcg gtcgggcagg aacatttgtt tttcggggtc gaaaagctgc tcgatgacgc
     3001 ggctctcaat cttgccgttg gctttcagcg cgcggtaaat gtcttgggaa aactgtttgt
     3061 tttcagggga atgggtgctg taataattgt cgtaaccgat gccgaaatcg gtaaagtcgg
     3121 cgaggtgctc ttcgcgcact ttggcaatca tgtcttcggg cgcgatgcct tgtttttgcg
     3181 cggcaagcat tacgggcgtg ccgtgagtgt cgtcggcgca gcagtagtag cattcgtggc
     3241 cgcgcagttt ttgaaagcgc acccaaacgt cggtttggat gtgttcgacc atatggccga
     3301 ggtggatgct gccgttggca tagggcaggg cggaggtaac gaggattttg cgtgtcatgg
     3361 tttatgcttt tggggataga tggtaaaggg gaattatacc gtaaatgccg tctgaagcct
     3421 gaaaaatcag gcttcagacg gcatgttcgc aaacaatccg gacttattcg accgtaacgg
     3481 atttcgccag gttgcgcggt ttgtctacat cggtgccgcg tgcgagggcg acatggtagg
     3541 aaaggagctg cacggggata gtatgcacga cgggggacag tttgccgacg tggcgcggtg
     3601 cgcggataac gtgcacacct tcggtggcat taaaattgct gtcgaggtcg gcaaagacga
     3661 aaagttcgcc gccgcgcgcg ccgacttcct gcatattggc tttgactttg tccaacaggc
     3721 tgtcgttggg tgcgatgacg acgacgggca tgttttcgtc caccaaggca agcggcccgt
     3781 gcttcagttc gccggcagga taggcttcgg cgtggatgta ggtgatttcc ttcagtttta
     3841 acgcgccttc gagggcaatc gggtaatgga tgccgcgccc caaaaacagc gcgctggttt
     3901 tcttggcaaa ctgttgcgcc caggcggcaa tttgcggttc gaggtttaaa gcgtgctgca
     3961 cgctgccggg aagctggcgg agttcttcgg tgtaacgtgc ttcgtcttct tcggaaacca
     4021 aaccgcgcac tttcgccagc gttaccgcca aaccgaacag cgcaaccagc tgcgtggtaa
     4081 atgctttggt tgaggcgacg ccgatttccg caccggcacg ggtataaagc acgaggctgc
     4141 tttcgcgcgg cagggcggat tccatcacgt tgcagatgga gaggctgtgg cggtgtccca
     4201 aggattttgc gtatttcaac gcctccatcg tgtccagcgt ttcgccggat tgggaaatgg
     4261 taatgaccag ttggtccgga tcggcaatca cgctgcggta tcggtattcg ctggcgattt
     4321 cgacatcggc cgggattttg gcgatggatt ccaaccaata tttggcggtc agcgcggcgt
     4381 aataggacgt gccgcaggca aggattttga cgctgcggat gctttcaaac acgcttttgg
     4441 cgtttttgcc gaagttttcg gggatgaagc cgccgtcgag gaaaacctcc gccgtgtctg
     4501 caatcgcgcg cggctgctca tggatttcct tttgcataaa gtggctgtac ggtcccagtt
     4561 ccaaagaggc gagcgagagt tcggatacct tgactttgcg ttcggcaggc aggccgtttt
     4621 tatcggtcag ccttttgatg ccgtctgaag ccagcagcgc gatgtcgccg tcttcgaggt
     4681 acgccacgcg gcgcgtaaag gcgatgacgg cggatacgtc cgaagcgata aaggtttcat
     4741 cgtcgcccaa agcgaccaaa agcgggcagc ccatacgcgc cacgactaat tcatcaggct
     4801 tgtcttgggc aataaccgcg atggcgtatg cgccgtggaa acgtttgacc gcttcctgca
     4861 cggcttcaaa cagcctgccg ccgttttgcg cgtattcgtg attgatgctg tgggcgatga
     4921 cttcggtatc cgtttgcgat tcaaaacggt atcccaaatc ttccaaacgt ttgcgttcgc
     4981 tttcaaagtt ttcgatgatg ccgttgtgca cgaccgcaat cataccgccg ctgatgtgcg
     5041 ggtgggcgtt cggctcggta acgccgccgt gtgtcgccca gcgcgtatgc ccgatgccca
     5101 aactgccgtt caaatgcttt tcacgcgccg catcttccat taactgcacg cgcccgacac
     5161 ggcgcacgcg cttgatgttg ccgtcggtgt tgacggcaat gcctgatgaa tcataacccc
     5221 ggtattcgag gcgtttgaga ccgtcggtca gaaaatcgac gacattgtga tgggcgcgga
     5281 tggcgccgac gataccgcac ataactgttc cttagtatcc ggttgaaaaa aaaacaggcg
     5341 cggacggctc ccgtgccgca ccttccgctt cggattataa accgcctccc gcgccggaaa
     5401 acagcaaaat gccgtctgaa ggcttgggct tgctcaaaaa agggagggat ttccccgcct
     5461 gaccgggatg ggcgttcaga cggcattgcc tgctgccggt
//
LOCUS       DAWFAD010000122         5457 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWFAD010000122 DAWFAD010000000
VERSION     DAWFAD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.122"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..891)
                     /gene="mafA"
                     /locus_tag="WGO49_001634"
     CDS             complement(<1..891)
                     /gene="mafA"
                     /locus_tag="WGO49_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0306720.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVE"
     gene            complement(1075..1794)
                     /locus_tag="WGO49_001635"
     CDS             complement(1075..1794)
                     /locus_tag="WGO49_001635"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ0306721.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(2011..2865)
                     /locus_tag="WGO49_001636"
     CDS             complement(2011..2865)
                     /locus_tag="WGO49_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ0306722.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDVGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(2994..3722)
                     /gene="rpsB"
                     /locus_tag="WGO49_001637"
     CDS             complement(2994..3722)
                     /gene="rpsB"
                     /locus_tag="WGO49_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ0306723.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDHRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(3923..4132)
                     /locus_tag="WGO49_001638"
     CDS             complement(3923..4132)
                     /locus_tag="WGO49_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306724.1"
                     /translation="MWHIVAIGYLFVAVMFSAAQPSIARTLIYLVFWAVLPTVFTVFV
                     VTIRRRNRLMKQQEQAEFEQQRAQR"
     gene            4210..4791
                     /locus_tag="WGO49_001639"
     CDS             4210..4791
                     /locus_tag="WGO49_001639"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ0306725.1"
                     /translation="MRNEEKHALRRELRGRRSQMGWDVRAAATVKINRLLKRYIKHGR
                     KIGVYWPMGKELRLDGFVRAAQKRGAKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            4852..5442
                     /locus_tag="WGO49_001640"
     CDS             4852..5442
                     /locus_tag="WGO49_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0306726.1"
                     /translation="MSERIILPVLSLGGVRLEPLDVHHEMGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         1220 a         1243 c         1526 g         1468 t
ORIGIN      
        1 ctctacggat ggggcggagt tacccgtatg attgccgtat ggtcggatat cggagaaatc
       61 gaccattaat ccttccgtcg gtttaattcc tttgcttact ttatacggcc ccatccacaa
      121 tgcgtaattt tctttatagg cagcttcaaa cgcattggtt ttgggtttga tgagcaattt
      181 tttattggtt ctgtctactg cgaaatattc cagttttgtt tgggctttca gtgtttcggc
      241 attgtatagg tgcatttcgg ttctgttgcg tatcgttccg aatacgtcga tgttaataaa
      301 cacatctgta tcggcattgg caggagaaac aacgtctatg ccgcgcagga aaaatacggt
      361 ctgtaccaag tgggaaagaa aggcagtgtc gcgcgggtta gtcgtcaagg tttcatttcg
      421 ataatccccc atcccgccaa tatttaagcc cagactgctt ttacttccgc taccgtctga
      481 ttgggtgcga gagagtgcag gggcattaag tgtagataaa gaagtggtta aacctgtcaa
      541 accgcctgat gttgtttcag cggtggtttc gtaacgtgga taggtgtaat cggtacggac
      601 ggcagggctg tttatgtatt cgccacgaat cagtgcatca atggagtagc gaccccctgt
      661 caaactgcct gaaccttggt cgcccatagt tgcaatgtac aatgcaactt ttcgtccgtg
      721 taatgcctgt aaatccatgt ctttaacggc agctctggca gaagcggcca caagttcttg
      781 ttcgaccgca aagcgtttac ctccgccatg cgatggaata cctgtcagtg tcccgcaggc
      841 ggataaaata aaaactgaaa aaagaatagg tatcagcagc cgtgcttgca tagattttct
      901 cctttgatga aaaacaaatt gtatcaaaat tgtaaatata gtggattaaa tttaaaccag
      961 tacggcaagg cgaggcaacg ccgtactggt ttaaatttaa tccactataa tctccatata
     1021 ataaatggct gtctgaagcc catatgcgac tatatctgcc gacactatgg tcaatcagca
     1081 gtgaaccagc gtaccttcgt tttcaccggt tactacacgt ttgagcgagc cttctttagc
     1141 aatgccgaat acgacaatat tgagcttgcg ttcgcggcag agggcgaaag cggtggcatc
     1201 catcactttg aggtttttca acaaggcttc gtcaaaagta atggtttcgt agcgcgtggc
     1261 ggatgagtct tttttggggt ctgcggtgta cacgccgtca acgttggtgg ctttgagcat
     1321 cacgtcgcag ttcatttccg caccgcgcaa tgcggcggca gtgtcggtcg tgaagaacgg
     1381 gttaccggta ccggcggcaa aaatcacgac tttgccttct tccaaatatt ggatggcttt
     1441 ggggcgggcg taggtttcag cgatttgctg catagacagt gcggattgta cgcgcgcttt
     1501 gatgcctaaa gtttcaaatg cgtctttgag tgccaacgcg ttcatcacgg tcgccatcat
     1561 gcccatgtag tcggcggtgg cgcgatccat gctgcctgct tgggcggata cgccccggaa
     1621 aatattgccg ccgccgacaa caataccgac ttgcacgccc attttaacga cttcggcaat
     1681 ttcgccgaca gtttgaacga tggtatcgtg attgatgccg aacggatcgg aacccatcag
     1741 ggattcgccg gagagtttca gtaatacgcg tttgtatttg atttgctgtg tcatggatac
     1801 cttgctttct tgaatgtcgt cggtcggagt tgggtcgtct gaatttcaaa cggtcggaat
     1861 attaatgata gggtgtgtcg gtttattatt gttttgtttt aagacggtct gaatattcgg
     1921 tcgtcttaaa ataggtaccg caaacggttt tctcaaaaaa aagcaccctg attcgtttgg
     1981 aatccaggtg ctttcttttt cataagtgcc ttacactttg gcggctgcgg ctacttcggc
     2041 tgcgtagtcg acaacggctt tctcgatacc gtcgcctact ttgtagcgga cgaagctgac
     2101 cacttcagtg ccgttttctt tggcgaattg ggcaacagtt tgatcggggt tcatcacgaa
     2161 tgcttggccg ttcagagtga tttcagccaa gaatttgcga atacggcctt caaccatttt
     2221 agcggcgatg tcggcaggtt tgccggaagc gatggcttgt tcggtgtaga tgtggcgttc
     2281 tttttcaacg gtttcggcat ctacttcggc ttcgcttacg cattgtggtt tagcggcaac
     2341 aatgtgcata ccgattttgc gtgctacgtc ttcagagcct ttgtactcaa ccaatacgcc
     2401 ttcggtcgcc aatgcgccgt ggatgtaggc aaccagttgg ttggcagtgt cgatcacttg
     2461 gaagcgacgg acagacatgt tctcgcccaa tttggcgatg atggctttgc gttctgcttc
     2521 aaccagttcg ctcagttctt caacagaagc cggttttttc tcggcggcag ttttcgcaac
     2581 gaagttggca aattctacga agcccacgtc tttagcaacg aagtcggttt cgcagtttac
     2641 ttcgaccaat gcgccgacat tgccgttgat cgcgtaagcc aatacgcctt cggcagcagt
     2701 acggccagcc agtttaccgg ctttcgcacc agatttaata cgcaggattt cttcggcttt
     2761 gtcgaagttg ccttcggctt caaccaaggc ttttttgcat tccatcatgc ccaggccggt
     2821 agcagcgcgc aggtcggcaa ccatttttgc agtaatttct gccattttga atctcctaga
     2881 tttttgggaa atacggaaag gattttccgc attcgggaat cgtgtgccgc gaacggacgt
     2941 tcagacggca tatttgagaa aaggggcata acgcccctct tcggtttgcc ggattactcg
     3001 gcagcggctt cttgggcagc ggctacggtt tcttgcagcg cttggttttt gccttccaaa
     3061 actgcgtcag cgatgccgcg gcagtacagg cggatggctt tggcggagtc gtcgttgccg
     3121 gggatgacgt atttcacgcc gtcagggctg ttgttggtat cgactacggc gataacgggg
     3181 atgcccagtt tttcagcctc gaccagagta cctttttggt agccggtatc gataacgaaa
     3241 atcgcgtcgg gcaggccttt catgtttttg ataccgccca aagaacgttc cagtttttca
     3301 acatcgcgtt gcatttccag aatttctttt ttgctgaaac cgctttcggc agcattttcc
     3361 aaggctgcgg ttttttcttc caggcgtttg atggattgct taacggtttt gtagttggtc
     3421 agcataccgc ccaaccaacg gtgatcgacg aaaggcatgc cggcgcgggt cgcttcttcg
     3481 cggatgatgt cgcgggcttg gcgtttggta cctacgaaca atactgtacc tttgttggca
     3541 accagacgac gtacggcttc ttgcgcgtct tggaacatcg gcagggtttt ttccaggttg
     3601 acgatatgga ttttgttgcg cgcaccgaaa atgtattgag ccattttcgg gttccagaaa
     3661 cgggtttggt ggccgaagtg gacaccggct tcaatcatct gacgcatagt aatttgagac
     3721 attttgattt ccttgaaagg gttaagggca cacattcaat cgcatagaac ttgatagacc
     3781 gcgcctattg tcaagcaccc ttcggcgaaa gtgggcataa gttttaaaaa ataaaagagt
     3841 gtttccgaaa tggaaaactg acggattata gcggataaga ggtcgtctga aaagggattc
     3901 agggttttgt gccgctgtct ttttaccgtt gtgcacgctg ctgttcgaat tctgcctgtt
     3961 cctgttgctt catcaggcgg ttgcggcggc ggatggtcac gacaaagacc gtgaacacgg
     4021 tgggcagcac cgcccaaaaa accaaataaa tcaaggtgcg cgcaatactc ggctgcgcgg
     4081 cggaaaacat cacggcaaca aaaagatagc cgatggcgac aatgtgccac attgtgtgcg
     4141 tcctgtttga atgttaacaa aatcgttata atcgcaacat tctaccgcaa tcctgaaagg
     4201 ccgtctgaaa tgaggaacga ggaaaaacac gccctgcgcc gcgaattacg cgggcggcgt
     4261 tcgcaaatgg ggtgggacgt gcgggcggcg gcaacggtaa aaattaaccg cctgctcaaa
     4321 cgttatatca agcacggtcg gaaaatcggc gtgtattggc cgatgggcaa ggaattgcgt
     4381 ttggacggct ttgtccgcgc ggcgcaaaaa cgcggcgcaa aactttatct gccttatatc
     4441 gaaccgcgtt cgcggcggat gtggtttacg ccgtatcctg aaagcggaat ggaacgggag
     4501 cgcatacggg gcagggcgag gttgaacgtg ccgcagtttg cagggcgcaa aatccgcgtg
     4561 cacggtttgt cggtattgct cgttccgctt gtcggcatag accgcgaagg ctaccgtttg
     4621 gggcaggcag gcggctatta cgatgcgacg cttgcagcga tgaaataccg tttgcaggca
     4681 aaaaccgtgg gcgtgggctt tgcctgccag ttggtggaca gactgtcggt cgaggcgcac
     4741 gacctgccgc tggacggttt tgtgtcggag gcggggatat tgtgttttta gccgttcaga
     4801 cggcattttg aacggaaatg ccgtctgaag gcggaatatt gggagaaaag gatgtcggaa
     4861 aggataatac tgccggtctt gtcgttgggc ggcgttcgtc tcgaaccgtt ggatgtgcat
     4921 catgaaatgg gtttgcgcga ggcggtttgc gatggggaag tttggaagct gggagtaacg
     4981 tccgcgcccc atccggatag ggtggcagac tatatcggga cagcattggc aacgcgtttg
     5041 gcgtttgcgg ttgtcgatga agaggcgggc agggtggtcg ggacaacggc gtattatcat
     5101 tttgaaccgc agattccgcg tttggatatc ggattcacat ggtatgcggc atcggcacgg
     5161 cggacacgga taaacacctg ttgcaaaatc atgctgttgg attacgcatt tgatgttttg
     5221 gcttgccgtt gcgtgggatg gcgcacggat attctgaacc ttgcctcgca acgtgccatc
     5281 gaacggctgg gtgcggaaaa agacggcgtg ctgcgtatgc atatgctccg taaagacggc
     5341 agtgtgcgcg atacggtcgt gtacagtatg ctgagggaag attggtgcaa aaacagggaa
     5401 atcctgaccg gcaggttggc agggtatggc gtgcaggtgt gaggcagtat tttcaaa
//
LOCUS       DAWFAD010000123         5456 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWFAD010000123 DAWFAD010000000
VERSION     DAWFAD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5456
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.123"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(145..1941)
                     /locus_tag="WGO49_001641"
     CDS             complement(145..1941)
                     /locus_tag="WGO49_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ0306727.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPAHWQARR
                     ELSGFTGSVGTFVVTADEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPNDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            2399..3652
                     /locus_tag="WGO49_001642"
     CDS             2399..3652
                     /locus_tag="WGO49_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0306728.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3807..5318
                     /gene="lysS"
                     /locus_tag="WGO49_001643"
     CDS             3807..5318
                     /gene="lysS"
                     /locus_tag="WGO49_001643"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ0306729.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSDIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
BASE COUNT         1179 a         1384 c         1605 g         1288 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat atttgcttgc
       61 ggcaagatga aaacggtgcg ggatgttttg ggaaaccaat gccgtctgaa gggctttcag
      121 acggcatttt ttgcgccgtg ctgtttaacg cgccagcggt tcggtgcgtt tgatcagcca
      181 cgcttttgcc gcgccttcgg tcagcggctc gaggcggcgg cggacttcgg cgtggtagcg
      241 gttgacccag tcgatttcgc cgtcggtcat gagggcggtg tccatcaggc gggtgtcgat
      301 ggggcagagg gtcagggttt caaaacagag gaagctgccg aattcggttt cttgaggggc
      361 ggcgacggct tggttggcgg caaggttttc aatgcggatg ccccattttc ccgggcggta
      421 gagtccgggt tcgatggagg taaccatgcc ttttttcatg gcggtttcgg gcgtggcggg
      481 ggcggcgaag gcgatgcgct gcgggccttc gtggacgttg aggaaatagc ctacgccgtg
      541 tccggtgccg tggccgtagt cgcattgcgc ctgccacagg ggtttgcggc aaatcgcatc
      601 aatcagcggc gaggggatgt tttctgggaa cacggcttcg gcaagcgcga tatgggcttt
      661 gagaacgagg gtgttgtcgc gtttttgttc ggcggtgggt gtgccgacag ggacgacgcg
      721 ggtgatgtcg gtcgtgccgc ctttgtattg cgcgccggag tcgatgagca aaagcccgtt
      781 gccgctgatg gtgctgtggc tttcgggcgt tgcgctgtaa tgcggcagtg cgccgttggc
      841 gttgaagcct gcgatggtgt cgaaactcaa tgaaatgaag cctgggcgca cgctgcggtg
      901 gcgataaagc atggtgtcca cgtcgatttc ggtcaggctg ccgccgttgc cgatgatgtc
      961 ttcaaactcg gcgaagaaac cgcacaacgc cgcgccgtcg tgttccatcg cttcgcggat
     1021 gcgggcgatg tcggcttcgg atttgcagga tttgaacagc gtggacgggt tgattccctc
     1081 gataaggcgc acgctttcgg gcaggcgcac aagcgtgctg acggcggttt tgttcggttc
     1141 gataagcaac gcgccgccga tttgcgcgag tttgtcggca acttgggcgt aaggctcgac
     1201 cgtgatgcct gcggtttgca gcgcggcggc ggcttcggcg ttcagacggc atcggtcggt
     1261 aaacaggacg gcgttgtctt tgccgatcag caggaaggac acgaaaacag ggttgaaagg
     1321 tacgtcgctg ccgcgcaggt tggtcagcca ggcgatgtcg tcaagcgagg aaaccaagtg
     1381 gtaatccgcg cctttttccg ccatcacggc gcgcacgcgg gcgagttttt cggcggcggt
     1441 ttcggagacg taggcggggt cgtggatgaa cacggtttcg gcggggatag cgggacggct
     1501 tgtccacact tggtcaagca gatcgtttgg gtgttcgatg cggatgtttt tggcggtcag
     1561 ggattgcgcc aaagtgcgtt tgccggtgag cgagaccata tcggaaggga tgccgacggc
     1621 ggcgttttcg ggcaggtttg ccgcgagcca ttcgttatac ggcggcactt gcccgctttt
     1681 ttgcagttcg atgccgctgc ccgcaagctg tttggcggct tgttcccaat agcggctgtc
     1741 cacccatacg cccgcttcat cggcggtaac gacgaatgtg ccgaccgagc cggtaaagcc
     1801 cgacaattcg cggcgcgcct gccagtgtgc gggcaggtat tcggacaggt gggggtcggc
     1861 ggaagggatg acgagcgcgt ccaagccttg cgccttcatg gcttcgcgca atgcggacag
     1921 gtaattcgat acggtattca taataattcc tccaatcggg ttttgcggct tcagacggca
     1981 tggacggaaa tctgaaatgc cgtctgaaaa acataaccat cttatcaaat cgccagcctg
     2041 ctgcaaaacg gacgggctga tcggatggtg cgtgtttgcg cgaaaaaagc gatgtaaggt
     2101 tgtttgttgt aaaattttag gcataaaatg tcgtttcatg cctttttttg gaagtaatac
     2161 ggaaaatata gtcaagtttg agcatgaagt tctaatagtg tgtgttggca ggtatttgga
     2221 ttacgttaca atacgcgctt tcatcaaaat ttccctaccg ggcggtatcc tgtttttgaa
     2281 gacggtagga tttgggtttg tttgggggca atcccgccca aattggagca atagataggg
     2341 ctgtgtccga atatgcggct ctgtgtgttg aaacacggtt aaaaatacgg aagactttat
     2401 gtccgaacaa catatttcga cttggaaaag taaaatcaac gcactggggc cggggattat
     2461 gatggcttcg gcggcggtcg gcggttcgca cctgattgcc tcgacgcagg cgggtgcgct
     2521 ttacggctgg cagatcgcgc tcatcatcat cctgaccaac ctcttcaaat acccgttttt
     2581 ccgcttcagc gcgcattaca cgctggacac gggcaagagc ctgattgaag gttatgccga
     2641 gaaaagccgc gtttatttgt gggtattcct gattttgtgc atcctctccg ccacgattaa
     2701 cgcgggcgcg gtcgccattg taaccgccgc catcgtcaaa atggcgattc cctcgctgat
     2761 gtttgatgcc ggcacggttg ccgccttgat tatggcatcc tgcctgatta ttttggtgag
     2821 cggacgttac cgcgctttgg atcgcgtttc caaaatcatc atcgttactt tgagtatcgc
     2881 cacgcttgcc gccgccggca tcgctatgtc gcgcggtatg cagatgcagc ccgattttat
     2941 cgagccgaca ccgtggacgc ttgccggttt gggcttcctg atcgcgctga tgggctggat
     3001 gcccgcgccg attgaaattt ccgccatcaa ttctttgtgg gtaaccgaaa aacaacgcat
     3061 caatccttcc gaataccgcg acgggatttt tgatttcaac gtcggttata tcgccagtgc
     3121 ggttttggct ttggttttcc ttgcactggg tgcgtttgtg caatacggca atggcgaagc
     3181 agtgcagatg gcgggcggca aatatatcgg gcaattgatc aatatgtacg ccgttaccat
     3241 cggcggctgg tcgcgcccgc tggtggcgtt tatcgcgttt gcctgtatgt acggcacgac
     3301 gattaccgtt gtggacggct atgcccgtgc cattgccgaa cccgtgcgcc tgctgcgcgg
     3361 aaaagacaaa acgggcaacg ccgaattctt tgcctggaat atttgggtgg cgggcagcgg
     3421 tttggcggtg attttttggt ttgacggcgt aatggcgaat ctgctcaaat ttgcgatgat
     3481 tgccgctttt gtgtccgccc ctgtgtttgc ctggctgaat taccgtttgg tcaaaggtga
     3541 tgaaaaacac aaactcacat caggtatgaa tgcccttgca ttggcaggct tgatttatct
     3601 gaccggtttt accgttttgt tcttattgaa tttggcggga atgttcaaat gataacgatg
     3661 ccgtctgaaa ccgcaaaccg ctttcagacg gcattgtcgc gtttatggaa aaaaatgccg
     3721 acatcgcgta aaatatgcgc aaattttgtg attcggtcag gtcgtctgaa acagattgcg
     3781 cctgatttat tttttcggaa aaccctatga gcgaacaaaa ccatccgcaa accgagccgc
     3841 agttggacga aaaccaaatc atcgccctgc gccgcgaaaa actgcacaac atccgccaac
     3901 agcgcaacgc ctatcccaac gacttcaaac gcgacagctt cgccgccgat ttgcacgccc
     3961 aatacggcga aatcggcaaa gaagaactcg atccgcaagg cattcccgtc aaagtggccg
     4021 gccgcatgat gctgaagcgt caaatgggca aggcgagctt tgccaccatt caagacgtgt
     4081 ccgggcaaat ccagctttat ctgaacaaca aaggcgtgag ccaagaagtt ttggacgact
     4141 tcaaccattg ggatttgggc gacatcgtcg gcgcggaagg cactttgttc aaaaccaacc
     4201 acggcgaact gaccgtacgc gtgtccgaca tccgcctgtt gtccaaatcc ttgcgtccgc
     4261 tgccggacaa acacaaaggc ttgagcgatc aggaaaccaa ataccgccaa cgctatgtcg
     4321 atttgattgc caacgaagaa tcgcgcaata cctttatcaa acgcagccaa atcatccaat
     4381 ccgtgcgtaa ttttatggtg ggcgagcatt atctcgaagt cgaaaccccg atgatgcacc
     4441 cgattcccgg cggcgcgacg gcaaaaccct tcgttactca tcacaatgcc ttagatattc
     4501 cgctttacct gcgtatcgcg cctgagctgt atttgaaacg cctggttgtc ggcggcttgg
     4561 aacgcgtgtt tgaaatcaac cgcagcttcc gcaacgaagg catgtccgtg cgccacaacc
     4621 ccgaatttac gatgattgaa ttttacgaag ccttctccga ctacgaacgt atgatgcaga
     4681 tggcggaaga catcatccgc aacgcatcgc gcacagtaaa cggtacggca aacatcactt
     4741 acaatggcaa agaagtcgat ttggaaagcc cgttcgagcg tctgaccatc ctcgaagcca
     4801 tcaaaaaata caatccgcac tacaccgacg agcagttgaa cgatgcggaa tggctgaaaa
     4861 aagaaatcgt caaacacggt gaaagcctgc cgccgtcccc gggcatcggc agcctgcaac
     4921 tcgcgctgtt tgaaggttgc gccgagggca agctgtggaa cccgactttc atcatcgatt
     4981 acccggtcga agtttccccg ttggcgcgcg cttcggatac caaacaaggt ctgaccgaac
     5041 gtttcgaatt gttcgttgtc ggccgcgaac tggcaaacgg ctattccgag ttgaacgacc
     5101 ccgaagacca agccgaacgc ttcaaagcgc aagtggcgca aaaagacgcg ggcgacgacg
     5161 aagcgatgca ctacgatgcc gactacatcc gcgcgatgga gttcggtctg ccgccgactg
     5221 gcggttgcgg tatcggcatc gaccgcttgg taatgttgct gaccgattcg caaaccatcc
     5281 gtgacgtgat tctgttcccg cagatgcgcc ccgagtaatc ataaaaacag ttgaaatggc
     5341 aatgccgtct gaacccgatt ggattcagac ggcattttgt atggcaggat tgatagtgaa
     5401 ttaacaaaaa tcaggacaag gcggcgagcc gcagacggta caaatagtac ggaacc
//
LOCUS       DAWFAD010000124         5424 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWFAD010000124 DAWFAD010000000
VERSION     DAWFAD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.124"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1..368)
                     /locus_tag="WGO49_001644"
                     /pseudo
     CDS             complement(1..368)
                     /locus_tag="WGO49_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            complement(453..668)
                     /locus_tag="WGO49_001645"
     CDS             complement(453..668)
                     /locus_tag="WGO49_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306730.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRP"
     gene            complement(956..1309)
                     /locus_tag="WGO49_001646"
     CDS             complement(956..1309)
                     /locus_tag="WGO49_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306731.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            1545..2972
                     /locus_tag="WGO49_001647"
     CDS             1545..2972
                     /locus_tag="WGO49_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ0306732.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSVGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3041..4444
                     /locus_tag="WGO49_001648"
     CDS             3041..4444
                     /locus_tag="WGO49_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ0306733.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(4608..>5424)
                     /locus_tag="WGO49_001649"
     CDS             complement(join(4608..5140,5140..>5424))
                     /locus_tag="WGO49_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ0306734.1"
                     /translation="PRYYEQCKNISQTAATFNLSRNTLYLWIRLKKQTGSLKHQVTGL
                     NAVKSDRQKPAQYIGQHQDAYLHEIAKHFDCTAATICYALKQMGITRKKRPTTYKEQD
                     PAKVTHYLTQPAEFSDYQRVYLDETGFDRYLFRPYARSLKGQIVKAQISGKRYQRLSL
                     VSAQVGNRLIAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREM
                     AEKLGHKVLPLAPYSPELNPIEKVWANIKQYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1368 a         1311 c         1396 g         1349 t
ORIGIN      
        1 gaaaccgaaa acttcttcgg cgagcatgga cgcggcgcga ataaaatctt cgtcttccaa
       61 aagacttaca cgaacgccgt atttatcgaa aaaggttttt tcatgaaatg aaaagctaat
      121 ttgatcaatg aacgccgaat cagatacacc gcgccgaaga ggaacgccta acaggtttcc
      181 tttaccgtcc gttatgtacg tttcgtaaca ttcgaagact tcctgaaccc tgcctgccgt
      241 ttcggtttct gtccccccct gttagataag gggggaagat ttgaagcggt tgccggcttc
      301 ctgccgtccg ctggcgcgtc cgtcatcacg ccggcaaccg cctttgtcat cccttgctta
      361 tcttccatgg tgcgaatcct caaaaacggg caaaaaaaag ccctgttact tgtagaaagt
      421 aaaggacgtt aatttttgtt aatcgtccct tcttagggac gcaatatata aggttttaaa
      481 acgctaagaa caaaatccga taacggctct gaagaagtaa gtctttcttt attgcacaag
      541 atagcaaact tccacggcat tctcgcccca cccgtgccgt ccaagccttt ttcaaagaca
      601 tagatggttt tcttggcaat cacttcgtca gtttggcggt cgataatccg tatggtcgca
      661 cctgccaccc agtgcctgcg cagcttggaa tcgacattgt tttcgaacgt taccgcataa
      721 cgggcggggt gtatatgatt aaatatttga tttatcggaa aatctttacc tgaataccga
      781 ataatatcgg aatggttggg ttgcaggaca tccacatagc ggtattcccc atcgctaaaa
      841 aatcctagga aacgagcaat aaaatttacg ccttcgctgg tctgtaaagc cgcattgtcc
      901 cacatcgggt ctctggtttt tgcatctgcc gaaacggtac gctcaggtac taccttcaac
      961 agcataatcc cttccacatt gtccgccgtc tggtaaatct ttcccccgcc gttttgcatt
     1021 gttcgttaaa cacggcttcg gcttctttgt attttctgtc ccactcttct tgtgcctgta
     1081 tttcttcttt gatcgggccg aattgtttgg gaataatcca aacaaacagc atcaggatag
     1141 cggcggcggt caggctgcct gaaaggattt tgccggggtt ccgtttgggc tttttatagg
     1201 caaagcggac gagaaaccaa agcaacagca gcatggtgcc ccaatagccg attgagaata
     1261 ggatggccaa accttctagg aaatggcgta aatcgtttgt ggtaaacatg ggttgttcct
     1321 gtggttaaat gtgcaggctg ctttttgccg aaccttgccg catctcaaaa gcagcctgcg
     1381 cttcagcgtt gcgttacgca gtaaaataat gaatatttgt aacgacttgg gtattttttg
     1441 tcaatattcc cgcctttccc ttaacagctg ccgcgctttc cgttaaaatt cctttacata
     1501 tttatattgt ttcctgtttc tatattgcca aggttatacc cgttatgttt ttctccgccc
     1561 tgaaatcctt tctttctcga tacattactg tatggcgcaa tgtttgggcg gtgcgcgacc
     1621 agttggaacc gcccaaacgc acggcggaag aacaggcgtt tttgcccgcg catttggaac
     1681 tgaccgatac gccggtctct gccgctccga aatgggcggc gcgttttatt atggcgtttg
     1741 cgcttttggc tttgttgtgg tcctggttcg gcaaaatcga tattgtggcg gcggcttcgg
     1801 gcaaaacggt gtcggtcggg cgcagcaaaa ccatccagcc gctggaaacg gcggtggtta
     1861 aggcggtaca tgtgcgcgac gggcagcatg tgaaacaggg agaaacgctg gcggaactgg
     1921 aggctgtggg aacagacagc gatgtggtgc agtcggagca ggctttgcag gctgcccaat
     1981 tgtccaaact gcgttatgaa gcggtattgg cggcattgga aagccgtacc gtgccgcata
     2041 tcgatatggc gcaagcacgg tctttaggtc tctccgatgc cgatgtgcaa tcggcgcagg
     2101 tgttggcgca gcaccagtat caggcatggg cggcgcagga tgcgcaattg cagtcggctt
     2161 tgcgcggcca tcaggcggaa ttgcagtcgg ccaaggcgca ggagcagaag ctggtttcgg
     2221 tgggggcgat cgagcagcag aaaacagcag actaccgccg tttgcgggcc gacaatttta
     2281 tttcggaaca tgcgtttttg gagcagcaga gcaaatcggt cagcaattgg aacgatttgg
     2341 aaagtacgcg cggtcagatg aggcagattc aggcggccat tgcacaggcg gagcagagtc
     2401 gggtgctgaa tacgcagaac ctgaaacgcg atacgctgga tgcgctgcgc caggcaaacg
     2461 aacagattga ccaataccgc ggccaaacgg ataaggcaaa gcagcggcag cagctgatga
     2521 caatacagtc gcctgcggac ggcacggtgc aggaattggc cacctatacg gtgggcggtg
     2581 tggtgcaggc tgcccaaaaa atgatggtgg ttgcgcccga tgacgacaaa atggacgtgg
     2641 aagttttggt attgaacaaa gacatcggtt ttgtggaaca gggacaggat gcggtggtga
     2701 agattgagag ttttccctat acgcgctacg gttatctgac gggcaaggtg aaaagtgtca
     2761 gccatgatgc ggtaagccac gaacagttgg gcttggttta tacggcggtg gtgtcgctgg
     2821 acaaacatac cttgaatatt gacggcaaag cagtgaatct gacggcgggc atgaatgtca
     2881 cggcggagat taaaacgggt aaacggcggg tgctggatta tctgttaagc ccgctgcaaa
     2941 ccaaattgga cgaaagcttt agggagcgat aggcggatcc gtactgggca tttgttatcc
     3001 gccggttcgg acatgcagac tgcctgaaac cattgcccgg atgacattgc tcaatctaat
     3061 gataatgcaa gattacggta tttccgtttg cctgacactg acgccctatt tgcaacatga
     3121 actattttcg gctatgaaat cctatttttc caaatatatc ctacccgttt cactttttac
     3181 cttgccacta tccctttccc catccgtttc ggcttttacg ctgcctgaag catggcgggc
     3241 ggcgcagcaa cattcggctg attttcaagc gtcccattac cagcgtgatg cagtgcgcgc
     3301 acggcaacaa caagccaagg ccgcattcct tccccatgta tccgccaatg ccagctacca
     3361 gcgccagccg ccatcgattt cttccacccg cgaaacacag ggatggagcg tgcaggtggg
     3421 acaaacctta tttgacgctg ccaaatttgc acaataccgc caaagcaggt tcgatacgca
     3481 ggctgcagaa cagcgtttcg atgcggcacg cgaagaattg ctgttgaaag ttgccgaaag
     3541 ttatttcaac gttttactca gccgagacac cgttgccgcc catgcggcgg aaaaagaggc
     3601 ttatgcccag caggtaaggc aggcgcaggc tttattcaat aaaggtgctg ccaccgcgct
     3661 ggatattcac gaagccaaag ccggttacga caatgccctg gcccaagaaa tcgccgtatt
     3721 ggctgagaaa caaacctatg aaaaccagtt gaacgactac accgacctgg atagcaaaca
     3781 aatcgaggcc atagataccg ccaacctgtt ggcacgctat ctgcccaagc tggaacgtta
     3841 cagtctggat gaatggcagc gcattgcctt atccaacaat catgaatacc ggatgcagca
     3901 gcttgccctg caaagcagcg gacaggcgct tcgggcagca cagaacagcc gctatcccac
     3961 cgtttctgcc catgtcggct atcagaataa cctctacact tcatctgcgc agaataatga
     4021 ctaccactat cggggcaaag ggatgagcgt cggcgtacag ttgaatttgc cgctttatac
     4081 cggcggagaa ttgtcgggca aaatccatga agccgaagcg caatacgggg ccgccgaagc
     4141 acagctgacc gcaaccgagc ggcacatcaa actcgccgta cgccaggctt ataccgaaag
     4201 cggtgcggcg cgttaccaaa tcatggcgca agaacgggtt ttggaaagca gccgtttgaa
     4261 actgaaatcg accgaaaccg gccaacaata cggcatccgc aaccggctgg aagtaatacg
     4321 ggcgcggcag gaagtcgccc aagcagaaca gaaactggct caagcacggt ataaattcat
     4381 gctggcttat ttgcgcttgg tgaaagagag cgggttaggg ttggaaacgg tatttgcgga
     4441 ataaagcagg ctgaaacggt tatgaaattc ccaaagcagc ctgcaccccg tttcgaaagt
     4501 gcaggctgct ttgggattga tccgatactt tcacattctc attgccttta aataaagcac
     4561 agttaaaaac acatttaaat taagtgcgaa ttaattcaaa tgtatattca attaaaatca
     4621 aaataggaca gtagtgcatc gtcaaatcgg gcgtaatcag acaaaacggt tcgcagatac
     4681 tgcttaatat tcgcccacac tttctcaatc gggttgagct caggtgaata aggtgcaaga
     4741 ggcaatacct tatgtcccaa tttttccgcc atttcccgta agacacccat acggtgaaat
     4801 cgcgcattat ctaaaataat caccgatttt tgagtcaatg cgggcagtag gcattgctga
     4861 aaccacgctt caaaaaagac tccggtcatc gtattttgat aaaccatcgg agcaatcagc
     4921 cggttgccga cttgtgcgga caccaaagat aagcgttggt atctttttcc acttatctgc
     4981 gctttcacta tttgcccttt caggctgcgg gcatagggac ggaacaggta gcggtcaaat
     5041 cctgtttcat ccaaataaac acgttggtag tcggaaaatt cggccggctg tgtcaaataa
     5101 tgcgttactt tggccgggtc ttgttctttg taagtggtgg tctttttttg cgcgttatcc
     5161 ccatctgttt gagtgcatag caaatggtgg ctgccgtaca atcaaaatgt ttggcgattt
     5221 catgcagata ggcatcctgg tgttgcccaa tatattgagc cggtttttgc ctatccgatt
     5281 tgacggcatt tagaccggta acttgatgtt ttaggctgcc tgtttgtttt ttaaggcgaa
     5341 tccacaggta aagcgtgttt cttgacaagt taaacgttgc tgcggtttgg ctgatgtttt
     5401 tgcattgttc gtaatatcgt ggat
//
LOCUS       DAWFAD010000125         5363 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWFAD010000125 DAWFAD010000000
VERSION     DAWFAD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.125"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(99..2930)
                     /gene="lbpA"
                     /locus_tag="WGO49_001650"
                     /gene_synonym="tbpA"
     CDS             complement(99..2930)
                     /gene="lbpA"
                     /locus_tag="WGO49_001650"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ0306735.1"
                     /translation="MNKKHGFQLTLTALAVAAAFPSYAANPETAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETHPHGDIADGVAYGINRLDAFRQTYGIKKPSEGGEYFLAEGESEL
                     KPVAKVAGNGNYLNNQLNRWVKERIEQNQPLSAEEEAMVREAQARHENLSAQAYTGGG
                     RILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAKKF
                     RDKSGVYDGDDFRDGLYFVPNIEEWKGDQKLIRGIGLKYSRTKFIDEHHRRRRMGLLY
                     RYENEKYSDNWADKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSS
                     DRFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISEYS
                     DYTDKGDKYLLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPNQAGQLTEIDIRDYYNAQNMSLQGVNILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(2927..5110)
                     /locus_tag="WGO49_001651"
     CDS             complement(2927..5110)
                     /locus_tag="WGO49_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0306736.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTPPPAKPSIEIMPVNRPAVGAAMRLPRRNTAFHREDGTEIPNSKQAEEKLSFQEG
                     DVLFLYGSKGNKLQQLKSEIHKRDSDVEIRTSEKENKKYDYKFVDAGYVYVKGKDEIK
                     WTSDYKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDAKRHRAGKAVGI
                     DNLGYYTFYGNDVGATSYAAKDVDEREKHPAKYTVDFGNKTLTGELIKNQYVKPSEKQ
                     KPLTIYNITADLNGNRFTGSAKVNPDLAKSHANKEHLFFHADADQRLEGGFFGDKGEE
                     LAGRFISNDNSVFGVFAGKQNSPVPSGKHTKILDSLKISVDEASGENPRPFAISPMPD
                     FGHPDKLLVEGHEIPLVSQEKTIELADGRKMTVSACCDFLTYVKLGRIKTERPAAKPK
                     AQDEEDSDIDNGEESEDEIGDEEEGTEDAAAGDEGSEEDEATENEDGEEDEAEEPEEE
                     SSAEGNGSSNAILPVPEASKGRDIDLFLKGIRTAETNIPQTGEARYTGTWEARIGKPI
                     QWDNHADKEAAKAVFTVDFGKKSISGTLTEKNGVEPAFRIENGVIEGNGFHATARTRD
                     DGIDLSGQGSTKPQIFKANDLRVEGGFYGPKAEELGGIIFNNDGKSLGITEGTENKVE
                     ADVDVDVDVDVDVDVDADVEQLKPEVKPQFGVVFGAKKDNKEVEK"
BASE COUNT         1171 a         1444 c         1227 g         1521 t
ORIGIN      
        1 atcttgttta tatcgaatca aaaaaacctg caccttaatc agttggcggt ttagtccgac
       61 ttttggggtg cagatcaagc tttcagacgg tatttccttt aaaacttcat ttcgagcgcg
      121 agactgaagt tcctgcccgg tgcggcatac cttccatagt tgctgtcgcc gccgtgccgg
      181 tttgccgtgc tttccgcggt ctggcgcaag gattcccaag taacgtagcg gtagttgccg
      241 atattgtaga tagccgccct caaggtcagc cgttttttca gattcagata ggcggaaacg
      301 tctgccgtcg accaagaaga cgacgctctt tttgtcgaat atcgtttttg atcgcctgcc
      361 agataagcaa gctcgtcagg gtttttccct ttggaatagg tcagcataat gtttgcgccc
      421 catttcccct cgggctggtc gtatccgaac cccaaaacat aacgcgacgg ctgtaccgca
      481 tccaaagcat agctgcggag agacagtccc ggccggttgg ataccgattt cggtttgata
      541 cggttgtacg ccaaagtcgt gtacaaacct tcgggcagtt tgccgtacac accgttccag
      601 tcgatttttc ccaatatatt aacgccttgg agcgacatat tttgggcatt gtaataatcg
      661 cgtatatcaa tctctgtcaa ttgtcctgcc tgattcggca atttggtttt gtgatcggca
      721 acggcaatca tatcggtata acggttgcgg aagctgctga tttccaaaaa gccgaaatcg
      781 cccttccact gcaaaccgat ttcgcggttg gctgcctttt ccgatttcag ggcgggacgc
      841 tgccagcctt tcggataatc gtgataaatg tctatcccga aaagttcttg gaatgagggc
      901 gttctgaagc cgctggaggc gcggtaagac aaagaaaaat gccggttcgg tttgaacacg
      961 atgccgctgt tccacgaacg gtcaacatac cgcccgctgc ggacgagttc ttccgacgtg
     1021 gtgaagtttt tccggtcgta cctgccgccc aagctgaaat cgaaatattt gccgattgaa
     1081 aaacggtcgt tcaaagaaat gtggatattg ctgccgttga tttttcttgg cacgcatttg
     1141 cgggaacgca gggtttcgat gtagccgcag accgaccctt cgacgacttc gggcttaccc
     1201 aaaagatact tgtctccctt atcggtgtaa tccgagtatt ccgaaatcct tgccgcattg
     1261 tgagaaagct gttcggggcg ggaaatcgcg ttggaagcat cgtaaccgaa gcccaaagtc
     1321 agatggtgtt tcgtccattt gtttttcagc gatttctcaa acgaggcatt caaaacattg
     1381 tgctgttcgc ggtagtggaa acggtcgctg ctgtcgtagg aatacggttt gtccgccgac
     1441 gcgcggcagg atttgtccac ggcaggatac acggcgcaat tcagcttcag cgtgttgttg
     1501 tcggttgcca cgccctgttt gtcaaacgac aacaccgcct tatccgccca gttatccgag
     1561 tatttctcat tttcataacg gtacagcaaa cccatacggc ggcggcggtg atgttcgtca
     1621 ataaatttgg tgcgggaata tttcaaacct atgcccctaa tcaacttttg atcgcccttc
     1681 cactcttcta tattcggcac aaaatacaag ccgtcgcgga aatcgtcgcc gtcgtacacc
     1741 ccgctcttgt ctctaaactt tttcgcctcg tccgtaccgt aatactgttt ttccgtcata
     1801 tcgcggatat cgtaacgctg tttggtatcc tcaaacacgc cgccgacata atgcctgccg
     1861 ccgaagcggt agcccagctt ggcaagccac gaaccgctgc ggtaatccat cggatcgggc
     1921 aatatcctgc cgccgcccgt gtaagcttgg gcggacaggt tttcgtggcg cgcctgcgcc
     1981 tcccgcacca tcgcttcttc ttcagcactt aaaggctgat tttgttcaat acgttctttt
     2041 acccagcggt tgagctggtt gttcaaatag tttccgttgc ctgccacttt tgccacaggc
     2101 ttgagctcac tctcgccctc cgccaggaaa tattcccctc cttcacttgg ttttttaatg
     2161 ccgtatgtct ggcggaacgc gtccaaacgg tttatgccgt atgccacccc atccgcaata
     2221 tcgccgtgcg ggtgcgtttc ccgcccctga cgttcggttc ggattagcag cccttcccaa
     2281 ccgtctttac tgaaacccgc gccgagcgac ttcataaatt ggcggttttt actgccgtag
     2341 gcggtttttg cctgtatccc ccagcttttg ccgtctgaaa tcaggtctgc cgcctctttg
     2401 gtgcggaaag cgaccgcgcc gccgagtgcg ccgctgccgt gatcggacga accggcacct
     2461 ttgtcgattt ccaccgtgct gatgttttca tattcgattt cgttgattgc accgctgccg
     2521 ccgcgtccgc cgtatccgct caacgatccc tgcacggtaa acgcctgtat ttgggcaacg
     2581 ccgtcaaccg aaaccgccac acggttttta tccacgccgc gtatcgagta gccgccgctc
     2641 gcgccgttgc cctgttcgac aaccgccacg cccggatcgt agcgcgtcag gtcgcggata
     2701 ccgagtacct gttctttgtt caacgtttcc gacgttttga cgattttgcc caaaccggtc
     2761 gcctctttcg atcgccgtcc cactttggca gcacggacgg taacctcttt cagggattgg
     2821 gtctgcgccg catcaggtgc cgccgtttca gggtttgccg catatgacgg gaaggcggcg
     2881 gcaacagcca aggcagtcag ggtaagctga aaaccgtgtt tcttattcat ttttccacct
     2941 ctttattatc tttcttcgca ccgaatacca cgccgaattg gggtttaact tcaggtttta
     3001 actgttcaac atcagcatca acatcaacat caacatcaac atcaacatca acatcaacat
     3061 cagcttcaac tttattttca gtaccttcag ttataccaag agatttccca tcattattga
     3121 aaataatacc gcccaattcc tccgccttcg ggccgtaaaa tcctccttct acacgaagat
     3181 cattagcttt gaagatctgc ggtttggtcg aaccctgccc ggaaaggtcg atgccgtcat
     3241 cccgagtgcg cgctgtcgca tggaaaccgt tgccctcaat cacgccgttt tcaatacgga
     3301 aagcaggttc tacaccgttt ttctccgtca gcgttccgga aatcgatttc ttgccgaaat
     3361 caacggtaaa tactgctttt gccgcttctt tatccgcatg attgtcccat tgaatgggtt
     3421 tgccgatacg cgcttcccaa gtgccggtat agcgtgcttc tccagtttgc ggaatattcg
     3481 tttctgccgt gcggatacct ttcaggaaaa ggtcgatatc cctgccttta gaggcttccg
     3541 ggacaggcag gatggcgttt gaactgccgt tgccttctgc cgacgattct tcttcaggtt
     3601 cttcagcttc gtcttcttcg ccgtcttcgt tttctgtggc ttcgtcttct tcgctgcctt
     3661 catctcctgc ggctgcatct tcggtgcctt cttcttcatc gccgatttcg tcttcgcttt
     3721 cttcgccatt atcaatgtcc gaatcctctt cgtcctgcgc cttcggtttg gcggcggggc
     3781 gttcggtttt tatccgtccg agtttcacat aggtcaaaaa gtcgcaacaa gcactgacgg
     3841 tcattttcct gccgtcggca agctcgatgg ttttctcttg gctaaccaaa ggaatttcat
     3901 gcccttcgac aagaagtttg tcgggatgac caaaatcggg cataggagaa atggcaaacg
     3961 gtcggggatt ttcaccactt gcctcatcaa cggaaatttt cagagaatcc aagattttgg
     4021 tgtgttttcc agacggcacg gggctatttt gtttgcctgc gaatacaccg aatacgctgt
     4081 tgtcgttgct gataaaccgt ccggcaagct cttccccctt atcgccgaaa aaaccgccct
     4141 caagccgctg atcggcatcg gcatggaaaa acaaatgctc cttattggca tggcttttcg
     4201 ctaaatcagg attgaccttg gcactgccgg taaagcggtt gccgtttaaa tcggcagtga
     4261 tgttgtaaat ggtcagcggt ttttgcttct cactgggttt gacatattgg tttttaatca
     4321 gctcgcccgt cagggttttg ttaccgaaat ctaccgtata tttagcagga tgtttttccc
     4381 tttcgtcgac atccttagcc gcataagaag ttgcaccaac atcgttacca taaaatgtgt
     4441 aataacccaa attgtcaatg ccaaccgcct tacctgctcg atgacgtttg gcatcggtca
     4501 tatattgcca gttaccagaa tattccaccg ttcccgcact cggtaaagat tgggaaggac
     4561 gttctccgga ataatataca aaaccgtcat aacctaagcg gttggaaaac tgcttgtaat
     4621 ctgaagtcca cttaatttca tcttttccct ttacatatac ataacctgca tctacaaatt
     4681 tataatcata ttttttattt tccttttctg atgtcctaat ttctacatcg gaatcacgtt
     4741 tatgaatttc gcttttaagt tgttgaagtt tatttccttt tgaaccgtat aaaaacagaa
     4801 catcaccttc ttgaaacgac agcttttctt ctgcttgttt gctatttgga atttccgtgc
     4861 catcttcacg atgaaaagca gtattccgcc ttggcagccg cattgccgca ccgacggcgg
     4921 gccggttgac cggcatgatt tctatagaag gtttggcagg gggcggagtg ggaacgtcct
     4981 tagacttgaa agtgacgggg tacgcggtcg gcgttgattc gacaacaggc tgcacgccga
     5041 aattgccgcc gatacaagat gccaaaagta agggcaacaa gacaatgccg ccataattcg
     5101 gtttacacat ccctactttt cctcctattt gattaataat aattatcatt atattaatat
     5161 gtacagataa tatcaacccg tttttatagt gaattaacaa aaatcaggac aaggcgacga
     5221 gccgcagaca gtacagatac attccgtcat tcccacgaac ctacattccg tcattcccac
     5281 gaacctgcac cacgtcattc ccacgaaagt gggaatccag ttcgttcggt ttcgcttgtt
     5341 ttaagtttcg ggtaacttct act
//
LOCUS       DAWFAD010000126         5321 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWFAD010000126 DAWFAD010000000
VERSION     DAWFAD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.126"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            295..1728
                     /locus_tag="WGO49_001652"
     CDS             295..1728
                     /locus_tag="WGO49_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ0306737.1"
                     /translation="MNEYSQLIKHPDISLSPISDSIGIGNPATGEILAYVRNTGSDKL
                     KNLIQKAAAAQKLWAAKTALERADILWRWYFLVKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALASLAYEAGIPQDLLPVVSGSASEIGHEFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSAIYDEFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGASCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            1837..2625
                     /locus_tag="WGO49_001653"
     CDS             1837..2625
                     /locus_tag="WGO49_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ0306738.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDQWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            2993..4549
                     /locus_tag="WGO49_001654"
     CDS             2993..4549
                     /locus_tag="WGO49_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0306739.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADNQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQCAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            4566..5312
                     /gene="surE"
                     /locus_tag="WGO49_001655"
     CDS             4566..5312
                     /gene="surE"
                     /locus_tag="WGO49_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ0306740.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKNPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
BASE COUNT         1248 a         1341 c         1515 g         1217 t
ORIGIN      
        1 gtttggattt ttgccatact ctaccacacg ttgcaactgc aatctttgtt ccttatatat
       61 tatcaaaggg ctgcattact tttctttcta aattaccatt gtataaaaag tatttttctt
      121 aaacgacagg gattgcgccc gcgccatatt cagacaccgc ccggatgttg cactgcccga
      181 tcggatgctt cagacggcat cggaagggtt tgcagttttg gaatatgaag atgataatgt
      241 ccgcgagatt gacggcattt gaaagcggtt attccgcata ccggaggata cgaaatgaac
      301 gaatattccc aattaatcaa gcatcccgat atttcccttt ccccgatttc agacagcatc
      361 gggatcggca atccggcgac gggtgaaatt ttggcgtatg tccgcaacac gggttcggac
      421 aaactgaaaa acctgattca aaaggcggcg gcagcgcaaa aattatgggc ggcgaaaact
      481 gctttggaac gcgccgacat attgtggcgt tggtattttt tagttaaaga aaacaaagaa
      541 gcactggcgc gcctgatgac gatggagcag ggcaaaagcc tgaccgaggc gcgcggcgag
      601 attgattatg cggcttcgtt tgtgcgctgg tttgcggaag aggcgcggcg gattgacggc
      661 gatgtgttaa cgagtgtaaa agcgtcgcaa aaactggttg tgttgaaaca gcctgtcggc
      721 gttaccgccg cgattacgcc gtggaacttc ccatctgcga tgattgcgcg caaggccgcg
      781 cctgctttgg cggtgggttg cgcgatgatc gtcaaacccg catcgctcac gcctttgagt
      841 gcgtatgcct tggcttcgtt ggcttacgaa gcgggcatac cgcaggattt gttgcctgtt
      901 gtcagcggca gtgcttcgga aatcggccat gaatttgcta cgaacccgat tatccgcaaa
      961 atcagcttca ccggctcgac cgaagtcggc gcaaaaattt ttgccgacag cgcggcggac
     1021 attaaaaaac tcagtttgga gctgggcggc aacgcgccgt ttatcgtgtt tgacgatgcc
     1081 gatttggaca aagccgtcga aggcgcgctc gccagcaagt tccgtaacag cggtcagacc
     1141 tgcgtctgca ccaaccgcgt ttacgctcaa tccgccattt acgacgaatt ttgccgcaaa
     1201 ttgagtgaaa aagcagccgc gctcaaattg ggcaacggct tggaggatgg tgtgaaccaa
     1261 gggccgctga ttgaggaaaa agcggtggag aaagtcgagc agcacatcgc cgacgcgctt
     1321 gctaaaggtg caagctgcct gaccggcggc aaacgcagcg cgttgggcgg aacgtttttc
     1381 gaaccgactg ttttaagcgg cgtaacggcg caaatggcgg tggcacgcga agaaaccttc
     1441 gggccgttgt gtccggtatt ccgttttgaa accgaagccg aggtcatcga ggctgcgaac
     1501 aatacggaat acggtttggc agcttacctt ttcaccgccg acaccgcccg ccaatggcgc
     1561 gtcggcgaag ccttggaata cggtatggtc ggcatcaata cgggcttaat cagcaatgaa
     1621 gcggcaccgt tcggcggcgt gaaacgtagc ggtttgggac gtgaaggcag caaatacggt
     1681 gcggacgaat atctagaatt gaaatatctg tgtatggatg tcgggtgacg gatgccgtct
     1741 gaacggcagg atttcagacg gcgccgcatt ttaagcagtc tctatctgtt gtacaatgcg
     1801 ccctgttttt acggttattt ttgatttgaa caagatatga tggaaaacgg aaaaacagtc
     1861 ccgagatggc gttttgcctt gaaaagtgcg ggctggcacc tcttaatcag cctgtcggtt
     1921 gcagggctgg cggcattgct ggtttttaag gtttggtatc cttatcctta tgccgagctg
     1981 acgggagggc tgtcgcttta tcagctggtg gtggctgtcg atattgtatg tggtccgctg
     2041 ctgactttaa ttttggcaag cccgaagaaa aagacaaagg cacgcatggt cgatttttcc
     2101 atggtcggca tcatccagct ggcggctttg gtgtacggtc tgcacagcgt ttcgctggcg
     2161 cgtcctgtgg tggaagcgtt tgaacaggat cgtatgacca ttgttacggc ggcggaagtc
     2221 gtggtcgaag atttgcacaa agcccccgaa gggctgcaaa gcctgtcgtg gttcggcatc
     2281 cgccgcattg cattgaaaga acctgaggat gcggatgaga agaacaagac gctggatttg
     2341 tccctgaaag gtatcgagcc gagtatgcgt cccgaccagt ggctgccgta ttccgacaag
     2401 gaagcagaag aaatccgcaa acatctgaaa ccgctgaaag tcttggcgga tgcgagaaaa
     2461 acgacggttg cggacattct gaaacaggca ggtctcgccg aaggggagga gctgtattac
     2521 ctgccgttta ccagcagcag gcagaaagag tggatagtca ttaccgataa agagggcaac
     2581 accaaaggct acgcgccgat agacggcttc atcatcaccc cttaagcgtt gggactccgt
     2641 ccgcactcga acatccgttc ttcgcggcgg tagaatcaga ctgtatttga gaggggcgca
     2701 acccctcaat cagggcaaca cataggggaa ggctttatgt gccgtccgtg tgttgaaaca
     2761 tttcaaaata agctcatgcc gtccgaacat cctttcagac ggtatggcat tttgccattg
     2821 ccccgataag ctgttaaact atttaaatta tttcgaccga aggtacacgc acatgcaaga
     2881 ccccgagcag agcagcaagc ccgcccgccg ttttttgtgc gtaccgtctg ttcaggcagc
     2941 ttgagcgctt caggctgcct tttcgcgttt gaacttgagg aatacgaaaa tcatggattt
     3001 cagttggttg gcagaaccgc atacctggat aggttttgcc acgcttttgg tgttggaagt
     3061 tgtattgggg atagacaatc ttgtctttgt ggcgattttg gcaaacaagg tccagcccgc
     3121 acggcgcgac cgcgcacgga ttatcgggct ggggctggca gtcgtcatcc gcatcattat
     3181 gcttgctttt atggcgcaca tcatcacgct gaccgagccg ctgttccaaa tcggtggcct
     3241 cgccgtttcc ggcaaggaca tgattatgct cgcgggcggt attttcctgc tttacaaagc
     3301 caccaccgaa ctgcatgaac gcctcgaagg gcacaaccgt tttaccgttg ccgacaacca
     3361 aaaaaaacac gcgccgtttt ggggcgtggt cgcgcaaatc ctgatactgg atgccgtgtt
     3421 ttccatcgat tcggtcatta ctgcggtggc gatggtcgat catatcgtcg tggcgatggg
     3481 tgcggtcgtc gtcgcgatgg ctgtaatgat ttctgccagc aaactcttga ccgaatttgt
     3541 cgacagacac cctaccgtcg tgatgctctg ccttggtttt ttgttgatga tcggtttcag
     3601 cctgattgcc gaagccttcc atttccacat tcccaaaggc tacctctacg ccgccatcgg
     3661 cttctcgatt ttaatcgaat tgtttaacca gatttcgcag cgcaacagcc gcaaaaacga
     3721 ctacatcggc agctcgtggc gcaagcgcac cgccgaaaac gtcttgggta tgatgggtat
     3781 acgcgaaagc gtgcttgccg acgcgggcgg cgaatccggg gacgacgcgc attttgaaga
     3841 aaacgaaaaa tcgatgatac gcagcgtgct gacgcttgcc gaacgcccga ttatgggggt
     3901 gatgatccca cgccgcgaca tcgaacggct ggacatttcc caaagccgcg aagaacagtg
     3961 tgcccaactg caaaacacgc cttacagccg cctgctcgtt gtcggtaagg cgggcgtgga
     4021 cgaacctttg ggctacatca acaaaaaaga cctgctgtcc caactgctgg aaacaggcgg
     4081 tctcgacatt cagacggcat tgcgccagcc actcgtcctg cccgacagca ccaccgcgct
     4141 gggcgcaatc gaactcttcc gccaaagcag cgcggattat gctttggtgg tggacgagtt
     4201 cggcgcggta ttgggcatgg taaccatgaa agacctgctc gaaaccatcg caggcgagtt
     4261 ccccgaagaa tttgagcgcg aagaagaacc agccgttcag gggaatcccg atgaaagcct
     4321 gacggtggaa ggcgcgttgg aatatgtgga actcgcaccg caactcaacc tgccgcagca
     4381 ggaggaagat gccgatttcc atacggttgc cgggctgatt atggaagaat tgcaaaccat
     4441 ccccgatgtc ggcgattttg ccgatttcca cggctggcgg tttgaagtgg tcgaaaaaga
     4501 agggcagcgc atcgagcggg tcaaaatcac caaattgccc gaagaataag cattcaggat
     4561 agaaaatgaa cgttttgatt tccaacgacg acggctacct ctccgaaggt attgccgttt
     4621 tggcgcgcgt tacggcggaa tttgccaacg tcagggtggt tgcgcccgaa cgcgacagga
     4681 gcggggtcag caattcgctg acgctggaac gccctttgca gttgaaacag gcgcaaaacg
     4741 ggttctacta tgtcaacggc acgccgaccg actgtatcca catcgggcag tctgtatttt
     4801 cggattttca ggccgatttt gtcttttcgg gcatcaaccg gggcgcgaat atgggggacg
     4861 acacgcttta ttcggggacg gttgcggcgg caaccgaagc ctaccttatg ggcatacccg
     4921 ctgtggcgtt ttccttaaac gacgcttccg gacgctattg ggcaaccgca gaaaaggcac
     4981 tgtggacatt gttggcgcat tttttcaaaa accccccgca gtcccctatt ttgtggaaca
     5041 tcaatatccc cgccgtcgag ccggaagatg tgcggggcat taaaatcgcc cgtttgggca
     5101 ggaggcatca cggtcagaac gtcatccccg cgcgcaatcc gcgcggcgaa cagatttatt
     5161 ggataggccc ggtaggcagg gtttccgaca aagaagaggg aacggatttc ggtgaatgcg
     5221 gcgcaggttt cattaccgta acgccgctgc aaatcgacct gaccgcctat ccggacatgg
     5281 cggaaacagc ggcgttctgg catgcggact gaccgtttca t
//
LOCUS       DAWFAD010000127         5255 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWFAD010000127 DAWFAD010000000
VERSION     DAWFAD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.127"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            109..804
                     /gene="pgl"
                     /locus_tag="WGO49_001656"
     CDS             109..804
                     /gene="pgl"
                     /locus_tag="WGO49_001656"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ0306741.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            785..1771
                     /locus_tag="WGO49_001657"
     CDS             785..1771
                     /locus_tag="WGO49_001657"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ0306742.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1821..2669
                     /gene="hexR"
                     /locus_tag="WGO49_001658"
     CDS             1821..2669
                     /gene="hexR"
                     /locus_tag="WGO49_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ0306743.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2790..4436
                     /gene="pgi"
                     /locus_tag="WGO49_001659"
     CDS             2790..4436
                     /gene="pgi"
                     /locus_tag="WGO49_001659"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0306744.1"
                     /translation="MKHFHDLPAWPKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDESAVAKHFVAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPAILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLIRLYLQTNRK"
     gene            4450..5001
                     /locus_tag="WGO49_001660"
     CDS             4450..5001
                     /locus_tag="WGO49_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0306745.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHVCSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKFSELACKTAETEARLEELH
                     NRKKALIDEMAREADKKELPKRL"
BASE COUNT         1334 a         1465 c         1392 g         1064 t
ORIGIN      
        1 cagtcccgat atttggtatc aaaagcataa acaactgttc ggccgacata ttgctcaacc
       61 cgtttccata acggaatatg ccgtctgaaa taaaaaaagg acacaaatat gtttgtttgg
      121 cacgaatacg aaaatgcggc agaagcggcg cagtctttgg ctgacgcagt ggcggatgct
      181 ttgcagggcg cactggacga gaagggcggt gcggtgttgg cagtttccgg cggacgttcg
      241 ccgattgcat ttttcaacgc cctgtcgcaa aaagatttgg attggaaaaa cgtcggcatc
      301 accttggcag atgaacgcat cgtgccgacc gtccacgccg acagcaatac cggtttggtg
      361 cgcgaatacc tgttgaagaa caaagcggaa gcggcaatgt ggattcctat ggtggaagac
      421 ggaaaaactg aaaccgaatt acatcccgat gctgttgtcg attatgcact gaaacattac
      481 aaacagcccg atgttttggt tttgggtatg ggaaacgacg ggcatacggc ttcgattttc
      541 ccgaaagctc cgcagtttca gacggcaatc gacggttcgg caggtgtcgc gctggtgcat
      601 accacgcccg ttaccgcgcc gcacgagcgc gtcagtatga ccttggatgc gattgcccat
      661 acggggcatg tgtttttggc gatacggggc gaagagaaaa aagccgtgtt cgaccaagcc
      721 gcacaaggcg aaaaccgcga atatccgatc aacctcgttt tgaaccatca aggagtgaac
      781 tgccatgtct tctacgccga ataaacaagc cggatatccc cgactggtcg ccgatatcgg
      841 cgggacgaat gcacgctttg cgctggaaac cgcgccgcgc gtcattgaaa aagccgccgt
      901 gcttccgtgt aaagactacg atacggttac cgatgcggtg cgtgcctatc tgaatcaaag
      961 cggtgcaaca gccgtacggc acgcggcatt tgccatcgcc aacccgattt tgggcgactg
     1021 ggtgcagatg accaaccacc attgggcgtt ttccatcgaa accacccgtc agactttggg
     1081 gctggacacc ctcatccttt tgaacgactt taccgcgcag gcattggcgg taacgcagac
     1141 ttcaagcaaa gacctgatgc aggtaggcgg gcaaaagcct gtcgaatttg cccccaaagc
     1201 cgttatcggc cccggtaccg gcctgggcgt gagcggattg gtgcacagcc acgcaggctg
     1261 ggtggctttg gcgggcgagg gcgggcatac cagtttcccg ccgtttgacg atatggaagt
     1321 gctgatttgg cagtacgcca aaaacaaata cggccatgtt tccgccgaac gctttttgag
     1381 tggcgcgggc ttgagcttgg tttacgaggc tttggctgca aaacagaaag ccaaacccgc
     1441 caaactgatg ccgtctgaaa tcacggaaaa ggctttgagc ggcgcgtcgc ctttgtgccg
     1501 tcagactttg gacatcttct gcgccatgct cggcacggtt gcttccaacc tcgccctgac
     1561 gctgggcgcg cgcggcggcg tgtacctgtg cggcggcatt attccccgcg tgttggaata
     1621 tttcaaaact tccccgttcc gcagccgttt cgagaacaag ggcaggtttg aagcatatct
     1681 tgccgcgatt cccgtgtatg tcgtcttgag cgagttcccc ggaatttccg gtgcggctgc
     1741 ggctctcgac aaccatttga gaaacgttta accacagcgg ctccttgcag cggggctgca
     1801 ttatcgaagg gcatatcatt atgttaagca aaatcagcga atcactggca aacctttccg
     1861 gtgcggaacg caaagtcgcc gaatgtgcat tggcggaacc caaatggttc gttcatgccg
     1921 ccgttgccga aattgccgaa cgtgcgtccg tcagccagcc gaccgtcatc cgattctgcc
     1981 gcagcttggg ttataaaggg ctgcccgagt tcaagctcgc cttgtccgcc agcatcggtc
     2041 atgagggtat gccctatgtc cacgaagaac tcaatgccga cgacgatatg gcaagcgtgg
     2101 tcgagaaagt gttgggcaat gccgccgcct cgctgttggg cgaacgccgc ttcctgaaag
     2161 agtcggagct ggaaaacgcc attgccacgc tgatgcacgc ccgtcgcgtc gagttttacg
     2221 gtgtcggcaa ttccggtatc gttgcgcagg acgcgcaaca caagtttttc cgtttcggaa
     2281 tgtccacggt cgcctatgtc gatacgcaca cgcagctgat ggcagcatcc gttttgagcg
     2341 atcaggatgt tttggttgcc atttccaaca cgggttcgtc tatcgaactt ttggatgcgg
     2401 tcagcatcgc caaagaaaac ggcgcgtccg tcatcgcact gacccgcaac gattcgcctc
     2461 tggcgcaact tgccgactgc gtgttgagcg ttgccacaca ggaaaatgcc gaactctaca
     2521 cgcccatggt ttcccgcctc ttgcagcttg ccgtcatcga cattctcgcc atcggacttg
     2581 ccctgcgctt gggcgatgct gccagcctgc aattgcagaa aagcaaaaaa agcatacaca
     2641 acaagcacat cgattacgac aaagattgac cttcagacgg catcccacaa atgccgtctg
     2701 aaatgccgaa caacggccgt cggcggcttg cggcagtttc cggcagcctt ttcatcccac
     2761 aacaaaaaac ctcattcagg agcatataga tgaaacactt tcacgactta cccgcatggc
     2821 cgaaattgtg gaaccacttt gacgacagca aaacattgca tatgcgcgaa atgttcgagc
     2881 aagacccgca gcgtgcggaa cgctactggc tgcaagtcgg cggactgacg ctggactact
     2941 ccaaaaaccg catcaacgac gaaaccatgt cgcttttgtt cgagttggcg cgtgaagcag
     3001 gcgtgccgga gcggatgcgg cagatgttcc acggcgaaaa aatcaatacg acggaaaacc
     3061 gcgccgtcct ccacgtcgcc cttcgcaacc gcaccaattc gccgattgtg gttgacggtg
     3121 aagatgtgat gccaaaagtc aaccgcgttt tgcaacgtat gggcgaattt gcacacgaag
     3181 tccgcagtgg aagctggctg ggctatacca accaagtcat taccgacgtt gtcaacatcg
     3241 gcatcggcgg atcggatttg ggcccgttga tgatgtgtac cgcgctcaaa cctttcggtc
     3301 atccacgcct caatatgcac ttcgtctcca acgtggacgg ctcgcaactg cgcgacgtat
     3361 tgtccaaagt ccaccccgaa accacgctgt tcattatcgc ctccaaaacc tttaccacgc
     3421 aggaaacgct gaccaacgcg ctgaccgcgc gcgaatggtt tttgaatcat gcgggcgatg
     3481 aaagcgccgt cgccaaacat ttcgttgctg tttccaccaa ccaaaaagcc gttgccgaat
     3541 tcggcatcga caccgccaat atgtttgaat tttgggactg ggtcggcggc cggtacagcc
     3601 tgtggtccgc catcggattg ccgattatgc tgtatctcgg cgaagaaaac ttcatcgaaa
     3661 tgctcaacgg cgcgcacctg atggaccaac atttcatcaa cactccgctc gagcgcaacc
     3721 tgcccgccat cctcgccctt atcggcatct ggtacatcaa ctactacggc ggcggaagcc
     3781 acgtcatcgc accttacgac caacacctcc accgcctgcc caaattcatc cagcagctcg
     3841 atatggaaag caacggcaaa caggttacgt tggacggcaa agcagtcgga cacgaaacct
     3901 cgccgattat ttggggcgaa acgggcatca acggccagca cgccttcttc caactgctgc
     3961 accaaggcac gcacatcacc cccatcgacc tcatcgcctc gcttgaaaaa cgcagcaacc
     4021 tgcccggaca ccacgaaatc ctgcttgcca acgtcttcgc ccaagcagaa gcctttatgc
     4081 gcggcaaaac ccccgacgaa gtccgcgccg aactcaaagc gcagggtatg gatgaggcgc
     4141 gtatcgaaga gctggttccg cacaaaacct tctccggcaa ccgcccgacc aacctcatcc
     4201 tgatggataa ggtcaacccc cgcaatatgg gcagcctgat tgccatgtac gaacacaaaa
     4261 ccttcgtaca aggcatcatt tggggcatca acagcttcga ccagtggggc gtggaactcg
     4321 gcaaacaact ggctaaaacc attttgggcg aactgaccgg cgaaaccggg ccgcaaaagc
     4381 acgacagttc gaccgaacgc ctgatcaggc tttacctgca aaccaaccgc aaataaaacc
     4441 tgcggaaaaa tgccgtctga acgccgaccg tttcaaacgg catttttatc gaacaggaaa
     4501 accgtcggta aacttgcaga gcgtgtgcaa tcccgatatg atggtttgca taaatttaaa
     4561 catgtatgtt ccgcagctat ggcactgatt aaagagccgt tggacaaagc gaaacaaagg
     4621 aacgaagaac ttgaagcggc agaagaagcg gcggcgcagg aggcattggg tcgggagcag
     4681 gaagtcgacc gcgtatccga atgggaagaa cgctacaagc tgtcgcgcag cgagttcgag
     4741 caattctgga aaggattgcc tcaaaccgta cagaataagc tgcaagcctc acagaaaaca
     4801 tggaaaagcg ggatggataa aatctgtgcc aacaatgcga aagctgaagg tgaaacgcca
     4861 aacggcataa aattcagcga actggcatgc aaaacggcgg aaaccgaagc acgcttggaa
     4921 gagctgcaca accgtaaaaa agcccttatc gacgaaatgg ccagggaagc ggacaagaaa
     4981 gaactgccaa agcggctctg aacagcgcgg ttcaggcact gcccgccgat attgccgaaa
     5041 ccgtcatgcc cgagtaccgc aactgacaaa acggtttgaa tgccaaatgc gccgacggcg
     5101 acgaatacgg cgtggcacaa tctgactgcc gtaccagaga aatcaatgcg aaaaccaaag
     5161 aaatccaagg ttatctgatt gactgaaact tggatgcggg aatgtcggcg gcttttgcgt
     5221 ttttgtcgtt tttatagtga attaaattta aaccg
//
LOCUS       DAWFAD010000128         5198 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWFAD010000128 DAWFAD010000000
VERSION     DAWFAD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.128"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..480
                     /locus_tag="WGO49_001661"
     CDS             <1..480
                     /locus_tag="WGO49_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306746.1"
                     /translation="AAPKETINNLANLAKAEQQILFRIAQRDTQLDAWKTGFNNRVRK
                     GAGLLDASNIPITINGKTIKPVQAISLKGAPVYSGVSEQEIFALYRQMTGQNPNFRVL
                     PDGRLANGIISTGEWAGTKIALRNFSKTENSTQARWTLDLQNPPSFIKGTKLELKFQ"
     gene            499..873
                     /locus_tag="WGO49_001662"
     CDS             499..873
                     /locus_tag="WGO49_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0306747.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
     gene            1258..2688
                     /locus_tag="WGO49_001663"
                     /pseudo
     CDS             1258..2688
                     /locus_tag="WGO49_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
     gene            2707..2970
                     /locus_tag="WGO49_001664"
     CDS             2707..2970
                     /locus_tag="WGO49_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306748.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            3077..4444
                     /locus_tag="WGO49_001665"
                     /pseudo
     CDS             3077..4444
                     /locus_tag="WGO49_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
BASE COUNT         1607 a         1100 c         1261 g         1230 t
ORIGIN      
        1 gctgctccta aagaaacaat aaacaatttg gcaaatttag ccaaagcaga acagcagatt
       61 ttattccgta ttgcccaacg cgatacgcaa ctggatgcat ggaagacggg atttaacaat
      121 agagtaagga aaggagcagg cttgcttgat gcaagtaata ttccgataac cattaacgga
      181 aaaaccatca aacctgtaca agccataagc ttaaagggag cacccgttta cagcggcgta
      241 agcgaacagg agatttttgc gctttatcgg cagatgactg gccagaatcc gaattttaga
      301 gttttgcctg acggaagatt agcaaatggc attatcagta ctggagaatg ggcaggaaca
      361 aaaattgcat taagaaattt ttcaaaaaca gagaattcaa ctcaagcacg atggacatta
      421 gatttgcaga atcctccatc atttattaaa ggtactaaat tggagcttaa attccaataa
      481 tttacaaagg attttaccgt ggatgagaaa caaaaaatta agattcttga ttttcaaatc
      541 gatttatcct caatttttaa ctcttataaa aatcaaatgg gtattaatat tcaagataaa
      601 aacttaaaaa acaatttctg ttcttttatg gaagaactgt taaatgacgg ttcaatccgt
      661 ttacatgatt ataccgacgg tatcggaatt cctctaactg gaacttcaaa agaacaagtg
      721 cagaaattga aagacatatg gcctactttg gaagatgccc aagcaatatg gcctgaagac
      781 ccttggtatt acttagaatg gctttggtgg gatattgcgt gtccaataga tttggccgat
      841 ttgccgaata ttgatattta tgagcaagcg taggtatggt tagccgcctt tagcggcgta
      901 accgtacgca tatcagcaaa ctttataaaa taacaaggcc gtctgaaatc tgtttttcaa
      961 ctttttcaga cggccttgca acttggcatt tcattcgtac ggttacgccg ctaaaggcgg
     1021 ctaaccgtac ctacgagctc tgataaaaat gatttatgga agcaagctgt agcctgcatg
     1081 aaacctaaaa tccatgcgta aggtgtgtgc ttcagcgcgc acgcgttcca tgatttacgg
     1141 ctcaatgccg tctgaaaagc tcaatttttt cagacggcat ttgttatgca agtaaatatt
     1201 cagattccct atatactgcc cagatgcgtg cgtgctgaag acacccccta cgcttgctat
     1261 ttgaaacagc tccaagtcac caaagacgtc aactggaacc aggtacaact ggcgtacgac
     1321 aaatgggact ataaacagga aggcttaacc ggagccggag cagcgattat tgcgctggct
     1381 gttaccgtgg ttactgcggg cgcgggagcc ggagccgcac tgggcttaaa cggcgcggcc
     1441 gcagcggcaa ccgatgccgc attcgcctcg ctggccagcc aggcttccgt atcgctcatc
     1501 aacaataaag gcgatgtcgg taaaaccctg aaggagctgg gcagaagccg cacagtgaaa
     1561 aaactggttg tagcagcggc catcgcaggc gtatccaaca aaatcggtgc ttcctccctt
     1621 gccacttgga gcgaaacccc ttgggtaaac aacctcaacg tcaacttggc taatgcgggc
     1681 agtgccgcgc tgattaacac cgccgttaac ggcggcagtc tgaaagacaa tctggaagcg
     1741 aatatccttg cggctttggt gaatactgcg catggagagg cagcaagtaa aatcaaacaa
     1801 ttggatcagc actacattgc ccataagatt gcccatgcca tagcgggctg tgcggcagcg
     1861 gcggcgaata agggcaagtg tcaggacggc gcgattggtg cggtcgtggg ggagattgtc
     1921 ggggaggctt tggttaagaa taccgatttc agcggtatga ctgcttctga aattgaaaaa
     1981 gctaaagcga atattactgc gtatgcaaaa ttggtagccg gagcgactgt aggtgttaca
     2041 ggaggcaatg ttgatgtggc ggcaaatgct tccgaaacag ctgttaaaaa taatgcatta
     2101 gatattattt gggatatcgg caacctcgta tgggacggcg gtaaatggat ttacgccaaa
     2161 tctattggcg ataagcagat ggctcgagaa gcggcgattg attttggtgt ggatgccgcc
     2221 gcagctgccg ttccctttgt tccggcaggt gcgactaaaa tcagccgagg cggggcttat
     2281 gttctgaagg cgggagacga agcagttgat acggctaaag ccatacagga aattcagaag
     2341 cagaccggaa tcaagcttac ttatgataag gttaataagg tttggacaac accggcgggg
     2401 ttagattatg ggttagatgc taagcatggt aataggatta aacatgtttt agcccataca
     2461 attccaaatc caaacaaacc tgttcattct gtttttaatg tgtcccgtaa agaagttttg
     2521 cctttggttg atgaagcttg gagaatgaaa ggaaatcctt tgccaaatga ttcatccgta
     2581 tatcttgtag atatgaagaa acctattgga acaaaaggag aaacaaaagt gcggattgtt
     2641 gtgcaaaaag gaacaaataa aatcatttct gcatatcctc agaaataatt aagaaaggaa
     2701 tctcttatgg ataaagaaat taaaatttgc ccaagatgtg agcaaggcta cctttatcat
     2761 gcaaagccta aatatttctc tggggaggtc attttatgcg atgaatgtta tgctatgtgg
     2821 cttggggata tgaaaatttt ttacggacaa tatggaaaag atttttatga ttatcatgag
     2881 tttatgaaag ataaaggcat agaagaaata aatatgtggg aaggagagct ttttgatcac
     2941 ccatattatg aggatgaaaa atttaaataa ttgattttct gttccccgaa tttgggaaat
     3001 acgatgatat tttaaaccca aatattattt aaagtagcaa taggccgtct gaatatccgt
     3061 ttttcagacg gcctcaatgc aactgctggc agccgaaggc attcaccaac accaattgaa
     3121 tgttcagaaa agtacccgtt tcatcggcat caaagtgggt aaaagcaatt acagcaaaaa
     3181 cgagctgaac gaaaccaaac tgcccgtacg cgttatcgcc caaacagcca aaacccgttc
     3241 cggctgggat accgtactcg aaggcaccga attcaaaacc accctttccg gagccgacat
     3301 acaggcaggg gtgggtgaaa aagcccgagc cgatgcgaaa attatcctaa aaggcatcgt
     3361 taaccgcatc caaaccgaag aaaagctgga atccaactcg accgtatggc aaaagcaggc
     3421 cggaagcggc agcacggttg aaacgctgaa gctaccgagc tttgaagggc cggcactgcc
     3481 taagctgacc gctcccggcg gctatatcgc cgacatcccc aaaggcaacc tcaaaaccga
     3541 aatcgaaaag ctggccaaac agcccgagta tgcctatctg aaacagctcc aagtagcgaa
     3601 aaacatcaac tggaatcagg tgcagcttgc ttacgacaga tgggactaca aacaggaggg
     3661 cttaaccgaa gcaggtgcgg cgattatcgc actggccgtt accgtggtca cctcaggcgc
     3721 aggaaccgga gccgtattgg gattaaacgg tgcggccgcc gccgcaaccg atgcagcatt
     3781 cgcctctttg gccagccagg cttccgtatc gttcatcaac aacaaaggcg atgtcggcaa
     3841 aaccctgaaa gagctgggca gaagcagcac ggtgaaaaat ctggtggttg ccgccgctac
     3901 cgcaggcgta gccgacaaaa tcggcgcttc ggcactgaac aatgtcagcg ataagcagtg
     3961 gatcaacaac ctgaccgtca acctagccaa tgcgggcagt gccgcactga ttaataccgc
     4021 catcaacggc ggcagcctca aagacaactt gggcgatgcc gcactgggtg cgatagtcag
     4081 taccgtacac ggagaagtag cgagcaaaat caaatttaat ctcagcgaag actacattac
     4141 ccacaagatt gcccatgcca tagcgggctg tgcggcagcg gcggcgaata agggtaagtg
     4201 tcaggatggt gcgatcggtg cggctgtggg cgagatagtc ggggaggctt tgacaaacgg
     4261 caaaaatcct gccactttga cagctaaaga acgcgaacag attttggcat acagcaaact
     4321 ggttgccggt acggtaagcg gtgtggtcgg cggcgatgtg aatacagcgg cgaatgcggc
     4381 taaagtcgcg attgaaaata acctattatc tcaagaagag tatgctctta gagaaaaatt
     4441 gatcaaaaaa gccaaaggga aaggcctatt atctttagat tggggcagcc tgaccgaaca
     4501 agaggcaagg cagtttatct atttgattga gaaagatcga tattctaatc aattgcttga
     4561 ccgatatcaa aaaaatccaa gtagtttaaa taatcaagaa aaaaatattc ttgcatattt
     4621 tattaaccaa acctctggag gtaacacagc ttgggcagct tcgatactga aaacgcccca
     4681 gtcaatgggt aatctcacta ttccttccaa agatattaat aacaccttat cgaaagccta
     4741 tcaaacattg agtcgttatg attcttttga ttacaaatca gctgttgccg cacaacctgc
     4801 actttactta ttaaacggac cgcttggctt cagtgtcaaa gcagctactg tggcagcagg
     4861 aggatataac attggacagg gagcgaaagc aatctctaat ggagaatatc tgcatggtac
     4921 agttcaggtt gttaatggca cattgatggt tgcaggatct gtatctgcac aggctgcaat
     4981 atcggccaag cctgcacctg ttacccgtta tctgagcaat gacagtgctc ctgctttaag
     5041 acaagcttta actgctgaaa gccagagaat ccgcatgaaa ctgccggaag agtatcgaca
     5101 aatagggaat cttgcgatag caaaaattga tgttaaagga ttaccgcaaa ggatggaagc
     5161 atttagttct ttccaaaaag gggaacatgg atttattt
//
LOCUS       DAWFAD010000129         5160 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWFAD010000129 DAWFAD010000000
VERSION     DAWFAD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.129"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            407..5113
                     /gene="iga"
                     /locus_tag="WGO49_001666"
     CDS             407..5113
                     /gene="iga"
                     /locus_tag="WGO49_001666"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ0306749.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADKIKQRDNAGTIKGNGEHHWNITFG
                     TNSKIGSTAVRLAGNEKDANNGQNVTFEDNGTLVLDQNINQGAGGLFFKGDYTVKGIN
                     NDITWLGAGIDVTDGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVI
                     LNQQADADKKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFE
                     HIRNVDEGARIVNHNTSHASTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRR
                     PIPQGKDLYYKNYRYYALKSGGSVNAPMPENGVTENNDWVFMGYTQEEAKKNAMNHKN
                     NQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLS
                     GRPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKAAEIAVNQSASFSSGRNV
                     SNITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGN
                     VNLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNN
                     ASDAQSANQYHTLKINHLSGNGHFHYLTHLAENLGDKVLVKESASGHYQLHVQDKTGE
                     PNQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRV
                     KPVPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAE
                     AERKSAELAKQKAEAEREARELATRQKAEQERSSAELARRHEKEREAAELSAKQKVEA
                     EREAQALAVRRKAEAEEAKRQAAELARQQEEARKAAELAAKQKAETERKAAEIAEQKA
                     EAEREAAELAKQKAEEEGRQAAQSQPKRRNRRAIPPELSSDATTRALPRIARNSNPDA
                     SDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQAAAQPQ
                     AQAAAQPQAQAVAQADAVSTNTNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEK
                     NSVWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQ
                     ASGKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQKANFNRTSIQTGLTLGN
                     TLKINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLT
                     VKPLLSAAYFANYGKGGVNVGGNSFAYKADNQQKYSAGAALLYRNVTLNVNGSITKGK
                     QLEKQKSGQIKIQIRF"
BASE COUNT         1740 a         1211 c         1119 g         1090 t
ORIGIN      
        1 atccatccgt acggaaacct gcaccgcgtc attcccacga acccacatcc cgtcattacc
       61 acgaaagcgg gaatctagga cgcagggtta agaaaaccta catcccgtca ttccctcaaa
      121 aacagaaaac caaaatcaga aacctaaaat cccgtcattc ccgcaaaagc gggaatccaa
      181 aaacaaaaag ccgcagaaat ttaacggaag aaccgaaccc cgaaaatcgg cttcccgcct
      241 tcgcttgaat aacagaacaa aggaagcgga aataaaaaca tcgggattct ttttttaaat
      301 tggcaataaa acaccaaaat taatgattca cacaacaaaa aatagtcaac accgccaaac
      361 cggcgtaaaa tacaaaacat tatcaccctt aaaacggtaa aaccttatga aaaccaaacg
      421 ttttaaaatt aacgccatat ccttatccat ctttcttgcc tatgccctta cgccatactc
      481 agaagcggca ttggtcagag acgatgtcga ttatcaaata ttccgtgact ttgcagaaaa
      541 caaaggcaaa ttttttgtcg gcgcaaccga tttatcagtg aaaaacaaac aaggtcaaaa
      601 catcggcaac gcattatcca acgtaccgat gattgatttt agcgttgcag atgtcaacag
      661 acgtacatta accgtcatcg atccccagta tgccgtcagc gtcaaacatg taaaaggaga
      721 cgaaatctct tattacgggc atcacaatgg gcacttagat gttagcaatg atgaaaacga
      781 atatcgttca gttgcacaaa atgactacga accaaataaa aattggcatc acggtaatca
      841 aggtcgtctt gaagactaca acatggcacg ccttaataaa ttcgtaacag aagtcgcacc
      901 tattgcacca accagtgcag gcggcggcgt tgaaacctat aaagataaaa accgtttttc
      961 tgagtttgtg cgagttggag caggtacgca atttgaatat aacagccgct acaatatgac
     1021 agagctttca cgagcttatc gttatgccat tgcaggtacg ccttatcaag acgtgaatgt
     1081 cacatcgaat ctaaatcaag aaggcttgat tggcttcggt gataattcaa aacatcattc
     1141 cccagaaaaa ctcaaagaag ttttatctca aaacgcattg actaactatg ctgtgttagg
     1201 cgatagcggt tcaccattat ttgcttatga taaacaagaa aaacgctggg tctttttagg
     1261 tgcttacgat tattgggcag gctatcaaaa aaactcttgg caagaatgga atatctataa
     1321 aaaagaattt gcagataaaa tcaaacaacg cgataacgcc ggcaccatca aaggtaatgg
     1381 agaacatcat tggaacatta cttttggcac aaacagcaag attggttcta cagcggtaag
     1441 gcttgccggc aatgaaaagg atgcaaacaa cggacagaat gttacctttg aagacaacgg
     1501 cactttggta ttggatcaaa acatcaacca aggcgcgggc ggtctgtttt tcaaaggcga
     1561 ttacacagtc aaaggtataa ataatgacat cacttggcta ggtgcgggga ttgatgttac
     1621 cgacggcaaa aaagtcgttt ggcaagtcaa aaatccgaat ggcgacagat tggcaaaaat
     1681 cggcaaaggc acattagaaa taaacggcac aggcgttaac caagggcaat taaaagtcgg
     1741 cgacggtacg gttattctga atcaacaagc cgatgccgac aaaaaagtcc aggctttctc
     1801 ccaagtcggc attgtcagcg gacgcggtac attggtatta aatagttcaa atcagattaa
     1861 tcccgataac ctatatttcg gtttccgtgg cggtcgtttg gatgccaatg gcaatgactt
     1921 gacttttgaa cacatccgca acgtggatga aggcgcgcgc attgtcaacc acaacacaag
     1981 ccacgcctcc acaatcacgc taacgggtaa atctttaatt accaatccga acagcctttc
     2041 ggtacatagc atacaaaatg actatgacga agatgattat agttattact accgaccaag
     2101 gcgcccgatt ccgcaaggca aagaccttta ttacaaaaac tatcgttatt acgccctaaa
     2161 atccggcggc agcgtgaacg caccgatgcc cgagaacggt gtaacagaaa ataacgactg
     2221 ggtctttatg ggatatacgc aagaagaagc caagaaaaac gcgatgaacc acaaaaacaa
     2281 tcagcgtatt agcggtttta gcggtttctt tggcgaagaa aacggaaaag gacataacgg
     2341 tgcattaaac cttaatttca acggcaaaag cgcgcaaaac cgtttcttgt taacaggcgg
     2401 cactaattta aacggaaaaa taagcgtaac tcaaggcaat gtcttgttat caggtcgtcc
     2461 aacaccacac gcaagagatt ttgtgaacaa atcttcagcc cgaaaagacg cacatttctc
     2521 caaaaacaat gaagtcgtat ttgaagacga ctggataaac cgcacattca aagccgcaga
     2581 aattgcggtt aatcaatccg catcattctc ttccggtaga aatgtatcca acattaccgc
     2641 caacatcacc gcgacagaca acgccaaagt aaatttgggt tacaaaaacg gcgatgaggt
     2701 ttgcgtgcgc tcggactata ccggttatgt tacctgcaac acaggcaact tatccgataa
     2761 ggctttaaac agctttggag cgacacagat taacggcaat gtgaatctga accagaatgc
     2821 ggcattggtc ttgggcaagg ctgcattatg ggggcaaatt caagggcaag gaaacagccg
     2881 tgtcagccta aaccaacata gcaaatggca tttgactggc gacagccaag tacacaatct
     2941 gtcattggcg gatagccata ttcatttgaa caatgcctcc gatgcgcaaa gtgcaaatca
     3001 ataccacacg ctcaaaatca atcatttatc cggtaacggg cattttcatt atctgacgca
     3061 cttggcggaa aatcttgggg ataaagtgct tgtgaaggaa tccgcatccg gtcattatca
     3121 gctccatgtt caagataaaa caggcgaacc caatcaggaa gggctgaatc tctttgatgc
     3181 atcatccgta caagaccgct cccgcctttc tgtttccttg gcgaataacc atgtagattt
     3241 aggcgcattg cgttatacaa tcaaaacaga aaacggtatt acccgattgt acaatcctta
     3301 tgccgagaac cgccgccgag tcaaaccggt cccgtctcct gccacaaaca cggcttctca
     3361 agcacaaacg gacagtgcac aaattgccaa gcctcaaaat atcgtcgtcg caccgcctag
     3421 cccgcaggca aatcaagccg aagaagcaaa acgccaacaa gcaaaagcgg agcaagtgaa
     3481 gcgtcagcaa gcagaagcag agcgtaaatc ggcagagtta gcaaaacaaa aagcagaagc
     3541 cgaacgcgaa gcaagagagc tggcgacccg ccaaaaagca gaacaagaac gcagcagcgc
     3601 cgaacttgca cgtcgacatg aaaaagaacg cgaagcggca gagttgtcgg caaaacaaaa
     3661 agtcgaagcc gaacgtgagg ctcaagcatt ggcggtacgc cgaaaagcag aagccgaaga
     3721 ggcaaaacgc caagctgcag agcttgcacg ccaacaagaa gaagcacgaa aagctgcaga
     3781 gttggctgct aagcaaaaag cggaaactga acgcaaagca gcagaaattg ccgagcaaaa
     3841 agcggaagcc gaacgtgaag ccgccgaact tgccaagcaa aaggcggaag aagaaggtcg
     3901 ccaagcagca caatctcaac ccaaacgtcg taaccgcagg gctataccac cggaactgtc
     3961 atctgatgca acaacccgag ctttgcctag gatagcgaga aattcaaatc ctgatgccag
     4021 cgattatgaa gaaatcccct tggatgcgtt ggaagatgaa gatgtatccg aatcggttga
     4081 cacatcagac aaacagcctc aagacaatac ggaacttcat gaaaaagttg agacagtgag
     4141 tttgcaacca agagccgcgc agccgcgagc tcaagccgcc gcgcagccgc aagcccaagc
     4201 cgccgcgcag ccgcaagccc aagccgtcgc gcaagccgat gcagtcagca ccaataccaa
     4261 ctcggcttta tctgacgcaa tggcaagcac gcaatctatc ttgttggata caggtgcttc
     4321 attaacacgg cacattgcac aaaaatcacg tgctgatgcc gaaaaaaaca gcgtttggat
     4381 gtcgaacatc ggttatggcc gtgattatgc ttccgcacaa tatcgccggt ttagttcgaa
     4441 acgcacgcaa acacaaatcg gcattgaccg cagcttgtcc gaaaatatgc agataggcgg
     4501 cgtattgact tactctgaca gtcagcatac ttttgatcag gcgagcggca aaaatacttt
     4561 tgtgcaagcc aacctttatg gtaagtatta tttaaatgat gcttggtatg tggccggcga
     4621 tattggtgca ggcagcttga gaagccggtt acaaacacag caaaaagcaa actttaaccg
     4681 aacaagtatc caaaccggcc ttactttggg caatacgctg aaaatcaatc aattcgagat
     4741 tgtccccagt gcgggaatcc gttacagccg cctgtcatct gcagattaca agttgggtaa
     4801 cgacagtgtt aaagtaagtt ctatgtcagt gaaaacactg acggccggac tggattttgc
     4861 ttatcggttt aaagtcggca accttaccgt aaaacccttg ttatctgcag cttactttgc
     4921 caattatggc aaaggcggcg tgaatgtggg cggtaattcc ttcgcctata aagcagataa
     4981 tcaacagaaa tattcagcag gtgccgcgtt actgtaccgt aatgttacat taaacgtaaa
     5041 tggcagtatt acaaaaggaa aacaattgga aaaacaaaaa tccggacaaa ttaaaataca
     5101 gattcgtttc taaaatacta aattcatagc aaaataaaat gccgtctgaa ctcaggcttc
//
LOCUS       DAWFAD010000130         5003 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWFAD010000130 DAWFAD010000000
VERSION     DAWFAD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.130"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            12..422
                     /locus_tag="WGO49_001667"
     CDS             12..422
                     /locus_tag="WGO49_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306750.1"
                     /translation="MVQTVFFYPKETIMSKDKNQITVGINREIIDTETDSSAEFHVIR
                     HVSLDYATGFNVVLLDSYVRKSTFERRGRSVGSMQLNIPGVPPRGTDVLDWAYQAVVA
                     AVEGGAVDAYGQPISANSLTGAELVYADLPEAAI"
     gene            486..911
                     /locus_tag="WGO49_001668"
     CDS             486..911
                     /locus_tag="WGO49_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306751.1"
                     /translation="MSEPIITPAAVYIFGGVVGAGYLLGMPVDAVVLGAVASAAVTMI
                     SEPKSRWMVVAYIVIGGLLGGAFAPPLTHFLIGEVVGSHPSLGEQRIQFAHAFAPVVV
                     GLLWQVLLRFARAVWPSFEKHADDIVEWLLNLLPWRPKK"
     gene            908..1183
                     /locus_tag="WGO49_001669"
     CDS             908..1183
                     /locus_tag="WGO49_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306752.1"
                     /translation="MKLLLILSAAPSLISAYLMICRLNGRKWSALSPEAWAYLAFLGG
                     AIYTFYIVFAYGLTPSLGKFMMDIAACVYFGSRSVRVVKWRHRHGLK"
     gene            1322..1795
                     /locus_tag="WGO49_001670"
     CDS             1322..1795
                     /locus_tag="WGO49_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0306753.1"
                     /translation="MQVTKNFSLRELTRSETARRAGVPNEPSPADMDNIYYTAQQLEK
                     IREYVGRPIIVTSCFRSERVNKLVGGSPTSAHRHGLAADCDASGMTSPAFAKLLIKMR
                     DEGALKFDQLILEFPERGDGAWVHIGFRRNSPQRNQILTATKKNGKTVYLPGLHP"
     gene            1807..2316
                     /locus_tag="WGO49_001671"
     CDS             1807..2316
                     /locus_tag="WGO49_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306754.1"
                     /translation="MDILLKYWKPVGVLLLIVLIFTAWHFDRAEKYRMGREAAAAEIS
                     NRLKDGYIEQAKQARSAEQKAAAAFAERQTKLEEEKQNAEKTVAAMRLELNRLRHYAA
                     AQNRNRNLPATTTAATASDGASDSQGWLLFGQCAEKYAGLAETADGHAADLREWQAYG
                     HAVSSQRAE"
     gene            complement(2369..2716)
                     /locus_tag="WGO49_001672"
     CDS             complement(2369..2716)
                     /locus_tag="WGO49_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0306755.1"
                     /translation="MYIPDKGDIFHLNFDPSSGKEIKGGRFALALSPKAFNRATGLVF
                     ACPISQGNAAAARSSGMISTLLGAGTETQGNVHCHQLKSLDWQIRKASFKETVPDYVL
                     DDVLARIGAVLFD"
     gene            complement(2718..2954)
                     /locus_tag="WGO49_001673"
     CDS             complement(2718..2954)
                     /locus_tag="WGO49_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEZ0306756.1"
                     /translation="MLRVQKWGNSAAVRLPADMLKQLDFKIGDALVAEVHNGELRVRA
                     ARRFRLADLLAEMEETPPRVEGWEILDDVGNEVV"
     gene            complement(3313..3804)
                     /locus_tag="WGO49_001674"
     CDS             complement(3313..3804)
                     /locus_tag="WGO49_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4760 domain-containing protein"
                     /protein_id="HEZ0306757.1"
                     /translation="MFWQLTVVSVTAVIALGTIFINKKTSKQKATLDVILNDYQDAQF
                     VEADNHISPYIRGTAVDDNNARIDLYEIYQNKGGQWEKERGHLLTVINRHEFYACAIN
                     SGVLDEDLFKRLHCTNFIKLWNAVSPLVMKIREEERKDTIFRELEILVALWKANPLKA
                     SDL"
     gene            complement(4242..4610)
                     /locus_tag="WGO49_001675"
     CDS             complement(4242..4610)
                     /locus_tag="WGO49_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEZ0306758.1"
                     /translation="MIDGELVALIHQTVLADEAGLKGRADMACLDGALSRIANWRQYE
                     NLEDIYEIAALYAQAIAKAHAFPDGNKRTALLTMLTYLDLQGISIAADQGLDDLIVSL
                     AAGETDFKQLAETLRRLDKE"
BASE COUNT         1230 a         1210 c         1235 g         1328 t
ORIGIN      
        1 aaaccgtctg aatggttcag acggtctttt tttatccaaa ggaaaccatc atgtcaaaag
       61 acaaaaatca aatcaccgtc ggaatcaacc gtgaaatcat cgataccgaa accgacagtt
      121 ctgccgagtt tcacgtcatc cgacatgtgt cgctggatta tgcaaccggc tttaatgtgg
      181 tgttgttaga cagctatgtg cgcaaatcga catttgaacg tcgcggacgt tcggtcggtt
      241 cgatgcagtt gaatattccg ggcgtgccgc ctcgcggtac ggatgtattg gactgggcat
      301 accaggctgt tgtagcagcg gttgaagggg gcgcggtcga tgcctacggt cagcctattt
      361 ccgccaacag cctgaccggc gcggagttgg tctatgccga tttgcctgaa gcagccatct
      421 gattcataac acggaaggtc ggctgaaaaa cagtttcaga cgaccttcgc ataagggaaa
      481 agaaaatgtc tgaaccgatt attacccctg ccgccgttta tatttttggc ggcgtggtcg
      541 gcgcaggtta tctgttgggg atgcctgtcg atgcagtcgt gctgggcgca gtggcttcgg
      601 cggcggtaac gatgataagc gagccgaaaa gccgctggat ggtagtggcc tatatcgtta
      661 tcggcgggct tttgggtggc gcttttgccc caccgctgac gcatttctta atcggcgaag
      721 tagttggcag ccatccgtcg ctgggtgagc agcgcataca gtttgcacat gcctttgccc
      781 ccgtcgttgt cggcttgttg tggcaggtat tgttgagatt tgcccgcgct gtctggccat
      841 cgttcgaaaa gcatgcagac gatattgtgg agtggctgct gaatcttctg ccgtggagac
      901 cgaaaaaatg aaattacttt tgattctttc tgccgcccca tcactgattt cggcgtactt
      961 gatgatttgt cgtctgaacg ggcgcaaatg gagtgcattg tcgccggagg cgtgggcgta
     1021 tctcgccttt ttaggtggag cgatctatac cttttacata gtctttgctt acggactgac
     1081 gccgagctta ggcaaattta tgatggatat tgccgcctgt gtatatttcg gcagccgttc
     1141 ggtacgggtt gtcaaatggc ggcatcggca tggtttgaaa tagtttgcct gaaaaaagca
     1201 aaacgccgca aagtatgcag cttcgcggcg ttttcgtatc aaaactttat acgcagaatt
     1261 attgagatag ccgcctgtcg tttaggcggt tttttatttg aaaagaacat ggagaaccga
     1321 aatgcaagtt accaaaaatt tcagtctgcg cgagttgaca cgcagtgaaa ccgcgcgtcg
     1381 cgcaggtgta ccgaacgaac cgtcgcccgc cgacatggac aacatttatt acaccgcaca
     1441 gcagcttgaa aaaatccgcg aatacgtcgg tcgcccgatt atcgtaacaa gctgcttccg
     1501 cagcgagcgg gtaaacaaat tggtcggtgg cagcccgaca tcggcgcacc ggcacggact
     1561 tgccgccgat tgtgacgcat cgggtatgac ctcgccggca tttgccaaac tgttaatcaa
     1621 aatgcgtgat gaaggcgcgc tgaaattcga ccagttgata ctcgaatttc ccgagcgcgg
     1681 ggacggcgca tgggtacaca tcggtttccg acgcaacagc ccgcaacgca accagatact
     1741 gaccgcaacc aagaaaaacg gcaaaaccgt gtatctgccc gggctgcatc cttgaggtcg
     1801 tctgaaatgg atattttatt gaaatactgg aagccggtag gcgtattgct gctaatcgtc
     1861 ctgattttta ccgcatggca tttcgaccgt gccgaaaaat accgcatggg acgggaggct
     1921 gctgctgccg aaatttcgaa tcgtctgaaa gacggctata tcgagcaggc aaagcaggcg
     1981 cgttctgccg agcagaaggc cgctgccgcg tttgccgaac gacaaaccaa attagaagag
     2041 gaaaaacaaa atgctgaaaa aactgttgcc gctatgcgtc ttgagcttaa ccgcctgcgc
     2101 cactacgccg ccgcccaaaa tcgcaacaga aacctgcccg caaccactac cgccgccacc
     2161 gcatctgatg gcgcgtcaga ttcccaaggc tggctattat tcggacagtg cgctgaaaaa
     2221 tatgcaggac tggcagaaac agccgacgga catgcagccg atttaaggga atggcaggca
     2281 tatggtcatg cagtaagcag tcagcgtgcc gaataagcaa ccgcccgaac ctgtaagaaa
     2341 agattacagg ttcgggcggt ttcagcattt aatcgaataa gacggcgccg atgcgcgcca
     2401 gcacatcgtc caatacataa tcgggtacag tttctttaaa agaagccttg cggatttgcc
     2461 agtccagaga tttgagctgg tggcagtgga cattgccctg cgtttccgtt cctgcaccga
     2521 gtaaggttga aatcatgccg ctgcttcgtg cagccgctgc attcccctgt gaaatggggc
     2581 aggcaaaaac caatcccgtt gcgcggttga atgcttttgg agacagagcc agcgcaaacc
     2641 gcccgccctt gatttccttg ccgctggaag ggtcgaaatt caaatggaaa atatcgcctt
     2701 tgtcgggaat atacatttca gacgacctcg ttgccgacat catccaagat ttcccagcct
     2761 tctacgcgcg gcggggtttc ttccatttcg gcaagcaagt ctgccaagcg gaaacgtcgg
     2821 gcagcacgca cacggagttc gccgttatgt acttccgcta ccaaagcgtc gccgatttta
     2881 aaatccaatt gtttcagcat gtcggcaggc agtcggacgg ccgccgagtt cccccatttt
     2941 tggacacgca acataatctt cacctttatt gtatctacaa agtagataca tattaccata
     3001 aaatttcagt tgttcaaata cttgtgcaga atacgccaaa agccgtccga actgtttcgg
     3061 acggcttttg tactgtattt gcgccttcag gcaatatttt gttatccatt ttcaagatgc
     3121 aaaagctttc taattgcttg atgtcggatt tcggttgttt agggatacaa aaccaagtaa
     3181 actaaaactg tttgattgga aaatgctccg caaggagaaa attatgttca aaaaatcact
     3241 ttataaggct gctttggcgt atttcggcga ttgcgtggct gcccatattt cagaacagtt
     3301 ttgactgttt attcacaaat cagatgcctt tagggggttt gctttccata atgcaaccaa
     3361 aatttccaac tctctaaata ttgtgtcttt gcgttcttct tcgcgtattt tcataacaag
     3421 aggcgaaact gcattccaca attttatgaa gttggtgcaa tgcagccgtt taaacaaatc
     3481 ctcatccaat actcccgagt tgattgcgca cgcataaaac tcgtgccgat tgattacggt
     3541 aagtaaatgc cctctctctt tttcccattg tccgccctta ttttgataaa tttcatacag
     3601 gtcgatccgc gcgttgttgt cgtcaactgc cgtgccgcga atataaggcg aaatatgatt
     3661 gtcggcttct acaaattgtg catcttggta atcattcaaa ataacatcta atgtcgcctt
     3721 ttgctttgaa gttttcttat tgatgaatat tgtccccagt gcaatgacgg cggttactga
     3781 aacaacggtc agttgccaaa acatcagcca ttcggccgtc ccccataggt taaactgtac
     3841 gctgtgcctg aatatgtaaa ataaagtgaa tacagataca aacaaggagg ttagggcaat
     3901 tactgacaac caacctcctt gttttatttg tttccgcacc ttaccagcca ttaaaaccct
     3961 catttttgta tttcatcgta tttccttaat ggttgtaacc ttgcttgtcg atatctgtat
     4021 attaatgccg tgcgcccgtt ctttcaaggg gtgatttaaa aatcaggcat ccttgacatc
     4081 ctctcctgct taaaggcggg gggactcctg ccgtgtaaac aaatgccgtc tgaagggctt
     4141 tcagacggca taaaaaaacc gcctttgtgt aaagcggttg caaaaaagcc tttcaataaa
     4201 aatgccgtct gaatttcaga cggcattgtt gtcggatatg cctattcctt atccagccgg
     4261 cgcagggttt cggcgagctg tttgaagtcg gtttctcccg ccgccaaact gacaatcaag
     4321 tcgtcaagcc cttggtcggc ggcaatgctg atgccctgca aatccagata ggtcaacatt
     4381 gttaaaagcg cggtgcgctt gttgccgtcg ggaaaggcgt gggctttggc tatggcttgt
     4441 gcatagaggg cggcgatttc gtagatgtcc tcaaggtttt catactgccg ccagttggca
     4501 atccgcgaca atgcgccgtc caagcacgcc atatccgccc gccctttcaa acccgcttca
     4561 tccgccaata cggtttgatg gataagcgcg accagttcgc cgtctatcat ttgtcggcaa
     4621 gtgccttgac ggctttttga tgggttttgg caatgcggcg ggcggcggca agcagtacgc
     4681 gtttgcctgc ttcgccttta agctcgattt tgacggggcg tgtattttgg ttttgcatcg
     4741 ctgctccttg tctgtttgcg ggcattttag cttttttccg gcagcttggg aaatgccgtc
     4801 cgaaaacact tcagacggca ttcttctaat agtgtaatgc aatagttact ccaagatttt
     4861 tgtatatggc tgtttttatg gtaaattaaa ttttcataag aaataaatat atttaaatca
     4921 atgaaataaa atttagtcga atcccaccgt ttccgccaga aaacataacc gccctgaatc
     4981 agggcggttc ttttttgttc aag
//
LOCUS       DAWFAD010000131         4906 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWFAD010000131 DAWFAD010000000
VERSION     DAWFAD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.131"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(90..641)
                     /locus_tag="WGO49_001676"
     CDS             complement(90..641)
                     /locus_tag="WGO49_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306759.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDLEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFQ
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(695..1366)
                     /locus_tag="WGO49_001677"
                     /pseudo
     CDS             complement(695..1366)
                     /locus_tag="WGO49_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1627..3099
                     /gene="pyk"
                     /locus_tag="WGO49_001678"
     CDS             1627..3099
                     /gene="pyk"
                     /locus_tag="WGO49_001678"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0306760.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     LLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            3407..4807
                     /locus_tag="WGO49_001679"
     CDS             3407..4807
                     /locus_tag="WGO49_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0306761.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT         1313 a         1472 c         1145 g          976 t
ORIGIN      
        1 aaagaaaccg ccattagcac tacacccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgccg gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggt agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatctgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccaaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaactttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaataaggg gctgtcctag ataactagga caaacttgat tttactaatt
      721 gttttaaaat ggaacaagaa cttttatctc actgttgtta aaacgtcgtt cgcactcctt
      781 taaatacagc tcaaaatgcg ctttgggaat gccgttaaac ttgcgtaaat gacgttttgc
      841 ctggttccaa aagttctcaa ttccattaat atggttttgt cgttcagcaa aatgtgtgct
      901 gtgattgata cgaaaacgaa gtttcagcga agctaaaatg gctaaattcg cgcacatcta
      961 atacatcata gctacgataa caatccgtat aaacaatact gtcaggtttc acttgttcac
     1021 ggataatagg aaataaagta gcggtttgag tattcggtac tgtaaccgta taaaccttac
     1081 catttcgctt caaaagaccg aatacggcga ctttaccggc agcaccgcga ccgcgtttgc
     1141 ctttgcgttg tccgccaaaa taactttcat ctgcttctac ttcgccatca aacatttcca
     1201 aatgcggact gttttgataa ataagtaatc gtaaacgatg aaaataatag gctgcggtat
     1261 ttttattaac gcctactaac tctgctgccg ttcttgcagt tacacctgtg acaaacagtt
     1321 caatgagttt attttgttta tactggctta gacgactttt tctcataggg ataattctaa
     1381 cttaatttga atttccctag ttatctagga cagtcccaaa aataaattta gcctaagtca
     1441 taaaaccgtg taattttgca ccaaaaaagg cggtttgcag gtaaaatatc aaactattaa
     1501 tttaaatcat atttttacag aatattccgc cgcgttcatc aaatggacat caaaccggtt
     1561 ccaaatttct gtaattttgt aacaaaatac cgcaaaacac ccgattgaga ccaaaaggac
     1621 tttcatatga accagacaag ccgcgatctg acccgcatca gccacaacac caaaatcgtc
     1681 gccacccttg ggccgggcag caacaacgtc gaactgttgg aagatatgat ccgcgtcggc
     1741 ggcctgaacg tcgtccgctt caacttcagc cacggcacgc ccgaattcca tcaggaaaac
     1801 gcccgcatcg tgcgcgaagc ggcaaaacgc gccggacagg aaatcgccat cattgccgac
     1861 ctgcaagggc cgaaaatccg cgtgggcaaa atcgccggcg gcggcatcga attgaacaaa
     1921 ggcgaaacgc tgctactgga tgccgcgctt gaaggcgaag gcacgcgcga ggcggtcggt
     1981 ttggactacc gcgacctgcc cgatgacgtt gccgcaggcg atgtcttgtg gctggacgac
     2041 ggcctgctga ccctgaccgt ggaatccgtc gaaggcagca ggattatcac aagggtggaa
     2101 aacagccacg tcctgaaaag caacaagggc atcaacaaac gcggcggcgg tctgtccgca
     2161 ggcgcgttga ccgaaaaaga cttccgcgac ctgaaaaccg cgattgccat cggttgcgac
     2221 tacctcgcca tcagctttgt gaaatccgcc gaagatttgc atatcgcgcg cgccaaagtc
     2281 gaagaagaaa tgaaaggcag cacggccgtg cgccccggtt tggtttccaa aatcgaacgc
     2341 gtagaagcga ttgaaaactt ggacgaaatc atcctcgccg gcgacggcat tatggttgcg
     2401 cgcggcgact tggcggtcga agtcggacac gccgccgtcc ccgccctgca aaaacggatg
     2461 atccgccgcg cccgcgagtt gcgccgcttc agcattacgg cgacgcaaat gatggaatcg
     2521 atgattacca accccgtacc gacccgcgcg gaagtcagcg atgtggcaaa cgcggtattg
     2581 gacggtaccg atgcggtgat gtgttccgcc gaaaccgccg tcggcgcgta tccgtttgaa
     2641 accgtcagcc aaatggcaat tatctgcgcg gctgcggaaa aagagcagga ttcgctcaac
     2701 ggcgttgccg aacaggcgga gtatcccgaa gcggtcagca ccaacctggc ggttgccggc
     2761 ggcgcggtca gcgtggcgcg cgcggttcac gccaaagcga ttgtcgccct gaccgaaagc
     2821 ggttcgaccg cctttgaaat cagccgccac aacatcaccc tgccgatttt cgcgctgacc
     2881 ccgagcgttt ccgcccaacg ccgtatggcg atgtaccgcg gcgtgcgccc gctgattttg
     2941 gcaaccagta ccgaccacga cacggcgttg aacgaagtcg aaaccatgct ggtggaacac
     3001 aacatcctgc attccggcga ccagtacatc atcaccagcg gttcgcaaat gcgcgaatcc
     3061 ggttcgacca acacactgga agtgctgcgc gtcaaataat ccgccctgag tggaaaatgc
     3121 cgtctgaagc cgatgcccga ggcttcagac ggcatttttt gcggcggcgc ggcggttcgg
     3181 gcaaactgaa aaaattgtgc aaatccggca acatcggata aaattgagta cctatactaa
     3241 agcgaaacaa ggcatttccg actgcctttt ttatagtgga ttaaatttaa accagtacgg
     3301 cgttgccttg ccgtactatc tgtactgtct gcggcttcgt cgccttgtcc tgatttttgt
     3361 taatccacta tatttatcca ccgtccgcct ttttacggaa accgaaatga ccccttccgc
     3421 actgaaaaaa accgtcctac tgctcggcac tgcctttgcc gccgcatccg cacaagcctc
     3481 cggctaccac ttcggcacac agtcggtcaa cgcgcaaagc acggcaaatg ccgccgccgc
     3541 agaagccgcc gacgcatcga ccatcttcta caaccctgcc ggcctgacca aactcgacag
     3601 cagccagatt tccgtcaacg ccaacatcgt gctgcccagc attcattatg aggcggattc
     3661 cgccaccgac tttaccgggc ttcccgtcca aggttcgaaa agcggcaaaa tcaccaaaac
     3721 cacggtcgcg ccccacatct acggcgcata caaagtcaac gacaatctga ccgtgggctt
     3781 gggcgtgtac gtccccttcg gctctgccac cgaatacgaa aaagattccg tgttgcgcca
     3841 caacatcaac aaactcggtc tgaccagcat cgccgtcgaa cctgtcgccg catggaaact
     3901 caacgaccgc cattccttcg gcgcaggcat catcgcccaa catacttccg ccgaactgcg
     3961 caaatatgcc gactggggga ttaagagtaa agcagagata ttgacggcaa aaccgcccaa
     4021 acctaacggt gtagccgaag ctgcaaaaat tcaggccgac ggacacgccg atgtcaaagg
     4081 cagcgattgg ggcttcggct accaactggc gtggatgtgg gacatcaacg accgcgcgcg
     4141 cgtgggcgtg aactaccgtt ccaaagtctc gcacacgctc aaaggcgatg ccgaatgggc
     4201 ggcagacggc gcggcggcga aagcaatgtg gagtacgatg cttgcagcaa acggctacac
     4261 ggcgaatgaa aaagcccgcg ttaaaatcgt tacgcctgag tctttgtccg tacacggtat
     4321 gtacaaagtg tccgataaag ccgacctgtt cggcgacgta acttggacgc gccacagccg
     4381 cttcgataag gcggaactgg tttttgaaaa agaaaaaacc atcgtcaacg gcaaatccga
     4441 ccgcaccacc atcaccccca actggcgcaa cacctacaaa gtcggcttcg gcggttctta
     4501 tcaaatcagc gaaccgctgc aactgcgcgc cggcatcgct tttgacaaat cgcccgtccg
     4561 caacgccgac taccgcatga acagcctgcc cgacggcaac cgcatctggt tctccgccgg
     4621 catgaaatac catatcggca aaaaccacgt cgtcgatgcc gcctacaccc acatccacat
     4681 caacgacacc agctaccgca cggcgaaggc aagcggcaac gatgtggaca gcaaaggcgc
     4741 gtcttccgca cgtttcaaaa accacgccga catcatcggc ctgcaataca cctacaaatt
     4801 caaataaacg gcccgccgtt tgattgtaga aatgccgtct gaaacagatt tccgtttcag
     4861 acggcattgt tttaggcgcg atttatagtg gattaacaaa aatcag
//
LOCUS       DAWFAD010000132         4857 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWFAD010000132 DAWFAD010000000
VERSION     DAWFAD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.132"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(210..932)
                     /gene="rmpM"
                     /locus_tag="WGO49_001680"
     CDS             complement(210..932)
                     /gene="rmpM"
                     /locus_tag="WGO49_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ0306762.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPVVVVEQAPQYVDETISLSAKTL
                     FGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYVVA
                     NNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPDRR
                     VDVKIRSIVTRQVVPAHNHHQH"
     gene            1296..2246
                     /locus_tag="WGO49_001681"
     CDS             1296..2246
                     /locus_tag="WGO49_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ0306763.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(2359..3093)
                     /gene="fnr"
                     /locus_tag="WGO49_001682"
     CDS             complement(2359..3093)
                     /gene="fnr"
                     /locus_tag="WGO49_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ0306764.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTYFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            3298..4719
                     /gene="hemN"
                     /locus_tag="WGO49_001683"
     CDS             3298..4719
                     /gene="hemN"
                     /locus_tag="WGO49_001683"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ0306765.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTASV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
BASE COUNT         1241 a         1330 c         1173 g         1113 t
ORIGIN      
        1 gcggcaatgg gacacaaacc tgaatctgaa tccgaacctg caccccgatt ctgacgatag
       61 ccgtttcaga aaaccaccca aaaaaacaaa ccccgcaaaa gcgggggttt gtttcacatc
      121 atcgatgccg accgacgttt cacgaatatt gcaacagtac ctggtacaaa atccactaac
      181 ctcatgcatc ggcaagatat tgcctagcct tagtgttggt gatgattgtg tgccggcaca
      241 acctgacggg ttacgatgct gcggattttc acatccacac ggcggtcagg ttcgatacat
      301 gcaatcagag cctcacgttt tttggcttta gagactttcg cacccagttt ggcaacttcg
      361 gcttcacaaa cttgagtcat ttgcgcttga gattcgccca agccgacagc agaaattcta
      421 gaaacaggta cgccgttgct gaccaggttg tttgccacta cgtatgcgcg gcgttcggac
      481 agggcctgat tgtatttgtc agaacccata aagtcggtat ggccttcgac gcggacagat
      541 tggatattgg tttgacccag gcgttgcgcc aatactttca ggttgtcttg agcttcggcg
      601 cgcaatgaat ccttatcgaa accgaacagg gttttggcag acagggaaat ggtttcatca
      661 acatattgcg gagcctgctc cacaacgacg acaggagcgg gttcgggttc tggctcgggt
      721 tcgggggcag caaccgcatc gccgcattct acgcgacctt ggcttgcttt atcaaagtag
      781 gcgtttttcc agcattcgcc atagttgttg cgtacgattt cgttcgactg gccgcttacg
      841 gtgtaaccct gaacggacgc ctcgcccgca acagcagtgc cggaagcgag caatgcaacg
      901 aataatgcgc ttaatttcag ctgtttggtc attttattcc ctcattaaat ttgtacagca
      961 gatacagaag caactgctga attgccaaca tacaggccgc tgtagaaaca tggcggcaaa
     1021 tcggatattt cagtctccac tataaagaag cagggggagg actgtcaata ctcaacgcct
     1081 gaaaacaacc gtaaaacagc ctgtttcctg atagtctatc atgccgaaaa atcaattccg
     1141 tgtcattact ttagggggat tttttccata tcgcacgatt gccgttgcac aaaaacaaca
     1201 aacacaacgg caaagcccca taccgtaccg gcaagaaaat atgataaatt ataaacaatg
     1261 ttcagccacc cgacacagac ccacaccgac ccgccatgaa attacaacaa ttgaaatacg
     1321 ccttagaagt ttaccagcac aacctgaacg tttccgaagc ggccgaagcc ttgtttactt
     1381 cgcaacccgg catctccaaa caaatcaaat tgctggaaga agaaatcggc attcagattt
     1441 ttatccgcag cggcaagcgc gtggtttcgg tctcgcagcc gggcaaggtg gttttggata
     1501 ttgcggaacg tattttacgc gatgtacaga acattaaaaa tatcggcagc gagtttaccg
     1561 gacaggacag cggttcgctg acggttgcca cgacgcatac tcaggcacgc tatgccctac
     1621 ccttgattgt tgccgatttt gtgaaacgct atccgaaagt caacctgacc atcaaacagg
     1681 ggagccctgc cgccatcgca cgtatggtta cctcaggcga ggcggacttg gcgattgtta
     1741 cggaacggat agacgaccat cccgaactgg gaaaacttcc ctgctatgac tggactcatg
     1801 cggttatcgt accgaacgac caccccttgc tcgaatgcag aaaccccctc cgcattgaag
     1861 atttggcgag gtttccgctg attacttatg aatttgcatt caatgcgggc agcagcatcg
     1921 cgcgggcatt ttccaaagcc cgtttggaac agccggatgt cgcattggcg gcggcggata
     1981 cggacgtatt gaagacttat gtgcgcttgg gtttgggcgt gggactgatg gcgaaaatgg
     2041 cgtacaaccc ggatacggac ggcgatttgc agcttgtgga tgcggcacac ctgttcgagc
     2101 cgtcgccgac gtggattgct ttgcgcagcg atacttattt gcgcggatat gcctacgact
     2161 ttatccaagc gtttgcgccg cacctgacac gcgagaaggt ggataggatt ctgtacacgc
     2221 ccatcagcga ggatttttcg atttaggcgg ctgccggttt tcagacggca ctttgcggca
     2281 gatacaacaa acaggacaga tgttttcgtc tgccctgtgt ttattgagaa tgccgtctga
     2341 aatgttcgta cgggttaatc aaatggcgtg cgaacaaccg gataccatct tcttcaacac
     2401 ctgcagattg aggatcttga tgtgtttgtg ttcgacggaa atcaatcctt cctgatgaaa
     2461 tttagataat gtgcggctga cggtttcaag tttcagcccg agataactgc cgatttcttc
     2521 gcgggacatt cttaagatga agtcgttggc agcaaaacct cgggaataaa ggcgttggga
     2581 aaggttcaac agaaaggcgg caatccgctc ttcggcgcgc atattgccca acagcagcat
     2641 aacaccttgg tcgcgcacga tttcacgact catcatgcgg aaaaagtagg tacgcaggct
     2701 ggggatattc tgccccagtt cttcgatgtg ggtaaacggc agttcgcaca cttcgctgtc
     2761 ttccaaggcg accgcgtcgc aactgtgcac atgggaacag atgccgtcca tgccgatgag
     2821 ttcgcccgac ataaagaaac ccgttacctg atcgcggccg tcctgactgg cgacggttgt
     2881 tttgaagaag cccgaacgga tggcaaagag cgaggtaaag gcttcgccga cacagaacag
     2941 gtattcgccc tttttcaggc ggcggctttg acggatgacg gcatcgagtt ggctgagctc
     3001 gttgggcagc agcccgacag gcaggcagag ttcccgcaaa gaacaggaag aacacagcgt
     3061 tttcatctga tgtgtagtat tatgcgaagc cataccgtac ctttttgtgc gctttgcccc
     3121 atcatgatta tagtggatta aatttaaacc agtacggcgt tgcctcgcct tgtccttatt
     3181 taaatttaat ccactatatg tgcttattga cacatatcaa gacaggttta tcatactgtg
     3241 gcattctacc aaactatcca aaatttgact aacatcataa aaccgcacac acacaccatg
     3301 aaaatcattc agatacagaa caatcacaat gtaaacgatg accgccccga gtttgaccgc
     3361 gcgctgattg ccagcctgcc cgccagcggc ccgcgctaca cttcctaccc taccgccgac
     3421 cgtttccatg acggtttccg cgaaggcgaa tatatcaaag ctttacattt gcgcggtatg
     3481 ggcgcgttaa acaaaccgct ttccctttac attcacattc cgttctgcaa caccatctgc
     3541 tactactgcg gctgcaacaa aatcatcacc aaagacaaaa gccgcgccga tgcctacatc
     3601 gaatatcttg aaaaagaaat ggaactgctc gctccacatc tgaacggacg gcaccagctt
     3661 gcccaactgc acttcggcgg cggcacgccg acctttttga gcgacgaaca gatcgaacgt
     3721 gtcttccgca tgatacgcaa acatttcgag ttaatcccca ccggcgaata ctccatcgaa
     3781 atcgacccgc gcaaagtcag ccgcgacacc gtcctcatgc tcggcagact cggcttcaac
     3841 cgcatgagca tcggcattca ggatttcgac cccaaagtgc aggcagcggt caaccgcatc
     3901 caaagttacg aagaaaccaa agaagtcatc gatgcggcgc gcgaagcggg gttcaaatcc
     3961 gtcagcgtcg atttgattta cggcctgccg caccagactt cggaaagcat caaaaccacc
     4021 atcgataccg ttttgtcgct cgatcccgac cgcctcgccc tttatcacta cgcccacctg
     4081 ccgcacgtgt tcaaaccgca acgccgcatc gataccgcct ccgtccccga cagcgaagag
     4141 aagctcgata tgctgcaata ctgcgtccaa accctaaccg aacgcggcta cgtcttcatc
     4201 ggcatggatc atttcgccaa acctgacgac gaactctcca tcgccctcaa agaaggcttc
     4261 ctccagcgca acttccaagg ctattcgacc tacgcggatt gcgatttggt cgccatcggc
     4321 gtgtcgtcca tcggcaaaat cggcagcacc tattcccaaa acgaacgcga catcgatgcc
     4381 tactatgccg ccatcgacga aggcagactg cccatcatgc gcggttatca gctcaatcag
     4441 gacgacatcc tgcgccgcaa tatcattcag gatttgatgt gccgcttcgc gctcgactac
     4501 cggatttacg aaagtatgtt cggcatcccg ttcgaccgct acttcaaaga cgaactggcg
     4561 gatttggaaa aactcgccgg tttgggattg gtgcgcctga acagccacgg gctgaccgtt
     4621 accccgaaag gacgcttcct catccgcaac atcgccatgg tgttcgacta ccacctgcgc
     4681 cacaaggaaa ccaaggcgaa atattcgcaa acggtgtgat tgcgtctaac gtacaaatgc
     4741 cgtctgaaag gctttttcag acggcatttt gctgccggca ggataagtgt tttcaagaac
     4801 aggcggcggc atatcataac gttccgcacc tttgtgtccg accgttccga aaccaag
//
LOCUS       DAWFAD010000133         4838 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWFAD010000133 DAWFAD010000000
VERSION     DAWFAD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.133"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            97..903
                     /locus_tag="WGO49_001684"
     CDS             97..903
                     /locus_tag="WGO49_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ0306766.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            complement(961..2226)
                     /locus_tag="WGO49_001685"
     CDS             complement(961..2226)
                     /locus_tag="WGO49_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ0306767.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDGTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLNPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEAIIKEFQTKGFITNE
                     AGYGKEITNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            2633..3343
                     /locus_tag="WGO49_001686"
     CDS             2633..3343
                     /locus_tag="WGO49_001686"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ0306768.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPKTE
                     AGLALLKSLGIPLAVVTNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKDEE"
     gene            3416..3877
                     /gene="recX"
                     /locus_tag="WGO49_001687"
     CDS             3416..3877
                     /gene="recX"
                     /locus_tag="WGO49_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ0306769.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IDVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            3951..4112
                     /locus_tag="WGO49_001688"
     CDS             3951..4112
                     /locus_tag="WGO49_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306770.1"
                     /translation="MSEQPEKNHNPLLEDERKNPVYRMGQAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            4265..4747
                     /locus_tag="WGO49_001689"
     CDS             4265..4747
                     /locus_tag="WGO49_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0306771.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
BASE COUNT         1273 a         1417 c         1101 g         1047 t
ORIGIN      
        1 aaagacagtt ttcagacagc aaatccgtct tcacacgata cctattttgt tataacataa
       61 caaaatcttt gacccacacg agacaaaggc tgcaccatga agaaaacatt gacactgctc
      121 gccgtttccg ccctatttgc cacatccgcc cacgcccacc gcgtctgggt cgaaaccgcc
      181 cacacgcacg gcggcgaata ccttaaagcc gacttgggct acggcgaatt tcccgaactc
      241 gaacccatcg ccaaagaccg cctgcacatc ttcagcaaac cgatgcagct ggttaccgaa
      301 aaaggcaagg aaaacatgat tcaacgcggc acatacaact accagtaccg aagcaaccgt
      361 cccgttaagg acggcagtta cctcgtcatc gccgaatatc agcctacttt ctggtcaaaa
      421 aacaaagcag gctggaaaca ggcgggcatc aaagaaatgc ctgacgcaag ctattgcgaa
      481 caaacccgaa tgttcggcaa aaacatcgtc aacgtcggac acgaaagcgc ggacaccgcc
      541 atcatcacca aaccggtcgg acaaaacttg gaaatcgtcc cgctggacaa tcccgccaac
      601 attcacgtag gcgaacgctt caaagtccgc gttctgttcc gtggcgaacc gctgcccaat
      661 gccaccgtta ccgccacctt tgacggcttc gacaccagcg accgcagcaa aacgcacaaa
      721 accgaagcac aggctttctc cgacagcaca gacgacaaag gcgaagtgga catcatcccc
      781 ttgcgccaag gcttctggaa agccaatgtc gaacacaaag ccgacttccc cgatcaaagc
      841 gtgtgccaaa aacaggcgaa ctactcgact ttaaccttcc aaatcggcca ttcgcaccat
      901 taatcccgcc cgcacaaaaa tgccgtctga aggcttcaga cggcattttt tgttcaaaca
      961 tcaataccaa ccgcgcagtt tcatcgcttt ttcaacacgg cggatactca tcatgtaaga
     1021 cgcggttcgc aaatcgacat catactcttg cgccaagttc catatatcgc ggaacgcacg
     1081 gcgcaggacg acggtttctt tttcctgaac ttcgtcaaac tcccaataat agccttgcag
     1141 attttgtacc cattcaaaat aagataccac cacgccgccg cagttcgcca aaatatcggg
     1201 cacgaccaat acgccgtttt gacgcaggat aacgtcggct tcgggcgtag tcgggccgtt
     1261 cgcgccttcg accacgattg tcgcgcggac tttaccggca ttttcagaag tcagttggtt
     1321 ttccagtgcg caaggggcaa gtacgtccac ttccagagcc aaaagttcgg cgttggtaat
     1381 ttccttgccg tagccggctt cattggtgat gaaacctttg gtttggaatt ctttgatgat
     1441 tgcttccata tccaagccgt tttcgttgta aatggcaacg tcaacagtag aaaccgcaac
     1501 aactttcgcg ccggattgat gcgcgtaata acctgtgtgg taaccgacat taccgaagcc
     1561 ttggatggcg taagtggcac ccttcacgtc tttgcccagt ttttccaagg cttggacggc
     1621 ggcgaggttc acgccgtaac cggtagcctc ggtacgcgcc aaagagccgc cgaactcaac
     1681 cggtttaccg gtaaatacgc ccggtgcaga atgtttcacc acgttttcat aggcatcaac
     1741 catccaagac atgattttgc cgttggtgtt cacatcaggt gcgggaatat cgattttctc
     1801 gccgatcagc ggagaaatgg cttcggaata agcgcgggcg atgcgttcca gttccgcctc
     1861 ggaataatcg cgcggattca aggtaacgcc gcctttgccg ccgccgtaag gaatacccgc
     1921 aacgcagcat ttgatggtca tccaaatcga cagggctttg acttcgtcca gattcactcc
     1981 gggatggaag cgcacgccgc ctttataggg gccgacggcg ttgttgtgtt gcgaacggta
     2041 gcccgtgaag gttttgaccg tgccgtcgtc gagtttgacg gggaaattga cttccaacac
     2101 gcgggtcgga ctcttcagga tttcataaac ggccggatcg gttttcagcc ggtcgcaggc
     2161 ggttttgacc tgtttgcgcg cgatttcaaa cggattgagg gtttcttttg caagggcttc
     2221 agacattttg cttccttttc acaaaaagat tttcaggggc gtgcatttcg ctcattacta
     2281 taaccaattt cccgacaaaa tgtaatagcg tgtagtcggc ataagcccga tttgattaat
     2341 ctatatatga ttttattttt caagccgcac ggaatcagcc cggactccgc ctgaaaaagc
     2401 agcgcgcgcg tccgaaaaat aaatgtcaaa ttaaataaag atataggcat ccttttattt
     2461 tttaaaaatt taattggaac atcgctggga tttcaaaccc ttgcccgatt ccgccccgaa
     2521 atccgaaaac accgcctgca aaacctgttg cgattgttaa caatctatac attagaagcc
     2581 ctgtgcaaac gatgttaaaa taaacccttt caacccgaca ggaaatcaga ttatgaatgc
     2641 agccatcgaa cacgtccaag ccgtcgcctt cgatttggac ggcacactgt gcgattccgt
     2701 ccccgacctt gccgccgccg cagaagcgat gttggagcaa ctcggtatga aaccgctgcc
     2761 tgcaaaagtg gtcgaaagct atgtgggcga cggcatcggc aaactggttc accgcgtcct
     2821 caccaacgac cgcgaccgcg aagccgattc cgaactgtgg gaaaaaggtt tcgtatccta
     2881 tatgaaatac taccgcgacc atttgagcgt cttcacccgc ccctatccca aaaccgaagc
     2941 cgggctggca ttgctcaaat ctttaggcat cccgctcgcc gtcgttacca acaaaaacga
     3001 aatccttgcc gtcgaactgt tgaaacagct cggacttgcc gactacttca gcctgatact
     3061 cggcggcgac agcctgcccg agaaaaaacc cagccccctg ccgctgcggc atgccgccga
     3121 agttttgggt atcgatgttg caaacatggt tatggtcggc gactcgcgca acgacatcat
     3181 cgccgccaaa gccgccggct gcctgagcgt cggcgttacc ttcggttacg gcgatatgac
     3241 gctgctctcg caagacgatg cgacccgccc cgactggatt atcggctcgc tgcccgaaat
     3301 ttacgaaaac ctgcaaccgc agaaaaataa agacgaagaa taaaacaatc tgttcagacg
     3361 gcatcggaat ccgtttccca tgccgtctga atctttccgt gccgaaaccg ccgccatgaa
     3421 accgcaaaaa tccctacgcg cccgcgcgat ggacatcctc tcgcgccaag aagtcagccg
     3481 catcggtctg aaacgcaaac ttgcaccgca cgccgaaagc gaagaagagt tggaaaacgt
     3541 gttaaacgaa tttgccgaac gcaactggca gtcggatttg cgctatgccg aagcctatat
     3601 ccgcagcaaa agccgcaaac acggttcatt gaggctgaaa caggctttgg cgcaacaggg
     3661 catagatgaa gaaaccagcc gcaacctgct tcccgaccgc tcaagcgaaa aacaggccgc
     3721 catagacgtg ttgcgtaaaa aattcaaaca ccccgccgcc gaccttaaag aaaaacaaaa
     3781 acaggcgcgt ttcctcgcct atcgcggatt cgatgccgat accgttcaga cggcattgaa
     3841 acacgcttgg gacgaaaatt gggaagacgg ctgctgacct tccttcttga atctttttgc
     3901 acggcaacat aaccttacgc ccgtttccaa cttttccgat tgagaacaaa atgtccgaac
     3961 aacccgaaaa aaatcataac cccctccttg aggacgaacg caagaacccc gtgtaccgca
     4021 tggggcaggc agttgccgga ttcatgctcg tcgtttgggt cgccgtattg gcactcgtgt
     4081 ttttcctggt cttccgtttt tggctttcct aaacaaaatg ccgtctgaag gcttcagacg
     4141 gcatcggcgg tccatttcgg cagactatcc catcatagct ttttttagct tgaattccac
     4201 ttccccattc cctaaaattt ccccgcaccc atttcaaaac accctttctt aaaacaggta
     4261 caccatgaca caacaacgcc aactgccttc ccacgaactc attatgtccg aactgatgat
     4321 gccggacacc gccaatttca gcggcaacgt acacggcggc gaactcctgc tcctgctcga
     4381 ccaagtcgcc tattcctgcg ccagccgtta cagcggcaat tattgcgtta ccctgtcggt
     4441 tgacaaagtc ctgtttaaag aacccatcca catcggcgat ttggttacct tttacgccgc
     4501 tgtcaactac acgggacgca cctcgatgga aatcggcatc cgtgtcgaag cacaaaacat
     4561 ccgcaccggc gaaatccgcc acaccaacag ctgctacttc acgatggttg cggtcaaaga
     4621 cggcaaaccc gtccctgtcc ctccgctgga aatcctgacc gaccgccaac gctgccgcta
     4681 cgaaaaagcc aaaaaacgca gagacatcag cctgcaagcc tctgaagaca tgtcctgcgg
     4741 ctgctgacgg cggactatgc cgtctgaaag acaggcacat cgcgccatcc gtttccattg
     4801 caaacggatg aaatcaagca aatatagtgg attaaatt
//
LOCUS       DAWFAD010000134         4776 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWFAD010000134 DAWFAD010000000
VERSION     DAWFAD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.134"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            214..993
                     /gene="pgeF"
                     /locus_tag="WGO49_001690"
     CDS             214..993
                     /gene="pgeF"
                     /locus_tag="WGO49_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ0306772.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDNPEAVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALDGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTTVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1045..1524
                     /locus_tag="WGO49_001691"
     CDS             1045..1524
                     /locus_tag="WGO49_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306773.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1526..2524
                     /locus_tag="WGO49_001692"
     CDS             1526..2524
                     /locus_tag="WGO49_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ0306774.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPSKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIG
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLREEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNSLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            2645..3064
                     /locus_tag="WGO49_001693"
     CDS             2645..3064
                     /locus_tag="WGO49_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306775.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAIIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3140..3655
                     /locus_tag="WGO49_001694"
     CDS             3140..3655
                     /locus_tag="WGO49_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0306776.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLYLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3739..4623)
                     /locus_tag="WGO49_001695"
     CDS             complement(3739..4623)
                     /locus_tag="WGO49_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ0306777.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGSQSLELNRDLVGQLAEINKDLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARKREGKKLGE
                     HLLQRLEAMEEIIDALSELFPTLLETHKEKIRARLVEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
BASE COUNT         1065 a         1262 c         1337 g         1112 t
ORIGIN      
        1 gccgtactgg tttttgttaa tccactatat aaagctcgga gaaggtatat aagggcatca
       61 ttcccgcacc tgcaaaaatc cgaacccgaa cgtttgaaaa caatcccgaa tctccgaatt
      121 cccgcctgtg cgggaatgac gaaaaaacaa acatccattt gccccgaagg cagttaatca
      181 accctttccg ccacacacct attccaatat ccaatgaaaa ccatcacaga aaccctaaat
      241 cttgccccga aaggcaaaaa cttcctgacc gccgattggc ccgcgcccgc caatgtgaaa
      301 accctgatta ccacgcgcaa cggcggcgtg agccaaggtg cgtatcagag tttgaacctc
      361 ggtacgcacg tcggcgacaa tcccgaagcc gtgcgccgca accgcgaaat cgtgcaacag
      421 caggtcggat tgcccgttgc ctacctcaat caaatccaca gcaccattgt cgtcaatgct
      481 gccgaagcat tggacggcac gcccgatgcg gacgcttccg tggacgacac gggcaaggtt
      541 gcctgtgccg tgatgaccgc agactgcctg cctgttttgt tttgcgacag ggcgggtacg
      601 acggttgccg ccgcacacgc gggctggcgc ggtttggcgg gcggcgtatt gcaaaacacc
      661 attgcagcga tgaaggttcc gcctgtcgaa atgatggcgt atctcggccc cgccatcagt
      721 gcggatgcgt ttgaagtcgg acaggacgtg tttgatgcgt tctgcacgcc catgcccgaa
      781 gccgccgccg catttgaagg cataggcggc ggcaaattcc ttgccgacct ttacgcgctc
      841 gcccgcctga ttctgaagcg cgaaggcgtg ggcggcgtgt acggcggcac gcattgtacg
      901 gttttggaac gggatacttt cttttcctac cgccgcgacg gagcgacagg gcgtatggcg
      961 agcctgattt ggctggacgg caatgccgtc tgaacacgcc gctgatataa tctaccgact
     1021 ttgtgctttt gagaaaggca agccatgaac aaactgtttc ttactgccgc agtgctgatg
     1081 ctgggcgcgt gcggtttcca cctgaaaggt gcagacggca tttctccgcc gctgacctac
     1141 cggagctggc acatcgaagg cggacaggca ttgcagtttc ctttggaaac cgcgctgtat
     1201 caggcttcgg gcagggtgga cgatgctgcc ggcgcgcaga tgaccctgcg tatagacagc
     1261 gtttcccaaa acaaggaaac ctacaccgtt acccgtgcgg cagtcatcaa cgaatatctt
     1321 ttgatattga cggttgaagc gcaggtattg aaacgcggcg agccggtcgg caaaccgatg
     1381 accgtgtccg tccgccgcgt ccttgcttat gccgacaacg agatcttggg caaacaggaa
     1441 gaggaagcgg cattgtgggc ggaaatgcgg caggatgccg ccgaacagat tgtccgccgc
     1501 ctgacctttc tgaaggcgga atgacgtggc ggcacatatc ggacgcattg atacggacgc
     1561 gcctttgaaa cccctgtacg tcatccacgg cgaggaagaa ctgttgcgta tcgaggcatt
     1621 ggacgcattg agggcggcgg cgaagaaaca aggttacctt aatcgggaag tttatacggc
     1681 agacaatgct ttcgattgga acgagctgct gcaaaccgca ggcagtgcgg gtctgtttgc
     1741 cgatttgaag ctgttggaac tgcatatccc taacggcaag cccagcaaaa ccggcggcga
     1801 ggcgttgcag gattttgccg cccgcctgcc ggaagatacg gtaacgctgg ttttactgcc
     1861 caaactggag aaaacccagc tccagtccaa atggtttgcc gcattggcgg caaaggggga
     1921 agtgtgggaa gccaaaccgg tcggcgcggc ggctttgccc caatggatac gcggacggct
     1981 ggacaaaatc ggtttgggta tcggggcaga cgcattggca ctgtttgccg agcgcgtgga
     2041 aggcaatctg ttggcggcac gtcaggaaat cgacaagctc gcgctgctgt atccgaaagg
     2101 gcataccgtc aatatcgatg aggcgcaaac cgccgttgcc aacgtcgccc gcttcgacgc
     2161 gttccaactg gcaggcgcgt ggatgaaggg cgatgtcctg cgcgtatgca ggcttttgga
     2221 cggattgcgg gaagagggcg aagaaccggt gctgttgctg tgggcggttg ccgaagacgt
     2281 gcggacgctg atccggcttg ctgccgccct gaagcagggg cagagcatcc aatccgtccg
     2341 caacagcctt aggctttggg gcgacaagca gacgctcgca ccgcttgcag tcaagcggat
     2401 ttccgtcgtc cgcctgcttg acgcgctcaa aacctgtgcc caaatcgacc gaatcatcaa
     2461 aggcgcggaa gagggcgacg catggacggt attcaaacgg cttgtcgtgt cgctggcgga
     2521 ataaagcggt aatccccaaa atccgaaaat actgtaaaat accgttaatc ctgaaaagta
     2581 ttcaccaatc cgtccgaaaa catttcagac ggcacgacca cctcaataaa ggaacattaa
     2641 ccctatggac aataagacca aactgcgctt gggcggcctg attttactga ccaccgccgt
     2701 tttaagcctc attatcgtat tgattgtcga ttcctggccg cttgccatcc tgcttgccgc
     2761 catcatcgtc gccgccgctg cgggcggttt tgtttggaca tcccgccgac agcaacgcca
     2821 gtttatcgaa cgcctgaaaa aattcgacat cgatcccgaa aaaggcagaa tcaacgaggc
     2881 aaacctgcgc cgtatgtacc acagcggcgg acaacaccag aaagatgcga ttaccctgat
     2941 ctgcctgtcg caaaaatgtt cggtggacga ggcgcacgct atgttcaaaa aacgcccgac
     3001 acgtcaggaa atcaatcaaa tggcggcaaa acagtcgcgc ggtcagaaac gtccgcaccg
     3061 ttaaccgccg caaggcatct ttgcataaat gccgtctgaa gcctgttggc gtttcagacg
     3121 gcatattctg attgaaaaga tgatgatact gaaaaccgcc ccgctcaaac gccgctttgc
     3181 cgccatgctg tatgagatgc tgctggtcgg tgcggcaacc tgtttggcag cattgtttgc
     3241 cggtattgcc gccatttttc tgaatcccgt ttctatcgcg gtttctgcat tggtaacgag
     3301 tatcctgata atgggatcat ggtggcttta ttttcgcgcc aactggcacg gtcaggggca
     3361 gaccttggcg atgaggacat ggaaaatcgg cttgtgcgac cttaacggca tacagccgtc
     3421 tttgtacctg ttgcgcctgc gctttatttg ggcgtgcata ttcatcgtct tcattcctat
     3481 gcttgcttat gccggattgc gccacttcct cggcattccg cccaagggcg cggccggcgc
     3541 ggcattgatt tggctgattt taccgtgggg gttcgcactg ctgaatcccg accggcagtt
     3601 tctgtatgat tttcttgcag gaacaagatt ggtggcggtc aaaggaaagc cttaagcctt
     3661 tataccgcaa aggtttcaaa ctgaaaaaat gccgtctgaa agggctttca gacggcattt
     3721 gcttatcggg gaaaccgatt attcgatatt ctgcacttgt tcgcgcatct gttcgatcag
     3781 gactttcagc tcgaccgagg cttgggtgca ttcggtagcg atggatttgc tgcccaaagt
     3841 gttggcttcg cggtttaatt cctgcatcag gaagtccagc cgtttgccgc tgctgccttt
     3901 gtgttcggta acgatacggc gcacttcggc aatgtgggtg cgtaggcggc tgaactcttc
     3961 gtcgatgtcg gatttttgga taaagagggc aaattcctgt tgcaggcggt cgttgtcgat
     4021 gctgccgacc gcttcgacga ggcgggcgcg gattttttct ttatgtgttt ccaacagggt
     4081 aggaaagagt tcgcttaatg catctatgat ttcttccata gcctcaaggc gttgcagcag
     4141 gtgctcgcct aatttcttac cttcccgctt gcgtgcggca gtaaagtctt ttaacgcttt
     4201 ttcggtcagt tcggtaatgc tttttgccaa ttcttccgta ttttcccttt ggcttgccaa
     4261 tacgccgggg aaacgcagga tgtcggcaac gcccagtttt gccaaatcgt gatgcttgcg
     4321 gaggtctttg ttgatttcgg caagctgtcc gaccaagtcg cgattcagtt ccaaggactg
     4381 actgccgttt tccgcatctt gaatttggat tttgcattcg actttgccgc gtgcgatatg
     4441 ggatgaaatt ttctcgcgga taccgctttc caaatagcgc aaatcgtcgg gcatcctgat
     4501 ttgaatatct agaaaacggt ggttgaccgc acgcaggtcg agattgatgc gtttgctgcc
     4561 gcactctgcc gccgcgttgg caaatccggt catgctgtgg atgtggatat ttccgctgct
     4621 catgtcgttc tccgaagccc gttaaaatgg aatcaatata tcacatctgt atggcggcaa
     4681 gcgttttcgg gtgtgaaaaa ttgaagattt tgcagcggca gattggaatc acgcgctttt
     4741 gttgctgcaa ggaagggaaa tgtatagtgg attaac
//
LOCUS       DAWFAD010000135         4768 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWFAD010000135 DAWFAD010000000
VERSION     DAWFAD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.135"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..478)
                     /locus_tag="WGO49_001696"
     CDS             complement(<1..478)
                     /locus_tag="WGO49_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0306778.1"
                     /translation="MKIILTTSMSGLGGTETATVRLGRLLKRRGHDIILASSDGPFVG
                     EAQASGIRWQPVDFYRGGLAGYLKSTFAYARMLRREQPDIIDCQMARVVPACALAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPAGRIAYA"
     gene            complement(475..1896)
                     /locus_tag="WGO49_001697"
     CDS             complement(475..1896)
                     /locus_tag="WGO49_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ0306779.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRHAPFSPAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEENAPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFW
                     LFFVFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAVYLAG
                     CILRHWKDLHKLFHYLKKQGFPL"
     gene            complement(2091..3200)
                     /gene="ribD"
                     /locus_tag="WGO49_001698"
     CDS             complement(2091..3200)
                     /gene="ribD"
                     /locus_tag="WGO49_001698"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ0306780.1"
                     /translation="MFSDTDISMMETALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDDSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERDEDKLRPYREHAHIRILMPSETAGGK
                     IDLNHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSSEILGHDIKTVFRKNDNAF"
     gene            complement(3233..3697)
                     /gene="nrdR"
                     /locus_tag="WGO49_001699"
     CDS             complement(3233..3697)
                     /gene="nrdR"
                     /locus_tag="WGO49_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ0306781.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPSCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTKHRLYTSGQRD
                     IPSAALADMVLKELLEHNTEAAVRFAALHKRFDNPADFASWLAQAVKTDRKA"
     gene            complement(3726..4568)
                     /locus_tag="WGO49_001700"
     CDS             complement(3726..4568)
                     /locus_tag="WGO49_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ0306782.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVIAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDESWSQLAVDELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
BASE COUNT          976 a         1223 c         1614 g          955 t
ORIGIN      
        1 aggcgtaggc aatccggccg gcggggaagc cgtggcggat gagtttttcg cgctcgtgtt
       61 tacagttgcc gatgatgtac acgcccagct tgtcgaagag cttggcgatt ttggggtagg
      121 ttgccgcgtc caaaccgtgc gagtggtaga tgattttggt tttcggcgaa acgattttgg
      181 cggcaagggc gcaggctgga acaacgcgcg ccatttggca gtcgatgatg tcgggttgtt
      241 cgcggcgcag catccgggcg taggcaaagg tgcttttgag gtagccggca agcccgcccc
      301 ggtaaaaatc aaccggctgc caacggatgc ccgatgcttg cgcctctccg acaaaaggcc
      361 cgtccgaaga agcaaggatg atgtcgtgcc cgcgccgttt gagcagccgc ccgaggcgga
      421 cggtggcggt ttccgtgccg cccaagcccg acatagaagt ggtcaggatg attttcataa
      481 tggaaaccct tgttttttca aataatgaaa cagtttgtgc aaatctttcc agtggcgcag
      541 gatgcagcct gccagatata ccgcccatac gccggcaaac agggggtagt ttgccggtgt
      601 gccgaagcag gtgtaggccg ccgaggaggt caggcagagc aatgtgtgcg tataaagcgg
      661 caggcgtttg agcggctgcc acaggcgaca ggagctttct gtcttgaaaa caaaaaacaa
      721 ccagaatgag gtggcacagg caaccgccgc gccgcgcgcg ccgccggacg gtacggcaag
      781 ccccagcagc agcaggtttg ccgccagcgc gcccaaggtg gcgagcgcga tcgggcgtgt
      841 ttttcggacg acgttcaaac cgatgccgct gatttctacc agcgtgcaaa acagcggcgg
      901 cagcatacac gatacgacga taaaccggac ggcagcgtag ttttccggca gcaggaggga
      961 ggcgaggggc gagaaaatgc cggtcaggca gagggcggag gcaagcaggg cggcggcgga
     1021 ttctgccgtt gccgagaggc gggcgggcgg ggcgttttct tcgattgcgc ggaaaatata
     1081 cggtgtccag accgttgaaa agatgctttg gaacaataat gccgccccgc cgaacgaaat
     1141 acccatcgaa taaacgccga gctgttccag accggcatat tttttcagga acaaacggtc
     1201 ggcggatgcc agcccccaat aggcgatgct gctcagtgcg atcggtatgc cgtagcgcag
     1261 cccccggtgc aggacggcgg gcgaaaacgg tgcgtgccgg acggccttca gacggcatcg
     1321 gttttgaaac agcaaaaagg cggcggcggc aaggtttgcc agcgcgtaaa cggcggtcag
     1381 gacggcggtg ttcgccggaa agtgcagcag cccgaccgtc agcggcagca gcagcaggat
     1441 ggcgagcttg ggcacgagtt gcgcggacga aaaggcaagg gcgcgccctt ccatacgcaa
     1501 aaccagtaag agaaagcgga tgggcaggaa gctcagttca aacagcacca gcccgatgcc
     1561 ggcggcggca tcgtcgagcg aaaacaggat ttcagacggc agggacgggc gggaaagcag
     1621 cagggcggct atcgcggcgg cagacagcag cggcggcagg aacagggttt tgaacaaggt
     1681 gtctttgtcg gcggcggcat agtattcgcg gacgtatgcc tgatccagcc cgaggcacaa
     1741 taccgacacc gtcagtcccg ccgccgtctg catcagcacg atgcgcccga tgtcgtcggc
     1801 ggggaaatac cacgacagca gcggcaggat gatgacggct aaaaccgcgc tgccgatcga
     1861 gcctgccgcg tagccgagga tttcttttgt gtccattttt gatgtccggg cggcggcggg
     1921 atgctgcctg tgccgtctga agcctttctt gatcggaatt tgacggcttt cagtccgttg
     1981 cggctgccgg cggggtgcgg cagtccgggt tgcgcttttc cgggcgggcg gcggtctgaa
     2041 cggggccgtt ttttatcggc gttattatat agtgaaacgg cgcaaaccct ttaaaaggcg
     2101 ttatcgtttt tccggaacac ggttttgatg tcgtgtccga ggatttcgct tgaaacgggt
     2161 gtccacaagg gcggtgcgga aagggcggcg cggttttcgg gcagggaaag caggtctttg
     2221 ccgctgccga ggattttggg cgaacggtac agcacgattt cgtccgccag atcttctgcc
     2281 agaaatgcgg atgtgagttc ggagcctgct tcgaccatga tttctccgaa accttcgtcg
     2341 gcaaggaggc gcatcaggtg gttcaggtcg attttgccgc ctgccgtttc agacggcatc
     2401 aggatgcgga tgtgtgcgtg ttcccgatag gggcggagtt tatcttcatc gcgttccaag
     2461 gtggcgatgt aggttggaga ttgtccgtcg gtcagaaggt ggctgtccaa gggcaggcgc
     2521 aggcggctgt ctaacacgat gcgtgcgggt tggcgcaaag ttggaaaagc gcggacgttg
     2581 agccggggat tgtccgccaa caccgtgccg atgccggtca gcaccgcgca gctttcggca
     2641 cgcaaaacct gtacgtcggc acgcgcgtct tcgccggtaa tccaaaagct gctgtcgtct
     2701 gaaagggcgg ttttgccgtc cagcgaaacg gcgcatttga ggcggacgaa ggggcggcgg
     2761 cgttcgatgc gcgacaggaa gcctcggttg agttcccttg cctgatgttc gagtaaaccg
     2821 cattccgtct tgatgcctgc tgcttcgagc agggcaagcc ctttgcctgc aaccagcggg
     2881 ttggggtcgc gcatggcggc gacgacgcgt gttacaccgg aacggagcag agcttcggcg
     2941 cagggcggtg tgcgtccgta atggctgcac ggttcgaggg taacaaaggc ggtcgcgcct
     3001 tgtgccattt cgcccgcctg acgcagggcg tggacttcgg cgtgcggttc gcccgctttg
     3061 acgtggaagc cttgcccgac aatttggctg ccgtgtgcga taacgcagcc gacgcgcgga
     3121 ttgggcgaag tggaaaaacg tcccaaagcg gcaagtcgga gggcggtttc catcatggat
     3181 atatctgtgt ccgaaaacat agggataccg tatcagtatg ggttggggag aatcaggctt
     3241 tgcggtctgt tttgacggct tgcgccagcc acgaggcaaa gtctgccgga ttgtcgaagc
     3301 gtttgtgcag ggcggcgaaa cggacggcgg cttccgtgtt gtgctcaagc agctctttta
     3361 gcaccatgtc ggcaagtgcg gcagagggga tgtcgcgctg acccgaagtg tagagcctgt
     3421 gtttcgtcag gcggacggtt tcgtcgatct gttcgggtgt cagggcggat tttcgggcgg
     3481 cggcggtcag gtcgttgcgg aggcgttgtg cattaaaggg cgaacgtttt ttgtccggac
     3541 cgatgacggc gggcattttg agttcggcgg tttcgagcgt gccgaagcgt ttgccgcagc
     3601 tggggcagtg gcggcggcgg cgcacggcgt tgcgttcttc catcaggcgc gaatcggcga
     3661 cgcgggtgtc ggggtgggcg caaaacgggc atttcatagc gtttcttcct gatgccgtct
     3721 gaacgtcaaa ccgatacgcc ggcggcgcgg gcgatttcca gaccttcttc ggcactcata
     3781 tagacggggt tttcgggacg gtcgtgtcgg acgatgttgc cttcgcggaa catgaccagt
     3841 tcgtccacgg caagttggga ccatgattcg tcgcgggtca gtggcagggt ggcgataacg
     3901 gcgacgcggt cggagggcgt ggttacttcg gcaaaatcga ccatcacgtc gtcgtcgagc
     3961 aggcgcgcct tgccgaacgg ggcttggcgg acgatgtagt gcagcagtgt gctggcgtgg
     4021 gcaaacaggg caatgccgtc tgaaagcata aagttaaaca gcccgaactt gcggatttcg
     4081 tgcgtcagcc ccgcaatcgc gtcaaacagc gtgtcgtcgt cgggacgggc ggcaaagcgg
     4141 gtgcgcaggc ggttgaggat gtggcagaac gcgcgttcgg aatcggttgt gccgacgggg
     4201 tggaaaaatt cgccctgttc ggggaaaaaa tcaatcaaat gtccgttgtg ggcaaacagc
     4261 cagtagccgc cccacatttc gcgcataaag ggatgggtgt tcgccagcga ggtttgtcct
     4321 tgcgatgctt tgcggatatg tgcgatgacg ttttccgatt tgatttggta ggcacgcacg
     4381 aggtcggcga cgggggaatt tgcgctcggc ttgtcgtcgt ggaacaggcg cacgcctttg
     4441 ccttcgaaaa agccgatgcc gaaaccgtcg gcatgatggt cggtaatgcc gccgcgccgg
     4501 cggaagcctt caaaggaaaa cataatatcg gtcggcgtat tgcagttcat gcccagcagt
     4561 tgacacatag tttgtccaaa tgattcagat ggtcgcaagt attcggatta taccccgaac
     4621 tgaaaatgcc gtctgaaata cggcttgttc cccattattc ccacgaaaac agaaaacaaa
     4681 gacggaaact taagattccg tcattcccgc gcaggcggga atctggaatt tcaatgcctc
     4741 aagaatttat cgaaaaaaac caaaaccc
//
LOCUS       DAWFAD010000136         4724 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWFAD010000136 DAWFAD010000000
VERSION     DAWFAD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.136"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            297..995
                     /locus_tag="WGO49_001701"
     CDS             297..995
                     /locus_tag="WGO49_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0306783.1"
                     /translation="MKSKHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(1052..2056)
                     /locus_tag="WGO49_001702"
     CDS             complement(1052..2056)
                     /locus_tag="WGO49_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ0306784.1"
                     /translation="MQRQTELKNWLQTIYPERDFDLSFAAADADFRRYFRATFSDGSS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            2126..4534
                     /locus_tag="WGO49_001703"
     CDS             2126..4534
                     /locus_tag="WGO49_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ0306785.1"
                     /translation="MARLFSLKPLVLALGFCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPSVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEMLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
BASE COUNT         1141 a         1439 c         1243 g          901 t
ORIGIN      
        1 ttgaaagggg tgtaaccctt ccgaaccagg gcaacgcata gggcggtgct ttatgtgccg
       61 tcctgtgtgt tggaacatca ttagttcccc aacagccgta tccgtctggg gcgacggcgc
      121 gtattgtacg acagaaaccg cttcagacgg catcgggttt gatgccgtcc gaagcggttt
      181 ccccaaacga aaacgtcctt ttccggaggc aaactgtatc aacacgcgga aatgcaataa
      241 aatagccgga ttccgattga tttccaacat ctgtttccaa catcacggag agccgtatga
      301 aatccaaaca cctcgccctc ggcgttgccg ccctgttcgc acttgccgcg tgcgacagca
      361 aagtccaaac cagcgtcccc gccgacagcg cgcctgccgc ttcggcagcc gccgccccgg
      421 cagggctggt cgaagggcaa aactatactg tccttgccaa cccgattccc caacagcagg
      481 caggcaaagt cgaagtcctt gagtttttcg gctatttctg tccgcactgc gcccacctcg
      541 aacctgtttt aagcaaacac gccaagtctt ttaaagacga tatgtacctg cgtaccgaac
      601 acgtcgtctg gcagaaagaa atgctgacgc tcgcacgcct cgccgccgcc gtcgatatgg
      661 ctgccgccga cagcaaagat gtggcgaaca gccatatttt cgatgcgatg gtcaaccaaa
      721 aaatcaagct gcaagagccg gaagtcctca aaaaatggct gggcgaacaa accgcctttg
      781 acggcaaaaa agtccttgcc gcttacgaat ctcccgaaag ccaggcgcgc gccgacaaaa
      841 tgcaggagct gaccgaaacc tttcaaatcg acggtacgcc cacggttatc gtcggcggca
      901 aatataaagt cgaatttgcc gactgggagt ccggtatgaa caccatcgac cttttggcgg
      961 acaaagtacg cgaagaacaa aaagccgcgc agtaagcccg tttgaaaaat gccgtctgaa
     1021 acttggtttt cagacggcat tttgattggg tttaaaacgt aaagcccgtt tccagttctt
     1081 catcgccgac cagttcgacc aagagcgcgt agagcggggc gagttcggca taacggcgcg
     1141 atacgcggcg caggtaattg aggaaacgcg ggatttcggg acggtatttg tctttgccgt
     1201 cacggtagta caggcgtgcg aagatgcctg caaccttcaa gtgccgctgc acgcccatcc
     1261 attcgaacca gcggtaaaac tcgtcaaacg cttcggggac gggcaagccg gcagcccgcg
     1321 ccttttccca gtagcggata accaagtcca agacaaattc ttcttcccat tcgataaagg
     1381 catcgcgcaa cagcgacacc aaatcgtagg aaatcgggcc gtaaagcgcg tcttggaagt
     1441 ctaaaacgcc cggtctgccg cgcgtcagca tcaggttgcg gacgataaag tcgcggtgca
     1501 catagacttt gggctgcgcc aacaggggcg gcagcagcgt atcgacggtt tgctgccaaa
     1561 gttggcgttg tttgaatgtt aattcgcgcc ccaattcttt tgcgacaaac cattccggga
     1621 acaggttgat ttcgcgcagc atcgtttcac ggtcatattc gggcaaaacc ccttcacggc
     1681 ttgccttctg caattcgacc agctcgccga ttgcctccaa aagcagggct ttgtgcgccg
     1741 cttcgccctg ctcctgaagc atcgcggtca aaaacgtcgt attgcccaag tcgttcaata
     1801 ccacaaaccc caaatccgtg tccgcgtgca atacctgcgg tacattgacc atgtcaaaca
     1861 gtttctgcac tttcaaataa ggtgcgacac tcatcttgtc gggcggtgca tccatgcaga
     1921 cgacactgct gccgtctgaa aacgttgcac ggaaatagcg gcggaaatca gcatccgccg
     1981 ccgcgaagga cagatcgaag tcccgttcgg gatagatggt ctgaagccaa tttttcagtt
     2041 cggtttgccg ttgcataaca gtactaaagc atttcaggtt acaataaacg ctattctaac
     2101 tggcaaaccg acttgagggg cgattttggc tcgtttattt tcactcaaac cactggtgct
     2161 ggcattgggc ttctgtttcg gcacgcattg cgccgccgcc gatgccgttg cggcggagga
     2221 aacggacaat ccgaccgccg gaggaagcgt tcggagcgtg tccgaaccca tacagcctac
     2281 cagcctgagc ctcggttcga cctgcctgtt ttgcagtaac gaaagcggca gccccgagag
     2341 aaccgaagcc gccgtccgag gcagcggcga agcatccatc cccgaagact atacgcgcat
     2401 tgttgccgac aaggtggaag gacagtcgca ggtgcaggtg cgtgccgaag gcaacgtcgt
     2461 cgtcgaacgc aaccggacga ccctcaatac cgattgggcg gattacgacc agtcgggcga
     2521 caccgttacc gcaggcgacc ggttcgccct ccaacaggac ggtacgctga ttcggggcga
     2581 aaccctgacc tacaatctcg agcagcagac cggggaagcg cacaacgtcc gtatggaaac
     2641 cgaacacggc ggacggcggc tgcaaagcgt cagccgcacc gccgaaatgt tgggcgaagg
     2701 gcattacaaa ctgacggaaa cccaattcaa cacctgttcc gccggcgatg ccggctggta
     2761 tgtcaaggcc gcttccgtcg aagccgatcg ggaaaaaggc ataggcgttg ccaaacacgc
     2821 cgccttcgtg ttcggcggcg ttcccatttt ctacacccct tgggcggact tcccgcttga
     2881 cggcaaccgc aaaagcggcc tgcttgttcc ctcactgtcc gccggttcgg acggcgtttc
     2941 cctttccgtt ccctattatt tcaaccttgc ccccaatctc gatgccacgt tcgcgcccag
     3001 cgtgatcggc gaacgcggcg cggtctttga cgggcaggta cgctacctgc ggccggatta
     3061 tgccggccag tccgacctga cctggctgcc gcacgacaag aaaagcggca ggaataaccg
     3121 ctatcaggcg aaatggcagc atcggcacga catttccgac acgcttcagg cgggtgtcga
     3181 tttcaaccaa gtctccgaca gcggctacta ccgcgacttt tacggcaaca aagaaatcgc
     3241 cggcaacgtc aacctcaacc gccgtgtatg gctggattat ggcggcaggg cggcgggcgg
     3301 cagcctgaat gccggccttt cggttctgaa ataccagacg ctggcaaacc aaagcggcta
     3361 taaagacaaa ccgtatgccc tgatgccgcg cctttccgcc gattggcgca aaaacaccgg
     3421 cagggcgcaa atcggcgtgt ccgcacaatt tacccgcttc agccacgaca gccgccaaga
     3481 cggcagccgc ctcgtcgtct atcccgacat caaatgggat ttcagcaaca gctggggtta
     3541 cgtccgtccc aaactcggac tgcacgccac ctattacagc ctcaaccgct tcggcagcca
     3601 agaagcccga cgcgtcagcc gcactctgcc cattgtcaac atcgacagcg gcgcaacttt
     3661 tgagcggaat acgcggatgt tcggcggcgg agtcctgcaa accctcgagc cgcgcctgtt
     3721 ctacaactat attcctgcca aatcccaaaa cgacctgccc aatttcgatt cgtcggaaag
     3781 cagcttcggc tacgggcagc tctttcgcga aaacctctat tacggcaacg acaggattaa
     3841 caccgcaaac agcctttccg ccgccgtgca aagccgtatt ttggacggcg cgacggggga
     3901 agagcgtttc cgcgccggca tcggtcagaa attctatttc aaggatgatg cggtgatgct
     3961 tgacggcagc gtcggcaaaa aaccgcgcaa ccgttccgac tgggtggcat ttgcctccgg
     4021 cagcatcggc agccgcttca tcctcgacag cagcatccac tacaaccaaa acgacaaacg
     4081 cgccgagaac tacgccgtcg gtgcaagcta ccgtcccgca cagggcaaag tgctgaacgc
     4141 ccgctacaaa tacgggcgca acgaaaaaat ctacctgaag tccgacggtt cctattttta
     4201 cgacaaactc agccagctcg acctgtccgc acaatggccg ctgacgcgca acctgtcggc
     4261 cgtcgtccgt tacaactacg gttttgaagc caaaaaaccg atagaaatgc tggcgggtgc
     4321 ggaatacaaa agcagttgcg gctgctgggg cgcgggcgtg tacgcccaac gctacgttac
     4381 cggcgaaaac acctacaaaa acgctgtctt tttctcactt cagttgaaag acctcagcag
     4441 tgtcggcaga aaccccgcag acaggatgga tgtcgccgtt cccggctata tccccgccca
     4501 ctctctttcc gccggacgca acaaacggcc ctgaccgtcg gaaacccggc aggagcaccg
     4561 ttcccgcaca agacggcatt ccaccgacaa ccccaaaccc gccatcaaag gcaggattca
     4621 aacgataagg aaagaatgat gaaaatcaaa gccctgatga ttgccgccgc attgctggca
     4681 gcagccgatg tccacgccgc accgcaaaag gcaaaaaccg catc
//
LOCUS       DAWFAD010000137         4714 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWFAD010000137 DAWFAD010000000
VERSION     DAWFAD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.137"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(37..246)
                     /locus_tag="WGO49_001704"
     CDS             complement(37..246)
                     /locus_tag="WGO49_001704"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306786.1"
                     /translation="MPSENLSDGIFLPKQPYFLSGLQNLSETTETTLPSFPQKRESRT
                     QHGKNLSEATTIFSSSFPQKRESRT"
     gene            complement(264..1202)
                     /locus_tag="WGO49_001705"
     CDS             complement(264..1202)
                     /locus_tag="WGO49_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0306787.1"
                     /translation="MLELNGLCKRFGNKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIWLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAERLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1211..2085)
                     /locus_tag="WGO49_001706"
                     /pseudo
     CDS             complement(1211..2085)
                     /locus_tag="WGO49_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
     gene            complement(2267..2343)
                     /locus_tag="WGO49_001707"
     tRNA            complement(2267..2343)
                     /locus_tag="WGO49_001707"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2307..2309),aa:Met,seq:cat)
     regulatory      2493..2592
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2683..4584
                     /gene="thiC"
                     /locus_tag="WGO49_001708"
     CDS             2683..4584
                     /gene="thiC"
                     /locus_tag="WGO49_001708"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ0306788.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGAEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLNE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKSGGNVTQLHYARRGIITPE
                     MEFVAIRERLKLDELSQKSEYAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTADRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTSKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1145 a         1330 c         1243 g          996 t
ORIGIN      
        1 acggaaactt atcgggaaaa acggtttctt tagattttat gttctagatt cccgcttttg
       61 cgggaatgac gatgaaaaga ttgttgtcgc ttcggataaa tttttgccgt gttgggttct
      121 agattcccgc ttttgcggga atgacggcag ggtggtttct gttgtttcgg ataaattttg
      181 cagccctgat aaaaaatatg gctgctttgg taaaaaaatg ccgtctgaaa ggttttcaga
      241 cggcattttg tttttaagaa gcatcaacgg aagcggacga tttccctgtc ttcgatatgg
      301 atgcgtaccg tatcgtttcc cgatatttca ccggcgtgcg gcatatcgag gttcagccac
      361 aggatgccgt gttccggatg gaggacggac aggctgaacg attcgggcaa acaggtacgg
      421 gataatacgc ggcactccat gccgtcttgg tcgaaacgca ccgcatgttg cggaatatgg
      481 cggtcatcgt cggtattggg caaacccatc agtcgggcga cctgcacgcc ggcaggcgtt
      541 tgaaccaagg tttcgggcgt accgcattga aggattttcc cctcgtgcat gacggcgatt
      601 tcgtccgccg ccgtgcaggc ctcttcgggc gaatgcgtca ccaaaacggc agggatgccg
      661 cccttgcgga tacgttcggc agtcatgcgg cgcagccggt cgcgcaaatg cgtgtccaaa
      721 ctggaaaacg attcgtccaa cagcagcagg gaagggcgga caaccaaagc gcgcgccaac
      781 gccaaccgtt gcttctcgcc tccggaaagt ttttcaggct tgcggtgcgc ctcgttttcc
      841 agtccgactt cggcaagtgc cgccatggca aggcgttcgg cttcggcttt cggcattttt
      901 tgcattttca aaccgaatgc cgcattttcc agcgcactca tatggggaaa cagcgcgtaa
      961 tcttgaaaca tcagagaaat acggcgtttt tcgggcggca tacgggtaat gttttccccg
     1021 ttcagccata tttccccacc gtccggccgg acaatccccg caatcatatt cagcagggtg
     1081 gattttccgc atcccgaccg ccccaaaacg gcgagtattt tgccgcgccc gacagtcagg
     1141 cagatgttgt cggcgacggt tttattgccg aagcgtttgc agagtccgtt cagttcaagc
     1201 atggcgcatc ctataaacgt atgccgtcca gccactcgga cagcggatgg gcggcatcaa
     1261 tcagaccgta acggcaaaac gcgtccagcg taaccagttg cgcgtcgtgc atcatgtttc
     1321 ccgacaacat ggtggcaacc aagctgccga tgtccatttt ctcaaaacac gcaacctcac
     1381 cgtcctgatt ttcaggtagg aaggtttcgg gcaggacggc atcgaataca tacaggattt
     1441 cattgtgcac accccagctg acggggcgca ggctgtgcag ctgcgatacc gggcggatga
     1501 gcggaaacag cgttttatcc aaaccggctt cttcgctgct ttcgcgacac acggtttcag
     1561 acggcaattc accgctggaa acaccgccgg cggcagtatt gtcgagtttg tcgggatcga
     1621 ctgctttgtg cggactgcgc ctgcctatcc agaaatgcca tcggccgtcc gattcggtca
     1681 gaccgttgag atggacggcg cggctgagta gtccgaacgg gcggaaagcg gcgcgttcaa
     1741 gtgagaacaa gatattgccg ccgccgtcgg tcggtcgaaa cactcgttgc gccatccgtg
     1801 aagcagcccc gccttgttcc atgtgcgggt gaggtgctgc aagcggccgc ccatatctga
     1861 ccagccgtcc gcattcagaa aaatgccgtc tgaagactcc gagcagcctg cctcccagtc
     1921 ttttttgacg cgctccgccc attccggcga caggttgccc aaaggcaggc cgttcagata
     1981 cagtgttttc cagcaacttt ctgcaccgta acttgctttt gcccactcga acagggcatc
     2041 aaggtcgtgt ttgctgacgg attcggtaaa acggacggtc ggcatagtcg gctttcttga
     2101 agacaatgcg gaagattgta gccaaactgc ctgaatttgc aaccgcaaac agacggcggg
     2161 ctgccgttcc gatattgcgg caggaaggaa aatccggcaa aaaacggagg cggcaggaaa
     2221 caggcaaaac aaaacccctc ggcattgaac cgaggggttt gatatttggt tgcgggagca
     2281 ggattcgaac ctacgacctt cgggttatga gcccgacgag ctaccatgct gctccatccc
     2341 gcgtcagaag atgaaactat acggcagatt ttttatgttg tcaaacatta tttccgcgaa
     2401 aaatcataaa attttgccgt ccggcggttt atgcctgatt tgaaatatca ttttctttac
     2461 aaaagttcat gttcgtgatt taattttggt taacatcgaa acaggggtgc tgcctgatgt
     2521 tcaggcggct gagaaatacc ctttacaccc gatcgggata atacctgcgt ggggagtttt
     2581 cacggattct gtctttcaga cagcattggt tttcaaatgc cgtctgaaaa cgcaaaacgc
     2641 tcctgtttct ttattctaaa cgagaaaaca ggagcatttt ttatgactac gccaaaaaaa
     2701 accgccaaaa cttccggcaa cgaagcgcgc gaacttgccg acttgagcga agacatcggt
     2761 atccgcttca aatatccgaa ctcggaacgc gtgtatctgc aaggcagccg cgacgacatc
     2821 cgcgtgcctt tgcgtgaaat ccgtcaggac gacacctata cggcgcaagg cgcggaagcc
     2881 aacccgccga ttcccgtcta tgacaccagc ggcgtgtacg gcgatccagc ggcacacatc
     2941 gacctgaaac aaggcctgcc gcacatccgc accgcatggc tgaacgaacg cggcgatacc
     3001 gaaatcctgc ccaagctctc cagcgaatac ggcatcgaac gcgcacacga tccgaaaacc
     3061 gcccatctgc gcttcaacca aatcacccgc ccgcgccgcg cgaaaagcgg cggcaacgtt
     3121 acccagcttc actacgcgcg ccgcggcatc atcacgcccg aaatggagtt tgtcgccata
     3181 cgcgaacgtt taaaattaga cgaattgtcc caaaaatcgg aatacgccaa actcttgaaa
     3241 cagcacgcag ggcaaagttt cggcgcgaat atcccgaccc atcccgacca aatcacgcct
     3301 gaattcgtgc gccgagaaat tgccgccgga cgcgcgatta tccccgccaa catcaaccac
     3361 cccgaactcg aaccgatgat tatcggccgc aacttccgcg tcaaaatcaa cggcaacttg
     3421 ggcaattccg ccgtcacttc cagcctgacc gaagaagtcg aaaaaatggt gtggtcgctg
     3481 cgttggggcg cggacacgat tatggatttg tccaccggcg cgcacatcca tgaaacgcgc
     3541 gaatggatta tccgcaacgc gcccgtcccc atcggcaccg tgccgattta ccaagcgttg
     3601 gaaaaaaccg gcggcattgc cgaagatttg acttgggatt tgttccgcga caccttaatc
     3661 gagcaggcgg aacaaggcgt ggactatttc accatacacg caggcgtgtt gctgcgttat
     3721 gtgccgatga ctgccgaccg cctcaccggc atcgtatcgc gcggcggttc gattatggcg
     3781 aaatggtgtt tggcacatca tcgggaaaac ttcctttaca cgcatttcga cgaaatctgc
     3841 gaaattatga aggcgtatga cgtatcgttc agcctcggcg acggtttgcg tcccggctgc
     3901 attgccgatg ccaacgacga atcccaattc gccgagctgc acaccttggg cgaattgacc
     3961 tccaaagcgt ggaaacacga cgtacaggtg atgatcgaag gtcccggcca tgtgccgctg
     4021 caacgcgtca aagaaaatat gaccgaagag ttgcaacact gttttgaagc accgttttac
     4081 accctcggcc cgctcgttac cgacatcgcc cccggctacg accacatcac ctcgggcata
     4141 ggcgctgcca atatcggctg gtacggcacg gcgatgctct gttacgttac ccccaaagaa
     4201 catttgggac tgcccgacaa agaagacgtg cgtaccggca tcatcaccta caaactcgcc
     4261 gcccacgccg ccgacctcgc caaaggctgg ccgggcgcac agttgcgcga caacgccctg
     4321 agcaaggcgc gtttcgagtt ccgctggcgc gaccagttcc gcctaagcct cgaccccgaa
     4381 cgcgccgaaa gcttccacga cgaaaccctg cccgccgaag gcgcgaaaat cgcccacttc
     4441 tgttcgatgt gcggccccaa attctgctcg atgaaaatca cgcaggaagt gcgcgactac
     4501 gccgacaagc aaaaagccca acggcagggc atggaggaaa aagcggtcga gttcgtcaaa
     4561 aaaggagcga agatttacag ctaaaacgtc aagcaaaaaa tgccgtctga aacccggaaa
     4621 aaaggcttca gacggtattc tttcgcttgt gaaaatatag tggattaaca aaaatcagga
     4681 caaggcgacg aagccgcaga cagtacagat agta
//
LOCUS       DAWFAD010000138         4650 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWFAD010000138 DAWFAD010000000
VERSION     DAWFAD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.138"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            110..1051
                     /gene="miaA"
                     /locus_tag="WGO49_001709"
     CDS             110..1051
                     /gene="miaA"
                     /locus_tag="WGO49_001709"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ0306789.1"
                     /translation="MPTPKAFALLGPTACGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSHAERSFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKYPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSPTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRLYPSLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVTDPFSDGNSCTRLIEAAKRFFGA"
     gene            1055..1774
                     /locus_tag="WGO49_001710"
     CDS             1055..1774
                     /locus_tag="WGO49_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family DNA transformation protein"
                     /protein_id="HEZ0306790.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGAVQTFLLLKASGLT
                     LTKSTLWQLQSLLDGTPPQEMAQHDKTRLLAELKNHPPVAAFPPQGEMAHFMGLALEQ
                     AKLSALMGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            1779..2267
                     /locus_tag="WGO49_001711"
     CDS             1779..2267
                     /locus_tag="WGO49_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ0306791.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGIWGTKYTGAGKLAVRGALVGSIIGMFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLVVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            2333..3085
                     /gene="rlmB"
                     /locus_tag="WGO49_001712"
     CDS             2333..3085
                     /gene="rlmB"
                     /locus_tag="WGO49_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ0306792.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(3147..4538)
                     /locus_tag="WGO49_001713"
     CDS             complement(3147..4538)
                     /locus_tag="WGO49_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ0306793.1"
                     /translation="MSGQLGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLIFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
BASE COUNT         1162 a         1490 c         1126 g          872 t
ORIGIN      
        1 aaggcatacg gtaaacacgc ccccttgccg tcggcgtgct tccgcaaacc aaaatataac
       61 gtcggcacgc tttccgctat aatccgtatt tttccaaacc tgaaacgtta tgccgacccc
      121 gaaagccttt gccctgctcg ggccgaccgc ctgcggcaaa accgcgctcg ccctcaaaat
      181 tgccgaaacc ttgcctgtcg aaatcatcag cctcgattcc gcgctggttt accgcgatat
      241 ggacatcggc acggcgaaac cgtctcatgc cgagcgttcc tttgtaccgc accaccttat
      301 cgacatcatt acgcctgttc agacatacag tgccgcccgt tttgtcgaag actgcacgcg
      361 tctgattggc gaaatcacgg cgcgtgggaa atatccgctg attgtcggcg gcacgatgat
      421 gtatttccgc gccctgaccc aaggtttgaa cgatttgccc gaagccgacg cctgcctgcg
      481 tgccgacttg gacgaacaaa aacaaatgta cggcttggat ttcctctacc gcaccctgca
      541 aaaagtcgac cctgaaacag catgccgtct gaaaccgaac gacagccagc gtatcggacg
      601 cgctttggaa gtttattatt taaccggcaa accgatgagt acgcatctcg gcagcccaac
      661 ctcccatacg ctccccttcg atttgcatac cgccgccctg attcccgaaa accgcgcccg
      721 cctgcatgaa aacatcgccc tgcgtttcca cctgatgctt gaacagggct ttatcggcga
      781 agtagaaaac ctgcgccgcc tctatcccag cctgaccgcc gactcccctg ccatacgctg
      841 cgtcggctac cgtcaggcat gggaatatct ggacggaaaa accgatttcc cggcatttgt
      901 cgagaaaggc attgccgcca cgcgccaact tgccaaacgc caactgactt ggttgcgcaa
      961 aacaccttta gactgcgtta ccgacccgtt ttcagacggc aattcatgca cacgcctgat
     1021 tgaggcggca aaacggtttt tcggcgcata aactatgctg accacgccgc cgctcgcccc
     1081 caaaaccgtc gccgccctac acaggctcgg tatccgtacg ctcgaagaac tgcggcaaaa
     1141 cggcgcggtt caaaccttct tgttattgaa ggcatcaggt ttgaccctca cgaaaagcac
     1201 gctgtggcag cttcagtcgc tgctggacgg cacgccgccg caagaaatgg cgcaacacga
     1261 taaaacccgc ctgcttgccg agttgaaaaa ccatccgccc gtcgccgcct ttcccccgca
     1321 aggcgaaatg gcgcatttca tgggtttggc attggaacag gcgaaactgt ccgcattaat
     1381 gggcgaaata cccgtcgggg cagtcatcgt ttcagacggc aaaatcatcg catcggcaca
     1441 caacacctgc attgccgact gcaatgtcag ccggcacgcc gaaatcaacg ccttggcaca
     1501 ggcaggcagc gaaatgcaaa actaccgcct tgacggatgc gacatctaca tcaccctcga
     1561 accctgcgcc atgtgcgcgt ccgcactgat acaggcacga atcaggcgcg tgatctacgg
     1621 tgcggcagaa cccaaaaccg gcgcggcagg aagcatcgtc aacctgtttg ccgacaaacg
     1681 cctcaatacg cacaccgcca tacggggcgg aatcctgcaa gaagaatgcc gcgccgtatt
     1741 aagccgtttt ttccaaaaca aaagaaaagg ttgaacctat gaccgccttg ctcgtcatcc
     1801 tcgccctcgc cctgatagcc gccggtacgg cgggcatcgt ttaccccgcc ctgcccggat
     1861 tggcattgat gtttgccgga acctggctgc tcgcctactc cggcggctac caaatctacg
     1921 gcgcgggcgt tttgtggacg gtcggactca tcagccttgc cggcatactg gcggactatg
     1981 tggcaggcat atgggggaca aaatataccg gagcgggcaa gctcgccgtt cgcggcgcat
     2041 tggtcggcag tatcatcggt atgtttttct cccttcccgg actaatactc ggtcccttta
     2101 tcggcgcggc ggcaggcgaa ctgatcgatc ggcgcaatat gcttcaggca ggtaaagcgg
     2161 gcttgggtac gctgttgggg cttgtcgtcg gcacggcgtt caaaatcggc tgcgccgtat
     2221 ccatcttgtt tatcctgttg gtgaaataca tcgcctacct gttttaaccc cttcagacgg
     2281 cataccggca aattgctaca atgccgtctg aacccttttc acaaagcacc atatggcaaa
     2341 ccaaagacct atctacggct tccatgccgt caacgcccga ttgtggcaaa accccaaatc
     2401 catcgtcgaa ctctatgtcc aagaaggcaa aaacgatgta cgcacgcgcg aagtgttgga
     2461 aaaggcggca aacgaaaaca tccgcgtata ttttgccgat gccgaccgcc tcaacgccat
     2521 cagcaaaggt gcgcgccatc agggcgtggt cggatttatc gatgcctcca aaaaccacgt
     2581 ccacctcgaa gacgtattgg aaaacttaag cgaaccgccg ctgctgctga tactcgacgg
     2641 catcaccgat ccgcacaacc tcggcgcgtg cctgcgtacc gccgacgcaa tgggcgtaca
     2701 tgccgtcatc gcaccgaaag acaaaagcgc ggggctgaac gccaccgtca gcaaagtcgc
     2761 cagcggcgcg gcggaaaccg tcccctacat caccgtaacc aacctcgccc gcaccctgcg
     2821 cgagctgaaa gaatacggca tttggatcat cggcaccgat atgggcggca acgccgacct
     2881 ttaccattgc gatctgcccg acagcgcggc atgggtgatg ggcaacgaag gcgacggtat
     2941 gcgccgcctg acacgcgaac attgcgacat gctggtgtcc atacccatgt tcggcacggt
     3001 cgaaagcatg aacgtctccg tgtctgccgg aatggtatta agcgaaaccc gccggcagcg
     3061 cgtattaaaa aacgaaaaag cgtaagcctc aaaaacaatg ccgtctgaaa cagggattct
     3121 gtttcagacg gcattcgttt tcaaaatcag tgttccaagt ggtcggtatc aaacttgacc
     3181 gccgcctcgg ttttatcttc gggcaagacc aaattcagca agactgccgt aatgccgccg
     3241 gcggaaatag agttttggaa caagacaggc aggtttttaa acacttccgg ctcaaacgcg
     3301 acacccaagc ccaaaccgac cgacgttgcc gcaattaccg cttcgcgcct gcggatgccg
     3361 tggctgacca aaatccgcac gcccgcaatc gcaatcaagc cgaacatcaa aaccatcgcg
     3421 ccgcccaaca ccggactcgg aatcgtcgta aacgcgcgtc cgacaacggg gaacagaccc
     3481 aacagcacca aaatcacggc aatatatttg cccacatggc gcgaagccac gccggtcatc
     3541 tgaatcacgc cgttgttttg cgcaaacgtc gtcagcggca gcgaacccaa agccgtcgca
     3601 atcaccgaca ccaagccgtc agccaacacg ccgccgcgca ggcgtttggt gtattcctcg
     3661 ccttcaatcg gctgatcgga caccattgcc gtcgccgtca aatcgccgac cgcctcaaac
     3721 acgctcaaca ggaaaatcgc gcccgccaca ataaatgcgt gccagtcgaa agcaaaaccg
     3781 tatttaaacg gtacgggcag cgtaaccagc ggcaggtttt gcagcgcgga aaaatccacc
     3841 ttgcccaaaa acagcgcgac aatgtaaccg gcaatcagac cgaccgcgat gccgctcatc
     3901 cgcaacagcg ggtttttcat gcagttgaat atcaacacaa tcagcaacac cagcgaagcc
     3961 agccccaagt tttccatcga gccgaacgtg ccgtccgcct tcgcgccgaa gccgccgccg
     4021 aaatcggtaa taccgacgtg taccaaactc aagccaatga gcatcacgac cacgccgctg
     4081 accgtcggcg taatcacttt tttcaaatac ggcaaaagcc acgccgagaa acacaccaaa
     4141 aacgcgccga caaacgatac acccaagagc gtcgaaatca tcgcatcctt agtcaaaccg
     4201 ccctccttca tccctgcgcc cagcgcaatc atcacggtaa cgaacgaaaa attcaccgac
     4261 tggatggaca gcatccccga accgaccggc ccgaagcggt tgacctgcaa ataagtgccg
     4321 acacccgacg caaccatcgc catcgacacg agatacgccg tcatctccac cggcaattcc
     4381 agcgcgccgc ccacaatcag cgcgggcgta atcatcggca caaaaatcgc caaaagatgg
     4441 gtaaccgcgc tcaagagcgc attaccgaac ggcggcctgt cttccaaacc gtacaccaaa
     4501 tcaggcgcat ccgcaccttt gcccaactga ccgctcattg tatttcctta ttttaaaggt
     4561 tgaaaaatcc aagcgcggat tgtagcgaat ctgccgcacg gcttcaattt gcatttcaga
     4621 cggcatccgt ctggtccaca cgtttaccac
//
LOCUS       DAWFAD010000139         4574 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWFAD010000139 DAWFAD010000000
VERSION     DAWFAD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.139"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(58..1851)
                     /locus_tag="WGO49_001714"
     CDS             complement(58..1851)
                     /locus_tag="WGO49_001714"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ0306794.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAESQGRPLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIVKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            2096..3205
                     /gene="proB"
                     /locus_tag="WGO49_001715"
     CDS             2096..3205
                     /gene="proB"
                     /locus_tag="WGO49_001715"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ0306795.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITCQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAAEHQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSNATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            3218..4480
                     /locus_tag="WGO49_001716"
     CDS             3218..4480
                     /locus_tag="WGO49_001716"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ0306796.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRLDLEAAAGNIPESMTDRLLLDEKRIGAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGRESSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRNRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVW
                     HIETHSTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1045 a         1244 c         1271 g         1014 t
ORIGIN      
        1 gccgcaatgc cgtctgaagc ctttttatgc ttcagacggc atcgtctgtt ttcacgctca
       61 aatcgtaccg ctgccttgcg ctttcggttt ggcggcacta aattccagtt tgctcaaagc
      121 ggaaaggtag gctttggcag tggcaaccaa aacgtcggta tccgcgccct gaccgttgac
      181 gacgcggttg ccgcgtgcca gacggacgct ggtttcgcct tggctttccg taccttgcgt
      241 gacggcgttg acggaataaa tctgcaaagt cgcgccgctt tgcgccacgc tttcaatcgc
      301 tttgaaaatt gcatcaaccg gaccggaacc ggttgcggaa gcgcgttttt cttcgccttt
      361 gatgctgaac acgatgtcgg cgcgcggctc ttcgccggtt tcggtgctga ttttttggga
      421 gatgaatttg tagctctcgg cattcatgct gcccatttcg tcggatacca gtgcgtgcag
      481 gtcttcatcg aagatttcgc gttttttgtc ggcgagttct ttgaagcggg caaacgcggc
      541 gttcagtgcc tcttcgcttt ccaactcgat gcccaaatcc gccagcttgg ttttgaaggc
      601 gttgcggccg gacaatttgc ccaagctcaa acggtttgtt gcccagccga ccgattcggc
      661 ggacataatc tcgtaagttt cgcggtgttt cagcaccccg tcctgatgga tgcccgattc
      721 atgcgaaaag gcattggcac cgacaatggc tttgttgggc tgcacgggat agcccgtaat
      781 ggtggacacc agtttggacg aaggcacgat ttgcgtggta tcgatgccgg tttccaagcc
      841 gaacaagtcg tggcgcactt tcaacgccat cacgatttct tcaaccgaag cattgcctgc
      901 acgttcgccc aagccgttga cagtacattc cacctgacgc gcaccgcctt ttaatgcggc
      961 aagcgaattg gcaaccgcca agcccaaatc gttgtggcag tgtgccgacc aaacgacttt
     1021 gccgccgttg ggcgttttga caatcagttc gcggaaaaat tcttcggttt tgtacgggat
     1081 ggaatagccg acggtatcgg gaatattgat ggtggtcgcg cccgcttcaa ttaccgcgcc
     1141 gcagatttcg gcaaggaaat cgatttccga acgcaacgcg tcttcgcagg aaaattccac
     1201 atcgtcggtg tattcacgag cgattttcac cgctttgacc gccgcctcaa tcacctgctt
     1261 cggcttcatc ttcaatttgt actccatatg gatggggctg gtggcgatga aggtgtggat
     1321 gcgttttttc ggcgcgggcg caacggcctc acccgcctga cggatgtccc gctcgatggc
     1381 gcgggacaat gaacagaccg ttgatttggt aatggttttc gcaatcgcat tgaccgcctc
     1441 gaaatcgccc gggctggcag cggcaaaacc cgcttcgatg atgtccacac ccaatttttc
     1501 cagctggcgg gcgacgcgga ttttttcctc tttggtcata gcggcgccgg gcgactgctc
     1561 gccgtcgcgc agggtggtat caaaaataat tacgcggttg gtctgtgtca tttctgttct
     1621 ctccagagat tcgttttttt gtaaaaaagc attcaaatcc agcggtctgc cttgcgattc
     1681 ggcaagcgcg gtcagctttt gcagttgcgc cagaggcagc gagccgcgcg tgcgccattt
     1741 atagatggcg gcggtcgtcg cggcattttc gggatcgtgc tgtttcaacg cttcggcaag
     1801 cgcgttcacg ccgccgaaat aagcaaccaa gcggtctata tctaattgca tggtagtcct
     1861 tgtctaaaat aaatcagcct acagcagaaa tatggacagg attatacgca ttttggacaa
     1921 aacggcaata taaatttgaa aaatctcaat aagccgccat agattttatt aattttatac
     1981 attttgtcca ttttattgca gacgcggcgt gattgttgac aattgcggca gaacagggca
     2041 aattcggcgg cacaaggtgg caacgagaca ggacaaacca acaaggatgg aaacaatgaa
     2101 atacaaaaga atcgtattta aagtcggcac atcttcgatt acccattcgg acggcagtct
     2161 ctcgcgcggc aaaatccaaa ccatcacctg ccagcttgcc gcattgcatc atgcgggaca
     2221 cgagctggtc ttggtgtctt ccggcgcggt tgcggcaggg ttcggtgcgc tgggtttcaa
     2281 aaaacgtccg gtcaaaatcg ccgacaaaca ggcttccgcc gccgtcgggc aggggctgct
     2341 gatggaagaa tatacggcaa acctgtcttc agacggcatc gtgtccgcac aaatcctgct
     2401 cagccgcgcc gactttgccg acaaacgccg ctaccaaaat gccggcggcg cactttccgt
     2461 gctgctgcaa cgccgcgccg tccccatcat caatgaaaac gatacggttt cggttgagga
     2521 attgaaaatc ggcgacaacg acacattgag tgcgcaagtg gcggcgatga tacaggcaga
     2581 cctcttggtg ctgctgaccg acatagacgg tctttacacg ggcaacccga acagcaatcc
     2641 cgatgccgta cggctggaca aaatcgaaca catcaaccat gaaatcatcg aaatggcggg
     2701 cggctcgggt tcggcaaacg gcacaggcgg tatgctgacc aaaatcaaag cggcgacgat
     2761 tgcgaccgag tccggcgtac cggtgtatat ctgttcctcg ctcaaacccg atgcacttgc
     2821 cgaagctgcc gaacatcagg cggacggctc gtttttcgtc ccccgtgcca aaggtttgcg
     2881 gacgcagaag caatggctgg cgttctattc cgaaagccgg ggcagcgttt atgtggacga
     2941 aggtgcggaa cacgctttgt ccgaacaggg gaaaagcctg ctgatgtcgg gcattgccgg
     3001 aatcgaaggg catttttccc gtatggacac tgtaaccgtg tacagcaacg caaccaaaca
     3061 gcctttgggc aaagggcgcg tcctgttcgg ttctgccgcc gccgaagacc tgctcaaatc
     3121 gcgtaaggcg aaaggcgtgt tcatccatcg ggacgactgg atttccatca cgcccgaaat
     3181 acgcctgctt ctgaccgaat tttagaaaag gaaacccatg tcaaacacac aaaaacagct
     3241 tgcccttgcc aaagcggcaa aaaaatccgt caacacggcg gatacggaag aaaaaaaccg
     3301 cgcgctgctt gctatggcgg acagcttgga agcggcggcg gcagatattt tggcggcaaa
     3361 ccgtttggat ttggaagcgg cggcaggcaa cattcccgaa agcatgaccg accgcctttt
     3421 gttggacgaa aaacgcattg gcgcgatggc ggacggcatc agggcggttg ccgcgctgcc
     3481 cgaccccgtg ggcgaaatac tggaaacctc gactctgccc aacggcttgg aaatcgtcaa
     3541 aaagcgtgtg gcgatgggcg ttatcggcat tatttacgaa agccgcccga acgttacgtc
     3601 cgatgcggcg gctttggcac tgaaaagcgg cagcgcggtc gtactccgca gcggcaaaga
     3661 tgcattccaa tccgcacgcg ccatcgttgc cgccctgaaa acggggttgg cacaaacccg
     3721 catcgacccc gacgcgctcc agttgattga agacaccggg cgcgaaagca gttacgaaat
     3781 gatgagggcg aaagattatc tagacctgct gattccgcgc ggcggggcgg ggctgatacg
     3841 ggcggtggtt gaaaatgccg tcgtgccggt cattgaaacg ggaacgggca tcgtccacat
     3901 ttatatcgac aaggacgcgg attgggacaa ggcgctccgt atcgtgtaca acgccaaaac
     3961 cagccgcccg tccgtgtgca actcgatgga agtgctgctg gtgcatgaag acattgctgc
     4021 cgacttcctg cccaagctcg aacggctgtt ggttcgcaac cgtatagaag ccggactgcc
     4081 gcccgtccgc ttccgtttgg atccgcaggc ggcgcggcat atcggcggcg aagcggcggg
     4141 tgcagacgat ttcgataccg agtttttaga ctacatcctc gctgtgaaaa ccgtcgcttc
     4201 ggtcgaagag gcggtctggc acatcgaaac ccacagcaca caccattcag acggcatcgt
     4261 taccgaaaac cgccacgctg cggactattt cacgacccat atcgattctg ccgccgtgta
     4321 tgtcaacgcg tccacgcgct ttaccgacgg cggcgaattc ggcttgggtt gcgaaatggg
     4381 catctccacg caaaaactcc acgcccgggg tccgatgggt ttgaaagagc tgacgagtta
     4441 caaatacatc gtacaaggca cgggacaggt cagggaataa tccgaattca aatgtctgat
     4501 atagtggatt aactttaaac cggtacggcg ttgcctcgcc ttagctcaaa gagaacgatt
     4561 ctctaaggtg ctga
//
LOCUS       DAWFAD010000140         4567 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWFAD010000140 DAWFAD010000000
VERSION     DAWFAD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.140"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..988
                     /locus_tag="WGO49_001717"
     CDS             <1..988
                     /locus_tag="WGO49_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ0306797.1"
                     /translation="DFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFSVLADGVRE
                     NLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGRNALHGGSH
                     GLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDNRLTVSYRATALG
                     DTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVFDFSRPKPL
                     DAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGLVIFTAAPQ
                     DFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIESLS"
     gene            1222..3480
                     /locus_tag="WGO49_001718"
     CDS             1222..3480
                     /locus_tag="WGO49_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ0306798.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFDVCKFLSVAQKELALIRWEAVSIDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDDRSYIATQTVTNPFGVEQFIVNAEQ
                     TFAGSFKALGGSQTDWQVSNSFEAEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGSDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRNGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVRAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTAALEK"
     gene            3493..4158
                     /gene="pgmB"
                     /locus_tag="WGO49_001719"
     CDS             3493..4158
                     /gene="pgmB"
                     /locus_tag="WGO49_001719"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ0306799.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGISIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVSEAEFAELTRRKNDNYVEMIQAVKPEDVYPGILPLLET
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKASGALPIGVGKAEDLGSDIPLVSGTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(4368..>4567)
                     /locus_tag="WGO49_001720"
     CDS             complement(4368..>4567)
                     /locus_tag="WGO49_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0306800.1"
                     /translation="QTKRNRYLSKTRYVVEQSFGTLHRKFRYARSTYFGLIKVSAQSH
                     LKAMCLNLLKAANRLSAPAAA"
BASE COUNT         1074 a         1377 c         1209 g          907 t
ORIGIN      
        1 cgatttcggc ctgatcgacg ggcgtgccgt aaccggctat gtgctgtcca atcggcgtgg
       61 tacgcgtgtc tgcgtgctgg acttgggcgg gattgtgcag gaattttccg ttttggcaga
      121 cggcgtgcgc gaaaacctcg tggtgtcgtt cgacgatgcg gcttcctatg cggacaatcc
      181 gtttcagatt aacaagcaga tagggcgcgt ggccggacgc atccgcggtg cggcgttcga
      241 catcaacggc aggacttacc gcgtggaggc caacgaaggc aggaacgcgc tgcacggcgg
      301 ttcgcacggg ctggccgtta cccgtttcaa cgcggtggcg gcagacggcc gttcggtggt
      361 gctgcgcagc cgcctgcaac agtcggccga cggttatccc aacgatttgg atttggatat
      421 ttcctaccgc ttggacgagg acaaccggct taccgttagc tatcgcgcca ccgcgctcgg
      481 cgacacggtg ttcgacccga cgctgcacat ttactggcgg ctggacgcgg gcctgcacga
      541 tgcggttctg catattccgc agggcggaca tattccggcc gatgccgaaa aactgcccgt
      601 ctcaacggtt tcagacgacc tcgaagtatt tgatttcagc cggcccaagc cgctggatgc
      661 cgccgttgcc gccctgcgcc gcgaaacggg tcgggccggt tttgacgacg cttaccgcgt
      721 gccgtccgat ataggccgtc ccgccgctgt gttgcaagcc ggacgccgcc gtcgtatcag
      781 catatacagc gaccgcaacg gcttggtcat ctttaccgcc gccccgcagg atttcgcgcg
      841 gcacgatgcg ggcgtttacg acgcgctggc gaccgaggcg cagacgctgc ccgacagcct
      901 gaattggccc gagttcggca atattcgtct gaacaagggt gataccaggg aggcgacgat
      961 tgcttacggc atcgaatccc tttcttagga gcttcctaac accggttgca gacggccttt
     1021 ttatagtgga ttaacaaaaa ccggtacggc gttgcctcgg cttagctcaa agagaacgat
     1081 tctctaaggt gctgaagcac caagtgaatc ggttccgtac tatttgtact gtttgcggct
     1141 tcgtcgcctt gtcctgattt ttgttaatcc actataagat ttcaccattc cctcaaatca
     1201 atccaaacag gagcttcata aatgtacaca agaattatgg aaatcagccc ttggacgctg
     1261 cgttcggcaa agctggaaaa agaacacaaa cggctgcaag agagcctgac cagtttgggc
     1321 aacggctata tgggtatgcg cggcagcttt gaggaaacct actccgccga cagccacttg
     1381 ggcacctaca tcgccggcgt gtggttcccc gacaaaaccc gcgtcggctg gtggaaaaac
     1441 ggctatccca aatatttcgg caaagccatc aacgcgctca atttcagcaa agtcaaaatc
     1501 tttgtcgatg gtcaagaggt ggacttggcg aaaaacgatg ttgccggctt ctccgtcgaa
     1561 ctcgatatgc agcacggcgt gttgcgccgc tcgttcaccg tattcggcgt gcgtttcgat
     1621 gtgtgcaaat tcctgtccgt cgcacaaaaa gagctggctc tcatccgctg ggaagccgta
     1681 tctatcgacg gcaaaaccca ccaagtccgc atcgattcca tcatcgatgc cgacgtgaaa
     1741 aacgaagatt ccaactacga agaaaaattc tggcaagtat tggacaaagg cgtttcagac
     1801 gaccgctcct acattgccac ccaaaccgtc accaatccct tcggcgtgga acaatttatc
     1861 gtcaacgccg agcaaacttt cgccggcagc ttcaaagccc tcggcggcag ccaaaccgac
     1921 tggcaggtct ccaattcttt cgaggccgaa gtcggcggca cgcccaaaac ctttgaaaaa
     1981 cgggttattg ttaccaccag ccgcgattat cagggcttgg aagcagtgaa agccgcaggc
     2041 cgtgccttgt cggaaaaagt cgcaggcgtt gcgtttgaaa ccttgctgga cgcgcacaaa
     2101 gcaggctggc tgcaccgttg ggaaatcgcc gacgtggtca tcgaaggcag cgacgaagcg
     2161 cagcaaggca tccgtttcaa tctgttccaa ctgttctcca cctactacgg cgaagatgcg
     2221 cgcctgaaca tcggccccaa aggctttacc ggcgaaaaat acggcggcgc gacctattgg
     2281 gataccgaag cctatgccgt accgctctac ctcgcactgg cggaacccga agttacccgc
     2341 aacctgctgc aataccgccg caaccaactg ccgcaggcgc agcacaacgc gcgcgaacag
     2401 ggcttggcgg gcgcactcta tccgatggta acgtttacgg gtatcgagtg ccacaacgaa
     2461 tgggaaatca ccttcgagga aatccaccgc aacggcgcga ttccttacgc catctacaac
     2521 tacaccaact acaccggcga cgagggctat cttgccaaag aaggcttgga agtcttggtc
     2581 gaagtgtccc gcttctgggc ggaccgcgtc cacttctcca aacgcaacgg caaatacatg
     2641 attcacggcg taaccggccc gaacgaatac gaaaacaaca tcaacaacaa ctggtacacc
     2701 aacaccctcg ccgcatgggt attggactac acccgcgaag ccttggcgaa atacccgcgt
     2761 ccggatttga acgtgcgtgc cgccgagttg gaaaaatggg cggacatcag cgcgaatatg
     2821 taccgtccgc atgacgaaga actcggcgta ttcgtgcaac acgacggctt cctcgacaaa
     2881 gacatccgcc ccgtgtccgc gctttcgccc gacgatttgc cgctcaacca gaaatggtcg
     2941 tgggacaaaa tcctgcgttc gccctttatc aaacaggcgg acgtattgca aggcatctat
     3001 ttcttcggcg accgtttcaa tatcgacgaa aaacgccgca atttcgactt ctacgaaccg
     3061 atgaccgtgc acgaaagctc gttgtcgccg tgtatccact ctattctcgc cgccgaactg
     3121 ggcaaagaag aaaaagccgt ggaaatgtac cggcgcaccg cacgcctgga cttggacaac
     3181 tacaacaacg acaccgaaga cggcctgcac attacttcta tgaccggctc gtggctcgcc
     3241 atcgtccaag gtttcgccca aatgaaaact tggggcggta aactcagctt cgcaccgttc
     3301 ctgccgagcg catggacagg ctacgccttc catatcaact accgaggccg tctgattaaa
     3361 gtcgccgtcg gcaaagaaaa cgtcgtcttc accctgctca aaggcgagcc gctcgatttg
     3421 caggtgtacg gcaaagacat cacactcaac ggcagccata ccgccgcgtt ggaaaaataa
     3481 ggagggcgca aaatgacgtt taccgcagtg ctcttcgatc tcgacggcgt catcaccgac
     3541 accgccgaat accactaccg cgcgtggaaa aaactcgccg aagaactggg catcagcatc
     3601 gaccgcaagt ttaacgagca gctcaaaggc gtgtcgcgcg acgattcgct caaacgcatc
     3661 ctcgcgcacg gcggcaaaac cgtcagcgaa gccgagttcg ccgaactgac ccgccgtaaa
     3721 aacgacaact acgtcgagat gattcaggca gtcaaacccg aagacgtgta tcccggcatt
     3781 ttgcccctgc tggaaacatt gaaagcaaac ggcaaaaaaa tcgccctcgc gtccgccagt
     3841 aaaaacggcc cgttcctgct cgaacgcatg gggctgaccc acttcttcga caccgtcgcc
     3901 gaccctgccg ccgtcgcgca ttccaaaccc gcccccgaca tcttcctcgc cgccgccgag
     3961 ggcgtgggcg cggacatccg ccgctgcatc ggcattgaag acgctgccgc aggtgtcgcc
     4021 gccatcaaag cctccggcgc cttacccatc ggtgtgggca aagccgaaga cttgggtagc
     4081 gacatcccgc tggtgtccgg caccgccgag ctgacctacg cctacctgca aaacgtgtgg
     4141 gcacagtcgg gcaggtaaaa cgcgtcagat aaagtgttaa ggaagtaaaa ggccgtctga
     4201 acagtgtttc agacggcctg agacctttgc aaaattcctg tccctcccga cagccgaaac
     4261 ccaaacacag gatttcggct gttttcgtac caaatacctc ctaattttac ccaaatatcc
     4321 ccttaatcct gcccggatac ccgataatca ggcatccggg ctgcctttta ggcggcagcg
     4381 ggcgcactta acctgttggc cgctttcaac aggttcaaac acatcgcttt caggtggctt
     4441 tgcgcactca ctttaatcag tccgaaatag gttgaccgcg catagcggaa tttacggtgc
     4501 agcgtaccga agctctgttc gaccacataa cgggtcttcg acaaataccg gttgcgtttg
     4561 gtttgcg
//
LOCUS       DAWFAD010000141         4509 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWFAD010000141 DAWFAD010000000
VERSION     DAWFAD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.141"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(127..1269)
                     /locus_tag="WGO49_001721"
     CDS             complement(127..1269)
                     /locus_tag="WGO49_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ0306801.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTAGRILLRLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKIEAKR
                     ANVKSLQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIAARRA
                     PGMMRRFGFEKLQNFDKTLWSDLRRRAEAQVKPAFAPISGSDSDRRIIQTALANARSA
                     GVDDIVSFSVADAQSVRPNGVGGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WQVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1318..1740)
                     /locus_tag="WGO49_001722"
     CDS             complement(1318..1740)
                     /locus_tag="WGO49_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306802.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1733..2542)
                     /locus_tag="WGO49_001723"
     CDS             complement(1733..2542)
                     /locus_tag="WGO49_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0306803.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCNDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2624..3001)
                     /locus_tag="WGO49_001724"
     CDS             complement(2624..3001)
                     /locus_tag="WGO49_001724"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ0306804.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3201..3968)
                     /locus_tag="WGO49_001725"
     CDS             complement(3201..3968)
                     /locus_tag="WGO49_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0306805.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFNMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSIFGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3978..>4509)
                     /locus_tag="WGO49_001726"
     CDS             complement(3978..>4509)
                     /locus_tag="WGO49_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEZ0306806.1"
                     /translation="DITFSEDVGECPLHSDSSFSENPESYLVMYVVKSANDGGNSLFL
                     SSSDIVNQLSKTETGKKHLKTLTGNLYPFKAPASFDKKQGVRWGNILSVNTQMIRFRS
                     DCIYKGIEENRNKVSKEMVLALDYLVKIIKNASDIQEFSAQDDGLIIIDNVNGLHAGT
                     DYTDKNRHYIRARITV"
BASE COUNT         1106 a         1130 c         1096 g         1177 t
ORIGIN      
        1 cgtactattt gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
       61 aatatcggcc ggatattaaa aaaagccgtc tgaacatttt tcagacggct tttttgtaca
      121 ctttcctcaa cggttcgatc cctgcaccat atcaatcagg aacaggcggc agtcgaggtt
      181 gccgttataa agcggaattt tccgcttggg cgacaggcgc atgaatttgg gcatttccct
      241 gtcgccggta aacataccga cctgccagcc ggcatagtgt tttttcaacc acgtccccaa
      301 ctgcggatac agtgcctgca aagcgcggac ttcctcaagg cgcacaccgt agggcggatt
      361 ggacaccata atgccgccca cgccgttcgg acggacggat tgcgcgtcgg caacgctgaa
      421 ggaaacgatg tcgtccaccc cggcactgcg tgcgttggcc aatgccgtct gaatgatgcg
      481 gcggtcgctg tcgctgcccg aaatcggtgc aaaagccggt ttaacctgcg cttcggcacg
      541 gcggcgcaaa tccgaccaca gcgttttatc gaaattttgc agtttttcaa aaccgaaacg
      601 gcgcatcata cccggcgcgc ggcgggcggc aatccaagcg gcttcgatag caatcgtgcc
      661 gctgccgcaa aacgggtctt gaaacggctg cgtgccgtcg tagcctgccg agagcagcaa
      721 tccggcggca aggttttcgc gcagcggggc ttcgccggta tcgagacggt agccgcgttt
      781 gaacagggct tcgcccgaag tgtcgatgaa gatttcgaca ttgcgttcgt ccaaaaaggc
      841 gtggatgcga acatcgggcg cggctttgtc cacgctcgga cgtgcgtcgt aaatgtcgcg
      901 gaaagcgtcg cagacggcat ctttgacggt cagtccgaca aattggaggc ttttgacgtt
      961 ggcacgcttt gcctcgattt tgactttgaa cgtctgctgc aaggtaaacc agttgaacca
     1021 gttgatgttt ttggcaagtt tgtaaatgtc gcgctcggtt cggtatgtgc ctttggtcag
     1081 gcgtagcagg atgcggccgg cggtacgcga gtgcaggttg gcggcgtaaa cctgttccaa
     1141 tccgccccgg caggaaacgc cgccgtcaaa cacttgtaca tcggtacagc cgaggctttc
     1201 gagttcttga gataaaacgg tctccaagcc gcgcgggcag gtggcgaaaa gtgtatataa
     1261 agtattcata acgtgtcctt tccggacgga tgttccggca atggcggatt gaaaggttta
     1321 ttcggcggta aatgccgtct gaaatgtccg actgccgatt gtaatgtccg ccgtaaaatc
     1381 gcgcctgcct tcgacacaga tgggcaggcg gatgcgtact gcctgccaag tccccgacgg
     1441 ttgccgctcg aacatatagc ggttgaaacc catatccata tttttcatac tgaagctgat
     1501 gctgacctgt tccgtgccgg cgggcgcgtg ttcgatataa atatcaaacg gttttttggt
     1561 tgaaacggcg gcggcgcgga cgcggcttcc gtccggcagc gtgcaaccct cggtcaaatc
     1621 gcattgcgcc gccacagctt gcggctgctg cgcctgccac cattgcaaca aaacgagctt
     1681 gacggcggca aaggcaatca gcagcagtgc ggcaagcagt aatttacggt ttttattcat
     1741 gattcttccc tattgtttca tcccttacca ccacgtgcgc ccccaatccg agccatagtt
     1801 ttccataacg tgcaaccgtc tgtccgtttg ctgcaagtac gagcggcacg gatacgccct
     1861 gccgcagggc ttctgccgcc tcttgtgcct gaacatcatt gcacacgcgc cacacgacaa
     1921 catccgcgcc cttttcctgc gcctgccgcc attgttcgcg ggtctgcacc atcatccaca
     1981 cgctgtcggc gtgttcgggt ctgtcctgcc attgcgccgc ctccatcaaa acgttcgcac
     2041 cgcttccctc tgccgaaccc aaaggcagga cgcggtacgc gcccatactg ttctccccgt
     2101 gcaaacccgt tttcaggctg ccgtacaaac ggcgcggaac ggacagcgaa cgcaaaagcg
     2161 cgccgttggc gggcagcatg ggggcaacgg taaaatcacc cgccttctgc caagaccatt
     2221 cctgcccttc gcgggattgc ggttcgcccg tccattggcc ggggttgacc cataggaatt
     2281 tcaggcagac gcgggcgtgt tcgtaggaat ggattttggt cagccaaggc gtggcggcaa
     2341 ggatgcggat gccgagttct tcttcaaact cgcgttgcaa ggcttggaag tcggtttcgc
     2401 ccgcttcgac cttgccgccg gcaaattccc aatatccggc atagggtttg ccttcggggc
     2461 gcgagctgag caggtagttg ccgtctgaat cgagcaggat gccggcaacg acgcggataa
     2521 ggggtcgggt gtcttgaatc atggcgggta cgcggttggt cggtcggagt gcaaatgagg
     2581 gacgagcaat gccgtcaacg ggtttgccgc atttcagacg gcattatttt tcggcaacga
     2641 caaacgccaa tacggtgtct tcttcgtcgc tcatgctgag gctgacgcgg ctgatgcctt
     2701 gttcctccag ccatttggac agggcggggc cgtagaaaaa ttcgggcttg cccaatgcgt
     2761 catgcccgat gccgatgttg cggaaggaaa ccgcgccgcg tatgcccgtg ccgacggctt
     2821 tggcgaaggc ttctttggcg gcaaagcgtt tggcgaggta gttgacgggt ttgcccgctt
     2881 gcggaaattc aagcagctct tccggagtga ggatgcgccc ggcaaacgcc tgtccgaatt
     2941 ttttgtttaa gcggatgatg cgcttgaggg aaacaatgtc tgtgccgatg ccgtaaatca
     3001 tatttgcgct ccttcgccct tggttgcggg tagtgatgaa tgtgatgatg aatgccgtca
     3061 atttcttggg gcgttaaaat ggcgtgaata tccacttttg gggttttgtc ggtaacaatt
     3121 ttcactgtaa ccttttgcat ttgaaattct aaaacatcgt gccaattatt taataaggag
     3181 ttaaggcatt taataatgaa ttaaagtttg attaaacccg aatgcccgat tttcagagcc
     3241 aataccaata aaacaataaa aattaacatt ttaaaaaaat ttgggctaat cttagtcctt
     3301 aaccgaattc caacatacaa tccaataaca gacaatacgg acagtaaaaa tattaaaccg
     3361 tattcactct tatttaataa ccaatactgg tctcttagca tatatatttg aacaattttc
     3421 gccaaaagat agcatagatt gcttgatttt acgatacgat ttttattttc tgtttcgcta
     3481 agcaaaaata ttaacaatat gggagacatg gcattggttg aaccgccgat gatgcctgcc
     3541 aaaaacccaa aaagaaccat attcttatta ttggcaacta cttgaatatt ttttgctttt
     3601 gcacatacat ttaaaatacc attgacagaa taatacaatg taatgattgc catcagtaaa
     3661 agcagccaag acactggaag tatcaaaagc aacttcaccc caaaaatgct gccaacgacg
     3721 ctgccgatag caagcaattt ataggttttt aaataataaa caatctcttg ccaaaaacct
     3781 tttttgttat tgctgcatag aaccaacaag ctcattaaca ggcttggtaa tgccaccaag
     3841 gcaacaacct tagacaatgg catgttaaaa gccaatgcgg ttgtaccgag catcggaaat
     3901 cccatgcctg taattccgtg cagtattgcg gcagcaacaa aaacgataga ttgcattatt
     3961 tcttgcataa ccgtccttta tacagtaatt cttgctctaa tataatgcct gtttttatcc
     4021 gtataatcag ttccggcatg caagccattg acattgtcaa taataatcaa accatcatct
     4081 tgtgcagaaa attcttgaat atcactcgca ttttttataa tctttacaag ataatcaagt
     4141 gcaagtacca tttcctttga tactttattt ctattttctt caataccttt atagatacaa
     4201 tcacttctaa atctaatcat ttgagtattg accgataaga tattacccca tctcacacct
     4261 tgttttttat caaatgatgc tggtgcttta aatggatata aattgcccgt taatgttttt
     4321 aagtgttttt taccggtttc tgttttagat aactgattga caatatctga tgaacttaaa
     4381 aatagggaat tacctccatc attggctgat tttactacat acataaccaa ataactttcc
     4441 gggttttcac taaaagatga atcactatga agtggacatt ctccaacatc ttccgagaaa
     4501 gttatatca
//
LOCUS       DAWFAD010000142         4477 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWFAD010000142 DAWFAD010000000
VERSION     DAWFAD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.142"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            281..1402
                     /gene="dnaJ"
                     /locus_tag="WGO49_001727"
     CDS             281..1402
                     /gene="dnaJ"
                     /locus_tag="WGO49_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ0306807.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1598..3616
                     /locus_tag="WGO49_001728"
     CDS             1598..3616
                     /locus_tag="WGO49_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ0306808.1"
                     /translation="MNKSLSGSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMIIFARRVLKAGR"
     gene            complement(3660..4217)
                     /gene="rfbC"
                     /locus_tag="WGO49_001729"
     CDS             complement(3660..4217)
                     /gene="rfbC"
                     /locus_tag="WGO49_001729"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0306809.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMRKDSPTFGKWVGEILS
                     AENKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPEAEHSLIWNDPTVGIDWPLQGE
                     PNLSPKDLAGKTWAQAEKLRLTLSR"
     gene            complement(4271..>4477)
                     /locus_tag="WGO49_001730"
     CDS             complement(4271..>4477)
                     /locus_tag="WGO49_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEZ0306810.1"
                     /translation="WLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDE
                     KLEELARPMAKNQYGQYLLRLLKK"
BASE COUNT          936 a         1013 c         1326 g         1202 t
ORIGIN      
        1 gataaaaaat aaaataaggg gaagtctgca gccgcatcaa atgccgtctg aacatgcgtt
       61 cgggcggcgt ttttataaca aaaacacttc atggcggttg gttttatgcc tgtctaagtt
      121 tttgtgtcgt gcatacctga agatttcaga cggcatcggt ttatgctgtc tgaaaagtgt
      181 attccgtttc agtttgtaag ctatggcagt ctgtttgtct tgtgttttgc gcaattgccc
      241 ttattttgag ccgtgatttt attttgaatt agatgaaaaa atgagtaatc aagattttta
      301 tgcgacgctg ggtgtggcaa gaacagctac cgatgatgag attaaaaaag cctaccggaa
      361 attggcaatg aaataccatc ccgaccgcaa tcctgacaat aaagaggcgg aagagaagtt
      421 taaagaagta caaaaggcgt atgaaacttt gtccgacaag gaaaagcgcg ctatgtacga
      481 ccagtatggt catgcggcgt ttgaaggcgg cggacagggg ggcttcggag ggtttggcgg
      541 atttggcggt gcgcagggtt ttgactttgg ggatattttc agccaaatgt ttggaggcgg
      601 ttcggggcgc gcccagcctg attatcaggg tgaggacgtt caagtcggta tcgaaatcac
      661 gcttgaagaa gccgcaaaag gtgtgaagaa acgcatcaat attccgactt atgaagcgtg
      721 tgatgtctgt aacggcagtg gcgcgaaacc ggggacatcc ccggaaacct gcccgacttg
      781 caaaggttcg ggtacggtgc acatccagca ggcgattttc cgtatgcagc agacttgtcc
      841 gacctgccac ggtgcgggca aacacattaa agaaccttgc gtcaaatgcc gtggcgcggg
      901 gcggaataag gcggtcaaga cggtggaagt caatattccc gccggtatcg atgacgggca
      961 gcgtatccgt ttgagcggcg aaggcgggcc gggtatgcac ggtgcgcctg ccggcgactt
     1021 gtatgtaacc gtccgcattc gggcgcataa gattttccaa cgcgacggtc tggacttgca
     1081 ttgcgaactg ccgatcagtt ttgccacggc tgctttgggc ggggagttgg aagtgccgac
     1141 cttggacgga aaggtcaagc tcaccgtccc caaagaaacc caaaccggca ggaggatgcg
     1201 cgtgaagggt aagggtgtca aatctttacg cagcagcgcg accggcgatt tgtactgcca
     1261 tattgttgtc gaaacgcctg tcaatttgac cgaccgtcaa aaagagcttt tggaagaatt
     1321 tgagcggatt tctaccggct tggaaaacca aacaccgcgc aagaaatcgt ttttagacaa
     1381 gctgcgcgat ttgtttgatt gattttaagg ttcggaaaca agcagccgta tcggggaatc
     1441 tccctgatac ggctgttttt gtttgtttaa aaatagtttt tattttcaat ggggtatggg
     1501 gcagggtggg ataactgttt ttaactgttc tttttaaaac ttgacatcat ggcgcgatgc
     1561 caacaatatg tgaacgtctg ttgtcaaagg aagaataatg aataaatctt tatccgggtc
     1621 ggtagaagaa taccgcgaac tgacgctccg aggcatgata ctcggtgcat tgatcactgt
     1681 aatttttact gcgtccaacg tttacctcgg tttgaaagtc gggctgacct ttgcctcgtc
     1741 gattccggcg gcggtgattt cgatggcggt tttaaagttt ttcaaaggca gcaatatttt
     1801 ggaaaacaac atggtgcaga cccaagcttc ggcggcgggt acgctttcga ccatcatctt
     1861 cgtcctgccc ggtttgctga tggcgggcta ctggagcggt ttcccgttct ggcagacgac
     1921 gcttttatgt attgccggcg ggattttggg ggtgattttc accattccgc tgcgttacgc
     1981 gatggtggtg aaaagcgatt tgccttatcc ggaaggtgtg gcggctgctg aaattttgaa
     2041 agtgggcggt catgaagaag gggataaccg tcagggcggc agcggcatca aagagctggc
     2101 ggccggcggt gcgttggcgg gattgatgag cttttgcgcc ggaggtctgc gcgtgattgc
     2161 cgacagcgcg agttattggt ttaaaagcgg tacggcgatt ttccagctgc cgatgggctt
     2221 ttcactggca ttgttgggcg cgggctattt ggtcggactg acgggcggta tcgccatcct
     2281 gttgggcatt tcgattgctt ggggcattgc cgtgccgtat ttctcctcac acattccgca
     2341 accttccgat atggaaatgg cggcgtttgc gatgaagctg tggaaggaga aagtgcgttt
     2401 tatcggtgcg gggactattg gcattgcggc ggtttggacg ctgttgatgc tgctcaagcc
     2461 gatggtggaa ggcatgaaga tgtcgttcaa gagttttggc ggcggtgcgc ccgctgcgga
     2521 acgcgccgaa caggatttgt cgcctaaggc tatgattttt tgggtgctgg cgatgatgtt
     2581 tgttttaggc gtgtcgtttt accactttat cggcgattcg cacattacgg gcggcatggc
     2641 ttggcttttg gtggtcgttt gcacgctttt ggcttccgtc atcggctttt tggtcgccgc
     2701 cgcctgcggt tatatggcag gtttggtcgg ctcgtcttcc agcccgattt ccggcgtggg
     2761 catcgtgtcc gtcgtcgtta tttcactggt tttgctgctg gtaggcgaat ccggaggttt
     2821 gttggcggat gaggctaacc gcaaattttt gctggcactg actttgtttt gcggctcggc
     2881 agtaatctgc gtggcttcga tttccaatga caacctgcaa gacttgaaaa ccggctacct
     2941 gctcaaagcc acgccttggc ggcagcaagt cgccctgatt atcggctgta tcgttggtgc
     3001 gctggttatt tcgcccgtgt tggaactgct ttacgaagcc tacggcttta ccggcgcaat
     3061 gccgcgcgaa ggcatggatg cggcgcaggc tttggcagcc cctcaagcga ctttgatgac
     3121 gaccatcgcg tcgggcattt tcgcccacaa ccttgaatgg gtctatatct ttaccggcat
     3181 cgcgattggc gcggtgttga ttgccgccga tttggtgttg aaaaaatcat caggcggcaa
     3241 acttgccctg cccgtccttg cggtcggtat gggtatttat ctgccgccgt ccgtcaatat
     3301 gcccatcgtg gcaggcgcgg tgttggcggc ggtgttgaaa cacatcatcg gtaaaaaagc
     3361 ggaaaaccgt gaaggccgtc tgaaaaacgc cgagcgcatc ggaaccttgt tctccgccgg
     3421 tctgattgtc ggtgaaagtc tgattggtgt gattatggcg tttattattg ccttctccgt
     3481 gaccaacggc ggctcggacg cgccgctcgc gttgaatctg caaaactggg atgccgccgc
     3541 ttcttggctg ggcttggcgt tcttcgttac cggaatgatt atttttgcac ggcgcgtact
     3601 gaaggcgggt aggtagcctg tcggagaaaa tgccgtctga aacgttcaga cggcattttt
     3661 tatcgggaaa gtgtaaggcg gagcttttcg gcttgcgccc acgttttgcc ggcaaggtct
     3721 ttgggcgaca ggttcggctc gccttgtagc ggccagtcga tgccgacggt cggatcattc
     3781 caaattagtg agtgttcggc ttcagggttg taatagtctg tgcatttata gacgaactcg
     3841 gcttcatcgc tcagtacata gaagccgtgt gcgaaacctt cgggtaccca cagttggcgt
     3901 ttgttttctg cggacagaat ctcgcctacc catttgccga aagtggggga gtctttacgc
     3961 atatcgacgg ccacgtcgaa tacttcgccg acaactacgc gtacgagttt gccttgtgtg
     4021 ttttcggttt ggtagtgcag gccgcgcaat acgcctttgc cggatttgga gtggttttcc
     4081 tgcacgaagg tgcgttcgca gacttgggtt ttaaaccact cgtcgcggaa ggtttccata
     4141 aaaaagccgc gcgcgtcgcc gaagacttgg ggctcaagca gttttacgtc aggaatggcg
     4201 gtatcaatga tgttcatctt tttatctttc atctaaaggc cgtctgaaaa gtttcagacg
     4261 gcctcaaaca ttattttttc aacaggcgca gcaaatattg gccgtattgg tttttcgcca
     4321 tcgggcgcgc caattcttcc agtttttcat cggaaagcca accgttgcgc caagcgattt
     4381 cttccaggca ggcgatgtgc aggttttgga tgttttgcac ggtttggacg aacgaagccg
     4441 cctcgtgcag gctttcgtgc gtaccggtgt ccagcca
//
LOCUS       DAWFAD010000143         4369 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWFAD010000143 DAWFAD010000000
VERSION     DAWFAD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.143"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(35..1414)
                     /gene="norM"
                     /locus_tag="WGO49_001731"
     CDS             complement(35..1414)
                     /gene="norM"
                     /locus_tag="WGO49_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ0306811.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFIDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGTVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVLWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            1749..2900
                     /locus_tag="WGO49_001732"
     CDS             1749..2900
                     /locus_tag="WGO49_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0306812.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGTVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALDELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTSERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            3004..4302
                     /locus_tag="WGO49_001733"
     CDS             3004..4302
                     /locus_tag="WGO49_001733"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ0306813.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAILAYYNVQLQHLHNAEPVKAEDVMAVIEKVALRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMPGWRESTFGVKSYDA
                     LPANAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
BASE COUNT         1045 a         1197 c         1249 g          878 t
ORIGIN      
        1 tgttaatcca ctatattttt aatcggcgga gttttcagac agccttatgc gatctgacca
       61 tctccctact gcacaattcc aagcaccaca ccaaggcgat ggcggcgatg gtgagcgagg
      121 caatcaatgc cgtccagaag ccgtaaatgc ccatatcgaa acggtaggcg agcaggtagc
      181 ccggcagcag accgcagccc caaaaggcgg cggcgtggat gaacatcggc acctttgtaa
      241 ctttgtagcc gcgcaaggcg taggaggcga tacattgggt gaagtctgcc ggttggaaca
      301 agccggcgaa cagtaagacg gtggcggcga tgcttaaaac cgccggatca ttgttgtaca
      361 tacttaccag cggcgaacgg aataatacca aggaaagcac ggtaatcacg gcgagcatcc
      421 atcctaacac cagtgacacg cccgaaatat aacgcgcccg cgaaaattcg cgccgcccaa
      481 gcgaaaagcc gatgcgcacc gtccccgccg agccgacgct ttgcggaatc atatagagaa
      541 tccccgacaa actgatgccg acctgctgcg ccgccacata atcctcgccg aaaggcgcaa
      601 tcaaaaacac gataaacgaa aacgcgctgg cttccaaaaa ataagacagc ccgatgggtg
      661 cgccgatttt ccaaatctgt ttgaacaccg cccaatccgg tttgccgaat ttcgccgtca
      721 gtccgaatgg gcggaagaaa ttttccttgg cgatataaat ccacaatgcc agcgcgctga
      781 accacaacac tgccatcgtc gccagtccgc agcctgcgcc gcccaaagcg ggcataccga
      841 atttgccgta aacgaaaata tagttcagcg gcacgttcaa cacaaacgcc gcaaagctga
      901 ccaacataat caggcgcggg cggttcaggc tggaggcgta ggcgtgcagt gcgcggtgta
      961 ccattgccgc cggcatcgcc aagctggtga acagcatata ctgcgccatt gtgccttcca
     1021 cataatcgct caaggtcagc cagttgcgga acggcgtaat cgccgcccac atcaataccg
     1081 tgccgaacac gcccaaaaac agcccgaacc aaatcccctg ccgccccgtt tcgcccactt
     1141 cgtcggtttt acccgcgccg taaagctggg caatcatcgg gttcagcgcc gccataatgc
     1201 ccataaaggt aatataaacc gtggcaaacg cgctgctgcc caaagccacc gccgccaagt
     1261 cttccttgcc tgcaccaccc gccatcacag tatcgataaa accgatgccc acctgcgcga
     1321 cctgcgccaa cagcatgggc agggcaaggg cggtcagcag gcggacttct ttcaggaaga
     1381 cggaaaagga aaagcggttg aggtcgagca gcataagtgt tcaatcaaca aaaatgccgt
     1441 ctgaagcaga aaacggcagc aggaagtgat gagaaataat ggtgcacatt atatcgtaaa
     1501 aaaatgctgt gccgtcagac ggcggataca gggtataaaa gtatattcag attgtgtgta
     1561 ttttatggta aagtttggtt ttaacgactt gacggcattg agccgtcgga caggggctgt
     1621 tcggattctg aatcggaagg aagcatcgcc gttttgacag cggcgtgatg cgttgcggca
     1681 aagatgccgt ctgattgcgg atcgggcagc cttttgtatt tacatgataa aatagaaggc
     1741 agattctcat gcagacatgg cagcttccgg aacatatcgc cgacgtactg cccacgaacg
     1801 cgcggcagct tgaaagcgcg agggagcagt tgttggcact gttccgcgta cacggttatg
     1861 aactggtaca gcctccgctg atggagtacg cacattccct gctgacgcat atcgatgcgg
     1921 ggctttccct gaaaaccatt ttggtaacgg acaggctcag cggcaggcag ttgggcatac
     1981 gcgccgacat cacgccgcag gtggcgcgta tcgatgccca tcttttatcc gccaaccaag
     2041 ggattaatcg gttgtgttat gccggtccgg tgttgcacgc gcagcccgac ggtctgctga
     2101 atatgcgcga acccttgcag gcaggggcgg aaatgtacgg ttttgccgac atccgtggcg
     2161 acatcgagct gatagacctg atgctgaaaa gcatgaaaat tgccgatatg ggcaaagtgc
     2221 tgctttcgct ggggcatatc ggcatatttc gcgccttgtc cgatgcggcg catttggatg
     2281 cggggcagtc tgcaacgctg cttgccttga tgcaggataa agataccggg acggtcgaag
     2341 cgcaggtcaa ggcttggaag ctggacggca tgtgggcaaa agcattctcg ctgctgccgc
     2401 gcctgtacgg cgggcgtgaa gtgttgtccg acgcgcgcgg acggttgccg gatttgtcgg
     2461 cggtcggcgg cgcgttagac gaattgcagg cggtgtgcga cgcattcccc gattgtgaaa
     2521 tccatatcga cttgtccgag ctgcgtgtcg acaattacca cacgggcttg ctgtatgccg
     2581 cctatgccgc cgatttccac gacgcggtcg cgcgcggcgg gcgttatgac ggattgggcg
     2641 gatatttcgg cagggcgcgc ccggcaacgg gattcagttt cgacttgcgc agctttatcg
     2701 ggcgtttgcc caccagcgaa cggcagcccg ccgtgttggt cgatgcggaa gatgccgaag
     2761 cggcgcgcga agcagtcgaa gccttgcgtg aacaagggca gtgtgtcgtg attgactacg
     2821 gtatcggaca caatgtttcg gaagagcttg caggccgtct gaaaaagacg gacggcgttt
     2881 ggcaggtcgt gaaacgctaa atacccgttc atggcggatg aaaggcaaat cgtggtgggg
     2941 cgcaaagccg caccggtttg gggatttccg caataatttt taatatcgat aggttatatg
     3001 gctatggcta aaaatgttgt agtaatcggc gcacagtggg gcgacgaggg taaaggtaaa
     3061 atcgttgact ggctggcgga agaagccggc ggcgtggtgc gcttccaagg cggccacaat
     3121 gcgggtcata ctttggtcgt cggcggcaaa aaaaccattt tgcgcctgat tccgagcggc
     3181 atcctgcatg aaagtctgga ctgcttcatc ggttcgggcg ttgtcgtctc ccccgaagcc
     3241 ctgttgggcg aaatcgacga gttaaacgct gcaggcgtga aaaacgtcga aggccgtctg
     3301 aaaatcgcgc cgacctgccc gctgatcctg ccttaccaca tcgcgctcga ccaagcccgc
     3361 gaagcatcgc gcggcaaagg caaaatcggc acgaccggcc gcggcatcgg ccctgcctac
     3421 gaagacaaag tggcacgccg cgccattcgc gccgccgatt tgctgcatcc tgaaaaactg
     3481 cgtgaaaaac tggatgccat ccttgcctat tacaatgtcc aactgcaaca tctgcacaat
     3541 gccgagccgg ttaaagcgga agacgtgatg gcggttatcg aaaaagtcgc gctgcgcatt
     3601 gcgccgatga ttaccgacgt gtcccgcgtg ttgaacgaga aaaacaaaaa cggcgaaaaa
     3661 ctgctgtttg aaggcgcgca aggtgcgttg ttggacatcg actacggcac ttatcccttc
     3721 gttacctcgt ccaactgtct ggcgggagcc gcttccgcag gcgcgggcgt aggccctcaa
     3781 atgctggatt atgttttggg catcgtcaaa gcctatacca cgcgcgtcgg ttcaggcccg
     3841 ttcccgaccg aattgttcga cgaagtaggc gcaggtttgg cggaacgcgg acacgaattc
     3901 ggctcggtaa ccggccgcgc acgccgctgc ggctggtttg atgccgccgc cctgaaacgc
     3961 tccatccaaa tcaacggcat ttccggtatg tgcattacca aactcgatgt aatggacggc
     4021 gttgaaacca tcaatatctg cgtcggctac gaattgcccg acggcggcaa aaccgatatc
     4081 ctgccttgcg gttccgatgc ggtggaaacc tgcaagccga tttacgaaac catgcccggc
     4141 tggcgtgaat ccactttcgg cgtgaaaagc tacgacgcat tacctgccaa tgccaaagca
     4201 tatttgaaac ggattgaaga agtctgcggt gcgccggtcg ccatcgtctc caccggcccc
     4261 gaccgagaag aaacgattgt gctgcatcat ccgttcgcat aaggttttgc agtaaaaatg
     4321 ccgtctgaag cccctccgag ttcagacggc atttttgctg ccgttttat
//
LOCUS       DAWFAD010000144         4361 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWFAD010000144 DAWFAD010000000
VERSION     DAWFAD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.144"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(69..2045)
                     /locus_tag="WGO49_001734"
     CDS             complement(69..2045)
                     /locus_tag="WGO49_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ0306814.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WGO49_001735"
     CDS             2194..3033
                     /locus_tag="WGO49_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ0306815.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GETVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQDAAQN
                     TAS"
     gene            3232..4263
                     /gene="gap"
                     /locus_tag="WGO49_001736"
     CDS             3232..4263
                     /gene="gap"
                     /locus_tag="WGO49_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0306816.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSVLTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTVEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1103 a         1465 c         1015 g          778 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccgtttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacgggacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccaccca gcccgaacac cagccgcctc
     1801 attgccctca gcttgggcag cgaaaactcc aagcctatac tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcataggcc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgcattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctatgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga aaccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgtcgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat acctcaaaca atacaaagcc
     3001 aagcaggatg ccgcacaaaa caccgcctcc tgacccttcc ctgatatgcc gtctgaagcc
     3061 gcttcagacg gcattttatc aagctctccg tccgccgcac ccgtgccgtc cgcatcttac
     3121 cgcccaccct tccggccgcg ccgttttcaa taaaatatta attacacgcc actacaaatt
     3181 tgctataatc cgccccgaaa atctacccaa ccctcaacaa aggaacgaac catgggcatc
     3241 aaagtcgcca tcaacggcta cggacgcatc ggccgccagg ttttgcgcgc catctacgat
     3301 tatcagattc aagaccaact ccaaatcgtc gccgtcaacg ccagcggtag ccttgaaacc
     3361 aacgcccacc tcaccaaatt cgacaccgtg cacggacgct ttgaagccga cgtatcccac
     3421 gacggcggca acctcatcgt caacggcgac aaaatcccct tcttctcgac ccgcaaccct
     3481 gccgaactgc cgtggaaaga actcggtgtc gatttggtca tggaatgcac cggcgcgttt
     3541 accagcaaag aaaaagccaa aatccacctc gaaagcggcg cgaaaaaagt cctcatttcc
     3601 gcaccgggcg gcgacgatgt cgatgcaacc gtcgtgtacg gcgtgaacga cagcgtcctg
     3661 accgccgaca tgaccgtcgt ttccaacgct tcctgcacca ccaactgcct ctcgccggtt
     3721 gccaaagtgt tgagcgaaag cgtcggcatc gtcaaaggcg cgatgaccac catccacgcg
     3781 ctgaccaacg accaaaccgt taccgacgtg cgccacaaag acctgcgccg cgcccgcagc
     3841 ggcgtggaaa acatgattcc gaccaaaacc ggcgcggcaa aagccgtcgg tttggtactg
     3901 cccgaattga aaggcaggct cgacgggctt gccatccgcg tgccgaccgt caacgtatca
     3961 ttggtagatt tgagcttcca agccgcgcgc gacacgacag tcgaagaaat caacgcactg
     4021 atgaaagccg cctcggaagc aggcgcgctc aaaggcgttt taggctacaa caccctgccc
     4081 ttggtttcca tggacttcaa ccacactacc gaagccagcc acttcgacgc aacactgacc
     4141 aaagtcgttg acggcaacat gattaaagtc ttcgcatggt atgacaacga atggggcttc
     4201 agctgccaaa tgctgaacac cgcacgccgt atgttcggac ttgaagtgcg cccgctcaaa
     4261 taagcaataa accgtcaaac aaaatgccgt ctgaaacccg atgttttcaa gttccagacg
     4321 gcatttttca ccgtgatttt acccggctgt cgtcatttcc a
//
LOCUS       DAWFAD010000145         4307 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWFAD010000145 DAWFAD010000000
VERSION     DAWFAD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.145"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            94..365
                     /locus_tag="WGO49_001737"
                     /pseudo
     CDS             94..365
                     /locus_tag="WGO49_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            405..1439
                     /locus_tag="WGO49_001738"
     CDS             405..1439
                     /locus_tag="WGO49_001738"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ0306817.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPSADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1645..2226
                     /locus_tag="WGO49_001739"
     CDS             1645..2226
                     /locus_tag="WGO49_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ0306818.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2295..2477
                     /locus_tag="WGO49_001740"
     CDS             2295..2477
                     /locus_tag="WGO49_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ0306819.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALSEEELKA"
     gene            2474..3235
                     /gene="kdsB"
                     /locus_tag="WGO49_001741"
     CDS             2474..3235
                     /gene="kdsB"
                     /locus_tag="WGO49_001741"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ0306820.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSSLETIESLEQLRVL
                     WHGYSIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            3258..3575
                     /locus_tag="WGO49_001742"
                     /pseudo
     CDS             3258..3575
                     /locus_tag="WGO49_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            3680..3877
                     /locus_tag="WGO49_001743"
     CDS             3680..3877
                     /locus_tag="WGO49_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306821.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDAAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            4034..4126
                     /locus_tag="WGO49_001744"
     tRNA            4034..4126
                     /locus_tag="WGO49_001744"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4068..4070,aa:Ser,seq:gct)
BASE COUNT         1075 a         1039 c         1166 g         1027 t
ORIGIN      
        1 ttcagcacct tagagaatcg ttctctttga gctaaggcga gccaacgccg tactggtttt
       61 tgttaatcca ctataaatga aagatactgg caaatgaaag agatgaaacc tgtccgttat
      121 catattggcg atatgcccga gacttcaaaa caaaccgcct cccgtcatga cgacagggca
      181 gtgggtgttg acgatgattg ttttcatgat tcctttggtc aatttttgtt tgggtgttcg
      241 gcagaggcaa cccgaaccgc gccaatttct gtaaagcgca gttgcttatt tacctgattg
      301 gttcgcttat cggtttggtc ttcgcgttgt ttataggtgg gtctgtatcg ggtacgcatg
      361 attaatgccc cgggctgatt ttgcttcgag gatttgtatc gaatatgccg aattgtttca
      421 aatttcatac cgttatcgaa cggcattggc aaaaacctta tccggttttg tcttttctgc
      481 ttaagccgct ctccgggctg tttgccaaaa ttgcggcaaa acggcggacg gattttttat
      541 cgggaaaacg gcaaagcgaa aagctgcccg tgcctgtggt cgtggtcggc aatattcacg
      601 cgggtgggac ggggaaaacg ccgattgttg ccgcgctggt gtcgggtttg caggaaaagg
      661 gcgtcaaggt cggcatcatc agccggggct acgggcgcaa gagcaaggcg gttcatgtat
      721 tgaatgctga gagccgagcg gaagatgcgg gcgatgagcc tttgctgctg ttccgcaaaa
      781 ccggtgcgcc gacggcggtg ggcagcagcc gtgcagaggc aggcagggcg ttgctggcgg
      841 cgcatcccga catcggactg attgtggcgg acgacggttt gcagcattac gccctgcggc
      901 gagatgtgga aatcgcggtg tttccgtcgg cggatacggg gcgcacggat ttggatttac
      961 tgcccaacgg cagtttgcgc gaacctttgt tgcggctgga ttcggtggat gcggtcgtcg
     1021 tcagcggcgg caaggcggat gcgctgttta ggccgtctga aaatatgttt cacagccgta
     1081 tcgaagcggg acggatttac cgtttgaaca atccgtccga aatactggac acaggccgtc
     1141 tgaaaaatca aaccgtcgtc gccgtggcag gtattgccaa gccggcgcgt tttttcgatt
     1201 cgttgcggaa tatgggcatt accgtgaagc gaaccgtcgc gctgcctgac cacgccgaca
     1261 tttcggcggc agatttgccc gatgcggacg cggtcattat tacggagaaa gatgcggtca
     1321 aattttcaga cggcatttgc accgataatg tttgggtgtt gcccgtttgt gcgataatcg
     1381 aacctgattt ggcggcgttt gtgttggagc ggttggaaga tgtaccgaag gccgtctgaa
     1441 agcacggttt gggcggagtg attacggatt tgaataagaa cgcctcgcgc cgtcattccc
     1501 gcgcaggcgg gaatctaagt ctcgaatttt caggaatgcc taggaggctt cagaaatccc
     1561 aaatctccgg atttccactt ggacaggaat gagaaaatcg gtcgtatttt ttatctgcat
     1621 taatcattcg ttaaagtatt gaatattaaa ctgaaaacct tgttattgcc cttcgccacg
     1681 ctggcattgt gcaccaacgc ttttgccgcc ccgcccagcg acgcgtcgtt ggcgcgttgg
     1741 ctggatacgc agaattttga ccgggatata gaaaaaaata tgattgaggg ctttaatgcc
     1801 ggatttaaac cgtatgcgga caaagccctt gccgaaatgc cggaagcgaa aaaagatcag
     1861 gcggcaaaag cctttaaccg ctatcgtgag aatgttttga aagatttgat tacgcccgaa
     1921 gtgaaacagg ctgtccgcaa tactttattg aagaatgccc gtgagatata cacgcaagaa
     1981 gaaattgacg gcatgattgc cttttacggt tcgcctgtcg gtcagtccgt cgttgccaaa
     2041 aatccgcgct taatcaagaa atcgatgagt gaaatagcag tatcttggac tgcattgtca
     2101 gggaaaatcg cgcgacatca tctgcccgcg tttacggaag agttgcggcg catcatctgc
     2161 ggcggtaaaa atcccgatgc gggctgtaaa caagccggac aggttgggaa aaggcatcag
     2221 aaataaatga tagccgtctg aaatattgaa gagggcatcc gattgattga accatcaaac
     2281 ccgaaagcaa ccctatggaa aaaaaattct tagacatcct cgtctgcccc gttaccaaag
     2341 gcaggctgga atatcatcag gacaaacagg aattgtggag ccgtcaggcg aagcttgcct
     2401 atccgattaa agacggcatt ccctatatgc tggaaaacga agcgcgagcg ttgagcgaag
     2461 aggaactcaa agcatgactg aattcgtcgt attgattccg gcgcggctgg attcatcgcg
     2521 cctgcccgga aaagccttgg cggatatcca cggtaaaccg atggtcgtgc gcgttgccga
     2581 acaggcggca aaaagtaaag ccgtgcgcgt cgtcgttgcc accgaccatc ccgatattca
     2641 gacggcctgt caggcgcacg gcgtcgaagt cgtcatgact tcaaaccggc acgaaagcgg
     2701 cacgacgcgc cttgccgaag cctctgccgc gctgaagctg ccgccgcatc tggtggttgt
     2761 caacgtacag ggcgacgagc cgctgattgc ccccgaactc atcgaccgca ctgccgaagt
     2821 cttggtggaa aacaacgtac agatggcaac tgcggcacac gaattgcacg atttcgacga
     2881 attgatgaat cccaatgccg tcaaagtcgt cctcgacaaa aaccgcaacg ccatctactt
     2941 cagccgcgcc ccgattccct atccgcgcga tgcgatgcgt gcaggaaaac gcgaaatgcc
     3001 gtttgaaacc gccgtcctgc gccatatcgg catctacgcc taccgcgtcg gcttcctgca
     3061 acgctacacc gaaatgagcg tttcgtcgct ggaaaccatt gaatcgctgg agcaactgcg
     3121 cgtcctgtgg cacggctact cgattgccgt cgaaaccgcc aaagaagccc ccgccgccgg
     3181 tgtggatacg caggaagatt tggacagggt ccgcgccgta tttcagaccg tataaaacag
     3241 gttcaaaggg aaaagatatg cagcaacata ttgaaaagtg gcagcactta agccgtgaag
     3301 aacagaaaat ccttgccgaa gtatggggtc tcgtgcaaaa cgatgatcag gaggttcact
     3361 atgaaatgct taaattgaac gcacccgacg aagccagcgg cgaattttgg ttcagaatgg
     3421 cagaaacact cagcaccctg ccgcccaacc gttccctcga ccttagaatg aacggcggca
     3481 ggctggcgac cgccgtatcc atcctttccg tcatgattga agacaatccc gacataccgc
     3541 agctttgggc gcaaaaaatt accgcgctca attatagtgg attaaattta aaccagtacg
     3601 gcgttgcctc gccttgtcgt actatctgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     3661 ttttgttaat ccactatatt tggcacacgg gcacaaagcc cgtgccgacg gtttggcaca
     3721 acagcccgac aaagcggcag aagccaacga ggaggaatac ctgaccaaag ccctgtcgca
     3781 aaacctgctg tcaacattgg atgccgcgct tgcacgtttt cctgaagacg cgtggtttca
     3841 ggaaatcaaa caggatgcac aaaagcattt tgcttgagga tgtggcagtc aggaatattt
     3901 ccattcagga agaaaagaag tgcctgattg ggtataatca gggtaaatct tattttattt
     3961 caaaagatta atatttgctt tctgtttttc cttgacggta tcggaaaagt tgattatagt
     4021 tacagcttcc ttaggagtaa tggctgagag gctgaaggca cttccctgct aaggaagcat
     4081 gtggggtcaa cctgcatcga gggttcgaat ccctcttact ccgccagata aaaaatagac
     4141 gctgtgtttt acagcgtcta tttttttatg caattttata gcgggttggt gcaaaaccag
     4201 tatggtattg ccctgtcttg attctgaatt ttgttatagt ggattaacaa aaaccagtac
     4261 ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctg
//
LOCUS       DAWFAD010000146         4295 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWFAD010000146 DAWFAD010000000
VERSION     DAWFAD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.146"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(61..1317)
                     /locus_tag="WGO49_001745"
     CDS             complement(61..1317)
                     /locus_tag="WGO49_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ0306822.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPSDAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWNEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLQSLKIDL
                     DTGKYLRLDSAKHQTDGFFAAVLQRK"
     gene            complement(1353..2210)
                     /locus_tag="WGO49_001746"
     CDS             complement(1353..2210)
                     /locus_tag="WGO49_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ0306823.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQSFHAFEPPLNPYGWRGIYIQD
                     WAEYGFERQEVRYHLLDRMAYLAPARAAETETLNETEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(2271..3086)
                     /locus_tag="WGO49_001747"
     CDS             complement(2271..3086)
                     /locus_tag="WGO49_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0306824.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            3150..3320
                     /locus_tag="WGO49_001748"
     CDS             3150..3320
                     /locus_tag="WGO49_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306825.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            3330..4115
                     /locus_tag="WGO49_001749"
     CDS             3330..4115
                     /locus_tag="WGO49_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ0306826.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDIVAANPKVLAQMLKIISAHV"
BASE COUNT          883 a         1050 c         1304 g         1058 t
ORIGIN      
        1 tgatgaaatg ccgtctgaac gctttgaaaa gggttcagac ggcattttgt ttaaacaggg
       61 ttatttgcgt tgcaatacgg cggcgaagaa gccgtcggtt tggtgttttg ccgaatcgag
      121 gcgcaggtat ttgccggtat ccaaatcgat tttcaggctt tgcagaagtt cggcgcagtt
      181 gacgggttca aattcgggat gttcggacag gaaacgttcg acttgcagct cgttttcttc
      241 gggcaggatg ctgcaagtgg cgtaaaccaa gcggccttgc ggttttacca gtttggaggc
      301 agcatcgagg atgctgtgtt gctgttccaa aaggttggcc acggtctcgg cagattggcg
      361 gtatttgagg tcgggattgc ggcgtaaagt acccaaaccg gagcagggcg cgtccaccaa
      421 cacacggtcg gctttgcctg ccagtcgggc gatacggctg tcgtgttcgc tgctgatgcg
      481 ttcggggtgg atattggtca gtccggcgcg ggtcatacgc ggtttgaggt tggcaaggcg
      541 tttttcggcg atgtcgaagg cgtagattct gcctttattg gccatttgcg caccgacagc
      601 caaggttttg ccgccggcac cggcacagaa atcgacaatg atttcgccgc gttttgcgcc
      661 caccaataaa gcaagcagct ggctgccttc gtcttggact tccagtgtgc cgtctaaaaa
      721 cagttcgtgt ttgttaagcg cgattttgtt tttcaggcgg atgccccaag gcgaataagg
      781 cgttgcctct gcatcggcac tttcggcttg caacagcggc agcactttat cgcgtttgcc
      841 tttcaaagtg ttgacgcgga tgtcgagcgg cgcggcttgg ttgatgctgc gtccgaaggc
      901 gaggatttct tcttcgttcc aatgctgttg cagttgttcg accagccatt gcggcaattc
      961 tgcggcggta ttcagaccgt ctgaaaactc ggttttgcgg gctttcaaat tgccgaggaa
     1021 ctctgtttct tcttcatcaa gcaggtcttt gatttggctg atgttggtgc ttctgccgag
     1081 aaccagtgcg gcgagtgcgg ctttgcgtgg ctgcgcgtga ggacggcgca gggcagtgct
     1141 gattttttga tagtggcgca gcgcggcaaa ggcggtttcg gcgatttcgt ggcggtcttg
     1201 gcggccgagc tttttatgtt cgcggaagta ggcggagagg acggcatcgg aaggctgttt
     1261 gaaagtcagc atttcagcca aaactttggc ggtatggtcg agttgtgcgg cgttcataat
     1321 gtaatgggtt ctcttaaagt tggcaaaggg tttcagacgg ccttcataat gcgttcgatt
     1381 tcgtgccaaa agccgtccgg ccgacattcc aaaacggcaa tcaagccttg gtcgatacgg
     1441 cggatttcgg tttcgttcaa tgtttcggtt tcggcggcac gcgcaggtgc gaggtaggcc
     1501 atgcggtcga gcagatggta gcggacttct tggcgttcaa acccgtattc cgcccagtct
     1561 tgaatataga tgccgcgcca gccgtatggg ttaagcggcg gctcaaaagc atggaaactt
     1621 tgtggaaaaa atccgatgcc gcgcacgatg gaaacgggtt ttacgtcaag cggcagaccg
     1681 tgttgccgca aggttcggat gccgtctgaa gtgtgcggca gaccgagttg ggcggtcagt
     1741 ttggcggctt ttcccaacag cgtgtctttg gggtcgagtc cgcgcatgtc ttcgagcctg
     1801 cccgtgccgc cgccgtaata tttgcaggtc agctcgatat ggtagggctt gccgttaagc
     1861 cttaccacaa aatccgccgc gccttgcgta ttgccgtccg aaccggaaac cgtgaggttg
     1921 tgcgcgagca gttcggcgtg tggcgcgttg gcaaaccaaa atgccagcag ttcttcggcg
     1981 taaatgccca agcggtgtcc gaacggggcg cgtcgggtga ggtgttccgt cagcggcgcg
     2041 ggatcggcat ccaatgccaa aagatagcgg aaaccgcgtt cccccaatag ttcgcgcacg
     2101 cccaattcgc aaccgctttg ccacaaaggc ggcgcagtca gcagcgaggc gaggtcgcgg
     2161 acgggttggc tggtgagctt ccaccataat gcgtctaggg cgtaattcat gcttatccga
     2221 atatcagtca tgccgtctga aaagctggaa atgttttcag acggccttaa tcagtctttt
     2281 ttatggatac gatggctgac ggtattgaaa aaaccgcgca ggatatcgat gtcttcggtt
     2341 tgcgtgttgg cgcgtccgaa caggctctgc atacggcgca tcagacgctc gccgttgcgg
     2401 cggttgaaaa agccgatgtc gttcatcacg ctttccatgt gggcgaccat gcctttgatt
     2461 tgctcgtggg tcgcagcgtg gtcttcttgt tgaagatggg tcatgggcga accggtttgg
     2521 ctgaagattt cgtagcacac gacctgcacg gcttgggcga ggttgagcga gaaatagtcg
     2581 ggattgccgt tgatggtcat cagtcggttg caggcttgga cttcttcgat gctcaagccg
     2641 aaagtctcgt tgccgaaaac cagtgccact ttctcgcctc ggtttgcggt ctgcagtaat
     2701 tcggatacca aatcgcgcgg ggtttgcagc ggcgcagtaa tttcgcggcg gcggctggtc
     2761 agggcgcagg cgatggtggt gtcggcaagg gcttcgtcca aagaagcggc aatggtggca
     2821 ttttccaaaa cgtctgccgc gccggaagcg aggatgaagc tttcttccgg taatttaaac
     2881 gattgaggat gctccgggtc aaacacgggc gggttttccg tcatcggcgt tgccatcaga
     2941 tttggggcga cgatggtcag tttgtgcaga cccattgttt tcatcgcgcg cgcggcagag
     3001 ccgatgttgg cgggatggct ggtgcgcgtg aggatgatgc ggatgttgtc cagataagcg
     3061 ggcagggcgg gtttgagagt agtcatgttc ggttttcggt cggaatggaa tagggtataa
     3121 tgcgccgtct ttttcgttcg gacggtcaaa tgccgtctga aatgttcttt aacaacgtcg
     3181 gaaatttaac gttgcgcctg aagcagacac gcgtcgggcg gtattttacc cgatttggca
     3241 ggatttaccc caaccaaata aactccgatt ttgccgctcc gaaggactgc gcgtccgaac
     3301 cggcggtatg ccacccttaa ggaaatctga tgaatccgtt tttgaataca gcctttaaag
     3361 ccgcccgccg tgccggtcag atgatgatac gcgccgcggg caatctcgat gccgtcaaaa
     3421 ccgacagcaa agcattcaac gattttgttt ccgatgttga ccgcaattcc gaaatcatcc
     3481 tggttgaggc tttgaaagaa gcctatccgc accacaaaat cacttgcgaa gaaagcggct
     3541 cccacggtaa agccgcagcc gaatacgaat ggattatcga tccgctcgac ggcacgacca
     3601 acttccttca cggccatccc caatacgcca tctctatggc actcctgcac aaaggcgtgt
     3661 tgcaagaagc tttggtgtac gcccccgaac gcaacgacgt ttacatggct tcgcgcggca
     3721 aaggcgcgtt gctcaatgac cgccgcatcc gcgtttccaa ccgcatcgaa ttgaaccgct
     3781 gcctgatcgg taccggcttt cctgttgtcg atcaaagcat gatggacaag tatctggcga
     3841 ttttgaaaga tttcttggca aaaaccgccg gcggccgtcg tgaaggcgcg gcttctttgg
     3901 atttgtgtgc tgtggcgact ggtcgttttg acggcttttt cgagttcaac ctcaaacctt
     3961 gggatattgc cgcaggcgca ttgattgttc aggaagcggg cggtatcgta accgatatga
     4021 cgggtgaaga cggctggctg gaaagcggcg atattgttgc cgccaatccc aaagtgctgg
     4081 cgcaaatgtt aaaaatcatt tccgcacacg tttaaatttc agcccgctcc cgggaatgcg
     4141 ggaaaacggg cgggtatgcc gtctgaagta tcgcgccgat atttcagacg gcatttttac
     4201 tggcagcggg ggcaggaagg tggaaacggc taaatgcccg aaactgccga agatggaaac
     4261 ccatatctga ttaaacggag aaccctgtac gattt
//
LOCUS       DAWFAD010000147         4260 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWFAD010000147 DAWFAD010000000
VERSION     DAWFAD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.147"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            537..2264
                     /gene="ilvB"
                     /locus_tag="WGO49_001750"
     CDS             537..2264
                     /gene="ilvB"
                     /locus_tag="WGO49_001750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ0306827.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKVDIPIVGDVKNVLSEMVALW
                     QKQEFMPSEDALGKWWKTIEEWRSQDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            2275..2766
                     /gene="ilvN"
                     /locus_tag="WGO49_001751"
     CDS             2275..2766
                     /gene="ilvN"
                     /locus_tag="WGO49_001751"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ0306828.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            2833..3126
                     /locus_tag="WGO49_001752"
     CDS             2833..3126
                     /locus_tag="WGO49_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ0306829.1"
                     /translation="MSNIKIVALVTVKPEYTETLVAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            3206..4219
                     /gene="ilvC"
                     /locus_tag="WGO49_001753"
     CDS             3206..4219
                     /gene="ilvC"
                     /locus_tag="WGO49_001753"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ0306830.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVTANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
BASE COUNT         1105 a         1015 c         1077 g         1063 t
ORIGIN      
        1 tcaatatatt aagtcgttgt tagccctctt ggttctgtca atgccgtcca atacagtccg
       61 gtgcaatgag ccattttcag gaagtatggt tgggaatatt tttgatatat caaaaataaa
      121 atcaagttca atcaatatcc cgagtcttta ttgggatgaa accctgtaaa ttttacaatt
      181 cttgatatcg aagaagggtt tttcacatct gcccctccta cacaaactgt caacaatttc
      241 catctttcaa aaaaggtact cctgatttta ttcttttaaa atcaatcaat aatttctctg
      301 ctttcaaaaa atattaaaag atgaaagatt tttcttgatt gaactgtcgg tttgcgttat
      361 cgtttctact ctttttcaca aactctgtgt tcctttccaa ttgattggat aatccgtctg
      421 ttgccgtgtt cccccaatgc gcgtacccta aatcgctgct tggtggaatt gcattacagg
      481 tgctgtggca aagcggtttg tccctattgg tttgaaaccg tataaaagag gtcgttatgc
      541 agttatcagg cgcgcaaatc atagtgcaga gtctcaaagc cgaaggtgtc gagtacgttt
      601 tcggttatcc cggcggtgcg gttatcgaaa tctacgatgc ccttttccaa ctcaataaat
      661 tcaagcacat tctgacccgt cacgagcagg cggcagtaca cgcggcagat gcgtatgcgc
      721 gcgtcagcgg taaggtgggc gtggcattgg ttacatccgg cccgggcgtt accaatgcac
      781 tgaccggtat tgctactgcc tatacggatt cgattccgat ggtggtcatc agcgggcagg
      841 taggcaattc cctgattggt acggatgcgt tccaagaagt tgatacggtg ggtattaccc
      901 gtccgtgcgt caaacacaat ttcctggtta cggacatcaa tgagttggcg gaaaccatta
      961 aaaaggcgtt ccaaattgcc gcaagcggcc gaccggggcc cgtggtggtt gatgtcccga
     1021 aagatgttac gcaggcgatg gcgaaattca gctatcctca ggaagacatt tttatccgtt
     1081 cgtatcaacc ggttgttcaa gggcatatcg gtcagattaa aaaggccgtg cagatgttgg
     1141 catctgccaa acgtccggtc gtctattttg gcggcggcgt ggtgttgggt aatgcttctg
     1201 aagagctgac ccgatttgtc cgaatgacgg gtgctccgtg cacgggtacg ctgatgggct
     1261 tgggcgctta tccttccggc gaccgccaat tcctcggtat gctcggtatg cacggtactt
     1321 acgaggcaaa ccttgccatg cagaatgcgg atgtcgttct tgccgtaggt gcgcgttttg
     1381 acgaccgtgt ggtttccgta ccgtccaaat ttttcgagaa ggcgaagaag gttatccata
     1441 tcgatgtcga tccttccagc atcgccaaac gcgtgaaggt ggacattccg attgtcggcg
     1501 acgtgaaaaa tgttttgtct gagatggttg cgctgtggca aaaacaagag ttcatgccgt
     1561 ctgaagatgc tttgggcaaa tggtggaaaa ccatagagga atggcgttcc caagattgct
     1621 tgtggtttga caacggcagc gaaattatca agccacaata tgtgattcag aagcttgccg
     1681 agattaccgg caattcggca atcatcacat cggatgtagg gcagcatcaa atgtttgcgg
     1741 ctcaatatta tcccttcgaa cgtccgcgcc aatggctcaa ttccggcggt ttgggtacga
     1801 tgggcgtggg tctgccttat gcgattggtg caaaacttgc cgccccggat caagacgtat
     1861 tctgtattac cggcgacggc tcgatccaga tgaacatcca agagttgtcc acctgtttcc
     1921 aatatcggat tccggttaac gtcattacgc tgaacaacgg ttatctcggt atggtacgcc
     1981 agtggcagga aatatattac ggcggccgag agtcggaaac ttatttcgat tctttgcccg
     2041 atttcgtcaa acttgccgag gcatacggcc atatcggtat ccgcgtggac aagaagtctg
     2101 atgtggaagg tgcgttgttg gaagcattga accaaaaaga caggctggtg tttatcgact
     2161 tcctgaccga ccagaaacag aatgtgatgc ccatggtcgg caacggcaaa ggtttggacg
     2221 aaatggtact tccgccgcat atgcgtgcgg acgaaaaggc gtaaggagag gcaaatgcga
     2281 catatcttat ctgttctgat tgaaaacgaa tcaggtgcga tgagccgcgt ggtcggtttg
     2341 ttctctgcac gcgattacaa tatcgattct ttggcggttg cgccgaccga agacaaaacc
     2401 ctgtcccgta tgaccatcgt tacccacggc gacgagcagg ttatcgaaca aatcaccaag
     2461 caactcaata aattgattga agtgattaaa gtggtcgatt tgaacgaaag ccgttttgtc
     2521 gaacgcgaac tgatgttggt aaaagtccgt gccgccggca aagaccgcga cgaatttttg
     2581 cgtttgaccg aaatctaccg gggcagcatc atcgacgtaa ccgaccgcag ctatacgatt
     2641 gaaattaccg gctcgaccga caagctggat tcctttttgg aaacggtcgg acgcgcccaa
     2701 attttggaaa ccgtacgtac aggcgcggcc ggcatcggtc gcggtgagcg tattttgaaa
     2761 atttaacgcc gtaacctttc agacggcacg gtatttgaat gccgtctgaa aaacgaacgg
     2821 caggagagat ttatgtcgaa cattaaaatt gtcgcactgg ttaccgtcaa accggaatac
     2881 acagaaacac tggtagcaca gtttaaagaa ctggtcaaag ccagccgtgc agaagagggc
     2941 aacatcagct acgatctcca tcaggaaatc ggtaaaccga accgttttgt tttcgtggaa
     3001 aattggaaat cccaagcagc tattgacgaa cataacgcca gcgcgcattt ccaagccttc
     3061 gtccagtccg tcgacggcaa aaccgaagcg ttggaaatcg tattgatgaa tgaagttgcc
     3121 gcttaagcgg cacactctgt ttaacccgtc tgaagccgct caacatttta aggcttcgac
     3181 aaccatttac cttaaaggaa atcaaatgca agtctattac gataaagatg ccgatctgtc
     3241 cctaatcaaa ggcaaaaccg ttgccatcat cggttacggt tcgcaaggtc atgcccatgc
     3301 tgccaacctg aaagattcgg gtgtaaacgt ggtgatcggt ctgcgccaag gttcttcttg
     3361 gaaaaaagcc gaagcagccg gccatgtcgt caaaaccgtt gctgaagcga ccaaagaagc
     3421 cgatgtcgtt atgctgctgc tgcctgacga aaccatgcct gccgtctatc acgccgaagt
     3481 tacagccaat ttgaaagaag gcgcaacgct ggcatttgca cacggcttca acgtgcacta
     3541 caaccaaatc gttccgcgtg ccgacttgga cgtgattatg gttgccccca aaggtccggg
     3601 tcataccgta cgcagtgaat acaaacgcgg cggcggcgtg ccttctctga ttgccgttta
     3661 ccaagacaat tccggcaaag ccaaagacat cgccctgtct tatgcggctg ccaacggcgg
     3721 caccaaaggc ggtgtgattg aaaccacttt ccgcgaagaa accgaaaccg atctgtttgg
     3781 cgaacaagcc gtattgtgcg gcggcgtggt cgagttgatc aaggcgggtt ttgaaaccct
     3841 gaccgaagcc ggttacgcgc ctgaaatggc ttacttcgaa tgtctgcacg aaatgaaact
     3901 gattgttgac ctgattttcg aaggcggtat tgccaatatg aactactcca tttccaacaa
     3961 tgcggagtac ggcgaatacg ttaccggccc tgaagtggtc aatgcttcca gcaaagaagc
     4021 catgcgcaat gccctgaaac gcattcaaac cggcgaatac gcaaaaatgt ttatccaaga
     4081 gggtaatgtc aactacgcgt ctatgactgc ccgccgccgt ctgaatgccg accaccaagt
     4141 tgaaaaagtc ggcgcacaac tgcgtgccat gatgccttgg attactgcca acaaattggt
     4201 tgaccaagac aaaaactgat tgttttcaaa cgggactgcc tacacatcgt gtaggcagtt
//
LOCUS       DAWFAD010000148         4234 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWFAD010000148 DAWFAD010000000
VERSION     DAWFAD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.148"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            13..1326
                     /locus_tag="WGO49_001754"
     CDS             13..1326
                     /locus_tag="WGO49_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ0306831.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARTLAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRAPTAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHQHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            1409..2128
                     /locus_tag="WGO49_001755"
     CDS             1409..2128
                     /locus_tag="WGO49_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0306832.1"
                     /translation="MATLSDKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGYPEPFAICFDLISAEEKEFEHFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            2293..2964
                     /locus_tag="WGO49_001756"
     CDS             2293..2964
                     /locus_tag="WGO49_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ0306833.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            2974..3342
                     /locus_tag="WGO49_001757"
     CDS             2974..3342
                     /locus_tag="WGO49_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ0306834.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            3339..3986
                     /locus_tag="WGO49_001758"
     CDS             3339..3986
                     /locus_tag="WGO49_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ0306835.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
BASE COUNT          996 a         1300 c         1109 g          829 t
ORIGIN      
        1 ttggaggaat aaatgatgaa cacgccgcat ccgcgcccga aaatcgccgt catcggcgca
       61 ggctgggcag gactgtccgc cgccgtcacc ttggcgcggc acgccgacgt taccctgttt
      121 gaagccggcc ggcaggcggg cggcagggcg cgcacactgg ccggaaatac cgacggtttc
      181 ggttttttgg acaacgggca gcacattttg ctcggcgcat accggggcgt gttgcgcctg
      241 atgaaaacca tcggttcgga tccccgtgcc gcctttttgc gcgtaccgct gcactggcat
      301 atgcacggcg gtttgcagtt ccgcgccctc cccctgcccg cgccgctgca tattttgggc
      361 ggcgtgctgc ttgcccggcg tgcaccgact gcattcaaag ccaaactgct tgccgatatg
      421 tccgatttgc agaagtccgc acgcctcgga cagcccgaca cgacagtggc gcaatggctg
      481 aaacagcgga acgtgccgcg tgccgccgtg atgcagtttt ggcagccctt ggtttggggc
      541 gcgctcaaca cgcctttgga aaccgcaagc ctgcgcgtgt tgtgcaacgt tttgtccgac
      601 ggcgtgctga cgaaaaaatc cggcagcgac tatctcctac ccaagcagga tttgggcgca
      661 atcgtcgccg aacccgcctt ggcggagctt caacggctcg gcgcggacat ccgcctcgaa
      721 acgcgcgtat gccgtctgaa caccctcccg gacgggaaag tgctcgtcaa cggcgaagct
      781 ttcgatgccg ccgtccccgc caccgcgccc taccacgccg ccgcgctcct gcccgaaggc
      841 acgcccgaac acgttcagac tgcatatcaa aaccttcgct accacgccat caccaccgtc
      901 tatctgcgct acgccgaacc cgtccgcctg cccgccccgc tgaccggcct tgccgacggc
      961 acggtgcaat ggctgctttg ccggggcagg ctcggactgc ctgaaaacga agtgtccgcc
     1021 gtcatcagcg tttccgaccg cgtcggcgcg tttgcaaacc gggcgtgggc ggacaaagtt
     1081 cacgccgacc tcaaacgcat ccttccgcat ttgggcgaac ccgaagccgt gcgcgtcatc
     1141 accgaaaaac gcgccacaac cgcagccgat gccccgccgc cggatttgtc gtggttgcac
     1201 cagcaccgca tcttccccgc cggcgactac ctccacccag actaccccgc cacgctcgaa
     1261 gccgccgtac aatcaggttt cgcgtcggcg gaagcctgcc tgcaaagcct gagcgatgcc
     1321 gtctgaaaac gccggccggt atcgggacgc tttacaaggt gcggcaaaac gctaaaatac
     1381 cttaaccgac caccaaaaag gaaaaagtat ggcgacactg tccgacaaaa ccatcttagt
     1441 taccggcgca tcgcaaggtc tgggcgaaca ggtcgccaaa gcctatgcgg cggcaggcgc
     1501 aaccgtgatt ctggtggcac gccatcagaa aaaactggaa aaagtctatg acgcgattgt
     1561 cgaagccgga taccccgaac cattcgccat ctgctttgac cttattagcg cggaagaaaa
     1621 agaatttgaa catttcgccg ccaccattgc cgaagccacg caaggcaaac tggacggcat
     1681 cgtccactgc gccggctatt tttacgccct ctcgccgctg gatttccaaa ccgtcgccga
     1741 atgggtcaac caataccgca tcaacaccgt cgcacctatg gggctgaccc gcgccctgtt
     1801 cccgctgctg aagcagtcgc ccgacgcgtc cgtcatcttc gtcggcgaaa gccacggcga
     1861 aacacccaaa gcctactggg gcggcttcgg cgcgtccaaa gccgcgttga actacctgtg
     1921 caaagtcgcc gccgacgaat gggaacgctt cggcaacctg cgcgccaacg tcctcgtccc
     1981 cggacccatc aattccccgc aacgcatcaa atcccatccg ggcgaagcca aaagcgaacg
     2041 caaaagctac ggggacgtgc tgcccgcatt tgtctggtgg gcaagtaccg aaagcaaagg
     2101 gcggagcggc gaaatcgttt acctctaaat ccggaaaagg caggcatccc gcccgcgccg
     2161 cgattccgtt tgaaaaatat ccgtaccttc cgcgcgcatc cgtcccgcac agacaaacac
     2221 cttgacttaa atttacataa ggacaataat gataacccgt tatttcctta acttgacctt
     2281 agggtaaatt ttatgaaaac cgtttccacc gccgttgtcc ttgccgccgc tgccgtttca
     2341 ctgaccggct gtgcgaccga atcctcacgc agtctcgagg tagagaaagt cgcctcctac
     2401 aatacgcaat atcacggtgt tcgtaccccg atttccgtcg gaacattcga caaccgctcc
     2461 agcttccaaa aaggcatttt ctccgacggg gaagaccgtt tgggcagcca ggcaaaaacc
     2521 attctagtaa cgcacctgca acagaccaac cgcttcaacg tactgaaccg caccaatttg
     2581 aacgcattaa aacaggaatc cggcatttcc ggcaaagcgc ataacctgaa aggcgcagat
     2641 tatgtcgtta ccggcgatgt aaccgaattc ggacgcagag atgtcggcga tcatcagctc
     2701 ttcggcattt tgggtcgcgg caaatcgcaa atcgcctatg caaaagtggc tctgaatatc
     2761 gtcaacgtca atacttccga aatcgtctat tccgcacagg gcgcgggcga atacgcactt
     2821 tccaaccgtg aaatcatcgg tttcggcggc acttccggct acgatgcgac tttgaacggc
     2881 aaagttttag acttggcaat ccgcgaagcc gtcaacagcc tggttcaggc tgttgacaac
     2941 ggcgcatggc aacccaaccg ttaaggagaa atgatgaatc cgaaaacctt gagccgtttg
     3001 tcgctgtgtg cggcggtctt ggctctgacc gcctgcggcg gcaacgggca aaaatccctg
     3061 tattattacg gcggctatcc cgataccgtc tatgaaggtt tgaaaaacga cgacacttcg
     3121 ttgggcaagc agaccgaaaa gatggaaaaa tactttgtgg aagccggcaa caaaaaaatg
     3181 aatgccgccc cgggtgcgca cgcccatctg ggactgctgc tttcccgttc gggagacaaa
     3241 gagggcgcgt tccgccagtt tgaagaagag aaaaggctgt ttcccgaatc gggcgtattt
     3301 atggacttcc tgatgaaaac aggtaaagga ggcaagcgat gaaacctttg attttagggc
     3361 ttgccgccgt gttggcgctg tctgcctgcc aagttcaaaa agcgcccgat ttcgactaca
     3421 cgtcattcaa ggaaagcaaa ccggcttcaa ttttggtggt tccgcctctg aacgaatcgc
     3481 ccgatgtcaa cggaacatgg ggtgtactgg cttcgaccgc cgcgccgctt tccgaagccg
     3541 gctattacgt cttccccgcc gcagtcgtgg aggaaacctt caaacaaaac ggcttgacca
     3601 atgccgccga tattcacgcc gtccggccgg aaaaactgca tcagattttc ggcaatgatg
     3661 cggttttgta cattacggtt accgaatacg gcacttcata tcaaatttta gacagcgtga
     3721 cgaccgtatc cgccaaagca cggctggtcg attcccgcaa cggaaaagag ttgtggtcgg
     3781 gttcggccag catccgcgaa ggcagcaaca acagcaacag cggcctgttg ggggctttgg
     3841 tcagcgcagt ggtcaatcag attgccaaca gcctgaccga ccgcggttat caggtttcca
     3901 aaaccgccgc atacaacctg ctgtcgccct attctcacaa cggcatcttg aaaggtccga
     3961 gattcgtcga agagcagccc aaataagcat atgccgtctg aacctctccg agcttcagac
     4021 ggcatatgcg aatcttgata cttgcaagcc gcaccgccca aaatcgagcg gccggtttca
     4081 atatccgaac aaggttcggg ttctgtattt tgcagaaccc gaacctttta ttcaaattta
     4141 aacttaattc cggcgtggtt gtatggtata gtggattaac aaaaatcagg acaaggcgac
     4201 gaagccgcag acagtacaaa tagtacggaa ccga
//
LOCUS       DAWFAD010000149         4232 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWFAD010000149 DAWFAD010000000
VERSION     DAWFAD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.149"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..565
                     /locus_tag="WGO49_001759"
     CDS             <1..565
                     /locus_tag="WGO49_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0306836.1"
                     /translation="FRKTKKECAVLGTLSRLDTVRAVHLMLDILKKMVDRGMPVRLNV
                     AGIGEEMDNLKAQAKRLGIEDKVTFLGGVRDLTGYFKEVDILVNTPHCVGDHGAGVGN
                     NILEAGLYDTPVVTYNMAGISEMVITGQTGYCIPFGDDEAFIEAVDTLIKHPELRSQM
                     GKALHKHVETLCSDDEIYRTTMAAYEM"
     gene            579..1751
                     /locus_tag="WGO49_001760"
     CDS             579..1751
                     /locus_tag="WGO49_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0306837.1"
                     /translation="MNITIVAPYCSLPSEPHFNRFWYLAELLSQSHDVLLITSNFKHY
                     DKSFRRPEDAEAASQGRLKVMLLEESGYSKNVSLGRVTSHHRFVKHFEKWLENCRPGE
                     QDVVFSAYPLIATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHNLLPFA
                     SRANRAYRYADALVAVSQTYLDRAKETNPNVPGEVIYIGADFPKLDAAPAKDFGDDKT
                     RFFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLDRLKQYACDGIKFYGY
                     IPYAEMMSVAKGCDISVNAIHSHAMQSVTNKLSDYMALQKPILNSQVNDEVAEVLTLL
                     PHADYRSGDVDGFVQADKDILKRKNDPVQSEEIVRRFKRDVAYQKIVNLIERLAHE"
     gene            1744..2361
                     /locus_tag="WGO49_001761"
     CDS             1744..2361
                     /locus_tag="WGO49_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046551259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEZ0306838.1"
                     /translation="MNKFFKRLFDIIASASGLIILSPVFLILVYLIRKNLGSPVFFIQ
                     ERPGKDGRPFKMIKFRSMRDAIDKDGNPLPDSERLTDFGKKLRATSLDELPELWNVLK
                     GEMSLVGPRPLLMGYLPLYNEFQNRRHEMKPGVTGWAQVNGRNAISWDEKFAHDVWYI
                     DNFSFWLDIKILFLTVKKVFVKEGISAEGEATMPYFTGNKTDEKK"
     gene            2348..3322
                     /locus_tag="WGO49_001762"
     CDS             2348..3322
                     /locus_tag="WGO49_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ0306839.1"
                     /translation="MKKNNILILSAGRRVELVQDFQTEAARFSDGISVFATDLNPRMS
                     SACHVSDGSFEVPPISADSYIDSIFNLAVRENIGLIIPTIDTELQKLADERERFEAAS
                     VHIVVSDSDFIAQCRDKRKTATLFARYGIRSPEIYDRKKLVFPCFAKPYDGSRAIGAK
                     RIDTPADLTPEVLADTKLMFCQFIDIENKFSEFTVDMYYDRQCRLKCAIPRKRLEVRS
                     GEISKGVTCKNSLYQTLLEKMSVLQGARGCITAQFFYNEETGEFYGVEINPRFGGGFP
                     LTYAAGGNYPSRLMREYLGDEEIPFSDDWENNLIMLRYDAKVLVHEND"
     gene            3309..3995
                     /locus_tag="WGO49_001763"
     CDS             3309..3995
                     /locus_tag="WGO49_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0306840.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDSKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSFQ
                     DLVPIIDSNT"
BASE COUNT         1083 a         1180 c          996 g          973 t
ORIGIN      
        1 tttccggaaa accaaaaaag aatgcgccgt actcggcaca ctttcccgtt tggacaccgt
       61 ccgcgccgtg catctgatgt tggatatttt gaagaaaatg gttgaccgcg gtatgcctgt
      121 acgcctcaac gttgcaggta tcggcgaaga aatggacaac ctcaaagccc aagccaaacg
      181 tttgggcatt gaagacaaag tcaccttcct cggcggcgtc cgcgatttga ccggctactt
      241 caaagaagtc gatattttgg tgaacacgcc gcattgcgtg ggcgatcacg gcgcaggtgt
      301 cggcaacaat attttggaag ccggccttta cgacacgcct gtcgtgacct acaacatggc
      361 gggcatttcc gaaatggtca ttaccggcca aaccggctac tgcattcctt tcggcgacga
      421 cgaagcattt atcgaagccg tcgatacact catcaagcat ccagagttgc gcagccaaat
      481 gggcaaagcc ctgcacaaac atgtcgaaac cttatgctcc gacgacgaaa tctaccgcac
      541 caccatggct gcgtacgaaa tgtaaggccg tctgaaacat gaacattacc atcgtcgccc
      601 cttattgctc gctgccgtcc gagccgcatt tcaaccgctt ctggtatctt gccgagctgt
      661 tgtcgcagtc gcatgacgtg ttgctgatta ccagcaactt caagcactac gacaaatctt
      721 tcagacggcc cgaagatgcc gaggccgcct cacaaggccg tctgaaagtc atgctgttgg
      781 aagaaagcgg atacagcaaa aacgtgtctt taggacgcgt gaccagccat caccgcttcg
      841 tcaaacattt tgaaaaatgg ttggaaaact gccgtccggg cgaacaagac gtcgtctttt
      901 ccgcctatcc gctgattgcc accaacctac tgttgggcaa acacaaagcg cgtttgggtt
      961 acaaactgat tgtcgatgtg caggacgtat ggccggagtc tttctcctcg gtcgtaccgt
     1021 ttttgaaaaa agtaccgcac aacctgctgc cctttgcttc acgcgccaac cgggcctacc
     1081 gctacgccga cgcgctggtt gccgtatcgc agacttatct cgaccgcgcc aaagaaacca
     1141 atccgaacgt acctggcgaa gtcatctata ttggtgctga ctttcccaaa ctcgatgccg
     1201 cacctgccaa agattttggc gacgacaaaa cccgcttttt ctacttgggc acgctcagtt
     1261 acagctatga cgtggaaacc gtgtgcaaag gcgttcggaa acttttggac gacggcgaaa
     1321 atgtagagct gcacatcatg ggcggcggcc ccgatttgga caggctcaaa caatatgcct
     1381 gcgacggcat caagttttac ggctacattc cttacgccga aatgatgtcg gtcgccaagg
     1441 gctgcgacat ttccgtcaac gccatccata gccacgccat gcagtcggtt accaacaaac
     1501 tctctgacta tatggctttg caaaaaccga ttctgaacag ccaggttaac gacgaagttg
     1561 ccgaagtcct taccctgctg ccgcatgcgg actaccgctc cggcgatgtg gacggtttcg
     1621 ttcaagccga taaagatatt ttgaagcgca aaaacgatcc tgttcagtcc gaagaaatcg
     1681 tccgccgctt caaacgcgac gttgcctatc aaaaaatcgt caacctgatt gaaagattag
     1741 ctcatgaata aatttttcaa acgcctgttt gacattattg cctccgcctc agggctaatt
     1801 atcctgtcgc ctgtgttttt gattttggtg tacctcatcc gcaaaaactt aggctcgccc
     1861 gtgttcttca tccaagagcg gccgggcaag gatggcaggc cgtttaaaat gattaaattc
     1921 cgctccatgc gcgatgcaat cgataaagac ggcaacccgc tgccggacag cgaacgcctg
     1981 actgatttcg gcaaaaaatt acgcgccacc agtttggacg aacttcctga attatggaat
     2041 gtcttaaaag gcgagatgag cctggtcggc cctcgtccgc ttttgatggg ctacctgccg
     2101 ctttacaacg aatttcaaaa ccgccgccac gaaatgaaac cgggcgtgac cggttgggcg
     2161 caagtcaatg gccgcaacgc catttcatgg gatgaaaaat tcgcccacga tgtttggtac
     2221 atcgacaact tcagcttttg gctggatatc aaaattttgt tcctgacggt taaaaaagtc
     2281 ttcgtcaaag aaggcatctc agccgaagga gaagccacca tgccttattt cacgggtaat
     2341 aaaactgatg aaaaaaaata atatactgat tctgtcggca ggtcgccgtg tcgaattggt
     2401 gcaggacttc caaaccgaag ccgcacgttt ttcagacggc atcagcgttt ttgccactga
     2461 cctcaacccc cgcatgtctt ctgcctgcca tgtttcggac ggttcatttg aagtgccgcc
     2521 gattagtgcc gattcatata tcgacagcat tttcaatctg gcggtacgcg aaaatatcgg
     2581 tttaatcatc ccaaccatag acaccgaact gcaaaagctg gcagacgagc gcgaacgctt
     2641 tgaagcggca agtgtccaca tcgtcgtttc cgattcggac ttcatcgcac aatgccgcga
     2701 caaacggaaa accgccacct tgtttgcccg ctacggcata cgctcacccg aaatttacga
     2761 ccgcaagaag ttggttttcc cttgcttcgc caagccttat gacggcagcc gcgccatcgg
     2821 tgcaaaacgg attgacacac ctgccgacct gacaccggaa gtcttggcag acaccaaatt
     2881 aatgttctgt cagtttatcg acatcgaaaa taaattttcc gagtttaccg tcgatatgta
     2941 ttacgaccgt caatgccgtc tgaaatgcgc catcccgcga aaacgcttgg aagtgcgctc
     3001 cggcgaaatc agcaaaggcg ttacctgcaa aaacagcctc taccaaacgc tgctggaaaa
     3061 aatgtcggta ttgcagggcg cgcgcggctg tatcacggcg cagttcttct acaacgaaga
     3121 aaccggcgaa ttctacggcg tggaaatcaa cccccgcttc ggcggtggct tcccgctgac
     3181 gtatgccgca ggcggcaatt atccgagccg gttgatgcgc gaatacctcg gcgacgaaga
     3241 aatcccgttt tcagacgact gggaaaacaa cctcattatg ttgcgctacg atgccaaggt
     3301 cttggttcat gaaaacgatt aatcctgaaa ccaccgtcat cgtcttcgac ttggacgata
     3361 ccctgtattc ggaatacgaa tacaagctct ccggtatccg ttcggtcatc gataccgttg
     3421 ccgcactgta tcccgattgg aattcagaca acctatggcg caacatcgac cccgacggca
     3481 aagattggct ggacaaattg tgccgccact gtggttttaa tgaatcggaa aaacaggttc
     3541 ttttgtggca ataccggctg caccgcccga cgctgactcc gtatgctccg ccggatttcc
     3601 tgtccgaact gaccgcgccc ttcgccgcac gcgcgctgat taccgacggc agaagcctga
     3661 cccaacgcct gaagctggaa gccttgggtc tgtcttccct gtttgacgac atcctcatct
     3721 ctgaagcctg ctcttcggaa aagcccgaca gcaaacgctt ccgccatctt caagacaaat
     3781 atgccgacaa ggcaggctgc ttcatctata tcggcgacaa catttccaaa gactttatcg
     3841 cgccgaacac tttgggctgg ataaccatag gattgcttcc ctccggacag aacatccacc
     3901 gccaccagcc tgaaagtttt aacacagaat atcatcccga cttttgggtt aactcctttc
     3961 aagatttagt tcctataatt gattcaaata cttaaccatt acataatcaa acatgcactc
     4021 acaatattaa aaggactatt tcatgacaaa atattattca tggggcgtaa acggctttcc
     4081 gccctacgtt ttcgacacca tttggaaacg atggaagttg aatgggcgcg ggggcggtta
     4141 tcgtgtgcga catcccggac ggcatgaccg tcgcgggcaa tccggcaaag ccgctgccgc
     4201 gcaaaaaccc caagaccggg acggcataag cg
//
LOCUS       DAWFAD010000150         4216 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWFAD010000150 DAWFAD010000000
VERSION     DAWFAD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.150"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(83..1129)
                     /gene="recA"
                     /locus_tag="WGO49_001764"
     CDS             complement(83..1129)
                     /gene="recA"
                     /locus_tag="WGO49_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ0306841.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGIEMHIT
                     EGTQDETDGERPEE"
     gene            1386..2150
                     /locus_tag="WGO49_001765"
     CDS             1386..2150
                     /locus_tag="WGO49_001765"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ0306842.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEILHHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFKLL
                     DALIESRLPDIIDIELFSGETAVRRAVANAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLKAKELAAKPIVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            2170..4185
                     /gene="rep"
                     /locus_tag="WGO49_001766"
     CDS             2170..4185
                     /gene="rep"
                     /locus_tag="WGO49_001766"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ0306843.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVSDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDSMKKGNPSD"
BASE COUNT         1000 a         1153 c         1155 g          908 t
ORIGIN      
        1 aaactcaaat caagccgttc ggaggctgct caaaaaaaac ggtacttcgc aacagaagta
       61 ccgtttatcg ggatttcagg ttttattctt cggggcgttc gccgtcggtt tcgtcctgcg
      121 tcccttcggt gatgtgcatt tctatgccgt tgagggcgcg gatttttgcg tcgatttcgt
      181 cggcaatttc gggattttcc ttcagccaga cgcggacgtt gtctttgccc tgaccgattt
      241 tcgcgccgtt gtagctgtac cacgcgccgg atttgttgat gatgtcgttt ttcacgccga
      301 tgtcgatcaa ttcgccttcc caactgatgc cttctccgta gaggatgtca aactctgcct
      361 gacggaacgg cggcgcgact ttgtttttga tgactttgac gcgggtttcg ttgcccaata
      421 cttcttcacc ttttttgatg gatccggtgc ggcggatgtc gaggcggacg gaagaataga
      481 atttcagcgc gttgccgccg gtcgtggtct cggggctgcc gaacattacg ccgatcttca
      541 tccggatttg gttgatgaac acaaccagcg tgttggtttt tttgatgtgt ccggtcagtt
      601 tgcgcaaagc ctggctcatc aggcgcgcct gcagtccgac atggctgtcc cccatatcgc
      661 cttcgatttc ggctttgggg acgagtgcgg ctacggaatc gacgactacc atatctatgc
      721 cgcccgaacg gacgagtgtg tcgcagattt ccaaagcctg ttcgccggta tcgggctggg
      781 acaggtaaag ctcttcgact tttacgccga gtttgcgggc gtaaacggga tcgaaggcgt
      841 gttcggcatc gacaaaggcg cacacgccgc cgtttttctg gcattgggcg acggcttcga
      901 ggcagagggt ggttttgccg gaggattctg ggccgaagat ttcgacgatg cgcccgcgcg
      961 gcagaccgcc gactccgagg gcgaggtcta atccgagcga tccggtggaa atgacttcga
     1021 ggttttcttc ctgctggctg ccgtccattt tcatgatggc gcctttgccg aaactttttt
     1081 cgatttgcgc cagtgcggcg gcaagtgctt tgcttttgtc gtctgacatt ggggttactc
     1141 cggaacaaat gcggtatgtg ggatgcggcg caacacgggc tgcggcgcgg ggtgcgtatc
     1201 gttttcccga tgtgcgggct gtcggtaatg ccgctgcttc acggggctgc cattatcgca
     1261 tatttccttg cttgccgata tgcgccagga cgcggcggct tgtgccggaa cggtgtccgg
     1321 atgccgtcta aaaggcggcc ggctttgtta taatggcggc tgttttttct gtgtgtgcct
     1381 gttttatgtg ttcctgcctt gttgtcaaaa ataccgttat cggaagcgga cgcaccaaaa
     1441 tcgccgtgcc gcttgtcgcc cgcgatgccg ccgaactttc cgccgtactt gagcaaatca
     1501 aaaatatgcc cttcgatatt gcggagttcc gcgccgactt tttggaatgc gcgggcagta
     1561 tcggcgaaat attgcaccac acgcagaccg tccgcgacgc gctgcccgac aagccgctgc
     1621 tgtttacgtt cagacggcat ggcgaaggcg gctcgttccc gtgttcggac gattattatt
     1681 ttaaactgct cgacgcgctg atcgaaagtc gcctgcccga cattatcgat atcgagctgt
     1741 tttccggcga aaccgccgtc cggcgcgctg tggcgaatgc tcaaaaaaac ggcatcgccg
     1801 ccctgctctg caatcatgag tttcaccgca cgccgccgca agaagaaatc gtatgccgtc
     1861 tgaaacagat ggaggactgc ggcgcggaca tctgcaaaat tgcggtgatg ccgcaaagcg
     1921 cggaagatgt gctgactttg ctttccgcca cgctcaaagc gaaagagctt gccgccaaac
     1981 cgattgttac gatgtcgatg gggcagacgg gggcggtcag ccggcttgcc ggacaggtgt
     2041 tcggctcaag catcacgttc ggttcgggaa cgcaaaactc cgcgccgggg caaatcggcg
     2101 tatccgccct ccgtgcgaca ctcgactgcc tcgaaaacgg cgcagactga tttcagacag
     2161 catcaaaaca tgatgaaact caatccccaa cagctcgaag ccgtccgcta cctcggcggc
     2221 ccactgctcg tccttgccgg tgcaggcagc ggcaaaaccg gcgtgattac tcaaaaaatc
     2281 aagtatttga ttgtcaatgt cggctatctg ccgcataccg ttgccgcgat tacctttacc
     2341 aataaagccg ccgcggaaat gcaggagcgc gtcgccaaaa tgctgcacaa atcgcaaacg
     2401 cgcgggctga cgatttgcac gttccactct ttgggcatga agattctgcg cgaagaggcg
     2461 aaccatatcg gttacaaaaa aaacttctcc attctcgatt ctaccgacag cgcgaaaatc
     2521 atcggcgaac tcttaggcgg tacgggcaaa gaagccgtat tcaaggcgca gcaccagatt
     2581 tccttgtgga aaaacgattt aaaaacgcct gaagatgtcg ttcagacggc atcgaacatt
     2641 tgggaacaac aaaccgcacg cgtgtatgcg agctatcagg aaaccttaca aagctatcag
     2701 gcagtggact tcgacgactt gatccgcctg cctgccgtgc tgttgcagca aaacagcgaa
     2761 gtgcgcaaca aatggcagcg gcggctgcgt tatctgctgg ttgacgaatg ccaagatacg
     2821 aatacctgcc aatttacgtt gatgaaactt ctgaccggcg cagaaggtat gtttaccgcc
     2881 gtcggcgacg acgaccagtc catctacgca tggcgcggcg cgaatatgga aaacctgcgc
     2941 aaaatgcagg aaaactatcc gcagatgaag gtcatcaaac tggagcaaaa ctaccgctcc
     3001 accgcgcgga ttctcaaaat cgccaacaaa gtcatcgaga acaaccccaa gctgttcacc
     3061 aaaaaacttt ggtcgaaatt gggcgaaggc gagcccgtca aagtcgttgc ctgccaaaac
     3121 gagcagcacg aagccgactg ggtcgtcagc caaatcgtca aacaaaaact catcggcggc
     3181 gacaaaaccc aatatgccga tttcgccgtg ttgtaccgcg gaaagcatca ggcgaggatt
     3241 ttcgaggaag cattgcgcgg cgcgcgcatc ccctaccgtc tctccggcgg acaaagcttt
     3301 ttcgacaaag ccgaaatcaa agacgtgttg tcttatgtgc ggctgcttgc caaccccaat
     3361 gacgatcccg cctttctgcg tgccgttacc acgcccaaac gcggcatcgg cgatgtcacg
     3421 ctgggcaagc tcaacactta cgcgcacgaa cacgaatgca gcctgtatga agccgcacaa
     3481 aacgaagaag cccttgccac gctgaacaat accaaccgcc aacacctgca agcctttatg
     3541 gatatgttcg gcagctaccg cgccaaagcg gaaaccggcg aagcaggcga gttcatcaac
     3601 agcctgctcg aagaaatcga ctatgaaaac catttgatgc aaaacgaaga aggcaaggcc
     3661 ggcgaaatca aatggcgcaa tgtcagcgat ttggtatcat ggtttgcgcg aaaaggcggg
     3721 gaagacggca aaaacatcat cgaactcgcc caaaccgtcg ccttgatgac gcttttggaa
     3781 ggaaaagacg aagaagaaac cgatgccgtc tcgctatcca cgctacacgc cgccaaaggt
     3841 ttggagtatc cgtatgtttt ccttgtcggt tgcgaagaag gcgttttgcc gcacaacgac
     3901 agtatcgaag agggcaacgt cgaagaagaa cgccgcctga tgtacgtcgg catcacccgc
     3961 gccaaacgcc aactcacgct gacccactgc gtcaaacgca aaaaacaagg cacatggcag
     4021 ttccccgaac ccagccgatt catagacgaa atgccgcagg aagatttgaa aatcctgggg
     4081 cgcaaaggcg gcgagccgat tgtcagcaag gaagaaggca gacgcaacct tgccgatata
     4141 atcggaaggc tcgacagcat gaaaaaaggc aatccgtcgg attaaaccaa gatagggaaa
     4201 cgaagccgga gacaat
//
LOCUS       DAWFAD010000151         4192 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWFAD010000151 DAWFAD010000000
VERSION     DAWFAD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.151"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(79..315)
                     /locus_tag="WGO49_001767"
                     /pseudo
     CDS             complement(79..315)
                     /locus_tag="WGO49_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
     gene            511..969
                     /locus_tag="WGO49_001768"
                     /pseudo
     CDS             511..969
                     /locus_tag="WGO49_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            869..1237
                     /locus_tag="WGO49_001769"
     CDS             869..1237
                     /locus_tag="WGO49_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ0306844.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1306..2844
                     /gene="lnt"
                     /locus_tag="WGO49_001770"
     CDS             1306..2844
                     /gene="lnt"
                     /locus_tag="WGO49_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ0306845.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3108..3971
                     /gene="rpoH"
                     /locus_tag="WGO49_001771"
     CDS             3108..3971
                     /gene="rpoH"
                     /locus_tag="WGO49_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ0306846.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
BASE COUNT         1020 a         1295 c          967 g          910 t
ORIGIN      
        1 cgaccttgcc gtcgggcagg atgtaccacg ccaaatcccc gcctgaagca cggtgtcgga
       61 ctgcatttcg attcggctat acgccttttc acgcaggctg taattgaatt tccacaaacc
      121 gccgaacact gccggcggac gcgcgaccag catcaggaac gccgcaatcg ccaaggcttc
      181 cgccgccccg tccaacacgc cggcgggaga aaccagcagc aaaggcattc ccaaacgtgc
      241 gggtaaagtg gtcgaaacca cgctcagaaa caacagtgcg ccacccggca ggaacagact
      301 gcccatccaa tccacgctaa cgattctccc ttaaaattca ttaaattaaa atcgaaagac
      361 tgtaacacgg cattttcttt tcacgaaact ttacttcacg tccgccacag cgttcagacg
      421 gcattttcct gtttcatgcc gcctgaaacc gcccaaatcc gcgttctcct atataatgtc
      481 ggactttgcg ccgcatttca ggattcttta atggcatttg cctcactatt taccctgccg
      541 gacgacatca ccgccgtttt ggacgatgtt gccctgatga ccaaagccgc cgcaaagaaa
      601 accgccggcg tggtcggcga cgatttggca cccaatgcca accgtgttac gggcgtatcg
      661 gcagaacgcg agttgccgat tgtttggtcg atggcgaaag gttctttttc caacaagccg
      721 gttttggtgc ctgcggccct gctgctttcc gccttcctgc cgcaactcat tacgccgctt
      781 ttgacggcag gcggcattta tctgtgcttt gaaggcgtag aaaagctgct gcacaagttt
      841 ctgcaccgac atgacgcgca tgctcgtaat ggcggcaatc ggcatcggca tgactgtttt
      901 ggtgtacgga atcgttgccg tcatcgtcaa actcgacgac ttaggcatgc tgctgatgcg
      961 ccgcccacaa accttttcgc gcagtttggg gcaaatgctg actgccttta tgccttgctt
     1021 tatgcgcggt ttgagcgtgg tcggcacgct tgccatgttt cttatcggcg gcgtactggt
     1081 cgcccacaac ctcggccttc tgcacgattt cctgcacgcg caacactggg acgctggctg
     1141 ggcggagtac ttcgccaacc tcgttgtcgg gctgctttcc ggttcgattg cctgcgcccc
     1201 cgccttgccg ctgatgaatc gtttcggcag gcattgattt ctttttcaaa taccgatgcc
     1261 gtttgaaaga tgttcagacg gtatcttccg aacagacaga tgaatatggt ttccaaactg
     1321 gacaaatact ggcagcaccc cgccctctac tggcctttgc tcatcctttt tgccgccgcc
     1381 acccccttta ccttcgcacc ctactaccac ttttggctga tgcccttgat tttcggtgcc
     1441 ttcgtccgcc tcatcgaact gcgtccgcgt tttgctgtct cttccgccta cctgttcggc
     1501 ctgaccgcat acacgacaca gttctactgg atacacaccg ccctgcacga cgtttccggc
     1561 ctgcccgacc tctatgccgt accgctgacc ttcctactcc ccgcctacct tgccctttat
     1621 ccggcactgt gtttctggct gtggaaaaaa tttaccctgc ctcggggcat aaaaatcggt
     1681 ttggtactgc ccatcctgtg gacactgacc gagtttgccc gcgaacgttt cctgaccgga
     1741 ttcggctggg gcgcaatcgg ctactcccaa atcaccccgg acagcccgct cgccggcttt
     1801 gccccattgg gcggcatcca catggttaca ctggcaaccg cctttctcgg tgtctggctg
     1861 gttttggcga gtgataacac cgcacgttcg ggcaaacgcc tgcttccaat tatcctgatt
     1921 gccgccctgc ttgccgcagg ctacaccgcc cgacaaaccg acttcacccg ccccgacggc
     1981 agccgcagca ccgtcgccct gcttcaaggc aacatcgacc aaaccctcaa atggcgtgaa
     2041 gaccaagtca tcccgaccat acagaaatat tacgaacagg tcggcaaaac cactgccgac
     2101 atcgtcatcc tgcccgaaac cgccatcccc gttatgcgcc aaaacctgcc ggaaaacata
     2161 ctggcaaaat ttgccgaaca ggcgcaaaac aacggcagcg cgctcgccgt cggcatcagc
     2221 caatacactt cggacggcaa cggttacgaa aacgccgtca tcaacctgac cggttatcag
     2281 gaaaacaatc aggacggcat cccctactac gccaaaaacc acctcgtccc cttcggcgaa
     2341 tacaaaccgc tgcccttcct gaccacgccg ctttacaaaa tgatgaatat gcccctttcc
     2401 gacttccgca aaggcggcgg taagcaatcc gccctgctga tgaaaaacca aaaaatcgcc
     2461 ttcaacatct gttacgaaga cggattcggc gacgaactga ttgccgccgc caaagatgcc
     2521 acactgcttg ccaatgccag caatatggcg tggtacggaa aatccaacgc catgtaccag
     2581 cacctccaac aatcgcaggc gcgggctatg gaactcggac gctatatggt ccgtgccacc
     2641 aacaccggcg caaccgccat catctccccc aaaggcaaca tcatcgccca agcccaaccc
     2701 gatacggaaa ccgtattgga aggacacatc aaaggctatg tcggcgaaac gccctatatg
     2761 aaaaccggca gttcatggtg gttgatgggc atattgaccc tagccgcact gattcttttc
     2821 atcttccgaa acaaagaaca ctgatacctg cacagcccgt ccggacgggc tgtctttttt
     2881 caaaacggct atttacatac aattacgtat gtaatataat tctgaaatcg aattgagcca
     2941 tcaggtttac aaaatcaatc aaacctgcct tacaaaattt tcctgtcttt caaaccttag
     3001 cgcatatctc ctgccaaaca tacaaaatac aagccaagca gtgatatact gcatcccaac
     3061 acagcaaacc gccgatacgc agtttcagcc gaaaggaaat gccccaaatg aataacgctt
     3121 tcgcattacc cgccatccaa agcggcaacg gcagcctcga acaatacatc cacaccgtca
     3181 acagcatccc gatgctgtcc caggaagaag aaacccgcct cgccgaacgc cggataaagg
     3241 gcgacctcaa cgccgccaaa caactcatcc tgtcccacct gcgcgtcgtc gtttccatcg
     3301 cgcgcggcta tgacggctac gggctgaatc aggcagacct gattcaggaa ggcaatatcg
     3361 gactgatgaa ggcggtcaaa cgctacgagc ccggtagagg cgcgcgtctg ttttcatttg
     3421 ccgtacactg gattaaagcc gaaatccacg agtttatttt gagaaactgg cgtttggttc
     3481 gcgttgccac caccaaaccg caacgcaagc tgtttttcaa tttacgcagt atgcgtaaaa
     3541 acctcaacgc cctgtctccc aaagaggcac aagacatcgc cgacgatttg ggtgtcaaac
     3601 tttccgaagt tctggaaatg gaacagcgca tgacgggaca cgacatcgcc atcatggcag
     3661 acaacagcga cgacgaggac agtttcgccc ccatcgactg gcttgccgac cacgattccg
     3721 aaccgagccg gcaactatcg aaacaggcgc attacgccct gcaaacagaa ggtttacaaa
     3781 acgctttggc gcaactggat gacaggagcc gccgtattgt ggaaagccgc tggcttcaag
     3841 acgacggcgg gctgacgctg caccagcttg ctgcagaata cggcgtctct gccgagcgca
     3901 tccgccagat tgaagcaaaa gccatgcaga aactgcgtgg tttcctgacc gaagaagccg
     3961 aagcggtttg aaatatacgc aataaaatac ccgacaaatt gttttgtcgg gtattttatt
     4021 tggtaaacat caatgcggtt tgtgcgtcgt gctgtacacg attttatccg tccacgccgt
     4081 tgcaattgcc gaaatcaaaa agcagacatg caccagacac tgccgcatca gctgcttttc
     4141 cgacagattg gcggaattga tatagtggat taacaaaaat caggacaagg cg
//
LOCUS       DAWFAD010000152         4130 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWFAD010000152 DAWFAD010000000
VERSION     DAWFAD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.152"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            43..675
                     /locus_tag="WGO49_001772"
     CDS             43..675
                     /locus_tag="WGO49_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ0306847.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="WGO49_001773"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="WGO49_001773"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ0306848.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAGTLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKILQAQEFLGKINGAVGNYNAHMA
                     AYPDVDWETHCHNFVEIGLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLAEKLPVSRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKVFIGSLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2198..4066
                     /locus_tag="WGO49_001774"
     CDS             2198..4066
                     /locus_tag="WGO49_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0306849.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSRFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTASGKRQLLSLLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDTTLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFQARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLVKDIPNV
                     HWVDAQKYLPKNTVEIHGRYLYGDQDHLTYFGSYYMGREFHKHERLLKSSRGGALQ"
BASE COUNT          935 a         1113 c         1075 g         1007 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcgggc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 acggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttcagag tacggcctga
      841 tgaaggcgcg cgtcaaagtc gaattaaact ggctcaaagc cctggccgcc gagccgaaga
      901 ttgtcgaagt gccgcccttc agtgccggaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg ctgaaaaaac gttttgccga agtgccggaa gtcgccgccg
     1081 tgagtgagtt catccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa gctggccgaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggcc
     1261 agcccgccac gcccacaaca ctgggcaaag aaaccgccaa cgtcgtgtac cgcctgcaac
     1321 gccagttcaa aatccttcag gcgcaagaat tcctcggcaa aatcaacggt gcggtcggca
     1381 actacaatgc ccatatggcc gcttatcccg atgtggattg ggaaacccac tgccacaact
     1441 tcgttgaaat cggcctcggt ttgaccttca acccctatac catccaaatc gagccgcacg
     1501 actatatggc tgaattcttc caaaccctca gccgcatcaa cacgattctc atcgacttta
     1561 accgcgacgt ttggggttat atttcattgg gttacttcaa acaaaaagtc aaagcgggcg
     1621 aagtcggctc ttccacgatg ccgcacaaag tcaaccccat cgattttgaa aactccgaag
     1681 gcaacctcgg tatggcaaac gccgtattgg gttttttggc ggaaaaactg cccgtctcac
     1741 gctggcagcg cgatttgacc gacagcaccg tcttacgcaa tatgggcgtg ggcgtgggct
     1801 atgccgtgtt gggctttgcc gcccacctgc gcggtctgaa caagctcgaa cccaaccctg
     1861 ccgcgcttgc cgccgatttg gatgccactt gggagctgct cgccgagccg attcaaaccg
     1921 tgatgcgtcg ttacggcgtg gccaatcctt acgaaaagct caaagacctg acgcgcggca
     1981 aaggcggcat tacgcccgaa gtgctgaaag tcttcatcgg atcgttggaa attccggcag
     2041 aagccaaagc aaaattgctt gagctgactc ctgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacgggggc gatatttatg caagctgtcc gatacaggcc
     2221 tgaaattgac ggattgcggg ccgttgccgt gctatccgtc attattttcc acctgaataa
     2281 ccgctggctg cccggagggt ttttgggggt ggacattttc tttgtcatct cgggattcct
     2341 cattaccggc atcattcttt ctgaaataca gaacggttct ttttctttcc gggattttta
     2401 tacccgcagg attaagcgga tttatcctgc ttttatcgcg gccgtgtcgc tggcttcggt
     2461 gattgcctct caaatcttcc tttacgaaga tttcaaccaa atgcggaaaa ccgtggagct
     2521 ttctgcggtt ttcttgtcca atatttatct ggggtttcag caggggtatt tcgatttgag
     2581 tgccgacgag aaccccgtac tgcatatctg gtctttggcg gtagaggaac agtattacct
     2641 cctgtatcct cttttgctga tattttgctg caaaaaaacc aaatcgctac gggtgctgcg
     2701 taacatcagc atcatcctat ttctgatttt gactgccaca tcgtttttgc caagcaggtt
     2761 ttataccgac atcctcaacc aacccaatac ttattacctt tcgacactga ggtttcccga
     2821 gctgttggca ggttcgctgc tggcggttta cgggcaaacg caaaacggca gacggcaaac
     2881 agcaagcgga aaacggcagt tgctttcatt actctgcttc ggcgcattgc ttgcctgcct
     2941 gttcgtgatt gacaaacaca atccgtttat cccgggaatg accctgctcc ttccctgcct
     3001 gctgacggca ctgcttatcc ggagtatgca atacgggaca cttccgaccc gcatcctgtc
     3061 ggcaagcccc atcgtatttg tcggcaaaat ctcttattcc ctatacctgt accattggat
     3121 ttttattgcc ttcgcccatt acattacagg cgacaaacag ctcggactgc ctgccgtatc
     3181 ggcggttgcc gcattgacgg ccggattttc cctgttgagc tattatttga ttgaacagcc
     3241 gcttagaaaa cggaagatga ccttcaaaaa ggcatttttc tgcctctatc tcgccccgtc
     3301 cctgatactt gtcggttaca acctgtacgc aagggggata ttgaaacagg aacacctccg
     3361 cccgttgccc ggcgcgcccc ttgctgcgga aaatcatttt ccggaaaccg tcctgaccct
     3421 cggcgactcg cacgccggac acctgcgggg ttttctggat tatgtcggca gccgggaagg
     3481 gtggaaagcc aaaatcctgt ccctcgattc ggagtgtttg gtttgggtgg atacgacact
     3541 ggcagacaac ccgttatgtc gaaaataccg ggatgaagtt gaaaaagccg aagccgtttt
     3601 cattgcccaa ttctatgatt tgaggatggg cggccagccc gtgccgagat ttgaagcgca
     3661 atccttccta atacccgggt tccaagcccg attcagggaa accgtcaaaa ggatagccgc
     3721 cgtcaaaccc gtctatgttt ttgcaaacaa cacatcaatc agccgttcgc ccctgaggga
     3781 ggaaaaattg aaaagatttg ccgcaaacca atatctccgc cccattcagg ctatgggcga
     3841 catcggcaag agcaatcagg cggtctttga tttggttaaa gatatcccca atgtgcattg
     3901 ggtggacgca cagaaatacc tgcctaaaaa cacggtcgaa atacacggac gctatcttta
     3961 cggcgaccaa gaccacctga cctatttcgg ttcttattat atggggcggg aatttcacaa
     4021 acacgaacgc ctgcttaaat cttcccgcgg cggcgcattg cagtagcctg ccttcttgtc
     4081 ggatattgcc tttggcagcc tatgccgctg tttgcccttc ggggcggcgg
//
LOCUS       DAWFAD010000153         4098 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWFAD010000153 DAWFAD010000000
VERSION     DAWFAD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.153"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(202..1098)
                     /gene="argB"
                     /locus_tag="WGO49_001775"
     CDS             complement(202..1098)
                     /gene="argB"
                     /locus_tag="WGO49_001775"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ0306850.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGYIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKMGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDVGIGSMILGGGEDA"
     gene            1268..2398
                     /locus_tag="WGO49_001776"
     CDS             1268..2398
                     /locus_tag="WGO49_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ0306851.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFDNAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDHQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGVNLNREWENPTVEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANVTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAVLSAILNVAGDLR"
     gene            2471..3322
                     /locus_tag="WGO49_001777"
     CDS             2471..3322
                     /locus_tag="WGO49_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ0306852.1"
                     /translation="MITHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3405..4067)
                     /locus_tag="WGO49_001778"
     CDS             complement(3405..4067)
                     /locus_tag="WGO49_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ0306853.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
BASE COUNT          905 a         1195 c          996 g         1002 t
ORIGIN      
        1 aaaatcaaag ccggagaatt tatcccaaac aaccggattt caaaaaaacc agattcccgc
       61 cttcgcggga atgacggatt ttaggtttct gttttggttt ctgttttcgc gggaataaca
      121 acttagaaat tgcccgaaac cccaaaaaca gaaaccggac aaacgggttt tccgccaaag
      181 ccggcatttt ccgactttgc ttcaggcatc ttccccgccg cctaaaatca tcgaaccgat
      241 accgacatca gtaaagattt ccaacaaaag cgcgttgggc aacctgccgt cgatgatgtg
      301 cgtggctttc acaccgttga cggcggcttc gaccgcagaa gcgattttcg gcagcatacc
      361 gccatacagc gtgccgtcgg caatcagttc atcaatccgt ttcggcgtga gtttggtcag
      421 cagattgccc attttgtcca tcacaccggc gatattcgtc atcatcaaga gtttttcagc
      481 gttcaattct tccgccaatt tgcctgctac caaatcggcg ttgatgttga acgcttcgcc
      541 tttttcacct acgccgacgg gggcgacgac gggaatgtag ccgcgttcta tcagcccttt
      601 aaccaaaccg gtatcgatgc tttccaccgt accgacctgt ccgatgtcca cgccattctg
      661 ttcgggcgta tcgatcaaaa gtttcttcgc cttaatgaaa tgatcgtcgc gtccgctcac
      721 gccgaccgcg tgtccgccat atgtgttaat catcgacacg atttctttat tgacatgccc
      781 gcccaacacc atttcgacaa tatccatcgc ctctttgtcg gtaacgcgca ttccttggac
      841 aaactcaccc tttttgccga ctttttcaag catcgcattg atctgcggcc cgccgccgtg
      901 aacgatgacg ggatgaatgc cgaccagctt cagcagcacg acatcgcggg caaacccttc
      961 tttcaaggca ggttcggtca tcgcgttgcc gccgtatttg atgacggcga ccgaaccgga
     1021 aaaccggcgg atgtaaggca gcgcttcggc aaggatacgc gccttgtcgg cggcggaaat
     1081 aatgttttca gactccataa cggctcccta ttgttgacaa tttcggctta tgttaacgcc
     1141 aaacgccgtc tgaaacaatc ttcagacggc atccggcaga ctttcgggca ggttgaagcc
     1201 ttgcgatacg ggcggtaaaa tcgcggttta tccggttcga gaaaccggcg acagcaagga
     1261 ctttcccatg attaaaatca gcacccgctt cgatgccggc tcggtcgtcg tcaaagacct
     1321 gaccgatcct tccaacatcc gcctcgccct gcgtcccgac aacgcctccg attttgcaca
     1381 atggttctac ttccggctgc aaggcgcggc atatcaaaac tgcatcatgc attttgacaa
     1441 tgcggcagaa gccgcctacc cgaaaggctg ggaaggctat caggcgtgtg cctcatacga
     1501 ccgccgcaac tggttccgcg taccgacttc ctacgaaaac ggcgtactga ccgtcaacca
     1561 taccccgctg tccaatagcg tgtactacgc ctatttcgaa ccctattccg aagaacagca
     1621 cctcaacctc ctcggcgacg cgcaaggcag cggcctgtgc cgcatcgacg acttgggcag
     1681 caccgtgcaa gggcgcgaca tcaatctgct gaccataggc aaccaggtcg aaagcgattt
     1741 gaaaatctgg attaccgccc gccagcatcc aggcgaaacc atggcggaat ggtttgtcga
     1801 ggggctgctc ggcagactgc tcgaccatca ggatgcaacc gcgcgcaccc tgctcgatcg
     1861 cgccacgttc tatatcgtcc ccaacatgaa ccccgacggt tcggcactgg gcaaccttcg
     1921 caccaacgcc gccggtgtga acctcaaccg cgaatgggaa aatccgactg tggaaaaaag
     1981 ccccgaagtg ttcttcgtgc gtgaaagaat gctggaaacc ggcgtggatt tgtttttgga
     2041 tatccacggc gacgaagggt tgccctttgt ctttgttgca ggaactgaag gcgtgccgaa
     2101 ctacaatccg cgcatttccg cgttggaaac acagttcaaa accgcccttt tgaacgccag
     2161 ccccgatttc caagatgaat acggttacga aaaagatgcg cccggtcagg caaacgtgac
     2221 cttggcaaca aactgggtcg gcaaccgttt caactgcctt gcctatacgt tggaaatgcc
     2281 gttcaaagac aatgccaacc tgcccgacga cgacttcggg tggaacggtc aacgttcgtt
     2341 gcgcttgggc gaagcggtgc tttccgccat tttgaacgtc gccggcgatt tgcgttgaca
     2401 ctccccactg aaaaatgccg tctgaaagcc cttccccttt tcagacggca tttgaaaacg
     2461 aaagcccgtt atgattaccc atccccaatt cgaccccgtc cttatcagta tcggtccgct
     2521 tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta cctttctcgg
     2581 cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgcttg acgacttcct
     2641 cacttggggc attttgggcg taatttttgg tgggcgtttg ggttacgtcc tgttttacaa
     2701 gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag gcggaatgtc
     2761 gttccacggc ggctttttgg gtgtagttat tgccatatgg ttgttcggcc gcaaacacgg
     2821 catcggcttc ctcaaactga tggacacggt cgcaccgctc gttccgctgg gtctcgcttc
     2881 gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg acatcaacgc
     2941 attttgggca atgggcttcc cgcaggcgcg ttacgaagat gccgaagccg ccgcgcacaa
     3001 tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc cctcgcagct
     3061 ttatcagttt gcacttgaag gcatctgcct gttcgccgtc gtttggctgt tctctaaaaa
     3121 acagcggccg accggacaag tcgcctcgct cttcctcggc ggctacggca tattccgctt
     3181 cattgccgaa ttcgcacgcc aacccgacga ctatctcggg ctgctgacct tggggctgtc
     3241 gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct ttgtccggtt
     3301 cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca tggaaatccg
     3361 gtttcagacg gcatttttac gtttggttca tggaaacaaa cgcttcaatt cttaccgcaa
     3421 cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc tgttttttcg
     3481 ccttccctgc ggacggtttg cggcttgttg atgaccgcct gccagtagcg gtagatatcc
     3541 gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt gaattgcaga
     3601 cggatagtgc cgttgtcctc ttcgtcgtaa ataccgccca atgccatgat gggataaaac
     3661 aactcttcaa acgcttcatc atcgacggct tcaaaccaat cggtcggcac aatatccaaa
     3721 ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc gttttcatac
     3781 agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat cgccattgcc
     3841 aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc gtcgcccaac
     3901 acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag cgccgtcata
     3961 aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc atccaacaat
     4021 tcgctcaacc gccgtttgga tgcttcggta aatttttggg aatccatcat tttccttttc
     4081 aaatgggttt tgcgccct
//
LOCUS       DAWFAD010000154         4096 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWFAD010000154 DAWFAD010000000
VERSION     DAWFAD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.154"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1013..1555
                     /locus_tag="WGO49_001779"
     CDS             1013..1555
                     /locus_tag="WGO49_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0306854.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
     gene            1685..3346
                     /locus_tag="WGO49_001780"
     CDS             1685..3346
                     /locus_tag="WGO49_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ0306855.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEAGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENLGVEIYPGFAAAEVLYHKDGSVKGIATGNM
                     GVGKNGEPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGFQSLPKLSFKGGVLVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLENLEEVENFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGSPRLQINAANCVHCKTCDIKDPTQNITWICPESASGPNYGGM"
     gene            complement(3480..3974)
                     /locus_tag="WGO49_001781"
     CDS             complement(3480..3974)
                     /locus_tag="WGO49_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ0306856.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3971..>4096)
                     /locus_tag="WGO49_001782"
     CDS             complement(3971..>4096)
                     /locus_tag="WGO49_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0306857.1"
                     /translation="AGMAGLSGQISLTVPVLLTAQVLWVIIPLVLAAGIFRKRQI"
BASE COUNT         1030 a         1012 c         1093 g          961 t
ORIGIN      
        1 aatttcgatt taacttcggc acaccgtctc aacagcttaa aatcctgcaa aaatgatgtg
       61 gaatttaggg ctaatctagt acagcccctt agatttaatt cactatacct gcgtaatggt
      121 atcgggtact catcatcaac ctgccttgca ttcggttgtg ttgtccggca actgtccggt
      181 tgtgtcgatg ggttgccctg ccgctccctg tgctacgcaa cggttgtgtg ccttgggtgg
      241 gcgcgggtgg cggccgggcg gtttgttgct atgatacggg agttccgata tacaagggtg
      301 ggcttcagcc caccaatcaa cgaccgaaaa agaaaaaacg ttgggctgaa gcccactcta
      361 cgcaattacg caactgttat cgtggggatg acgggatttt aggtttttgt ttttctgttt
      421 ttgcgggaag gatgggtttt aggttgcggt catttatcgg aaaagcagaa atcgctccgc
      481 cgtcattccc gcgaaagcgg gaatctgaac acgtccgtag ggaaacctat atcccgtcat
      541 tcccacgaaa gtgggaatct agaacctctg aattttcaga caacctttga atattgccgc
      601 cgtcccgcgt tctagattcc cgcctgcgcg ggaatgacga ttcatcagtt tcccgaaacg
      661 cccgaaaaac cgaaaccgag caaaccggat tcccgcctgc gcgggaatga cgatttataa
      721 gtttcccgaa acgcccaaaa aaaccgaaat ccgacaggcg ggatttttgc tttcgtgggg
      781 atgacgggat tttaagttac ggtcatttat cggaaaagta gaaatcgctc cgccgtcatt
      841 ccagcgaagg cgggaatctg aacacgtccg cacggaaacc tatagtggat taacaaaaat
      901 caggacaagg cgacgaaacc gcagacagta cagatagtac ggcaaggcga ggtaacgccg
      961 tactggttta aatttaatcc actataatcc tgttgcttat ggaaaaatca aagtgcagag
     1021 ttggaaagag aggcgggatt tcaatattgt aaagcaggat ttggattttt cctgtggggc
     1081 ggcttcggtg gcgacgcttt tgaacaattt ttacgggcaa aagctgacgg aagaggacgt
     1141 gttaaaaaaa ctggataagg agcagatgcc cgcgtcgttt gaggatatgc ggcgcattat
     1201 gcctgatttg ggttttgaag cgaaaggcta tgccctgtct ttcgagcagc ttgcacagtt
     1261 gcaaatcccc gtcatcgtgt atctgaaata ccgcaaggat gatcatttct cggtattgcg
     1321 cggtatagac ggcaatacgg ttttgcttgc cgacccgtcg ctgggtcatg tttcaatgag
     1381 cagggcgcag tttttggatg cttggcaaac ccgtgaggga aatttggcag gtaagatttt
     1441 ggcggttgtg ccgaaaggga gggatgccgg cggggataag gcgtttttca cacgcagtcc
     1501 gaaacgtcag acggagtttg cggtcggaca ggtaaaatgg tggcgtgctt attgaaattt
     1561 cgacaaaggt cgtctgaaaa ccgaaaatat ggttttcaga cgacctttgt tgtatttggt
     1621 aactatatgt tctcgttgta taattacgga tttgcaattc aataataaat acacaggaac
     1681 cgccatgaca gaatccatca cccgcgacag tatgcaatac gatgtcgtga ttgtcggcgc
     1741 aggcccatcg ggtttgtccg ccgccatcaa actcaagcag cttgccgaac aaaatgggcg
     1801 cgaaatcagc gtttgcgtgg tggagaaagg ttcggaggcg ggtgcacatt cgctggcggg
     1861 cgcggttatc gatccgattg cgctgaatga gttgattccc gactggaaag aaaaaggcgc
     1921 gccgctgacg cgtacggtaa cgcaggacaa agtgttgttc ctgactgaga aaaaagcgtt
     1981 caatctgcct attaccccaa atttcgacaa tcacggcaac tacatcgtca gcttgggcga
     2041 agtcgtgcgc tggttggcgg agcaggcgga aaatttgggc gtggaaattt atccgggctt
     2101 tgccgctgcc gaagtgctgt atcacaaaga cggttcggtc aaaggcattg caaccggcaa
     2161 tatgggcgtg ggcaaaaacg gcgagccgac cgattcattc cagcccggca tggagctttg
     2221 ggcacggcaa accctgtttg ccgagggctg ccgaggttcg ctttccaaac aaatcatcga
     2281 acgcttcaaa ctcgaccaaa acagccagcc gcaaacttac ggcttgggca ttaaagaagt
     2341 ttgggaagtg ccgtccgaac agcatcagcc cggtttggtg gtgcacagcg caggctggcc
     2401 gctggacagc aaaacctacg gcggcgcgtt tgtttaccat ttcgacgaca acaaagttgc
     2461 cgtcggcttc gtggtcggtt tggactatca aaatccttat ctgtcgccgt ttgaagagtt
     2521 ccaacgtttc aaaacccatc ccgaaatccg caaaaccttt gaaggcggcc gccgcattgc
     2581 ttacggtgcg cgttcgctga ttgaaggcgg ttttcaaagt ttgccgaagc tctcgttcaa
     2641 aggcggcgtt ttggtcggcg atgcggcggg tttcctcaat atgccgcgca tcaaaggcat
     2701 tcataccgcg atgaaatccg ccatgctcgc cgccgaagcc gtgttcccct tgttggaaaa
     2761 ccttgaagaa gtggaaaact tcgacagcgg caaagaggcg gcggattatc agcaacgttt
     2821 tgaacaaagc tggctgtatc aagagcttta cgccgcgcgc aatgtccgtc cgtcattcaa
     2881 atggggcgtt tacctcggct caatctacac cggcatcgat cagatgattt tcagaggcaa
     2941 agccccgtgg accttgaaac atcacggcaa agacaacgag cagcttaaaa aagccgccgc
     3001 gtgcaagccg attgattatc cgaaacccga cggcgtattg accttcgacc gcttgagcag
     3061 cgtcttcctt gccaatctcg cgcacgaaga aaaccagccc gaccatttgg tgctgaacaa
     3121 tccgcaaacg atgatagacg tgaactacaa agaatacgcc tcacccgaaa cgcgctattg
     3181 tccggccggc gtgtatgaaa tcgtcgaaga aaacggcagc ccgcgcctgc aaatcaacgc
     3241 ggcaaactgc gtgcactgca aaacctgcga catcaaagac ccgacgcaaa acatcacttg
     3301 gatttgcccc gaaagcgcga gcgggccgaa ttacggcggg atgtaaacct tattccgccg
     3361 ttacgcccgg tcaggaataa ggaatggggc agttgttcaa acctgccccg tttgaaccta
     3421 tgacaaaaaa tgccgtctga accttggctt caacgttcag acggcatttt tgtttggatt
     3481 tacttgaaaa tataggtatc aggcatatcg tcgaaaccga caaccttacc gcctttatcc
     3541 tttgcaaatt tctcagcctg ctctttgttg ccgaacggca gcgcgtcttc cgcacccata
     3601 ccgccgataa agccgctgtc gatgacgtaa aaggcttttt tcgcatccat ccactccgtg
     3661 tcggcattgg gattcgtcca atcggtaaca ttgcccatat cggtaacgta aatcacgcgg
     3721 atgcctttgg gttcttcggg cagcttggta tagccgaaca tctgcttgat ggtggagaac
     3781 caaacgggct gatcgggttt gccgttcaag aaaatctggg ctttggggcc gttgtgttcg
     3841 gtcaggttca tactgcaata gtgtccgacc gaacggtcgc taatctgccg gggtaaaggc
     3901 ggcggcccct cttccgcctg ccggcaggca cttaaggcgg aaacggcaac aattgccaac
     3961 agggttttct tcatatttgt cgctttctaa aaattccggc tgccaaaaca agcggaatga
     4021 taacccataa aacctgagcg gtcagcaaaa cggggacggt caggctgatt tgtccgctca
     4081 aacccgccat acccgc
//
LOCUS       DAWFAD010000155         4060 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWFAD010000155 DAWFAD010000000
VERSION     DAWFAD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.155"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            38..461
                     /locus_tag="WGO49_001783"
                     /pseudo
     CDS             38..461
                     /locus_tag="WGO49_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            567..1130
                     /gene="pgsA"
                     /locus_tag="WGO49_001784"
     CDS             567..1130
                     /gene="pgsA"
                     /locus_tag="WGO49_001784"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ0306858.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1210..1285
                     /locus_tag="WGO49_001785"
     tRNA            1210..1285
                     /locus_tag="WGO49_001785"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1243..1245,aa:Gly,seq:gcc)
     gene            1311..1386
                     /locus_tag="WGO49_001786"
     tRNA            1311..1386
                     /locus_tag="WGO49_001786"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Gly,seq:gcc)
     gene            1410..1485
                     /locus_tag="WGO49_001787"
     tRNA            1410..1485
                     /locus_tag="WGO49_001787"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1443..1445,aa:Gly,seq:gcc)
     gene            1507..1582
                     /locus_tag="WGO49_001788"
     tRNA            1507..1582
                     /locus_tag="WGO49_001788"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1540..1542,aa:Gly,seq:gcc)
     gene            1608..1681
                     /locus_tag="WGO49_001789"
     tRNA            1608..1681
                     /locus_tag="WGO49_001789"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1640..1642,aa:Cys,seq:gca)
     gene            1784..1873
                     /locus_tag="WGO49_001790"
     tRNA            1784..1873
                     /locus_tag="WGO49_001790"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1818..1820,aa:Leu,seq:taa)
     gene            1977..3029
                     /gene="sohB"
                     /locus_tag="WGO49_001791"
     CDS             1977..3029
                     /gene="sohB"
                     /locus_tag="WGO49_001791"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ0306859.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3080..3943)
                     /locus_tag="WGO49_001792"
     CDS             complement(3080..3943)
                     /locus_tag="WGO49_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ0306860.1"
                     /translation="MENVETHSQNHRKRLIGTFFLVGLENLLMLVYPVFGGWAINAVI
                     AGQAWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAVT
                     ARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLPR
                     FAAISENLYFRLKNSLERDNHFIRKGDERQLDRHYGLLARLRVLISNREAFGYLCVGT
                     AMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQRI
                     EWSKRNIKAGT"
     gene            complement(3956..>4060)
                     /locus_tag="WGO49_001793"
     CDS             complement(3956..>4060)
                     /locus_tag="WGO49_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0306861.1"
                     /translation="KVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1024 a          965 c         1058 g         1013 t
ORIGIN      
        1 tcgctgggta tggtttgccg ccataagctg tctgctgttg tccgccgttt ttattgcacc
       61 ttacctgacg gcatttcacg aacaagaaaa aacattcgaa tatgcggaat tgaccgttac
      121 cgcccccaac cgcagcggac gggcaatcaa actggatgca gacgggcagc attaccggct
      181 ttcctgctat gggttcgaca gtttgtgcac aggcggcaat atcggcagaa ccatcagggc
      241 ggagcaggtt agagccgttt taagcgaaac cgtcggcaaa ggttttttaa acggcgttct
      301 gttggaatac cgcaacagtg gcagtgtcta taagcaataa agattttgcc cgcacggaag
      361 accgccttgt cgaagtgttg gcacaacctg ccgttttctg cctgaaactg ggtattttgc
      421 ttttattgcc cgccattttc ctgcgtttga aaagaatgtg aaaacagatt ggcggggcgg
      481 gggaatgccc atgcagcaat tttacgtttt gggttatgag gataaccgtt ataatcacaa
      541 ctttacagtc tgcacagaga aaaccgatgc cttggaatct ccctatcttc ctcacatggt
      601 tgagggtctt gctcattcct gtcatgacag ccctttttta cctgcctttt ccgtggttcg
      661 cggaggaaac gattaatctc accgccgccg tcatttttgc cgttgccgcc ttaaccgact
      721 ggtttgacgg atttttggca agactgtgga aacagacttc cgatttcggc gccttcctcg
      781 atcccgtcgc cgacaagctg atggttgccg tatcgctact tttactggtc aaactcgacc
      841 ggacctatgt tttgtttgcc atgatcatta tcggcaggga aattaccatt tccgcattgc
      901 gcgaatggat ggcgcaaatg ggcaaaagga acagcgttgc cgtcgccacc gtcggtaagt
      961 tcaaaaccgc cgcgcaaatg ctggcaattt tcctgctgct gctgaatatt cccgattttt
     1021 acggatttaa tcttgttgtt atcggcaatg tattgatgtt tatcgcatct ttgctgacag
     1081 tctggtcgat gctgtattat ctgaaaatgg cgtggaaaga aatcgcctga aaaaaacata
     1141 aaaatagctt gacggcaaaa acagaatcca taataattgc gtctcttcga tgttggagaa
     1201 tgaaatcggg cgggaatagc tcagttggta gagcgcaacc ttgccaaggt tgaggtcgcg
     1261 agttcgagac tcgtttcccg ctccaaaaat ttcattttcc cacaatcatt gcgggaatag
     1321 ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc gagttcgaga ctcgtttccc
     1381 gctccaagtt ttatttcaaa catatcaacg cgggaatagc tcagttggta gagcgcaacc
     1441 ttgccaaggt tgaggtcgag agttcgagac tcgttttccg ctccaaaaac cgatatgttt
     1501 gaacgcgcgg gaatagctca gttggtagag cgcaaccttg ccaaggttga ggtcgcgagt
     1561 tcgagactcg tttcccgctc caagtttttt agataagcca gttttagggc gagatagcaa
     1621 agtggttatg cagcggattg caaatccgtc tacgccggtt cgattccgac tctcgcctcc
     1681 attatcgtac tacggcgggg tggcagagtg tttatgctat gaggagtgca atcttcatat
     1741 aggccggtta aaatccgcgc ccccgcctcc acctttcaca aatgcccggg tggtgaaata
     1801 ggtagacaca acggacttaa aatccgtcgg gactaaacat cccgtgccgg ttcgattccg
     1861 gctccgggca ccaagctgaa aatgacaatg ccgctccaag cggttatttt tttatctgtc
     1921 ggacggtgat ggacaatatt gacatgttca tgcctgaaca agaggaaatc caatcaatgt
     1981 ggaaagaaat tttactgaat tacggtattt tcctgctcga actgcttacc gtgttcggcg
     2041 caattgcgct gattgtgttg gctatcgtac agagtaagaa acagtcggaa agcggcagtg
     2101 tcgtactgac ggatttttcg gaaaattata aaaaacagcg gcaatcgttt gaagcattct
     2161 ttttaagcgg ggaagaggca aaacatcagg aaaaagagga aaagaaaaag gaaaaggcgg
     2221 aagccaaagc agagaaaaag cgtttgaagg agggtgggga gaaatcttcc gaaacgcaaa
     2281 aatcccgcct ttttgtgttg gattttgacg gcgatttgta tgcacacgcc gtagaatcct
     2341 tgcgtcatga gattacggcg gtgcttttga ttgccaagcc tgaagatgag gttctgctta
     2401 gattggaaag tccgggcggc gtggttcacg gttacggttt ggcggcttcg cagcttaggc
     2461 gtttgcgcga acgcaatatt ccgctgaccg tcgccgtcga taaggtggcg gcgagcggtg
     2521 gttatatgat ggcgtgtgtg gcggataaaa ttgtttccgc tccgtttgcg attgtcggtt
     2581 cggtgggtgt tgtagcggaa gtaccgaata tccaccgcct gttgaaaaaa catgatattg
     2641 atgtggatgt gatgacggcg ggcgaattta agcgcacggt tacttttatg ggtgaaaata
     2701 cggaaaaggg caaacagaaa ttccgacagg aactggagga aacgcatcag ttgttcaagc
     2761 agtttgtcag cgagaaccgc cctcaattgg atattgagga agtggcaacg ggcgagcatt
     2821 ggttcggtcg gcaggcgttg gcgttgaact tgattgacga gatttcgacc agtgatgatt
     2881 tgttgttgaa agcgtttgaa aacaaacagg ttatcgaagt gaaatatcag gagaagcaaa
     2941 gcctgatcca gcgcattggt ttgcaggcgg aagcttctgt tgaaaagttg tttgccaaac
     3001 ttgtcaaccg gcgggcggat gtgatgtagt ttgccgaacg gatggaaatg ccgtctgaag
     3061 cgtgttcaga cggcattttt caagttccgg ctttgatgtt ccgtttcgac cactctatcc
     3121 gttgtccgat gtctttcaaa ttggaatatt gttcgaccaa tcgcggcacg tcgtccaaac
     3181 tcatggcaaa catccacaga taagtgccga ccgaatagac atgccccgcg ctgctgtagc
     3241 ctttgagcgt catcatcaca aaagcaaagc cgaacaaaat acccatcgcc gtgccgacgc
     3301 agagatagcc gaaggcttcg cggttggaaa tcagcacacg caggcgcgca agcagtccgt
     3361 aatggcggtc cagctgccgc tcgtcgcctt ttcggataaa gtggttgtcg cgttccaagc
     3421 tgttcttcag gcggaaatac aggttttcgc tgatggcggc aaaacgtggc aaaagccata
     3481 aaaacaacgc aagtatgccc accgccgaca cgccgaccca aaattccagc accagcagca
     3541 tgatgcacgc gccgaatatg gatacgacgg atgtcgcggc aatcggcagg tgttcttcaa
     3601 aaaagctgac aaactcacgc gacagggcaa cccgcgcagt taccgccgaa tgcgggactt
     3661 gccgctgccg ctgttccaac acaaccggca cggcgatttc ggtataaatc cgcgtaaacg
     3721 tgcgcgtatc ggcaatccgc cgcgccgcac cgaccagcca catcaaaagc acaaccaaag
     3781 cgtacagcaa cgcctgccac gcctgccccg caatcacggc attaatcgcc cagccgccaa
     3841 acaccggata caccagcatc aaaaggtttt ccagtccgac cagaaaaaat gtgccaatca
     3901 atcgcttgcg gtggttttgg ctatgtgttt caacattttc cacatatttg cccccctatt
     3961 ttttattttc cgagattgtt ttttccatca cttcagccag tgcatccaaa tcggcaaaca
     4021 gccgggttgt gcgcttgtcg ccgaatgtgg caaatacttt
//
LOCUS       DAWFAD010000156         4037 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWFAD010000156 DAWFAD010000000
VERSION     DAWFAD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.156"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(98..2944)
                     /gene="uvrA"
                     /locus_tag="WGO49_001794"
     CDS             complement(98..2944)
                     /gene="uvrA"
                     /locus_tag="WGO49_001794"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ0306862.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMESGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLATGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPEKVKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAQCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGIPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADYIVDLGP
                     EGGDGGGRIIASGSPEEVAKVKGSYTGKYLKVVL"
     gene            3099..3896
                     /locus_tag="WGO49_001795"
     CDS             3099..3896
                     /locus_tag="WGO49_001795"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ0306863.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
BASE COUNT          828 a          978 c         1210 g         1021 t
ORIGIN      
        1 aaattattaa gaaacattga taatgaatta ttcgatgtaa ttaaactttc atcaatagca
       61 ctaaaaaaat aatttgaact cattttaaat ctcctaatta caaaaccact ttcaaatact
      121 tcccagtata actcccctta accttcgcca cttcctcagg gctacctgaa gcaataatcc
      181 ttcccccacc gtcgccgcct tccggcccca aatccacaat ataatcagcc gtttttataa
      241 cgtcaagatt atgctcgata atcacaattg agttgccttt gcctttcaga cggcctatca
      301 cttccagcag cagggcgatg tcggcgaagt gtaggccggt ggtgggttcg tcgaggatgt
      361 agagcgttct gccggtgtcg cgtttggaga gttccaaggc gagtttgacg cgctgggctt
      421 cgccgccgga gagggtggtg gcggactgac cgaggcggat gtagcctagg cctacgtcca
      481 tcagggtttg cagtttgcgc gatacggtgg ggacggcatc gaaaaattcg cgggcttctt
      541 cgacggtcat gtcgaggact tggctgatgt ttttgccttt gtattggatt tcgagggttt
      601 cgcggttgta gcgtttgccg tggcagactt cgcaggggac gtacacgtcg ggcaggaagt
      661 gcatttcgac tttaatcacg ccgtcgcctt ggcaggcttc gcagcggccg cctttgacgt
      721 tgaaggaaaa gcggccgacg ttgtagccgc gttcgcgcga gagggggatg ccggcgaaga
      781 gttcgcggat gggggtgaac aggccggtgt aggtggcggg gttggagcgc ggggtgcggc
      841 cgatggggga ttggtcgacg ttgatgactt tgtcgaggtg ttcgaggccg cggatgtcgt
      901 cgtatggggc gggttcttct tgggcgcggt tgagttcgcg ggcggtaatt ttggcgaggg
      961 tgtcgttaat cagggtggat ttgccgctgc cggatacgcc ggtgatgcag gtaatcaaac
     1021 cgagcggcag ctcaagggta acgtttttga gattgttgcc gcgtgcgcct ttgaggacga
     1081 gcatccggtc gggattgacg ggcgtgcgtt cagacggcac ggcaatggat tttttgccgc
     1141 tgaggtattg tccggtaacg gagttttcgc attgggcgac gttttcgggc gtgtcggcaa
     1201 tcagtacgtt gccgccgtgt tcgcctgcgc cggggcccat atcgaccacg aaatcggctt
     1261 cgcggatggc gtcttcgtcg tgttcgacca caatcacgct gttgcccaaa tcgcgcagtc
     1321 gtttgagggt ggccagcagg cggtcattgt cacgctggtg caggccgatg gagggttcgt
     1381 ccagtacata catcacgccg gtcaggccgc tgccgatttg gctggcgagg cggatgcgct
     1441 gggcttcgcc gccggagagg gtttcggcgg agcggcttaa attcaggtaa tccagcccga
     1501 cgttaatcag gaagccgagg cgttcggtga tttctttgag gattttttcg gcgatttgtt
     1561 ttttgttgcc gtctaaatcc agtgtttcaa agaattggtg ggttttggtg agcggccagg
     1621 cggaaacttc gtgcagcggt tcgccgctga cgtaaacgta gcgggcttct ttgcgcaaac
     1681 gtgcaccacc gcagctcggg caggcgcggt ggttctggta ttcgcgcaat ttttcgcgca
     1741 cggtttcgct gtcggtttcg cggtagcggc gttcgagatt gggaatgatg ccttcgaagg
     1801 cgtggctgcg gttgaaggtg gtgccgcgtt cggataggta ggtgaaatca atgacttcct
     1861 tgcccgagcc gtgcagcacg acttttttga ctttttcagg cagcgtttcc caagcagcct
     1921 gcacatcgaa accgtaatgc cgcgccagcg actgaatcat ttggaaatag aactggttgc
     1981 gcttatccca gccgtcaatc gcgcccgttg ccagcgacaa ttcgggatgc gcgaccactt
     2041 tttcgggatc gaagaagttg gtgttgccca agccgtcgca agttgggcag gagcccatcg
     2101 ggttgttgaa cgaaaacagg cgcggctcta attcgggcag gctgtatgaa catacgggac
     2161 aggcgaaacg cgcggaaaac caatgttctt cgccgctttc catttccatc gccagcgcgc
     2221 gctcgttgcc gtggcgcagt gcggtttcaa aactttccgc cagccgctgc ttgatgtccg
     2281 ccttcacttt cacgcggtcg atgaccacgt cgatattgtg cttgatgttt ttctccagct
     2341 tcggcacttc gtcaagctga taaacttcgc cgtccacgcg cacacgggca aagccttgcg
     2401 cctgcaagtc ggcaaagaag tcgacaaact cgcccttacg ctctcgtacc gccggcgcca
     2461 gaatcatcac gcgcgtgtct tccggcagtt ttaagacggc atcaaccatt tgagatacgg
     2521 tttggctgga tagcggcagc ttgtgttcgg ggcagtaggg tgtgccgacg cgtgcgtaca
     2581 aaaggcgcag atagtcgtgg atttccgtaa ccgtgccgac ggtggaacgc ggattgtggc
     2641 tggtggattt ctgctcgatg gaaatcgcgg gcgacaggcc ttcgatcaaa tcgacatcgg
     2701 gtttgtccat catctgcaaa aactgccgcg cataggcgga caggctctcg acataacgcc
     2761 gctgtccttc ggcatacagc gtgtcgaacg ccagcgatga tttgccgctg cctgacaatc
     2821 ctgttaccac caccagcttg tggcgcggaa tgtctaaatc gacgtttttc aaattatgcg
     2881 tgcgcgcgcc gcgtatgcgg atggtgtcgt tgtcgtgcga atgttgggga tgatggttgc
     2941 acataatgga tgccgcctga aaaataaagg aaaaccgata ttgtagcact ttatcaaata
     3001 acgtcttaag caggattcag acggcatttg tcggcggagt acggcagatt ccgctataat
     3061 gtcggcaatt ttaacccgct tgaacaaaag gatgacaaat gaaccgtctt tacccccacc
     3121 cgattatcgc ccgtgagggc tggccgatta ttggcggcgg tttggctttg agcctgctgg
     3181 tgtcgatgtg ttgcggctgg tggtctttgc cgttttgggt gtttaccgta tttgccctgc
     3241 agtttttccg cgaccctgcg cgtgagattc cgcaaaatcc tgaagcagtg ttgagtccgg
     3301 ttgacggccg tatcgtggtg gtcgagcgcg cacgcgatcc gtatcgtgat gtcgatgctt
     3361 tgaaaatcag tatttttatg aacgtgttca acgtgcattc gcaaaaatcg cctgccgatt
     3421 gtacggtaac gaaagtggtc tataacaaag gcaaattcgt gaatgcggat ttggacaaag
     3481 ccagcacgga aaacgaacgt aatgcggtgt tggcgactac ggcttccggt cgtgaaatta
     3541 cttttgttca agtggccggt ttggtggcgc gccgtatttt gtgctacacc caagcaggtg
     3601 cgaaactgtc ccgcggcgaa cgttatggct ttatccgctt cggttcgcgc gtggatatgt
     3661 atctgcctgt cgatgcgcag gcgcaagtgg cgattggcga taaagtaact ggcgttagca
     3721 ctgtattggc gcgtttgccg ctgactgcgc cgcaaatcga atccgagcct gagtctgagc
     3781 ctgctttaca aactgctccg gttgaaacag cggcaaaccc atctgccgaa caacggcaaa
     3841 tcgaggcagc ggcggctaag attcaggcgg ctgtgcaaga tgtgttgaaa gattaatttt
     3901 gcgaactgaa atagaaaata tcagtaccat cattcacacg aatgaggaag tttggttttt
     3961 tgaatttttg ctaatgttca caccgttatg ttcacgaaaa tgggaatctg gaaatttaac
     4021 gttacggtga tttatca
//
LOCUS       DAWFAD010000157         4022 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWFAD010000157 DAWFAD010000000
VERSION     DAWFAD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.157"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(214..285)
                     /locus_tag="WGO49_001796"
     tRNA            complement(214..285)
                     /locus_tag="WGO49_001796"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Arg,seq:cct)
     gene            complement(305..751)
                     /locus_tag="WGO49_001797"
     CDS             complement(305..751)
                     /locus_tag="WGO49_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ0306864.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(756..1730)
                     /gene="ispB"
                     /locus_tag="WGO49_001798"
     CDS             complement(756..1730)
                     /gene="ispB"
                     /locus_tag="WGO49_001798"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ0306865.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDGKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVDSDSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVAALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(1746..1934)
                     /locus_tag="WGO49_001799"
     CDS             complement(1746..1934)
                     /locus_tag="WGO49_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306866.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLMEA"
     gene            1954..2262
                     /gene="rplU"
                     /locus_tag="WGO49_001800"
     CDS             1954..2262
                     /gene="rplU"
                     /locus_tag="WGO49_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ0306867.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2287..2559
                     /gene="rpmA"
                     /locus_tag="WGO49_001801"
     CDS             2287..2559
                     /gene="rpmA"
                     /locus_tag="WGO49_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ0306868.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            2794..3978
                     /gene="ubiM"
                     /locus_tag="WGO49_001802"
     CDS             2794..3978
                     /gene="ubiM"
                     /locus_tag="WGO49_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ0306869.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNVSILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSNKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT         1150 a         1110 c          874 g          888 t
ORIGIN      
        1 aatttaatcc actatatcat aaaacaacag atttccaagc cggaacatct tttacgaagc
       61 ctgaaaatca tttcatgatt ctaccgtcct aaaggtcggt ttttcaagca ggaagaaaaa
      121 gttttcagat ggcaaaaaag ccctccaaca ctgaaaggcc ttatatcgga aacttcccgc
      181 aacacgggaa acagacaaat gcaaatcgtc aaacctcgcc aacaggaatc gaacctgtat
      241 tttacgctta ggaggcatac gttctatccg ttgaactatg gcgagccgaa atgaaaagga
      301 gattttaacc ctttccgacg acaaaagaca agatgccggc cgcaatcagc ggcccgacag
      361 ggataccgcc cataaatgcc accccgataa ccgtcccgat taacagccct gtaattaaaa
      421 caggctgctg tcccatcaaa ggcacgccgc gtcccgccag ccaagccacg aaaataccga
      481 taaaaacggc ggatatcatt ttaaaattca aaaattcggc aacgggagga acctgcgcct
      541 ttcctgaaac caacggactc aaaaccccta tggtcaaaag aatgataccg agattcaacc
      601 catgcttctc gaccaacggg acaaactgta ccaatgccgt ctgctgcatc agcagcaata
      661 tggttgccga gatggtaatc gaattgttgt tgctgaccac ccccaacaga atcagcgtaa
      721 ccagaaacag gggaacaaaa ctgaaattca tcgcctcaag acaccctgac caaagattcc
      781 ttcgccagct gaatcatggc atccttcact tcgctgtcgg gcagggcatc caaggcggca
      841 acggcacaat cgactgcttt gcgcgcctcg cctattgaat atgccaaagc atccgaacgg
      901 acgacataat cgtggatttt ctcaaaatag ctgcgatctg cattttccaa agcagtacgc
      961 acatcgttcg caacctgttc ggaaccctga cgcatcagat aaatcaaagg caaagtcggt
     1021 tttccttccg ccaaatcgtc gccgacgttt ttgccggttt cttcggtttc gccagaatag
     1081 tccagcacat cgtcaataat ttggaatgcc gtaccgacat acataccgta gtctttcaac
     1141 gcccgttcgt gttcggggga agccttgccc aaaattgcgc cgacttgagc ggcagcttca
     1201 aacaattttg ccgttttata ttggatgact tggatatatt gttcttcggt aatgtccgta
     1261 ttgccgatgt tcatcagctg catgacttcg ccctcggcaa taatgttggt tgcatccgcc
     1321 atcacttcca aaacgcgcat actgtccgag tcaaccatca gttgaaaggc gcgcgtgtat
     1381 aaaaagtcgc caaccaacac tgccgccgca ttgccgaaca gattgtttgc cgttgcccgt
     1441 ccacggcgca aatcgctttc atcgacgaca tcgtcgtgca gcagcgtcga agtgtggata
     1501 aattcgacca tcgccgccag cgaatacagt ttcccgtcat cataaccgac cgccttaccc
     1561 gccaaaatcg tcataatcgg acgcaggcgt ttgccgcccg cgctgatgat atatgtaccg
     1621 atttgcgaaa tcagtgcgac atcggattgc accgcacggt tgatgacttc attgactttg
     1681 gcaaggtctt caggcagatg tcgctggaaa tagggcagat tttcgagcat ggcagactct
     1741 caggtttacg cttccatcaa acggcagata ccgcttgaaa gctgattcaa atagcgggca
     1801 attatatcag caaagccatc agggtacatc ttttcagacg gcatatggtt atccgaaccg
     1861 cgcaaacttt gacaaaacag gcaaataaca atacaatgcc gagttccgca caatggtgtg
     1921 cggatattaa ccataaccct tatggagttg agtatgtacg cggtcgtaaa aaccggcggc
     1981 aaacagtata aagtttccgt cggcgaaaaa ttgaaagtag aacagatacc agccgaactc
     2041 gacagccaaa tcgaactgac cgaagttttg atgattgctg acggcgaatc tgtaaaagtt
     2101 ggcgcaccct ttatcgaagg tgcaaaagta acggctaaag tagtggcaca cggtcgtggc
     2161 gaaaaagtcc gcatcttcaa aatgcgccgc cgcaaacact accaaaaacg ccaaggccac
     2221 cgccaaaatt tcacccaaat cgaaatcgtg gcaatcgcct aaccttaaaa cttaggagca
     2281 ttacaaatgg caagtaaaaa agcaggcggc agcacccgca acggtcgcga ttcagaagcc
     2341 aaacgcttgg gcgttaaagc ctacggcaac gagctgattc ccgcaggttc catcatcgta
     2401 cgccaacgcg gtaccaaatt ccacgcaggc gacaacgtag gcatgggcaa agatcacact
     2461 ttgttcgcca aagttgacgg ttatgttgaa ttcaaaacca aaggtgcgct gaaccgtaaa
     2521 actgtcagca tccgtcctta caccggttct gaagaataat ccaaccgata cctgaagccg
     2581 catcttttca cgatgcggct ttttacatac cccacacccg atttgataca cattaagctg
     2641 aaagtaaaaa tccgcataca tcctcccctg catatttctt caacagcggg ttttgatata
     2701 atcgcctatc tgttacagat agttcaaacg gcattcaaac ccttacaaat gccgtctgaa
     2761 atcatcggca tccgtctatg caaaggatat tttatgagtt tacacagcga catcctcgtc
     2821 gtcggcgcag gcccggcggg tttaagtttt gcagccgaac ttgccggcag cggtttgaaa
     2881 gttaccctga tcgaacgcag cccactgact gttctgcaaa atcctccata tgacggacgc
     2941 gaaatcgccc tgacccattt ttcccgcgaa atcatgcagc gtttgggtat gtgggacaaa
     3001 attcccgaaa acgaaatcta ccctttgcgc gatgccaaag tgctgaacgg acgttccgac
     3061 taccagctcc actttcccca accgactgaa gcgcgcggcg aacctgccga ctgtttgggc
     3121 tatctgattt ccaaccacaa tatccgccgc gccgcctatg aagtcgtatc acaactcgac
     3181 aatgtcagca ttctgaccga tacggttgtc aaagaagtca aaacatctga caacgaagca
     3241 caagtcattt tggaaaacgg caaaatcctg actgcacgcc tgcttttggc ggcagacagc
     3301 cgcttctcgc aaacccgtcg acaactcggt atctcctccg atatgcacga ttacagccga
     3361 accatgttcg tctgccgtat gaaacatacc ctttccaacc aacataccgc ttacgaatgc
     3421 ttccattacg gacgcaccat cgcactgctg ccgctggaag agcacctgac caataccgtc
     3481 attaccgtcg ataccgataa aatcaacagc gttcaaaacc tttcgcccga agaattggcg
     3541 gcaagcgtaa aagaacaact caagggtagg cttggcgaca tggaactggt cagcagcatc
     3601 caccattacc cccttgtcgg tatgattgcc aaacgtttct acggcaaacg cagcgcgctg
     3661 atcggcgatg cggcggtcgg tatgcaccct gtaacggcac acggcttcaa cttgggtctt
     3721 tccagcgcag atattctggc aaaactgata ctcgaagccg aacaacgcgg tcaggacatc
     3781 ggcgcatctt ccttgttgga aaaatacagc aacaaacata tgctgcacgc ccatccgctt
     3841 tatcacggaa ccaatatgat gctgaaactg tttaccaacg aaaccgcgcc ggcaaaactg
     3901 ctgcgcggtt tggtattgcg tgccggcaac aacttcccgc cgctgaaaaa gctgattacc
     3961 aaacaactga ccggttgaaa actgccgaaa tgccgtctga aacgtttcag acggcatttt
     4021 tg
//
LOCUS       DAWFAD010000158         3967 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWFAD010000158 DAWFAD010000000
VERSION     DAWFAD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.158"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            37..174
                     /locus_tag="WGO49_001803"
     CDS             37..174
                     /locus_tag="WGO49_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-like fold family protein"
                     /protein_id="HEZ0306870.1"
                     /translation="MHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHLEKVF
                     G"
     gene            complement(545..1585)
                     /gene="queA"
                     /locus_tag="WGO49_001804"
     CDS             complement(545..1585)
                     /gene="queA"
                     /locus_tag="WGO49_001804"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ0306871.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVTVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVVDRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGWNEGVVR"
     gene            1702..1959
                     /locus_tag="WGO49_001805"
     CDS             1702..1959
                     /locus_tag="WGO49_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ0306872.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPKHRQPLKEQKWICAN"
     gene            1941..2396
                     /locus_tag="WGO49_001806"
     CDS             1941..2396
                     /locus_tag="WGO49_001806"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ0306873.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEVGQ
                     QVKVGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            2508..3869
                     /gene="accC"
                     /locus_tag="WGO49_001807"
     CDS             2508..3869
                     /gene="accC"
                     /locus_tag="WGO49_001807"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ0306874.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIEAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAVYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIASGLPLQYKQKDIKIEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFNDPGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
BASE COUNT          976 a         1183 c          962 g          846 t
ORIGIN      
        1 gatttctttg aaggcaaagg cgttgatgtt ttgtatatgc acttccacaa agccaacgag
       61 tttttgggta tgacccgcct gccgacattc ttatgtaacg atgtggttaa aaatccgcaa
      121 gtggaaaaat acttggcaga ttatcaggca cacttggaaa aagtgttcgg ctaaaaattt
      181 atcttataaa caaacaaagg cagcctgaaa gattgaatgg tctgcacccc taaggttgga
      241 ctaaccaacc gactaaggtg cagattattt tttgttgctt tttcagcttt tcgttgggtt
      301 agatattctt gcccactgtt ttcaggcagc cttgaataca aaaaatggcg tatgtaatat
      361 gtttatacga ccaaaacgga atgaatttta acgtattgcg cgtcatcaac aatgactgag
      421 tttctcgcct ctcgcgcctg aatctatgtc atatacagtt cctttcattc aaatcaacaa
      481 aagaatgccg tccgaacgtc cgttcagacg gcacttgtct tcccgcaata gacttggggc
      541 tgttctaacg taccacccct tcgttccacc ccaaaaccat cgcatcgccg tagctgaaga
      601 aacggtattc gcgttcgacc gcataacgat acgcggcgcg gatatgaccc atacccgaaa
      661 acgcgctgac aagcatcagc agcgtcgatt tcggcaaatg aaaattggta accagcctgt
      721 cgacaacatt aaaacggtag cccggcgtga tgaaaatatc ggtgtcgccc tgccccgctt
      781 tcagacgacc cgtcgcacgc gcggcagatt cgagggcgcg catggaagtc gtgccgactg
      841 cccagacttt gttcccccgg gcttttgccg cctcaacggc ggcggcggtt tcagacggca
      901 cttcaaacca ttcgctgtgc attttatgct cttcgatttt gtccacacgc acgggttgga
      961 atgtccccgc accgacgtgt agggttactt ctgcggttac tgcgcctttg tctttcaggc
     1021 ggcgcaaaag ttcttccgta aaatgcagac ccgccgtcgg cgcggcgacc gcgccctgat
     1081 atttggcata aacggtttga taacggctgt cgtcatccgc atcggcggcg cgttcgatat
     1141 aaggcggcag gggcaggtgt ccgttctgtt ccaaaagttc gtaaacggtt tgaccgcctt
     1201 caaaacgcag gcagaacagt tcgccctcac gcccgaccgt cacggcgcgg atgccgcctt
     1261 caaacaccag ccccataccg ggcttgggcg atttggacga acggatgtgc gccagtgcgg
     1321 tatggttgtc caacacgcgc tcaatcaggg cttcgatcct gccgccgctg tctttctgcc
     1381 caaacagccg cgccttcatg actttggtgt tgttgaacac caaaacgtcg cccgcctcaa
     1441 cataatccgg caaatcgccg aacacccggt cttgcagcgg catatcgggc aacgcaacca
     1501 aaaggcggct gctgccgcgc acttcgggcg gatgctgggc aatcagcttt tcgggcaggg
     1561 taaaatcaaa atctgaaata tccattttta cactctcgtt cgggcaagcc gccattatac
     1621 gcactttagc cctttttcag acggcatctt tgtccgaaaa accaacagat tagaataaac
     1681 actcttaacc cggggcatct tgtgcgcaaa atcaaacttc ctgcacattt cccccaaaaa
     1741 ccgccgtttt ttgatatttt actggacatt taccgacaac ttcgggaaaa taaacacatt
     1801 ctcacggtcg ttttccacca caggaaaacc gtatccgaac accattccgc ccggtttgcg
     1861 ccgttgccgc aagccggctg ttttctgaaa aaccaacgca acaacccgcc gaaacaccgg
     1921 cagcctttaa aggaacagaa atggatttgc gcaaattaaa aaaactgatt gatttggttg
     1981 aagaatcggg tatcgccgaa atcgaagtaa ccgaaggcga ggaaaaagtc cgcatcaccc
     2041 gaaccatcgc cgccgcaccc gtttacgccg cgcccgtacc tgccgccgcg ccggccgtaa
     2101 cgcctgccgc cgcacccgtt gcggcatccg cgcctgccgc cgcacctgcc gcccgcgatt
     2161 tgtccgacgc gcaaaaatca cctatggtcg gcacgttcta ccgcgcaccc ggcccgaatg
     2221 ccgcgccttt tgtcgaagtc ggccaacagg ttaaagtcgg cgacacgctg tgcatcatcg
     2281 aagcgatgaa gctgatgaac gaaatcgaag ccgaaaaatc cggcacggtc aaagaaattt
     2341 tggtcgaaaa cggtacgccc gtcgaattcg gcgaaccgct cttcatcatc ggataatcct
     2401 gttttcagac ggcataaact tccgatgccg tctgaaatgc tttccccctt cagcgttccc
     2461 gcaccctttt ttacggacgg gttgccggaa ccgcaggaaa ggtcatcatg ctgaaaaaag
     2521 ttttaatcgc caaccgaggc gaaatcgcat tacgcgtact ccgtgcctgc cgcgaaatgg
     2581 gcattgccac cgtcgccgtg cattccgagg ccgacaaaga cagcctgcac gtcaaactcg
     2641 ccgacgaatc cgtgtgcatc ggccctgccg cttccgcgca aagctacctc aacgtccccg
     2701 ccattatcgc cgccgccgaa gtaagctgcg cggacgctgt ccacccgggc tacggtttcc
     2761 ttgccgaaaa cgccgatttc gccgaacagg tcgagcagtc cggctttacc tttatcggcc
     2821 cgaaacccga caccatccgc ctgatgggcg acaaagtatc cgccaaacac gccatgatcg
     2881 aagcgggcgt gccttgtgtt cccggctctg acggcgcatt gcccgacgac ggcgaagaaa
     2941 tcctcaaaat cgccgataaa gtcggttatc ctgtcattat caaagcctct ggcggcggcg
     3001 gcggtcgcgg tatgcgcgtg gtcgagaaaa aagaagacct cctccaatct gtcgaaatga
     3061 ccaaggcaga agcaggcgcg gcattcggca acccgatggt ttacatggaa cgctatttgc
     3121 aacgtccgcg ccacgtcgaa atccaagtga ttgccgacga acacggcaac gccgtctacc
     3181 ttgccgaacg cgactgttcg ctacaacgcc gccaccaaaa agtcatcgag gaagcaccgg
     3241 ctccgttcat cactgaaaaa gaacgcgcca aaatcggcaa cgcctgtgcc gacgcctgca
     3301 aacgcatcgg ctatcgtggc gcaggtacgt ttgaattcct gtacgaagac ggcgaatttt
     3361 tctttatcga gatgaatacg cgcgttcagg ttgagcatcc ggttaccgag ctcatcaccg
     3421 gcgtggacat tgtgcaggag caactccgca tcgcatccgg cctgccgctg caatacaagc
     3481 agaaagacat caaaatcgaa ggccacgcgt ttgagtgccg tatcaacgcc gaagacccat
     3541 acaacttcat ccccagcccg ggcccgattg aaagctgcca cctgcccggc ggcttcggta
     3601 tccgcgtcga cagccacatt taccaaggct accgcatccc gccttactac gacagcctga
     3661 tcggcaaaat ctgcgtacac ggcaaaacgc gtgaacaggc aatggcgaaa atgcgcgtcg
     3721 cactcgccga gctggcggta accggcatca aaaccaatac gccgcttcac cgcgacttgt
     3781 tcaacgaccc aggtttccaa aaaggcggcg tcagcatcca ctatttggaa cactggctgg
     3841 aagatcgcaa agccaaacag gacaaataag ccgccgccga tatgccgtct gaaacagttt
     3901 gtccgttttc agacggcatt tcccttgccc cgcgccgtct gaaaccgatt tcgatatagt
     3961 ggattaa
//
LOCUS       DAWFAD010000159         3925 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWFAD010000159 DAWFAD010000000
VERSION     DAWFAD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.159"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..331)
                     /locus_tag="WGO49_001808"
     CDS             complement(<1..331)
                     /locus_tag="WGO49_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0306875.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVKEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQ"
     gene            complement(312..593)
                     /locus_tag="WGO49_001809"
     CDS             complement(312..593)
                     /locus_tag="WGO49_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ0306876.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNKDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRQAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(602..1396)
                     /locus_tag="WGO49_001810"
     CDS             complement(602..1396)
                     /locus_tag="WGO49_001810"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ0306877.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1780..3114
                     /gene="gdhA"
                     /locus_tag="WGO49_001811"
     CDS             1780..3114
                     /gene="gdhA"
                     /locus_tag="WGO49_001811"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ0306878.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3256..3630
                     /locus_tag="WGO49_001812"
     CDS             3256..3630
                     /locus_tag="WGO49_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306879.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3674..>3925
                     /locus_tag="WGO49_001813"
     CDS             3674..>3925
                     /locus_tag="WGO49_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ0306880.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQ"
BASE COUNT         1068 a         1014 c          924 g          919 t
ORIGIN      
        1 cttgcgcgat gccgttcaag gacaaaccca tacccttttc ggcaaaacgg atttcacggt
       61 cgtcaaacga gactttgccg aagccgaccc gtttcagggc ttccttcacg ctgttttgag
      121 gaggcggcgt ttccgcatcg ggacgggcgg caaaataatc ggcatacagt ttccacaacg
      181 cctgcactgt cggatcgaac gcgccgtccg tcagcgcgtg aatatcgcgg cacaggctca
      241 acagttccaa aaaatccgcc gacggcgaag tcagataacc gtccctgttc aggcggctga
      301 tcaggctgtc ttcacggtaa aggctgaaca attttttcca aacgcgccac ttccgccaaa
      361 accttgttga ccaaatccgc cgcctgtctg tcgtccacac cgaacaggcg cagctccgca
      421 ccggaaccca gcgcgacacc tttccagaag aacacatttt cattgcgttt ttcatcttta
      481 ttgcgttttt catcatcggc ggcaaaagga ttcggcagga aagaaaccgc cgcgcccgcc
      541 gccgcaacgg cggcaactgt caaaaaacgc ctgcgcccga aatgcctgcc cataccgcct
      601 cctaaaccga cactgccgcc ttgatgtgcg gatgggggtc gtaaccttcc aactcgaaat
      661 cttcaaactt gaaggaaaac aaatctttga cttcaggatt gattttcatc actggcaagg
      721 cgcgcggttc gcgttccaac tgcaatgcgg cctgctcgaa atggttgcgg tacaaatgcg
      781 cgtcgccaaa cgtatggaca aactcgcccg cctccaatcc gcacacttgc gccatcatca
      841 tggtcaacaa tgcgtagctg gcaatattaa acggcacacc aaggaaaata tctgcactac
      901 gctggtaaag ctggcaggac agtttgccgt cggcaacgta aaactgaaac agcgcgtggc
      961 agggcggcaa ggccatttcg tcgaccaaag ccggattcca cgccgataca atcaggcggc
     1021 gcgagtcggg gtttttctta atttgttcca ccacattggc gatttggtcg atatgcctgc
     1081 cgtcgggcgc gggccagtta cgccactggt agccgtaaac cgggcccaag tcgccgtttt
     1141 cgtccgccca ctcgtcccaa atggaaacat tgttgtcctt taggtatttg atattggtat
     1201 cgcctttgag aaaccaaagc agctcgtgga taatcgaacg cagatgcagc tttttggtcg
     1261 tcagcagcgg aaaacctttg cccaagtcaa aacgcatctg ataaccgaat acggagcgcg
     1321 tgcccgtacc ggtgcggtct gatttgtccg taccgttgtc gaggacgtgg cgcatcaagt
     1381 ccaaataggc tttcatagca gtctttcatc aaattaaacg gcgcatattg taacatttcc
     1441 ggataatgcc caaaacacgg atacagacag gcaggattgt tggcaatttc agtccttttc
     1501 cacagtaaaa cccggtggga aaacaaaatt accttgattg gaatcaaaaa atctagttta
     1561 attacttaga ataaaatttc aataatattt ttatttacga aattaattta tgattattga
     1621 ttaattatcg gcaacaaaca tcaaacatcg aaaacatgga aaaaataatg tcaacaattt
     1681 ttgccaaatc gggcttggca tcagaaaaaa ataggtttat attcccacct acaaatttgt
     1741 tttcccatta gtacactatc aaccaaaagg agtatccgaa tgactgacct gaacaccctg
     1801 tttgccaacc tcaaacaacg caatcccaat caggagccgt tccatcaggc ggttgaagaa
     1861 gtcttcatga gtctcgatcc gtttttggca aaaaatccga aatacaccca gcaaagcctg
     1921 ctggaacgca tcgtcgaacc cgaacgcgtc gtcatgttcc gcgtaacctg gcaggacgat
     1981 aaagggcaag tccaagtcaa ccggggctac cgcgtgcaaa tgagttccgc catcggtcct
     2041 tacaaaggcg gcctgcgctt ccatccgacc gtcgatttgg gcgtattgaa attcctcgct
     2101 tttgaacaag tgttcaaaaa cgccttgacc accctgccta tgggcggcgg caaaggcggt
     2161 tccgacttcg accccaaagg caaatccgat gccgaagtaa tgcgcttctg ccaagccttt
     2221 atgaccgaac tctaccgcca catcggcgcg gacaccgacg ttccggccgg cgacatcggc
     2281 gtaggcggac gcgaaatcgg ctatctgttc ggacaataca aaaaaatccg caacgagttt
     2341 tcttccgtcc tgaccggcaa aggtttggaa tggggcggca gcctcatccg tcccgaagcg
     2401 accggctacg gctgcgtcta tttcgcccaa gcgatgctgc aaacccgcaa cgatagtttt
     2461 gaaggcaaac gcgtcctgat ttccggttcc ggcaacgtgg cgcaatacgc cgccgaaaaa
     2521 gccatccaac tgggcgcgaa agtgctgacc gtttccgact ccaacggctt cgtcctcttc
     2581 cccgacagcg gtatgaccga agcgcaactc gccgccttga tcgaattgaa agaagtccgc
     2641 cgcgaacgcg ttgccaccta cgccaaagag caaggtctgc aatactttga aaaccaaaaa
     2701 ccgtggggcg tcgccgccga aatcgccctg ccctgcgcga cccagaacga attggacgaa
     2761 gaagccgcca aaaccctgtt ggcaaacggc tgctacgtcg ttgccgaagg tgcgaatatg
     2821 ccgtcgactt tgggcgcggt cgagcaattt atcaaagccg gcatcctcta cgccccggga
     2881 aaagcctcca atgccggcgg cgtagcaact tcaggtttgg aaatgagcca aaacgccatc
     2941 cgcctgtctt ggactcgtga agaagtcgac caacgcctgt tcggcatcat gcaaagcatc
     3001 cacgaatcct gtctgaaata cggcaaagtc ggcgacacag taaactacgt caatggtgcg
     3061 aacattgccg gtttcgtcaa agttgccgat gcgatgctgg cgcaaggctt ctaagcaaac
     3121 gccgccgctc cgcaaacaaa atgccgtccg aaccgcaaat gctgttcaga cggcatttca
     3181 ttatccgccc gttcgaatcg ggtgatacta tttgtatcca gaattgttgg atagtttggg
     3241 agtggagagg aagaaatgat tatggaccaa tactatatgg agttaaagaa taagttatct
     3301 aacagaccaa tcctactgga taatactaat gattttcttt ttgttttagt aaatacagtg
     3361 aaagctatga tagaaaatac tgataaaagt cagctttcag agttagataa aattttagac
     3421 ggtgtcacaa gtcaagaatt gaaacttgcc tatgattttt gtcaagggaa attcggtcaa
     3481 gcaggttttt cttatcgtag gcacccgaat tatttttatt tgtccagttt aattgcgact
     3541 tttccagaat ttgagttgtc taaagcagac cgagattacc tcaaaggaat cataaatttt
     3601 gataactacc ttttatatga gttagattag tctttaatca cctcaaaacc ctatttattc
     3661 aaaaggagaa aaaatgattg agctgaaatt agtagatgag agcagttttc aggcagtgct
     3721 ggatttgaaa atatctgaag ctgatgaacg agcacgtttt gtggctccaa atgtacgctc
     3781 tttagctgac gcatggctct accgggaaaa tgaagatgtg tttccgaggg caatctattg
     3841 ggataagcaa gtggttggct ttctcctgct ggaaatagac aaggatgaag cagaatactt
     3901 tatctggcgg ataatgattg gtcag
//
LOCUS       DAWFAD010000160         3919 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWFAD010000160 DAWFAD010000000
VERSION     DAWFAD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.160"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            128..2713
                     /gene="acnB"
                     /locus_tag="WGO49_001814"
     CDS             128..2713
                     /gene="acnB"
                     /locus_tag="WGO49_001814"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ0306881.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHTL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFTSRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIADGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            2855..3850
                     /locus_tag="WGO49_001815"
     CDS             2855..3850
                     /locus_tag="WGO49_001815"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ0306882.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDG
                     IEYRGFAQETVEELTKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
BASE COUNT         1003 a         1140 c          991 g          785 t
ORIGIN      
        1 attacctgtc ataacagttc catttttgtt aaaactagtc tatgatagcg gtacaaatat
       61 tgtttacaat atttaacgca aatcatttgc aacccgacaa aagaaaaaca gaaaaaggaa
      121 caaagagatg ttagaagcct atcgtaaagc cgccgccgag cgcgccgccc tcggcattcc
      181 cgccctccct ttgaacgcgc agcaaaccgc cgatttggtt gagctgctga aaagcccgcc
      241 cgcaggcgaa ggcgagttct tggtcgaact gcttgcccac cgtgttccgc ccggtgtgga
      301 cgatgccgcc aaagtcaaag cctcattcct ggctgccgtt gccgaaggca gcgcgtccag
      361 cccgctgatc tcccccgaat atgcgaccga actcttaggt acgatgctcg gcggttacaa
      421 tatccacacc ttaatcgaac tcttggacga cgacaaactc gcgcccattg ccgccaaagg
      481 cttgaaacat acgcttctga tgttcgattc cttccacgac gttcaggaaa aagccgaaaa
      541 aggcaacaaa tacgcgcaag aagttttgca atcttgggca gatgccgaat ggtttacctc
      601 acgcgccaaa gttcccgaaa aaatcaccgt taccgttttc aaagttgacg gcgaaaccaa
      661 tacagacgac ctctcccccg cgcccgacgc gtggagtcgt cccgatattc cgctgcacgc
      721 gctggccatg ctgaaaaacc cgcgcgacgg catcacgccc gacaaaccgg gcgaagtcgg
      781 tccgattaaa ttgttggaag aactcaaagc caaaggccat ccggttgctt acgtcggcga
      841 cgtggtcggt actggttctt cacgcaaatc cgcgaccaac tccgtcattt ggcataccgg
      901 cgaagacatt ccgttcgtgc cgaacaaacg cttcggcggc gtgtgcttgg gcggcaaaat
      961 cgcgccgatt ttcttcaata cccaagaaga ctccggcgcg ctgccgattg aagtcgatgt
     1021 ttccgctcta aaaatgggcg atgtcgtcga tatcctgcct tatgaaggca aaatcgtgaa
     1081 aaacggcgag actgttgccg agtttgaatt gaaatcacaa gtattgctgg acgaagtgca
     1141 agccggcggc cgtatcaacc tgattatcgg ccgaggtctg accgccaaag cgcgcgaagc
     1201 cctgaaactg cctgcctcta ctgcattccg cctgccgcaa gcgcctgccg aaagcaaagc
     1261 cggtttcacc ttggcgcaaa aaatggtcgg ccgcgcctgc ggtcttcccg aaggacaagg
     1321 cgtgcgtccg ggtacttact gcgaaccgcg tatgacgacg gtcggctcgc aagacacgac
     1381 cggcccgatg acccgcgacg agttgaaaga cttggcttgt ttgggcttct ccgccgatat
     1441 ggtgatgcag tctttctgcc acaccgccgc ctatccgaaa cctgtcgatg taaaaaccca
     1501 taaagaactg cccgccttta tttccacccg tggcggcgtg tccctgcgtc cgggcgacgg
     1561 cgtcatccac tcgtggctca accgcctgct gctgcccgat accgtcggca ccggcggcga
     1621 cagccacacc cgtttcccca tcggtatttc cttccccgcc ggctctggct tggttgcctt
     1681 tgccgccgca acaggcgtaa tgccgctcga tatgcccgag tctgtattgg tacgcttcag
     1741 cggcaaactg caaccgggtg taaccctgcg cgatttggtg aacgccattc cgctgtacgc
     1801 aatcaaacaa ggtttgctga ccgttgccaa agccggtaag aaaaacatct tctccggccg
     1861 catcctcgaa atcgaaggcc tgcctgattt gaaagtggaa caagcctttg aattgaccga
     1921 cgcatccgcc gaacgctccg ccgccggctg taccgtgaag ctcaacaaag agccgattat
     1981 cgagtacatg aaatccaacg tcgtgttgat gaaaaacatg attgccgacg gctatcaaga
     2041 cccgcgtact ttggaacgcc gcatcaaagc catggaaaaa tggctggcaa atcccgagtt
     2101 gctcgaagcg gataaagatg ccgaatacgc cgccgtgatt gaaatcaaca tggacgacat
     2161 caaagagccg attatcgcct gcccgaacga cccggacgac gtgtgcttca tgtccgaacg
     2221 ctccggcacc aaaatcgacg aagtattcat cggttcgtgt atgaccaaca tcggccactt
     2281 ccgcgccgcc tccaaacttt tggaaggcaa ggcagacacc cccgtccgcc tgtggattgc
     2341 gccgccgacc aaaatggacg cgaaacaatt gtccgacgaa ggacactacg gcgtactcgg
     2401 acgtgccggc gcgcgtatgg aaatgccggg ttgctcctta tgtatgggta atcaggcgca
     2461 agtacgcgaa ggtgcgaccg ttatgtccac ctccacccgc aactttccga accgtttggg
     2521 taaaaacacc tttgtttacc tcggttcggc ggaattggca gcgatttgct ccaaactggg
     2581 taaaatcccg accgttgaag aatatcaagc caatatcggc atcatcaacg aacagggcga
     2641 taaaatctac cgctatatga acttcaacga aatcgacagc tacaacgaag tagccgagac
     2701 cgtgaacgtt taatccccgt catccgtatg aagtaaggga ttgaccgcaa tgccgtctga
     2761 acaaccttca gacggcattg caacattccg ctaacccttc tttccgcaaa cgctgcaaat
     2821 acggcgttca cgcccccaca taaaggaaac gacagtgaac ctgaaaaacc gccattttct
     2881 gaaactttta gacttcacgc cagaagaaat caccgcctac ctcgaccttg ccgccgaatt
     2941 gaaagccgcc aaaaaagcag ggcgcgagat tcagcggatg aaagggaaaa acatcgccct
     3001 gatttttgaa aaaacctcta ctcggacgcg ctgcgcgttt gaagtcgccg cgcgcgatca
     3061 aggcgcggga gtgacttatt tagagccgtc cgccagccaa atcgggcata aggaaagcat
     3121 caaagacacc gcccgcgtgt tgggcaggat gtacgacggc atcgaatatc gcggcttcgc
     3181 tcaggaaact gtcgaagaat tgacgaaata cgcgggcgta cccgtgttca acgggctgac
     3241 caacgagttc catcccacac aaatgcttgc cgacgcactg actatgcgcg aacacagcgg
     3301 caaacctttg aaccaaaccg cgtttgccta cgtcggcgac gcgcgttaca acatgggcaa
     3361 ttccctgctg attttagggg caaaattggg gatggacgtg cgtatcggcg caccgcaaag
     3421 cctgtggccg tctgaaggca ttattgccgc cgcacacgcc gccgccaaag aaaccggcgc
     3481 aaaaattacc ctgaccgaaa acgcgcatga agccgtgaag aatgttgatt ttattcatac
     3541 cgatgtgtgg gtcagcatgg gcgagccgaa agaagtctgg caggaacgca tcgatttgct
     3601 gaaagattac cgcgttacgc ccgaactgat ggcggcatcg ggcaatccgc aagtcaaatt
     3661 catgcactgc ctgcccgcct tccacaaccg cgaaaccaaa gtcggcgaat ggatttacga
     3721 aaccttcggg ctgaacggtg tggaagttac agaagaagta ttcgaaagcc ccgccagcat
     3781 cgtgttcgat caggcggaaa accgtatgca cacgattaaa gcggtaatgg tcgcggcttt
     3841 gggcgactga cagaactgtg cctgtttaaa ttcatccgca acacagatac cgtctgaaca
     3901 cgatgttcag acggtatcc
//
LOCUS       DAWFAD010000161         3898 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWFAD010000161 DAWFAD010000000
VERSION     DAWFAD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.161"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            362..2143
                     /locus_tag="WGO49_001816"
     CDS             362..2143
                     /locus_tag="WGO49_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ0306883.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDSALQKGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAENWLARHSDGLPAGIST
                     PQSSVSEADYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAQGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGTVCVPEPFKVSRFGSVWQMTSTIQAQALPHTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLEAEARGLYTGSIGYLNPCSGGLGFEGTFNV
                     VIRTLSLTPLSDGIYQGVYGVGSGIVIDSDPAAEYRECGWKARFLNELRPDFGIFETL
                     RVENGRCALLDRHLCRLKTSAQALNLPLPDGCENQIKQYIARLPDGAFRIKALLASDG
                     ISLSRAVLNRLTDKQRVIISPAVLPAQNYLRRFKTTCRALFDQAWQTAETQGAFDSLF
                     FNSDGILLEGGRSNVFVKHRGQWLTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETH
                     ITQKTLQEAEEIRLSNALRGVFAAALA"
     gene            complement(2202..3719)
                     /locus_tag="WGO49_001817"
     CDS             complement(2202..3719)
                     /locus_tag="WGO49_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0306884.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAEKLDLPAPVLENVGRFRKKAEGLTGWRFSGGISPAVNRNANNAAPQYCRQNGGS
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
BASE COUNT          932 a         1278 c          871 g          817 t
ORIGIN      
        1 caacgaagcc gaagacagta cagatagtac ggaaccgatt cacttggtgc ttcagcacct
       61 gagagaatcg ttctctttga gctaaagcga ggcaacgctg tactggtttt tgttaatcca
      121 ctatacttgg agctggtctt gcttttcgcc taattctacg ttttcaaacg gttgcagctg
      181 gtggtctgtc ataaaggtct ccttattgta tttcaggttg gaaatcggaa tttgttttca
      241 caattttaca ccttcgcccc ccactttctc tacataaaat tacattttgc cgatatttgc
      301 cgaatcgtct gaaaatatgt gtaaaaagag cgtataatca aaacatttgc cccgaattgc
      361 catgccttat ttcgccctgt ttgacgatgc cgtaagcggc cgcgcaaaac gctatcaaaa
      421 tcatgtggaa agccgttttt tccgtcccga agaactcgat gctttggaca gcgcgctgca
      481 aaagggctgg caaaaagggc tgcattcggt gttgtttgca gactacggat tcggtttgcc
      541 gctgacgggg gtcgagtccg aacgcggcgg caaccttgcc ctgcactggt ttgccgactg
      601 cgccgacacc gatgccgaaa actggcttgc ccgacattca gacggcctcc ccgccggcat
      661 ttccacgccg caatcctccg tatccgaagc cgattacctc gaccatatcc gccaaatcca
      721 cgaagccatc cggcgcggcg acacctatca aatcaactac accacccgcc tgcacctgca
      781 agcctacggc aatcccgtca gcctctaccg ccgcctgcgc cagcccgtcc cctatgccgt
      841 tttgtcccac ctgcccgatg cacaagggca atccgcgtgg acgctgtgtt tctcgcccga
      901 actcttcctc aaaatcggtt cggacggcac catcagcacc gaaccgatga aaggcaccgc
      961 gccgattttg ggcgacggac aagacgaacg ccgcgccgcc gagttgcaag cagacccgaa
     1021 aaaccgcgcc gaaaacgtga tgattgtcga tttgctgcgt aacgacctcg gcaaaatcgc
     1081 ccaaaccggc acagtatgcg tacccgaacc gtttaaagta tcgcgtttcg gcagcgtttg
     1141 gcagatgacc agcaccatcc aagcccaagc cttgccgcac acctcgttcg ccgacatcct
     1201 ccgcgccgcc ttcccctgcg gcagcatcac cggcgcgccc aaaaaaatga gtatgcagat
     1261 tatcgaatcg ctcgaagccg aagcgcgcgg actttatacg ggcagcatcg gctatttgaa
     1321 cccgtgttcc ggcggcttgg ggtttgaagg cacgttcaac gtcgttatcc gcaccttgtc
     1381 gctcacgccg ctttcagacg gcatttatca aggcgtgtac ggtgtcggtt ccggcatcgt
     1441 catcgacagc gaccctgccg ccgaatatcg ggaatgcggc tggaaagccc gtttcctcaa
     1501 cgaattgcgc cccgacttcg gcatttttga aaccctgcgc gtggaaaacg gacgctgcgc
     1561 cctgctcgac cgccacctat gccgtctgaa aacctccgcc caagccctca acctgcccct
     1621 gcccgacggt tgcgaaaatc aaatcaaaca atacatcgca cgcttgcccg acggcgcgtt
     1681 ccgcatcaaa gccctgctcg cttcagacgg catcagcctg tcccgcgccg ttttaaaccg
     1741 tctgaccgac aaacaacgcg tcatcatttc gcccgccgtc ctgcccgcac aaaactacct
     1801 gcgccgcttc aaaaccacct gccgcgccct cttcgaccaa gcgtggcaaa ccgccgaaac
     1861 acaaggcgcg ttcgacagcc tgtttttcaa ttcagacggc atcctgctcg aaggcggcag
     1921 aagcaacgtg ttcgtcaaac atcgcggaca atggctcacg ccctccttag atttagacat
     1981 tttaaacggc ataatgcgcc aagccgtatt ggacgaaccg caaaaatatt tgcaaacaaa
     2041 tcaagtaatc gaaacacaca tcacacaaaa aacactgcaa gaagccgaag aaatccgcct
     2101 ctccaacgcc ttgcgcggcg tatttgccgc cgcccttgcc tgaacgcgca aaaatgccgt
     2161 ccgaacctgt ttccaaagtt cggacggcat tatcccacca ttcaaaaccg ccaatccgcc
     2221 gacacaaaca cctcgctgtt gcggcgtttc gcatacggca cattactttc cgtcctgccg
     2281 aaacgataat tcaacgccgg cacgatacct ttgtacgaca acttgtcgtg gctcaaagcc
     2341 agcgagacat tccattcgcg gttgcgttgc gcctctgtcg agaaagccgc aatgccctta
     2401 tagttgcggc gggcataaga cgcagaaacc cgactgttca aaccgcccaa ctgccgccac
     2461 tcctgcgccc aaccggcata aacaccgttg cgccggtagg cggcattatt gaccgcgccg
     2521 cccaccgttt cgcgtttcgg cacaaaccgc acaaactgcc agccgccgaa cacagttgcc
     2581 gattcgccca aacgttttgc cgacgaaaca taaaacccgt cctgcctgcc gttattgtat
     2641 tccgccctat cctgttcgcg gtagcgttgg cggtaatgtt ccagcgcgac cgaaaattgc
     2701 catcccgggt ttgggcggta agtatgggac aactgcacgc cgactccgtg cgccagcata
     2761 tacggcggca ggcggcggtt gtttacccgt tttgttttcg catcaaagcc gtcgctgccc
     2821 gacaactgca cctgataaaa cggcaaaatc cccgccgtct gccgtgcatt tttatactgc
     2881 caacccaaat acgctctgcc gaacccgtcg tcataagctg attttttact gaaataatag
     2941 ctcgtgccgc cgatattgga acggaacaac aaataatgat tatctgccaa cgccgtcagt
     3001 ttttccgcct cgatttcata attcaagcct gccgcccgct ccgcccggct gacactgcat
     3061 atctgactgc ctccgttttg ccggcaatac tgcggcgcgg cattattggc atttctattg
     3121 accgccgggc tgataccgcc cgaaaaacgc cagcccgtca gcccctccgc ttttttccga
     3181 aaacgcccca cattttccaa aaccggcgcc ggcaaatcca atttttccgc ctcggcaaaa
     3241 tgcctttctg ccgacttcag ccggaaatcg tcaaactccg ccgccgccaa atccagcaaa
     3301 atccgctcgt ctgccgcatt ttccccgtgc agttcccgat accgcgccac cgcctccgcc
     3361 ggccttcccg ccaatttcgc cagcaaagcc cgcgccctgc cgtacaaaac cgcgtcataa
     3421 tccggcagct tggcatacaa atccgccaac gaagcgatta aatccgcctg attgccgttg
     3481 agcgcgtcgc gcaaactatg ttccaacatt ttcgggtgcg ccaacaaaaa atccccgtca
     3541 accacgcgcg gggcatcatt ttcaactttc caatccgatt ccgcccactt atccgacacc
     3601 gaccgctgca cctgcaacaa tgccttgtca tccaaaatcg cgggcgcatc cgccccatag
     3661 gcggcagaaa cacctgccgc acaccaaaca accaaaaaac cgtatctgaa atacaacata
     3721 ccctgtcatt tacctttctg gcaaacacgc cgccgaagca cgtcaaacca tccgaaaaac
     3781 aggcagaaac ccgtgaaaac cggctttgcc gtctgaaagc gggcaggcaa aaaaccgccg
     3841 cccgattttc aaagggcgga tttcacattt atagtggatt aacaaaaatc aggacaag
//
LOCUS       DAWFAD010000162         3888 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWFAD010000162 DAWFAD010000000
VERSION     DAWFAD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.162"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..2121
                     /locus_tag="WGO49_001818"
     CDS             <1..2121
                     /locus_tag="WGO49_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0306885.1"
                     /translation="GKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFGG
                     ESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTASVQVGSAQLIKGASTAVNGMNPE
                     GAVSGSVNIETKKAADEGNRKIGLGRFSNNRAQGTFDLGQRFGENKAFGVRANGKLRH
                     GDTPRHGYSEDNKEFAVNADYRGETLRVAFDSIYAKRKTNGGRARMQDIQNAGGRLFG
                     APDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVCL
                     NATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLSARGEFETGPVTHN
                     WSTAFDRVIRQRKTINGSGNGNSKIEVKANENIANQLASFTADYPNSWENSANLDANI
                     KVNSLALSDTLGFVDNKYRLTLGGRFQAVEYTNKKKSQSGDAKRFSPMLMAAWVPQPD
                     LVVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKRP
                     GYWRGHTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANLL
                     NNTLRPTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLNG
                     NVSYFGKSYQDTQKQYAFPSYTLVDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQR
                     GQYDRSFAVVGMPRTYWLKAELDF"
     gene            2245..3795
                     /locus_tag="WGO49_001819"
     CDS             2245..3795
                     /locus_tag="WGO49_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ0306886.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGNEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTQPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEA
                     GMVSEPQQ"
BASE COUNT         1100 a          955 c          980 g          853 t
ORIGIN      
        1 ggcaaagcca acgcctttac cgcgccgatt accgtcgtca actacgacga acaagccctc
       61 aacaacaccg aagcgcgtac tttggtggat gccgtagcga aaaaagacgc ttccgtttgg
      121 cagttcggcg gagaaagcaa cacgctgact ggactgtatt tcagaggtta tcagcttgat
      181 gcgcgccaat tcagcgtcaa cggtttggca ggtatgtacg gcacgcaagg cacggccagc
      241 gtgcaagtcg gctccgcaca actgattaaa ggcgcgtcca ccgccgtaaa cggcatgaac
      301 cctgaaggtg cggtatccgg ttccgtcaat atcgaaacca aaaaagccgc cgatgaaggc
      361 aaccgcaaaa tcggtttggg ccggtttagc aacaaccgcg cccaaggtac gttcgatttg
      421 ggtcaacgct tcggcgaaaa caaagcattc ggcgtacgcg ccaacggcaa actgcgccac
      481 ggcgacactc cgcgccacgg ttacagcgaa gacaacaaag aatttgcggt aaatgccgac
      541 tatcgcggcg aaacactgcg cgtagcgttc gattccatct acgcaaaacg caaaaccaac
      601 ggcggtcgcg cgcgtatgca ggacattcaa aacgccggcg gacgcttgtt tggcgcgccc
      661 gatggtaaaa ccaacctgct gccttcttgg aactggcaaa ataccgccgg ccaaaccaat
      721 atgcttacgt ttgaatggga tgcgtttgat aatgcacaga ttacaggcgg tatcggctac
      781 aacaaggcac gctattacgg cacgctgatt tccccgaccg tatgtttgaa tgctaccagc
      841 acttgtaccg atatgcaaaa cacaaacgcc agcggcaaag taacggcacg caactatgac
      901 tacaataccg gtactgcgcg cctgaccgac caatacttcc gcactttaag catgaacctg
      961 tctgcgcgag gcgaatttga aaccggcccg gtaacacata actggagtac ggctttcgac
     1021 cgcgttatcc gccagcggaa aaccataaac ggcagcggga acggcaacag caagatagaa
     1081 gtgaaggcaa acgaaaatat tgcgaatcaa ttggcatcgt ttacagccga ctatcccaat
     1141 tcttgggaga actctgccaa cttggatgcc aatatcaaag tcaacagcct ggctttgtcc
     1201 gacaccttgg gcttcgtgga taacaaatac cgcctgactt tgggcggacg tttccaagcg
     1261 gttgaataca ccaataagaa aaaatcacag agcggcgatg ccaaacgctt cagcccgatg
     1321 ctgatggcgg cgtgggtgcc gcaacctgat ttggttgtgt acggcaacta tatggaagac
     1381 ttggagcctg ccgatatcaa aaccgatgat tccggcgaaa cgacaatggc gaaaccgcgt
     1441 gtcagccgtc agtttgaagt gggcgtgcgt aaaaactggg gcaatttcgt aaccacgtta
     1501 aacgcgttcc aaatcaaacg cccgggctac tggcgcggtc atacaactac caagacaaca
     1561 aaaggggtaa caacaacgct gacctatggc aacaattccg atttcgcccg ctataaggcg
     1621 caaggcggcg cggccggcga cgagcaaggt atggaacgca atcgcggtat tgagttcaac
     1681 acctacgcga acttgctcaa caatactttg cgtccgactt tgggtctgat gtatctgcaa
     1741 tcgaccgtga aagagtatcc aaattcacgc gatatgctgg ttaacggcgt acaagtcgcc
     1801 aatccgcgcg taattgccaa agcaggtttg gaatgggata caccgtttgc caaaggattg
     1861 actttaaacg gtaatgtttc gtatttcggc aagtcttacc aagacacgca aaaacaatac
     1921 gctttcccgt cttacacttt ggttgacgta ggcgcgcgct acaaaaccaa gctcggcaaa
     1981 aatacgttga ccgtcagcag ctcggtggaa aacctgttca acaaaaacta ctggcaggta
     2041 cagcgcggcc aatacgaccg cagcttcgcc gtcgtcggta tgccgcgtac ttactggttg
     2101 aaagcggaat tggatttctg atttctccgc atccggaaaa tgccgtccga acaggtttca
     2161 gacggcattt cttttaaaga aatatggcgg gatatgctta aataatgcta ttctagcgtc
     2221 tttgaaacct gcttgtttga agtgatgaaa aaacctttga tttcggttgc ggcagcattg
     2281 ctcggcgttg ctttgggtac accttattat ttgggtgtca aagccgaaga aagcctgacg
     2341 cagcagcaaa aaatattgca ggaaacgggc ttcttgaccg tcgaatcgca ccaatatgag
     2401 cgcggctggt ttacctctat ggaaacgacg gtcatccgtc tgaaacccga gttgctgaat
     2461 aatgcccgaa aatacctgcc ggataacctg aaaacagtgt tggaacagcc ggttacgctg
     2521 gttaaccata tcacgcacgg cccttttgcc ggcggatttg gcacgcaggc gcacattgaa
     2581 accgagttca aatacgcgcc tgaaacggaa aaagttttgg aacgcttttt tgggaaacaa
     2641 gctccggttt cccttgccaa taccgtttat tttaacggca gcggtaaaat ggaagtcagt
     2701 gttcccgcct tcgattatga agagctgtcg ggcatcaggc tgcattggga aggattgacg
     2761 ggaaaaacgg tttatcaaaa aggtttcaaa agctaccgca acagctatga tgcgcccttg
     2821 ttcaaaatca agctggcaga caaaggcgat gccgcgtttg aaaaagcgca tttcgattcg
     2881 gaaacttcag acggtatcaa tccgcttgct ttgggcagca gcaatctgac cttggaaaaa
     2941 ttctccctag aatggaaaga gggtgtcgat tacaacgtca agttaaacga actggtcaat
     3001 cttgttaccg atttgcagat tggcgcgttt atcaatccca acggcagcat cgcaccttcc
     3061 aaaatcgaag tcggcaaact ggctttttca accaagaccg gggaatcagg ctcatttatc
     3121 aacagtgaag ggcagttccg ttttgacacg ctggtgtacg gcaatgaaaa atacggcccg
     3181 ctggacatcc atatcgccgc cgaacatctc gatgcttctg ccttaaccgt attgaaacgt
     3241 aagtttgcac aaatttccgc caaaaaaatg accgaggaac aaatccgcaa tgatttgatt
     3301 gccgccgtca aaggcgaggc ttccggactg ttcaccaaca atcccgtatt ggacattaaa
     3361 acttttcgtt tcacacagcc gtcgggaaaa atcgatgtgg gtggaaaaat catgtttaaa
     3421 gacatgaaga aggaagattt gaaccaattg ggtttgatgc tgaagaaaac cgaggcagac
     3481 atcagaatga gtattcctca aaaaatgttg gaagacttgg cggtcagtca ggcaggcaat
     3541 attttcagcg tcaatgccga agatgaggcg gaaggcaggg caagtcttga cgacatcaac
     3601 gagaccttgc gcctgatggt ggacagtacg gttcagagta tggcaaggga aaaatatctg
     3661 actttgaacg gcgaccagat tgatactgcc atttctctga aaaacaatca gttgaaattg
     3721 aacggtaaaa cgttgcaaaa cgaaccggag ccggattttg atgaagccgg tatggtttca
     3781 gagccgcagc agtaatgggt caaatgccgt ctgaaatgtt caggcggcat tttgttgtat
     3841 tggaaactgc cgttttcaac cattccttta gataagccgt gtcggatt
//
LOCUS       DAWFAD010000163         3861 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWFAD010000163 DAWFAD010000000
VERSION     DAWFAD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.163"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="WGO49_001820"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="WGO49_001820"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ0306887.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLERFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGLAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDADGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="WGO49_001821"
     CDS             1439..1924
                     /locus_tag="WGO49_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ0306888.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGO49_001822"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="WGO49_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ0306889.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          786 a          943 c         1076 g         1056 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cttcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 ccgtcagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataag
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgccga tgtcaaacct
      661 gaggacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggcgcg
      721 ataccgcgtt gggtgttgtt aagtgcggct ttgcccgaat tggatgaggt atggctggaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcattgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcctcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaggttttag atgccgacgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgt gttaccggtt tatatttaat ccgtttcggt ttcgcgcctt cttcgccgag
     2041 tcggcgtttc ttatcggctt cgtattcctg atagttgccg tcaaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccagc ggtcgtgcga
     2161 aatcaccatc acgctgccgg caaattccag caatgcgtct tccaacgcgc gcagggtttc
     2221 cacgtcgagg tcgttggacg gttcgtccag cagcaacaca ttgccaccgc ccaacaaggt
     2281 ttttgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcgtcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttaaacagt gtcgatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttgtcg ccgaacgatt tggaaacatt cacaaattca atcacttcgt tacccaaacg
     2701 ctcggcgacg ggaatgaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcttca aaacgagcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcattgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgtgttacc gcaacgactg taccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 agattccgcg tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gtttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc ggcgatttcc aattcgtgtt ccgcaccgcc
     3241 gcccgtggac gaacctgccg caataatcgc ttccaagcgg ccctgctctt ctgccaacgc
     3301 gtcaaaatcc gcatcaggat tggcgtactc ggcatacact tcttccaaac gtttctgcgc
     3361 ggcagccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt aatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacacccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taatgatggt
     3601 tttctgcggc ggcacaacct tgcttacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgccta
     3781 tccctttgaa attatacaga caaaaaaaat aataatgata ggagatcgcc gcaccggcaa
     3841 ccatttcgga ttttccaaag c
//
LOCUS       DAWFAD010000164         3855 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWFAD010000164 DAWFAD010000000
VERSION     DAWFAD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.164"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(88..906)
                     /locus_tag="WGO49_001823"
     CDS             complement(88..906)
                     /locus_tag="WGO49_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0306890.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTGLSDRLPLSVLTGSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(973..2223)
                     /locus_tag="WGO49_001824"
     CDS             complement(973..2223)
                     /locus_tag="WGO49_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0306891.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAVRIWPS
                     HTYTRLTLESTTALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRNIR
                     AGQNTPDTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="WGO49_001825"
     CDS             complement(2220..2681)
                     /gene="tsaE"
                     /locus_tag="WGO49_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ0306892.1"
                     /translation="MSDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            2871..3683
                     /locus_tag="WGO49_001826"
     CDS             2871..3683
                     /locus_tag="WGO49_001826"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ0306893.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRNNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAHNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT          850 a          966 c         1137 g          902 t
ORIGIN      
        1 cactataacc gaaggtaagg gaggggtgtg tccactgtgc cgccgcattt gccgcgtgcg
       61 gaagatacgg ataacaaccc cttggaatta aagggagaag ttgcgggaaa tatccatatc
      121 aggctgggga ccgtacttat ttacggtatt ttcgtatgcc gaaataaaat atttcttgaa
      181 taaaccgtaa tcgccggttt cgtaataggc tacgacagcc tcggcatagg cgggataact
      241 gtcgggggag aatacgcaag gaaaaaaacc ggagcgcatc agcgacaagg tcatgcagtt
      301 tctggcagta cgtttattgc aatctttaaa atattgaaga tacgccaaat tattatggag
      361 ataaacggcg cggtcgaaag gattttctat tttgggagct tcttgaagca accattttaa
      421 ttccgtatcc aaactttgcg ggttagataa aggggtgtag tctgtcccgc ttatggtaac
      481 gctgtcgcgg cggactacgc cgccggagcc tttttccagt aagttttcgg aaatcaggct
      541 gtgggtggtt tttaagaaat ccaaccagtc aaacggctcg gggctgtcca aaccggacag
      601 caggtgccgg taactttccc tcaggttgat taacattacg gcatccgaat ataacttccc
      661 gcctgcggtt tgcccgagtt taagcaatgc ctgcgtatcg tattgattat aagtgtttcc
      721 ttccaatttg gcagaactat atacaaaatc gataccgatt tcttcaatat tacgcacgga
      781 gcccgttaaa acagataaag gaagccgatc ggacaatccg gtcaggtatt cttgttcgcc
      841 ggctgtaaaa atggggcgga tatttaaaaa atcctgttgg tatctcaatt tatccatacc
      901 tttcatcata aaaacgcggt ttggggcgga agtaggttta tgcctgcctt catccgacta
      961 ccgccttttc aatcaacccc gcttcaatac ggatgtattg atgtagcgtt ggacacccga
     1021 ggcaatggat tgggcgcact gccggcggaa ggattcgctg cccagcaact tctcttcagc
     1081 agggttggac aggaaggcgg tttcgaccag gatagacggc atatcgggtg cgcgcaaaac
     1141 ggcgaaattg gcttcgtcca ccctgccttt gtgcaagtgg ttgagcctgc ccaattcttc
     1201 aagcaccagt ttgccgagtt tgcggctgtc gcgcagcgtg gcggtttggg tcatatcgag
     1261 cagggcggta tcgacgttgc ggttgccgct ggtcggtacg ccgccgaccg cgtcggcatt
     1321 gttttgcgtc tgttccaaga atttggcggc ggagctggtc gcgcctttgg tgttcagcat
     1381 atagaccccc gttccgcgcg cggaggggct ggtgaaagcg tcggcgtgga tggagacgaa
     1441 tacgtccgcc cgccgtgctc gccctttggc gacacgcacg cccaatggga tgaacacgtc
     1501 ttcgttgcgc gtcataaaga cgttgtaacc caaggcttcc aattgttttt tggtttcgcg
     1561 ggcgatggag aggacgacgt gtttttcctg caaaccgccc gggctgacgg cgccggggtc
     1621 ttcgccgccg tgtccggggt cgatcataat gacgggtctg cgcccgtttt tgccgcgttc
     1681 ggtttgtgcg ggagtgtttt gagcgaggtc ggcttcaggg gagccgcgac gggttttgtt
     1741 caggctgccg ttaagcagcg ccatcatcgg atcgtcggga tccatccctt gcggatagag
     1801 atcgacgacg aggcggtttt taaagccgcc gacggggggg agggaaaaaa cttgcgcgta
     1861 ggaaggctgt ttcaaatcga tgacgaggcg gacggtgtcg ggcgtgttct gaccggcgcg
     1921 tatattgcgg ataaaggggt cgtccgccat aaccttctga gacagtccgt gcaatacggt
     1981 attgatgttc gcgttttgta tgtcgacgac caacctgccc gggttgtcga gcgtgaagtg
     2041 ttggtatttg agcgcggttg tgctttccag cgtcaggcgg gtgtaggtgt gcgacggcca
     2101 tatccgtacg gcggtaaact gcggggcgcg taccgttttg gcaacggcgg atgcgatggg
     2161 gcttagggcg aacagtgtgc cggcggtgcg gcggatgatt tgtcttcgtg tcagtttggt
     2221 catagcggca ggctttcgcg tcctcgttcg gtatgggcgg tcagcaggca ttttctgccg
     2281 ccgccgtcgt gtgtcaatgt tgcggtgatg tcggcgggcg gggtaaattc gcctccctgt
     2341 tgcggccatt cgatcaggca gacgctgttt gcggcaaaca gttcgtcaag ccccgcgtct
     2401 tcccattctt cggggaacga gaagcggtag aggtcgaaat ggtgcagggt gaagcgttcc
     2461 agcggataag attcgacgat ggcgtaggtc gggcttttga ctgcgccctg atgacccaat
     2521 ccgcgcagga tgccgcgtgt cagcgtggtt ttgcccgcac ccaaatcccc ttcgagataa
     2581 atgaccagcg gtgcgtttaa acgggaagac cacgccgcac ccaaatcgag tgtggcggct
     2641 tcgtcggcaa ggaatcggga aacgggagga aaatcagaca taggggcttt acaactttta
     2701 tgtggcggat cggaggggca ggcggtcggc aaaccgtgcg cccggcggca gcattgccgt
     2761 acattataac agccaaatcc gcatcctgct ttcagacggc aacggctgtc aagaaaaagc
     2821 ggcgcgtgta caatacgcgg attgtatgtt taggacggat tggaaaaaga atggaaaata
     2881 tcggcaggca gcgacccatc ggcgtttttg actcgggaat cggcggtttg accaatgtgc
     2941 gagcgctgat ggaacggctg ccgatggaga acatcattta tttcggcgac acggcgcgcg
     3001 tgccttacgg gacgaaatct aaggcgacca tcgaaaattt ctcgatgcag attgtcgatt
     3061 ttttattgga acacgatgtc aaggcgatgg ttatcgcgtg caatacgatt gcggcggtgg
     3121 cggggcagaa aatccgtcaa aaaaccggca atatgcccgt tttggacgtg atttccgccg
     3181 gcgcgaaagc cgcgctggca acgacgcgca acaataaaat cggcattatc gccaccaata
     3241 cgacagtcaa cagcaacgcc tatgcgcgcg ccatccacag gaacaacccc gatacgctcg
     3301 tccgcacgca ggccgcgccg ctgctcgtcc ctttggtgga ggaaggttgg ttggaacatg
     3361 aagttacccg cctgaccgta tgcgaatacc tcaaaccatt gcttgcggac ggcatcgata
     3421 cgctggtgtt gggctgcacg cactttccct tgctcaaacc cttaatcggc agggaggcgc
     3481 acaatgtcgc actggtcgat tccgcgatta cgactgccga agaaaccgca cgcgtccttg
     3541 cgcaggaggg attactcaat accgacaaca acaatcccga ctaccgtttt tatgtcagcg
     3601 atattccttt gaaattcaga accatcggcg agcgttttct gggcaggacg atggagcaga
     3661 ttgaaatggt gtctttgggt tagcggcagg tacggacgga aaccgtcccc gtccgcgcca
     3721 tccgcatacg cacccgcaac gccctgccgt attaaacgcc ccgtcgccgc aaatgccgtc
     3781 tgaaaggctt cagacggcat tttttacaca attcccaccg tttcccatcc ttctcgatac
     3841 actgtaatct cgaaa
//
LOCUS       DAWFAD010000165         3852 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWFAD010000165 DAWFAD010000000
VERSION     DAWFAD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.165"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(267..2699)
                     /gene="hupB"
                     /locus_tag="WGO49_001827"
     CDS             complement(267..2699)
                     /gene="hupB"
                     /locus_tag="WGO49_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HEZ0306894.1"
                     /translation="MHIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGDVEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTDTTTSAATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDLRGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYAPKILASNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPELKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDNPNKPTYAPLSDGTALVSSP
                     VWQNQNRTAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINAL
                     SPWTAVYSLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(2730..3758)
                     /locus_tag="WGO49_001828"
     CDS             complement(2730..3758)
                     /locus_tag="WGO49_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein"
                     /protein_id="HEZ0306895.1"
                     /translation="MKYKALSLLPLAAALAACAGGGVAEPHIPVSIPTATPLPTGEVK
                     LSDDNSKIENINTAGTGSTSGISIQQREVEKEPFPGYKTKETSFIFQTPGGAQYALSS
                     YADPITVSYSSPDFKIPDRHAGQRLADGSRIFICCSESGATSYAEITKQDYMKFGAWI
                     GPNGEIDLFAGGFPVGKTPKPAYSWSDDTPETAGKGKITYQVWGIRVKDGQFVTSSYT
                     PPKNSGYTFNPTNTPVLSFITANFNSNKLAGKIIGNSDYGPDVEIKEAQIDGLSFSGD
                     ATSGGKTGKLEGKFFGKFNSSYDSDTSIGGKITFDGDRSLDTVFGGVSYKKELESTTD
                     RETTHLTK"
BASE COUNT          705 a          894 c         1206 g         1047 t
ORIGIN      
        1 ggtgcaggtt tctgtgcgga tagcttcgtc attcccgcgc aggcgggaat ccaggttcgt
       61 tgagtttcag tcatttccga taaattcttg cagctttgcg tttctagatt cccacttccg
      121 tgggaatgac ggagagtcgc gggaatgacg gagagttgcg ggattgggct tggtacaagc
      181 ctgaaagggg gcggcggtgc gaccgtaaaa aatgccgtct gaaacccaaa tcatcgggtt
      241 tcagacggca ttggcacatc aggagcttag aacttcgctt cgatggtgaa attgtagctc
      301 ctgcccggcg aggtaaagcg ttggatgccg gcgtgggttt tgttgtcaac gcggttgacc
      361 gtgccgaact cgcggatgct gcgtaaagat tcccaagtgt agtactgctt gttggtaatg
      421 ttgtacgcgg cggcgcggag cgtaacctgt ttgccgatgt tgaggtaggc ggaaaggtcg
      481 aacagcgtat aggccttgct gtgtttggca taaggccacg ggttgttcaa gtcgtcgttg
      541 ctgtggacgg tgtcggacgg ctttttggcg gcggtgcgcg tggcgtaggc gttgatgccc
      601 caacgtttgg aaggagcgtc gtagcccagg ctgtaaaccg ccgtccacgg cgaaagcgcg
      661 ttgatgggcg tttctttgcc gttgttttgc gttgccttgc ccttgatgta gctgacgttg
      721 aggccggtgt gcagcccttt gggcagcccg atgctgtcga ggttccaagt gccgttaaac
      781 tctatgcctt ttacccaggc ggcggtgcgg ttttggtttt gccaaacggg cgagctgacc
      841 aatgccgtac cgtctgaaag cggggcgtag gtgggcttgt tagggttgtc tgacgaaacg
      901 cccatgtagg tcagttcgat aaagtcgcgg tatttggttt tgaagcccga gagcttgaag
      961 ttgcccgctt tgccgctgcc cgccaggccc aattcccagt ttttggcttt ttcggctttc
     1021 agttcggggt tggctttcag gtagaaatcg gggtgtggga acagcagcca ggtttcgtcc
     1081 gaagtcggcg cgcggaagcc ggtgctgtat tttgccaaca agtgcagatg cttggtaaac
     1141 cgccagtcga accccgtgcc gtagctgaag cccgcgtgcg cgcgttccga acccaaatgg
     1201 ggaatctgcc cccggatggc ggtggtgtat ttcggatcgt ctttcgcgct gctgctgttt
     1261 ttgtcgtagc gtatgcccgc attcaggcgg aagcggtcgt tgccgcccaa gtgaaacgca
     1321 ttgttccaat aggcaaattt atatttcgac cggttttcga tcagcattgt gattttggct
     1381 tggttggaag cgaggatttt gggggcgtac agttttgcga aatagctgta atccgagttg
     1441 gcattgtccc ctctgccgcc gcccaagccg tattgcgccg cccaaacggc tttggaaaag
     1501 tcgagttgtt tttcaaaatc ggcagtccat tgcgcggtgt tttggcggat atgccggaac
     1561 gaatggtaaa cctcgccgtt gatgccgcgc aaatcgtaat ttttcgggat gtcccaagtc
     1621 caagtgttca tatcgatgcg ctgcttgtcg tagcgcagct tgaggctgtc ccacgggccg
     1681 tgttccaatt cgtttttgta ttcgacaccg gagcggcggc ggtagctcac gtcttggcgg
     1741 tggcggtagt cgcccgttgc ggcagacgtg gtcgtatccg tccacaaatt agacagctcg
     1801 ttggtaaaac ggtcggtgcg cgagtcttca aaaatccagc cgatacggtt ttgatcgttg
     1861 aagttgtagc ccagcttgaa cagggtgctt ttgtttaccc attcctgtgg gtcgggttgg
     1921 gagcgcgcaa cccctgtggc tacataggtc aggtacttgc tgggtccgcc tgtatcggtc
     1981 gggttataga catatccgtc gtttttaatt tcgacatcgc cctcagtcga gcggtttttg
     2041 gtttctttgc cgaagcggcg ggtataaacc aataaggcat ccaaaccaaa gagcctgccg
     2101 gcggcggtga tgctgctgaa tttttggctg tttttgccga cgctgccgcc ctttatcccc
     2161 aaatggtagg gcttgtcttc ggaaacataa tcgcttgcgg atttggtttg gtaattgact
     2221 gcgccgccca atgcgccgct gccggatttg agcgagtccg cgcctttggt gatggttact
     2281 tcggaaaagt tttccggctc ggaagtgttg cggttggcgt tgaagttgcc gtacgcgccg
     2341 aacaattctt ggaaagcttc ggaagagcgg ctttccgcct gcgccagccc gtcaacgttg
     2401 atggcgacgc ggtctttgtc cacgccgcgt atggtaaagc cgttagaacc cgccctgccg
     2461 ccttcgacga cggaaatgcc ggggtcgtag cgcaccaggt cgtgttcgtc gttgaccaag
     2521 agcttgtcta aggttttgcg gcggattttt tcttcgccga gtttttgggt tttgtgcgtg
     2581 ccggtaacgg tgatttcgtt cagcgtttgg gcggactgcg gcgcggggtc tgccgcaaag
     2641 gcgggaaacg cttgggcgat ggcggcggca gccaatacgg gtttgaaggg gatgtgcata
     2701 agaaaacctt tttaaaagcg gattaattat tattttgtga gatgggtggt ctctcgatcg
     2761 gtggtgcttt caagttcttt tttataactc acgccgccga atacggtatc gagggagcgg
     2821 tcgccgtcaa aggtgatttt gccgcctatg ctggtgtcgc tgtcatagct gctgttgaat
     2881 ttgccaaaaa acttgccttc caatttgccg gttttgccgc cggaggtggc atcgccggaa
     2941 aaggataatc cgtcaatctg tgcttcttta atttccacat ccggcccgta atcgctgttg
     3001 ccgatgattt tgcctgccag tttgttactg ttgaaattgg cggtgataaa ggaaaggacg
     3061 ggggtgttgg taggattgaa cgtataacca ctgtttttcg gcggcgtata agaggaagta
     3121 acaaattgcc cgtctttgac gcggatgccc caaacctgat aagtgatttt gcccttgccg
     3181 gcggtttcgg gcgtgtcgtc gctccaggag tatgccggtt tcggcgtttt gccgacgggg
     3241 aagccgccgg cgaagaggtc gatttcgccg ttgggaccta tccacgcccc gaatttcata
     3301 taatcttgtt tggtgatttc cgcatacgaa gtagccccgg attcgctgca acagataaag
     3361 atgcggctgc cgtcggcaag ccgctgccct gcgtggcggt cgggaatttt gaaatcgggg
     3421 gaggagtagc tgacggttat ggggtcggcg taggatgaaa gggcgtattg cgcgccgccg
     3481 ggtgtttgga agatgaatga tgtttctttg gttttgtagc cggggaaagg ttctttttct
     3541 acttcccgct gctgaatgga aataccggat gtgcttcctg tgccggcggt gttgatgttt
     3601 tcgattttgc tattgtcgtc tgataacttt acctcgccgg tgggcagcgg ggtggcggtg
     3661 gggatggaca cggggatgtg cggttcggct accccccccc ctgcacaggc ggcaagggcg
     3721 gcggcaagcg gcagtaagga cagggctttg tatttcatcg catactcaat cttgatgatg
     3781 tgttaataat agttttgatt atcagtttaa atgttatatg atgtcaactg gttttgtggg
     3841 tcggtgcaaa aa
//
LOCUS       DAWFAD010000166         3851 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWFAD010000166 DAWFAD010000000
VERSION     DAWFAD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.166"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(83..277)
                     /locus_tag="WGO49_001829"
     CDS             complement(83..277)
                     /locus_tag="WGO49_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ0306896.1"
                     /translation="MKHKLSSWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(267..2348)
                     /locus_tag="WGO49_001830"
     CDS             complement(267..2348)
                     /locus_tag="WGO49_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ0306897.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DSKQPEAQSEA"
     gene            2824..3204
                     /locus_tag="WGO49_001831"
     CDS             2824..3204
                     /locus_tag="WGO49_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ0306898.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGRRGYMPPVQNQAGPNDF
                     RAFSCENGLSVRVRRLDGGKIALRLDGRRAVLSSDVAASGERYTAEHGLFGNGTEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3337..3830)
                     /locus_tag="WGO49_001832"
                     /pseudo
     CDS             complement(3337..3830)
                     /locus_tag="WGO49_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdiI family contact-dependent growth inhibition
                     immunity protein"
BASE COUNT          904 a          958 c         1091 g          898 t
ORIGIN      
        1 cttgtcctga tttttgttaa tccactatat tataaaaatt ccgtctgaag cggcgttcag
       61 acggaatttg tttcaagcag gcttaacagc agcgtccgcc gtttgcgccg cagcggcgtt
      121 tgcggcaata gtcttgaaac tgcagcttgg tcatcacagg ggcgttggga ttatgtttgc
      181 gctgctgtgc aacgtagttt tcataatcgg gcacgcctgc catcaagttt gctgtcagct
      241 tgatggtttt ccaccaagac gagagcttat gcttcacttt gtgcctccgg ctgtttgctg
      301 tcgcggtaca ccgccgggat ttctttggcg gtcggccagc cgactttgcg tgctttgagg
      361 gcggtacgca aaccgtacgc ggcgacaatc acgacaaccg acaagaagag gatggtcaga
      421 ccggcattaa tcttgtcgtt gaagatgatt tgcgccattt cgccgatgtc tttggcaggc
      481 gcaaggactt cgtttttagc caatgcgtcg ctgtatttgc cggcgtgggc aaggaagctg
      541 atgcgcgggt cgctgtggaa cagtttttgc aggccggcgt agcaggttac gaacagtacg
      601 ccgacggcgg gaacgagtac cacccaaaca taacggtcgc gtttcatctt aatcagcacc
      661 acggcgcaca taatcaaggc cacgcctgcc agcatttggt tggcgatgcc gaacaaaggc
      721 cagagcgagt tgatgccgcc cagcgggtcg gttacgccgg tgtagaggaa gtagccccac
      781 aatgccacgg cgaagaaggt cgcaatcagg ttggcgggga tggagtcggt gttgccgaaa
      841 ggtttgtaga agatgctgcc caagtcttga atcataaaac gtgcgacgcg cgtaccggca
      901 tcgacggcgg tcaggatgaa caaggcttca aacaataggg cgaagtgata ccagaacgcc
      961 atcatcgcct cgcccggaat caggcggctc ataatgtgcg ccataccgac tgcgagggtt
     1021 ggcgcaccgc ctgcacggga aaggatggtg ttttcgccca cttccttagc ggtatgcaac
     1081 agggttgccg catcgacagg gaattgcagc ttggtggtaa tcacttcggc ggcggtgttg
     1141 gcatccgtac cgatcagggc ggcagggctg ttcatggcga agtacacgcc gggatcaagc
     1201 gatgcggcgg cggcaagcgc cataatggct acgaaacttt ccatcaacat accgccgtaa
     1261 ccaatcatac ggacgtgggt ttcgttttcc agcattttcg gcgtggtgcc ggaggaaatc
     1321 agcgcgtgga agcccgaaac cgcaccgcag gcgatggtga tgaagaggaa cgggaacaat
     1381 gcgcctgaga ataccggacc cgaaccgtcg ataaagtggg ttacggcagg catttgcaaa
     1441 gtgggattga cgatgacgat gcccaaagcc aaggccgcaa tcgtaccgat tttcaggaag
     1501 gtggagagat agtcgcgcgg agtgagcagc aaccatacgg gcaatacgga ggcgacaaag
     1561 ccgtaaatca taatcgccca agtgagctgg atgccgtcaa ggtcgaacca atgtccgatg
     1621 gaacttttcg ccacatcttc gccgtaaatt accgccagca tcagcaaaat aaagccgacg
     1681 atggaaatct cgccgatttt gcccggacgg atatagcgcg tgtaaatacc cataaacagg
     1741 gcaatcggca tagtggcggc gatggtaaac gtaccccaag ggctgtgaac aagtgctttt
     1801 acgacaatca acgccaacac cgccataatg atgaccataa tcatcaaaat accgatggag
     1861 gcaatcacgc cggggacagt gccgagttcc tgtttcacaa tatcgcccaa agacttaccg
     1921 tcacggcgca tagaaacaaa cagtaccatc atatcctgta ccgcgccggc aaaaaccacg
     1981 ccgaagataa tccacaaagt accgggcagg taacccattt gcgcagccag tacagggccg
     2041 accaaagggc ccgcgccggc aatcgcggca aagtggtgtc cgaacaatac gcccttgtgc
     2101 gtcggaacgt agtccaagcc gtcgttgtgg cgttctgccg gagtcaggcg gttaggatcg
     2161 agccgcatta cgcggttggc gatgtagagg ctgtaaaaac ggtaggcgat gcagtaaacg
     2221 gatatggcgg cggtaaccat ccataccgcg ctgacctgtt cgcctcggct gagagccaga
     2281 gtggtaaagg atgctaagcc gaccagtacc actatgcccc aaatgaggaa ggttttgagt
     2341 gatttcatcg aataaatcct tatctcacac tgtcggaata tgcctgaacg gcgggaaacg
     2401 gatgaccgcc tgccggcacg ggcacaaacc ggagtctgaa tgattgacga agtatgaaac
     2461 agtgcgcttg tcgggatttg tgccttatgc ccgcatcaaa cagtgcaggg atgcgggcgc
     2521 acaaaacgtt aaacgccgaa taggatttta acgcaaatta gcacactgat agcggttttt
     2581 acttggaatt tggaaaaaat ttacattcct tcgggcggac aggcgggttt ggatggcatc
     2641 gtcaggcaaa aggcggcgcc ggaagacggg taaagaaggg gcgcgcaatc cggattattg
     2701 attcatcgcg ggtaaattcc ggttatcggg cttgtgtgtt tgcgcgtccg tttatagtat
     2761 ggcgttgccg cagcttggaa tcagggcggt tgtttcatat cttattttta ttggggagct
     2821 tttatgaata tcaggttttt cgcgctgacc gtaccggttt tgtctttggc ggcctgtgcc
     2881 gtgccggagg cgtatgatga cggcagacgc gggtatatgc cgcctgttca aaaccaagcc
     2941 ggcccgaacg attttcgggc gttttcatgc gagaacggtt tgtctgtgcg cgtccgccgt
     3001 ttggacggtg gcaaaatcgc gttgcggctg gacggcaggc gtgccgtcct ctcttccgac
     3061 gttgccgcat ccggcgaacg ctataccgcc gaacacggtt tgtttggaaa cggaaccgag
     3121 tggcaccaga aaggcggcga agcctttttc ggctttaccg atgcctacgg caattcggtc
     3181 gaaacttcct gccgcgcccg ttaaacgggg cagcctgttt gaaccgccgt ccggattttt
     3241 cgggcggttt tttgtgtcgg atttgttttg gctgtcggct ggtttcggcg gttttcgggc
     3301 gtggcggatt atttttcgtt tcgttccggg tttatcctat cggcagcggc tcaatgccaa
     3361 ctttaaacct gctccgattt cttcagggct gttatccaat gataaaatga catcgtctgc
     3421 atcaatggca tcccacgctt ccagcttgac atggcggctc gggctgattt tcaggcagcc
     3481 gttgtgcagc caaatatccc cgctcatcat gtttttaaat agggcgcgtc tggttttata
     3541 gcccaagttc ccgcatagtt tggcaaccca atcctcatag cgttgccgaa atttttcggt
     3601 atcaaaaaaa tcttggtctt ccgaactgtc ataaacgaaa gtcctgctgt ttgccaacgc
     3661 ttgcaagacc gtcgtgccta aagtttcatt gtcggtatcc aatggcagga tatgggggga
     3721 tataggtggt ctattgccgt taaccccaaa cctgaacatg tttgaacaat cagagcttct
     3781 tcattagcat aaaaaactgc ccaataattt tgatcctgtt taaaaattat tatttgatct
     3841 ccgtaatttt g
//
LOCUS       DAWFAD010000167         3801 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWFAD010000167 DAWFAD010000000
VERSION     DAWFAD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.167"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            354..3557
                     /gene="nalP"
                     /locus_tag="WGO49_001833"
     CDS             354..3557
                     /gene="nalP"
                     /locus_tag="WGO49_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ0306899.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGTSAPDFNAGG
                     TGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLHT
                     GDFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYNE
                     NYKNYTAYMRKEAPEDGGGKDIKASFDDEAVIETEAKPTDIRHVKEIGHIDVVSHIIG
                     GRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGTT
                     SRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLFI
                     FSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSAP
                     YEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGAV
                     GVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGGS
                     QLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNGAASGGSLNSDGIVYL
                     ADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGYL
                     NRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGNA
                     ARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPGI
                     RPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDHN
                     GTGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGRSTWSE
                     NSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTLM
                     QLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLAG
                     LKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAGL
                     GADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1029 a         1030 c         1009 g          733 t
ORIGIN      
        1 atagtacgga accgattcac ttgttaaaga atcgttctct ttgagctaag gcgaggcaac
       61 gccgtactgg tttttgttca tccactataa ctagggaaat tcaaattaag ttagaattat
      121 ccctatgaga aaaagccgtc taagccggta taaacaaaat aaactcattg agctatttgt
      181 cgaaagttca aatttccatt ttaaaacaat tagtaaaatc gagtttatcc tagttgtcca
      241 agacaacccc tataataata taattcaaaa tataaaaatg ggttacatct aaacattacg
      301 gaatttttat tccctcgcct gaattctatt gtcagattca aggagacctc atcatgcgaa
      361 cgaccccaac cttccctaca aaaactttca aaccggctgc catggcgtta gctgttgcaa
      421 caacactttc tgcctgctta ggcggcggcg gcggcacttc tgcgcccgac ttcaatgcag
      481 gcggcaccgg tatcggcagc aacagcagag caacaacagc gaaatcagca gcagtatctt
      541 acgccggtat caagaacgaa atgtgcaaag acagaagcat gctctgtgcc ggtcgggatg
      601 acgttgcggt tacagacagg gatgccaaaa tcaatgcccc ccccccgaat ctgcataccg
      661 gagactttac aaacccaaat gacgcataca agaatttgat caacctcaaa cctgcaattg
      721 aagcaggcta tacaggacgc ggggtagagg taggtatcgt cgatacaggc gaatccgtcg
      781 gcagcatatc ctttcccgaa ctgtatggca gaaaagaaca cggctataac gaaaattaca
      841 aaaactatac ggcgtatatg cggaaggaag cgcctgaaga cggaggcggt aaagacatta
      901 aagcttcttt cgacgatgag gccgttatag agactgaagc aaagccgacg gatatccgcc
      961 acgtaaaaga aatcggacac atcgatgtgg tctcccatat tattggcggg cgttccgtgg
     1021 acggcagacc tgcaggcggt attgcgcccg atgcgacgct acacataatg aatacgcatg
     1081 atggaaccaa gaacgaaata atgtctgcag ccatccgcaa tgcatgggtc aagctgggcg
     1141 aacgtggcgt gcgcatcgtc aataacagtt ttggaacaac atcgagggca ggcactgccg
     1201 accatttcca aatagccaat tcggaggagc agtaccgcca agcgttgctc gcctattccg
     1261 gcggtgataa aacagacgag ggtatccgcc tgatgcaaca gagcgattac ggcaacttgt
     1321 cctaccacat ccgtaataaa aacatgcttt tcattttttc ggcaagcaat gacgcacaag
     1381 ctcagcccaa cacactgacc ctattgccat tttatgaaaa agatgctcaa aaaggcatta
     1441 tcacagtcgc aggcgtagac cgcagtggag aaaagttcaa tggctccaac cattgcggaa
     1501 ttactgccat gtggtgccta tcggcaccct atgaagcaag cgtccgtttc acccgtacaa
     1561 acccgattca aattgccgga acatcctttt ccgcacccat cgtaaccggc acggcggctc
     1621 tgctgctgca gaaatacccg tggatgagca acgacaacct gcgtaccacg ctgctgacaa
     1681 cggctcagga catcggtgca gtcggcgtgg acagcaagtt cggctgggga ctgctggatg
     1741 cgggtaaggc catgaacgga cccgcgtcct ttccgttcgg cgactttacc gccgatacga
     1801 aaggtacatc cgatattgcc tactccttcc gtaacgacat ttcaggcacg ggcggcctga
     1861 tcaaaaaagg cggcagccaa ctgcaactgc acggcaacaa cacctatacg ggcaaaacca
     1921 ttatcgaagg cggttcgctg gtgttgtacg gcaacaacaa atcggatatg cgcgtcgaaa
     1981 ccaaaggtgc gctgatttat aacggggcgg catccggcgg tagcctgaac agcgacggca
     2041 ttgtctatct ggcagatacc gaccgatccg gcgcaaacga aaccgtgcac atcaaaggcg
     2101 atctgcagct gggcggcgaa ggtacgctgt acacacgttt gggcaaactg ctgaaagtgg
     2161 acggtacggc gatgaccggc ggcaagctgt acatgtcggc acgcggcaaa ggggcaggct
     2221 atctcaaccg taccggacaa cgtgttccct tcctgagtgc cgccaaaatc gggcgggatt
     2281 attctttctt cacaaacatc gaaaccgacg gtggtctgct ggcttccctc gacagcgtcg
     2341 aaaaaacagc gggcagtgaa ggcgacacgc tgtcctatta tgtccgtcgc ggcaatgcgg
     2401 cacggactgc ttcggcagcg gcacattccg cgcccgccgg tctgaaacac gccgtagaac
     2461 agggcggcag caatctggaa aacctgatgg tcgaactgga tgcctccgaa tcatccgcaa
     2521 cacccgagac ggttgaaact gcggccgccg accgcacaga tatgccgggc atccgcccct
     2581 acggcgcaac tttccgcgca gcggcagccg tacagcatgc gaatgccgcc gacggtgtac
     2641 gcatcttcaa cagtctcgcc gctaccgtct atgccgacag taccgccgcc catgccgata
     2701 tgcagggacg ccgcctgaaa gccgtatcgg acgggttgga ccacaacggc acgggtctgc
     2761 gcgtcatcgc gcaaacccaa caggacggtg gaacgtggga acagggcggt gttgaaggca
     2821 aaatgcgcgg cagtacccaa accgtcggca ttgccgcgaa aaccggcgaa aatacgacag
     2881 cagccgccac actgggcatg ggacgcagca catggagcga aaacagtgca aatgcaaaaa
     2941 ccgacagcat tagtctgttt gcaggcatac ggcacgatgc gggcgatatc ggctatctca
     3001 aaggcctgtt ctcctacgga cgctacaaaa acagcatcag ccgcagcacc ggtgcggacg
     3061 aacatgcgga aggcagcgtc aacggcacgc tgatgcagct gggcgcactg ggcggtgtca
     3121 acgttccgtt tgccgcaacg ggagatttga cggtcgaagg cggtctgcgc tacgacctgc
     3181 tcaaacagga tgcattcgcc gaaaaaggca gtgctttggg ctggagcggc aacagcctca
     3241 ctgaaggcac actggtcgga ctcgcgggtc tgaagctgtc gcaacccttg agcgataaag
     3301 ccgtcctgtt tgcaacggcg ggcgtggaac gcgacctgaa cggacgcgac tacacggtaa
     3361 cgggcggctt taccggcgcg actgcagcaa ccggcaagac gggggcacgc aatatgccgc
     3421 acacccgcct ggttgccggt ctgggcgcgg atgtcgaatt cggcaacggc tggaacggct
     3481 tggcacgtta cagctacgcc ggttccaaac agtacggcaa ccacagcgga cgagtcggcg
     3541 taggctaccg gttctgacgg acaggaagca gacagccgca aagatcacgg tctttgcggc
     3601 tgtttcttat gaaaagaaaa ccctattcca attgcctgct tctattgttt caagacttct
     3661 tccaaagatt cggcattaat cagatgtata gcggattaac aaaaatcagg acaaggcggc
     3721 gaagccgcag acagtacaaa tagtacggaa ccgattcact cggtgcttga gcaccttaga
     3781 gaatcgtttc tttgagctaa g
//
LOCUS       DAWFAD010000168         3778 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWFAD010000168 DAWFAD010000000
VERSION     DAWFAD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.168"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..184)
                     /locus_tag="WGO49_001834"
     CDS             complement(<1..184)
                     /locus_tag="WGO49_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HEZ0306900.1"
                     /translation="MKPDTLQSYGSTTRFLHWTIAACYLFMFATVIAWNIDENLKFLI
                     NSHKAVGILLLILSVWR"
     gene            454..864
                     /locus_tag="WGO49_001835"
     CDS             454..864
                     /locus_tag="WGO49_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0306901.1"
                     /translation="MNHIARQFAILLGEGLKPLGIAPAQFPILLELWQKDGLSQQELV
                     ERADLKQATIANTLARMERDGLITREPNPDDARSRLIMLTEQARALQQQSTEIAQAIN
                     QTALSDLSANEQKLFLEMAAKIVARQQEMITGEK"
     gene            complement(979..1530)
                     /locus_tag="WGO49_001836"
                     /pseudo
     CDS             complement(979..1530)
                     /locus_tag="WGO49_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520560.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(1571..2230)
                     /gene="ung"
                     /locus_tag="WGO49_001837"
     CDS             complement(1571..2230)
                     /gene="ung"
                     /locus_tag="WGO49_001837"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ0306902.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKGRLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            2311..2718
                     /locus_tag="WGO49_001838"
     CDS             2311..2718
                     /locus_tag="WGO49_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ0306903.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYVEILRHTGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIIANP"
     gene            2733..3680
                     /locus_tag="WGO49_001839"
     CDS             2733..3680
                     /locus_tag="WGO49_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ0306904.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
BASE COUNT          928 a          936 c          971 g          943 t
ORIGIN      
        1 cgcgccaaac agacaatatc agcagcagga ttccgactgc tttgtgcgaa ttgattagaa
       61 atttcaggtt ttcgtcgata ttccacgcaa tgacggtagc gaacataaac aggtagcaag
      121 ctgctattgt ccaatgcagg aagcgggttg ttgagccgta gctttgcaaa gtgtcgggtt
      181 tcatgatgct ttctcttgat aaagggttag tcggatttcg gtatggctta cagataggaa
      241 aaccttcttt aaagtattga agttcataag tgttcttgaa catttaagat aggtcgtctg
      301 aaagtgttta gatagcaagc tattaagctg caataagtat agcgtgctat gtattaagat
      361 agcaagctat atttgtgata taatccgcaa caatttgatt tgaaagaaaa caaagtggac
      421 gaagttttca aaactgaatc ggcaggctat ttgatgaacc acattgcccg ccaatttgcc
      481 attttattgg gcgaaggttt aaagcctttg ggcatcgccc ccgcgcaatt tccgatattg
      541 ctggaactgt ggcaaaaaga cggtttgagc cagcaggaat tggtggaacg cgccgatttg
      601 aagcaggcaa cgattgccaa cactttggcg cggatggagc gcgacggact gattacgcgc
      661 gaaccgaatc ccgacgatgc gcgcagccgc ctgattatgc tgacggagca agctcgcgcc
      721 ttgcagcagc agtccacgga aattgcccaa gccatcaacc aaacggcttt gtcggatttg
      781 tcggcaaacg aacaaaagtt gtttttggaa atggcggcaa aaattgtggc gcgacagcaa
      841 gagatgatta cgggcgagaa gtgagggggc cgatggcgca acttggaatt ttaaagtgca
      901 ggctgctttt gaggtcgtct gaaagcagcc tgcgctttga aaacaaggtg caggctgctt
      961 agggtaatgt gtcgtttatc aatccagttt caggatttct ccgtcttccg gaacgttcac
     1021 acggctttca atgccttgac cgcgtataaa tttacgcata tcggtgcggc tgacggcggt
     1081 atgattcacg gtatccatgt gtacggtaat gcctgaaatc agcgcgtagc ccgtgttcat
     1141 aatcagataa tcgggtttgt agcggttcaa tgctttgttt acatctgccg tccacacagt
     1201 atcacccata atataagcgg ttttgtgtcc gctgctttgg aatactacac ccattgcatc
     1261 acctaagatt tcggctagct gcgggttggc atacatggct tctgtaccat gtacgccgcc
     1321 ggttttgctg atggttacgc cgttgaagac agtgctgccg ttcaacacgc gtacatcagt
     1381 aaatccttgg ctgcggattt ttgccgcgtc ggcttggtgc tgcacatata cgggcaggtt
     1441 tttcggaatg gaacgtgcgg cggtttcgcc ccaatggtct tcgtgggtat gggtaacgat
     1501 aactgcatcc acaccgtaga ttgactggtt ccataagtgc agtttaagca acttggcatt
     1561 cagacggcat tcacagcttc cagtttatcg gatcgatacc gtgccggctc aaatagctgt
     1621 ttgcctgtga aaaatggcgg cagccgaaaa aaccgcgata tgccgacaga ggagacggat
     1681 gcggtgcggt caatatcaaa tgattttgac tgtctatcag cctccctttt tgttgtgcat
     1741 acccacccca caacatgaag acaaggtgct tgcggtgtgt cgcaagctgc ctgatgacgg
     1801 tatcggtaaa gcgttcccag cctaaaaggg cgtgcgaatg cgcctgtcct gcacgcaccg
     1861 tcaaaaccgt gttcagaagc aatacgccct gctccgccca cgctgtcaga cagccgtgcg
     1921 cgggaatgga aaagccttcg atgtcggttt ccaactcctt gtagatattg agtaaagacg
     1981 gcggtatgcg gataccctgc cggacggaaa atgccaaacc gtgcgcctgc cctgccccgt
     2041 gatacggatc ttgtccgaga atgacggctt tgacccggtc gaacgctgtc aggcggaatg
     2101 cgttgaacac atccgccgcc ggcggataga tgatttgtcc cgacaaacgt tcctgcctga
     2161 ctgcatttaa aatttcctga aaatacggct gctgtttttc gccgccgagt gcatcgtgcc
     2221 aagtgtccat atctgcccta tcctttcaaa ttcggctata ctgctgatat tgcgttattt
     2281 tgtcaaacga cgcgctttgt ggggaaatgt atgactgtat ggtttgttgc cgctgttgcc
     2341 gtcttaatca tcgaattatt gacgggaacg gtttatcttt tggttgtcag cgcggctttg
     2401 gcgggttcgg gcattgctta cgggctgacc ggcagtacgc ctgccgccgt cttgaccgcc
     2461 gctctgcttt ccgcgctggg tatttggttc gtacacgcca aaaccgccgt gggaaaagtt
     2521 gaaacggatt catatcagga tttggatgcc ggacaatatg tcgaaatcct ccgacacaca
     2581 ggcggcaacc gttacgaagt tttttatcgc ggtacgcact ggcaggctca aaatacgggg
     2641 caagaagagc ttgaaccagg aactcgcgcc ctaatcgtcc gcaaggaagg caaccttctt
     2701 atcatcgcaa acccttaaca ctcggaggaa ttatggaatt tttcattatc ttgttggtag
     2761 ccgttgccgt tttcggcttc aaatcctttg tcgtcatccc acagcaggaa gtccacgttg
     2821 tcgaaaggct tggacgtttc catcgcgcct tgacggccgg tttgaatatt ttgattccct
     2881 ttatcgaccg cgtcgcctac cgtcattcgc tgaaagaaat ccctttagac gtacccagcc
     2941 aggtctgcat cacgcgcgac aacacccagc tgacggttga cggcatcatc tatttccaag
     3001 taaccgatcc caaactcgcc tcatacggtt cgagcaacta cattatggcg attacccagc
     3061 ttgcccaaac gacgctgcgt tccgttatcg ggcgtatgga gttggacaaa acgtttgaag
     3121 aacgcgacga aatcaacagc accgtcgtct ccgccctcga tgaagccgcc ggagcttggg
     3181 gcgtgaaggt tttgcgttat gagattaaag acttggttcc gccgcaagaa atccttcgct
     3241 caatgcaggc gcaaattact gccgaacgcg aaaaacgcgc ccgtatcgcc gaatccgaag
     3301 gtcgtaaaat cgaacaaatc aaccttgcca gtggtcagcg cgaagccgaa atccaacaat
     3361 ccgaaggcga ggctcaggct gcggtcaatg cgtcaaatgc cgaaaaaatc gcccgcatca
     3421 accgcgccaa aggtgaagcg gaatccttgc gccttgttgc cgaagccaat gccgaagcca
     3481 tccgtcaaat tgccgccgcc cttcaaaccc aaggcggcgc ggatgcggtc aatctgaaga
     3541 ttgcggaaca atacgtcgct gcgttcaaca atcttgccaa agaaagcaat acgctgatta
     3601 tgcccgccaa tgttgccgac atcggcagcc tgatttctgc cggtatgaaa attatcgaca
     3661 gcagcaaaac cgccaaataa gccgtgatga aaatgccgtc tgaagctctg ttttcggggt
     3721 tcagacggca tttttattta aacccgggca aggttgtatt ccgcttcaaa ttatagtg
//
LOCUS       DAWFAD010000169         3555 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWFAD010000169 DAWFAD010000000
VERSION     DAWFAD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.169"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            109..1224
                     /gene="lst"
                     /locus_tag="WGO49_001840"
     CDS             109..1224
                     /gene="lst"
                     /locus_tag="WGO49_001840"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ0306905.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKTGDETGGTVRILLGSPDKEMKEISEKAAKDFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKN"
     gene            complement(1389..1847)
                     /locus_tag="WGO49_001841"
     CDS             complement(1389..1847)
                     /locus_tag="WGO49_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0306906.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFADSSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            1966..2787
                     /locus_tag="WGO49_001842"
     CDS             1966..2787
                     /locus_tag="WGO49_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0306907.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPNMVARGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDDEKAAGVYEGVEFIRPEDIAETALW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            complement(2838..3284)
                     /gene="yciA"
                     /locus_tag="WGO49_001843"
     CDS             complement(2838..3284)
                     /gene="yciA"
                     /locus_tag="WGO49_001843"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ0306908.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIAQGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKEGNPILAGLLPTP"
BASE COUNT          840 a          772 c         1022 g          921 t
ORIGIN      
        1 ccactataat attcggtaac tgtcggaata tctgctaaaa ttccgcattt ttccgtcccg
       61 ggacactcgg ggcgtatgtt caatttgtcg gaatggagtt ttagggatat gggcttgaaa
      121 aaggcttgtt tgaccgtgtt gtgtttgatt gttttttgtt tcgggatatt ttatacattt
      181 gaccgggtaa atcaggggga aaggaatgcg gtttccctgc tgaaggacaa actcttcaat
      241 gaagaggggg aaccggtcaa tctgattttc tgctatacca tattgcagat gaaggtggcg
      301 gaaaggatta tggcgcagca tccgggggag cggttttatg tggtgctgat gtctgaaaac
      361 aggaatgaaa aatacgatta ttatttcaat cagataaagg ataaggcgga gcgggcgtat
      421 tttttccacc tgccctacgg tttgaacaaa tcgtttaatt tcattccgac gatggcggag
      481 ctgaaggtaa agtcgatgct gctgccgaaa gtcaagcgga tttatttggc aagtttggaa
      541 aaagtcagca ttgccgcctt tttgagcact tacccggatg cggaaatcaa aacctttgac
      601 gacgggacag gcaatttaat tcaaagcagc agctatttgg gcgatgagtt ttctgtaaac
      661 gggacgatca agcggaattt tgcccggatg atgatcggag attggagcat cgccaaaacc
      721 cgcaatgctt ccgacgagca ttacacgata ttcaagggtt tgaaaaacat tatggacgac
      781 ggccgccgca agatgactta cctgccgctg ttcgatgcgt ccgaactgaa gacgggggac
      841 gaaacgggcg gcacggtgcg gatacttttg ggttcacccg acaaagagat gaaggaaatt
      901 tcggaaaagg cggcaaaaga tttcaacata caatatgtcg cgccgcaccc ccgccaaacc
      961 tacgggcttt ccggcgtaac cacattaaat tcgccctatg tcatcgaaga ctatattttg
     1021 cgcgaaatta agaaaaaccc gcatacgagg tatgaaattt ataccttttt cagcggcgcg
     1081 gcgttgacga tgaaggattt tcccaatgtg cacgtttacg cattgaaacc ggcttccctt
     1141 ccagaagatt attggctcaa gccggtgtat gccctgttta cccaatccgg catcccgatt
     1201 ttgacatttg acgataaaaa ttaatcgcat agcaaatcaa aatagaaaat ggcggagtgc
     1261 gtggggtaaa aataaggata gcgttttttc atttggattg acgataattt ctgattgctt
     1321 tgcgtgtgct gaaatggcaa agaaaatgcc gtctgaagtc ttcagacggc attgttttgt
     1381 tttggatgtt attcgggcgc gcggaagctg tcgtggcagg atttgcagct tgcgccggtt
     1441 tcgccgtagg cggctttgat ttcgtccagt ttgccggttt gggcggcggc gttgagtttt
     1501 tcgacggcgg cggcgaattt tgttttttcg gcttcaaatt ttgcaccatc cgaccaaacg
     1561 gcgggcagtg cgcgcccgtt gccttgcggg tcggactcaa aaagcgtaaa cggttttttg
     1621 ctgctgtcgg caaacgccgc tgccgcctgt ttgaattttt cgacatcgta aggttcttcg
     1681 cctttgacca ttttgcccat acgcgagaag tcgggcatca tagatttgaa cgcggcggtg
     1741 cggttttcgg aaatttcgcc tttgggttgg gaaggtattc cgctgcctcc gcaggcggaa
     1801 aggagcagtg tgatggcggc agcagcaagg ctgatttggg ttttcatatt gaatgtgtcc
     1861 tgtcgtggtg gtatggttgt tgtcattttc agccggcgcg atgccgtctg aagggtatta
     1921 tgatacctga aacaggtttg ggaaacgaga aagaaaggaa aaacaatggc tgttttaatt
     1981 accggtgctt cggcaggatt cggcgaagcg atgtgccgcg cgtttgtcgg ggcgggatac
     2041 cgcgttatcg gtgcggcgcg tcgtgcggac aggcttcagg ccttggcgga tgaattgggt
     2101 gctttgtttt accctttgga aatggacgtg tcgcgccgcg agtcggtgga aaacgcctta
     2161 aacggcatcc ctgatgaatt ttccgacatc gactgcctca tcaacaatgc cgggctggct
     2221 ttgggtctgg acacggcgga caaggcggat tttgaagatt gggaaacaat gattcaaacc
     2281 aatgttttgg gtttgacgtt tctaacgcgc aagattttgc cgaatatggt ggctcggggc
     2341 ggcggttatg tgatgaattt gggttcgatt gcaggcaatt atgcttatgc cggcagcaac
     2401 gtttacgggg cgaccaaggc gtttgtgcgc cagttcagcc tgaatttgcg cgcggagttg
     2461 gcggataaga acatccgcgt taccaatatc gagccgggtt tgtgcggcaa tacggagttt
     2521 tccaacgtgc gcttcaaagg cgatgacgag aaggcagcag gcgtgtatga gggtgtggaa
     2581 tttatccgcc ccgaagatat tgcggaaacc gcattgtggc tgtaccgccg gccggcgcat
     2641 atgaatgtga acacgattga aattatgccc gtggcgcaga cttttgcagg aatgaaggtg
     2701 ataaaaaaag ccgtgccgga agtgcgggaa gactttgaaa aacagagtat gtcgctgttt
     2761 tcccgcatca gatcttggtt caaatgatgc ggaatgccgt ctgaagacag tttcagacgg
     2821 catttttacg ggcattttta cggagtaggc aataagcccg ccaatatggg gttgccttct
     2881 ttcggaatcg ggcgcggatt gccttccgca tcgatggcga cataagtgaa cacggcttcg
     2941 gttacgaggt ggcggtcttc ggtaacgcaa tcgttcatca aagttttcac ccagacttcg
     3001 actttaagct ggagggaagt attgcccacg cggacgcaat gcccgtagca gcagacgacg
     3061 ttgccgacct tgaccgggcg gatgaagttc atttcctgaa cggcgacggt aacgatgcgt
     3121 ccttgtgcga tttccgctgc caatatgccg ccgcccaaat ccatctgcga cataatccag
     3181 ccgccgaaaa tgtcttggtt gggattggta tcgcgcggca tagcgacggt acgcaggagc
     3241 agttcgcctt gagggcgttg gcggttgcct tcttcgtgct gcataaagtt tccttgtttt
     3301 attgaaagat aaatcgaacc tgcacccctg cccgaaacgg gaagtcggga agtgcgtagt
     3361 ttaacccatt tgagacatac aagggcgggc gtgggacaga ttcgcaaggc gtattgtagg
     3421 gcggggctgt agagtgggct tcagtccgcc aatcccgcca aatcctaccc taagcaactg
     3481 aaccgtcatt cccacgaaag tgggaatcta gaatctcggg ggttcagtca tttccgatag
     3541 attcccgccg cgtca
//
LOCUS       DAWFAD010000170         3524 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWFAD010000170 DAWFAD010000000
VERSION     DAWFAD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.170"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(214..1278)
                     /locus_tag="WGO49_001844"
     CDS             complement(214..1278)
                     /locus_tag="WGO49_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0306909.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRENRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIIH
                     NGIDTDRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGH
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTENIASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAAH
                     QCVAARFTINHTADKLLDAI"
     gene            complement(1279..2037)
                     /locus_tag="WGO49_001845"
     CDS             complement(1279..2037)
                     /locus_tag="WGO49_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0306910.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFQRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YATYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            complement(2112..2187)
                     /locus_tag="WGO49_001846"
     tRNA            complement(2112..2187)
                     /locus_tag="WGO49_001846"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2152..2154),aa:Ile,seq:cat)
     gene            complement(2241..3377)
                     /locus_tag="WGO49_001847"
                     /pseudo
     CDS             complement(2241..3377)
                     /locus_tag="WGO49_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            complement(3322..>3524)
                     /locus_tag="WGO49_001848"
     CDS             complement(3322..>3524)
                     /locus_tag="WGO49_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0306911.1"
                     /translation="SLSSCDKLDVSGFIHYRINHSKEFADRQNHINGIENFWNQAKRV
                     LRKYSGLARTFPRIGSATGCAD"
BASE COUNT          800 a          854 c          932 g          938 t
ORIGIN      
        1 tctactatac attgtaacaa agcacttgat gcggcagcac aagccgggga agacgctgca
       61 aaactttgcc aataggctgc gtaatatcgg gggatattgg gattttctta tttttaagga
      121 tggattcggg gtgtttcttt ccaatgaacg aatataattt ggcggtttgt tgcatcaaag
      181 gctttcagac ggcatatgcc gtctgaaagc agtttatatt gcatccaata atttgtcggc
      241 ggtatggttg atggtaaaac gggcggcgac gcattgatga gctgccgttg ccagtgcgtt
      301 ttttgcttcg gggtttaaga cgaggcgttc gatttcgtcc gccaaagatt caggcgtaag
      361 cctatccagc agaatccccg attgatgatg ttcgacaatt tccttttgcg cccccaaagt
      421 attggaaatc accgccgttc ggcagtacat cgcctcgcat aatgacaaac cgaatgcctc
      481 cgggacgagg ctgggcaaaa ccacgacatc gctttggcgg taaaacgaag caatgttttc
      541 ggtaaaccct tcaaaagaaa caaatggttc tgctcctgaa gcagatacgt cccgcttcag
      601 gcggcacata taatcaggat gtccgtgccc tgccaatttg agtctgattt gaggatattt
      661 ccgatgcaat atcacacagg cttcaattag gttttccaat cctttttccg gactgatcct
      721 gccggcgtag gcgacggtaa aaaaacggct gtcgggtttt tcttgagagg gagggaaccg
      781 atcggtatcg ataccgttat gaataatccg gtatttttct ttaaagggat tgtcggcggt
      841 ttgcacatcg taaaccagac gggaaacgca aataaagcgg tctgtgtttt tctgtatcag
      901 gcggtggtaa aaatcggttt tgttggcgac gacattatgt ttgacaaata tcaggcgcac
      961 cccggtcagt tttttcaaaa gtatggataa ggcggcaatt ttgccggtgt gtatcatcag
     1021 gtgggaaatg cggttttccc ggataaagcg ggtaagcgcg cagagcgaaa acagcccgtt
     1081 gagcgtgtga aggcgtgtcg tgaaaacgga agaaacttcg gaaaagcgtc tgtgcatcaa
     1141 ctcattattt ttattgacgg cggtaaacat tgtgcagccc cgaagcccca atgcttttga
     1201 aacatcatag acatattgtt cgcccccacc ccaaatcttg gccgatacga tatttaatat
     1261 cctgaattct ttttccattt aaaattttcc gccagatttg tacagataat atagtttgac
     1321 atatttaatc atcgtgtaat agctgtggtt gacggacata atccaaccca tttttccatc
     1381 aagaaaccct ttgttcagaa tataaatttt gaaaaaaccc caaatcggac ggaggataat
     1441 gtccctaacg aaacgcacgg gctttccctg ctctcggtat ttctcggctg agatggaagt
     1501 atatttgttg aacttgttga aatattgttc ccagttgtcg tacgtataat ggtacataaa
     1561 atgtttcaga cggcgtttgg ggtagggggt ttgtacggtt tcgtgtactt tgccttgcac
     1621 cgaactgtct ttcttcggca tcaaacggca tacgctgtcg ggacgcatcg cgccgtgtgt
     1681 ggcgggatgg ttggggaaaa ggttgcggcg ctcgacaaaa taggtggcat aatcgccggt
     1741 ttggacaatt tttgagattt catcagatag ttccggcgtg cagcgttcgt ctgcatcaat
     1801 caggaaaacc cattctccgc ctgcctgttc gatggcaaat gttttttgcg ctccgaaatc
     1861 cccgttcaaa tgccgttgga agacttttgc acccaaaccc tcggcaattt cggcagtatt
     1921 gtcggtgctg tggtcgtcga taacgataac ttctttatcg aaacggcaac tttcaataca
     1981 ttcccgaatg tggtttgctt cgtttttggc aacaatcaat acgctgactt ttttcattat
     2041 caaggtttct caaagcattt tggtttcaca gggcggcaaa aaagcccaaa catacgtccg
     2101 ggcttgggaa ttggtgggtc cggtggggtt cgaacccacg accaagggat tatgagtccc
     2161 ctgctctaac ccctgagcta cagacccgtc aagagtaggc gcgtattgta tagcatagcg
     2221 gtgggattga caaacttatt tcatccgatg attaagacgc agttgcttcc cccgaaggca
     2281 aacgatgagc tggcggcaat gcgtttttcg gtttcccagc ggctgccgct gccggtcagg
     2341 ttgatggcgg gcaggttggg gtcgttctgc ccgtcccaaa gccggggcgg aagttttcct
     2401 tcgggattgc tttgccggtt ggcaatgccc cacgcgaacg cggcttcgat tgcgtccgcc
     2461 gcgcccagcg tgtgtccggt ttgcggcttg gtggacgtgc agggcgtatt gttgccgaaa
     2521 accgctgcaa cggcgcggct ttccatattg tcgttgtggt gcgtcccggt gccgtgcaga
     2581 ttaatccagc cgatgtcttc gggcgcaaga cctgcgtgtt gcaatgccgt ctgaaaggca
     2641 aggattgcgc cttacgcgtc ggggcggggc gtggaaatat ggtaggcatc gctgttggct
     2701 ccgtagccga cgagttcggc gtaaattttc gcaccgcgcc gtttggcgtg ttccaattct
     2761 tccagcacga aaatccccgc gccttcgccg atgaccagcc cgtcgcggtt cgcgtcgtat
     2821 gggcgcgggg ttttttccgg ttcgccgttg cggcggctgg cggcataaag cgagtcgaaa
     2881 acatacactt cggacgggaa aaattcttcg cctccgcccg ccagcatcat atcggtcaga
     2941 ccgtatttga tggcttcgta ggcataacct atgccttggc tgccggacga acacgcgctc
     3001 gatgtcggga tgatgcgtcc tttcagcccg aaaaagatgc cgatattggc ggcggcggtg
     3061 tgcggcatca tacgcacata ggtgttggca ctgaagttgc gcgacatgcc ggtcagcaac
     3121 aattcgccta catcgccgat gtctttggtg ctgccgctgg aagagccgca ggcaacgccc
     3181 atgcgtccgt cagtaatgct ttcgtctccg agcaaaccgg catccgtcaa agcctgctcc
     3241 gccgcatcga cgcacaaata cgacacacgc cccatacttc tgagctgctt gcgcgtccaa
     3301 tgtttcggcg gcgcgtaatt ttcaatcggc gcacccagtt gcgcttccaa ttcggggaaa
     3361 cgttcgtgcc aatccactat attttcgcaa gacgcgtttt gcctgattcc aaaagttctc
     3421 aatgccgtta atgtggttct gacggtctgc aaattccttg gaatggttga tgcggtaatg
     3481 gataaaaccg ctcacgtcca acttgtcgca gctgctcaga ctat
//
LOCUS       DAWFAD010000171         3427 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWFAD010000171 DAWFAD010000000
VERSION     DAWFAD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.171"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            314..3079
                     /locus_tag="WGO49_001849"
     CDS             314..3079
                     /locus_tag="WGO49_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0306912.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQQYFVQTGGLKFNSNSGKWER
                     DLQRQFWKTSYYKQYKDPQKLKKYIEDQDKDWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYKKHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNFRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNADGLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
     gene            3122..3364
                     /locus_tag="WGO49_001850"
     CDS             3122..3364
                     /locus_tag="WGO49_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306913.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPQKRESRHSMLRQFIEND"
BASE COUNT          974 a          846 c          828 g          779 t
ORIGIN      
        1 gcctgcgcgg gaatgacggc tgcagatgcc cgacggtctt tatagcggat taataaaaat
       61 caggacaagg cggcgagcca cagacagtac aaacagtacg gaaccgattc acttggtgct
      121 tcagcacctt agagaatcgt tctctttgag ctaaagcgag acaacgctgt actggttttt
      181 gttaatccac tataaatatc caattgaaat cttcagacgg tatatcaaat ttacactttt
      241 ttaatgttta tgccgcctga aaaaaatgct agtatatttc ctaattgtct gactgtttat
      301 tgttgaggaa aatatgagat cttctttccg gttgaagccg atttgttttt accttatggg
      361 tgttatgcta tatcatcata gttatgccga agatgcaggg cgcgcgggca gcgaggcgca
      421 gatacaggtt ttggaagatg tgcacgtcaa ggcgaagcgc gtaccgaaag acaaaaaagt
      481 gtttaccgat gcgcgtgccg tatcgacccg tcaggatata ttcaaatcca gcgaaaacct
      541 cgacaacatc gtacgcagca tccccggtgc gtttacacag caagataaaa gctcgggcat
      601 tgtgtctttg aatattcgcg gcgacagcgg gttcgggcgg gtcaatacga tggtggacgg
      661 catcacgcag accttttatt cgacttctac cgatgcgggc agggcaggcg gttcatctca
      721 attcggtgca tctgtcgaca gcaattttat tgccggactg gatgtcgtca aaggcagctt
      781 cagcggctcg gcaggcatca acagccttgc cggttcggcg aatctgcgga ctttaggcgt
      841 ggatgacgtc gttcagggca ataataccta cggcctgctg ctaaaaggtc tgaccggcac
      901 caattcaacc aaaggtaatg cgatggcggc gataggtgcg cgcaaatggc tggaaagcgg
      961 agcatctgtc ggtgtgcttt acgggcacag caggcgcagc gtggcgcaaa attaccgcgt
     1021 gggcggcggc gggcagcaca tcggaaattt tggcgcggaa tatctggaac ggcgcaagca
     1081 gcaatatttt gtacaaacag gcgggttgaa attcaattcc aacagcggaa aatgggaacg
     1141 cgatttgcaa aggcaattct ggaaaaccag ttactataaa caatacaaag acccccagaa
     1201 actgaaaaaa tacatcgaag accaagataa agactggcgg gaaaacctgg cgccgcaata
     1261 cgacatcacc cccatcgatc cgtccagcct gaagcagcag tcggcaggca atctgtttaa
     1321 attggaatac gacggcgtat tcaataaata cacggcgcaa tttcgcgatt taaacaccaa
     1381 aatcggcagc cgcaaaatca tcaaccgcaa ttatcaattc aattacggtt tgtctttgaa
     1441 ctcgtatacc aacctcaatc tgaccgcagc ctacaattcg ggcaggcaga aatatccgaa
     1501 agggtcgaag tttacaggct gggggctttt aaaggatttt gaaacctaca acaacgcaaa
     1561 aatcctcgac ctcaacaaca ccgccacctt ccggctgccc cgcgaaaccg agttgcaaac
     1621 cactttgggc ttcaattatt tccacaacga atacggcaaa aaccgctttc ctgaagaatt
     1681 ggggctgttt ttcgacggtc ctgatcagga caacgggctt tattcctatt tggggcggtt
     1741 taagggcgat aaagggctgc tgccccaaaa atcaaccatt gtccaaccgg ccggcagcca
     1801 atatttcaac acgttctact tcgatgccgc gctcaaaaaa gacatttacc gcttaaacta
     1861 cagcaccaat accgtcggct accgtttcgg cggcgaatat acgggctatt acagctcgga
     1921 aggcgaattt aagcgggcat tcggagaaaa ctcgccgaca tacaagaaac attgcaaccc
     1981 gagctgcggg ctttatgaac ccgtattgaa aaaatacggc aaaaagcgcg ccaacaacca
     2041 ttcggtcagc attagtgcgg acttcggcga ttatttcatg ccgttcgcca gctattcgcg
     2101 cacacaccgt atgcccaaca tccaagaaat gtatttttcc caaatcggcg actccggcgt
     2161 tcacaccgcc ttaaaaccag agcgcgcaaa cacttggcaa tttggcttca atacctataa
     2221 aaaaggattg ttaaaacaag atgatacatt aggattaaaa ctggtcggct accgcagccg
     2281 catcgacaac tacatccaca acgtttacgg gaaatggtgg gatttgaacg gggatattcc
     2341 gagctgggtc agcagcaccg ggcttgccta caccatccaa catcgcaatt tcaaagacaa
     2401 agtgcacaaa cacggttttg agttggagct gaattacgat tatgggcgtt ttttcaccaa
     2461 cctttcttac gcctatcaaa aaagcacgca accgaccaac ttcagcgatg cgagcgaatc
     2521 gcccaacaat gcgtccaaag aagaccaact caaacaaggt tatgggttga gcagggtttc
     2581 cgccctgccg cgagattacg gacgtttgga agtcggtacg cgctggttgg gcaacaaact
     2641 gactttgggc ggcgcgatgc gctatttcgg caagagcatc cgcgcgacgg ctgaagaacg
     2701 ctatatcgac ggcaccaacg ggggaaatac cagcaatttc cggcaactgg gcaagcgttc
     2761 catcaaacaa accgaaactc ttgcccgcca gcctttgatt tttgattttt acgccgctta
     2821 cgagccgaag aaaaacctta ttttccgcgc cgaagtcaaa aatctgttcg acaggcgtta
     2881 tatcgatccg ctcgatgcgg gcaatgatgc ggcaacgcag cgttattaca gctcgttcga
     2941 cccgaaagac aaggacgaag acgtaacgtg taatgctgat ggcttgtgca acggcaaata
     3001 cggcggcaca agcaaaagcg tattgaccaa ttttgcacgc ggacgcacct ttttgatgac
     3061 gatgagctac aagttttaaa ggcagcccgc gttttgtgga aaaccgcaaa aaaaagccaa
     3121 aatgccgtct gaaagccctt cagacggcat ttgtttcccc aaacgcatca tcctgccgca
     3181 agcctatgcc aatccgtttt atcgcatcgg caactcaaag aaaaatccat ttcattccca
     3241 cgcagggaag ccggtttttg atttcggtta tttttggttg tttcgggtaa tttatgagtc
     3301 gtcattcccg caaaagcggg aatctagaca ttcaatgcta aggcaattta tcgaaaatga
     3361 ctgaaactca aaaaactgga ttcccacttt cgtgggaatg acgcggtgca ggtttccgta
     3421 tggatgg
//
LOCUS       DAWFAD010000172         3382 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWFAD010000172 DAWFAD010000000
VERSION     DAWFAD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.172"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..158)
                     /locus_tag="WGO49_001851"
     CDS             complement(<1..158)
                     /locus_tag="WGO49_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0306914.1"
                     /translation="MSTFFQQTAQAMIAKHIDCFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRP"
     gene            complement(382..2514)
                     /locus_tag="WGO49_001852"
     CDS             complement(382..2514)
                     /locus_tag="WGO49_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ0306915.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
BASE COUNT          962 a          900 c          772 g          748 t
ORIGIN      
        1 ggacggccgc ggtggtctct gaggtaacgg gttctttgac ggttcaggta ttgttcgatc
       61 ggctgccaat caattacctg gtccaacttc aatagtggga agcagtcgat gtgcttggca
      121 atcatggctt gcgcggtttg ttgaaagaag gtgctcatgg aaaatcccct aaatgtcttg
      181 gtgggaattt aggggatttt ggggaatttt gcaaaggtct ctagatgagt gaaaaagaag
      241 tgcaggctgc ctaaaaagac agaaaaagtc tttccggcag cctgcacttt ggtttcattt
      301 cagtcagtaa acccagtaaa cgacggtctg aaaacgcaga acgttacgaa aaaagcagcc
      361 tacacgccca tcccccgcct tctacccgtt ctgtaaatca tacagatagc ggtaatatcc
      421 gttcggcttc gccagcaatt cctgctgtgt tcccgcttcc acaatcctgc ctttatccat
      481 ggcaatgatc cggtgtgccg ttttaacagt ggacagacgg tgggcgataa tcagcaccgt
      541 ccggttggcg caaatggcct gcatgttctg cataatcgct cgttcacttt cataatccag
      601 cgcgctggtg gcttcatcaa aaatcagaat gcgcggattg gtgattaacg cgcgggcaat
      661 cgcaatacgc tgccgctgtc cgcccgacaa gccggcccct tgttcgccca ccacggtgcc
      721 gtagccttcc ggcagctcca taataaactc gtgtgcgccc gccagtttgg ctgcttcgat
      781 aatgcgttcc agcggcatac ccgtatccgt cagcgcgata ttgtcgcgta tgctgcggtt
      841 gagcagcaca ttctcctgca agaccacgcc gacctgccgc cgcagccagg caggagcggc
      901 caaagccaaa tcgttgccgt ccaccaacac ccgtccctgc tccggtacat acagacgctg
      961 caccaatttg gtgagtgtgg atttgcccga ccccgaacgt cccacaatcc ccagcacttc
     1021 ccccgcccga atccgcaggt tcaaatcctg caaaatcagc ctgccgtccg ccttatagcg
     1081 gaaatcgaca tgttcgaacg taatctcccc ccggatatcg ggcaaagcca aatgcgaaga
     1141 cgcattctcg gtcggcgcat tcagaatatc ccccaaacgc gccaccgaaa tccccacctg
     1201 ctggaaatcc tgccacaact gcgccaaacg gataacaggc gccgccacct gtcccgagag
     1261 catattaaac gcaatcagct gccccaccgt cagcttgccc tcaattacca gccgtgcgcc
     1321 aatccacaac gtcgccaccg tcaccagctt ctgaatcagc tgcaccccct gctggccgac
     1381 caccgccaac ttcgttaccc gaaatcccga agccacataa gccgccaact gattgtccca
     1441 acgctgcgtc atctgcggct ccaccgccat cgcctttacc gtacccaccg cagtgatgct
     1501 ttctactaaa aacgactggt tgtctgcatt gcgcgcgaac ttatcgttca gacgcgtccg
     1561 cagtatcgga ctgataaatg ccgaccaaaa cgcataggca ggcaacgaag ccaataccac
     1621 ccaagtcaga gtggagctgt aataccacat caccgccaga aagataaacg aaaacgccaa
     1681 atccaacacc gaagtcagcg cctgaccggt caagaaattg cgaatctgct ccaattcccg
     1741 cacccgagcc accgtatcac ccactcgtct gtgctcgaaa taggataaag gcagggaaag
     1801 cagatgccgg aacaaacgcg cgcccaattc cacatcaata cgtgaagtcg tatgtgcaaa
     1861 cagatacgtc cgcaaaccgc ccaacacaat ctcaaacagc gacaccacca acaaagccac
     1921 cgacaccaca tccaaagtag agaatccccg atgtaccagc accttgtcca tcaccacttg
     1981 gaaaaacaga ggcgtaatca gcgcaaacag ctgcaacacc accgacacca ccaatacttc
     2041 aaaaaacaac cggcggtatt tgattaccgc cggaataaac caggtaaagt caaactttgc
     2101 caaactgccc aataccgaag cgcgggaagc aaccaatatc agtttgcccg aatatctgtt
     2161 agaaaattcg gcaaaagaca ataccgcaga cttattcgta accaaatcct gtatcaaaaa
     2221 ttgggcatgc tcaccctcac cgtctgtttt ggccaaaatg aaatggttgc cgtcatcaca
     2281 ccataccaat gcgggtaaag tcgccatagc caaacgttta ataggctggc ggactacctt
     2341 tgccttcaat cccaaagatt tggcggctaa cagccattgc gtttcattta aatcgctctg
     2401 tgcggaagta caaaattcat gctgtatatc ggcaggattg gcggcaatgc cgtggtaatg
     2461 ggcgaggatg atgagggcgg aaagggcggg gagcggtgcg gatacgatag acataaataa
     2521 aatatagtta gattggatgt ggataacggc tggctggaaa aggaatatat taagtagaaa
     2581 gaaatatata aataaaacag cagaacgcat tgtaaggata tatatgggaa ttgtaaagag
     2641 aaagtatgga aaagttctcg tttcaggaag gtaaaacggc ttaggaatcg agttagatga
     2701 ggatgcctcg cacctctcgt gcctcctgca taccgttaag gcacagggtt aaggtgcagg
     2761 ctgctccgaa ctctgttgcg gtcgggtaat gttatttttt gtgtttcagg cagcctgaaa
     2821 tatctgtata tttttgtttt aaatagattt taaagattga taactgttct tgacgatttt
     2881 tcaagaaagg agtaaatttc aagaaaggag taaagtgact tattatcaat gacaagcaac
     2941 gcgcgaagtg acaaggaaaa ctatctactt aaattctaag gaggcttcga atatcataaa
     3001 ccaatcagaa acatagagat aaaaattatg tacaaatata atcctcttat acaatttatt
     3061 gcacagttga ttatgtctta tggagcaagc gtagggtggg cacttgctgc cccacgcgtt
     3121 tcatatttca aggcagcctg aaaccgtgtg ggcataaatg cctaccctac atcccaaaaa
     3181 acaagcgcag cctgcgtgtg tagggtgcga actttcggca ggtagacacg cggttttata
     3241 gtttcaagct gagttgggat gcttaagata aagtacaaaa ccttaaaaaa taagggggct
     3301 gtactagatt agccctaaat tccacaccaa tcccgcaaaa tttttagctg tcgggacggt
     3361 gtgccgaagt taaaccgaaa tt
//
LOCUS       DAWFAD010000173         3366 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWFAD010000173 DAWFAD010000000
VERSION     DAWFAD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.173"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..1177
                     /locus_tag="WGO49_001853"
     CDS             <1..1177
                     /locus_tag="WGO49_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0306916.1"
                     /translation="DIQARGYQYDTETDKFVKGYEYSNCLWYEDERRINRTYGCYGVD
                     SSIMRLMSDYSRFPEVKELMESQMERLARPYWEKLRNRPDMYYFKNYNFKRCYFGLNG
                     GDCLVAKGDDGRTFISFSLQGNSKYKEEMDAKKLEEILSLKVDANPDKYIKATGYPGY
                     SEKVEVAPGTKVNMGPVTDRNGNPVQVVATFGRDSQGNTTVDVQVIPRPDLTPGSAEA
                     PNAQPLPEVSPAENPANNPAPNENPGTRPNPEPDPDLNPDANPDTDGQPGTSPDSPAV
                     PDRPNGRDGKDGGLLCKFFPDILACDRLPEPNPAEDLNLPSETVNVEFKKSGIFQDSA
                     QCPAPVTFTITVLDSSKQFAFSFENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            1480..1764
                     /locus_tag="WGO49_001854"
     CDS             1480..1764
                     /locus_tag="WGO49_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ0306917.1"
                     /translation="MKLLAALIPLLMSVVGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     HSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            1765..2952
                     /locus_tag="WGO49_001855"
     CDS             1765..2952
                     /locus_tag="WGO49_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ0306918.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMANDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGSKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIILLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEHQAVFQDKTEGEPVNNGNLTADMFVPTLSE
                     KPESKPIYNGVRQVRTFEYIAGCVEGGRTGCTCYSHQGTALKEITKEMCKDYARNGLP
                     FNPYKEESQGRDVQQSEQHHSDRPQVATLGGKPWQNLMYDNWQERGKPFEGIGGGVVG
                     SAN"
     gene            3167..>3366
                     /locus_tag="WGO49_001856"
     CDS             3167..>3366
                     /locus_tag="WGO49_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0306919.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAA"
BASE COUNT         1048 a          757 c          813 g          748 t
ORIGIN      
        1 agacatacag gcacgaggct accaatacga caccgaaacc gacaaatttg taaaaggcta
       61 cgaatatagt aattgccttt ggtacgaaga cgaaagacgt attaatagaa cctatggctg
      121 ctacggcgtt gacagttcca ttatgcgcct tatgtccgat tacagcagat tccccgaagt
      181 caaagaattg atggaaagcc aaatggaaag gcttgcccgt ccgtattggg aaaagttaag
      241 gaatcgtcct gatatgtatt attttaaaaa ctacaatttt aaacgttgtt atttcggatt
      301 gaacggcgga gattgtttag ttgctaaagg tgatgatggt agaactttta tcagtttctc
      361 acttcaagga aattcaaaat acaaagaaga aatggatgcc aaaaagctgg aagagatttt
      421 atcgttgaaa gtcgatgcca atcccgacaa atacataaag gcaaccggat atcccggtta
      481 ttccgaaaaa gtagaagtcg cacccggaac aaaagtgaat atgggacccg tcacggacag
      541 gaacgggaat cccgttcagg ttgtcgcaac attcggcagg gattcgcaag gcaacaccac
      601 agtggatgtt caagtaattc cgcgtcccga cttaaccccc ggaagcgcgg aagcgccgaa
      661 cgcacagccg ctgcccgaag tatcgcccgc tgaaaacccc gcaaacaacc cggcccccaa
      721 tgagaacccc ggcacgcgtc ccaatcccga acccgacccc gatttgaatc ccgatgcaaa
      781 tcccgatacg gacggacagc ccggaacaag ccccgattcc ccggccgttc cggaccgccc
      841 aaacggcagg gacggcaaag atggcgggct tttgtgcaaa ttcttccccg acattctagc
      901 ttgcgacagg ctgcccgagc ccaatccggc agaagattta aatctgccgt ctgaaaccgt
      961 caatgtagag tttaagaaat ccggaatctt tcaagattcc gcacagtgtc ccgcccccgt
     1021 tacgttcact ataaccgtgc ttgattcaag caagcagttc gcgttcagct ttgagaacgc
     1081 atgtaccata gccgaacggc taaggtacat gcttctcgcc cttgcttggg cggttgccgc
     1141 ctttttttgt atccgcacag tatcccgtga agtctagcag gcgcagcacc gccgggcttc
     1201 agtaacttgt gccaaggcag ggggaggacg tccagaaaga tttgtaaaga cggctttatc
     1261 gtctttataa atctttttgg ataccccttg ccgccccgcc aaaagaacac actctgctgc
     1321 aagggcaggt ggtaaggcgc gcgctttttg cgccgtcccc ctgcccccgc agcgtcgcaa
     1381 gtgagactgg gggtgtgggg gctagtcccc gcaaaatctt tcagattaag aaacattttt
     1441 ttaatgaggc aaccgtgcct tttaagaaag ggatagcaaa tgaaattgtt ggccgcattg
     1501 attccgctct tgatgagcgt cgtaggccgt atattgactg cattgggatt gatggccgtg
     1561 acctattcgg gggtggatag attggtagcc cattttcaac aggcgataac ccatagcata
     1621 acgggcgcac ctcaagcaat gttacagctt ttctatataa gcggcggtgg tactgttcta
     1681 aacattcttt tcggcgcgat cgcctttatt ctgtcattca aacaaatgac aaaactagca
     1741 acctcaatcg ggaagaaaaa ataaatggca gagatctgtt tgataaccgg cacgcccggt
     1801 tcagggaaaa cattaaaaat ggtttccatg atggcaaacg atgaaatgtt taagccggat
     1861 gaaaacggca tacgccgtaa agtatttacg aacatcaaag gcttgaagat accgcacacc
     1921 tacatagaaa cggacgcgaa aaagctgccg aaatcgacag atgagcagct ttcggcgcat
     1981 gatatgtacg aatggataaa gaagcccgaa aatatcgggt ctattgtcat tgtagatgaa
     2041 gctcaagacg tatggccggc acgctcggca ggttcaaaaa tccctgaaaa tgtccaatgg
     2101 ctgaatacgc acagacatca gggcattgat atatttgttt tgactcaagg ctctaagctt
     2161 ctagatcaaa atcttagaac gcttgtacgg aaacattacc acatcgcttc aaacaagatg
     2221 ggtatgcgta cgcttttaga atggaaaata tgcgcggacg atcccgtaaa aatggcatca
     2281 agcgcattct ccagtatcta tacactggat aaaaaagttt atgacttgta cgaatcagcg
     2341 gaagttcata ccgtaaataa ggtcaagcgg tcaaaatggt tttatactct gccagtaata
     2401 atattgctga ttcccgtttt tgtcggcctg tcctataaaa tgttaagtag ttatggaaaa
     2461 aaacaggaag aacccgcagc acaagaatcg gcggcaacag aacatcaggc agtatttcag
     2521 gataaaacag aaggcgagcc ggtaaacaac ggtaacctta ccgcagatat gtttgttccg
     2581 acattgtccg aaaaacccga aagcaagccg atttataacg gtgtaaggca ggtaagaacc
     2641 tttgaatata tagcaggctg tgtagaaggc ggaagaaccg gatgcacatg ctattcgcat
     2701 caagggacgg cattgaaaga aattacaaag gaaatgtgca aggattacgc aagaaacgga
     2761 ttgccgttta acccatataa agaagaaagc caagggcggg atgtccagca aagtgagcag
     2821 caccattcgg acagaccgca agttgccacg ttgggcggaa agccgtggca aaatcttatg
     2881 tatgataatt ggcaggagcg cggaaaaccg tttgaaggaa tcggcggggg cgtggtcgga
     2941 tcggcaaact gaagaaaacg gcaagagaga aaaaagaccc gtaaaccgtt tgaatataga
     3001 cggcttacgg gtctttgttt cgcgcaaagc aagggctaag gcagtcaggc agcaaatccc
     3061 gcaatgtatt aaaacagacg cgtagaaatg ccggctgcct ttatccatcc tcaaaattga
     3121 atatcatcct agccgtatca aggctgtata aataaggaaa ataccaatga atataatcgg
     3181 gctggacatc tcaaaggata ccatagacgc aacattgcat aaaacaaacg gaagtatcca
     3241 ttacattaaa tttaagaata atgatgatgg attaaaacag tttagattgt ggataaaggg
     3301 aaacagaatc agaaaagcct atatcggcat ggaggcaaca ggcatctatt acgaaaaggc
     3361 agcaga
//
LOCUS       DAWFAD010000174         3355 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWFAD010000174 DAWFAD010000000
VERSION     DAWFAD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.174"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            187..1062
                     /locus_tag="WGO49_001857"
     CDS             187..1062
                     /locus_tag="WGO49_001857"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ0306920.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADFTLS
                     VVPYYNKPSQEGIYQHFKTIAEATSIPMIIYNVPGRTVVSMTNDTILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARKLNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            1071..2267
                     /gene="bamC"
                     /locus_tag="WGO49_001858"
     CDS             1071..2267
                     /gene="bamC"
                     /locus_tag="WGO49_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ0306921.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTLIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGSLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTVWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKNPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2324..3256)
                     /gene="pip"
                     /locus_tag="WGO49_001859"
     CDS             complement(2324..3256)
                     /gene="pip"
                     /locus_tag="WGO49_001859"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ0306922.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIQHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLADALVQAVEDIFPRLL"
BASE COUNT          881 a         1123 c          744 g          607 t
ORIGIN      
        1 ccgacggcac cggcataaac cggcgaatat aataaaatga tttcaaacag gaaaaatcaa
       61 tttccaccat acctttcggc gcaaacgccc cacatctgca acccgccgga tttcacacct
      121 gcggtaaagt aggctacaat cagaagcatt accgccccct acaccacaac tgataaaagg
      181 ttctgtatgt tacaaggtag cctggttgcc ctgattaccc cgatgaatca agacggcagc
      241 atccattacg aacaactccg cgatttgatc gactggcaca ttgaaaacgg cacggacggc
      301 atcgttgccg tcggcacgac aggcgaatcc gccaccctct ccgtcgaaga acacaccgcc
      361 gtcatcgaag ccgtcgtcaa acacgttgcc aaacgcgtcc ccgtcatcgc cggcacaggg
      421 gcaaacaaca ccgtcgaagc catcgccctt tcccaagccg ccgaaaaagc cggtgcagac
      481 ttcactctct ccgtcgtccc ctattacaac aagccctccc aagaaggcat ttaccaacat
      541 ttcaaaacca tcgccgaagc cacttcgatt ccgatgatta tctacaacgt gcccggccgt
      601 accgtcgtca gcatgactaa cgacactatc ctgcgccttg ccgaaatccc caacatcgtc
      661 ggcgtgaaag aagccagcgg caacatcggc agcaacatcg aactcatcaa ccgcgcgccc
      721 gaaggcttcg tcgtcctttc cggcgacgac cataccgccc tgccgttcat gctgtgcggc
      781 ggacacggcg tgattaccgt agccgccaac gccgcaccca agctctttgc cgatatgtgc
      841 cgcgccgcgc tgcaaggcga cattgccctc gcacgcaagc tcaatgaccg gctgattccg
      901 atttacgaca ccatgttctg cgaacccagc cccgccgcac cgaaatgggc ggtatccgcc
      961 ctgggcagat gcgaaccgca cgtccgcctg ccgctcgttc cgctgacgga aggcggacag
     1021 gcaaaagtcc gcgccgccct gaaagcctca ggacaactct aaatataact atgccgtctg
     1081 aaccgttcgg acggcataac gcaacaaaca ctttaatatc catcacacag gatgacacga
     1141 tgacccttat caaacccgtc attgccgcgc tcgcactcat cgggcttgcc gcctgctccg
     1201 gcagcaaaac cgaacagccc aagctcgact accaaagccg gtcgcaccgc ctgatcaaac
     1261 ttgaagtccc acccgatttg aacaaccccg accaaggcag cctctaccgc ctgcctgccg
     1321 gttcgggagc cgtccgcgcc agcgatttgg aaaaacgccg cacacccgcc gtccaacagc
     1381 ctgccgatgc cgaagtattg aaaagcgtca aaggtgtccg cctcgagcgc gacggcagcc
     1441 aacgttggct tgtcgttgac ggcaaatccc ctgccgaaat ctggccgctt ctgaaagcct
     1501 tttggcagga aaacggcttc gacatcaaat ccgaagaacc cgccatcgga caaatggaaa
     1561 ccgagtgggc ggaaaaccgt gccaaaatcc cccaagacag cttgcgccgc ctattcgaca
     1621 cagtcggttt gggcggcatc tactccaccg gcgagcgcga caaattcatc gtccgtatcg
     1681 aacagggcaa aaacggcgtt tccgacatct tcttcgccca caaagccatg aaagaagtgt
     1741 acggcggcaa agacaaagac acgaccgtat ggcagccctc cccgtccgat cccaacctcg
     1801 aagccgcttt cctgacgcgc tttatgcaat atttgggcgt tgacggacag caggcggaaa
     1861 acgcatcggc aaaaaacccg accctgcccg ccgccaacga aatggcgcgt atcgaaggca
     1921 aaagcctgat tgtctttggc gactacggca gaaactggcg gcgcaccgcg ctcgccctcg
     1981 accgcatcgg gctgaccgtc gtcggtcaaa acaccgaacg ccacgctttc ctggttcaaa
     2041 aagccccgaa cgaaagcaat gcagttaccg aacaaaaacc cggcctgttc aaacgcctgc
     2101 tgggcaaagg caaagcggag aaacctgccg aacagccgga actgattgtc tatgccgagc
     2161 ctgtcgccaa cggctcgcgc atcgtcctgc tcaacaaaga cggcagcgca tatgccggca
     2221 aagacgcatc cgcattattg ggcaaactcc attccgaact gcgttaaacc attcaaaaca
     2281 aaagcagctt ccaaacagaa gctgcttttt tatgcggaac tttttacaac aaccgaggaa
     2341 aaatatcctc aaccgcctga accaacgcat ccgccaaagg cggatcgaac gcacgatgcc
     2401 ctgcctgaac caccctcaat tccgcttccg gaaaggcttt cgacagcgcc cacgcactct
     2461 gcatcggcgt acacaaatca taccgcccct gcacgataat ggtcgggata tgctgtattt
     2521 tgccgatatt gttcaaaatc gccctatcgc cctgcaacca gccgccgttg acaaaataat
     2581 ggttttccaa acgcgcgatt gccagcgagg cataagcatc ttcatccact tcctccggct
     2641 cgaaacggat cagatagctt tcccaatccg cccaagcctt tgcggcagac aggcacactt
     2701 cttcatcttg atgaaacagc aacccgtgat acgcctcaat cagccggttc cgccgatttt
     2761 cagcaatcgg cgcgacaaat ttttgccatt gttccggata aatccggctc acaccgcccg
     2821 cctcgttcag ccacgccgtt tcagacggcc tgcacaaaaa tatcccgcgc aacaccaatc
     2881 cctttacccg ttccggatgg gtttgggcat aagccagcga caaagtactg ccccacgaac
     2941 cgccgaacac cagccatttc ccgataccca gcatttcacg gactttttca atatccgcca
     3001 ccaaatccca agtcgtattg tcttccgcac aagcatacgg gcgcgaacgt ccgcaaccgc
     3061 gctggtcaat aatgacgatg cggaacacat cgggattgaa aaaaccccga cattcaggcg
     3121 aagcccccgc ccccggtccg ccgtgtaaaa aaatgaccgg cacaccgtcg ggattgcccg
     3181 actcttccca ataaatttga tgaatttcag acacctgcaa gtatccgcta tgaaaaggct
     3241 gttttatctc atacatcatc ttctcccata ttgcctctga caatccgaac attcccacac
     3301 gcaacatcac gcacgaaaca caagccgccc cgaaaggcaa attccgaaaa atcgc
//
LOCUS       DAWFAD010000175         3352 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWFAD010000175 DAWFAD010000000
VERSION     DAWFAD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.175"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(6..884)
                     /locus_tag="WGO49_001860"
                     /pseudo
     CDS             complement(6..884)
                     /locus_tag="WGO49_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1655 family transposase"
     gene            complement(1096..1209)
                     /locus_tag="WGO49_001861"
                     /pseudo
     CDS             complement(1096..1209)
                     /locus_tag="WGO49_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            1349..1424
                     /locus_tag="WGO49_001862"
     tRNA            1349..1424
                     /locus_tag="WGO49_001862"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1383..1385,aa:Glu,seq:ttc)
     gene            1809..1974
                     /locus_tag="WGO49_001863"
                     /pseudo
     CDS             1809..1974
                     /locus_tag="WGO49_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
     gene            complement(2024..2830)
                     /gene="thiD"
                     /locus_tag="WGO49_001864"
     CDS             complement(2024..2830)
                     /gene="thiD"
                     /locus_tag="WGO49_001864"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ0306923.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGSDVCKAVRTAKSYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          746 a          822 c          886 g          898 t
ORIGIN      
        1 caacactagt gtattagtgg ttggaacaga ttcaagaata aaacacttgg cgtttcgtag
       61 ccaagtgttt ttcttggtcg gtggttcaac tcatcttgaa ccctgcgtat ctcccgatca
      121 ctgatgttac ggaaatcggt ttgtttgggg aagtattgcc ggatgagtcc gttggtgttc
      181 tcattcagcc ctttctccca agaatggtaa gggcgacaaa aataagtctc cgctttcaat
      241 gctttggtta ttttggtgtg ttggtagaac tctttgccgt tatccatggt aatggtgtgc
      301 accctgtctt tatgtgcctt taatgcccta acagctgccc gggcagtgtc ttcggctttg
      361 aggctatcca atttgcagat gatggtgtag cgggtaacgc gttcgaccaa ggtcaataat
      421 gcgcttttct gtcctttgcc gacaatggtg tcggcttccc aatcgccgat acgggatttc
      481 tggtcgacga tagcgggtcg gttttctatg ccgacacggt tggggacttt gcctctggtc
      541 catgtgctgc cgtagcgttt gcggtagggt ttgctgcata ttctgagatg ttgccacaac
      601 gtgctgccgt tgcttttgtc ttggcgaagg tagcggtaaa tggtgctgtg gtggagcgtg
      661 atctggtggt gtttgcacag gtaggcgcat acttgttcgg gactgagttt gcggcggata
      721 agggtgtcga tgtgctgaat cagctgcgaa tcgagcttat agggttgtcg cttacgctgt
      781 ttgatagtcc ggctttgccg ctgggctttt tcggcgctgt attgctgccc ttgggtgcgg
      841 tgccgtctga tttcgcggct gatggtgctt ttgtggcggt tcaaaagttg cacttcaaat
      901 gcgaatccgc cattatctga aagtttgagg ttctagattc ccgctttcgc gggaatgacg
      961 aaaagtggcg ggaatgacga agtttcagac ggcatcgccc gcctgttttg atatggcggc
     1021 accccccccc gacaaaaaaa aacaatccgg aacgcatctg accgttccgg cttgttttca
     1081 ggcgaatccg ccgcatcaga acatactgcg cacgcccata ttgacctgcc aagtctggcg
     1141 catcgtgtgc atcgaagacc tttgcgcctc aaaataaagc tgccttccgt tgtcggcatt
     1201 accacgcaaa aaaatgaatt gcttgatatt ccaatgtttt ttatatgttt ttatattgtg
     1261 atgcgatcag acaaacgccc ccctgacatt tgtttagacg gcatcgtatt gctaaatttc
     1321 tataagtatg tataatgtcc gtttccacgc gcccatcgtc tagaggccta ggacactgcc
     1381 ctttcacggc ggcaaccggg gttcgaatcc ccgtgggcgt gccaattcaa aaacctgctt
     1441 gtttcaagca ggttttttta ttatgaatcg tcattcccgc acccttcgtc attcccgcga
     1501 aagcgggaat ctggagcgca ggattaaaga aaccgtttta tccgataagc ttccgtgccg
     1561 acaggtctgg attcccgcct gcgcgggaag gacggcagag ggtggacgat gccgtctaaa
     1621 gcctgacaag gcatttgatg ccgtctgaaa cttcgtcatt cccgcaccct tcgtcattcc
     1681 cgcaaaagcg ggaatctaga gcgtagggtt gaagaaaccg ttttatccaa taagtttccg
     1741 tgccgacagg tctggattcc cactttcgtg ggaatgacga gtcttttata acctgagacc
     1801 tttgcaaaat gcctttccct tttaaattta aagagggggg agctgaagga ttattatcgg
     1861 gattgggagc tggtcgaata taaagaagaa ttgggcgcga tgcacgccaa agacgaaaac
     1921 ggcaatccga tacggtttaa atttgtaacc atgctggcga aaaagcctga ataatcctca
     1981 aaaatgccgt ctgaacattt tgtttcagac ggcattttta cgtctagtcc cgatacgccc
     2041 aatgattgac cgggccgtgt cccgcgccga tttccaaagg gtttgagatt gccgccgtaa
     2101 tgtaggactt ggcagtccgt acggctttgc aaacgtccga gccttttgcc aactcggcgg
     2161 tgatgcaggc ggaaaacgtg cagcccgtgc cgtgcgtgtg ggcggttgga aagcgcgggc
     2221 tgtccaattc cagcgtttca ttttgcgtaa acagccaatc cgtgcagcgt ccgcttgtgc
     2281 tgccgttcaa atgtccgcct ttgataacga catttttgac accgtaatca agcaggattt
     2341 ttgccgcacg ttccgcatct ttacggtttt cgatatgcac gccggtcagg gcttccgcct
     2401 cgggcaggtt gggggtcaat acatccgtat cgggaagcag caggcgcgtc agtgccgcaa
     2461 cggcggaatc ctgcaacagc ggcgcaccgc ctttggcaat catcacaggg tcgagtacgc
     2521 gcctgccgaa gtcgcagtgt ttcagcttgt cggcaacgca ttcgatgatt tccgccgtgc
     2581 cgagcattcc tattttgtag gcgcggatgt caaagtcttc cctgattgct tggatttgtg
     2641 cggtgatggt ttcggtcggg acgagatgaa ccgccgacac gcccaaggta ttttgcgcgg
     2701 taacggcggt gatgacgcac gttccgaaca cgccgcgcat ctgaaacgtt ttcaaatccg
     2761 cctgaatgcc cgcaccgccg cccgaatccg aaccggcgat ggtaagcgtt tgcacaaaag
     2821 agcttttcat ttgttcctcc ttaatattgt gtccgataaa aagggctaat gcctgtaaaa
     2881 aagcacggcg atgccgtctg aaaaccgcag cgcggggttt gtggtgcatt atatgccgta
     2941 ttgcaggaca aggcacaggg cagggcggcg gggtgcatcg tagcaactgt atttttgccc
     3001 gacggggtga aaaatacagt tgctacaccc actgataatg aaaaaagaga gcaatgccgt
     3061 ctgaaccgcg tgttgcggcg agcataaacc ggcacgcttt attcgtcatt cccacaaaaa
     3121 cagaaaccta aaattccgtc attcctacga aagcgggaat ccagtgtgtt cggtttcagt
     3181 tgtttttggg tttcgggtga tttccaaatc gaaacctttg caaaattccc caaaatcccc
     3241 taaattccca ccaagacatt taggggatgt tccatgagca ccttctttca acaaaccgca
     3301 caagccatga ttgccaaaca catcgaccgt ttcccgctat tgaagttgga cc
//
LOCUS       DAWFAD010000176         3343 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWFAD010000176 DAWFAD010000000
VERSION     DAWFAD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.176"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            60..689
                     /locus_tag="WGO49_001865"
     CDS             60..689
                     /locus_tag="WGO49_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ0306924.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKELPYE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALGLSPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            864..3242
                     /gene="hmbR"
                     /locus_tag="WGO49_001866"
     CDS             864..3242
                     /gene="hmbR"
                     /locus_tag="WGO49_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent hemoglobin receptor HmbR"
                     /protein_id="HEZ0306925.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVFAADEAATETTPVKAEVKAVRVK
                     GQRNAPAAVERVNLNRIKQEMIRDNKDLVRYSTDVGLSDSGRHQKGFAVRGVEGNRVG
                     VSIDGVNLPDSEENSLYARYGNFNSSRLSIDPELVRNIDIVKGADSFNTGSGALGGGV
                     NYQTLQGRDLLLPERQFGVMMKNGYSTRNREWTNTLGFGVSNDRVDAALLYSQRRGHE
                     TESAGKRGYPVEGAGSGANIRGSARGIPDPSQHKYHSFLGKIAYQINDNHRIGASLNG
                     QQGHNYTVEESYNLLASYWREADDVNRRRNTNLFYEWTPESDRLSMVKADVDYQKTKV
                     SAVNYKGSFPIEDSSTLTRNYNQKDLDEIYNRSMDTRFKRITLRLDSHPLQLGGGRHR
                     LSFKTFASRRDFENLNRDDYYFSGRVVRTTSSIQHPVKTTNYGFSLSDQIQWNDVFSS
                     RAGIRYDHTKMTPQELNAECHACDKTPPAANTYKGWSGFVGLAAQLNQAWRVGYDITS
                     GYRVPNASEVYFTYNHGSGNWLPNPNLKAERSTTHTLSLQGRSEKGTLDANLYQSNYR
                     NFLSEEQKLTTSGDVSCTQMNYYYGMCSNPYSEKLEWQMQNIDKARIRGIELTGRLNV
                     DKVASFVPEGWKLFGSLGYAKSKLSGDNSLLSTQPLKVIAGIDYESPSEKWGVFSRLT
                     YLGAKKVKDAQYTVYENKGWGTPLQKKVKDYPWLNKSAYVFDMYGFYKPVKNLTLRAG
                     VYNVFNRKYTTWDSLRGLYSYSTTNSVDRDGKGLDRYRAPSRNYAVSLEWKF"
BASE COUNT          898 a          876 c          828 g          741 t
ORIGIN      
        1 gggcaagtgc ggattttgcc cgccccgcat ccttatccct taccgacaaa ggaaaactta
       61 tgagtgaaac cgaaaatcaa gccctgacgt ttgccaaacg tctgaaggcg gataccacgg
      121 cggttcacga cagcgtggat aacctcgtta tgtctgtcca accgtttgtc agcaaagaaa
      181 actacatcaa atttttgaaa ctccaatccg ttttccacaa ggccgtcgac cacatctata
      241 aagatgccga attaaacaaa gccattcccg agctggaata catggcgcga tacgatgccg
      301 taacgcaaga cttgaaagat ttgggcgaag agccttacaa attcgacaaa gaattgccat
      361 acgaaactgg caacaaagca atcggctggc tttattgcgc cgaaggatcc aatttgggcg
      421 cggcgttttt gttcaaacac gcccaaaaac tcgattacaa cggcgaacac ggcgcgcgcc
      481 acctcgctcc gcatcccgac gggcgcggca aacactggcg cgccttcgtc gagcatctga
      541 acgcattggg cttaagcccc gaagccgaag ccgaagccat ccaaggcgcg cgcgaagcct
      601 ttgcattcta caaagtcgtg ttgcgcgaaa ccttcggctt ggcagccgat gccgaagccc
      661 ccgaaggtat gatgccgcac aggcactaaa aaataatcga accaaataaa caaggtctcg
      721 gcatagctgt ttgcagggac ctttaattac acggcgcggc tttgtttaca tggattactg
      781 tcttattaaa tattaatgat tatcataaaa tctattattc gctaaccgat ggatgaacaa
      841 tccatacatc ttgagttgat aatatgaaac cattacaaat gctccctatc gccgcgctgg
      901 tcggcagtat tttcggcaat ccggtctttg cggcagatga agctgcaact gaaaccacac
      961 ccgttaaggc agaggtaaaa gcagtgcgcg ttaaaggcca gcgcaatgcg cctgcggctg
     1021 tggaacgcgt caaccttaac cgtatcaaac aagaaatgat acgcgacaac aaagacttgg
     1081 tgcgctattc caccgatgtc ggcttgagcg acagcggccg ccatcaaaaa ggctttgctg
     1141 ttcgcggcgt ggaaggcaac cgtgtcggcg tgagcataga cggcgtaaac ctgcctgatt
     1201 ccgaagaaaa ctcgctgtac gcccgttatg gcaacttcaa cagctcgcgt ctgtctatcg
     1261 accccgaact cgtgcgcaac atcgacatcg taaaaggggc ggactctttc aataccggca
     1321 gcggcgcctt gggcggcggt gtgaattacc aaaccctgca aggacgtgac ttactgttgc
     1381 ctgaacggca gttcggcgtg atgatgaaaa acggttacag cacgcgtaac cgtgaatgga
     1441 caaataccct cggtttcggc gtgagcaacg accgcgtgga tgccgctttg ctgtattcgc
     1501 aacggcgcgg ccatgaaact gaaagcgcag gcaagcgtgg ttatccggta gagggtgctg
     1561 gtagcggagc gaatatccgt ggttctgcgc gcggtattcc tgatccgtcc caacacaaat
     1621 accacagctt cttgggtaag attgcttatc aaatcaacga caaccaccgc atcggcgcat
     1681 cgctcaacgg tcagcagggg cataattaca cggttgaaga gtcttacaac ctgcttgctt
     1741 cttattggcg tgaagctgac gatgtcaaca gacggcgtaa caccaacctc ttttacgaat
     1801 ggacgccgga atccgaccgg ttgtctatgg taaaagcgga tgtcgattat caaaaaacca
     1861 aagtatctgc ggtcaactac aaaggttcgt tcccgataga ggattcttcc accttgacac
     1921 gtaactacaa tcaaaaggac ttggatgaaa tctacaaccg cagtatggat acccgcttca
     1981 aacgcattac cctgcgtttg gacagccatc cgttgcaact cggggggggg cgacaccgcc
     2041 tgtcgtttaa aactttcgcc agccgccgtg attttgaaaa cctaaaccgc gacgattatt
     2101 acttcagcgg ccgtgttgtt cgaaccacca gcagtatcca gcatccggtg aaaaccacca
     2161 actacggttt ctcactgtct gaccaaattc aatggaacga cgtgttcagt agccgcgcag
     2221 gtatccgtta cgatcatacc aaaatgacgc ctcaggaatt gaatgccgag tgtcatgctt
     2281 gtgacaaaac accgcctgca gccaacactt ataaaggctg gagcggtttt gtcggcttgg
     2341 cggcgcaact gaatcaggct tggcgtgtcg gttacgacat tacttccggc taccgtgtcc
     2401 ccaatgcgtc cgaagtgtat ttcacttaca accacggttc gggtaattgg ctgcccaatc
     2461 ccaacctgaa agccgagcgc agcaccaccc acaccctctc tctgcaaggc cgcagcgaaa
     2521 aaggtacttt ggatgccaac ctgtatcaaa gcaattaccg caatttcctg tctgaagagc
     2581 agaagctgac caccagcggc gatgtcagct gtactcagat gaattactac tacggtatgt
     2641 gtagcaatcc ttattccgaa aaactggaat ggcagatgca aaatatcgac aaggccagaa
     2701 tccgcggtat cgagctgacg ggccgtctga atgtggacaa agtagcgtct tttgttcctg
     2761 agggctggaa actgttcggc tcgctgggtt atgcgaaaag caaactgtcg ggcgacaaca
     2821 gcctgctgtc cacccagccg ttgaaagtga ttgccggtat cgactatgaa agtccgagcg
     2881 aaaaatgggg cgtgttctcc cgcctgacct atctgggcgc gaaaaaggtc aaagacgcgc
     2941 aatacaccgt ttatgaaaac aagggctggg gtacgccttt gcagaaaaag gtaaaagatt
     3001 acccgtggct gaacaagtcg gcttatgtgt tcgatatgta cggcttctac aaaccggtga
     3061 aaaacctgac tttgcgtgca ggcgtatata atgtgttcaa ccgcaaatac accacttggg
     3121 attccctgcg cggcctgtat agctacagca ccaccaactc ggtcgaccgc gatggcaaag
     3181 gcttagaccg ctaccgcgcc ccaagccgta attacgccgt atcgctggaa tggaagtttt
     3241 aatctggtat tattgaatta atcgccttgt tgaaaattaa agccgtccga attgtgttca
     3301 agaactcatt cggacggttt tttaccgtaa atctgtgtga tgg
//
LOCUS       DAWFAD010000177         3330 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWFAD010000177 DAWFAD010000000
VERSION     DAWFAD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.177"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            238..1197
                     /locus_tag="WGO49_001867"
     CDS             238..1197
                     /locus_tag="WGO49_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306926.1"
                     /translation="MYQNYDIVILSLGNGLLTRFFRNSSKLNLTLPIVITLFPGVIFG
                     DSESIITRLGANILLFNNYYDYQIGNKFKERYNLSCTNILYGYPILRKKHHCSDGNKV
                     YFIDQVKIPDGKKERLLILKKLIALANRYPHKDLVLLLRVHQNDITVHEDKYSYAELA
                     KNMKLPDNFKIERKRTQDALQEMGYCLSFSSTMLFEAESCGIPIGVISDLGVDKKYAN
                     THFKDSGVLVNLDTVDLDSPNTINPTWAELYTNPDSLSLKEFNQLLEVTSTQPPSFDN
                     YFIAVNDVQPVSMVLLRKFKKLIYHPKQFFVDSKLYKLIKFTK"
     gene            1245..2108
                     /gene="galU"
                     /locus_tag="WGO49_001868"
     CDS             1245..2108
                     /gene="galU"
                     /locus_tag="WGO49_001868"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0306927.1"
                     /translation="MQKIRKAVFPVAGMGARFLPATKASAKEMLPIVDKPLIQYAAEE
                     AVAAGCTELIFITGRNKRNIEDHFDKAYELETELEMREKHKLLGYVQKILPDHVSCIF
                     IRQAEALGLGHAVLCARPAVGNEPFAVILADDLIDAPQGALKQMVDIYNQSGNSILGV
                     ETVDPSQTGSYGIVEVEQLKSYQRVTNIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGGEIQLTDGIARLLDHEFVLAHAFEGKRYDCGSKLGYLEATVAYGLKHPELK
                     DDFKELLKQFV"
     gene            2191..3207
                     /gene="galE"
                     /locus_tag="WGO49_001869"
     CDS             2191..3207
                     /gene="galE"
                     /locus_tag="WGO49_001869"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0306928.1"
                     /translation="MNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPRL
                     KTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPIKYYDNNVS
                     GSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERIL
                     TDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAVF
                     GDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRAF
                     EAASGLTIPYEVKPRRAGDLACFYADPAYTKTQTGWETKRDLAQMMEDSWRWVSNNPN
                     GYDD"
BASE COUNT          994 a          621 c          708 g         1007 t
ORIGIN      
        1 aacttaatat ctaatattta aaatacaatg aataaaaaac gagtcctatt aattgctagt
       61 tatgattctt ttttgaatac tggatatgct gttgcaaaag aaattaacta ttcaacaatc
      121 gatatttata tccaccaaac aaaaaaaaat attctttcgg aatcacaatt aagtttaaat
      181 gggctttctt taaaagaaat caattttttt catattgatg attatttcga caaattaatg
      241 taccagaatt atgatattgt tattctatca ttaggtaatg gtttactgac gcgttttttt
      301 cggaatagta gcaaacttaa tttaacatta cctatagtaa ttactctatt tccaggggta
      361 atttttggtg attcagaaag tattataact aggttaggtg ctaatatttt attgttcaat
      421 aattattatg attaccaaat aggcaataag ttcaaagaac gttacaactt atcgtgtaca
      481 aatattttat atggttatcc tatcctacga aaaaaacacc attgctctga tggcaataaa
      541 gtttatttca ttgatcaagt aaaaattcca gatggaaaaa aagaaagatt attaattcta
      601 aaaaaattaa tagcactggc taatcgctat ccacataaag atttagtact attattacgt
      661 gtccatcaaa atgatatcac agtacatgaa gataaatact cttatgctga attagcaaaa
      721 aatatgaaat tgcctgataa ttttaaaata gagagaaaac ggacacaaga tgccttacaa
      781 gaaatgggat attgtttatc attctcttca acaatgttat ttgaagctga aagctgcgga
      841 attcctattg gtgtcattag tgatttaggg gttgacaaga aatatgccaa tacacatttc
      901 aaagatagtg gtgttttggt taatttagat acggtagact tggactctcc aaataccatt
      961 aatccaactt gggctgaact atatactaat ccagatagcc tctctttaaa agagtttaac
     1021 caattattag aggttacttc tactcaacca cctagttttg ataattattt tattgcagtt
     1081 aatgacgttc agcctgtatc tatggttctg ttacgtaaat ttaaaaagct tatatatcat
     1141 cctaagcaat tctttgttga ttcaaaacta tataaattaa ttaaatttac taaataattt
     1201 ttactatggg ttgccttatt tatttcaaag gagtgacata aaaaatgcaa aaaattcgta
     1261 aagctgtatt ccctgtagct ggcatgggag cccgattctt acctgccacg aaagccagcg
     1321 ctaaagaaat gctgccaatc gtggacaaac cgttaattca atatgctgcg gaagaagccg
     1381 ttgccgcggg ttgtactgaa ttgattttta tcaccggccg taataagcgt aatattgaag
     1441 accacttcga taaagcctat gagttggaaa ctgaactgga aatgcgtgaa aagcataaat
     1501 tgcttggcta tgtgcaaaaa attctgcctg atcatgtcag ctgtattttc attcgtcagg
     1561 cagaagcttt gggcttgggt catgcggtat tatgcgcccg tcctgctgtc ggtaatgagc
     1621 cttttgccgt gattttggcc gatgacttga ttgatgcacc acaaggcgca ctcaagcaga
     1681 tggtagatat ttacaatcaa agcggcaaca gcattttagg ggtagaaaca gtcgatcctt
     1741 cgcaaaccgg ttcatacggc attgtcgaag tggaacagct gaaaagctat cagcgtgtaa
     1801 ccaatattgt ggaaaaacca aagcctgaag atgcgccgtc aaatttagcg gtagtcggcc
     1861 gttatatttt gacaccgcgt atttttgatt tgctgactaa tctacctcgt ggtgcaggtg
     1921 gtgaaatcca gctaactgat ggtattgccc gcctacttga ccatgaattt gtcttggcgc
     1981 atgcgtttga aggtaagcgt tatgattgtg gcagtaagtt gggctatctg gaagccacgg
     2041 tagcttatgg cttgaagcat cccgaattaa aagatgattt caaagaatta ttgaagcaat
     2101 ttgtataatt ttaaggccgt ctgaaaaaga aaaggcatca tgttttttac actttcaggc
     2161 ggccttttaa acataaagaa ggaaaacgtg atgaacattc tcgttaccgg cggcaccggt
     2221 tttatcggct cgcacaccgt tgtttctttg ctgaaaagcg gtcatcaagt cgtgattttg
     2281 gataacctat gcaattccag catcaatatc ctgccacgct tgaaaacgat taccggccaa
     2341 gagattccgt tttatcaagg cgatatccgc gatcgtgaga ttttgcgccg tatttttgcg
     2401 gaaaaccgca ttgattcggt gattcatttt gccggcttga aagcggtggg tgaaagtgtg
     2461 gccgagccga taaaatatta tgataataat gtttccggca gcttggtgtt ggcggaagaa
     2521 atggcgcgtg cgggcgtgtt tagcattgtg ttcagttctt cggcgacggt ttatggcgat
     2581 ccgggcaaag tgccttatac cgaggatatg ccaccgggcg acaccaccag cccttacggc
     2641 gcatcgaaat cgatggttga gcgcattctc actgacattc aaaaagccga tccgcgctgg
     2701 agcatgattt tgttgcgtta tttcaatccg attggcgcgc atgaaagcgg tttgattggc
     2761 gagcagccaa acggtatccc aaataatttg ttgccttata tctgccaagt ggcggcaggc
     2821 aaactgccgc aattagcggt attcggtgat gactacccta cccctgatgg cacgggtatg
     2881 cgcgactata ttcatgtgat ggatttggca gaaggccatg tcgcggctat gcaggcgaaa
     2941 agcaatgtag caggcacgca tttgttgaac ttaggttccg gccgcgcttc ttcggtgttg
     3001 gaaatcatcc gcgcatttga agcagcttcg ggtttgacga ttccgtatga agtcaaaccg
     3061 cgccgtgccg gtgatttggc gtgcttctat gccgaccctg cctacaccaa aacgcagacc
     3121 ggttgggaaa ccaagcgtga tttggcgcag atgatggaag actcatggcg ttgggtcagc
     3181 aacaacccaa atggctacga cgattaagtt gacctgatac aggccgtctg aaagagatgt
     3241 tttcagacgg cctctttatc tgaaaaacac acattctgtc tgctataatc tgtttatatt
     3301 ttttggctat cctctgaaat ttatgagaaa
//
LOCUS       DAWFAD010000178         3288 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWFAD010000178 DAWFAD010000000
VERSION     DAWFAD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.178"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(96..320)
                     /locus_tag="WGO49_001870"
     CDS             complement(96..320)
                     /locus_tag="WGO49_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306929.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLIIESEKNGRY"
     gene            423..2573
                     /gene="dinG"
                     /locus_tag="WGO49_001871"
     CDS             423..2573
                     /gene="dinG"
                     /locus_tag="WGO49_001871"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ0306930.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELTFALAKGRGRYLCPYKLYQLTQNNAQQNLLGFEAPEVLWD
                     SKPKPEELKLLHDIADEFSARRFNGDRDTWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAVAVRSLLKHVIGLNDALS
                     AARREKEQDGALLDRLTGDFGIFIARIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPGEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRVKTQRYGQQLLAGLPPFKRIG"
     gene            2609..3223
                     /locus_tag="WGO49_001872"
     CDS             2609..3223
                     /locus_tag="WGO49_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ0306931.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGAPVAGAASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIATAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT          824 a         1080 c          807 g          577 t
ORIGIN      
        1 gcacatcctg tcgccggtat tccctaagcg gtacggcaac ctaaaacaaa tgccgtctga
       61 acttccgttt cagacggcat ttttatacgg actactcaat atcttccgtt tttttcactt
      121 tcaataatca gatatgccca aatcaccgcc acccaagccg acaccgttcc catcgccggc
      181 aaaaaatgct gccaatccgt catcggcggc acagggcaac ggccgcattc gatttccgcg
      241 ccgcccaaca accggtacag caacatatcc gcaatccaaa gcaacaacgg gtatttcaaa
      301 cgctgcaacc aaaaaaccaa ccacgtccgc acgattcacc tcacccccca ttccgacaac
      361 gatattgtac cgcttccgtg caatgttaaa ataagcccac ttctaccgcc gtacaaaacg
      421 ccatgctcac cgatttagaa aaaaacgcca tccgcgacca ttaccaaaac atcggcaaaa
      481 acctgcccgg tttccgtccg cgcgcttcgc agcgggaaat gattgcggcg gttgccaacg
      541 ctttttcgcg gacgttggcg cgcgaagaag gcggcgagcc gcccaagcgc gagggcgaga
      601 gcatcgccgt aatcgaaggg ccgaccggcg tgggcaaatc gcttgcctac ctgctggcgg
      661 gcggcatcat ggcgcagacg cgcggcaagc ggctgattgt gagcagcgca acggttgcct
      721 tacaggagca gttggttgac cgcgacctgc cgtttctggt cgaaaaaagc ggtttggaac
      781 tgaccttcgc gcttgccaaa gggcgcggcc gctatctctg cccttacaaa ctctaccaac
      841 tgacgcaaaa caacgcccag caaaacctgc ttggttttga agcaccggaa gtattgtggg
      901 acagcaaacc caagcccgaa gaattgaagc tgctgcacga cattgccgac gaattttccg
      961 cccgacggtt caacggcgac cgcgacactt ggccggaaaa aatcgatgac gcgatttggc
     1021 tcaaagttac caacgaccgc cacggctgcc tgaaaaccgc ctgtcccaac cgtccggaat
     1081 gtccgtttta cctggcacgc gatgtcttgg aaaccgtcga tgtcgtcgtt gccaaccacg
     1141 accttctgct tgccgacatc agtatgggcg gcggcgtgat tctgcccgcg cccgaaaaca
     1201 gtttctattg catagacgaa gcgcaccacc tgcccaaaaa agccctcagc cgttttgccg
     1261 ccgaacattc gtggaatatt gccgtttgga cgctggaaaa actgccgcag ctgaccggca
     1321 agattgccgc gcttaccgat aaagccgaac ttgccaacct tgccgatgaa gccgccgcat
     1381 ccttgctcga cagcctgcac gaatggcagt tccatttggc ggaagagccg tctttgagct
     1441 tggggccgtc tgaaaacgac aggcgaacca acagcgaacc gacttggctg tgggaagacg
     1501 gcaaaatccc cgaaggcctc gaaaccaccg tttccaacac cgccgttgct gtgcgcagcc
     1561 tgctcaaaca cgttatcggg ctgaacgatg cgctttctgc cgcacgccgc gaaaaagaac
     1621 aggacggcgc gctcctcgac cgcctgaccg gcgatttcgg catcttcatc gcccgtatcg
     1681 aacaaatcag cgcggtttgg gatttgctct ccaccgtccc cctcgaaggc gaagagccgc
     1741 tggcaaaatg gataacccgc cgcgccgacg acaaaaacga ctacattttc aacgccagcc
     1801 ccatcagcag cgcgtcccac ctcgccaaca gcctgtggcg gcgcgcggca ggcgcggtat
     1861 tgacctccgc caccctgcaa tccttgggca actttaacct gatgctgcgc caaaccgggc
     1921 tgcaatggct gcccgagacc accaccctcg ccctcaaaag cccctttgac tttgaaaaac
     1981 agggcgaact ctacatcccc cccatatacg ccagccccaa agaccccgaa gcccacaccg
     2041 ccgccatcat cgagtggctg cccaagctca tttcgcccgg cgaagccatc ggcacgctcg
     2101 tcttgttttc ctcgcgcaaa caaatgcagg atgtcgccct gcgcctgccc ggagactacc
     2161 tgccgctctt gctcgtacaa ggcgaattac ccaaagccgt tctcctgcaa aaacaccacc
     2221 gggccataga agaaggcaaa gccagcatca tcttcggact cgacagcttt gccgaaggac
     2281 tcgacctgcc cggcaccgcc tgcgtgcaag tcatcatcgc caaacttccc ttcgccatgc
     2341 ccgacaaccc catcgaaaaa acccaaaacc gctggataga acagcgcggc ggcaaccctt
     2401 tcatcgaaat caccgtcccc gaagccggca tcaaactcat ccaggccgtc ggccgcctca
     2461 tccgcaccga acaagactac ggccgcgtaa ccatcctcga caaccgcgtc aaaacacagc
     2521 ggtacggcca acaattatta gccggcctgc caccgtttaa aaggataggg taaacctacc
     2581 gccctgcccg caacagaaag gaaaaatcat gaacttcacc cgactgctca accaagtctt
     2641 aagcacggtt caaaaaaaag gcaacacctt ctccgacagc ccgctcaatt cattcggcgg
     2701 aggcgcgccg gttgccggtg ccgcgtccat gctgctgaac ggtaaaaacc gcaaaaccat
     2761 taccaaaatc ggttcgaccg ccgctttggg ctacctcgcc taccggggct atcagatgtg
     2821 gcagcaaaac aaagggcggg caaccgtaac acaaagcgat ttccaacctg ccggagaaac
     2881 tgaagaaaca tacagccgca ccgtattgcg caccatgata gccgccgccg cttcagacgg
     2941 catgatagac gaagccgaac gccggactat cgaacaggaa agcggcacag accccgaaac
     3001 tgccgcatgg ctcgccgccg aataccgcct gcccgcaagc atcggggaca tcgccaccgc
     3061 cgtcggcaac gatgaggcgt tggcggcgga ggcctatctg gcggcaaggt tggtctgtgc
     3121 cgatttgtcg cggaaagaaa ccgtcttcct cgcccgcctg tcgcaggctt tgaaactgga
     3181 tgacaatctg gtggagagtt tggaaaggca attggggttt tgatgacaca gccagggagg
     3241 gaaaatcatc aaaaaacggt tattccgacc ccggcctcaa aacggcaa
//
LOCUS       DAWFAD010000179         3263 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWFAD010000179 DAWFAD010000000
VERSION     DAWFAD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.179"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(150..296)
                     /locus_tag="WGO49_001873"
     CDS             complement(150..296)
                     /locus_tag="WGO49_001873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306932.1"
                     /translation="MMRNGGNLSGITENERKSGQGGEAADSTDRTEPIHLVLEHLRES
                     FSLS"
     gene            1058..3199
                     /locus_tag="WGO49_001874"
     CDS             1058..3199
                     /locus_tag="WGO49_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0306933.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNAKVVLDTITVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNDNGGKT
                     VPYSALDKRSYLAKIGTSFGDDDHRIVLSHMKDQHRGIRTVREEFAVSEKNSRITIKR
                     QAPAYRETTQSNTNLAYTGKDLGFVEKLDANAYVLEKKRYSADDKDNGYAGNVKGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSQFKIEDKTGATPAEIQK
                     NRADEATAHDYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHDGKTVSSS
                     NLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTKAERARNT
                     EIGFNYNDGTFAANGSYFWQTIKDALANPQNRHDSVAVREAVNAGYIKNHGYELGASY
                     RTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFKNPNLEIGW
                     RGRYVQKATGSILAAGQKNRDKQLENVVRKGFGVNDVFANWKPLGKDTLNVNLSVNNV
                     FNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          870 a          922 c          745 g          726 t
ORIGIN      
        1 caaaagtggg aatctagaaa tgaaaagcag caggaattta tcggaaatga ccgaaactga
       61 acggactgga ttcccgcttt cgcgggaatg acggcgacag ggttgctgtt atagtggatg
      121 aacaaaaacc agtacgtcgt tgcctcgcct tagctcaaag agaacgattc tctaaggtgc
      181 tcaagcacca agtgaatcgg ttccgtccta tccgtactgt ctgcggcttc gccgccttgt
      241 cctgatttcc gttcgttttc ggttattccc gataaattac cgccgtttct catcatttct
      301 ttaacccttc gtcattcccg cgcaggcggg aatctagttt ttttgagttc cagttgtttc
      361 tgataaattc ttgcagcttt gagttcctag attcccactt ttgcgggaag gacgcggaaa
      421 ggttgctttt cgtcattccc gcgcaggctt cgtcattccc gcgcaggcgg gaatccagtc
      481 tgttcggttt cggttttttc cgatagattc ctgccgcgtt aggggttcta gattcccgct
      541 ttcgcgggaa ggacgcggaa aggttgcttt tcgtcattcc cgcgcaggcc tcgtcattcc
      601 cgcacaggct tcgtcattcc cgcgcaggcg ggaatccagt ctgttcggtt tcagtcattt
      661 ccgatagatt cctgccgcgt taggggttct agattcccgc tttcgcggga atgacgcgga
      721 aaggttgctt ttcgtcattc ccgcatttgt taatccacta tatttccgcc gttttttata
      781 gtggactaac aaaaaccagt acagcgttgc ctcgccttgc cgtactatct gtactgtctg
      841 cggcttcgtc gccttatcct gatttttgtt agtccactat acatttccga caaaacctgt
      901 caacaaaaaa caacacttcg caaataaaaa cgataatcag ctttacacaa atcccccctc
      961 tcgttaatat aaacaaaaat aattattatt tttcttatcc tgccaaacct taacggtttg
     1021 gatttacttc ccttcataca ctcaaaagga cgaacaaatg aacgccccgt ttttccgcct
     1081 cagcctgctc tcgcttaccc tggcggcagg ttttgcccat gcggcagaaa ataatgccaa
     1141 ggtcgtactg gataccatta ccgtaaaagg cgaccgccaa ggcagcaaaa tccgtaccaa
     1201 catcgttacg cttcaacaaa aagacgaaag caccgcaacc gatatgcgcg aactcttaaa
     1261 agaagagccc tccatcgatt tcggcggcgg caacggcacg tcccaattcc tgacgctgcg
     1321 cggcatgggt cagaactctg tcgacatcaa ggtggacaac gcctattccg acagccaaat
     1381 cctttaccac caaggcagat ttattgtcga tcccgctttg gttaaagtcg tttccgtaca
     1441 aaaaggcgcg ggttccgcct ctgccggtat cggcgcgacc aacggcgcga ttatcgccaa
     1501 aaccgtcgat gcccaagacc tgctcaaagg cttggataaa aactggggcg tgcgcctcaa
     1561 cagcggcttt gccagcaacg aaggcgtaag ctacggcgca agcgtattcg gaaaagaggg
     1621 caacttcgac ggtttgttct cttacaaccg caacgatgaa aaagattacg aagccggcaa
     1681 aggtttccgc aatgacaacg gcggcaaaac cgtaccgtac agcgcgctgg acaaacgcag
     1741 ctacctcgcc aaaatcggaa caagcttcgg cgacgacgac caccgcatcg tattgagcca
     1801 catgaaagac caacaccgag gcatccgcac tgtgcgtgaa gagtttgccg tcagcgaaaa
     1861 aaattcacgg ataactatta aacgccaagc ccctgcttac cgcgaaacca ctcaatccaa
     1921 caccaacttg gcgtacaccg gcaaagattt gggctttgtc gaaaaactgg atgccaacgc
     1981 ctatgtgttg gaaaagaaac gctattccgc cgatgacaaa gataacggct acgcaggcaa
     2041 tgtaaaaggc cctaaccata cccgaatcac cactcgaggt atgaatttca acttcgacag
     2101 ccgccttgcc gaacaaaccc tgttgaaata cggtatcaac taccgccatc aggaaatcaa
     2161 accgcaagcg tttttgaatt cgcaatttaa aattgaagat aaaactggtg caaccccggc
     2221 agaaatacaa aaaaaccgtg cagatgaagc cactgctcac gactacaaac tgtccaaccc
     2281 aaccaaaacc gataccggcg cgtatatcga agccattcac gagattgacg gctttaccct
     2341 gaccggcggg ctgcgttacg accgcttcaa ggtgaaaacc catgacggca aaaccgtttc
     2401 aagcagcaac cttaacccga gtttcggtgt gatttggcag ccgcacgaac actggagctt
     2461 cagcgcgagc cacaactacg ccagccgcag cccgcgcctg tatgacgcgc tgcaaaccca
     2521 cggtaaacgc ggcatcatct cgattgccga cggcacgaaa gccgaacgcg cgcgcaatac
     2581 cgaaatcggc ttcaactaca acgacggcac gtttgccgcc aacggcagct acttctggca
     2641 gaccatcaaa gacgcgcttg ccaatccgca aaaccgccac gactctgtcg ccgtccgtga
     2701 agccgtcaac gccggctaca tcaaaaacca cggttacgaa ttgggcgcgt cttaccgcac
     2761 cggcggtctg actgccaaag tcggcgtaag ccacagcaaa ccgcgctttt acgatacgca
     2821 caaagacaag ctgttgagcg cgaatcctga atttggcgca caagtcggcc gcacttggac
     2881 agcctccctt gcctaccgct tcaaaaaccc gaatctggaa atcggctggc gcggccgtta
     2941 tgttcaaaaa gctacgggtt cgatattggc agcaggtcaa aaaaaccgcg acaaacaatt
     3001 ggaaaacgtt gtacgcaaag gtttcggtgt gaacgatgtc ttcgccaact ggaaaccgct
     3061 gggcaaagac acgctcaatg tcaatctttc agtcaacaac gtgtttaaca cgttctacta
     3121 tccgcacagc caacgctgga ccaataccct gccgggcgtg ggacgtgatg tacgcttggg
     3181 cgtgaactac aagttctaaa acgcacatcc cgaaaaaatg ccgtctgaaa gcctttcaga
     3241 cggcatctgt tctgataatt tga
//
LOCUS       DAWFAD010000180         3206 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWFAD010000180 DAWFAD010000000
VERSION     DAWFAD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.180"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(93..1193)
                     /locus_tag="WGO49_001875"
     CDS             complement(93..1193)
                     /locus_tag="WGO49_001875"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ0306934.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGADELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAERTRIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDNRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADTRQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            1518..3092
                     /locus_tag="WGO49_001876"
     CDS             1518..3092
                     /locus_tag="WGO49_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ0306935.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
BASE COUNT          662 a          802 c          941 g          801 t
ORIGIN      
        1 gtttaagtgt aaatggattt taagggtttg taaacaaaag gggcgaaaat gccgtctgag
       61 cggcggaaat ggctttcaga cggtatttgc gctcaataat aatatcccgc gcccagaata
      121 cacggtttgg atgcgccggt tgctttgtgc gggctgccgg gaatgcggtt aatccaacac
      181 gccgccaacc acgcaaatgc ggctgcctcc acccattgag gatcgaggtt cagttcggcg
      241 gtgctgtgca gggaaacgcg tgtgccgaaa cattctgcca aatccgccat taaaacagga
      301 ttgcggatgc cgccgccgca aatatacatt tgacgggtat ctgccgctgc gtgtgagacg
      361 gcgtcgaaaa cggtttgcgc ggtaaaacgg gaaagcgtcc gcaatacgtc gtatcggttt
      421 tcgccgccgt caaggtaggt ttcgagccaa tttagggcaa acagttcgcg ccccgtgctt
      481 ttggggtggg gtcgtgcgaa atacgggtgg gcgagcagcc tgtcgagcag ttgcggcaat
      541 atgttgcctt gtgccgcctt tgcaccgttt ttgtcgtaag gaagctgcca gtgtgcctgc
      601 gtccacgcgt ccatcagcat attgcccggc cctgtgtcga agccgaaggc gggtgcgtcg
      661 ggggggagta cgctgatgtt ggcaatcccg ccgatgttca gtaccgcgcg tgtttccctg
      721 ttgtcgcgga acagggcttc gtgaaaggcg gggacgagtg gcgcgccttg tccgccggcc
      781 gcaaggtcgc ggctgcggaa gtcgccgacg gtaaaaatcc gcgtccgttc cgccagcagc
      841 ggcaaatcgg caagctgtat gctgtaaccg tgttccggcg cgtgtcggac ggtttgcccg
      901 tggcagccga gggcggtaat gtcggatggt gcgaggtttt gactgcacag cagttcggcg
      961 gcggtttgcg cgtacaggcg gctgagttct tgcgacaaaa tcctgctgcg gtgcagttcg
     1021 tctgcgcctg tgtcctgcaa atccagcaat tggcggcgca gcctgtcggg atagggggta
     1081 aaggcgtgcc cttccgcgcc cagccatttg ccgccgtcca tccgtatcag tacggcatcc
     1141 gccccgtcca tgctggttcc cgacatgatg ccgatgtaaa gctgtgtttc catcatcact
     1201 cccaaactgg tgcaaaacgc cattttaacg tgtattgacg ctcgtatacc gatttgccgc
     1261 cgcagtgtaa ataaagtgta aataaatgtt tcaagacgga tggaaaaata ttataatgcg
     1321 cccgcaacat ccagtagtag aagtgtcata caaaccgttt ccggcagcag ttttgcattc
     1381 ggtcaggttt gggggtattt ggatgcggtt cagaaggaag gatgcgtctg ccatatcccg
     1441 aaacggcagt tcgaccggag gcagcagtac agtgtcggca acactcatga tttccaccac
     1501 attaaaggaa gattgccatg gctcaaatcc aaatgagcgc aaatgttaaa accatcaacg
     1561 ccgtctttgc cgccatgctg gtaggtacag tcggctattt tatttattgg ggcttgggtt
     1621 atacccatta caattacgcc gccttattca ttattgccac gatgttcggc gtgtttatgg
     1681 cgttcaacat cggcggcaac gatgttgcca attctttcgg caccagcgtc ggtgcgggta
     1741 cgctgaccat cccgcaggct ttgctgattg cggcggtatt tgaggtcagc ggcgcggtca
     1801 tcgcgggcgg cgaggtaacc aataccatac gcaaaggcat cgtcgatttg aagggtgttg
     1861 atttcgaacc catacagttt gtgtttatta tgatgtccgc gcttttggcg gcggcgttgt
     1921 ggctgttgtt tgcctcgaaa aaagggcttc cggtatctac cacccattcc attatcggcg
     1981 gcattgtcgg cagcgcggta tgtatggcgg taatgaacga tgccgcatcg ggcgatttga
     2041 tacgttgggg caagctgggc ggtattggtg tttcttgggt attgtcgccc gtgttgggcg
     2101 gcgcggtgtc ctatttcctg ttttcgcgcg tcaagaaaaa cgtcttagat tacaacgctt
     2161 gggcggaagg cacgctcaag ggcatcaagc aggaaaaaaa ggcctataaa gaacggcacc
     2221 gcctgttttt cgagggtttg tccgaagccg aaaaagtcga gtacgccacc aaaatggcgc
     2281 acgacgcgca aatttacgac gaacccgaat tcgatccgca agagctgcaa tcggagtatt
     2341 accgcggtct ttatgcgttc gacaaccgta aaaacaatgt cgattcctac aaagcactgc
     2401 attcttggat tccctttatc gcttcgttcg gcgcgatgat gatttccgct atgctgattt
     2461 tcaagggctt gaaaaacctg catttgggga tgagcaacgt caacagcttc ctgaccatct
     2521 ttatgatagg cgcggcggtg tggatgggga cgtttgtgtt tgccaaaagc ctcaagcgta
     2581 aagacttggg caaatcgacc tttcagatgt tttcatggat gcaggtcttt accgcctgcg
     2641 gcttcgcatt cagccacggg gcgaacgata tcgccaacgc catcggtccg tttgccgcga
     2701 ttatggatgt tttgcgtacc aacagcgttg ccgcgcaaaa tgtcgtcccc ccgattgcga
     2761 tgctgacttt cggcatcgcg ctgattgtcg gtttgtggtt tgtcggtaaa gaggtgatta
     2821 aaaccgtcgg tacgagtttg gcggaaatgc accccgcctc gggttttacc gccgaactgt
     2881 ccgccgcatc cgtcgtgatg ggcgcgtcgc tgatggggct gcccgtgtcc agtacgcata
     2941 tcttggtcgg cgcggtactc ggcatcggtc tggtcaaccg caatgccaac tggaaactga
     3001 tgaagcccat cggtttggcg tgggtcatta ccctgcctgc cgccgccgta ttgtcggttg
     3061 tctgctactt ggttttacag gcagtattct gattgtaaaa tactgatgcc gtctgaaccc
     3121 gtgttcagac ggcattttgt tgatggaatg tgcgggcttg tgccttatgc acaatctgtt
     3181 ctgtcgggat atgccgtttg gtatag
//
LOCUS       DAWFAD010000181         3158 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWFAD010000181 DAWFAD010000000
VERSION     DAWFAD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.181"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(127..999)
                     /gene="hpnD"
                     /locus_tag="WGO49_001877"
     CDS             complement(127..999)
                     /gene="hpnD"
                     /locus_tag="WGO49_001877"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ0306936.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLLRVRYGSFEELKLYCRRVAGVVGCLIARILGFSDDQTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMRRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPDADKKAQKVGLVMAAVYYALLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            1170..3032
                     /gene="hscA"
                     /locus_tag="WGO49_001878"
     CDS             1170..3032
                     /gene="hscA"
                     /locus_tag="WGO49_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ0306937.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLVGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDGAITRMLKDSMDNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAKELQQI
                     RQGIADLQGRLKDGKAEDIRSAVAKLSRSTDNFAAKRMNRNIQRALTGQSVDNI"
BASE COUNT          732 a          946 c          837 g          643 t
ORIGIN      
        1 atttcaaagc gcgaacgcca acgggattgc gcggttacga tgcagacttt cagacgatgg
       61 ttgaaacccc gtccggcggg gctgtgggaa tcgggtttgc ctgacgggcg gcgggcggta
      121 tgcgccttat gcccgttccg gcgtgccggg gcgcggtttg aatccgaata accaggtttt
      181 cagggcaatg cgttttttgc gcggcgaagg gagggcgatt ttgtatttga ggacgttttg
      241 cgcgccgtct cggtcgattt cgtttaaaag cgcgtaataa accgccgcca tgaccagtcc
      301 gactttttgg gcttttttat cggcatcagg cagcagcgat acggcttcgc ggtaggtttc
      361 gcgggcgcgt ttgatttgga acgccatcaa ttcggcaaaa ttgcccgtcg ggctgcattg
      421 caaaatcacg cttgcgggga catcaaaccg ccgcatttcc tccatcggca ggtaaatccg
      481 tcccctgcgc gcgtcttcgc cgacatcgcg gatgatgttg gtcagttgca gcgcaagtcc
      541 catcttgtcg gcgtattcca gcgtttggtc gtctgaaaac cccaaaatcc gcgcaatcag
      601 gcagccgacc acgcccgcga cgcggcggca atacagtttt aattcttcaa aactgccgta
      661 acgcacccga agcaaatcca tctgcatccc gtcgattaag gcttccagtt catatttcgg
      721 cagcttgaag gtttccttaa cttgccgcaa ggcctgattg acggggtgtt ccggcatcgc
      781 gccgccgaat accttgtcca aatcgccgcg ccaccagttc aatgtcgcct gtgcaacatc
      841 ggggttggaa cattcgtcaa ccacatcgtc caattcgcgg caaaaagcat ataaaaccgt
      901 taccgcatcc cgtttttcct gagtcaggaa acggaagccc gacaaaaaac tggagcggct
      961 ttcttctgct ttttggcggc aatagtcgag tcctttcacg atttatattc ctaatgatgg
     1021 gcgggaaagg cggattttat cggcatttgg cggtagaggg caatttcggc ggcacgaccc
     1081 aatccttagc ggtttgctca actatcggcg caaattctgt taaaatgccg ctttccttcc
     1141 tttacacacc gcaccgacag gcagaattta tggctctttt gcagatttca gaaccgggta
     1201 tgtccgccgc cccgcaccgg caccgtttgg cggcaggcat cgatttgggt acgaccaaca
     1261 gcttggtcgc caccgtccgc agcggcagtg ctgcctgtct gcccgatgcc gaagggcgcg
     1321 ttaccctgcc ttccgtcgtc cgctatctgg aaaacggcgg cattgaagtc ggcaaaaccg
     1381 ccctgtccgc ccaaaaaacc gacccgctga acaccgtcag ctccgccaaa cgccttatcg
     1441 ggcggacgct tgccgatctg catcaaaata cgcattacct gccttaccgt ttcggcgaca
     1501 atcaacgcgt tatcgaactg catacgcggc agggggtgaa aacgcctgtc gaagtgtcgg
     1561 cggaaatcct caaaaccctt aaatcgcgcg ccgaagaaac cttggtcggc gatttggtcg
     1621 gcgtggtgat taccgtccct gcctatttcg atgatgccca acgccaagcc accaaagatg
     1681 ccgcgcgtct ggcgggtttg aacgtattgc gcctgctcaa cgaacccacc gctgccgcaa
     1741 tcgcctacgg gctggacaac gcctcggaag gcacgtttgt cgtgtacgac ttagggggcg
     1801 gcacattcga cgtatccgta ttgcaactga ccaaaggact gtttgaagtc aaagccaccg
     1861 gcggcaacag cgcgttgggc ggcgacgatt tcgaccaccg cctgttctgc cgcctgctcg
     1921 aacaaaacgg actctcccaa ctcaacgaac aagacagcca actcctgctc tcgctcgtcc
     1981 gcgccgccaa agaacaatta accacgcaaa ccgaagcgcg cattcaggcg acgctttcag
     2041 acggcatggc aatcgacaca agcatcagtc gcgccgagtt ccacaacctg acgcagcatt
     2101 tggtgatgaa aacgctcgaa ccggtcacac aggcgttgaa agatgccggt gtcggtaaaa
     2161 acgaagtcaa aggcgtgatt atggtcggcg gttcgacccg tatgctgcac gtccaacagg
     2221 cagtcgccac cttcttcgga caaaccccgc tgaacaacct caatcccgac gaagtcgtcg
     2281 cgctcggcgc cgccatacag gcaaacgtcc tcgcaggcaa caaaaccgac ggcgaatggc
     2341 tgctgctgga cgttacacct ttgtcgctcg gtttggaaac ctacggcggc ttggcggaaa
     2401 aaatcatccc gcgcaattcc accatcccca ccgcgcgcgc gcaggacttt accaccttca
     2461 aagacggtca aaccgcgatg acgatacacg tcgtgcaagg cgagcgcgag ctcgtttctg
     2521 actgccgcag ccttgccaaa ttcaccctgc gcggcattcc accgatggcg gcgggtgcgg
     2581 cgcgtatccg cgtaaccttc caaatcgatg cggacgggct gctgtccgtt tccgcccaag
     2641 aacaaagcac cggcgtacag gcgcaaatcg aagtcaaacc ttcctacggc ttggacgacg
     2701 gcgcgattac ccgaatgctt aaagacagta tggacaacgc cgccgaagat atggcggcac
     2761 gcgcccgcgc cgaagccgta gtcgaagccg aaagcctgac cgatgccgtc aacgccgccc
     2821 tcgagttgga cagcgattta ctggatgcca aagaattgca acagatccgg caaggcatcg
     2881 ccgatttgca aggccgtctg aaagacggaa aagccgaaga catccgctcc gccgtcgcca
     2941 aactcagccg cagcaccgac aacttcgccg ccaaacgcat gaaccgcaat atccaacgcg
     3001 cattgacagg ccagagtgtc gataatattt gatacttaaa cggtttcaga cggcatagat
     3061 ataacctgat gccgtctgaa ggctcgaaaa cacttgaaaa acatcgatat ggaaaagtca
     3121 ggcattgtct attcgatgaa aaccgtcatc aagggcgt
//
LOCUS       DAWFAD010000182         3158 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWFAD010000182 DAWFAD010000000
VERSION     DAWFAD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.182"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            28..2997
                     /locus_tag="WGO49_001879"
     CDS             28..2997
                     /locus_tag="WGO49_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0306938.1"
                     /translation="MNEGNQPEVKQNGQYSTIKDKDREREYTYHQGTRGGGSVSFNNS
                     DELVSRQSGTAVFGTATYLPPYGKVSGFDEKRLTERGNALNWINTTHPGLIGYSYAGV
                     VCRDSTSCPKLVYKTRFSFDNTGLAKNAGSLDRHPDPSRENSPIYKLKDHPWLGVSFN
                     LGSENTVKNGNSFNKLISSFSEDNNNQTIVSTTEGSPISLGDQQREHTAVVYYLNAKL
                     HLLDKKQIQNITDKTVQLGTLRPRVETTGRSWLNFWATWKIEDKGNITVRLDLPEVKA
                     GRCVNANNPNKSTKAPSPALTAPALWFGPVQNGKAEMYSASVSTYPDSSSSRIFLQNL
                     KRKNDSNRPGRHSLETLTENDIKSRQPNFTGRQTIIRLDSGVQQIKLQGNEVANFNRN
                     DGKNDTFGIVSEYGLTPDANEWKKVLLPWTVRAFNDDGRFNTVNKEENNGKPKYSQRY
                     RIRENGNNGNNGKRDLGDIVNSPIVAVGEYLATSANDGMVHIFKQSGGDKRSYNLKLS
                     YIPGTMPRKDIQNTESTLAKELRAFAEKSYVGDRYGVDGGFVLRQVELSGKKHVFMFG
                     AMGFGGRGAYALDLTKAENGNPTAVSLFDVKHDNNGKNSNNSVQLGYTVGTPQIGKTH
                     NGKYAAFLASGYATKTIDSTDNKTALYVYDLESSGTLIKKIDVPGGKGGLSSPTLVDK
                     DLDGIVDIAYAGDRGGNMYRFDLSGNNPNSWTVRPIFEGTKPITSAPAISQLKDKRVV
                     IFGTGSDLSEDDVDNKDIQHVYGIFDNDTDTGTAQDGQGKGLLEQVLSEENKTLFLTD
                     YKRSNGSGSKGWMVKLQPRQRVTVKPTVVLRTAFVTIRKYKDNGCGAETAILGINTAD
                     GGKLTKKSARPIVPAANQAVAQYSGHKQTTKGKSIPIGCMQKGNEIVCPNGYVYDKPV
                     NVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMNDLDSLDI
                     TGPTCGMKRISWREVFY"
BASE COUNT          911 a          819 c          794 g          634 t
ORIGIN      
        1 gcgcaaaccc ataaatacgc tattatcatg aacgagggaa accagcccga ggtaaagcag
       61 aatgggcaat attcaacaat aaaggacaaa gacagggagc gcgaatatac ttatcatcag
      121 ggcacaagag gaggaggctc tgtctcattc aacaatagcg atgagcttgt ttctcgacaa
      181 agcggtactg ccgtttttgg cacagccacc tacctgccgc cctacggcaa ggtttccggt
      241 tttgatgaaa agaggctgac agagcgcggc aatgccctta attggattaa tacgacccac
      301 ccagggttga taggctacag ctacgccggt gtcgtatgca gagacagcac aagctgtccc
      361 aaacttgtct ataaaacccg attttccttc gacaacaccg gtttggcaaa aaatgcgggc
      421 agcctggata ggcacccgga cccaagccgc gaaaattcgc ccatttacaa attgaaggat
      481 catccatggt tgggcgtgtc tttcaatttg ggcagcgaga ataccgtcaa aaatggcaac
      541 tcattcaaca aattgatatc ttcttttagt gaagacaata ataatcaaac catcgtctct
      601 acgacagaag gctcccctat ttcccttggc gaccagcagc gcgaacatac cgccgtggtc
      661 tattatctga acgccaagct gcacctgctg gataaaaaac agattcaaaa tatcaccgac
      721 aaaacagtgc agttgggcac cttgagaccg cgcgtcgaga cgacaggacg aagctggcta
      781 aatttttggg ctacgtggaa gattgaagat aaagggaaca ttacagtccg cctcgacctg
      841 ccggaagtca aagcaggccg ctgcgtcaac gcaaataacc ccaataagag taccaaagcc
      901 ccttcccccg cactgaccgc ccccgcgctg tggttcggcc ctgtgcaaaa tggtaaggcg
      961 gagatgtatt ccgcttcggt ttccacctac cccgacagtt cgagcagccg catcttcctt
     1021 caaaatctga aaagaaaaaa cgactccaac agacccggcc gccattccct cgaaaccttg
     1081 actgagaacg atattaaaag tcgacagccg aatttcacag ggcggcaaac aatcatccga
     1141 ttggatagcg gcgtacagca gatcaaactt caaggcaatg aggtcgccaa ttttaataga
     1201 aatgacggca aaaacgacac tttcggcatt gttagtgaat atggcctcac gcctgatgcc
     1261 aatgagtgga aaaaagtgct gctgccttgg acggttcgtg ctttcaatga tgacggtcga
     1321 tttaacacag tcaacaaaga agaaaacaac ggcaagccaa aatacagcca aagataccgc
     1381 atccgcgaaa acggcaacaa cggcaacaac ggcaagcgtg atttgggcga catcgtcaac
     1441 agccccatcg tggcggtcgg cgagtatttg gctacttccg ccaacgacgg gatggtgcat
     1501 atcttcaaac aaagcggcgg ggacaagcgc agctacaatc tgaagctcag ttatatcccg
     1561 ggtacgatgc cgcgcaagga tattcaaaac accgaatcca cccttgccaa agagctgcgc
     1621 gcctttgccg aaaaaagcta tgtgggcgac cgctacggcg tggacggcgg ctttgttttg
     1681 cgccaagtcg aactgagcgg gaaaaaacac gtgtttatgt tcggcgcgat gggttttggc
     1741 ggcagaggcg catacgcctt ggatttgacc aaagccgaaa acggtaaccc gaccgccgtt
     1801 tccctgtttg atgtcaaaca tgacaataat ggcaagaata gcaataatag cgtgcaatta
     1861 ggctacaccg tcggcacgcc gcaaatcggc aaaacccaca acggcaaata cgccgccttc
     1921 ctcgcctccg gttatgcgac taaaaccatt gacagcacag acaataaaac cgcgctgtat
     1981 gtgtatgatt tggaaagcag cggtacgctg attaaaaaaa tcgatgtacc cggcggcaag
     2041 ggcgggcttt cgtcccccac gctggtggat aaagatttgg acggcattgt tgatatcgcc
     2101 tatgccggcg atcgcggcgg gaatatgtac cgctttgatt taagcggcaa caacccgaac
     2161 agttggactg tacgccctat ttttgaaggc acaaaaccga ttacttccgc gcccgccatt
     2221 tcccaactga aagacaaacg cgtggtcatc ttcggcacgg gcagtgattt gagtgaggat
     2281 gatgtggaca acaaagacat ccaacacgtt tacggtattt ttgacaatga cacagacacg
     2341 ggtacggcgc aagacgggca gggcaaaggg ctgctcgagc aagtgcttag tgaggaaaat
     2401 aaaaccttat tcctgaccga ttacaagcga tccaacggat cgggcagtaa ggggtggatg
     2461 gtgaaattgc agcccagaca gcgcgttacc gtcaaaccga ccgtggtatt gcgtaccgcc
     2521 tttgtaacca tccgcaaata taaagacaac ggctgcggcg cggaaaccgc cattttgggc
     2581 atcaataccg ccgacggcgg caagctgacc aagaaaagcg cgcgcccgat tgtgccggca
     2641 gccaatcagg ctgtcgcgca atattccggc cataagcaaa ccaccaaagg caaatccatc
     2701 cctataggtt gtatgcaaaa aggcaatgaa atcgtctgcc cgaacggata tgtttacgac
     2761 aaaccggtta atgtgcgcta cctggacgaa aagaaaacag acggattttc aacaacggca
     2821 gacggcgatg cgggcggcag cggtatagac cccgccggca agcgttccgg caaaaacaac
     2881 cgctgcttct cccaaaaagg ggtgcgcacc ctgctgatga acgatttgga cagcttggac
     2941 attaccggcc cgacgtgcgg tatgaaacga atcagctggc gtgaagtctt ctactgattt
     3001 gcacgcgaaa atgccgtccg aaaggttttc ggacggcatt ttttgcgttt ttcgggaggg
     3061 gcgggttcgt aaaaggcggg ctatagggta ggcttcatct cgccaatctc actgaatcca
     3121 tcaatttcca caattcaatt aaataccgtc aaaccgat
//
LOCUS       DAWFAD010000183         3139 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWFAD010000183 DAWFAD010000000
VERSION     DAWFAD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.183"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(550..1134)
                     /gene="rnhB"
                     /locus_tag="WGO49_001880"
     CDS             complement(550..1134)
                     /gene="rnhB"
                     /locus_tag="WGO49_001880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ0306939.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAAGPEEIASLNILHATMLAMKRAVDGLAVRPEKIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALEKYGVLPEHRRDFAPVRNLFAQQALF"
     gene            complement(1202..3097)
                     /gene="mnmG"
                     /locus_tag="WGO49_001881"
     CDS             complement(1202..3097)
                     /gene="mnmG"
                     /locus_tag="WGO49_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ0306940.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKATIREMLENQENLDLFQQAVEDVTLDGERISGVVTAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGSADMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIQGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPDGIDYGKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
BASE COUNT          550 a          760 c          979 g          850 t
ORIGIN      
        1 tcattcccgt aaaaacaaaa aatcaaaaca gaaaactgaa atatcgtcat tcccgcgcgg
       61 gcgggaatct aggtctgtcg gtacggaaac ttatcgggaa aaacggtttt tccaaccctg
      121 agactccgga ttcctgtttt cgcgggaatc cggttttttg agtttcagtc atttttgata
      181 aattcttgta gctttgagtt tctagattcc cgcttttgcg ggaatgacgg tttggaagtt
      241 acctgaaatt caaaaaaaaa cggaaaccgg acggattgga ttcccgtttt cacgggaatg
      301 acggatttta ggtttctttt ttgattttct gtttttcgcg ggaatggcgg tttggaagtt
      361 acctgaaatt caaaaaaaaa aaacggaaac cggacggatt ggattcccgc ctgcgcggga
      421 atgacggatt ttaggttttt ttttttgatt ttctattttt cgcaggaatg acggtttggg
      481 ttctttctct ttggagttgc gatgccggaa atgccgtctg aaggcttcag acggcatttt
      541 tgtgccggtt taaaacaagg cctgctgcgc gaacaggttt ctgacggggg cgaagtcgcg
      601 gcggtgttcg ggcagcacgc cgtatttttc gagggcttcc aaatgctgct tcgtgccgta
      661 acctttgtgt ttgtcgaaac cgtattgggg atggcgttgc gccagtgcgt acatttccgc
      721 atcgcgggcg gtctttgcca aaacggatgc ggcggatatt tcgatgattt tgctgtcgcc
      781 tttgacgacg gcttcggcag ggatgttcaa atgttcggga atgcggttgc cgtcgatgaa
      841 tattttttcg ggacgcacag ccaaaccgtc aacggcgcgt ttcatcgcga gcatggtggc
      901 gtgcaggatg ttgaggctgg cgatttcttc gggcccggcg gcggcgacgt gccacgcaac
      961 cgcctgattt tttatcattt cggcaagcgc gtcgcgtttt ttctcgctga gttttttgga
     1021 gtcggtcagt ccgggcaggt cgaatatttc cggaaggatg acggcggcgg caaacacgct
     1081 gccgactaaa ggcccgcgcc ctgcctcgtc cacgccggcg gtcagtatgt gcatgatgtt
     1141 tcctgtcggg atggcacgat gccgtctgaa cggtgtttca gacggcatcg cgccgatgtg
     1201 tttatttcgc gtctttaaac ccgcgcttca aatgcaccat cagcaatgcc actgccgcag
     1261 gcgttacgcc ggaaatgcgg cttgcctgcc cgacggtttc aggtttgtgc tggttgagct
     1321 tttgctgcac ttctgccgac aagcctttga ctttgccgta atcgatgccg tcgggcagtt
     1381 ttaaggtttc gatgtcgcgg cggctgtcga tttcttcgtt ttggcggtcg atatagcctt
     1441 ggtatttgac ttggatttcg acttgttcga ccacttcggc ggagaggcgt tcagacggca
     1501 tcgcgccttc gagcgtcatc agcgcggcgt agtcgaggtt cgggcggcgc aggaggtcgt
     1561 gcaggttggc ttcgcggctg agcttttgcc cgaatacgcg gatttgttcg tcttcggcga
     1621 gtttttgcgg cgtgtaccac gttgttttca aacgttggat ttcgcgttcg atggcttcgc
     1681 gtttttcgtt gaacatgcgc cattgcgctt cacccaccaa gccgattttg tagccgtctt
     1741 cggtcaggcg catatcggcg ttgtcttccc tgagttgcag gcggtattcg gcacggctgg
     1801 tgaacattcg gtagggttcg ttcacgcctt tggtgatgag gtcgtccacc aatacgccga
     1861 gataggcttg ttcgcggcgc agcaggagcg ggtcttgttc gcggacgtat tgcacggcgt
     1921 tcgcgcctgc cagcagtcct tgcgcggcgg cttcttcgta gcccgttgtg ccgttgattt
     1981 gtccggcgaa gaagaggcct tggatggttt tggtttcgag gctggctttg aggttgcgcg
     2041 ggtcgaagta gtcgtattcg atggcgtagc cagggcgcag gatatgggcg ttttcaaggc
     2101 ctttcatgct gcgcacgagc gcgatttgga tgtcgaacgg caggctagtg gagataccgt
     2161 ttggatagta ttcgtgcgtg gtcaggcctt cgggctcgag gaaaatctgg tggctgtctt
     2221 tgtcggcgaa gcggttgatt ttgtcttcga tagacggaca ataacgcgga cccacacctt
     2281 cgattttgcc agtaaacatg gggctgcggt cgaagcctga acggatgatg tcgtgggttt
     2341 gcgtgttggt atgcgtaatc cagcaggaca cttggcgagg gtgcatatcg gcgctgccgc
     2401 gtacggacat tacgggaacg ggcgtgtcgc cgggctgttc ggtcagttgg gagaagtcaa
     2461 tcgtgcgccc gtcaatacgc ggcggcgtgc cggttttcag acggccttgc ggcaggttca
     2521 attcgcgcaa acggccgccc agcgatttgg cggcggggtc gccagcgcgg ccgccttcgt
     2581 agttttccaa accgatgtgg attttgccgg acaaaaacgt gccagcagtt agcacgacgg
     2641 cgcgtgcttt aaactccacg cccatcgcgg taaccacgcc gctgatgcgt tcgccgtcga
     2701 gcgttacgtc ttcgacggct tgttggaaaa ggtcgaggtt ttcttggttt tccaacattt
     2761 cgcggatggt ggctttgtac aggatacggt cggcctgcgc gcgcgtggct cgcactgccg
     2821 cgcctttgct ggcgtttagg cggcggaact ggatgcctga tttgtcggtg gccaacgcca
     2881 tcgcgccgcc gagcgcgtcg agttcgcgta ccaaatgccc tttgccgatg ccgccgatag
     2941 aggggttgca cgacatttgt ccgagcgttt cgatattgtg tgtgagcaaa agcgtctgcg
     3001 cgcccatacg ggcggcggcg agcgcggctt ccgtgcctgc gtgtccgccg ccgacgacga
     3061 taacgtcgta ggttttgggg taaatcatgt gggtcatgtg tgtattgcct gacggtgttt
     3121 cagacggcat ttatagtgg
//
LOCUS       DAWFAD010000184         3101 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWFAD010000184 DAWFAD010000000
VERSION     DAWFAD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.184"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..211)
                     /locus_tag="WGO49_001882"
     CDS             complement(<1..211)
                     /locus_tag="WGO49_001882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306941.1"
                     /translation="MLPTRKTARKIQARHIALPFSGKPQFYRPQERLSKQAQATPPGH
                     FRFHRLSVVRIMQQHHQRITLFGRQQ"
     gene            271..894
                     /locus_tag="WGO49_001883"
     CDS             271..894
                     /locus_tag="WGO49_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0306942.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1005..2387
                     /locus_tag="WGO49_001884"
     CDS             1005..2387
                     /locus_tag="WGO49_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0306943.1"
                     /translation="MARDNRIQMFPHEWRTSTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLIAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2391..2915
                     /locus_tag="WGO49_001885"
     CDS             2391..2915
                     /locus_tag="WGO49_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ0306944.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          623 a          839 c          937 g          702 t
ORIGIN      
        1 cctgctgccg cccgaaaagc gtgatgcgct gatggtgctg ctgcataatc cggacgacgg
       61 ataaccggtg aaaacggaaa tgcccgggcg gtgttgcctg tgcctgtttg gacaagcgtt
      121 cttgagggcg gtagaattga ggtttgcccg aaaagggcag ggcgatatgc cgcgcttgga
      181 ttttgcgcgc ggtttttcgc gttggaagca atcattgtct gtttgaatat ttaatgccgt
      241 ctgaaacgtc cggacgcgcc cccgttccct atgaaaaaac cgaccgatac cctacccgtt
      301 aatctgcaac gccgccgcct gttgtgtgcc gccggtgcgt tgttgctcag tcctctggcg
      361 cacgccggcg cgcaacgtga ggaaacgctt gccgacgatg tggcttccgt gatgaggagt
      421 tctgtcggca gcgtcaatcc gccgaggctg gtgtttgaca atccgaaaga gggcgaacgt
      481 tggttgtctg ccatgtcggc acgtttggca aggttcgtcc ccgaggagga ggagcggcgc
      541 aggctgctgg tcaatatcca gtacgaaagc agccgggccg gtttggatac gcagattgtg
      601 ttggggctga ttgaggtgga aagcgcgttc cgccagtatg caatcagcgg tgtcggcgcg
      661 cgcggcctga tgcaggttat gccgttttgg aaaaactaca tcggcaaacc ggcgcacaac
      721 ctgttcgaca tccgcaccaa cctgcgttac ggctgtacca tcctgcgcca ttaccggaat
      781 cttgaaaaag gcaacatcgt ccgcgcgctt gcccgcttta acggcagctt gggcagcaat
      841 aaatatccga acgccgtttt gggcgcgtgg cgcaaccgct ggcagtggcg ttgattttga
      901 acccgcgccg caaccgaaat acggcgaatc ctgtataatc cgaaaatctg ttcactggaa
      961 gttcagacgg cattgcaact gttgatgccg tctgaaaaaa tatgatggca agagacaacc
     1021 gcatccaaat gtttccgcac gaatggcgca ccagtacgac gctttccggc gtgtacgcgc
     1081 tgcgtatgct gggtatgttc ctcgtgctgc ccgtattggc ggtgtatgcc gcctcgctgc
     1141 ccggcgcgga aggcaacaaa acgctggtcg ggctggcaat gggcatttac gggctgacac
     1201 aggctctgct gcaactgcct ttgggcatcg cttccgacaa gttcgggcgc aagaaaacca
     1261 tttatgtcgg gctgattgtg tttgcagcgg gcagcttcct tgccgccgcc gccgatacgc
     1321 tgcccatgct gatcgccgca cgcgccatac agggtgcggg agcggttagt gcggcggtta
     1381 ccgcgctgct ggcggatttg acgcgcgacg gcgtacgtac ccgcgcaatg gcgatgatcg
     1441 gtttgagtat cggtctgacg ttttcggtca gcctcgtcgt tgcccctgtg attgccgacg
     1501 cggtgggcgt acgcggcttg tttctgctga ccggtatttt gaccgttgcc agtatcggcg
     1561 tggtggcgtg gatgactccc gacccggagg tttccaagct gcacgaagat acgcaggcgc
     1621 agccttcgcg cataggcgaa gttttgaaaa accgtaggct gctgacgctc gatttcggca
     1681 ttttcgccct gcacgccgcg caaatggcat tgtttaccgc gctgcctttc gcgatgaccc
     1741 agctcggttt ggaaaaaata cagcattgga aagtctatct gccctcgacc attacgggct
     1801 tggtggtgat ggttccgctg attatcgtcg gcgagacgcg caacaagctc aaacaggttt
     1861 ttgttttggg tatcgtctgt attgcggcgg cgcagctcgg tttgctgtcc ggtatgcgct
     1921 cggtaggctt gattaccgct tatttggttg tttactttat cggttttaat gtgttggaag
     1981 cgagcctgcc gtctatggtt tccaaaatcg cgccgtccga cctgaagggt acggcgatgg
     2041 gcgtgtacaa cacgatgcag tcgctcggac tgtttgccgg cggcgcggca ggcggtttgc
     2101 tgtttcaaaa atacggcttt tccggcgtgt ttgccttttg cagtatattg atgctgctgt
     2161 ggctggtaat tgccgtttta tcgcctgcgc ccaagcccgt caaaaacctc agttaccctg
     2221 tcggcggcgt gtggcagggc aatcaggaag ggttataccg cgccttgtcg gagcttgagg
     2281 gtgtggaaga catcggtttc agtttcgacg ggcagaccgt ctatctcaaa gtgttgcaga
     2341 agggtttcga tcaggctgcc gctgaaaaaa tcatcacagg agtttaaaaa atgtcattga
     2401 acaaagtcat cctcatcggc cgcctcggac gcgatcccga agtgcgctat atgcccaacg
     2461 gcgaggcggt ttgtaatttc agcgtcgcca ccagcgaaac ttggaacgac cgcaacggcc
     2521 aacgtgtaga gcgtactgag tggcacaaca tcaccatgta ccgcaaactg gcggaaattg
     2581 ccgggcaata cctcaaaaaa ggcgggctgg tttatctgga aggcagaatc caaagccgca
     2641 aataccaagg caaagacggc atcgaacgca ccgcttacga tattgtcgcc aacgaaatga
     2701 aaatgttggg cgggcgcaat gaaaacagcg gcggtgcgcc ttacgaggaa ggttacggtc
     2761 agagtcagga ggcttaccaa cgccccgcgc agcaaagccg gcagcccgcc tccgacgcgc
     2821 cgtcccatcc ccaagaagca ccagccgcgc cgcgccgcca acccgtgcct gccgccgccc
     2881 cggtcgagga cattgacgac gatatcccgt tctgaatttt acggccggac atcctcgcgg
     2941 agggaaatca taaaggacgg agaaacctta aaccttacgg ggcagggttt ctcttttttg
     3001 catttgggcc ggcttgttgc cgccctttgc gttgggcgga acgttgcgat tgcgccgttc
     3061 gggcgttttg aggcagtgat gcctgccgca cgtccccgct g
//
LOCUS       DAWFAD010000185         3097 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWFAD010000185 DAWFAD010000000
VERSION     DAWFAD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.185"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(311..787)
                     /gene="greA"
                     /locus_tag="WGO49_001886"
     CDS             complement(311..787)
                     /gene="greA"
                     /locus_tag="WGO49_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ0306945.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1182..2483
                     /gene="aroA"
                     /locus_tag="WGO49_001887"
     CDS             1182..2483
                     /gene="aroA"
                     /locus_tag="WGO49_001887"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ0306946.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVQIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGKRQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVAN
                     ENYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQSFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPKTLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETTE"
     gene            complement(2526..3059)
                     /locus_tag="WGO49_001888"
     CDS             complement(2526..3059)
                     /locus_tag="WGO49_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0306947.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          648 a          830 c          841 g          778 t
ORIGIN      
        1 attttaggtt tctgattttg gttttctgtt tttgagggaa tgacgggatg caggttttct
       61 taaccctgcg tcctagattc ccgcttttgc gggaatgacg gcgacagggt tgctgttata
      121 gtggatgaac aaaaaccagt acggggttgt ctcgccttag ctcaaagaga acgattctct
      181 aaggtgctga agcaccaagt gaatcggttt cgtactatct gtactgtctg cggcttcgtc
      241 gccttgtcct gatttttgtt aatccactat aatttcctgc gtgtgtcggg tgtatcgaaa
      301 tcaagccgaa tcaaatatat cggacttcga taatgtcgta ttcgcgcacg ccgcccgggg
      361 cttggacttc cgccgtatcg ccctcttcct tgccgattaa ggcgcgggca atcggtgagc
      421 cgacatagat tttgccctgt ttgatgtcgg cttcgtcttc gccgacaatt tgataggtaa
      481 cgtgttcttc cgtttccaaa tcttccagcg taaccgtcgt accgaacacg attttgcctt
      541 cggcgtggat ttcggtcgga ttgatgatgt gggcaacgga aagtttgtgt tccagctcgg
      601 aaatgcggcc ctcgataaag ccttggcgtt ctttggcggc ttcgtattcg gcgttttcgg
      661 acaaatcgcc gtgcgaacgg gcttcggcaa tcgcttcgat cacttcggga cgcgccacgc
      721 ttttgagctg ctgcaattcc tgtttcagca attccgcacc gcgtacggtc agggggattt
      781 tttgcatcgg tcttttttct ccatattccg gcacaccgtt ttgcggcagc aagcataccg
      841 cgtaccgtct tgttttgtgc gtccggatat taaaataaaa atacaagccg cccggaaaat
      901 cggcggcttg tctgtcgttg aacagcggct attctaccaa attctatgaa attggcaatc
      961 gtgccgtgcc gccgacaaac gcgccatgtc cgcaacaaaa gctgaaaata tgccgtcaaa
     1021 gaaattttag aaacaaaaaa tttaaaaata atcaattttc ggcataaaaa accacattta
     1081 cggactttaa aaccgaaaat gccaagcctg agatttttca tacagcattt gcaccagtat
     1141 aatgcaggct gtttttatct ttataatatt gacgttttgc catgaccgaa tccgtccgcc
     1201 tccccgtcgc ccgtctcaaa ccttccaccg tcgccctgcc cggctccaaa agcatcagca
     1261 accgcaccct gctgcttgcc gccttgtccg acaatgcttg cgaaatccat tccctgctca
     1321 aatccgacga taccgaccgt atgctcgaag cactcgataa actcggcgtt caaatcgaat
     1381 atcttgccga agaccgtctg aaagtgcacg gcacaggcgg acgcttcccc aaccgcactg
     1441 ccgatttgtt tttgggcaac gcgggcacgg cgttccgtcc gctgactgcc gcacttgccg
     1501 ttttgggcgg cgattatcat ctgcacggcg tgcctcgtat gcacgaacgc cccatcggcg
     1561 atttggccga tgcgctgcgg attgccggcg ccgatgtcga atatctcggc aaggaacact
     1621 atccgccgct tcatatcggc aaacgtcaag acaacggcga acgcgtgatt ccgattaaag
     1681 gcaatgtgtc cagccagttt ctgaccgccc ttttaatggc gttgccgctg accgggcagg
     1741 cgtttgaaat ccgtatggtc ggcgaattga tttccaagcc ctatatcgac attactttaa
     1801 aactgatggc gcaattcggc gtacaggttg ccaatgaaaa ctaccgcgtc ttcaaaattc
     1861 ccgccgatgc gcactaccac gcgcccgaac acttgcacgt cgaaggcgat gcctccagcg
     1921 cgtcctactt cctcgcagcc ggtttgattg ctgccacgcc cgtgcgcgtg accggcatcg
     1981 gcgcaaacag catacagggc gatgtcgcct ttgcccgcga gctggaaaaa atcggggcgg
     2041 acgtggtttg gggcgaaaac ttcgtcgaag tttcacgccc gaaggaacgt gccgtccaat
     2101 cctttgattt ggatgcgaac catatccccg atgccgccat gaccctcgcc atcgtcgcgc
     2161 ttgctacagg gcaaacctgc acgctgcgca acatcggttc gtggcgcgtc aaagaaaccg
     2221 accgcatcgc cgcgatggca aacgagttgc gcaaactcgg ggcaaaagtc gtcgaagaag
     2281 ccgaagcaat tcacatcacc ccgcccaaaa cgctgacacc cgacgccgtc atcgacacgt
     2341 acgacgacca ccgcatggcg atgtgtttct cgctggtttc gctgttgggc gtacccgtcg
     2401 tcatcaacga tccgaaatgc acccataaaa ccttcccgac ttatttcgac gtgttctctt
     2461 cactgaccga aacaacggaa taaggcggca ttttgccgcg attccggcgc ggcgcggcgg
     2521 gcggctcatt ctgtaaagaa agtatgtgcg ccgaggtagt ttttggcgta aaacggtgtg
     2581 gagagttttt cggttttgat ggttttgccg ctgctggggg catggatgaa ttcgccgttg
     2641 ccgatataga gtccgacgtg tgagtagcgg tgtgtgccgc cggtgttgaa gaatacgagg
     2701 tcgccggcct taaggcggct gtcggggatt ttgcggcttg ccgccgccat gtcgcgggcg
     2761 gtgcgcggca gcttgacgtt gagggcgttt ttgtaaacga attgaatcat gccgctgcaa
     2821 tcgaagccgg ttgcggtgct gctgccgccc catttgtagg gcgtgccgat gagtccgagg
     2881 ctgtggagca tgagttcctg cgagccttgt gtgcggtcga tgtggctgat gcggacggct
     2941 tggatttgcc ggactgtctg tttgggtttc ggttggcggt gtttgccgga ggtcgtgccg
     3001 catgaggcga ggagcagtgc gctgagacag aggaaaaggg ttttgtcggg gggaaacatg
     3061 gtttttcctt tgcgggttcg gatatccgtc tgaaggt
//
LOCUS       DAWFAD010000186         3078 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWFAD010000186 DAWFAD010000000
VERSION     DAWFAD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.186"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..260)
                     /locus_tag="WGO49_001889"
     CDS             complement(<1..260)
                     /locus_tag="WGO49_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306948.1"
                     /translation="MNMENYFSNLNLDIRTHKLGTFTDQKVTPDVLCAVAECIIEYIE
                     NIGEIFSINDIRYSDYAEYIATAVFKKPSIENAGSEYNKFFS"
     gene            complement(272..2860)
                     /gene="dcm"
                     /locus_tag="WGO49_001890"
     CDS             complement(272..2860)
                     /gene="dcm"
                     /locus_tag="WGO49_001890"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0306949.1"
                     /translation="MKNQYSKSLHGVFYLAFYDKIHAFFAEQLFQLIINMNKKPTYIS
                     LFSSAGVGCYGFKTNGFECVATNELLEKRLKIQKYNQKCRFDSGYIAGDITLPETQAK
                     LFAEIRRWNTEIDVVIATPPCQGMSVANHKKNNEMARNSLVVESIKIVREVQPKFFIF
                     ENVKAFLKTPCTDTDYQEKPIEEAIWSNLGGHYNILSNVLNFKDYGANSSRTRTLVIG
                     VRKDIHHITPYDIFPKQTPPKTLRTLLADLPPLMQMGEISDDDIYHSYREFDRRMLPW
                     IAQLKEGESAFQNTDSARIPHQIKNGEIIYNQNKNGDKYARWFLDREGPCIHTRNDIL
                     ASQNTIHPTENRVFSVRELMRMMSIPPSFKWTAQDEKQLNALSFDEKKTFLKKEELNI
                     RHCIGESVPTAVLANVAQNIQAALQQSELSMTEIQNLIKRENLSLADNLYRFIEQHFD
                     AYPFSRLLQIAEYANAARSETAAYFTRIDIAFGLVNALPDFKKKKNLRILEPSVGIGN
                     FIPLLAKKYADKEKVIFDLVDIDSNSLNLLSLLLKKLNLGSKFEFNIIHQDFLTATLP
                     ENYDLVIGNPPYGKVKASDKNLALYRKNATNKDTQNIFSFFIDKALTLSKTVALVVPK
                     SVINAPEFQITRQQLITANINRIIDYGEKAFKGVKIETIAFIADQTDKDNQHITIESH
                     IQETLIQNCKNYFFSDNFPYWLIYRNSEFDQIAQKLEFNVFQSFRDRQITKAHTQNSG
                     NVRVLKSRNIGNNEIIDIEGYDSYVDSVAPFSVGKFINRKNVVMIPNLTYKPRAAFLP
                     ENSIADGSVALLTMKKHEKILTQRDLAYYATPEFEQFYRVARNYGSRSMNIDNHSVFF
                     FGLLKD"
BASE COUNT          899 a          566 c          607 g         1006 t
ORIGIN      
        1 tgtgaaaaaa atttattgta ttcgctaccg gcattttcaa tggatggttt tttaaatacg
       61 gcggtggcga tatattcggc ataatctgaa taacgaatat cgttaatgct aaatatttct
      121 ccaatatttt caatatattc gatgatacat tctgctacgg cacataatac atccggggtt
      181 actttttgat cggtaaatgt gccgagttta tgtgttctga tgtctaaatt taagttagaa
      241 aaataatttt ccatattcat tttttactcc tctaatcttt tagcaatccg aaaaaaaaca
      301 cagaatgatt gtctatattc attgaacgag aaccataatt acgggcaaca cggtaaaact
      361 gctcaaattc gggagttgca tagtatgcga gatctcgttg ggttaggatt ttttcgtgtt
      421 ttttcattgt cagtagggca accgagccat cagcaatact gttttcaggc aaaaatgctg
      481 cccgaggttt gtaagtcaga ttgggaatca tcacgacatt tttacggtta ataaatttgc
      541 cgacagaaaa aggggcaact gaatccacat aactatcata accctcaata tcaataatct
      601 cgttgttacc gatattacgg gattttaaaa cccgaacatt gccactattc tgtgtatgcg
      661 ctttggtaat ttggcgatct cggaatgatt gaaatacatt aaattccaat ttttgtgcaa
      721 tttgatcaaa ttcactatta cgataaatca accaataggg aaaattatca gaaaaaaaat
      781 agtttttaca gttttgtatc aacgtttctt ggatatggct ttctatcgta atatgttgat
      841 tgtctttgtc ggtttgatcg gcaataaagg caatcgtctc tatttttacg cctttaaatg
      901 ctttctcgcc gtaatcaata atgcggttga tattggctgt gataagttgt tgtcgggtta
      961 tttgaaactc gggggcgtta atcacacttt tgggaacaac caaagccacg gttttactga
     1021 gcgtcagagc cttatcaata aaaaatgaaa aaatattttg cgtatcttta ttggtagcat
     1081 ttttacggta tagtgccaaa tttttatcac ttgccttaac tttgccgtat ggtggattgc
     1141 cgatgactag atcatagttt tcaggcagcg ttgcggttaa aaaatcctga tgaataatat
     1201 tgaactcaaa tttactgcct aaatttaatt ttttcagcag caatgacagg agatttaaac
     1261 tgtttgagtc aatatccacc aaatcaaaaa taactttttc tttgtctgca tatttttttg
     1321 ccagtagtgg aatgaaatta ccaatgccga cagagggttc taaaatccgt aagttttttt
     1381 tctttttgaa atcaggaaga gcattaacca gtccgaaagc aatatcaata cgggtaaaat
     1441 aagcagccgt ttcgctacgg gcagcattag cgtattcagc aatttgcaaa agccgactga
     1501 atggatatgc gtcaaaatgt tgctcaataa atcggtagag attatcggca agggataaat
     1561 tttctcgttt gattaaattt tgaatttccg tcattgacag ttcggactgt tgcaaggctg
     1621 cctgaatgtt ttgtgcgaca ttggccaaaa cagcggttgg cacagattca ccgatacaat
     1681 ggcggatatt cagctcttct tttttcaaaa aggttttttt ctcatcaaat gacagtgcat
     1741 taagctgttt ttcatcttgt gccgtccatt taaatgatgg cggaattgac atcatacgca
     1801 tgagttcacg cacggaaaat acacggtttt cagtgggatg gatggtgttt tggctggcta
     1861 aaatgtcatt acgggtatgg atgcacggac cctcacgatc taaaaaccaa cgggcgtatt
     1921 tgtctccatt tttgttttga ttgtagataa tttcaccatt tttaatttga tggggaatgc
     1981 gtgcagaatc agtattttgg aaagctgact cgccttcttt aagctgtgca atccacggca
     2041 acatacggcg atcaaattca cgatagctat ggtaaatatc atcatcgcta atttcgccca
     2101 tttgcattaa aggcggcaga tcagccagca atgtacgcaa ggtttttggc ggtgtttgct
     2161 tggggaaaat atcgtacggc gtaatgtgat gaatatcttt tcgcacgcca atcaccagcg
     2221 ttctggtacg actggaattt gcaccataat ctttaaaatt tagtacattg gatagaatgt
     2281 tgtaatgtcc gcccaaattt gaccaaatgg cttcctcaat cggcttttct tgataatcgg
     2341 tatcggtgca gggtgttttt aaaaaggctt tgacgttttc aaaaataaaa aacttgggct
     2401 gaacttcccg aacgattttg attgattcca cgaccaaaga atttcttgcc atttcattat
     2461 tttttttatg gttggcaacc gacattcctt ggcaaggcgg tgtggcaatc acaacatcaa
     2521 tttcagtatt ccagcgtcga atttcggcaa acaatttggc ttgggtttca ggcagggtaa
     2581 tatcgccggc aatataacct gaatcaaagc ggcatttttg attatatttt tggattttta
     2641 ggcgtttttc taaaagttcg ttggttgcta cgcattcaaa accattggtt ttaaaaccat
     2701 agcaaccaac gcctgcactg ctaaataaac tgatgtaggt gggttttttg ttcatattta
     2761 tgatgagttg aaacaactgc tctgcaaaaa atgcgtgtat tttatcatag aaggcaaggt
     2821 aaaacacgcc atgcaatgat ttactatatt gatttttcat caaaaaagtg ctgtttgaac
     2881 agatatacag cggtgttttt tgcaatgcgt ttcaaacttg aggtacgttt ttaatagaaa
     2941 caccgtctga acgccaacct gagacctttg caaaattccc acaaatcccc caattcccac
     3001 caagacattt aggggatttc tcatgagcac tttcttccgg caaaccgccc aagccatgat
     3061 tgccaaacac atcgaccg
//
LOCUS       DAWFAD010000187         3073 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWFAD010000187 DAWFAD010000000
VERSION     DAWFAD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.187"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            119..898
                     /locus_tag="WGO49_001891"
     CDS             119..898
                     /locus_tag="WGO49_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0306950.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALTEHPLVGEYGHSFRFLPVTTRAANPSGLSGKRIPELLKNNSIEQALHTKFTPES
                     TRFMICGNPDMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(1108..2520)
                     /locus_tag="WGO49_001892"
     CDS             complement(1108..2520)
                     /locus_tag="WGO49_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0306951.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVVGLTGISDGMVADAPVFAEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRVGIGFSSPVLCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVT
                     DNPPTGLFANKKAARRALSSWAETYGLCPAAAGILSDGYTEDEPCPVYVSDRCDKACV
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDDGLWYFDKD
                     LPDAFKAKFKTDRKNIKEIG"
     gene            2611..2687
                     /locus_tag="WGO49_001893"
     tRNA            2611..2687
                     /locus_tag="WGO49_001893"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2645..2647,aa:Arg,seq:acg)
     gene            2705..2779
                     /locus_tag="WGO49_001894"
     tRNA            2705..2779
                     /locus_tag="WGO49_001894"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2738..2740,aa:Glu,seq:ttc)
BASE COUNT          838 a          917 c          590 g          728 t
ORIGIN      
        1 gtgtttgggt gtcagctgtc gggagggaaa ggaattttgc aaaggtctca gaccgtcaat
       61 gcggaaaaaa acacccaaat tggctataat cccgacaaac acactcaagg acaacaacat
      121 ggcagcctcg cccgaagcaa aattcactga agaaaagatt ttgtgggtca aacaccacac
      181 gccgaaactc atcactttcg ccatcagccg tcccgaatcc taccgtttta aagcgggaca
      241 gttctcccga ctcggtttct acgaagggga aggctttatt tggcgtgcct actccatcgt
      301 ttccgcagaa tatgccgaca cgctcgaata ttttgccgta ctcatccaag acggccccat
      361 gtctgcccgt ttcgccaaaa tgcaacaggg cgacaccatc ctgctcgata aaaatgccac
      421 cggcttcctg cttcccgaac gcttccccga cggcaaggat ttggtgatgc tctgcaccgg
      481 ctccggcatc gcccccttcc tttccattct cgaacaacct gaaatccggc agcgtttcga
      541 taccgtcaac ctgatacatt ccgtatcttt tcccgaagaa ttgattttca acgaccggct
      601 cgccgcattg accgaacatc ccctggtagg cgaatacgga cactcgttcc gtttcctccc
      661 tgttaccacc cgtgccgcca acccctcggg cttaagcgga aaacgcattc cggaactctt
      721 aaaaaacaac agcatcgaac aggcgctgca taccaagttc accccggaat ccacacggtt
      781 tatgatttgc ggtaacccgg acatggtcaa agacactttc caaacgctgc ttgacatggg
      841 ttacgccatg caccgcaacc gcattcccgg tcaaatcatg atggaaaacg gcttctaaaa
      901 accaccctgc ttgtccgatg ccttcggatg gacggtcaaa ccgacacggc acgaaaaccg
      961 cgtcggcaaa aatgccgtct gaaaagttca gacggcatct tcggacacat tacctgcaaa
     1021 cggtaacaca ttccaaccca aaccgattag gcaatcagta cgaacgactg tttacatact
     1081 tgccggcttt caccaaccgg tatcgattta accgatttcc ttaatatttt tcctgtccgt
     1141 tttaaacttc gccttaaacg catccggtaa atctttatcg aaataccaaa gcccgtcatc
     1201 catttctaat gcgccgccca ttccatgcag aacaactttt tcccccgtta aaggatcggt
     1261 ttcggtaatt tccacctcat gcatttttcc ccaatccaag acaggcagct tgtgtgcaaa
     1321 cgccaaaacc tgttcgtcag agcggacgca cgccttatca catcggtctg aaacatatac
     1381 gggacaaggc tcatcctctg tataaccgtc cgacaggata ccggctgcgg caggacacaa
     1441 tccgtatgtt tccgcccacg acgacaaagc ccgtctcgct gcctttttat tggcaaataa
     1501 tccggtaggc ggattatccg tcacaccgtt tttcaaagcc gctgttttcg cattcaacat
     1561 gccgtctgaa cctttttcaa acctgacggt cgtaaatgtt ttaagcagat ttttggcaga
     1621 tacataacaa cccgaatgat aacgtccgac caattccgct ttaatcttat atgcatgcag
     1681 gctgcccaat gcaggaaaaa aacggacttc ctccgtattg caccaatcaa acggggcttt
     1741 tccggaatcc aataaagccg aaacctcgct atatacccgt tcaaacgtac cgatgtaatt
     1801 tacttttcct ccgccgtcga aacaggccag cacccccata ccgtcaggca aaccgtacaa
     1861 ctgttccctc aaccgttcgg gcagcgcggc aggcaacggt ttcggattca tcaaacggaa
     1921 acactgcctg atccatgcct cgaccccgtg ttccgacaga ctgtattcca aataatcgca
     1981 caatgccgat acatccgcca tcgcacgatg cctgtcttcc acaacaatcc ccaacctttc
     2041 gatgatactg tccaggctgt gcttgtaaaa ttgcggatac agacaccggg acagctgcac
     2101 actgcacaaa acaggcgatg aaaatccgat acccacacga tgaaactcat gctttaaaaa
     2161 cgtatagtcg aaacggctgt tatgtgcaac cagcacacaa cccttcaata ccgaaaacaa
     2221 ctcgccggca atctctgcaa aaacaggcgc atcggcaacc atgccgtctg aaatccccgt
     2281 cagccccacc acaaactgcg gaatcggttt ttgaggatta accaaccact catgcctcac
     2341 cacccttccc tgctcaaact tgaccaaagc cacttcggtt accctgtctt catacagatt
     2401 gccgcccgtc gattccaaat caaccacggc aacaggcatt ccaaaccgta aaaatacctt
     2461 ttccagcaag ggccagcgag aagcaacaat cattttattc tctttaaatt caaacaacaa
     2521 accaatattt tacactttta aggcatttca tccaacaaaa caattgacag aatccgatga
     2581 ttaccctaaa attcgaatct ttcttgcagc gcacccgtag ctcagttgga tagagtatct
     2641 ggctacgaac cagagggtcg ggcgttcgaa tcgctccggg tgcgccagta agaaaataca
     2701 atatgcgccc atcgtctagc ggttaggaca tcgccctttc acggcggtaa ccggggttcg
     2761 attccccgtg ggcgtgccaa attctaaatc cccgagatta tcgctcgggg attttttatt
     2821 gtctcagcaa ctcgttacca tatctttacc tacccccttc atcagaatct cagacgtaat
     2881 cgaatcatat tcaaaccttt gccgtgcaaa ccgatatccc ataaccggat gcggtgtccg
     2941 tccaacattt tacccgattg aaacgcctga tatattgcac cccatcaacg tggcattact
     3001 tttcttaaca atcccctttg acagcaactg actagggctt ttttatgcca tcatcaaatt
     3061 tatagtggat taa
//
LOCUS       DAWFAD010000188         2969 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWFAD010000188 DAWFAD010000000
VERSION     DAWFAD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.188"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            123..1667
                     /gene="purF"
                     /locus_tag="WGO49_001895"
     CDS             123..1667
                     /gene="purF"
                     /locus_tag="WGO49_001895"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ0306952.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIVTAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDSEGRKSYAVASESVA
                     FNALTYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDGIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANERSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            1767..2258
                     /gene="greB"
                     /locus_tag="WGO49_001896"
     CDS             1767..2258
                     /gene="greB"
                     /locus_tag="WGO49_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ0306953.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            2317..2943
                     /locus_tag="WGO49_001897"
     CDS             2317..2943
                     /locus_tag="WGO49_001897"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ0306954.1"
                     /translation="MRKIRTKICGITTPEDASAAVAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDTFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT          713 a          909 c          775 g          572 t
ORIGIN      
        1 cttattctcc ataaacccac ctatctgtcc ttcgctcaaa tattgtcaac aattcaggca
       61 aaaaaccacc gtttgcttta taattccgtt ttacccttaa acctcgaatc ggagaagacg
      121 atatgtgcgg cgtattaggt ttggtcagtc atgaacccgt gaaccagctt ctgtacgacg
      181 gtttgcagat gttgcagcac aggggacagg atgcggcggg cattgtaacg gccgaaggcg
      241 gtaccttcca tatgcacaaa ggcaaaggga tggtgcgcga agtgttccgc acgcgcaata
      301 tgcgtgattt gaccggcaac gccggcatcg cccacgtccg ttatcccacg gcgggcaacg
      361 ccggcagcag cgcggaggcg cagcctttct acgtcagctc gccgttcggc atcgttttgg
      421 cgcacaacgg caacctcacc aacactgccg aactttatga aaacgtgtgc aacaaacacc
      481 tacgccacgt caacaccagc tccgattccg aagtcttgct caacgtattc gcgcacgaat
      541 tgcgccgcga agtctctaaa aacgccgacc cgcaccggct caatgccgac aatattttca
      601 acgccgttgc cgaagtccac cgcctggtgc gcggcgcata cggcgtggtt gccatgattg
      661 cgggctacgg tatgcttgcc ttccgcgacc cttacggcat ccgcccgctg gtattgggtt
      721 cgcaaaccga cagcgaaggc agaaaatcct atgccgtcgc ctccgaatcc gtcgccttca
      781 acgcgcttac ctacgatttg gaacgcgaca tccggccggg cgaagcggta tttgtcggct
      841 ttgacggcac aatgattgcc cgccaatgca gcgaccgtgc caaactcagc ccctgccttt
      901 ttgaatatgt ttattttgcc cgccccgact ctgtgattga cggcgtatcc gtttaccaat
      961 cgcgcttgga tatgggcgtg tcgctggcgg aaaaaatcaa acgcgagctg cccgtggacg
     1021 gcatcgacgt tgtgatgccc atccccgaca ccagccgccc cagcgcgatg gagcttgccg
     1081 tccatctcga caaaccctac cgcgagggtt tgattaaaaa ccgctatatc ggccgcacct
     1141 ttattatgcc cggacaggcg acgcgcaaaa aatccgtgcg ccaaaaactc agcccgatgg
     1201 aaaccgaatt tgcaggcaaa agcgtgttgc tggtggacga ctccatcgtg cgcgggacga
     1261 ccagccgcga aatcgtcgaa atggtacgcg cggcgggcgc gcgcaaagtc tatatcgcct
     1321 ccgccgcgcc cgaagtgcgc tatcccaatg tgtacggcat tgatatgccc acgcgcgaag
     1381 agttgattgc caacgagcgc agcgcggcgg aaatcgccgc cgaaatcggc gcagacggca
     1441 tcgtgttcca aaatttgagc gatttggaag ccgtcgtcaa agcactcaat ccgaaaatcg
     1501 aatccttcga ttcgtcctgt ttcaacggca tttatcagac cggcgacatc gatgatgcct
     1561 acctcgaccg cctgtctgcc gagaaatccg gctgcggcgg tctgaaaatc cacccgagca
     1621 ggatggaaca cagcatcagc atcagcgatg cgggcgacga agaataaggc ttcccaaacc
     1681 gcgatgccgt ctgaaatccg aacgccgggt tttcagacgg catttcccat atcggcatca
     1741 catttcccgt ttcatccgca cacaatatga gtaccgaaac caaaaactac attacgcccg
     1801 ccggatggca ggcattgaaa gacgagcttt atcaactggt caacaaagaa cgccccgaaa
     1861 tcgtccaaat cgtcaactgg gcggcaggca acggcgaccg cagcgaaaac ggcgactatc
     1921 tttacggcaa acgccgtatg cgcgaaatcg accgccgcat ccgtttcctg accaagcgtt
     1981 tggagtccgc cgtcgtcgtc gatcccgaat tgcgcgaagc gaccgaccaa gtatttttcg
     2041 gcgcgaccgt cggcctgttg cgcggcgacg ggcgcgagca aaccgtcaaa atcgtcggca
     2101 tcgatgaaat cgataccgcg caaaacaaaa tttcttggat ttcgccgttg gcgcgttgcc
     2161 tgattaaagc gcgcgaaggc gacgaagtgg ttttgaacac gccggaaggg cgtgaggaaa
     2221 ttgaaatcct ttctgtcgaa tacatcaaaa tcgactgatc gggaatgccg tctgaaacgg
     2281 tgcaaacgca tcgtttccca aataaaggaa taccgaatga ggaaaatccg caccaaaatc
     2341 tgcggcatca ccacaccgga agacgcatcg gccgccgtag cggcaggtgc agatgccgtc
     2401 gggctggtct ttttccaagg cagcagccgg gccgtcgata ttgcccgcgc caaaaaaatc
     2461 accgccgcac tgccgccgtt tgtcagcgtt gtcgcccttt tcgtcaacga aagcgcgcaa
     2521 aacatccgcc gcatccttgc cgaagtgccg atacacatca tccaattcca cggcgacgaa
     2581 gacgacacat tctgccgcca gttcgaccgc ccctatatca aggccattcg tgttcagacg
     2641 gcatcagaca tccgaaacgc cgccgaccgc ttccccgacg ctcaggcact gctgttcgat
     2701 gcctaccacc cttcggaata cggcggcacc ggacaccgct tcgactggac gctgctggcg
     2761 gaatattcgg gcaaaccgtg ggtgcttgcc ggcgggctga cccctgaaaa cgtcggcgaa
     2821 gccgtccgca tcaccggagc ggaagcggtc gatgtatccg gcggcgtgga agcgtctaaa
     2881 ggcaaaaaag acccagccaa agtcgccgcc tttatcgcaa ccgccaaccg cctatcccgt
     2941 taaagcaaca aaaattgccg ccggaatga
//
LOCUS       DAWFAD010000189         2938 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWFAD010000189 DAWFAD010000000
VERSION     DAWFAD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.189"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(127..1437)
                     /locus_tag="WGO49_001898"
     CDS             complement(127..1437)
                     /locus_tag="WGO49_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ0306955.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYKQMRRFVAENSSDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLEWLKSLDEEVLKAK"
     gene            1749..2171
                     /locus_tag="WGO49_001899"
     CDS             1749..2171
                     /locus_tag="WGO49_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306956.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASGKKYRQTIVP"
     gene            2257..2754
                     /locus_tag="WGO49_001900"
     CDS             2257..2754
                     /locus_tag="WGO49_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HEZ0306957.1"
                     /translation="MNVKVHKDTHHFGIHNLNNTDVHLTAEDSDNVIEIKGELADLMP
                     TGNVESTSFLRGIETAFKSNTVLTGNNNKISIATEGKGLGLGVNGLQSNSPYSGRTTG
                     IFINNQSSDLTQPSLDGISTIKLKAVKGNNEVDLNVKNHASVKGIIASHSAKATLEAE
                     GDNIV"
     gene            2859..2938
                     /locus_tag="WGO49_001901"
                     /pseudo
     CDS             2859..2938
                     /locus_tag="WGO49_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT          815 a          705 c          697 g          721 t
ORIGIN      
        1 ttaatccact ataccacgtt gtctttattg gctttatcaa taaacaggat aagacctgaa
       61 aaaaagccga tacgcctttt tggtgtaccg gctttgccat actgttctgc ttcagacagc
      121 attgcttcat tttgccttta atacttcttc gtccagcgat ttcaaccatt ccagcttttc
      181 gccgattttg atttccaacc cgcgcgggac gggttggtag aagtccggtt cgtccaagcc
      241 gtcgggcata tagctttcgc cggcggcgta ggcgttcggt tcgtcgtggg cgtagcggta
      301 ttcgcgtccg tagcccaatt ccttcattaa cttggtcggg gcgttgcgaa gatggacagg
      361 cacttcgtcg ctgctgtttt ctgcaacaaa tcggcgcatt tgtttatacg ctttgtagcc
      421 cgcgttggat ttcgcggcgg cggcaaggta caataccgct tgcgccaaag ccagttcgcc
      481 ttcgggcgag cctaagcgtt cgaaggtggc ggcggcatcg ttggcgattt ggaaggcgcg
      541 cgggtcggca agcccgatgt cttcccaagc gatacgcacg atgcggcggg cgaggtagcg
      601 cgggtcggtg ccgccgtcga gcatacggca gaaccaatac agcgcggcgt tcggatgcga
      661 accgcgcacg gatttgtgca gggcggagat ttggttgtag aaactctcgc cgcctttgtc
      721 gaaacggcgg atttgcgccc cgagactgtc ggcgagaaat tcggcggtta agtttttcag
      781 acgacgtgta tcggcggcgc gtaaaagttg ttccaacaaa ttcaacaatc tgcgcgcatc
      841 accgtcggcg gtattcacga gtaatttttg cgcatccgtt tcaatcgtaa actcttggta
      901 ttcaggcaaa gccaatacct tggcaatcag ctttttcagg tcgtctgaag acaagggttg
      961 caaaacatac acctgagcgc ggctcaacag cgcgggattg acttcaaacg acggattttc
     1021 cgtcgtcgca ccaataaagg ttagcaaacc gctttcgaca tgcggcaaaa acgcgtcctg
     1081 ctgcgccttg ttgaagcggt ggacttcatc gacaaacaaa atcgtcgcgc gtccctgctg
     1141 caaagcgatt tcggctttat cgattgcctc gcggatgtcc ttcacgccgg aaaatacggc
     1201 ggaaacaggc aaaaactggg cgttgaaact ctgcgccaaa atccgcgcca acgtcgtctt
     1261 gcccacgccc ggcggccccc acagcaacat agaatgcggc ttgccgcctt ctaccgccac
     1321 gcgcaaaggt ttaccttcgc cgatgaggtg ttcctgcccc accacgtcgt caagcgtatg
     1381 cggacgcaat cgttcggcaa gcggcgcgtc gggttctcgg gcaaacaaat cggtcataac
     1441 ggctccgtca acaggttttc aaacaatatg attatacggc agggaacggc ggcgtgccgc
     1501 atacggattc cgcccctccg tttgccttaa gccgatatta ggcgcatact ggaaaagacg
     1561 agagacttca cacaatatat ccggcacgga gaccgattcc gcatcggcat gacaataccc
     1621 aaatcagcgt ttcaattaaa cattaaggag actaaaatag aaaatttgct ttatctacca
     1681 ttgctttgtt gattcaatcg gcattatgtt ttgaggcgga agcccatgaa tatactaata
     1741 ttcaagagat ggaatgggtg tctttatttt ctgatccgca aagagacgat gatagtctta
     1801 taacccttaa agatgaaaaa atcactgtaa aaaactatat tgtgccttgg tggaaaaaag
     1861 gtgaaaactt tagaaaatta gaacttatta ccactggtaa aaatgataat ggaaatttaa
     1921 cggtaaatgg aatttacggc atccaatctt ttgcagactc tcaattggga tatacgcctg
     1981 aattattggt aaaaagcaat aatgatattc tatttaaaaa taatctccaa tctgttgacg
     2041 tgaaacccca ttccggaaat atccatttgg atgccaaagg aaaaattcaa tttaatttga
     2101 atgttcccga tgttgatggg gtacaagatg tagccagtgg aaaaaaatac cgacaaacaa
     2161 tagtgcctta gtgcggtatg tcaacactat cagccccccg atgaaaatga caaaggcagc
     2221 atatcctttc attcaaaaac aggcaatttg ttcaatatga acgttaaagt tcataaagac
     2281 acacaccatt tcggtattca caacctcaac aataccgatg ttcatctgac tgccgaagac
     2341 agcgataatg tgattgagat taaaggggaa ctcgccgacc ttatgccgac aggaaatgtt
     2401 gagagtacaa gttttttaag gggcattgaa acggctttta aatccaatac cgtattaacg
     2461 gggaataaca ataaaatatc catcgccact gagggcaagg gcttgggatt gggcgtaaat
     2521 ggtctccagt ccaactcccc ctattccgga cgcacgacgg gcattttcat caataatcaa
     2581 tcaagcgacc ttacccaacc ttcccttgat ggcatcagca ctatcaagct caaagccgtc
     2641 aagggaaata atgaagttga tttaaatgta aaaaaccacg cctccgtaaa aggcattatc
     2701 gcgtcgcatt ctgcaaaagc aacgcttgaa gccgaagggg acaatattgt ctaggttaaa
     2761 aatccggaca ccaatacggc tttaatcgat gctttgaaaa aacaataccc caatgcttcg
     2821 aaagaactat acagggtggg ggttcaatcc acgactggaa cagtacgcat ttcgccaaac
     2881 ggcaaaatcg cattaacgga attgaaaact tttggaattg tacaaaacgg catttgcg
//
LOCUS       DAWFAD010000190         2925 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWFAD010000190 DAWFAD010000000
VERSION     DAWFAD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.190"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(199..1188)
                     /gene="pdxA"
                     /locus_tag="WGO49_001902"
     CDS             complement(199..1188)
                     /gene="pdxA"
                     /locus_tag="WGO49_001902"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ0306958.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKHLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAVEAVEAGKLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFCIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            1348..2775
                     /locus_tag="WGO49_001903"
     CDS             1348..2775
                     /locus_tag="WGO49_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0306959.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
BASE COUNT          580 a          800 c          830 g          715 t
ORIGIN      
        1 acctttcgtc attcccgtga aaacgggaat ctagaacctc aaactttcag ataatctttg
       61 aatattgccg tcgcccgaag gtctggattc ccgcctgcgc gggaatgacg atttggaaat
      121 tacccgaaac ccaaaaacaa ctgaaaccga acagaccgga ttcccgcctg cgcggaaatg
      181 acgggtcttt tatcatcttt aaaggctgcc gcgcgccatc tcgacggcgg tctccacggc
      241 agttatcagg ctgccggaat ccgccctgcc ggttgccgcc aaatcgagcg cggtgccgtg
      301 atcgacggag gtgcggataa agggcagccc cagtgtgatg ttcacgccct gcccgaaact
      361 gtggtatttc aacacgggca gcccttggtc gtggtacatc gccaatacgg catccgcacc
      421 ttcgagcata aacggttgga acaaggtatc cgccggatac gggccggcga ggtttatccc
      481 ttcgcggcgc aggttttcga gcgcggggat aatggtgtcg gtttcttcgt gtccgaggtg
      541 tccgccctca ccggcgtggg ggttgagtcc ggcgactagg attttgggat ttttgatgca
      601 gaatttgtgt tttaagtcgt gatgcaaaat gcgtgcgacg ctttcaatca gcggttgcgt
      661 gatggcggcg gcaacgtctt tcagcggcag gtgggtcgtt acgagggcga cgcgcaggcc
      721 tttgccggca agcatcatca cgacctgccc cgtgccgctt ttttccgcca gatattcggt
      781 gtgtccgctg aaaaaacctg tgcttgcgcg cgcgtcgttg atgatgcctt tgtgcagcgg
      841 cgcggtaacg atgccgtcga aaatgccgtc tgaaatgcct gcgagcgcgg tgtccaaaag
      901 ttgcagcaca taggcggcgt tggcgggatt gagtttgccc gcctcaaccg cttcgacggc
      961 agggatgtgc agcacttcca gctcgccgta tgccgcgccg cctgattctg gatcgaagtc
     1021 gcgcaggacg acgcttttgc ccaaggcttc ggcgcgcgcg cgcaacaggt gtttgtcgcc
     1081 caataccgcg cagcggcagg gcaggcgtgc aaacgccaag tccaaacaaa tatcggggcc
     1141 gatgccggca ggctcgccgg aagtaacggc aaaaacaggc tgtttcatcg tgtttgctcc
     1201 aaacaaaatg cgattctaac gccgcagccg cgcggcgatg taaatttttc tgattttgtt
     1261 gacaatctgc tagaatgggc gtttacaaaa tttaaaccct gcttgcatac cgccaatatg
     1321 tgcgagtttc aactttaagg aagcgatatg aacgagaact ttaccgaatg gctgcacggc
     1381 tgggtcggcg ccatcaacga tccgatgtgg tcatacttgg tttatatgct tttgggtacg
     1441 gggcttttct tcaccgtaac cacgggcttt gtccaattcc gcctgttcgg gcgcagcatc
     1501 aaagaaatgc tcggcggccg caaacagggg gacgaccctc acggcatcac gccgtttcag
     1561 gcatttgtaa ccggccttgc cagccgcgtg ggcgtgggca atatcgcggg cgtggcaatc
     1621 gccatcaaag tcggcgggcc gggcgcggtg ttttggatgt gggtaaccgc cttaatcggt
     1681 atgagttcgg cgtttgtcga atcttcgctg gcgcagctct ttaaagtccg cgactacgac
     1741 aaccaccatt tccggggcgg ccctgcctac tacatcactc aagggctggg gcagaaatgg
     1801 ctgggcgtgt tgttcgccct gagcctgatt ttctgtttcg gctttgtgtt tgaagcggtt
     1861 cagaccaata ccattgccga taccgtcaaa gcggcgtggg gttgggagcc tcattatgtc
     1921 ggcgtcgccc tggtgatttt aaccgcgccg attatcttcg gcggcatcag gcgcatatct
     1981 aaagcggcgg aaatcgtcgt ccccctgatg gcggttttgt acctctttat cgcgcttttc
     2041 atcattttga ccaatattcc gatgattccg gacgtgttcg gtcagatttt ttcgggcgcg
     2101 ttcaaattcg acgcggcagc aggcggctta ctcggcggtc tgatttcgca aacgatgatg
     2161 atgggcatca aacgcggcct gtattccaac gaggcgggta tgggttccgc gccgaacgcc
     2221 gccgccgccg ccgaagtgaa acaccctgtt tcgcaaggta tgattcaaat gctgggcgtg
     2281 tttgtcgata ccatcatcgt ttgttcttgc accgccttca tcatcttgat ttaccaacag
     2341 ccttacggcg atttgagcgg tgcggcgctg acgcaggcgg cgattgtcag ccaagtgggg
     2401 caatggggcg cgggcttcct cgccgtcatc ctgtttatgt ttgccttttc caccgttatc
     2461 ggcaactatg cctatgccga gtccaacgtc caattcatca aaagccattg gctgattacc
     2521 gccgttttcc gtatgctggt tttggcgtgg gtctatttcg gcgcggttgc caatgtgcct
     2581 ttggtctggg atatggcgga tatggcgatg ggcattatgg cgtggatcaa ccttgtcgcc
     2641 atcctgctgc tctcgccctt ggcgtttatg ctgctgcgcg attacaccgc caagctgaaa
     2701 atgggcaaag accccgagtt caaactttcc gaacatccgg gcctgaaacg ccgtatcaaa
     2761 tccgacgttt ggtaaatccc gcccttaccg gagccgcttc cccgcgaagc ggcttttccc
     2821 tttccgcaca ctgtaaaaac agggcgaaca agcgtacaat cccaaccctt tacttttgaa
     2881 tccatttcgt ttttcagacg gcatattgaa tatagtggtt caaca
//
LOCUS       DAWFAD010000191         2789 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWFAD010000191 DAWFAD010000000
VERSION     DAWFAD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.191"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            117..440
                     /locus_tag="WGO49_001904"
     CDS             117..440
                     /locus_tag="WGO49_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0306960.1"
                     /translation="MQNETINPKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            554..2731
                     /locus_tag="WGO49_001905"
     CDS             554..2731
                     /locus_tag="WGO49_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0306961.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDLSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          681 a          991 c          697 g          420 t
ORIGIN      
        1 aggcttacat caaagccgat aacgcttccg ttacaactcc gcccactgaa agcagcctgc
       61 aacgaagcca aaacgacaaa ccgcatcgta aaccacccaa cccataggag aaccccatgc
      121 aaaacgaaac catcaacccg aaacagcacc ttgccgccat caaagaatac tggcagcccg
      181 aaatcatcaa ccgccacggg ttccaattcc gcttggtcaa acttttgggc gattacggct
      241 ggcatacgca cggatacagc gacaaagtgc tgtttgccgt ggagggcgac atggcggtgg
      301 acttcgccga cggcggcagc atgacgatac gcgagggcga gatggcggtc gtgccgaagt
      361 cggtgtcgca ccgcccgcgt tcggaaaacg gctgctcgtt ggtgctgatt gagttgtccg
      421 acccgtccga ggccgtctga aaacgaagtt tccgaaggaa gctgagtttc tgcgaagcta
      481 aaagcagcct gcaccttcaa tcaatatgcc gaaaatacaa ccccaccgca caccaacaca
      541 caaaggaaat cccatgacac gcttcaaata ttccctgctg tttgccgccc tgttgcccgt
      601 gtacgcgcag gccgatgttt ctgtttcaga cgaccccaaa ccgcaggaaa gcactgaatt
      661 gccgaccatc accgttaccg ccgaccgcac cgcgagttcc aacgacggct acactgtttc
      721 cggcacgcac accccgctcg ggctgcccat gaccctgcgc gaaatcccgc agagcgtcag
      781 cgtcatcaca tcgcaacaaa tgcgcgacca aaacatcaaa acgctcgacc gcgccctgtt
      841 gcaggcgacc ggcaccagcc gccagattta cggctccgac cgcgcgggct acaactacct
      901 gttcgcgcgc ggcagccgca tcgccaacta ccaaatcaac ggcatccccg ttgccgacgc
      961 gctggccgat acgggcaatg ccaacaccgc cgcctatgag cgcgtagaag tcgtgcgcgg
     1021 cgtggcgggg ctgctggacg gcacgggcga gccttccgcc accgtcaatc tggtgcgcaa
     1081 acgcccgacc cgcaagccat tgtttgaagt ccgcgccgaa gcgggcaacc gcaaacattt
     1141 cgggctgggc gcggacgtat cgggcagcct gaacaccgaa ggcacgctgc gcggccgcct
     1201 ggtttccacc ttcggacgcg gcgactcgtg gcggcagcgc gaacgcagcc gcgatgccga
     1261 actctacggc attttggaat acgacatcgc accgcaaacc cgcgtccacg caggcatgga
     1321 ctaccagcag gcgaaagaaa ccgccgacgc gccgctcagc tacgccgtgt acgacagcca
     1381 aggttatgcc accgccttcg gcccgaaaga caaccccgcc acaaattggg cgaacagccg
     1441 ccaccgtgcg ctcaacctgt ttgccggcat cgaacaccgc ttcaaccaag actggaaact
     1501 caaagccgaa tacgactaca cccgcagccg cttccgccag ccctacggcg tagcaggcgt
     1561 gctttccatc gaccacaaca ccgccgccac cgacctgatt cccggttatt ggcacgccga
     1621 cccgcgcacc cacagcgcca gcgtgtcatt gatcggcaaa taccgcctgt tcggccgcga
     1681 acacgattta atcgcgggta tcaacggtta caaatacgcc agcaacaaat acggcgaacg
     1741 cagcatcatc cccaacgcca ttcccaacgc ctacgaattt tcccgcacgg gcgcctatcc
     1801 gcagcctgca tcgtttgccc aaaccatccc gcaatacggc accaggcggc aaatcggcgg
     1861 ctatctcgcc acccgtttcc gcgccgccga caacctttcg ttgatactcg gcggcagata
     1921 cagccgttac cgcaccggca gctacgacag ccgcacacaa ggcatgacct atgtatccgc
     1981 caaccgtttc accccctaca caggcatcgt gttcgacctg accggcaacc tgtcgcttta
     2041 cggctcgtac agcagcctgt tcgtcccgca atcgcaaaaa gacgaacacg gcagctacct
     2101 gaaacccgta accggcaaca atctggaagc cggcatcaaa ggcgaatggc ttgaaggccg
     2161 tctgaacgca tccgccgccg tgtaccgcgc ccgtaaaaac aacctcgcca ccgcagcagg
     2221 acgcgacctg agcggcaaca cctactaccg cgccgccaac caagccaaaa cccacggctg
     2281 ggaaatcgaa gtcggcggcc gcatcactcc cgaatggcag atacaggcag gttacagcca
     2341 aagcaaaacc cgcgaccaag acggcagccg cctgaacccc gacagcgtac ccgaacgcag
     2401 cttcaaactc ttcactgcct accactttgc ccccgaagcc cccagcggct ggaccatcgg
     2461 cgcaggcgtg cgctggcaga gcgaaaccca caccgaccct gccacgctcc gcatccccaa
     2521 ccccgccgcc aaagcccgcg ccgccgacaa cagccgccaa aaagcctacg ccgtcgccga
     2581 catcatggcg cgttaccgct tcaatccgcg cgccgaactg tcgctgaacg tggacaacct
     2641 gttcaacaaa cactaccgca cccagcctga ccgccacagt tacggcgcac tgcggacagt
     2701 gaacgcggcg tttacctatc ggtttaaata aggtcgtctg aaaacggagt ttctgcgaag
     2761 ctatagtgga ttaacaaaaa ccagtacgg
//
LOCUS       DAWFAD010000192         2730 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWFAD010000192 DAWFAD010000000
VERSION     DAWFAD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.192"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1..428
                     /locus_tag="WGO49_001906"
                     /pseudo
     CDS             1..428
                     /locus_tag="WGO49_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(475..1884)
                     /gene="dacB"
                     /locus_tag="WGO49_001907"
     CDS             complement(475..1884)
                     /gene="dacB"
                     /locus_tag="WGO49_001907"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ0306962.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDIADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVAKNI
                     ISGGDGWLDAKLMCKERRA"
     gene            2049..2615
                     /locus_tag="WGO49_001908"
     CDS             2049..2615
                     /locus_tag="WGO49_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0306963.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          653 a          643 c          750 g          684 t
ORIGIN      
        1 aacgtggtcg cggtgctgcc ggtaaagtcg ccgtattcgg tcttttgaag cgaaatggta
       61 aggtttatac ggttacagta ccgaatactc aaaccgctac tttatttcct attatccgtg
      121 aacaagtgaa acctgacagc attttttgta gctatgatgt attagatgtg cgcgaattta
      181 gccattttag cttcgctgaa acttcgtttt cgtatcaatc acagcacaca ttttgccgaa
      241 cgacaaaacc atattaatgg aattgagaac ttttggaatc aagcaaaacg tcatttacgc
      301 aagtttaacg gcattcccaa agcgcatttt gagctgtatt taaaggagtg cgaatggcgt
      361 tttaacaaca gtgagataaa agttcttgtt ccatcttaaa acaattagta aaaccgagtt
      421 tatcctagtt atctaggaca gccccttaaa tttaatccac tatattttcc tgtttcaggc
      481 tcggcgttct ttgcacatca gtttcgcatc cagccagccg tcgccgccgg agatgatgtt
      541 tttggcaacg aagttgtcca agtcgggcag cagggaaacg gcgcggccgc tgttgatgat
      601 gacgaccacc gccatcggtt tgtcgcccag ccaataacct gcaagggcgc ggacattgtt
      661 gagcgtgccg gtttttaagc gcaacagccc gccgctttgt ttgaagcggt tgcgtaaagt
      721 tccgtctgtg ccggcgatgg gcagcgtgtc gatgaaatct tgtgcaaacg ggctgaaata
      781 agccgtttcc aacatttgcg ccatcattct cgccgttacc ctttcttttc tggacagacc
      841 tgaaccgttt tccaaaacca aatccgcaat atcgatgccc gacacggcaa gttcgcgccg
      901 gacggcagac gccgcctgtt cggaaacggc gggcagtttg ccgtcgccgc cgagtttgag
      961 gaagacggaa cgcgcaatta gattgtccga acgcttgttc atgtccgtca aaatttcctt
     1021 catcggcttt gagtgtgcaa cggcaagcgt ctgcgcgcct tccggcgtgt cggatatgcc
     1081 gatgccgtct gaaatccgtc cgccgccgag cagccagtgg ttggtaaaac tttgccggat
     1141 cagttcgtca agcgcgaaca tccggacacc gacaggcttg cccaaacagc tctcgggaat
     1201 attgccgcgc aatttcagcg tattgtccga aaaagatgca cgcatcagtt ttttgatcga
     1261 agggcaggca gcttgggagg cggtaatttt caagttgttt tgggcgaaaa tatgcggcaa
     1321 aggcggatcg gtgaggatgt cggtactgtc ggcggcattg cgttcggcgc gcaccataac
     1381 cataccggca gacagcatag ttggattggg gggcgtcata aacggcgaac cgctgtcggc
     1441 ttcgaaatcg tcggggctgc cgacttcgcc ccacaggctg tggtcgagca tcaggtgtcc
     1501 cgtgatattg cgtatgcctt gttcgcgcaa ctggcgttgg acggcaagca ggttttcctg
     1561 attgaaaacg gggtcgccgc tgcccgccca atacaggttt ccgtcaagcg tgccgtcgtt
     1621 taccgtaccg ttgcttttaa actcggtcgc ccagcggtaa ttgctgccga aggttttgaa
     1681 ggcggcaaac gcggtaacga gtttcattgt ggaggcgggg ttgacgggga catccgagcg
     1741 gtggtcaatg atgacttttc cgctgtcaag ctcttggaca tatacggcga tttcgttttg
     1801 cggaatgcga cctgtatcga gcgcgtgtgc ggcgagggag gcgagaagca gcagcaggga
     1861 ggccgctgtt ttggggaaat tcataggtaa atctgttaca taaaaaaagc aattataagg
     1921 caaagccgga taagtgggaa cgctgtggcg gcgggccggc ttgtttgcag gggaaatcac
     1981 atatataata atcgctatca ttatgaaatg attgagacgc acaaacttaa tattcaggag
     2041 gaatgattat ggctaagaaa atcagtattt tggtgggcag cctgcgccgt gcttcttttg
     2101 cgcgcaaagt ggcattgaat gcggcggaga tgttccccga aggctggcag gcggaaatcg
     2161 tcgaaatcgg acatctgccg ctttacaatt tcgattatga cgaccctgcg gtggaagatg
     2221 tgccgctgcc cgaaagctac acggttttcc gcgaaacgat taaggcttcg gacggcattt
     2281 tgttcgttac gtccgaaaat aaccgcacca ttcccgcctg tttgaaaaat gcggtggaca
     2341 tcggctcgaa accgaatgcc gacgtggcgt ggaaaaacaa accggccggc atcatcagcc
     2401 attccgtcgg caagatgggc ggttacagtt cgcaaaaaaa cctgcgcctt gcgctgtctt
     2461 atttcgatat gcccgtaacc ggacagccgg aagtgttttt gggcaattcg ccgacactgt
     2521 ttgatgaaaa cggcaaattg atcgactcgg caagggattt tgttcagtca tatattaatc
     2581 agtttgtcgg tttgattgaa agaaatgcca aataaacaaa caagtcgaaa ggccgcaaaa
     2641 ccggttcggg ttttgcggct tttttatatg ggctgggcag cgtcaaggtt tgccgtttgc
     2701 cggataatgc gcggcaaatg accggcgtgt
//
LOCUS       DAWFAD010000193         2625 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWFAD010000193 DAWFAD010000000
VERSION     DAWFAD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.193"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            121..750
                     /locus_tag="WGO49_001909"
     CDS             121..750
                     /locus_tag="WGO49_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ0306964.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPILLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            896..2557
                     /locus_tag="WGO49_001910"
     CDS             896..2557
                     /locus_tag="WGO49_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ0306965.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGHADFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPTVHFLNPAAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENI"
BASE COUNT          514 a          667 c          684 g          760 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt taatccacta tattttgacg gggttgaagt
       61 tgacatctac aaggtttaat gtttgaatac atttaaaaat atatttgaat gagatgtttt
      121 atgattcaaa atgtcgttac ttcaataatc ctgtattctg ggacagccgt agacttactt
      181 attatcctaa tgttattttt tgccaaaaga aaaagtagaa aagacatcat taacatctat
      241 ttaggacaat ttctaggctc tgttagtctg atattgctaa gtttactttt tgcatttgtc
      301 ttagattata ttcctagtaa agagatttta ggtttgctcg gcttgattcc aattctccta
      361 ggcatcaaag ttttgctttt aggagattct gatggagagg ctattgccaa agagggtttg
      421 cgcaaagata ataaaaacct gatttttcta gtcgctatga ttacttttgc aagttgtggt
      481 gctgacaata ttggtgtctt tgtcccatat tttactacct taaatttagc gaatttgata
      541 gtggctttac ttacctttct agtcatgatt tatctcttgg ttttttctgc ccaaaaatta
      601 gcacaagtcc cttctgttgg agaaactttg gaaaaatata gcagatggtt tgttgctgtt
      661 gtttatttgg gattgggggt atatatcctg attgaaaaca acagttttga tatgctatgg
      721 actgtgtcgg gccaggaaaa aatattatga aaaagatagt atctgcaaag actgccatgt
      781 gcagtctttt ttgttgccgg tctttttgtg tctgatgccg tctgaagcag tctctgcacg
      841 acctttgtgc gaatatttgc tacacttggc aatccgtcca ttttttctca cgcttatgct
      901 gacctacact ccgcccgatg cccgcccgcc cgccaaaacc cacgaaaagc cgtggctgct
      961 gctgttgatg gcgtttgcct ggttgtggcc cggcgtgttt tcccacgatt tgtggaatcc
     1021 tgccgaacct gccgtctata ccgccgtcga agcactggca ggcagcccca cccccttggt
     1081 tgcccatctg ttcgggcatg ccgatttcgg cataccaccc gtgtatcttt gggttgccgc
     1141 cgcgttcaaa catttgctgt cgccgtgggc agccgacccg tatgatgccg cacgctttgc
     1201 aggcgtgttt ttcgccgttg tcgggctgac ttcctgcggc tttgctggct tcaacttttt
     1261 gggcagacac cacgggcgca gcgtcgtcct gattctcatc ggctgtatcg ggctgattcc
     1321 gaccgtacac tttctcaacc ccgctgccgc cgcctttgcc gccgccggac tggtgctgca
     1381 cggttattct ttggctcgcc ggcgcgtgat tgccgcctct tttctgctcg gtacgggctg
     1441 gacgctgatg tcgttggcag cagcttatcc ggcagcattt gccctgatgc tgcccttgcc
     1501 cgtactgatg tttttccgtc cgtggcaaag caggcgtttg atgttgacgg cagtcgcctc
     1561 acttgccttt gccctgccgc ttatgaccgt ttacccgctg ctcttggcaa aaacgcagcc
     1621 cgcgctgttc gcgcaatggc tcgacgatca cgttttcggt acgttcggcg gcgtgcggca
     1681 cgttcagacg gcattcagtt tgttttacta tctgaaaaac ctgctttggt ttgcactgcc
     1741 cgcgctgccg ctggcggttt ggacggtttg ccgcacgcgc ctgttttcga ccgactgggg
     1801 gattttgggc gtcgtctgga tgcttgccgt tttggtgctg cttgccgtca atccgcagcg
     1861 ttttcaggat aacctcgtct ggctgcttcc gccgcttgcc ctgttcggcg cggcgcaact
     1921 ggacagcctg aggcgcggcg cggcggcgtt tgtcaactgg ttcggcatta tggcgttcgg
     1981 actgtttgcc gtgttcctgt ggacgggctt tttcgccatg aattacggct ggcccgccaa
     2041 gcttgccgaa cgcgccgcct acttcagccc gtattacgtt cccgacatcg atcccattcc
     2101 gatggcggtt gccgtactgt tcacaccctt gtggctgtgg gcgattaccc gcaaaaacat
     2161 acgcggcagg caggcggtta ccaactgggc ggcaggcgtt accctgacct gggctttgct
     2221 gatgacgctg ttcctgccgt ggctggacgc ggcgaaaagc cacgcgccgg tcgtccggag
     2281 tatggaggca tcgctttccc cggaattaaa acgggagctt tcagacggca tcgagtgtat
     2341 cgacataggc ggcggcgacc tgcacacgcg gattgtttgg acgcagtacg gcacattgcc
     2401 gcaccgcgtc ggcgatgtac aatgccgcta ccgtatcgtc cgcttgcccc aaaatgcgga
     2461 tgcgtcgcaa ggctggcaga cggtttggca gggtgcgcgc ccgcgcaaca aagacagtaa
     2521 gttcgcactg atacggaaaa tcggggaaaa tatataaaaa acaacagatt gagccgaatt
     2581 tctggattaa gtgccggaaa tcgcgtataa ttgcgcgatt aaacc
//
LOCUS       DAWFAD010000194         2611 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWFAD010000194 DAWFAD010000000
VERSION     DAWFAD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.194"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..453
                     /locus_tag="WGO49_001911"
     CDS             <1..453
                     /locus_tag="WGO49_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ0306966.1"
                     /translation="ITATAQTALSVAEAAGTVWGGKKVELNPTKWDWVKNTDYKTPAA
                     RPMQTLDGEMAGGNKPVVKSIRPTTRDELRQALQEQGFRRTGSDAAQYETWKGPDGVK
                     IDIRPNGEVIRTQRVPRTDGVQGKYPQRQDYEGNPLPNNHHHSGYFVK"
     gene            450..767
                     /locus_tag="WGO49_001912"
     CDS             450..767
                     /locus_tag="WGO49_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306967.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            837..1505
                     /gene="mafB"
                     /locus_tag="WGO49_001913"
                     /pseudo
     CDS             837..1505
                     /gene="mafB"
                     /locus_tag="WGO49_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            1515..1721
                     /locus_tag="WGO49_001914"
     CDS             1515..1721
                     /locus_tag="WGO49_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306968.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            1853..2104
                     /locus_tag="WGO49_001915"
     CDS             1853..2104
                     /locus_tag="WGO49_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306969.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            2101..2472
                     /locus_tag="WGO49_001916"
     CDS             2101..2472
                     /locus_tag="WGO49_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0306970.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
BASE COUNT          924 a          451 c          533 g          703 t
ORIGIN      
        1 ataacagcaa cagcccaaac tgccctttcc gtagcagagg ccgcaggtac ggtttggggc
       61 ggtaaaaaag tagaacttaa cccgactaaa tgggattggg ttaaaaatac cgattataaa
      121 acacctgctg cccgacctat gcagacttta gatggggaaa tggctggtgg aaacaagcca
      181 gttgttaaat ctatcagacc aactacgcga gatgaattac gtcaagcatt gcaagaacaa
      241 ggttttagac gtactggttc agatgcggct caatatgaaa catggaaagg tcctgatggc
      301 gtgaaaatag atattcgtcc aaatggagag gttataagaa cccaaagagt gccgcgaacc
      361 gatggtgtac agggaaaata tccgcaacga caagattatg aaggcaatcc attgccaaat
      421 aatcatcatc attctggata ttttgtcaaa tgaaaaaaaa tatttttcac aatgtaagcc
      481 tttatgaaat aatcttttcc gataatggaa atacccttac attatctttt acagatacaa
      541 ttgaaggtaa ttatttcgga tatatcaaat gcagtaatat tttgaatttt aaattagata
      601 caaataattt cgtagattat gaggataagg aagatagctt gtttcccttg tttatacccg
      661 aaatagagct atataaatac caattttata gtgaaattat tattgatgta gggattatta
      721 taaaaatatc tgctgaaaca attaattttg agccactggg aaaatagtaa ctgctttctc
      781 agcagccgta gcaactgtat ttttacccga cggggtaaaa atacagttgc tacatctctg
      841 cgcagcagac tctacaaggt attaataatt taggaaattt aagtccagaa acacaacttg
      901 ctgccgcgag cctattacag gatagtgctt ttgcggtaaa agacggcatt aattccgcca
      961 gacaatgggc tgatgcccat ccgaatataa ctgcaacagc ccaaactgcc cttgccgtag
     1021 cagaggccgc aactacggtt tggggcggta aaaaagtaga acttaacccg accaaatggg
     1081 attgggttaa aaataccggc tataaaaaac ctgctgcccg ccatatgcag actgtagatg
     1141 gggagatggc aggggggaat agaccgccta aatctataac gtccaacagc aaagcagatg
     1201 cttccacaca accgtcttta caagcgcaac taattggaga acaaattagt agtgggcatg
     1261 cttataacaa gcatgtcata agacaacaag aatttacgga tttaaatatc aattcaccag
     1321 cagattttgc tcggcatatt gaaaatattg ttagccatcc aacaaatatg aaagagttac
     1381 ctcgcggtag aactgcgtat tgggatgata aaacagggac aatagttatc cgagataaaa
     1441 attctgacga tggaggtaca gcatttagac caacatcagg taaaaaatat tatgatgatt
     1501 tataggaaaa agccatgaat atactatcca taaataatca aaactcaact atttcactaa
     1561 ctcaagatga agtttttgtt ttacgagcta tcttgaatga gatatatgcg ggcgtatgtg
     1621 tagattcaag agaatttgaa aatgtatctg gtgttagaaa acatgaagta gataatttac
     1681 aacaacagtt tgctggaatt tataaaaaaa tgacaactta acaacccaaa tttttgtcag
     1741 agcctagtgc aaattacaac tatgattcta ttgtagccga aatgaaagaa aaaaatcatg
     1801 ggttggcgac agggttgatg ttgttaatat gcctgatgga gcacctacta gtatggataa
     1861 cacgcgtatt atggcagcac gtgaagcagg ggtaaaagtg gaagcgaatg ttcataattt
     1921 taatgaccga ttatcatcaa aagagagaat caggtttaag catgatggta ttgagcctca
     1981 aacttgggga gaagctatcc agctacgaat tagaaagcaa gaaacacaaa aaggagttcc
     2041 agaagggtgg agcaaaagat ttcctaacgg aagtatttat gatgtaaagg tacttaggaa
     2101 atgataaaac aaaatagttt tgttccgtat cctgaagcaa tgcttcctaa aggatttaaa
     2161 tatccgcaaa gttatttaaa attagctcaa tccactcatg ccattaacta cgatgaacaa
     2221 tattcttttc cttggtggtt tgaaaatgca gaaagcaata tatcagaagt aattgacatt
     2281 tattttgaaa taactggcat tccaaaccta ttaccttttg ctagaaacca agagtgggct
     2341 gcctgttttg atatttcaga taaatcaggt aatcctaaaa ttatagtagt taatttagat
     2401 aatacaaaat attacgagac ttttgaaaat tttgatactt ggctaaaaga agctgaaaat
     2461 gatggttggt agcagccgta ggtcggattc tcgaatccga cattttccaa cagcggcatt
     2521 tcggaaacga tagacgcgtc aaatattttt gtcggataca agtatccgac catacggcaa
     2581 tcatacgggt aactctgccc catccgtaga g
//
LOCUS       DAWFAD010000195         2565 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWFAD010000195 DAWFAD010000000
VERSION     DAWFAD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.195"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(12..182)
                     /locus_tag="WGO49_001917"
     CDS             complement(12..182)
                     /locus_tag="WGO49_001917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306971.1"
                     /translation="MAIMAYFGGFVRALFDLGVFERGGVKGRGKWFSRVWRRSEVLCL
                     MCGGIFSVKLIL"
     gene            242..2467
                     /locus_tag="WGO49_001918"
     CDS             242..2467
                     /locus_tag="WGO49_001918"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ0306972.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTSDIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVIVPEATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRTAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKNGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAEGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNGKSPKRKAGELDNRGSHFYLTLYWAQELAEQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAVDIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT          711 a          775 c          593 g          486 t
ORIGIN      
        1 aatttaaacc actataaaat caatttcacc gaaaatatgc cgccgcacat caggcacagc
       61 acctccgacc gccgccaaac gcgggaaaac catttccccc ttcctttcac gccgccgcgt
      121 tcaaacacgc caagatcgaa caaagcgcga acaaagccgc caaaatatgc cataatagcc
      181 acgcacattc cccgcgcaat gtgttccaaa agaaaaaacc aacctacaag agaataatcc
      241 tatgactcaa aaatccacca ttgtttatac ccataccgac gaagcacccg cgctggcgac
      301 ccaatcgctg ctgccgattg tgcaggcttt tgcccgccac gccgatattg atgtcaaaac
      361 cagcgacatt tctctctccg gccgtatttt ggcggcgttt cccgaatacc tgaccgaagc
      421 gcaacgcgta cctgatgcgc ttgccgaatt gggcgaactg gtgaaacaac ccgatgcaaa
      481 cgtaatcaaa ctgccgaaca tcagcgcgtc cgtaccgcaa ctgactgccg cgattaaaga
      541 attgcaatct aaaggctttg ccgttcccga ctatcccgcc gacccgcaaa ccgatgaaga
      601 aaaagccgta cgcgaacgct acgaccgcat caaaggcagc gcggtaaacc ctgtcctgcg
      661 tgaaggcaac tccgaccgtc gcgcccctaa agcagtgaaa aactttgcga aaaaaaatcc
      721 gcacagcatg ggcgcatgga ccaaagactc caaaacccac gttgccacca tgcaaagcgg
      781 cgactttttt cataacgaac aatctgttat cgtacctgaa gcgacttccg tatccatcgt
      841 attcaccgac aaacaaggca acaaaaaaga gctgcgcgag cccgtcgccc tgaaagccgg
      901 cgaaatcatc gacgcgaccg tgatgagtaa aaaagccctg ctcgccttcc ttgccgaaca
      961 agtaaaagat gcgaaagcaa aaggcgtgtt gttctcgctg cacatgaaag ccactatgat
     1021 gaaagtgtcc gacccgatta tcttcggaca cgccgtcaaa gtattcttcg cgcctgtttt
     1081 tgaaaaattc ggcgacaaac tggctgccgc aggtgtcaac gtcaacaacg gcttcggcaa
     1141 cctgcttgcc aatctggaca aactggatgc ggacacccgc accgccgtcg aagccgaaat
     1201 cgccgccgtt tacgctgcca accccgattt ggcgatggtt gattccgaca aaggcatcac
     1261 caacctgcac gttcccagcg atgtcatcgt cgatgcctct atgcctgcga tgattcgcaa
     1321 ttccggccgt atgtgggaca aaaacggcaa agcgcaagac accaaagccg tgattcccga
     1381 cagcagctat gccggcgttt accaagcaac catcgacttc tgccgcgaac acggcgcatt
     1441 tgacccgaca accatgggta ctgtgcccaa cgtcggactg atggcgcaag cggcggaaga
     1501 atacggctcg cacaacaaaa cctttgaaat cgaagccgac ggtcaggttc aagtcattga
     1561 tgcggaagga aaagtcctga tgcaacacga cgttgaagca ggcgacatct ggcgtatgtg
     1621 ccaaaccaaa gacgctccgg tcaaagactg ggtgcaactc gccgtcaacc gcgcccgcct
     1681 gagcaacacg cctgccgtgt tctggctcga tgaaaaccgt ccgcacgaca agagcctgct
     1741 cgccaaggtt aaagcctacc ttgccgaact ggataccaat ggcctcgaca tccgcgtcct
     1801 cgctcctgaa gaagccgcca agttcagctt gggtcgtctg aaaaacggcg aagacaccat
     1861 ctccgtaacc ggtaatgtct tgcgcgacta cctgaccgac ttgttcccaa ttttggaact
     1921 cggcacaagc gcgaaaatgc tgtctatcgt tccattaatg aacggcggcg gtatgtttga
     1981 aaccggcgcg ggcggttctg caccgaaaca cgttcaacag ttcctcgaag aaaaccacct
     2041 gcgttgggac tctttgggcg aattcctcgc actcgccgta tcgtttgaac atttggcgca
     2101 aaaaaccggc aatgccaaag cccaagtcct cgctgacact ttggatgccg ccaccgaaaa
     2161 actgctgttg aacggcaaat cgcctaaacg taaagccggc gaactcgaca accggggcag
     2221 ccatttctac cttaccctct actgggcgca agaattggcg gagcaagaca aagatgccga
     2281 actgaaagcc gcatttacgc cattggcagc cgctttgacc gccgacgaag cgaaaatcgt
     2341 tgccgagctt tccgccgtac aaggcaaagc ggtcgacatc ggcggctact acgcagccaa
     2401 tcctgaaaaa gcggcacaag tgatgcgtcc gagcgtaacc tttaatcagg cactaaacgc
     2461 cttataagca caaaggtaaa aatgccgtct gaacattcgt cgttcagacg gcattagtta
     2521 tccctatctg cctgattatg attaaacgcc gcacccattg gatat
//
LOCUS       DAWFAD010000196         2504 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWFAD010000196 DAWFAD010000000
VERSION     DAWFAD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.196"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            168..791
                     /gene="lolA"
                     /locus_tag="WGO49_001919"
     CDS             168..791
                     /gene="lolA"
                     /locus_tag="WGO49_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ0306973.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTKPYRQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1064..2203
                     /locus_tag="WGO49_001920"
     CDS             1064..2203
                     /locus_tag="WGO49_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEZ0306974.1"
                     /translation="MKKTLVAAAILSLALTACGGGSDTAAQTPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEQETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKAL
                     GTDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARELM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2337..>2504
                     /locus_tag="WGO49_001921"
     CDS             2337..>2504
                     /locus_tag="WGO49_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0306975.1"
                     /translation="MNLSIVVPIYNVESYLEACLSSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKI"
BASE COUNT          682 a          731 c          620 g          471 t
ORIGIN      
        1 gcggcacaca ttaaagggca gcgtggcgcg cctgcctttt ccggtgggca aaaaatcggc
       61 cctcggaaaa cgctgtttgt aaaatgcaaa ccgcccgtaa cgccgcccgt atgattgttt
      121 tgccgcgccg atactttacg ccacactcat cccgacaagg aaaaataatg atgaaaccgc
      181 acaacctgtt ccaattcctc gccgtttgct ccctgaccgt cgccgtcgct tccgcacagg
      241 cgggcgcggt agacgcgctt aagcaattca acaacgatgc cgacggtatc agcggcagct
      301 tcacccaaac cgtccaaagc aaaaagaaaa cacaaaccgc gcacggcacg ttcaaaatcc
      361 tgcgaccggg ccttttcaaa tgggaataca ccaaacctta caggcaaacc atcgtcggcg
      421 acggtcaaac cgtttggctc tacgatgttg atctggcaca agtgaccaag tcgtcccaag
      481 accaggccat aggcggcagc cccgccgcca tcctgtcgaa caaaaccgcc ctcgaaagca
      541 gctacacgct gaaagaggac ggttcgtcca acggcatcga ttatgtgctg gcaacgccca
      601 aacgcaacaa cgccggctac caatacatcc gcatcggctt caaaggcggc aacctcgccg
      661 ccatgcagct taaagacagc ttcggcaacc aaacctccat cagtttcggc ggtttgaata
      721 ccaatcccca actctcgcgc ggcgcgttca agtttacccc gcccaaaggc gtggacgtgt
      781 tgagcaactg atgccgtccg ccccgatgcc gtctgaaagc cgccgaggct tcagacggca
      841 ttttcacgca ggcggaacaa tgtcccgcat taccgcccga tcgggcatcg gaacggcaaa
      901 cccgtgaaaa ttaacggttg cgccccggtt gtttttgccg tttaatgcaa accttgctgc
      961 atcaagggcc aagaaagccg accggccgcc cccacagctt ccgatgcagg cggccaggcc
     1021 gtccctgcaa tgttttttat ttttgaacga aaggtcgaaa accatgaaaa aaacactggt
     1081 ggcggcggca atcctgagcc tcgccttgac tgcgtgcggc ggcggaagcg ataccgccgc
     1141 ccaaaccccc tccgccaagc ccgaagccga acaatcgggc aaactcaata tctacaactg
     1201 gtcggattat gtcgatcccg aaaccgtcgc cgcctttgaa caagaaaccg gcatcaagac
     1261 gcgttccgat tattacgaca gcaacgaaac actggaggca aaagtcctga ccggcaaatc
     1321 cggctacgac ctgaccgcgc cgtccatcgc caacgtcggc cggcaaatca aagcgggcgc
     1381 gtatcagaaa atcgacaagg cgcaaatccc ccattacggc aacatcgata aagatttgct
     1441 gaaaatgatg gaagccgtcg atccgggcaa cgaatacgcc gtcccctatt tctggggcat
     1501 caacaccttg gcaatcaaca cgcggcaggt gcaaaaggca ttgggtacgg acaagctgcc
     1561 cgaaaacgaa tgggatttgg tgttcaaccc cgaatacacc gccaaactca aatcctgcgg
     1621 catcagctat ttcgacagcg caatcgaaca gattcccttg gcgttgcact atttgggcaa
     1681 agaccccaac agtgaaaatc ccgaagacat caaagccgcc gtcgatatga tgaaagccgt
     1741 ccgaggcgac gtgaaacgct tcagctcttc cggctatatc gacgatatgg cggcgggcaa
     1801 cctgtgtgcc gccatcggtt acggcggcga tttgaacatt gccaaaaccc gtgccgaaga
     1861 agccgcaaac ggcgtggaaa tcaaagtatt gaccccgaaa accggcgtgg gcgtgtgggt
     1921 ggattccttt atgattccgc gcgacgcgca aaacgtcgcc aacgcccacc gctatatcga
     1981 ctacacgctc cggcccgagg tggcggcgaa aaacggcagc ttcgttacct acgcgcccgc
     2041 cagccgtccc gcgcgcgagc tgatggatga aaaatacacc tccgacgcat cgattttccc
     2101 gaacaaagaa ctgatggaaa aaagtttcat cgtatcgccc aaatccgcag aatccgccaa
     2161 actgggcgtg aagctgtggc aagggctcaa ggccggaaaa taaccgcaac ccctgccgtc
     2221 tgaaaccttt cgggcggcag gaaacggcgc gtccgttatc aaacaggggg ggcgtttccc
     2281 ctcctgccgg ttatgattgg gttaagatta aaatgattta gtaaaatgag aaagatatga
     2341 atttaagtat cgtagttccc atttataatg tcgaaagtta tttggaagcg tgtttaagtt
     2401 ccatagaacc tatattaagt aatgaaaatg tcgaacttat ccttgtgaat gacgggtcaa
     2461 aagacggaag tgaagatata tgttataaat atatagataa aata
//
LOCUS       DAWFAD010000197         2504 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWFAD010000197 DAWFAD010000000
VERSION     DAWFAD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.197"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(13..819)
                     /locus_tag="WGO49_001922"
     CDS             complement(13..819)
                     /locus_tag="WGO49_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ0306976.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGRGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRSF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1039..2409
                     /gene="ffh"
                     /locus_tag="WGO49_001923"
     CDS             1039..2409
                     /gene="ffh"
                     /locus_tag="WGO49_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ0306977.1"
                     /translation="MLDNLTGRFSNVFKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVAGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
BASE COUNT          693 a          630 c          652 g          529 t
ORIGIN      
        1 tgttggctct tcttatcttt tcagaatgat ttccaatacg aacttgctgc ccatataggc
       61 aatcataagg ctgacaaatc cgatgatggt ccacacggcg gcttttttac cgcgccatgc
      121 ggtcatgctg tgcttgagca gcagtccgcc gtaaatcagc catgacaata tgccgaatac
      181 ggttttatgg gtaaaggtca tgggtttgcc gaatacggct tcggcgaaga atgtgccgct
      241 gacgacggaa taggtcagca ggatgaaacc tgcccacatg gcctggaaca tgagtttttc
      301 caaactgagc agcgacggca ggaatcctgc gagcttggag aagctcctgc ggtgcaggct
      361 ccgattcagc agcagggtca aaacggacaa taatgttgcg atgccgaaca gcccgtatgc
      421 aagcagcgaa gttccgatat gcagcataaa gggaaggtcg gtaatttcat atcccgagaa
      481 ttttccagga aaaaccaaac ctgacagcag catcagtgcg gcgcaaggat acagcagcaa
      541 ctgcactccg cgcagcggat aaaagaagct gccggcaaaa taaataaaca gcatcatcca
      601 aacaatcagg ctgccggaat acccgaagcc cataatgatg attttgtctt gaatgaccgg
      661 cataagcagt gccgcgccgt ggacggtcaa tgccgcgccc aaaaccggca attccgtctt
      721 ccacgggtaa tcccggccgc gcccctgctg ttggcagtgc catgcaaatg cacccaatcc
      781 tgcgtaaacc gccgtcaaaa agatgaaaac tgtcggcatg gtggactttc tctatctata
      841 ctgttgcgcc gtctgcggcc gcttatgaaa tattggaact tttaacgttg gaattgtaaa
      901 atccccgttt cgggcaagcc ttgacggatt tgccgatatg ctgtccggca cacaagccgc
      961 atcaaattat tttgatttta ttttaacaaa gaatgcccct gatggggcaa gctattctta
     1021 ttcagaccaa ggaccagtat gttagacaat ttaaccggcc gcttcagcaa tgtcttcaaa
     1081 aacatccggg ggcaggccaa actgaccgaa gacaatatta aagaggcctt gcgcgaagtc
     1141 cgcctcgccc tgcttgaggc ggatgtcgcc ctgcctgtcg tcaaagagtt cgtcaacaac
     1201 gtcaaagaaa aggccctcgg tcaggaagtg gctggcagcc tgacgccgga tcaggcattt
     1261 atcggcgtgg tcaacaaagc cctgaccgaa ctgatgggca gggaaaacaa aacgctggat
     1321 ttgtcggttg cgccgcccgc cgtcgtgttg atggcaggtt tgcagggcgc gggcaagacg
     1381 acgaccgccg gcaaactcgc ccgcctgctg aaaaacgatc agaagaaaaa ggttttggtc
     1441 gtatctgccg acgtttaccg ccctgccgcg attgaacaac tgcgcctgtt ggccgaacag
     1501 gtcggcgtgg actttttccc atccgatacc aaccaaaaac cggttgaaat tgcaactgcc
     1561 gccgtcgatt acgccaaaaa acatttttac gatgttttga tggtcgatac cgccggccgt
     1621 ctggcaatcg atgaagagat gatgaacgaa atcaaagccc ttcacgcggc ggttaacccg
     1681 gtggaaactt tgttcgtcat cgatgcgatg ctgggtcagg atgcggtgaa cactgctcag
     1741 gcatttaatg aagccctgcc gctgaccgga gtcgtattga ccaagatgga cggcgactcg
     1801 cgcggcggtg cggcattgtc cgtacgccat gtaaccggca aaccgattaa atttatcggt
     1861 gtcggcgaaa aaatcaacgg cctcgaacct ttccaccccg accgtcttgc cagccgtatc
     1921 ctcggcatgg gcgacgtatt gaccctgatt gaagacgttc aaaaaggtat tgatgaagaa
     1981 gccgccgcca aaatggcgaa aaagctgcaa aaaggcaaag gcttcgacct caacgacttt
     2041 aaagaacaaa tccagcaaat gcgcaatatg ggcggtttgg aaaacctgat gtcgaaaatg
     2101 ccgggcgaac tgggtcaaat atcgaaacaa atccccgaag gtacggctga aaaagcgatg
     2161 ggtaaagtag aagccatcat caactcgatg acccctaaag aacgcgccaa ccctgccctg
     2221 ctcaaagcca gccgcaaacg ccgtattgca atgggtgcgg gcacgaccgt acaggaagtg
     2281 aacaaattgc tcaaacagtt tgaacaaatg caacaaatga tgaagatgtt cagcggcaac
     2341 ggcttgggca aactgatgcg tatggcgaaa ggaatgaagg gaatgaaagg aatgttcccg
     2401 ggtttgtaag ccgatttgac cgaaaacgcc gtctaaaatt tcagacggcg tttttgtttt
     2461 atattctgat ttatagtgga ttaacaaaaa tcaggacaag gcgg
//
LOCUS       DAWFAD010000198         2499 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWFAD010000198 DAWFAD010000000
VERSION     DAWFAD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.198"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            224..1432
                     /locus_tag="WGO49_001924"
     CDS             224..1432
                     /locus_tag="WGO49_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0306978.1"
                     /translation="MSEAEYFSHFISNGNGKLLEIPQRRGRQDGVFIDWLSFTLHEDS
                     LLKVSGCPLVSDAEYMFVLSKKLEEILGFGITSRCKSKGNKFYDSMFRLGSEEVDYGE
                     VHYGGQRNTVLIELKGVGCNIANPGWELRLKQFLEDSLRPRITRVDLALDFFDGEYTP
                     EQALLDHDNGFFDNSNMRPKSEMVGTAWRREDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNHGDMEIPLDILINQGSYFSGAFPICQKFKNMPNPERFDYRKKV
                     ANLTFQHKLRYAKNAVGKLINFMFDMGFDSDEIVRYLKADLGYPKGLEPEKYSLAGLK
                     ESLKFGFIHEQPDVDLEVELEELGIIKFKQSDKFDPDKRLFDPHHDVESERQYQLYLD
                     RMYDLHANQN"
     gene            1453..1764
                     /locus_tag="WGO49_001925"
     CDS             1453..1764
                     /locus_tag="WGO49_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306979.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSKLENLKYPCEVMVTVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            1768..1968
                     /locus_tag="WGO49_001926"
     CDS             1768..1968
                     /locus_tag="WGO49_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306980.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEIGGGFQIFQFFVKSE"
     gene            2195..2425
                     /locus_tag="WGO49_001927"
     CDS             2195..2425
                     /locus_tag="WGO49_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306981.1"
                     /translation="MNIVKKYAVKAALAAGIFTPAIVMADTFDASAIGTQVANVIMGF
                     VSMVSAVGMAAITVILAIQGFKMAWSMIKSVR"
BASE COUNT          779 a          423 c          595 g          702 t
ORIGIN      
        1 gcgttcgatt atatagattc ggttggctcg aattttccgg taattatcac aacagacggt
       61 tgtggtcttt cttcttgatc tttaacagtt tgtcaggatt gggctttcgg tcgttgaccg
      121 ttggacgcgc tttagcgcgg cagacgggaa acggctgaaa gccccccccc tgactaacag
      181 ggggggagcg aaataaaaca aatccctaaa ggtactgaac aaaatgagtg aagcagaata
      241 tttttcccat ttcatatcta acggcaatgg gaagttatta gaaattccgc aacgtagggg
      301 caggcaggac ggcgttttta tagattggtt gtcattcaca ctgcacgaag attccttgct
      361 gaaagtttcc ggatgcccct tagtttccga tgccgaatat atgtttgttt taagcaaaaa
      421 attggaggaa atattagggt ttggcatcac gagcagatgc aaatcgaagg gcaataaatt
      481 ttacgattcg atgtttaggt tgggatcgga agaagttgac tacggcgaag tccattacgg
      541 aggtcagcga aatacggttt taatcgaatt gaaaggtgta ggttgcaaca ttgcaaatcc
      601 aggttgggaa ttgaggctta agcagttttt ggaagattca ttgaggccga ggataacgcg
      661 ggtagatttg gcacttgatt tttttgatgg ggagtacacg ccggaacagg cacttttgga
      721 tcacgataac ggttttttcg ataacagtaa catgaggccg aaatctgaaa tggttggaac
      781 ggcttggcgg agagaggacg ggagcggcaa gacattttat gtaggtcgca agaaaaattc
      841 tcgttttgtg cgtgtttatg agaaaggcag gcagctaggc gataaagaaa gcaagtgggt
      901 aaggttcgaa attcagttta atcatggaga tatggaaata cctttggata ttctgataaa
      961 tcaaggttct tacttttcag gcgctttccc gatttgtcag aaatttaaaa atatgccgaa
     1021 tccggaaagg ttcgattacc gtaaaaaagt ggctaattta acttttcagc ataaattgag
     1081 atacgcaaaa aacgcggtcg gcaaactgat taatttcatg tttgatatgg gttttgatag
     1141 tgatgaaatt gtcagatatc tgaaggcaga tttggggtat cccaaagggc tagaacctga
     1201 aaaatattcg ttggccggat tgaaggaatc tttgaaattc ggctttatcc acgaacaacc
     1261 ggatgtagat ttagaggttg aattggaaga actcggaatt atcaaattta agcaatcaga
     1321 taaattcgat ccggataaaa ggcttttcga tccacatcac gatgtagaaa gtgagaggca
     1381 atatcagctt tatctcgaca gaatgtatga tcttcatgca aatcaaaatt aacctaaaaa
     1441 ggaaaaatta atatgtttaa tcaaactcaa actgtaactt atcccgcaac ttttttagga
     1501 gctaaaaaat tcaaaggcga aattgatggc tctaatatcg acacttgttc cgtattggtt
     1561 gcaacacctt tgccggcaca gtcgggaaat gctgttggat tcacggcagc acaaatgaag
     1621 ttcggggaca gtaagaattt ctcaaaatta gagaatctca aatacccgtg cgaagttatg
     1681 gtaacggttg aaatgacttc gacaggtaag ggcatggttc cttcattaat tgattttcag
     1741 gtggcagaaa agccgaaagg ttgatttatg aaatttgaag aacgtttcat agttcaagac
     1801 ttggaaacgc atgactttat ttatcccgat cctttcggtg atgtggggtt tactcaaaat
     1861 attaaatcag caggtcaatt tgaaagctac gaagatgcgt tgaattcagg cataaatgaa
     1921 ataggcggag gattccagat atttcagttc ttcgtaaaat cggaataaaa gaaaaacagg
     1981 ctcggcgggc ggtctgtcaa cctttcacaa agcccgcaaa aaggattaaa aatgaataaa
     2041 gcaactttaa ttatcggatt ttttgtttgc ctcattcttt tcattttctc aattctctat
     2101 ttcataaatt aaaaacaaaa ttcgggagca ggcttcgccc ggatttaaac ctaagaagcc
     2161 gtattacttt aaatatcaaa aaaggaaaaa aacgatgaac atcgttaaaa aatacgctgt
     2221 aaaagcagcc ttggcagccg gtatcttcac accggccatt gttatggcag atacctttga
     2281 tgcatccgcg attggtacgc aagtagcgaa tgtaatcatg ggtttcgtgt caatggtttc
     2341 cgccgtgggt atggcggcca ttaccgtgat tcttgcaatc caaggcttca aaatggcttg
     2401 gagcatgatt aaatctgtca gataaacaga gtgaagaaaa aggggcgtat aaatgggcta
     2461 tcgtgtcggc ataaattgtt ttgatacaag attgcaggc
//
LOCUS       DAWFAD010000199         2440 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWFAD010000199 DAWFAD010000000
VERSION     DAWFAD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.199"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(425..2305)
                     /locus_tag="WGO49_001928"
     CDS             complement(425..2305)
                     /locus_tag="WGO49_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HEZ0306982.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFSAEAIAEEYFGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKISAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAVKPGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAHRPAVSIYFDVDSE
                     FNTGEPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAIAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSIVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENDLPGLFRVQPAGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLFQQSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          497 a          589 c          722 g          632 t
ORIGIN      
        1 atttccacct gcgcgggaat gacgattcag aagttgcccg aaacctaaaa aactgaaacc
       61 gaacgagccg gatttccgct ttcgcgggaa tgacggcgta taagttcccg tgcggacaga
      121 cctatattcc cacctgcgcg ggaatgacga ttcagaagtt acacgaaact caaaaaaaac
      181 gaaaccgaac gaaccggatt cccgctttta cgggaatgac ggcgcataag ttcccgtgcg
      241 gacagaccta gattcccacc tgcgtgggaa tgacgggatt ttagattgcg ggtatttatc
      301 gggaacggcg gcttggaagt tcattgaaac ggaaaaacaa cggaaaccca aaaaaccgga
      361 ttcccgactg tgggaatgat gagattcagg tttctgtttt tgccggagtt tgccgtatcg
      421 ggcttcagac ggcattgcct gccgttgtac ccgcgggtgc gactgccttg atgtagttga
      481 gcgagacaaa ctgcttctcg gcatccaatt cggtgatttt gaacaatgcc tgtgatttgg
      541 gcagcgcgtc aaacggaata ccggtcgcgc gcgttaccag cggcaggcct tcaatgcgga
      601 cgaggtcttc tttgaggatg gtggccgtca gctcgctcgt gccttgctgt tgcagataga
      661 cgaggctcca gtaggcttcc atttggcgtt ggaagtcggc gtaggctgta taggcggcat
      721 caaagtcgcg cagcgcggcg aagagttcgg catcgctttg ttggaaaagc ggctcggcgg
      781 tgtcgtctat caggctgatc agttgctttt ggttgatgta gtcggcggcg cggcgcagcg
      841 gcgaggtaaa ccagccgtaa tgctgcacgc ccattccgat gtgcggctcg gatttggtgc
      901 tcatacgcac tttgcctgcc ggttgcacgc ggaaaaggcc gggcaggtcg ttttcatcca
      961 acatttgcgc ccaagtgctg ttggcaagaa tcatcatctc gctgaccagc gtatcgatgg
     1021 gagaaccgcg ttcgcggcgg acgatggata cgttgccttc ctcatccaat tcgatgctgt
     1081 aatcgtattg cggcgcacgg tcgggctcgt atttgccgcg tgctttttgc agggcgatgg
     1141 cgaattgata gaaccaaatc aggtcttgat ggtgggcgaa catcatttcg cccgcttcgt
     1201 ccaagcccgc ttcggcgttg aaatgcggtt cgatggcttg gatacgcagg ttttcggaga
     1261 tgttgaccgc ttcgattttg caggtcggtt cgccggtgtt gaactcgctg tccacatcga
     1321 aataaatgct gacggcaggg cggtgtgcgc ctgcatcaag gctgaacgcg gcaatccagt
     1381 tttcgggcag catcgtgatt ttgccgccgg ggaaataaac cgtgctcaag cgttccatga
     1441 tgtttttttc cattttgtcg cccggtttaa cggcaagtga cggcgcggcg atgtggatgc
     1501 cgacacgctt cgtgccgttg tccaagtcgg tcaggcttaa agcgtcgtcc acttcggtgg
     1561 tcgattcgtc gtcaatggaa aaggcggtaa cgtcggcctt gggcaggtcg ggcatttcgg
     1621 gaagggcaag gtcggggaag cctgtgcctt taggaaagta tttgatttca aacccgtctt
     1681 gcaggtattg cggaatggac gtaatgccac ccgttttttt cgccaattcg taggcggaga
     1741 ttttcagtgc gtcggcggct ttggtaaagg ctttgtaggt cagcgactgt ttgtcgggcg
     1801 cgtgcaggat ggttttcaaa tccgccgcga tttcagacgg catctcgccc cgttttaagg
     1861 cttccgccca agcgtcgatt tgcgcgtctt gctgtttttt gcgttcgatg gcggcaagcg
     1921 cttgtttcaa agtttcttcg ggcgcggctt tgaacacgcc tttggctttt ttgtagaaat
     1981 acatcggcgc ggcgtaaagc gcaatcaagg tcgccgccag ctcggttttg gtcggcgcat
     2041 gaccgaaata ttcttcggcg atggcttcgg cggaaaattc ttcttcgccg catacttccc
     2101 acaataaatc ggtgtcgatg tccgccgcct gtgcctgcgc gttttccaaa aacgccgcca
     2161 tatcgccgtc aaactcggca aagacattgt ttgccttcac tttggtgcgc ttgccgtgtt
     2221 gggtatcgac ttggtaggta gcgtcgtttt tttggatgat ggcggcgatt ttgaattggc
     2281 cggactcttc gtaaaaaata ttcatttttc ggatttttct gtggaaactc aagcgggcga
     2341 ttttagcaga ttaccgaaaa tgccgtctga aaaaaggttg ggagagggtt ggcgcgtctt
     2401 tgcggtgctt gcgttatagt ggattaacaa aaaccagtac
//
LOCUS       DAWFAD010000200         2434 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWFAD010000200 DAWFAD010000000
VERSION     DAWFAD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.200"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..332
                     /locus_tag="WGO49_001929"
     CDS             <1..332
                     /locus_tag="WGO49_001929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306983.1"
                     /translation="NPHHRHSRAGGNPKTQRNRNLSEKTETPPTVIPAKAGIQQHNAA
                     GIYRKKQKPPPTVIPAKAGIQQPKNHRNLSAKTETPTAVIPAKAGIQTRRHGNLPNKT
                     VSLDSTS"
     gene            477..2246
                     /locus_tag="WGO49_001930"
     CDS             477..2246
                     /locus_tag="WGO49_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ0306984.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQANATDTDEDEELESVVRSALVLQFMIDKEGNGEIESTGDIGWSIYYDDHNTLHG
                     ATVTLKAGDNLKIKQSGKDFTYSLKKELKDLTSVETEKLSFGANGNKVNITSDTKGLN
                     FAKETAGTNGDPTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLNAGW
                     NIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGAKTS
                     VIKEKDGKLVTGKGKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKTTTANGQTGQAD
                     KFETVTSGTKVTFASGNGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGWNLDSK
                     AVAGSSGKVISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMTPQFSSVSLG
                     AGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQLKGVAQNLNNRI
                     DNVDGNARAGIAQAIATAGLAQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDTGNWV
                     IKGTASGNSRGHFGTSASVGYQW"
BASE COUNT          747 a          615 c          591 g          481 t
ORIGIN      
        1 gaaaccctca ccaccgtcat tcccgcgcag gcgggaatcc aaaaacgcaa cgcaacagga
       61 atttatcaga aaaaacagaa accccaccga ccgtcattcc cgcaaaagcg ggaatccagc
      121 aacacaacgc agcaggaatt tatcggaaaa aacagaaacc cccaccgacc gtcattcccg
      181 caaaagcggg aatccagcaa ccgaaaaacc acaggaatct atcagcaaaa acagaaaccc
      241 ccaccgccgt cattcccgca aaagcgggaa tccagacccg tcggcacgga aacttaccga
      301 ataaaacagt ttccttagat tccacgtcct agattcccgc cttcgcggga atgacgagat
      361 tttaagttgg gggaatttat cagaaaaccc ccaaccccca aaaaccgggc ggatgccgca
      421 ccatccgccc ccaaaccccg atttaaccat tcaaacaaac caaaagaaaa aacaaaatga
      481 acaaaatata ccgcatcatt tggaatagtg ccctcaatgc ctgggtagtc gtatccgagc
      541 tcacacgcaa ccacaccaaa cgcgcctccg caaccgtggc gaccgccgta ttggcgacac
      601 tgctgtccgc aacggttcag gcgaatgcta ccgataccga tgaagatgaa gagttagaat
      661 ccgtagtacg ctctgctctg gtgttgcaat tcatgatcga taaagaaggc aatggagaaa
      721 tcgaatctac aggagatata ggttggagta tatattacga cgatcacaac actctacacg
      781 gcgcaaccgt taccctcaaa gccggcgaca acctgaaaat caaacaaagc ggcaaagact
      841 tcacctactc gctgaaaaaa gagctgaaag acctgaccag tgttgaaact gaaaaattat
      901 cgtttggcgc aaacggtaat aaagtcaaca tcacaagcga caccaaaggc ttgaattttg
      961 cgaaagaaac ggctgggacg aacggcgacc ccacggttca tctgaacggt atcggttcga
     1021 ctttgaccga tacgcttgcg ggttcttctg cttctcacgt tgatgcgggt aaccaaagta
     1081 cacattacac tcgtgcagca agtattaagg atgtgttgaa tgcgggttgg aatattaagg
     1141 gtgttaaaac tggctcaaca actggtcaat cagaaaatgt cgatttcgtc cgcacttacg
     1201 acacagtcga gttcttgagc gcagatacga aaacaacgac tgttaatgtg gaaagcaaag
     1261 acaacggcaa gagaaccgaa gttaaaatcg gtgcgaagac ttctgttatt aaagaaaaag
     1321 acggtaagtt ggttactggt aaaggcaaag gcgagaatgg ttcttctaca gacgaaggcg
     1381 aaggcttagt gactgcaaaa gaagtgattg atgcagtaaa caaggctggt tggagaatga
     1441 aaacaacaac cgctaatggt caaacaggtc aagctgacaa gtttgaaacc gttacatcag
     1501 gcacaaaagt aacctttgct agtggtaatg gtacaactgc gactgtaagt aaagatgatc
     1561 aaggcaacat cactgttaag tatgatgtaa atgtcggcga tgccctaaac gtcaatcagc
     1621 tgcaaaacag cggttggaat ttggattcca aagcggttgc aggttcttcg ggcaaagtca
     1681 tcagcggcaa tgtttcgccg agcaagggaa agatggatga aaccgtcaac attaatgccg
     1741 gcaacaacat cgagattacc cgcaacggca aaaatatcga catcgccact tcgatgaccc
     1801 cgcaattttc cagcgtttcg ctcggcgcgg gggcggatgc gcccacttta agcgtggatg
     1861 acgagggcgc gttgaatgtc ggcagcaagg atgccaacaa acccgtccgc attaccaatg
     1921 tcgccccggg cgttaaagag ggggatgtta caaacgtcgc acaacttaaa ggtgtggcgc
     1981 aaaacttgaa caaccgcatc gacaatgtgg acggcaacgc gcgcgcgggt atcgcccaag
     2041 cgattgcaac cgcaggtttg gctcaggcct atttgcccgg caagagtatg atggcgatcg
     2101 gcggcggtac ttatcgcggc gaagccggtt acgccatcgg ctactcgagc atttctgaca
     2161 ctgggaattg ggttatcaag ggcacggctt ccggcaattc gcgcggtcat ttcggtactt
     2221 ccgcatctgt cggttatcag tggtaagggc tttttcgcct gtctgctgtt ggggcaggcg
     2281 gaaggtttga agggaagggt ggcgatttgc cgcctgagac ctttgcaaaa ttccccaaaa
     2341 tcccctaaat tcccaccaag acatttaggg gattttccat gagcaccttc ttccagcaaa
     2401 ccgcccaagc catgattgcc aaacacatca accg
//
LOCUS       DAWFAD010000201         2413 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWFAD010000201 DAWFAD010000000
VERSION     DAWFAD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.201"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(4..624)
                     /locus_tag="WGO49_001931"
     CDS             complement(4..624)
                     /locus_tag="WGO49_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0306985.1"
                     /translation="MTTHHVELRNVTKRFGAQKAVNQVDLVLKAGESVGLAGYNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGARLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            complement(682..1914)
                     /locus_tag="WGO49_001932"
                     /pseudo
     CDS             complement(682..1914)
                     /locus_tag="WGO49_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            complement(1917..2342)
                     /locus_tag="WGO49_001933"
                     /pseudo
     CDS             complement(1917..2342)
                     /locus_tag="WGO49_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
BASE COUNT          521 a          626 c          661 g          605 t
ORIGIN      
        1 aatttaatcc acgataatgc ggtcggcgtg tccgtccaac tcggcaaggg cgtgggtgct
       61 gagcaatacg gtcgcgccgc gcccgttgag ttcgcgcacg acttcgtaaa acatttgtcg
      121 tgatgcaggg tcgaggccgg ttgtcggttc gtcaaacagc aggactttgg gctcgcccag
      181 cagggcttgt gccaaggcaa ggcgttggcg catcccttta gaataagtgc cgacgcggcg
      241 gtgtgccgcc tgtgaaatgc cgacgcgctc aagcagcccc cggttctgcg tgagcggctg
      301 ttttttaagt ttggcataaa aatccagcgt ttcgatgccg gtcagcgaag ggtgcagcgc
      361 aacggtttcg ggcaggtagc cgatttggct gcgaagccgc gcccccgctt tgctgccggt
      421 acgttcgccc aaaagcatca cttcgccttc ggtcggggta atcagcccga gtatcagctt
      481 cataatggtg gacttgcccg cgccgttgta tccggcaagc ccgacgcttt ctcctgcctt
      541 caaaaccaaa tcgacttggt tgacggcttt ttgcgccccg aaccgtttgg tgacattcct
      601 caattcgaca tgatgtgtgg tcatggttgt tccttttgtg ttttcagacg gcatttggat
      661 ccggatgccg tctgaagcag actagttcaa agaaccgttt tccgccctgc cccattccga
      721 ctgccgtgtt tcggcttctt tgagcaactc gtccttcatt gcctgatagc gggtttgaat
      781 tttggggaca taaggcttca tcagcggttt gctgtccacc acgccgccgg gcagaacggc
      841 gggaaactgc gcctgcgccc atttgacgat gctgattgcg agactgttca tcaagaggcg
      901 caatacgggc gcgcgccaga tgatttggtc gatgatgccg ttgggccggt acgcgctgtc
      961 gccgaagccg tcgccgttca aatcgaacgc actgttgtcg ctccaatagt tgccgtgtcc
     1021 gccctcgctc cagtcgagaa agcgcgtgct gacgtatttg acctggcttt cgttgttgat
     1081 aaaggaattg tcgtgcaggg acgtgccttc gatggcggcg gtaaagtgta tgccgatttg
     1141 gcagttttca aaatgattgg cggacagttt atcgtagttg gcattgtagg caaaaacgca
     1201 cttgcccgct ttgttgataa tgttgtcgtg aatatcggaa tagttgacat agttgagcat
     1261 gatgccttgg tcgcggctgc cgacggcgat attgtcaaac actttgagcc gctcggaaaa
     1321 catcagcaca tagcccatat tgttgcccac ggaaatattg ccgctgattt cgctgtcgtt
     1381 ggtgtacata tagtggacgg cgaaacgcaa atcgctgaag cggttgtttt tataggtgtt
     1441 gtgcgtgctg gtattggaaa aaatgccgtc gcggcctttg gaaatatcgt tgccgacgac
     1501 ctgcgcgccg ggcgcgttcc aaacggtaac gccgttgcct cgctcgttca cgcgcaaagt
     1561 cgcatcgcca acaattttgt tctcgcgcac catcgcatcg gcagaaccat gcagatatac
     1621 gccgaccgaa ttatccaaaa tattgttgtg ttcaatcagg gcgcgcgggg cggtttcttc
     1681 gagataaata ccggcatcca ttgcaggcag gctcataccg gaacgggtaa cggtcaggtt
     1741 gcggagcgtt acgtccggcg catgcacagc tatcgtacgt ccgctcctgt ctccttcgat
     1801 ggttgcagaa cggtcagcag gcccttcaat cgtaatcggt ttgtcgatat aaagtttggt
     1861 ttgatatacg ccggaagcca gtttgagcgt atcgcccgca tgggcgcggg caaatgcggg
     1921 ataccgatca gcataatcgg ttcgtgatga tcgaccagtt tcgctccggc tatcgtgccg
     1981 tcatttgcca aagccatcag cgtatcgatc ggtttgttcg aataaccgcg cgtattgacc
     2041 gcatcggtcg tgatatagac caagcccaac tgctcatcgc ctttgtaaac gcgggcaacc
     2101 ataggcttgc cttccggctt gccgtaacgg tccgcacccg gaaaaatttc cgaaggctgt
     2161 attttcgcca gaaaatcagg cagacgctcc gcataagcgg gcagtgccgc cgtcaaaaag
     2221 acaaaggcgg taaaaaacac tgttatccgc gcccgaaacg cggacatacg tttgattgaa
     2281 aaacaggaca tgactatccc cgatataccg atactcttta atattgatat tatatgaatc
     2341 atctatcggc gattttttga tgcaaattaa atttacaaat cttaaaacca atccgcatac
     2401 gggataatat agt
//
LOCUS       DAWFAD010000202         2380 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWFAD010000202 DAWFAD010000000
VERSION     DAWFAD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.202"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            27..572
                     /locus_tag="WGO49_001934"
     CDS             27..572
                     /locus_tag="WGO49_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0306986.1"
                     /translation="MGKTVTLTSGHSNTDPGAVNGSDREADLAQDMCNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGQHSRLAYAQAGGIVFEPFFISNDTDLALFKTT
                     KWGICRAIADAIAMELGAARV"
     gene            569..775
                     /locus_tag="WGO49_001935"
     CDS             569..775
                     /locus_tag="WGO49_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306987.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            778..942
                     /locus_tag="WGO49_001936"
     CDS             778..942
                     /locus_tag="WGO49_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306988.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            945..1181
                     /locus_tag="WGO49_001937"
     CDS             945..1181
                     /locus_tag="WGO49_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306989.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTVKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETAGE"
     gene            1153..1572
                     /locus_tag="WGO49_001938"
     CDS             1153..1572
                     /locus_tag="WGO49_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306990.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTLLAVSHHQGYKSAFAKQQAVID
                     KMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
     gene            1469..1774
                     /locus_tag="WGO49_001939"
     CDS             1469..1774
                     /locus_tag="WGO49_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306991.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(1819..1989)
                     /locus_tag="WGO49_001940"
     CDS             complement(1819..1989)
                     /locus_tag="WGO49_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ0306992.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            2002..2220
                     /locus_tag="WGO49_001941"
     CDS             2002..2220
                     /locus_tag="WGO49_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306993.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            2217..>2380
                     /locus_tag="WGO49_001942"
     CDS             2217..>2380
                     /locus_tag="WGO49_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306994.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDF"
BASE COUNT          617 a          544 c          687 g          532 t
ORIGIN      
        1 caatcaatat tttttggaga tgatgaatgg gcaaaaccgt aaccttaacc tctggacaca
       61 gcaacaccga cccgggtgcg gtcaacggca gcgaccgtga ggcggacttg gcgcaggata
      121 tgtgcaatat cgtggctgct attttgcgcg atgactacgg tttgactgtt aaaaccgacg
      181 gcacgggcaa aggcaatatg ccgctgcgtg aagcggtcaa gctgattcgc ggctcggatg
      241 tggcgattga gtttcacacc aacgctgccg tcagcaaagc ggcgacaggc atcgaagcct
      301 tgagtaccgt taaaaacaaa cgctggtgtc aggtgttgag caaagccgtt gccaagaaaa
      361 ccggctggaa actgcgcggc gaagacggct ttaaacccga caatgcgggc cagcattcgc
      421 gcctggctta tgcacaagcc ggcggcattg tgtttgagcc ttttttcatc agcaacgaca
      481 ctgatttggc cttgtttaag acgactaaat ggggcatctg ccgcgcgatt gcggacgcga
      541 ttgcgatgga attgggggcg gcaagagtat gaatattatt ggtaaattga aagaagctgc
      601 ttcctatttt cttacaaaat tgattggaga aaatcctagt aatgagcagg taaaccgcgc
      661 acttatacag atgccaaatg ttcgtccgat acacacctat ccacgcccaa atttaagaaa
      721 ctcaggcgtg gcagccgcga aacgcgcggc gcgcaaacgc aagaatcgtc gttaatcatg
      781 ggacaggttg cgttttacga aaagatgatt gggctgtggt cggccaaaag ccgtgaggca
      841 agcgaacagg cagacttggc ggcgtttgaa tttgcggagg gcgaactggc caattatcag
      901 gaaatgctga aacggcacct gcaaaccaaa agtgtggaat agcaatgcgt attttggata
      961 tttttaaaaa cccagcgaca ggcaatgtgt cgcactcgaa actgtgggca aacgttgcct
     1021 gcgcggcggg gacggttaag tttgtgatgc tgcccgaccc gtcggcggag atttgggcgg
     1081 tgtatttggg cattgtcggc ggctatgcgg tggcgcgttc gttggtcagc gtcaaacgtc
     1141 aggaggtcga gaatgaatct cgtgaaactg ctggcgaata actggcaacc gattgccatc
     1201 atcgcgcttg tcggcacgct cttggcggtg tcgcaccatc aaggctacaa gtcggctttt
     1261 gcgaagcagc aggcggtcat tgacaagatg gaaaaagaca aagcgcaagc cctgctgttg
     1321 tcggctcaaa actacgcccg cgagctggaa caggcgcgtg cggaagctaa aaaatatgaa
     1381 gtcaaggcgc acgccgtcgg catggctttg gcgaaaaaac aggcggaagt cagccgtctg
     1441 aaaacggaaa ataaaaagga aatcgaaaat gtccttactc aagaccgtaa aaatgcaggc
     1501 ggcggttgta ttgacggctt tggccatcac ggcttgcagc tctacaagcg cgccctcggc
     1561 tacggaaatt aaggttgtcg aaaaggcggt catgccgaca ccgcctgccg cattgatggt
     1621 cgcgccggtg cgcccgaatc cgccgaaaga cggcaagacg gccacgctgt tggaacacgc
     1681 cgccgagttt ggcggctatg ttgccgaact ggaaaatcaa aatcaggctt ggcgcgactg
     1741 ggcgggcaat cactcccgca aagtcggaaa ctgacaaaaa agcccgcgta gggcgcgggc
     1801 tgagggtgaa agcggatttt atacctcttt tacaggggta gcggcggtag tgcttttcag
     1861 caaatcgact gcgtgctgac agttttgctt gctggtgtag ccttcgccct gagcgatgat
     1921 ttcatggttg gctgctttca aacgccaacg gtattcgcct tttgcgtctt tatagatttc
     1981 aaaatacata aggtttctcc tatgaatgag tacacgtttt cttaccgctt taacggcaag
     2041 tcctggtcat tgagcatttg ggcggacaac cctgaagaag ccagggcgaa atttcgggct
     2101 gcacgagaaa atgcgcacta tgacggcgaa gttgtagcaa aggtttatac atttgtaaat
     2161 atttcgtggg ttaagaaatt gtacaagcgg acaaaatatt taatgggtat caaagaatga
     2221 cctaccgtga attagttgaa cgtcagttgg ctgtgcgcca tgccgatttg gaattgggct
     2281 taagccgcgc acgcgaacaa gagccgtttg tcattcatgt ttccgatctg ttggataagg
     2341 caggcattga gtacgcggta cgcatggata aggattttca
//
LOCUS       DAWFAD010000203         2338 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWFAD010000203 DAWFAD010000000
VERSION     DAWFAD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.203"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            43..2256
                     /locus_tag="WGO49_001943"
     CDS             43..2256
                     /locus_tag="WGO49_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0306995.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNVPDNPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGS
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPHVLASLGDVKGVLSVTRL"
BASE COUNT          599 a          755 c          564 g          420 t
ORIGIN      
        1 ctgttacaat aaactcgttt ttatcggaac gaaagaccca tcatgaccgc catcagcccg
       61 attcaagaca cgcaaagcgc gactctgcaa gaattgcgcg aatggttcga cagctactgc
      121 gccgcgctgc cggacaacga taaaaaactt gtcttagccg cccgttcgct ggcggaagca
      181 cattaccccg ccgatgccgt cacgccgtat ggcgagccgt tgtccgacca cttcctcggc
      241 gcggcacaaa tggtcgacga actcgacctg ctgcccgatg ccgtcgccgc caccctgctt
      301 gccgacatcg gacgctacgt ccccgattgg aatctattgg tttccgaacg ctgcaacagc
      361 accgtcgccg agctggtcaa aggtgtggac gaagtgcaga aactcaccca cttcgcccgg
      421 gtggacagcc tcgccacgcc agaagaacgc gcccagcagg cagaaactat gcggaaaatg
      481 ctgctggcga tggttaccga catccgcgtc gtgttaatca aactggcgat gcgtacgcgc
      541 accctgcaat tcttaagcaa cgttcccgac aatcctgaaa agcgcgccgt tgctaaagaa
      601 accctcgaca tttttgcccc actcgccaac cgcttgggcg tgtggcaact caaatggcag
      661 ctcgaagatt tgggcttccg tcatcaagag ccggaaaaat accgcgaaat cgccctgctt
      721 ttggacgaaa aacgcaccga gcgcctcgaa tacatcgaaa acttcctcaa tatcctgcgt
      781 acggaactca aaaaatacaa tatccacttt gaagtcgccg gccgcccgaa acacatctac
      841 tccatttaca aaaaaatggt gaagaaaaaa ctcagcttcg acggcctctt tgacatccgc
      901 gccgtgcgga ttctggttga taccgtcccc gagtgttaca ccacgctggg cattgtccac
      961 agcctttggc agcctatccc cggcgagttc gacgactaca tcgccaaccc gaaaggcaac
     1021 ggctataaaa gtttgcacac cgtcatcgtc ggtcccgaag acaaaggtgt ggaagtgcaa
     1081 atccgcacct tcgatatgca ccaattcaac gaattcggtg tcgccgccca ctggcgttac
     1141 aaagaaggcg gcaaaggcga ttccgcctac gagcaaaaaa tcgcctggtt gcgccaactt
     1201 ttggactggc gcgaaaatat ggcggaaagc ggcaaggaag acctcgccgc cgccttcaaa
     1261 accgagcttt tcaacgacac gatttatgtc ctgaccccgc acggcaaagt cctctccctg
     1321 cccacgggcg cgacccccat cgacttcgcc tacgccctgc acagcagcat cggcgaccgt
     1381 tgccgcggtt cgaaagtcga agggcagatt gtgccgctgt ccaccccgct cgaaaacgga
     1441 cagcgcgtcg aaatcattac cgccaaagaa gggcatcctt ccgtcaactg gctttacgaa
     1501 ggctgggtca aatccaacaa ggcaatcggc aaaatccgcg cctacatccg ccagcaaaac
     1561 gccgacaccg tgcgcgaaga aggccgcgtc caactcgaca aacagcttgc caaactcacg
     1621 cccaaaccca acctgcaaga gcttgccgaa aatctcggct acaaaaagcc agaagacctc
     1681 tacaccgccg tcggacaagg cgaaatttcc aaccgcgcca tccaaaaagc ctgcggcacg
     1741 ctgaacgaac cgccgcccgt acccgtcagc gaaaccacca tcgtcaaaca gtccaaaatc
     1801 aaaaaaggcg gcaaaaacgg cgtgctcatc gacggcgaag acggtctgat gaccacgctt
     1861 gccaaatgct gcaaacccgc gccgcccgac gacattgtcg gcttcgttac ccgcgatcgc
     1921 ggcatttcgg tacaccgcaa aacctgcccc tctttccgac acctcgccga acacgcgccc
     1981 gaaaaagtac tggacgcaag ttgggcggcg ttgcaggaag gacaagtgtt cgccgtcgat
     2041 atcgaaatcc gcgcccaaga ccgctccggg cttttgcgcg acgtatccga cgcgctcgcc
     2101 cgccacaaac tcaacgttac cgccgtgcaa acccagtccc gcgacttgga agccagtatg
     2161 cggttcacgc tcgaagtcaa acaggtcaac gacctcccgc acgtcctcgc cagcctcggc
     2221 gacgtcaaag gcgtattgag cgttacccgg ctttaaatac aaaaatgccg tctgaaagcc
     2281 gtataacgct tcagacggca ttttgattga cggggtttgc tattttttgt tgcatagt
//
LOCUS       DAWFAD010000204         2335 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWFAD010000204 DAWFAD010000000
VERSION     DAWFAD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.204"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            31..1248
                     /locus_tag="WGO49_001944"
     CDS             31..1248
                     /locus_tag="WGO49_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0306996.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTAIAIIGLANIAGCVF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLKMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            complement(1451..1591)
                     /locus_tag="WGO49_001945"
     CDS             complement(1451..1591)
                     /locus_tag="WGO49_001945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0306997.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1604..>2335
                     /locus_tag="WGO49_001946"
     CDS             1604..>2335
                     /locus_tag="WGO49_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0306998.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYRIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKS"
BASE COUNT          547 a          724 c          539 g          525 t
ORIGIN      
        1 cgcaaactta accgactcaa aatacacatt atgacgcaca ccgcatcgaa aacacccaaa
       61 ttttggctga ccatcaccgc cgccgcattc atcctgctga ttaccatcgg catgaggatg
      121 acgctcggac tgttcgtcca acccgtcgtc aacaccaccg aattgagcat tgcccaattc
      181 agcctcatca tcgccgtttt ccaactgatg tggggcatat cgcaaccatt gtccggcgcg
      241 cttgccgacc gtttcggcgc gttcagggta ttaagcggcg gcgccgtcct gctcgtctgc
      301 gcctgcctga ttgcccccaa catcccgact tattgggggc tgatgattgc cgtcgggctg
      361 ctgctcgcat tcggcaccgg ttccggcggt ttctccatca ttatggggca ggtcgccgcc
      421 caagtccccg cacacaaacg cggcttggca tccggactgg tcaacgccgg cggttcggca
      481 ggacaattcc tgttcgcacc gctggttcaa ggactcgtcg tcctgcccga agtcggctgg
      541 acgggcacat tttacgtttg gggcgcaatc gccctgctga ttctgcctgt ctcatggtgg
      601 cttgccggcg gcaacaacgc cgcccacacc caacacgccc aagccacaca cggacaaagc
      661 ctcggagaag cagtcaaaac cgccttcaaa accccaagct acatcctgct gcacctaagc
      721 ttttttgcct gcggcttcca catcgccttt ctcgtaaccc acctacccac ggaagtcgcc
      781 ctgtgcggac tgcccgccac cgtcgcatcc acagccatcg ccatcatcgg actggcaaac
      841 atcgccggtt gtgtgttctc aggctggtgc acaagccgcg ttcggggcaa atacgtcctg
      901 ttcggactgt acgcctcgcg cgccgcgatg atactcatct acattttttc gcccaagacc
      961 gacctcaact tctacatttt cgccgccgca ctcggattta cctggctcgc caccgtcacg
     1021 ccgaccgcct ccattaccgg caaactcttc ggcacacgct acctcgccac cctgttcgga
     1081 ctgaagatgc tcagccacca aatcggcgga ttcctcggct cgtacatcgg cggcatcgtg
     1141 attacacaat tcggcgacta cggctggatg tggtacgccg acgcactgct ggcgggaacc
     1201 gccgccctcc tcaacctccc catccgcgaa ccccgaaccg ccgcatgaaa gaatgccgtc
     1261 tgaaaaccct cagacggcat tttccaaaca ccccgcccgt ttccggactg cacgacacgc
     1321 aaatcagcct tatcaaaccg caaacaaccg ccgcgattaa accgccattc aaataagtcc
     1381 gttttccctt cgggtgtaaa atcatcacaa aacgcgatac tgtatatcgc cgggcgttat
     1441 gcagcggatt tcaggataaa atgccgtctg aagccgcccc tgcccgggcg ggcgaagcgt
     1501 cggatgcggt gcgcttggca aaggtttgcc cgccgcgccc cgtatccggc atttcatcaa
     1561 tgcagaccgc atttccaatc ctataattca aggtgtccgc cttatgaaaa tcgtttttat
     1621 cacaacagtc gcatccagca tttacggttt ccgcgccccc gtcattaaaa aattaatcgg
     1681 caaaaaccat caggtgtatg cctttgtatc ggagttttcc gataatgaat tggatattat
     1741 cagggaaatg ggggttacac ccgttaccta ccgttcaaac cgcagcgggc tgaacccgtt
     1801 ttccgatata aaatccacct tcctcatatt taaagaactc aaaaaaatat cgccggattt
     1861 ggttttccct tatttcgcaa aacccgtgat tttcggcact tttgccgcaa aactggcagg
     1921 cgtgcccaga atcgtcggga tgctggaagg tttgggattc gcatttaccc cgcaaccgga
     1981 aggcataccg ttaaaaacaa aaatcataaa ggggattttg attgccttgt accgcattgc
     2041 cctgccgatg ttggaaagcc tgattgtatt aaaccccgac gacaaagacg aactgacgga
     2101 caaatacggc atcaaaataa aaaacatcca tattttgggc ggaatcggtc tggatttgcg
     2161 gcaatatcct tattccgagg cggatattcc cgatgaaaaa gaacccgtaa aattcctctt
     2221 tatcggcaga tttctgaaag aaaaggggat tgatgatttt attcgggcgg cggaacaggt
     2281 taaggacaaa taccccgata cggtttttac cgctttgggc gcaatcgaca aatca
//
LOCUS       DAWFAD010000205         2283 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWFAD010000205 DAWFAD010000000
VERSION     DAWFAD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.205"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            295..807
                     /locus_tag="WGO49_001947"
     CDS             295..807
                     /locus_tag="WGO49_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ0306999.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPEILLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            887..1966
                     /gene="aroB"
                     /locus_tag="WGO49_001948"
     CDS             887..1966
                     /gene="aroB"
                     /locus_tag="WGO49_001948"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ0307000.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPARELSAGMAEVIKYGALGDIGFFEWLEQHMPELMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          560 a          709 c          551 g          463 t
ORIGIN      
        1 ttgataaaca tattatcttc aatatattca attggataat tgtttaccta agcaaagata
       61 attgcctttt cctgacaaat aagtgaaatc aacggattgt caaaacacag cctgaaataa
      121 aaaacctccc tgatttcttt tatttgtcct taaaatcaga aaggttcggg atggtcgggt
      181 tatttttcca aacgtaccgc cgccctgccg atttcgtata aaattccgcc gtaacccgac
      241 aagcccgaac cctgtcgccc cgaaaggcgg ggtgtcaaac attaaggaat tgtgatgaaa
      301 aactttaacg gcaaactcat cctcatcgga ctgatgggcg cgggcaaaac cacgctgggc
      361 cggcaaatgg cgcagcggct ggattaccgt ttttacgaca gcgatcacga aatcgccgca
      421 gccgccggcg ttcccatccc caccatattt gaaatggaag gcgaacaggg attccgttcg
      481 cgcgaaaccg ccatactcaa aaagctggtt atcctgcccc atatcgtcct gtccaccggc
      541 ggcggcgcgg tgttaaaaga agaaaaccgc gcccttatcc gcaaaagcgg cacggtcgtc
      601 tatctgcacg ccccgcccga aatcctgctc gaacgcacgc gctgcgacaa cagccgtcct
      661 ttgctgcaag ttgccgatcc tttggcgaaa ttacgtgaac tctacgccgc acgcgacccc
      721 gtttaccgcc aaaccgccga ctttaccgta gaatcggcaa actgccggga aaccgtgcaa
      781 accctgctca aacgcttatc ccgataaacc ggcatatgcg ccgcgcccag aaaaccaaac
      841 cgcgccccgc ccggcgggcc ggcggttcaa actttaagga acaacaatga aaacactgac
      901 cgtacacacc ccgtcccaca gctaccccat ctttatcggc aacggactgc tgccgcaggc
      961 aggaagcctg ctcaaaccgc atttgggcaa acgcgccgcc atcatcgcca acgaaaccgt
     1021 cgccccgctc tacctcggca cgcttcagac ggcattggat gcggcaggcg tatcccattt
     1081 cagcatcatc ctgcccgacg gcgaggcgca caaaaactgg cagacgctca acctcatctt
     1141 tgacgggctg atgcaaaacc gcgccgaacg caaaaccaca ttaatcgcac tgggcggcgg
     1201 cgtgatcggc gacatggtcg gctttgccgc tgccacctac cagcgcggcg caccgttcgt
     1261 tcaaataccg accacactgt tgagtcaggt cgactcatcg gtgggcggca aaaccgccat
     1321 caaccacccg ctcggcaaaa atatgattgg cgcgttttac cagccgcagg cggtgcttgc
     1381 cgacttggac acgctgcaca ccctgcccgc ccgcgaattg tccgccggta tggcggaagt
     1441 catcaaatac ggcgcgctcg gcgacatcgg cttttttgaa tggctggaac agcatatgcc
     1501 cgaactgatg acgctcgatc gggaaaaact cgcccaagcc gtgtaccgct gctgccaaat
     1561 gaaggcagac atcgtcgccc aagacgaaac cgaacagggc atacgcgcat ggctcaacct
     1621 cggacacacc ttcggacacg ccattgaaac cgagatgggt tacggcactt ggctgcatgg
     1681 agaagccatc gccgccggct gcgtgttggc ggcgcgtttg tccgaacaac tgggcaaaac
     1741 ctccgccgca gataccgcgc ggctcgccgc cctgctcgaa gccgccggac tgccgtccgc
     1801 gccacccgtg tttgcctttg aaaaatggct ggaacacatg agccacgata aaaaagtcag
     1861 cggcggcatc atgcgcttta tcggtctgaa ccggctgggc gaagccaaca tcaccgaaat
     1921 taccgacacg gacatcctcc gccgcaccct gcaaccgtat ctctgatttc ctctgccgat
     1981 gtgctgccgc gcgggtttga cgcacgatga tgattttcca tcatctttct ccgcaaaagc
     2041 gggaatccag tccgttcggt ttcggtcgtt tccgataagt tcccgttgct tttcatttct
     2101 agattcccac tttcgcagga atgacggtgg aaaggttttt gttgtttcgg agaagtttct
     2161 gcaaccctag aatctcgtta tttccacaaa aaacagaaac ccgaaacagc aacttaaaac
     2221 ctcgtcattc ccgcaaaagc gggaatccag tccgttcggt ttcggtcgtt tccgataaat
     2281 tcc
//
LOCUS       DAWFAD010000206         2238 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWFAD010000206 DAWFAD010000000
VERSION     DAWFAD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.206"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..953
                     /locus_tag="WGO49_001949"
     CDS             <1..953
                     /locus_tag="WGO49_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0307001.1"
                     /translation="SGGRFYERCWLMGSEDVLYGRVHFGGQNNTILFELTGTGCGVAK
                     EGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRNKGMFTCHHVKPKGEC
                     LGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGDKTSTWTRFEIEFKAKDI
                     VIPFEVLQNPGEYFGGAYPICERFAQKATRIHAVKEDKVISADRYLEWVKKQFGRAAN
                     GLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKAQAIHEQPAFKPYKDPYY
                     MYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
     gene            998..1288
                     /locus_tag="WGO49_001950"
     CDS             998..1288
                     /locus_tag="WGO49_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307002.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1293..1490
                     /locus_tag="WGO49_001951"
     CDS             1293..1490
                     /locus_tag="WGO49_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307003.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            1498..1782
                     /locus_tag="WGO49_001952"
     CDS             1498..1782
                     /locus_tag="WGO49_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307004.1"
                     /translation="MFGGRLKNAPSITAKHFLKENIMKFINTCRKYGAKLAVVTAAPL
                     ALAAHANATLPDTAKNALEAAKADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            1789..2067
                     /locus_tag="WGO49_001953"
     CDS             1789..2067
                     /locus_tag="WGO49_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307005.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSLKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
BASE COUNT          673 a          519 c          511 g          535 t
ORIGIN      
        1 gctcgggcgg tcgtttctat gagcgctgtt ggttgatggg ttcggaagac gtcctatacg
       61 gtcgcgtcca ttttggcgga caaaacaata ccatcctttt cgaactgacc ggcaccggtt
      121 gcggcgtcgc aaaagaaggc tgggaatcac gacttttcgc attcctgact aatgcaatcc
      181 gcccaaaaat cacacgcgtt gacatcgcaa aagacttttt caacggcgaa tacagcccga
      241 accaagcccg tgaagaccga aataaaggta tgtttacctg tcatcacgtc aaaccaaaag
      301 gcgaatgttt ggggtcagat tgggaagaag acgatgaagc caaaatgacc aaaggcaaga
      361 cctatggtat cggctcccgt gaatcgtcca aatatgtccg cgtctatgaa aaaggcaagc
      421 agttgggcga taaaacaagc acatggacgc gctttgaaat tgaattcaaa gcaaaagaca
      481 tcgttatccc tttcgaagtt ttgcagaatc cgggcgaata tttcggcggc gcatatccga
      541 tttgcgaacg attcgcccaa aaggcaacgc gcatacacgc ggttaaggaa gataaggtca
      601 tttcagccga ccgctacctt gaatgggtaa aaaaacagtt cggacgtgcg gcaaacggtc
      661 tgaaattcat ttttcccgaa ttggacaaag ccaaactgtt tgaactgatt gagccgagtc
      721 atcacaagct gcccaagtct ttggctcccg aagcctacga ctgcgccttt ttgaaagctc
      781 aagccattca tgaacagccc gcattcaaac cgtacaaaga cccttactat atgtacgaat
      841 attacgagaa tcttgaaaaa cagcttgaac agcaaaaaca cgtcaacaat gaagaaagct
      901 ataacaactt catttacgac aaattcgcaa gactaccgat ttcatgggct taaagtgtct
      961 gcccgaaaga cgtttaatca cacaaggaaa ccaaaaaatg aacatccaac ttcaaggcca
     1021 catcgtcggc gttaaaaaat tcaacggaca aatcgaaggc aagagcttcg actattgccg
     1081 cctgattgtc gccacaccct tagacagctc ccaaggcaac gcattgggca gctctactac
     1141 tgaatacgat ttcggcggct ctgccaattt cgagcagttc cgaaacgccc aatttcccat
     1201 cgaagcaaac ctgaacgtgg aaatcgttac tacgggcaaa acccaaaaac tgaaagtcat
     1261 cggttttcaa ctcgtgaaga aaggctgatt gaatgcagaa agtctatgtt gtccagtccg
     1321 tatcaacagg ggactttctg tatctctctc ctgaaacggg cgacatcgga cataccaaat
     1381 taatcaccaa tgccgattat ttctacgact tcgaagaagc gattaacgca ggtttggaag
     1441 aaatcggcaa ccaatacgaa tttgtcgtat tcggattttt gaaagactga ttttcggatg
     1501 ttcggcggtc gtctgaaaaa cgctccatcc attaccgcca aacacttttt gaaggaaaat
     1561 atcatgaaat ttattaacac ctgccgtaaa tacggcgcaa aactggctgt tgtaacagct
     1621 gctcccctgg ctttggccgc acatgcaaat gcaacgttgc ccgatacggc aaaaaacgct
     1681 ttggaagccg caaaagcgga cggtatggaa gccggttgga ttgtcgttgg cgttttcgcc
     1741 gcgctttttg tattttccat cgttaagaga gtgatgaagt aagacggcat gtactaccaa
     1801 gtcggaaata aatgtcttga gaagcaccag gctgaaaacc tttatttcag cttggtagta
     1861 ccaagaatca aagaaaacgg acagattgtc aggccggaat ataacggcag cctgtggaag
     1921 atgtcggacg gtcagccgct aaggctttta ttggcggaat gcagtctgaa agacaacctg
     1981 caaagcggcc ttgaaacagg ctggatagta ttcggcatcc tcgcgtccgt ttactttgtt
     2041 tccctgctga aaaaggtttt gaaatgatgg atttttattt ttatctcggc gtttccgtac
     2101 ccgtattaat cggggcggtt ctgtttaaga attgagcgca tgaagttatg gtgtcaaaat
     2161 caggctttca aaacaatcat tgaaaggcag aaccatgaac aagccgttta tcactcaggc
     2221 gcagttggca ctttataa
//
LOCUS       DAWFAD010000207         2170 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWFAD010000207 DAWFAD010000000
VERSION     DAWFAD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.207"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            99..1910
                     /gene="typA"
                     /locus_tag="WGO49_001954"
     CDS             99..1910
                     /gene="typA"
                     /locus_tag="WGO49_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ0307006.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESSDMRPLFDTILKHTPAPSGSADEPLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
BASE COUNT          543 a          630 c          570 g          427 t
ORIGIN      
        1 gcaagggctt cagacggcat cttcatttcc caaatactgt ccggtaaagc gtggtataat
       61 gcgcagttat ctcttacagt ttttgaaaaa cattaattat gaaacaaatc cgcaacatcg
      121 ccatcatcgc ccacgtcgac cacggcaaaa ccacattggt cgaccaactg ctgcgccaat
      181 ccggcacatt ccgcgccaac cagcaggttg acgagcgcgt gatggacagc aacgacctcg
      241 aaaaagaacg cggcatcacc atcctcgcca aaaacaccgc catcgattac gaaggctacc
      301 acatcaatat cgtcgatacg ccgggacacg ccgacttcgg cggcgaagta gagcgcgttt
      361 tggggatggt ggactgcgtc gtcttgttgg tggacgcgca ggaaggcccg atgccgcaaa
      421 cccgtttcgt gaccaaaaaa gccttggctt tggggctgaa accgattgtc gtcatcaata
      481 aaatcgacaa gccgtctgca cgtccgagct gggttatcga ccaaactttc gagctgttcg
      541 acaacttggg cgcgaccgac gagcagttgg atttcccgat tgtttacgct tcaggtttga
      601 gcggctttgc caagctggaa gaaaccgacg agagcagcga tatgcgcccg ctgttcgaca
      661 ccatcttaaa acacacgccc gcaccgagcg gcagcgcgga cgagccgctg caactgcaaa
      721 tttcccaact cgactacgac aactacaccg gccgcctcgg catcggtcgt atcttgaacg
      781 gacgcatcaa acccggccaa accgttgccg tcatgaacca cgagcagcaa atcgcccaag
      841 gccgcatcaa ccagcttttg ggtttcaaag gcttggaacg cgtaccgctt gaagaagccg
      901 aagccggcga catcgtgatt atttccggta tcgaagacat cggcatcggc gtaaccatca
      961 ccgacaaaga caaccccaaa ggcctgccga tgttgagcgt ggacgaaccg acgctgacga
     1021 tggactttat ggtcaacacc agcccgctgg cgggtacgga aggcaaattc gtaaccagcc
     1081 gccaaatccg cgaccgcctg caaaaagaat tgctgaccaa cgtcgccctg cgcgtggaag
     1141 acaccgccga tgccgacgtg ttccgcgtat ccggacgcgg cgagctgcac ctgaccattt
     1201 tgttggaaaa tatgcgccgc gaaggctacg aactcgccgt cggcaaaccg cgcgtcgtgt
     1261 accgcgacat cgacggtcaa aaatgcgaac cttatgaaaa cctgaccgtg gacgtacccg
     1321 acgacaacca aggcgcggta atggaagaac tcggccgccg ccgtggcgaa ctgactaata
     1381 tggaaagcga cggcaacgga cgcacccgcc tcgaatacca tattccagcg cgcggcttga
     1441 tcggtttcca aggcgaattt atgaccctga cgcgcggggt cgggctgatg agccacgtgt
     1501 tcgacgatta cgcgcccgtc aaacccgata tgcccggccg ccacaacggc gtgctggtgt
     1561 cccaagagca gggcgaggca gtcgcttacg ccttgtggaa tctggaagac cgcggccgta
     1621 tgttcgtatc gcccaacgac aaaatctacg aaggtatgat tatcggcatc cacagtcgcg
     1681 acaacgattt ggtggtcaac ccgctcaaag gcaaaaaact taccaacatc cgtgccagcg
     1741 gtaccgacga agccgttcgc ctgaccacgc cgattaagct gacgctggaa ggtgcggtcg
     1801 agtttatcga cgatgatgag ctggtagaaa tcacgccgca atccatccgc ctgcgcaagc
     1861 gttacctgag cgaattggaa cgccgccgcc atttcaaaaa gctagattga tgtttactgg
     1921 atttaatgtt taaatgatac tcgatgccgt ctgaaaaagt tcagacggca ttttttattc
     1981 ggacgggctt tgcggcttct tgaagctgtt tcagacggcg tttttcctac ccaatcaaga
     2041 aactgccgcc atttttccag cggcatatcg ccccgtcgcg tttcggtatc gggttcggct
     2101 tcccggcagc atgtcgaaca atgccgtttg aaggaatacg ccttttgagt ccttacaagc
     2161 ttaggaagag
//
LOCUS       DAWFAD010000208         2124 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWFAD010000208 DAWFAD010000000
VERSION     DAWFAD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.208"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            122..1507
                     /locus_tag="WGO49_001955"
     CDS             122..1507
                     /locus_tag="WGO49_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ0307007.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFSIV"
     gene            1527..2045
                     /locus_tag="WGO49_001956"
     CDS             1527..2045
                     /locus_tag="WGO49_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ0307008.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
BASE COUNT          446 a          645 c          571 g          462 t
ORIGIN      
        1 ttcaaataaa caggaatata acttcagaca aacaacttac cgcccgattt gtgctatcgt
       61 tttcgcacaa cttaaaaaac ctgacaattt tgtactttta ttacagagaa aggctttaca
      121 aatggacggc tggacacaga cgctgtccgc gcaaaccctg ttgggcattt cggcggcggc
      181 aatcatcctc attctgattt taatcgtcaa attccgcatc cacgcgctgc tgacactggt
      241 catcgtcagc ctgctgacgg ctttggcaac cggtttgccc acaggcagca ttgtcaacga
      301 cgtactggtc aaaaacttcg gcggcacgct cggcggcgtg gcgcttctgg tcggcctggg
      361 cgcaatgctc ggacgtttgg tcgaaacatc cggcggcgca cagtcgctgg cggacgcgct
      421 gatccggatg ttcggcgaaa aacgcgcacc gttcgcgctg ggcgttgcct cgctgatttt
      481 cggcttcccg attttcttcg atgccggact aatcgtcatg ctgcccatcg tgttcgccac
      541 cgcacggcgc atgaaacagg acgtactgcc cttcgcgctt gcctccatcg gcgcattttc
      601 cgtcatgcac gtcttcctgc cgccccatcc gggcccgatt gccgcttccg aattttacgg
      661 cgcgaacatc ggccaagttt tgattttggg tctgccgacc gccttcatca catggtattt
      721 cagcggctat atgctcggca aagtgttggg gcgcaccatc catgttcccg ttcccgaact
      781 gctcagcggc ggcacgcaag acaacgacct gccgaaagaa cctgccaaag caggaacggt
      841 cgtcgccatc atgctgattc ccatgctgct gattttcctg aataccggcg tatcggccct
      901 catcagcgaa aaactcgtaa gtgcggacga aacctgggtt cagacggcaa aaataatcgg
      961 ttcgacaccg atcgcccttc tgatttccgt attggtcgca ctgtttgtct tgggacgcaa
     1021 acgcggcgaa agcggcagcg cgttggaaaa aaccgtggac ggcgcactcg cccccgtctg
     1081 ttccgtgatt ctgattaccg gcgcgggcgg tatgttcggc ggcgttttgc gcgcttccgg
     1141 catcggcaag gcactcgccg acagcatggc ggatttgggc attcccgtcc ttttgggctg
     1201 tttccttgtc gccttggcac tgcgtatcgc gcaaggttcg gcaaccgtcg ccctgaccac
     1261 cgctgccgcg ctgatggctc ctgccgttgc cgccgccggc tttaccgact ggcagctcgc
     1321 ctgtatcgta ttggcaacgg cggcaggttc ggtcggttgc agccacttca acgactccgg
     1381 cttctggctg gtcggccgcc tcttggacat ggacgtaccg accacgctga aaacctggac
     1441 ggtcaaccaa accctcatcg cactcatcgg ctttgccttg tccgcactgc tgttctccat
     1501 cgtctgacag acggaaagga tagtaaatga ctacgcattt tgtcgttatg ggcgtatgcg
     1561 gctgcggcaa gaccaccgcc gcgctgtccc tgcagaaaca cctcggtcaa tgtccctatg
     1621 ccgaaggcga cgagttccac acccaagcca accgcgacaa gatgggcgcg ggtattccgc
     1681 tgaccgatga agaccgctat ccgtggttgg gcaatctgcg cgactggatg acgcaacagg
     1741 cgcaaaacgg tgcgaaccac accatcgtaa cctgttccgc cctcaaacgc ggctaccgcg
     1801 acattctgcg cggagccgaa ggcaaagctg ccttcatcca cctcagtccg ccgcaagaca
     1861 tcaacctcga gcgcatgatg tcgcgcaaag gacattatat gaaagcaggg atgctcgatt
     1921 cgcaactgga aatcctcgag gaactgggcg aaggcgaata cggggtcaaa atcgccaacc
     1981 ccggcacgcc cgaagcggtc gaagccgata ttctgaactg ggttgcctcg gaaaacctgc
     2041 tttgaagcaa tatgccgtct gaagcccgac acaggatggg tttcagacgg cataaacatc
     2101 gggaacagaa tggattacat tgat
//
LOCUS       DAWFAD010000209         1982 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWFAD010000209 DAWFAD010000000
VERSION     DAWFAD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.209"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            372..1457
                     /locus_tag="WGO49_001957"
     CDS             372..1457
                     /locus_tag="WGO49_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0307009.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVEVSRVKDAGTYKA
                     QGGKSKTATQIADFGSKIGFKGQEDFGNGMKAIWQLEQKASIAGTDSGWGNRQSFIGL
                     KGGFGTVRAGNLNTVLKDSGDNVNAWESGSNTEDVLQLGTIGRVESREISVRYDSPVF
                     AGFSGSVQYVPRDNANDKDKYDHKLSSRESYHAGLKYENAGFFGQYAGSFAKYADLNN
                     DAKRVAVGAVGSNPVKDYQVHRVVAGYDANDLYVSVAGQYEAAKNNEVGSNNIGKKHE
                     QTQVAATAAYRFGNVTPRVSYAHGFKAKVNGVKEASYQYDQVIVGADYDFSKRTSALV
                     SAGWLKQGKGEDKVQQTASMVGLRHKF"
BASE COUNT          566 a          497 c          447 g          472 t
ORIGIN      
        1 tcctgccgca gggtcgggtt ttgcatccgc ccgttccgaa agaaaccacg tgcgcgtttt
       61 ttgccgtctt tataaccccc ggtttgcaat gccctccaat accctcccga gtaagtgttg
      121 taaaaatgca aatcttaaaa aatttaaata accatatgtt ataaaacaaa aaatacccac
      181 aatatctcta tccgcccttc aaaatacaca tcgaattcca cacaaaaaca ggcagaagtt
      241 tgttttttca gacaggaaca tctatagttt cagacatgga atcgccgaaa acgtcggcgg
      301 taaatgcaaa gctaagcggc ttggaaagcc cggctggctt aaatttctta actatgaaaa
      361 ggaatacagc aatgaaaaaa tccctgattg ccctgacact ggctgccctg cctgttgcag
      421 caatggctga cgttaccttg tacggtacaa ttaaagcagg cgtagaagtt tctcgcgtaa
      481 aagatgctgg tacatataaa gctcaaggcg gaaaatctaa aactgcaacc caaattgccg
      541 acttcggttc taaaatcggt ttcaaaggtc aagaagactt cggcaacggc atgaaagcca
      601 tttggcagtt ggaacaaaaa gcctccatcg ccggcactga cagcggctgg ggtaaccgcc
      661 aatccttcat cggcttgaaa ggcggcttcg gtaccgtccg cgccggtaat ctgaacaccg
      721 tattgaaaga cagcggcgac aacgtcaatg catgggaatc tggttctaac accgaagatg
      781 tactgcaact gggtactatc ggtcgtgtag aaagccgtga aatctccgta cgctacgact
      841 ctcccgtatt tgcaggcttc agcggcagcg tacaatacgt tccgcgcgac aatgcgaatg
      901 ataaggataa atacgaccat aagctgtcca gccgtgagtc ttaccacgcc ggtctgaaat
      961 acgaaaatgc cggtttcttc ggtcaatacg ccggctcttt tgccaaatat gctgatttga
     1021 acaatgatgc aaaacgtgtt gcagtaggtg ctgtaggtag caatcctgtt aaggattacc
     1081 aagtacaccg cgtagttgcc ggttacgatg ccaatgacct gtacgtttct gttgctggtc
     1141 agtatgaagc tgccaaaaac aacgaggttg gttctaacaa tattggtaaa aaacacgagc
     1201 aaactcaagt tgccgctact gccgcttacc gttttggcaa cgtaacgcct cgcgtttctt
     1261 acgctcacgg cttcaaagct aaagtgaatg gcgtgaaaga agcaagttac caatacgacc
     1321 aagttatcgt tggtgccgac tacgacttct ccaaacgcac ttccgctctg gtttctgccg
     1381 gttggttgaa acaaggcaaa ggcgaggaca aagtccaaca aactgccagc atggttggtc
     1441 tgcgtcacaa attctaatct gaaaagattg gtgtaaaaaa aagcctgtcg aaagacaggc
     1501 ttttttattt gaaatgattc gtttgctatt taatacggca gccttcattc ctacgagagt
     1561 gggcatttat cgggaaagca gaaacttctc cgccgtcatg cccgcgcagg cgggaatccg
     1621 aacacgtccg catggaaacc tgcatcccgt cattcccacg aaagtgggaa tccagaacgc
     1681 agggttgaag aaactatttt atttgataag tttccgcact gacagaccta gattcccgcc
     1741 tgcgcgggaa tgacaaattc atccgtacgg aaacctgcac cgcgtcattc ccatgaacct
     1801 acattccgtc attcccacga acctgcatcc cgtcattccc acgaaagtgg gaatccagga
     1861 cgcaaaatct caagaaaccg ttttacctga taagtttccg tgccgacaga cctagattcc
     1921 cgcctgcgcg ggaatgacgg cggagcggtt tctgtttttt ccggtaaata cccacaagct
     1981 aa
//
LOCUS       DAWFAD010000210         1947 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWFAD010000210 DAWFAD010000000
VERSION     DAWFAD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.210"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            247..1704
                     /locus_tag="WGO49_001958"
     CDS             247..1704
                     /locus_tag="WGO49_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0307010.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPIVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFIPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKCYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          439 a          579 c          443 g          486 t
ORIGIN      
        1 aaaccgccct atcggataag cccttaacag aaaaggcttt acccgcgccg tatcggaaca
       61 catcctctaa aatacaatcc gttgaattga aaaaaatata aaaacatccg cccgcgaaaa
      121 acggcagcgc gtcgtttgac aaagaatgaa aatatcggtt aaaaaccgat tttcatacaa
      181 aaaacaccgc tgccgtccgc atccgtttca gacggtattg agagaaaatc ttttaggaga
      241 acctttatgt cccggcatcc cgcccccacc ggagaaaaaa cattcttcgg ccaccccttc
      301 cagctttcca ccctcttcca tatcgaattg tgggaacgtt tttcatttta cggaatgcag
      361 ggcatcctgc tgatttacct ctactacacc gccgacaaag gcggcttggg catagacaaa
      421 accctcgccg gcggcattgt cggcgcatac agcggcagcg tgtacctgtc caccattttg
      481 ggggcgtggt ttgccgaccg agtatggggt gcggaaaaaa ccctcttcct ctcgggcatc
      541 gtcgtgatgc tcggacacat cgtccttgcc gccgccccgg gcctgtacgg ccttttaatc
      601 gggctgatat tcatcgcatt gggcagcggc ggcgtgaaat ctacggccag ttctatggtg
      661 ggcgcattat acgaacagga cgaaatgcgc ccgctgcgcg atgcgggatt ttccattttc
      721 tacatcgcca tcaatatcgg cggcttccta ggcccgctgc tgaccggcct gctgcaggaa
      781 aacatcggtt tccattatgg tttcggcgcg gcggcggtcg gtatggcatt cggcttgtgg
      841 cgttattcct tgggacgtaa aaacctgccc catcccatcg tcccccatcc gctttcaaaa
      901 ggacagggca aaactgcggc cgccgtcggc attgctctca tcgccgcact tgcaaccgcc
      961 atcaaaaccg ggcttgtcaa cctcgacaat ttctccggca tcctcttatc taccgtcatc
     1021 cttgccgtca ttgcctattt tgcccgcctg ctgactaatc cccgcgtcag ttccgacaac
     1081 aaacggcaca tcatcgccta catcccgctt ttcctgacca tctgtatgtt ttgggccgtc
     1141 tggtttcaga tttacaccgt agcaaccgtc tatttcgacg aaaccgtcaa ccgcaccatc
     1201 ggttcgttta ccgtgcccgt cgcctggaaa gattctatgc aaagcctgtg ggtcatcctg
     1261 ttttccggac ttatggcggc aatgtggaca aaaatggggc gcaaacagcc caaaaccccg
     1321 ctgaaattcg ctatggcggt atttgttacc ggcgcgtcgt ttttgggatt catccccttt
     1381 atttccgccg gcacgccgat gcctattgcg gttttcgcac tgattgtcct tgccatcacg
     1441 ataggcgaac tgatgatttc cccgattgcg ctgtccatct ccaccaaaat cgcaccgcct
     1501 ttattcaaaa cccaaatggt cgcccttaat ttccttgcct tttcattagg cttcactttg
     1561 ggcggcgtat tgtttgaaaa atgctatcag gcgggcgacg aaatcggctt ctatcggctg
     1621 ctgttctaca tcggcgcagc cacaggcttc ctgctgctcc tgctcgtccc caaattgaac
     1681 aaaatgctcg aaggcacaga ctaagtcccg ccccgatgcc gtctgaaccc ttcagacggc
     1741 atttttccgc ataatgaaac caaaccgttt ccacccgaca ggacaggctc cccccaaccg
     1801 gaaggcagcc tgccgattgt catttaaata tctgactgat tccgtctgtt tgcaagggaa
     1861 atagccgttt atttccgctt gggcggaaac cggttctatt gagtaaaagg cattttatcc
     1921 gactaaatta gtgctgcatc aacggaa
//
LOCUS       DAWFAD010000211         1945 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWFAD010000211 DAWFAD010000000
VERSION     DAWFAD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.211"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(42..365)
                     /locus_tag="WGO49_001959"
     CDS             complement(42..365)
                     /locus_tag="WGO49_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0307011.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKARLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(353..592)
                     /locus_tag="WGO49_001960"
     CDS             complement(353..592)
                     /locus_tag="WGO49_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ0307012.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(715..1512)
                     /gene="truA"
                     /locus_tag="WGO49_001961"
     CDS             complement(715..1512)
                     /gene="truA"
                     /locus_tag="WGO49_001961"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ0307013.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQAGGVPTVQTALETALAQI
                     AGESVATTVAGRTDTGVHATAQVVHFDTTAVRPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESVPVRSPLLKNRAGWTHLKLDIGPMRRAAALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          437 a          458 c          553 g          497 t
ORIGIN      
        1 ttaatccact ataatgaaca caatcaattc agactattca atcaggcaaa catctcctgc
       61 aatactgcaa acagtttttc agccgtactg ttgtctaaat ttccaagatg cttgaccaat
      121 ctggctttat ccacagccct aatctgttcg ggcaaaagca aaccgtcttt atcctgaaag
      181 cggacattga cgcggaacgg ggcaggacgg cttccgctcg tcataggaac gatcagcaca
      241 gtcttgagat agttgtgtat ttcaggagga gatactacga cacaaggacg tgtctttttg
      301 atttcgcttc ctacggtcgg gtctaaggag accagataga ttccgccgcg tactaccata
      361 tccattcttt atccgcttcg ttttcaattt cggaaaaaaa atgctcctgc tcggtttcga
      421 caagcattgc ggcagcttct gcccatcccc tgcgaacggt aggacagctt aaaatgatat
      481 tgcccttttc aactgtaaca gccaagctgt ctactgcccc tatttgaccc aataatgatt
      541 tgggcagaat cacgccttgc gagtttccca ttttgcgtat gttgagaatc atatacgtac
      601 catattcacc agtttatgta ataacaatgt tagtaccttg atgaggtagt gtcaacattg
      661 aaaaagattg cctgacagtt tgtccgattt caaaatctcc gcgacaagaa tgttttaaag
      721 ccattcgggg atttgggggc ggatgatgcc gtatgcctcg ggatagtcga cgccggtcag
      781 gtaaagtccg tcgggcatga aggtcggcgg ggctttgagg cggctgcgtt cttgaatcag
      841 tgcggcgaag ccttcgacgc tgagtctgcc gctgccgaca tagacgagcg cgcccatgat
      901 gttgcgtacc atatggtgca aaaaggcgtt gccgtgcaaa tcgaggcgga cgagtcctga
      961 gctttgggta aggtcggcgc ggtagatggt tttgacgggg gattttgcct ggcattcggc
     1021 ggcgcggaag ctggagaagt cttgttcgcc gatcaataag gcggcagccc ggcgcatcgg
     1081 cccgatgtcg agtttgaggt gtgtccagcc tgccctgttt ttgagcaggg gggaacggac
     1141 ggggacggat tcgagcaggt agcggtaatg ccgtccgtat gcgtcgaagc gggcgtgaaa
     1201 ttcgggggcg acctgtcggg cgtgcaaaac ggcaatgcct tcgggcaggt gggcatttac
     1261 gccgcgcacc catgcctgtt ggggacggac ggcagttgtg tcgaagtgga cgacttgggc
     1321 ggtggcgtgc acgccggtgt cggttctgcc ggcaacagtg gtggcgacag attctcctgc
     1381 gatttgggcg agcgcggttt ccaatgccgt ctgaacggtc ggtacgccgc cagcctgttt
     1441 ctgccagccg taaaagcggc tgccatcata ggatagggtt attgcccagc gttgtttttg
     1501 tgcggtatcc atcggatttg ggattcggat aaatgttcag actgcattgt atcgcagatt
     1561 ttgcagggaa acggcaaatg cccggggcgc gcggcgttgt ttggggagtt gggggggggg
     1621 atgcagttgc tacgaatcgc tatcctgtga attcaccctg tcaggagtgc ccgaatcgtc
     1681 attcccgcgc agtcgggaat ctaggacgta aaatctaaag aaaccatttt atagtggatt
     1741 aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaatgattc tctaaggtgc
     1801 tgaagcacga gtgaatcggt tccgtattat ttgtactgtc tgcggcttcg tcgccttgtc
     1861 ctgatttttg ttaatccact atatccgata agtttccgca ccgacaaaac tagattccca
     1921 ctttcgtggg aatgacggga tgcag
//
LOCUS       DAWFAD010000212         1902 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWFAD010000212 DAWFAD010000000
VERSION     DAWFAD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.212"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..1037)
                     /locus_tag="WGO49_001962"
     CDS             complement(<1..1037)
                     /locus_tag="WGO49_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307014.1"
                     /translation="MTSANFNINGFGDVKLTPYSPLLGYKAWDSFIGSIQSLSDLIYN
                     VDNNRNKMEITVNNAIQAADSFLSSIGRDNKITNTASLLASLDNIFLNLRNVSRDIRE
                     TGKFKPNDIQQAIGDIFIAAGDGLQYIKQQTEAMAQSKFLPTKLKTGLNDVLNSRMLK
                     SSTVLQHELNYLGFKIKDYGNERLGESIMNIDDFTPSKIANFFADPDTYSNVLEEVSR
                     FIYSLVPDDANPWKGGEDYIGRGISEWGELLEKWYKQDFLPYLEKEWDQFPKFEDWLP
                     EFPEWAREWLKLDPKRSGKYHVYDPLALDLDGDGIETVAAKGFAGALFDHRNQGIRTA
                     TGWVSADDGLLV"
BASE COUNT          635 a          340 c          285 g          642 t
ORIGIN      
        1 acgagtaaac cgtcatcggc agaaacccaa ccggtggcgg tgcggatgcc ttgattgcgg
       61 tggtcgaaca atgcacctgc aaagcctttg gcagcaacgg tttctatacc gtcgccgtct
      121 agatctaggg cgagggggtc gtagacatga tatttgcctg aacgtttggg atctaatttc
      181 aaccactctc ttgcccattc agggaattca ggcagccaat cttcaaattt cggaaattgg
      241 tcccattctt tttcaagata agggagaaaa tcttgtttat accatttttc cagtaactct
      301 ccccattcac ttatccctcg tccaatataa tcttcgcccc ctttccaagg gtttgcatca
      361 tcaggaacta aggaatatat aaacctagat acttcttcta atacattgct gtatgtatca
      421 ggatccgcaa aaaagtttgc tatcttactt ggtgtaaaat catctatatt cattatagat
      481 tcgccaagcc tctcgtttcc ataatccttt attttaaatc ccaaataatt caattcatgc
      541 tgtaaaacag tagaggattt tagcattcta gaattaagga catcatttaa accagttttt
      601 aatttagttg gtaagaattt gctttgagcc atcgcctctg tttgttgttt tatatattgt
      661 aatccatcac cagcagcaat gaatatatca ccaattgctt gttgaatatc attaggttta
      721 aattttcctg tttctcgtat atctcgagat acatttctta aatttaaaaa aatgttatcg
      781 agggatgcaa gtaaagaagc agtatttgtt attttgttat ctcttccaat actgcttaaa
      841 aagctatctg cagcttggat agcattatta acagtaattt ccattttatt tctattgtta
      901 tccacattat agattaaatc agataaggat tgaatagaac caataaatga atcccaagct
      961 ttatatccca agagtggtga atagggtgtt aatttcacat ctccaaaacc gttaatatta
     1021 aaatttgcag atgtcatatc tgctttcctt tcaagtaaat taaaaaaata acgtgaggaa
     1081 ttaaaattaa gaataaatta attatcgact caatataata tgaatcaatt agctctctta
     1141 tggtaaatat aagaggtaaa aatattaaaa ataaactaat aaagttcaga aaaatagtaa
     1201 atggaatatt ttcatatgga aaaattctac aaaatacaag taatattcca tgacatatta
     1261 gcaaatgagt aaaatttatt ataaaatata taaatatttc cccatcgtaa gatatattca
     1321 aataaagaca tataaacaat atgaagtata taaaatatat tttttggcat ctaaaaaata
     1381 aacttttagc aatcatatat aaatagcttg tagaataaaa aacgaaaatt attaattatc
     1441 gtaggtgtgc atttattcca acacggtttc atgcagctta aaaaccaaaa attctttctc
     1501 cctttatcct ttcttaaaaa tagcgttttc aaattctgca acgacaggtt tcattcaagt
     1561 aggactgccg tgattgcatc aattgcttcg cggcatggca tccatatcac acaacaggac
     1621 atttattctc gggtaagtcc accttcatcc tcttccgcca gcaggaacag ttacaggcta
     1681 cctaaaatat gctctgtgct cggatagtaa atatttcctt tctcactatt gtttgtcatt
     1741 taatcgacag tctactcttt ctctaaaatt tcgtcaataa tagttaaata cttttattta
     1801 ttttaaataa tgatatacat ttatggttat ttgtttagac agcctaaaac aaacggggaa
     1861 atcgtgtttt cccgaacatg gctaatggat agcattttgt tc
//
LOCUS       DAWFAD010000213         1880 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWFAD010000213 DAWFAD010000000
VERSION     DAWFAD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.213"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            566..1381
                     /locus_tag="WGO49_001963"
     CDS             566..1381
                     /locus_tag="WGO49_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FrpC operon protein"
                     /protein_id="HEZ0307015.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNNRINQTEED
                     SYSTSVGSCINGFTVQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSNKK"
     gene            1398..>1880
                     /locus_tag="WGO49_001964"
     CDS             1398..>1880
                     /locus_tag="WGO49_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307016.1"
                     /translation="MNEGEVVLTPEQIQTLRGYASRGDTYGGWRYLANLGDRYADNAA
                     AIVGKDTNLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINKNNGR
                     LPNTSEIERSYYKAVTYHGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQA
                     V"
BASE COUNT          629 a          305 c          385 g          561 t
ORIGIN      
        1 aaatatacca aaaacagcaa aacaaattgt aaggatacgt ataggctttg taaaggtaga
       61 ttgtgaaaaa agcagttttt taaacgaatg aaacggcttc gggctgaaat atatgctgat
      121 gccctgtcct tcccgtatat cttgtgtgtt gtcaaagtgc aggctgcttt gaaattggca
      181 ttacagtcga tgaaccacca ttttgcttta tttcagcggg cttgagatgt gtataagaat
      241 attgttttga ataaatttaa agaaaatgat aatcgttatt gacgattttt aaaggaaagc
      301 gtagagtgcc aattctatga agcaatacgg taagtaacaa tgaaaatatc tactgcttgg
      361 gtatagagca tatttcacaa cccgtaacta ttcttgcgga aacagagaaa aaagtttctc
      421 ttctatcttg gataaatata tttaccctca gtttagttaa gtattggaat ttatacctaa
      481 gtagtaaaag ttagtaaatt atttttaact aaagagttag tatctaccat aatatattct
      541 ttaactaatt tctaggcttg aaattatgag accatatgct actaccattt atcaactttt
      601 tattttgttt attgggagtg tttttactat gacctcatgt gaacctgtga atgaaaagac
      661 agatcaaaaa gcagtaagtg cgcaacaggc taaagaacaa accagtttca acaatcccga
      721 gccaatgaca ggatttgaac atacggttac atttgatttt cagggcacca aaatggttat
      781 cccctatggc tatcttgcac ggtatacgca agacaatgcc acaaaatggc tttctgacac
      841 gcccgggcag gatgcttact ccattaattt gatagagatt agcgtctatt acaaaaaaac
      901 cgaccaaggc tgggttcttg agccatacaa ccagcaaaac aaagcacact ttatccaatt
      961 tctacgcgat ggtttggata gcgtggacga tattgttatc cgaaaagatg cgtgtagttt
     1021 aagtacgact atgggagaaa gattgcttac ttacggggtt aaaaaaatgc catctgccta
     1081 tcctgaatat gaagcttatg aagataaaag acatattcct gaaaatccat attttcatga
     1141 attttactat attaaaaaag gagaaaatcc ggcgattatt actcatcgga ataatcgaat
     1201 aaaccaaact gaagaagata gttatagcac tagcgtaggt tcctgtatta acggtttcac
     1261 ggtacagtat tacccgttta ttcgggaaaa gcagcagctc acacagcagg agttggtagg
     1321 ttatcaccaa caagtagagc aattggtaca gagttttgta aacaattcaa ataaaaaata
     1381 atttaaagga tcttattatg aatgagggtg aagttgtttt aacaccagaa caaatccaaa
     1441 ccttacgtgg ttatgcttcc cgtggcgata cctatggcgg ttggcgttat ttggctaatt
     1501 tgggcgaccg ttatgcggat aatgctgctg caattgtcgg taaggataca aacttaaatg
     1561 gtttgaattt atggatgaaa aaaggggtgg aaaacctatg ggatgatacg gtcggtaaaa
     1621 agacccgttt agagaaattt gatcgggttg cattgcaaca tttcagccaa tatgtagatc
     1681 taattaataa aaataatggt agattaccta acactagtga aattgagaga agttactata
     1741 aagccgttac ctatcatggc gtttcttcta gtgcagctat tgatttagtt attaatcgtt
     1801 cacttccgga tatggcggat ggttattggg cattaggttt ggggatagaa gccgaacgta
     1861 tccacaatga gcaagcagta
//
LOCUS       DAWFAD010000214         1831 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWFAD010000214 DAWFAD010000000
VERSION     DAWFAD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.214"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            54..1223
                     /gene="metZ"
                     /locus_tag="WGO49_001965"
     CDS             54..1223
                     /gene="metZ"
                     /locus_tag="WGO49_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ0307017.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VVSSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVANLEALAELAHGIGAILVVDNSLLSPIGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQAAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAALIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1309..1515
                     /locus_tag="WGO49_001966"
     CDS             1309..1515
                     /locus_tag="WGO49_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ0307018.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
BASE COUNT          440 a          488 c          474 g          429 t
ORIGIN      
        1 taaacaacgg gcgcacgctc cgttgccgaa cggataaaac cgaggaatta ccgatgagca
       61 aaaaactcca tccgcaaacg ctcgccatac gcggcggcaa agaacaaacc gaataccgcg
      121 aacacaatca ggccttgttc ctgaccagca gcttcatgtg ggacaacgcc caacacgccg
      181 ccgatttgtt ttcaaaaaaa atcaaaggct tcacttatac ccgtaccgcc aacccgacca
      241 cagccgcctt tgaaaaacgc atcgccgctt tggaaggtgc ggaacgcgcg gttgccactt
      301 cgaccggcat gtccgcgatt caggcggcgt ttttcacctt tttgcaggcg ggcgaccatg
      361 tggtttccag ccgcagcctg ttcggcacga ccgtcggctt tatcaataac atcgttacca
      421 aattcggcat cgaagtgagc cacgtgtcgc cgactgatat aaacgaatgg aaagccgccg
      481 tcaaagccaa taccaaactg ctgtttttgg aaacgccgtc caaccccttg ggcgaagtgg
      541 ccaacttgga ggcattggcg gaattggcgc acggcatcgg cgcgattttg gtggtggaca
      601 acagcctgct gtcgcccatc ggctctcagc ctttgaagca cggcgcagat atttccgttt
      661 cttccgcaac caaagccata gacggacacg gacgcgtgat gggcggcgtg ttggcgggtt
      721 cggaagaact gatggcgcag gccgccgttt actgcaactc ttgcgggctg gcgatgtcgc
      781 cgtttaacgc gtggcagttg ttgagcggcg tggaaaccct gtcgctgcgt atggaaaaac
      841 agttcgacaa cgccctgaaa atcgcacaat ggctgcaagc gcagccgcag gttcaagccg
      901 tgtactacac cggattgccc gaccatccgc aggcggcgct tatccgcaaa cagcaaaacg
      961 gcggcggcat cgtcatcggc tttgaagtcg ccgaccaagc agccgcgtgg aaagtcgtgg
     1021 acggcgtgga actcttttcc cgtaccgcca acctcggcga cgtgcgctcg accatcaccc
     1081 atccgtggac aaccacgcac ggcaggatgc agcccgaaga aaaactcgcc gccaacatcc
     1141 gccccggatt ggtgcgcctg tcggtcggtt tggaatatgt tggcgatttg attgacgatt
     1201 tgaaacaggc actcgcacgc tgattgcgtg tttcagacgg cattggacag cagatgccgt
     1261 ctgaaaccct tatcgcgtat ggcatacaca tcaacaatag gaaaaaatat ggatgaagcg
     1321 gtttttgccg attgggcgtt gaaaatctgc ctgaccggtt tgattatttt tttgggtttt
     1381 atcgtttgga atttgggcaa ggagtccaaa gcgggcaaat tcggcatcgc cattttgttt
     1441 ttggttttgg gcttgggcgt gttcggtttt gtatttaaag aactgctaat caagtttttg
     1501 gtattgccca aataggctgc cgcctgtcaa aatgctgtct gaaatcataa aaagtaatct
     1561 gcaccctaaa agtttgattt aaccgccaac tgattaaggt gcggattttt atgacaaaat
     1621 aaaaatcatc tttccgcagt cattcccacg gacgcgaaag tctgtctttt tgaatcccgg
     1681 ataattttac atcgtcattc ccgcgcaggc gggaatctag tctgttcggt ttcggtcatt
     1741 tacgataaat tcctattgct tttcatttcc tgattcccac ttccgtggga atgacggcgg
     1801 agcggataaa ttctcgcaat ctaaaacctc g
//
LOCUS       DAWFAD010000215         1800 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWFAD010000215 DAWFAD010000000
VERSION     DAWFAD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.215"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            97..525
                     /locus_tag="WGO49_001967"
     CDS             97..525
                     /locus_tag="WGO49_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0307019.1"
                     /translation="MGNFAKVSGYTYNKSRYRNAAEVNAERLAKNSSADPYNFSNFTP
                     VHIFRFGTSFHIPNTGLTVGGGVSAQSGTSSLYNIRQGGYGLIDGFVRYELGKHAKLS
                     LIGTNLNGRTYFENNYNRTRGANNFYGEPRTVSMKLDWQF"
     gene            669..1727
                     /gene="dinB"
                     /locus_tag="WGO49_001968"
     CDS             669..1727
                     /gene="dinB"
                     /locus_tag="WGO49_001968"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ0307020.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLIARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT          427 a          485 c          495 g          393 t
ORIGIN      
        1 cagtcgatgt gcttggcaat catggcttgg gcggtttgcc ggaagaaggt gctcatgaga
       61 aatcccctaa atgtcttggt ggaaatttag gggattttgg ggaattttgc aaaggtctca
      121 gggtatacct acaataagag caggtatagg aatgcagccg aagtcaacgc cgaacgcctt
      181 gccaaaaatt ccagtgcaga cccgtacaac ttcagcaatt tcacacccgt gcacatattc
      241 cgtttcggaa cgagcttcca tataccgaat acggggctga ccgtcggcgg cggcgtgtcc
      301 gcacaaagcg gcacaagcag cctgtacaac atccgtcagg gcggctacgg gctgatagac
      361 ggtttcgtcc gttacgaatt gggcaaacac gccaaattga gcctcatcgg tacgaactta
      421 aacggacgca cttattttga gaacaactac aaccgtacgc gcggcgcaaa caacttctat
      481 ggagagccgc gcactgtcag catgaaactg gattggcagt tttaattgaa aaccgtagtt
      541 ttgcggatgc cgtctgaaac agggtatgtt tcagacggca tttttatgga ggcaggccta
      601 tcggggcgta tatttggaat ttgcccgatg ccggcagtag aatatccctt ttatcctcag
      661 aagagccgat gtcttcacgc aaaattatcc acatcgacat ggacgcattc tacgcatcgg
      721 tagagctgcg cgaacagccg catttgaaag ggcggccggt ggtcgtcgcg tgggagggcg
      781 cgcgttcggt gatttgcgcc gcatcgtatg aggcacggca gttcgggctg cattccgcga
      841 tgtcggtggc aacggcgaaa aggctgtgtc cgcaagcggt gtatgtgccg ccgcatttcg
      901 atttgtaccg tcaggtttcc gcgcagattc acgccgtatt caggcgttat accgatttaa
      961 tcgaaccctt gtcgctggac gaagcctatc ttgacgttac ccgtaatttc aaaaacatcc
     1021 cttacgccag cgaagttgcc aaagaaatcc gtgccgccat ttttgcggaa acaggtttga
     1081 ctgcatccgc aggcatcgcg ccgaacaaat ttctggcgaa aatcgcgtcg gactggcgca
     1141 agccgaacgg gcagtttgtg ttgccgccgc acaaagtcat ggcatttttg gaaaccctgc
     1201 ctttgggcaa aatccccggc gtgggcaagg taacgctgaa aaaaatgcag tcgctgggta
     1261 tgcggacggc gggcgacttg cgccgtttcg agcgcggcga actcttaaac catttcggac
     1321 gctacggata ccgcctctat gatttggtgc gcggtacgga tgaacgcccc gtcaaagccg
     1381 aacgcgaacg cctccaaatc tccacagaaa tcaccctgcc tgaagacctg ccgctcgagc
     1441 aggctgccgg acacctgccc catcttgctg aagacctgtg gcggcaaatc acgcgcaaaa
     1501 acgtcgaagc ccaaagcgta acgctcaagc tgaagaccta cgatttccgc atcatcacgc
     1561 gcacactgac ttattcctcc gtattgcccg actgcgcagc tctgctgcag gctgcgcaaa
     1621 tgttgatagc gcgcgtcccg ccgcagacgg aagacgcgtt ccgccttatc ggtatcggcg
     1681 tggggcatct tgtgccgaaa aaccagcagc aggatttgtg ggcgtaaacc gctttacgcg
     1741 cgccgtccaa aaaacatgcc gtctgaacgg ttttcagacg gcatggctgc tttatagtgg
//
LOCUS       DAWFAD010000216         1755 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWFAD010000216 DAWFAD010000000
VERSION     DAWFAD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.216"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            106..1479
                     /locus_tag="WGO49_001969"
     CDS             106..1479
                     /locus_tag="WGO49_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0307021.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNISLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPKQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGSLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKTEMREPRQRNRYESARAQREKNTRPETTANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1659..>1755
                     /locus_tag="WGO49_001970"
     CDS             1659..>1755
                     /locus_tag="WGO49_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEZ0307022.1"
                     /translation="MSTQFDVAVIGAGPAGSVASALLRKKGYSGLN"
BASE COUNT          447 a          569 c          415 g          324 t
ORIGIN      
        1 ctgcggctcg ccgccttgtc ctgatttttg ttaatccact atatttcccc gtcctatcgg
       61 tttcccgttt cagacgacat aaggtctgaa agaaagacta caattatgag taatccattt
      121 tcctctttag gtttgggtac ggaactcgtt tccgcactga ccgcgcaagg ttacgaaaac
      181 ccgacgccca tccaagccgc cgccattccc aaagcactcg ccggtcatga tttgctagcc
      241 gccgcgcaaa ccggcacagg caaaaccgcc gcctttatgc tgcccagtct ggaacgcctc
      301 aaacgttacg ccaccgccag cacctcgccc gcgatgcacc ccgtgcgtat gctcgtcctt
      361 acccccacgc gcgaacttgc cgaccaaatc gaccaaaacg tgcagtccta catcaaaaac
      421 ctgccgcttc gccacaccgt cttgttcggc ggtatgaata tggacaaaca gaccgccgat
      481 ttgcgtgccg gctgcgaaat cgtcgtcgcc accgtcggac ggctgctcga ccacgtcaaa
      541 caaaaaaaca tcagtttgaa caaagtcgaa atcgtcgttc tggacgaagc agaccgcatg
      601 ttggatatgg ggtttatcga cgacatccgc aaaatcatgc agatgctgcc caaacaacgc
      661 caaaccctgc tcttttccgc taccttctcc gcaccgatac gcaaactggc gcaagacttc
      721 atgaacgcgc ccgaaaccgt cgaagtcgcc gcgcaaaaca ccaccaacgc caacgtcgaa
      781 caacacatca tcgccgtcga taccattcag aagcgcaacc tgctcgaacg gctgattgtc
      841 gatttgcata tgaaccaggt catcgtgttc tgcaaaacca aacaaagcgt cgaccgcgta
      901 acgcgcgaac tggtgcgccg caacctgtcc gcacaggcga tacacggcga ccgttcccaa
      961 caaagccggc tcgaaacact caacgccttc aaagacggca gcctgcgcgt cctcgtcgcc
     1021 accgacatcg ccgcgcgcgg gctggacatt gccgaactgc ccttcgtcat caattacgaa
     1081 atgcccgccc agcccgaaga ctacgtccac cgcatcgggc gcacggggcg cgcgggcgcg
     1141 gacggcgtgg cgatttccct gatggacgaa tccgaacaga aaatgtttga atccattaaa
     1201 gagctgaccg gcaacaagct gctcatcgag cgcatcgagg gcttcgagcc gcaatggtgg
     1261 gaacagggcg gcgcaaaacc ggaaaaaacc gaaatgcgcg aaccgagaca acgcaaccgc
     1321 tacgaatccg ccagggctca gcgcgaaaaa aatacccgac cggaaaccac ggcaaacgat
     1381 gcgggcgcgg cttgcggaaa aattgccgga cgcagccgcc gaagccgtcg ggaacaccgg
     1441 acgtgcgccc tgctccaacc gcgttacggc gtaaaataac cctgaaaatc aaatgccgtc
     1501 tgaacatttc ccgtttcaga cggcattttt caaaccggac tgacgcattg ggagcaaccg
     1561 cccgcaccgg ataaatttct tccgcaaaca gtttcagacg gcatttgctg cctgtacaat
     1621 atcatttcct tttccactca aaccatcagg atttcccaat gtccacccaa ttcgatgtcg
     1681 ccgtaatcgg cgcaggtccg gcaggctcgg tcgcatccgc cctgctccgc aaaaaaggtt
     1741 atagtggatt aaatt
//
LOCUS       DAWFAD010000217         1747 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWFAD010000217 DAWFAD010000000
VERSION     DAWFAD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.217"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            35..922
                     /gene="prmA"
                     /locus_tag="WGO49_001971"
     CDS             35..922
                     /gene="prmA"
                     /locus_tag="WGO49_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ0307023.1"
                     /translation="MPYQQITINVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEEGWARLSGTKR"
     gene            940..1503
                     /gene="orn"
                     /locus_tag="WGO49_001972"
     CDS             940..1503
                     /gene="orn"
                     /locus_tag="WGO49_001972"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ0307024.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESLHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPSVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
BASE COUNT          468 a          538 c          433 g          308 t
ORIGIN      
        1 aattcaatcc actatatttc caagaaagcc cgttatgccc taccaacaaa tcaccatcaa
       61 cgtcaacgat gccgtcgccg aacgcctcgc cgacgcgctg atggaacacg gcgcactctc
      121 cgccgccatc gaagatgcct acgccggcac gcaaaacgaa caggcgattt tcggcgaacc
      181 cggtatgccc gccgaacaaa tctggcagca gagcaaagtc atcgccctgt tcggcgaaca
      241 cgacgaagcc gccgccatca tccaaatcgc cgcacaagaa tgcgggttaa aagacttggc
      301 atacaccggc gaaaccatcg aagaccaaga ctgggtgcgt ctcacgcaat cgcaattcga
      361 ccccatccga atttccgacc gcctgtggat taccccctct tggcacgaag cccccgaagg
      421 cactgccgtc aacctacgcc tcgatcccgg cctcgccttc ggcactggca gccacccaac
      481 cacgcgcctc tgcctcaaat ggctggatac gcaactcaaa aacggcgaaa gcgtcctcga
      541 ctacggctgc ggttcgggca tcctgaccat cgctgccctc aaactcggcg caggttctgc
      601 cgtcggcgtc gatattgacg aacaagccgt ccgcgccggc aaggacaacg ccgaacaaaa
      661 caacgtcgat gcacaattct tcctgcccga cggcctgcct caagggcaat tcgacgtagt
      721 tgtcgccaac atcctcgcca accctttacg tatgctcggc gaaatgctcg ccgcccgcac
      781 caaacaaggc ggccgcatcg tgttatccgg tttgttggac gaacaagccg aagaactcgg
      841 cggcatttac agccaatggt tcgatctcga cccggcggaa accgaagaag gctgggcgcg
      901 cttgagcggg acaaaacgct gaaacggaaa ggaaacaccg tgcaggacaa aaacaacctc
      961 tgctggctcg atatggaaat gacggggctt aatcccgaaa ccgaccgcat tatcgaagtc
     1021 gcgatgatta ttaccgactc ggatttgaat gtattggcgc aatccgaagt ttacgccgtc
     1081 caccaaagcg acgagctgct cgacaatatg gacgaatgga acaccgccac acacggtagg
     1141 acggggctga cacagcgcgt gcgcgaatcg ttgcataccg aagccgaagt cgaacagaaa
     1201 ctgctggact ttatgtcgga atgggtaccc ggacgcgcca cgccgatgtg cggcaactcc
     1261 atccaccaag accggcgttt tatggtcaaa tatatgccga aactggaaaa ctacttccac
     1321 taccgcaacc tcgacgtttc cacgctcaaa gagcttgcca aacgctggaa tccgtccgtt
     1381 gccaaaagcg tcgtcaaacg cggttcgcac aaggcattgg acgacatttt ggagagcatc
     1441 gaagaaatgc gccactaccg cgaacacttt ctgatttccg ccccgagagc cgaagcgcaa
     1501 taagaaacaa aaaatgccgt ctgaagcgca aatcctgcat ttcagacggc atttttacag
     1561 cagattgaaa tcaaaaatat acacgcccgt cattcccgcg caggcgggaa tccggaaggt
     1621 cgggcctgcc gttattttca atcattacag aaactgaaag gtctggattc ccgcctgcgc
     1681 gggaatgacg ggcgtgtgca ttcttatagt ggattaacaa aaatcaggac aaggcgacga
     1741 agccgca
//
LOCUS       DAWFAD010000218         1747 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWFAD010000218 DAWFAD010000000
VERSION     DAWFAD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.218"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(96..758)
                     /locus_tag="WGO49_001973"
     CDS             complement(96..758)
                     /locus_tag="WGO49_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0307025.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPPKSMDEIRNQ
                     ASHGAAGLLKLGTGITPDHPDYARWRTEYLDEYDSRYAQDTTLFGGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLAPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(771..1598)
                     /locus_tag="WGO49_001974"
     CDS             complement(771..1598)
                     /locus_tag="WGO49_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ0307026.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNTPP
                     LDFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRAFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
BASE COUNT          338 a          456 c          535 g          418 t
ORIGIN      
        1 cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgacgta ctggtttaga
       61 tttaatccac tatatgtttg tggggcggat atttttcagg gctgtatttt gtccagacat
      121 tcgagcagat cgagcggcgt gcggatgtgg aaatccgcct gccatgagcc ggtatcgtct
      181 tcgggagcga tgtagcccca ttcggcgagg acggttttca tgccggcgtt gcggcctgct
      241 tggatgtcgc gttcggcatc gccgacgtag agtgtgtgtt gcgggtcggc gtggatttgt
      301 ccgcacgcat acagcatggg tttgatgctg ggcttgggtt cgccgcaggt gtcgccgctg
      361 acgacgacgg cgggtgggat gatgaagccg agtttggggg cgagtttgtc ggtgaagcgc
      421 atgggtttgt tggtgatgat gccccatttg atgccgcgtc tgtcgagttc ggcgatgagt
      481 tcgtttacgc cgccgaagag ggtggtgtct tgggcgtagc ggctgtcgta ttcgtcaagg
      541 tattcggtgc gccatcgggc atagtcgggg tggtcggggg tgatgcctgt gccgagcttg
      601 agcagtcctg ctgcgccgtg gctggcttgg ttgcggattt cgtccatgct tttgggcggc
      661 agtccgtggc gggcgagcag ggtattgagt gcgccgccga ggtctagggc ggtgtcggca
      721 agcgtgccgt cgaggtcgaa gagtacggcg tgtatcatgt atgttccttt tcagacggta
      781 tttttgttct gtttcaaacc ctgctcgaaa tcttccagca tatccaattc aaagcggctg
      841 aagcccgctt tttcgcgcgc ttcgatgttc acatagccgc ggaagataaa catatcgtaa
      901 cgggcaatca gactgcggaa cagggtaatc ggttccaaac cgcgttcgcg gcagaggtgt
      961 tgataccaat ggttgccgat ggcgacatga cccacttcgt cgcggtaaat gatgtccaat
     1021 acgccgcagg tttccgaatc gccgcgctgc gccaccttcg cgcgtatccc cggcgtaacg
     1081 tccagcccgc gcgcttccaa cacgcgcggt accaacgcca tacgcaacag cgggtcgtag
     1141 gcggttttgt acgccatatc ccacaaatgg ttgtgcgcct cgaaactgcc gtaatcgaag
     1201 ccgaaagcgc gcaatctgtc gcgcatcagg cggaaatggt acacctcttc cttcgccact
     1261 ttcacccagt cgcggacaaa ctgaaacggc agcgtgcgga aacggtatgc cgcgtccaaa
     1321 gctagattga cggcgttgaa ttcgatatgc gcaatcgcgt gcagcatcgc cgcatagcct
     1381 tctgccgtgt tcattttgcg cggggtcagt tgtgacggcg cgaccaaaac cggcttgtcc
     1441 ggccgccctg cgcgggggaa atccagcggc ggcgtgtttg tttcagcacc gtccatgttc
     1501 tgaacggcgg caaacgcctc atccgtcagc cgtccttttt catctgggtc gcccgaaagc
     1561 agggcgcgtt ccagcaaagc ataaatatcg agtttcatct caagtccgcc gtgttcggaa
     1621 aacaaatatt atagcgtttt aaaaaaacaa gatgaggcat ataatctccg cgattcggca
     1681 ttccgcgccc aaaccgtcaa atatagtgga ttaacaaaaa ccggtacggc gttgcctcgc
     1741 cttagct
//
LOCUS       DAWFAD010000219         1738 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWFAD010000219 DAWFAD010000000
VERSION     DAWFAD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.219"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..638)
                     /gene="rfbA"
                     /locus_tag="WGO49_001975"
     CDS             complement(<1..638)
                     /gene="rfbA"
                     /locus_tag="WGO49_001975"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0307027.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQIKPSARGELEITTLNQMYLDDGSLNV"
     gene            complement(701..>1738)
                     /gene="rfbB"
                     /locus_tag="WGO49_001976"
     CDS             complement(701..>1738)
                     /gene="rfbB"
                     /locus_tag="WGO49_001976"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0307028.1"
                     /translation="GFIGSAVVRHIIRNTQDSVVNLDKLTYAGNLESLTDIADNPRYA
                     FEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAA
                     RAYWQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPYSASKVSSDHL
                     VRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLF
                     VEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLEELAPEKPAGVARYED
                     LITFVQDRPGHDVRYAVDAAKIRRDLGWLPLETFESGLRKTVQWYLDNKTWWQNVLNG
                     SYRLERLGTGK"
BASE COUNT          389 a          422 c          502 g          425 t
ORIGIN      
        1 tggacattga gcgaaccatc gtccaaatac atttgattca aggtggtaat ttccaattcg
       61 ccgcgtgcgg acggtttgat ttgtttggcg aactcgacgg cacggttgtc gtagaagtac
      121 aagccggtta ccgcccaatc ggatttgggc cgttgcggtt tttcttcgat ggaaacggcg
      181 cggaagtttt cgttaaattc aaccacgccg aaacgttcgg ggtttttgac ctgataagca
      241 aacacggttg cgccgtgcgt ttgcgctgcc gcctgtttca atgtttgcgt aaacgactga
      301 ccgtaaaaaa tattgtcgcc caaaaccaag caaacattgt cgttgccgat aaattcttcg
      361 ccgatgataa atgcctgtgc caagccgtcc ggactgggtt gcacggcata actgatggaa
      421 atgccgaaat cgctgccgtc gccaagcagg cgtttgaaag aggcgttgtc ttcaggcgcg
      481 gtaatcacca aaatatcgcg gattcccgcc agcatcaaaa ccgacaaggg gtaataaatc
      541 atcggtttgt cgtacacggg caggagctgt ttggatacgc cgcgcgtgat ggggtagagg
      601 cgcgtgccgc tgccgcctgc cagtatgatg cctttcatct ttgttttctt cctttgcgat
      661 gggttttcag acggcattgc gtcgggatgc cgtctgaaaa ctattttcca gtacctaaac
      721 gttccaaacg atagctgccg ttcaatacat tttgccacca ggttttgttg tccagatacc
      781 attgcacggt tttgcggagg ccggactcga aggtttccaa aggcagccag cccaaatccc
      841 gcctgatttt ggctgcgtcg acggcgtagc gtacgtcatg gccggggcgg tcttgtacga
      901 aagtaatcaa atcttcataa cgcgccacac cggccggttt ttcgggagcg agttcttcca
      961 gcagggcgca gatggttttg acgacttcaa tattggcttt ttcattgtgg ccgccgatat
     1021 tgtaggtttc gccgacaaca ccttcggtaa caacctgata cagtgcgcgc gcgtggtctt
     1081 cgacaaacag ccagtcgcgg atttgcatac cgtcgccgta cacaggcagc ggtttgccgt
     1141 caagcgcgtt cagaatcatc aaaggaatga gtttttccgg aaaatggtaa ggaccgtagt
     1201 tgttggagca gttggttaca atggtcggca agccgtaagt acgcaaccac gcgcggacga
     1261 ggtggtcgct ggacacttta gaggcagagt aggggctgga cggcgcgtag ggcgcggttt
     1321 cggtaaacaa atcgtccgtg ccgcctaaat cgccatagac ttcatcggtg gaaatatggt
     1381 ggaaacggaa ggcttcgtgc tgttcagacg gcatttgttg ccagtaggcg cgggctgctt
     1441 caagcagatt gaatgtgccg acgatattgg tttggataaa ctcgcctgcc gaaccgatag
     1501 agcggtcgac atggctttcc gccgccaagt gcatcacggc atcgggccgg tgttgtgcga
     1561 atacgcggtc gagttcggcg cggtcgcaaa tgtccacttg ctcgaaagca tagcggggat
     1621 tatcggcaat gtcggtcagc gattcgagat tgcccgcgta ggtcagtttg tcgaggttga
     1681 cgacggaatc ttgggtgttt cggataatat gacggacaac ggcagaaccg ataaagcc
//
LOCUS       DAWFAD010000220         1735 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWFAD010000220 DAWFAD010000000
VERSION     DAWFAD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.220"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            356..1660
                     /locus_tag="WGO49_001977"
     CDS             356..1660
                     /locus_tag="WGO49_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ0307029.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          356 a          433 c          513 g          433 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cggcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggga tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggcatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggagcgcatt gggtgtgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtcg ggcgcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggcgaacg cgtggaataa gggcggttat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga taaac
//
LOCUS       DAWFAD010000221         1735 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWFAD010000221 DAWFAD010000000
VERSION     DAWFAD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.221"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(114..770)
                     /locus_tag="WGO49_001978"
     CDS             complement(114..770)
                     /locus_tag="WGO49_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ0307030.1"
                     /translation="MKNKSKVWDFPTRLFHWLLAASLPFMWYSAKTGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIREYMKNGIPEHVQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALVAAAILLLS
                     "
     gene            835..1725
                     /locus_tag="WGO49_001979"
     CDS             835..1725
                     /locus_tag="WGO49_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ0307031.1"
                     /translation="MFPVFHLSGESRRRMLQAASRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMRGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWMVGCGLGTDKAAVGTLAGILAEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGVWLHGAAADVIKESAGIAAGL
                     LAGEISPAARWLRNRITESM"
BASE COUNT          384 a          413 c          577 g          361 t
ORIGIN      
        1 ggtttgggac ggaaaaacgg cctgaagccg tttcgggcat tcagaccgtt tgcgcggaga
       61 ggggatgccg tccgaagggc gaaaagggct tcagacggca ttgatgtcgg gtttcaggac
      121 aggagcagga tggcggctgc gacaagcgcg gcaaccgata atgcggcggc aagcgcggtt
      181 ttgccggcaa agcggattga ggttttgcct tcgatgtatt tgaagccggt tatcatcggg
      241 aggacgaggt ttttcttttt gaacacgcgg tatgcggcga cggcggcgat atgaatggcg
      301 gaaaaaacgg cgagcagctt gaaaaagttg aggtggattt tccgcataag gctgcccgta
      361 tgctcggaaa ccaaatggtt gaggtagccg ttggtgctga aggtgttttc atcggcggca
      421 aaaagccctg tgccgacttg aaatgagacg gcggccaaaa gcgcgacgac catcagtgcg
      481 cccaaggggt tgtgtccggg ttggacgtgt tcgggaatac cgtttttcat atactctctg
      541 atacccgacc atccgcggac gaaacgggag aaacgggcgg tatcgctgcc ccaaatgccc
      601 cagcagaggc ggaatacgag caggaaaagg ataaacagcc cgacgcgcgt gtgccattgc
      661 agcatatcgc cgccggtttt cgcgctatac cacataaagg gtagggatgc ggcaagcagc
      721 cagtggaaaa ggcgggtggg gaagtcccag actttgcttt tgtttttcat aatcggtttc
      781 cggcggtaga atcggtttgt tttcgagcct taattttaac cgattggagg ggaaatgttt
      841 cccgttttcc atctttcagg cgaaagccgc cgccggatgc ttcaggcggc atcgcgtttt
      901 ccctatgttt tcaaagcccg tgcggaagat tcgcacaaag ggactttcgg cacgctcgcc
      961 gtagtcggcg gatcggcagg gatgagaggc gcgcccgtat tggcggcatc ggcggcgatg
     1021 tatctcggct gcggcaaggt gtgggcgggt ttcaatcagg atgcgcttcc gtttgccgtc
     1081 attgccggtt ttcccgaaat tatgttggac acggcagacg gtttgaccaa gcgtcaaggc
     1141 ataaacgctt ggatggtagg ttgcggcctg ggtacggata aagcggcggt cggaacgctt
     1201 gccggaattt tggcggaaca cacggacaag cccgtccttt tggatgcgga cgcgctgaac
     1261 atattatcaa ccgatgccga aacccgaaat ctggcgcgcg ggtgtaaaaa cctgatttta
     1321 acgccacacc ccgccgaagc cgcgcgcctg cttggaacga cggttgcaca ggttcaggcg
     1381 gatcggacgg cggcagtgag gaagataagg gcaattttcg gcgcaaccgt ggttttaaag
     1441 gggcacaaaa cattggttgc ctcacccgat acggaaatct atgtcaacga aagcggcaac
     1501 gcgggattgg caacggcggg cagtggcgac gtattgggcg gcatcatcgg cagtctgctc
     1561 gcacagggcg tgccggtttt tgaagccgcc tgcgcgggcg tgtggctgca cggcgcggcg
     1621 gcggatgtca taaaagaatc ggcaggcatt gcggcggggc tgttggctgg cgaaatctcc
     1681 ccggcggcaa ggtggctgcg caaccggata actgaaagta tgtaagaaga tatag
//
LOCUS       DAWFAD010000222         1729 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWFAD010000222 DAWFAD010000000
VERSION     DAWFAD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.222"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WGO49_001980"
     CDS             complement(47..1330)
                     /gene="hemL"
                     /locus_tag="WGO49_001980"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ0307032.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAMREAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPSEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FRTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIDAFKRFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1394..1567)
                     /locus_tag="WGO49_001981"
     CDS             complement(1394..1567)
                     /locus_tag="WGO49_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0307033.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
BASE COUNT          387 a          485 c          472 g          385 t
ORIGIN      
        1 caatctgcca aacagatcga acagagaaac tctgtccgtc aaaacatcat gcagccatcg
       61 ccttgaacac ttcaaccgca accgcaaccg tttcgtcaat cagctcgggc gtatgcgcgg
      121 cagaaacgaa acctgcttca taagcggacg ggccgaaggc aatgccgcgg tcgagcatac
      181 cgtggaagaa gcgtttgaaa gcgtcaatat tggaacgcgc catatcggca taatttcgcg
      241 gcgcgtgtgc ggcgaaatac agaccgaaca taccgcccac gctgtcggcg gtgaactcga
      301 tgcccgccgc atccgctgcc gtccgaaaac cttgaaccaa ctgttcggta cgcgccgtca
      361 ggttttcata aaacccttcg cggcggatga tttccagcgt tttcaaaccg gcggcgacgg
      421 caatcgggtt gcccgacaag gtgcctgcct gatacacgcc gcccagcggg gaaatacatt
      481 ccataatgtc tttgcgtccg ccgaacgcgg caagtggcat accgccgccg atgactttgc
      541 ccatcgtggt caggtcgggc gtgatgccgt gcagcgattg tgcgccgccg agcgcgacgc
      601 ggaaaccggt catcacttcg tcgtaaatca acactgcgcc gtgtttttcg gtcaattcgc
      661 gcagggcttt gacgaaggct tcagacggcc taaccagatt catattgccg acaaaaggct
      721 ccaaaatcac acaggcaatg tcattgccgc tttgagcaaa ggcttcttcg agttgggcga
      781 tgttgttgta ttcgaggact aaagtgtgtt tggtaaagtc ggcaggcaca ccggcggaag
      841 agggattacc gaaggtgagc agaccgctgc ctgctttcac caacaggctg tcggaatggc
      901 cgtggtagca gccttcaaat ttgatgattt tgtcacggcc ggtaaagccg cgtgccagac
      961 ggatggcggt catggtggct tcggtaccgg agctgacaag gcgcagccgt tcgacggacg
     1021 gcatgatttt ggcgatttct tcggcaatga cgatttcgcc ttcggtaggc gcgccgaacg
     1081 acaaaccgcc caaggcagct tcgcgcatgg cttcgatgac ttcggggtgc gcgtgtccga
     1141 caatcgcggg cccccacgaa ccgacatagt cggtgtagcg cgtgccgttt tcgtcccaaa
     1201 catacgcgcc ttcggctttt ttgatgaagc gcggcacgcc gccgacgctg ccgaatgcgc
     1261 ggacggggga attcacgccg ccggggatga tggctttggc gcggtcgaat aaaatttcgt
     1321 tacggttcat atttatcctc aaatgccgtc tgaacggcag gtttcgggct tagaagcaga
     1381 aagtcccatt ttatcatttt tcaggttgcg acaaggattt gcctgcttct ttgcggatga
     1441 cgccaaccgc atcccggatg acggaacgct cgtctttttc cactttatgt gtaaagcggt
     1501 agtcccggac aactccttcc cagtcgtaat ccacacacca ctcccaatgc cggcgttctg
     1561 atttcatata aatgaaattg gtcggcaaaa aattataaat cggcaggctg acttcatgat
     1621 aggcataaca accgaaaggg ttgcgcttcc cgaaacgtgc ctctacctcc gcccgagtcg
     1681 ttttgccttt aacaaccgtt tgtgcgattc cctcttccgt ctgatatag
//
LOCUS       DAWFAD010000223         1706 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWFAD010000223 DAWFAD010000000
VERSION     DAWFAD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.223"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            180..509
                     /locus_tag="WGO49_001982"
     CDS             180..509
                     /locus_tag="WGO49_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0307034.1"
                     /translation="MGSLIIEDLQESFGKEAVKGKEITVHYTGWLEDGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            538..900
                     /locus_tag="WGO49_001983"
     CDS             538..900
                     /locus_tag="WGO49_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0307035.1"
                     /translation="MASSNVNKEIKDKKLSLWAKRQDGSVKWFCGQPVTRNDTAKDDT
                     VAADTDTAKKIDTKHLPSTCRDDSSTGCIETPRADFKHFQKISRYRVLPESRHMAEKL
                     RHSRAGGNLGLSARKLIG"
     gene            947..1240
                     /locus_tag="WGO49_001984"
     CDS             947..1240
                     /locus_tag="WGO49_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0307036.1"
                     /translation="MTGFNDAAGVASASDIKGKYVEKVEVKNGVVTAEMKSSGVNKEI
                     QGKKLSLWAKRQDGSVKWFCGQPVARNDKADTDKIDTKHLPRRSICRLRQIIP"
BASE COUNT          500 a          442 c          416 g          348 t
ORIGIN      
        1 aaatttaatc cactatattt ccatgaaaaa gcatggaacc tttacgaaaa aaataaaaca
       61 gtcaaacaaa cacagccgtt tcagctgcac cggagcagct gacgtttgag cggcatttga
      121 cttcgccgga cggtatgtta cccttgccgt tttaaccaac aagcaacgaa aggacaaata
      181 tgggcagcct gattattgaa gatttgcagg aaagcttcgg aaaagaagca gttaaaggca
      241 aagagattac cgtgcattac acaggttggc tggaagacgg caccaaattc gactccagcc
      301 tcgaccgccg ccagccgctg accatcacgc tcggcgtcgg acaagtcatc aaaggctggg
      361 acgaaggctt cggcggaatg aaggagggcg gcaaacgcaa gctgaccatc ccttcggaaa
      421 tgggctacgg cgcacacggc gccggcggcg tgattccccc gcacgccact ttgatatttg
      481 aagtcgagct gctgaaagtg tacgaataaa gctgcctggg caataccgtc tgacataatg
      541 gcttcaagca acgtaaacaa agaaatcaaa gacaaaaaac tctccctgtg ggccaagcgt
      601 caagacggtt cggtaaaatg gttctgcgga cagccggtta cgcgcaacga caccgccaaa
      661 gacgacaccg tcgccgccga caccgacacc gccaagaaga tcgacaccaa gcacctgccg
      721 tcaacctgcc gcgatgattc atctaccggt tgcatagaaa caccacgcgc cgacttcaaa
      781 cacttccaaa aaatcagccg ttaccgagta ttgcccgaat cacggcacat ggccgaaaaa
      841 cttcgtcatt cccgcgcagg cgggaatcta ggtctgtcgg cacggaaact tatcgggtaa
      901 aacggtttct tgagattttg cgttctagat tcccactttc gtgggaatga cgggatttaa
      961 tgatgccgcc ggcgtggcat ccgcctccga catcaaaggc aaatatgttg agaaagttga
     1021 agtcaaaaac ggcgtcgtta ccgccgaaat gaaatcaagc ggcgtaaaca aagaaatcca
     1081 aggcaaaaaa ctctccctgt gggccaagcg tcaagacggt tcggtaaaat ggttctgcgg
     1141 acagccggtt gcgcgcaacg acaaagccga caccgacaaa atcgacacca agcacctgcc
     1201 gcgacgcagc atctgccgat taaggcaaat tataccataa attttaaata aatcaagcgg
     1261 taaatgattt tccacggcca ctcggattaa tccgggtggc ttccttttta aaggtttgca
     1321 aggcaagcgg ggtcgtccgt tttggtggaa ataatatatc gattgcgctt caaggctctg
     1381 cgcgtacctc attgccgccc gtttaaacac ggggtttatc tgacaggcgc gtaatccgcc
     1441 ctctcattta ccgaacaagc gaactggatt cccgcctact cgggaatgac gaagctatcc
     1501 gtacggaaac ctgcaccgcg tcattcccac gaaagtggga atccagtttt ttgagtttca
     1561 gtcatttccg ataaattgcc ttagtattga atgtctagat tcccgcctgc gcgggaatga
     1621 cggcaaccgg ccggttacgt atcaaaaaaa taaagtaatt cggctagata tagtggatta
     1681 acaaaaatca ggacaaggcg acgaag
//
LOCUS       DAWFAD010000224         1646 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWFAD010000224 DAWFAD010000000
VERSION     DAWFAD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.224"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            295..1452
                     /locus_tag="WGO49_001985"
     CDS             295..1452
                     /locus_tag="WGO49_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0307037.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     QNIQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGATQWG
                     NRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMPVSVR
                     YDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQQTPKSQPTLVPAVVGKPGSDVYYAGL
                     NYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTGGYEE
                     GGLNLALAAQLDLSENGDKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKGE
                     NTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          413 a          393 c          422 g          418 t
ORIGIN      
        1 tgccgcgtgt gtttttttat ggcgttttaa aaagccggga ctgcatccgg gcagcagcgc
       61 atcggcccgc acgaggtctg cgcttgaatt gtgttgtaga aacacaacgg tttttgaaaa
      121 aataagctat tgttttatat caaaatataa tcatttttaa aataaaggtt gcggcattta
      181 tcagatattt gttctgaaaa atggtttttt gcggaggggg ggtataattg aagacgtatc
      241 gggtgtttgc ccgatgtttt taggttttta tcaaatttac aaaaggaagc cgatatgcga
      301 aaaaaactta ccgccctcgt attgtccgca ctgccgcttg cggccgttgc cgatgtcagc
      361 ctgtacggcg aaatcaaagc cggcgtggaa ggcaggaaca tccagctgca gttgaccgaa
      421 ccgctccaaa atattcaacc tcaggttact aagcgcaaaa gccgcatcag gacgaaaatc
      481 agcgatttcg gctcgtttat cggctttaag gggagcgagg atttgggcga agggctgaag
      541 gctgtttggc agcttgagca agacgtatcc gttgccggcg gcggcgcgac ccagtggggc
      601 aacagggaat cctttatcgg cttggcaggc gaattcggca cgctgcgcgc cggtcgcgtt
      661 gcgaatcagt ttgacgatgc cagccaagcc attgatcctt gggacagcaa taatgatgtg
      721 gcttcgcaat tgggtatttt caaacgccac gacgatatgc cggtttccgt acgctacgat
      781 tctccggaat tttccggttt cagcggcagc gtccaattcg ttccggccca aaacagcaaa
      841 tccgcctata caccggctca ttttgttcag cagactccta aaagtcagcc tactctcgtt
      901 ccggctgttg tcggcaagcc ggggtcggat gtgtattatg ccggtctgaa ttacaaaaat
      961 ggcggttttg ccgggaacta tgcctttaaa tacgcgaaac acgccaatgt gggccgtgat
     1021 gcttttgagt tgttcttgct cggcagcggg agtgatgaag ccaaaggtac cgatcccttg
     1081 aaaaaccatc aggtacaccg cctgacgggc ggctatgagg aaggcggctt gaatctcgcc
     1141 ttggcggctc agttggattt gtctgaaaat ggcgacaaaa ccaaaaacag tacgaccgaa
     1201 attgccgcca ctgcttccta ccgcttcggt aatgcagttc cacgcatcag ctatgcccat
     1261 ggtttcgact ttatcgaacg cggtaaaaaa ggcgaaaata ccagctacga tcaaatcatc
     1321 gccggcgttg attatgattt ttccaaacgc acttccgcca tcgtgtctgg cgcttggctg
     1381 aaacgcaata ccggcatcgg caactacact caaattaatg ccgcctccgt cggtttgcgc
     1441 cacaaattct aaatagcggg gcggtgaagc ggatagcttt gtttttgacg gcttgccttc
     1501 attctttgat tgcaatctga ctgccaatct gcttcagccc caaacaaaaa tccggatacg
     1561 gaagaaaaac ggcaataaag acagcaaata ccgtctgaaa gattttcaga cggtatttcg
     1621 cattttcggc ttggtttgca catata
//
LOCUS       DAWFAD010000225         1613 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWFAD010000225 DAWFAD010000000
VERSION     DAWFAD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.225"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            281..652
                     /locus_tag="WGO49_001986"
     CDS             281..652
                     /locus_tag="WGO49_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307038.1"
                     /translation="MNITLGRNHQINCDEKDLYKFIGYLANHPNDVNLVFEKNSVQGA
                     WGDEGRIQFFSSKAQNIFVPLGFKFTAGVGNIAYRLNCNELFEMLSQLDFVSGGKQNL
                     STIKANIPSQFHAEFDAGANM"
     gene            complement(716..1606)
                     /locus_tag="WGO49_001987"
     CDS             complement(716..1606)
                     /locus_tag="WGO49_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0307039.1"
                     /translation="MLSYCGVLSEEKFSRYYRYGVQNNKILQYIANRERNALNFIQAF
                     SEKLLKDSGIYPKFADFFAQPNKNTFESMKTAFTDLVIQNTPKNTEVEVRRIFTKIIN
                     PLAYKHNTFGTRKGSISNTPITLDELYYNRLNWRDKGKEKSLTRKEAQTLFADSANAA
                     NLNYLVNKATKFVKTLHKTSEVQRFDPTEANQAHHIFMASEFPDLASLPENLICLTPN
                     QHFNLAHPSNKTTVIDKHYQRICLMAKLDSIEQDNRANTGNYDYHEFIHVLNTGFNTD
                     QFDVSMSYETLKHRILMFDF"
BASE COUNT          496 a          238 c          326 g          553 t
ORIGIN      
        1 gggaagatta agggggtatt tgggtaaaat taggcggtat ttggggcgaa aacagccgaa
       61 aacctgtgtt ggggtttcgg ctgtcagcga aaaggtgaag agaggtactt tgcaaaggtc
      121 tcaacctgaa tatgtcaacc aaatcagcga caagctcgga gaattttttg gccggacgtt
      181 gaaataggag tttcaattct tttaattcag caatagatac tgataaaatg aaaatcttga
      241 ggaatatatc attaaaatta atttgttaag gagataatta atgaatatta cattaggtcg
      301 aaatcatcaa atcaattgtg atgaaaagga cctttacaag tttatcggct atttggcaaa
      361 tcatcctaat gatgtaaatt tagtctttga gaaaaatagt gtgcaaggtg cttggggtga
      421 tgagggacgt atacagtttt tctcaagtaa agcacaaaat atttttgtgc cactaggatt
      481 taaatttacg gcaggcgtag gtaatattgc atataggcta aattgtaatg agctttttga
      541 aatgctatct cagttagatt ttgtttctgg tggaaaacaa aatctttcaa caataaaagc
      601 aaatattccg tctcaatttc atgcagaatt tgatgctggt gcgaatatgt aaattctaat
      661 ttaaaacacg aatccgcttc atgagattcg tgttttttat ttttaaaata tttatttaaa
      721 aatcaaacat taaaatacga tgcttcaatg tttcgtaact catagaaaca tcaaactgat
      781 ctgtattaaa tcctgtattg agtacatgaa taaattcatg gtaatcgtaa tttcctgtat
      841 tagcacggtt atcttgctca atactgtcca atttcgccat caggcaaata cgctggtaat
      901 gtttgtcaat aacggtggtt ttattgcttg ggtgtgctaa attgaaatgc tggtttggtg
      961 tcaggcaaat cagattttca ggcagcgatg ctaaatcagg aaattcgctt gccataaaaa
     1021 tatgatgtgc ctgattggct tctgtgggat caaaacgttg aacttcgctg gttttgtgta
     1081 gcgttttgac aaattttgtg gctttattaa ctaaataatt caaatttgcg gcatttgcac
     1141 tatcggcaaa tagagtttga gcttctttgc gagtgagcga tttttctttg cctttatctc
     1201 gccagtttag gcgattgtaa taaagctcgt ctaatgtaat gggggtattg gaaatactcc
     1261 cttttcttgt accaaaagta ttatgtttat aagccaatgg attaatgatt ttggtaaaaa
     1321 tacggcgaac ttctacttcg gtatttttgg gtgtgttttg gataacaaga tcggtaaacg
     1381 ccgttttcat actttcaaac gtatttttat taggctgggc aaaaaaatcg gcaaattttg
     1441 gataaatacc actgtctttt aataattttt cactaaatgc ttggatgaaa tttagggcat
     1501 ttctttcacg attggcaata tattgcagga ttttattatt ctgtacgccg taccgatagt
     1561 atctagaaaa cttttcttcg cttaatacgc cacaatagct cagcatttta atc
//
LOCUS       DAWFAD010000226         1574 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWFAD010000226 DAWFAD010000000
VERSION     DAWFAD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.226"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            41..139
                     /locus_tag="WGO49_001988"
     CDS             41..139
                     /locus_tag="WGO49_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ0307040.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(210..1529)
                     /locus_tag="WGO49_001989"
                     /pseudo
     CDS             complement(210..1529)
                     /locus_tag="WGO49_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239425.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
BASE COUNT          288 a          430 c          507 g          349 t
ORIGIN      
        1 tcttgccgga tgctgcaaac aacgccggtt tgcattcctg atggcggtgg ttttcttaga
       61 cgaacgcccg aacacgcagg aatggatagg cttggggctg gttacggcgg gcgtgttgac
      121 gctggcactg aaacggtaaa gccgcaagaa ataaatgaaa tgccgtctaa aaaactgttt
      181 tcagacggca ttttcgtttc tgtccatcct cagcactcga ccacgcgcac ggatacgggg
      241 acggcttttt tccggagcgt ggcggcgagt ccggcaagcg aggtttcttt gtaggtcgcc
      301 ttcatatccc ggccggtttg cagcatggtt cggatgactt cgtcgagcga gacttttttg
      361 tccgtgccgt cttccaaaag cgcgagcgtg ccgagtttga gggctttttc ggcggcgatg
      421 ccgttgcgct cgatgcaggg gatttgcacc agtccgccga cggggtcgca agtcagcccc
      481 aaatggtgtt ccatcgccat ttcggcggcg ttttccactt gtttgggcgt gccgccgatg
      541 acttcggcgt atgcgcccgc cgccatcgaa cacgctacgc cgacttcgcc ctgacagccg
      601 acatccgcac cggaaatgga ggcgttggtc ttgtagagga tgccgattgc gcctgcggtg
      661 agcaggaagt tttcgacgcg ttcctgtgtg gcgtgcggat tgaacttgcg gaaatagtgc
      721 aatacggcgg gaatgatgcc tgccgcgccg ttggtcggtg cggtaacgac gcgtccgccg
      781 gcggcgtttt cttcgttgac cgccatggcg tacaccatcg gccagagctg ggtgttgacg
      841 atttcggttt cgcgcaggac tttgagcttg gcggcaagct gcggggcgcg gcggcggacg
      901 ttcaatcagc cgggcagttc gccgtccgca cccaagccgc gtttgatgca gccttccata
      961 acctcggcaa cggcagcggc gcggcggcgg atttcggctt cgccgcatcc ggcaagcgcg
     1021 gcttcgtttg ccaacacgac ttcggagatg tcgagccggt tcagacggca tcgggcaagc
     1081 agttcggcgc aactggtata gggataggga acggcttttt ccgtttccgc ctgccggtca
     1141 aaatcttctt cggtaacgac aaagccgccg ccgaccgaat aataaacctg ttcattcaat
     1201 accgtgccgt ctgaagcata ggcggtaaaa cgcaggctgt tggggtgttt gggcagcact
     1261 tgattgccga gtatgttcag gtcgcggtcg gggatgaagc ggatttcttg cccgttgagc
     1321 cggaggatgt gctgcgtgcg gatgcgttcg aggcgttcgg gaatgccggc aagcgggatg
     1381 tcgtgcggca ggctgccttc caaaccgagc atcagcgcgt caaatgtacc gtgtccgtat
     1441 ccggtcagtg cgagcgagcc gtaaatgtcg atgacgatgc gaacagcctg tgcatccaaa
     1501 cctgccgcaa aggcggcggc tgccttcatc gggccgaccg tatgcgaact ggaaggcccg
     1561 ataccgattt tgaa
//
LOCUS       DAWFAD010000227         1531 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWFAD010000227 DAWFAD010000000
VERSION     DAWFAD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.227"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..435
                     /locus_tag="WGO49_001990"
     CDS             <1..435
                     /locus_tag="WGO49_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0307041.1"
                     /translation="IIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVAKID
                     AIRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKEL
                     SDIGVLQEIAIGRDKLFIHPRLMELLRGEGNSFTSFQSLVKA"
     gene            465..1343
                     /locus_tag="WGO49_001991"
     CDS             465..1343
                     /locus_tag="WGO49_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0307042.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLLVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEALDLPEGSVILGVVLEVKKRF
                     MSAAQARRRVDSDYRP"
     gene            1347..>1531
                     /locus_tag="WGO49_001992"
     CDS             1347..>1531
                     /locus_tag="WGO49_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ0307043.1"
                     /translation="MDGWYDFRFQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAK
                     SEQRLSALSEWVGQLET"
BASE COUNT          404 a          395 c          411 g          321 t
ORIGIN      
        1 attatcgaaa acagggcgga ctattaccgc ctgcttttag gcgtaaccga acggcaggac
       61 tgggaaagct ggataatcta catcttagac ggcgtagccg acaccgccga ttggacggta
      121 gcgaaaatag atgcgatacg ccgcctgttt gagcagacac gtcaacacat acggacacac
      181 gcacaaggaa tctacacgca cgaactggta aatcttctgt ttgagcagcc atatacacgc
      241 attgccaacc tagaagcggc agggatagcc aaacggcaga cggcctctaa gtacctgaaa
      301 gagctttcag acataggtgt gctgcaagaa atcgccatcg gcagggacaa actattcatt
      361 catccgcgcc taatggaact attgcgggga gagggcaaca gctttacctc attccaatcc
      421 ctcgttaaag catagccaaa ataatcaata atccggaggt caatatggca agaaggtcaa
      481 aaacatttga agaagctgct gctgaggttg aggaacgttt cggtcatcgt ggcattaagt
      541 tggtcgagtt tgagggtaca gccaagccgt gtgtaatcaa ctgccctaaa catggaaacc
      601 aaacctgttc gaggtactcc aatatgttca taggaagtag ctggggttgc ccctcttgtg
      661 gtaatgagca agctgcaaaa gccggtatag cgacccttag gaagaatcac atagcgttag
      721 aaatgctgaa acaggctgta acaggtatga ccaagcaaga gcgcatcacg acgcaagcct
      781 acaatgagat gaccaaatcc gtggcaggtt caaacagcat agtccttaac gatgtccaag
      841 gcgatacgac catcaacaac catcatacgc atacgcacaa ccacagcgat gccgacggca
      901 aagcactgtc gatgaggctc acaccacgcc ccctgttgtc agaccgtcag gcggcggctt
      961 tcgcccgtac aggcaaactc acaggcagtt tcgacttgtt tgcttcggtg gtcgccccct
     1021 cgcagtacac gtttgccgtt gccatgcccg acacgtccat gtcgccggtt atcgaaaagg
     1081 gggatttgct ggtggtcgag ccgcgtatgc gccctgcgga cgaagacatc gtactgattg
     1141 aactgtccga caagcggctg gtcgtcgcgc accttgttat cgatattgcg ggcaggatgc
     1201 tgatttatca gacgggcagg ccgtctgaag ccctcgacct gcccgaaggc agcgtgattt
     1261 taggtgtggt gctggaggtc aaaaaacggt ttatgtccgc cgcacaggca agaaggcgtg
     1321 ttgattcgga ttaccgcccc tgatgtgtgg acggttggta cgatttccgc ttccaaaacg
     1381 tcgtgtacgc gcccgaccgc agcccggaaa tcagccgtat gctttcttcg attttggcag
     1441 gctacgcgtg ggataccgaa aacccgttcg tggcgaaatc cgaacaacgc ctgtcggcct
     1501 tgtcagaatg ggtcggtcag ttggaaaccg a
//
LOCUS       DAWFAD010000228         1506 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWFAD010000228 DAWFAD010000000
VERSION     DAWFAD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.228"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            27..1364
                     /locus_tag="WGO49_001993"
     CDS             27..1364
                     /locus_tag="WGO49_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0307044.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGQNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGIWGEFKVFGKTIFDLWDYVISAVIMPVGALGVA
                     VFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFVNSLDIL"
BASE COUNT          285 a          358 c          406 g          457 t
ORIGIN      
        1 tatcttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtgct tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
      121 atacggcagg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
      181 tggtcgccct gcccgtccag cttgccgagt tttatatcgg gcgcacaggc ggtcaaaacg
      241 ccgtcgattc cttcagggct ctgcgtccga gtacgcaatg gctttgggtc gggcgcatgg
      301 gcgtttccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattaa
      361 attatgtcgt ccacagtttc acgggggcgg ttcataccgg cgcggacttt gaagccttgt
      421 tcggcgcgac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtctg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggc caggcgtttt ttgccttgag catcggcgtt tccgctatga
      721 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg ccggcagtgt
      901 ttatgaagat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcgttt tcgatgttgg aaacggtcat tgcctcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa cacacttggc ttatcggcac ggctattttc attgtcggca
     1081 tcccgtccgc gctgtctttc ggcatctggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactatgtt atttccgccg tcattatgcc tgtcggcgcg ttgggcgttg
     1201 ccgtatttac cgcctggatt caggacaagc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ctgctgctgt ggctgaatac cttgcgctac ctcgccccga
     1321 ttgccattat tattgttttc gtcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 tcaaagtata gtggattaac aaaaatcagg acaaggcgac gaagccgcag acagtacaaa
     1501 tagtac
//
LOCUS       DAWFAD010000229         1425 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWFAD010000229 DAWFAD010000000
VERSION     DAWFAD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.229"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..372
                     /locus_tag="WGO49_001994"
     CDS             <1..372
                     /locus_tag="WGO49_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEZ0307045.1"
                     /translation="RLRRLVLIFVNPLYIIATTVPAQQAFLDAAELMQWSIETEGLGL
                     NVISHKILGKDHAQVEFEAYFQDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQP
                     CICGSGKKFKACCGKYLQPVA"
     gene            375..556
                     /locus_tag="WGO49_001995"
                     /pseudo
     CDS             375..556
                     /locus_tag="WGO49_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
     gene            complement(590..1384)
                     /locus_tag="WGO49_001996"
     CDS             complement(590..1384)
                     /locus_tag="WGO49_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ0307046.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDEFWSGQHVFKRSVIAALEPNLFERWVRWIRENQ"
BASE COUNT          382 a          425 c          300 g          318 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat acatcatcgc tactaccgtt
       61 ccggcgcaac aggcattcct cgatgccgcc gaactgatgc aatggagtat agaaaccgaa
      121 gggctgggct tgaacgtcat ctcgcacaag atactcggca aagaccacgc ccaagtcgaa
      181 tttgaagcct acttccaaga cggacaacac cgatccgcac atcacgaact gtccggcttc
      241 gtcaacatcg gcggacaatg gtatttcatc gatccgactg ttccgcatcc tgcgatgaaa
      301 caaccctgca tttgcggatc gggcaaaaaa ttcaaagcct gctgcggcaa atatctgcaa
      361 cctgtcgcat aaaaatgccg tctgaacgtt cagacggcat tttcaacgcg caaaaaaaac
      421 cattcatacc aagggtaagt atgaatggtc aatacattgc gggaaaacgt cttacttgct
      481 gcactgccga aaagggagaa acgacagtgt taaccagcaa aagcattgta cctttattaa
      541 tgttaaaaaa cattaatttt gaaaatatat taacgaatat atctggaacc tactgatttt
      601 cccttatcca cctgacccag cgttcaaaca aattaggttc gagtgcagca ataaccgaac
      661 gtttgaacac atgctgtccg ctccagaatt catcgccttc caatgtcgtc agcctgccgc
      721 ccgcctcctc gaaaatcaac gcacccgcag cataatccca cagcttctgc ccgccgtgaa
      781 cataaacatc ataacgcccg caagccagat aacaccagtc cagcgtgctg ctgcccatac
      841 tccgtatcgt tccaaaaggc gcgagcgtac tcatgcggct ggaaagtttg cccgaacgca
      901 gatatttgat ttccacgccc gcaatcgcct cattgagttt tttatccacg agacgcaaag
      961 gcaggcgcgt cccgtttaaa aacgccccct gcccccgttc ggcataaaaa cattcgccgc
     1021 tgaccggatt gtagattacg cccaactcgg cacgcccgtt gcggacaaac gccaccgaca
     1081 ccgcaaaatg cggcagcccg ttgacaaaat tatttgtccc gtctatcgga tcgacaatcc
     1141 acagcccctt ttcccccgaa tattgttccc acaaagccga ctgttcctgc cgcgacattt
     1201 cctcgcccaa catcgggcaa tcgatcagaa gcggcaacgc ggcggcaaaa gccgtctgcg
     1261 cggcaatgtc cgcctcgctc aacatcgaac cgtcttcctt gcggtgggat ggcgtattca
     1321 aaaaacgcgg cataatttcg gtttgcgcga tatggcgcac gactttctgc aaacggtgta
     1381 acacttccta ctgtcctcat attttgaact tgcggcgcgc gaacg
//
LOCUS       DAWFAD010000230         1402 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWFAD010000230 DAWFAD010000000
VERSION     DAWFAD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.230"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>1402
                     /locus_tag="WGO49_001997"
     CDS             <1..>1402
                     /locus_tag="WGO49_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0307047.1"
                     /translation="PGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQTAKNVDWKQVQ
                     LAYDKWDYKQEGLTGAGAAIVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAAT
                     TTVSTAAAMQTAALASLYSQAAVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGAL
                     NQMGADIAQLNSKVRTELFSSTGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKD
                     NLGNAALGALVNSFQGEAASKIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGA
                     AVGEIVAESILGGRNPATLSDAEKHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVV
                     NNALNFDSTPTNAKKHQPQKPDKTALEKIIQSIMPAHAAGAMTNPQDKDAAIWISNIR
                     NGITGPIVITSYGVYAAGWTAPLIGTAGKAAISTCMANPSGCTVMVTQAAEAGAGIAT
                     GAVTVGNAWEAPVGALS"
BASE COUNT          398 a          339 c          382 g          283 t
ORIGIN      
        1 ctcccggcgg ctatatcgtc gacattccga aaggcaatct gaaaaccgaa atcgaaaagc
       61 tggccaaaca gcccgaatac gcctacctga aacagcttca gacggccaag aacgtcgatt
      121 ggaaacaggt gcagctggcc tacgacaaat gggactataa acaggaaggc ttgaccggag
      181 ccggtgcagc gattgtggtg attattgtaa ccgctctaac ttatggatac ggagcggctg
      241 cagcgggtag cgtaactgcc gcaggaagta gtacagccgc agctgcaaca acgacagcgg
      301 cagcaactac cactgtttct actgcagctg ccatgcaaac cgcagcttta gcctccttgt
      361 atagccaagc agctgtagcc atcatcaata ataaaggcga tgtaggcaaa gcattaaaag
      421 atctcggcac cagtgatacg gtcaagcaga ttgttacttc cgcactgacg gcgggtgcat
      481 taaaccagat gggagcagat attgcccaat tgaacagcaa ggtaagaacc gaactgttca
      541 gcagtacggg caatcaaacc attgccaacc ttggaggcag attggccacc aacctcagca
      601 atgcaggtat ctcagctggt atcaataccg ccgttaacgg cggcagcttg aaagacaact
      661 taggcaatgc cgcattggga gcattggtta atagcttcca aggggaagcc gccagcaaaa
      721 tcaaaacaac cttcagcgac gattatgttg ccaaacagtt cgcccacgct ttggcgggtt
      781 gtgttagcgg actggtacag ggaaaatgta aagatggggc aattggcgca gcagttgggg
      841 aaatcgtagc tgaatccata cttggcggca gaaaccctgc tacactcagc gatgcagaaa
      901 agcataaagt tatcagttac tcgaagatta ttgcgggcag cgtggcggca ctcaacggcg
      961 gcgatgtgaa tactgcggcg aatgcggctg aggtagctgt ggtgaataat gctttgaatt
     1021 ttgacagcac ccctaccaat gcgaaaaagc atcaaccgca gaagcccgac aaaaccgcac
     1081 tggaaaaaat catccaaagt attatgcctg cacatgcggc aggtgcgatg actaatccgc
     1141 aggataagga tgctgccatt tggataagca atatccgtaa tggcatcaca ggcccgattg
     1201 tgattaccag ctatggagtt tatgcagcag gttggacagc tccgctgatc ggtacagctg
     1261 gtaaagcagc tatcagcacc tgtatggcta atccttctgg ttgtactgtc atggttacgc
     1321 aggctgctga agcgggcgcg ggaatcgcca cgggtgcggt aacggtaggc aacgcttggg
     1381 aagctccagt aggagcgttg tc
//
LOCUS       DAWFAD010000231         1393 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWFAD010000231 DAWFAD010000000
VERSION     DAWFAD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.231"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            28..1383
                     /locus_tag="WGO49_001998"
     CDS             28..1383
                     /locus_tag="WGO49_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ0307048.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLFYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKSY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLIITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMMGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
BASE COUNT          271 a          338 c          401 g          383 t
ORIGIN      
        1 ttttcaaatc aaaaaaggat ttaccttatg tcggaatata cgcctcaaac agcaaaacaa
       61 ggtttgcccg cgctggcaaa aagcacgatt tggatgctca gtttcggctt tctcggcgtt
      121 cagacggcct ttaccctgca aagctcgcaa atgagccgca tttttcaaac gctaggcgca
      181 gacccgcaca atttgggctg gtttttcatc ctgccgccgc tggcggggat gctggtgcag
      241 ccgattgtcg gccattactc cgaccgcact tggaagccgc gtttaggcgg ccgccgtctg
      301 ccgtatctgt tttatggcac gctgattgcg gttattgtga tgattttgat gccgaactcg
      361 ggcagcttcg gtttcggcta tgcgtcgctg gcggctttgt cgttcggcgc gctgatgatt
      421 gcgctgttgg acgtgtcgtc gaatatggcg atgcagccgt ttaagatgat ggtcggcgat
      481 atggtcaacg aggagcagaa aagctacgcc tacgggattc aaagtttctt agcgaatacg
      541 ggcgcggtcg tggcggcgat tctgccgttt gtgttcgcgt atatcggttt ggcgaacacc
      601 gccgagaaag gcgttgtgcc acaaaccgtg gtcgtagcat tctatgtggg cgcggcatta
      661 ctgattatta ccagtgcgtt cacaatcttc aaagtcaaag aatacgaccc ggaaacctat
      721 gcccgttacc acggcatcga tgtcgccgcg aatcaggaaa aagccaactg gttcgaactt
      781 ttaaaaacgg cacctaaagc gttttggacg gttactctgg tacagttttt ctgctggttc
      841 gccttccaat atatgtggac ttactcggca ggcgcgattg cggaaaacgt ctggcacacc
      901 actgatgcgt cttccgtagg ttatcaggag gcgggcaact ggtacggcgt tttggcggcg
      961 gtgcagtcgg ttgcggcggt gatttgttcg tttgtattgg cgaaagtgcc gaataaatac
     1021 cataaggcgg gttatttcgg ctgtttggct ttgggcgcgc tcggcttttt ctccgttttc
     1081 ttcatcggca accaatacgc gctggtgttg tcttatacct taatcggcat cgcttgggcg
     1141 ggcattatca cttatccgct gacgattgtg accaacgcct tgtcgggcaa gcatatgggc
     1201 acttacttgg gcctgtttaa cggctctatc tgtatgccgc aaatcgtcgc ttcgctgttg
     1261 agtttcgtgc ttttcccgat gatgggcggc ttgcaggcca ctatgttctt ggtagggggc
     1321 gtcgtcctgc tgctgggcgc gttttccgtg ttcctgatta aagaaacaca cggcggggtt
     1381 tgagcgatga gcg
//
LOCUS       DAWFAD010000232         1380 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWFAD010000232 DAWFAD010000000
VERSION     DAWFAD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.232"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(73..996)
                     /locus_tag="WGO49_001999"
     CDS             complement(73..996)
                     /locus_tag="WGO49_001999"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ0307049.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPAFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          370 a          328 c          341 g          341 t
ORIGIN      
        1 ttttggtttt ctgtttttgt aggaatgatg aaaatttaag ttttaggaat ttaccggaaa
       61 aaacagaaag cgttatccac aatttctgat gttcagctcg tgaaatgcgt cgggcaaatc
      121 atcgctgtcg gcaaattcca cccggtcgta agccgtttcg tctgccaaaa ccgcgcgcaa
      181 gagtgcgttg ttgatggcgt gtcccgattt gtagccttca aacgcgccga caatcggatg
      241 tccgacgata tacaaatcac cgatggcatc aaggattttg tggcgcacaa actcatcggg
      301 atagcgcaag ccttcaggat tcaggacatc cgtgtcgtca atcacgatgg cgttgttcaa
      361 attgccgccc aaacccagat tgtgggcgcg catcatttcc acttcgtgca taaagccgaa
      421 ggtgcgcgcg cgcgcgattt cgtcgatgta ggatttgccg gcgaaatcga tttcaaaagt
      481 gggcgagctg cggttgaaag ccggatggtc gaattcgatg gtcagcgtta ccttaaagcc
      541 gtcatacggc gtaaagcgca cccatttgcc cgcttctttg atttcgacag gcttgaggat
      601 tttcaaaaaa cgcttttgcg ccttttgatc gaccacgccc gcatcttgca aaaggtaaat
      661 aaacggcagg ctggagccgt ccataatcgg gatttcgggt gcgttcagct caatcagcgc
      721 gttgtcgata ccgtaggcgg acagcgcgga cataatgtgt tcgatcgtgc cgacgcgcac
      781 gcctttgtcg gtaacgatgg tggaggaaag gcgggtatcg ttgatcaaat aaggggtcag
      841 cttgatttgt tcgcccatct cgccgtccaa atcggtacgg cggaaggaaa tcccgctgtt
      901 ttcaggcgcg gggtgcaggg tcagtgcgac ccgttcgccg gaatgcaggc cgactccggt
      961 aacgctgatg gatttcgcca aagttctttg cagcataaac cgcttcctta tcaatggggt
     1021 aagttgggga atgatacgat aaaaccggaa aaacaagcta tgtttttcca tagtatttgc
     1081 caatgtatcc gttttcaata cgtaagctgc ataaaaatgt atagtggatt aacaaaaatc
     1141 aagacaaggc gacgaacccg cctccctcct gaaaaacgca aaaaatgccg tccgaaaacc
     1201 tttcggacgg cattttcgcg tgaaccgtca ttcccacaag gacataaaac caaaacagaa
     1261 aaccaaaaac agaaacctaa aattcgtcat tcccacgaaa gtgggaatct agttcgttga
     1321 gtttcagtta tttagaataa attttgaaac ttaaatcccg tcattcccac gaacctacat
//
LOCUS       DAWFAD010000233         1327 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWFAD010000233 DAWFAD010000000
VERSION     DAWFAD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.233"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1..1327
                     /locus_tag="WGO49_002000"
                     /pseudo
     CDS             1..1327
                     /locus_tag="WGO49_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
BASE COUNT          433 a          265 c          310 g          319 t
ORIGIN      
        1 gggataatag aaagtagtta atatctgctt tagataaagg atttgatgga tcttttaaag
       61 agaagcattt tactttttta caatctgtga tgatggatct aacaaagtta ggtgttgaat
      121 atacaataga tggttggcaa aaaattggag gttggggtaa tgggataatc aatgatttat
      181 ataaaagtgt tgtaaaaaga gagtggactg gaatatttga gatcgttaat aataacatca
      241 agcaaggaaa tgaagctttt aaaaatgaaa tcaatagctt ggttcatgat atgaaagctg
      301 ctggcaagga atttggagat gacttaaata cacagtggaa taatctcact caggctgccg
      361 aaataatcta taatgacata gtagacaata ctagtcaagg aatagaaaaa ggtgtcaaag
      421 ccattaaaga attgtctgaa aaaatgaaaa atgctgcttc cgatttggct gacggttcag
      481 cagagaaagc taaacaagta gtggaagatt tggctcaagc cgccaaagaa gcatacgaaa
      541 atgccaaatc cacagccgag aaggctgctc aagcagctcg agaatttttt aagggcttgc
      601 ccagttttaa agatctggcc gaaaaattta gagatctgtt cccaaatccg gaaggctgga
      661 tcgatgatgg tcaccaatgt ttcgctcctt gggttaaaga aactaaaaaa cgcaatggca
      721 aatatcatgt ctacgacccc cttgccctag atttggacgg cgacggcata gaaaccgttg
      781 ccaccaaagg cttttcaggc agcctatttg accacaaccg cgatggcatc cgcaccgcca
      841 ccggttgggt ttctgccgat gacggtctgc ttgtgcgcga tttgaacggc aacggcatca
      901 tcgacaacgg tgcggaactc ttcggcgaca acaccaaact ggcagacggc tcttttgcca
      961 aacacggcta cgcggctttg gccgaattgg attcaaacgg cgacaacatc atcaacgcgg
     1021 cagacgccgc attccaaacc ctgcgtgtat ggcaggatct caaccaggac ggcatttccc
     1081 aagctaatga attgcgtacc cttgaagaat tgggtattca atctttggat ctcgcctata
     1141 aagatgtaaa taaaaatctc ggtaacggta acactttggc tcagcaaggt agctatacca
     1201 aaacaaacgg tacaaccgca aaaatggggg atttactttt agcagccgac aacctgcaca
     1261 gccgttttaa agataaagtg gaactcactg ccgaacaggc aaaagccgcc aatcttgcgg
     1321 gcattgg
//
LOCUS       DAWFAD010000234         1326 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWFAD010000234 DAWFAD010000000
VERSION     DAWFAD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.234"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(172..609)
                     /gene="rnhA"
                     /locus_tag="WGO49_002001"
     CDS             complement(172..609)
                     /gene="rnhA"
                     /locus_tag="WGO49_002001"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ0307050.1"
                     /translation="MDTPVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDTLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            722..1111
                     /locus_tag="WGO49_002002"
                     /pseudo
     CDS             722..1111
                     /locus_tag="WGO49_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
BASE COUNT          250 a          330 c          398 g          348 t
ORIGIN      
        1 ctggattccc gcctgcgcgg gaatgacggt ggagcggttt cagttgtttc taaataaatt
       61 tttgcagcgt tgggttttta gattcccgcc tgcgcgggaa tgacggtgga ggacgatgcc
      121 gtctgaatcc cattagcggt ttcagacggc attttcttgt cggtgcgaca gtcaggaaaa
      181 ctgcgctgcg ccacggtttg ccaaatcgtc ggcgcgttcg ttttccgcgt gtcccgcgtg
      241 tcctttaacc caagtccaac tgacttggtg ctgcccgacc agagtgtcga gttctttcca
      301 taaatcgtcg tttttgacgg gctgtttggc ggcggttttc cagccgttgc gcttccaacc
      361 gtgtatccag ttttccatgc cgtttttgac gtattgcgag tcggtgcaga tgatgacggt
      421 gcagcggcgt ttgagcgatt tcagtccttc gatgacggcg gtcagctcca tgcggttgtt
      481 ggtggtttgc gcttcgccgc cgaaaagttc tttttcgtgg ctgccgtagc gcatcaacac
      541 gccccagccg cccgcgccgg gattgccttt gcacgcgccg tcggtgtaaa ggtaaacggg
      601 tgtgtccata ttgtgccttt gtgtggtgtc cggcgtgatt ataagcggtt tgcgcgccgt
      661 gccgtctgaa agattgcggc ggattgaaat aggcatataa tgtgcaactt tatttttgaa
      721 tatgaaagag cggatagtgg gtcaaagcgg cgagttgttt tgtttcgggc aaatgcccgt
      781 atggaaggcg gaaaacctgc cggaagtttt gttgtcgggc tattcgtccg aggaagggga
      841 atgggtctgc ctgaatgtgt tgcagggcga tgtcgaagtc cgcacgcagg acggggcggc
      901 ggaggtttgg tcggcagaaa gcggcgattg cgtgtttgcg ccgcagcagg tgttttcggt
      961 caaactgaaa acggacgatg ccgaaatccg tttgtcgctg tattgcgcgg cggcagacta
     1021 ttttcacaaa aaatacggga tgagtgccac gcattctgcg gtcgcggcgg cacgggatac
     1081 cgtaccggcg ggcagggcgt tggatatggg ttgagacctt tgcaaaattc ctttccctcc
     1141 cgacagccga aacccaaaca caggttttca gctgttttcg ccccaaatac cgcctaattt
     1201 tacccaaata cccccttaat cctgcccaga tacccgataa tcaggcatcc gtgctgcctt
     1261 ttaggcggca gcgggcgcac ttagcctgtt ggcggctttc aacaggttca aacacatcgc
     1321 cttcag
//
LOCUS       DAWFAD010000235         1272 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.235, whole genome shotgun sequence.
ACCESSION   DAWFAD010000235 DAWFAD010000000
VERSION     DAWFAD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.235"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..890
                     /locus_tag="WGO49_002003"
     CDS             <1..890
                     /locus_tag="WGO49_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0307051.1"
                     /translation="TAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVSEAKA
                     NLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNPALKNLSP
                     AQRRDFADNIIAEKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
BASE COUNT          350 a          373 c          325 g          224 t
ORIGIN      
        1 ccacggcgca aaaagaagcc gcacccgcac aacagcaggg cggtatccgc ttttcagacg
       61 gcattgccgc cgttgccgac aacgaagtca tcacgcgccg ccggcttgcc gaagccgttt
      121 ccgaagccaa agccaacctg cccaaagacg cgcagataag cgaatccgag ctgtcccgac
      181 aggtgctgat gcagcttgtc aaccaatccc tgattgtaca ggcgggcaaa cgccgcaaca
      241 ttcaagcaag cgaagcggaa atcgatgccg tcgtcgcaaa aaatcccgcc ctcaaaaacc
      301 tcagccccgc ccaacgccgc gattttgccg acaacatcat tgccgaaaaa gtccgccagc
      361 aggcagtgat gcagaacagc cgcgtgagcg aagctgaaat cgatgccttc ctcgagcagg
      421 cgcaaaaaca aggcatcacc ctgcccgaag gcgcaccgtt gcgccaatac cgcgcccaac
      481 acatcctgat taaagctgac agcgaaaacg ccgccgtcgg cgcggaaagc accatccgca
      541 aaatctacgg agaggcccgc agcggcacag acttttccag cctggcgcgc caatattcgc
      601 aagacgcgag cgcgggcaac ggcggagatt tgggctggtt tgccgacggc gtgatggttc
      661 ccgcctttga agaagccgtc cacgcgctca aacccggaca ggtcggcgcg cccgtccgca
      721 cccaattcgg ctggcatatc atcaaattga acgaagtgcg cgatgccggc acacctcagg
      781 aacgtatccg caattccgtg cggcaataca tcttccaaca aaaagccgaa caggcaaccg
      841 tcaacctgtt gcgtgacctg cattccggcg cgtatgtcga catccgctaa ggcggtttga
      901 agcaaaaagc cataccgatc ggcaaaaatc cgggcggtat ggctttttgg atttcgagtt
      961 acttttacac cgtcattcat cattcccgcg aaagcgggaa tctagaaacg aaaagtaaca
     1021 ggaatttatc gggaatggct ggaatttaaa ggtctggatt cccgctttcg cgggaatgac
     1081 gggattttgg gttgtggtaa tttatcggaa aaacaaaaaa acctatgccg tcattcccga
     1141 gcaggcggga atccggttat ttaaaactgc agaaatttat ccgaagcaac aacaatcttt
     1201 ccatcgtcat tcccgcgtag gcgggaatct agcaacgaaa agtaacagga atttatcggg
     1261 aaaacagaaa cc
//
LOCUS       DAWFAD010000236         1254 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.236, whole genome shotgun sequence.
ACCESSION   DAWFAD010000236 DAWFAD010000000
VERSION     DAWFAD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.236"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            237..1190
                     /locus_tag="WGO49_002004"
     CDS             237..1190
                     /locus_tag="WGO49_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HEZ0307052.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          200 a          273 c          432 g          349 t
ORIGIN      
        1 caaaccggat gattcgggct aaataattcc tgattctttg gatatagcca acggtatgcc
       61 tgccgatgcc gtcagaaagg gcttgatggg cgtttgcggt tcgggttaat atttgtgctg
      121 tatctgttaa ttccggttag taaaatgggg cggggacgat gagtggccgt cggtcggttt
      181 tgcacgggcg gtaataatgt ccggcgcaag gtgcgccgtt tcgggagggg tgtgtgatgg
      241 gttttctgat tggtttgagc cgcaacctga cgcggtttac ggctttggtc attttgcttg
      301 cgtcggcggt tgcgtttatc ggaccggctt cgtttgcgtg ggttaagggc gatgttcagg
      361 tcttggtgct gggcatcatt atgctgggta tggggatgac tttgggcagg gaggactacc
      421 ggattttggc gcagcgtcct ttggatgtgt ttatcggtgc ggcggcgcaa tataccgtga
      481 tgccgttgct cgccatcggt attgccaagc tgttcgacct ttcgccgggg ctgactttgg
      541 ggctggtgtt ggtggggacg tgtccgggcg gcgtgtcgtc caacattatg agtttccttg
      601 ccaaaggcga tgtggcgttt tcagtcggta tgacgacggt gtctacggtg ctggctccgg
      661 ccgttacgcc tttgtggatg acctgcctgg tcggtcagac ggtggatatg gacggttggg
      721 ggatgttccg gtttatgctg ctggtcacgc ttttgccggt ggtgctgggt tcggcggcaa
      781 atatcctgct gcacggaaaa cattggtttg aaaatgtccg tgccgttatg ccgggcgtgg
      841 cggtattggc atttgcctgc attgtcggcg gcgtggcttc ggttcacgga caccgttttg
      901 ccgaatcggc gttggtaatg gtgttggcga ttgcggtaca caatatcgcc ggctatgtgt
      961 caggctatta cgccgccgcg ctgtttggta tggatacggc aaaaaaacgc accttggcaa
     1021 tcgaagtcgg cgtgcagaat gccggtttgg caacaggatt gagcgcgaaa tttttcccag
     1081 gcaaccccga atcggcggtt gcggcggcgg tcgcctgcgt gtggcattcg gtttcgggga
     1141 cggtattggg caacttgttc gctctgtggg acaagcgtcg ggacgcttag tcgggcgtgt
     1201 ccgaaccgtc ccacgatata tagtggatta actttaaacc ggtacggcgt tgcc
//
LOCUS       DAWFAD010000237         1233 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.237, whole genome shotgun sequence.
ACCESSION   DAWFAD010000237 DAWFAD010000000
VERSION     DAWFAD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.237"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            41..373
                     /locus_tag="WGO49_002005"
     CDS             41..373
                     /locus_tag="WGO49_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ0307053.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCAMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            705..1214
                     /locus_tag="WGO49_002006"
     CDS             705..1214
                     /locus_tag="WGO49_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ0307054.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          328 a          233 c          295 g          377 t
ORIGIN      
        1 gccttgtttt gacggaaaac catcatataa aggaacactt atgcagattt tatcttttca
       61 gccggacatt gcggaacgta tgctggaagg tacggaaggc gagtcggtca acgaaaacgc
      121 acaattcgtc cgtacggaca acggctattg gattgcgtgg catgaaggcg tagcggcact
      181 gcttgcgccc gatacgccgc cgggcattcc ctgtttttgg gtggaaggcg cggaaagcct
      241 tgaagagttg tgcgccatgg tggaacgcgg cgagtttgac gaagtggaag agtttgacgg
      301 cgatgacgac gaatggctcg aaacggcaca gggttgcggg caccacggcg acgcttgcgc
      361 ctgcggacat taaaggcatt gcaggcttgc cgcaaggggg gcaaggcttt gccgtttttt
      421 aaataatcgg tggattgcat ctgtttgcaa tgggctgaac gcttcccttt gtattttatt
      481 gaataaaaaa acttatcttg gtattataat taaggcagca tcaattattt tgggatggca
      541 ataaacgcaa agcattgatt tgcgccgatt gcagacttat tatagcaggt tgcggcgcgg
      601 acttaatggg aattgtttat agagcatgat ggtctgaaac atcattaatt aaaaatacca
      661 ttaatccgat ttatatttat ttcaatttca atggaaaaac atcaatgaca atgattttaa
      721 gcattttaag cctgtttttt atcatcagac tgttattttt agccgtctct attaaacatg
      781 aaaaagcctt gattgccaaa ggggcaaaac aatacggaaa aaccaattcc acgctgcttg
      841 cggcagttca tacgctttat tatttggcgt gttttgtttg ggtatggctt tctgacactg
      901 cttttaatgg catatccttg attggtacgc tgacggtgat ggcttcgttt gtgatattgt
      961 cattgattat taagcagttg ggggagattt ggacggttaa aatctatatt ttaccaaatc
     1021 atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca ccccaattat tttttaaaca
     1081 tcatacccga actgattggc atcgccttat tatgtcaagc gtggtatgtt ttattgattg
     1141 gcctgcccat ttatttgctg gtcttattta agcgtatccg acaagaagaa caggcgatgg
     1201 caacactttt ttaacccgtt tcatcaatta tag
//
LOCUS       DAWFAD010000238         1202 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.238, whole genome shotgun sequence.
ACCESSION   DAWFAD010000238 DAWFAD010000000
VERSION     DAWFAD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.238"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            388..543
                     /locus_tag="WGO49_002007"
     CDS             388..543
                     /locus_tag="WGO49_002007"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ0307055.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(633..1082)
                     /locus_tag="WGO49_002008"
     CDS             complement(633..1082)
                     /locus_tag="WGO49_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0307056.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYIEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT          400 a          262 c          178 g          362 t
ORIGIN      
        1 ttatgtctaa aggtgcaaac atgccttaaa ttaccatagt ggattaaatt taaatcagga
       61 caaggcgagg caacgctgta ctggtttaaa tttaatccac tatatattgc ataggcaaaa
      121 tacaagccta taacgaattg gaaacaaaat gccgtctgaa aacatcttca gacggcatta
      181 taaaatctgt tcaccttttc agatgagtaa tgtacaccct tatacaattt ttgctactat
      241 gccccataaa tccacggcta aagatatcct tattatgtcc tatgatttat cgaaacgact
      301 tgtaatcggc ttagcatcaa gtgccctatt cgacttatcc gaatcggata atatatttag
      361 aatggaaggg gcagaaacct ataggcaata tcagagagaa aaacaaaacc atcccctaaa
      421 aaaggcgttg tctttccatt tattaaaaaa cttctgtcaa tcaatgaaat aaacccaaac
      481 gacccaacga ttgggtttat tcttttatcc agaaacaatc cagatacaga ttacgagtca
      541 taactatagg cttaatatta cacgattttc attccatcaa ggcggaaaac cgcacaaata
      601 ctgaaacact atcgatcgat ttgtaaacaa gcctacttaa gtaacttgca gtccttatca
      661 tttcctttaa aataatccag cccgtcacta cacgaactgg cggacttctt gcaaataaag
      721 gttactagat tttcattcat cttaataata aaaggatttt tatctttatc tatggctacc
      781 gccttcaaca tgaatttact gtctaaggcc ccgcgcgcga ttccattcaa acggatacaa
      841 aagccttctg cctctttaat cggcaaactt ggccatttgg tagatgtttg tttaaatctc
      901 ccattctgca gataaaactt ttccataaaa tgtgcatttt ctaacaaggc tgcccgcact
      961 gcatttatct ttgctttctc aatataattg cgatagctcg gataaacaat taaagcaagt
     1021 acagacaata tcaagaccac tgatattaat tcaaccagcg taaacccccg attatcagtc
     1081 attactttac ttccaataag aacagattat tcaacatatt tctttgaaca gacttactat
     1141 cccattcaac agtatgcata tttcccactc tattttttag cggccggtat agccggtttg
     1201 gc
//
LOCUS       DAWFAD010000239         1185 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.239, whole genome shotgun sequence.
ACCESSION   DAWFAD010000239 DAWFAD010000000
VERSION     DAWFAD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.239"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            5..946
                     /gene="mafA"
                     /locus_tag="WGO49_002009"
     CDS             5..946
                     /gene="mafA"
                     /locus_tag="WGO49_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0307057.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPETAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            950..>1185
                     /locus_tag="WGO49_002010"
     CDS             950..>1185
                     /locus_tag="WGO49_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ0307058.1"
                     /translation="MKPLRRLTNLLAACAVAAAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGQINVIQDYTHQMG"
BASE COUNT          291 a          455 c          261 g          178 t
ORIGIN      
        1 tgccatgaaa accctgcttc tcctcatccc cctcgtccta accgcctgcg gcacactgac
       61 cggcataccc gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac tcgtcgccgc
      121 atcgtcccgc gccgccgtca aagaaatgga tttgtccgcc ctgaaaggac gcaaagccgc
      181 cctttacgtc tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg gacgctactc
      241 tatcgacgca ctgatacgcg gcggctacca caacaacccc gaaagtgcca cccaatacag
      301 ctaccccgcc tacgacacta ccgccaccac caaatccgac gcgctctcca gcgtaaccac
      361 ttccacatcg cttttgaacg cccccgccgc cgccctgacg aaaaacagcg gacgcaaagg
      421 cgaacgctcc gccggactgt ccgtcaacgg cacgggcgac taccgcaacg aaaccctgct
      481 cgccaatccc cgcgacgttt ccttcctgac caacctcatc caaaccgtct tctacctgcg
      541 cggcatcgaa gtcgtaccgc ccgaatacgc cgacaccgac gtattcgtaa ccgtcgacgt
      601 attcggcacc gtccgcagcc gtaccgaact gcacctctac aacgccgaaa cccttaaagc
      661 ccaaaccaag ctcgaatatt tcgccgtcga ccgcgacagc cggaaactgc tgattgcccc
      721 tgaaaccgcc gcctacgaat cccaatacca agaacaatac gccctctgga tgggacctta
      781 cagcgtcggc aaaaccgtca aagcctcaga ccgcctgatg gtcgatttct ccgacatcac
      841 cccctacggc gacacaaccg cccaaaaccg tcccgacttc aaacaaaaca acggtaaaaa
      901 ccccgatgtc ggcaacgaag tcatccgccg ccgcaaagga ggataaaccg tgaaaccgct
      961 gcgcagactg acaaacctcc ttgccgcctg cgccgtagcg gccgccgcct tcggacagcc
     1021 cgccctcgcg gcggacttgg cgcaagatcc gttcattacc gataacgccc aacggcagca
     1081 ctacgaaccc ggcggcaaat accacctctt cggcgacccg cgcggcagcg tctccgaccg
     1141 caccggtcaa atcaacgtca tccaagacta tacccaccag atggg
//
LOCUS       DAWFAD010000240         1151 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.240, whole genome shotgun sequence.
ACCESSION   DAWFAD010000240 DAWFAD010000000
VERSION     DAWFAD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.240"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..429
                     /locus_tag="WGO49_002011"
     CDS             <1..429
                     /locus_tag="WGO49_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0307059.1"
                     /translation="AIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYTPMRQTGQ
                     PVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPDGQYMRTV
                     DVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            436..945
                     /locus_tag="WGO49_002012"
     CDS             436..945
                     /locus_tag="WGO49_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307060.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
BASE COUNT          400 a          199 c          197 g          355 t
ORIGIN      
        1 gctataccaa cccagacagt taaagaactt gatggcttac tacaagaatc aaaaaatata
       61 ggtgctgtaa atacacgaat taatatagcg aatagtacta ctcgatatac accaatgaga
      121 caaacgggac aaccggtatc tgctggcttt gagcatgttc ttgaggggca cttccatagg
      181 cctattgcga ataaccgttc agtttttacc atctccccaa atgaattgaa ggttatactt
      241 caaagtaata aagtagtttc ttctcccgta tcgatgactc ctgatggcca atatatgcgg
      301 actgtcgatg taggaaaagt tattggtact acttctatta aagaaggtgg acaacccaca
      361 actacaatta aagtatttac agataagtca ggaaatttga ttactacata cccagtaaaa
      421 ggaaactaac taaatatgag taactttgaa aaaaaatata ttttagaatt aaatgatgct
      481 ttaagccatt taaatcataa ctctacctca tttgatttat tgaaagtttt gatttcatgg
      541 ttatcaaacg atattgtcat tgataaattt aaaattttag gttatgactt tagtaaatat
      601 atcgaaatga atcccgatga ctatccggtt gaaaaatcta tattgaatag agaggaaatt
      661 atttatctca aaaacaatat ttatcgtaaa atatcatcag gaaattttaa atttcaatac
      721 tttgtacaat atattagaga tattttagaa tatttattta ttgaacatat tgaaagagtc
      781 tgtccttact gcgaatgggg tgaaatgcaa aaattagaag aacaaaatac gcatgaaacg
      841 gtgtatctct gtactcaatg tggatgtgct ttttataacg ataattcaca atttttatta
      901 aaaacccctt taaccattcc aatgaaacgt gatgaattta aataaacaag ccgtagcctg
      961 catgaaccct aaaatccacg tgtagcgtgt gtgcgccagc acgcatgcgt tccatgattt
     1021 acggctcaat gccgtctgaa aagctcacaa tttttcagac ggcatttgtt atgcaagtaa
     1081 atattcagat tccctgtatg ctgtacagac gcgggagtgt taagcccccc ttgtttgaag
     1141 ctccgcggct c
//
LOCUS       DAWFAD010000241         1144 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.241, whole genome shotgun sequence.
ACCESSION   DAWFAD010000241 DAWFAD010000000
VERSION     DAWFAD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.241"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            32..>1144
                     /gene="tuf"
                     /locus_tag="WGO49_002013"
     CDS             32..>1144
                     /gene="tuf"
                     /locus_tag="WGO49_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0307061.1"
                     /translation="MGHVDHGKTTLTAALTTILAKKFGGAAKAYDQIDNAPEEKARGI
                     TINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREH
                     ILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDDCPIVQGSALK
                     ALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRGTVVTGRVERG
                     IIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVL
                     AKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMV
                     MPGENVTITVELIAPIAMEEGLRFAIREGGRTVGAGV"
BASE COUNT          296 a          328 c          300 g          220 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgcccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gccaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatt gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg cttacgaaga
      541 aaaaatcttc gaactggctg ccgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaaccg ttcctgctgc ctatcgaaga cgtgttctcc atttccggcc gcggtacagt
      661 agtaaccggc cgtgtagagc gcggtatcat ccacgttggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa acccaaaaaa ccacctgtac cggcgttgaa atgttccgca aactgctgga
      781 cgaaggtcag gcgggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 agagcgtggt caggtattgg ccaaaccggg tactatcact cctcacacca agttcaaagc
      901 agaagtgtac gtactgagca aagaagaggg cggccgccat accccgttct tcgccaacta
      961 ccgtccccaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaatgccgg gtgagaacgt aaccattacc gtagaactga ttgcgcctat
     1081 cgctatggaa gaaggcctgc gctttgcgat tcgcgaaggc ggccgtaccg tgggtgccgg
     1141 cgtg
//
LOCUS       DAWFAD010000242         1070 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.242, whole genome shotgun sequence.
ACCESSION   DAWFAD010000242 DAWFAD010000000
VERSION     DAWFAD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.242"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(122..550)
                     /locus_tag="WGO49_002014"
     CDS             complement(122..550)
                     /locus_tag="WGO49_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ0307062.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(531..965)
                     /locus_tag="WGO49_002015"
     CDS             complement(531..965)
                     /locus_tag="WGO49_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ0307063.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTDKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
BASE COUNT          278 a          318 c          191 g          283 t
ORIGIN      
        1 tttgaaaacc ccattaaacc gtctttaacc catcgtcccg aacggtagat gtttcacccc
       61 tgcaaaccaa aaaaaagacc gtctgaatac agacggccaa agcctggtca cacatcactg
      121 cctaaaataa agctgcctgc tgtgccgcag gtcggctcat ttcattgata atcgtatatc
      181 ccgttcgtga agagatgccg tatttagggc atagcttcgt catcgccata agcccgctct
      241 ccttatcaat atcgcgcaat ttgacaaact cctgataaaa cctatggttt ctcaactgta
      301 ttaacgcctt gccgcaccgt gggacataca attcctcgcc accatatacc tgcaacagct
      361 catgtgtttt cacttcgccg atggcttcga ccaaaattgc caaacgctcg gtgtccacct
      421 tgcccttacc aaatttaaac cgcgccccgc caatcgcctt gaccagctgt tccgtcgctg
      481 ccagtccgat gacatccaca atgtccaaca cggtatccgg caataaatgt tcaacttttt
      541 cgaaccccat catccccacc cgctttttcc gttttcctgt tttccgcaat ctgcaacgca
      601 gcaaccagtt tatgtagctg cgtatcgtct aaatattcga ccttatcctt accaaacatc
      661 cgccgcgcca ttgcgtgtgc atagttccaa tgtttgccgc cgacggtcag cagggcttcg
      721 actttgtcca gcattgccgc tgatgatgtc cgacgcagat gcggtttgcc gtgtgggtta
      781 ccttttgctt taggcttaaa tccgtgcgac cgcatatcag cgacaacaga ctcaagttca
      841 gaaacatcca tatccgcaca cgaccgctta tccgtcacac gctccaacac cgcgcgatag
      901 gtaccgtcat ccaagcccag ctccttttga gcaatcttaa ttttcgcaat caacgcccgg
      961 cgcattatct ctctaataca acatatagta taaattagcg cattttatac aataaaatac
     1021 aatatatatt attaagccga tgtttttttg cgaaacggac agacataaaa
//
LOCUS       DAWFAD010000243         1032 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.243, whole genome shotgun sequence.
ACCESSION   DAWFAD010000243 DAWFAD010000000
VERSION     DAWFAD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.243"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(299..685)
                     /locus_tag="WGO49_002016"
     CDS             complement(299..685)
                     /locus_tag="WGO49_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ0307064.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGTSARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(682..927)
                     /locus_tag="WGO49_002017"
     CDS             complement(682..927)
                     /locus_tag="WGO49_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ0307065.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLTGIEKP
                     ARNAGRLKKFSHMGNTDISRILEDDNETAALFEESAL"
BASE COUNT          278 a          243 c          185 g          326 t
ORIGIN      
        1 cagccatata tattaagtac ctgctcttta tgcgcttttt taggaaagga agatcaaacg
       61 gttcaatagc gaagtcttgc ccaaatttga tggacaggat atagaataca tgcgtgcata
      121 ccacaccctg ccaaagttgg gagatgcact taaggttgca cttgtggtcg ccaactatgc
      181 ctatacacgc ggatttcccg cgtttatagg ccgcgacgga agtctggctt ttaagcgaag
      241 acaataaccg aaaagaccgc ctcggcggtc tttcttgtgt acacagtttt tgtatttgtc
      301 agcttgatgc attgacaact ctaattccat attgcggaat atattcatcg acagtcatca
      361 gttcaaagcc ttccgcttgg gtttgtgcaa tcaacatcct atcgaaaggg tctttgtgta
      421 tttccggaag gcttccagcc tgttttgcat gaaacagacc tataggcagc atttcaaaat
      481 cctcttcttg aagcacatca aaaaactctt ccggtaattt caacaacccc ttgttctgct
      541 tgatggaaat ttcccaaata cttgctgcac tgacaaagat cgcatttctc ggattttcta
      601 tcagtttgcg tgcagatgtc cccagtttct tgtcatccaa caaccaccac agcaaagcat
      661 gggtatcaag cagaatcttt ctcacagagc cgactcctca aataaagctg ccgtttcatt
      721 gtcatcctca agaatacgtg aaatatccgt atttcccata tgactgaatt ttttcaacct
      781 tcctgcattt cgtgccggtt tttcaatacc ggttagttgg acgcaaggct tacctgcttt
      841 cgcaataata acgatttccc ctgcttctgc tctttgaatc aattgactca aattggtttt
      901 tgcctgatga atatttgctt gaaacataac acttctcatg attagctaac ttaactaata
      961 tacatcatta ccaagatttt gggaatctca ttacatatat ttgattatat ccgccgtttt
     1021 attcacacct tg
//
LOCUS       DAWFAD010000244          987 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.244, whole genome shotgun sequence.
ACCESSION   DAWFAD010000244 DAWFAD010000000
VERSION     DAWFAD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.244"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(112..744)
                     /gene="mtrR"
                     /locus_tag="WGO49_002018"
     CDS             complement(112..744)
                     /gene="mtrR"
                     /locus_tag="WGO49_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ0307066.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          233 a          215 c          256 g          283 t
ORIGIN      
        1 ctatgttatt gcaaaggtct caatatgtat ttttagaaga aggctttgtg tcattttgat
       61 gccgtctgaa agggtttgtt cgtttcagac ggcattgtta ccaaggcttg attatttccg
      121 gcgcaggtcg ggatggtttt ccaagttgtc catcattatc ccgatgatgc gcggggcggt
      181 tttgcccaaa tcgaaacgtt cgcaggaaga gaaccaccgc caaatcagcc cgtccaaggt
      241 tgatttgatg aagatgaccg ctgtttcctt gtccaaatcg tcagccaaat cctgattttc
      301 caccgcttcg gtcaaaacgg cggtaatttt ctcgcgccag attgcctgat gcttgcgggc
      361 aatggcgata acggcggcgt tttgttccgt gtgttcgcat tttaaaaaca ggatgttgtg
      421 gaatttgtag tagatgtcgt tgctttgcag ccgctcgaaa aagtgcagca gcgtgtggcg
      481 gaataccgcc caagaccctc cttcggcatc ttcggcatct tgcgcgatgc agttttcgat
      541 gtcgtcgcag atacgttgga acagcgcgtc gaacaagtct tccttatttt tgaaatgcca
      601 atagagcgcg ccgcgcgtta cgccggcggc ttgggcgatt tcgttgagcg aggtgcgcgc
      661 aatccctttg cggtaaaagg tttccaaggc ggcaagcatc aggtgttctt tggttttcaa
      721 ggcttcggtt ttggtttttc tcataatggc gttttcgttt cgggtcggtt tgatgagggc
      781 ggattataaa aaagactttg taaccatgca atcgtgtatg tataatgaaa cccatgaaat
      841 tgagactaca tctcaacttt gaaaacaggc ataacctgct ttgcaacccg ttcgaacatt
      901 cgggtatcaa agcaggcggt ttttatatcc tgaaatatag tggattaaca aaaatcagga
      961 caaggcgacg aagccgcaga cagtaca
//
LOCUS       DAWFAD010000245          931 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.245, whole genome shotgun sequence.
ACCESSION   DAWFAD010000245 DAWFAD010000000
VERSION     DAWFAD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.245"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            57..782
                     /locus_tag="WGO49_002019"
     CDS             57..782
                     /locus_tag="WGO49_002019"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ0307067.1"
                     /translation="MPRFHLPENLSIGQTVNLPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAGAEILHEETADAESPLNITLIQSISSGERMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          244 a          314 c          215 g          158 t
ORIGIN      
        1 gtccgtttcc atcacgccgc tgcgacagat tataaccgtc cgaaccgcca aaaactatgc
       61 cccgattcca cctgcccgaa aacctttcca tcggacaaac cgtcaacctg cccgacaaca
      121 tcgtccgcca cctcaacgtc ctgcgcgtcc gccccaacga aaacatcacc ctcttcgacg
      181 gcaaaggcaa ggcacacgcc gcacggctga ccgttttgga aaaacgccgc gccggggcgg
      241 aaatcctgca cgaagaaacc gccgatgccg agtccccgct gaacatcacg ctcatccaat
      301 ccatatcttc cggcgaacgt atggacttta ccctgcaaaa aagcgtcgaa ctcggcgtaa
      361 ccgccataca gcccgtcatc agcgaacgct gcatcgtccg cctcgacggg gaacgcgccg
      421 ccaaacgcct cgcacgctgg caggaaatcg tcatctccgc gtgcgaacaa agcggcagga
      481 acaccgttcc ccccgtactg cccatcatcg gctaccgtga agcactcgac aaaatgccgt
      541 ctgaaagcac caagctgatt atgagcatca accgcgcccg caaactcggc gacatccgcc
      601 acccgtctgg cgcaatcgtc tttatggttg ggcccgaagg cggctggaca gaacaggaag
      661 aacaacaggc atttgaagct ggctttcagg cggttacact cggcaaacgg attttacgca
      721 cagaaaccgc cccactcgcc gccctcgccg ccatgcagac gctttggggc gatttcgcat
      781 aaacagaaat gccgtctgaa acccgttcag acggcatttt gcagccgatt aagatagtag
      841 gttcaaataa gatttcccgt gtcgtcattc ccgcgaaagc gggaatctag aaacgaaaaa
      901 ctacagggat ttatccgaaa caacaaccct c
//
LOCUS       DAWFAD010000246          926 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.246, whole genome shotgun sequence.
ACCESSION   DAWFAD010000246 DAWFAD010000000
VERSION     DAWFAD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.246"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..346)
                     /locus_tag="WGO49_002020"
     CDS             complement(<1..346)
                     /locus_tag="WGO49_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0307068.1"
                     /translation="MFLILGRNFLKIILCFSFFVPKFALASVNVPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLAR"
     gene            complement(693..>926)
                     /locus_tag="WGO49_002021"
     CDS             complement(693..>926)
                     /locus_tag="WGO49_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307069.1"
                     /translation="LPPTVTEEGKIIRPERVGDKWILNGKPVTLSYPECSNFEQIKQG
                     SYVGSTVLILFVVIFGFRLLINFLKDMGKVGTD"
BASE COUNT          343 a          203 c          125 g          255 t
ORIGIN      
        1 agcgggcaag tttgccgact cctgccaaga ctccggcgcg ggaaactgat gaagatacaa
       61 ccgaactaac tttacccgta cttgatgctc cagtagatac atgcctaact tttaaatctt
      121 gagcttctga agttaaatat cttcccgatt ctctatcaaa tacacctttc caaattcttc
      181 ttcttttgtc atctgaacct cgaataccta aatatctgcc atcatcataa acttcaaccc
      241 tatcaaattt acccggaaca tttactgatg ccaatgcaaa tttaggtaca aaaaaactaa
      301 agcataggat aatctttaaa aaatttcgcc ctaggataag aaacataaca ttttgcttca
      361 tctaatttaa ttaataatgt agaagaatca gggaattgaa tttctataaa atctctatca
      421 taacgatgaa agaaattcct aaaaatatca aatgaaaacg aagttttaaa aacccctttt
      481 ctaatcaatt ccctataata aattaacgct tgtggtaaat ctctataaat atgtgaatca
      541 gtttgaaatt taactaaatg atattcagaa ataaacataa ttcggacctt ttaaaacact
      601 ctaaaaagcc gattctacca accgttacca aaacagccaa gcaacagaca aagccaagaa
      661 gaaaccgaga agaaaccaaa aatcaataat catcaatcag taccaacctt gcccatgtct
      721 tttaagaaat taatcagaag cctgaaaccg aaaatgacta cgaacagaat tagaaccgtc
      781 gaaccgacat aagaaccttg ctttatctgc tcaaaattgg aacattccgg ataagacaac
      841 gtaaccggct ttccgttcaa aatccattta tcgcccaccc tttccggcct gatgattttt
      901 ccttcttctg taacagtagg aggaag
//
LOCUS       DAWFAD010000247          908 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.247, whole genome shotgun sequence.
ACCESSION   DAWFAD010000247 DAWFAD010000000
VERSION     DAWFAD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.247"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(152..>908)
                     /locus_tag="WGO49_002022"
     CDS             complement(152..>908)
                     /locus_tag="WGO49_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0307070.1"
                     /translation="FSSAAQAASEDSSRSPYYVQADLAYAAERITHNYPEPTGADKDK
                     ISTVSDYFRNIRAHSIHPRVSVGYDFGDWRIAADYASYRKWNNNKYSVNTKEVQRNNS
                     TGTENWQELKTENQENGSFHAASSLGLSAIYDFKLNDKFDKFKPYIGARVAYGHVKHQ
                     VHSVRKETTTVFSKPRGITEPGKVVKGPTSKPPYHESHSTSSLGLGVIAGVGFDITPK
                     LTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
BASE COUNT          182 a          199 c          260 g          267 t
ORIGIN      
        1 agattgcggt gttgtcggga atgacgggtt tcaagattac ggtgttgtcg ggaatgacgg
       61 tttgggtatt tccacgcccg ccccgcgcct gtaaacggca ggtgaatcaa aaatgccgtc
      121 tgaaggttca gacggcatcg gtgtcgggga atcagaagtg gtagcgcatg cccaatgaga
      181 cttcgtgggt tttgaatcgg gtgttttcca agcgtcccca gttgtggtag cggtatccgg
      241 tgtctaaagt cagcttgggc gtgatgtcga aaccgacacc ggcgatgaca ccaagaccca
      301 agctgctggt gctgtggctt tcgtgatagg gaggtttgga ggtcggacct tttacgacct
      361 tgcctggctc tgtaatgcct cttggtttac tgaaaacagt cgtggtttct tttctcactg
      421 aatgaacctg atgtttaacg tgtccgtagg cgacgcgcgc accgatatag ggtttgaatt
      481 tatcgaattt atcgttgagt ttgaaatcgt aaatggcgga taagccgaga gaagaagcgg
      541 cgtggaagct gccgttttcc tgattttccg tcttcagttc ttgccagttt tcagtgccag
      601 tgctattgtt tctttgcacc tcttttgtgt ttacggaata tttattgttg ttccattttc
      661 tgtaactggc ataatctgcc gctatcctcc agtcgccgaa atcgtagccg accgacacac
      721 gagggtggat ggaatgcgca cggatgtttc tgaaataatc gcttactgtg cttattttgt
      781 ctttgtctgc accggttggt tccggataat tgtgggtaat gcgttcggcg gcataggcta
      841 aatccgcctg cacataatac gggctgcggc tgctgtcttc acttgccgcc tgcgctgcgg
      901 aagagaag
//
LOCUS       DAWFAD010000248          905 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.248, whole genome shotgun sequence.
ACCESSION   DAWFAD010000248 DAWFAD010000000
VERSION     DAWFAD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.248"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..215
                     /locus_tag="WGO49_002023"
     CDS             <1..215
                     /locus_tag="WGO49_002023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307071.1"
                     /translation="DAGDVYCVYNIRLKQYTACQVTRVEEHGGKKTHATLLALDWQGN
                     KPLGAEELADLKPLYKDLKNNIGNIV"
     gene            546..>905
                     /locus_tag="WGO49_002024"
     CDS             546..>905
                     /locus_tag="WGO49_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307072.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQRYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLY"
BASE COUNT          282 a          147 c          211 g          265 t
ORIGIN      
        1 ctgatgccgg agacgtatat tgcgtctata acatcagact gaagcagtac actgcctgcc
       61 aggttacccg agttgaagaa cacggtggca aaaaaacaca tgcgaccctg ctggctttgg
      121 actggcaggg caacaaaccg cttggggcgg aggagctggc ggatttgaaa ccactttaca
      181 aagacttaaa gaataatatt ggaaatattg tatgaacaaa aaattaaact atatttttat
      241 gttggactgt ttagggttgg tgatattgtt tacttgtata atagctactt ttgaaagaga
      301 ttatggattt aaaattttta ctaattctaa gaggcctgaa ttttattatt ggattggaat
      361 gttttattat ggaattattt cttgctggtt tgattatcaa ttaatttcaa caaaggcgaa
      421 ttcgtataaa agaaaagtta aacaatataa aattttttca gtaatatttt cagttttgat
      481 atttatttct actatagtaa aactttaaat tttggagcaa aaatttatga gcgattcaat
      541 tgaatatgta ttgggaacgc ggtctgcaca tgtataaggc aagtgctgtc gtgccaacgg
      601 gatatgtacg ggttgggaat accgcgccgc tggtcggcga agacacgcaa cggtatgcct
      661 ctttttgggg cgacggctac gacgtgtacc gtcagttgag atggcagcag atacccgaaa
      721 aacagagaaa ggcattcaaa aaagccgcca aaagcaaaaa gaccgtgatg tttgccggac
      781 gggaatacgg catatccaaa cagaatttga gcgatgtttg ggatgatttt gaagacgcga
      841 tggaactgaa ggcgtttccc tgcctgtctt cgctgtttct gaccaagtgg cataaaaatc
      901 tatat
//
LOCUS       DAWFAD010000249          900 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.249, whole genome shotgun sequence.
ACCESSION   DAWFAD010000249 DAWFAD010000000
VERSION     DAWFAD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.249"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..247)
                     /locus_tag="WGO49_002025"
     CDS             complement(<1..247)
                     /locus_tag="WGO49_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0307073.1"
                     /translation="MCCSLSFAEPARIDDRIIKFRPSKSKFFESTGYRKINNEFSKFT
                     EAANVEHIPTGAKARINAKITASVSRAAVLSGVGKLVR"
     gene            complement(231..551)
                     /locus_tag="WGO49_002026"
     CDS             complement(231..551)
                     /locus_tag="WGO49_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0307074.1"
                     /translation="MNKPFITQAQLALYKYQPSSKYFGQSMALIASKEFEEFVRNVKE
                     YDVIECFSYFLNKRVTHNIWKIYFSDESNIFIRKSEENGKISHEFIYSEFSDSNTDFN
                     VLFS"
     gene            complement(670..>900)
                     /locus_tag="WGO49_002027"
     CDS             complement(670..>900)
                     /locus_tag="WGO49_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307075.1"
                     /translation="LPPTVTQDGKIIRPERVGDKWILNGKPVTLSYPKCSNYEQVKSG
                     AYLGSMVLILFVVIYGFRLLINFLKDIGKVGA"
BASE COUNT          279 a          189 c          157 g          275 t
ORIGIN      
        1 ggcggacaag tttgccgact cctgacaaga cggcggcgcg ggatacgctg gcggttatct
       61 ttgcgttgat tcgggctttt gcgcccgtgg ggatatgttc gacatttgcc gcttctgtga
      121 atttagaaaa ttcattattg atttttctat atcctgtaga ttcaaaaaac tttgatttag
      181 atggcctaaa ttttattatt cgatcatcta ttcttgctgg ttctgcaaaa ctaagagaac
      241 aacacattaa aatcggtgtt gctatcagaa aattccgagt aaataaattc atgcgaaatt
      301 tttccatttt cttctgactt cctaataaaa atattagact catcagaaaa ataaattttc
      361 caaatattat gcgttaccct cttatttaaa aaataagaga aacattctat tacgtcgtat
      421 tcttttacat ttcttacaaa ctcttcaaat tccttagacg caattaatgc catcgactgc
      481 ccaaaatact tgctggacgg ctgatattta taaagtgcca actgcgcctg cgtgataaac
      541 ggcttgttca tgtttctgtc tttcaaaggt tgttttgaaa gcctgatttt aaaacacgtc
      601 atataaatat caaagcgaca gacaaagcca ggaaaaatcc gagcaaaaac caaaaatcga
      661 caaacatcat cacgccccta ctttgcctat gtcttttaag aaattaatca gcagcctgaa
      721 gccgtaaata acgacaaaca gaattaaaac catagacccg agataagctc cggatttaac
      781 ttgttcgtaa ttcgaacatt tcggataaga cagcgtgacc ggctttccgt tcaaaatcca
      841 tttatcgccc accctttccg gcctgatgat ttttccgtcc tgggtaacag taggaggaag
//
LOCUS       DAWFAD010000250          880 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.250, whole genome shotgun sequence.
ACCESSION   DAWFAD010000250 DAWFAD010000000
VERSION     DAWFAD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.250"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(464..>880)
                     /locus_tag="WGO49_002028"
     CDS             complement(464..>880)
                     /locus_tag="WGO49_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0307076.1"
                     /translation="IIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKID
                     AIRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKEL
                     SDIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSF"
BASE COUNT          212 a          185 c          206 g          277 t
ORIGIN      
        1 taggtttcca tgcggacgcg ttcggattca cgactgcgcg gaaatgacgg gattttggtg
       61 tattccctaa aaaaataaaa aaacatttgc aactttgtta aaaataaagg ctgtgtttta
      121 acgatgtgtt gatatttaat tttagaaagg tagctattta atagttacct tttcttattt
      181 aaaaatagct ttctcaaatt ccatgaacgc ctcaatacga tatgcagatg ctctatcgaa
      241 attaagtttc aacattttgt ttattaaaca ttttatttta gccatttttc aatatacccc
      301 caaatatacc cccaatttgc acaagtcaaa attttaggca ggggttttgt tgctcccatg
      361 atacgcgggc ggatgccggc ttttcgcggg ggaaatacaa ggggtctcgg ttcgggtgtc
      421 aaaatccctg tttcgtgtta gtcatgtggg ggggaagagg gggttagaat gaagtaaagc
      481 tgttgccctc tccccgcaat agttccatta ggcgcggatg aatgaatagt ttgtccctgc
      541 cgatgacgat ttcttgcagc acacctatgt ctgaaagctc tttcaggtac ttagaggccg
      601 tctgccgttt ggctatccct gccgcttcta ggttggcaat gcgtgtatat ggctgctcaa
      661 acagaagatt taccagttcg tgcgtgtaga ttccttgtgc gtgtgtccgt atgtgttgcc
      721 gtgtctgctc gaacaggcgg cgtatcgcat ctattttcga taccgtccaa tcggcggtgt
      781 cggctacgcc gtctaagatg tagattatcc agctttccca atcctgccgt tcggttacgc
      841 ctaaaagcag gcggtaatag tccgccctgt tttcgatgat
//
LOCUS       DAWFAD010000251          867 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.251, whole genome shotgun sequence.
ACCESSION   DAWFAD010000251 DAWFAD010000000
VERSION     DAWFAD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.251"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(48..827)
                     /locus_tag="WGO49_002029"
     CDS             complement(48..827)
                     /locus_tag="WGO49_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ0307077.1"
                     /translation="MKLVRPQKISDQVLSVLEGRIVEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
BASE COUNT          200 a          288 c          173 g          206 t
ORIGIN      
        1 ttcactatac cgatacatcc gaatatgcca gaccgtctaa acggtattca tacctcccaa
       61 tcctgcacgc gcttcaaatc gttttgcgcc aaagtatccg cgtggcggtt acggctctga
      121 tattccctgt ctttcatgat gctgcttgcc acataattca aatgtgcctt tgccgcctcc
      181 gaagcctcgc ccggccgacg gttcgatatc gcctcataca gcacgcggtg ctgcgccatc
      241 agtttcggac gcggatcttc ttcctgattc agataaataa ggctgctgcg cgtctgccgg
      301 tacagcattt tcaacaaacc gcccgacaaa tggctgaaca acaaattgtg cgccgcatcc
      361 gcaatcgtct gatggaagct gacatcggct tcgctctgat gttccagatt gccgctttcg
      421 cacgcctcct caaacttttc aagccaaaac ccaatccgct tcaaatcggc atccgtccgg
      481 cgttctgccg ccaatgccgc catacagccc tcgatgtggc agctgaaatc aaaaacatcc
      541 tgttcccaat tggaatgctt gcccaaaagc tcctgccaac tttgcaaaaa atcctgctgc
      601 ggcttgaccg aaacataata accgtctccc tgcctcgctt ccaaaacctg acgggcgacc
      661 aaaacattca atgccgacct gaccgacggg cgggacacgc cgaactcttc cgccaaaacg
      721 cgttcgggcg gaatcttgcc cccttccgcg taaacccctt ccacgatgcg gccttccaat
      781 accgacaata cctgatcgct gattttctga ggccttacca gtttcatcac tcctccttta
      841 taaagattcc ctgccgaacc cttccga
//
LOCUS       DAWFAD010000252          807 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.252, whole genome shotgun sequence.
ACCESSION   DAWFAD010000252 DAWFAD010000000
VERSION     DAWFAD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.252"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            15..689
                     /locus_tag="WGO49_002030"
     CDS             15..689
                     /locus_tag="WGO49_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0307078.1"
                     /translation="MQADLAYAAERITHDYPKATGANNTSTVSDYFRNIRAHSIHPRV
                     SVGYDFGDWRIAADYASYRKWNNNKYSVNTKELENKLNNKKDLKTENQENGTFHAASS
                     LGLSAIYDFKLNDKFKPYIGARVAYGHVRHSIDSTKKTTEVVTSTHGGADTKPTIYNG
                     ESTQNAYHESHSIRRLGLGVVAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSL
                     GMRYHF"
BASE COUNT          234 a          228 c          177 g          168 t
ORIGIN      
        1 acggcccgta ttatgtgcag gcggatttag cttatgccgc cgaacgtatt acccacgatt
       61 atccgaaagc aaccggtgca aacaacacaa gcacagtaag cgattatttc agaaacatcc
      121 gtgcgcattc catccacccc cgggtgtcgg tcggctacga tttcggcgac tggagaatag
      181 cggcagatta tgccagttac agaaaatgga acaacaataa atattccgtc aacacaaaag
      241 agttggaaaa caagcttaac aataagaaag acctgaagac ggaaaatcag gaaaacggta
      301 cattccacgc cgcttcttct ctcggcttat ccgccattta cgatttcaaa ctcaacgata
      361 aattcaaacc ctatatcggt gcgcgcgtcg cctacggaca cgtcagacac agcatcgatt
      421 cgaccaaaaa aacaacagag gttgttacct ccacccatgg tggtgctgac acaaaaccta
      481 cgatttataa tggggaaagt acgcaaaacg cctatcacga aagccacagc atccgccgct
      541 tgggtcttgg tgtcgtcgcc ggtgtcggtt tcgacatcac gcccaagctg accctggaca
      601 ccggataccg ttaccacaac tggggacgct tggaaaacac ccgcttcaaa acccacgaag
      661 tctcattggg catgcgctac cacttctgat tccccgatac cgatgccgtc tgaaccttca
      721 gacggcattt ttgatttacc tgccgtttac aggcgcgggg cgggcgtgga aatacccaaa
      781 ccgtcattcc cgacaacacc gtaatct
//
LOCUS       DAWFAD010000253          802 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.253, whole genome shotgun sequence.
ACCESSION   DAWFAD010000253 DAWFAD010000000
VERSION     DAWFAD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.253"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..234
                     /locus_tag="WGO49_002031"
     CDS             <1..234
                     /locus_tag="WGO49_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0307079.1"
                     /translation="LIAQKEFEEFVNNVKEYDILESFSYFLNKRVAHNIWKIYFSDES
                     VIFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
     gene            176..616
                     /locus_tag="WGO49_002032"
     CDS             176..616
                     /locus_tag="WGO49_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307080.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLERSQIKFQSSNNLKSS
                     GFKLDSSSKSFAKFIEAANFEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKF
                     STRAVPYVGTALLAHDVYETFKEDIQARGYQYDPETDKFAKVSG"
BASE COUNT          249 a          148 c          182 g          223 t
ORIGIN      
        1 ttaattgctc agaaagaatt tgaagaattt gttaataacg taaaagagta cgatatttta
       61 gagagtttct cttatttctt aaataagaga gtcgcacata atatttggaa gatttatttt
      121 tctgatgaat ctgttatttt tataagaaaa tcagaagaga acggaaaaac tgttcatgaa
      181 ttcgtttatc aagagtacac cgatagttct gattttaatt ctatgttcga gtaattctta
      241 ttctgaacct gtaagattag aaagatcaca aattaaattt caatcaagta ataatttaaa
      301 atcaagtggt tttaaattag attcaagttc taagagtttt gccaaattca tagaagcggc
      361 aaattttgag cacatcccta cgggcgcaaa agcccgaatc aacgccaaaa taaccgccag
      421 cgtttcccgc gccggcgtat tggcgggggt cggcaaactt gcccgcttag gcgcgaaatt
      481 cagcacaagg gcggttccct atgtcggaac agccctttta gcccacgacg tatacgaaac
      541 tttcaaagaa gacatacagg cacgaggcta ccaatacgac cccgaaaccg acaaatttgc
      601 aaaggtctca ggctaagtgc gcctgttgcc gcctaaaagg taacccggat gcctgattat
      661 cgggtatccg gggaggatta agggggtatt tgggtagaat tagggagtga ttggtagcgg
      721 aaatagacga aaacctgtgt ttgggtttcg gctgtcggga gggaaaggaa ttttgcaaag
      781 gtctcttttc gtcattcccg cc
//
LOCUS       DAWFAD010000254          779 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.254, whole genome shotgun sequence.
ACCESSION   DAWFAD010000254 DAWFAD010000000
VERSION     DAWFAD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.254"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            371..655
                     /locus_tag="WGO49_002033"
     CDS             371..655
                     /locus_tag="WGO49_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0307081.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          233 a          147 c          196 g          203 t
ORIGIN      
        1 aaaacagcaa ccgctccgcc gtcattcccg cgcaggcggg aatccagaac gtaaaatcta
       61 aagaaaccgt gttgtaacgg cagaccgatg ccgtcattcc cgcgtaggcg ggaatctaga
      121 ccttgggata acggcaatat tcaaagttta taaaagaccc gttattcccg cgcaggcggg
      181 aatctagacc ttagaacaac agtaatattc aaaggttagc tgaagcttta gagattctag
      241 attcccactt tcgtgggaat gacgaagagt tgcgggaatg atggaaagct atgggaataa
      301 cgaaaggtta aagtaatcac gggatggtgt tcgcgggaat ataaatttaa ataattcaaa
      361 agggtattat atgcagcctg cggtttatat tttagcaagc caacgtaatg gcacgttata
      421 cattggcgtt acatctgatt tggtgcaacg tatttaccaa catagggaac atttgattga
      481 gggatttaca tcacggtaca acgttactat gctggtttgg tatgaactgc atcctacgat
      541 ggagagcgca attactcggg aaaaacagtt gaagaaatgg aacagggctt ggaaattgca
      601 actgattgaa gaaaataatg tttcttggca ggatttatgg tttgatatta tttagcccgt
      661 cattcccgcg caggcgggaa tctagaacca tcggcacgga aacttatcgg gtaaacggtt
      721 tcttgagatt ccgcgtccta gattcccact ttcgtgggaa tgacgggatt ttaggttcg
//
LOCUS       DAWFAD010000255          772 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.255, whole genome shotgun sequence.
ACCESSION   DAWFAD010000255 DAWFAD010000000
VERSION     DAWFAD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.255"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            88..606
                     /locus_tag="WGO49_002034"
     CDS             88..606
                     /locus_tag="WGO49_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307082.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGVIAVKKE
                     KGNYFLNKINVFTGKEESLLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARKALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLL"
BASE COUNT          227 a          157 c          221 g          167 t
ORIGIN      
        1 caactatagt ttttgcaaat caatattgac aagctgaaaa aaaaaaacaa tatactcgct
       61 cggtcttaat gttaacggag tatggaaatg aaacaaatgc ttttagccgt cggcgtggtg
      121 gcggtgttgg cgggctgcgg caaggatgcc ggcggttacg agggttattg gcgcgaaaag
      181 tcggacaaaa aagagggcgt gattgccgtc aaaaaagaaa aaggcaatta cttccttaat
      241 aaaatcaacg tatttacagg caaggaggag tctttgcttt tgtctgaaaa agacggcgcg
      301 ctttcgataa acacggggat aggggaaatc ccgatcaaac tttccgacga cgggaaagag
      361 ctgtatgtcg aacgcaggca gtatgtcaaa accgatgcgg cgatgaagga caaaatcatc
      421 gcccatcaga aaaagtgcgg acaaacggca caggcatacc tcgacgcgcg aaaggcgttg
      481 ccgtcaaacc aaacgtatca gcagcgtcag gcggcgatcg agcagttgaa acggcggttt
      541 gaagccgagt ttgacgaatt ggaaaaagaa atcaaatgca acggcaaacc gacattgttg
      601 ctttagcaga ggacaaccgg gaggatgccg ccgtccgaat cggacgtgcg gttttcgtac
      661 cggtacgggc gggcaggaat gtccgctttt tttgttcgga cgcgtttgaa tacccgtttg
      721 attccgtccg tttgcaaggg gtatttccgt tcgggcggaa attatagtgg at
//
LOCUS       DAWFAD010000256          760 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.256, whole genome shotgun sequence.
ACCESSION   DAWFAD010000256 DAWFAD010000000
VERSION     DAWFAD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.256"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(148..543)
                     /locus_tag="WGO49_002035"
     CDS             complement(148..543)
                     /locus_tag="WGO49_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307083.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDTETDKFVKVSG"
     gene            complement(527..>760)
                     /locus_tag="WGO49_002036"
     CDS             complement(527..>760)
                     /locus_tag="WGO49_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0307084.1"
                     /translation="VIAQSEFVEFAKINKSENVIDCFSFFWNRRIKHDIWLISFSDNS
                     EMVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
BASE COUNT          206 a          177 c          137 g          240 t
ORIGIN      
        1 tccctcccga cagccgaaac cccaacacag gttttcggct gttttcaccc caaatacctc
       61 ctaattttac ccaaataccc ccttaatcct ccccggatac ccgataatca ggcatccggg
      121 ttacctttta ggcggcaaca ggcgcactta gcctgagacc tttacaaatt tgtcggtttc
      181 ggtgtcgtat tggtagcctc gtgcctgtat gtcttctttg aaagtttcgt atacgtcatg
      241 ggctaaaagg gctgttccga catagggaac cgcccttgtg ctgaatttcg cgcctaagcg
      301 ggcaagtttg ccgccccccg ccaatacgcc ggcgcgggaa atgctggcgg ttattttggc
      361 gttgattcgg gcttttgcgc ccgtagggat gtgtgttaaa tctaccgttt ttattaaatc
      421 agatgaataa gttttactat ttttaggtac aaacttatga attttcgcac cttgtccggt
      481 atcaactgaa acagtttcag atatttttac tgcatttgca ttcgcttcaa acgaatacat
      541 catcaaaatt gcaattatcg acaatttcgc aaaattcaaa tttgtatatt ttatgaccat
      601 ctttcaggga ttctttaatt accatttctg aattatcaga aaatgagatt agccaaatat
      661 catgtttaat tcttctattc cagaaaaaag agaaacaatc aataacattt tcagacttat
      721 taatcttcgc aaattcaaca aattcagatt gcgctataac
//
LOCUS       DAWFAD010000257          752 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.257, whole genome shotgun sequence.
ACCESSION   DAWFAD010000257 DAWFAD010000000
VERSION     DAWFAD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.257"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            82..>752
                     /locus_tag="WGO49_002037"
     CDS             82..>752
                     /locus_tag="WGO49_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0307085.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCITARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLP"
BASE COUNT          230 a          202 c          161 g          159 t
ORIGIN      
        1 aatttttaaa catatatcct tgatatatac acggcgtaaa catatactgg aaacatcttt
       61 aaattttccg aaattttaaa tatgagcaac tggaaacccg atattcccta taacgattta
      121 ccacccctgc cgccaaaaca ggatattgaa agcaaaacca tcctgaaacg ttgtataacc
      181 gcccgtgcat cccttgcccg tttaaagcag gcggcagaac tgataccgaa tcaagccatg
      241 ctgattaaca cccttcctgt tatggaagcc cgtgcaagtt cggaaattga aaacatcgta
      301 accacaacgg ataagctgtt tcaatccctg caaatggata cggaacggca agaccctgcc
      361 acgaaagaag ccctgcaata ccgcaccgcc ctgtttgcag gctatgaatc actgacgagc
      421 cgccctttat gcacacaaac cgccatcatg gtctgcaacg ccatcaagca cccctacgaa
      481 atggccatcc gcaaaacagg cggcacagcc ctaaaaggag gcaacagcgg aaatgttgtc
      541 tataccccgc ccgaaggaga agaaaccata cgcggcaagc tggcaaattg ggagcggttt
      601 attcacgaaa gcggcgattt agacccgctt atcatcatgg cggcggcaca ttaccaattt
      661 gaagccatcc atccgtttac ggacggcaac gggcggacgg ggcgcatatt gaacagcctg
      721 ctattgattg aaaaagggct tttggatttg cc
//
LOCUS       DAWFAD010000258          724 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.258, whole genome shotgun sequence.
ACCESSION   DAWFAD010000258 DAWFAD010000000
VERSION     DAWFAD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.258"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(127..>724)
                     /locus_tag="WGO49_002038"
     CDS             complement(127..>724)
                     /locus_tag="WGO49_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0307086.1"
                     /translation="LSIGQIVKVKPAGYAAPKTSAVESRPAVPAAVQTPVKPAAQPPV
                     QSAPQPAAPAAENKAVPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGV
                     DIAGNAGQPVLAAADGKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQV
                     KRGQQVALMGNTDASRTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT          161 a          205 c          196 g          162 t
ORIGIN      
        1 aaacacagtc aaacaaattt atatgcccca tcccttccga ataatttgaa aacacagccg
       61 ccaaaaacaa aaatgccgtc tgaaaacctt tcagacggca tttccaattt gatttcaggc
      121 agaaagtcag aacgcgatat agctgttcgg gttaaccggt ttgccgtttt gccgcacctc
      181 gaaatgaagc tgcgttctgg aagcgtcggt attgcccatc aaagcgacct gctgcccgcg
      241 tttgacctgc tggccttcgc cgaccagcaa tttttggttg tgcccgtatg cggtcaggaa
      301 ggaagaatta tgctggatga tgaccagatt gccgtatccc ctcaaaccgg aacctgcata
      361 aaccactttg ccgtcagccg ccgccaaaac gggttgtccc gcatttcctg caatatcgac
      421 acccttgttg ttgccgccga aatcggcaac cactttacct tgcgtcggac gctgccaaac
      481 aatgccgccg accgaacgcg ttccggaagg cgaagcggca ggagattgcg gggcgggcgc
      541 gggaaccgct ttattttccg cagcgggcgc ggcaggttgc ggcgcggact gcacaggcgg
      601 ttgcgcggcg ggtttcacag gggtttgcac ggcagccggt acggcgggcc tgctttctac
      661 ggctgaggtt ttcggtgcgg catatcctgc cggtttgact ttaacaatct gaccgatgct
      721 caac
//
LOCUS       DAWFAD010000259          705 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.259, whole genome shotgun sequence.
ACCESSION   DAWFAD010000259 DAWFAD010000000
VERSION     DAWFAD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.259"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            28..705
                     /locus_tag="WGO49_002039"
     CDS             28..705
                     /locus_tag="WGO49_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HEZ0307087.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
BASE COUNT          218 a          137 c          156 g          194 t
ORIGIN      
        1 catcaaaaaa cctgataagc tcctaatatg aaactaaaaa ataagtatca aaagttcagc
       61 aaaatttcag agcaaaagtt tagagaaata atccgctgtt tcgctcttga tttgaccgct
      121 tccgatactg ctaaaatgac cggcattagt gtacgcggta tcaatcctat tttcctgaaa
      181 atcagacaca ggattgctgc tctgtgcgaa caaagctcac cactgtccgg tgtggtcgaa
      241 ttagatgaat cttactttgg tgcgcgacgt atcaggggta agcgcggtcg cggagcgtca
      301 ggtaaaacca tcgtatttgg catactgaaa cgcaatggcg tggtctatac ggaaatcgtt
      361 cccgacgctt cgaaagcctc actaatcaag gtcatacgtg gtcacatatc tgctgacagt
      421 gaaatcaata ctgacggctg gaaagcatat gacggtcttg tcgatatggg ctatgagaag
      481 cattaccgtg tccatcatgg ttccaatgag tttgctcgag gaaagcaaca tatcaatggt
      541 attgaatctt tttggagtta tactaagggg cgtttggcta aatttcatgg tatttccaaa
      601 gagatgtttt atctgcatct caaagaaacg gagtttaggt ttaaccaccg gcatgaagat
      661 ttaggtaaaa tattaatgaa gatgcttcga aataacccaa tttaa
//
LOCUS       DAWFAD010000260          693 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.260, whole genome shotgun sequence.
ACCESSION   DAWFAD010000260 DAWFAD010000000
VERSION     DAWFAD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.260"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>693
                     /locus_tag="WGO49_002040"
     CDS             <1..>693
                     /locus_tag="WGO49_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISNgo3 family transposase"
                     /protein_id="HEZ0307088.1"
                     /translation="KIKDYGKSRFNRTKTDKADSNLIADYIKRHQDTLIPYQIPKNKA
                     LQKLINLKNQLQQHQKQIKNRLHSTEEDFIRNIHQDLIDTIQDKMEQVKIAISEQIKK
                     QTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKTANQFVSYAGLSPAIIQSGTSVRG
                     RGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINNLKKAGKPKMVIIVAIMRKLAKLA
                     YYIVKTGQPYDAE"
BASE COUNT          273 a          157 c          124 g          139 t
ORIGIN      
        1 aaaatcaagg actacgggaa aagcaggttt aaccgtacca aaaccgacaa agcagattca
       61 aacctgatag cagattacat aaaaaggcat caagatacat tgataccgta tcagataccc
      121 aaaaacaaag cactgcaaaa actgattaac cttaaaaatc aattacagca acatcagaag
      181 caaattaaaa accgtcttca tagcactgaa gaagacttca taaggaacat acatcaagac
      241 ttgatagata ccatacagga caagatggaa caggtaaaaa tagccatatc cgaacaaatc
      301 aaaaaacaaa cggacaataa ccattaccgc aatcttcaaa ccatcccgag cataggcaaa
      361 gacaccgcat cagttcttta tgcgcaactg acagaaaaac attttaaaac cgcaaaccag
      421 tttgtatcct atgccggatt aagtcccgcc atcatacaat cagggacaag cgtaagaggt
      481 cggggcagat tgagccgata cggaaacaga cgattaaaaa gtacgctgta tatgcccgcc
      541 ctttgtgctt accgttttaa cgcatttccg aaattaataa ataatctgaa aaaagcgggt
      601 aagccaaaga tggtaatcat cgttgccatc atgcgcaaac tggcgaagct cgcctattac
      661 attgttaaaa ccggccagcc ttacgatgcg gaa
//
LOCUS       DAWFAD010000261          659 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.261, whole genome shotgun sequence.
ACCESSION   DAWFAD010000261 DAWFAD010000000
VERSION     DAWFAD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.261"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            62..659
                     /locus_tag="WGO49_002041"
                     /pseudo
     CDS             62..659
                     /locus_tag="WGO49_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          190 a          202 c          132 g          135 t
ORIGIN      
        1 gacctttgca aaattcccca aaatccccta aattcccacc aagacattta ggagatgttc
       61 catgagcacc ttctttcaac aaaccgcgca agccatgatt gccaaacaca tcgaccgttt
      121 cccgctattg aagttggatc agatgattga ttggcaactg atcgagcaat acctgaaccg
      181 tcaaaggacc cgttacctct gagaccaccg cggccgtccc gcctatcccc tgttgtccat
      241 gttcaaagcc gtcctgctcg gacaatggca cagtctctcc gatcccgaac tcgaacacag
      301 cctcattacc cgcatcgatt tcaacctgtt ttgccgtttt gacgaactga gcatccccga
      361 ttacagcacc ttatgccgtt accgcaactg gctggcgcaa gacgacaccc tgtccgaatt
      421 gctcaaactg attaactgcc aactgaccga aaaaggctta aaagtagaga aagcatccgc
      481 cgctgtcgtt gacgccacca ttattcagac cgccggcagc aaacagcgtc aggctataga
      541 agtcgataac aaaggacaag tcagcggcca aaccacaccg agtaaggaca gcgatgcccg
      601 ttggataaag aaaaacggcc tctacaaact cggttacaaa caacataccc gtaccgatg
//
LOCUS       DAWFAD010000262          652 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.262, whole genome shotgun sequence.
ACCESSION   DAWFAD010000262 DAWFAD010000000
VERSION     DAWFAD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.262"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WGO49_002042"
     CDS             complement(51..536)
                     /gene="fxsA"
                     /locus_tag="WGO49_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ0307089.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          195 a          211 c          116 g          130 t
ORIGIN      
        1 tcaaaccgga aaaatgccgt ctgaatcttc agacggcatt ccattcatat ttattcgtct
       61 tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt aaggctcttc aaccgtatat
      121 tccccctcga taatatcgtc atcgcgggaa aagccctctt ttctgcccga ttggttcatg
      181 ttgaaaaaat tttccgcacc tcctgcctgc aacactgcac ctcccttaaa cggcagcagc
      241 agcaataccg ccaacaccga ggatacgaat cccggactca tcagacacac cgccgccacc
      301 gtataacgga taggccacaa catctgataa acggataccc tcccgccgct tctcattgcc
      361 gcgcccgcca ataaaagacc ggacagcccc gtatgcctga gcatcagcac accggcggca
      421 aaacctgccg ccatcaaaaa caacgtccag ccgccgccca accaatcggc aacccacaca
      481 atcgacataa tctccaaaaa cagcagcacc aaaaaaccga taccgaaaaa tctcattgac
      541 cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac aagactccaa gcgacctgcc
      601 tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa tgtgaaatca cg
//
LOCUS       DAWFAD010000263          651 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.263, whole genome shotgun sequence.
ACCESSION   DAWFAD010000263 DAWFAD010000000
VERSION     DAWFAD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.263"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>651
                     /locus_tag="WGO49_002043"
     CDS             <1..>651
                     /locus_tag="WGO49_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated protein FrpC"
                     /protein_id="HEZ0307090.1"
                     /translation="GNDYLEGGSGSDTYVFGKGFGQDTVYNYDYATGRKDIIRFTDGI
                     TADMLTFTREGNHLLIKAKDDSGQVTVQSYFQNDGSGAYRIDEIHFDNGKVLDVATVK
                     ELVQQSTDGSDRLYAYQSGSTLNGGLGDDYLYGADGNDLLNGDAGNDSIYSGNGNDTL
                     DGGEGNDALYGYNGNDALNGGEGNDHLNGEDGNDTLIGGAGNDYLEGGSGSDTYVFG"
BASE COUNT          174 a          145 c          183 g          149 t
ORIGIN      
        1 ggcaatgatt acttggaggg cggcagcggt tcggatactt atgtcttcgg caaaggcttc
       61 ggtcaggata cggtctataa ttacgactac gctaccggac gcaaagacat catccgcttt
      121 accgacggta ttacagccga tatgctgact tttacccgag agggcaacca tcttcttatc
      181 aaggcaaaag acgacagtgg acaagtgact gttcagtcct atttccagaa cgatggctca
      241 ggtgcttacc gtatcgatga gattcatttc gataacggca aagtactgga tgttgccact
      301 gtcaaagaac tggtacagca atccaccgac ggttcggaca gattgtatgc ctaccaatcc
      361 ggaagtacct taaatggcgg attgggcgat gactatctgt acggtgccga cgggaatgac
      421 ctgctgaatg gtgatgcagg caacgacagt atctacagtg gcaatggcaa tgatacgctc
      481 gatggaggag aaggcaacga cgccctgtac ggctataatg gtaacgatgc actgaatggt
      541 ggcgaaggca atgatcattt gaacggcgaa gacggtaacg acactctaat cggcggtgca
      601 ggcaatgatt acttggaggg cggcagcggt tcggatactt atgtcttcgg c
//
LOCUS       DAWFAD010000264          626 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.264, whole genome shotgun sequence.
ACCESSION   DAWFAD010000264 DAWFAD010000000
VERSION     DAWFAD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.264"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          223 a          145 c          124 g          134 t
ORIGIN      
        1 attagttacc taaaacttaa aaaaaacaga aacctttccg cgtcattccc atgaaagtgg
       61 gaatctagaa cccaaatgct aaggcgattt atcggaaacg gctgaaaacg aagagattgg
      121 attcccgcct gcgcgggaat gacaactcat tagttaccta aaacttaaaa aacagaaacc
      181 tttacgccgt cattcccacg aaagtgggaa tctagaaccc aaatgctaag gcgatttatc
      241 ggaaacggct gaaaccgaac gaattggatt cccgccttcg caggaatgac aactcattag
      301 ttacctaaaa cttaaaaaac agaaaccttt acaccgtcat tcccacgaaa gtgggaatct
      361 agaacccaaa tgctaaggcg atttatcgga aacggctgaa accgaatgaa ttggattccc
      421 gcctgtgcgg gaatgacgac ttattagtta cctaacactt aaaaaacaga aacctttccg
      481 cgtcattccc acgaaagtgg gaatctagaa cccaaatgct aaggcgattt atcggaaacg
      541 gctgaaaacg aagagactgg attcccgcct gcgcgggaat gacgacttat tagttaccta
      601 aaacttaaaa aacagaaacc cttacg
//
LOCUS       DAWFAD010000265          618 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.265, whole genome shotgun sequence.
ACCESSION   DAWFAD010000265 DAWFAD010000000
VERSION     DAWFAD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.265"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1..548
                     /locus_tag="WGO49_002044"
                     /pseudo
     CDS             1..548
                     /locus_tag="WGO49_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245130.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          157 a          174 c          156 g          131 t
ORIGIN      
        1 cggcggatat gcggactgcc gagaaaaacc gtctgcatta ggttcatgaa acgcaagtcg
       61 gaagcgtcaa acaactgatt gcccattttg accggctgat tgacgaattg gacaaacaaa
      121 tcgacgacca cacccacacg cattttgacg gcaaagccca agtggcggag caaatcaaag
      181 gcatcggttc gataacgacg gctacgctga tggcgatgct gcccgaattg gggcggctgt
      241 cgcacaaacg gatagcgagt ttggccggca ttgccccgca cccgagggag agcggggaaa
      301 ccaaattcaa aagccgctgc tttggcggaa agtctgcggt acgtaaggcg ctgtatatgg
      361 ctaccgtggc agcgacacgt tttgaaccgc ttattcggga tttctaccaa cgcctgctgt
      421 ccgagggtaa gccgtataag gttgccgtta cggcatgtat gcgcaaactg ctggagacct
      481 ttgcaaaatt cctttccctc ccgacagccg aaacccaaac acaggttttc gtctattttc
      541 gctcctaatt actcctaatt ctacccaaat acccccttaa tcctccccgg acacccgata
      601 atcaggcatc cgtgctgc
//
LOCUS       DAWFAD010000266          608 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.266, whole genome shotgun sequence.
ACCESSION   DAWFAD010000266 DAWFAD010000000
VERSION     DAWFAD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.266"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..>608)
                     /gene="mafB"
                     /locus_tag="WGO49_002045"
     CDS             complement(<1..>608)
                     /gene="mafB"
                     /locus_tag="WGO49_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0307091.1"
                     /translation="HGHEVHSPFDNHDSRSTSDFSGGVDGGFTVYQLHRTGSEIHPED
                     GYDGPQGSDYPPPGGARDIYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFS
                     RADEAGKLIWESDPNKNWWANRMDDVRGIVQGAVNPFLMGFQGLGVGAITDSAVNPVT
                     YAAARKTLQGIHNLGNLSPEAQLAAATALQDSAFAVKDGINSA"
BASE COUNT          145 a          158 c          131 g          174 t
ORIGIN      
        1 gcagagttga taccgtcttt taccgcaaaa gcactgtctt gtaatgcggt tgcagccgca
       61 agttgtgctt ccggacttaa atttcctaaa ttgtgaatac cctgtaaagt tttccgtgct
      121 gccgcatagg ttaccgggtt taccgcactg tctgtaattg ccccaactcc caatccctga
      181 aaacccatta aaaaaggatt aaccgcacct tggacgatgc cgcgaacatc atccatacgg
      241 ttagcccacc aatttttatt ggggtcgctt tcccatatca gttttcctgc ttcatccgca
      301 cggctgaaaa aaccagaggc ggcaccggca ttttctttta gccaacggtc tgaaaatggg
      361 gcttgaggga caatattagt ctttgttttt gttgaagttc ctttgacata atagctgtat
      421 atatcccttg ctcctccggg gggcggataa tcgctgcctt gcggtccgtc atatccatcc
      481 tccggatgga tttccgaccc tgtccgatga agttggtaaa cagtaaaacc accgtctaca
      541 ccgccgctga aatcagaagt gcttcttgaa tcatgattat cgaacggact gtgcacttca
      601 tgtccatg
//
LOCUS       DAWFAD010000267          599 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.267, whole genome shotgun sequence.
ACCESSION   DAWFAD010000267 DAWFAD010000000
VERSION     DAWFAD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.267"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..150
                     /locus_tag="WGO49_002046"
     CDS             <1..150
                     /locus_tag="WGO49_002046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307092.1"
                     /translation="SIRQGKPEEEFSRKMAGCLTDLENCLEKYLEQFGPVSESIEACT
                     VKLQE"
     gene            172..465
                     /locus_tag="WGO49_002047"
     CDS             172..465
                     /locus_tag="WGO49_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307093.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPDCFDMKAVWQMFNRIPR"
BASE COUNT          158 a          150 c          160 g          131 t
ORIGIN      
        1 tccattcggc agggaaagcc tgaagaagaa ttttcccgta agatggccgg ctgcctgacc
       61 gacttggaaa actgtttgga aaaatacctc gaacagttcg ggccggtatc ggaaagtatc
      121 gaagcctgta ccgtcaaact gcaggagtaa ccgtcgttct tcaacaggct gatgaaaacc
      181 aacgacaagc tcaataggca gattgacgtg ttgcagaaac agtcggcagc catccacaac
      241 gaagcctata tcgaaatgaa cacgctgctt taccgccacc gcgaagtggt ttccgtccac
      301 aaccgcaagg cggattatgc ggaaaagggc aaagagcgga ttgcgctgtt cccgcgcggc
      361 ttgaacggca tcaccaagct gcctgccgcc gtcctgttgc ccgagcgtcc cgattgtttt
      421 gatatgaagg cagtttggca aatgtttaat agaataccga ggtaggcgta tcggctgccg
      481 gataaaaatg ccgtctgaaa cggaagcgtt cagacggcat tgttgcagtg gaacaaatca
      541 aaatgcaccc cgtcattcct gtctgtacgg tgatagcggc agtgttttcc tgcttcgtt
//
LOCUS       DAWFAD010000268          598 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.268, whole genome shotgun sequence.
ACCESSION   DAWFAD010000268 DAWFAD010000000
VERSION     DAWFAD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.268"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            308..>598
                     /locus_tag="WGO49_002048"
     CDS             308..>598
                     /locus_tag="WGO49_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0307094.1"
                     /translation="MQVFLTIPGYDVEAEIEKFVWMDAVIWQMPSWWMHEPWTVKKYI
                     DEVLTAGHGKLYQSDGRHSVNPTEGYGTGGLLQGKKHMLSLTWNAPIEAFTRE"
BASE COUNT          174 a          149 c          147 g          128 t
ORIGIN      
        1 actcattagt tacctaaaac ttaaaaagca gaaaccttta cgccgtcatt cccacgaaag
       61 tgggaatccg ggaacttaac gttacagtga tttatcggaa acggctgaaa ccgaatgaat
      121 tggattcccg cctgcgcggg aatgacgaat ccatccgtac ggaaacctgc accacgtcat
      181 tcccactaac ctacattccg tcattcccac gaaagtggga atccaggacg aaaaatctcc
      241 agaaaccgtt ttacccgata agtttccgca ccgacaggtc tggattcccg cctgcgcggg
      301 aatgacgatg caggtatttc tgacgattcc cggctatgat gttgaggcag aaatcgaaaa
      361 attcgtttgg atggatgctg tgatttggca gatgccgagc tggtggatgc acgagccttg
      421 gacagtgaaa aaatacatag acgaagtatt aaccgctgga cacggcaaac tctaccaaag
      481 cgacggcaga cacagcgtca atccgactga gggctacggc acaggcggct tgttgcaagg
      541 caaaaaacat atgctttcac tgacttggaa tgcgccgatt gaggcgttta cccgcgaa
//
LOCUS       DAWFAD010000269          578 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.269, whole genome shotgun sequence.
ACCESSION   DAWFAD010000269 DAWFAD010000000
VERSION     DAWFAD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.269"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          122 a          159 c          151 g          146 t
ORIGIN      
        1 tatgggaaaa catcaagatg gcggcataaa gcgcggtatg gcggaaaacc cgccttttta
       61 aagttttatt catcgtattt ccttttcggt caaacccccc gcccttcggg gcggcaggat
      121 caggctttgt ccgggagggg tgcaagccct tccgaatcag ggcggcgcct tatgtgccgc
      181 cctgcgtgtt ggaacatatg tttcgcaggg tgtttcgtgg gtttcgcagg gcgggctgta
      241 gggcgggctt tagcccactc tatggcgcac cttgcgaatc tgccttataa aaatacccga
      301 accgtcattc ccgcgcaggc gggaatctag gatgcggaat ctagagaaac cgtttatccg
      361 ataagtttcc gttccgacag gtctggattc ccgcctgcgc gggaatgacg gacgggaacg
      421 gcgcacgggc attgccggaa tcttttgtgt ttccattatc atactctttt taatgcgcta
      481 ttccaagaat atgatgccct ccggcggcat cccaccctac ggcttgctat aaaaatacct
      541 aaaccgtcat tcccgcaaac ctacattccg tcattccc
//
LOCUS       DAWFAD010000270          573 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.270, whole genome shotgun sequence.
ACCESSION   DAWFAD010000270 DAWFAD010000000
VERSION     DAWFAD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.270"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1..146)
                     /locus_tag="WGO49_002049"
                     /pseudo
     CDS             complement(1..146)
                     /locus_tag="WGO49_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
     gene            complement(169..354)
                     /locus_tag="WGO49_002050"
     CDS             complement(169..354)
                     /locus_tag="WGO49_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307095.1"
                     /translation="MGVCLDACCSAAGIARQIGKGGEIHGTLAFSLLFLSLGHIGMVA
                     YHHIKGESVLPRMLGKS"
     gene            354..518
                     /locus_tag="WGO49_002051"
     CDS             354..518
                     /locus_tag="WGO49_002051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307096.1"
                     /translation="MLNLVTAVLAGRFLRWRIPHPCISELLYHSNKPPDRHPRPPKAD
                     NRCVAQGAAG"
BASE COUNT          142 a          155 c          145 g          131 t
ORIGIN      
        1 tccactatat tttgatgcag acgtgtaccg atgtagcccg gctgggtttt caggaaatcg
       61 cgtgatttct gccaagcagc caaagtttcg gcttctttgc cctgcggcac ttcaaaggtg
      121 ttgatgagta cagcactgtt ctgcatgatt taaatttaat ccactatatt aagattttcc
      181 gagcattcga ggcagcacgg attcgccttt gatgtggtgg taggcaacca ttccaatgtg
      241 tcccaacgac aaaaacagca atgaaaatgc cagcgtgccg tgaatttctc cgcccttgcc
      301 gatttgacgg gcaatgcctg cggcggaaca gcaagcatca aggcatacgc ccaatgctca
      361 atttggtgac ggcggtgttg gcggggcggt ttttacgttg gcggattccc cacccttgta
      421 tatcggaact cctgtatcat agcaacaaac cgcccgaccg ccacccgcgc ccaccaaaag
      481 cagacaaccg ttgcgtagct cagggagcgg cagggtaacc catcgacaca accggacagt
      541 tgccggacaa cacaaccgaa tgcaaggcag gtt
//
LOCUS       DAWFAD010000271          555 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.271, whole genome shotgun sequence.
ACCESSION   DAWFAD010000271 DAWFAD010000000
VERSION     DAWFAD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.271"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..376
                     /locus_tag="WGO49_002052"
     CDS             <1..376
                     /locus_tag="WGO49_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0307097.1"
                     /translation="IKGIGSITTATLMAMLPELRRLSHKRIAGLAGIAPHPREGEETK
                     FKSRCFGGRSAVRKALYMATVAATRFEPLIRDFYQRLPSEGKPYKVAVTACMRKLLTI
                     SNARMRDYFAENDTAENGIRTA"
BASE COUNT          128 a          134 c          162 g          131 t
ORIGIN      
        1 aatcaaaggc atcggttcga taacgacggc tacgctgatg gcgatgctgc ccgaattgag
       61 gcggctgtcg cacaaacgga tagcgggttt ggccggcatt gccccgcacc cgagggaggg
      121 cgaggaaacc aaatttaaaa gccgctgctt tggcggaagg tctgcggtgc gtaaggcact
      181 gtatatggct accgtggcag cgacacgttt tgaaccgctt attcgggatt tctaccaacg
      241 cctgccgtcc gagggtaagc cgtataaggt tgccgttacg gcatgtatgc gcaaactgct
      301 gacgatatcg aatgcccgga tgcgtgatta ttttgccgaa aacgataccg ccgaaaacgg
      361 tatccggacg gcttgatttg agttttggta tttttgcccg acggggtgaa aaatacagtt
      421 gctacggctc gatgaatcgt cagaaatacc cgaaccgtca ttcccgcgca ggcgggaatc
      481 cagaccggtc ggtgcggaaa cttatcaggt aaaacggttt cttgagattt ttcgtcttgg
      541 attcccactt tcgtg
//
LOCUS       DAWFAD010000272          555 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.272, whole genome shotgun sequence.
ACCESSION   DAWFAD010000272 DAWFAD010000000
VERSION     DAWFAD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.272"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..351)
                     /locus_tag="WGO49_002053"
     CDS             complement(<1..351)
                     /locus_tag="WGO49_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307098.1"
                     /translation="MSANLVQPLNFSDGISLHDCYCTEIKVEGRNVCFDFEDGFVVPD
                     NNPNNQAGKHLKTDFSRVVLTHKNENGEDDYLVDIFEDIVFLGKRLFTVRKFLDFSEL
                     LEMVNTQGYFLEFLY"
     gene            366..>555
                     /locus_tag="WGO49_002054"
     CDS             366..>555
                     /locus_tag="WGO49_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0307099.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEVF"
BASE COUNT          179 a          123 c           97 g          156 t
ORIGIN      
        1 atacagaaac tcaagaaaat aaccttgtgt attaaccatc tcaagcaatt cagaaaaatc
       61 aagaaatttt ctgaccgtaa acaaacgttt ccctaaaaaa acgatgtctt caaaaatatc
      121 gaccaaataa tcatcttcgc cgttttcatt tttatgggtt aaaacaacac gggaaaaatc
      181 tgttttcaga tgcttgcccg cttgattgtt cggattattg tccggaacga caaaaccgtc
      241 ctcaaaatca aagcagacgt tgcgtccttc tacctttatc tctgtgcaat aacaatcatg
      301 cagagaaata ccatccgaaa aatttagggg ctgtactaga ttagcagata tgttaccctc
      361 gaaatatgaa gataacgcac tgcaaattaa agaaaaaagt acagaaagaa ctgctccgtt
      421 tttttgtgct ggaagttacc gcccgttctg ccgccgatat tttgggtatc catcccaatt
      481 cggcagcact gttctaccgt aaaatccgca cggttatcaa ccatcatttg gccttggctg
      541 ccgatgaggt ttttg
//
LOCUS       DAWFAD010000273          536 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.273, whole genome shotgun sequence.
ACCESSION   DAWFAD010000273 DAWFAD010000000
VERSION     DAWFAD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.273"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          163 a          126 c          107 g          140 t
ORIGIN      
        1 tcgcgggaat gacggcggag agggtttgtt gtttcggata aatccctgta gtttttcgtt
       61 tctagattcc cgcttttgcg ggaatgacgg cggggggatt tctgtttttc cgacaaattc
      121 ctaaaactta aaattccgtt attcccgcga aaacagaaaa ccaaaaatag aaacctaaaa
      181 tctcgtcatt cccgcaaaaa cagaaaacca aaaaacagca acctaaaatt cgtcattccc
      241 gcgcaggcgg gaatccagtc cgttcggttt cgcttgtttt aagtttcggg caacttccac
      301 ttcgtcattc ccgtgaaagt gggaatccag tccgttgagt ttcagtcatt tccgataaat
      361 caccgcaacg ttaaatttct agattcccgc ttttgcggga atgacggcgg gggggatttc
      421 tgtttttccg acaaattcct aaaacttaaa attccgttat tcccgcgaga acagaaaacc
      481 aaaaacagaa acctaaaatc tcgtcattcc cgcaaaaaca gaaaaccaaa aaacag
//
LOCUS       DAWFAD010000274          495 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.274, whole genome shotgun sequence.
ACCESSION   DAWFAD010000274 DAWFAD010000000
VERSION     DAWFAD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.274"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1..471
                     /locus_tag="WGO49_002055"
                     /pseudo
     CDS             1..471
                     /locus_tag="WGO49_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          132 c          121 g           84 t
ORIGIN      
        1 aactggctgg cgcaagacga caccctgtcc gaactgttgg aactgatcaa ccgccaactg
       61 accgaaaaag gtttaaaaat agagaaagca tccgctgccg ttgttgacgc caccattatt
      121 cagaccgccg gcagcacaca gcgtcaggct atagaagtcg atgaagaagg acaaatcaac
      181 ggccaaacca caccgagtaa ggacaaagat gcccgttgga tcaagaaaaa cggcctctac
      241 aaactcggtt acaaacaaca tacccgtacc gatgcggaag gctatatcga gaaactgcac
      301 atcacccccg ccaatgccca tgagtgcaaa cacctgtcgc cgttgttaga agggatagct
      361 gaaagtacga ccgtctatgc cgacaaaggc tatgacagtg cggaaaaccg gcaacatctg
      421 aaagaacatc agttgttgga cggcattatg cgcgggcagc ctatttcgga ctgctcaaag
      481 tgagtgcgca aagcc
//
LOCUS       DAWFAD010000275          493 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.275, whole genome shotgun sequence.
ACCESSION   DAWFAD010000275 DAWFAD010000000
VERSION     DAWFAD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.275"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>493
                     /locus_tag="WGO49_002056"
     CDS             <1..>493
                     /locus_tag="WGO49_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307100.1"
                     /translation="DNRKQLISALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDG
                     WQKIGGWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAP
                     WVKETKKRNGKYHVYDPLALDLDGDGIETVATKGFAGSLFDHTNNGIRTATGWVAADD
                     GLLV"
BASE COUNT          157 a           83 c          116 g          137 t
ORIGIN      
        1 cggataatag aaagcagtta atatctgctt tagataaagg atttgatgga tcttttaaag
       61 agaagcattt tactttttta caatctgtga taatggatgt aacaaagtta ggtgttgaat
      121 atacaataga tggttggcaa aaaattggag gttggggtaa tgggataatc aatgatttat
      181 ataaaagtgt tgcaaaaaga gagtggactg gaatatttga gatcgttaat aataacatca
      241 agcaatttag agatctgttc ccaaatccgg aaggctggat cgatgatggt caccaatgtt
      301 tcgctccttg ggttaaagaa actaaaaaac gcaatggcaa atatcatgtc tacgaccccc
      361 tcgccctaga tttggacgga gacggtatag aaaccgttgc caccaaaggc tttgcaggca
      421 gcttatttga tcacaccaac aacggtatcc gcaccgccac cggttgggtt gctgccgatg
      481 acggtctgct tgt
//
LOCUS       DAWFAD010000276          459 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.276, whole genome shotgun sequence.
ACCESSION   DAWFAD010000276 DAWFAD010000000
VERSION     DAWFAD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.276"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          110 a          122 c          115 g          112 t
ORIGIN      
        1 cgggaatccg gacttgtcgg cacggaaact tatcgggtac aacggtttct tgagattccg
       61 catcctagat tcccgcctgc gcgggaatga cggcgcataa gtttctgtgt tgatttgagt
      121 ctatgggaac ggtgtaggat tggtggaatc gttggaatcg gtgggctgaa gcccacccta
      181 taactcacct tttacgaatc ctcctacacc ctatacaaca ccttgaaaat ccaccttgtc
      241 gggaatgccc gaaccgtcag aaatacccga atcgtcattc ccgcgcaggc gggaatccag
      301 acctgtcggc acagaaactt atcgggcaaa acggtttctt gagattccgc atcctagatt
      361 cccgcctgcg cgggaatgac ggctgcagat gcccaacggt ctttattcct ccatctctaa
      421 gatttacagc gatacacggg tagtttaagg aatgtccga
//
LOCUS       DAWFAD010000277          452 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.277, whole genome shotgun sequence.
ACCESSION   DAWFAD010000277 DAWFAD010000000
VERSION     DAWFAD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.277"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1..452
                     /locus_tag="WGO49_002057"
                     /pseudo
     CDS             1..452
                     /locus_tag="WGO49_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          145 a          119 c          111 g           77 t
ORIGIN      
        1 ataccctgtc cgaattgctg gaactgatta actgccaact gaccgaaaaa ggcttaaaag
       61 tagagaaagc attgacgcca ccattattca gaccgctggc agcaaacagc gtcaggccat
      121 agaagttgac gaagaaggac aaatcagcgg ccaaaccaca ccgagtaaag acagcgatgc
      181 ccgttggata aagaaaaacg gcctctacaa actcggttac aaacaacata cccgtaccga
      241 tgtggaaggc tatatcgaga aactgcacat tacccccgcc aatgcccatg agtgcaaaca
      301 cctgccgcct ttgctggaag ggatagctga aggtacgacc gtctatgccg acaaaggcta
      361 tgacagtgcg gaaaaccggc aacatctgga agagcgtcag ttgttagacg gcattatgcg
      421 caaagcctgc cgcaaccgtc cgctgtcgga ag
//
LOCUS       DAWFAD010000278          452 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.278, whole genome shotgun sequence.
ACCESSION   DAWFAD010000278 DAWFAD010000000
VERSION     DAWFAD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.278"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          133 a          114 c           92 g          113 t
ORIGIN      
        1 taaagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttaatcca
       61 ctatatccgt ttgcgcccgc caagccgtgt tgcacagatg cgacacggct tttttctttc
      121 tgttgtcaga atgtaaattt tcgtcaaaaa acatcaaaaa tccccccccc ccccgaattt
      181 gcgtaaaata aagtgtaatc cctttgatca taagggaatg ttgacaatta gatcgattct
      241 cctaggctga ctttcgcaat aaatggggtt gtcaataatt gtgtatggtc gtttgccgtc
      301 cggcggccgt cattcccgcg aacgcaggaa tccagcaaca caatgcagca ggactttatc
      361 ggaaaaaacc gaaaccccac cgactgtcat tcccgcaaaa gctggaatcc aaaaacgcaa
      421 cgcaacagga atttatcgga aaaaacagaa cc
//
LOCUS       DAWFAD010000279          427 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.279, whole genome shotgun sequence.
ACCESSION   DAWFAD010000279 DAWFAD010000000
VERSION     DAWFAD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.279"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>427
                     /locus_tag="WGO49_002058"
     CDS             <1..>427
                     /locus_tag="WGO49_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Opacity protein opA54"
                     /protein_id="HEZ0307101.1"
                     /translation="PRVSVGYDFGGWRIAADYARYRKWNNNKYSVNTKNVQKNDNGNR
                     QDLKTENQENGTFHAVSSLGLSAIYDFKLNDKFDKFKPYIGVRVAYGHVRHSIDSTKK
                     TTNVVTVAGAANTAPTIYYAPETQNAYHESHSIRRLGLGV"
BASE COUNT          135 a          114 c           90 g           88 t
ORIGIN      
        1 cccccgggtg tcggtcggct acgactttgg cggctggaga atagcggcag attatgcccg
       61 ttacagaaaa tggaacaaca ataaatattc cgtcaacaca aaaaatgtgc aaaaaaacga
      121 caatggcaac aggcaagacc tgaagacgga aaatcaggaa aacggtacat tccacgccgt
      181 ttcttctctc ggcttatccg ccatttacga tttcaaactc aacgataaat tcgataaatt
      241 caaaccctat atcggtgtgc gcgtcgccta cggacacgtc agacacagca tcgattcgac
      301 caaaaaaaca acaaatgttg ttaccgtcgc cggtgctgct aacacagcac ctacgattta
      361 ttatgcacca gagacgcaaa acgcctatca cgaaagccac agcatccgcc gcttgggtct
      421 tggtgtc
//
LOCUS       DAWFAD010000280          422 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.280, whole genome shotgun sequence.
ACCESSION   DAWFAD010000280 DAWFAD010000000
VERSION     DAWFAD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.280"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>422
                     /locus_tag="WGO49_002059"
     CDS             <1..>422
                     /locus_tag="WGO49_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307102.1"
                     /translation="TNDTYDHLAKNIYQNLLFQTRLQPYLNQISFKMENDTFTLDFSG
                     LVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKV
                     LGQETVALLAKTSGTQADDILQNVGFGHNKNVSLYGNDG"
BASE COUNT          138 a           76 c           91 g          117 t
ORIGIN      
        1 accaacgata catacgacca tctcgccaaa aacatctacc aaaacctgtt gttccaaacc
       61 cgtttgcagc catatttgaa tcaaatcagt ttcaaaatgg aaaatgatac gttcactttg
      121 gattttagtg gtcttgttca agcatttaac catgtcaaag aaactaatcc gcaaaaagct
      181 tttgtggatt tggccgagat gcttgcatat ggcgaacttc gttcttggta tgaaggccga
      241 agactaatgg ccgattatgt ggaggaggca aaaaaagcag gtaaatttga agattaccag
      301 aaagtgttgg gtcaggagac cgttgcatta ttagctaaaa catcgggtac gcaagcagat
      361 gatatcctgc aaaatgtagg ctttggtcat aataaaaatg tttctttata tggtaatgac
      421 gg
//
LOCUS       DAWFAD010000281          417 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.281, whole genome shotgun sequence.
ACCESSION   DAWFAD010000281 DAWFAD010000000
VERSION     DAWFAD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.281"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..380
                     /locus_tag="WGO49_002060"
     CDS             <1..380
                     /locus_tag="WGO49_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0307103.1"
                     /translation="ERLAARDIIRFPGFVNNVSEVIKEHHIFVLPSYYREGVPRSTQE
                     AMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKMIYFIENREAVRLMGNAS
                     YAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          109 a           94 c          108 g          106 t
ORIGIN      
        1 tggaacgcct tgccgcccgc gatattatcc gtttccccgg ttttgtgaac aatgtttccg
       61 aagtgataaa agaacatcat atattcgtat tgccgtctta ttatagggaa ggcgttcccc
      121 gaagcactca ggaggcaatg gccgtcggca gggcagtgat tacgacggat gtccccggat
      181 gcagggaaac ggtcgccgac aaggtcaacg gcttcctgat cgaaccttgg aatccccgca
      241 tcttggccga aaaaatgatt tattttatcg aaaacaggga agccgtccgc ctgatgggga
      301 atgcaagtta tgcgattgcc aaagataaat tcgatgccga aaaagtcgat ttgaaattgc
      361 tcgatatttt gaaggcgtaa acaaggctgc ccgcttttga gtttcggtca tttctga
//
LOCUS       DAWFAD010000282          415 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.282, whole genome shotgun sequence.
ACCESSION   DAWFAD010000282 DAWFAD010000000
VERSION     DAWFAD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.282"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          152 a          115 c           83 g           65 t
ORIGIN      
        1 tcattcccgc aaaagcggga atccaaaaac gcaacgcaac aggaatccat cggaaaaaac
       61 cgaaacccca ccgaccgtca ttcccgcaaa agcgggaatc cagacccgtc ggcacggaaa
      121 cttaccggat aaaacagttt ccttagattc cacgtcctag attcccgcct tcgcgggaat
      181 gacgagattt tagattatgg gaatttatca ggaatgattg aatccataga aaaaccacag
      241 gaatctatca gaaaaaacag aaacccccca ccgcgtcatt cccgcgcagg cgggaatcca
      301 aaaacgcaac gcaacaggaa tttatcggaa aaaacagaaa cctcaccgcc gtcattcccg
      361 caaaagcggg aatctagaaa cacaacgcgg caggacttta tcagaaaaaa cagaa
//
LOCUS       DAWFAD010000283          385 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.283, whole genome shotgun sequence.
ACCESSION   DAWFAD010000283 DAWFAD010000000
VERSION     DAWFAD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.283"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            100..306
                     /locus_tag="WGO49_002061"
     CDS             100..306
                     /locus_tag="WGO49_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307104.1"
                     /translation="MFDGAKVVRRVEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSV
                     YLKQTGKGSNILQSSYSHKRFIVN"
BASE COUNT          123 a           64 c           89 g          109 t
ORIGIN      
        1 gttaacagat tatttttgca aaggtctcag attaggcggt gtgccgtaat cgtacgaatg
       61 ccgattcaac ctaagcagac atcagtattt aggaagtgga tgtttgatgg agcaaaggtt
      121 gtacgaaggg tggaaggcaa cctgtgggtg tttggtatgg tcgcgcttga aaaaacgtgt
      181 tttaagggac aaatgccgtc tgaaaatcgg tttcagacgg cattttctgt ttatttaaag
      241 caaacaggaa aaggcagcaa tattctgcag tcttcctatt cacacaagcg ttttatagtt
      301 aattaaaaac aaaatagtac aatactcaac tttgaaggtc taaccatggc atactctgcg
      361 gacttaagaa acaaagcttt aaact
//
LOCUS       DAWFAD010000284          380 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.284, whole genome shotgun sequence.
ACCESSION   DAWFAD010000284 DAWFAD010000000
VERSION     DAWFAD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.284"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..71
                     /locus_tag="WGO49_002062"
     CDS             <1..71
                     /locus_tag="WGO49_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0307105.1"
                     /translation="VKQGIYPHNWGGGNADFSIGYD"
     gene            108..380
                     /locus_tag="WGO49_002063"
                     /pseudo
     CDS             108..380
                     /locus_tag="WGO49_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           99 a           92 c          107 g           82 t
ORIGIN      
        1 atgtcaaaca gggtatttat ccgcataatt ggggtggcgg caatgcggac ttttctattg
       61 gatacgattg aagtaatgtc ggattcgaga atccgaccta cggaaaactg aaagagcatc
      121 ggctgcagga cggcattatg cgcaaagcct gccgcaaccg tccgctgtcg gaagtgcaaa
      181 ccaaacgtaa ccgatatttg tcgaagaccc gttatgtggt cgaacagagc ttcggtacgc
      241 tgcaccgtaa gttccgctat gcgcgggcag cctatttcgg actgattaaa gtgagtgcgc
      301 aaagccacct gaaggcgatg tgtttgaacc ttttgaaagc ggccaacagg ctaagtgcgc
      361 ccgctgccgc ccaaaaggcg
//
LOCUS       DAWFAD010000285          376 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.285, whole genome shotgun sequence.
ACCESSION   DAWFAD010000285 DAWFAD010000000
VERSION     DAWFAD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.285"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          102 a           83 c           80 g          111 t
ORIGIN      
        1 aagccctccc tacagagctt tcagacggca ttttttgcgt ttttcgggag ggggcaaatg
       61 aaacgccgac aaaccgatgc cgtcattccc gcgcaggcgg gaatctagga cgtaaaatct
      121 caagaaaccg ttgtacccga taagtttccg tgccgacaag tccggattcc cgccttatat
      181 gatgcgctct atcaaagggg cgcattaatt ttcttaacat tcccctttga cagccaagtg
      241 aaaggggctt ttttatgtca gcagtaaatg taatattttc ctgttcttat tggagaatat
      301 ttaaaaaatc agattcttgt gttttgtgtt tttatcagtt cagacatggc gaaccgcata
      361 aactcattaa tcaaga
//
LOCUS       DAWFAD010000286          366 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.286, whole genome shotgun sequence.
ACCESSION   DAWFAD010000286 DAWFAD010000000
VERSION     DAWFAD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.286"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          123 a           87 c           59 g           97 t
ORIGIN      
        1 gtcttttcta aaatatgcag atattcgaat atcgaatttg ccttatgatt acggttttct
       61 gtcttatctg ccttaaaacg ccggtattcc gtttgaacca aattatattt gccgaaacac
      121 tcaaaaatct cccttacctg cccgacagac atcaagcctt cgttgttgta gctgagaaaa
      181 atatattgaa agtccgcatc cctaatcaaa gcctcaaata ccggaaccac atccgatttt
      241 gagcaagtcc gcgctaaaac acatcgtaca gcgcaaaacc ggatgggaat ggcaaaatcc
      301 ggaaacaaac atttttcaaa gcatacaaac aaaaaaatac caaccggagg ttggtattcc
      361 ttaact
//
LOCUS       DAWFAD010000287          364 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.287, whole genome shotgun sequence.
ACCESSION   DAWFAD010000287 DAWFAD010000000
VERSION     DAWFAD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.287"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..283)
                     /locus_tag="WGO49_002064"
     CDS             complement(<1..283)
                     /locus_tag="WGO49_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEZ0307106.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPS"
BASE COUNT           97 a           91 c          108 g           68 t
ORIGIN      
        1 aagaaggaac gtatgtaccc gaaacggcag gtgcagacgg cggaacataa gcaggaggcg
       61 tataaaccgg cgcgctttgc accggcggca cataaggcgc atcgccggca ggagccgggc
      121 tgtacggcgt tgctccatag gggttgttgt aaactgccga agacggcgcg tcctgcatac
      181 ctgaattgcc tgcaatgaca ggagcaggct gttgggtggc gcaaccgccc aacagtgcgg
      241 caaaggaggc gcaagccgcc aaaagtgtcg tttgtttcaa cataagataa ccttcatgtt
      301 ccgatatata gcctgaatgc ggtatatcat aataaaaatg cgcgttcttc tcaagcgcaa
      361 agcc
//
LOCUS       DAWFAD010000288          360 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.288, whole genome shotgun sequence.
ACCESSION   DAWFAD010000288 DAWFAD010000000
VERSION     DAWFAD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.288"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            32..>360
                     /locus_tag="WGO49_002065"
     CDS             32..>360
                     /locus_tag="WGO49_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0307107.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSD"
BASE COUNT          112 a           97 c           86 g           65 t
ORIGIN      
        1 cgcacaaatt tagtcaatat caagaccaat tatgaaccaa ctcgaccaac ttggcacccg
       61 tatcaacctg atttgcaatg tcttcgacaa atggatcggg cagcaggatc tgaattacaa
      121 cctctttgcc gtactttata ccctggcaac cgaaggcagc cgcacgcaaa agcatatcgg
      181 cgaaaagtgg agcctgccca aacagaccgt ttcaggcgta tgcaaaaccc ttgccggaca
      241 agggttgatt gaatggcagg aaagcgaaca ggaccggcgc gaacggttgc tgtcgctgac
      301 cgaaaaaggc aaagcccatg ccgcaccttt aacagaaagc gcgcaggaat tcagcgacaa
//
LOCUS       DAWFAD010000289          354 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.289, whole genome shotgun sequence.
ACCESSION   DAWFAD010000289 DAWFAD010000000
VERSION     DAWFAD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.289"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           97 a           92 c           83 g           82 t
ORIGIN      
        1 aggcttacat caaagccgat aacgctttca caaagcctgt gtgcactgaa aactgaaagg
       61 tcgtctgaaa acggagcttt cgaaggaagc agagtttctg cgaagctaaa accgaagctg
      121 caaaaaatcg aaaagcagcc tgcacgttga aaatgccgcc caagcaaacc tgcggtttgc
      181 cgccctctcc ctagccctct cccacaggga gaggggattg gggtgcaggc tgtctttaag
      241 gttcaggcaa atttttaact tcgttgaaac tgcgatttca gaagctccgt tttagcttcg
      301 cagaaactcc gcttccttcg aaagctccgt tttcagacga ccttttggaa cacc
//
LOCUS       DAWFAD010000290          342 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.290, whole genome shotgun sequence.
ACCESSION   DAWFAD010000290 DAWFAD010000000
VERSION     DAWFAD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.290"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           84 a           87 c           76 g           95 t
ORIGIN      
        1 tattgccgtt gcccgacggt ttggattccc gcttgcgcgg gaatgacggt agagcggttt
       61 cagttgctcc cgataaatgc cgcaatctca aatcccgtca ttccctcaaa aacagaaaaa
      121 caaaatcaga aacctaaaat cccgtcattc ccgcagctct tcgtcattcc cgtgaaagcg
      181 ggaatctaga ccttttaaat tttcacacgg cttttgaata ttgccgttac ccgaaggtct
      241 ggattcccgc ctgcacggga atgacggcag agcggtttca gttgtttcta aataaatttt
      301 tgcagcgttg gatttttaga ttcccgcttt cgcgggaatg ac
//
LOCUS       DAWFAD010000291          336 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.291, whole genome shotgun sequence.
ACCESSION   DAWFAD010000291 DAWFAD010000000
VERSION     DAWFAD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.291"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           90 a           65 c           90 g           91 t
ORIGIN      
        1 tttttgacac attttcatga tttcaaatcc cgttattccc aagtggacgg aaacccgata
       61 atgggaagcc gcagagactt atcgggaaaa cggcaagccc tccgccgtca ttcccgcgca
      121 ggcgggaatc tagaaataaa aaaccacggt gttatcggca atgactgaaa ccggagaaat
      181 gacgaaattg aagcgtgcgg aaatgtgtcg gaaatcgctg aagccggata aactagattc
      241 ccgcttttgc gggaatgacg ggattttagg tttttggttt gttttcgcgg caatgatggg
      301 attttaggtt tttggtttgt tttcacggca atgatg
//
LOCUS       DAWFAD010000292          333 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.292, whole genome shotgun sequence.
ACCESSION   DAWFAD010000292 DAWFAD010000000
VERSION     DAWFAD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.292"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT          100 a           68 c           80 g           85 t
ORIGIN      
        1 tcattcccgc aaaagcggga atctagaaat ttaatgttgc ggtgatttat cggaaatgac
       61 tgaaactcaa cgaaccggat tcccactttc gcgggaatga cgaagtggaa gttacccgaa
      121 acccaaaaca agcgaaaccg aacggactgg attcccgcct gcgcgggaat gacgagattt
      181 taggtttcta tttttggttt tctgttctcg cgggaataac ggaattttaa gttttaggaa
      241 tttgtcggaa aaacagaaat cccccgccgt cattcccgca aaagcgggaa tctagaaatt
      301 taacgttgcg gtgatttatc ggaaatgact gaa
//
LOCUS       DAWFAD010000293          332 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.293, whole genome shotgun sequence.
ACCESSION   DAWFAD010000293 DAWFAD010000000
VERSION     DAWFAD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.293"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           96 a           73 c           71 g           92 t
ORIGIN      
        1 tttttgaatt tcgggcaacg cttgaatctt cattccgcgc aggcggaaat tatcggtgcg
       61 gtacggcaac ttttttcgat atgaaaagac cgtcattcct gtaaaaacaa aaaatcaaaa
      121 acagaaaatt gaaattcgtc attcccgcgc aggcgggaat ctaggacgta aaatctatag
      181 tggattaaca aaaaccagta cagcgttgcc ttgacttagc tcgaagagaa cgattctcta
      241 aggtgctgaa gcaccgagtg agtcggttcc gcactatccg tactgtctgc ggcttcgtcg
      301 ctttctcctg atttttgtta atccactata aa
//
LOCUS       DAWFAD010000294          326 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.294, whole genome shotgun sequence.
ACCESSION   DAWFAD010000294 DAWFAD010000000
VERSION     DAWFAD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.294"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           90 a           55 c           73 g          108 t
ORIGIN      
        1 tttctgtttt tgattttctg ttttcgtagg aatgacggtt taaaagttac ccgaaacctc
       61 aaaaaaaagg ctgtgtttta atatagtgtt gatagacgta cttggcttcc atgtattcaa
      121 tcgtggaaat ctatatcttc gtcctcgccg aaatagtcta tgcccgatat acaattttga
      181 tacacaaact tggaaatatg ggtatcgtcg ccggagcgat agaatgcgga cagttatata
      241 tatacggttt ttttagggaa gcgggcagat gaagggcgga ttttgaatta accccatgca
      301 aattgacttt tgcaggcggt tttacc
//
LOCUS       DAWFAD010000295          323 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.295, whole genome shotgun sequence.
ACCESSION   DAWFAD010000295 DAWFAD010000000
VERSION     DAWFAD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.295"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           81 a           81 c           73 g           88 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta tatgaatcgt cattcccgcg caggcgggaa
       61 tctagacatt caatgctaag gcaatttatt gaaaatgact gaaactcaaa aaactagatt
      121 cccactttcg tgggaatgac gtggtgcagg tttccgtacg gatggattcg tcattcccgc
      181 gcaggcggga atctagacct gtcggtgcgg aaacttatcg gataacacgg tttccccaac
      241 cctgcatcct ggattcccac tttcgtggga atgacggcgg agcggtttct gctttttcca
      301 ataaatgccc cccaaactaa aat
//
LOCUS       DAWFAD010000296          321 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.296, whole genome shotgun sequence.
ACCESSION   DAWFAD010000296 DAWFAD010000000
VERSION     DAWFAD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.296"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           70 a           76 c          103 g           72 t
ORIGIN      
        1 gcaaagccat ctgaaggcga tgtgtttgaa cctgttgaaa gccgccaaca ggctaagtgc
       61 gcctgttgcc gcctaaaagg cagcacggat gcctgattat cgggtatccg gggaggatta
      121 agggggtatt ggggtaaaat taggaggtat ttggggcgga aatagctgaa aacctgtgtt
      181 tgggtttcgg ctgtcgggaa ggaaaggaat tttgcaaagg tctcgggcta tgccggctgc
      241 tgccgcccat tgcaccttcg ccgaatcctg ccgcccaccg ccgaagccct catgcgttcg
      301 cggtacagcg cgtatgtgct a
//
LOCUS       DAWFAD010000297          319 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.297, whole genome shotgun sequence.
ACCESSION   DAWFAD010000297 DAWFAD010000000
VERSION     DAWFAD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.297"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            1..263
                     /locus_tag="WGO49_002066"
                     /pseudo
     CDS             1..263
                     /locus_tag="WGO49_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           96 a           95 c           68 g           60 t
ORIGIN      
        1 aactcgaaca cagcctcatc acccgcatcg atttcaacct gttttgccgt tttgacgaac
       61 tgagcatccc tgattacagc accttatgcc gctaccgcaa ttggctggcg caagacgaca
      121 ccctgtccga attgctcaaa ctgatcaacc gccaactgac cgaaaaaggt ttaaaaatag
      181 agaaagcatc cgctgccgtc gttgacgcca ccattattca gaccgccggc agcaaacagc
      241 gtcaggccat agaagttgac gaataaggac aaatcaacgg ccaaaccaca ccgagtaagg
      301 acagcgatgc ccgttggat
//
LOCUS       DAWFAD010000298          305 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.298, whole genome shotgun sequence.
ACCESSION   DAWFAD010000298 DAWFAD010000000
VERSION     DAWFAD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.298"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(1..86)
                     /locus_tag="WGO49_002067"
                     /pseudo
     CDS             complement(1..86)
                     /locus_tag="WGO49_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
BASE COUNT           79 a           51 c          105 g           70 t
ORIGIN      
        1 gctaaaaaat aggggaacct gtaaaggaag gggcatcggc cgccgtcggt gcttttttgt
       61 ttggaaggga aatggctgga aaaagacctg cgctatatcg gggaatgaat cgacgcaaag
      121 aaaatgccgt ctgaacatgc gttcgggcgg cgttttgttg gggggtatcg gagcggaacg
      181 tctgaaaaag ggtttcaggc ggtctttggg cgtgtggtga cagtcgaaaa cgtgataagg
      241 ctacctgaaa agtttgggag attttcaggt agcctttggt attgggcgca acagacgcag
      301 gtaca
//
LOCUS       DAWFAD010000299          302 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.299, whole genome shotgun sequence.
ACCESSION   DAWFAD010000299 DAWFAD010000000
VERSION     DAWFAD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.299"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           95 a           73 c           49 g           85 t
ORIGIN      
        1 acggaaacct gcaccacgtc attcccacga acctacatcc catcattccc acaaggacag
       61 aaaaccaaaa acagaaacct aaaatcccgt cattcccgcc ttatatgatg cgctctatca
      121 aaggggcgca ttacttttct taacattccc ctttgacagc caagtgaaag gggctttttt
      181 atgtcagtag caaatgtaat attttcttgt tcctattgga gaatatttaa aaaatcagat
      241 tattgcgttt tatgtttcta tcagttcagg catggtgaac cgcataaact cgctgaacaa
      301 ga
//
LOCUS       DAWFAD010000300          298 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.300, whole genome shotgun sequence.
ACCESSION   DAWFAD010000300 DAWFAD010000000
VERSION     DAWFAD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.300"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>298
                     /locus_tag="WGO49_002068"
     CDS             <1..>298
                     /locus_tag="WGO49_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307108.1"
                     /translation="AAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETDSNWGK
                     KSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSDKAKAAIDAARDRIAVLD"
BASE COUNT           88 a           73 c           68 g           69 t
ORIGIN      
        1 gctgccgcat tgtccggcga tttggccaat atgctgaaag cttattctgc cgccgaaact
       61 aaagaagcac agttggcatt gttagataat ttgattcaca aatgggcgga aaccgattcg
      121 aactggggca aaaaatcgcc aatgcgactt tcaaccgatt ggacgcaaac ggctaatgaa
      181 ggtattgcac tgacaccatc ccaagtagca caactaaaaa agaacgcttt agtttccctt
      241 tctgataaag ctaaagcagc tattgacgcc gcccgcgacc gcattgccgt gcttgatg
//
LOCUS       DAWFAD010000301          286 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.301, whole genome shotgun sequence.
ACCESSION   DAWFAD010000301 DAWFAD010000000
VERSION     DAWFAD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.301"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>286
                     /locus_tag="WGO49_002069"
     CDS             <1..>286
                     /locus_tag="WGO49_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0307109.1"
                     /translation="LLPLLEGIAEGTTVYADKGYDSKENRQHLKEHQLLDGIMRKACR
                     NRPLSETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLLKVSAQSH"
BASE COUNT           76 a           77 c           77 g           56 t
ORIGIN      
        1 cctgttgcct ttgctggaag gtatagccga aggcacgacc gtctatgccg acaaaggcta
       61 cgacagtaag gaaaaccggc agcatctgaa agaacatcag ttactggacg gcattatgcg
      121 caaagcctgc cgcaaccgtc cgctgtcgga aacgcaaacc aaacgcaacc ggtatttgtc
      181 gaagacccgt tatgtggttg aacagagctt cggtacgctg caccgtaaat tccgctatgc
      241 gcgggcagcc tatttcgggc tgctcaaagt gagtgcgcaa agccat
//
LOCUS       DAWFAD010000302          279 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.302, whole genome shotgun sequence.
ACCESSION   DAWFAD010000302 DAWFAD010000000
VERSION     DAWFAD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.302"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           93 a           65 c           67 g           54 t
ORIGIN      
        1 tggatggatt cgtcgttccc gcgaaagcgg gaatccggaa ccagaaacca caaaaattta
       61 tcgggaaaac cgaaactcaa aaacaacgga aaccgaacgg acaggattcc cgcctgcgcg
      121 ggaatgacgg ctcatacatc acccaaaaaa ttgaaaccaa acagatggga ttccctcttg
      181 cgtgaggctg acagatgccg tctgaaagac tttcagacgg catagctttt tctctttgaa
      241 tttatagtgg attaacaaaa atcaggacaa ggcggcgag
//
LOCUS       DAWFAD010000303          277 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.303, whole genome shotgun sequence.
ACCESSION   DAWFAD010000303 DAWFAD010000000
VERSION     DAWFAD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.303"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>277
                     /locus_tag="WGO49_002070"
     CDS             <1..>277
                     /locus_tag="WGO49_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0307110.1"
                     /translation="CRFDELIIPDYSTLCRYRNWLAQDDTLSELLKLINCQLTEKGLK
                     IEKASAAVVDATIIQTAGSKQRQAIEVDEEGQISGQTTPSKDSDARW"
BASE COUNT           84 a           81 c           63 g           49 t
ORIGIN      
        1 tttgccgttt cgacgaactg atcatccccg attacagtac cttatgccgc taccgcaact
       61 ggctggcgca agacgacacc ctgtccgaat tactcaaact gattaactgc caactgaccg
      121 aaaaaggcct aaaaatagag aaagcatccg ccgccgtcgt tgatgccacc attatccaga
      181 ccgccggcag caaacagcgt caggccatag aagttgacga agaaggacaa atcagcggtc
      241 aaaccacacc gagtaaggac agcgatgccc gttggat
//
LOCUS       DAWFAD010000304          275 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.304, whole genome shotgun sequence.
ACCESSION   DAWFAD010000304 DAWFAD010000000
VERSION     DAWFAD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.304"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           63 a           62 c           56 g           94 t
ORIGIN      
        1 tctaaaaaat ttatgctatt aaatcagtaa tttctgatga attttgaaaa cttaatcccg
       61 tcattcccgc gcaagcggga atccggctcg ttcggtttcg cttgttttaa gtttcgggta
      121 acttccactt cgtcattccc gcacaggcgg gaatctggtt cgttcggtct cgcttgtttt
      181 aagtttcggg taacttccac ttcgtcattc ccgcgcaggc gggaatccaa tgcgttgagt
      241 ttcagctatt tagaataaat tttgaaactc taatc
//
LOCUS       DAWFAD010000305          274 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.305, whole genome shotgun sequence.
ACCESSION   DAWFAD010000305 DAWFAD010000000
VERSION     DAWFAD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.305"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            34..>274
                     /locus_tag="WGO49_002071"
     CDS             34..>274
                     /locus_tag="WGO49_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEZ0307111.1"
                     /translation="MKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKA
                     IAEYRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQAS"
BASE COUNT           60 a           65 c           85 g           64 t
ORIGIN      
        1 ggtttattgt ttgcaaccct taaaggaaaa accatgaaga aaatgttcgt gctgttctgt
       61 atgctgttct cctgcgcctt ctcccttgcg gcggtaaaca tcaatgcggc ttcgcagcag
      121 gagttggagg cgctgccggg cataggccct gcgaaggcga aggccattgc ggaataccgt
      181 gcgcaaaacg gtgcgttcaa gtctgtggac gatttgacca aggtaaaggg catcggccct
      241 gcggtgctgg cgaagctgaa ggatcaggct tctg
//
LOCUS       DAWFAD010000306          265 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.306, whole genome shotgun sequence.
ACCESSION   DAWFAD010000306 DAWFAD010000000
VERSION     DAWFAD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.306"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           79 a           61 c           50 g           75 t
ORIGIN      
        1 tgacggcggg gggatttctg tttttccgat aaattcctaa aacttaaaat tccgttattt
       61 ccgcgagaac agaaaaccaa aaacagaaac ctaaaatctc gtcattccca cgcaggcggg
      121 aatccagtct gttgagtttc agtcatttcc gataaatcac cgcaacatta aatttccaga
      181 ttcccgcctg cgcgggaatg acggtggggg gatttctgtt tttccgacaa attcctaaaa
      241 cttaaaattt catcatttct acgaa
//
LOCUS       DAWFAD010000307          265 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.307, whole genome shotgun sequence.
ACCESSION   DAWFAD010000307 DAWFAD010000000
VERSION     DAWFAD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.307"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..158
                     /locus_tag="WGO49_002072"
     CDS             <1..158
                     /locus_tag="WGO49_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0307112.1"
                     /translation="VEQSFGTLHRKFRYARVAYFGLIKVSAQSHLKAMCLNLLKAANR
                     LSAPAAA"
BASE COUNT           61 a           58 c           87 g           59 t
ORIGIN      
        1 tggtcgaaca gagcttcggt acgctgcacc gtaaattccg ctatgcccgg gtagcctatt
       61 tcgggctgat taaagtgagt gcgcaaagcc acctgaaggc gatgtgtttg aacctgttga
      121 aagcggccaa caggctaagt gcgcccgctg ccgcctaaaa agcggcccgg atgcctgatt
      181 atcgggtgtc cgggcaggat taagggggtg tttgggtaaa attaggcggt atttggggcg
      241 aaaacagctg aaaacctgtg ttggg
//
LOCUS       DAWFAD010000308          263 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.308, whole genome shotgun sequence.
ACCESSION   DAWFAD010000308 DAWFAD010000000
VERSION     DAWFAD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.308"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            31..201
                     /locus_tag="WGO49_002073"
     CDS             31..201
                     /locus_tag="WGO49_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ0307113.1"
                     /translation="MAQYMCGPCGWIYDEEHGDPEHGIPPGTKFEDIPDDWKCPECGV
                     GKEDFYLLDFVI"
BASE COUNT           72 a           76 c           71 g           44 t
ORIGIN      
        1 atccgctata caaaaaacaa ggaaacacaa atggcgcaat atatgtgcgg cccctgcggc
       61 tggatttacg atgaagaaca cggcgacccc gaacacggca tcccccctgg gacaaagttt
      121 gaagacatcc ccgacgactg gaaatgcccc gaatgcggcg tgggcaaaga agatttctac
      181 ctgttggatt tcgtgatata gcggcagccg cacccttagg gaaaaccccg tcatccggcg
      241 ttccgcctgc ggcggcaggg aac
//
LOCUS       DAWFAD010000309          250 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.309, whole genome shotgun sequence.
ACCESSION   DAWFAD010000309 DAWFAD010000000
VERSION     DAWFAD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.309"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           68 a           69 c           47 g           66 t
ORIGIN      
        1 tcagatttcc gtgtttcgcg tttccggcaa ttcaagattg gcaaaacacg tcagcctgct
       61 tcgcaaggca ggtttttaaa actttatcag gcgttcgggc atatcggttc gaccggtccg
      121 catttccggc aatcaacact gccgacggtt gccgcatttc tttttgatat acaatcacac
      181 tttcaaaaaa catacgcagg caacaaaatc atcctccccc gattggcaaa cattccggca
      241 aattaaaacc
//
LOCUS       DAWFAD010000310          246 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.310, whole genome shotgun sequence.
ACCESSION   DAWFAD010000310 DAWFAD010000000
VERSION     DAWFAD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.310"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>246
                     /locus_tag="WGO49_002074"
     CDS             <1..>246
                     /locus_tag="WGO49_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0307114.1"
                     /translation="AGKVVVFGILKRNRRVYTVVVDNAKSETLLPVIKKKIMPDSIVY
                     TDSLSSYDKLDVSGFIHYRINHSKEFADRQNHINGIEN"
BASE COUNT           75 a           53 c           56 g           62 t
ORIGIN      
        1 gcaggaaaag tggttgtttt cggcattctg aaacgcaaca gacgggtcta taccgttgtg
       61 gtggataatg ccaagtctga aacgttactc cctgtcatca aaaagaaaat catgccggat
      121 agtattgttt ataccgatag tctgagcagc tacgacaagt tggacgtgag cggttttatc
      181 cattaccgca tcaaccattc caaggaattt gcagaccgtc aaaaccacat taacggcatt
      241 gagaac
//
LOCUS       DAWFAD010000311          245 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.311, whole genome shotgun sequence.
ACCESSION   DAWFAD010000311 DAWFAD010000000
VERSION     DAWFAD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.311"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           68 a           53 c           65 g           59 t
ORIGIN      
        1 catcattccc gcaaaagcgg gaatctagaa atgaaaagca acaggaattt atcggaaatg
       61 accgaaactg aacggactgg attccctctt ttgcgggaat gacggcgaca gggttgctgt
      121 tatagtggat gaacaaaaac cggtacggcg ttgtctcgcc ttagttcgaa gagaacgatt
      181 ctctaaggtg ctgaagcacc aagtgaatcg gttccgtact atctgtactg tctgcggctt
      241 cgtcg
//
LOCUS       DAWFAD010000312          241 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.312, whole genome shotgun sequence.
ACCESSION   DAWFAD010000312 DAWFAD010000000
VERSION     DAWFAD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.312"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>241
                     /locus_tag="WGO49_002075"
     CDS             <1..>241
                     /locus_tag="WGO49_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0307115.1"
                     /translation="VDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFY
                     GENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMS"
BASE COUNT           69 a           42 c           49 g           81 t
ORIGIN      
        1 atgtggataa aaactctgac actatgcact ttaaaggatt taaagcagca gatgttcatt
       61 ttatccgttc cggaagtgat ttggtgctta gcgcttctga acaagacaac gtacgtattt
      121 ccggattctt ctatggtgaa aaccatcgtg tagatacatt tgtctttgat gatgcagcta
      181 tcagtaatcc agattttgcc aagtatatta atgctggcaa taatttggta cagtctatgt
      241 c
//
LOCUS       DAWFAD010000313          234 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.313, whole genome shotgun sequence.
ACCESSION   DAWFAD010000313 DAWFAD010000000
VERSION     DAWFAD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.313"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           69 a           58 c           48 g           59 t
ORIGIN      
        1 aacaaaaacc ggtacggcgt tgtctcgcct tagctcaaag agaacgattc tctaaggtgc
       61 tgaagcacga gtgaatcggt tccgtaccat ctgtactgtc tgcggcttcg tcgccttgtc
      121 ctgatttttg tgaatccact atagaatttc aatgcctcaa gaatttatcg gaaaaaacca
      181 aaacccttcc gccgtcattc ccacaaaagt gggaatctag aaatgaaaag cagc
//
LOCUS       DAWFAD010000314          234 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.314, whole genome shotgun sequence.
ACCESSION   DAWFAD010000314 DAWFAD010000000
VERSION     DAWFAD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..234
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.314"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           83 a           57 c           47 g           47 t
ORIGIN      
        1 ctttaatcag tcggacaaaa tgcctttatc cgatggaacc gttttccgcc cgaacggaaa
       61 taagcggttt tcccctgtaa acagataaaa ccaaccgggt attcatacac agccaaaaaa
      121 aagccgtccg aacccaaagg ggacagacgg ccaaacacat tacggggaaa acgtcttact
      181 caatgagtct gcgaaacaga cattgctaag acaactgcaa ttataaggta agtt
//
LOCUS       DAWFAD010000315          228 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.315, whole genome shotgun sequence.
ACCESSION   DAWFAD010000315 DAWFAD010000000
VERSION     DAWFAD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.315"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           80 a           54 c           44 g           50 t
ORIGIN      
        1 gaaactgaac gaattagatt cccgcctgcg cgggaatgac aactcattag ttacctaaca
       61 cttaaaaaac agaaaccttt ccgcgtcatt cccacgaaag tgggaatccg ggaacttaac
      121 gttacagtga tttatcggaa acgactgaaa ccgaagagac cggattcccg cctgcgtggg
      181 aatgacaact cattagttac ttaaaactta aaaaacagaa acctttac
//
LOCUS       DAWFAD010000316          227 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.316, whole genome shotgun sequence.
ACCESSION   DAWFAD010000316 DAWFAD010000000
VERSION     DAWFAD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.316"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           65 a           62 c           52 g           48 t
ORIGIN      
        1 gtcatttcca taaaaaacag caacccgaaa cagcaactta aaaacagcaa cctgaaacct
       61 cgtcattccc gcgcaggtgg gaatccagat cattgggtgg cggcaatgtt caaaagtcgt
      121 ctgaaaaatc agaagttcta gattcccgtt ttcacgggaa tgacggaatt tcagacggca
      181 tctgcccgcc ccgtcattcc cgcgcaggcg ggaatctagt ccgttcg
//
LOCUS       DAWFAD010000317          222 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.317, whole genome shotgun sequence.
ACCESSION   DAWFAD010000317 DAWFAD010000000
VERSION     DAWFAD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.317"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           62 a           61 c           51 g           48 t
ORIGIN      
        1 aagccgtagg tcggatactt gtatccgaca aaagcctgcc atctcaaata gccgtcggat
       61 tcgagaatcc gacctgccaa accgggcgcg gacgctccgg ccggcagtta gtacgcaaat
      121 cgaacagaac atcacaaaaa agcccgattc ggattttcca atcgggcttt tttgcgcccg
      181 ttttgtcatc ccgtgaaata tccgcatgac aaaaatatag tg
//
LOCUS       DAWFAD010000318          222 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.318, whole genome shotgun sequence.
ACCESSION   DAWFAD010000318 DAWFAD010000000
VERSION     DAWFAD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.318"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            complement(<1..83)
                     /locus_tag="WGO49_002076"
     CDS             complement(<1..83)
                     /locus_tag="WGO49_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0307116.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILT"
BASE COUNT           59 a           39 c           53 g           71 t
ORIGIN      
        1 tcggtcaaaa tcttctgccg tttattggtt acaaccgtaa aaaagtaagt gccgccattg
       61 cggtaaaaac gacggtattt catagtatta tgctcggaat gattttgtag gtcggattct
      121 tgaattcgac attttgggca ttgctgcaat ggattgcaat gatgggaatg ttaaaggttt
      181 tgtcggatac aagtatccga cctacgcttg ctgaaccgtc at
//
LOCUS       DAWFAD010000319          215 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.319, whole genome shotgun sequence.
ACCESSION   DAWFAD010000319 DAWFAD010000000
VERSION     DAWFAD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.319"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           61 a           52 c           53 g           49 t
ORIGIN      
        1 tccgtttccg aaaatgacgg tagaaaagga tacaggctaa aaataaaggc agcagaaagc
       61 gcgcataggg ataccacgga tggtctgcat gccagtatcc gtgccgtccc tgtggaacgg
      121 tatcatagcg gtaactgtcg gggaaaaagt tgatccaaac cgttactgcc agccataagg
      181 ctatatccct gactgctttc atttacttct gctcc
//
LOCUS       DAWFAD010000320          210 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.320, whole genome shotgun sequence.
ACCESSION   DAWFAD010000320 DAWFAD010000000
VERSION     DAWFAD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..210
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.320"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>210
                     /locus_tag="WGO49_002077"
     CDS             <1..>210
                     /locus_tag="WGO49_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221999.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HEZ0307117.1"
                     /translation="NGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMF
                     EHNAKLDRWGNSMEFINGVAAGALNP"
BASE COUNT           60 a           76 c           47 g           27 t
ORIGIN      
        1 aacggcacag cccgcagtat caaactcaat ccgaccgaca cccgcagcat ccggcaacgc
       61 atatccgaca actacaacaa cctcggcagc aatttctccg accgcgccga tgaagccaac
      121 agaaaaatgt tcgagcacaa tgccaagctc gaccgctggg gcaacagcat ggagtttatc
      181 aacggcgtcg ccgccggcgc gctcaacccc
//
LOCUS       DAWFAD010000321          207 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.321, whole genome shotgun sequence.
ACCESSION   DAWFAD010000321 DAWFAD010000000
VERSION     DAWFAD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..207
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.321"
                     /strain="W"
                     /db_xref="taxon:487"
     gene            <1..>207
                     /locus_tag="WGO49_002078"
     CDS             <1..>207
                     /locus_tag="WGO49_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0307118.1"
                     /translation="AKAIRRAGIAVIIANPRQTHQFAQSQPLTKTDAKDAKMPAFFAQ
                     MTAQKEDSQTMPYQPPTEAEEVLEA"
BASE COUNT           61 a           71 c           48 g           27 t
ORIGIN      
        1 gccaaagcca tccgccgagc aggcatagcc gtgattatcg ccaacccgcg tcagacgcat
       61 cagtttgccc aatcgcagcc gctgaccaaa accgacgcca aagatgccaa aatgcccgcc
      121 ttcttcgcac agatgacggc acagaaagaa gattcgcaaa ccatgcccta ccaaccgccc
      181 accgaagcgg aagaagtgtt ggaagcc
//
LOCUS       DAWFAD010000322          205 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain W
            SAMEA2060828-rid20301203.denovo.322, whole genome shotgun sequence.
ACCESSION   DAWFAD010000322 DAWFAD010000000
VERSION     DAWFAD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2060828
            Sequence Read Archive: ERR332342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001995185.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001995185.1
            Genome Coverage       :: 206x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/22/2023 17:58:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,078
            CDSs (total)                      :: 2,022
            Genes (coding)                    :: 1,945
            CDSs (with protein)               :: 1,945
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 29 of 77
            Pseudo Genes (incomplete)         :: 40 of 77
            Pseudo Genes (internal stop)      :: 15 of 77
            Pseudo Genes (multiple problems)  :: 6 of 77
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2060828-rid20301203.denovo.322"
                     /strain="W"
                     /db_xref="taxon:487"
BASE COUNT           64 a           46 c           45 g           50 t
ORIGIN      
        1 atggatacag cgatgaagta gtgcgacaac atagacaagg gcaaccttga ttcacactac
       61 cataaccgct tgctaccaag gaaaacaaaa tgaatttgcc tattcaaaaa ttcatgatgc
      121 tgtttgcagc agcaatatcg ttgctgcaaa tccccattag tcatgcgaac ggtttggatg
      181 cccgtttgcg cgatgatatg caggc
//
